LOCUS       DAZKOB010000001       114431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000001 DAZKOB010000000
VERSION     DAZKOB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 114431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 114431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..114431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.001"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(258..2444)
                     /locus_tag="ACG8M6_000001"
     CDS             complement(258..2444)
                     /locus_tag="ACG8M6_000001"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0455706.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2662..3345
                     /locus_tag="ACG8M6_000002"
     CDS             2662..3345
                     /locus_tag="ACG8M6_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0455707.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3426..4145)
                     /locus_tag="ACG8M6_000003"
     CDS             complement(3426..4145)
                     /locus_tag="ACG8M6_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0455708.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4457..4663)
                     /locus_tag="ACG8M6_000004"
     CDS             complement(4457..4663)
                     /locus_tag="ACG8M6_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455709.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4908..5510
                     /locus_tag="ACG8M6_000005"
     CDS             4908..5510
                     /locus_tag="ACG8M6_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0455710.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5651..6931
                     /locus_tag="ACG8M6_000006"
     CDS             5651..6931
                     /locus_tag="ACG8M6_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0455711.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6944..7672
                     /locus_tag="ACG8M6_000007"
     CDS             6944..7672
                     /locus_tag="ACG8M6_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0455712.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7741..9264)
                     /locus_tag="ACG8M6_000008"
     CDS             complement(7741..9264)
                     /locus_tag="ACG8M6_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0455713.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9516..10697
                     /locus_tag="ACG8M6_000009"
     CDS             9516..10697
                     /locus_tag="ACG8M6_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0455714.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10757..12385)
                     /gene="treC"
                     /locus_tag="ACG8M6_000010"
     CDS             complement(10757..12385)
                     /gene="treC"
                     /locus_tag="ACG8M6_000010"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0455715.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12836..15547)
                     /locus_tag="ACG8M6_000011"
     CDS             complement(12836..15547)
                     /locus_tag="ACG8M6_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0455716.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15557..16258)
                     /locus_tag="ACG8M6_000012"
     CDS             complement(15557..16258)
                     /locus_tag="ACG8M6_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0455717.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16444..17043
                     /locus_tag="ACG8M6_000013"
     CDS             16444..17043
                     /locus_tag="ACG8M6_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0455718.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17124..18137
                     /locus_tag="ACG8M6_000014"
     CDS             17124..18137
                     /locus_tag="ACG8M6_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0455719.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18245..19162
                     /gene="rbsK"
                     /locus_tag="ACG8M6_000015"
     CDS             18245..19162
                     /gene="rbsK"
                     /locus_tag="ACG8M6_000015"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0455720.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19190..19585
                     /gene="rbsD"
                     /locus_tag="ACG8M6_000016"
     CDS             19190..19585
                     /gene="rbsD"
                     /locus_tag="ACG8M6_000016"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0455721.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19585..20958
                     /locus_tag="ACG8M6_000017"
     CDS             19585..20958
                     /locus_tag="ACG8M6_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0455722.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21315..23459
                     /locus_tag="ACG8M6_000018"
     CDS             21315..23459
                     /locus_tag="ACG8M6_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0455723.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23511..24551)
                     /locus_tag="ACG8M6_000019"
     CDS             complement(23511..24551)
                     /locus_tag="ACG8M6_000019"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0455724.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24828..26630)
                     /locus_tag="ACG8M6_000020"
     CDS             complement(24828..26630)
                     /locus_tag="ACG8M6_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0455725.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26644..27396)
                     /locus_tag="ACG8M6_000021"
     CDS             complement(26644..27396)
                     /locus_tag="ACG8M6_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0455726.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27405..27767)
                     /locus_tag="ACG8M6_000022"
     CDS             complement(27405..27767)
                     /locus_tag="ACG8M6_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0455727.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27943..28815)
                     /locus_tag="ACG8M6_000023"
     CDS             complement(27943..28815)
                     /locus_tag="ACG8M6_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0455728.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28866..29354)
                     /locus_tag="ACG8M6_000024"
     CDS             complement(28866..29354)
                     /locus_tag="ACG8M6_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0455729.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29492..30283)
                     /locus_tag="ACG8M6_000025"
     CDS             complement(29492..30283)
                     /locus_tag="ACG8M6_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0455730.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30338..30946)
                     /locus_tag="ACG8M6_000026"
     CDS             complement(30338..30946)
                     /locus_tag="ACG8M6_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0455731.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30939..31256)
                     /locus_tag="ACG8M6_000027"
     CDS             complement(30939..31256)
                     /locus_tag="ACG8M6_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0455732.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31412..32140)
                     /locus_tag="ACG8M6_000028"
     CDS             complement(31412..32140)
                     /locus_tag="ACG8M6_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0455733.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32299..33180
                     /locus_tag="ACG8M6_000029"
     CDS             32299..33180
                     /locus_tag="ACG8M6_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0455734.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33417..34460)
                     /locus_tag="ACG8M6_000030"
     CDS             complement(33417..34460)
                     /locus_tag="ACG8M6_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0455735.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34463..35131)
                     /locus_tag="ACG8M6_000031"
     CDS             complement(34463..35131)
                     /locus_tag="ACG8M6_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0455736.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35273..37609
                     /locus_tag="ACG8M6_000032"
     CDS             35273..37609
                     /locus_tag="ACG8M6_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0455737.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37724..38035)
                     /locus_tag="ACG8M6_000033"
     CDS             complement(37724..38035)
                     /locus_tag="ACG8M6_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455738.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            38216..39691
                     /locus_tag="ACG8M6_000034"
     CDS             38216..39691
                     /locus_tag="ACG8M6_000034"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0455739.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39738..40322
                     /locus_tag="ACG8M6_000035"
     CDS             39738..40322
                     /locus_tag="ACG8M6_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0455740.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40324..40743
                     /locus_tag="ACG8M6_000036"
     CDS             40324..40743
                     /locus_tag="ACG8M6_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0455741.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(41024..41308)
                     /locus_tag="ACG8M6_000037"
     CDS             complement(41024..41308)
                     /locus_tag="ACG8M6_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0455742.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41660..42370)
                     /locus_tag="ACG8M6_000038"
     CDS             complement(41660..42370)
                     /locus_tag="ACG8M6_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0455743.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42367..43839)
                     /locus_tag="ACG8M6_000039"
     CDS             complement(42367..43839)
                     /locus_tag="ACG8M6_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0455744.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43977..44450
                     /locus_tag="ACG8M6_000040"
     CDS             43977..44450
                     /locus_tag="ACG8M6_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0455745.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44603..45154)
                     /locus_tag="ACG8M6_000041"
     CDS             complement(44603..45154)
                     /locus_tag="ACG8M6_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0455746.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45259..45789
                     /locus_tag="ACG8M6_000042"
     CDS             45259..45789
                     /locus_tag="ACG8M6_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0455747.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46272..46715)
                     /locus_tag="ACG8M6_000043"
     CDS             complement(46272..46715)
                     /locus_tag="ACG8M6_000043"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0455748.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46731..47891)
                     /locus_tag="ACG8M6_000044"
     CDS             complement(46731..47891)
                     /locus_tag="ACG8M6_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0455749.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48398..50098
                     /locus_tag="ACG8M6_000045"
     CDS             48398..50098
                     /locus_tag="ACG8M6_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455750.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(50200..51033)
                     /locus_tag="ACG8M6_000046"
     CDS             complement(50200..51033)
                     /locus_tag="ACG8M6_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0455751.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51337..53502)
                     /locus_tag="ACG8M6_000047"
     CDS             complement(51337..53502)
                     /locus_tag="ACG8M6_000047"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0455752.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53762..56056
                     /locus_tag="ACG8M6_000048"
     CDS             53762..56056
                     /locus_tag="ACG8M6_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0455753.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            56049..56714
                     /gene="pgmB"
                     /locus_tag="ACG8M6_000049"
     CDS             56049..56714
                     /gene="pgmB"
                     /locus_tag="ACG8M6_000049"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0455754.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56707..57714
                     /locus_tag="ACG8M6_000050"
     CDS             56707..57714
                     /locus_tag="ACG8M6_000050"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0455755.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57736..58755
                     /locus_tag="ACG8M6_000051"
     CDS             57736..58755
                     /locus_tag="ACG8M6_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0455756.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(59036..60403)
                     /gene="nhaC"
                     /locus_tag="ACG8M6_000052"
     CDS             complement(59036..60403)
                     /gene="nhaC"
                     /locus_tag="ACG8M6_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0455757.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(60512..61933)
                     /gene="tyrP"
                     /locus_tag="ACG8M6_000053"
     CDS             complement(60512..61933)
                     /gene="tyrP"
                     /locus_tag="ACG8M6_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0455758.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(62176..64053)
                     /gene="tdc"
                     /locus_tag="ACG8M6_000054"
     CDS             complement(62176..64053)
                     /gene="tdc"
                     /locus_tag="ACG8M6_000054"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0455759.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64344..65600)
                     /gene="tyrS"
                     /locus_tag="ACG8M6_000055"
     CDS             complement(64344..65600)
                     /gene="tyrS"
                     /locus_tag="ACG8M6_000055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0455760.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65712..65917)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            66180..67481
                     /locus_tag="ACG8M6_000056"
     CDS             66180..67481
                     /locus_tag="ACG8M6_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0455761.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67550..68149)
                     /locus_tag="ACG8M6_000057"
     CDS             complement(67550..68149)
                     /locus_tag="ACG8M6_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0455762.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            68403..69284
                     /locus_tag="ACG8M6_000058"
     CDS             68403..69284
                     /locus_tag="ACG8M6_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0455763.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            69297..70907
                     /locus_tag="ACG8M6_000059"
     CDS             69297..70907
                     /locus_tag="ACG8M6_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0455764.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(70984..71643)
                     /locus_tag="ACG8M6_000060"
     CDS             complement(70984..71643)
                     /locus_tag="ACG8M6_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0455765.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(71926..72591)
                     /locus_tag="ACG8M6_000061"
     CDS             complement(71926..72591)
                     /locus_tag="ACG8M6_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0455766.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(72738..73343)
                     /gene="dhaL"
                     /locus_tag="ACG8M6_000062"
     CDS             complement(72738..73343)
                     /gene="dhaL"
                     /locus_tag="ACG8M6_000062"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0455767.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(73371..74381)
                     /gene="dhaK"
                     /locus_tag="ACG8M6_000063"
     CDS             complement(73371..74381)
                     /gene="dhaK"
                     /locus_tag="ACG8M6_000063"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0455768.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(74356..74751)
                     /gene="dhaM"
                     /locus_tag="ACG8M6_000064"
     CDS             complement(74356..74751)
                     /gene="dhaM"
                     /locus_tag="ACG8M6_000064"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0455769.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            74905..75225
                     /locus_tag="ACG8M6_000065"
     CDS             74905..75225
                     /locus_tag="ACG8M6_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0455770.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(75337..76194)
                     /locus_tag="ACG8M6_000066"
     CDS             complement(75337..76194)
                     /locus_tag="ACG8M6_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0455771.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(76302..77606)
                     /locus_tag="ACG8M6_000067"
     CDS             complement(76302..77606)
                     /locus_tag="ACG8M6_000067"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0455772.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(77603..78112)
                     /locus_tag="ACG8M6_000068"
     CDS             complement(77603..78112)
                     /locus_tag="ACG8M6_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455773.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(78287..80422)
                     /locus_tag="ACG8M6_000069"
     CDS             complement(78287..80422)
                     /locus_tag="ACG8M6_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0455774.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(80505..82160)
                     /locus_tag="ACG8M6_000070"
     CDS             complement(80505..82160)
                     /locus_tag="ACG8M6_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0455775.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(82162..82476)
                     /locus_tag="ACG8M6_000071"
     CDS             complement(82162..82476)
                     /locus_tag="ACG8M6_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0455776.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            82646..82906
                     /locus_tag="ACG8M6_000072"
     CDS             82646..82906
                     /locus_tag="ACG8M6_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0455777.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            82920..83234
                     /locus_tag="ACG8M6_000073"
     CDS             82920..83234
                     /locus_tag="ACG8M6_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0455778.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(83964..84518)
                     /locus_tag="ACG8M6_000074"
     CDS             complement(83964..84518)
                     /locus_tag="ACG8M6_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0455779.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(84644..86573)
                     /locus_tag="ACG8M6_000075"
                     /pseudo
     CDS             complement(84644..86573)
                     /locus_tag="ACG8M6_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(86879..87595)
                     /locus_tag="ACG8M6_000076"
     CDS             complement(86879..87595)
                     /locus_tag="ACG8M6_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0455780.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(87596..89419)
                     /gene="glpO"
                     /locus_tag="ACG8M6_000077"
     CDS             complement(87596..89419)
                     /gene="glpO"
                     /locus_tag="ACG8M6_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0455781.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(89433..90929)
                     /gene="glpK"
                     /locus_tag="ACG8M6_000078"
     CDS             complement(89433..90929)
                     /gene="glpK"
                     /locus_tag="ACG8M6_000078"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0455782.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(91065..92525)
                     /locus_tag="ACG8M6_000079"
     CDS             complement(91065..92525)
                     /locus_tag="ACG8M6_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0455783.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(92515..93810)
                     /locus_tag="ACG8M6_000080"
     CDS             complement(92515..93810)
                     /locus_tag="ACG8M6_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0455784.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(94142..94819)
                     /locus_tag="ACG8M6_000081"
     CDS             complement(94142..94819)
                     /locus_tag="ACG8M6_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0455785.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(94849..95940)
                     /locus_tag="ACG8M6_000082"
     CDS             complement(94849..95940)
                     /locus_tag="ACG8M6_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0455786.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(96041..96475)
                     /locus_tag="ACG8M6_000083"
     CDS             complement(96041..96475)
                     /locus_tag="ACG8M6_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0455787.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(97059..98516)
                     /locus_tag="ACG8M6_000084"
     CDS             complement(97059..98516)
                     /locus_tag="ACG8M6_000084"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0455788.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(98513..100408)
                     /locus_tag="ACG8M6_000085"
     CDS             complement(98513..100408)
                     /locus_tag="ACG8M6_000085"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0455789.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(100466..102241)
                     /locus_tag="ACG8M6_000086"
     CDS             complement(100466..102241)
                     /locus_tag="ACG8M6_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0455790.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(102291..103787)
                     /locus_tag="ACG8M6_000087"
     CDS             complement(102291..103787)
                     /locus_tag="ACG8M6_000087"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0455791.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(103981..105603)
                     /locus_tag="ACG8M6_000088"
     CDS             complement(103981..105603)
                     /locus_tag="ACG8M6_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0455792.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(105931..107244)
                     /locus_tag="ACG8M6_000089"
     CDS             complement(105931..107244)
                     /locus_tag="ACG8M6_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0455793.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(107489..107713)
                     /locus_tag="ACG8M6_000090"
     CDS             complement(107489..107713)
                     /locus_tag="ACG8M6_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455794.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(107733..108155)
                     /locus_tag="ACG8M6_000091"
     CDS             complement(107733..108155)
                     /locus_tag="ACG8M6_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0455795.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(108297..109007)
                     /locus_tag="ACG8M6_000092"
     CDS             complement(108297..109007)
                     /locus_tag="ACG8M6_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0455796.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(109025..109522)
                     /locus_tag="ACG8M6_000093"
     CDS             complement(109025..109522)
                     /locus_tag="ACG8M6_000093"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0455797.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(109559..110011)
                     /locus_tag="ACG8M6_000094"
     CDS             complement(109559..110011)
                     /locus_tag="ACG8M6_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0455798.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(110146..110751)
                     /locus_tag="ACG8M6_000095"
     CDS             complement(110146..110751)
                     /locus_tag="ACG8M6_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0455799.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(111201..111821)
                     /locus_tag="ACG8M6_000096"
     CDS             complement(111201..111821)
                     /locus_tag="ACG8M6_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0455800.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(111823..112692)
                     /locus_tag="ACG8M6_000097"
     CDS             complement(111823..112692)
                     /locus_tag="ACG8M6_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0455801.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(112715..113122)
                     /locus_tag="ACG8M6_000098"
     CDS             complement(112715..113122)
                     /locus_tag="ACG8M6_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0455802.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(113141..114430)
                     /locus_tag="ACG8M6_000099"
     CDS             complement(113141..114430)
                     /locus_tag="ACG8M6_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0455803.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        33454 a        23674 c        20040 g        37263 t
ORIGIN      
        1 aaataactga ttatttacgt ttgaataatg agaatttttc tatatattct ctattttgat
       61 aatagtattc tcattttcaa aataaataag ggaggtgtga caaaattctt gtcacacctc
      121 ccttttccga ataaactgtg ttccagaaat agcttcttcg aaaataagct gaaatttcac
      181 aaaaatttga gaagcaattt tcgtacattc cttcttcttt cttgaaactg accacttctg
      241 tcacaccctc tcttctatca ctttcctttt ttcacacgac tcccggtttc tttgataaat
      301 ctcgatactt tcaattttct tgaatttttc atttgaggta cacatagata cagacgattt
      361 cttgctctag tcatcgcaac ataaaatagt cttcgttctt cttctatcgt cgttactggt
      421 tctgctttta tttcattaaa gtcgattact ttcttttctg tcgctccgtt ataaggcaaa
      481 cttccttcgt tacaaccaat caaaaagaca tttcggtaac taagaccttt acttttgtga
      541 atgctctgga tacatagtgc atcaagtttt ccttgtctgt aagcaatcaa gcgggttttt
      601 aattctttag ctgtcgcttg ggtttcttgc gataaggctt ggagtcttct gaaagtagca
      661 tgctgcttta cttcttctcg tatccattct tgatcactct ggtcgatttc cttagctata
      721 cgtggcagat gaagatagct ttgaacaagc gaatagattt cttgatgctg gtctgtttta
      781 cgcaacgctt cctcaaactc tcggatttct gcagatttcg attttaaatt ggggcttatc
      841 gacaaaatat aatctgcaac ggctatattt tgatatttag gagaaaagta ttgatcgcat
      901 tcactttttt tcaggtagca gtctgccatc agctgccgga gcaattgctg gtaggcctga
      961 tttttctttt gcagattcag tgctgtttgc caatgaacaa ttttttgata gaaatgttgg
     1021 aaagacttga taccaaagat accttcatat ggactatctg cttccagtac aaaaggaata
     1081 ttcgttttca gcagagattg gacaagctgc tgcgtttgtg aagctgatcg gtataatatg
     1141 attgattcct tatatttccc atttttttga ataaatgtct gaatttgttt gattagccac
     1201 tgactttctg ctttagtatc agaaaacaaa taggcttcca taacacctgc ttctttttgt
     1261 gctggaatca tcgcctttgg tatccgatca tttttattat agctgatcaa tctatttgct
     1321 ccttggacaa tagctgcccg agaacgataa ttagttaata agaaaagagt tttggtagga
     1381 tattcttttt caaagttctt gatgtatgct ggctctgatc ctctgaatcc atagatactc
     1441 tgatcatcat ctccaacaac aatcaacttg atcctgtttt cctttgtcat ccgtttcacg
     1501 atttcccatt gaagtggatt agtatcttga aattcatcaa tgaatagata atcatatttt
     1561 tggccgatat aatgctggat ctcctcattc tccaaagcgc ttttcagatt ttggagtaca
     1621 tcattgaact ctagcaattg ctggcttctt ttttgttctt ggagttccgt gagcaatcca
     1681 tacagtcgtt ctgatatcgg ctgtcgtgca ttcttatcaa tggaatcata tttttcttta
     1741 actaactgaa ccatttcttc tactgaaagg ttcatattct gccaatagtc taaccgagaa
     1801 gcaatcgttt tttctccata ttgatcaaat atataaagat tatatttacg tatcagctgt
     1861 tgcaaaacag cattcacttt ttgtgcagta tcctttgaaa agtctccttg tattccttgc
     1921 attgctactt caggaaattt tcgacaaatt tcattgtata tcctaaaaaa gatactatgg
     1981 aatgttccga tatggacagc ggtttttctc tggctgaaat tatctactcg tttttttagt
     2041 tcatttgcag cgttccttgt aaaagtgatc attagtatgc gttcttcgtc tacttgtttt
     2101 tgcgtcatca gataaacagc ttttgcacag attgtttgcg ttttgccgct cccagctcct
     2161 gctatgattc gaagatgttt cttcatatca tatgtgatag ctgctttttg ttcttgattg
     2221 aagcgattat aggaacgttt ctccatcatt ttaaagaaag agctgtttcc ttcactttct
     2281 tttacttgta cttcttcctc aataagtccg ggtgcttcgc cataagccgc ttcataggta
     2341 gcaatgattt cttcttgtag ccgaaagact tgttgaagct ttttatactt gttctcccag
     2401 cgtttcaatt ctttttgtaa tcggagaatt tctttttcat tcaactgatt gatttccttc
     2461 ccatctttct tctttgaatt tgtttatttc aagttggtta tttaaagttt cgttgtctat
     2521 tttttcttta tactaacaga cctttctaaa aatgaaaatg ggaagcgata tgattttagt
     2581 tttctgaaaa tttttccttt ttttcaatcg tttatgctaa gataaggaag acaaaaatat
     2641 tcataaaagg tgatgaaaaa aatgcagact tcctttcacg aggggcgtac aataggactt
     2701 ttgctgacat ttatcggagg agccatggac agttatactt atatccaata tggtgctttt
     2761 gcctctgctc aaactggcaa tatcattcta tcgatcatcc aagcatttga cggagaatgg
     2821 ctaagtgttg gtaaaaaagt attgtctacc cttttctttt tcttagggat tcttctaaca
     2881 aagtatctaa ttgattattt tagaaaaaaa gaaaaacatt tctggcgctt gtttgttcta
     2941 tattatgaag cagtgatttt tttctttgtt agtttgacac ccgtccatga gcatcccgca
     3001 ttagtaacga ttttgattgc ttttactgca tcgatccagt gggtagcttt tgataaaatc
     3061 aacgggcgtg cctacactaa tttatttaca actggaaatt taaaaggggt agcaacgaac
     3121 ttatatgatt attgtatttc taaagaaaaa gcagattttg atcgattttt ccactatcta
     3181 agtgtcgttc ttgcctttat cgcaggagct attgtccttg ttttctgtca tcatctgttt
     3241 ggcgcccatt cgattttgat cgtttccatt ttatttttga tcatggccct cactcagaca
     3301 tttcatttat ggcaatttta tcatacgaat cggttcacta tttgatctca atgaatcaaa
     3361 aaaactaagc aagatgctgc tcacagagaa atatctgcga gtgagcaatt tgcttagttt
     3421 ttatattatt tagttgtaaa gattcccttt gctttcgggt tatttgtagt gtagcggatc
     3481 gactgggcat ctgcctcttc tgtcattttc tttgcccgca tcttgatttc ttgtacgatt
     3541 tgttctccac atgccggatc ggctacgaaa tcatatttgt ccccatcgat cactattttg
     3601 ggacatatat caaactgttc ataccactct tcgtatcgtc gattgagttc tttatagtag
     3661 gaatagagtt caggatcttg ttctagctgt tcatattccc ggcctctttt tttgattctc
     3721 gctaacatag tttcaaagga tacacgaata taaaccagta gatctggttt cttttgtggc
     3781 gaaatttctt ctaactcctt cagcatcgtt tctaaaagac cttcatactg ctttacttct
     3841 acttcagaaa cacgccctaa atctgcattc aaatgaaaca gcatactatc ttcgtagatg
     3901 gagcggtcta gtacgttgtc atcatggctg aaagcatctt tgattgccaa aaagcgtttg
     3961 ttcaaaaaga aaatctgtaa taaaaaagta tacttttctg gattagaata aaatagcggc
     4021 aatacttcat tatcttccac gttctcataa aaaggttttg tttctaaatc ttgggcaagc
     4081 atttcagtta gtgtgctttt ccctgcaccg atcgttcctg caacaatgat cacaattatc
     4141 ggcatccttc ctgtaataaa atctgttctt cattttacta cttcatttat agaaataaaa
     4201 gccttgactt tatctgctcc ttaaaagcta cttctctaaa agtagccaaa agtaactcct
     4261 attcatgaaa ataaaggctg tgacaaaagt cttgtcgcag cccttttttc aaataaacag
     4321 tattctcctg ccaacacctc ggtcacaagg caatattcat aaaacatgaa aagctgtttt
     4381 ttaacattat tccttgtaac tcagagctaa acgggagact cacaacctct ttatgctctc
     4441 aacattttat ctgtttttat tcttctattg tacttgattt gcgtacaact aactgccagt
     4501 gcagattatg agcggtatca ttgtcttcta ctgcgcattt catgacacgt atcatcacac
     4561 tattttcata gattttttct atttttcctt ttattggtga ttcaaataaa tcagatttaa
     4621 atgcatatgt ttcgccgact tcaaacaatt gttcattttc cataagaatc cctccatcac
     4681 cttttataag gtcagtaatt ttttaacata aatcactcga aaaagcacaa acttgtcata
     4741 aaaagtactt ttcgtatcaa aactgtcact ttttttggta aggttcttgg aaaacgaaga
     4801 tttttttagg agatcatgac ggttttgatg tcaaaaccac tttttatgaa acaaaagacg
     4861 ttttttattt tttttagcat aaaatttgtg gtggaggtac gtatgagatg aaagataaag
     4921 ttgtgaacaa attattagaa gccttattca gcggagacaa atcggaagaa gatatggtgt
     4981 atgtccaagt aaaatttgtc ttagaagttt tattgaataa tcttggaaaa ctagcggtcg
     5041 ttctagtctt ttctctggtg acaggctcat gggcggaaac aggaatcacc tttctttctt
     5101 atatctgcat ccgcagatat gcttatggat tgcattccga ctctgaattc gtttgtctgc
     5161 tgtggacact actttattta tggggcgttc ccttggtgat gaaacatctg caattgacta
     5221 tctccttccc aatgatggtc tttttgttaa tcagctgctt tttgttactg cttcgctatg
     5281 gatcaagagg tacagcaatc aatcccattg aacccgagaa acgtccccca ttattaaaga
     5341 aggcgatcag catgtttcta attttttctc tcattacgtt atttttctca gcttcttact
     5401 tttctaccta tttgctactt gggattgttt tagaaatagc tacgttgctt cctattacaa
     5461 actacttgat gaattttgga ggaatctttc atgaaaaaaa caatcattaa acaattaact
     5521 aacttatctg acttttttgt atctggctgc tgctttggta tgagttctga attcaaaatc
     5581 cctgaaaaat taaaaaaata atacgccaat tgtgctataa tttggtaaaa atcaatggga
     5641 gctgtacttt atgtttttat ggtccatagc gttgcaatat ttgatttaca caatttgtat
     5701 cttattgatt gaccatcaca tccgatacaa atatctgttt gcgggagtca ttctgatttc
     5761 ttgtattttg attggatggg tctattcgat tttagggatt tttactgctc taattgctat
     5821 aggaatgctg atgcttactt ttttcttctt ttcgacttcg cgtcaacaat tggtttacgc
     5881 gcttcctttt gggttattga cgtcgctttt aggagatcac ttaacaagca tcggcgattt
     5941 ttttgttttg aaatcttcgg ttattgaacc aggagattca ttgcagtatt ttcacattgt
     6001 cttatccgct tttttatctg ctttattagc tttttgttta aaaaaatcgt tagagacttg
     6061 gtttcacact gtggaccgcc gtatgatcgg tacgatcggg acattgatat tatttacata
     6121 ctatatcatt attttttata cccgtttttc tggtgaaaca cctgatgtct tagctttgaa
     6181 tacgagtttt ttcattctat atctgtgtgt tggcctgatc atcttggttt attattggaa
     6241 aatctctaat aagaaattag ctgatcagtt attagagcaa caagtcagga tgcaacagga
     6301 ttatatcaaa gatctagaaa aaaactatca agacttgaga gaattcaaac atgattacca
     6361 aaatctgctt ttttctcttc acagctatat ttcagaaggc gacttagaag gattacggtc
     6421 ttattatgat caaacgatat tacctacacg ccagatgatg aacctttttc cggccaatct
     6481 aagtatgttg gataatatga aagttcctga gatccgtagt ttactgtcat taaaactcat
     6541 gatggcacag gaaaaagggt tgatcgtcca gcttcatttt cctgagccaa tcgaactgga
     6601 tacgaaacat acggtaaacc ttgtacgtat gcttggtatt atcttggaca atgcgattga
     6661 aggagctagt gctgcaaaga agaaaaaaat cgaattgacc atactcaaga agaatcattc
     6721 cgtgatgatt cgtgtgatca atacaacaac aaatacatta ccgttaaacc agttgaaaca
     6781 aaaagggttc tcaacaaaaa caaatccaaa aaacgaaggc cttggcttgt ttatcttgga
     6841 tgaattgaca aagaccaatc cttatctttt ccttgaaaca tcaattgaaa accagcgttt
     6901 ttctcaaacg atttatatac aaataaaata aaggagtaat gatatgattc ccatttatat
     6961 ttgtgaagac gatccaaaac aattagaagt tttatcaacg gtgatcaaaa atagaatcat
     7021 gattgaaaac ttagacagtt atgtccatat ttctacctct gatccggaag aacttctcaa
     7081 tgcagccaag atcagaactt cttcttttgg tatttacttt ttagatatcg aactagaaga
     7141 cagtgaaatg gatggtgtag cgatcggtaa attgatccgt gaattagatc ctttaggaaa
     7201 aataatctat gtgacttccc atacggaaat gtcgatgaaa atcttaaaaa gcaacatcga
     7261 accgactgat tatatcgtca aagaagatct ttatgattta aaagaaaatg tcgagcatat
     7321 cctgagcaaa attttcgaag attttcaaat gtcccaaatc caaaaagata tcttcaaaat
     7381 agagtttaat gatgagatca aatttttacc gatcaaagac atccaatatt tttctacttc
     7441 gctaggagcg ccacataaac tggaagtcca tttgactcat gcccaattac aattctatga
     7501 gaagatcaaa gaaatcgaga aaatgcatcg gaattttatc cgttgtcata aatcctatgt
     7561 tatcaataca caaaatgtgc ggtcgattaa taaaaaaacc agagaagtca cgttggcaaa
     7621 cggcgagacc atcccagcct ctatccgtgg attaaagaaa ttgatttcat aaaaagaacc
     7681 gaatagtttg gaccgactaa atgaccagcc gatccaaacc cttcggttct tttttgccta
     7741 tcagtgcaaa agcatttcct gtgcaatttc acgtccatcc attgtatctg gataaaatgt
     7801 tggccaattc tccatttctt tcagcaatac ttcttgactt tcatttcctg catataaatg
     7861 aaaatgttcg gagtcagccg gtgcaatcgt gtgatcactg aattgaatat atctaaaagc
     7921 gccactttcc ttatcttctg cttcgaatag ataccttacg ccacggtttc ctgcctcgta
     7981 ggtcaagatt tctttcccta cataacgata atttgaacgt ttcgttgttc cattcttaaa
     8041 cgtaaatgcc atctctttct cactgatctt gattgaagca atatctgttt gatagccctt
     8101 cgtataatat tctttatatt cttgtgctgt cttatcctga ttcaattttt ctttatattc
     8161 aaaaacagga tcaaaagtcc catcttctac atacgtatct actgtttgcc aagttcctgc
     8221 ccaatctgaa agagagcgat cttgtacatc ctcgtcttca aaataaccgt tgtagacgct
     8281 cttttctttt ttgggggtca aagtatcttc tagcgggttt tctgtctctg tcgttttttt
     8341 caacgcttcg aggtttgctt ccatgataga aagatagttt tctccatttt ctatttgttc
     8401 attcgtcaat ccttccaaag gattcaatac ttctaaagat acacctgctt cgtttgctaa
     8461 cgttctggca atactatctt tggcgttttc ttcaaaataa atatagttga ttccatgttc
     8521 tttaacaaag tgtttcagtt cagcaattct cgccgcagac ggttcttcac tagaagaaag
     8581 accagcaatc gatacttgat tcaatccata ttctaacgct agatagttga atgcagcatg
     8641 ttgcgtgaca aaagatcttt gttttgcttg cttaaaggtt tcttgataaa gctgatggag
     8701 tgcttgaagt ttctcggtat aggctgctgc attttttgtc catacttcct gtttttctgg
     8761 gtaagcttca atcagctgat cacgtatgtt tgctacctgc ttgattgcta aacttggagc
     8821 tagccataca tgtgggtcta gctcatgtgt atggccttct tccccgtgtt catggtcatg
     8881 atcatgtcct tcttcacttc ccggaagtaa aagcatacct tctgtcgctt ttatcgtatg
     8941 gacattgcct tcttgtaatg ttttttggat tgcggggacc catgtttcca tgttttcatc
     9001 attataaaca aagatatctg tatcttggat ctttgccatg tctttagcag aaggttcata
     9061 atcgtgggaa tcagtccctg ctggaatcaa taactctact tctccttcat ccccgacgat
     9121 tgcttttgta aaatcataca tgggataaaa ggtcgtcatc acgctcaatt tttctttact
     9181 gtcctcggct ttcccacttg ccccgcaagc agttaacata cctgctaaaa ccagcatggc
     9241 caatatacct atcaatcgtt tcatttatga cactcctctt ctcatcttct ttcatttgaa
     9301 tgttccgatt cgcttttcgc aaaccgtaat agttacgttt tagcaacagg atttaattta
     9361 tcatatggtt aaaagaactg tcaagcgttt tcttatttta attgatgaga aagtttatat
     9421 gaatgattga cgatattttt gcaatcaacc tctatcattt tccttttata cccgcttttg
     9481 tctataatga atttatagat aaaggaggaa aaagagtgaa aaaagaaaca gatacaacga
     9541 atcgtttgat ccgcttttta ggaggaaaaa cctcttacta tgttttagga atagttatct
     9601 tacttgcgct gatcatttat tttttccatc agatctcgtt tatattcaag cctgtcggaa
     9661 tcattatcgc tactatcttg cccccattat tgtttgcact gatcttgtat tatcttttgg
     9721 aaccagtcat caaacgtttg agcaagcacc tttcgaggaa cttgtcagtc attatcgttt
     9781 atgtgattat tttattttta ttggggcttg gaagcatatg gcttattcct tttcttgaag
     9841 aacaggcaaa gacattgatc agtagtttgc cagatttgtt tcaagatttt caagagagtc
     9901 tccgacaatt tttggagaaa acaccttttg ccgattcatt taatcaattt ttcgcttcta
     9961 ttgacgacgt gacgtctgat atagctggat ttattggaaa ttattgggaa agtggcgcac
    10021 aacggatcag caacattttt tcaactgtta cagctatttt tattacttta tttactggtc
    10081 caattgtcgc ttttttcctt ttgagagatc ctcaaaaatt ttatcagtca gttttagcga
    10141 ttgtcccacc agcatttcgt acagatttta aaaatttgac aaagattgct aatcaacaat
    10201 tgggttcatt tctaaagggg caaatcattg cttcatttat ccttggagca gtctactggg
    10261 tttgtttctt actgatcgga ttagaatttg ctagtgtttt agctatttct gctgggttgt
    10321 tatgtatcat tccatatatt gggccgttta tcgccttctt tccaggtttg atcattgcat
    10381 tccaagactc tacttttatg gctgtcaaat ttgtcattgt ctggtttgcc gtacaacttc
    10441 tccacggtga cttggtgatt cctcgagtaa tgggtgatcg tttacagatc catccgatta
    10501 cgatcttaat cgttttactt gtcatgggtg atctaatggg aattgtgggc gttatttttg
    10561 gtattcctat ctatacgtta gtcaaattac tcgttacttt tattttccgt aaattcaaac
    10621 aacgctacaa taaattttat ggcgataaag gagagtatga aaactcttca ttttctgaag
    10681 aagattattt taaataaaac acaaaacgtc gatcttacga gaatagctcg taggaacgac
    10741 gttttgtgtt tttgttttaa tttttcagca aagcaattgc ctggtatggt tttagctcca
    10801 gactttgatg aagaacagta tcggaataat tatcgataag aatattggct tctgtgaatc
    10861 cttctgggat ggcgatcctt gtatctttat catagaaatt tgttaataca agcagctgct
    10921 gtccttcaaa ttggcgaaga aagccataaa cttccggatg atttgcttca taagcatgat
    10981 aatctccttg tgcaacgatt ggatatgttt ttctcagccg aatcaatttc ttgtaatagg
    11041 acaaaataga atcttcagaa gatagttctt tttctacatt aatctcttct agcttgcctc
    11101 caacttttaa ccaaggtttc cctgtagtaa atcctgcttg ttctgtgtct gaccattgca
    11161 tgggtgttcg agagttatcg cgagattttg ctttgatccg acgaaacgct tctttttctg
    11221 tgaatccttg ttcaaggagt aacttataag cattcaaact ttctacgtca atatagtcgg
    11281 aaatcgatga atagtctggg tcgatcatgc cgatttcttc tcccatataa atatagggcg
    11341 tcccacggtt caaatggatc atggttgcta gcatctctgc tccttgtttt cgatactgat
    11401 cgatagctac aaatcgattg agcgcacgtg gctgatcatg attattaaaa aatagagcat
    11461 tccagccgtt accctcactc atcttttctc cccatgtatg aaatagtcgc tttaattctg
    11521 caaaatcaaa tggaggagta gaccatttat tcccattttc ataatcgact ttcaaatgat
    11581 ggaaattaaa gaccattgat aattcttctc tactaggttc tgtatacaag atacaatttt
    11641 cgatcgtcgt agagctcatt tcaccgactg tgatgatagt ggaatccgtc ccaaacgttg
    11701 cttggttcaa ttcttttaga tacgtatggg tgattggttt gtccgtatat tcaggtttgc
    11761 catcattttc tttattattt ttcaattgtt cgtctttacc gataacattg ataacatcga
    11821 aacggaatcc ttttacccct ttttccatcc aaaaacgaac aactttgaat agttcctctc
    11881 ttactgcttg gttccgccag ttcaagtcag cttgtgtccg atcatataag tgcaaataat
    11941 atttccccgt atctccaaat ggcgaccaag cacttccacc aaattttgat acccaatccg
    12001 ttggctcttc tcttaaaatg aagaagtcct gataatgctg gtcccctgct agtgcttttt
    12061 tgaaccattc atgttctgtc gaaacgtgat tcagcaccat atcgagcatg atttctatcc
    12121 ggtatttctt tgcttcggct attagctgtt cgaattcctc catcgtacca aagataggat
    12181 cgatggctgt gtaatcagag atatcataac cgttgtcttt ttgagggctt ggataaatag
    12241 ggtttagcca aatcatgtcg atgcctagat cattcagata cgggagtttt tctttgattc
    12301 cttttaaatc tccaattcca tcaccattac tgtctttgta agatttggga tagatttggt
    12361 aaatgacttt gtctttaaat gtcatggata tttacctcca ttcaactgat ttattaccta
    12421 ccacaactta tatatacaag ttatcattta ttattataac tgaaccgaaa gcgtttgtaa
    12481 aatttatctt tatacattct aagaggtctg ttgcccaata aaaaacaaga gatcagacat
    12541 gtatttgtct atcctcttgt ttaaaatagc aaaatattct tgtttcttct ataaatggtg
    12601 ttcaaaagct tttatctacg gcaatcagtc ttctcaaatg taaaacatga ggagctaatt
    12661 ttatttgagt attctttttg ctcgatgcag gacggctttt ttacaacctc tgattcatgg
    12721 tgttcaatca gctgtccttc ggtattagct caaaaaacgg caaaatatga aaaactattt
    12781 tgccgtttgt ttctctaata ctcaggtcaa accgctgatt tcacaacctc tgtttttatt
    12841 cattcgattt caacgcatca atcatatcga tatgctttaa tttgaaatgg gtaacaatca
    12901 tcacaataac cgtaaatacg atagttaatc ctgctgataa gacataagcc caccatgtga
    12961 tcactaatgg aaagatgaca ttctccatag acgcttgttg caagatgaag tcagtcaata
    13021 tatatcctac accaaatcct ccgatgattc ctaaaagagt aaagatgata ttttctcgaa
    13081 caatgtacat cgtgacttct ttatcaaaaa atccaaggac ttttattgta gataattcgc
    13141 gtacacgctc ggaaatattg atattagtta gattatacaa gacgataaaa gccagcagtc
    13201 ctgataatac gacaaagatt aagacaaccg agtctaagtt cgccattgat tcttcttgcg
    13261 tttcgatttg tttggacatg aaagacgtat ttgtcacagc gtctgtggca agcagtttct
    13321 cggaaagcat ttcttcttct tttgttgtca tcttatctgt acgcagcaaa aatgcattat
    13381 ccatcatcga ttgatcagta actgaagcat aatattcagg ggacatataa ataaagctac
    13441 ctagatagtt tttcacgatt tcttttactt cgactttcca ttctttttga tcttcatcat
    13501 aaagcgaaat atgatctcct gtagataagt caaatagttc tgctactttt tgcgtaatag
    13561 cgattcctgt tttaggcaag gagcgtgggt tctcttcaag atcagttaaa tggataaaac
    13621 gatccaaatc agctgtttta cttggtacga tcactgtcag actttgcgaa gcatgtcctt
    13681 tttttctcaa ttctacggtt tgagtatgaa tagctaggat ctcatttact ttttcttgct
    13741 tctgcagcat atccgctgct ttttgacgac tatcttccgt ggatgaatcc aatgtaacga
    13801 ttgcttgata gtcaatgatc ggtccaaatt gtttggctga aacagaactt agagaatctt
    13861 tcaatcccac gcctgcaacc atcagcccgg cacatccagc aattccgata atcgccataa
    13921 gcattcttga cttatatcgg aagagatttc ggtaactgat tttttgatta aagctaaggc
    13981 gtgaccacag cggagtgatg tattcaagaa aaatgcgttt ccctggcttc ggtgctttcg
    14041 gttgaagcaa tgcagcaggc cgttctctta aatctttgaa taaaacgatc attgcagcgc
    14101 caaatgcggc aatgaagaat gcggcagctg cttgtaaaat cggcggccag tcataaaaaa
    14161 taagcgcact cccaatatca taccgctcgt tagacagctc aaaaatgatc cttggcaaaa
    14221 gattcgttcc taatatcgtt cctaaaacaa tacctatccc tgaggctaga cttgcataga
    14281 ttgcatattt ttttgcgatt tcaactttcg tataacctaa cgcctttaat gtcccgattt
    14341 ccctgcggtt ttcttctacc atacgtgtca ttgtcgtaaa ggtgatcaat gcggcaataa
    14401 agaaaaagaa aacaggaaaa acattggcaa tcgcagcgat acgatcagac agatcaccaa
    14461 attcttgaaa accgacattt tctgaacgtt cattgtatag gtatgatggt ttagttaaat
    14521 tttcaatttt ttctgcattt cgattcaatt cttcctctgc agaggccagt tcacgttctt
    14581 tctcaactaa ttgcctttct tgttcagcaa gttgagcttc ttgttgagat aatgtttgaa
    14641 cagcgattgt tcgttgcgct tcaggaagct gctggatagt tgctgactgt tgcgctaact
    14701 gttttttagc atcttctaac cgtgttcttg cttgcgttgt ttgtgctttg ccatccgcta
    14761 ttttttgctg tgccggttct aattcttcgt tggccgattt tttcagctct tctaagcgtt
    14821 cttcgggtcg atcttgtaaa tacttttctg ccttttcttg attttgctcc attttatctt
    14881 tgtaagcttt gctgtacgta ttcaaattct ttacgtttgt aaaatcaaga tataagacag
    14941 aataaatatc tgttttaaaa acggattcag gtaaatagac aaaataatcg acgctaccat
    15001 ttccgacgtt cgcgtatcct ctctccaaat tgttgatgaa caatggagaa cggacaaagc
    15061 ccgtgatggt atactctttt tctttcaatt catctgaatc aatctgataa atatcaccga
    15121 ttttataccc ttcatcttcc gctttttcat ccaatacaag ctggtttggt ttttccggga
    15181 gttttccatc agttaattgt agcttgttct gcttgttatt tttgttataa gaaaatattt
    15241 gaatcacttc gtttttgcca gtattggaat aatagatctg ttttcctgtt tctacttttg
    15301 catctgggat cttttctgct tgagcaatat ctttttgtgt caagccacca gtagaaacga
    15361 tttgcaaatc agaaagtcct gctgtaccaa catagtgatc catcgttttt tgcatgacag
    15421 gtccagcagt tttgacacct acaaataaaa gtgttcctag catgacaata aggatgattg
    15481 caatgaatcg tcctttagat tgccgaattt cgcgaaaacc tgctttaaga tatgttcttg
    15541 ttttcataaa atccccctac catacaatct catcaatgga tgaaggatga tcatttaatt
    15601 caacagacct tactttagca tcattaatgt gaatgactct atcagcgatg ggcgctaaag
    15661 cagaattatg tgtaatgatc aaaacggtat tgccgtgttg tctgctagcg ttttgcaata
    15721 atttcaatac ttgcttgccc gtttcaaaat ccagcgcgcc tgtcggttca tcacataaaa
    15781 gaagtttggg atttttagct agtgcacgag caattgaaac cctctgctgt tcgcctcctg
    15841 ataattggga cggaaagtta ttcaatcgat gagctaggcc cacttgcctc aatacctcta
    15901 caggatcaag cgcatcagga gaaacttccg ttgccaattc cacattttct tttgctgtta
    15961 gattaggaac gagattgtag aattggaaaa cgaaccctac atctgttcgt cggtaagcag
    16021 taagctgttt atctgaaaat tgcgcaatat ctgtgtcatc aataatgatc tgaccttcat
    16081 caggtgtatc catcccacct aaaatattaa gaatcgttga ctttcctgct ccacttggac
    16141 caagtataat cactaattcg cctttttcaa ttgaaaaaga aatatcgtca tttgctgtaa
    16201 tggtcgtttc tcccattttg tatcgtttgt attcattttt tacttcgaca tacttcactc
    16261 tgattcctcc attttcacga tatttctcgc tatatctatg catcgaatgc gttttctcac
    16321 acttatttat tttaccactg aatttctcta gactaaagaa aggaatcctt taatttagtt
    16381 gacaaataaa cgatttatga tttacttaca taaaaaacat tgttatttga aagaaggaac
    16441 atcatgaatg atttaaagga aaatatgatt tttgatttat caaaaactgc ttttgtcgcg
    16501 atcgacatgc aaaaagagat cgtaaataac ggtacactct ctccacacac tgcagcatct
    16561 attctgacaa acaatgattt gttagtaaga acattgaaac gtaccgctgc cttgaaagtt
    16621 ttagtgaacg tagacatctc cactttccct tatttgagtc aacaaactga tatgggtgga
    16681 atgactgctt ctgtacctcc agaattcact gatttacttt tgaaggattc tttgaaagat
    16741 acagataata tgttgactat cacaaaatac aatccaagtg ctttttttgg tacttctctt
    16801 gatcttcaac ttcgccggcg tggaatcgaa actatcattt taagcggtgt agcgacaaca
    16861 aacggtgtat atgctactgc attagatgcc tttcaacacg ggtaccatat cgtgcttgct
    16921 gaagatgctt gcagtgatcg tgacaaagaa tcccatcaat tgttcataaa aaagatattt
    16981 ccgaaaactg cacgagtacg ctcaacgaaa caaataatcg aagctatcca acaaagcaaa
    17041 taagtactgg cacacgtgat tttcatttac ttctttttga tttcatggga aaataaccac
    17101 aggatttaga taggagtatg aacatggtga aaaaaaagat cacaatcaaa gaagtcgcta
    17161 aactttccgg cgtttcaatc acaactgtct ccttgatatt aaatggaaat acggagaaat
    17221 tcagtccgaa aactgttcag aaagttttag ctgccaaaga agaattaggc tatcagcctg
    17281 actattttgc tagacaaatg attaccaagg aaacgaaaac aattggtgta ctcgttcctg
    17341 atatcacgaa tccattcttc agtacactga tgcgaggaat tgaggatatc ttgtataagc
    17401 agaatttcgt cacgatatta tgtaatgctg attctgatca tcaaaaagaa atcgaatatc
    17461 tagccgagct cacacgtcga ggtgtggatg gattcattat cgcgacttct gctgtctcta
    17521 cagatgcaat caacgaaaat ttgaaaaaac aaggccggcc cttcattgtt ttggatcaga
    17581 aaaaatctga aggatttagt gatgccgtac gaacagacga ttttcgcggt ggttacctag
    17641 cgggcatgca cttgcttagt ctaggacatc aaacgatcgc ccttgtctat cctgaaaatc
    17701 caccggaaaa tgtccacgct cggattgagg ggtttaaatc tgctcttgat gtttaccaaa
    17761 tccctcacga ccaactgatc ctcttaccta cacagttctc aaaacaaggt ggctaccaga
    17821 tcacagcaga acttcttgat tctgctgcaa ctggtgtgtt tgctttaaat gatgaattag
    17881 cttttggatt atatcgcgga ttagaggaag ctggaaaaag catccctgaa gactacagca
    17941 ttattggcta tgataatata gatatgtgcg agtatatcaa gccgaaactg acaacgatcg
    18001 cacaaccaat cttcgaattg ggacaaacat ctgccaagtt attgttagat cggatccaat
    18061 tcccagaaaa agaatgggaa gaaaaaagat tacctgtacg attagaaaaa agattttcaa
    18121 ctgcaccctt gaaatagctt tcaaggtgtg ctatattttt atcgaactat taaaacgttt
    18181 tactaaaacg ttttaaaaaa taattttccc atcaaaataa atgagatagt tggaggtttg
    18241 taaaatgaac acggttacag taattggaag tattaatatg gacactaccc ttcgtttaac
    18301 caacatgccc aagccaggcg aaacgatgca tgcgcatgag atttttcacg ctggcggagg
    18361 taaaggtgcc aaccaagcag tggcagccaa acgttcaggc gcacggacat cttttattgg
    18421 aggcgttggg gctgacagcg aaggacaaca gttattagat ctactgacaa aagaaaacat
    18481 cgatacatct gggatagctg aaatccaagg cgccacaacc ggacaagcaa tgattatggt
    18541 agacgcagca ggagaaaata gtatcttgat ccatgcaggg gcaaataatg ctttccatga
    18601 acaagaagtc ctaaaaaaca agcaactgat tacaaacagt gactttatta ttgctcagtt
    18661 tgaaagtagc ttagatgcga cgatcctagc tttttctatt gcaaaagacg caggaaaaac
    18721 aacgatcctg aatcctgcac cagctcgtga aacgattcct actgaactac ttgaaaagac
    18781 agatatcatt ataccaaatg aaacagaaac agaaatcatt acaggaatca gagtgacaga
    18841 tcataacagt ttggtcgcag cagccgaaaa actccatgaa ttaggtattg gaacagtaat
    18901 cattacacta ggcagtgccg gcgctttcta tcatacagaa aaagaacacg gtatcgtccc
    18961 tgcttttaaa gtggatgccg ttgacacaac agccgcaggt gatacattta ttggcgcatt
    19021 aagcagcaca cttcagccag accttagtaa cttgaaagaa gcgatccttt atggaaatct
    19081 agcctcttct gttgctgtgc agtcttatgg tgcgcaacct tctatccctt accgcgaagc
    19141 tttagaacaa tcagcagtct aaaattaaca atgagaggaa gaaacaaaca tgaaaaaagg
    19201 aaaagtaatc aattcagata tttcacgagt aatcgcacaa atggggcact ttgataaatt
    19261 aagtatcggg gatgcaggca tgcctgtccc ctttggtact gaaaaaatag atttggctgt
    19321 ctcaaacggt cttccaagtt ttttatctgt tttagaaaac gttttagaag aacttgaggt
    19381 acaatcgatt gaattggcgg aagaaataaa gacaatgaac ccggaaatat tggaaaaaat
    19441 caaattattg ttaccagata caccgattag ctttatccca catgaagaaa tgaaaaaaga
    19501 gttgaataac tgtcatgcat ttattcgtac aggcgaaatg acaccttacg ccaatattat
    19561 attagtgagt ggagtggtct tctaatggaa acgacaatga atcctgaaca gatggtactg
    19621 tccgagcaga ttcatatgct gattgaacag ttgggaggaa aagacaatat tgattctgtc
    19681 cttccatgtg cgactcggct tcgtatcaaa ttgaaaaaac agccagtagc caatgcagaa
    19741 aaattaaaac agcaaatccc ttccattaag tatttcattt tactcgatag atatgcagaa
    19801 ataatcatgg atcagggatt ggctcctgct tacaaggaat tgacgcgtca aatcgatcat
    19861 tcagctccga aagaaaagct aaatccgatc gaaaaactat ttggtttgat atcggaaatc
    19921 tttacgccga tcattccgct gtttgccggt tctggtatat taaaaggtct ggttgtttta
    19981 gcgactacga ttggctggct tagtgaaaca agcggaactt atcatatttt gtctgctgcg
    20041 tcgaatgccg tcttttattt tctgccaatc tttcttgctt tttctgctgc caaaacgttt
    20101 aaagtgaatg gatatattgc ggctgtcatt gctgcagcac tgattgaacc gaatctgacc
    20161 tctttacttt cagacagtgg caaagcagct actgattttc tggggattcc tgtagtactg
    20221 atgcagtata cgtctactgt catgccggcg attttaggta ttttctttta ttcttttgtt
    20281 gaaaaattcc tcaaaaaata tattcgcgaa aatatgcaat tagtatttgt acctttactt
    20341 tcattagtta tcacggttcc tttgacgtta atggtattcg ggcctgcggg tgtttacctt
    20401 ggcgaaggtt tagctgccgc aattacttgg atgatggata ttaacggacc gatcagtggc
    20461 gcaatcatcg ctggtggttg gaatattttg gtaatatttg gtatccaatg ggctgtaaac
    20521 cctgtcatga tcagtaatat ctctgcttat gggttcgacc gaatcgttcc gttgacagga
    20581 gcagccaatt ttggaatggc tggtgcagct ttaggtgtat ttctgcgaag taaacgttca
    20641 aaaacccgtt cgatctccgg ttctgctttt gcttcgattt tacttgcggg agtgaccgaa
    20701 ccaactgttt acggaattgc gatcccattg aaaaaaccat ttgttgctgc ttgcattggt
    20761 gcagctgctg gtggagctgt aatgggattt gcccaagtga aagcgatcgc ttttgtattt
    20821 ggtagtctga ccacattgcc tgcattcatt tccggtactt ttttctggta cttagcagga
    20881 ctggctgtta gtctagtcgt agcaatgatc actactctcg ttagcgggtt tgatgaagac
    20941 ctaatgtctt atgaatagtt aatgaaaaag actgaagtat gagatgttta tcacatattt
    21001 cagtcttttt ctttttagga tcattatttt tataaatttt tgtgatactt tccaaacttt
    21061 ggtataattt acatatgcat aaccattttt atccccactt gtttatcccc aaacaaaggt
    21121 ggtctatgca ataaaatgaa cgtcaaagaa aaacgaatga atgctcgttg cttatttaca
    21181 aattgtttta tttagaatac acagacagat ttatgaaaag aaaaagcttt tcgacacgat
    21241 tatctgttcc aaaaaatgtc taatgaatgc tgaataagac agaaaatcaa tgaaaggaaa
    21301 aggactagtt tattatgaaa aataaaaaaa cgatacgttt gctagctgct acttggctga
    21361 ttgctccgtc tgttttgtcg accaataccg tatttgccgt agataatcaa atagctgatc
    21421 aaacagtcac aacagatacg actacatcca gctcagctac tagtgaaaca acggattctt
    21481 ttacagaaga cccaactgga acaactaact caacaacagg atcgaccgag ccaactgaaa
    21541 ttgatccggc tgatccatca gaaaatgaaa agccagccga gacatattcc cttacaattg
    21601 ctccttcttt gattggtaag atccaactga ctgcttcgac agcggattcc cctgaacagc
    21661 aacctttgga tattacggaa agtggaacgg tagaaggttt attagcggaa acgaaggtaa
    21721 cttacactat tacgccagct gaaggtgaac aaattgcttc agtcgctgtt aatggtgcca
    21781 aagaagtaaa ttatgatgca aagaccttta cagtaggtac gaacaattta tctattactg
    21841 cagaatttaa agctggaccg gatacttcaa cagagaaacc aactgatcct agtgaagaag
    21901 tccctagtac accagatccg gaaacgccaa acaaaccaga tgtgggtaat aacaacaata
    21961 ataatggttc aaataattca aacaataata acaataattc gaacagcggg aattcttcga
    22021 atcaaacaaa cccatctgga tcgaataaca atggaaatac tcagagagaa ccaaaccatc
    22081 caaacaatgg ttcatcgaat ggcggaacga acaatgcttc atctcgtcca aatacagcaa
    22141 atcaaagcgg tatagagaat ccatcttctg cttcttctga ttttgttgta aaatcaccaa
    22201 ttgatgctgt attgcctaca aatacaacga atgtccaaca agcaatcgtt cgagaagcat
    22261 tcaagcatct tggaaaacca tatgtatggg gagcaaaagg tccaaatact tttgactgtt
    22321 ctggtttgac ttattacgta tatatgaaag caaccggtca ttatatcggt ggctggacag
    22381 gcgaacagca atatgcaggt acgcagattc cagtaagtca ggcccaacct ggcgacctcg
    22441 tcttctgggg accttcttcc ggtgttactc accatgtagg tatctatatt gggaacggac
    22501 aatttatcca tgcgcctcaa ccaggagata aagttcgagt aacttctatt tcagatttca
    22561 cgcctgattt tgctgtccga gtaaacttag caggtttacc tgctgcttca ggttcgctga
    22621 caggcggctc atctatttta gatggattag atggctcgtt ccatttcagt caaaaccaaa
    22681 caactgatca gttcttgaaa aagattgcag atgatgcaca ggaaatcggg caaaaagaag
    22741 gaatctacgc atcagtcatg atggctcaag ctatccttga aagtggatct ggaaacagct
    22801 tattatcaag cgaaccaaat cataaccttt ttggtatcaa aggatcatat aaaggaagca
    22861 gcgtgacctt caatacactt gaacaagata gttccggaca aagctaccaa atccgcgcac
    22921 aattccgtaa atatccttct tacaaggaaa gtttggaaga ctatgccgac ttgatcaaaa
    22981 atggactgac tggcaatcct gatttctaca aaccaacttg gaagtcggaa acaaaagact
    23041 acaaagaagc aaccaaatat ttggaaggac gttatgctac agatcgccaa tattcacaaa
    23101 aattgaacgc gatcatcgaa gcgtacgacc tgacaaaata tgatgaaccg aaaaaagaag
    23161 cagcaacaac tgaagaagca aacgctgaaa tcctcaatat gtctaagcga ttcgatgtgc
    23221 caatcaaatg gacaacagat ggcgtgccaa tcacaggatt agaattttca acgataaaac
    23281 gcgtcaacaa cgcttcttcc atcttccgtg tcgcttcaat ctgggatttg tggaacaatt
    23341 ttacggcaag acagatacca gaaacaacta ctcgtacagt taaagctgca actgttcctg
    23401 tactaacctt gttaagttta gacaaatcat tctggcttga tctgccttta aagaaataga
    23461 ataaattaaa aaggggatgt gacactgtca cactcccttt ttaatttatt ctatttcttt
    23521 atatttattt tgtattccgt tacaaactcg cgtacttggt taggttccag agtgacatct
    23581 ccaaaacttt caagtcgctc cgctcctggc agttcttgag tctcaaatgt gataccgcca
    23641 tgatttacta atttattccc gcgcatttcc ggtccatctt ctccaaaatt tgctgtaaaa
    23701 atcacgattc ctgaagcatc tgtttttact tggatcgata ctttttcatc tgggcttgtc
    23761 aattccgcag catttagtaa accagattgt tttttcaaca aaaatggatg atcgatgccg
    23821 cccactatca ttgattggcg aaaatcactg gcaaagacct cacctaattt tttaggacgt
    23881 tggaagtcaa atgctgtgcc acttactgaa actacttctc ctgtaggcgt tgtatcttta
    23941 ttgagcgtag caaataatcg gctattcaac caaagtatat gttcgtcgat tgactgtgct
    24001 ggatcaccgg ttaaattgaa atagacatga tttgtaggat tgaatactgt tttgcggtca
    24061 ctgatccctt ggaccgtcac tcgccatgta tcatcgttcg tcagcgtata ccgtacttct
    24121 acatctaaag ttccaggaaa tccgttttcc atatctgggc tagtctgata gaaaatgacc
    24181 gatgcttcgt tttcaccatt gatgactgta taatgccact gcttggcttc aaagctaggt
    24241 gcaccaccat gtaaacaatg accagtgtcc gggtctgttt ttacttcgaa cgtttcatca
    24301 tctaatttga atgtgccatt cgtaatccgt ccagccatac gtccgattga agcaccgata
    24361 taagggtctt tttctagata ttcttttgct tcatcaaaac ctaagatcaa ctctcgtttt
    24421 tcattatcga ttattgtttg aaaatgaaca acccgtgccc caagatcact gacagacaaa
    24481 gatgtcccat tttgattcgt gatcgtaatc aagtgatagt tttcgccaaa attttgttca
    24541 ttgatttcca tgttccttgc accctctttt ctttcatttg cactgattcc agtatacatg
    24601 gaaaccgttt tcttaacagc ttattccgtg agtaacgaga aaaaatttac tatattctaa
    24661 taaaagaccg tgaaaaaaag gttgtgacaa aaatattgtc acaacctctt tttccgaaca
    24721 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagga
    24781 gctatttttg cttggctgtt cttattactt gatgcaggac agctttttca caatctcttt
    24841 ttcaatacga ttccagtata taaccatgtc gtcagtacat agtagatgaa atacaatacg
    24901 aagaatatcg taaacggaac ccaaatatct ttgtatggat tttgcatgaa tagaccaaac
    24961 atttgtaatc cgaataagac atggactaca cctaagattc ctggtgctaa gaataacacg
    25021 cctacttctc tacggatcga ttgtttaagc aacccgcgtc gtgttcctat cttttcaagc
    25081 atttcataac gagcaatgtc actcttgctt cctgacaaga tcttgaacat caaacagcta
    25141 gcaagcatgg tcaagaaagc taaacctagg aagaatccca taaattcaaa accagagaat
    25201 agaccattat acacttcata catttcatat ttctggttgc tgtactctac tttttgcaaa
    25261 gatggattgt tcattttatt ttgagcaaca agtgtttcga ttggtttcag gtttgcttgg
    25321 aagtctttta cctgaactag ctgtaaagtg gattgtggca agttcagttg atcaaattct
    25381 gattgactga ctaccttgat ttctttttct tgctgttccg gcaattcata gcttcgaagt
    25441 tcctcttgta cagtcagttc attcattttt ttagcaggaa ctttttcttt cgtgatcttc
    25501 ggcggtgttc catcttcatg ttttatcatg atcaatggtt gagccgcaaa ttcacttgca
    25561 ttatagtaaa tcgttcccgc atcttctttt tgagtataag aagcagaaag tgttggtgaa
    25621 agttctttga ttttttgctg atcttctgcc cgaggattat tcatcactag atcatatcca
    25681 gaattgtttt ctgtcaagtc tgcaatttca tttttaaaac ctaatcctac cgtcaaagcg
    25741 cccagtgcca aggcaaacag catagcgacc attgagagca tttgtgtata ttcacgaata
    25801 cgaaagttta actgtgacaa tgtgaaattg ttcaattttt tcaatgaaat ctgttctgat
    25861 ttctttagta atgataaaaa gaaaatgatc acagaatgga aaatgaaata agttcctaat
    25921 acgatcgtaa ctaaggctac ccctatacca aagacttgat aggtccccac gtgtttcatc
    25981 atatagtaac cgcagcctag aaaagcaatt cccagtacta cttcaaggaa aaacaagaat
    26041 ttatgctgct tgattcttgt tggtgtctga tccgcacgaa tcaatgctaa aatcggtttt
    26101 ttgacaatcg aaaaagcatt gacaacagca gctaataaaa acaaaacagc aaaaaacaaa
    26161 acagtgatca acaacccgtg aatactaaaa ggtgtaaaat gcgaaatatt gagatctaac
    26221 tgatccacta ataatcggtg gacgatactt gtcaatccta caccgacagc tgaacccact
    26281 aaagtagctg cgatccctac taagaatgtt tcagaaaaaa tcatctgtgc gatttttctg
    26341 ccttttgctc ccagcatcat aaacattgca tagtcttttt gccgcatcgt catcaaaaag
    26401 gaatttgcat ataaaatata aacgaatgtg atgatcccta gtaaaacaga accgaatcgg
    26461 aaaatgataa ctgtagccgc aacgatcgta ttacttttca aaaaagcttc gttgcttgcc
    26521 attgattcaa acatgtaaaa aatagcagat gccataacta atcctgagaa taacaccaag
    26581 tagtctctta aacggctctt gatcccagta agtgataatt tccaaagcat agtcaatctc
    26641 cccctactgt tccaagtttc caagaataga tagtatttct cgatagaagg aatcacggct
    26701 ttgatctcca cgtttcactt cttgatggat accgccatcc ttgatgaata aaatacgttg
    26761 gcaataactt gcagaaaacg gatcatgcgt cacaagaaga atcgatacat gatcttttgt
    26821 attcaattcg tccatcgtat ctaaaagatc ccgggcactt ttggaatcca gcgcacctgt
    26881 aggctcatcc cctaatagaa tcgttggttg tgtgatcaat gcacgtgcgg cggcgacccg
    26941 ttgtttttgt ccaccggaaa tctcagaagg atagctttcc aaaatatgag agatggataa
    27001 acgttctgca attttttgga cacgttgctt gatttctttt ggtctgacgc cttgtaaaga
    27061 aagaggaaca gcgatatttt cttgtgctgt caagctttct aataaattga aatcttggaa
    27121 aataaagcca atctcattcg cacgaaaatc tgctagttga tttcctttta atgtggtcac
    27181 atctttttga ttgattcgga tatgcccatc tgttggttta tctaatgtgg ataaaatatt
    27241 caaaagtgtc gacttaccag aaccagaagc gcccatgatc ccaataaatt cgcctttttc
    27301 tacatcgaag ctgatgccgc ttaatgcttg cgtctttttc tcattgtttt ttccgtatac
    27361 ttttgttaca ttctgtactt cgactatttt gttcatttct ttccctaccc tttaattagt
    27421 ttttccagtg ctgttttcgg cacgtctttt ttttcgactt cttcatacga agtgacacct
    27481 ttatttttgt tccaggtgat ttttaaatat gctcctttac gaagcggtcg agtcaattgt
    27541 cccttgaagt ccagttcttt ttccttaccc tctttcgtat agccaggcag gatatatcta
    27601 tagttgactg tttcttgtcc agagtctgat tttgaaactg tttttgtacc atctgtcgta
    27661 atttgtacat agtattcttc tccacccata actacgtaat ctgcgatttt cacccctgca
    27721 agtaccacaa cgatgaaaac aagtagaccg atcagaagtt ttttcacttt ttccccttct
    27781 ctcttacttt atatactgat tatagaaaaa agcgaacaat tctgccttcg aatcaactat
    27841 cattcttctt tcaattttgt cattttcttt aaaacaacta aaaaaagagt tcaagacaat
    27901 aatccgtcct aaactccttc ttcctatctt acaaaaagta tgtcacttac ggtctttcat
    27961 gtcgcctgta ttgataacag gttgcgttcc tttgtctgta atgattgaga caagtaaatt
    28021 attgatcaat tggactttcc gatcatcggt gaaattgatt tcttgtccat cttcgatttg
    28081 ctctaaggcc atttgagtga ttgtgacagc tccttcaaca atggtttgtc gagcagacaa
    28141 aatggctttt gcttgttgac gttggagcat ggcactagcg atttctgttg cataggctaa
    28201 atggttcaag cgtgtttcga ttacttcgac acctgctact gctaaacgct cttgcagctc
    28261 tttttgcaac tcgtcagaca ctgccgtcgt gtttcctcga agcgtcaaat cattctcatt
    28321 gaatgtatca tacgggtatt ggctagcgat atgccgaatg gctgtctcac tttggatttc
    28381 tacaaaatct tgataatacg ctacatcgaa taatgccttt gctgtatcaa cgactttaaa
    28441 gactactact gctgagattt cgatggggtt tccatccaaa tcattgactt ttaaaacaga
    28501 actattgaaa ttccgaactt tcaacgaaac agtcattttt tgtgccaaag gtatcgtaag
    28561 gaaaaagcca ttttctcgaa tcgtacctaa atattgtcca aaaaacaaaa ttacttttgc
    28621 ttgattagga ctgactacag ttgccgaact taacaacaat aacgtgatca accaaagaaa
    28681 aatacttaaa aaaagtgccc aaactttcgc ttgccacata cctaaataga agaaaaacaa
    28741 tccaactaaa gctaatacga ccaatccgac aatccctgca taaccattga tataaaatgc
    28801 tcttttttct ctcatgctac ttcctgcgct cctttatcct tcaataaata tctaatgtta
    28861 cttttttaat ttttttttcg aatacgcgcc attacacctt ctagttctaa ctctttgtct
    28921 tcagtgatgt accaaccgct tgcctgtgtc cctccggctg cctgcggtaa ataatccaat
    28981 tttgttcgaa cccagtgctt ttcttctgta aaatagtctt ctaccaagac ctctagatcc
    29041 atccctgctg aaagaggcaa atcttcccga acagatttaa agataaatcg ttccttcttc
    29101 ccatcatact tcaatattcc tgtatcgata atcggtaaag aataataatc cagctctttc
    29161 ttcaccgtca ataaatgttt gaaaacttca tggtacactt gctctaattg gcgaccttct
    29221 gaattgttcg ggtaattaac catcactttt tctttgtctg gatcatagaa ggtttggctt
    29281 agttgagtga tataagtatg taatctttcc atttctgttt ttaacttttc tctttgacct
    29341 tcttctacct tcatatactc tttcaccctg ttttcttttt agatattttc tttcttattt
    29401 tactatagtt atgtgcgtta agcgatgaaa aacagcaaag attggaagga tctttgctgt
    29461 tttttcttaa aatatccagt tgagtacagg atcaatctgt ttcattgtcc tcttgatccg
    29521 tcgtaatatt gatcgatgtg tctttagcag tgatctttag ctttttctgt ccttgttcta
    29581 acaaagtatt tgttattggc tggtagtctt tttcaatata agattcacca gacgtttgga
    29641 tagtatagcc actgttttcc atctcatgca aagataaatt gccaccgttt aggatgactt
    29701 ttccttcttt gctgatcaaa ctgtcttcta gatggaagtt gctgttttca cctgtgacga
    29761 tcaagttatc cacattggcc tcatctaatc gaagtgagcc taattcatct ttcgcagtta
    29821 ttgttcctcc tgtaaattca ttgaacgata cccagccatc atgtgtctta gtattcactt
    29881 ttttgattga tacttcgttg atatcaattg cactattatt cccttcgatt gtcaattcag
    29941 ataattcatg atctgcaggg acaacaatag tgatttgtgc acttgtgtct tctaaactaa
    30001 tgattgcacc tgttgatttt ttgactgcat tcacagtcaa cacgccattt ttctccttta
    30061 tttctggctt ttctgtctta tcataagtcg tttttatctc aaaatccaat ccgcgtatga
    30121 ccgaaactgt ttgattttct gcatggatga cgattttgtt tgcatccttg attttttttg
    30181 tctcagtaac catatcgatc aaccgaggac cattatccca agtcagactc ttttgagcgc
    30241 ctaatgtata agttgcacct gctaggaggc caccagtaag catcagacca ataccggtaa
    30301 tcaaaaaatg tttcaatctc aatttatgtg tcctccttca aacgcccgct gaatgcagat
    30361 ctctttttgt aataaaacgt cgtcccatcc atttaacaaa gttcatcatg acataaaaaa
    30421 gcccttttgt tcctttcagc aacagtggcg aaataaatac tgcgcttcct gtggcgaaca
    30481 gaccgacacc gataaagaat aatccactca gaatcgattg tccaaacaca ctgattcctg
    30541 agatgatcat gaataggcca acacctaaga tactgataca taaaacatag atactaaaaa
    30601 tgagcgctag taaagcaacc aatagtcccc caataaccaa tataaacccc agtgctagtg
    30661 gaatgaggac aggagaagca aataaaccga taatgattaa ccaaatcagg cgaatttgtc
    30721 tttttggatt tttcactaac tcgttttctg cttgttcttc ttctgaaaag taactgattt
    30781 ttaattttaa ggcaaactgc tttggtgtac catattcatc aatgatctga tctaatgttt
    30841 ttccagaatc taaaaaatat tcctcataat actggatcaa atcaaaacga tcgtcttcag
    30901 gaatttcttg aagatgatcc acaacttcat tcaaatattc attcatttgt gctctcctcc
    30961 aataaccggt ctaaagaatc tcgaaaaaca atccattcat caataatttc ttctaaatga
    31021 cgacttccca ttggtgtcaa cgagtaatat cgacggtttc tgccttcaaa aggttcatca
    31081 taggtctcaa gatatccttc ttttttcaat cttctcaaaa ccggatatag tgtagattca
    31141 ctgacagaga gactttcttg tacgattttt gttaaggcat acccatacat atcttccttg
    31201 cttaatgctg ctagcacagt accatcaaga agaacagttg gaatctggat gtgcattacg
    31261 tcaccttcta ttctcaagtg taatatttta tttcaatcta tattatacaa catataatat
    31321 agtttcaaaa tcagtttgtc aaatacaaaa aaactgacta aaagatttga tcctttttag
    31381 tcagcaaaaa atggcttctc tttgattttt attataacag tggcataatc caatttgtcc
    31441 acatccagct cagtggcatg ataacgatca ttgcaggtat catgtcagct actgggaaca
    31501 ttttcacgtt gatcattcta aaaccggtag cgaccatcaa gaatccgccg caagctttaa
    31561 aatctaagat catgtctggt gtagtcaatg gataaataaa ttttgccaat aagaacaaaa
    31621 tataaaaaat aatgaattgg ggaattgcta tcacagaaac gacataccct aaattacacg
    31681 caaaaatagc tgctgtgaaa aaatcaagga cagatttaga aatcaaaatc gtactgtctc
    31741 ctgtcatccc agaatctaaa cttccataga tccccgtacc acttgcacaa aatagaacaa
    31801 tgaccgtcac taatgtgctg ataaattcat cgtgggtcat accaagcttt tcactaggga
    31861 agattttaga aataggtact tgcatcagcg cagccccctt atttatccca ttacctaaat
    31921 gaacaatcaa accaatcgct gttcctatca ctaaagcaaa aataacagcc ggcatatttt
    31981 tcatcggcgc aatggaatag attcccatcc ccatcgaaca cacaccaaaa accatcgtca
    32041 gctgtgcttt aaaatgttca cttaatttat ttcctactaa tccgccaatt agtccgccaa
    32101 acaaaacgga acctacattg atcaaaactc cagttggcat caaaaaactc ctctcttttt
    32161 ctacgaagcg aaaaaaatca gaaaaatttc tgtaattatt gctttcgctg caagaaagat
    32221 tgtaacagac agagaagatt acaccaattc attttttgtg ttaaagtatt ctttattgga
    32281 ataagtagga ggaatatgat ggatatccat aaattcgaag tttttcttga tttagccgaa
    32341 acaatgaatt tcaccaaaac tgctgatcga caattcacca cccaaggaaa tatttctaaa
    32401 cagattcttt cgttagaaac agaattagac gtcaaattat tcgaacgcgc ccatagaaaa
    32461 atcgaattga ccgaagctgg aagcttattg ctcccatatg taaaaaatgt cgtagaacaa
    32521 taccatgcta tgcaagaagt gcttgaaacc tatacgaaag acaacaatct ttcattaaat
    32581 atcttaacga taccgacaat gatcaattat aaaggttttt ccaaaatcac cgagtttttg
    32641 aaacgccatc ctgaatttac agtccagttg aaagaagtgg aaagtgtcga attaacttac
    32701 aatgacgcca atgaacatag tgacacgatc cactttgcta gaagtttcca ggccccacca
    32761 agttcgattg agtggttgcc tactgaaaca gacgattttg tagcagttct acctaaaaat
    32821 catcctttag ctagtaaaaa gaaattggat ctaagcgaac tgaaaaagga gaactttttg
    32881 ctccttggcc ccactacgaa cttgtatcaa cctgtactag atctgtgtcg cgaagctgga
    32941 tttgaaccaa aaatctctta taaaggagca agaatcgact tgatcatcaa tatgattgca
    33001 aatgacatgg ggattgctat tgttatggaa aaaacagtca agaatctatt aaaagaaaat
    33061 actgtgattc tccctatttc tccaacaaca gagagttatc ttgcatttac tcgtaaagtc
    33121 ggtgagcatt ctttggcttc agatacattt tgggagtatt taaatcaaga agaaaactaa
    33181 gccctacatt ttctgtccat ctatccaccc aaaaaaagag agcgacgcat gactttcgct
    33241 ctctttcttg catcatgtcc ttgtttcaat gacacctcta acatttctcg tagttcaata
    33301 agaaagcagc gatttttatg ccaaaagaac cgtaagaaca tttccctaat cacaaaaaaa
    33361 cgactaaaaa cccctaaaaa ccttcaatag aatttttagg ggttttattc tttttcctat
    33421 tgtttactta accgtatcat tgctgtcgtt cctactcctt cgatactgct ataatcaata
    33481 cgtccatcat gcagttctag aattttttta accagtgaaa gtcctagccc attcccagaa
    33541 atttgacgac ttgtatctcc ttgataaaat ttttcaaaca ttcgatctct ggtctcttca
    33601 ttcattccta cacctgaatc ggtcacttcc agtacaatct ctgtggctgt ctcaaatagt
    33661 ttgatatgaa tctgtccatt gatttgattg tactttatgg cgttatccat gatattcagc
    33721 cacacttggt acaaaaactc ttcattgcct gtgtaattta ctgcagcaag ttcgatatcc
    33781 aattctagct gttctttctc ccatttaggc tgaaaaaaaa cgattacttc tctgatttgt
    33841 tcatctaatc gatactcttt cttttccagt tggatacgct ggttttccag tttgttcaat
    33901 tttaagatgt tctctgttaa ttgtgaaagc tgcgtgatac tttcgataat ccgatataaa
    33961 aatatttggc gttcttcgtc agagagattt ggggcttgta acaattgaac atatccttgg
    34021 attgttgcta gtggtgtttt gaattcatgg gatacactgg acacaaagtc attcctcagt
    34081 gtctcgatac tattgagttc ttggaccata acgttgaaat ctttatacag ttgctgtact
    34141 tctgctactt tctgctgttc atccattcga atcgaaaaat ctcccgttgc taccagactc
    34201 atatttgtcc ctagcgcact gattggatgc aggatacgcc gcccaacaaa aatcgatatt
    34261 cctgtaccca cgaccatact gaaccccgca aagacgaata acgggtataa cgttggtttt
    34321 ccagccgatt cagcaggcga gatagaatgt cgagacaata agaaaataaa accgagaaaa
    34381 cacagcagtg tcactaagat cgaaatgaag acaatactag caaaatagat ccataattgt
    34441 gaaaatagct tttttttcat ctttaaataa ccgccttata gcctagtccc cgtactgtga
    34501 tgatttgaaa atcttccgtc ttttcaaaac gggtccttaa acgtttgata tgtacatcga
    34561 tcgttcgttc ttctgtattc gtatctaatc cccaaatatc atccatcaac tgttggcgag
    34621 tgaagatctt gttgggataa gacagtaatt tatacagaag cagaaattct ttttgaggca
    34681 gaatcaacga atgctgatct tgtaacactg tataagcatc tagctctaat tttgtttgtc
    34741 cgattatcaa gctactgctg taattgatct ttgaacgtcg aagcaatgct tccacacgta
    34801 ggatcatctc attgacatcg atgggttttg tcatataatc atctgctcct aatttgaacc
    34861 cgcgtttctt atcctcaaaa tcggcctttg ccgtaatcat tagaacaggt gtgtcaaatc
    34921 tggtgctacg taatgagtct agcaagtcat agccatccat tttcggcatc atgatatctg
    34981 taatgattaa ttctatacgt gtcgcttcta gttttttcag cgcatcctct ccattcaatg
    35041 cggtgatgac tgaaaagccc actcgttcca acacagaacg gtaaagcaac tgtaattgcg
    35101 gatcatcttc tactactaaa atacgattca ttgatctccc tctgtttttt gaaaaatttc
    35161 tagatttttt cttgtagttc atcttcagtt aacatttatt tcgtatacta cgatcattgt
    35221 tctgatatac ttattagtat accgaatatt tcaatacggg agaagggtga ttatgcttca
    35281 attaaaagat ataaaaaaat attataaagt cggcgaaacc accaccaaag cattggatgg
    35341 tgtgtctgtc gctttcagac aaaaagaatt cgtagcgatt ttaggtccta gtggttctgg
    35401 taaaacgact atgctgaatg tgattggtgg actagataat tatgattccg gtgatatggt
    35461 aatcaacgga aagtctacta aagactttaa ggacagtgac tgggatgcct accgaaacaa
    35521 ctctatcggt ttcatttttc aaagttacaa cttgattggt catctaggca ttattgaaaa
    35581 cgttgaactt gggatgacat taagtggtgt ttctaaagac gaaaaacgaa aacgggcaga
    35641 agatgcattg caccgagtcg gcttgacaaa tcatatgcat aaaaagccaa atcagttatc
    35701 tggtggacaa atgcagcgag tggccatcgc tcgagcacta gccaatgacc cagatatcct
    35761 tttgtgcgat gaaccaaccg gagcgttaga tacagaaaca agtatccaga tcatggaatt
    35821 gatccaagag ttatcgaaag aaaaattagt gatcatggtc acacataatc ctgaactggc
    35881 ccatcaatac gcagatcgaa tcattgaatt ttctgatggt aagatcttaa cagattccca
    35941 tcctcacatc gaacgaccaa aagatgatca atttaactta cgcagaacaa agatgagttt
    36001 ttggacagcg ttaaagcttt ccttcaacaa tatccgaaca aaaaaaggac gtactttctt
    36061 gacctccttt gcttccagta ttgggatcat cggaatcgcg atcgttctgt ccctttcttc
    36121 tggattccaa aaacaaatcg ataacacgca agctgaaacg atggcgaaat ttccgattac
    36181 tatttctaag gtaacgacta accagactcg tgacgatgcc ggattgggtg caagcaaagc
    36241 ggattatcca gacagcaaaa cgattactgc aaaagtcagc gaagaagata aagcacagca
    36301 cacgaataag attgatcaaa catacgtcga ttacgtaaca gacatcgatc ctaacttaag
    36361 taataatatc ggatttaccc gtactacagg aatcaatctt ttacgtgatg taaatggcaa
    36421 agtacagcct gtcagcttct cgaatcaaaa tcctgatacg gaatctcttt ctttatctag
    36481 tacgatgtca gcaatgacag gtgtgggagt gtcaagtttc cctacacagc ttgacactag
    36541 caaagaaaac tttttaaaag acaattattc tttacttgcc ggaagttatc cagcctcagc
    36601 aacagatgtc gtgctgatcg tagacggcaa taataatact aatatcaacg cattgaagaa
    36661 tcttggattt gatgtgaaag aagacgaaaa acttgatttt gatgatatcg ttggtacaac
    36721 attcaaatta gtaaacaaca atacttacta tacaaaactt ccaactggga attttatccc
    36781 taatacagat tatgatgcga tgtatcagaa tgcttctgat gaattgaaga tctcggggat
    36841 cctacgcgtg aaatcttctt ctacaatgaa tctcttatct ccaggaatcg cttatagcga
    36901 tcaattaacg actcaaatcg tcaacgaaaa caaagaatca gaaatcgtca aagctcaaag
    36961 agacagtggt gtaaatgtct taactactga aaaagttgac gaaaatgcca aacaaacatt
    37021 gcttagttat ttaggtggag acagcttgcc ttcaagtatc atgatttatc cgaacaattt
    37081 tgaagacaaa gaaaagatct tggattatct agatgattac aataaaggca aatcagatga
    37141 agataaaatc atttatactg atttagctgg aacgatgact gaactgactg gcggattgat
    37201 ggatgctatc acttatgtcc ttattgcttt tgcagggatc tctttagtaa caagtatgat
    37261 catgatcagt atcattactt atacatctgt gattgaacgg acaaaagaaa tcggtgtact
    37321 aaaagcttta ggtgcacgta aaaaagacat cactcgcgta tttgacgcag aaacgtgtat
    37381 tttaggtatt tcttctggga tattaggtgt gtttattgca tggctagcta cgttccctat
    37441 caatagcatc ttatacaaca tgacggatct aaaaaatgtt gctcaactaa atcctgtcca
    37501 tgcgattatt ctagtgatcg tctctactat attgacgatg cttggcggtc atcttcctgc
    37561 acgaatggca gcgaaaaaag acgccgctat cgctttgcgt gcagaataag gttgtgacaa
    37621 ccacatttag atttgagtca taagagcgct gaaatagtag aaaacagcaa gaaacgagga
    37681 agtaatatgt tccttgtttc gcgctgtttt ttttaattat tttttataat tctggtatac
    37741 ttttctttcc tttgtaccac aactcttctg gcgtatacct gtcttttcca tttatgaata
    37801 ctgttccatc tgcttcaatc gttacttcag tcccaatcca catattacga gaaagtgcga
    37861 gaagattttt tctttttcct cttccttttt ttccaaattg caaggcatcg gttatctctt
    37921 ctgtccaatg agatttcacg tattttactt ctccgttttc aaatgtaact ttcaaaatat
    37981 tcgctatatt ttccactttt actgctttag gaaatgactc ctccattttt ttcactttcc
    38041 ttcctccaac actaagtatg cgctttcttt tagtgataag tatatcatag tttattctat
    38101 tatcaaaaaa acgcatccat tccattgtag aatactttac cgaacaagtt gaattgtttt
    38161 cctctttttc accttttact atagataggt aaagacaact aaggaggaat ctctcatgac
    38221 agatcaaccg tatgatttaa gaaaagtcat cgaagaacta aaaacacttc caggtcaata
    38281 tcacgaaaca gatattgaaa tcgatcctga agcagaatta tctggtgttt accgttatat
    38341 cggtgctggc ggtacagtaa aacgtccgac tcaagaagga ccagctatga ctttcaacaa
    38401 tatcaaaggt ttccccggca cacgtgtgaa catcggaaca atggctagtc gtaaacgtgt
    38461 aggtgaaatt ttgcaccatg attatcgaac acttggtcgt ttcttgaaag acgctgtaga
    38521 aaatccaatc aaacctgtga aagtagacaa agcagacgct ccagcacaag aagtcgttca
    38581 tctagctacc gatgcagact tcgatattcg caacattttg cctgcaccta caaatactcc
    38641 tgaagatgct ggtccataca tcacaatggg tgtagtctta ggatcagatc cagaaaaaac
    38701 aatgagcgat gtaacgatcc atcgtatggt tctagaagat aaagatacga tcggtatgta
    38761 catcatgcct ggcggacgcc acattggtca cttccaaaaa caattcgaag ccttgaataa
    38821 accaatgcct atcactatca atattggttt agatccagca attgccattg gcacgacttt
    38881 cgaaccacct accactccac ttggctacaa tgaactatgg tgtgcaggag ctttgcgtaa
    38941 cgaacctatt caaatcgttg atggtatagc agtcgatgaa gtgggtattg ctcgtgctga
    39001 attcatcatt gaagcagaaa tcatgccaaa tgaaacaatg caagaagata tcaacacgaa
    39061 ctcaggaaaa gcaatgccag aatttcctgg ctatactggg gatgctaatc cagctgtaca
    39121 agtagtgaaa gtcaaagcag ttactcaccg aaaagacaat ccaatcatgc aaacaacgat
    39181 tggtcccagt gaagaacacg tatctatggc cggtattcct acagaagcaa gtattcttgc
    39241 cttggtcgat aaagctattc caggaaaagt agtaaatgtc tacaatcctc ctgcaggtgg
    39301 tggtaaattg atgaccatca tgcaaatccg taaagaaaac gaagcggatg aaggtattca
    39361 gcgtcaagca gctattcttg ctttatcagc cttcaaagaa ttgaagacag tcattttagt
    39421 cgatgaagat gttgatattt tcgacatgaa cgatgtagtt tggactttca acactcgttt
    39481 ccaaggagat aaagatatca tcgtgatccc aggaatgagg aaccatccat tggacccatc
    39541 agaattacca gaatataatc ctggctctat tcgtttccgt gggataagtg caaaaactat
    39601 ctttgacggt acagttcctt tcgatatgaa agaacatttt gtacgtgcaa aattccaaga
    39661 agtagaagat tgggaaaaat atttgaaata atgaatgatc tgcaactgtt ttgacatcat
    39721 aaaaaggagt gcggaaaatg gaacaattgg cacaacggaa gaaaaaaatc gtgattggtg
    39781 taagtggcgc ttcaggcacc atttatgcca tcaacctagt gaagaaacta aaagaatatc
    39841 cgatgataga aacacacgga atcatcagtc catgggcaaa acagaactta aagctggaat
    39901 ccgacttatc actaactgaa ttcgaaagcc tgtttgatta ccattattca aacaaagatt
    39961 taggtgctgc tgtcgccagc ggatcatttt tgaccgatgg aatgatcatc gtaccggcaa
    40021 gtatgaaaac agtcgcaggt atctctgttg gtttaggcga caacctgatt gcacgtgctg
    40081 ctgatgtaac aatgaaggag caacgaaagt tagtcattgt tcctcgtgaa acgccattaa
    40141 atgccattca cttggaaaac ttgactaaat tagcacgact aggtgtgcaa atcataccac
    40201 caattcctgc tttttataat cagcctcaaa cgatccaaga tcttgttaat caccatacaa
    40261 tgaaactttt ggatcaattt gaaattgtaa atgagcattc aaaacgttgg cagggtgatt
    40321 aatatggaaa aagaaacgat agttgaagta accaaactag gtttcacgat ccaagctgct
    40381 gttaaagtaa tcggtaaaga cttattgatc actttaacag gcggagatac accacatatc
    40441 ggaacagtca ctacattttc tcgtgataca gagatgcaaa ctgtacgttt ccctagccac
    40501 gatggtcggt tccataaaga cgatgtctta gccgaaaaaa tagctgtgat catcaaaact
    40561 gccgttccgg gaagttgtac gatcacttcc ggtatccatg ttgaccatat ctccaatgca
    40621 caaatcaacg cctcttttcc gatgtcagaa gagttaggtt acaagatttt agattggttg
    40681 gaaaaaatag atttccatat caaagaacca gtttattata aagatggtga aaaaccatta
    40741 tgaaagaaga ggttgtgaaa tcagcgttat gacctgagta tgagagaaaa aaatggaaaa
    40801 atagcttctc atattttgcc gtttttgagc taataccgag ggacagctga ttgaacgccg
    40861 tttatcaaga ggttgtgata aagccgcttc gtttcgagta gtaagaaaaa ttttgtaaac
    40921 atgggtttcc acatttttgc ataaatttga cttactaccg agaaactggc ttttgaacac
    40981 cgtttatcaa gaggttgtga cacgtctttt gtcacaacct cttttatttt tgcttcactg
    41041 taactgctac aatatcaccg atttcttttc cgatgttttt acgaatatct tttcgtagtc
    41101 caaggatgta gcagactgaa ccatctggat ttttcacgcc catattcacg atactgcctt
    41161 catacggcac attgtcaaaa gttgcttgga ctttaactct tccagcaccg aattcttttc
    41221 ttatatcata cggaaaaata atatatgccc cgcccttgcc gacttcggaa gcatagattt
    41281 ttgattcaaa agaataaatt tccctcattt tatttctcct attttttaca tctagtatag
    41341 catagcaaaa aaaagaagga actgtgacaa aagtcttgtc gcagttcctt ttttcgaata
    41401 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tagtcaacaa acatgagaag
    41461 ctgttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctcttcc
    41521 ttaaacggtg ttcaaaagct tgcatctacg gcactaagcc caatcagatg aaaaatatgg
    41581 agtgctattt ttatctgatt gttcttactg cttgatgcag agcagctttt tcacaacctc
    41641 ctacatggtg ttcaaaatat tattcgcctt ttgcttcttt cactaatttt gcgaatgctt
    41701 gttgttggaa ttcagcagct aaggctttcg tacgttgttc ttgctctgct ttgctcattt
    41761 ttggattttc agtcattgct tttaccatac cgtcttgaac ataagctttt gcttcacttt
    41821 ccatcgtact ttgctgtttc ttcactaatt cagctaactt ggctgcttgg tccgtactta
    41881 attctaccca tgtctctggg attttgaatg tattcactcg gctgtcatat tcgtgattgt
    41941 tatcagcaat attgaaccag aatttataca tgtcgttttt gtattcccaa taagtagttt
    42001 tcgtcacttt ttcagcatcg ccgtctgtct tttctacttt atttgtttcg ttatccgtac
    42061 cagttgcttt tggtttttct tgtttttcat ttgttcggta aagatacact ttttcttttc
    42121 cattccctaa agctttatac aaaagcatat ctgctccttg ggaatcagca ctggaaacaa
    42181 ttttttctgt ttttgtttcc gttacttttt ccattccaaa gtgattggac aaattagcga
    42241 cgatcaaact tagtgaagcg acgaagatta gtccgaaaat caaagataaa aaggtttgcc
    42301 atgttttttt agcgaacacg ttcgttaaag caaaagcaag caagctgaca atcaaaactc
    42361 caataatcat gcattatccc ccgctttcat tagaacaggt tcttttttct tcaataaaaa
    42421 ggcaataata aagccaagaa tcccaaagat gatcgctacg ataaacgcgg catggtaacc
    42481 tgacagtgtg gcattgattg cctgatcttt ataagctaat ggcgtattat ccaatacaga
    42541 tttgcttggg atatttgctt ttgtcacatt cgtcaacaca ctgatcaaaa tagctgtacc
    42601 aatcgagctt gcgatttgac ggaatgtatt atttaccgct gtcccatgac tgatcaagtt
    42661 atttggcaat gcattcatac cagaagtcgt taccggcatc atgaccatgg agatcccaaa
    42721 catacggatc gcataaagga tcacgatata taaaactgga gtcgtttttg tcaagaaagc
    42781 aaatggtgca gttgccgctg tcaaaatgaa catccctgtg atggctaaac gtttcgcgcc
    42841 gtatttatcg aaaatcgcac ccgtcacagg catcatagcc cccatgatca atgcacccgg
    42901 caacaacatc aagccagaat ggaatgctga ttctccacga atattttgaa tatacaatgg
    42961 tacgaccatt tctgccccta ccatcgccat atttgttaca ccagctaaaa cagctgcaat
    43021 cgtgaatgta gatgatttga atacacgaag ttccaagaat ggatgttcca aatgtaattg
    43081 acgccatgag aacaaggcga tgaaaatcac accaacaact aagaaaccaa ttactttcaa
    43141 gcttccccag ccgtcatctc ctacactaga aaatccgtaa agtaaactac cgaatccaat
    43201 cgttgataaa atagcggata aaatatcaat gctaggattt gacaatggaa taacactttt
    43261 cattaggaag aatgctaaaa ccaagacaag aacaacgatt ggcagtacca taccaaacaa
    43321 gtcacgccaa tggtagttat caatgatata accagataat gtagggccta aagcaggagc
    43381 aagtccaata acgatccctg acatccccat tgctgatcct cttttttctg gcgggaaaat
    43441 agatagcatg atattttgca ataatggcat cgatactccc acaccgcaag cttggatcaa
    43501 acgaccgatt agtaatgtac taaaattagg tgctgtaaag caagtaatcg ttccgatcaa
    43561 aaagatcgtc atggcagaaa gatataaatt tcttgaactg aatttattga tcagccatgc
    43621 tgttattggg atcatgatcc cgttgactag caagaaacca gtcgttaacc attgcacatc
    43681 agacgctgaa atatcgaaag ctttcatcaa tgtaggaaaa gctgtcgtca ataaggtctg
    43741 attcaaaacg gtacaaaatg taccaattaa taagacaaca actaaaagtg tccgattata
    43801 tggttttccg taaatatcta cgggttggtt gttactcata tttctccttc tttctgaatt
    43861 ttttctgaaa ctactagaat actttagtcc taaattcgct atttgtcaac ctacttgaca
    43921 cataagttaa tacggatata attaagggaa gcaagaggac aaaaggagtg acaaaaatgg
    43981 ttggggcttc ccttaaacgt ttgttggaaa atgatcaatt ggttgttggc atcagtgaat
    44041 taagtgatat cgtaggtgtg tctccacgtc aattaaggta ttgggaacaa aagggattca
    44101 ttcgttcgat cgaagctgat gcgaattgtc ctagaaaata tcgtttacct acagtcataa
    44161 aagtagaact gatcaaaaaa tatttagatg acggatttac tttgtcaaaa gctgcagaaa
    44221 aggcagaaat ccgccaaaag aaaatgcacc atgtccgtaa agttttttca aaagcaatca
    44281 aagatatcga attgatcgac gaacgctata cgattttaca aatcggtaac ttcaaagaag
    44341 agaatgctaa gatgtacatc attcatgaca acgagacaga cgaattaact tacaagctcc
    44401 ttcctgtcga tgaaaaagct gactacgaac agttgttaaa agaactctaa acgccactaa
    44461 tctcctaagt accaagcttt ttaataagta gatgctacat aaatacttga aaaaaggcct
    44521 gtttgaacat aagaatatcc gaacggtgat ctgcgtttct ttcatataga aaggaattct
    44581 ccatcactgt tcggatattt ttttattttt ttcggttact ttcttctaat acatctgtta
    44641 gccaatttgc atattctgtt tcccgattga tcgcatgcgc taaaatcagg tagtgtccgt
    44701 aattttcatg tttttctttt tcagtaggaa agatcgtttt ttttcgctct aatagatgaa
    44761 gtcgtttttc ttcatgaagg tcacgctgct gctgaataat ttctactaat gccggatcat
    44821 tcacgtcttt gacgaaatac aatttcagaa caaattcgtc tcgattaacc ggtagttcat
    44881 tggtgggtgt atgaatccac tctgccaatt gttccttccc ttcttctgtt aattcataaa
    44941 ctttcttttc caatttttca cctgtgatct ctacttgatg ctgaatgatt ccttgctctt
    45001 ccatttttgc aagttcagga taaatctgac tatgttttgc ttgccaaaat tctcctattt
    45061 catttttgaa cgattgattc aattcatatc ctgtcatctt cttcttactt aataacccta
    45121 acaaaatata tcgaagtgta ttttttcttg ccatcagttt tccctccttg acaaatcttt
    45181 aaatccattc tatcataaat gagtaatcga aaacatgtag tttattacat gttttaattt
    45241 ataaggagat gcttaaacat gaaagaattt aaagaattgg aagattttat tggaactcac
    45301 tttatctata cgtatgacaa tggttgggaa tatgaatggt atgccaaaaa tgaccatact
    45361 gttgattacc gtatccatgg cggaatggtc aaaggccgtt gggtaaaaga ccaagaagct
    45421 gatatcgtta aattgacgga cggcgtattc aaaatcactt ggacagagcc aacagggaca
    45481 gacgtggcat tggactttat gccaaatgaa aagaaacttc acggaatgat ctttttccct
    45541 aaatgggttg aagaacatcc agaaatcact gttactttcc aaaacgaaca tatcgcagaa
    45601 atggaagcag ctcgtgaaaa atatgaaact tatccaaaat tacttgttcc tgaatttgct
    45661 acgatcactt atatgggtga tgcaggtata gacaatgaag atgttattag cgaagaacca
    45721 tacgatggaa tgccagatga tatccgtagc ggaaaatact tcgatgaaaa ttataaacga
    45781 gtaaaataag gttgtgaaag agaggttgtg aaatcagcgt tctgacctga gtattagaga
    45841 aagaaacgga aaaatagttc ctcatgtttt gccgtttttt gagctaatac cgagggtcag
    45901 ctgattgaac accgtttatc aagaggttgt gaaatcagcg ttatgctttg agtaataaga
    45961 acgctagtta gaaaatagct tttcatattt tctaggctag tgggcttatt accgagaagc
    46021 agccgtttga acaccgttta tcaagaggtt gtgaaatcag cgttttgacc tgagtattag
    46081 agaaacaaat ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    46141 cagctgattg aacaccgttt atcaaagatt gtaacaactg catattcaca cagttaaatt
    46201 agtgacttca aaatgaaata atgctcccag caaaaaatta ggctgggagc attatttcgt
    46261 tattctgttc atcaaaaatc agcgttttga ttccatttca tcacaagttc ctcctcattt
    46321 actgcttcat ctttttgctg tgtttgaatt gtaaaacctt gtgaaagata gaaatgaata
    46381 gctgattgat ttttttgata gactgtcaaa gtcagtgttt tattggtttc ttttattttt
    46441 ttcattagtt gcgtaccgat tctttgttgc cggtattccc ttttcacaaa gattcctgcg
    46501 ataaactgat caatcacccc agcaaaacca ataatttctt gatctatttc tgctaagtaa
    46561 atcactgcat ctggtaaagc tttcctaaca gattctactt gattctccca ataagttgat
    46621 ggaatgaatt catgagaatc caagttagca tgcagccaaa tcgatagaac agattccagt
    46681 tctttggcag acaattttgt tgttgtgcgg atcatttttt ctctccttac ttattcattg
    46741 attttaaaat aaaaaccatt tttttgaagc tcttttacaa gttttcgccc ctcaaaaaag
    46801 tatatcttct cttgttttac ttttctaata ccaaaagaaa cagcaacttt aggatcatca
    46861 gctgaaacga ttcgataagt tccaccaggc aattgatcca gcttgacttt cccatgatgg
    46921 tcgctttgta ctgaaaacgg cacaccattt ctagtaagtg tttttttctt atgtttcaaa
    46981 atgaatgaac taccatttac cggtttccct gacgaatcac taataataaa tgaaaaatca
    47041 agcacttttt tacttaatct atactgatag attagtcgtc cgataaacac taacagtatg
    47101 actacagcta ttatcgttcc tgcaagaata aaccattgcc gcatggttct ttcctgattt
    47161 ccttttcctt cttctttttt taccgattcg ttataaggaa tccgatgtcc tgtcactagc
    47221 aaacgatgcg tgttgatcat atacggcgta catgtaagca aggtaactaa gtcttctcct
    47281 tctgaaatcg ttagaacaga cgtgtcatct ggcagtacca cttgtatatt tttaacttga
    47341 taagccaatt tctcccccaa aacagtcaaa ataaacgtgt ctccgttttc taaacgatct
    47401 aattcggtaa ataaccgtcg attaggtaaa ccgctgtgag cagagatcac tgaatgcgtc
    47461 gaatttccac caagcgggaa agaggttcct tgcaagacgc ctgctccatt ttcaaggatc
    47521 ctaccattga gccgatcata aagtgaaata tttaattgga tcttaggtat attgatactt
    47581 ccgatcagcc attcctctaa ttggctggat gttgtacttt catttctgtt cttttcattg
    47641 aaaggatcag taccaggatt gaatccttgg tcagttaatc gttggttttt ctcctccatt
    47701 ttctttcgct gcacttcaat ttcagctgcg gtccgttttt gtacttgttc gatacgtttt
    47761 tgatcaataa aactatttaa agaatccaca taaaatggat ataaagcgat cagtaaccct
    47821 acgacgatac atattaataa aaacaaacga aacaaaagtc gacgagactg tttttgctta
    47881 ctctgcttca catgtgtcct cccttgcaag tttttttgtt ctttctttta ttatatgggt
    47941 tcaggaaaaa aatagaaaat gaaagccgat gaggttgtga aaaagctgtc ctgcatcaag
    48001 taataagtac agcccaaaaa aatagctact catatttttt gttgggttgg gcttattacc
    48061 gcagatgcaa gcttttgaac accgtttaag gaagaggttg tgaaaactgc gttatgcttt
    48121 gagtaataag agcgctagtc agaaaatagc ttctcatatt ttctaggcta gtgagcttat
    48181 taccgagaag cagctgattg aacaccgttt accgagaggt catgaaaaag ctactttgtt
    48241 tcgagtatta agaaaaattc tgtttcaaaa caaggagatg caacaaaact tatgtcgcat
    48301 cctctctcct tagaactttc acgtatgttt ttcttgtaaa attcataaat attttcatga
    48361 attttctaat ttcttgccta cctctcgcat aggatatatg atgaatttat ctaaagtttg
    48421 caaaggagtt cgacaattga aaaaatatgg cccattttta gcaatttttt tcattgcttt
    48481 ggcatttctc atgcctcatt tattcacaca taaactaatt ttgggtgctg atgccccatt
    48541 ccattacaat cggttctatg atgccgcgca acaaattaaa tatggggatt tccattattt
    48601 tccttccctt tatggattcc agcaaagtgg caggatcgtg aatgccgttt acggcccatt
    48661 tttcgcttat ttacaaggat tgatcgtgtt gcttgcaggt agctggtttc attatcagat
    48721 tctttcaaat ttcattcttt atcttatttc aggattttct atgctgattt tactgagata
    48781 tgtcaatgtt cgaaattggg taagtgtacc agtagccgtt ttattcatga gtacgtattc
    48841 tattcaatac tggtctatca accaaggatt cactagctgg gggacttgtt tttatccttt
    48901 atgtatgatc cctattgctg atttcgtcct tcataaaaag ttccctgtta tcaaagtcgc
    48961 tgtctcagtg ggacttatga cacagatcca tttactgtct tcgatcatgt tagctttgat
    49021 gtatatccct ttttatcttt attacttttt taaccagact gaaaaaggaa aagcactgct
    49081 tcaactgatt aagtcggtct tgctttattt tgttctgaca gcaaatattt gggcaggatt
    49141 agctattgtt tacagtggga ataagattct tcctcccttt gttaacagag aaatgagtca
    49201 aaaggcgata gatctggatg gcagttattg gctccattac cctaaggcct ttcctatatt
    49261 gatattatca ggttttttgc tttttatttt ctttcaacaa aagaacacct tattccaaaa
    49321 aatcgtgttg tacacaagtc ttgttttctt ttttctttct actagcttga ttccatggac
    49381 gacattgatc gaaaaaaatg tgccttttat tagtttgatc cagtttccat ttcgattttt
    49441 cgtgccattt acgctattgc ttctacttta ccttgccctt tcactgaacc agtggtctga
    49501 acaaaagtgg ttccgtcttt taagtatatt tggcattctt attttttcag cacagacgat
    49561 ccagaactta taccagcatc tggaaaagtg ggaaaatgaa acatttgtca gtcgacatac
    49621 ttatctattc gatacgccgg aagaagcaag gaaaacattc ttcagcaaag atatgtctac
    49681 cagcttgttc gttttccaaa aaaccacacc tgactatctg ccaatctata aggagacaaa
    49741 agacaataaa tacgaccggt ataacgaaga aatactagaa aacgaaacaa cttttataaa
    49801 aacacatcca ggcggtcttt tgacaatcaa gtggaaaaac actggggaca aaactgttca
    49861 tatccctgtt atcgtctacg atcggactgt gctacagcaa aatggaaaaa cattgactga
    49921 ttatgaagta acagatatcg ggaccccaat cgtgaagcag caaaaaggaa tcaacgagct
    49981 cacgcttcat tatcaaacgc caatctattt ctatttcatt ctttctctta ctttgattgg
    50041 ctggtttacc ttgctatgtt tatttattta tcatagatac aagcttttac gtgcttaggc
    50101 aaaagggata aatacatccg atcaaaaaac tctccaaacg gtataatcgt ttggagagtt
    50161 ttattttaat cagttttttt atgttatatg ttatattttt tattcgttca catctaattc
    50221 gatttccacc ccgtagtgat cactgatgat tggactatcg atcccatcaa aaatccgttg
    50281 atatttttta acctgcattc cttcagggac gaaaataaaa tctatacgca atttttctgt
    50341 attttgttcc caaccatcta ttttcttttc cacagtagct gcttttcctt tttcattcgc
    50401 gacaacatac gaatcttgaa gaagaaggca actttctctt acagtataat aaccttgaga
    50461 gtctactgga ttattgaaat cacctaggaa aaaaagtggt acgcgagttt ccaaaaagta
    50521 gttttccaag tttttccact caaaataaaa gccacttttt ttatctgtcc accaagaaaa
    50581 gtggcagctc gagacaaaaa tgtcctgatc atctatcttt gttttcccaa tcaagatctt
    50641 gcgtgtctga taatcttctt tcttttgaaa ttgggagacg gtaaggacct gactttccaa
    50701 tgtttttttc gtaagcagtg catttccttc ttcatattta tcataaccga tgtggctcat
    50761 ttcccaactc caaaaatact cttttccgtg ttcctttaaa agctgaacta aacggtaggc
    50821 aaaattatct ttacggatag gtacttgatt ctttaccgga cagaacttca ttagcttcgc
    50881 aggttctact tcttcagatt ccagtaattg gttgatttct tgtaaagcaa tcacttcata
    50941 atcttctaac aggatcttat ctgctaattc ttgcaatttt ttctctggtt ctggctcaag
    51001 ccagctatgc gtgttgagtg tcatgaattt cattggtttt ctccttgtct tcatttcgaa
    51061 acaaaagggt tgtgacaaaa tttttgtcac aacctctcca gcaaatagtg ttctcctgct
    51121 aactcctcag taataagctc aatggaccag gagctatttt ttgtttggct gtttttatca
    51181 cttggtgcag ggcagctttt tcacgacctc tccactaaac agtattcaaa ctgctgcttc
    51241 tcggtaataa gcccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta
    51301 tgacttgatg caggacagct ttttcacaac ctctccttac attttaatgg agccgatcac
    51361 tttaccagct gtcactgtac ctaattgctt gatcactact tcttctactt tatcgtttgt
    51421 aaatacgatc atgatcgttg gatctttttc ttcgttttgg atcacatcaa gatccatctc
    51481 agctaatttc gttcctgttc ctacttcttg gccttctttg acagcaatct caaaagctgg
    51541 ctgattcatt tggactgtat cgattcccat atggatcaaa acttcgatac catctttcgt
    51601 ttccagccca attgcatgtt tgctagggaa aatacttgtc acttttccag cgatcggcgc
    51661 aacaacttct cttgatgttg gaataacagc aaagccgtca cccatcattt tttgtgagaa
    51721 taccggatct tttacttcag ataaaggaat cacttctcca gaagctgcgg aaactagttc
    51781 ttgttctata cctaagtatt gcttactacc tgcttgtcca tttcctgtag aaaccatttc
    51841 tggctctgga atctctgctc ctgattgcag cagatcttcg atatctgatt tcaagacatc
    51901 tgcttttggt ccataaactg cttggactcc attgtctttg acgatcaatc ccatcgcccc
    51961 tgctcgtttc caggcttctt cagtccctac tttttctcga tcatttacac ttacgcgtaa
    52021 ccgagtcata caagcatcga catctttgat attttctttt cctcccagca aatgaatgat
    52081 ctggaccact tgctgatctg ctgttcctgt tccgctgtta gatacaacgg tttcttcact
    52141 gtcattgtcg taatttccgt tacgtcccgg tgtagcaaag ttgaattttt tgatcaagaa
    52201 atttgctaag aaatacgtaa caacaccaaa agcgatcaca cacaacacga agttgataag
    52261 atctccacct agacccgctt tgatagctaa cggtgtccga gtcagcaatt cgatattccc
    52321 aaatgaatgg acccgcaatg gcaagatatc tgccatcgca aatgcagccc cttggatcac
    52381 tgaataaatg acatataatg gcacagcagc aaacataaac atgaactcca atggttctgt
    52441 tacacctgtt agaaagacag ccaacgctgc tgaaaaatac attgatttat atcttgcttt
    52501 cttatcagga tcgacattac gatacatcgc aaaagccatt cccatcaaga tacctgaaga
    52561 accaatcatt tgtcctactt tgaaacgagc cggcgtccag ttatgcaaca caaactcata
    52621 ttgcgacata tcccctgcac ctttaagatt cactaaatca gtggcccaag ccaaccataa
    52681 cggatcttgc ccaaatactt gtgtccccgc ctgtgcgcct gataaaatct catacgatcc
    52741 gccaagctgt gtatagttga taggaatcgt cagcatatga tgcaatccaa atggcaatag
    52801 taaacgttct aatgttccaa ataaaaacgg tgctagaatc ggtgcgcttt cttgtgattc
    52861 tgcaatccat aaaccaaaat tattgattcc agcctgtacg tttggccaca ccacagcaag
    52921 aatcaatgcc acgattgttg accataagat aacaacaaac ggaacaaagc gctttccatt
    52981 gaaaaatgac aaagcatccg gcaattttct ataattatag tacttattat aagccattgc
    53041 cccgacaaat ccagcgatga tccctacgaa tactcccata ttcaaagctg gtgcttccaa
    53101 tacacttgta aaaaatcctt taaccatgat cttcgtaccg aacagagtgt gggtaaatgc
    53161 atcttcattc gcaagcatgt cacttgtgac accaaagata gccccagtga tccgattgat
    53221 taaaataaat gagatcccag ctgcaaatgc tccgcctgct ctttcttttg cccaacttcc
    53281 tcctattgct aaagcaaata aaatatgaag attaccaata attgcccaac caatattttc
    53341 aattactccg cccgttgtga caagcggcgt ccaatcagga ttgatcaagg gaattgattt
    53401 ccctatactg atcatcaacc cagctgccgg cataactgct accacaacca ttaaagcctt
    53461 tccaaatttt tgccaaaact caaaactaaa tatctttttc attccgttta gctcctttac
    53521 gcaatcggtt tcgttaaacg tattatagcg tgcgttttct tttttgtaaa tgctttattt
    53581 tttatttttc tgtctatacc attctataaa acctataata cgtgtgttcc cttctataat
    53641 tttctcctaa aaatttattt ttttaagctt ttttctatga aatattttct tttgtctatt
    53701 gacttgtgca atcggttgcg tttattatga acgtataaaa cagctcatta ggaggagaaa
    53761 aatgaaacag atcaaacgac tatttgctat tgatccatgg aaaatcagca cgacacaatt
    53821 agataaaaaa aatcttcgac tgcaagaatc tttaacaagt attggaaacg gttatatggg
    53881 gatgcgcggg aactttgaag aaacttactc aggggaccac catcaaggta cttatttggc
    53941 gggtgtctgg tatcccgata agacacgtgt cggctggtgg aaaaatggtt atccagaata
    54001 ctttggaaaa gtgatcaacg ccttgaactt tatcgcgata gatatttcga tcaacgatca
    54061 gctgatcgac ttggcttctt tggaaccaga agacttttac tgggaattag atatgaaaaa
    54121 tggtgtatta tctcgtacat tcacaatcac tacttctact aataaagtac gcatttcctt
    54181 cgaacgtttt ctgagcattg tcaaaaaaga atcagcttat atctatttaa ctatagaaat
    54241 gcttgaaggt agcggaacag tcaaactcgt atcaaaattg gatggaaatg ttcaaaacga
    54301 agacagcaac tatgatgaac atttttggaa tgagattgat cgtggaacta tagaagaaat
    54361 cggctatttg acgactaaaa ctattccaaa caactttggg attgaacaat ttactgttac
    54421 tgcagcaatg cgccatttcg ttaatggaaa atcagtcatc cctgactatc acgaagatac
    54481 tttagcatta catggttgct tgtctgcaga attatctcaa ggtgagacat ggtcagtgga
    54541 aaaagaaatt atcgttctta ctagtagaga tattccagaa gcgcagcaag cagaaaaagc
    54601 tgtctcagaa ttaacgaacc agacaaacaa ttatatccaa gcaaaagaag agcaaacagc
    54661 ggcttgggca gaacgatgga agcttgccga tgtggtcatc gaaggagacg aagaagcaca
    54721 acaaggtatt cgattcaatt tgttccaact attctctact tactacggcg aagatgaacg
    54781 attgaatatc ggccccaaag gattcacagg agaaaaatac ggcggagcca cttactggga
    54841 tacagaagcg tatgctgtcc ctctttatct tgctttagcc aaacctgaag taactaagaa
    54901 tctgctgaaa tatcggcata atcaactgcc gcaagcaatc cacaacgctc agcaacaagg
    54961 attaaaaggc gcgctttatc ctatggtcac cttcactggt gtcgaatgcc ataacgagtg
    55021 ggaaatcaca ttcgaggaga ttcaccgaaa tggcgcgatt gcctatgcaa tctacaatta
    55081 tacgaattac accggcgata cggaatattt gaaaaatgaa ggactggaag ttttagcaga
    55141 aatcgcacgc ttctgggcag accgtgtcca tttttcaaaa cgagcaaata aatacatgat
    55201 ccacggtgtg accggcccaa acgaatatga gaacaatatc aataacaatt ggtatacaaa
    55261 tacgatcgca gcttgggtct tgcgttacac gcgagaaaat tatttaactt atcaaaaaga
    55321 agctgctatc actatcagcg aagaagaact agccaaatgg aaagatatcg cagagaacat
    55381 gtacttccca gttgacgaag aattaggaat ctttgtccag catgacacat tcttagataa
    55441 agatctcatg cctgtatccg atattcccac aagcgatctt cctctgaatc agcattggtc
    55501 ttgggacaaa atccttagat cttgcttcat caagcaggca gatgtactac aggggattta
    55561 tttcttcaat gatactttta caaaagaaga aaagcaaaaa aactttgatt tctatgaacc
    55621 aatgactgtc catgagtctt ccctttcgcc aagtatccat tcgattttag cagcagaatt
    55681 aggaatggaa agcaaagctg tagaaatgta ccagcgaaca gctcgcttgg atttggataa
    55741 ttacaataac gacacagaag acggcttgca catcacttcc atgactggta gctggttgtc
    55801 gattgtccaa ggatttgctc aaatgaaaac tgcccatgat cagttgagat tcgcaccatt
    55861 tctgccaaaa acttggaaag cttattcttt ccatatcaat tatcgcggac gtttattgtt
    55921 catctcagtc acagatgaac aagttacttt cgagttatta gaaggttctc ctctagaaat
    55981 ttacgtctat gaacagtctt attctttagc ggatcgtcta gaaataacgc taagaaagga
    56041 ggaagcaaat gtttaaaggg gtattgtttg atttagacgg tgtcattacc gatactgctg
    56101 aattccatta tcgtgcttgg cgaaagcttg gtcaggaaat cggcattacg atcgaccaca
    56161 cattcaacga acagctaaaa ggcgtcagcc gagaagactc actctctttg cttttagctt
    56221 atggcggaaa agaacactct ttttcaaagg aagaatttgc agaattagcg aaaagaaaaa
    56281 acgattatta tttagagatg atccagacga tcgaaccaaa agatgtcttc cccggggtcg
    56341 tgcctttact ggattcttta aaagaagaaa aaatcaagat tgctcttgct tctgcaagta
    56401 aaaacgggcc attcctactt gaaaaaatgg gtcttacacc ttactttgat gctattgccg
    56461 acccggcaga agcaacaaac ggaaaacctg cgccagatat cttcctcctt gcagcaaaag
    56521 ctgtcggact aaaagcagaa gaatgtatcg ggatcgaaga cgcgcaagcc ggtatccaag
    56581 ccatcctctc aagtggcgct caaccaattg gcgtgggacg tgctgaagac cttggagagg
    56641 agatcccgct tgtccctgat acgacttttc ttacgatcga ttacttgaaa gaaaagtggc
    56701 atgaccatgg ataatccaga gatctccgtc gtaccagttc cagagactga aataaagaaa
    56761 atcacattga agaacgacta cttagaagtc gttcttctca attacggtgc ccgcctgcac
    56821 caaatcttcg caccagacaa agaaggaaaa tcagaaaatg ttttattgtc ttacgatcgt
    56881 ttcgaagatg ttctaacaga caaaagcttt tttggagcaa ctgtcggacc tgtagccgga
    56941 aggatccggg atgcttcttg gggtgagcat agattggaga aaaactgcgg atcacaccat
    57001 atccacggtg gcacgaaagg ctggtctttc caattttggg aagtagaagt tttcaaaact
    57061 ccatcatcaa taggtgtggt cttttactta aaagatgaat tttctaccta tccaggacca
    57121 atcactgcta cgatcactta ccgtttaaca aaaaacgaat tagagatgac aacctctgct
    57181 agtagccttg ttgaaaccat ctgcaatccg accaaccatg cctacttcaa cctttcagga
    57241 aatggaacaa gagacatcta tgaacatcag ttaacggtct ttttagatgg tgtgcttgaa
    57301 ttagatcaag aaaaactgcc tactggaaat tggacaaaaa aagaaaaact gccaatcgac
    57361 tttagaaaat ccccaacact gcaagagatc cttgcatgtt atccagatgg actagatgat
    57421 gtatttcttt tgcatcatcc taggctttcc aaaacttcct tacaattatt cgagaagcat
    57481 tctggtcgcc aaatgactat agcaacttct aataaaagta tggtattatt ttctacgact
    57541 ggattcgaag ctgatttctc tgtcaatgga aaacagatgc attcaaacta cggattagca
    57601 atcgaaccgc aagaaatccc tgatatcgtt catttcccca aatttggatc gatcaacctc
    57661 catcctggtc aagaacggat cagtcagacg atttatcggt tctctgcaca ataacaagag
    57721 aaaggtgttt ttatcatggc tattaccgta aaagatgtgg cgaaaaaagc cggtgttgcc
    57781 acctcgaccg tctctcgtgt catcaacgat catcctagta tttccgagac aacaaagaag
    57841 aaagtccgaa aaattatgga tgaactaggt tatgttccta acatgactgc ccgcaatcta
    57901 ggaaaacgca tttccagtgc catcggcgta atcttacccc ctcttgattc aaaagagcgg
    57961 atcggtaacc cattttatct tgagatcatg gaagcaatca acgaagaagc gcggaattat
    58021 gatatgacta cagcaatcgc tactgctaaa aattttgaat tgctgttaga gaacgtccag
    58081 cgtatgcatt tacaaaaaca agtagatggt tttattctag tgtattcaga tagtcaagat
    58141 actgtcattg attatttata ccaaaaccac atccctttca ctttgatcgg acagccttat
    58201 catcatgaga acgaaatcgt ttatgtagat aatgataacc agcttctagg taaacaagca
    58261 acagaatttt tgatccagaa tggacataag aatatcctgt ttgtgacaaa cacgactcat
    58321 gaaagtttgt atttcgaacg atatttcggt tatcaaaaag caatgatgat ggctggtctt
    58381 cctgcgttat ccagtgtagt actagagcat ccggaggatt atctgtcctt tgagaaaacg
    58441 ttgaaagaaa acgaagccac agcattggtg gtcattgacg atatcttcgc ccttcgcacg
    58501 atgcagttgg cgcaaatgta tggctatgtc attccagaga cactctccgt cattagcttc
    58561 aacaactcga tcttctcaac attaacacac ccttacctta caagtatcga tatcgatatc
    58621 tccgaattag gacgtgtcgc tatgcaaaag ctgcgcgaat tgatccatga agaattgagc
    58681 agtggcgtac gtttggtcat ccctcatcgc ttgatcaaaa gagagacagt cgttccttta
    58741 gaaaacccat actaaatagc tgactcacta aatacgctga ccatttatcc tatggtcagc
    58801 tttttatgta aaagaagata ctacttctaa cacattgttt atctgtaaaa aagagcgtgg
    58861 gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaga
    58921 agcaactcct tcggaaataa gctgaaattc acaaaaattt aaaaagcaat tttcttgagt
    58981 ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tcttttttcc ttccttcaat
    59041 tttttacacg tcgacgattc aataaaaaac cgcctaatat tgtaaagaca ggtgcaagaa
    59101 tcgagaaaaa ggcgaatggc aagtaatgca acgcgctaac tttcatcgtt cccataatga
    59161 atgtgccgct gactccccac ggtacgagag aattgacggt cgcacctgaa tccgctagtg
    59221 ttcgtgtcaa ataggttttt gataagccaa cgtcatcaaa agaacgcttg aatgtttctc
    59281 ctggaagaat gatggacaga tactgttctc caacgatcag attgattccg atacagctca
    59341 aggcagtaaa cccgactaaa cgagaaggtc ggtccatctt ttcttttaat tcttgaacga
    59401 tggtatcaac gatcagatat ttgatcagta acccacctaa tgccaatgca agtaaaatca
    59461 aactagcaga acctaacata ctcatgatcc cgcctcgaga aagcagttca tctagctttg
    59521 gatcaggtgt atttgcgaca taaccattca tcaagatgtc tgctgctttt ccgaccgtca
    59581 aacttttctc atggataaaa gaaagaacga gagcagctgt cgatcccgct agtaatgtag
    59641 ggatagccgg aatttttttg attgcccccc caaataaaat aactagtgga atcaatgtcc
    59701 aaggagagat ccaaaagctc gctttcatgt tttggatcat ttcattgata ctagctagat
    59761 cggctgaatg actgttatgt cctaatatcg tataaaaaat caacgaaagg acgaatgctg
    59821 ggaaatccgt ataacgcata ccagcgatat gttcgaatag attaactcct ccaatacccg
    59881 ctgccaagtt ggtcgtatct gataaaggcg agcagttatt ccctaaaaag gcaccggaaa
    59941 cgactgcgcc tgtagtcatt gcatcattga atccaagtaa atgaccgatc cccaaaaaag
    60001 caattccaat cgtcgaaatc gttgtaaatg aactgccgac agtcactccg acaattgcac
    60061 aaacaagaaa tgctgtcggt aaaaagaaac gcacagaaac aagtttaaat ccataaacca
    60121 taatcgtagg gatggttcca gataaaatcc atgaagcaac taagacacca atcaaaagaa
    60181 aaatcaggat tggaatgatc cctggagtga ttcctttgat gatcccgtca tggatctcat
    60241 cccatgtgaa ctttttaaat ctgccataaa acaacaaaca catgaaagct gcaaggatcg
    60301 ggatatgtgg cgagagctgg aatccgatga tcagtactcc taggagtgcc aacaacacga
    60361 tcaatacagc gatactttct tttaaagaaa tatttttatt catcgtcgat ctcctatttc
    60421 aaatgaaaag gccctgccat gaaaaaacta ttcacttggc aggacctcct actaaatcag
    60481 cgacaaaacg tcggctgaaa gacttatttt tttatttttc ttctaaactt ttttctacct
    60541 caatccccca agaatctttc ttgaagtgat tgatgatcaa tggaataacg aacatgacca
    60601 ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc tgtacctaat gtagcaaata
    60661 cgacaacgat gatagagaag atcagcatgc agctgactac ccaagctaat ccgtttccgt
    60721 ttttaccaat tcggtaagga cgttcgaatt ctggatgttt cttacgcatt ttaagcaatg
    60781 aaacagcaat caaaatgtac acgatacaat aaataacagt tgtagcattt gtaagtgtca
    60841 agaatacagc acttacgtca tccattacac cataaagcaa agcaaataat gaaataacga
    60901 ttgattgtgt tagaacgaca tttctagata cgccgtattt gttttcttta tggaatccga
    60961 attttggtgg aaggaatcct tcacgagcta cttggatgat tgtttttgat ggtccagtaa
    61021 cccaagctga taattgtaaa agcacaccaa taaataccat gaagctgaag atatttccga
    61081 tcacttctgg taagcctagg atttgacagt caatcaagat aggctgcgtg atatttgcta
    61141 attccatttt tccatctggt acgacatttg ctacaagcat cgcattgatg acattcaata
    61201 aaactaatcc gatcaaagcg ataaatacgc cttttgtata attttttgtt gcgtctttca
    61261 aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa aataaatgtg ataccagcta
    61321 agtatttcaa tgaatcaata tgttccaagt cagggaaagc ttttgaccat gaaaagtcac
    61381 ctagatagcc gtttgagact agtccgactt tgaaggctgc caaaacacct aaaacgaaca
    61441 tgatgactac tgggatatat acccctagcc atacaccgat gttcccgccg acttttgcca
    61501 tatcgaattt caagttcaag atcgtgatga tccagtagat aactaaaata caacctaaaa
    61561 cgaaccagtg attattgcca agttctacat taccaaatgt gtttcctaac aatggtccta
    61621 aagtagaagc gaccattacc atacctggga acatttgcac ccataatagc catgcaacga
    61681 caaatcccca tttgctgccc agtgctgttt ttacccataa ctgaggaccg ccttcttgag
    61741 gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg accggcaaaa aacaaaactg
    61801 caaaaattga ataaaaaatc aatgtccaac cgactgcggc taacgtagga atactccgaa
    61861 ccgttgcaca cagtgccatt gtcagaccga taaaggtccc aagacccatt ttacttactt
    61921 cagatttact caccgttgac actctccttg attttttgga taaaataacg gtgtctttga
    61981 agtagtttca gcaataaacg aaacaattca gcgaatattt gaggggattc tttgagttta
    62041 ttgcttgaag ggtttcttca aagacaacct caaaacaaag ggatcgaaga gcttaggtct
    62101 ccccttgctc ttcagatcca aacatgcgtc agaaacagtt agtattcaag ctgttttcta
    62161 acatgattac cttgcttatt ttgcttcgct tgccaataat tggtctgcgt agattttttc
    62221 caatttttct tgaagagctg ctttgatttt ttcagcatat tcttcaaagt gttcttcttt
    62281 gttcatgtat ggtgtcatta cgctggcacg caatacagtt acttttccgg cacggttcca
    62341 ttcttcatct gagaatccta gttggttcac aaattgtaat gggctgttgc catagtctgg
    62401 gatagcaaag tcagtatgtg aagttaagaa ctcattaccg taaatgcttc ctttcacgta
    62461 tgaagaataa tcatatacgt catggttcaa tttattcatt gcaactaagt catcgttgcc
    62521 tttttctttg aatacatagt ctaccatatt gaaatctgga taagttaatg gatgaacttc
    62581 gatttctttg tctcctactt taaatgacaa gtcattcaag aagttgtaga aacgatgtgc
    62641 accttcgatt gatgcaccca ttaatttacc atatcctgtg acattcaaag gtaagacatg
    62701 gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt gaaccttcca agatataagc
    62761 cccaagtaat gctgggatat ctgcgccttt ttcaaataca tatgtcgcaa agtaagagat
    62821 aacgtctctc atgcggatat ctttgatgac gataccaccg gcagaatatg gaacatagcc
    62881 cattttatgt gggtcaatcg ttacagattc tgcttcttcg attgctttat atgcactgtg
    62941 tacttcttct aagatataat ctttgttttc tgtaaagaca ttgtatttgt aatgaacatc
    63001 ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct aagaaaatcg cacggccgta
    63061 tccgccataa gcagcatcta cgtgtaagta gaagtaaata ccgtcttttt ctaatacgcg
    63121 acgcaatgca acgattttat cgataccgtc gattgctcct tcttctgttg atcctacaac
    63181 accgactaca cctaggattg gtgttttttc agctgctaga ccacgaacga ttttttcaag
    63241 ttcgttgatg tccatgcgat agttgtggtc aactggcact ggaatcactt ggtctaaacc
    63301 gataccgatg atgtcggcag cttttagcca tgaatagtgt ttagtttgtg gaaccaacca
    63361 ttttcctaat ttttctaaat gttttccgct acgtgctgag tgagctttga tttcgtcgat
    63421 tttttctggg acgctgtcta acagattcat gatttcttct gttgacaagt tcattaattc
    63481 ccaatcgctt tttccagcaa ctagttcagg tgtcacttct ttcattgcaa gcggcaatga
    63541 tttgatgttg cgggcatacc aaagtccttc taagtttgct aaagaaccat cagcaacgat
    63601 gtggccccag ccatctttat agctcattaa ttttgcaaat tccattccta cttcttcttc
    63661 catttggctt gttgcaggag aagattcata ggcaacgtta ttaccattcc atagcatagc
    63721 aaaattgtac gctagtaaag atggcatcaa tgtttcagag ttcatatgac cccagtaacg
    63781 tccggcatta tgccaaggaa cggaatgggt acgcatccgc gctgaaattt ctgatagaac
    63841 atcctttgtt ttattcacag tgtgttcaaa actagcactg cttttttctt ctggcgtaat
    63901 aataggcatg tcttgaggca tgtagttttg acgccagcct aaatgttcat ccaccaattc
    63961 attcaacaaa gctttataga tttggccgtt ctcagctttg tccccaatga atagggcatt
    64021 taggtttaga tctttcgaca atgattcact catagtgaaa ccctcctaaa aaaatattta
    64081 aattttgtcg agccctacat tacacctgtt cttctattct gtaaactaag ttttggcctg
    64141 atttttaatc atattttaat atgtatcaat tatccgaatt gttcacactt ttgaaataat
    64201 taaaaaaaac gagggaattt acaaatgtaa actccctcgc aaagattgta ttaacgacaa
    64261 ccagcgttct attttaggat gggtttcaaa attggaatgt tgtaatagta tttccctttt
    64321 tcaaggtaaa aacttactaa attctaatca attactttgg ccaaaaagta atttttctta
    64381 ccttttcgaa taaccacaaa cttaccatcg aattcatcgg atggatttat gacaaaattt
    64441 aaatcggtaa cgcggtcacc gttaatactt atagctccgt ttgaaacatc ttcacgtgct
    64501 tgacgtttag atggttcaat ttttgtggaa acaagcagtt ccacgatatt ttcaggtgtg
    64561 cttgaaattt caacgttcgg cattttaccg aatccttgcg cgatttcttc cgcatttagt
    64621 tctttaatat ttccagagaa caatgcttgc gttatttttt gcgcttcttt caagtcttct
    64681 tcactatgca caaatcttgt aacttcttct gccaaacggc gttgtgcttc acgtttctca
    64741 ggttctgtct ctactttttt cgctaagtct tcgatttctt cttgagacaa gaaagtaaag
    64801 aatttcaagt atttcactac atcgcgatca tcttgattca accagaattg gtagaactca
    64861 aatggtgaag ttttcttcgg atcaagccaa acggcaccgc ctgcagtttt tccgaatttc
    64921 gttccgtcag ccttcagcat caatgggatc gttaaaccga acgcttctgc ttctggtcct
    64981 tctaattttc ggattaggtc taatccagct gtgatatttc cccattggtc tgctccaccg
    65041 atttgcaatt gcacatcgta tgttttatgc agatgcaaaa agtcgataga ttgcaggatt
    65101 tggtaagtaa actctgtaaa tgagattcct acttccaaac ggctggcaac gatatctttt
    65161 gctaacatcg tgttgatgtt gaagttttta ccgtagtctc tcaaaaattc aagtaaagag
    65221 atttttgaca accagtcgta gttgttcaca aatgtgatat ttgcatcttt accaaacaat
    65281 tttctcattt gattggaaag tgcgtccacg ttatgttgca cttgttccat cgtttgcaac
    65341 acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc ctgttccgcc gccaatcaga
    65401 atatatggat gatggcctgc caattggaat cttttcatca tcataaatgg aatcaaatgt
    65461 ccgatatgca tcgaatcacc agttggatca acgccgcaat aaagtgaaat gctcttttct
    65521 tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat taatggcgtc gcgccaagtt
    65581 aattcatcaa taatattcat tttactgacc tccgtgtagt ttttttatga aagcaggaac
    65641 ctgctagcat atgtaattaa ttatttcttc aggataggat aggaaataaa aaaagtccct
    65701 tggcatataa gccaagggac gaaatctcgc ggtaccaccc aatttgtatg tctagatatc
    65761 caacatacct ctcgaacatt taacgctgtc gcgtggcaat cactgccaaa gactcctgaa
    65821 atgtaattcg ctttaaagca tgtactggtt tgcagcgacc accagctttc tgaaacattg
    65881 gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt attaatatgt gtctatcata
    65941 gagcgcaact ttttctttgt caacagaaaa aggatatttt ttttaaattc ctcatttttt
    66001 cttttttgaa tccgctctaa ttccgaacat tttattttca attattgtta aaagaaataa
    66061 ctttttctgt attttttctg caatttatcg agaagtcact ttactttgcc tcttattctt
    66121 tcttcgttac aataaggaat gggacaagta aaaaaatgaa aaagcaaggg acgaggtata
    66181 tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc tttaactatc gttattttaa
    66241 ttctaagttt gttttttgtc atccgatcat ctacaaaaaa agaaccaatc attcctgaaa
    66301 aaaaaataac agaaaaagca aaagaaccga cagatgataa gcaacaacta gttactcaag
    66361 caaaaaatct ggctcaaagc tacgattatg accaagcgat tgcgttattg gaaaaagata
    66421 gtaagaacgg atctactgaa acacaacagc ttcttcaaac actaaaaaag gagaaagaac
    66481 aactgatcga ctggtctgat cctactcaga tctctcatgt ctttttccac agcttgatcg
    66541 tggacccgca aaaagccttc cactctcaac aggcacaagg atacaaagat tatatggtga
    66601 ctgttgaaga attcaaccgt tcgatcgaac aactttatca aaatgatttt gttttagtta
    66661 atttgaatga tttgatccaa aaagacgaac aaggaaatct ttcctttaac gggcttgcgc
    66721 tacctgaagg aaaaaaacca ttggttcttt cacaagatga tgtcagctat tatgaatata
    66781 tggataattc cggttttcct agccgattgg tcgtatcaaa taacaatgaa atcaaaaatg
    66841 tctataaaga gaaagataaa gaagaaatcg gtgactttga catggttcct ctgatcgatg
    66901 cctttgtcaa gaaacatcct gatttttctt atcgaggcgc aaaaggtacc ttggccctga
    66961 caggttacaa cggagtttta ggttatcgaa catcaaaatc tcaatatgga gacaatgaaa
    67021 aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga acagttgaag aaagacggct
    67081 ggagctttgc ttcccataca tggggtcact tgaatatgac tcaagcttca ctagaagatg
    67141 taaaaaaaga caatgaattg tggcagcaag aagttgcacc tatcctagga aaaacaaata
    67201 ttctgattta tccatttggt gcagacatca gcgactggca gccttattca gcagaaaatc
    67261 aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc gatgcttcta
    67321 caccagcttg gggacaatta ggtacggatt tctatcgaaa tgctcggatc aatatcgatg
    67381 gcattcgttt tcaatctgat ttagacggaa aaaatcctat tcttgatcca ttcatcaatg
    67441 tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg attttacgtc atttatgaac
    67501 caaaaaagcc gaactgacat tttatcgtca gtttcggctt ttttatgctt tatttcttct
    67561 tcggtgcgat tcccttgatc aggacttctg tccattctgc tgtatatgga agcagttttg
    67621 cgtatatctc cggcttagaa attccttcta ccattgcttg aaaatagagg atcaagaact
    67681 catccgaata tttcaaatcc acttttcctt ctttccgccc tcgatggaaa agatcaagca
    67741 tgatactgaa actttcttgc gtatacgcat ccatcgcgtc agaaaattct tttttttctt
    67801 tttttgtata gtatgtcatc aaatcgatat aaaattgttt gttcactcga ttgagataac
    67861 ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa tggaagattt tccgcaatca
    67921 acgctttcgc ttcttgccct aaatcattca taaatgcaaa aaatacttca taaatcagcc
    67981 cttccttgct gccaaaatat ttaaaaatcg tcgctttact tacctgagcg ttctcagcta
    68041 ccttatccat tgtcactcca gcggttccta tgtctgtgtt catcaagtca aaagctgctt
    68101 gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa accgttcatt tgtagagcct
    68161 cccgtaattt taaactttta taatatatat agttcaaatt tattctgagg tttattataa
    68221 ctatctattg agaaagtatc aagctagcaa ttccttgttt atgaattcat tgacagaaat
    68281 ttataaaaag aatattatgt actaaaataa taaaaaaagt tttataaaaa ggtataaaac
    68341 ctttttatta aactatttaa tcttgtttag tatatatttt tagaaaaagg agtggggttt
    68401 ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag ctttggtcgt ttcgaagctt
    68461 taaaagatgt ctctttcacc gtacattcag gtgaagtcgt tggatttatc ggaccaaatg
    68521 gttctggaaa atccacgacg attcggacac ttttagggat tataaaaaaa gacggcggaa
    68581 cggcaacgat ttttgggcaa gatgtatggg aagatagttt atctatccat gaacgtcttt
    68641 cttatgttcc aggcgatatc tctttgtggg gaaatttgac tggaggagcg atcatcgatc
    68701 tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa agatgaactg attcaacgtt
    68761 tctctcttga tcctaagaaa aaagcaaaaa actattcaaa aggaaatcgc caaaaagtgg
    68821 gcttgattgc tgctttggct gttgattcgg atctctattt atttgatgag cctacttctg
    68881 gtttagatcc tttgatggaa acgattttcc aagaagaagt tgaaaaattg aaagaacagg
    68941 gcaaatccat cctactttct tctcatattc tcagtgaagt cgagcgacta gcggataaag
    69001 tcgtgatcat acgcgaagga acactcgatg aagaaggaac actcgatgaa ttgcgtcatt
    69061 tgactcgttc gaccatcact atagaaacag aagcagagaa tagtcactta gcacaattga
    69121 acggtgtcca tgactttgtg caaaaaggtc acctcgctca atttgctgct gataacgaag
    69181 ctttcgatgt gattttagct gaagcgtcca aattaggtgt gaaaaaattt gaatctattc
    69241 caccgactct tgaggattta ttcatgagac attatgaagg atgaggagga agcgaaatga
    69301 ctgataaatt ttcacgctgg tccacccttt tttctcaaaa ccttagccgt gactggaaaa
    69361 agatcctgat atggctagtc ggtgtcactg ctttttctgg tggttttgtc cctgcgtttg
    69421 aagaattgac ttccggacaa ggattagctg gattatacga aaccttgcaa aacccggcaa
    69481 tgattgcaat ggtcggacca actccagctt cgaatgctgc tgagtatact ttaggagcga
    69541 tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc tgctgtgatc tctgttttac
    69601 acgtggtgag ccaaacgaga aaaaaagaag aacttggtct gattgaactt attcgttcct
    69661 ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc tgaaagtatt ctgatcaatt
    69721 tgcttttagc aatatcgatc agctgtacga tgattttttt ccaagcggat tcgatcactg
    69781 cagaaggtgc ttggcttttt gggctatctg taggtagtat ggggattttg gggagtgccg
    69841 ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag ttcgaatggt ctgtctttag
    69901 cgtttttagg tggactttac cttctacgag caataacaga tgtagctgat tcctcccttt
    69961 cgatgtggaa tcctttaggc tggtcttatt tgacctatcc atttacaact aacaactgga
    70021 ttcctttgct ttatttgttt ttgttcagtc taatcttgat aatcagttca tttgttttag
    70081 aggaacatcg agatttgaac accggctata tccccgaacg tgaaggaaaa ccgtatgcta
    70141 aacgttctct tttatccttg cgtggtttat tattctatct aaataaaggg atgacaatca
    70201 gttggcttat cacttttggt ttgatggggg cggcatacgg ctctatctac gggaatatgc
    70261 aacagttttt agaaagtaac gacttaatga aacagatgtt tatccaatct ggcgtctcaa
    70321 ttgaaaaaaa ctttacaagc acgatcatgg tagtcttgat ttgtctagct gctattttac
    70381 caattgctag catcaatcgt ttatttacag aagaaaccca cggacgtatg aatcagctgt
    70441 tcgcaacaaa aatttctagg aaccaacttt attggaccaa tatgttacta gctattctat
    70501 ttgctatatt ggcaatcctg attagttctg gtgcactagg gctttctgct ttggcttcga
    70561 tgaaggatgc gaaagacatg gatctattga tgtttcttaa agccgggttc aatttcctgc
    70621 ctgccgtttt agtgtttatc ggaattagtg gctttctatt cggatggctt cctcgaatcg
    70681 gtaaacttgc ttatatctac ttagcttact cctttgcact caattacttt ggcggaattt
    70741 tggatttacc agaatggttt tccaaaacag ctgtccctag ctgggttcca cggatgccaa
    70801 acgattcttt tgatctttcc gtgttcctta cgttatcatg tatcagcctt gcactgttcg
    70861 tccttggttc tattggttat aggcgaagag acttactaga aggataataa aaaagcatgt
    70921 acaatttcat gctagcctcc aaatctgggt caaaaatcca acatttggag gtcatttttt
    70981 tatttacaag tcttctaact gctctttatc aatgatccag acttctcgat tctttctttt
    71041 tacataacca ttctcctcta ataatttaaa ttttctagat aaagtttcgg gggtcgtacc
    71101 gataaatgca gcaagttctt tcattttcat tggcaggaaa aagtgatcct tccctgtcgc
    71161 tttagctaag tcaagcagat agacagcgac tcgttcttct actttttcca tcatcaaaaa
    71221 agttgtctgc tgctgtactt ttgttaattt ctcaacgttg attgctaaaa gtttcagact
    71281 aagggcggga taatgcagta agagttccga gaaatctttt tgtctcaata tgcaaatttc
    71341 cgttttttct agtgcttcgc caaataaaga ctcatttgga acgcctagca actggctctc
    71401 tccctcatat ccgcccggct ctattactcg taaaagctgt tcctttccgc tagcagataa
    71461 ttgatacact ttcatactgc cggaggccac gatcactaat tgaggttctc cattcggcgc
    71521 aagaatctgt tctccttttt ccacatagtg atgtttgaca agtgcgtgta ctttttcttg
    71581 atcttgctgt actaagtgat tgaacaaagg aacaaatgaa acacataaat gatcatgact
    71641 cataatctct cctccttcta aacagctatt tctaataaag aaacataact tcccttattc
    71701 caactgataa ttttctttta tcatagcata aacgtcaagc gaaccagata ccagcaaata
    71761 gagtggattt ctttttgtcc cttgctttat tttccttctt ttgagacctt aagtaattga
    71821 ttcctatcaa ttttttaaat atttactttt tgaataatca aaaacaaacc ttcactcgca
    71881 agccgagagt aaggtttgtt tttcgcattg cttatttcgt gttgtctatt gaaatttttc
    71941 cgcacctagc ttttgatata gtggtgctag ttcttgtaag gtttcagcgg ttatttgctc
    72001 aacgtgttcg atttcttgcg gatagaagaa tagtttaccc atttgaaagg ttcggtctct
    72061 catcgctttt ttcaactctt cttgtttctt tgtatcggac aaatagtcag acaaagctaa
    72121 gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa taatccttga tatcgactgt
    72181 tttgctccac tctttcagta tcgcaagcaa ctggttatac gctgcaatgc taccatatcg
    72241 ttcttcgctt ttataatgtt ggtacatcaa atagatttgg tattgttttt gattcaacaa
    72301 aactgctaga caggcattct cgtcttgacg agattcgctt cggtacgcgc tccaaaaatg
    72361 actgcgtaaa ttccagccat tcgtccagct ttcgatttta ggtttgctca tgccgtacat
    72421 atcaggcaaa agtgcagctg tctggagctg aatctctttc catttttgcc agtgttcttt
    72481 atattctttc ttgatttgtt caatcttatc tgttggatgg ctctctttga gctgtttgaa
    72541 tgcgtagatt ggtcgatcaa acaactgata atctgatttt ccaagttcca ttgtttcgcc
    72601 tctttccgtt atcttttgta ttctacctga attttgcgat atggacaaat agaggttgtg
    72661 aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca aaaaactgcg atgagcatca
    72721 ccgcagtttt attgaactta aggttgattg atcacatcta aaagcgttga aaataaaatg
    72781 gcactagaag cagctcctgg gtcgatatgt ccgattgcac gctctcctag ataggaagct
    72841 cgtccttttt tagctaccaa gactttggtg tgttcttttg cctggtcaat gatttctttt
    72901 gttaaattcc ctgccttcaa tgcctctgtg actggcagcc aaacatctaa catcgtttta
    72961 tcgtcagctt ctgcctttcc tcggctttga atacccgctg ttccctgctc gatcacttcg
    73021 cctaattttt cttgagaatc aatagaatcg acctcttttg tcgctttcat catgttcatg
    73081 aatgcagatc cgtataacgg accagaagcc ccaccgactt tcgatatcat cgccatagag
    73141 aatactttga atgtatcgct gattgtttca ggatgatcgg cttgaaaagc ttcttcataa
    73201 gctgaaaccc ctcttcccat gttatttcca tggtctccat cgccaatcgg ggtatctaga
    73261 tcactgagat attgtttgtt ctgattgatt gcctctgcaa attgcgtgag ccattcttgg
    73321 atttcttgaa cggttagttt catattatca cccttttatc ttgagatttt ttaccatgaa
    73381 atcgtctgta cattgctttc taaagaatct ttccattgtg tctccgtcaa gtcgatcagt
    73441 gttagagaaa gaccttgcat atctagtgag gtcataaaat taccgacttt tcggaaaact
    73501 acattgacgc ctctttcttc caacagagct aaaacatcat tcatgaacac gaactgttcc
    73561 atcaaaggcg tcccgcccat tccgttgact agtacacctg cttcttttgg taaattcgag
    73621 taatcatcta aaatcttagt aacaagttcc tttgccagta ctttagaagg ctgcatcttt
    73681 tctcgacgat agcctggctc accgtggata ccaacaccat actcgatctc atcttctcct
    73741 agttcgaatc ctggtttccc tacttctggt acagttgctg ctcgaagcgc tagaccgatc
    73801 gtcttagttg cctgtacgac cttttctccc agttctttca actcagctag ggaagcaccg
    73861 tttcttgctg catcgcccac gattttgtgt acaaggacag tacctgcaac tccgcgtttt
    73921 ccagcagtat acgtactatt ttcaacggcg atatcatcat ctacgatcac actttctact
    73981 tcaatatcgt ccatagcagc taattccttt gccatatcga agttcaatgc atcccctgta
    74041 taattcttaa cgatcagcaa aactcctttt ccactatcgg cttccttaat agccgtctga
    74101 atctgatctg gagttggaga agtaaacaca tcaccaagca ccgctgcact taacatccct
    74161 tcccctacaa acccggcatg ggatggttca tgtccgcttc ctccaccaga aaccaaacca
    74221 acttgttccg ctttctgatt cttggctaca acacgggaag attctacacg atgaacaagt
    74281 tctggatagc tttttactaa tccttccagc atttcttcga caatattgct tggatcattc
    74341 atgatttttt tcaatttatt cccatccttt ctttccggca ttttgtgctt ctgttaagat
    74401 ttgctgagta tcatctgtta taccggcagt gatggctgct gcaaaagctc cttccactaa
    74461 aggcgcatcc accagatgat aacgttgctg ctgctcttct tccaacatgt cttttgccag
    74521 ttccgagttc aataccgcgc taccgatatc cgcaaaaaca aaataactag ctgcatcgct
    74581 tgattcattg actgcttcta cgattttcat cgggtctgat ccgatctcgc catttattcc
    74641 gccgagagaa aaaatttgca cctcttcgga tttttgcatc tgctcaatca tttctttaat
    74701 accatctgtt actttttgac tatgagaaat aagcaaaata ctttttttca caagaatctt
    74761 cctttctttt acatagttgc taacgattgg tttcgctttc attaatgaac aattcaagta
    74821 tataataaat ttctaatttt gggaaaatat gtgtagtgct tcttgaagtt tgttatactt
    74881 taattaaaag agaggagtga tcgaatgatt acaacaacaa cgaacagtgt agagggaaaa
    74941 gccgtagtag cttataaagg gatcgttttt ggtgaggtca ttactgggat ccatgcattt
    75001 aaagacttgg aggcggggtt gcgaaatatc tttggcggcc gctcaaaaag ctatgaaaac
    75061 gaactaatgg gtgcaagaga agaagcactt gccgaaatgg ccgaacgtgc aaaagcatta
    75121 ggagctgatg cgatcattgg cgtaaaaatg gactatgaag tattaggcag cgacaatggc
    75181 atgctgatgg tcacatgtag tggaacagct gtctccacga gataagtagg ggacatctgt
    75241 tttaataact aaagagagat gggacatgta gtggcactca agaattagat ttttcagtct
    75301 aacttcgagg tactctacct tcccatcctc tcttttttat ttctttttgc ggatgattcc
    75361 gtaagctgct gcgactaaca cacccgcccc tgctaaggaa taactgacag aagctgtttt
    75421 ttcatttgtt tttggcaaag tctgacttgt tgatttggat ggctctgtcg tttgctgaac
    75481 tgtttctgtc gtgcttccag aggtcactga tttttctcct gccgaatgca aaatatcttt
    75541 tattcgttgg aaactagctg ctaagtcagc tttttttgct tcaaaatcat tagcaactgt
    75601 ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc gctgtgatat ccgcttgagt
    75661 ttgacttagc tttccagaag ctgtttgagc aacctgattt aattgctgat tgaattcttt
    75721 tgcttgttgg atcttcccgt caagatctgt ttggacagct tccgcttttg ctttcgcttc
    75781 ggcaagcttt ttctcaagtt gtgcacggtc atttcccact ttatttttaa gatcagctaa
    75841 atcttgatcg attgctgctt ggtcttcttt caatttgttt tttgcagctt ctaatgcttc
    75901 ttcgagttct tttcttttat tttccaagtc ttgcaaatga tcgatttggt cagaagaggt
    75961 agaactattt attgatgtat cttctgaaga tgaaatcgtt tctcctgaat tatctgtcgg
    76021 tacattggaa gtcatttctg tttccgaggc accgttttca gcaccagaag agcttatggt
    76081 tgttgaaggt tctgtttgag caggttcaga agtttccgca aaagctgtct gtcccaataa
    76141 gaaaatactg gaaacagcaa ttggccagat taacggcttc attctttttt tcatcacttt
    76201 tcccatcctt actacttttt aactaatttc ttattataag caaaaatgtt tggaagaacc
    76261 ataaacacaa caggctgttt gtctaaaaaa cggtgcatct tttatttttt tgtacgttcc
    76321 gggcttttcc atgtcccgct tccataacct aaaatcgttt tgactgctgg aatgaaagtc
    76381 gtcacgatcg ttatcgcgtt cactatccag aacaaaagca tatatagtgg tgcgaaaaga
    76441 aggtatttca attttcttcg tcgatcatct acaatcagcg aagctagcaa ctggaaaaat
    76501 cctgcgacca tttcaaagca gatgaacaca aaactgaatg taaaagtaat ataaatctgt
    76561 tcgtagtttc cttgatatac ataatgcccg attaatccag caaaaagaac tacggaaata
    76621 caaaagaata ttgaccagac aatactcaat gtttgatcaa tgaagatgat cgttcttcca
    76681 atatgtttaa aaggatggag catcactttt ttgaaattcg tcagccagac ttctgttcca
    76741 ccttttgccc aacgttttct gtgtcgatat agcatcttca acgtcacagg tacttccatg
    76801 aaaaagataa tctcaggagc aaaaacagat aaccagtcat ttaactgatg atcccaagca
    76861 atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc ccacatcgat caaggcttct
    76921 tttcgataca tcgtatttgc tccactgtac gcataaagac caccgaaaac agctgactgt
    76981 gttcgtttaa tgatgcccac gatacttgag aattcaaccg tttgagattt tgcaatcaat
    77041 ttggtacgat tttggacatc catatttgca gtgaccgcag caatatgtct tgcaccaggg
    77101 cgaatgaaat aattcacata cctaatcagt gcatctggct ccggtaccgt gtctgcatca
    77161 ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga tattaaacgc gtgggctttt
    77221 cctttatttt tttcgatccg aaccacacga agattagcgt attttttcat cagtctagct
    77281 aatatttctg gtgtttggtc cgttgaacca tcgtctgtca ctaatacctc atagttgtga
    77341 taattcaatt tcgtcatcaa atactcgatt gtatattcga tcacaatttc ttcattatgg
    77401 gctggcacca tgatcgtgat gaacggttcg acactcaagg gaacatctac ccattctttt
    77461 tgcttatgtt tgtataaaaa cacataacac cagacaccaa taaaccatac aaatccgcct
    77521 aaaatcggat aagaaacgag aaaaaaatac ataatagaca aacaagtatc taagaataaa
    77581 ttacctgtat gggtgattga gatcatttca gttccacccc attttcttta tacaaatctg
    77641 caatgaatgt cgtatctaga tttttctctt cggagacaga ataaaaacgg accgtttctc
    77701 tctcttcttt tgttccaaaa cgttccgtat acacttcttc aagtaattct tgtctttttt
    77761 tcaatttttc atcttcatat tcaaaagatt tatttacttt tttgtataga tttcgattgt
    77821 tccacctagt caatacaaat gaggtaatca ggaaactgaa aaaaatcaat acaaaaaagc
    77881 cgattaagaa atacagcatt ttcttttctg ataaaaaagc gtacaaggaa aaatccttgg
    77941 ctctttctgg tctcgttaat gggaaaaaga cccatataaa aggaagaaca acgatcatcc
    78001 atcctaaaag agccactaac gtttgccaaa tctttaacca gtaattacct tttgaaaaat
    78061 acttatcttt caccaaaatt gatgcaactt cttctcttgt tacttttttc atatcctcac
    78121 ctcacgtttt tttgttacga cgtaggagtt taccatacat ttcatttctt ttcacataaa
    78181 gtgttcctaa atttttccta agaaaaagaa caaaacattt ctcatcagca aatgaaaaat
    78241 attttgttct ttttatcagt tgttcttttt attttttatc tcttaattat gttgctcttt
    78301 ccaatacgac aatgcttttc attcctctag atatcgccgt gtacatttgt ctgcgtcttc
    78361 tttgctcatc tccatcgttt tcaaatggat ataagattac atgatcaaat tccagtcctt
    78421 ttgctagact caaaggaatg atttggcagt tttctaatcc atatgaattt tttttaatcg
    78481 attcttgtag cgtttgggtt tcctcttctg tttgggtgat aactgccgct ttttctcctt
    78541 ttagagaaac caaaatctca gctagtattt tcaacaattc ttcagaggat cgaaaagagc
    78601 ggtattcggg ttcttttcct tgcggtcgga tcgatacgat gtcgatcttc tcttgatcca
    78661 cagcataggt tttgaaaagc tctgtgattg ctccgctaga acggtaacta tttctaagat
    78721 cataccgatg gatcggcaga tggtagtcgt cgaaacaacg cataatatca gcaaaagtag
    78781 tggaagaatt aaagattgcc tgattctcgt cacctactaa agtaaagtga gtttttggaa
    78841 ataattcgat caatagacct aattgtgcca gggtataatc ttgtacttca tcaatcaata
    78901 gatagcgaaa tgctggtaca tttacctttt caacaagtaa atgacgaata gctaatataa
    78961 tgactgcttc atcaacagta cgtgtgggtt gccaagcata agctgagcca tatcgttttt
    79021 cataaattct ttcaaacaac tgatgctcat ccacccatgc catttcttcg atttgtctgg
    79081 ttatttttcg atattttttt tgtaaaagtt tccttgcata agctacaata ctttgttccg
    79141 aatcatcaga aatcaattta ccgaataatt ccaactgcct atcttctgtc aacgagagta
    79201 cctgattctg cacagcagga ctttttgctt gtttcaaaag atgattcttc cattcgctca
    79261 tcagtgcctt tttcgttgct tgaagcctat cgatcaattt aggatgagca ggaactttct
    79321 catatatttt ttcgatcgtt tttttagaaa ggactgtttt tccattttta gtaagtccac
    79381 ggataaaatt cggatgatct aaaaacaaag gatctgattg ttttagatta tcgataaact
    79441 ttttgctacg caaacgttct gtctgctccg atacattttc tcccgtgata cgtttgaaat
    79501 aagcctcttc accttctatt tcctctgctg aaagctgtga aaccagctgt atgatggtta
    79561 gattcagtgg atttctttca cctaatgatg gtaggacatt cgaaatatag tcgataaacc
    79621 gatgattagg tgatagaatc agcaagtcat cggatgtcat cgtatctcga tataaataga
    79681 gcagatacgc aatacgctgc atgatagcag aggttttacc acttcctgct acgccattta
    79741 ccaagatatt ttgacttttc acatcccgaa tgattgtgtt ttgttccgat tggatcgtag
    79801 cagtgatagc cttcatttca ttggtctcat cttggcctaa gacttctaat aatacgtcat
    79861 cttgaatagc tacagacgta tcaaaaaact tgaccaaacg atctctttcc aaaagaaatt
    79921 gtcgtctttc tttaataacc gtatcgattt tattttgacg aaccgtataa gaagatggac
    79981 caatcatgtt attgtaaaaa agttcggcaa ttggtgaacg ccaatcataa acaagatttt
    80041 catctgcatc atctgtgaaa tttgctgtac cgatataaaa ggattctttt ggctcattat
    80101 ccagaaagtc tacaacgact tttccaaagt agggactttt caataaacgt tcgatctttg
    80161 ctagctgatt ttctgcgttt ttctgtttga tattcatctg atcgatttct ctatttttca
    80221 tttcaatcat cgaaaaagta tccaggttat ctaagtaact gtcaaaattc aatcgaagct
    80281 cgccagccat ttctttcaac gactggtttc cttctatttt tgccgtatcc atcgattccc
    80341 gcagttcgtt ttttttatca attagctttt gatacacgtt cttcagatat gcttcttcta
    80401 actgtttttg tgtgttgacc atacacattc tcctcttttt ttaaagtggt ggaaccgatt
    80461 agtttacctc ttttaagagt cgaatgcaag ctttttaacg agtatcattt gttaggatat
    80521 atcagttctt caggtcggac agtttgatat aaagaaaatg caccatcaag gttttttacg
    80581 tggtagccag actgcttcaa gattctttcc gctagatagc tacgtaatcc gctatgacag
    80641 ctgacgatat attcttgttg gctgtctaat tcatttaatc ggtcccgaag ttcattcaaa
    80701 gggatatgtt tggcttcctt gaatttgccc ttttccaatt cttctgctgt tcgcacatct
    80761 aataaaattt ttccttttgc tagttctgtc ggcaattcat gccattgtac ggtttcacta
    80821 agcccttctg caatgttcat tgctgcatat cctagcatgt ttactggatc tttggctgag
    80881 ccaaatggcg gtgcatacgt caattctaat tccggtaaat cgaagatcgt caaatgccct
    80941 ttgattgctg ttgctaaaat atcgatccgt ttatccacac cttttgcccc aacaccttgt
    81001 gcaccataaa tctcgccagt ctctggatgg aagatcaact tcagtaaaat atctgttgct
    81061 tctggataat agcttgcatg atcttttcct gatacatgaa tgacttcaaa agacagttcg
    81121 ttttctttgg ctgttctttc acttaaaccg gtggatgcgg cagtcattcc aaacgttcgt
    81181 ataattgctg ttccaatacc gccttgattt cttcgagaaa ttccggaaat cgtgtccgca
    81241 acttgtctac cttgacgatt ggctggtgaa gcaagagaaa taagtgcatc ttttccagtg
    81301 atttgctgtt ttacaacgat agcatccccc accgcataaa tatcaggaat attcgtttga
    81361 taacgttcgt ctactaggat acccccgcgt atgcctaatt cgattccagc ggtcactgct
    81421 agctggttat caggacgaac accaatggat aaaataacta aatcagcagg gatggtacgc
    81481 ccatcatcca gtcgcaatcg tttcccgtgg tcttcaaatc cagcgacagc atgagaagta
    81541 atcacttcta ctccttgatg agacaattct ttttctataa aagctgccat ttcttcatcc
    81601 agtgttggaa gaatatgtgg tgcttgttcg atcaccatga cttctaaacc tcttcttttt
    81661 aggttttctg ccatttccaa tccgatgaat cctgcaccaa cgatcacggc acgctttggc
    81721 tgtttttcta atgcatgcat cactttatcg atatctggta catttctaat agaaaaaaca
    81781 ttgtcggctt ctgttatccc tggaatcgat ggaacaactg gttttgcacc aggagataaa
    81841 accaatgcat cgtactgttc aatgtgtttt tgaccctcat gtacgacttc taccgttttg
    81901 ttttcgggaa agatggctgt cacttcatga tttggacgca catctaaaca aaaacgagcg
    81961 gataatgatt ctggcgtttg gacaagaaga ttttcccgtt cactgatttc tcccgaaaga
    82021 taataaggca agccgcaatt tgcaaaagaa acatacggtc ctttttcaaa aacaacgatt
    82081 tctgcatctt ccattaatcg gcgaagccgt gtagcagcag acattccacc tgctacacct
    82141 ccgacaataa cgattttcat cttatttccc tcctactgtt cgatcaaacc attgattcat
    82201 gccaccacgt acattcacta catcataacc agatttcttt aattctttag ctgcttgttt
    82261 actcctcatc cctgattgac aaatcacata gacggtcttt tctttgtctc catcatatct
    82321 gtttatactt tgtaatggaa catttttagc tccttttata tgcccgcccc ggtattctgc
    82381 aggtgttcta acatcaagta acgctggatt ttccaaaagc ttttccttta attctgggac
    82441 tgttatcgat tttattgatt gaaatagcaa attcattttc tgcacctcat ttacattttt
    82501 ataaatacct catggggtat attaaacagg agctttatta atatgtcaac cagaatgact
    82561 cttcattttt ttcactactt tttatatttg cataaaatac cgataagggg tattatctaa
    82621 ttataaaaat agaggagaat gtgttatgga gattgataat aaaaatatcc tgaaccgttt
    82681 gaaacgtaca gagggacaaa ttcgtggtat ccaaaaaatg atcgaagacg aaaaagaatg
    82741 tatggatgtc atcactcaat taagcgctgt tcgttctagt attgatcgcg taatgggcat
    82801 gatcgttgca gataatctga aaaattgctt tgagaaacct gatagtaatc cagaagaaca
    82861 agcggctaaa ctagaacaag cgatcaagat gattattaaa aagtaaagga atgatttcaa
    82921 tgaaagtcgt tttccatatt gatgaattag aaaaatggtc agaaacaggg aaaaacgtaa
    82981 aaaacctgat aaaagcttct ccagaaacga cgattgtcgt atctgtaaat ggcatcgcga
    83041 ttaccggtta tctagactct gccaatgcag aattcttaga tttgcaagga gtcgtttttc
    83101 acgcttgcgc caatgctatg cgtgctaatc atatttcaga aagttcttta cctgaacaag
    83161 taatcgttgt accagcaggt gtccttgatt tagttgaact gcaatcccaa ggttatgctt
    83221 atatcaagcc ttagtcagta gaggttgcga cagaagtgat cagcttcaag aaatttcggc
    83281 ttatttttcg aagaagctac ttctggaaaa ccgtgaatca aaggttgtga aaacgctgtc
    83341 ctgcatcaag taataagaac agccaaacaa aaatagcttc tcatattttt cgtttggttg
    83401 ggcttattac cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaatcag
    83461 cggtttgacc taagtattag agaaaaaaac gggaaaatag ctcctcatat tttgccgttt
    83521 tttgagctaa taccgaaggt cagctgattg aacaccgttt aaggaagaga ttgtgaaaaa
    83581 gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat ggatttctac atttttgcat
    83641 aaatttggct taataccgag aaactggctt ttgaataccg tttaaggaag aggttgtaaa
    83701 atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagctgct catattttgc
    83761 cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaccaa gaggttgtga
    83821 gcctaccatt taactccgaa tcacaagaaa taatttcaaa aacaacttct cctgtttttg
    83881 gttcggttgg cttattatcg agaagtagct actgggagtc tatttatcaa aaaaaggaat
    83941 gcgacatagg tcacacccct tttttatttt ctttgaaaca tctttcggta ctcgacagca
    84001 tacaagacaa aagattgtaa aagaccgtaa gtgatgacgg caaaaatgac tctcacccac
    84061 agcgttggta attctgggaa aagcagcaaa aggatcatgc caacatagat gacagcagtc
    84121 gctactttgc cataccactt cgctccactt aatttgatat ttttagatag cccgattaat
    84181 ccagctacaa acataaagcc ttcttttatc aagaatagac caaacaacag ccaaagccac
    84241 ggtcgatacc atgccataga caaaatcaga aaaagttgtg tcaatttatc tgcaaacgga
    84301 tcaagcacct tccctaagtc tgttacctga ttatatctcc gagcaatgac cccgtctaga
    84361 taatcagaca aacctgagac aacaatgacc gctcctgcta tataaaatga ctgtctcagc
    84421 acaaaataaa gataaatcgg cagcaaaaaa atacggaata tcgataaaag attcggaatc
    84481 gtttgtattt ctttacgcca gtctgtgctt aatttcattt tatcgcccta cctcctcagc
    84541 actcttttct ttttatttta gcttacacgg ggaataaaac caatgatttc actgttttta
    84601 tttttcttta cagacactga tattatgagc taatttgaga taatcacttg ctgttttcat
    84661 cgtttactct tctttcttcc tgtcgtttgc gcatttcttt tactagtact ttatgagctt
    84721 tttttgatcc tcgctctagg tcacgctgcc gtttacgtgt tttataatcg ataaacagac
    84781 tgtctaaatc gtaacctgtc gggtacagat cttctgctga acgcaccaga cgaacacgct
    84841 gacgaggcac gtggattatt tttttgtcta aatatacatc aatcgtatct tcacctatat
    84901 cttcataaac taacgcttct ttttggtatt cattgacaaa tatccgatca ccttttgcaa
    84961 agagctgacg ttctttttta ttctccttcc tatttgtaac tgctgtctta ggaagaaatt
    85021 cttttttctg tgtccgatag gctttttccg ccaagtaacg ctctgcttga tggatcaatt
    85081 tcatcgacat atccatttta tgagcaatcc acaaagcttg gctttctcct gtttcttcca
    85141 acccgcaact gatatttagg acgaagtgct tcccgatcga aagccatagc agccggcaca
    85201 aaatcttcat gatctccggc gaatttcttg atttctccat aatgggtcgt ggcaatgatc
    85261 aatgcccctt gctcatacat agattccatg attgcgatcg ctagtgcagc cccttcattt
    85321 ggctctgttc cactgccaat ctcatctaaa agtaccaacg tattcctttt tatctttttc
    85381 aaaatagcag cgatgttttg catatgacca gaaaatgtac tcaacgcatt ttctaaattt
    85441 tggtggtcac cgacatcgac gaaaatctca tcaaagacag caagctctgt cccttctttt
    85501 gccggaactt gcataccgaa catcgccatc aaagtcaata aaccgactgt cttcaacacg
    85561 actgttttgc ctccggcatt tgctccggtg ataatcaagc ctcgatagtc tttaccgatt
    85621 tcaacatcca atggtaccgc atgctcaggt aaaaatggat gtctgccttg cttgataatg
    85681 atatgttccg atttattgat tctaggtgtg cttccattga tttctcggct aaattttgcc
    85741 cgagcaaaga tgatatctaa caccgtgatc gtttcgatca ataaatcgat cacttcttcg
    85801 ttttctgcga tagcaccagt caatgccgcc aggacttgat attcttcact gatttcttca
    85861 gcttttaaca actgatagtg ttcattcaat ttagaaatca cagttggttc aataaaaacc
    85921 gtcgttcttt tatttgattc atcgataatc gttccatcta ttttattttt atagctggct
    85981 ttgataggaa tcgtatagta ttcgcctttt tgaacaatca ttgcttcttg gatcatttct
    86041 ttgtttttcg gatgacgcaa gaatttcagt aattttgact ggatttcttt ttcgattagt
    86101 tgtaattgtt tgcgtacttt acgcaaatta cgtgacgcgt cgtcactgac tttattattt
    86161 tttattttct ggtcaatcaa ctcttctaca tttatcaaat caggaagatg tttgctataa
    86221 gcaaacaaca agggtgtctg gtattgattc ttatcaaaaa actttgtgat catccgactg
    86281 cttctaagaa aatccgcata ttcgatcaaa tcggttggct gcagaatcaa cccttttttg
    86341 acttgttctg tcagtgcatc gatcctcggc aaccccataa atggaacatg ctgactgctt
    86401 tctaagatca agcgtgcttc tcttgtttct tcttgacgat ccaagacagt ttgtaggttt
    86461 gtcgagaccg tcatatcgct gattcgtttt ttgctatagt ttccaattgc tcttgcttgg
    86521 atttcctttt tgatccgatc aaattgtaat tgactaatca tttcgttgtc catattctct
    86581 ccttttcttg aagagatacc tgtcccaaaa taaataaggg cctagagata aataaaagga
    86641 tagaacacag cttaaaaaag tcatgtccta tccttattag aatacaaaga tattgttttc
    86701 ctgacaggta ccgtttcata agcacaccaa ataaactttg tttattggct attcgactta
    86761 ctgattagat accagctaca ctcacaaaac ataccccatt cttctaggtc ttatttattt
    86821 tccacttaac tatagcaaga cacattttgg ttgtcaaact ttagtatgtt ttatagattt
    86881 acaccattaa tgaatagaca cctaccgcga taattgctcc gatcattgga cctgcgattg
    86941 gtacccatga atatgcccaa tctgaagttc ctttattagc tattggcaat accgcatgtg
    87001 ctaatcgagg accaaagtcc cgagcagggt tgattgcata tcctgttggc cctcctaatg
    87061 aaagaccgat ggctaagatc aatccaccta cagcgaatac gttcgttcca ggtgcaaatt
    87121 cgttttgtcc aaaagctagc aacccaagaa ctaatacaaa tgtaccaatt aattctgtga
    87181 tcacatttgc tggataattc cgtatagcag gacctgttgc aaacgttcct aaaatcgcgc
    87241 tttcatcttt cgttgcattc caatgtggca aataagaaag ccaaacaaga atggcccctg
    87301 caaaagctcc taaaacttgc gcaatgatga aaggaacgac aagattccaa ctaattgccc
    87361 ctgtcatcgc catagctaga gagactgcag gattcaaatg agctgggccc atgaatccag
    87421 aaatatagac agccattgtt acagcgagtc cccaaccaaa tgcgatgaca atccagccag
    87481 aagcctgtgc tttacttttg tttaaattca cagccgcaca cacaccatct ccaagcaaaa
    87541 ctaggatcag tgtcccaata aactccccca tgatttgagt catatcactg ttcatttatt
    87601 tctccccctt tagttcccgc agatctgatt ctgcaatcaa tgcttctaaa cgtttagtct
    87661 gttgcgcctt ctcctcttct gtccattcaa aatatccagc aattgcatcg atcacaggtt
    87721 gcttgatctc atcaagctga tctcgttcaa acaacagatg atttgtacga cgaatgagat
    87781 aatcgcctgg ggccaaaacc atttcttctt ctaaaccata acgtaaccga gctgactcag
    87841 cgagactcaa accaggataa ggtgccattt cttttgctaa ttcaaagata cgtcgggcat
    87901 tagtcccgta aaaatcagcg atataagtag catcttcttc ctctaaaccg gctgctactc
    87961 cttcttttgc taattctgtc actgtttctt ctaatttagt tggatcgaag tttccacctg
    88021 aaatctgata ttttttagag tcgatttctt tcgtctctat tccgtattct tcttttaata
    88081 attggcgtat caatcgcaaa gctccttctg ccatcttacg gtaatcggtg attttcccac
    88141 cagataaagt cagcaaacca tctggttctc tttctaaaga actacctctg gaaatcgtag
    88201 aaggtgcttt ttcatcacgg ctgttttcca aatggttcaa cacgtcttct acttcagcac
    88261 gagaaacttt gttttcctta tattcactta ctgtatcgac aactttattg aagctcttgt
    88321 ctgaaatcga tccattatct ccaccattgt aatcagaacc agaattacca atcaacagtg
    88381 gacgaagtcc tgcccagctt gcttcgatat cattcaatgt gatatttgct tctggatagc
    88441 gatgattgat cacatccaat agataatcta catcttcttg tgtgactttc ggatccgtaa
    88501 aatctccttg gtaatccgta tctgtcgtac caaagtaagt cttgttttct cttggaatag
    88561 caaaaaccat ccgcccatct tgctttcctg tatcgaagta tgtgggttgc ggtacaggca
    88621 gttttttcgc atctacaact aaatggatcc ctttagttgg acgcatttta ggagagactg
    88681 gacgcgtaaa attcaagttt cgtactttat ctacccaagg accactcgta ttgatcacta
    88741 attttgcttt gatttcgatc acttcatctg tcagcaggtc acgagctttg acgccaacga
    88801 tttgatcgcc atcatataaa aagccaaccg ctttcatttt acttactaga taagccccat
    88861 cttcggcagc ctttttgata ttatcaatca ctaaacgggc atcattgttg cggaaatcta
    88921 gatagacacc cgcgcctttt agcccttctt ttttcaaaaa cggttctctt tccaatactt
    88981 cttctggtgt cagcgtataa ttctcatatt tggttcctgt cacatttgcc agtttgtcat
    89041 aaaggtccat tgctactttg accgagaaca tattgaaggt tgttgctcct tcgccctcat
    89101 agattggcaa aagcattgga tctggttttg ggatgtgtgg ggcaatactt tgtacgactg
    89161 cacgttcacc aactgtgtca gctactactt ccacatcaaa tgtcttcaga taacgaatac
    89221 cgccatgaac aagtttggtc gagcgagagg aagtcccttc tgcaaaatcc tgcatttcga
    89281 tcaatcctgt tttgattcct gatgctgctg actggactgc cacaccggca ccagtgattc
    89341 cgccaccgat gatcaacaga tccaattctt cttgctgcat tttttgaatg ctatcttgtc
    89401 ttgtcttgtt tgaaaacata tggatttcct ccttattcaa atgcttgggt agcttttact
    89461 gcttttttcc aaccgttgta aagtttttct cggcgctctt cctccatttg aacttcaaat
    89521 agcttgccag cttcataaaa ttcgcggatc tcgtctgtat ctttccaata accgacagct
    89581 aatcctgcta aaaatgctgc ccctagcgcc gttgtttcca agttttctgc tcgttggatc
    89641 ggtacattca agatatctgt ttggaattgc atcagatatt cattgtttgc tgcaccgcca
    89701 tctactttca atacaggaat cttgattcca gtgtcatctt gcatcgtatc gatgatatct
    89761 ctcacttgat aagcaatcga ctgtaaagta gctttaatga tatcttcttt tgtcgtacca
    89821 cgtgtcaagc caaacatagc ccctcgtgct gcctgatccc aataaggagc acctagccca
    89881 acgaatgcag gaacaacata aacctcatct tcgcttgtag agcgtttcgc tgcgtcttct
    89941 gaatcgctag ctttttgcaa catttgaagt ccatctctca gccattgaat cgaagaacca
    90001 gccacaaaaa tacttccttc taaagcatag taaacctttc catttatgcc gtaaccaatt
    90061 gtcgtcaata aattattttt ggataattgc ggtttttcac cggtattcat gacaataaat
    90121 gaacctgtac cataagtatt cttgaccatg cctggttcga atgccatttg tccgaataat
    90181 gcggcttgct ggtctcctgc catccctgaa attggtgctt cgcttccata aaaatgataa
    90241 ccttgcgtat aaccataaac ttctgagtta gaagtgactt ttggtaacat cactcgagga
    90301 atatttagta aatctaaaat ctcctgatcc caatctagct gatggatatt gaacaacatc
    90361 gtccgactgg cattcgagta atcagtaaca tgtgcttgtc catcggtcag tttccatact
    90421 aaccaagaat cgatcgttcc aaacatcaat tcgccttttt cagctcgttc ttgcgcgcct
    90481 tctacatgat caagaatcca gcgtatcttc gttgctgaga aataggcatc gatcactaac
    90541 cctgtttttt cgtggaaaaa ttcttgatag ccttcatttt ttaagttatc tgcaattcca
    90601 gctgactgtc tggattgcca aacaactgca tgatagattg gacgtcctgt atctttttcc
    90661 catattactg ttgtttcacg ctggttcgtt atgccaatcc cagcaatctg atttggtttg
    90721 atccctgatt cgatgaacgc tccggcaata actgactgta cagaattcca gatttcattt
    90781 gcatcatgtt ccacccaact ttcttgcggg aaatattgcg tgaactcttt ttgagaactg
    90841 ccgatgtgtc ttcctttctt gtcaaagata attgctcgtg agctagttgt accttggtca
    90901 atcgccatga tatacgttga ttctgtcatt gtgattccct ccattatatg caaacgcttt
    90961 ctttaactac tcttatcata ctactgtttt aaaaaaagaa actgtcaaaa ttttattctt
    91021 tttttctttt tttgacagtt ttttattatg aagctatttt ttaatcaatt cttttctttt
    91081 ttcagcgtct ctattcttct tttcaatcga ataatgtcgt aaggtgacgc aaattctgac
    91141 agataatatt cacactgttt tttgtttctc tggcaaaatg tgatcaccaa atcatattct
    91201 ttaccttctt cataacttcc aatctcaata ccgccgattc ccctcagttc tctgatcaga
    91261 tattgttgaa aagcaatacg ataaatcggc agactttcta aatcgattcc aatcactact
    91321 ggttttgcaa tataaaaatc gatcatcaaa agaacattgg catactggat catcaggtaa
    91381 ctaagctcac tatcttcagc tcttttcctc ggcaattgct gcaccgctgt ttcctgtaat
    91441 ttcgccagta actgtgttaa ggaatgcccg agcattttct gctgctgttc caaaagccga
    91501 tcacgctcat agacttcgat ctttccaaca aagaaaaacc attcattgtt gatctgggaa
    91561 atttggtagc ctactgcctt ttcttctttg attttcaaac gacgaggacg ataatgccat
    91621 aacatcgttt cccgaatgta agtatctaaa acagcggttg gtaacttttt gctccgtgtc
    91681 aaatcatact gataaaatat ttcttcgtct actatcgaaa aagaaacaaa aaagctgtaa
    91741 aacatcattg tttcgtatac attcagttct gctgctgttc gactaagatg catcacgatg
    91801 attggatcga ttgcttgata aagccggtcc gactgataga gtaatttctt ttctttcaaa
    91861 gttgcgtacg tacttttttc attcaatagt ctttttttcg taatcccgag ccagcaagca
    91921 atctgtgctt ctgctgatgc cgtaaacgtt gtttctaata cacggtccaa tcctagaatg
    91981 aacggtcttt tttctggtgt attttgcaaa aaaagcggtc gctgatcttc cgggatatat
    92041 tggaatagtt cataataaaa atatcgtatc tgtagctctt ctccgtataa ttgaccgttt
    92101 ttgatttgta atccaaattc ctctaagagc tggttcaatt ctttgatctt tcgaaaaaca
    92161 gaagactcac tgatattaaa cgctgtcgct aatcgaacga tggatacttg ctgatgttct
    92221 aataaataga cgagcatctt aaacttcaag ctttcctgca ataaaaacaa tagaattttt
    92281 tccaaactta catcttcgtc caacacaagc agcgcctgat actcatttcg ctgtacttct
    92341 actttttttt ccatcattcg gccaatcaat tcaatatctt ccaaatattg atcaaacgag
    92401 gcttttgaca gttggacatg ctctctcaaa ccattgatag aggctgttcc cccttgcaga
    92461 acaaggtttc gcaaaatcag gatctgtaac tgctcttttc tttctaataa tgcttcaatc
    92521 ttcataggca gtattcgcca cctttgacca caccatatct ggcacacggt attctggctg
    92581 gaaaaatagc tcatgttctt ctaactgttc aaacgtccat ccttcttcta ctgcaattgt
    92641 cagttggtca agaaggtgga acatctcttt tttgctttgg agctgtgctc caagaatctt
    92701 tttttcagaa tcaaatagaa atttgagttt aactttgctt tgatttattg ctgtaagctt
    92761 gtagtctacg tatcctgttt caattttttg gggataaaaa atgctctctt cttctgtcaa
    92821 acctacgctt cctaagaaat agccaaacca ctgattgccg actggacgat aggttccctg
    92881 atcctttgtc tgtgagccac taattgtctt gctgacattt cgtgctgtac ggacagcgtt
    92941 atttactaga gaaacataca ttttttcatt tgttggacga aaagttactt ggatcgcatc
    93001 gccaatcgca taaacgtctt tttgagacgt ttgcaagtag tcatctaccc agatcgttcc
    93061 atcatcattc aatgttaatt gttcttccca catctgattg tcgggacgag aatgattagc
    93121 cagcaaaaca agatcatagt tctcctttct tttttccgtc tctacaatag cggtttcatt
    93181 ttcggtcaaa gacacaactt gttcttctaa aaaaagtgaa agcccttgat ttttcatttc
    93241 ctttactaac ggttctgtca tttcaggatc tagataacga aaaagaattg actttcggct
    93301 ttcgtaaaga tggatttgtt tttttcttga tgccaaccct tcagctattt cgattcctgc
    93361 ttgtcctgca ccaataattg caattttctt tgcattcatg atcttttgct gaagagccgt
    93421 tacgttttgt atctttttaa ctgggtgaat aagcgttgat tctgcagaaa tatttcgaaa
    93481 cttttgattt gaaccagtcg ctagaatcaa acgatcaaaa tccaattttt ttcctcctgc
    93541 taaaacgact tttttttctt tcaatcctat tactgtcttt tctgtgtaaa catggattcg
    93601 atagcggtca gtcagttctt ctttcgtgat ccaagaaagt tgtttttcac tggtaacctt
    93661 tccttttaat atcaaattga acccgctggg aatccatcct aacttcgatt gcttttcaat
    93721 caaatatatt tcacttgttg gcatcaattg gcgaagataa atagccgcat gaatccctgc
    93781 aaacgacccg ccgacaatta caatgttcat ctgcattcct ctttcttata aggtgttcta
    93841 ctgctaactt ctcaatcaga aaacacagta ttcataactc gctttcaacc ttatgataca
    93901 tagcgatgca gcagaatcaa ccctctccat cataccgtgt tctaggtctg cttctcaata
    93961 atgtactagt ggacctaatt gaagagtatc atttctgaag agatgtttca aaaaatgtac
    94021 tcttataaaa gaaaaaaact cgccttttac gtacctctgt ccaaatagga aacagaaaag
    94081 aacgaaaaga cgagttttag cgtgtgtaat gtaatagtta gaatagacac atcatttatt
    94141 tttaattctt taatgcttca acaactgctt cgcctaattg atgagcagat tgaggatttt
    94201 gccctgtaag aagccgccca tcaatttgaa ccacttctga aaaagctgct ccagactgat
    94261 agtttgctcc tttttctttc aaagcatctt ctgctaagaa tggtacttca ttcgttgtac
    94321 catttgcttc ctcttcttca ttactgaacc cagtcactgt acgtccagct aaaatagatt
    94381 ttccagactc atctgtaact ggcaacaatc ctacaacacc gtgacaaaca gctgagacca
    94441 gcccttcatt tcgatagatt tgtgaagcga ctttcccaag atctattgct tctggaaaat
    94501 cccacattgt tccgtggcca ccagcataat aaaccgctgc ataatcattt gcatccacat
    94561 catcaggacg taaggtattt cccaaggccc gatttctgaa atcttcatct tcataatagg
    94621 cccaatcgat ttcatccata ttcaaaacac tttgcggttc taaaggtaca tatccgcctt
    94681 taggactgac aaaatctgca tcgatcccat tttttactaa tacatcataa aaatgagtca
    94741 gttcacttaa ccacaaacct gtaggacgct cgatcttttc gtacttttct acatttgtta
    94801 atacgatcaa tactttcatt ttcccatcct ctttctataa aaattgcgtt attgataacg
    94861 tgctttcaag aaattatata atgggcttgg caaataaagc tctttatatg ctttttcaaa
    94921 aggtttcgtt acaaattcat cctcacggtt ttccaataat ttctctgccc agtcaggatc
    94981 gacgatcatt tgctgtccaa ttgctgcaag ttctgcatat ttcaatacct gctctacatc
    95041 ttcgcgattg cggatcccac cgacaccaac taaagggatc tttccttgaa tcgtgttatg
    95101 gaccacttca agaatcgatt tatcgttgta tttatcagaa cgagctattc gatcatacat
    95161 atttaatgaa acatgcagat aatctagttt tgactcacgt aatcgttcaa gaagccagat
    95221 cgtgtcttca aaacggatac ctggtgtttc atattcttct ggtgagaaac gataccctac
    95281 gatgaatggt tttttagcat attgttcaac cgctgagaaa accgttgtta ataattcttc
    95341 aataaaacga taacgatttt ccaagctacc gccccattga tcctctcgtc tattcgaatg
    95401 cggagagaaa aattgttgga tcaaatacgt atttgctcca tgcaactcga ttccatcaaa
    95461 tcctgattca atcgctctac gtgtggcttt gccaaatgct tggattgttt caacgatttc
    95521 ttcttcactc attgctcgtg gtgtttccgc atcaggacgt tttgctgcaa ctgcacttgc
    95581 agatacaggc tgttctccag ataaagttgc tcgacttgtc attcgtccac cgtggaaaat
    95641 ctgaataatc gcttttgcgc cttctccttg gattgctttc gctagctcac ttaatcgagg
    95701 aatcatttca tcatgagcga tgctgagttc acctggccag ccttttccga tatcttggac
    95761 atttgcagca ccagtaataa cagcaccaag acctttagaa cgttgtttat aataagtgat
    95821 ctcatcttcg gtgatcactc cattgaaaaa gctgagttgt gtcgtcatag gcgacagcat
    95881 caatcggttc ttcaattgaa ctcctgaagg taaagtaatt gattgatcaa attgtgacat
    95941 tgtcattcct cccataattc gctctgatta acctatcgag aatcatctat cattaaacca
    96001 ctcgattagt tgtacatgca actaatttgc ttaaaaaatt ttattttttt tgtaacttat
    96061 ctgttgcatc attcattgtc tgggctaatt ctttacttgc ttcatttccc aaaactgctt
    96121 tgtatgactg atgaagttct tcccagctac catcgacttc cttagcaaat tggcgtcctt
    96181 gttctgtaag ataaactaat gacaagcgac cttcgctaac tgttttgacg tatcctttat
    96241 attctaattt ttcaacaaaa cgagcaatcg ttgatggggc ggaatagagc atttgtgcaa
    96301 tatctttttg tggtattcca tctttttctt ttaatatcaa taaaataaaa ccataagttg
    96361 gtgccagacc tgtccttcta aacgcttcat cagctaattt attcaacatg cgatctagct
    96421 ttttcacgct aaagaaaaga cattcatcta acgctttttc aattggcttt tccatttcat
    96481 cccctccatt ggcgtacata tcatagtatg atttttattt tttgtcaaaa aacacggaat
    96541 aatgtccgcc tcctctgtct agatttgaaa aaaagaagta gccatcttca aagaatttcg
    96601 gcttgttttc gacaaaactc cttctggaac accgtgaatc agaggttgtg aaaaagtcgt
    96661 ttagttttga gtatgaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
    96721 tggcttaata ccgaaaaact ggcttttgaa caccgtttat atcagaggtt gtgaaaaagc
    96781 cgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat tttgtcgttt
    96841 tttgagctaa tgccgatggt cagctgattg tacaccgttt attgagaggt cgtgagcctg
    96901 ccgttgaact ccgagtcaca aggagcaatt tcaaaaaaca gcttctcatg ttttatgact
    96961 atggtgactt gtgatcgagg agttggcagg agaatactgt ttattcgtaa agaaggggct
    97021 gtgacaagac ttttgtcaca gtcccttatt tccttttatt ataggatttc gccatttgat
    97081 tggatgactt gtctgtacca atcaaatgat tttttcttta accgtttctt tgtgccattc
    97141 ccttcgtcat cttgatcaac atagatatat ccataacgtt ttgacatttg tgaagtagaa
    97201 gcgctgacga tatcaatgca gccccacgac gtataaccaa ataaatctac tccatctttg
    97261 atagcttcct tcatctgctc aatatgctgc ttcaaatagt caatgcggta atcgtcttga
    97321 acggttccat cttcttctac tttgtctata gctccgagtc cgttttcagc tatgaacagt
    97381 ggtttttgat acctatcata taattgattc aaagcaattc tcaaaccctt aggatcaatc
    97441 tgccagcccc attcacttga tggaagatac ggatttttaa ttcctccagt tattaagttt
    97501 ccttggaccc cttcccaatt ttctggatga gcactgactg cactagacat gtagtagcta
    97561 aaagaaacaa aatcaacagg gtaggctgct aagatttctt catcacctac ttctattttt
    97621 aagttgacac ctttttcttc aaacattcgt tgcgtatacg ctggataata tcctcttgct
    97681 tgaacatcac taaagaacaa agaagttctt tgctgctcaa ttgttttttg aatatcatcc
    97741 ggatcacaag aatacggaac aagaagctga tagctgatca tacaaccgac ttgtgaacct
    97801 ttgataattt catgacaagc ttttgtagct aaagcacttg ctataaactg atggtgtgca
    97861 ccttggtatt tcgcttgttc caaatgggga tggttttctt caattactcc tacgctaaca
    97921 aaagggtgat gttttacaca gttgatctca ttgaaagtca tccaatattt tactttattt
    97981 ttatagcgtt tgaatactgt ttcagcgaaa cgaacgtaaa actgaataag ctttcgatta
    98041 ggccatccac catattctgt aaccaaatat aacggcattt cataatggga aagagaaacg
    98101 actggctcaa tcccatattt tagacattcg tcaaacactt gatcataaaa aaataatccc
    98161 gcttcattgg gtgtttcttc atcaccatga ggaaaaattc ttgtccatgc gatagacatc
    98221 cgatagcatt taaaacccat ttcagcaaat aaacggatgt cttctttaaa gtgatgatag
    98281 aaatcatttc cgtgtctttt aggataataa tacttgctgt tgggatcctg cgccgctttt
    98341 acttgctgat gcgtcatcat atgccattga tctgtccatt tttctttagg taaatcgctg
    98401 tcgaatgtat acacatcaga cacagataat cctttttcat ctacatccca tgcaccttca
    98461 cattgattgg ccgctgtcgc tccgccccat aaaaatcctt ctggaaattt tttcatatcg
    98521 atccccctaa acttgctagt tttcttgttt gattattttt cttatgacta gtaatgatat
    98581 ttttgaatct actactattt gtaacaataa ccattgtggt cggatcatat ccctctttga
    98641 ttaattcttg caaatctact tccatcaata aatcgccttt acgtactttt tttccatcag
    98701 atactgcact ttgaaagtat ttgccattta aattcactgt atcaataccg atatggatca
    98761 gtatctctgc tccttcttca ctgactaccc caattgcatg tttcgttgga aaaactgttg
    98821 aaacttcccc gtttacagaa ctgtagattt ttccgtccgt tggaatgatc gcaaaccctt
    98881 ctcctaaaga tccactagca aatgttggat cgtttacttc agataaatca attatttcac
    98941 ctgctgcagg agtacttaac actatatcct ttgaggattc ttcttcgatt tcatccttgt
    99001 agccgaaaag aaaagttagc atacaggaac tgatcatcgc taagaaataa ctagcaacta
    99061 gccaaacaaa tccttcacca atataaatcg ggaaagacaa aatacttctt gtagcaactg
    99121 ccaaagcacc ggctcctgcg gcaccagcta ctccaccaaa tatagctcca catatacaag
    99181 caatgtaaaa tggtcgttta tatttaagag tcacaccata aacagcaggt tcagtgattc
    99241 caaataatgc tgtgataaat gcagataaag ataaactttt tactttctta tttttcgttt
    99301 tcaaaaatac cccaagtact gctcctgctt gcgcagctac ggcagtcata catgctggac
    99361 ctagcgtatc acgaccgtag aaagaaagat tgttggtcat tactggtaca agtccccaat
    99421 gtaacccaaa aatcactaac acttgccaaa atccgccgat aacagcacca gataaaacag
    99481 ggctaaagtt ataaacagct aaatatccag ctgcaatcag ctgacttgca taatctgcaa
    99541 ttggacctac aactaataaa ataattggga ccatcacgat gatacaaatg aatggtgtca
    99601 ataaatttcg aatactttcg tgcaagcttt tgcgaatctt aggctcaata taagaaagaa
    99661 cccatacagc taaaattgct ggaataaccg tacttgtata ccttgctaga atcacgggca
    99721 tccctagaaa tcgcaaggtt atgctatcac tatatgcact gacaatcgta ggataaacga
    99781 gagctgctgc aataaccata gcgatgaaac gatctgtatt gaactttttt gcagcagtat
    99841 atgccaaaaa tataggcaag aaatagaaaa atgcatcagc tgctgcattc aaaatgatat
    99901 aagttcctga ttcagtagtt aataaattca aactcgtaca taaggcgagt actcctttga
    99961 tcaaaccact tcctgccaag acgcctaaag tcggggcaaa aacagcggcg atcatgtcta
   100021 taaaagagtt taaaatgcct tttttctcag ttcctctctt tccactagat gcatcatctt
   100081 ctagtttcat ttgtttggat aatacgtcat acacttcatt tactgcattt cctactacga
   100141 cttgaaacat tcctccagct tcgactacag tgatcacacc ttctagttgc tctaccttct
   100201 ccttatctgc cttcttatta tcttttaggg taaagcgtaa ccttgttgca caatggtaaa
   100261 cattgctgac attttcttcg cctccgacaa atcggacaat atctttcgct aattgtgaat
   100321 agtccatttt tacttccccc ttgttaatta atgccgtagc aatccttctt acaagataag
   100381 tatagttctt agaaaacggt ttctccattc aattagtacc ccttctaata gcgaacgaaa
   100441 ctgtacaata gtgtgtatgg tttacttagc cgagcaaatc actcactgct tgaaagaaaa
   100501 caagtatatc gtttctctta tagtcaaagg agacaaaaaa aacacgcttg atcttccttt
   100561 gctgatgtat aagtggtatc tcaagctgat actctgtgat ttttgtttct acttggcttt
   100621 tgataagaca gcacaacaaa gtctcggcat ttaacggaag cataactagg tcagaactag
   100681 gagagattag ttgctgaatg gaatctatct ttttatggat ttttatttga ttttcttctt
   100741 tgtagtaatg atcaagcagt tttgttttct ttttttcttc aactatggtc agcttttcac
   100801 gcaaaatctg ataaatctcc caagattcaa actcatctag aacactaggt agataaatca
   100861 aatcatccca ctcaaaaata agctcttgct cagtagtcaa aattgcatca tattccgtta
   100921 ttttgtttga ttgatttttg aaaagataca ctggttctat ccggcattct ttgatccaca
   100981 tgttcatact ttgttgaatc tgttttttta gtgtattcaa aatactaatt ggctggttac
   101041 taacaatcag tatctcccct tgttttcgaa aaggattcaa cgcttcggca agatacaaaa
   101101 caaaataagc gctttcagct aaattgggac gtaattgaaa atgtattggg aagaaacgcc
   101161 gaattaaata aatttctaaa ggataacggg agatgatctt ttgtacaaaa tgattcatgg
   101221 tattatgtcc ggcatccatc ctcgttttca tttgtttgat atgggttagc aaaaattctg
   101281 tttcatggaa caataacgaa acaggaagat ctaaaaaacg aataatcgtt tgctcaaatg
   101341 ctcttagatt cttctctatt tctttgtttt tttcatcatt aatcatcaaa taatttaact
   101401 ctaatagtcg attttcaatt gcttgttttt cttgattgga aaaagaatag ggtagttctg
   101461 atgataactt ttttgtgagg gtctctacca tcggaataaa taatgtatac ctgtttgttt
   101521 tttctaaaag ttcttctctt cgaagaatgg tcaaaacgag ataccgagaa aatcgtatga
   101581 agtcctctcc tgaaacaata taattatatg atataagaac ctcttttaat gtcttttgga
   101641 cagtcggcat gatattggaa aaaattgtac tgatttgttg aggaaacctt gcgtgttgga
   101701 tcaccgtttc tgttccaaca agagataaga aaattctttt tgaattttct tcagcaatga
   101761 tttttagtcc ttttgtcgac gaaacttcca gttctaattt aggattgcgc tcgatccaac
   101821 gcaaaatcgt ttttatttgt aatgatacgg ctgtttttga aagaaagaaa atgtctgcta
   101881 tcttttgcat actgacatac cctttttgaa acaataagta cgtcacaata gttgaagctc
   101941 ggaaatccat gctggaatag attttatgtt tcgtcacctc ttgtatgatt tgctgtttag
   102001 tttcagattc aattgattct aaaaaataac ctgaacgttt atttgactgg ataatgattt
   102061 tcttctcgta gatagcaagc atatttttga tttcagtttg gatcgttttc ttgttggtac
   102121 ctaaagcttc agctaattct tcgcttgtta tccaatgatt cttatttctt aatactacaa
   102181 gaatttggag ttgtcgttta tttaagtcca tttgcttaca cctcctcttt gttataaaca
   102241 tacaggaaaa acctctcaaa tgacaatagt cacttgcaga ggtttaaaaa tcatttgaag
   102301 aaagatctat attcaaggtt tttaccatta gactggataa tttgtttata atattcaaaa
   102361 ctatcttttt tcaatctcct aaagcttccg ctcccatcat tttgtctatc cacataaata
   102421 aagccatatc gcttcctcat ttcccctgtt cctgccgata cgatatcgat tggtccccac
   102481 cataagtaac caatgatttg gcaaccatct tttattgcct cagcaatttc ctgaagatgt
   102541 atttttagat actcacaacg ttttggatcg tggattcgat tcaattcatc tggtgcttca
   102601 tctagtccaa tgccattttc aactatgaat aatggtaatt cataacggtc tgtcaattca
   102661 ttcatgacat aacgaaaccc tagaggatca attggccaac tccaaggttc gggagatttt
   102721 tccttcaaaa agggattatc gaaaccattt gctccgccag tatctacgcg catttctgct
   102781 tcttttttat agacagcgct acgataataa ctaaatgcaa tataatctgc cgtgttagct
   102841 tgcaagcact gttcgtctcc atctaacatt actggtgcac agtcttcttc cttccatgtc
   102901 ctgtaaatat aagctggata atgccctcgg cacatgacat cggagaaaaa ccatgaattg
   102961 cgtttaaatt gcagtgaacc aaatacattc tctggatcac agtcatatgc ataagtcgct
   103021 aatgcagaaa agctacacat aatacccatc ttagcatttg gatcgatttt ctttaataat
   103081 tgagtagcac gggcgttcgc tagaaacata tgatgacagg cttgataaat cacttttttt
   103141 gttagtgtag caatgggttc aagtttatct ctagcaggga atgtaaccaa tccagcagct
   103201 gcataaggca tacgaaaagc attattgatc tcattaaagg tcatccaata tttcactttc
   103261 ccttggtagc gttcaaagac ggtctgcgca taaaattcaa aaaagtcgat taattttcga
   103321 ttttgccacc ccccatactc tcctaccaat gctaaggggg tttcataatg actaagagta
   103381 attactggct cgatatcata tcgatttaat gtattgatta gtctgtcata aaatacgaga
   103441 ccagcctcat ttggcttctg ttcgtctcct cttggaaata ttcgtgccca tgagatgctt
   103501 gttcgaaaag cttttaaacc taactccttc agtaaacgaa tatcctcttc aaatcgatga
   103561 taaaaatcaa tcgcttcatg agaaagataa tggagtgatt cgtctaaagc aatctcccaa
   103621 atcttttttt cttcattcca ctgaatacta ggatgctggt tcatgatccc gataagcgta
   103681 tctgtcacat taggtaattt gccgtcttcc aaccatgcac cttcacattg attagcagct
   103741 actgctccgc cccataaaaa attttctgga aatatttcct gatccataac tatctctcct
   103801 cgctatcaac aatttcttac ttgaagtata gctcgattca tcaaaacaag atattctaac
   103861 tatttcggca gaagagaaga aaaaaattga atgctactct tcatttttaa tatttatata
   103921 taacaaatac ataagacgat ccccgaaaga aaacactcgt taaccatcta agcaaacgat
   103981 ttattttact tccaacacaa atgcttcata aggtcgtaaa tcaactttgc cgtctggtaa
   104041 ctgtttcaga ggataatttg acacaataac ccttaaagct tcacgtggaa tagtgaacgt
   104101 ctgcttttca ctggacatat tacaaataac gagccattgc tggtcttcat atatgcgaga
   104161 ataggcaaag acatgctcag gcgtcttttc cactaattca tagtctcccc agacgatgat
   104221 gtcatgtttt ctacgcaaat cgatcaattt cttgtacata taaaaaacag aatcttgatc
   104281 tgctaacgct tcttctgcat tgatttcttt ataatttgga ttgactcgaa gccatggaat
   104341 tccagtagta aacccagcat gttcacgggc gttccattgc ataggcgtcc tagcattgtc
   104401 tcttcctttc gccttgattg attccaagat ttcctctttt gtaaatccag aagaaatcct
   104461 ttcgttatac atattgattg tctcaatatc ttttgcttcc gcgatatcat cgattgggca
   104521 attcgtcatg cctaactctt ctccttgata gatataaggg gtgcctttca tcatatggag
   104581 caagatagca aacatcttgg cactttcttt tcgatacaca tcgtcatttc cccaacgaga
   104641 aacaattcgc ggaagatcgt gattattcca aaataaacta ttccatcctt catcacctaa
   104701 agatgtttgc catttggata atactttttt cagttcagag atactcaagg gtttcaaatc
   104761 ccatttgtct ttgccttctt gctgatctaa tcctatgtgt tcgaattgga agaccatgga
   104821 taattcattt cttttaggat tggaataaag tttagcgatt tcggtagttg ctccccatgt
   104881 ttcgccaaca gtcatcagat ttttgtcacc aaaagttgct tgattcattt cctgaagata
   104941 ctcatgcagt ttaggtccat tacttgtgat ttctttatcc ggttctttgc caatcatatc
   105001 gatcacgtcc atacgaaagc ccccaatacc tttatcgatc cagaaattca tcatttgata
   105061 aatctcttgg cgaacctgtt tatttttcca attcaaatca ggttgacgct tgctatacaa
   105121 gtgcagaaaa tattgccccg ttttttcatc caactgccaa gcagaaccac tgaatattga
   105181 gcgcaatcta ttaggctctt ttccattgac cggatcacgc caaatataat agtcccgata
   105241 aggattttct ttatttttac gggcctcaat gaaccacgga tgttcatcag aagtatgatt
   105301 caccaccaga tccatgatga tacggatatt tcttttcttt gcttcttcaa tcagttcttc
   105361 catttcttcc atcgtaccga attctgccat gattgcttga taattactga tatcataccc
   105421 attgtcatca ttcggagact cgtatactgg agaaagccaa attgcatcaa tacctaaata
   105481 cgccagataa tccaacctgg aaataatccc ccgcaaatct ccaataccat cttgatttga
   105541 atcttgaaaa cttcttggat agatttgata aacaactgaa tggtgccacc atttttgttc
   105601 catgattttc ctcctataaa tggatgtttc tattttttgc ttaatcattt attttagtct
   105661 ctctcactaa aaattgtaac gctttcaaat agctattgca ccttgttatc ggtaacagaa
   105721 taaaacaaaa aaacagctga ggaaattccc tcaactgttt tttgaaagga ttgatttatg
   105781 cagggaagaa tgctgatttc acaacttctt tataaacggt gttcactcag ctgtcccttg
   105841 gtattagctc aaaaaacggc aaaatataag aaactatttt ttcgtttttt tctctaatac
   105901 tcaggccaga acgctgattt cacaacctct ttatttcaca ttcaatatcc acatgaatcc
   105961 cacaaataca aagaacagac catacatgat tggagatact tcttttcctc ttttagctgc
   106021 taacatggta attggataga agataaaacc taatgcaatc ccatctgaaa tactataagt
   106081 caaaggcatg ccgatcaaga tcaagaatga agggatcgca atctctaagt ttccccattc
   106141 gatttgtcgc aaagattgtg ccatcaatac tcctaccata atcaatgctg gtgcagttac
   106201 ttgtgaagta acaactgata gtaatggaga aaagaacaag cctaacaaga agaaaatccc
   106261 tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg ataccagctg atgattctac
   106321 aaatgcacca acaggagaag taccaaataa ggaacccgca agcatagctg ttgaatcaga
   106381 agctaaagct ttccctacac gaggcatctt gttgtctttc atgaaacctg cttggtttgc
   106441 taatccaact aaagttcctg ctgtatcaaa gaaagtcaca agcaagaatg tcagcactac
   106501 gacccacatt tgaagagaat tgatgtcccc tacatgtttt aatgcaacta aaaatgttgg
   106561 tttcaaactt ggtgctgcgg caacaatttc agaaggcatt ggaatcaaac ctgtgaccag
   106621 acctaaaatc gttgtagctg ccatcccgat gaagatgcca cctggtacac gccgaacaag
   106681 caagatagca gttacgacta atccaaaaat cgtcaaccaa gtagaaccaa cgttgagtga
   106741 tcctaatcct actaatgtag attcattcga aacgatgatc ccaccttcgc tcagaccaag
   106801 gaaagcgata aatagaccga taccgccaga aatcgcatat ttcaaatctg ctgggatcgc
   106861 atcaatgatc atttcccgca gcttgaagat tgtgatcaaa ataaaaatca aagaagcaac
   106921 gaatacgccg gctaatgctg tttcccatgg aatccccatt cctaaacaaa cagaataagc
   106981 aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt ggataacggg ctaatacccc
   107041 cattaaaaca cagccgatcg cactggctaa tgcagtcgca gtaaacactg ctccttcatc
   107101 cattccagaa gcacctaata cagttggatt tacaaataag atataagcca ttgagataaa
   107161 tgttgtaaat cctgccagta cctcacgttt taccgtggtg ttcaattttt ctatttcgaa
   107221 gtaggaaagt attttttcct tcatcttcca tccccctaaa tccgaacatt ataaataaag
   107281 tccgttaaaa tttcgattta ttaataaaac aacgtgtttt atcatatact aaaacaaaac
   107341 ataagtaaac aaaaaattcg gattttaagc aaaatccgaa cgttccatct gattaaatcc
   107401 ctttatctca aagttcatga acattcactt tggattacaa gaatttcttt attgtgttaa
   107461 taaagtttct tcattctagt ctcaaaaatc aatagtgtga tttgatttta ccatttcgtt
   107521 tctgttcttc tttgatgaat tcttttgtat cgacaggatt cagattattt agtccgccaa
   107581 aacctgtatc gaccattttt ccccagcctt ttgtcttctt ttttcctgat ttgagattct
   107641 ttgctcgaag cttctcacta tgttttgctc gccgatactt caaataacct tctaatcttc
   107701 ccattagatc cactgttttt cctccttttt attcaatcag acgactcagc ttttcctata
   107761 acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca agatgccatt gttatctgtg
   107821 atttctcctg gttcttccat gttctcgtag atagtttcaa attcatccgt aaaaaatact
   107881 aaagaaggcg gtggtcctaa tacctctggt gaaaacttct gcacaaatac ttttgggaat
   107941 acgcctaatt ctaattcttt tgagatgctg agtacaatac tcttaaattc ttctggcaat
   108001 tcgattgttt cagaaatttc tgcaccaatt ttttctacaa agaatcggca aatcatctct
   108061 acgtcattga cataaagcat cacacgtgtt ttttgtatca tcatttgacc cttctttcta
   108121 tttgattttc tttcctgtct ataaactgat tccattatat cacgaaagcg caaacacctt
   108181 gtgtttgata ctattcaaga aaaataaagc aatagtttct aggattgctt tcctccacaa
   108241 acttttttta caaaaaaaaa acggcaatga atgccgctct tcttatttcg attcttttat
   108301 aattctgctt ctgattcatc tttggttagt gtgtgtctaa cactagttac gccaaatgtt
   108361 tgatcgattt tttccttcaa cgcttcgatt gcctgtgcgc cacgtgccgc ttgttcagca
   108421 ttgatggatt caatgaccag caccgctgct tgtgtttctt ccgtcagcat cgttctttgg
   108481 gcttcacgcc agttgagaca gcgacagatc gctccttgtt catcatagta gatgatttct
   108541 tccggaagtg ccggagcatc ggaatctgca cctaaaggca ggaatgattc gccaccttgg
   108601 gcttttccta aatgcagatt tcctgcgatc ttttcgatat cttctccgcc gcaaggtacg
   108661 ccaaaagaca aggaaatact attatagata tctacaagcg gattgattgg gaaaaattcc
   108721 cgttcttgat ttgcacgttt cagtaaagct tcaatagaag aacgtgcacc ttttttagtc
   108781 ttgaatgtgg taaatgcctg acgccactga gcaatgactt cgttttggct gaatgtttct
   108841 tctgtaaggt aatttcttgc ttcttttgct gcctcattca ataattgatg atacgtttct
   108901 tcactttctt ttgttacgtg gttatcgata ccatttacgg ataagatttc gatggttgct
   108961 tcaggaaata cttcccagaa attggtatcc actattactt ttttcatctg ttcacacctc
   109021 ttgattagaa taattgcttg atgaatagat agtctgtttg gggatcatct cccatataaa
   109081 aaacatgttc gctgtatttg ataaatcctt ttttacgata aaaagcgagc gcaacatcgt
   109141 tgtgttccca aacaccgagc caaatggctg ttttttgctt ttcttttgct ttttcaatag
   109201 ctgtatctag taaaaatgta ccgatacctt gatgtttaaa tttttttcgt acatagattc
   109261 gctcgatttc taaaaaatga tcagccattt cctcagattg actcgttcct gtatttagtt
   109321 tcaggtaacc cgcaggctct tgatcgatac tcactaggaa aaaatcggtt tctttatctt
   109381 ttagctcttc tgtaagtttt tctagtgcat aagcttgtgt caagtaatca gctaaatgct
   109441 ccggtgaatt atcttttcca aatgtatctg taaatgtttc gatactaatt tgttgtagta
   109501 agtctacatc tgaaggcgtc atttttcgaa tttcgtattt attcacggct tcttcctttc
   109561 aatagatcct tttattgcct tttttgacaa aattccaatc ttcgctaaca ttcttgcata
   109621 ctttatttaa aagatattcc aattgttttg cttcttcgtc agaaagacca tttaatgcga
   109681 ctgcattgga gtagttgttt tctcgaatga tgaaaggata gatttctgct ccttttttag
   109741 tcggatagag tttcttgatt tttttatttt ctttatcttc caaacgttcg atcatcccat
   109801 tggattctaa tttcttgatt gctcgggcag tcgttgtacg atctactttg atcatttctg
   109861 ctagtttttc ttggatgatt ccaggatttt cgcaaatacg tactaaataa agatactgtc
   109921 ctcttgttaa atcgacttct ttgaattcga tattgctaat agaatctagc gctcgggcaa
   109981 tcattccgat gtctcgaagg atttcagcca tttcatccca cctttatttt gtttgtaaga
   110041 ctagttcacc tctttttgtt gcaaatgcaa ctattttcaa taaaaaaatc aagttctttt
   110101 ttaataacta gtaaagtata ttcttttctt cttttacgaa aaagctcaat cacaatagat
   110161 ctggtttttg ttttctaaaa taagtaactg tttcttcgtt gttcgctttt ctgatagctt
   110221 tggcaactct tctatactaa aaaatcccat gttggaagtt tcattattct caataaactt
   110281 ggcctcttca ccgtggatcg cacaagcaaa aacaagttta taatactgaa aaagctgtgg
   110341 aatatctttt tgtttattcg tatcaaaaac tgctcgcaac tgagtagttt ctactgttaa
   110401 tcctgtttct tcatagactt ctttacgcac attttcttca ggagacaatc ctatttcagc
   110461 aaatccacca ggaagtgacc attctttcgt ccgtttatct tcgattagta ggaccttgcc
   110521 atcttttttg atataggcac gaacatctac ttttggtgtt ggatgtcctt ctccaatcgt
   110581 tgtgagcgct tttatttctt cgaaagtttc ttttcctatt tcactgatca gttccaatga
   110641 aatcgtccgg atctcctcaa aacgctcttt atcaaataca tcttttccat aatatagacc
   110701 agcttgagaa agtgcgagta gtcttttgta ttgagccaac ttcttgttca ttctcttccc
   110761 ttctttttta gcaaatgtat gactatgtaa tcaagtatag aagactggtt taattacggc
   110821 aaataaatat aatgaaatct gcaataaata aaagaatgtt aaaaaactcg agaacaaaag
   110881 acattcgact tctctggggc tgtgaataaa tggcgttcaa aagcttgcat ctgcggtaat
   110941 aagccccacc aaacgaaaaa tatgacgagc tattcttgtc tggctgttct tattaattga
   111001 ggcaggacag ctttttcaca acctccccac taaacggtgt tctcctacca actcctcggt
   111061 cacaagtcac aatattcatc aaacatgaga agctgttttt ttgaaattgc tccttgtgac
   111121 tcggagctga acgacaggct cacgacctct cggtaaacag cgttcaaaag ccaatttttc
   111181 gatattaagc catttctttg ttacttattg tcattgattt ctttttctaa tttatccaat
   111241 agtggagtgt aatccacttg agtatagtct gttacatcaa attcatatag tgttcctaac
   111301 tggaatttgt catattttcg gtcggcgatg atctttttga atgcttcttt cgtaatatga
   111361 ttggtccctt tcgttcgatc ttccaatacg tcaccatagt gataatgatc catgatatag
   111421 ctcaaatgac gatctgattc acgatcacgt ttgtaccgac gttgccataa cacttcaaag
   111481 tctgctgtta ggcgaatcgt gatcacctga tatccgtatc tatctgctaa ttcttggagt
   111541 cgtgcttttt gcttatcact aaaaggatac tcggacacaa tcactttctt tcctgcttcc
   111601 atatacaaat caagtgcttt gtaatagaaa ggataaactt tcgtcctttc ttgttccgcc
   111661 cgttcttcca aattgttaaa cccgatggat tctgcatagt attccttgat ttcatcgagc
   111721 gtgatggcat acatatctgg aaatcgttcc cgtagtttat tcataagata tgtttttcct
   111781 gtaccaggac taccagccag aagaatgaaa tactttttca tctcatttat ttcctaagtt
   111841 aattggactt tccaagttca taaacagtcc aatcttatgc ttgacaattt cttttactgc
   111901 ttcatttgct ggaatcacat tatggcgaag agacttgttg atttctgttt cactaaaggt
   111961 ttctttagct actttcgtcc agttgactaa aagctccgtt ccaacattga atttgcggat
   112021 cccgttattg atcaattcag gataatcttc ttctttcaca cctgttcctc catggataac
   112081 tagggggaca tcaactactg catcgatttc tttcagcaag ggaatgttta cttctgtttt
   112141 tgatttgaat tgtccatgat tcgtaccgat agcaatagct aatgcatcca cgcctgtagc
   112201 ttctacaaat ttcactgcgt cttccggtct tgtatatacc ttgtcatttt cagcgacatg
   112261 gattccttct tctgtaccgc cgattgttcc aagttctcct tctacggaaa caccgtgcgc
   112321 atgggcatat tctactactg cttttgtctt caggatattt tctttgaaag gcagatggga
   112381 cccatcatac atgacagaag tatatccgct gtcgattgct tctttgatat catcaaagtc
   112441 tcgggcgtgg tctaagtgca agactgcatc aaccatttct tcttctgcaa tagctgtcat
   112501 tacattgact aacacatgca tgccgatata tttagcggta tcgacacttg tttgaataat
   112561 gatcggcgaa cccatttctt ttgctgcgcc tagcatagca ggaagcattt ctaggttatg
   112621 cgtattaaaa gctccaactg taaaattcaa ctcttctgct gtttttgtta cttctttcaa
   112681 tgttgtatac atcttttatt gccaccttat ctttttattt gtttcctttg atcgttactt
   112741 gttttgcaat ttgtcgcatc tcatccattt tgcccatgta gtaatcgatc ccttgatcat
   112801 caccagcacg agcagcatct gcacgcttct ggttatagag gctcatcagt tttttatacc
   112861 catctccgat cgtctgttta ttttccaaac tcttttctaa atattggatg gcttgatcgt
   112921 tcacatacaa ttcagaaaat ttaagtccaa cttgttcata aagtttcgct aatttatgct
   112981 tatcttgggt agtggaaatc tcttgtttca attgtgtaat ctctgattga atcttttcgg
   113041 tcgcttctgg cgatagggct ttttcttctt ttgccagaac ttttttcggt tctttttctt
   113101 ttttcttaaa aaatccaaac atcattttcc ctactttcaa ttaaaatgct ttcaaaactg
   113161 ggcttaagat ccaggcatat aagagtgctg cggctactgt atctacatcg gttgctgtag
   113221 cattgttgaa tcccatcgaa ttaaagatcg ttactagcaa agcgggcaat aatgtaataa
   113281 agaaaccatg agcaataccg ccaatgatcg ctcctcggcg tccgccaact gcgttgccaa
   113341 atatccctgc tgtccctccg gcaaagaaat tggttagcat accaggtaaa atcatcgcta
   113401 agccgaacat gggcaaagta aacatggcaa taatcgtacc aatcgttgtc gtaatgaaac
   113461 ctaaaatgac agcatttggg ctataaggaa agaaaactgg acaatctaag gcagggatcg
   113521 catcaggaac taatttcata gctatccctc gaaatgccgg cacgatttca cctaacagta
   113581 gacgtacacc agctagcaat acatacacac ccactacgaa ttggatggct tgtaaaaagg
   113641 caaacatgat atagttttga tctcctgata attcagaagc gaatggttcg cctgcaaata
   113701 aaacagtgat gatatatagc gggaccatca cgaccatgac agacaaataa gtatcttgca
   113761 gaaattcgaa atgtgtcggc aacttgatat cttcgatcga ctttttattt tcgcctttct
   113821 caccaaaaag cttagcaaca ccggcttcaa ataagtaccc gatcgtacaa aaatgaccta
   113881 aagcaatatc atttgatcca gtgattttac gaatgatcgg ttgcgctact gccggcatag
   113941 caatggcgaa acaagctccg ataaaaccac cgaccaagat caatacgatc cctcgcagac
   114001 cggcaaaata gccaaagact gtcgtcatcg ttgccatcca taaaattgct tgaccagtta
   114061 aaaaaatgta cttccatttt gtgaaacgag ccaaaataat attgaataca aaaatggcta
   114121 agaacgtcag tgcaatatct ctacctaaac ccaaatcatt cattgcttga ccattgatag
   114181 attcaataga agggatgatc ccttgcatat gaaatccttc agtaaagatt ttcccgaaat
   114241 aagttaaact tccaacgatg atgctcgaac cggcactcaa cacttgaaat cctaggagtg
   114301 tctttaacgt gccagagatg acttgtcctg ctgatttctt ctgcagtagt agacccaaca
   114361 tagcaatcaa tgcaatggtg atcgatgcct gagtcaaaat attttcgatg ataaaattga
   114421 taacgcccat g
//
LOCUS       DAZKOB010000002        87429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000002 DAZKOB010000000
VERSION     DAZKOB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.002"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1208
                     /locus_tag="ACG8M6_000100"
     CDS             207..1208
                     /locus_tag="ACG8M6_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0455804.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            1227..2246
                     /locus_tag="ACG8M6_000101"
     CDS             1227..2246
                     /locus_tag="ACG8M6_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0455805.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2348..3064)
                     /locus_tag="ACG8M6_000102"
     CDS             complement(2348..3064)
                     /locus_tag="ACG8M6_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0455806.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3033..3818)
                     /locus_tag="ACG8M6_000103"
     CDS             complement(3033..3818)
                     /locus_tag="ACG8M6_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0455807.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3809..4558)
                     /locus_tag="ACG8M6_000104"
     CDS             complement(3809..4558)
                     /locus_tag="ACG8M6_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0455808.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4856..5380
                     /locus_tag="ACG8M6_000105"
     CDS             4856..5380
                     /locus_tag="ACG8M6_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0455809.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5396..6895
                     /locus_tag="ACG8M6_000106"
     CDS             5396..6895
                     /locus_tag="ACG8M6_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0455810.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6983..7585)
                     /locus_tag="ACG8M6_000107"
     CDS             complement(6983..7585)
                     /locus_tag="ACG8M6_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0455811.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7689..8645
                     /locus_tag="ACG8M6_000108"
     CDS             7689..8645
                     /locus_tag="ACG8M6_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0455812.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8950..9906)
                     /locus_tag="ACG8M6_000109"
     CDS             complement(8950..9906)
                     /locus_tag="ACG8M6_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0455813.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10080..10640
                     /locus_tag="ACG8M6_000110"
     CDS             10080..10640
                     /locus_tag="ACG8M6_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455814.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10633..11457
                     /locus_tag="ACG8M6_000111"
     CDS             10633..11457
                     /locus_tag="ACG8M6_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0455815.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11467..12084
                     /locus_tag="ACG8M6_000112"
     CDS             11467..12084
                     /locus_tag="ACG8M6_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0455816.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12102..12785
                     /locus_tag="ACG8M6_000113"
     CDS             12102..12785
                     /locus_tag="ACG8M6_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0455817.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13468..14751
                     /locus_tag="ACG8M6_000114"
     CDS             13468..14751
                     /locus_tag="ACG8M6_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0455818.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14767..15654
                     /locus_tag="ACG8M6_000115"
     CDS             14767..15654
                     /locus_tag="ACG8M6_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0455819.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15671..16507
                     /locus_tag="ACG8M6_000116"
     CDS             15671..16507
                     /locus_tag="ACG8M6_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0455820.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16510..17133
                     /locus_tag="ACG8M6_000117"
     CDS             16510..17133
                     /locus_tag="ACG8M6_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455821.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17130..18422
                     /locus_tag="ACG8M6_000118"
     CDS             17130..18422
                     /locus_tag="ACG8M6_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0455822.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18781..18878
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18994..19575
                     /locus_tag="ACG8M6_000119"
     CDS             18994..19575
                     /locus_tag="ACG8M6_000119"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0455823.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19578..20885
                     /locus_tag="ACG8M6_000120"
     CDS             19578..20885
                     /locus_tag="ACG8M6_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0455824.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21301..21789
                     /gene="purE"
                     /locus_tag="ACG8M6_000121"
     CDS             21301..21789
                     /gene="purE"
                     /locus_tag="ACG8M6_000121"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0455825.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21782..22906
                     /gene="purK"
                     /locus_tag="ACG8M6_000122"
     CDS             21782..22906
                     /gene="purK"
                     /locus_tag="ACG8M6_000122"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0455826.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22919..24214
                     /gene="purB"
                     /locus_tag="ACG8M6_000123"
     CDS             22919..24214
                     /gene="purB"
                     /locus_tag="ACG8M6_000123"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0455827.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24336..25724)
                     /gene="ltrA"
                     /locus_tag="ACG8M6_000124"
     CDS             complement(24336..25724)
                     /gene="ltrA"
                     /locus_tag="ACG8M6_000124"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0455828.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(26293..26670)
                     /locus_tag="ACG8M6_000125"
     CDS             complement(26293..26670)
                     /locus_tag="ACG8M6_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455829.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            26926..27654
                     /gene="purC"
                     /locus_tag="ACG8M6_000126"
     CDS             26926..27654
                     /gene="purC"
                     /locus_tag="ACG8M6_000126"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0455830.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            27654..27908
                     /gene="purS"
                     /locus_tag="ACG8M6_000127"
     CDS             27654..27908
                     /gene="purS"
                     /locus_tag="ACG8M6_000127"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0455831.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            27910..28581
                     /gene="purQ"
                     /locus_tag="ACG8M6_000128"
     CDS             27910..28581
                     /gene="purQ"
                     /locus_tag="ACG8M6_000128"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0455832.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            28582..30804
                     /gene="purL"
                     /locus_tag="ACG8M6_000129"
     CDS             28582..30804
                     /gene="purL"
                     /locus_tag="ACG8M6_000129"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0455833.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            30789..32228
                     /gene="purF"
                     /locus_tag="ACG8M6_000130"
     CDS             30789..32228
                     /gene="purF"
                     /locus_tag="ACG8M6_000130"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0455834.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32251..33303
                     /gene="purM"
                     /locus_tag="ACG8M6_000131"
     CDS             32251..33303
                     /gene="purM"
                     /locus_tag="ACG8M6_000131"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0455835.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            33300..33878
                     /gene="purN"
                     /locus_tag="ACG8M6_000132"
     CDS             33300..33878
                     /gene="purN"
                     /locus_tag="ACG8M6_000132"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0455836.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            33875..35410
                     /gene="purH"
                     /locus_tag="ACG8M6_000133"
     CDS             33875..35410
                     /gene="purH"
                     /locus_tag="ACG8M6_000133"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0455837.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35426..36700
                     /gene="purD"
                     /locus_tag="ACG8M6_000134"
     CDS             35426..36700
                     /gene="purD"
                     /locus_tag="ACG8M6_000134"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0455838.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            36822..37736
                     /locus_tag="ACG8M6_000135"
     CDS             36822..37736
                     /locus_tag="ACG8M6_000135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0455839.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(37869..39434)
                     /locus_tag="ACG8M6_000136"
     CDS             complement(37869..39434)
                     /locus_tag="ACG8M6_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0455840.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39427..40878)
                     /locus_tag="ACG8M6_000137"
     CDS             complement(39427..40878)
                     /locus_tag="ACG8M6_000137"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0455841.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(40892..41908)
                     /locus_tag="ACG8M6_000138"
     CDS             complement(40892..41908)
                     /locus_tag="ACG8M6_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0455842.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            42128..43216
                     /gene="chvE"
                     /locus_tag="ACG8M6_000139"
     CDS             42128..43216
                     /gene="chvE"
                     /locus_tag="ACG8M6_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0455843.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43270..44796
                     /gene="mmsA"
                     /locus_tag="ACG8M6_000140"
     CDS             43270..44796
                     /gene="mmsA"
                     /locus_tag="ACG8M6_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0455844.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            44798..46003
                     /gene="mmsB"
                     /locus_tag="ACG8M6_000141"
     CDS             44798..46003
                     /gene="mmsB"
                     /locus_tag="ACG8M6_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0455845.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            46260..47237
                     /gene="guaC"
                     /locus_tag="ACG8M6_000142"
     CDS             46260..47237
                     /gene="guaC"
                     /locus_tag="ACG8M6_000142"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0455846.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            47501..48925
                     /locus_tag="ACG8M6_000143"
     CDS             47501..48925
                     /locus_tag="ACG8M6_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0455847.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(48967..49224)
                     /locus_tag="ACG8M6_000144"
     CDS             complement(48967..49224)
                     /locus_tag="ACG8M6_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455848.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            49386..50495
                     /gene="pdhA"
                     /locus_tag="ACG8M6_000145"
     CDS             49386..50495
                     /gene="pdhA"
                     /locus_tag="ACG8M6_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0455849.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            50498..51475
                     /locus_tag="ACG8M6_000146"
     CDS             50498..51475
                     /locus_tag="ACG8M6_000146"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0455850.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            51500..53143
                     /locus_tag="ACG8M6_000147"
     CDS             51500..53143
                     /locus_tag="ACG8M6_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0455851.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            53151..54557
                     /gene="lpdA"
                     /locus_tag="ACG8M6_000148"
     CDS             53151..54557
                     /gene="lpdA"
                     /locus_tag="ACG8M6_000148"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0455852.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            54719..54994
                     /locus_tag="ACG8M6_000149"
     CDS             54719..54994
                     /locus_tag="ACG8M6_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0455853.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            55000..55767
                     /locus_tag="ACG8M6_000150"
     CDS             55000..55767
                     /locus_tag="ACG8M6_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0455854.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            55935..57764
                     /gene="typA"
                     /locus_tag="ACG8M6_000151"
     CDS             55935..57764
                     /gene="typA"
                     /locus_tag="ACG8M6_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0455855.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            58025..59797
                     /gene="recQ"
                     /locus_tag="ACG8M6_000152"
     CDS             58025..59797
                     /gene="recQ"
                     /locus_tag="ACG8M6_000152"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0455856.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            59911..60213
                     /locus_tag="ACG8M6_000153"
     CDS             59911..60213
                     /locus_tag="ACG8M6_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0455857.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            60216..61379
                     /locus_tag="ACG8M6_000154"
     CDS             60216..61379
                     /locus_tag="ACG8M6_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0455858.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            61405..64833
                     /locus_tag="ACG8M6_000155"
     CDS             61405..64833
                     /locus_tag="ACG8M6_000155"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0455859.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            64854..65978
                     /locus_tag="ACG8M6_000156"
     CDS             64854..65978
                     /locus_tag="ACG8M6_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0455860.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            65959..66405
                     /locus_tag="ACG8M6_000157"
     CDS             65959..66405
                     /locus_tag="ACG8M6_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0455861.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            66389..66727
                     /locus_tag="ACG8M6_000158"
     CDS             66389..66727
                     /locus_tag="ACG8M6_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0455862.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            66846..67406
                     /gene="rsmD"
                     /locus_tag="ACG8M6_000159"
     CDS             66846..67406
                     /gene="rsmD"
                     /locus_tag="ACG8M6_000159"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0455863.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            67403..67894
                     /gene="coaD"
                     /locus_tag="ACG8M6_000160"
     CDS             67403..67894
                     /gene="coaD"
                     /locus_tag="ACG8M6_000160"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0455864.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            67884..68927
                     /locus_tag="ACG8M6_000161"
     CDS             67884..68927
                     /locus_tag="ACG8M6_000161"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0455865.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            69086..69814
                     /locus_tag="ACG8M6_000162"
     CDS             69086..69814
                     /locus_tag="ACG8M6_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0455866.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            69930..70175
                     /locus_tag="ACG8M6_000163"
     CDS             69930..70175
                     /locus_tag="ACG8M6_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0455867.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            70172..70390
                     /locus_tag="ACG8M6_000164"
     CDS             70172..70390
                     /locus_tag="ACG8M6_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0455868.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            70526..71065
                     /locus_tag="ACG8M6_000165"
     CDS             70526..71065
                     /locus_tag="ACG8M6_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0455869.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            71058..73331
                     /locus_tag="ACG8M6_000166"
     CDS             71058..73331
                     /locus_tag="ACG8M6_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0455870.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            73369..74415
                     /gene="holA"
                     /locus_tag="ACG8M6_000167"
     CDS             73369..74415
                     /gene="holA"
                     /locus_tag="ACG8M6_000167"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0455871.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(74504..74758)
                     /gene="rpsT"
                     /locus_tag="ACG8M6_000168"
     CDS             complement(74504..74758)
                     /gene="rpsT"
                     /locus_tag="ACG8M6_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0455872.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(75158..75427)
                     /locus_tag="ACG8M6_000169"
     CDS             complement(75158..75427)
                     /locus_tag="ACG8M6_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455873.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(75689..75817)
                     /locus_tag="ACG8M6_000170"
     CDS             complement(75689..75817)
                     /locus_tag="ACG8M6_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455874.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(75897..76352)
                     /locus_tag="ACG8M6_000171"
     CDS             complement(75897..76352)
                     /locus_tag="ACG8M6_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0455875.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(76490..78418)
                     /locus_tag="ACG8M6_000172"
     CDS             complement(76490..78418)
                     /locus_tag="ACG8M6_000172"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0455876.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(78615..79448)
                     /locus_tag="ACG8M6_000173"
     CDS             complement(78615..79448)
                     /locus_tag="ACG8M6_000173"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0455877.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(79640..80344)
                     /locus_tag="ACG8M6_000174"
     CDS             complement(79640..80344)
                     /locus_tag="ACG8M6_000174"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0455878.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(80367..80993)
                     /locus_tag="ACG8M6_000175"
     CDS             complement(80367..80993)
                     /locus_tag="ACG8M6_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0455879.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            81319..83694
                     /locus_tag="ACG8M6_000176"
     CDS             81319..83694
                     /locus_tag="ACG8M6_000176"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0455880.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(83718..84209)
                     /locus_tag="ACG8M6_000177"
     CDS             complement(83718..84209)
                     /locus_tag="ACG8M6_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0455881.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            84396..85316
                     /locus_tag="ACG8M6_000178"
     CDS             84396..85316
                     /locus_tag="ACG8M6_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0455882.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            85470..85814
                     /locus_tag="ACG8M6_000179"
     CDS             85470..85814
                     /locus_tag="ACG8M6_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0455883.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(86004..86504)
                     /locus_tag="ACG8M6_000180"
     CDS             complement(86004..86504)
                     /locus_tag="ACG8M6_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0455884.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            87032..87115
                     /locus_tag="ACG8M6_000181"
     tRNA            87032..87115
                     /locus_tag="ACG8M6_000181"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87066..87068,aa:Leu,seq:cag)
BASE COUNT        28202 a        15251 c        18685 g        25291 t
ORIGIN      
        1 aatcacttta cataagtttc aaaagtttct ttaatttcct attgttcaat aagtaacaat
       61 gtaaagataa aattaaaaat gttaatacgt attagcaaaa taaaagcttt ttttattgtg
      121 aataaatgaa cataattagg aggagaaaga tgaacttatt tgacattttt cgatcgactt
      181 tgacagatat gtcgattgtc agtgcaatca cttcaacagt ttttattata ttattagggt
      241 ttgtttgtag aaaaaagggg attttttctg aatctgttgg aaaaattcta tcgaaagtcg
      301 tcttaacagt ggctttgcca gcactggcat ttcgagcttt catgcaagat atcaatacag
      361 aattactgat gcaaggaatc aatgtcttga tttggggatt agttatttat attattctta
      421 ttctagtttc aaaacctttt ttcttaagct ataaagggga taagcaaaca acgttgcgtg
      481 tcctgacgat ctttggttct acgacctttt tcggaacgcc tatcgtcagt gcgatttatg
      541 gtcctgtcgg cgtgatgtat tcctcaattt tcaatattgg ctaccgaatc tttttgtatt
      601 cttatggata cataaaaatg agtggcttga agatggaagc taaaaatatc aaagcgatgt
      661 tcgtcaatcc aatcattatc gctacttttg ccggcctttt catttggctg ttccaagggt
      721 atctgccaca agtgtcagtt acaggtgcag aaggagagat aagccaagta gcgtttttgc
      781 gaattgacca gactgcaccg tggctatttc agtcgatgga ttatttagct ggtttaagtt
      841 caccattggc gtggctagca atcggcgcaa cattaggtga agtaagcttc aaaaaagctg
      901 catctgaaaa aacttcatgg tattacagtc tagtcaaagt ggtatttgta cctgcattga
      961 atatcgtgat tctagcagtt ttgacggcaa cgaatattct accagtcaat tttgaagcac
     1021 tggcaacaat cgttatcatg atggctacac cagctgcaac agtggcagca gcctatgcta
     1081 tcagttttga tagggaggca cttttagcct ccaatgcatc cttactgtca actatttttg
     1141 ctgtggtact gacacctgta tggatcgttg tattacaaat gattcatggc ataggtttat
     1201 tttcataaga aatggaggaa gaaagtatga gaatcgcatg ttacggtgtt cgtccgaacg
     1261 agatagattt ttttgaaaaa ttgaatattt accattatga tctcagtctt tatgaagaac
     1321 tgttgacaca tgacaatatc gtgtcagcaa aggaccatga tgctgtattg ttgaggggaa
     1381 attgtatagc agatgagacc aatcttgcaa aaatgcagga atatgggatt cgttatgttt
     1441 ttacacggac tgtgggagtc aatcatatcg atctacaagc ggcagctgat tttggtatga
     1501 cagtagcccg tgtaccatcg tattcgccaa atgcgatagc agagttatct ttgacttttg
     1561 ccatgatgct tttgagaaat actgcgtata cgacgatccg aacatctttc aaagattttc
     1621 gagtggatga gcaaatgttt agtcgtgaaa tcagaaattg cacagtaggg atcataggaa
     1681 caggcaggat agggttgacg gaagctgttt tgtttaaggg actaggagcc aaagtgatcg
     1741 gctacgatat tcatcagaca gaagctgcta aaaaagtatt gacgttccac tcgcttgaag
     1801 agttattagc caaaagcgat attgtcagta ttcatgtacc tcatatccca ggtgaaaacg
     1861 atcaaatgat caatgaagga tttttagctc aaatgaaaaa aggtagcatc ttgattaata
     1921 cagcccgtgg agagcttcaa gataatgaag ctattttaag agctttgaaa agcaatcatt
     1981 tagctggttt tgcaaccgat gtttttgcaa atgaaacaga ggtttttttc cgatccttca
     2041 agccatggga gacgattcca gaccctgcta tccagcaatt agtagaaatg taccctagag
     2101 ttcttgtaac cccgcatgta ggttcaaata cagatgaggc attatcaaat atgatttcga
     2161 caagtttcga gaactttcgt gaaataatag aaactggaaa aacgaaaaat gaggtatctc
     2221 ttcctaaagc ccgtcaatta agataagaaa acatggatat tcgtcaataa aatgtttaaa
     2281 aatagtccaa gtaaacagtt gttacttctg tatacttgga ctattttgta tgggataatt
     2341 cgatgactca acagtaatct gtgagctttt tccgcccatt ctttgtcaat tcataacagg
     2401 ttagcggccg gccgatttcc aaatatttga ttctttcgtt caatatatct tgttctacta
     2461 aaaaattcaa atatttcttg acggatatac gtgacagtga gcttttttga gccagttctt
     2521 ttgttgaaaa aatagtatct tcttttagaa taaatgcact tatttttttt aaagtcagtt
     2581 ttgacagacc tttaggcaaa tcgatgaatg atggtatttc gtgaatggga gtggtgtggt
     2641 tgaatacttg atcgatcaat ttctgatcgg ctttttcagt cgtttggatt gtctgagaga
     2701 aagcaagata cttttcgatc gccagctgaa aacgttgcaa agtgaacggt ttgatcaaat
     2761 aatctaaggc accgtagcgg atcacattct ttatggtttc gatatcatct gcagcagtga
     2821 tcaaaatcac tgggatatca tttccttgct gtctaagcca agataaaaaa tggataccac
     2881 tttcgccagg catgtataca tcaagcagca gcagatctgc gtttttttgt ctgaaatatt
     2941 tttttccttc ttctacattt ctgaaacttt cgattacgtt catattaggg atgctttcca
     3001 caaaagtacg attcaattct gcaaccattg gatcatcttc gattatcagg acatttgtca
     3061 tcattttgat cttccttttt tggtaattct agatagacga ttgtaccgcc atttgtttgt
     3121 ttggaaaatt ctaaaaatcc accgtggcgt ttgacgattg aatggatgag attcaatcca
     3181 aacccacgat tgattccctt cgtcgaaaaa cctagcgtaa atattttttc tgagatttct
     3241 tctgatatcc cgacaccgtc atcgcagacg gtcacgatga atacttcagc ttctgtgtca
     3301 tagtttaaat gaatccggac ctgtccgttt ctttcatgtt tttgtagcac ggcttctttt
     3361 gcattatcaa gtaagttccc taacacttgc aaaagtgaat ggatggagtc atccatctcc
     3421 attttaggga taaaagaatc gctttctaac agcatttcaa cagattgttc tttggcttcg
     3481 tttattttgc ctaataaaaa accggcaatt gcaggtactt tgatcagact tgagacttgg
     3541 ccaatctctt ttttataatt ttgatttagc tgctggataa aatgggtgac ttcttcatac
     3601 ttcttcaaat caatcaaacc agaaatgaca tgaagtttat tcatgaattc atgggtttga
     3661 gctctcatgg agtttatata ttgtttcgtc cccccaagct cctccattaa tttcattact
     3721 tctgatcgat ttcgtaaagt tgcgactgct cctaaaaaac ggtcattgct aaaaagaggt
     3781 gtaacgctag aaatagtttc tatcccattt aaatacatgg attgagtccg aacggtcgtt
     3841 tgatgctcaa tcgtctcttt aaacaaaacg tcatatagat tatcactgat ttttttccta
     3901 cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt gtattgatca
     3961 agaggatagt tttatcagaa gagatggcta agattccttc atctacttca ttttcgataa
     4021 tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc agtacttttt
     4081 tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca ataatcaatg
     4141 aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg tttgttaagc
     4201 caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa tagcggaagc
     4261 cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc acttttttca
     4321 catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga gaatatcgaa
     4381 tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg atttcatttg
     4441 ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg atgagtttat
     4501 cattggcaac gattttagca atgctagaga ttttatctga cacactagat cggttcatcg
     4561 aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat gtggagaaac
     4621 aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa aaaggaaaca
     4681 taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa atttaacaaa
     4741 ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta aaagaagcta
     4801 tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg tgtatatgat
     4861 agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg caggattggc
     4921 agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg atccgatgct
     4981 tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc gtttaacaga
     5041 tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt tggtattttt
     5101 gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt cgtcttctga
     5161 aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac gaggtatggg
     5221 agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt tttacagccc
     5281 ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca aggaaagaat
     5341 ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt gttaaatgga
     5401 aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga tgttgctgat
     5461 cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca tcagtggaag
     5521 ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca aaattgtgcg
     5581 tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca gtctattatc
     5641 gagtaaattg gacgtctcca cccctgaagt gtatacggaa caaggcgtac cggtgatgtg
     5701 tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta cagcagcaga
     5761 acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag tattagaagg
     5821 acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa atcgcgataa
     5881 gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg ggttgatcat
     5941 cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact ctttagggaa
     6001 accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag ctctaaaaga
     6061 aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat tacagcgaca
     6121 aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct acaaacaaga
     6181 acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg cccgagcaca
     6241 acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgtcaaa aacaaatcga
     6301 actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag tgaagaaaaa
     6361 agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag tcaaagaagt
     6421 gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg aagccaataa
     6481 gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa aagcagaagc
     6541 cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag ctgtcttgag
     6601 tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc taggaaatat
     6661 cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg gagcgaaccg
     6721 agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa aagcgacaac
     6781 aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta ctagccaagt
     6841 aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag aataaagtag
     6901 tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc tctccatttt
     6961 tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt tggatcttag
     7021 cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt tcgaccaaat
     7081 cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa ttgataccga
     7141 ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac aaagcggatt
     7201 cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa atgataccgg
     7261 aattattgtt aataccatct gacacgacga tcacaataat aactagaata ggaagcaaga
     7321 tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa atgatagcta
     7381 atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg gttgtagtca
     7441 agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt cctcgttgag
     7501 tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc gtacgcatac
     7561 ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt actatcatac
     7621 ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa taaaaaaggg
     7681 ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc ttcttttcga
     7741 tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc attgcattat
     7801 taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga atcgcagcct
     7861 ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat actatttttg
     7921 gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa ttaaccgaag
     7981 ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac atgtcttggc
     8041 agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct ttgcttgcaa
     8101 atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag atcgaaaaag
     8161 aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat aaagaagatg
     8221 tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa gatgccagag
     8281 aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac ttctctatga
     8341 gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga atccagcaac
     8401 cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa acattttcag
     8461 agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta ttgtatcggc
     8521 aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg ttccagtttt
     8581 tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa aaaatccaaa
     8641 aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac aacctcttct
     8701 ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa caaacatgag
     8761 aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct cacaacctct
     8821 ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa acgaaaaata
     8881 ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac
     8941 cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt cgatgacatc
     9001 ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta ccatattctt
     9061 tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct ttgtaccatc
     9121 tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca cataaccgtt
     9181 ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc tgacagcagc
     9241 aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc ctgtttcaaa
     9301 aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac gagcgaaaga
     9361 tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat tgaagaaccg
     9421 gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt ttaaagcccc
     9481 cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt tgaagattgt
     9541 catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt cagccagttc
     9601 ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt ccaatgctgc
     9661 caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt cgatttcagc
     9721 atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac ggtgagcgaa
     9781 gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg tctcttgatt
     9841 gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc attgatattt
     9901 tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct attattataa
     9961 caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat gaaagcagcg
    10021 atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag gggataaacg
    10081 tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt gaaactgaaa
    10141 aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa tggaaagatg
    10201 gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc actgattatg
    10261 aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa aaacgaaacg
    10321 ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg cttagcagca
    10381 aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt agataccttg
    10441 acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg aattttcgtc
    10501 tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc gattgggtga
    10561 atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa gaacaacgaa
    10621 aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat gatcgcagca
    10681 atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa tcaagcagtc
    10741 gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac aaccagcagc
    10801 aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga tgttaaaaca
    10861 gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga aattgatgag
    10921 tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat tgctccagaa
    10981 tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat ggtctcagct
    11041 taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga tgtgatgagt
    11101 cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat tacggagcca
    11161 ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa ctggtacaac
    11221 agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc taaatggatc
    11281 gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca cgaagatata
    11341 actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa cgctgagtat
    11401 atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga aaagtaggaa
    11461 ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg ttggccagca
    11521 gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa gaatctgtct
    11581 tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca atttattgaa
    11641 cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc tagtatcatt
    11701 ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag caaatacaat
    11761 aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga aacaaaggaa
    11821 tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga ttcatgggaa
    11881 cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa tcctgcgctg
    11941 tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt gcaagatgca
    12001 ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga gacgtatcaa
    12061 ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa caatttacgc
    12121 cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc attttagaag
    12181 caagcgggat caagatattt ggaaaattga tccgttcgat catctttact acggatattg
    12241 cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt acacctcatc
    12301 cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc ggtgtaggtg
    12361 gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa gcgcaaggtt
    12421 cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa gtttgtcgag
    12481 tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata gatgaaaagt
    12541 tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca gaagcagtac
    12601 gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt ccaacggaag
    12661 aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg ccaccaagta
    12721 gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa aagaatcatg
    12781 aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc tcgtcttttg
    12841 tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt ttttttacct
    12901 gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa caaaaaaatg
    12961 tcttgattta ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta aaaaaggcac
    13021 ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga actgaccaat
    13081 caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat ttcatacaga
    13141 attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca tgcttattta
    13201 gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt aattatcctc
    13261 ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact atctaatttt
    13321 tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct tggtttttat
    13381 tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt atattgaaaa
    13441 ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat cttagcttta
    13501 agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa aatcacgctg
    13561 aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt aatcgagctt
    13621 tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta tgaaggatat
    13681 tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt ccaactgaac
    13741 aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt ggagcaaggg
    13801 ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta cgacgggcaa
    13861 ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa aaatatattt
    13921 gaacaattaa atatccctga accagcttca aattggacat gggatgaatt tgaacaaatc
    13981 agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga agattttata
    14041 gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta taatcgggaa
    14101 gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt tttccagctg
    14161 cgtaaaaatc tggtagacaa aggggcccat ccagaaccag gtggtggagc tgcaagtacg
    14221 acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt tctatcaagt
    14281 aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca gttgattaat
    14341 ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc gcaaatgctg
    14401 agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga tttctttcaa
    14461 aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat catgtcgaat
    14521 attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt tgattattta
    14581 gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc tttgaatccg
    14641 gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga agtaacagca
    14701 gaagaagcag cagagagtct ttatgagttt gcgtcaaaca ttctaggata gcagcaggag
    14761 gaaggcatga aaaaagaaaa actaaaacgg ttcttggatc aagacaatgt ggtcggttac
    14821 gtatttgctt ctccattcat cctaggattt ctattgataa cagtgatacc gatgatcttg
    14881 tcactttatt attcgttcac cgattatcgt ttgggaaatc cagtctcatg gatcggcttg
    14941 gataattata taaagatgct gagtgatgcc cgttttttga actccatccg agtcactttt
    15001 ctctatgtat ttgcatctgt acctgtgaaa ctgatctttg gtctattgat tgcttatctt
    15061 ttaacgagga atatcaaagg aacgactttc tttcgttctt tgtattatgt gcctagtttg
    15121 atcggtggca gtatttctat cgctctagtt tggcgggagt tgttttctaa aaacgggtta
    15181 gtgaatattt tcctggaacg attaggcgca cctgcgcttt cctggtttag cgatcaaaaa
    15241 ctggtcatga tccctcttgt tttgatgtct gcttggcaat tcggttcggc aatgatcatc
    15301 tttgctgcag gtatcaaaga gatcccaggc tcgtattatg aagcggcaga gatcgatgga
    15361 gcgaacaaat ggcagacgtt ttacaagatc acgttacctc ttctttcgcc gatcattttg
    15421 tataacttgg ttatgcagac aatcgcggcg tttatgactt ttacgcaggc atttatcatt
    15481 acccaaggtg gtccgaatga tgccacgaac ttttatgctc tttatgtgta taaccaagct
    15541 ttttctttta attctatggg gtatgctagt gctatttcgt ggatcatgct gttgattatg
    15601 ggagctgtca cctttctgat tttcaaatca tcaaaaaaat gggtcgttgt gtgaatgaga
    15661 ggaggaagaa atgaataata cagtaacgaa atggtttcaa tacggtttta ttttgctatt
    15721 aggtctagtc atgatttatc cggtcttatg gatgatcgca ggttctttta aaacggatca
    15781 agagattatg agtggttcgc tgaatttgat tccagagact tttcaatggg aaaattacgc
    15841 aaaaggatgg gcggggtttg ctggaatttc attttttact ttctttagga attcgtttgt
    15901 catctctgca gtttcgacga tagggacagt tctgagctcg acttgtattg cttacgcatt
    15961 atcgagaatc gatttcaaag gaaaaagatt ctggttcgtc gtcatgttag taacgatgat
    16021 gattcctgca caagtgattt tgattccgca gtttattatt tacaatcggc tgaatttggt
    16081 tgggacgtat gtgccgctga ttttaccaca tttttttgga caagcatttt tcatttacca
    16141 gatcatgcaa ttcatggtca gcatccccaa agagttagat gaatcagcga ttattgacgg
    16201 atgcagcaag tatagtgtct ttaccaaaat tatttttcct ttgctgaaac cttcgatcat
    16261 cacgacgatc atcatccagt tttattggaa atgggacgac ttcatgggac cattgattta
    16321 tttaaacaag ccgagatctt acacggtatc gattgccatc aaattatttg cagacgctgg
    16381 atctagtacg acttacgcga gtatgtttgc gatgtctacg ttgtcgctgc taccggtttt
    16441 tctgattttc ttattcttca ataaatatct tgtccagggg atcagtacaa gcggtctgaa
    16501 gggctgaata tgaaaaagcc aatagaagag atgtttcttt acaagctcaa ctactatgtc
    16561 tgtcggattt ttttaaccag tcttatcagt aatgctcttt tcgctttagt tctaggatgt
    16621 ttgttcactt taggattagt gctgtctgct cgtgttgtct atctgattta ttttttgatg
    16681 atgtatcctt cttattatct atctgtccat tacttggcag aaaaaaaggt atgttggagg
    16741 aaaggaaaaa tcaaacggat gtgtttcatc attacgttgt ttcagctgtt tgtcattatt
    16801 tttgcagttg attggtattt attagtggga aagattggag cttggtatct gattcctttt
    16861 ctattgtgtc tgttgctttt tgtatcccat agttttttta gtacgctaat tgatatctgc
    16921 aaagaagata aagcagatag tcttgtttat ttgatcaaaa ggaatatctg gctgttgtca
    16981 aaaaacgtca aggggtttct gtggtattta tgccttttgt atggactgtt ctggtgtata
    17041 cagcatgatt ttttactcac tgcattactg tatccagggc tgccatttgt cacagtattt
    17101 tgcttaaaag aaaaggagaa tgactttaaa tgaagaaaaa atatgtacaa gtcggacttg
    17161 gtgggagagc gcgttttttt tatgaggcaa ttgcctctac gtatcatcaa acttgtgaac
    17221 tagtcggttt ttgtgatgta tctcaaacaa ggatggatta tgcaaatcgg atacttgtga
    17281 acaagtatgg acagaaaaaa gtaaagacat gtatgccatc acaatttgag caactactgg
    17341 atgacgtacg gccggctgaa gtcattatta cgtcagtaga tcgaacgcat catgattata
    17401 tcattcgtgc aatgaaaaaa ggatatgatg ttatttgcga aaaacctctg actatcgatg
    17461 aggaaaaagc tcaagaaata ataacggtcc aaaaacagac gggaagaagc ctgcgagtca
    17521 cttttaatta tcgttatgca ccgtatcaca cgaagataaa ggaactgata gaaagtgatg
    17581 tgattggtga tgtgttttct atccacttcg aatggctgtt aaatacgcaa cacggggcag
    17641 attatttcag aagatggcat cgaaataagc acaatagcgg cgggcttctt gttcataaat
    17701 ctacccatca ttttgatcta gtgaattttt ggttgaacag ccgtccccaa aaggtatttg
    17761 cattcggtgg attaaacttt tatgggatgc ataatgcgga gaaacgcgga gtagaaatat
    17821 tttacagtcg atgctatgga agcgaactag ctaaagctga tccatttgct atagacctgt
    17881 cagccaatga ggaattgcga gaaatgtatt tagaagcaga aaaagacagt ggatatattc
    17941 gtgaccgcag tgtattttcc gatgatatct ctattgaaga tacactaggc gtaacggtca
    18001 aatatgaaac tggtgcgatt ttgacgtatt cgctaaacgc gtatatgccg tgggaaggat
    18061 tcaatgtttc tttcaatggc agcaaaggac ggatcgaaat gaaagtgatc gaaaagtctt
    18121 acatcaatgc aggtggagat aaaaaggatg aaggagcagc cgttcttcag tccatccatg
    18181 tctatccgat gttcggtgag ccatatgagg taatcgtcga acaagaagcg ggcggacatg
    18241 gcggaggaga tccgcgttta ttagatgatc tatttggtga agaaaaagaa gaagatccgt
    18301 ggaatcgggc agcaactcac gtagatggta ttttgtcgat tttgacagga atcgcagcaa
    18361 atcattctat tgcttcagga aaagcagtag tgattgacga gcttgtcagc ttaaaagaat
    18421 aagaatagat ttacggaaat tacttcccgt agtttataaa atcttgcctt attgccgata
    18481 ggctgtcttt tgaacactat ttattcggaa aaaggggcta tgacatgctt tttgtcatgc
    18541 cttcttttta tgtgggctgc ttttttagat aggagaaaat aaaaataact atttaaaccc
    18601 gaacaaaaag gaataatgca ttaaaaatca ttagatttta caaaaaaata agtaaaccac
    18661 gttgtcagtt tcagaaagta gtgttagact gttttcaata tttgaaacaa cattttttat
    18721 tcaaatatgg caaactgcct tcacgtgcaa aacagttgca agaaaatgaa tttacactat
    18781 tattagtttt acatacctac ttatatatcg tcataatatg gatgacagtt tctagccagt
    18841 accgtaaatg ctggactata agtaaaagat tggctatttc acccttcttt tcttatgata
    18901 aagtggaggg tgtttttctt taacagaaac atagtaggtt tttgcacaaa agcggttatt
    18961 tcggtattaa taaatgataa aggagcaaat aaagtgaaag aattagttga acgtattcaa
    19021 aaagacggac gtatattagg cgagggcgta ttaaaagtcg acagcttcgt cactcaccaa
    19081 gtagaccctg tattgatgga acaaatggga aaacggtttg cagaagtatt taaagaacaa
    19141 ggaatcacga aagttgtgac cattgaagct tctgggatcg caccagcttt atttgctgca
    19201 agagaattag atgttccaat gatcttcgct agaaaagcaa aaagtttgac gatggatgaa
    19261 gaattattga cagcttctgt ttattctttt acgaaacaag tgacaagtac gatttctatt
    19321 tctcgtaaat ttttatcttc agaagacaaa gtattgatta tcgacgattt cttggcaaac
    19381 ggacaagcgg caaaaggatt gatcgaatta tgccagcaag ctggagcgca agtagaagga
    19441 atcgggatcg tcatcgaaaa gtctttccaa gatggacgtc agttacttga agaaatggga
    19501 ttgagagtcg tttctcttgc gcgtattgct tccttgacga atggacaagt tgagtttctt
    19561 gaggaggacg cataatcgtg gaaaaagaaa cacaaaatgg aaaagctgcg gttttagggc
    19621 tgcagcatct gctagcgatg tatgcaggag cagtagcggt gcctcttttg atcggtacag
    19681 gactaggctt taatcaagaa cagatgactt atttgatttc aattgatatc tttatgtgtg
    19741 gattagcgac attattgcaa ttgacagtta accgattttt cggaattggc ttgcctgtcg
    19801 ttttaggatg tgcgatccaa gcagtagctc cactgatctt gatcggaacc aatgaaggag
    19861 taggagctat ctacggatct attatcgctt ctgggatctt tgttgtgtta gtttctggtg
    19921 tcttttctaa aattaaaaaa ctattccctc ctatagtaac aggaacggtt atcaccgtta
    19981 ttggtttgac tttgattcct gtagcaatcg agaaaatggg tggaggtaat gcaacagcag
    20041 ctgattttgg tgacaaaaca aatctgctac ttgcatttgt gacgattctc ctaattgtag
    20101 gcgtgcaaat gctggcaaaa gggttcattc gttcaatcgc ggtattgatt ggtttagtcg
    20161 gaggaagcat cttagctgct ttcatgggaa tgattgatct gagcgcgatt ggagatgcgc
    20221 cgttgttcca tgttccacaa cccttttatt ttggtaaacc aactttcgac gtatggtcga
    20281 ttttactaat gatcatcatt tccatggtca gcatggtaga atcaacaggt gtatattttg
    20341 cacttggtga cattactggg aaaaatgttg gagaagaaga attgaaaaag ggatatcgtg
    20401 cagaaggact tgccgttatc ttaggcggta ttttcaatac attcccttat actggctttt
    20461 ctcaaaatgt cggattggtt caactttcgg gaatcaaaac aagaaaaccc atctatttct
    20521 ctgcattctt cttgattgtt ttaggattat tccctaaaat cggtgcgtta gcacaaatca
    20581 tccctgagcc ggtacttggt ggaggaatgt tagtcatgtt cgggatggtg gcagtccaag
    20641 gaatccgtat gctgttggaa gttgatttca gcaatgataa aaatctattg attgttgctg
    20701 tttctgttgg atttgggctg ggtttcaatg ttacgccgac attattcagt caaatgccgc
    20761 aaacattgca aatgttcact ggaaatggca tcgtaatgag tagcattacg gctatcatat
    20821 tgaatttgat ctttaatgga ttgaaaaaag atgaagaaat attggaagaa gccttcaaga
    20881 agtaatagag caaaagaaag gttgtgaaat cagcgttttg acctgattgt tagagaaaca
    20941 aacggaaaaa tagttcctcg tattttgccg tttgtttgag ctaataccga gggtcagctg
    21001 attgaacacc gttgaaggaa gaggtcgtga gcctgccgtt catctccgag tcacaaggaa
    21061 caatttcaaa aaaaagcttc tcatgtttgt tgaatattgt tccttgtgac cgaggagttg
    21121 gcaggagaac accgtttatc cataaaaagg aatgtgacct aacttttgtc acattccttt
    21181 tttttatctt taataaacac gaacaataaa atgtttttct ttgttaacat tcgaaatttc
    21241 tttgacatca aaaaaagtga ttgttaaaat atgaatgtag aaaaactggg aggaaaaaca
    21301 atgacagcaa aaatctctgt gattatgggg agtacttcag attgggaaac aatgaaagaa
    21361 gcatgtcaaa tattggatga attcggtgtg tcttatgaaa aaaaggtcgt ttctgctcat
    21421 cgaaccccag atttgatgtt tgaatatgca caaaacgctc gaaaaaaagg aatcaaaatc
    21481 attattgcag gagcaggggg agccgcgcat ctgccgggta tggtcgcatc aaaaacaact
    21541 ttgccggtta tcggtgtacc ggtccaatca cgaacattaa atggtttgga ttcgcttctt
    21601 tcgattgtcc aaatgcctgg aggagtacct gtagctacta cagcaatcgg aaaagcggga
    21661 gcaattaatg ctggactact tgcgatacag atgctttcca tgtatgattt agaattggag
    21721 gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg ttatagaaag tagtgatcaa
    21781 cttggttaaa cctttgttgc ctggttcaac gatcgggatc gtcggtggcg gtcaattagg
    21841 tcgtatgatg gcgattagtg caaaagaaat gggctttaaa gtaggtgtgc tggatcctgt
    21901 gagcgattgt ccagccgcac aagttgcaga ttggcacatt gaaagtgtct atgatgacac
    21961 ctttgcgctg aaagagcttg ccagaagaac agatgtcatt acctatgaat tcgaaaacgt
    22021 cagtgtagag gcgttaaatg cgattttacc catgtcattt attcctcaag ggacagactt
    22081 gcttgcgatt acgcaagacc gcctgttaga aaaatctttt ttggaaacga ataacatcgt
    22141 gattgctcct tatgcaacga tcgtcagtcc gacggatatc caagatgcga ttgacgggat
    22201 cggctatcct tgtgttttga aaaccactcg aggcggctat gatggcaagg ggcagtatgt
    22261 tttgaaaagt cgggcggatc tagctcctgc catggacctt ttaagagaag gaacctgtgt
    22321 tttagaagca tggatcccgt ttgaaaaaga gatttcgatc atggtggcag gaaatggtca
    22381 aggagatttc acgacttttc cggtcgtgga aaacatccac cacaacaata ttcttcatga
    22441 aacgattgct ccagccgcga ttgatcagga tgtgatcgag gaggctgagc gtattgcccg
    22501 tgtgattgca gaagcagtga gcctttcagg tgttttaggg gtagaaatgt ttttaacaaa
    22561 aacaggcggg ctatatgtga atgaattagc tcctcgtcct cacaattctg gacattattc
    22621 tattgaagct tgttctatga gtcagttcga tgcccatatt cgaggtattt gtggttggcc
    22681 attaggagaa aaagtacgtt tgataagcaa tgcagtaatg gtcaatgttt tgggagaaca
    22741 actgctggaa agctatcgct tgattgagaa taagccagac tggcaattcc atttctatgg
    22801 aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc actatcttga cagaagacat
    22861 ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg gattagagga gaaaaaacat
    22921 gttagaacgt tatacacgcc ctgaaatggg gaatatctgg acggaagaga acaaataccg
    22981 tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg gcagaacttg gtgaaatccc
    23041 gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt gatatcgatc ggatcttgga
    23101 aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca cgagcggttt ctgaaacatt
    23161 gggagaagaa agaaaatggg tccattatgg tctaacaagt acggatgtcg tagatactgc
    23221 ttatggctat ctcatcaagc aagcaaatga tattttacgg gaagacttga aacgttttac
    23281 ggaaatcatt ggcgaaaaag caaaagaaca caaatataca gtgatgatgg gtcggacaca
    23341 cggtgtccat gctgaaccaa ctacttttgg attgaaactt gccacatggt attcagaaat
    23401 gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga gtagaagcag gaaaaatctc
    23461 aggagcagtg ggaacgttcg caaatatccc gccatttatt gaagagtatg tatgtaaaca
    23521 attggggatc cgtccacaag aaatctctac tcaagttctc cctcgggatc tacatgctga
    23581 atatttctca gcgatggcgc ttattgctac aagtattgaa cgttttgcaa cagagatacg
    23641 cggattgcaa aaatccgaaa cacgtgaagt agaagaattt tttgcgaaag gtcaaaaagg
    23701 ttcttctgct atgccgcaca aaaggaatcc gatcggttca gaaaatatgg ctggtctagc
    23761 cagagtggtg cgcggacaca tggtcactgc ctatgaaaat gtcacattat ggcatgaaag
    23821 agatatttct cattcttcag cagaacgaat catcttgcct gataccacga ttttattgaa
    23881 ttacatgtta aatcgttttg gcaatatcgt taaaaatcta acggtctttc ctgaaaacat
    23941 gaagcggaat atgtctgcaa catttggttt gatttatagc cagcgcgtta tgctgaaatt
    24001 gatcgataag ggaatgacaa gagaagaagc ttatgatctt gttcagccaa aaactgcgta
    24061 cgcttgggac aaccaagtgc agttccgccc gctgttagaa gaagatccaa ccatcacatc
    24121 ggtattatct aaagaagaaa tagacgatgc ctttgattat catcatcatt tgaaaaacgt
    24181 tgacgtgata ttcaaacgag taggtttgga ataaagaaga gtgagttgca aaattcaatt
    24241 aaaatagaat agcaaaaata atcgagtaga ggggaaagcc aactgacctt ccgcccctct
    24301 cacaccaccg tacgtacgga cccgtatacg gcggttcaat aagttgagtg tacgaactgg
    24361 tacttcaagg agatgtcctc atatccttgg cgggccagtt ctttgtctgt taatgatcga
    24421 tgaagaatat gacttttgga gactgaccaa ataccttttc ttgtatttga ccattcatac
    24481 gctttcgctt tatcgatccc taaacgaatc agattttttc ttttggttct tggattcttc
    24541 cattgtttcc aaatatattg tctgattctt cgcttcaacc aaccgtttaa ctccctcatg
    24601 aattctttca tctcaccaat tccatagtag ttgatccaac cagtcattag ttgcttgatt
    24661 tcttttaaaa taagttggat acttcttcca cggtttcttt tagtgagttg tttgagcttg
    24721 cttttcacac gctgttttgc ttttgcgtga gggcgaattt taataccttt ctttgttggc
    24781 agaatgcaga atccaagaaa ctttcgtttc aatggacttc ctaccgcact cttttctcga
    24841 tttaccgtca actttagttg ttcttcgaga aattgaatgg atttcttcat cacacgctgt
    24901 cctgctcgtt tgcttctaac atagatattg cagtcatcgg cgtatcgaac aaatttatgt
    24961 cctagatttt ctagcacttg atcaaattcg ttcaggtaga tgttacttaa caggggagag
    25021 agattgcctc cttggggcgt tccttcttca gttgttgtga tgagtccatt ttccattaca
    25081 ccactcatta agtattgacg gatcagtttt aggacttgtt tgtcctttac tttccgctca
    25141 atgaaataca tcagcttatc gtgatttacg gtgtcaaagt aggctttcat atcaatgtcc
    25201 accacatatc ggtaaccttg ttcataataa gattttgctt tcttcactgc gtcatgtgcg
    25261 ctgcgatttg ggcgaaaccc ataactatta tctgagaatt ctggttcaaa tagcggttgt
    25321 aacacttgat tgattgcctg ttgaagcata cgatcaataa ccgtgggaat tcctagtttt
    25381 cttacaccgc cgtttggttt cggtatttct actcgcaaaa caggctgagg tttatagttc
    25441 ccttggcgta aattggatag taattcctcg cggtgctctt tcaagtaagg aagcagttga
    25501 tcgacggtca tcccatctgc accagccgcc cctttatttc tgactacttg aagatacgct
    25561 tgattgagat tattccgatc tagaattctc ccaaacagat tttctacacc atcttgtttc
    25621 ctctgttcac cgcaagaagt gctgcgcact tcatcatacc cttcgtgttc cacactagct
    25681 ctttgatgat agccaagttt tcttgttttc tgcggtcgtc gcatcttctc acacctccta
    25741 aattattcaa gattcttatt gttcagtcct tcgtaacggt cagtgtcgtt actactatga
    25801 cctcggctga cttctcatga ttcaccatca tgtcgccatg atcgttgtgc tggtttgttt
    25861 tcattcaacg cccacaacac cctcatgaga tctcccccgg taagagtgaa aactttcctc
    25921 ccatgtaact gctagattta ctgtataggt ttcgggcagt attggacttt gtcttgtgat
    25981 gaagacttat ccgacctaat tcagccttgt atctagtttc tgttcgtcag ttcaggagtt
    26041 tgcgtccgac ttccttcagc cacaacctca cgattgccgc cttgtctttc gctaacagtt
    26101 cctactgcca agcctgtagt ggactttcac caccaagttt tcacccatgc agggcgcaca
    26161 ccaaaaaaga agcagatccg ttagcggttc tgcttctttt agtttattat attttcttat
    26221 caataatagg aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa
    26281 aaaatagagg aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag
    26341 atgttgcagc aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg
    26401 gatacgataa tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc
    26461 tttgtgttct gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc
    26521 tcggcggatt tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt
    26581 gatcgacagc aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa
    26641 aagctgacct tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca
    26701 aatttttagt taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt
    26761 ataagatttt cgtttctgat aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac
    26821 atatttttgt tgatttataa aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa
    26881 aaacgatgta tttgctttta atgtaaataa aaaggagaga cgtacatgga gaagaaagaa
    26941 cttttgtatg aaggaaaagc caaaaaaata tatcttactt ctgatgagaa cttgatttgg
    27001 atagaatatt tagatcaagc aactgcgttg aatggcgcga ggaaagacca gataagtgga
    27061 aaaggagaat tgaataatca aatcacaagt gcaattttcc gttatctgac tcaaagagga
    27121 ataaaaaatc attttattga acaattgtct aaaaccgaac aattagcaaa aaaagtacaa
    27181 attatccctc tggaagtggt tatccgaaat attgcagcag gaagtttctc aaaacgtcta
    27241 ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg aattctacta taaggatgac
    27301 cagttggatg atccatttat caatgaagag catatctatt tcttacaact tgcaaagccg
    27361 gaagaaatca gtgagctgaa agcccaagcg ttggctatta atgaaatttt gaccgaatta
    27421 tttgctaagg tcggtttggt tttagtagat ttcaagctag aatttggccg gtcgaatgga
    27481 caaattttgc ttgcggatga agtaacaccg gatacttgcc gattatggga tgaacgtacg
    27541 aatgaacata tggacaaaga cgtttaccgc agagatctag gtgatttgat cccagtctat
    27601 caagaagtat ataaccgtct atcggaacta actgaagaaa aggagcgagt atgatgtatt
    27661 tcgtaaaagt atatgtaacc tataaagatt cagtgttaga tcctcaaggg gaggcagtaa
    27721 aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga tatccgaata ggaaaatact
    27781 ttgagataca acttgctgat gcagaagaac caatcgatac agtgattgaa acgatttgtg
    27841 acaagctgct tgcaaatgta aatatggaaa cataccgata tgaaatcaaa aaaatggagg
    27901 aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga cgcagatatg
    27961 ttatgggcag ttcgcgagat catgggcgct gatgccgaat ttgtacggca tgatgagtcc
    28021 tctttagaag gatttgatgg ggttttactg ccgggaggat tctcctatgg tgactatttg
    28081 agatgcggtg cgattgctcg gttttctaca atcatggaag aagtgattcg ttttgctgaa
    28141 gaagggaaac cagtattcgg tacatgcaat ggattccaga tattgacaga agcaggactt
    28201 ttgccaggtg tactgagacg aaatgagtct ttgcatttta tttgcaaaac tgtaccattg
    28261 aaagtcaacc ccaagacggc ctttacttca gagtatgaag ccgacgaaat aatctatctt
    28321 ccagttgctc atggggaagg aaattatttc tgtgacgaag aaacacttca agaactaaaa
    28381 aaaaataatc agattgcctt tacttataat gacaacatca acggcagcgt ggaaaacatt
    28441 gcagggatta cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc agaacgtgca
    28501 gtagaagact tgctaggatc agaagatggg aaacggttct ttgcttccat tttaaaaaac
    28561 ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac cgacagctga agagatcaag
    28621 aaaaaaagaa tctatgccga atggggattg actgatgaag aatatcgaat gatcgaagaa
    28681 gatatcttag gaagaatgcc taattatacg gaaactggac tgttttctgt tatgtggagc
    28741 gaacattgtt cctataaaaa ttctaaaccg gttctacgca aatttcctac tagtggacct
    28801 catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg acatcggtga tggactagca
    28861 gttgtcttca aagcagaaag tcacaatcat ccttcagccg tggagcctta tgaaggagct
    28921 gcgacaggag ttggcggaat catcagagat attttcagca tgggggctcg tccaattgcg
    28981 ttgctagatt cattgaggtt cggcgaatta gataatccaa gaacgaaata tttactagaa
    29041 gaagtagttg caggtatcgg cggatacggc aattgtatcg gcataccgac tgtaggcggc
    29101 gaaattgcat ttgatccttg ttatgaagga aacccattag tcaatgcaat gtgcgtaggt
    29161 ttgattgaac acaaacatat ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg
    29221 tatgtaggtg caaaaacagg tcgcgacggt atccacggag caacttttgc ctcagaagaa
    29281 tttaatcaag aagaagaaca gcaacgttct gctgtacaag taggcgatcc ttttatggaa
    29341 aagctgctgc ttgaagcgtg tttggaatta atcttgcatc attcggattc tctaataggt
    29401 atacaggata tgggagcagc aggacttgtt tcatctagtg cggaaatggc ttcaaaagct
    29461 ggctcaggac ttatattgac attagatgaa gtcccacaaa gagaaactgg gatgacacct
    29521 tatgaaatga tgttatcgga atcacaagaa cggatgttga tttgtgttaa atcaggagag
    29581 gaagaaaaaa tccaagaact atttcagaag tatgatctag atgcagtcac tatcggtaaa
    29641 gtaacagacg acgggcaata tcggctatat catcaaggag aagaagtagc aaatctgcca
    29701 gtcgatgcat tagctgaaga tgcaccagtt tatcataaag aaatgaaaga accagctcga
    29761 atcgtagaat ttcagcagtt agctccttat cagccagtta tcgaagaacc tggacaagta
    29821 ttgcttgatc tgctgcagca accgacgatt gcttcaaaac gttcaatcta cgaaacttat
    29881 gattcacaag tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg
    29941 gttagaggga cgaataaagc gttagctatg accacagact gcaacgcgcg ctatctgtat
    30001 ttggatccaa aaatcggagg acaaatagca gtggcagaag ctgctagaaa tattattgct
    30061 agcggtggta agcccttggc gatcaccgac tgcctgaact atggttctcc tgataaacca
    30121 gaagtttttt gggaattatc gacttcagct gacggaattg ctgctgcgtg tgaaacgtta
    30181 ggaactcctg ttatttcagg aaatgtctct ttatataatg aaacggatgg tcaagcaatc
    30241 tatccaacac caatgattgg tatggtcgga ttgattgagg atcataaaca tatcactact
    30301 caggaattca aaaaatcagg ggatctaatc tatatattag gtaagacgtt tgcagatttt
    30361 gatggcagtg aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt
    30421 gatctatcca ttgaaaagag aaaccaagag ttagtattga cagccatcca aaacggattg
    30481 attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc agaatcagct
    30541 ttcaaacatc agctagggat atctgtacag tttgaattgt cctctgctca attatttgca
    30601 gaaactcaat caagatttgt gttgacagtc gcaccggaaa ataagacacg attcgaagaa
    30661 atgatgggag atgctgctgt tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt
    30721 tcagcaactg acggtcagat caaaatagaa actgcagtag caagaaagtg ctgggaggat
    30781 gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt
    30841 ggggtcatcc agacgccgct agtgtggcat acttcgggct ccatagtctt cagcatcgag
    30901 gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg catcgagatt
    30961 tggggctgct ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta acaggagaag
    31021 ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg aagtgtggac aatattcagc
    31081 cttttctatt caaatttcat gatcagtcga tcggcttagc gcataatggt aacttgatca
    31141 atgctcgttc ccttagaaaa gagttagagg aaaatggggc gatcttccat tctaactccg
    31201 atacggagat attgatgcat ttgatccgac gaagtgaaga aacagatttt ttaaataaat
    31261 taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta tttattaatg acagaaacgg
    31321 caatgattgc cgctttggac ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa
    31381 acggagcata cgtgattgcc tcggaaactt gtgcattaga agttattggg gcacgtttta
    31441 taagagatgt ccagcctggt gagatagtga tcatcaatga cgaaggaatc cagattgatt
    31501 gctttacgaa ggacacgcag ctttctattt gttcgatgga atatatctat tttgccagac
    31561 cagattcaaa catcgcaggg atcaatgtgc atactgcacg gaaaaatatg ggacggaatt
    31621 tagcgaaaga atcgccagta aaagcagata tggtcgttgg cgtacctaat tcttctttgt
    31681 cagccgcaag cggttatgcg gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa
    31741 atcaatacat tgcccgaaca tttatccagc caacgcctga gctaagagag caaggtgttc
    31801 gaatgaaatt atctgctgta cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg
    31861 attcaattgt tcggggtacg acaagcagac ggatcgtcca tcttttgaaa gaagcagggg
    31921 caaaagaagt ccatgtgagg attgcttctc ctcctttgaa atatccatgt ttttacggga
    31981 ttgatattca aacgcgtaaa gaactcattg cggcaaatca cacgattgat gaaatcaaag
    32041 aatgtatcgg tgcagattct ttaggttttt taagtgaaga gggtctgatc gaatcgatcg
    32101 gtctggaaaa agatgctccg tattcaggat tgtgtatggc ttatttcaac ggagattatc
    32161 cgacgccgtt atatgactat gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt
    32221 tcttttaaaa gcagaccaag aagggagtat atgaattcaa tggcaaatgc atacgcaaaa
    32281 gctggcgtag atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa
    32341 aaaacggaac gtttaggtat gatgggcgct ataggaggat tcggcggttg ttttgattta
    32401 tcggtctttc aattaaaaga gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa
    32461 ttagcagtgg caatcgaagc agacaaacac gatacaatcg ggatcgactg cgtagcgatg
    32521 tgtgtcaatg atatcttagc acaaggcgca gagccgttat atttcttaga ctacttagca
    32581 acggggaaaa atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc agatggatgt
    32641 gttcaaggaa atatggcgct gattggcgga gaaacagctg aaatgcctgg tatgtactca
    32701 ggtaatgatt atgatctagc cggatttgct gtagggatag ctgaaaaatc tcaattattg
    32761 acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa tagcatctag cgggattcat
    32821 tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc agcagcagtt ttctttatca
    32881 tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc
    32941 tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc
    33001 actggtggag ggtttgtaga aaatatcccg cgaatgttac cagaatctat ggctgcacaa
    33061 atcaaacttg gctcatggcc gattcttcca atattcggct gtttacaaaa atatggtgaa
    33121 attcctgaga tggagatgta tgaaatcttc aatatgggca tcggtatggt cctggcagtc
    33181 caagaatcga aagtaaaaga aatagtacac gaaattgagc aagctggcga aaaagtttat
    33241 gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg agaagaacga
    33301 tgagaatcgc tgtttttgct tctggaaacg gaagcaactt tcaagcgcta gccgattatc
    33361 tatcgaaaaa aggattggaa tcttcgattg actggttgtt ttgtgatcag cctgaagcat
    33421 atgttttgaa acgtgcaact gcgttgagtg taccggcaga ttgtttttca cctaaagaat
    33481 ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg
    33541 atttgatcgt attagctgga tatatgcgaa tcattggacc ggtattgctg aaaaattatg
    33601 ataaaagaat catcaatatc catccgtctc ttttacctgc cttccctggt ttgcatggca
    33661 tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt cacaattcat tatatcgacc
    33721 aaggtgtaga tactggacca atcatccggc aggaaaaagt gagaattgaa caggaggaca
    33781 cgttcgattc tttagaagaa aaaatccata gagtagagca tcgaatctat ccagaagtga
    33841 ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca agagcattga tcagtgtatc
    33901 agataaaact gggattgtag cattcgccca aggtttgatt gaacaaggag tagaaatcat
    33961 ttcaacagga ggaacaaagc atgcgttgga taaagctggg atcaaaacgc tttcgatcga
    34021 agaagtgaca gggtttcctg aaatgatgga tggccgtgtc aaaacacttc atcctaaaat
    34081 ccatggtggt cttttaggaa gacgcgatct ccctagccat atggaggcaa tggaaacgga
    34141 aaatatccaa ccaatcgact ttgtttgtgt caatttatat cctttcaaag aaacgattat
    34201 gaaaccagat gtcgaaatag cagaagctat tgaaaacatt gatatcggtg gtcctagtat
    34261 gctccgtagt gcagcaaaaa accatgaatt tgtcacctct gttgttgatc catctgacta
    34321 tgaagaagta ctttcagaat tacgtcagta tgggcatact tcactttctt tcagacaaaa
    34381 actagcggca aaagtctttc gtcatactgc ggcatatgat gcactgatcg ctcaatactt
    34441 aacggattgg gtggaagaaa aagaaccgga aaaattgaca cttacgtatg aacgaaaaca
    34501 aacgcttcgt tacggtgaaa atagccatca agccgctgcc ttttatcagt ccgctcttct
    34561 ggaaaaatat tcgatcgcag cagcaaaaca actccatggg aaagaacttt cttataataa
    34621 tatccgagat gcagatgctg cgctacggat catgaaggaa tatgacgagc caacggtagt
    34681 cgccttgaaa cacatgaatc cttgtggtat tgggacagca tctacgattt tagaagcgtg
    34741 ggaagcagcg tatgaagcag atcctgtttc aatctttggt gggatcatcg ttttgaatcg
    34801 agaagtagat ctgccaacag cagaacagat gcataagctg tttctagaaa tcatcattgc
    34861 cccttcttat acacaagaag ctttggaatt actgcaaaaa aagaaaaaca tccgattact
    34921 gacagtagac tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct cagtcttagg
    34981 tggacttctt gtacaagatc aagatctggc aatagaaaat gaagaagagt ggaaagtcgt
    35041 gacaaaacgt aagccgactc cagcagaagc aaaagcatta gcgtttgctt ggagagctgt
    35101 caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg
    35161 agccgggcag atgaatcgag tcggttcagt aaaaattgcg attgaacaag cgcaagagaa
    35221 aatggaaggt gcagttttag ctagcgatgc ttattttcca atggatgatt cggttgaata
    35281 tgcagccaaa catggtataa aagcaatcat tcagccagga ggaagcatca aagaccaaga
    35341 ttctattgat atggcgaata aatacgatct agcaatggtc tttactgatg ttcggcattt
    35401 tagacattag agaggagtcg gataaatgaa cattttggtg atcggtgcag gtggtcgtga
    35461 acatgcaata agtaaaaaac ttttagccag tccgaaggtg gatacggtgt attgtgcgcc
    35521 aggaaatccg gggatgacaa aagatggtat caattgtgtc gctatcagtg agcgagatca
    35581 ttcagcgtta atccattttg ttaagaaaga ggctattgat tggagtatcg ttggtcctga
    35641 agttcctttg ttaaatggga tcgtcgatga ttttatggcg gaaggattga aaatcttcgg
    35701 tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga
    35761 tacttatcaa atcccgacag ctaaatccag aagttttgaa tgttacgaaa aagcagcagc
    35821 gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag cagctgggaa
    35881 aggtgtagtc gtggctacca cagtgaagga agcgctggat gcggcagaac aaatgttgaa
    35941 ccagcatcga tttggggata gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga
    36001 agaattctcc cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca
    36061 agatcataag cgggcatttg ataacgataa agggccaaat acaggcggaa tgggtgctta
    36121 ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag aagttttaaa
    36181 accagcggct ttgggaatga aagaactagg acgctctttc acgggtgttt tatatgcggg
    36241 tttgattgct acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc
    36301 tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt
    36361 attagagaat cgtacagtag agttaaagtg gcagcaagaa ggatacaatg taggcgttgt
    36421 ggttgcagct gccggttacc ccgaagaata tgagacagga atggttctac cagattttct
    36481 ggaagaagag ctttctgttt actatgctgg agtttcctca aataacggca aactagttgg
    36541 agcaggagga cgtctttatt tagtagaagc atacggtgag acgatcaaag aagcacagaa
    36601 aaaaatttac accgcgttaa tggataaaac gacagatgga acattttatc gtacagatat
    36661 tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa atcagctgct tccatttata
    36721 aaaaatacaa caaacataat cggaagcgcc gattttagtg aagccttttc ataaagagtg
    36781 tggtacactt ttgttgtgta tataaataag gaggagttca gatgaaaaaa attcaattag
    36841 gtacttctga tcagaaagca gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg
    36901 aagatccagt aaaagtgatt gagacagctt atgaaaacgg cattgatttt tttgaccatg
    36961 cagatattta tggaaacggt gaatgtgaag aaatctttgc agatgctcta gcaaaaacat
    37021 caatcaaacg agaagatctt tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt
    37081 ttgatttttc taaaaaacat atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa
    37141 tggattatgt ggatgcttta cttcttcatc gtccagatac attggtagaa ccagaggaag
    37201 tagctgaagc gtttgatgaa ttggaacggt caggaaaagt tcgttatttt ggcgtcagca
    37261 atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca ttagtagcta
    37321 atcagctgca atttggcttg aaacatactg gaatgatcga tcaagggatt catgtcaata
    37381 tgacagatga agcaagtatc gatcatgacg gtagtgtact tgattattcc cgattgcatg
    37441 atatgacgat ccaagcatgg tcaccatacc agtatggatt ttttgaaggt gtatttatcg
    37501 ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga actagcagct aaatatcaaa
    37561 cgacaccaac tggcttggct tctgcttgga ttttacgcca tccagccaat atgcaagtga
    37621 tcgccggtac gatgaacgta gggcgaatca aggaaatcgc tcaagcttca gaaattaata
    37681 tgtctcggga agattggtac gaattgtacc gtgctgctgg aaacatttta ccttaaagat
    37741 tgtcataagc aggtcaaaag ggctataaat taaaaagaca gaataacttg aaagctgttt
    37801 tcagcttcga gatattctgt ctttttttat tgcaaatgga aagagtatca ttgtacggat
    37861 ttcgtttatt agttattttt gtttttaagg aaaagctgtg tccgatattc ttttggagaa
    37921 atcccttgtt ttttcttaaa taaataactg aaatagttag ggtcattata accaatttca
    37981 aaagcaatat ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt
    38041 ctttgttctg tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg
    38101 aaatgagagg gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg
    38161 gtaaaatttt ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt
    38221 gaaggctgtg agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa
    38281 agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct
    38341 tgcgggattt tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa
    38401 cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta
    38461 gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa
    38521 tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg
    38581 gaactggcgg tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt
    38641 ctttctgcga caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca
    38701 tggaccaacg tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca
    38761 actattaaaa atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc
    38821 ctaaataaaa acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact
    38881 tggtttgctg ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca
    38941 gcaagtttca atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga
    39001 ttttttttca attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct
    39061 aaagcagtta ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc
    39121 agataatcat ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca
    39181 ctaataaata tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac
    39241 ccgtccatga aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt
    39301 gaagacagtg ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa
    39361 ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata
    39421 aaaactttat acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt
    39481 gacagttgtt ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta
    39541 ataaagaagg agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc
    39601 gatccctttg gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc
    39661 gttgtctata actttgaatt ggatcatctc atcaattttg catgtggtaa tacgaataaa
    39721 tcctttgtga cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa
    39781 gatcattttt aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag
    39841 catatggccg tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt
    39901 ttcaagagga atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc
    39961 atgggtcata gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc
    40021 caaactgtta taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg
    40081 tgctaaatgg tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat
    40141 tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa
    40201 gccaaggttc cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg
    40261 gacctttctc tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac
    40321 tccaagttcc actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct
    40381 tgctaagtcg atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac
    40441 agaatctacc tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt
    40501 attgatcagc tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa
    40561 cgtactaatt tctttaagtg tatttgtatt atttttgatc tctcgaatat cattcctaag
    40621 ttcttctagg atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc
    40681 ccacatactt tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat
    40741 atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag
    40801 caaagggata acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tctgttttat
    40861 gcttaaattt tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta
    40921 tattctgctg ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg
    40981 tttgaatatt tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt
    41041 tacactcgac tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc
    41101 catcaatagt tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag
    41161 ctaaagcacc gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat
    41221 catttgtttc taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga
    41281 caattcggtg aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg
    41341 ttggtttacg attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt
    41401 tctctgaacc agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat
    41461 tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga
    41521 cgaaaagctc gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg
    41581 ctcgctttgc ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga
    41641 tcatatccac tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca
    41701 tgtagccatt tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt
    41761 cagtatgctt tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg
    41821 ggtcaggtgc ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa
    41881 gggctaaaaa aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact
    41941 atcttatcag ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga
    42001 aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa
    42061 aaaaatcaat gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg
    42121 ataaaaaatg aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag
    42181 cattctatta agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg
    42241 aatcgctatg ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc
    42301 ggaattagaa aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa
    42361 tcaagtagca caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc
    42421 tatcgatggt tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt
    42481 gatcgcctat gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga
    42541 taattttgga gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga
    42601 aggaaaagga ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact
    42661 aatcaattac aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt
    42721 cgtgccatct ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac
    42781 ggcgcaagca aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc
    42841 tgttttgtcg ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg
    42901 gtatgggagt gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg
    42961 cgttaaatcg atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt
    43021 agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa
    43081 tgatactgaa acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt
    43141 atctgtcgat aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc
    43201 cgatctgaaa aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg
    43261 ataagaatta tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga
    43321 atccgagcgt tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc
    43381 ggagaaaacg gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat
    43441 ggctcctatg acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa
    43501 gatagtgaag aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt
    43561 tcaataaaag agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg
    43621 gaactgacag aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg
    43681 aatcttctag tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca
    43741 ttatcaaaat ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa
    43801 gaaagcgcga atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc
    43861 attatttcac ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg
    43921 gatggacaga cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc
    43981 ggaatggtgg gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa
    44041 gtttatttta aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata
    44101 aacgaccaca tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg
    44161 ggagcgggca gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata
    44221 tccggacgta tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt
    44281 gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg
    44341 gatattcggg aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta
    44401 gataaagaaa aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca
    44461 aactccatcc atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta
    44521 tccaagtggc tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata
    44581 gatgttgggg caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa
    44641 gctgtctgtc ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat
    44701 acgatgaacg aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc
    44761 ttgatgaaat taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac
    44821 ggataaaaaa gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta
    44881 cagcatggtg atcattttag cgggactact tattgcgttt caattgatga cggatggtat
    44941 tttctggcgt ccattgaata taactaacct tgttctgcaa aatagccata tattagtact
    45001 agctgcaggg atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt
    45061 gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg
    45121 gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg
    45181 ggtagcttat gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg
    45241 tggactgaca caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca
    45301 aaaaatgtct acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac
    45361 attgatttta ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa
    45421 tcgtcggaaa aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt
    45481 atttacagca gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc
    45541 gattattttg atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac
    45601 agtagcaggt cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg
    45661 gatcaaaaca aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt
    45721 agctggatta gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat
    45781 ggaactggat gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg
    45841 aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc
    45901 gattttaggc gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc
    45961 cgttgtgcta gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc
    46021 agaaaaaaga ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa gcgaacattc
    46081 attgtttcgt ttgtatagac taaaaataat atatgttttt ttaccattcg tataaattct
    46141 ctttttgctc ttgatttttt tgcttaattg agttaaatta catagggcgc taaaaaaagc
    46201 cttgattaaa ttaataatgt tcgtgttttg ctggatgtga acatcaggag gataatgtaa
    46261 tgaaagtatt tgattacgaa gatattcaat taattcctaa taaatgtatc gtaaatagcc
    46321 gttctgaatg tgatacaacg gtcacattag gtaaacatac attcaaaatg ccagtagtgc
    46381 ctgccaacat gcagacgatt attgatgaaa caatcgcaga attcttagcg gaaaatggtt
    46441 atttctatat catgcatcgg tttgatgaag cagctcgaat tccgtttatc aaaaaaatga
    46501 aaaagcgtgg attgattacc tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta
    46561 tagagaagtt ggcagaagaa tcgctaaatc ctgattatat cactatcgac atcgcgcatg
    46621 gacatgctaa ctcagttatt gatatgattc agcatattaa aaaatatttg cctgaaactt
    46681 ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg
    46741 cagatgcaac aaaagtcggg attggtccag gaaaagtatg tattacaaaa atcaagacag
    46801 gctttgggac tggaggctgg caactggcag ctcttagatg gtgtgcgaaa gcagctcgca
    46861 agccgatcat cgctgacggt gggatcagaa cacatggcga tattgcaaaa tctgtccgtt
    46921 ttggtgcgac aatggtcatg attggttcat tgtttgccgg tcacgaggaa tctcctggcg
    46981 aaacaaaagt cgaaaatgga atcgtgtata aagaatactt tggttctgct tcagaattcc
    47041 aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg gatccaacac aaaggatctt
    47101 tgaaagatac actagtggaa atgcaacaag accttcaatc atctatttct tatgctggag
    47161 gaagagatct ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat tcgattttta
    47221 atggagacac aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc
    47281 attaagtaat aagaacagcc aaacaaaaat agttctttat atgttttgtt tggctggaat
    47341 tattatcgca gatacaagct tttgaacatc attattcaga ataagggact gtgataagat
    47401 ttttgtcaca gtcccttatg ttttgcaaaa aaagtttttc ttttttctcg taaattcggt
    47461 aaaatcaaaa gaaaaaatag acaatagaaa agagaagctt atgtgggaaa aagtagagaa
    47521 aaaacaagga ccgatttatc gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg
    47581 aaggatcaat cctggaagtc aacttgaatc agaacgaaaa ctagggcaac tttttggtgt
    47641 gaatcgttcc accgttgtcc atgcattgga agagcttagg gagcttgggg tactaacgag
    47701 caaacgtggc agcggatggt ttgttaatca gactgaatgg gggaaattcg ctgttccaag
    47761 aatcgattgg cggcaaatga tctctcctcg ttatgagcag acggactggt atgagcaaaa
    47821 aataagagaa gcaaagcaga tccaaaaaac atcgtttctt gatttatacg ctagcgagat
    47881 gccagatgag cttttgccga attttgagtt tccttcttat tctcttgaag aaatcttagc
    47941 ggaagaaaaa gaaatggatc ttttaggcta tactccgtta cgtaacaaaa tcaaacggta
    48001 tttggaaagt aaatttgaat tggcattttc ccccaaccag cttcttgtga ctggtggcgg
    48061 tcagcaagca atctttttga ttttacaaac gattctttca gctggagagg ggatagctgt
    48121 agagtcacct tctttttttt accgattacc tcttttcaaa gcttcaggaa cgcgtttatt
    48181 tgggataccg atggatgaag aagggatcga cttagaaaaa ctgaaagaat cgatccataa
    48241 aaataaattg aaagcagtgc ttatcaaccc gaattttcaa aaccctacag gaaaagtgat
    48301 gagtcaaaaa cgacgagaaa agctagtaga attgtgtcga cagtatcggc ttccaattat
    48361 tgaagacgat gtatttacgg atctttcatt tctaaaccaa gaagaagtcc ttcctatacg
    48421 aacgattgat ccggataatg tgttatacgt cggttcgtta tctagggttt taggaaaaac
    48481 aacgaaaatc ggttgggtga taggacctga gacttttatc tcgcaactag ctcaagcgca
    48541 agaaatgatg gagttttcga tgaatatcct tacacaaatc acagtggcta atgtatttaa
    48601 tgaagcaatt gatttgaaaa tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact
    48661 gataagcgct tggggagatt cacaatcgtt tttcaagctt cattcaattt taggtggtta
    48721 ctatgcctgg attacttggg atgggaaaaa actagacccc gaactagcag aaaaagtagt
    48781 agatatgggt cttggaattg caccaagtct actttttggg caagatataa atggaatcag
    48841 aatcaattac agtaggctgg atgaaaacca aataccgttg ttttattcaa agatgaaaat
    48901 tctagaaaag tggttaacag aataatttca caaagtaaat agattaaaga atataacagc
    48961 ttggatttaa atttcaagct gtttcttttt tttcggaaaa agaatacgat ataaagaaac
    49021 taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt aaataaaata aatgtttgca
    49081 acaaaactgt ataacaccaa cgtttttaaa tagaaacaga tggttgtaag tttgattaga
    49141 aaatagaaaa aatgcagatg aaatataaaa cgctttcctt attgtgattt tattcactaa
    49201 tgctgttaat atgcggattg gcataatttc ctttcatcgt gaaaatttgt gcttttagct
    49261 tgtatttctc ataaatccat gataatttat taatgaagac ggttaaatag tgatacagtt
    49321 ttattcactg tttcaatcgc agaatgaaga gattttctta tagatgagag aggaatgtgt
    49381 gacaaatggc aaacaaaaaa gcaccgatcg acttccaagc actgctagat gaagtcaatg
    49441 cagatttccc agtatatcaa gcacttgatc aagatggaaa cgtagttaac aaagatttag
    49501 tacctgattt atccgatgat gaactcgttg aattaatgac tcatatggta tggtctcgtg
    49561 tattagacca acgttcaaca gcgctgaacc gccaaggacg cctaggcttt tttgcaccaa
    49621 ctgctggaca agaggcaagt cagctggcta gtgcttacgc atttgataaa gaagatgttc
    49681 ttcttccagg ttatcgggat gtacctcaac tagtaaaaca tggtttacct ttatctcaag
    49741 cttttttatg gtctcgagga catgctgcag gtaattctta tccggaagaa ttgaaagctc
    49801 taccaccaca aattatcatc ggtgcacaat atgtacaagc agccggtgtt gcattaggtt
    49861 tgaagaaacg aaacaagaaa aatgtcgtct tcacttatac tggtgatggc ggttcatcac
    49921 aaggtgattt ttacgaagca atcaatttcg ctggcgctta ccatgcaaat gccgtatttt
    49981 atatccaaaa caacgggtat gcgatctcta cacctcgtga aaaacaaact gcagcaaaaa
    50041 cacttgcaca aaaagctgtc gcagctggga ttcctggtat tcaagtcgat ggaatggatc
    50101 cgcttgcagt atatactgta tctaaaatgg cacgggaatg ggcgatcagt ggaaatggac
    50161 cagtcctaat cgaaacactg acttatcgtt atggaccaca tacactatct ggtgacgatc
    50221 caacccgcta ccgtacaaaa gacacagatg acgattggca gaaaaaagat ccattgatcc
    50281 gcttccgtaa atatttgacg gaaaaaggac tttggtctga agaaaaagaa gaacaagtca
    50341 tcgaagcaac taaagaagaa atcaaagcag ctattgctga agccgacaaa gtaccaaaac
    50401 aaaaggtttc agatttcttg aaaaacatgt tcgaagtatc tccacaatca atcaaagaac
    50461 aaattgcaat ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa caatgatcca
    50521 agcaatcaca gatgccttag cacttgaact agaaaatgac gagaatgtcg ttgtttttgg
    50581 tgaagatgtt gggaaaaacg gaggagtttt ccgggcaact gaaggattgc aggaaaagtt
    50641 cggagaagac cgagtattcg atactccttt agctgaatct ggaatcgcag gattgagttt
    50701 cggtcttgcg ttagaaggat ttcgtccagt tcctgaaatc caattctttg gttttatttt
    50761 cgaagcaatg gacgaagtag tagctcaaat ggctcgtaca agatatcgaa tgagcggcac
    50821 tagaaatcta cccatcacaa ttcgttcgcc atttggtggc ggtgttcata caccagaatt
    50881 gcattcggat aatttagaag gtttgatcgc tcagtctcct ggtattcgag tagtgattcc
    50941 ttccaaccct tatgatgcaa aaggactatt gatttcttct attagaagta acgatccggt
    51001 tgttttccta gaacacatga aactatatcg ctcattccga gaagaagtgc cggatgaagc
    51061 ttacgaagtt cctttggata aagcagcagt tactcgtgag ggaacagatg tatctattat
    51121 cacatacgga gcaatggtgc gtgaagcaat caaagcagca gacaatcttg caaaagaaaa
    51181 tatatctgtt gaaatcattg atcttcgtac agttgcgcca ttagatgtag aaacgatcat
    51241 tcaatccgtg gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac gacaagcggg
    51301 cgttgccgcg caagtagttt ctgagatctc agaacgcgct atcctttcat tagaagcccc
    51361 aatcggacga gtttctgcac ctgatacagt cttcccattt ggacaagcag aaaatgtctg
    51421 gttgccaaat gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat tttaagaaaa
    51481 gaagggacga aacaaagtca tggcttatca atttaaattg ccggatatcg gtgaaggaat
    51541 cgcagaaggt gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga
    51601 tacattacta gaagttcaaa atgacaaatc agtagaagaa atcccgtcac ctgttacagg
    51661 aacggttaaa aatgtgattg ttccagaagg taccgtagca aatgttgggg atgtattagt
    51721 agaaatcgat gcaccaggac acgaagacaa tgaaggggac agtggtgtag ccgcagaatc
    51781 ccaaactcca gctaaaccgg ctgctgaacc cactgttgat acagaatcag ctggatcgtc
    51841 aagcgaagga gtcttccaat ttaagttacc ggatatcggt gaaggaatcg cagaaggtga
    51901 aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga
    51961 agttcaaaat gacaaatcag tagaagaaat tccgtcacct gtcacaggga cagttaaaaa
    52021 cgtgattgtt ccagaaggca ctgtagcaaa tgttggggat gtattagtag aaatcgatgc
    52081 tcctggacac aatagtgcac cgtcaactag tgcgccatca gctgaagcac caaaagaaaa
    52141 agtggaaaca tcaggttcag caagtgtcgt tgaagctgcc gatccaaata aacgtgtatt
    52201 ggctatgcca tctgttcgtc aatttgcccg tgaaaaagat gtagatatct cacaagtaac
    52261 tgcaactgga aaaggtggca gagtaacaaa agaggacatc gaaaacttct tggcaggtgg
    52321 tccaagcagt gcaccagcga aatcagaagc gccagaagcg gcagcaccga aagaagcagc
    52381 tcctgcagca gaaagcaaac cagctgcacc agcaaaacca ttcaagtcca acttaggaga
    52441 cttggaagaa cgtgtagcaa tgacgccaac acgtaaagct atcgctaaag caatggtcaa
    52501 cagtaagcat acagctccac acgtgacatt gcatgatgaa gtagaagtgt ctaaactatg
    52561 ggataatcgg aaacgattta aagaagttgc tgctgcaaat ggtacgaaac ttactttctt
    52621 gccatatgtc gtgaaagctt taacagcgac tgttaagaaa tacccagtat tgaatgcatc
    52681 tatcgacgat gcaaaccaag aaatcgttta taaacattac tacaatatcg gtattgcaac
    52741 tgatacggat cacggactat atgtaccaaa tgtgaaagat gcagaccgca aaggaatgtt
    52801 tgctattgca gacgaaatca atgagaaagc aaaattagct catgacggca aactatctgc
    52861 agaagatatg cgcaatggaa caattacgat cagcaatatc ggatctgtag gtggcggttg
    52921 gtttacacca gtcatcaact atccagaagt agctatttta ggtgtaggaa cgattgcaca
    52981 acaaccaatc gtaaatgctg aaggcgaaat cgttgtcggt cgtgtgatga aactgtcatt
    53041 aagtttcgat caccgtatcg tagatggagc aactgcgcaa caagcaatga ataatatcaa
    53101 acgtctttta gctgatccag agctattaat gatggaagga tgataaacaa atggtagttg
    53161 gagattttgc tgtagaatta gatacagttg taattggtgc gggacctggt ggctatgtag
    53221 ctgccatccg cgctgctgaa atgggacaaa aagtagcaat cattgaaaga gagtatattg
    53281 gcggtgtttg tttgaatgtc ggctgtattc cttcaaaagc actgatagct gcaggacacc
    53341 attatcaaga gtcactagat tctgaaatgt ttggagtaac tagtgaaaac gtcaaactag
    53401 attttgcaaa aacacaagaa tggaaagaaa acaaagttgt ccatacgttg acttctggtg
    53461 ttggtttcct attaaaaaaa cataaggttg aaacaatcga aggagaagcg ttcttcgtag
    53521 atgatcacac actacgcgtc attcatccag attcagctca aacttattcg ttcaataacg
    53581 caatcatcgc aacaggttcc cgtccaatcg agattccagg atttaaattc ggaggacgtg
    53641 tgttagattc tactggcgga ttagcattga aagaagtacc taagaaattt gtgatcatcg
    53701 gtggcggtgt gattggtgcc gaattgggcg gagcgtacgc aaacttagga gcagaagtaa
    53761 cgattcttga aggctcacca caaattttac caacttacga aaaagatcta gtaaaattag
    53821 tagaagatga tttcaagaaa aaaggcgtaa cagtcgtaac gaatgcaatg gcgaaagaag
    53881 cagtcgataa cggtgacagc gtgacagtga aatatgcagt ggacgggaaa gaagaatccg
    53941 ttactgctga ttatgtgatg gtcactgtag gtcgtcgtcc gaatacagat gatatgggct
    54001 tagaacaagc gggtgtcgaa gtaggagaac gaggattgat cacggtggat aaacaaggac
    54061 gtacgaacgt acctaacatc tatgccatcg gtgatatcgt tccaggggcg gcattagcac
    54121 acaaagccag ttatgaagca aaaatcgctg ctgaagcaat ctctggcaaa aaagtagctg
    54181 tagattataa agcaatgccg gcagtagcct tcactgatcc tgaattggct tctgtaggta
    54241 tgacgatcaa agaagcaaaa gatgctggta tcgaagcaaa agcatataaa ttcccatttt
    54301 cagggaacgg tcgggcatta tcgcttggta aaacagaagg attcatccgt ctagttacga
    54361 cgatcgaaga taacgtgctt attggcgcgc aaatcggtgg agtcggcgca agtgatatgg
    54421 tctctgaatt agctttagca atcgaatcag gaatgaatgc tgaagatatc gccttgacga
    54481 ttcatcctca tccatctctt ggcgaaatcg tcatggatgc ttcggaatta gcattaggtt
    54541 taccaatcca tatttgattt gaataataca ggcgaaacgc aactgaaaaa tcagttgcgt
    54601 tttgtttttt agaattatca ataatattat tatgtaaact taaaaatatg acaacttctt
    54661 ggtttattac cacttcctat gctacactga agaggaatat gaaataggag agttgaagat
    54721 gcaagaatat cagtatccac ttgatttaga ttggacaaca gacgaaatgg tcattgtaat
    54781 gaagatgtgg gaagatctgg aacgtgcgaa tgaaaaaggg ataaacaatg aagctttttt
    54841 gaagacttat cagcagttta aaactgttgt caaatccatt ggtgaggaaa aacgattggg
    54901 acgggaattt gaaaaagctt ccggttattc tttatatcgt acagtgcagc aagcaaaaaa
    54961 aaatccaaac aaaactttga gaatgaacgg gtgagataaa tggaacagat gattgatcat
    55021 atcaagtcct ggcttgaaga agctggtgag aaaatcaaat cttctcaaca aacattaaca
    55081 atctcgcaaa aatcaaatcg aacggatcta gtaacaaatg tcgatcgaaa catccagaat
    55141 tttctaatcg aaaaaatcca ttcctttgat ccagaggcga aaatattggc agaagaagaa
    55201 gggtgtaatc aattatcgga tctcgatgga agagtcttta tcatcgatcc tattgatggc
    55261 accttgaatt ttgtgattga aggggaaaat ttttgtatta tgctcgctgt ttatgaatcc
    55321 ggcgttggaa agcttggttt catttatgac gtgatgagaa atgaactttt ttggggtgga
    55381 aaaggaatag gcgtgtataa aaatgatgag ccattgccta gaccagcaaa taaatcctta
    55441 tcggaaggct tacttggtgt gaatagttat cttttcggtc ataataaata tggaatccga
    55501 gaaattgcgg aaagcagcat ggggacacgt atgtacggat gcgctggtct tgaactagtg
    55561 gcaatcttga aaggaaacca cattgcttat atttcaaacc ttagcccttg ggattatgca
    55621 gcgggaaatg tcttattgga agagtttggt atggtaacaa gcggtctttt tggtactcct
    55681 ttgacatttg aaaaaaggga atactattta gcagggactt cttgtgcata tgcagaaatc
    55741 gcagaccttt tgaaaaaaga aggctgaaaa aaaggtagaa atcattcaca aaaatgataa
    55801 aaacgagcaa aagattaatt ttctcgaaaa aatgaaaaca ttttactata tttcatgaaa
    55861 aaagtctgct ataattagca gtcgagtaaa atttgccctg tcataaagaa ccaataaaaa
    55921 aggagttagt tacattgaat tatagaaacg atatccgtaa cgtcgcaatt attgcccacg
    55981 ttgaccacgg aaaaacaaca ttagttgatg aactattaaa acaatcagat acattagacg
    56041 cacatacgca attgcaagaa cgtgcgatgg actcaaatgc gctagaaaaa gaacgtggta
    56101 tcacgatttt agccaaaaac acagctgtag actataaagg tattcgcgta aacatcatgg
    56161 atactccagg acacgcggac ttcggtggcg aagtagaacg gatcatgaaa atggttgacg
    56221 gcgttgtgtt ggtcgtagat gcgtatgaag gaacaatgcc tcaaacacgt tttgtattga
    56281 aaaaagcact ggaacaacat atcacaccaa tcgttgtcgt aaacaagatt gacaaacctt
    56341 ctgctcgtcc tgagcatgtc gtagatgaag ttcttgaact attcatcgaa ttaggagcag
    56401 atgatgatca gttagacttt cctgtgattt atgcatctgc actaaatggt acgtcaagct
    56461 tgtcagatga tccagcagat caagaaccaa ccatggcacc tatttttgat acaattattg
    56521 aaaaaatccc tgctcctgtc gataacagcg acgaaccatt acaattccaa gtttcattac
    56581 ttgactacaa cgattatgta ggtcgtatcg gaatcggacg tgtatttaga ggaacaatca
    56641 aagtaggaga ccaagtagca ttaattaaat tagacggaac agttaagaaa ttccgtgtaa
    56701 cgaaattatt tggtttcttt ggactaaaac gtctagagat tcaagaagca aaagctggag
    56761 acttgattgc tgtctcaggt atggaagata tctttgtcgg tgaaacagtt actcctgtgg
    56821 atcatcaaga tgcgttacct atccttcaca ttgacgaacc aacattgcaa atgactttct
    56881 tagttaataa ttcaccattt gccggtcgag aaggtaaatt cgtaaccgct cgtaagattg
    56941 aagaacgttt gatggcagaa cttcaaacag atgtatcctt acgagttgaa ccaacaaact
    57001 ctccagatgc atggacagtt tctggtcgtg gagaattgca tttgtctatc ttgattgaaa
    57061 acatgcgccg tgagggatac gaattgcaag tatctcgtcc agaagttatt gaaaaagaaa
    57121 tcgacggtgt aaaatgcgaa ccgtttgaac gcgtgcaaat cgatacacca gaagaatata
    57181 tgggtagtgt tatcgagtcc ttgagcttac gtaaaggtga aatgcaagat atggtccata
    57241 caggaaacgg acaaatccgt ttgacattct taacacctgc ccgcggtttg attggttatt
    57301 caactgaatt cctttcaatg acacgtggtt acggaatcat gaaccatact tttgaccaat
    57361 atctgccaat gcttcctggt caaattggcg gacgtcacca aggggcgctt gtttcgatcg
    57421 atacaggaaa agcaacgact tattctatca tgagtatcga agaacgcggt acagtcttcg
    57481 tagaaccagg tacagaagta tatgaaggaa tgattatcgg ggaaaacagc cgtgataatg
    57541 atttgactgt caacatcacg aaagcaaaac aaatgacaaa cgtacggtct gctactaaag
    57601 accaaacatc tgtcatcaaa aaaccaaaac aattgacgtt ggaagaatct ttggaattct
    57661 tgaatgatga tgaatattgc gaagtaacac cagaaagcat tcgcttgaga aaacaaattt
    57721 tagagaaaaa tgctcgtgaa aaagcatcta aaaagaaaaa ataatctgta aaaggatgaa
    57781 gagagaaacc agcttgttat aaaaaatagc tggtttcttt ttttgtccaa ctcttttctg
    57841 tggagaagtc atctttgtca atggacataa attcagaaaa tacgccgaaa gacgtttaaa
    57901 agacttttaa aaaacttatt tctagagagt ttttgtttca taataagata atgtttcacg
    57961 aaaggagatc aacttgtcct tcgaagaaaa tcgaagtaaa atggcaagag aaaggagcgg
    58021 gaagatgaac gacttattaa aacaatactt tggctatgat gagtttagac ctggtcaaaa
    58081 agaaattatc cagaaagtca ttgatcagga aaatgtatta ggaatcatgc ctaccggaag
    58141 tgggaaatca atctgttacc agcttccagc tttactatta gatgggctta ctgtggttgt
    58201 gtccccttta atctctttga tgaaggatca ggtggacgcg gcgaatcaat taggtatacc
    58261 cgccactttt atcaatagct cgttagatgg ttacgaaaca gctagacgct tccaagaaat
    58321 cgatagacag cagtaccgtt tactttatat cgctcctgaa cgttttatca tgccggattt
    58381 tatccaagca atgaagcgat ggaacgtccg tatgatcgct attgacgagg cacattgtat
    58441 ctctcagtgg gggcatgatt tccgcccaag ttatttacag atggcgaacc agctagatca
    58501 actgccaaat cgtcctgtaa ttgttgcttt gacagctaca gcgactgtgc aagtagctgc
    58561 tgacataaaa agacttttaa aaattccaga aaacaaccat attcagactg gttttgaacg
    58621 agaaaatcta cgatttcaag taattaaaga tcaaaaaaaa gaacaatatc taatcgaata
    58681 tttaaagatc aacaaaaatc aatctggtat catttatgca gcaacgagaa aagaagtaga
    58741 ccgtctctat catttgctta aaaaattcga tttttccgtg ggtagatatc atggtggtct
    58801 aaatgagaat gagcgaacag aaatgcaaga agccttttta tacgatcgct tgcagctgat
    58861 tgtagcgaca aatgcatttg gtatgggtat aaataaaagc aatgtccgtt ttgttatcca
    58921 ttatcaaatt cctggctctc tagaagcata ctatcaggaa gctggtcggg cgggaagaga
    58981 tggtctttcc agtgaagcca tcctgctttt ctctcctcaa gatatccaag tccaaaagtt
    59041 ttttatccag cagtcccaaa gagaagaagg gcaaaagcaa aaagaatatg aaaaattaaa
    59101 agcgatgaca gaatacgtgt atattgagtc ctgtcttcag caatacatcc taaactattt
    59161 tggcgagaca agcagtccgt gcaaccgttg tggtaactgt ttagatgaca gagaaatagt
    59221 agaagtaacg acagaagcac agatggttct ttcttgctta aaacgtatgg gtgagaacta
    59281 tggtaaacaa atgctgatga aagtcttagc aggatcgaaa gaacaaaagc tgcaagcttt
    59341 aggttttgga catttatcta cctatggact gatgaaaaac caatcgcaaa aagaaacgat
    59401 gcagttaatt gaatatttga tatcgaatgg ctatcttctg accatcaatg gtgagtatcc
    59461 tgttttgaaa gtaacagaaa gagggataca agtattgaaa ggacaagaat ctgtttatcg
    59521 aaaggaaccc aagaaagtcc agcagctatc ggatgaagaa accgatacac tatttgaagt
    59581 gctgcgggaa ctgcgaacgg atctagcatc agaagcaggc gttcccccgt atgtcgtatt
    59641 ttcagattcc acattgaaag aaatgagccg aatacgtcca tcaagcagat tggagatgct
    59701 tcagataaaa ggagttggcc aaagcaagct tgataaatat ggcgaagcct ttttatctcg
    59761 gataaaaaat gcggactcta acgttctgga taagtagaaa gaacgaatac gtaaaaatga
    59821 ataaaaacaa ctggttaaat ctgttttttt tgctccttat gaaaatcaaa tatgttataa
    59881 ttaaggaatt agaatgggag gtaagcacag atggattctt tgtcgaaaaa acttgcgatc
    59941 caagtattaa atgatgaagc agaacggatc aaacgattaa tccgaaatca aaacaacagt
    60001 ctctgtatct cacaatgcaa agccttcgaa gaagtggtag atacacagat gtacggcttt
    60061 tcccggcaag tcggttttgc tgtccgtata ggagtaatcg gaaagaacga gggccagctg
    60121 atgttaagtg agctagaacg agaattgaat cagttatata gtgaggttta ccaagaaatt
    60181 tatgataaaa aagaagttgg caaggaggaa tagacttgcc taagaaagtg aaaaaaaggc
    60241 atctattaga ctatagcatc ttgattccct atttaatttt atgtgtattg ggattgatca
    60301 tggtatatag ttctacgtcg tatctattat tagaaaacgg acaaaatccg tccgcatcag
    60361 tcattaacca gagtattttt tgggtgctta gcttgattgc tatcgcacta ctttataaaa
    60421 tgaaaacaga tgttttgaaa aaccagcgtt tgatcatggc agccatcgca gttttgacga
    60481 ttttgctgct gattgttgtg ttttttggta aggagatcaa tggggcgaaa ggttggctgc
    60541 aaattgcagg attctcgatc caacctgccg aatacctaaa aatcatttct atctggtacc
    60601 tgtctctgac tttatctaaa agacaaaaca gtgtacaaaa agattttctt gggactgtga
    60661 aacgtccact tgcaatggtt atcggactta ctgcattagt cgctattctt cccgattttg
    60721 gtaatgcggc agttatcttt ttgattattt tggttctgct tttagccagc ggagtcaatt
    60781 atgtgtatac tttgattgta ggagtaggag gcttttgtct aagtactttt acgatctggc
    60841 tgatcaatat cacaaacgga aagatcttac ccggacgttt acagtatatc tataatcgat
    60901 ttgctattta ccaaaatcct ttctcagacg aattaaataa agggcatcaa ctagtcaacg
    60961 gctactatgc catgttcaac ggaggactat ttggcagggg attaggtaac agtatccaga
    61021 aaaaaggatt tttacaagaa gcacaaaccg attttattta tgcaatcgta gtggaagaac
    61081 tgggagtgat tatggggatt ttgatattag cgttgctatt ctttatgatt gtgcgcattg
    61141 tccttgtagg tatccgttca aaagatccgt tcaattcgct tttgtgcatt gggatcggcg
    61201 caatgttttt gatccaagta tttgtcaatc ttggaggaat tactggcgtt attcctttga
    61261 cagggatcac gttcccattt ttaagtcaag gaggctccag tttgctgatg ctttcgattt
    61321 gtgtcggctt tgtcctcaac atcagtgcgg atgaaaaacg caagagctta ggtttataat
    61381 aaaaaaagat gtaggagtgg ttcgatgaaa aaagttttag ttgctaacag gggtgaaatc
    61441 gctgtacgtg tatttcgtgc ttgtacagaa ttaggaatta aaactgttgg aatctatgca
    61501 gaagaagatg aatattccgt acaccgtttc aaagcggatg aagcatattt agtaggacaa
    61561 ggaaaaaaac cgattgatgc gtatctagat attgaaggga tcatttctat cgcgaaagaa
    61621 tgcggagctg acgctattca tccgggttac ggtcttttgt cggaaaactt gaattttgcg
    61681 caacggtgcg aagaagaagg catcattttt gttggaccca aactgcatca tttagatatt
    61741 tttggagata aaatcaaagc aaaagctgca gctatcgaag cggggattgc ttcgattcca
    61801 ggcacagatg gaccaatcgc ttctatagac gatgccttgg agtttgcgaa acaatacggt
    61861 tatccgatta tgatcaaggc agctctaggc ggcggcggac gcggcatgcg tgtagctcac
    61921 gatgaaaaga gtgcaagaga gggctacgaa cgtgcgaaaa gcgaagcaaa agcagccttt
    61981 ggtagtgatg aagtgtatgt tgaaaaatac atcgctaatc ctaagcatat cgaagtacag
    62041 attctcgggg atacgcatgg aaacgtcatc catctttttg aaagagattg ttctgttcag
    62101 cgccgtcacc aaaaagtagt agaagtagcg ccttgtgtct ctatgaatga acagcaacgt
    62161 caaaaaatct gccaggcagc tgtccaattg atgaaatatg tcgggtatgt caacgcaggt
    62221 acagtagaat ttcttgttga aggagatgat ttttacttta tcgaagtcaa ccctcgcgtc
    62281 caagtggaac atacgatcac agaaatgatt acagatattg atatcgtaac aacgcaactg
    62341 ttgattgctc aaggtttgga tctgcataaa gaaatcggat tgcctcagca agaaggaatt
    62401 aaattaaatg gttcagccat tcaatgccga atcactacag aagacccgtt gaataacttt
    62461 ttaccagaca cagggaaaat cgatacgtat cgttcacctg gtggttttgg cgtacgtctg
    62521 gatgtcggaa atgcttacgc gggctatgta gtgacaccgt attttgattc attgctagta
    62581 aaagtatgta cgcatggtgc tacctttgaa acagccattc agaaaatgga acgttgcttg
    62641 aaagaattta gaatccgtgg agtgaaaaca aacattccat ttatgctcaa tgtcatcaca
    62701 catcctgaat tccaatcagg aaatgcaaaa acaaccttca ttgatagtac agcaacatta
    62761 tttgaatttc ctcgactacg agaccgcgga aacaaaacga tgaaatatat cggtgaaatt
    62821 actgtgaatg gttttccagg tatcgaaagt ggcgaaaaac cattttatga agaaccacgt
    62881 atgcctaaag atctaatcac aagaactgat tacgtaacgg ctaagaatgt attagatgcg
    62941 aagggtgccg atgctttggt cgaatggatc aaaggacagg aaaatttgtt gttgacggat
    63001 acgacattta gagacgccca tcaaagtctg ctagcaactc gtgttcggac aaaggatttc
    63061 aagcagattg cacgtttgac tggagaaggt cttcctgaac tcttttcaag tgagatgtgg
    63121 gggggagcaa catttgatgt ggcttaccgc tttttgaatg aagatccttg gcaacggttg
    63181 agaaaaattc gttctttaat gcctaatacg ttgctgcaaa tgcttttcag aggttcaaat
    63241 gccgttgggt attctaatta tcctgacaat gtattggtgg aatttgtcaa agaagcagca
    63301 gcgcaaggaa tcgatgtatt tcgaatcttt gatagtttga actggactcc acaaatggaa
    63361 aaaagtatcc aagctgtacg ggatactggt aaaatcgcag aggcagctat ttgctatact
    63421 ggcgatatca acgatccaag tcgggcaaaa tacaatgttc agtattataa agacatggca
    63481 aaggaactag aacaactggg tgcgcatatc attgcaatca aagatatggc tgggctgctt
    63541 aaaccgcagg cagcttatcg tttgattagt gaattgaaag aaacgacaga tctgccaatc
    63601 catctgcata ctcatgatac cagcgggaat ggaatcatca cttattctgc tgcttcaaaa
    63661 gcaggagtag acatcgttga cgtggcaatg agtgcaatga gtgggaatac tagtcagcct
    63721 agcatgagca gcttgtatta tgcactggtc aatggtccac ggctgccaga aatcacgatt
    63781 gaaaatgcac agaaattaaa ccattattgg gaagatgtgc gcatgtatta caaaccgttt
    63841 gaaaacggct tgaatgctcc tgaaacagaa gtatatatgc atgaaatgcc aggaggacag
    63901 tattcgaatc tacagcaaca ggcaaaagct gtcggattag gtcatcgctg ggatgaaatc
    63961 aaacaaatgt atcatactgt caacttgatg tttggcgata tcgttaaagt aactccatca
    64021 tctaaagtag ttggagatat ggcactattt atggtccaaa atgatcttac agaagaagat
    64081 atttatgaaa aaggcgaaac actaagtttc cctgaatctg ttgtgacttt cttccaagga
    64141 gaactagggc aacccgttgg cggatttcct aaaaaattgc aaaagattat tcttaaaggc
    64201 agacctgcat tgaatgaacg tccaggtctt ttggctgaat cagtcgactt caacgaagta
    64261 aaaaaagaat tggcagaaaa aattggctat gaaccaaaac aagacgaagt attaagctat
    64321 ttgatgtatc cgcaagtctt tttggactat caaaaagcat ataatcaatt tggcgatgtt
    64381 acattactgg acacaccaac atttttccaa ggtatacgtt tgggtgaaac aatcaatgta
    64441 caaatcgaac gcggaaaaac gcttatcatt cgtttagatg aaatcggaga acccgatatc
    64501 gaagggaatc gcgtactgtt cttcaatttg aatggacaaa gacgtgaaat cactatcaaa
    64561 gataattcga tcatcagtac ggtccaaacc cgattgaaag ctgaaccgac aaaccgagaa
    64621 caaattggag caacgatgtc tggttctgtt ttggatgtat tagtgaaaaa aggcgataac
    64681 gttaaaaaag gtgatacgtt gatgattact gaagcgatga aaatggaaac ggccatcgaa
    64741 gcacgttttg acggagaagt cgcacatgta tatgtgtctt caggagacac tatttcgtct
    64801 ggtgaccttt tgattgaagt gactgagaaa taataagcga aaggtgggga aacatgaaaa
    64861 gactgggttt gttttggctg attctgctat tagttattgt ggcattctac atggaaccag
    64921 tcttgtttcc gccaaaactt gaagtgaagg aaaactctga tcaacaaata tcaaaccaaa
    64981 tgacaacgtg gaaataccag gagcttaaag caactggttt ttctacttat atcggaaaac
    65041 cgatcgatca gctggaaaag gattttggcc aagcctatga tcgattaact agcggatttg
    65101 gttttgaaac aagatattat gaaaataaag ctagtcatct cacatttgaa gcgaacatcc
    65161 aagacagcag agtcacaact gttaaagtac tgcaaacagg gcctgctgat atcgcgccgt
    65221 tttatttagg gatgacgatg caagatctta cgaaaatcac tactatctat actaatttca
    65281 cttttgagta ccatgataca gaagttggaa tcgaactaat ggaagaggat atgaattatc
    65341 gtccgctaat tgcttttgat aacgacactt ttgctatttt attttttgat caaacaacaa
    65401 acggcctatt ttcagttgtg tatttagata aagatagtct tttgaaacta ttgccatatc
    65461 aagtgtttgg tgaaggcctt ccacattatc aagcagataa atcggctgat tgggaatcaa
    65521 tcaatcgcgt taaagaacga aaaagtacga ctttattaaa tatgcttaga agagaagacg
    65581 aacttcccat atacaatcaa acacttgcct ttcaaaacca cagtcagctg ctactacatg
    65641 attttttgac aaggcctgag gaaatattgt cagaggaacg tatggctgat tggcaaaaat
    65701 cgctaggcag tgctcaaacg acagtgaaat tctcactgac gaatgatgaa ttggagaagt
    65761 taatcagtaa acaaaaatta gaaaatacta gtgggctatt tattcatccg gcgattgacg
    65821 ctactttttc gtttttatat tggtacagcg atccttacaa ccatgaccgt ttcatggacc
    65881 cgggaacgga ttctgtaggc attgcttttt ctaaagaaaa tatgttagtc ttattgcaag
    65941 aagaaaacgg agaaagtagt gatagcagtg atcgttaacg aagagctgtt tcaactggag
    66001 gatcaatgcc tccaagtcgc tgaaatgata aaaaagagta aaacgatggc ttcttatctt
    66061 gaacataaac agtcaatgga agaatctgca aaagtttgcc agttgaaaga agcgttcttg
    66121 tctaagaaag aagcatttga aaatgtagcg gcatatggtg agtatgcacc tgattatcgt
    66181 gaaaaaaaac gagctgttcg aaaagcaaaa agacagcttg atatggatga gcatgttgca
    66241 gctttccgat atagtgaaac acagctgcaa agtctgttgg atcaaattgt ccaagtactt
    66301 gccgaagaag tttctaatga aatcaaagta gatgcaggta gcccgttttt tgaaacgaaa
    66361 aaacactttg gatgcggagg taattgtcat gcaattagac aatagtgaat ttgaaataac
    66421 aaaacgacgc gggctgatcg tatgggtcta cagcttaaaa caattaaaga atctgaaaaa
    66481 gttcggcttg ttgcattatg tctcaagaag aatgaaatat gtcgtgcttt acttaaatga
    66541 agagacattg gaagaaacag aagaacgaat ccgtaaatta cattttgttc gaaacgtaga
    66601 acgttcttac cgaccggata tcgaaatgaa ttttgccgaa aaaataggaa acaaaacagt
    66661 attaaaagac gaaggtttcg aaattgaaga gctaaataca gaaatccgat tagctgaaca
    66721 tctttaaaaa tctcttatga gcagttttta aataaaaaat aacttggata acgatcgatt
    66781 tttgatcatt gtccaagtat ttttttggta tactagtacc agtaccattt ctaaggagtg
    66841 agtagatgcg gataatatca ggaaattatg gcgggcgccg tttaaaaagt ttagcagggg
    66901 cgaacaccag accaactaca gataaagtaa aagaatccat attcaatatg attggaccat
    66961 attttgatgg agagacagta ttggatctgt ttgcaggaag cggtggttta gctatcgaag
    67021 cagtatctag aggctgcagt catgcggtat gcgtagacaa aaattaccaa gcgctaaaaa
    67081 tcatcaaaga aaacatcgaa attacgaaag aaccagagaa atttaccaca ataaaacttg
    67141 atgcggacaa agcaatcgaa cggctagcag cggagaaaca acaattcgac tacttgtttc
    67201 ttgatccgcc atatgcaaaa caaaaaataa tcgatcaaat ccataaaatg gaagaactgg
    67261 gacttttttc taaaaccgca gttatcgtat gcgaaacgga taaaacagtc gaattgccag
    67321 aaactgtggc tggattccat caaataagaa aacagattta tggcataacg gccgtaacta
    67381 tctatagaaa agagggaaat caatgaaacg tgcattattc ccaggaagtt ttgatccttt
    67441 tacaaaaggt cacttagata cggtggaacg tgcggctaaa ttattcgatg aggtagtaat
    67501 tggtgttttt atcaatactt caaagaaaag tttgtttccc ccagaagaaa gaatgacatt
    67561 gatcacaaaa gctgtttctc atcttcctaa tgtaaaagtc atgcatcaag aaaatcaatt
    67621 gacggtagaa acagcaaaag agattggtgc agatgcgttg atcagaggca tacgaagtat
    67681 caaagacttt gaatatgaac gagagattgc tcagatgaat catcatctat atgatgaatt
    67741 ggaaactgtc ttcttattag caaaaccaga atatagtcac gtgagttcca gtattttaaa
    67801 agaagtactg cactttggcg gagatgtgag cagctactta ccgccagtga tcaacgaagc
    67861 tttagctgga aagcgtgaat caaatgaaag ctaaacaatg gttgaaaaca gtactgatta
    67921 tcatatttgg aatgttgatc attggatttg tagtagttcc cttgccgtat tatgtagaat
    67981 caccgggagc cacgatcgat ctgaaagaac taattacggt caatgataag gaagataaag
    68041 gttctgggtc tttctctctt acctctgttg gtatcaggca agctacaggt ttcttagctt
    68101 taaaagctaa aatgacccct tttgaagaat tgatcagtga agaagaattg actggtgggg
    68161 cgacaaatga agaatataat caaatgcaac agtattatat ggattcttcc caaaatgcag
    68221 caatcgaaca agcgttaaaa cttgcagaca agccttataa aatggaattc aaaggcgtat
    68281 atgtcatgag tgtagagaaa acatcgaatt tctttggcaa gctttcagtt ggagatacag
    68341 taacaaaagt agacgggaaa agctttcaaa gtacagaagc atttatggat tatgtaaaaa
    68401 gccaaaaagt tggacaaaca atcgaaatca gttatttgca taatggtgaa gaaaaaacag
    68461 cttctggtga tctgatcgag ttaccaacag ataaaaaggc aggaataggt atcacgctta
    68521 ctgaccatac cgagatcgaa tcagatacag atgttcgtat taattctggc tctatcggtg
    68581 ggccatcagc gggattgatg ttcactttag aaatctatga acaagtcact ggtaaaaact
    68641 tgcgacatgg taagcaaatt gcaggaactg gaacgatcaa cagtgaagga gaagttggcc
    68701 gaatcggagg aatcgataaa aaagtggcaa gtgcggataa agctggcgta gagattttct
    68761 ttgcaccaga tgatacgatt tcagacgatg ttaagaaaga atatcctgaa ataaaaacca
    68821 actacgaaga agcaaaagct gccgctaaaa aattgaattc ctcaatgaaa atcgtaccag
    68881 taaaaacagt acaagaagcg cttgactatc tagagaaaat gaaatgaaac taaataatta
    68941 aataggggtt gtgacacgag ttctgtcaca acccctcttt ttcgtcaaaa atcttcacga
    69001 taaaaataaa aacaaaagaa aaaattgacg taattagatg ttgaagggac gattttgcca
    69061 gagctcactg tttcttcaat tttaaatgaa aggagtaatg aatgattttt actggtcatt
    69121 ctttgaaaaa agaacaatga tagaaaaaat cagcaagaaa atactagctt cacccaaatt
    69181 gatcatttgg atgtctatga tagttcttat cttttgtatc gcaagtggtt ttttattatc
    69241 aaatttgcta ttttatagag aaacagaaga gactgctgca aaagaaatac tttcttcgtt
    69301 tagttccagt ggagaagatc catcagctga aggaaacgat gtagaagagg atgctcgtcc
    69361 aaaggtcatg tacaccgata tcaaaggaag tgtgaaggag cctggtatct attccttttc
    69421 ttcagaagaa cgggtctatg atgtactaaa gcgtgcaggt ggattattag aagaagcgga
    69481 tagcgatcga atcaactttt ctgcgaagat agaagatcag caagtcctat atatccctgc
    69541 tgtaggtgaa gagctgccag aacatcttga tcagttggca tcctcggaag gaaaacagcc
    69601 tacagcagat tccgagcctc ccaaaataaa tatcaataca gccactccat cagaacttca
    69661 gcagattcct gggatcggat cagtcaaagc gcaagaaatc attcgattta gagaagaaaa
    69721 cggatccttc cagaaagtgg aagacctgca ggagatttct ggtatcggtg aaaagacagt
    69781 tgaaaaacta aaaaacttcg ttacaattaa atagaatgat tcaaaatcga ctggcacgag
    69841 caataaatgg acgctaatgg atactaagaa taatccaatt ctaagagctt ggcaggacga
    69901 ggaggtagta tttttggata ggaaatagct tgattgttcg caaagaaaca ttaaaaaaac
    69961 caatgctgaa tgtctatctc caaaataaaa tatctgggat tcatattatg aatacggctg
    70021 tcagcggcaa taacagtcaa gctctacgag aacgctttgc caaagatgta cttagctata
    70081 cagcagataa agtatttatt ctcattggga cgaatgatct agcagagcat aaacaacttt
    70141 ctaaagaaac gtaccaaaaa atctgcagtg gatgatagtt cagctgaaaa aagactatag
    70201 ttctgatcat gctttcttta tcacgccacc agcagtcgat gagacaaaac aaaaatatcg
    70261 gaataacaag ttgatcaaag aatatatctc tgttctcaca gaagtagcaa aacgccatga
    70321 ttgtgaagta tcgattttta tacggaattt cttgaacaaa tggattcgaa gaatttttat
    70381 aatgggctaa tggatgacgg acttcatttc ggcagtgaag ggtaggatct tttgtcagac
    70441 aggatgattc gttatattta aagaatattt tgtttaagca atgagtactt tttaatcaac
    70501 ttgaaaaaac ggaggaatat aaaacatgtt agaaaaatct tcaaaagcat gcgatacgaa
    70561 taaagaaaaa gttactgata agtcacataa tgaacgcatt ccttgggacc agtactttat
    70621 ggctcaggca gtactgttat ctttacgtag tacgtgtaca cgtttagaag taggagcgac
    70681 actcgttcga gaaaaaagga tcattgctgg cggctataat ggagcagtca gtggagatgt
    70741 ccactgcata gatgaagggt gttacgtcgt ggatggacac tgcttgcgaa cgatccatgc
    70801 agagatgaat gcactcctac agtgtgcaaa acttggtatc ccgacggaag gttcagaaat
    70861 ctatgtcaca cattttccat gtttggcctg tacaaaggct ttgttgcaag ctggtgtgaa
    70921 agggatcaat tatctccatg actatcgaaa tgatccttat gcccaagcgt tgatcgaaca
    70981 attaggtgta tctgtacacc aagttgcttt agactcacag catttttcaa aacttcaaaa
    71041 tgaattaaca ggaaaacatg tctagcgttt tagaaaggca tgttcgaaat cggttgattt
    71101 ttccggcaat tacgtcgatg attatctgtt tgttgtatta cactgacaat tggggttatc
    71161 gtttgcttct tgccggaatt tttttatggt tttcgctaaa agaaggacgt tttatcgcgt
    71221 acttgtgtat tttgtgtagc ataacggtat ccatttctta taggctacaa gagtattcat
    71281 ttttagagga aaaaacaaca tctgcgcaaa ttactgtatt tacagatacg attcgtgcaa
    71341 atggtaattt tttgacaatg gaaggaaggt tatcagagaa taaacaaaag ctccagcttt
    71401 cttatcaagt aaacacagaa gcagaattga aaacatggct taacttccga ggaaagggaa
    71461 tagaattgac tgctgaaggc gagttcatct ctccagcaaa aaagcggaat ctttataatt
    71521 ttgaccaaga agcttacttc aggaaccata gaatttctgg acggttccaa attcaaaaga
    71581 ttgaacagat caccatgcaa aatcactggc gaaactggct tcagagaaaa agaggatctt
    71641 ttatctcctt tgtccaatct gaatttccgc aaaaaacaag tgtatatatt aattctttat
    71701 tatggggcta taaagatgca ggattcagag aaccagaaga aatttttagg gagagtggtt
    71761 tgctgcatct gttcagtcta tcgggtctac acatccaatt ttatttaggg tggctgtatt
    71821 atttgtttcg acgtatcgga tggccactac acgtcagtat tattccgttg agtctactaa
    71881 ctatctgtta tgtttctatt gcaggaagtt ctatttctgt tttgcgtgct agtttgctgt
    71941 ttttattgcg attgctagtc aaattgctga ataagcatta ttccaccatg gacttatttg
    72001 cagttgtctt ttggcttctt ttactatttg atcctcgatt tctttttcat gcgggtggac
    72061 agcttagttt tttcatgagt tttttgttta tcctgatttc ttttgatgga gattggttga
    72121 aaaaaacggc ctctcacgtc ctgtttacta tgatcacgat gccgttgatt gtatggcatt
    72181 tttatgagtg gcctatttta ggtagtcttt ttacattact gattgcaccg gtattcaagt
    72241 ttctttttct tcctggtgtg ttatttctca cactcttcaa tcgctggctg ccggaaactt
    72301 tacttttgct gatagaagag ggattaatct tatttgagcg aataatcgat ttttcttcag
    72361 ggtcgtctct cattatagga cgtttgcccc ttgtgctatc tgggatgtat attttcggat
    72421 tactgatttg tatggatagg ttgaaagaac aacctgtgaa acacttgttt tatgttggtc
    72481 ttttttcttt tatttttttg actttgccat ttttacgctt ttcttcaacg atcgctttta
    72541 tcgatgtcgg tcaaggagac agtatctttt tgcaaagtcc ttttcgaaaa gaaacgattt
    72601 tgatcgatac tggaggtcag ttacactttg aaagagaaaa gtgggcgcaa ggtatgatac
    72661 gtcctcctgc tgaaagaaat atcgttccat atctaaaagg tcaggggata agtgtaatcg
    72721 ataaactcat cttgacacac gacgatacgg atcatgtggg agaattaccc acgttatctc
    72781 agcattttac tatcaaaaaa atttatatcg gttggggcgc tggacgaaat gagccattac
    72841 aaaggcattt atctgaagct caaaaaaatg gtactgagat cattgaaatc aaacatgggg
    72901 acaggatcag cggttattat gatctttatg tattgactcc ttttgaaaag ggagagggaa
    72961 gaaatgaaga ttcgattggt ctatggatgg aatataacaa taggcgcttt ttatttttag
    73021 gagatttgaa tcaagcaatg gaaaaacaat tgcttttgat ttatcctaat ctgaaagccg
    73081 atgtagtcaa attaggtcat cacggtagcc gtacgtcaag taacacagat tttctttccc
    73141 atattgaagc aaaacatggg attatatcct gcggcgtaaa taatcgttat ggtcacccgc
    73201 acagcgaagt attggagaca ctagaagaaa atcagataaa aacatggcga acggatcaac
    73261 aaggaatgat cacctatcgc tggcatccag tttttcatcc tcatgggcta gtggaaacaa
    73321 tgatagacta gtaattctca tctgctcatg gtaggataat ttttgaaagt ggtgagtaat
    73381 ttgagtatac aagcagagtt acaaaaaata cgtaaagaaa aacttgcatc ttgttacttg
    73441 attttaggaa cagaaaagtt tctccaagat caagttcgga cagaaattgt caaaaaaatc
    73501 caattagagg gcgaagatga cttaaatttt ttaagttttg acatggaaaa tgcaagttta
    73561 gatgaggtag tagctgaagc agagacgttg ccgtttttcg gagatcaacg gttagtattc
    73621 gtagagaatc cttatttttt aacaggtgaa aaagtaaaca atggaatcga acaaaatact
    73681 gatttattga cggcttattt gaaagaacca ttagaatcga ctgtattagt attctttgcg
    73741 ccatatgaaa agttggatga acggaaaaaa gtaacgaaac aattgaaaaa aacagcaatt
    73801 atcatcaatg tccaacagtt gaatgaaaaa gaagtacgtc aatatctgat gaatacctta
    73861 gagaattcct caatcaaaat ggaacgaagt gcaattgatt tattcttgag gttgaccgac
    73921 ttagatttat ccaaaatgat gagagagtta cagaaattgg tcctttatgg acaaaatcaa
    73981 cagcaaatca cggtaagaga agtagaagaa ttagttccta aaacgttaga acataatatt
    74041 tttgacatga cccaatatat tttaaaaggg aagacggaac aagctttgcg tttatacgaa
    74101 gatttagtta tgcaagggga agaaacaatc aaaattaatg cgattttgct ttctcaatta
    74161 cgtttatttt tacagacaaa atttcttgta aagattggtt atcagcaggc aaatattgct
    74221 gaaacattga aaatccatcc atatcgtgta aaacttgcca tgcaggaagt aaaaaaattc
    74281 gatgaacatt tgctggtaca gttgtttgat caattagttg agatggatta tcaaatcaaa
    74341 acgggacaaa tcgaaaaaga attaagtttt caactattcg ttttgcgcac agggcagttg
    74401 ttaagaaatc gataagaaga aagacgaggg caaagcatgc gggaaggtcg tttgtttttg
    74461 tccttttttt gacaaataaa aaaatcggcc gaagccgatt cttttattta gcgatttttt
    74521 tgcttaaacg agatttgtcg cggttagctt tgtttttgtg gattagacct tttgattcag
    74581 ccatatcaat agctttaaca gcttctttat ataacgcatc tacgttgtca gcacctgaag
    74641 ctacagcatc ttcaaatttt ttgattgcag tacgcatagc gcttgtttga gatgaatttt
    74701 tcacattagc gttttcgctt gtacgaacac gtttgattgc agattcaatg tttggcatta
    74761 gtttcacctc cgataactta agttcttacg cgtgttaccg cataattcaa cattcattat
    74821 tatacctaaa ggcagcgtgc tttgcaatat gaacatgaca agtttttttc cttaacagtc
    74881 ttttattagg attttttttc agtaaaaacg gatagtacaa gctgaaaaaa atcaaaaaga
    74941 cgctattgtg ggaggcaata gcgtcaaacc aaactagatt tttatttatc tatttaaaca
    75001 aagtttgaat gggaatgttt ggtactaagt tattatataa caaagagaac cgtttgcaaa
    75061 gttttttttg aaaagggata cgattccctg ttgttctttg tttttctatt aagaaactgc
    75121 agttatttta taaccagtat cctttgatca gtaaaggcta ttctggtgta ccgattattt
    75181 taatgtattc atcgatattt tttatcaaca ttttttttat gaccaactgg ttccgacggt
    75241 tccatttgcc acttacttcc agcacatatt tatttagggg caaacgtaag atatattttg
    75301 ccaaatagcg atctttaagc aggccattga tcacacggtc ctcaagctgc aagttgatcc
    75361 cagtcagcat cattggctgt tcttgtaaaa cgtacgtatc aattaaaaga ccttcaaaag
    75421 tttccacaag cgtcttcctt tcttttcaaa gtaacctgaa aacaggcact aattcttcca
    75481 ttttgatatt cgttgaagtg gatgcgatca ttctttcttt tgtccactgt aatctagtat
    75541 aacaaaaaag agcagaagag ccaaaaagaa aaaagagcat cgaattctat aggaagtctc
    75601 attggcacgg cctaaattat tacaaaacat tgattaaaaa aagcccctgc atcgcaggag
    75661 catcccctat atcgttttaa taggtcaatc aaaaagcagt atcaaacaca ttttgtatca
    75721 aatcaggaaa taatagataa gctaagccca gaatcaaaag taccgctaga agaaaccagc
    75781 cgattgttcc tatgaaatac tttaattttt cgaacattgt atcacctcgt tgtcagtata
    75841 tcacggatca gtaaattggg ggtgttaaga atctcctaaa atagacaaaa tttgttctat
    75901 tttcctaaag caaatacttg taagatacag taagtgatac cagaagcaat gaccggtccg
    75961 gcggcgattc ctcttaagaa gacaacacct aaaatcgtgc cgaaaacaag tgcaacagtg
    76021 atttgtggat cagaagcaat caatcctaca cccttggaag ataagacagc cactagccca
    76081 ccgcaaaaaa cagctaccca gccaataggg gatttaaagg cttctaatag atcacgtaga
    76141 ccgatttctc ctgttgcgat agggattaga atagccgctg tgatgatcgt caccccaata
    76201 ttgattcctt tgccttgaag ccaagggaaa aaggaacggg aggctggtat gagcttcata
    76261 atcaaaacaa aagctgccgc tatcatcaga ctttgatttt tagctaaaat tgctacaagt
    76321 aaaataattc ctaaaaaaag ccaactttcc atgaaatata ttcctcactt tcttcgaaac
    76381 aatcataaca tgtttttaga aagttacaaa agtaggaatt catccttcaa gacctattca
    76441 taagctgttt ttttcataaa ataaacaaaa tcataaaaaa acactttgct tatttgacag
    76501 aagacagccg cgctttgtat agtggacaag acttacattt ttttgaagaa tcagaaagat
    76561 caggtaaagg tcttagtaaa cgcaagccat tcagaataac caatattgtg cttccttcgt
    76621 gtcctacaac tccaagaggt agatttaaaa attgcaggaa gttagaagca atcaacaaga
    76681 gaataacaag aatcgaaaaa ataatatttt gtttgatgat ccgccgcatt tttaaagata
    76741 gccgatgact gtagacaaat ttatttaagt cgcttttcat taagacgatg tctgcgatat
    76801 ccatagcggc atctgtccct tgccccatag cgacacctaa agaagcggct gctaaagcag
    76861 gcgcatcatt gactccatca ccaaccatcg catttacttg gtaaagttca ttctcttttt
    76921 tgactagttg tgttttttct tccggagtac agcttgcata gtaatgttca atccctactt
    76981 gttcagcaat ggcctttgct gtgccagaat ggtcgccggt tatcatactt gtatggatgt
    77041 tttgggattt aaagtaggaa attacttgtt gggtatttgc ttttggaata tctagcagac
    77101 ctaaaacagc aattaactgc tgatctttag aaagataaat cacggttttg ccttgatttt
    77161 ccaaacgttc gatcatttcc gcaatctctt tgctgattat catcgtctca ggatcatatg
    77221 cgtgcttgcc aactttccat tgcgcaccta aataggtagt ttgtaatcca aaaccagtaa
    77281 tttcttctat tggtaagtat tggatttctt caggtattgt gcaatcgaaa tattgcaaaa
    77341 tcgcatgagc aagtggatgc gtcgtttttt gttccatagc aacaagaagt tgttgtgcca
    77401 aatccttttc atcaaagaaa aagctgtcag ttacaactgg ttttccttta gtgagcgttc
    77461 ctgttttatc aaaggcaatc gcttttaatt cagaaagctt ttctaaatgg ataccgcctt
    77521 tgattaaaat cccatttttt gcagaatttg aaagagcagc tagagaagct ggtgtagcag
    77581 atgcgacaag cgcacaagga gaagccacaa ctaataatac cattcctcga taaaagcttt
    77641 catcccaact ccaacctagg aaaaagtggg ggagaaggat cattagagga acagcaagca
    77701 aaatcaattt tacataatta ttttcgattc gttcgataaa tgaagctgtt tgagttggcg
    77761 tattctgcgc ttcttctact aattggataa tcttagcaaa gacagtttcg gaactcgttt
    77821 ttgttactcg aatcgtgatg ggatttcctt gattcagcgt tcctgcatac atctcatcgc
    77881 ctaaatattt ttctacaggc acagattcac cgcttaatgc agactcatcg atcgttgcct
    77941 gtgtgctttc taaaaagcca tctatcggaa cagctgctcc tttagcgaca aataccaagt
    78001 tatccacctt tagttgagaa acatctactt cttgcatttg tccattttct tttaataatt
    78061 gggctttttg tggttgaaga ttcatcaaag cagtaatttc tttttgactt ttattagttg
    78121 tgtattcctc taatgcaccg cttaaacaaa agatgaatgt caacattgct ccttcaaagt
    78181 aattaccgat caaacaggca ccaatagcag ctaaagccat caataaatcc acattcagtg
    78241 tccgatcatt ccacaattct ttcaagcctt ccgatgtttg tttatatcca ccgatgataa
    78301 tggcactgat ataaaaaagt ggggtgatgg taagagagaa tcggtcgaag ataaaaccga
    78361 agatcataaa taaaaggcag ccgattgttg ctgcaaaagc ttttttctga ttagtcatcg
    78421 ttattagacc tccttgtcaa aacaaggata gcataataaa aatgtaattg ataataataa
    78481 cagctaaatg agaacgataa tggttatcat ttggtgatta agcttgatgt tgtttttatt
    78541 atttataatg attattatta aagaaaataa aaaaccagaa catttctcaa ataagagaag
    78601 tgttctggtt ttttctacat tgcaataaac caataaccaa tcaaaatgat accaaggata
    78661 atacgatacc agccaaaagc ggtaaagtcg tttttcttca aatagcttag taagaatttg
    78721 atagctaaga cggaaaccac aaaagatacg aatgttccta caagaagaac aacagtttct
    78781 tccattttga atgaattccc ttcagctaaa tatttgacga tcttcaagcc gcttgcgcca
    78841 aacatgacag gaatccctaa gaaaaatgaa aactctgtag cgacaaaacg cgaagcacca
    78901 atcaagatag atcctaggat cgttgcgcca gaacgggatg ttccgggaac aagtgccaag
    78961 acttggaata acccaacaag aagagctgct tggtacgtaa agtcattcag agaagtccaa
    79021 cgaggtgttt tgtttttgtt gcgtttttcg acaataataa aggcaatacc gtaaacgatc
    79081 aaaacaatgg caactggtaa gaacttgtgg aaatttgctt ctaaccaatc atccaaaggc
    79141 aaaccaatga tgatggctgg tatgcaagca acgatcactt taaaccaaag tgtccacgta
    79201 tctttttttt ctaccgttga tttacttgga gaaaatgggt ttaacttgtg aaaataaaga
    79261 acgacgacag ccaagatcgc ccctaattgg ataacgacat tgaacatcga cataaattgt
    79321 gaactggcat ccagtttgat gaattcatct gctaggatca aatgaccagt actactgatg
    79381 ggcagccatt ccgttattcc ttcaataata ccgagaatca ctgctttgat aatatttgca
    79441 aaaaacataa attccttcct tcccaattaa gatacatctt atagtatacc gttatttttt
    79501 gagaaaccca atctgttttt taatattaag aaaaacttga gaaataaagc agataagtac
    79561 acgaagagaa aaaagaagac aaagccctcg ttcagacttt gccttttatg attctctggt
    79621 aaaaattaaa atatccccat tatagaaacg agtcgcgact atgtgttttc ccgttatcga
    79681 tataagacgt atcattgatg ccgtggatag aaaatcgacc gtttgcataa gtgacctttg
    79741 ttacgctgcc atttaaaagc ccagcacgaa caggctgttt aggatcgatc agattaagca
    79801 aaaaggcgat cgtcaaacca tgagatacga ctaaaacatt tccaccacca tttttttcgc
    79861 agtgatgggc gatatcttca aatcctgtcc atacacggtc tcgcaatgtt tcataagttt
    79921 ccgcccaccc agcggtatct aagttgtata cagcattagc aatttgttca aaagaaactt
    79981 ctgtggtaaa catttcatca tatgttttaa aattcaatac acgagggatc acaccccaca
    80041 tttccatgtc atatccacct tcaagagacc cgaaacacca ttctctaatc cttttttcaa
    80101 taaaataagg aatttctttt cccttagaat gttctcctag aataatacgg gcagtttcga
    80161 ttgcacgacc actgtcacta gaataagcaa atgaaaagtc gacatttttt aacccgcgac
    80221 ctaaataatg aatgccttct tgtccttgct ttgtgagggg tgtatcacac cagccttgta
    80281 ctcgttcaat tgtattgaac atcgtttttc cgtgtcgtac gatataaagt gtagtttctg
    80341 tcattttatt ctcctcctaa gtcgttttaa cgattgaaaa aaggatttgt ccttttttcc
    80401 tgttcaatcg tcgttgcatc gccatgtcct ggatagactg caaattcatc aggtaataca
    80461 aacagctggg tccgaatact atgaagtaat tgttctaaat tccctgtata cagatcggtt
    80521 ctaccgatac ttcctctaaa cagggcgtcg ccagaaacga caaaatcatc aaaaataaag
    80581 ctgacgcttc ctatagaatg ccctggggta gggacgacgc gaaaagacat gtttcctaaa
    80641 gtataagttt tcatttcaaa ttcatattct gcaggcgaga caataacatc agcaatatca
    80701 tcatgtctac cgagtccaga aagattcaaa atcggatcac ctagccattt ttgctctagt
    80761 gggctgacat aaactgggat ttgataatac gcgcgtaaag gttccactgc accgatatga
    80821 tcgtagtgag tatgggtaag caaaatcgct accggctgtt ggtttgtttt tttgatttct
    80881 tgtttgattc gttccgcatc ttctccaggg tcaatgatca aaagtgcttc ttcgttgtag
    80941 accaaataac agttttcttc aatcattcct gtttttaatc tttcgatatg catgtgcatt
    81001 cctccttgat agccaaaact tctttcatcc attagtataa cgcattcatt gctaggaaac
    81061 aaaaacaggt acttatttct ctttacttat ctgaattatc agactatatg aaaagtattt
    81121 ggatattcta aagagaagga aagtaagttt tcatagcatt ttaatatgtc cttgttacat
    81181 tagattcatc caaaaatcca aattctatat cctatatccc tgcctaatca gcagggtgtt
    81241 tttttatgtt tggaaagaga gctctttttg ctaaactagg ttttttcagg taatcttaga
    81301 gagaaagagg tggtggaaat gatcaaacag tcaaaaatat ctgtgcggca cttagtagaa
    81361 tttattttgc gacgtggaag tatcgacaat cgtaaaaaaa gtaaccatac tgcattagaa
    81421 ggagcgagaa tccaccgcaa gctccaaaaa gatgcgggag aaaactatga aaaagaagtc
    81481 tttttaaaaa caacagttca attagatgaa tattacctga tcgttgaagg ccgtgcagac
    81541 ggtattttca aaagagacgg tatttattat atagatgaaa tcaaaacctc cgaaccacga
    81601 tttgaagatt tagaaccaga acaagtggaa cttttctttc atcaagcaag ggtgtatgcc
    81661 tatatctata gtcaagaaca tgatttacaa gaaataaatc tgcaattgac ttacttccag
    81721 acggcagaag aagtgatcac gagaaaggtt gaacatcaga caagagagca gttagaaact
    81781 ttttttaaaa agctgactga agattataaa gaatggcttg tttttcaaga aaactggcgc
    81841 actgtacgca atgcctcttt gatggctttg aaattccccc atgatgagta taggaaaggt
    81901 cagcgtgaac tagcggtagc tgcttataaa acgatccgta cgaaacagaa attgtttgta
    81961 gaagcaccta ccggaacagg aaaaacaatc tctacgttat tccccgcttt gaaagctgta
    82021 ggggaagaag agggagaaaa gatcttttat ctaacggcta aaacaataac tagacaagta
    82081 gctgaagatg ctatgacagc attaaaagat acaggagctg aagtaaaaag tgtcactttg
    82141 acggcgaaag acaagatctg ctttttagat gagacgacct gtaatccaga tcagtgccca
    82201 tatgccaatg gttattacaa tcggattaat gaaggtttat gggatctttt gaatcatgag
    82261 aatcagatca ctcgtgaagt catcgaaact tatgcaagaa agcatacact ttgtcctttc
    82321 gaactatcat tagatgtcag tatctggtgt gacgtaatca ttggggatta caattattta
    82381 tttgatccaa ctgtttattt aagacgtttt tttgaagatg aaaaaaacga ggactatctg
    82441 tttttgattg acgaagcaca taatttggtg aatcgttccc gagagatgta ttcggcagaa
    82501 ctatcttatg aaaaaacgaa acgaagtaag gaagctatac caaaggaatt caaaaaactt
    82561 catcgccgtt tcaacaaact attaaaagaa tttgattcga tccgagaaat agcaaaagaa
    82621 gatcattggg attatcatca tcaaaaagca ccggctgaat ctttagtaaa ggcaggttat
    82681 caattaagcg aaaaaattaa agaatggctg gcagaatttc ctgagcaccc atcacaagaa
    82741 caattgcttc cttattattt tgatttgctt cattttttga aggtcagtga attttatgat
    82801 gatcactacg aaacaacagt cgaaaaaacg tatcgcgatc tgatagtaaa agagttttgt
    82861 attgatccaa gcttgtttct tgagcagtcg ttggataaag gaaaaagcag tattcttttt
    82921 tctgctagtt tttcaccact ctcttattat caggaaacac tggggggaca aaaaagtctg
    82981 gcttacaaac tgccgagtcc tttcccagaa ggaaaccagc aagttttcat cgcaaattac
    83041 ctagagacga cttatcgaaa aagggaacac agtctggcaa aattagtaga gacgattcat
    83101 gcttttgctg atggaaaggt cgggaattat ttcgttttct ttccttctta tcaatattta
    83161 gatcttgcgg tggaagcatt cagacagcaa tatcccgaca gcaatgtgct aatccaagaa
    83221 acggacatga atgaggaaga acgcgaacga tttttaacaa aatttcaaac aaatcccgct
    83281 gaaacattga ttggattttg cgtacttggc gggatttttt cagaaggaat cgatttgaga
    83341 ggggaccgat tgatcggcag tatgatcgta ggtgtaggtt tgccgcagat gaatcatgag
    83401 caggaactga tcaaatcata ttatgatgag aaggaacacc ttggatttgc ttacgcttat
    83461 cagcttcctg gaatgaacaa agtcttgcaa gctggtggtc gagtgattcg tggcatggcg
    83521 gatcaaggaa tcgttgtatt ggcagatcaa cgattttcta gtttttctta tcgacgttta
    83581 ttcccacaac attggcaaac agcaagagaa gtacgaagtg ttggggaatt ggaagaagga
    83641 atacagcgtt tttggttatc aaaagaagca gtaaatcaaa aaaacgagga atgacagacg
    83701 tcagcctcgt tttttgatta acgatagtta gtgaattgca actcgattgg taagtcaagt
    83761 ttacgtaata aagcaatcac agcttgtaga tcatcacggt tcttacctgt cactctaatc
    83821 tgatcttctt ggatttgtgc ttttactttc agtttttcat ttttgatagc tgtagtgatt
    83881 ttcttcgcgt tttcacgatc gattccgttg accaaatcgg cagtttgtcg agctttgcca
    83941 cctagagcgt gttctgattc ggaaaaatgg atatttttga ttggcacatt tcttttgatc
    84001 agcttaccaa ataatacatc tttgatctgt tcgattttga aatcatcatc gctcaataca
    84061 actagattgc tgttctctaa tttgatttct actgtagaat ccttgaagtc aaagcgattg
    84121 gacaattctt ttgttgtttg ctgaatcgca ttttttactt cttctgtatt gacttctgat
    84181 acaatgtcga agctagcatc ttttgccata ggaaccctcc tgatatacat ataaatattt
    84241 accatacatc tatcttatca taggattagg gcagattctt acctgtttgc taaaattctt
    84301 ctcatttgag ttcgaagtat tttattttaa tagataaact gttattctta catttggaat
    84361 accaaagtag gggagaggtg cttgtgagta aaattatgac tgcgtatcat aaaaatgaaa
    84421 gattaaagaa aatagtggtt attttgataa ccggattgac cgctgcggtc gctttaaatt
    84481 attttttgat tccagccaat gtcttttctg cagggatgaa tgggatcgct caaatcattt
    84541 cctcgctttt atatgaatgg tttcatatca agatagacac aggaacattt atttttcttt
    84601 tgaatatccc tgtgtttgta ttagggtttc tcaaagtagg aaaacgagca acgatcctta
    84661 gttttgtgaa tgttgtatgt gtatcggtga tgacgatgct gcttccagtc ggacagatca
    84721 ctgataatat tttgatgaat gccctagtag gcggtgtttt attaggtctt ggcgtaggat
    84781 tatctttaaa aatgggcttt acaactggtg ggatggatat catctctctt gtactttcac
    84841 aaacaactgg aaaaacagtc ggaaactaca tgctgattct aaatggtatc attgtagtca
    84901 tcgcaggctt cttctttaac tgggaaagtg ccttgtatac gattatttcc atttatgcaa
    84961 tgactcaagt aattgatgcc atccacacga gtcatcaaaa agtaacagca atgatcgtta
    85021 ccgtcaagcc agaagaggta acagaagaac tagcaaaacg gatggtacgt ggaatgacat
    85081 tactgccgtc tgtcggtggc tattcaaaaa aacaagggaa catgatcatg atggtcatca
    85141 cccgctacga aatgtatgat cttgatcaga tcgttcatga aatcgacgac gatgcattta
    85201 tcaatatttt accaactcat tcagtatttg gccggtttgc aaatgaaaac gaacaaaaaa
    85261 tgtatcgaac aactggcgta ttgccagaaa taaaaaatcg ttcacaaaaa aaatgaggaa
    85321 tcaggtttat tttagaataa atttaatcta ttacatctaa taagaacatt tttataaatt
    85381 taaacgagaa tcttacttct aaattctcat ttacaagcag ctaaaaaaag agtatgataa
    85441 ataaaaatcg tgttaaggag tgtcgtacga tgagcgaagc aaaccaagaa cggtctggac
    85501 aagaaataga actaatcaaa gaacgtattc ttgctgcctt ggaaatggtg attgatcctg
    85561 aattaggaat cgacatcgtc aatttaggct tgatttatga tatagaattc aatcctgaaa
    85621 atggagaaac agtcattaag atgactctaa caactatggg ctgtccatta gcagatattt
    85681 taacagaatc tatccatgac gcattaaaag aagtgccaga agtaacgaaa gcagaagtca
    85741 aactagtctg gtatccagca tggacgacag ataagatgag ccgttatgca cggattgcgt
    85801 tggggatacg atagaaatct tggctgacta gctatgagaa gcaaatagga aaaacaaaaa
    85861 agccttttat cctttctaga agaaaagaga taaaaggttt ctttgtttat aaaattttgt
    85921 ctgctatatt ataagttaat taaataaatg tgttctattg ataaaggaca ttcaaatccc
    85981 tttccttttg gaatccttct tttttacatc agtttgattt tcgtatagta ttctttctca
    86041 tcttcacgat aaatcgcagc cccatttttt tgttgtgctt taagactgcc aagatttttt
    86101 ttatttacag acaaaagtgc ctcatcaatg ccgatttttt ttgcctcttg tagtactaat
    86161 gctaatcctt gtgtggcata tcctttttta cgataattag gggagatgcc gtaaccaata
    86221 tggccagcgc cgtttcttaa tgtttcgttc agtttatggc gcaaattaaa aattcctaca
    86281 tactcatgtt catcactttc taaaataaag acggttgcgg gaacgtaact ctcaggaaga
    86341 tcgattccta aagacgtttt ttcgcatttt tttacataat ctaaaaattc agcaaatgta
    86401 tagccataag cattgttgat aaatccactc tcatttggtc caaagctagt ttgtagctga
    86461 taagctttct ttaaatctgc ttcccatagt ttgatcagtt tcatcgtatt cacctcttta
    86521 agtaacctta ttcatttgtt ttctactata gcactattgt tgtaaatagc catgcctatt
    86581 tttatttatt gtgtcatatc gtattaagaa tcatcgaaca atacaaatat gaattacaat
    86641 aaaaaaatta tttgaagttc gttcattcgt tacttatgat ctctagaaga aagaaaacgt
    86701 gaattacttc agcgtctaga attccagaag gatagtaatc aacagaagaa ataatcaata
    86761 tccaaatgaa agaagatagc tgaatcgaga aaaagaggaa aaaagaaatg aagaaaaaaa
    86821 taaaatagtt actaatccca gagtcgttcg tttaacattg aaatcgatca tatggataaa
    86881 acagattttt tcaccaaccc tcaatagatg atggagttgc tcggatgaat tatttgtgta
    86941 gatggcataa gggagggcgt acagaaatga aaaagaacaa aaatatgttc ttgacaaaaa
    87001 acacatgtaa tcctaaacta aaagatgtga tgcggctatg gcggaattgg cagacgcgct
    87061 agcttcaggc gctagtgagg gtgacctcgt ggaagttcga gtcttcttag ccgcataatt
    87121 tgttttttta taaagagaag gtacacctgg agcgatgaga aagaaaataa catattatct
    87181 aaaagaatat aggaaaaatt ttaaacaacg gaaccagatt caaataaatc aaaaaaaatt
    87241 tgtgtgaagg tgggacagtt taaaacctaa tagattgatt caaatagtag atttatagat
    87301 tctgatacaa gaaatactgg caaacgatag ttaaggaata aagactaaac ttaaactcaa
    87361 aaagaggtca tgcgaaagaa taaatcgcat gacctcttac tatattttga atgtgtttaa
    87421 acgttcaaa
//
LOCUS       DAZKOB010000003        79424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000003 DAZKOB010000000
VERSION     DAZKOB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.003"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(381..2016)
                     /locus_tag="ACG8M6_000182"
                     /pseudo
     CDS             complement(381..2016)
                     /locus_tag="ACG8M6_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2339..2818)
                     /gene="rlmH"
                     /locus_tag="ACG8M6_000183"
     CDS             complement(2339..2818)
                     /gene="rlmH"
                     /locus_tag="ACG8M6_000183"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0455885.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3246..4535
                     /locus_tag="ACG8M6_000184"
     CDS             3246..4535
                     /locus_tag="ACG8M6_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0455886.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4574..5179)
                     /gene="ytpR"
                     /locus_tag="ACG8M6_000185"
     CDS             complement(4574..5179)
                     /gene="ytpR"
                     /locus_tag="ACG8M6_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0455887.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5384..5848)
                     /locus_tag="ACG8M6_000186"
     CDS             complement(5384..5848)
                     /locus_tag="ACG8M6_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0455888.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5870..6190)
                     /locus_tag="ACG8M6_000187"
     CDS             complement(5870..6190)
                     /locus_tag="ACG8M6_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0455889.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6334..7413)
                     /gene="pepA"
                     /locus_tag="ACG8M6_000188"
     CDS             complement(6334..7413)
                     /gene="pepA"
                     /locus_tag="ACG8M6_000188"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0455890.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7613..7963
                     /locus_tag="ACG8M6_000189"
     CDS             7613..7963
                     /locus_tag="ACG8M6_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0455891.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8186..9133
                     /locus_tag="ACG8M6_000190"
     CDS             8186..9133
                     /locus_tag="ACG8M6_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0455892.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9190..10608)
                     /gene="pepV"
                     /locus_tag="ACG8M6_000191"
     CDS             complement(9190..10608)
                     /gene="pepV"
                     /locus_tag="ACG8M6_000191"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0455893.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10757..11602
                     /locus_tag="ACG8M6_000192"
     CDS             10757..11602
                     /locus_tag="ACG8M6_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0455894.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11606..12046
                     /locus_tag="ACG8M6_000193"
     CDS             11606..12046
                     /locus_tag="ACG8M6_000193"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0455895.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12096..12290
                     /locus_tag="ACG8M6_000194"
     CDS             12096..12290
                     /locus_tag="ACG8M6_000194"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455896.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12426..13112
                     /locus_tag="ACG8M6_000195"
     CDS             12426..13112
                     /locus_tag="ACG8M6_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0455897.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13520..14209)
                     /locus_tag="ACG8M6_000196"
     CDS             complement(13520..14209)
                     /locus_tag="ACG8M6_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455898.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14184..16313)
                     /locus_tag="ACG8M6_000197"
     CDS             complement(14184..16313)
                     /locus_tag="ACG8M6_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0455899.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16361..17698)
                     /locus_tag="ACG8M6_000198"
     CDS             complement(16361..17698)
                     /locus_tag="ACG8M6_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0455900.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17950..18309)
                     /gene="rplT"
                     /locus_tag="ACG8M6_000199"
     CDS             complement(17950..18309)
                     /gene="rplT"
                     /locus_tag="ACG8M6_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0455901.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18362..18562)
                     /gene="rpmI"
                     /locus_tag="ACG8M6_000200"
     CDS             complement(18362..18562)
                     /gene="rpmI"
                     /locus_tag="ACG8M6_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0455902.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18634..19158)
                     /gene="infC"
                     /locus_tag="ACG8M6_000201"
     CDS             complement(18634..19158)
                     /gene="infC"
                     /locus_tag="ACG8M6_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0455903.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(19202..19327)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19478..20110
                     /locus_tag="ACG8M6_000202"
     CDS             19478..20110
                     /locus_tag="ACG8M6_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0455904.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20112..21017)
                     /locus_tag="ACG8M6_000203"
     CDS             complement(20112..21017)
                     /locus_tag="ACG8M6_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0455905.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21102..21761)
                     /locus_tag="ACG8M6_000204"
     CDS             complement(21102..21761)
                     /locus_tag="ACG8M6_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0455906.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            22077..23021
                     /gene="mvk"
                     /locus_tag="ACG8M6_000205"
     CDS             22077..23021
                     /gene="mvk"
                     /locus_tag="ACG8M6_000205"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0455907.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23014..23991
                     /gene="mvaD"
                     /locus_tag="ACG8M6_000206"
     CDS             23014..23991
                     /gene="mvaD"
                     /locus_tag="ACG8M6_000206"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0455908.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24004..25089
                     /locus_tag="ACG8M6_000207"
     CDS             24004..25089
                     /locus_tag="ACG8M6_000207"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0455909.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25086..26150
                     /gene="fni"
                     /locus_tag="ACG8M6_000208"
     CDS             25086..26150
                     /gene="fni"
                     /locus_tag="ACG8M6_000208"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0455910.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26399..27202)
                     /locus_tag="ACG8M6_000209"
     CDS             complement(26399..27202)
                     /locus_tag="ACG8M6_000209"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0455911.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27222..28064)
                     /gene="kduI"
                     /locus_tag="ACG8M6_000210"
     CDS             complement(27222..28064)
                     /gene="kduI"
                     /locus_tag="ACG8M6_000210"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0455912.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28080..29087)
                     /locus_tag="ACG8M6_000211"
     CDS             complement(28080..29087)
                     /locus_tag="ACG8M6_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0455913.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29094..29738)
                     /locus_tag="ACG8M6_000212"
     CDS             complement(29094..29738)
                     /locus_tag="ACG8M6_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0455914.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29838..30596)
                     /locus_tag="ACG8M6_000213"
     CDS             complement(29838..30596)
                     /locus_tag="ACG8M6_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0455915.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30723..31874)
                     /gene="fucO"
                     /locus_tag="ACG8M6_000214"
     CDS             complement(30723..31874)
                     /gene="fucO"
                     /locus_tag="ACG8M6_000214"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0455916.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31956..32267)
                     /gene="rhaM"
                     /locus_tag="ACG8M6_000215"
     CDS             complement(31956..32267)
                     /gene="rhaM"
                     /locus_tag="ACG8M6_000215"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0455917.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(32269..33114)
                     /gene="rhaD"
                     /locus_tag="ACG8M6_000216"
     CDS             complement(32269..33114)
                     /gene="rhaD"
                     /locus_tag="ACG8M6_000216"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0455918.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(33128..34384)
                     /gene="rhaA"
                     /locus_tag="ACG8M6_000217"
     CDS             complement(33128..34384)
                     /gene="rhaA"
                     /locus_tag="ACG8M6_000217"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0455919.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34381..35841)
                     /gene="rhaB"
                     /locus_tag="ACG8M6_000218"
     CDS             complement(34381..35841)
                     /gene="rhaB"
                     /locus_tag="ACG8M6_000218"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0455920.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35994..36863
                     /locus_tag="ACG8M6_000219"
     CDS             35994..36863
                     /locus_tag="ACG8M6_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0455921.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36989..37888)
                     /locus_tag="ACG8M6_000220"
     CDS             complement(36989..37888)
                     /locus_tag="ACG8M6_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0455922.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(38053..38877)
                     /locus_tag="ACG8M6_000221"
     CDS             complement(38053..38877)
                     /locus_tag="ACG8M6_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0455923.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38888..40183)
                     /locus_tag="ACG8M6_000222"
     CDS             complement(38888..40183)
                     /locus_tag="ACG8M6_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0455924.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(40199..40678)
                     /locus_tag="ACG8M6_000223"
     CDS             complement(40199..40678)
                     /locus_tag="ACG8M6_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0455925.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40684..41712)
                     /locus_tag="ACG8M6_000224"
     CDS             complement(40684..41712)
                     /locus_tag="ACG8M6_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0455926.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41729..42835)
                     /locus_tag="ACG8M6_000225"
     CDS             complement(41729..42835)
                     /locus_tag="ACG8M6_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0455927.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(43064..45661)
                     /gene="adhE"
                     /locus_tag="ACG8M6_000226"
                     /gene_synonym="adhC"
     CDS             complement(43064..45661)
                     /gene="adhE"
                     /locus_tag="ACG8M6_000226"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0455928.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45966..46346)
                     /gene="yajC"
                     /locus_tag="ACG8M6_000227"
     CDS             complement(45966..46346)
                     /gene="yajC"
                     /locus_tag="ACG8M6_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0455929.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46443..47588)
                     /gene="tgt"
                     /locus_tag="ACG8M6_000228"
     CDS             complement(46443..47588)
                     /gene="tgt"
                     /locus_tag="ACG8M6_000228"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0455930.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47806..48504
                     /locus_tag="ACG8M6_000229"
     CDS             47806..48504
                     /locus_tag="ACG8M6_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0455931.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48672..51311)
                     /locus_tag="ACG8M6_000230"
     CDS             complement(48672..51311)
                     /locus_tag="ACG8M6_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0455932.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51930..52961)
                     /gene="queA"
                     /locus_tag="ACG8M6_000231"
     CDS             complement(51930..52961)
                     /gene="queA"
                     /locus_tag="ACG8M6_000231"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0455933.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            53154..54328
                     /locus_tag="ACG8M6_000232"
                     /pseudo
     CDS             53154..54328
                     /locus_tag="ACG8M6_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            54332..54973
                     /locus_tag="ACG8M6_000233"
     CDS             54332..54973
                     /locus_tag="ACG8M6_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0455934.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            54970..55887
                     /locus_tag="ACG8M6_000234"
     CDS             54970..55887
                     /locus_tag="ACG8M6_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0455935.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55884..56555
                     /locus_tag="ACG8M6_000235"
     CDS             55884..56555
                     /locus_tag="ACG8M6_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0455936.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(57096..57584)
                     /locus_tag="ACG8M6_000236"
     CDS             complement(57096..57584)
                     /locus_tag="ACG8M6_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0455937.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57785..58051)
                     /locus_tag="ACG8M6_000237"
     CDS             complement(57785..58051)
                     /locus_tag="ACG8M6_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0455938.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(58051..58314)
                     /locus_tag="ACG8M6_000238"
     CDS             complement(58051..58314)
                     /locus_tag="ACG8M6_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0455939.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58835..58975
                     /locus_tag="ACG8M6_000239"
                     /pseudo
     CDS             58835..58975
                     /locus_tag="ACG8M6_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(59117..60940)
                     /locus_tag="ACG8M6_000240"
     CDS             complement(59117..60940)
                     /locus_tag="ACG8M6_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0455940.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61538..64243)
                     /locus_tag="ACG8M6_000241"
     CDS             complement(61538..64243)
                     /locus_tag="ACG8M6_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0455941.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64549..65496)
                     /locus_tag="ACG8M6_000242"
     CDS             complement(64549..65496)
                     /locus_tag="ACG8M6_000242"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0455942.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65686..65955
                     /gene="rpsN"
                     /locus_tag="ACG8M6_000243"
     CDS             65686..65955
                     /gene="rpsN"
                     /locus_tag="ACG8M6_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0455943.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(66131..66493)
                     /locus_tag="ACG8M6_000244"
     CDS             complement(66131..66493)
                     /locus_tag="ACG8M6_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0455944.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66504..67625)
                     /gene="alr"
                     /locus_tag="ACG8M6_000245"
     CDS             complement(66504..67625)
                     /gene="alr"
                     /locus_tag="ACG8M6_000245"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0455945.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67641..67991)
                     /gene="acpS"
                     /locus_tag="ACG8M6_000246"
     CDS             complement(67641..67991)
                     /gene="acpS"
                     /locus_tag="ACG8M6_000246"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0455946.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(68121..69632)
                     /locus_tag="ACG8M6_000247"
     CDS             complement(68121..69632)
                     /locus_tag="ACG8M6_000247"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0455947.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69954..71306)
                     /gene="murF"
                     /locus_tag="ACG8M6_000248"
     CDS             complement(69954..71306)
                     /gene="murF"
                     /locus_tag="ACG8M6_000248"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0455948.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(71459..72535)
                     /locus_tag="ACG8M6_000249"
     CDS             complement(71459..72535)
                     /locus_tag="ACG8M6_000249"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0455949.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72731..74476
                     /locus_tag="ACG8M6_000250"
     CDS             72731..74476
                     /locus_tag="ACG8M6_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0455950.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74756..75193)
                     /locus_tag="ACG8M6_000251"
     CDS             complement(74756..75193)
                     /locus_tag="ACG8M6_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0455951.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(75177..76550)
                     /locus_tag="ACG8M6_000252"
     CDS             complement(75177..76550)
                     /locus_tag="ACG8M6_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455952.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76872..77360)
                     /locus_tag="ACG8M6_000253"
     CDS             complement(76872..77360)
                     /locus_tag="ACG8M6_000253"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0455953.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(77382..77984)
                     /locus_tag="ACG8M6_000254"
     CDS             complement(77382..77984)
                     /locus_tag="ACG8M6_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0455954.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(78053..79392)
                     /locus_tag="ACG8M6_000255"
     CDS             complement(join(78053..78951,78951..79392))
                     /locus_tag="ACG8M6_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0455955.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT        22876 a        16856 c        14416 g        25276 t
ORIGIN      
        1 tagaagcatg taattttttc tcagaaacaa accactcatt ggaaaagaca tgaatcaaat
       61 catcacttac ttgtgtaaaa tagcgacgtt taatgacaaa gatatcttca ttgctatcta
      121 gtgtaccttc atttctttgt gcttaaattc aaagataatt ttctcaataa agatttttga
      181 ttccagtaag attcctttac tccttttaca acatagcgtt gtcctttgtt ttgaaacatc
      241 ttcttcccct ctttgccatt aaaaatagcc caattctcta taaagaagat taagcttcat
      301 tattataata taaagtttaa atttggttta ttacttactt tttcttattc tctagtaagc
      361 taatattgaa aaaaatgtaa tcagatcatt atttttatat gttgtaactt cctgataaga
      421 aaactaatta aaagaaagag gtaactacat aaactaatta aaaaagctaa cttcatagtt
      481 ggaggataaa tggaagaaac ttcaatgatt gtttctcctt gtgttccttc aatcgacact
      541 agtcctatta tcaatctctc ttttttgtcc atttactttt gcgactgttc cccaataatc
      601 aaatacagga agcgttattt tttgtccttt agattcatgt actgtataga ctattttcga
      661 atccatcact tgataaggca tagtatgcca ttccttgcca attcttactt catgattctg
      721 caaatgttct ttgtattgta atgcttccgt gggtgcatag tcataaagtc ctccgggaag
      781 gagtgtcgtg taacgaataa gattttcttg attcattacc aagtgatctc tctgagtgac
      841 aactttatgt tcatagtttg cttttgttgc agcagataca gccaacagta ataaaacaat
      901 tgccgttata ttaactactt ttctttgctt agctgatgtg ctcttcatga aaacgaacga
      961 taaaatcaac gagccaaaca gtacttgaaa gcctaagatt cgccaaggaa attgaatcat
     1021 ttgaatgggt gtattttgta ataaagacca tggaaaaaat ttggtggata agagccaaga
     1081 gactaccgca ctaaggtaaa tcactttttc tttgcgatta aatcctttaa aaaagaagag
     1141 tggacagatc aaagaaataa taccaatgat tcctaccgta aaggttgtca ggtcattatt
     1201 catcgcacca atcaaactat ccgagacatt taatgcttgg tgttgtaagg tcgttttatc
     1261 tggctcatta atcgattgaa ccatgatttg tcggaacatc ggataccaaa agtatgctgt
     1321 taaaagaatc gtcattccta ctgcctttga caactccaca atcaaccgga tgctttcttt
     1381 ttttcttgtt tcacttacta caaagccgcc taagactagt aagccaacgg ctattacact
     1441 cataaaagtg gaaagaacat gtgtataaat gagtaagctc atactgactg ctaatggaat
     1501 tgcacatggc tttttctcat aaatcagtgc gtaagcataa tacataacta tcggtataac
     1561 cgtaagtgca atcgcttcag caatagcaga acgatgataa atatcaatcg tacggtaccc
     1621 ataaaatgta tataaacagc taaatataaa agccgcatcg attcttttta aaaattttag
     1681 tccataatga aacatcagca tgatagtaca aacattcaat agccaaacat ataagatata
     1741 actaacgatt aaattattac ttatccaata aaaaatgact gccggaaaaa aggtcaagta
     1801 gggataaaaa taattcactc cgctcccata atgattaaac gtcgtaaaat tgattggacc
     1861 agaaaaaatt gaagataacc ctttgactct agcaaaatgg aaggcaatat cccccgatgt
     1921 agaaatagta tgaacatttc taaaataatt gattgaataa agtccagcaa taattgctag
     1981 gagaatggac aatattattt ttttctttac ttgcattact taattatata ctcctttcat
     2041 ggattgcttt atctagtata acttaaattt taaataaatc gttatttatt tttactctca
     2101 cttaaaaaag cactaaaatt ataagagagg gaggtaatgg tctgaacacc aagaattgtt
     2161 ccaacatcga attgcttaac taatgagttc aaagcttaat tatgtggctc ttatatttac
     2221 cgagcgtgga ttgcgatctc tttagcgaaa caaacatctt tcataaaaaa aacagccata
     2281 tccgctttct tggatatgac tatttactac tactttacta tctttcattc ttcttgtctc
     2341 acttatgata aggcgccccc gcattgatcc ggaaggcacg gtagatttgc tcgacaagga
     2401 tcagacgcat caattgatgt ggatacgtca tttttccaaa agagatctga gcattgcttc
     2461 ttttcatgac ggcttcgctg agtcctaaag agccgccgat aacaaaaaca aggtggcttt
     2521 ttccttggat acctaattgg tcgatttgtt tagcaaaaga ttcacttgta ggatttttcc
     2581 cttcaattgc taaggcatag acgtattctt gttctttgat tttcgctaag atccgttccc
     2641 cttctttttc ttttacttgt ctcatttgcg cttcgcttaa attctccggg gctttttcgt
     2701 caggaacttc gatcaattcg atttttgcat aggcattcag ccgtttgaca tactcattga
     2761 taccttggat caggtatttt tcttttaatt ttcctactga gataattttg atattcatgt
     2821 tttctctcct ttggtcttca tgctcacttt gtttattata atcaattttt tcgtctatat
     2881 gtttattttg gctatttttg ttaaaaagat ttccccaaca gttatgcaca acatccacag
     2941 gtttatccac agaaaacagt gttattttac cttatttcat cacattcatt aggcaaacat
     3001 acgttttata agaatttatc cactttatac acaggctgtg gatatgtttt atcccttatt
     3061 tttccaatcc tatccgcgta gttttacaca gtatctgtgg aaaaagaaaa tcaaatattt
     3121 tcatctgaaa acaatgattt ttcctaaagt ttttcataaa aagttcacag tctcaaagta
     3181 gactaaagtc aacaaataag actatactaa cagtagataa atgaatgtaa ttggaggagg
     3241 aacacatgga tagaaaaaat gtgacaccaa agatgaaaaa aagtaaaaat aatagcctct
     3301 ggcgtaagtt aggtctgggg cttgttggag ggatcgttgg aggactactg acagctggca
     3361 ttttttatgc agcgatgggc actggcaaca atagttctac tgctacaagc ggcaatcaga
     3421 attctgccgg tgaaacagtt gtggaaaatg tcaaagtcaa tgtagattct gatatcacca
     3481 aagcggtaga taaagttcaa gacgctgtcg tttctgttat caacttacaa agtcaaaacc
     3541 aatcaagcgg ttttggcgga ctattcgggc agcaagaaga aagtagcagc tcagatgata
     3601 gtaatttgga agcttatagt gaaggaagcg gcgtgatcta caaaaaagac gggaatactg
     3661 cttatgtagt cacgaataat cacgtagtag atggccagca aggtcttgaa gtcttgatga
     3721 aagacggtac gaaggtaaaa gctgaattag ttggaacaga tgcttattca gatttagcgg
     3781 tgttgaagat caattcagac aaagtcgaaa cagttgcctc ttttggtgac tcaagtgctc
     3841 tgaaggttgg tgagcctgcg attgcgattg gttccccact aggatctgaa tatgcaaatt
     3901 ctgtcacttc aggaatcatt tcttccttga accgacaagt gacaagtacg aatgaatcga
     3961 acgaaacagt caatatcaat gcgatccaaa cagatgcagc aatcaaccct ggaaattctg
     4021 gtggtccttt agttaatatc gaaggacaag ttatcggtat caactcaagt aagatcgcaa
     4081 gtacttctga atctacttct aatgtaagcg tagaaggaat gggatttgct attccaagta
     4141 acgatgtagt gaatatcatc aatcaactgg aaaaagacgg aaaagtgaca cgtccagctc
     4201 taggtatcac aatggtcgac ttatctgccg tttctactca gcaacaagaa caaatcttga
     4261 agattccttc atctgtaact aacggtgtca tcgtgacaaa tgtccaaaca gctacaccgg
     4321 ctgaaaaagc tggattgaaa caatacgacg tcattactaa gatcgatgac aaagaagttt
     4381 cttctggtgt agaacttcaa tctgctttgt ataagaaaaa agtgggagat tctgtcaaaa
     4441 tcacttacta tgaaggctcg aaagaaaaaa caactactgt tgaattgacg atcgatcaat
     4501 cggcattaaa acaaaatcaa aatagtgaaa attaataaaa aagaggttgt gacaaagact
     4561 tgtcacaacc tctttatttt ggaaatgctt ctccgactac ggcatcctca ggcaatacta
     4621 agattccttt ttttgctgga gcatttggca aatgcaattc acgagcggaa cagatcattc
     4681 catagctatc tacccctcgc aaagttcctg gccagatcat cattccatct ggcatcatag
     4741 ctcctggttt tgcaacgaca actttttgtc cagcttcgat attgggtgca ccgcaaacga
     4801 tttgcaatac tgtaccattg tctacttctg tctcagtaat cgataaatgg tcagaatcag
     4861 gatgtgggac gcatgttttt acgtacccga caacgatctt cggttcatga tcagcttcaa
     4921 gctgttcttc gaatcctgct tctttgatca atcgattcaa tttcgctaat tcctcatctg
     4981 ttaagcttac ttggccgttc ccttcgattg ttagatgatc ggagatatgg aaaaagttcc
     5041 aagcaacaac ttgaccttgt tcatctttga tccgagcaac attccatttg cgttcaacag
     5101 tgttttctgt tcctttatcg tcggctataa taaccatcaa ggtgtctccg acgtgtgctt
     5161 tgttataagc aaaaatcatg ttctttcctc cattatcctt tgctgagatt tatcttaaca
     5221 aaaaattgca ttcactgcat gaaatttttt tcatgcttta tttccgaaga agcctcttct
     5281 ggaacaccat ttattcgtaa caagggactg tgacaagact tttgtcacag tccctttact
     5341 gttttagtta attgattaaa aaggtcgtac aagttccact tgcttaatta cttggctgtt
     5401 cttcttcgtc agaaacgatc aagacatcac aaggtgaatg acgtatcacg tagctggcta
     5461 cacttcctgt cataaagcgt tcgactgcat ttagacctga ctgcccaacc atgattagat
     5521 cgatctcata tttttttggc aatttgactg ccatggattc acgaacagaa ccatatgcaa
     5581 taattccttc tatatttgtg taatcaacag atttcccata ttctttacat tcttcgatca
     5641 tttccttggc tgtttcagtt tgctgatcga ctagattttg atcaataggt gcatagccca
     5701 taaagttata aaattgctga tcgattacgt ttgctacata tactttccca ttattacggc
     5761 gagccacttc taccgctttt ttgaaagcag caagtgcttg ttcactaccg tcgataccta
     5821 ctaaaatatt cttatattct tgattcatct tgacccctcc taacagatgt cagccttttg
     5881 ttacactttc taggaacgat tcgatttcct gttttgtttt acgatcttta ttcactagac
     5941 gacctaattc ttttccgtct tggatcacga caaaacttgg gataccaaaa atgttccatt
     6001 cagcggctac atctatgtac ttgtcacggt ctacttcaat aaattgccaa tcagaaaagt
     6061 cattttcaat ttcaggcatt tgcggtttga taaaacggca gtctccgcac cagccggcag
     6121 taaagaaaaa tacactcttt ccagtttcta cgtagcctgc taattcttct aaactatttg
     6181 ggataatcat tctttgtcct ctactttctg tttatgtatt tttcaattaa attatacacc
     6241 tttctttcga actaacaaac attgatcctc tttttataaa aaaaatctgg caaaaagcaa
     6301 tcttttgcca gattttatag tttcactatc gcattatttt ccgtatacga tcgtatttac
     6361 tgttgattta tccaaacctt tcagtgtttg aatcaacatt tctcgtgcag cttcaaaatc
     6421 gcgaatactg aacatcgttt gatgcgtatg gatataacgt cccactacgc caatcactgt
     6481 acttgggatt ccttcgtttt gtgtatgagc agcacctgca tctgtaccgc cttttgaaac
     6541 aaagtattga taagggatat tgtttgtttc agcgatatcc agcaagtact cgcgcaaacg
     6601 aggaagcatg atcaaacctg gatcttggat acgcatcaat gtaccttctc ctaaatgtcc
     6661 gaatgtgcca tttttcgtca ctgtatcatc tgctgcagag cagtctactg caaagaacag
     6721 atctggcttg aatttattga cagaagcttt agaaccacgc aagcctactt cttcctggac
     6781 attcgcgcct gcaatcaatg tatgacctaa tgtttcattt tgcaacgctt ctaaagcttc
     6841 taataccatt gtacagccat agcggttgtc ccatgattta ctgataatat tctttccgtt
     6901 ggctgttttg atcgtttcag cgtaaggaac gattgtgtct cctggaagga caccgtaact
     6961 cattgcctct tctcttgatt caaacccagc atcgaacaaa atatcggtta cttctactga
     7021 tttttgtccg cttgttcctc gcaataaatg tgggggaacg gaagaagaaa tacatggata
     7081 attcttacca gtagaggttt taagtgtaaa acgctgagca gaaacaacgt aaggattcca
     7141 gccgcctaga ggtacgactt taaaaagccc gttttcttgg atttgtgtca gcatgaatcc
     7201 gacttcatcc atatgtgctg cgaccattac acggggtgca tccgcttctt catgatggcg
     7261 cagaccaaaa atgccgccca aaccatctaa ctgtaactca tctaccagcg gttccatatg
     7321 actttccata taagcgcgaa catcttgttc aaaaccgctt gttccttgta attctgttaa
     7381 ctctttgatt cgttgaaatg ttttttcttc catatgctat tcgtctaaat ttcagactaa
     7441 actcctctca gacttatatt gatctgcgga taaaatatga ttttttctgc agaccgatcg
     7501 tatttattat agcttatttt ttctggtagc ggttatttat ttcaaagaaa atatgagagt
     7561 gtggtaaaat aagcacaagg agtaacaagt gagaagaagg aggtatgtcc acatgtatga
     7621 agaaagcgaa ctaagctatt ttaaaggcgg gttagcagtt ggaatcggtc taggattatt
     7681 gagcggggta gcttcaacga tttggtatca tcgcaaaaaa gtattgtcag cagaccaagt
     7741 acttgaaaat atcaagtcat cttttctaaa agaaggtgca atcgaaggtt cttggatctc
     7801 ttttgaaaag gaacctatta ggaaattcgc cgttcggtcg agtgcttatc ggggaggtct
     7861 ttctcgaata gaagatggcg aaactgttta ttatgaattt ttagcagatg ctcatacagg
     7921 aaccgtatta gaaatttcaa gaaaaaaagg tgcaacagat taatacgtca aaaaagtgag
     7981 agaacgaata cgcacccact attcaaaaga gaggtatcat gtccttcttt tgtagtgggt
     8041 ggttttttag atagagcttt ccaaacatat attccagtat tttcttttta aataaatgat
     8101 atttagcggg aaaaactagc atttcctttg attatgaggt atttttaatt tagcttttgt
     8161 tttctatagt taaggagagt gaaaaatgga acaacaagaa aaaattgctg ttctgattcc
     8221 ttgttacaat gaggaagcaa caatttcaac agtcattgca gactttaaac gagaattacc
     8281 agaagcagat atttatgtct atgataataa ttcgaccgat cgaacatacg aattggccgt
     8341 tcaaggtgga gcaatcgtaa aaaaagaacc tcgtcaagga aaaggaaatg tgatccgcca
     8401 aatgttcttt gatatcgatg ccgactatta tttaatggta gatggcgatg atacttatcc
     8461 tgcagaagct gtacatggtt tgctcgagaa attacgttct ggtgaagcag atatggtcat
     8521 tggtgatcgc ctatcaaatg gaacctactt tgatgagaat aagagacctt tccacgattt
     8581 tggaaataac cttgttagaa atacaattac tagaatgtat aaaacaaaaa ttcttgatgt
     8641 catgaccggc tatcgaggat tcaatcgtat ctttgtcaaa agttttccta ttatgagttc
     8701 tggttttcaa atcgaaacgg aattgacgat ccatgcttta gataaaaaat tcaagttcgt
     8761 tgaaatccca atcgattatc gtgatcgtcc tgaaggcagc gaatcaaaat tgaatacatt
     8821 ctctgacgga ttcaaagtga tcatgatgat tattaaaatg tttaaagatt ataagccttt
     8881 gcttttcttt gggatttgga ccatcttctt ctttttattt ggattggtca cggggattcc
     8941 tgtgattcgc gaatatatgc tgacaagctt tattacacgc atcccttctg cgattttaag
     9001 tactggattg atgactttag cacttctttc attagtaaca gggttgattt tggatacagt
     9061 cgtcacgaac gctaagaaag aatatgaatt gaatctatat catgtttatc atgaatacca
     9121 cagtagaaag taaaaaaagg atcaagactg caaaaataat ttgcaattct tgatcctctt
     9181 tttaattaat tatttcgcca aacgatagat ggcatctgca tagattgctg ttgctctgaa
     9241 caagtcatca agactcataa attcgtttgc ttgatgcatt gtatcagtat atcctgggaa
     9301 catcgctcca taagcaacgc cacgtttcaa caaacgtccg taagttccgc caccgatgat
     9361 ttgttcgtag cctttttctc cagtgtggtc ttcatagact tgcaataatg tagccaccaa
     9421 tggatcatcc attggtacgt aatgtggttc cattacgcga gcacctcgag taactgttgc
     9481 gccttcttgc cccactgttt gttcgatgtc actttgcaat ccgtctactg ttacaccttt
     9541 tggaaaacgg aaattcatat tgatgaagtt cgcttcctct gtaccatttt cttcaaaagc
     9601 aaatagtcca gcattcattg tcaatttgcc cattttttca tcttcataag cgactcctag
     9661 tttttctcca taaaaatctt catggacata agcagctgct acatggataa attgtttagc
     9721 agatcctagg aattcatagt tatctaagaa caatgccaag aaagaacctg cattgatacc
     9781 agattgtggg cttgcaccat gtgcgccttt accaactaat tcgattttta catacgtatt
     9841 gtccgcttca atcgttccgc ttactggatt tgcttcgatg aattggtaaa atgcttcttc
     9901 catcgcgata gctgcatctg cacttggtac ttgaagtgct gcagttgctg ttcctggcac
     9961 catattttcg cgaagtccgg aaacaaaaga tttcaagaca taatctcctg cattgtcgcc
    10021 tttgaagcgt aaagctaaag aaacatttcc tttttcacca ttgatgattg ggaattcagc
    10081 atctggtgag aaaccaaaat ctggttcttc ttcatgttcg aaataataag ccatatcgcc
    10141 ccagccgctt tcttcatcgc tgccgactac gaagcgaact ttttttgata gtttcaagtc
    10201 aagttcttta atgatcttca tcgcatagta agctgccata cttggacctt tgtcatcgct
    10261 tgatccgcgt gcaaagatct ttccatcctt gattactggt tcataaggat ctgtgtccca
    10321 gccatctcct gcaggaacga catccatgtg tccaaatatc cctagcgttt cgtctccttc
    10381 gccaagatca atatgccctg catagttatc gacatttttt actttaaatc catcacgttc
    10441 gccataagct aacatatgtt tcaatgcgtc gcgaggtcca ggtccaaatg gtgcatctgg
    10501 tgttactttt gagtcatcgc gctcgctgtt gacacgtaaa agatttttca agtcttcaag
    10561 caagtcttct ttacgtgctt ctacttcttt ttgccaatca attgtcataa agttaatcct
    10621 ccacttattt aagtcttcgt gttcatgata ccacaaatta cagaaaaaaa tagttcaaaa
    10681 aatctgacaa ttccttttct tatctgtttt tctctaaaaa aaatatacgc tataatgagt
    10741 aaaaggaggt ttttagatgc tgataaacga acaactagtg aaaagagtca tcacacctcg
    10801 tccaaaaatt tcacataaag gaacatttgg acgtatcgtt ttgattggcg gcaatgaaca
    10861 atatggcgga gcaatcatta tgagtgcttt agctgccgta cacagcggag cgggtctcac
    10921 gactgtagta actgatgaaa aaaatcggag tgctcttcac agtcatctgc cagaagcaat
    10981 gattgtagtt tggacggata ctgaccagtt tctttccgta ataaagcaag cagatgtact
    11041 cttgattggt cccggattgg gtacttcttc taagagtttg gaacgtttca aacaagtact
    11101 agaaaaccaa tcaaaagaac aatggctggt gattgatggc tctgctttaa gtttacttgc
    11161 tgctaatcat cttttgctac cctatcccga acaaactgta cttacacctc atcaaatgga
    11221 gtggcaacgg ttgagttctc taccgataca agaacaacgg gataaagaaa acaaaaagaa
    11281 gcaacaggaa atcggtgctg tgctcgtttt gaaaagtcat gagacaaaaa tttatggtca
    11341 ccagcacttc tacaccaatc ctttaggttc tgctgctatg gcaacgggcg gcatgggtga
    11401 cactttagct gggatgatca caggattttt agcccagttt cctcaaaatg accaaacgat
    11461 tgcagcagct atttatcttc atagtttcat tggagaccag ctggcaaaag aacaatatat
    11521 tgttttgccg acacaaatca gcgaagcact tccgaaatgg atgaattatt tttgtaaaaa
    11581 atcaatggaa aacgaggcat gagtcatgga gaaatttaca ggttatgtgc gagaaccttt
    11641 ttattatgaa acagatcaaa tggggattat tcatcacagc aattatatcc gctggtttga
    11701 agaggctcgg gtagccttat tggtacatct agggtttccc tataaaaaaa tcgaagaatc
    11761 tgggatcatc atccccgttc tggaagtaaa ttgcagctat aaagaaatga tccattacgg
    11821 agaaaaagtg agcatccatc caactatcca aaagtatacg gggacacggt tggattttag
    11881 ctacgaaatt gtgggaactg aagacaaaaa attaaaaact actggcagta gtaaacactg
    11941 ttttttactt tctgattcgc aacgattagt gaagctgagc aaagtcaatc cggagttaga
    12001 tcagttattc caagcgtacg cttctttgga tgatgtaaat tcttaataaa gaccgtctaa
    12061 gcatttttct tgtattgtgt cgagtagttc gtataatgaa aagaaaaaag caggtgatcc
    12121 ccatgtatgt cgttaaagta atgcacggtt acatcgataa aactggctgt cgcactcggg
    12181 aaaaaaactt agacaatctg ctcattttca aagacaaaaa agaatccgaa gcttttgcta
    12241 aaagaatcgg cggtcgtgtc aaaccgatcc aagaggtacg accagattga ttcaatattt
    12301 ttcttttcga ggctctaata ggcatgccta tcagggcttt ttttgcagac tgtgttttta
    12361 tgcccacacg atttgtaagc gatttatgtt ataatagatt atatctgcat agaaaggaag
    12421 gttaaatggt tatcaataat ttgcaacagc aagcttatga atctatacgt aaacaaatca
    12481 tttattccga gttagcacct ggaagtaaaa tctccgataa agtgttagaa gaacaactag
    12541 ctatcgggcg tacaccgatt caagaagcac tgatccaact gcgcaatcaa gagcttgtaa
    12601 aaaccattcc acagtccggc acttatgttt caaaaatcga tatccgatct gcttcattag
    12661 cccgttatac gcgggaaaag ttggaaaaac cgatcctgca agaatgcagt gcgaaaatga
    12721 ccaagcaaga tcaagctaaa ctagaaaaaa tactagaaca aaccgatcag gcagtaatcg
    12781 ctcaaaataa aaaagctttt tttgatttgg atactgattt tcaccgaact tgttatgaaa
    12841 ttgctggaaa acgagaaatt tgggattggc tagaaagtta tagtacgcac ttgaaccgtt
    12901 tcagatggtt acgtttgacg atttcagaat tagactgggg tcgagtatta gatgaacatc
    12961 agacaatgct tcaatcaatg attgatcata actttgatga agtcggattt ctttgcacga
    13021 tgcatttaca tatgattatc gaggagcaag aatacgtgat tcataattac cccgattatt
    13081 ttgaagatat tcaggacaga cccatcaaat aagaggtttt ggaatcagcg ttttgacctg
    13141 agtattagag aaagaaacgg caaaatagtt cctcatattt tgccgttttt tttgagctaa
    13201 taccgagggt cagctgattg cacaccgttt attgagaggt tgtgaaaaag ctgccctgca
    13261 tcaagtaata agaacagcca agtaaaaata gcttcttatg tttttaactt ggctggactt
    13321 attaccgcag atgcaagctt ttgaacaccg ttttccaaga ggtcgtgagc ctgccgttca
    13381 tctccgagtc acaaggaaca atttcaaaaa acagcttctc atgtttgttg aatattttga
    13441 cttgtgaccg aggagttgac aggagaacat cgtttattcg gaaaaaaagg actgtgacac
    13501 gacttttgtc acagtctctt tatttgtaat ctaaatcata ctccgtacat cttttttcta
    13561 ttttttttat ttcttctaag atcccttggc tacgaatttc attatatggc caaagtaaga
    13621 ctgcctctaa tacataaggc gaaaaatcgc tttgattcat ctcttttgaa aaactcatcg
    13681 ctgctatctc gctcaatgca cgaatgtttg attgatgtgt ataaaaaggc aaccgccaca
    13741 gcacaatttt ttcgctttgc gtataaattc ccttaacaga agcttcgatg tggtgaaata
    13801 gttcatgtcc aataatcaaa gcttgcgctg tttctttaga gatcgaagct agtcctgaac
    13861 attttaattt ttctaacggc tccgtcatca atcgaatccc ttcatcttct gcataagttg
    13921 caaaagtgat tcgttcgttt atttgctctg attcacagga aacgattgga acagccattt
    13981 gttcacaaat ttctcctaca tcggcaaaag ggtacttttt tttgatgcta catgccgttt
    14041 cgataccaca atcgattgat tgttgtataa gaattttctg cttggacaaa ggaatctttt
    14101 tccaaaataa atctcgccga aactggtaga atccccaaat ttcttcgggg atctgttgta
    14161 attcgtttag taactcttct aacttatgtt tgtttgatat ttccggcaac gacgatcact
    14221 tcttcctccg gttccatagt agatacttcc agatccatca aaagctgtag ttgttccaga
    14281 tccgtcgctg taaaatcaga gactcttgct cctaagcaga gataaaattc tggtcgggca
    14341 atcaattcag tttgataaac tgccatatct tcccaaagtt gttcgacagc tgtcatataa
    14401 ttggctgcag ttgttttcat acatgaagca tgtccacgtt ggaccttgat aaatcctttt
    14461 tgatcgatga tccgaagact tccagcattt tttgaacgat tctgttcgcc gcatacataa
    14521 aagaaaggtg tattttccaa taagcaaact tcatttttat ttaatcgcat atcctctgca
    14581 gcaagctcta gtgcttcttc tgttgtgatc tccttggtta gatcggttgc tttcacttcc
    14641 gtagaaccag tagcgatagc cgttactttt gaagtctgtg gatcgatttc tacgtggact
    14701 tcgatcgatt ctggtgtagc gcctgattca atcgctttat tcatcgcttc atttttcaac
    14761 gatcgtatat cttctttact tggcgaaggg atgatacgtt ctacaacatc tcggaccatt
    14821 gcaagtgcta caccaataga agaaatcact tcggcgtttt caggaatcga atatttcaca
    14881 cccattttat tactaaagta agtaatcaat gaggcagcgc cgccccctac acctacaaga
    14941 gaaatctgat ctttctccaa gtgatacttg tctgccaatt ctaaaatcac cggttcaatc
    15001 ttagcatagg atttttccat gatctgttct gcgatgtctt caaccgtcgt atgacaataa
    15061 tcagctaatg cttggatggc ttttcgtgca gatgggacat ttccataaga aaaatgttct
    15121 tcttgcacta atccaaggac attggctgca catgtatttg taatagtcac ttcttctccg
    15181 tcttccatca ctaccttgac gtaatcagca gggtctcctt ctttaggaga gaaaaattca
    15241 accttaggac ctttgatttt ttctgtctcc gtaaatactg cataatctag accggcaata
    15301 tgtgcagaac gtggaccgac atcgatgatt cctgactgat tagcccgtac catcgaaccg
    15361 cctgccacac ctagaacccg aacatctaat gaagagatat atgttgggtg gccacctaca
    15421 attgaataat cgattgctgg acgtccattt ttaatgaccc cgatattggt tgttgttccg
    15481 cctacttcaa aataaacacc attagacgct ctcaagtaca tcaaagagcc catgactgat
    15541 gctgctggac cggaaagcat ggttaaaaca ggtcgttttt tcatttcatt gatttccatt
    15601 actccgccgt ctccgcgcat aatcatcaaa gaaacattga cccctgcttc tcgtacggaa
    15661 tcttctgtag aggttgccgt atctagcatt tttggtaaaa tagatgcatt gattgctgct
    15721 gtccgagttc ttcttgtcaa tccatataat tttgtgatgt cagaagccat cgttgtaggg
    15781 atcgatttta ccgatgctgc ttcataaaca actcgttctg gttccccatt atctactcca
    15841 aatgccatag aagaaaccaa gacctccgct ctttctcttt ctaaagaaga aatcgtttct
    15901 gaaacacggt ctacatttaa gtgtttgaca ggtaagaatg cattgacaat ctctattttt
    15961 ttcttattcc ctaaatcgat atcgttcaat ctagtttgtc gttttgctaa aaaaccttct
    16021 aatccacctt ttgccatccc gattacgccg acttttgcta cgtctccttc aatcagtgca
    16081 ttagtcgctt gtgtggtaga atgtgcaaca aacacaacat cttctggact gatattattt
    16141 tccagcaaac aattttgaaa agactgaacg acacctgcgg ctactccacg gacatcatcg
    16201 tgggttgttt tcactgaaga ttttccgatg atttcgtgcg tcgcattatc gattgcgact
    16261 gctttcgtat gtgtaccgcc aacatcaatt cccattcgca caaaacgttt cattttcttt
    16321 tcctccattc atctaatcgt ttttatcgac gttttgttta ctaggcgttc ccgtacaata
    16381 agaacgtgat caggcataag atcgctccga tgatccaacc agttgggatg gatttcttca
    16441 tatattctcg agaagttact tttgtataac ctaatcccca agcaacccaa gattgtgtaa
    16501 tatctaaatg ctgaggagca atcgttgtga ttgcaaataa cgggaacaaa aaggctggcg
    16561 acatcgttgg attaacagct aatacgacag ctaaaatagc tgagcctgaa ccgactagat
    16621 tcattggacc gcggaaaaat cctagtggtg tcaatatagc aaatacgatt gccaagacta
    16681 atggtgattg cggtatccaa tctccaaaaa tagctgaaaa ataaggagaa gcatacgcag
    16741 ctgaattatt gaacatcgct aaggtcatca agaaaccaac cattggcgca acatctactg
    16801 cgccatctgt aaataatttc gcaaactgac gacagatttc tttataacct ccgcgcaatt
    16861 ttccagtaaa cagcaatgcc cagatacctg ctagaataaa acctaagatg attggaagat
    16921 ctaaaagaac gacccctaaa actggtaata atacggctaa ccatgagatc attggtgcgt
    16981 cgccaccttc agctggtacg tttgctgcca ttgcatagcg tttctttttg ttcattgata
    17041 tattagcgac cgttagtacc acaactaagc ttacaatcat cccaatcata ccaatttgga
    17101 aatagtcatt ataagtatag ctttcagccg ctggattgaa accagcataa atcgtttgat
    17161 attgtttgaa attgacgata ttagcaaaga ttccagccat aatcgagccc ataaatgaga
    17221 acagtgcaac cggtactgaa atacctaatg acatcaagat tggcaacaca ataactgcaa
    17281 tagaaatcac tggtccgatt cctgtcatcg acatgaatat aacagcggtt acaatacata
    17341 gtaagctcat cgtgattctt ggcttgtccc ctccaagttc aactaccttt cgaatcaggg
    17401 tagctgcgat tccgctatct actagaacac gcccgaaaaa ggcaccaaag aagacattta
    17461 ccaagatcga tttggcgtac tcagctggcc ccgtcgtata gatatgcgtc aacacatcaa
    17521 ttactccttg cccttccatc gccgggtttg gtgaaaacgt gtttccgact agcgctaatc
    17581 ccatccatac agtagccatg atggcaaagc cgaccatcaa gttgtagcct tttacacagt
    17641 accagaccat gaaaaagaat gagataacta acaaaatacc aataataata tcactcatgt
    17701 tattcctccg tccattttgt ttttctgata agcaaactaa tatatcagtc aaactcattc
    17761 taacttatgt agttagcgct ttcattaaag ggtgaccata aatgttcagt tttcaattaa
    17821 aaaaccttaa ttatgctatt ttagacttat acgttattgt cctgtttcat ttgatggagg
    17881 attgtaaata aaaaaagagg aaagaacata acttttcgtt atcttctttc ctctttataa
    17941 ttgtttagct tatttagcca aagcttcttt tgcttggtca gccaatgcag taaatgctgc
    18001 tgcatcgtta acagccaagt cagctaacat tttacggttg atgtcgattt cagcaacttt
    18061 caaaccgtgc attaattttg agtagctcaa gccattcata cgagctgctg cgttgatacg
    18121 agcaatccac aatttacgga agtcgcgttt cttttgacga cgatcgcgat atgcgtagtt
    18181 gtatgaattc attacttgtt cttttgctgt tttaaataaa gtgtgttttg aaccgtagta
    18241 accttttgct aattttagca ctcttttacg acgtttacga gttactgttc cacctttaac
    18301 acgtgccatg tataattcct cctaataaaa ataaatcttc agattctgtt atttgtactt
    18361 cttatttcat ttttgctagt tgttggcgaa tacgtttgaa gtcgccacta gaaaccatgc
    18421 ttgctttacg caattgacgg cgttgttttt ttgttttgcc gtggaaacgg tgacttgtaa
    18481 acgcgcggaa gcgtttcaat ccgcctttac cagtacgttt gaaacgtttt gctgatccgc
    18541 ggtgtgtttt ttgttttggc atgactatat ttcctcctca aaatctttcg ttatccgact
    18601 atcggctgtc caacagccag ttagtcattc aaattactta ctgtcgtttt tcggtgccag
    18661 cgtcaagaac atgctgcgtc cgtccatttt cgctttttgt tccactgttg caatatccgc
    18721 agtttcttca gctaagcgat tcaagacttt ttgaccaatt tctttatggg taatggcacg
    18781 gcctttgaaa cggatagaag ctttcacttt atcgcccttt tccaagaact tacgtgcatt
    18841 acgaagtttt gtattgaagt cattcaagtc gatcgtagga cttaagcgaa cttcttttac
    18901 attgatcact ttttgttttt tacgcgcttc gcggtctttc ttttgttgtt cgaaacggta
    18961 tttaccgtag tccatgattc gcgcaactgg tggtttcgca ccaggggcaa ctaacacaag
    19021 atccaagttt gcttgttctg caatattcaa tgcttctgct tttgtcttaa ctcctaattg
    19081 ctcaccatct gaaccgatca atcgtaactc tcgtgcacga atgccgtcgt taaccatcat
    19141 atcctttgct atggtcattc acctccaaat attatgagag aaagaaaagc ttaccgaata
    19201 aacggcaata aaaaaacgag ttctcttaca gaacccgcgc gtacttcacc taatagaacc
    19261 agtgaaaact atctagccca gtgacattat caactaaggc gagaagcggg tgcttctgct
    19321 tttatttctc aaccatcata gtatagcaaa aactaatctt tttgtcaatg tttttcatac
    19381 agtttgtctt ccttttctga aaatgattag attagcagac tttcttgcag tgattcccgc
    19441 ttctttgcta tactaaagaa aaacgaggtg aacccacatg aaaaaaatcg aacctattcc
    19501 accttctctt cctcttctta caaagggaga gttttattta gaagatgagg agatcttctc
    19561 tggtcttctg atcaacgggg gtgaccaaag ctatctcgat gccaaaaata tggtattaag
    19621 agagagtcat atccaaaaac ttacgatgca aaaaacagtt ttacgtcatt ttgaatgcag
    19681 taatgtcatt tttgaaaaat gtgatttttc caatctggag tggattggtg gcagtttcca
    19741 ccaagtcgtg tttcatcaat gtaaattaac aggtacgaat tttgccgaaa gttatcttcg
    19801 agactgtact ttcactgatt gtattgcttc gatggcttcc tttagtagta caaacttgaa
    19861 agctgtctcc ttcgatcatt gtcaattaga agacagcgaa ttttatgagg tcacttggaa
    19921 aaaccttttt ttgtctgaca atcagttgac cggttcgaac tggtttcgaa cttctctgaa
    19981 gggattagac tttacgacta atttcttcac agcaatcgct ttatctcaag attctttggc
    20041 gggactaatc gtcaaccaag agcaagcact tgtgatcgcc caagcccttg gtttgcagat
    20101 caaagactag atcaacgaat ccgccgccac agtgcaataa gcatccaaat agataagacc
    20161 aaactgatga tcaaggcgat tccccatccg aaaacggaat gggtaaaggg taactggaga
    20221 ttcattccga agaatcctgt cacaatagtt ggaacggtca agatcagcga ccagaccgtc
    20281 aaaaatttca tcgtgtcatt caagttgtta ttcagcaaat tattataagt gccagagagc
    20341 tggtctaaaa tctgcgaagc taagttcgtc atttcaactg cttgtcgtgc ttcgatcagc
    20401 gcatcgtcca actcttcttt ttccttttct gacaacttgc gataaatagc caatgccttg
    20461 atttgttcta aaagtactac attttgtttt gttcctgtaa ctaagtaaac caaaccgatc
    20521 tctaaatctg aaagttgcag cagattcttg ttcgttgttt tttctcttaa tttcagatta
    20581 agccgctgac gttcactatt cacttcctcg attaaaggaa aaaaggaatc agaaatcaaa
    20641 aacaatgtac gaaacaataa actgtataca ctcatgtctg gcattcgttc caaaagcgat
    20701 cgaattagtc gaatcacgta ctcggtattc ggcgtggtaa atgtaaacaa accgtcttct
    20761 tttaaaataa aagcgatagg actagtttca taatgattat cgactttttg ctggtgcgga
    20821 acatttgata caagcagtaa cgcttcatca aaagaatcgt actctacgcg cgcccgttca
    20881 tttttatcta gagaataagt cagcatctcg tcactaatgc catattcttc ttttaagttt
    20941 ttaacagcta gcacatcgtt ttgctgtaca ttgatccaaa aattacggtc ttgattaaaa
    21001 tagtgttttt cccgcatact ctatcctcct gacatttgtt tccagtataa agaaaaaagt
    21061 cgctcaaata aaggtttacc ctttatttga gcaactgtaa atcaaatggc tactttggtt
    21121 tggcgaatct ctgctaattt ttcttcgatc attttcaaga cttcttcttt tgcttgagga
    21181 tcttccacaa aattatagcg gtctccatcg atttggattt ttggactttc tttgtattct
    21241 tcataccatt gatcgtaacg tctgttcaat tctttgtaat aatcgtatag acttggatca
    21301 aaggacaatt gttcatacga acgaccacgt ttttcgatac gctccaacat cgtatcaaaa
    21361 gatacacgaa tatgaactaa taaatccgga tgttttttat gggcagcata gggaagttct
    21421 tccatcatgt tttccagcag ttcatcatac acacgaactt ctgtttctgt tgctcttcct
    21481 agatctgcat ttagatggaa aagcagtgaa tcttcataaa tcgaacggtc caatacatta
    21541 ttgtcatctt tcattgcttg tttgatgctt gcaaaacgtt tattcaaaaa ataaatttgc
    21601 agcaaaaaag catactgttc agggtttgca taaaataagg gcaagacttc attatcatca
    21661 atcgattcat aaaatgcctg actatcaaag tgttcagcca tcatttctgt taaacttgat
    21721 tttccggcac caattgtacc tgctaaaacg atcacgctca tttacctcca gctcacttca
    21781 tcactaaata ttgtgtttaa ctccaacctt tttaaatata cccccaatat ctagtagtgt
    21841 caattgtatt tttttctagt caaagaagaa aaaaagtcaa aaaattctta agaaaaaagt
    21901 gttttttctt tagaaaacgt attcttagtt ttacttctga gatggtttta gtaaaataaa
    21961 ggaggaatgt tttttgagac ccttagtgga caaaaaaatt cgtgaagcag cgcaactcgt
    22021 tttgggcgct ttattttttg cttaaaaatc acttttacgt gagaaggagt tagattatgg
    22081 caaactatgg ccaaggagaa tcaagcggaa agatcatatt gatgggcgag cacgcggttg
    22141 tttatggaga accagcgatt gcctttcctt tctatgcaac aaaagtcacc gcattccttg
    22201 aagagctgga tgcaatggac gatcaactgg tttcttccta ctattcagga aatttagccg
    22261 aagctcctca tgcattaaaa aatatcaaaa aattattcat tcacttaaaa aaacagcatg
    22321 acatccaaaa aaacttgcaa ctgaccattg aaagcacgat tcctgctgaa cgtggaatgg
    22381 gatcaagcgc tgcagtcgcc acagcagtca ctcgtgcttt ttatgattac ttagcatttc
    22441 ctttgtctcg tgaaatacta ttagaaaatg tccagctttc ggaaaaaatc gcccatggta
    22501 atcctagtgg aatcgatgca gccgctacta gcagcttgca gccgatttat tttacaaaag
    22561 ggcatccttt cgactacttt tctttgaaca tcgatgcttt tttgattgtc gctgatacag
    22621 gaatcaaagg acaaacaaga gaagccgtca aagatgtcgc tcacctcttt gaaacccagc
    22681 ctcatgaaac tggacaaatg atccaaaaat taggatactt gacgaagcaa gcaaaacaag
    22741 caatcattga aaattcacca gaaacgttgg cacagacaat ggatgaatca caatcacttc
    22801 tggaaaagct gacaatcagc aatgattttc ttaatctatt gatccaaaca gcaaaagata
    22861 ccggggcatt gggtgccaaa ttaactggcg gtggacgcgg tggctgtatg attgcattag
    22921 cacaaacaaa aacaaaagcc caagaaatca gtcaagcact tgaagatgcg ggtgctgctg
    22981 aaacttggat acaaggatta ggagtacata cctatgttta aaggcaaagc acgcgcatat
    23041 accaacattg ctctaatcaa atattggggt aagaaaaatg aagaactcat ccttccaatg
    23101 aacaacagcc tttcgttaac attggatgca ttttatacag agacagaagt catcttttct
    23161 gatagctata tggtagatga attttatcta gatggcacct tgcaagacga aaaagcaaca
    23221 aaaaaagtca gtcagtttct tgaccttttt cgtaaagaag ccggcctctc actaaaagct
    23281 tcagtaatca gtcagaattt cgttcctacc gcagctggat tagcctcctc tgccagcggg
    23341 ctagctgctt tagcaggcgc ttgcaatact gctcttaagc ttggattaga tgatctctct
    23401 ctttcaagat ttgctcgacg cgggtctggt tcagcttgcc gaagtatttt cggtggtttc
    23461 gtcgaatggg aaaaaggcca tgacgactta agttcttacg ctaagccagt cccttccgat
    23521 tctttcgaag acgatttagc aatggttttc gttttgatca acgaccagaa aaaagaagtg
    23581 tccagcagaa atgggatgcg tcggacagtc gaaacatcca gtttttatca aggctggtta
    23641 gattccgttg aaggggatct atatcaattg aaacaagcaa tcaaaacaaa agatttccaa
    23701 cttctcggag aaacgatgga aagaaacgga ctaaaaatgc atggaacgac tttagctgcc
    23761 cagcctccat tcacttactg gtctcctgac agcttaaaag cgatggatgc ggtgcgacaa
    23821 ttgagaaagc aaggcattcc atgctacttt acgatggatg ctggccctaa tgtcaaagtt
    23881 ttagtggaaa acagccattt atctgaggta caagaaacat ttaccaaatt atttagcaaa
    23941 gaacaagtaa ttactgcaca tgctggtcca ggaattgcga ttattgaata aagggagatt
    24001 tttatgattg aagtatctgc accaggcaaa ctctatattg ccggagaata tgcagttgtt
    24061 gaaacaggcc atccagcagt tatcgctgca gtcgatcaat tcgtgacagt cactgtagaa
    24121 tctgcacgaa aagtcggaag tatccaatct gcacaatata gtgggatgcc tgtacgttgg
    24181 acaagacgca acggagaatt agttttagat attcgggaaa atccttttca ttatatcctt
    24241 gctgctattc gcttgactga aaagtatgca caagaaaaaa acattctttt atccttttat
    24301 gatctgaaag taacaagtga gttagacagt tcaaatggcc ggaaatatgg attgggttct
    24361 agcggtgccg tgaccgttgc aacggtcaaa gccttgaatg ttttttacgc gttgaattta
    24421 tctcagttgg agattttcaa gattgcagca ctagccaatt tagcagttca agataatggt
    24481 tcctgcggcg acatcgctgc tagctgttat ggtggctgga tcgctttctc aaccttcgac
    24541 catccttggc tccaagaaca agaaactcag cattctatca gtgagttact tgccctggat
    24601 tggccaggtc tatccattga gccattgatt gctcctgaag atttacgttt attgattggt
    24661 tggacgggta gccctgcctc tacttctgat ttggtcgatc aagttcaccg ttcgagagaa
    24721 gataaaatgg tggcttatca gcttttctta aaaaatagta cagaatgtgt caacgaaatg
    24781 atcaaagggt ttaaagaaaa taatgtaacg ttgatccaac agatgattcg aaaaaaccga
    24841 caattactgc atgatttatc tgcaatcact ggggtcgtca tcgaaacgcc tgctttgaac
    24901 aaattgtgta atttagctga acagtatgaa ggagccgcaa aatcttctgg tgcaggtggg
    24961 ggcgattgcg gaatcgtaat tgttgaccag aaatctggca ttcttccttt aatgagtgca
    25021 tgggaaaaag cagaaatcac tccactgccg ttacatgtct atagcgatca aagaaaggaa
    25081 aaccgatgaa tcgaaaagac gaacatgttt cactagctaa agcctttcat gataaacaaa
    25141 aaaacgaatt tgacttcgta cggatcatcc ataacccttt gccgcaaata gcagtttctg
    25201 atgtcgatct aagtacacag gcagttgggt ttacactaag cagcccattt tatatcaatg
    25261 cgatgactgg cggaagcgaa aaaacaaaaa aaatcaacca agatcttgca atcgtcgcaa
    25321 gagaagcaga tttgatgatt gccactggtt cagtcagcgc tgccttgaaa gtcccttctt
    25381 tagccgatac ttatacgatt atgcgacaag aatatcctca cgggaaaatc attgccaaca
    25441 tcggtgcagg gacttctgtc gaaagagcca aagaagctat ccgattgttt cacgcagatg
    25501 ccttacagat tcatttgaat gcacctcaag agttggttat gcctgaggga gaccgtgatt
    25561 ttaccaattg gaaagtactt attcaagaaa ctcaaacagc cattgacgtc cccctcatcg
    25621 taaaagaagt cggttttggc atgacaagag aaacgctcaa tgatctagct gctttaggag
    25681 ttcatacagt tgatatcagc ggtcgaagcg gaacaagctt cacccagatc gaaaacgccc
    25741 gtcgctccaa gcgggaactg agttatttag ctgactgggg gcaatcgaca gtctcttcat
    25801 tgcttgaagc aaatgaagca gacacttcta tggaaatcct agcctccgga ggtatccgca
    25861 atgcctatga catatttaaa gccctttgtc tcggcgcaaa tgccgtgggt acttcaggaa
    25921 ccgtactgac gcatctgatg aatcacggcg tagaagaaac tattatactg atgaagcaat
    25981 ggcaagaaga gcttcgtctg ctttatacta tggtcggcgc aacgaatacc gcggccctac
    26041 accaacaatc tctgatcttt tccggaccag tgaaggattg gtgcgaagca agagggatcg
    26101 acttagtgaa gtatggaaga cgcacagaaa agagcgtggg acaaaagtaa agaacacttt
    26161 tgtctcacgc tctaaacacg tacaaacggc ggaagtaaaa gcaactcctt cggaaataag
    26221 ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt ccttcttatt tcttggagtt
    26281 aaatgcttct gtcccagcct cttttttcga ataaatgatg tttaatcagc tgtccttcgg
    26341 tattcgttca atccgctgat ttcacaacct ctccactaaa cagtgtacta aagctctttt
    26401 aaggttgttt tccgatatag gcaagtatgc ctccgtccac atacaagaca tggccgttaa
    26461 cgaaatcaga tgcgctagaa gccaagaaga cagatggtcc agccaaatcg attggctcgc
    26521 cccagcgtgc tgctggcgta cgtccaacga taaattgatc gaatggatga cgttcaccgt
    26581 tttcttgtgt ttcgcgtaat ggagcagttt gcggtgtagc aatataacct ggtccaattc
    26641 cattacattg gatattgtat tgaccgtatt cagaagcgat gtttttcgtc agcattttta
    26701 atccgccttt tgccgcagca taagcactga ccgtttcgcg tcctaattca ctcatcatgg
    26761 agcagatatt gatgatttta ccgccgcctt tttcgatcat atccggaata actgcttttg
    26821 aaacgatgaa tggggcattt aaatccacgt cgattacttg gcggaagtct gccgcatcca
    26881 tttcgatcat cggtgtccgt ttgatgatcc ctgcattgtt gactagaata tcaactgaac
    26941 ctacatcttc tttgatctgt tgaaccatcg cttgaaccgc ttcttcatcg gtcacatcgc
    27001 agacataacc ttttgcatcg atgcctgctt ctttataatg cgctatacct tcatcaactg
    27061 attcttgatt caagttatta aatacgattt ttgctcctgc tgtagctaaa gagcgtgcaa
    27121 tctcaaatcc aataccatag acagcaccgg tgactaatgc tacttttcca tctaaacgaa
    27181 acatatccat actaaattcc atcattttct ctcccttatg cttattttaa ttgatccatt
    27241 tgcaccatat ccatgtctgt ataggtgata ttttctccgc acattgccca gatgaatgag
    27301 taattgcttg tacctacacc tgaatggatc gaccagcttg gcgaaataat ggcttgttca
    27361 ttgtctacaa ctaaatgttt tgtttcatct ggtttgccca tcatgtggaa cacacgtgta
    27421 tcttcattgg cataatcaaa gtagacataa gcttccatac gacgttcatg tgtatgacaa
    27481 ggcatcgtat tccaagcgct tcctggttct aaaatcgtat aacccatttg taattgacag
    27541 ctttcgcata catttggatg gatgtattgg tagattttac gttcattcag tgtcaggcct
    27601 tcaccagtct ccattggttt tacttggtcg atactgattt ttacatttgg atatttatga
    27661 tgagctggaa cagaactaat atagaattta gctgggtttt cagcatcttg agaagaaaat
    27721 acaacatgtc gtgtttcttt tccgatatag tatccgtctt gttttttcat ttcttctttt
    27781 tgcccatcga tctcgatgaa tcctggtcca ccgatattga tcacacctag ttcacgacgc
    27841 tctaagaagt aatcaacacc tagttctttg tctaatgtga tttctaattc tttttcagta
    27901 ggtgtcactc caccaaagat cattcgatcg ttgtgcgtat aagtcaaact gattttccct
    27961 ggttcaaata ccttttctac taaaaattct tttctcaatt gttccgttga ataatgacgg
    28021 atatcttctg gactgtgtgt ataacgtgtt tccatttctt gcattttctt ctactccttc
    28081 tatcgaatga tttttccaga gccgcatgct aaaaattgtg cgacttcttc ttgactaaat
    28141 tggttgcagt ccccatggat cgtatgcttc aaggcagaag ctgccgtagc aaaatcaatg
    28201 atttcttgcg gagacatatt ggataaaatc ccatggagca cgccacctgc aaaagcatct
    28261 ccgccaccta ctcgatccac gattggggtg attctatgga cttgggattc cacatattca
    28321 tggttcatcc acaatgtccc cttcaattca ttttcacttg ctgaatgaac ggtacgcttg
    28381 gtcgaataaa acacctgaat attcgggaac ttttcctgca ttttttgata ataataaatt
    28441 agaggttgtt cttctgcttc tggcgctttc tcgataccta acaaataaag agcatccatt
    28501 tttccagctg agcagatatc tactaaaggt aaaagctgat ggattacttc gctggcttct
    28561 tgctgtgtcc ataattttcc acgatagttg atatcgaagc tgatcaggca gccagctttt
    28621 tttgcagctt ctatcacctg tttcgtcatt ttttgccatt ttttagacaa agctggggtg
    28681 attcctgaaa catgaaatat atctattcct tggaataact cctctagcga ccattccaaa
    28741 tgatccattt cagcaaaact tgatccggca cgatcataga tcactgaggc ggcgcgttcg
    28801 ccactaccag tttccatata ataagtacct agtcttgatc caccagataa tacctgtttc
    28861 gtatctactc cataacattg caagtgcttt ttgactgctt ttcccaatgc gttatcaggt
    28921 acttttgtag caaaaatcgc ttcatgtccg taattagcta aagaaattgc tacgtttgct
    28981 tcccctccgc cataatgggc agcgaatcgt tcacttgaat cgatacgggt tccctgctct
    29041 gtggacaatc gcaacatgat ttctccgagt gtcactactt ttcccataag cagctacctc
    29101 ttgatttctt tcatttttgc tgcatactgt tgcgcgactt ccgttacttt gtcaaaatct
    29161 cctgtagctg cgggtgctaa aaggttccca cctactccga ctgttacgac accagcatcg
    29221 aaccactctt tcatattgtc cagactcaca ccgccagttg gcatgatatt gacttgaggc
    29281 atcggcgcct tgaaggcaga aatgatactt ggtccataag cgcttccagg aaatagtttg
    29341 acgatatcta ccccgctttt cagcgctgtt ttgatctcgg taatagtcat acatcctggt
    29401 aagtaaggaa tttgatacag attgcacatc tctgccgttt cttgatcaaa ggaagggcta
    29461 acaatatact ctgcgccagc taagatagcc aagcgagctg tgacagcgtc taatactgtg
    29521 cctgcgccaa tcactatttc tggttggtct ttgtagaatg acaaaagttc tttgattact
    29581 tgatctgctt gagggactgt aaaagtcaac tcaataccag ttagaccacc tttgatgatt
    29641 gcgtggcaag cattcaaagc ttcttctttg ctttttccgc gtaccaccgc aatcactccg
    29701 gcgtttttca atcgagataa aatatcaatt tttttcatct tctccaccta tttcctattt
    29761 agaaatttta ttttataact tggaaatcta tacccatatt atacggcaga aaagaaaaat
    29821 tgcaaacgct tttttcatta tttttgaatt ctttgctcga tttcgctttt tgtttgcttg
    29881 agggcactga ttatttgttc ttttgtctca tctgttaaac gatatttcgg catacttaca
    29941 ctaaatgcac caatgatttg ttcgtttttc acgattgagg ctcctataca aaaaatatca
    30001 cgttcgattt cttcatcgtc aaatgcgatg tgggtttctt ttaccgtttt gatttccttt
    30061 ttcaaccgta atgcattggt gatcgtatat tctgtgtaag ggatcaacgg atgcttttca
    30121 agataatcag tgtactgctc ttcactgaat tctgctaata cggctttgcc catcgcagaa
    30181 ttgtataatg gtcgggtgat tccgattttt gaagacatgc ggatcgtttg atgttttgga
    30241 tctagtttgt tcacgtacaa gatctcattg ttgtccaata cccctaagtg gatagtctca
    30301 tcgatctttt gctgcagctg ttctaaaaac ggtaaagtca actctaccaa atcgatttgt
    30361 tcaatgtttt tattcgcgta gcgaataagc tttgcaccta aacgatagtt tttctctgaa
    30421 tttttattta cgtagccgat catcaacaaa gtatctagta ttttcaagac tgtagaagag
    30481 gtcattccca caccctttgc gatcgtctgc aaagagacat cttggttttc cgctaaatag
    30541 tctagtattt tagaggcttt gatcaataca gttccataag gctttttatt ttccatataa
    30601 tattctccta tttctttata agaaaaatta tagcggaaat accatcaatt taaaagagat
    30661 atccagatat aaactaccat ttctctttac aggcaattta atgttgaaga tccgcttcgt
    30721 ttttacatca aactttgata taaagcaatg atttcttcta ctgtcgtttc tcttggattt
    30781 cctggtgtgc atacatctcg caaagcatct tctgcaattg ctggaatatc tgcttctttt
    30841 acacctaatt cagaaatggt ttctgggatt ccaaccgctt tgctcaaccg atcgatctca
    30901 ccacaagccg cttctcttac atcttctaaa gacatttcag ctgctccttt aatccctaga
    30961 acaagagcga tctcacggta cttttctcca gtatattctt tgttatattt catgacagtt
    31021 ggcaaaagtg ctgcacacgc tacgccatga ggaatgttgt accatgcact taacgggtga
    31081 gccatcccgt gtaccagtcc taaaccgaca tttgagaagc ccattcccgc aatatactgt
    31141 cctaatgcca ttttttctcg cccattttga tcgccatcta cagaagcttc caaagaatgg
    31201 ccgatgattt cgattgcttt taaatgcagc atatcagtca tttcccaagc gccttttgtg
    31261 ataaaccctt caatagcatg cgtcatagca tccatacctg ttgatgcagc caattttttc
    31321 ggcattccca tcatcatatc gctgtcgatg aacgcgacga ttgggatatc atgaggatca
    31381 acacaaacaa attttcggtg atttacttca tctgtgatga cgtagttgat cgttacttct
    31441 gctgctgttc cagaagtcgt tggtatagct aatataggta aacatggatt tttcgtatca
    31501 gcaacacctt ctaaactaat gacgtctgaa aactctggat tcgtcaaaat aatcccaatg
    31561 gcttttgccg tatcgattgg cgaacctcca ccgattgcga tcaaacagtc tgcctctgcc
    31621 tctttaacaa aagccactcc atcttttaca ttttggatcg ttggattagg tacgatcccg
    31681 tcgtataaag cataagcgat tgctgcttta tctagaagtt ctgttacttt tgttgctata
    31741 ccgtgctcga tcaatccttt gtcagtaatg acagctgctt tgccgaatcc tcgctgctga
    31801 aattcaggga cgatcgactc aatcgctcct tttccaaaat acgatgtttc atttaaaatc
    31861 attcgattac tcattttcta ttcctcctaa tttgtattat ttttcacaat aaaaacgaaa
    31921 gaaccccaag caaccggttg cccagttgct tgttgctaca gttcaaagac tggcatcaat
    31981 tcccaactga ctggagaggc atctggattt gtctccatga tatcttccat gtacttccac
    32041 catttttgat tgatagatgt atttgccatt ttttcccata acttttcatc ttcgatcact
    32101 aagtaaccaa ataacaaact tgtactcgga tcaagataga tcgaatagtc gatgaccccg
    32161 tgttcacgaa gtgctgtacg catttctggc caaagttcat catgtctttt tttgtattct
    32221 tcttgttttt ctgggtaaac tttcatgcaa aaagcttttt tcaccatgtc agacacctgc
    32281 tttcgtatga acttcctcca aataacctgc ttttggagtg acaccaaacg catctgctaa
    32341 atgccacagg ttttcgtccg tgattgtttg caagattggt ccttgcgctt tgacgtacgt
    32401 atagacttct gctgcctttt ctgctgtctc gatcaaacca aagacttcat ccatgtcttt
    32461 tcctgacccg tagattccat gttgtggcca taaaaccaat cgagtttctt tcattttttc
    32521 agctgttgct tccccaatct catttgttcc cggaaccatc cacggaatga tggaaattcc
    32581 ttctggaaaa acaactaagc attccgtaca catttgccat agcgtacgac taaaggaacg
    32641 ttcgtctaat tcatgcgtaa aactcatcgc taatagatga ctagcatggc aatgcataac
    32701 gatccgatta tctgggtcta cttctaaacg ggcgatatga ctcatgaaat gacttggcaa
    32761 ctcgcttgta ggaaggcatt cgttttccag cccccacaaa agttccagtg ttttgccatc
    32821 ttcagcaact cgaacgattc caagattctc ggcaggttcg ctcgctacat ttttaaaata
    32881 tcttcctgaa ccagttacaa taaaatagca tcctgctaaa cggctggcat caaaaatcat
    32941 cgagatgcgg cggatcactt tttcaggatc caagaacggt gtgatctctt cttcttttag
    33001 taaataagaa atattcccgc catttctttc atcccatccc aagcgataaa gattcgacgt
    33061 cgtttcgatc atttctttca cataaggcgc ttgtaaaata tctatttttt tcaatatagc
    33121 cacactccta ttttcttaat tccaacacgt ctttttgata ttcctggact tcttccagcc
    33181 aatccaaacc aactggaact tgatttttgt aacagaaata attccataca tcggcatacg
    33241 ggaaatcttt caattcttcc gtataagcca ttcttgaagt aaagtcaaaa gccagctcca
    33301 tttctttcaa tcgctcaact ggttccagca tagctttcag cagtgctttt tgtgtatttc
    33361 tcgtaccgat gacccaagca gcgatacggt tgatcgttgc atcaaagaaa tccagaccga
    33421 tatttgtttt ttccaacaat tcgttacgga ctaattcttt agcgatttcc tgcaattcat
    33481 catccatcaa aaccacatgg tctgaatccc aacgcactgg acgggaaaca tgcaacatga
    33541 ttcctttgct gaataaagct aatgaagata atttattgga aatcacttct gttggatgaa
    33601 aatgtccagc atctagacaa acaagtttgt cacgagtcaa cccgtaaccc atataaaatt
    33661 catgggaacc gactgtataa gcttcagcgc ctaaaccaaa tagtttgctt tctactgcat
    33721 cttgtacata ccgttcatct aactcttctg taaaaatttc atcgagtgat tccattaaac
    33781 gttcccgtgg ggtaaaacga tcaatcggat tatctttcat cccatcaggt acccaaaagt
    33841 tattgatact tgtttgccca gtttctttcc caaagtactc cgcgattttg cggcttcttt
    33901 tcccgtgttc gatccagtat tcacgaactt ctttatctgg atgagccaat gtaaagccgt
    33961 ctttcatcat tggatgcgaa aagaaagtcg gattgaagtc aagaccgatt tcttcttttt
    34021 ttgcccactc tacccatttt tcaaaatgtt tcggctcaat ttcatttaga tcgacagatt
    34081 cttctgtatc caaatagata gcatgaaggt tcaatttgtg tttgcccggt atcaacgaat
    34141 aagccttctc cagatcttgt ctaagctctt caggtgttcg tgcggctcct ggaaaattcc
    34201 ctgtcgccat gattccaccg gtcaattcgc catccggtgt caagaaacct ttgacatcat
    34261 ctccttgcca gcaatgcata gaaattttga tgtcctgtat cttttctagt acagcatctg
    34321 tatctacacc gatagttgca tatttttctt ttgcttgttc gtaatttctt tcaatcgtgt
    34381 tcatacgatt actcctttga ttttattcac gtaccattcc atcgtgtttt gcttattttc
    34441 atctgggtaa aaagtttcac atggaaacga ctgtttgatt acatttcttg cttgttctat
    34501 attcgaaaac tgacctgtac tgagcatttg catggcaaca ttcccgattg ctgtggcttc
    34561 actaggtcct gcttctacaa tgattccggc ataatctgct gtcaattgat tcagtagacg
    34621 attattcgat ccgcccccta cgatgtgcag cttttccaat ttcgttccag ttaagtgttc
    34681 tactttgatc agctcttgtg cgtaacaaag agcaagatta tcatacacac aacgagcgat
    34741 agctcccgat gagacaggga ctggctgctc tgtttcaaaa cagtattgct gaatctcttc
    34801 tatcatattt gggggattca aaaatcgagg gtcattgatt tccatcactt gctgcagcgg
    34861 tttttctttt gcagcaagct ctgccaattc cgcataactg tactcgtact cttgcattcg
    34921 ggcaacttcc tggatcaacc acatccccat gatgtttttc aaaaaacgca ccgtgttatg
    34981 ggcaccccat tcattcgtat aattttcaga aaaagcattg cgagaaacat tggcgatctt
    35041 tgtttcaatc cctaaaagcg accatgtccc actgctgata taaccccaac gttctccttg
    35101 acctggcgtt cctaatacag ctgacgccgt atcatgactt gctactgtga taaaggtcac
    35161 atctggcaaa tcatattttg aaaattgttc tttttgcaga tctcctagta tcgttcccgg
    35221 ctctgtcaat ggtggaaata attccttcga gacaccgatt ttttcaagaa gttctatttc
    35281 ccattctttt gtccctgcat tcagcagctg catagtagat gcattagttt tctccgtcac
    35341 catgttacca gtaaagacat accctaaata atcaggtatc agtaacaatt tatgtgcttt
    35401 tgccaaattt tctttttctt cagcaaacaa ttggaacaag gtattgaaag gctggatctg
    35461 aatccctgtc tgctcataca acttatctag agaatagatt tcagaaaact gttctactgc
    35521 tccattggta cgcttgtccc gataagcaat cggatcattt atccgatccc cttgttcatc
    35581 aatcagacag taatcgactg cccatgtatc gatccccgca aaaaggcgat caatccctgt
    35641 ctgctttaat ttttcaaatc caatcaaaat ctctttgata agataatcga tcttccagcg
    35701 atcgtgaccg tctttgttcc gaaagccgtt tttgaaccgg tgtacttctt ccaagatcaa
    35761 agaacctttt gcatccctcg tacttttcat tagtcgtccg cttgatgcac cgatatcgat
    35821 ggcaacaaat gttggattca tttctccacc tcttttactt tattgttgat tagcacttta
    35881 ttaataaagc gttatcattt attgacaatg ctatcataat acagaatctt atattttgct
    35941 tcacactatt ttggtatata attgcattat cttgacaagt tgggagtgtt tacatggatt
    36001 actgggaaaa agccaaccac tcatggtcgg aagactcaaa aagagtgatc ttgacgccta
    36061 cacaaagaag caggaattta tttttttata tccaagaaat cggttacttc aaagcaccaa
    36121 tcccttacta tactgaacga gcacatttac cttcttactt gataaagttt actttgtctg
    36181 gcaaaggttc gcttacatat aatgataatc agcaacagct aaaagctgga gacttatttt
    36241 ttattgactg ccaagaatat cagcgttatc aaacgatcag cgaagaacca tgggaaatgg
    36301 attggatcca tttgaatggt cagaacgtcc aatcatttta tcaggagttc acaaaagacg
    36361 gccataacac cttccattca acaggagatc catcagaaaa caagatccac catttgatca
    36421 cagaattgat ccacatgcaa caaggcacga atgcccgtac cgatttccag tcctctgtag
    36481 ctatccacga gttgctgaat gaactgattt tgcaaaagta tcagctagat ttcgaagaag
    36541 aggatattcc cgcctacgtt tacaaactga agcaatactt agacgaacat ttcaaagaaa
    36601 cgattacact tgatcaatta gaacaacaat tcttattgaa taaatatcag ctgagcaaag
    36661 atttttccaa atttatcggt tctccaccaa tcgattattt gatcagcaaa aaactttctt
    36721 acgctaaaga ccttcttcgg tatacagaat ttacggttcg aatgatttcc gaagaaatag
    36781 ggattgagaa ttttgcttac tttagccgcc tgttcaaagc aaaaacagga ctatcgccga
    36841 gagactttcg taaattcagc tgattcatat aatatgtacg tcattttttc aaaatatttt
    36901 gaaatatata gataaaaata aaataaccgc agtttctgct tctatgtaaa caacagcagc
    36961 tactgcggtt attttttagt gatcattctc attgttcatc ttcaaaatgt tcataatgaa
    37021 ccaactgttt ctgtgtatca cgtttcaaaa attctttagg cgttatgccg aactctctct
    37081 tgaatgcttt cagaaaatgc ggataagaag aaaaaccact tttactatat acagcactag
    37141 agctgcggta ttctcgcaac aagtgcttgg catagatcag ctttttcttc attacatact
    37201 gatgaaacgt aaatccagtg tactttttaa attctcgtgt gatatggtat ttgctgacaa
    37261 aaaactgctt ttccatttcc tctaaagaaa gcggatgaga taaatttttt gagatatatt
    37321 gtaacatttg ttccacacgc tcattaggaa ctacagcatt ctgttcttgc gcactctctt
    37381 ctttttgtac aacttgattg agaaaaatca agtattctac cagagcttgt tcatatttta
    37441 tgtcttcacc gtaaattttt ttatcaggag gatggtcgat aaatgataat ttttgcttca
    37501 atacttcctg ttctatttgc aatacacgac tcctcttctc tcccattggt tgaaaacaag
    37561 ccgatagatt agaccatggc gtggaacgac tttctagaaa agaaggggtg acaaagatat
    37621 agacacgctc gaatacatct gaagattggc ggacgatacg atgcaaatca ttggaatgga
    37681 tgagcagcac acttcctgct ttgatcgtaa attgtcgtcc gttcaaataa aaagtcgctt
    37741 ctccctttaa tgtagcatgg atctcataga aatcatgatg atgatagatc gttttatttt
    37801 ggctgacgac atctttgaca tgatagattt catactgatc agaaaccatc gtattgactt
    37861 gttcaccgct gattttcttt tggagcatgt gccgcacctc cttttgttca cttatgcaca
    37921 attttattat accacgtttt ttgtaatcaa aaagagctgc ttttatatac tcttataaaa
    37981 aagaagagac gtggcatctt ctagccccgc cccctccatt ctaaatatta gttaaccaac
    38041 gtcttcttta cattattcaa gaagacttct tccataacga atcccatcat cttttgtttc
    38101 ttcaagaacc acatacaggt acggacgatt cttcatgatg atttctttga tttttgtgta
    38161 ctcgatcaca ccgtctccca aatttaccgg cacgattttt tcctgttcga cacgaaaatc
    38221 tttcaaatga accgcacata tcttttcacc aaaacgttca aaagcttctt ctaccaattg
    38281 gacttgttct tgataatcac tacttgtgat caaattggtc ggatctaaaa tgattcctag
    38341 atagtccgaa tctactgctt ggatcagctg ctctaccctt gctaaagaat acaccggatg
    38401 attcaaacct ggttctatcc ccaccatcat ttggtatttt tctccagctt ttaccaagcg
    38461 acggataacg gacacagctt cagcaaatgc ttcatctgtg aaattttctt ccgtatattg
    38521 gatttccggc aaaacacatc cagtttccga agcaaccatc gacgcaccaa aggaagaagc
    38581 atagcgaaga tactgctcga actttgtcag caactcttgt cgtatcgtta aatctggatg
    38641 aatcatattg atatagcaac ttaaaattcc tacggtcact tcttgctttt ctaaaactcg
    38701 tttgacataa tttcccatcc cagaattgat ctcatttgcg ccagaaggta tttcaggaaa
    38761 cgaaagacct aaggcgagct gcagtgtatg aatcccttgt tcttttgttt tttctgcaag
    38821 atcttctatc gtttggactt cggaaagatc atgtcctcta attgtaatgt tcaacataac
    38881 tattctccta actcagcata ttgggtagcc acatgacgat ttgcgggaag taagtaatca
    38941 aaaacaagac gactaagata accccataaa aaggaagaag cggtttcagc attgcctctg
    39001 cttttacgcc tcctacactt gcaccgacat atagtcctgt acctactggc ggcgtgatgg
    39061 agcctacaca cagattcatg attgaaaaca acccgtaatg tacaggatca acccctacac
    39121 ttgtgacgat tggtaaaaat attggtgtaa aaatcaagat agcaggcgcc acatccataa
    39181 acatacccac cagtaataac acaagattga ctaataacag aataataaat ttattatccg
    39241 ttacgcccat aatcaacgaa ctaattgcct gaggaatacc ggtaaacgcc atggcgaaag
    39301 acatcatgga agaagtagcc aataagaaca tgatcgtccc tgacattgct actgattgaa
    39361 acaacatatt aggtatatct tttagtttta ccgttttata gtaaaacata gcaatcaaca
    39421 gggagtaaat gacagctatc gctgaagctt ctactgctgt aaatatcccc gtcaagatac
    39481 caccgataat gatgacaatc aatagaaggc taggaatcgc ctcttttatt atttttccag
    39541 catgagccat ctgtcctttt tcgacaacag ggtatttatt ctttttagca acgatgaatg
    39601 ctactagcat gattcctagc gcccacaaag cgcctacgag ataacctccc ataaacaaag
    39661 cagagatcga agcaccacca gcaacaagtg aatacatgat aaaagcagta cttggaggaa
    39721 tgaccatacc tgtcggagct gacgcgatat tgaccgctgt cgcaaacttc cggtcatatc
    39781 cttcttccac ttgctgaggg atcaacactc cgccaatcgc ggtagaagcg gcaatggccg
    39841 agctagagat ggaaccaaat aaagcattcc ctaacacatt cgtatgagcc aaagcccccg
    39901 gcacgcgccc gacgaacaac atcgcaaaat ccactagttt cttagctatc cctccattat
    39961 tcatgataat gcccgagaga acgaaaaacg gaatagcgat cagcgaaaaa ctattgatac
    40021 ttcccaccat tttttgagct gaactaaaag cagaaacatc aaacggtata actaacaata
    40081 aagtcatcat cgatgagaat gcaatactta tagcaatcgg catccctaat acaagcaaca
    40141 caacaaatac aaaaaataac actaatccgg cttctattgc cattttattc ctccattttt
    40201 atattttttc ttgaattgct ggcttgttgc ttaatgtatc gattagattg atcaggctat
    40261 agataatgat gagtaccccc gatacaggta ctgacagata aacatatccc ataggaatac
    40321 ttaaagaagg cgagatctgc cccattgtca atgtgacagc attccatccg ccatacacca
    40381 tgatcatcgc agcaaatacc agaaataaac tttgaacaac aacttctaaa attttacttt
    40441 ttttgccagt taatttttca cttaaaaccg ttacggctaa atgtgctttt tgtcccacaa
    40501 catacgcgga agacaacatg gcaaaccaga caagaccgaa tcgaacaagc tcttccgtga
    40561 ccgtatttgg atttcctaaa accgttctgg aaaatacttg ccataaaaca acagcgacca
    40621 tcccaacaag caaaacagct cccaggatct ctaatacctt gtttaaaatc attttcatca
    40681 cagctacgct ccttgctctt ggatcttttg ataaaggtct ttgtaggtag gatcgttttt
    40741 gaatatatca tgaagtgggg caactgcctc tcgaaacggt gtattatcta catcattgaa
    40801 ttggactcca tgttcttctt gtgctaaagc tttttcttct tcaattgctt ttgcaaaagc
    40861 ttcaatttca aaaaccgttg attctttcgc tgcttcttca atggcttttt cctgctcatc
    40921 agttagctgt tctttgatgg catcattcat gatgatgata tctggcacac gtgtatgctc
    40981 atcataggaa tagtatttag ccacaccacc atgtccagct gtatacaaga caaattcatt
    41041 gttttctgat ccgttgatca aattggattg aagagacgtg tacacttcat cacttcccat
    41101 aggtacgggc gaaccgccta aaagctctac cattttgata gcggtttcgc tttgcatcac
    41161 acggatcttc tttcccttca aatcttccgg cgtatggatc ggaccgtcta ccatataaaa
    41221 gctgcgctga ccagaatcat aataggtcaa tccggtaaat cctaaatttt tggtcgattg
    41281 gtagattggc tccattattt cttcattatc catcacctta aagaaatgtt cttcatcatt
    41341 gaatagataa ggaactgaaa aaatcgaata atctttagag aaactttcca acgctgaggc
    41401 acttactttt gtgaaatcta ctgctcctga ctgtgtcaat tcgatcagtt cacgttctcc
    41461 acccagttgt ccatctggga aatactggat ctgtacggaa ccatctgttt tttcttttac
    41521 taaacgccca aattcattca ttgcatcaat tactggctgg ctgttgcttg cataagcaaa
    41581 gcgcaaggta actatctcat ttacatctgc atctgcgaca gttgccacac tagaattatt
    41641 tccgcagccg cttagaccta gcagacctag gctaagcgaa cttgctgcaa acagcagtga
    41701 tgtttttttc atatgggttt cctttctttt aacaataaga gacgagcaat tctgtcaaac
    41761 aaagcaccgt taacgactgt ccataaggca ttgctgtgat tccgatattc cgataaaaat
    41821 ctaaactgtc ccccattcct gtcccgattg aaacgttttg aacttctcct ttttcatcga
    41881 tttgttcaag caatccttgg atagcacgat aagcgacttc tttatattct tgtggtaaat
    41941 aacgtttatg gaccgctttc aaaataccgt aagcaaatcc tgcagttgca gaagattcta
    42001 cgtaagaaga aggatcatcc aacaacgtat gccataaacc gctttcagac tgataagttt
    42061 ttaatgcacg cacttgtgct tcaagtgttt cgatcaataa tttgcgcaat gcatcttctt
    42121 tcgataattc taagatttca atgatctctg gaatagcaat cgtcaaccag cagtttccac
    42181 gagcccaaaa tgcttcggca tagttatgat ttccttcaaa cgtccagccg tgataccaca
    42241 atcctgttcg tttatccatc aagtatttga tatggatcat aaactggtaa cgagcttctt
    42301 ctaaatattc tgggcgattc aacagtttcc cgatttttgc caaaggaagc actgtcatca
    42361 tcaatgtatc gtcccacaac tgattcttat tttccggtcc atatgtggca tgctgtaacc
    42421 cgtcttcatt tgtccgaggc atgtcattca tgacccattc tgcccattgt tctaaataag
    42481 gcaaatagcg tgaatctttc gtgtcttcat acaagtaagc catagtcaat agcggtgcca
    42541 ttgtattgac gtttttgggc ggagcgcctt cttccatacg atcctcaaac cattcattga
    42601 ccacttgata agcgcgagga ttttttgtca tctggtaatt tttataaata ccatatagac
    42661 cgacaccttg tggccaattc cacactgtcc agcttttgtc atctactttc agtccatcaa
    42721 agtccaacaa aaattcacca ttatcgtctt tgatcgttgt caggttttca acgagcaggt
    42781 caattttttc tttcactaat tctttaggta caaaaatctc ttctttaata atcatcttta
    42841 cgccaccttt ttaaacgctt tcattttata tccttaatat agcagtaata ccgtccacta
    42901 taaatatcgc tttttgaaca aaaactgtct aaaattgacc taaatgttaa ataacaaaag
    42961 taactttcag ttacttggaa attaaaaaaa gaacttggag aaacaacgtt ttccaagttc
    43021 tttttcatct attttcttat cttttttcta cgctttcttt tgcttatccg ttgtaagcat
    43081 caatgatgat ctgtttcaat tcagagatca atggttcttt cggattagct gtcgtacatt
    43141 ggtcttcgta agctaattca gccatacggt ctacggttgt atctagtgtt tcttgtgtga
    43201 ctccttgagc ttttagactc atgttgatgc cgacagattt tcctaattca taaacagccg
    43261 ttgctaaagc ttcgactaat tcctctgttg tttcgccttt cagacctaag aattttgcga
    43321 tatctgcgta atctgtatct gcacggaaat agtcatattt agggaacatc gcatgttttt
    43381 gtggatcttt tgcattatag cggataatat gcggtaaaag aatcgcattt gtccgtccat
    43441 gagggatccc gtattccccg ccgattttat gtgcgactga gtgacaaata cctaggaatg
    43501 cgttagcaaa cgccatacca gccattgtag aagcgttgtg cattttttca cgtgattcca
    43561 tatcaggtgt ttttactgat ttttctaaat agtcaaagac taacttgatt gcttgtaggc
    43621 tcaatccacg tgtataatct gaagccatga ctgaaacata ggattcgatt gcatgtgtta
    43681 acacatccat cccggtatct gctgtgacag atgctggaac tgacatgacg aattgaggat
    43741 cgacgattgc gacatctggc gtcaatgcat aatctgctaa cggatatttc acatgtgttt
    43801 cactgtcagt gatgaccgca aatggcgtta cttcagaacc agtccctgat gttgtcggga
    43861 tacatacgaa ttgtgttttt tctggtttgt cgatcttata tgtccgttta cggatatcca
    43921 agaatttttg ttttgcacca aagaaagatg tgtctgggtg ttcatagaac atccacattc
    43981 ccttagctgc gtccattgca gaaccgcctc caagagcgat tactgtatct ggcttgaagt
    44041 ctgccatcag tttcgttcct gcgtatacgg tatttgtcga tgggttcggt tcaacatctg
    44101 agaagacttc gatcttcaca tcatttttac gtcgtgccaa tacttcacga actgtatcgc
    44161 agtaaccgaa ttggaccata ccagggtcac aaacgatcat gacacgttca acgttttcca
    44221 ttttctctaa gtacaataga gagttctttt caaagaagat ttttggcggt aatttgaacc
    44281 attgcatgtt atttctccgt ttcgctacag ttttgacatt gattaagttt acagcagaga
    44341 cgttacgaga aacagagttc ttaccataag aaccgcatcc caaagtcaat gacggaatca
    44401 tttcgttata gatatcaccg atgccgcctt ctgcagatgg tgtattcact aaaatacgac
    44461 aagctttcat tcgaagaccg aatttgacat gcaactcttc gttttctgaa tggataactg
    44521 ctgtatgtcc taatcctcca agattcaaca taccttcaca caaatcaaac gcatggtccg
    44581 tgttgttagc tttgatcatg gcaagaactg gagaaagttt ttctcttgat agcggataat
    44641 ctgggcctac gccttccaat tcagcaatca gcattttcgt tccttttggt acactgattc
    44701 ctgccaattt tgcgatttcc atcgcagaat gtccaacgat tgatggattg accgcatatt
    44761 ttccttcgtt catcactgca tcttctaatt tttgcaattc atctggtttt acgatataaa
    44821 cttggtgtgc ttggaattct gcttttactg ctgcatagat ttctttatcc acgatcacag
    44881 cttgttcaga agcacaaatc attccattat caaatgtttt cgaaacgatc aaatcgttca
    44941 ctgcacgttt aatttttgct gttttttcaa tataagctgg tacgttacct ggacctacac
    45001 ctaaagctgg ttttcctgta gagtaagcag acttgaccat tccagctcca cctgtcgcta
    45061 agacgatcgc gatacctgga tggttcatta gcatagaagt cgcttcgatc gaaggatgtt
    45121 cgatccattg gatacaattt tcaggagctc cggcagcgat agcagcatca cgtaccactc
    45181 gtgctgcttc tgcagaagat ttttgggcac ttggatggaa agcaaaaacg attggatttc
    45241 ttgttttcaa cgcgatcatt gatttgaaaa tcgttgtgga agtcggattc gttgttggag
    45301 ttactccgca aaccacaccg actggttcag caatttcaat caaccctttt tggacatctt
    45361 cattgatcac accaactgtt ttatcatgtt tgatattatg ccaaatatat tcggacgcat
    45421 acatattttt gattgcttta tcttcgtaaa caccgcggcc tgtttcctct acagccatct
    45481 tcgctaatgg catatgttga tccaaagctg ccattgccat ttcatgcacg atatggtcta
    45541 ctttttcttg atcaaagctt tccatttctt tcaatgctgc atttgctttt tctgccaaat
    45601 catcaatcat tgcttgtacg tcaattgtct tcgttttttc tttttctatt actttagcca
    45661 tcattatcct cctaatgggt catggttatt tgttaaataa ttcacatgta tatcatacaa
    45721 tatttatttt tctatgtaaa cacttttttg tgattttttt cacagttatt tttaaatata
    45781 aaattgcttt atttacaggt gtttgtatag aaaaatatat aaagttattt attattattt
    45841 atatgacaca cccgattatt caatattgag caatatatcc catgctctta tctctttctt
    45901 ttttgaataa aaaatgaggt aggacatctt tctgccctac ctcgtcttta aataagcttt
    45961 gatacttatt tttcttcctt ctcttctgtc tcttccagcg tttctgttgt ttcagttttt
    46021 acttcaactg tctcagctgg ttcttctgtt gtttcaacag tagtcgttgc atcatttgtt
    46081 acgacagtcc ctggtttgac cgttttgatt gctgcacgat cgtactcaag gaagatccct
    46141 tcgcagtcta aaacaactgt ccgtttgtca ttgtcgattt ctgaaacaac tccatgtaat
    46201 ccgccgatcg ttacgactga atcacctggt ttcatgctat ctaacaagtt ttgacgttct
    46261 tgctgttgtt tcttttgcga acgtgacata aaaaaccaca ttgctaatag cgcgataaac
    46321 atcacaagca ttggtaatgt tcccattttt ttcacctcac taattctgta tctacaaaaa
    46381 acactttagc agaaaaatgc tgataacact agttttttac ttaattttta aaagattttc
    46441 ctttagaagc tttttgcgtt ttctttattg aacccgtatt cttcaaaaaa ggcttctcta
    46501 aattcaagca aattatcatc catgattgct tgacgtactt gtttcattag attcaacaag
    46561 aagtataaat tgtggtaaga agtcaaacga atcccaaatg tttcatcaca tttgatcaaa
    46621 tggcggatat aggcacgtgt atagttttta catgtataac aatcacattt ctcatcaatt
    46681 ggacggaaat catgggcata tttcgcattt ttcactacta gacggccttg agaagtcata
    46741 catgtgccat ttcgagcgat tcgagtaggc aagacacagt caaacatgtc tactccacgg
    46801 ataacaccat cgatcaatga atcagcagca ccgacaccca tcaagtaacg tggtttgttt
    46861 tcggggatca acggtgtagt gaattctaat acacgattca tttctgattt aggttccccg
    46921 acagataacc cgccgattga gtaaccaggg aaatccatcc cgactagatc tttcgcgctt
    46981 tgacgacgaa gatcttcaaa ccctgctcct tggatgattc caaataatcc ttgacggtct
    47041 ggattagcat gagctttcaa tcctcgttct gcccatcgag acgtacgttc tactgatttt
    47101 ttcacgtagt cgtaactttc atcgaatggt ggacattcgt caaaactcat catgatatca
    47161 gagcccaatt tattttggat gttgattgct ttttctggtg ataaaaacat acgcgaaccg
    47221 ttcaagtgat ttttgaaatg cacgccttct tcttcgatat tccgcatatc acttaatgaa
    47281 aaaacttgaa agccgcctga atctgttaaa atcggttgat cccagttcat aaatttatgc
    47341 agtccgccag cttcttctac taagtcttca ccagggcgta gccataaatg atatgtatta
    47401 cttaaaataa cgccagcacc catttctttc aactcttctg gggacattgt ttttactgta
    47461 gctaatgttc ctactggcat gaacattggt gtggggaaag ttccgtgtgg cgtgatcaat
    47521 tcccctaaac gcgcgccagt atgtttttct ttcttgatta aacgatagcg aatggcaggt
    47581 tgcgtcattc atttccctac tttctatata aaatatcttg acaactccca ccatcataat
    47641 cgtttcaagt gctttttgca agttccctcg gcttccttct actttattta atattctttt
    47701 ttttatcctt tttttatgaa taacaaaaaa aagaaaaaca ttatgacaga aatatttttt
    47761 gatacatggt atattggtgt tattaaagaa aaggagggaa aaatcatgca atcttctgca
    47821 gaattaaaac gccaagcaaa agagagtcta aaaggacgtt ggggacaagc tgttttacta
    47881 aatctaatcc ctacattgat tggtattgct actattcttc ttattatgat tcctattatc
    47941 ttgtttgcca ccaccatata tgatggatca gcgagcgtct cagaagcgac aacttcagga
    48001 ggcggtagtt caggaggcgg tggcggaatt atttccagca tcattagcgc tttgttcatg
    48061 agcggtattt cttggactta tcttgatatt ttacgtggac aaaaagatca gatcgaacca
    48121 ttcaaagatg catttcgtgg attcaaaggt ttgtttatcg gcggagtgat cagcttagct
    48181 gtgctgatct cgatctttac aacattatgg ggtttgctat tagttattcc tggaatcatt
    48241 aaaagctatt cttattctca aagctatttt atttactacg atatcgtcca agaaacagga
    48301 caaacaccta aagtactgga tacgatcact gcaagtcgaa aattgatgga tggttataaa
    48361 ggacaacttt tctggttaga tctttcattt atcggctggc atatcttagc attagcaact
    48421 gctggtattg gttatctatg gctcagccca tacatttctg caacaaaagc agcattttat
    48481 gaacaattgc caaaagatat ttaaaagaga gtgtgacttg tgaccgaggg ggtggcagga
    48541 gaacaccgtt tatcgagagg cagtaaaaga aacattcagc ccattagcaa aaaggaggtg
    48601 tgacaacatt catgtcacac ctcctttttg tatagttcca gtaaaaagaa aatcaactta
    48661 gaaacagtta atcattcctc ttcttctgct tccggtaatc gatggaactt gatcagttca
    48721 ttcaccgcga ttggtacgat agataaaatc cctaccaaga accagtgatt caaactgatg
    48781 tgtactaggc cgaatagttc ttgcgtaaat ggcagcaaag caacagccgt tgtaatagct
    48841 aatgccagta acgccatttc taccaaagct ttattggtcg ctaaattgac tttgaagatc
    48901 gattggctgc tgcgtacatt gaacacatgc agaatcgatg aataagctaa gatcaagaat
    48961 gcaacagttt gaccgacatg ctgacttgcg tctacccatg ggctgatagt atcgacataa
    49021 ctacctaaat aataaccaaa caaggttacg atggtgaaga cccctgcatt tatggcaatt
    49081 ttttgcaata aacctcgagc aaagatccct tcatttttcg gaatgggtgg ctgatccatg
    49141 atgccgtgtt ccgctggctc tcttcctaaa gcaaatcctg gaagtccatc agcaactaca
    49201 ttgacaaaca gtagttgcac agctgtaaat ggtgctcccc atcctaacag catcgcaatc
    49261 aaaacaataa agatctccga aatattacag cttagcaaaa agttgatagc tttacgaata
    49321 ttctgataaa ccgaacgtcc ttgactgaca gcgtctacga tcgtcgcaaa gttgtcatcc
    49381 gtcaaaatca tatctgcggc actttgcgcc acatctgttc ctgtgatccc catggcacac
    49441 ccaacgttac ttgctttcaa cgctggtgca tcattcacac catccccagt catcgctacg
    49501 actgcacccg tacgttgcca agatttgacg atccggatct tgtcttctgg cgttacccga
    49561 gcatagacag agagagcttt gacacgcgca tctaattctt cgtctgataa ttgatgcagt
    49621 tctgatcctg aaagtgcttc tgacggatca ttcaagatac ctaattcttt cgctatggca
    49681 ctggcagtca ccacatgatc tccagtgatc atgactgttt tgatgccagc ctttttcgct
    49741 cgcttgattg ctcctttact ttctggacgt ggtggatcga tcattccgat cagaccaatc
    49801 aaacgtaagt ctttttccag tgcttcagaa gaaatatctt gcggttcttc gtcataagta
    49861 gcataaccaa cagcaatcac tctcagtgcc cgtttaccaa agcgatcatt aacgatcgcc
    49921 gcttgttcta catcgcctga actaaagcgc ggagcaagaa catcgaatgc tcctttggtg
    49981 attgagatgt atttttcgcc catttggtga actgttgtca tcatttttcg ttcagaatca
    50041 aaaggaagct cgtttactcg tggatatttt tcttctaatt ctgcctttgt atgataattc
    50101 tcttcaacag cccttacgat ggctacttct gttggattac ctttgataac aaggtcgtca
    50161 tcttcttgct cgaccgtcac atctgtacat agtgcagcca ttttcaatat ttccatcgcg
    50221 ctgttcgtca ttgaatcttc gatattcgtt acttcatctt ctttgctcca tacccggcgg
    50281 acgcgcattt cattttgtgt cagcgttcct gttttgtctg agcagataac attagcggtt
    50341 cctagcgttt caactgctgg caactgtcgg atgatcgcat gtttcctcgc catcttctgc
    50401 acaccgtatg caagtgtcaa cgtaacaata acagtcaagg tttccggtac agctgcaaca
    50461 gccaaggaaa tcgaagtcat gaacatctca aggagtggtt ctccttgcag ttcaccaatc
    50521 acaaatacta aagccgcagc cgctaaagcg atcatactga ttcgtttgcc tagctgattc
    50581 aatcggacct gtaaaggtgt tttttgcata gtattttcgt tcaaaagatc cgcgatctta
    50641 cccatttctg tagccatgcc aatggcagtg acgatcgctt tcccacgacc tgcagcaacc
    50701 gtacatcctt taaataccat attaagttgg tcacctaatg actcatcttc ttcagcaata
    50761 aatgctgcat ctttttcaac tgcttcactt tctccagtca acgccgattc ttctacacgt
    50821 aattgtgatg cttcgatcaa acgggcatcg gcagaaatag catcaccgga ctctaaaaca
    50881 agaatatcac ccactaccag ttgctctgac tcaacagtgg tcactttgcc gtctcgtatg
    50941 acagttgccg tttgtttatt catatcttgc aaggattcca gtgctttttc tgcgtttcct
    51001 tcttggacaa tcgccaatac cgagttgatc acaacgatag cgatgatcag cagtccttca
    51061 aataaatccc catgttctgt agcaaatgcg gtataaaagg aaatcaccgc tgctacaaga
    51121 aggatgatcg ttgtaaaatc ggacaagctt cgaaagaatt ttttgatcat cgattctttc
    51181 ggcgcctcat cgaatttatt caatccctgc ttttttcgcc gttcatccac ttcttgtgaa
    51241 gacaacccag tatctggatc tgcttctgtt accttcttta cttcttctat tgtctgcttg
    51301 tattcttcca tcctattcct cctttaaaat cttcgtacaa aaaaaggtac ttattttact
    51361 ataacatcct ttttttattt acttcaaata aagggcttat cgattatttg ttaagaaagc
    51421 gtaaaattta aagtgcttat atttgcaaac gctaccaaag gatgctatta tatagatgta
    51481 aggaaggagg ataccatcaa atagacagaa gaatttctcg atagaacagt gtaagcaaac
    51541 aaagttgtta gaagtacagg tttcacccag acatgcgtgt agagtacaaa cctataaata
    51601 gtgaatgcgt atggatgttt ggatcacgaa gtatccgaaa caagaagtaa tgcgatacgt
    51661 aacaattgaa taacgtaaac gtctgaaaat tgaataggaa caagagatgt ttcaaatgtt
    51721 gaagatgtaa cgaaaacaaa atttttgttt aacatgaaga aattcaacac cgtgctttgc
    51781 atgtatgatg gaaagttaca atcatttctg tttatttgaa tccttccgag gtacaagaaa
    51841 gccgagctta tcgagagctc ggctttttct ttgtcataaa atctttgtaa agcttttttg
    51901 ttattcaaaa ctagcggctt ttcataacct tatttcacga acatcgcatc tccaaaacta
    51961 aagaaacgat agcgctcttc aatcgcatgc tggtaagcag ataaagtcaa ttctcttcct
    52021 gcaaaggcac taactaacat gactaaagtc gattttggca aatggaaatt agttgagaaa
    52081 gcttctacca ctttgaattc atagccaggc gtaatgaaaa tatcagtcca tccgctgtct
    52141 gctttgattt ctccattaaa tttcgttcca atcgtttcta atgttcgaat tgaagttgtt
    52201 ccgacagcca cgattttccc gccattttta cgtacttggt tcaatcgctc agccgcttct
    52261 tccgtcaaac ggtagaattc actatgcata tggtgttctt caattttatc tacgctgaca
    52321 ggtcggaatg tacctaaccc tacatgtagt gtcagataga ctaattcaac accctttgct
    52381 tcgatctcat caagtaattc tttcgtaaaa tgcaatccag ctgttggcgc tgcagcagaa
    52441 ccattttctt tcgcatagac agtttgatag cgatctgggt cttccagacg ttctttgata
    52501 tatggaggca atggcatttc tcccaatgat tcaagaatct ctagaaaaat tccttcatac
    52561 tcaaagtcaa tgatccgtcc gccgtgttcc aattcttctt tcactactgc ttgcaatctg
    52621 ccatcaccaa aagagattct tgtcccaact ttggcacgtt tcgcaggttt gatcaatgtc
    52681 tcccacgtat ctccttgtgt attcgttaga agaaggactt ctaaatgcgc accagtatct
    52741 ggtttttctc catataaacg agcaggcaga actcgtgtat tattcataac tagcgcatca
    52801 ccaggctgta actcttcaat gatttcatgg aaatgtttat cttccatctc tccagttttc
    52861 cgatccaaaa caagtaaacg agaatgatcg cggtctttta atggtgtttg tgcgattaat
    52921 tcttccggca agtcaaaatc aaaatcttct gttgttaaca tatcgtcact cactttctat
    52981 catgtgcttc atcattctat catgtttttc gttctgttgg caaaatcagc actttttcac
    53041 ttctattttt tgttttccta aataaaaaaa ctaccctatt tgtatttttt cattgtttta
    53101 ggcatactag taatagatag ttcttcattg aaaatagaaa aagaggtggc accatgattg
    53161 aatttcaaaa tgtgtcaaaa atttataaag gtggaaaagt cgctgtagag gacgtcaatc
    53221 tttcatttga aaagggtgag ttcatttgtt ttatcggtac gagcggaagc ggaaaaacga
    53281 cgacgatgcg gatgatcaac cgaatgactg atccaacaca agggaaaatt ttgattgatg
    53341 gaaaatccat ccaagaaatt gatccagtgg aattgcgacg tcagatcggc tatgttatcc
    53401 aaagtattgg attgatgcct cacatgacga tccgtgaaaa tatcactctt gtccaaaaat
    53461 tgttgaaagt cagtcaagaa gaacggaata aaacagctga aaaaatgatt gatcttgtgg
    53521 aattgccccg tgagatgtta gatcgctacc cacatgaatt atctggtggt cagcagcagc
    53581 gtattggagt cgttcgtgcc cttgcagcga atcaagatat catcttgatg gacgagccat
    53641 tcggtgcgct tgatccaatc actcgtgatt ccttgcaaga ccttgtcaaa gatttacaag
    53701 agcgtttggg aaaaacgatc gtatttgtaa cccatgacat ggatgaagcg attaaactag
    53761 caagccgtat cgccatcatg agtgaaggcc gtgtgatcaa tttgatacac cagaaaatat
    53821 tttacgtcat cctgcaaacg atttcgtaga agagttgatt ggagaagatc gcctactaca
    53881 agccaagcca aatgcgacaa gagtcggcga agttatgcta aatacagcaa tcacgattac
    53941 accggaaaaa tcattacaag aagcaatcaa actgatgagg gaaaaacgtg tagatacgtt
    54001 gttagttacc gataattcga atgtattaaa aggatttatc gatgtagaaa cgttgaataa
    54061 aaaacgagga aaagtatcca gtgtcggcga cattttgaat aaacatgttt tttacgtaaa
    54121 agaatccgcc tatcttcgtg ataccttgca acgcatttta aaacgcgggc tgaaatacgt
    54181 gccggtcgtt gatgaacaga acaaagtcgt aggtatcctg actcgtgcat cattagtgga
    54241 tatcgtctac gatgtgattt ggggagaaga tcccgcaact actgatgttg cagagtcagc
    54301 tactcctgaa gaatctaagg aggtctgacc tatgcagcag ttttttcatc agcacggttc
    54361 agaaattctc tcaaaaggtt ttgaacatct ttatatttca ggacttgctt tgatcattgg
    54421 tatcatcatt gctgtaccgc ttggtgtctt actgacacgt tttccaaaag ctgccacgat
    54481 tgtgattggc ttaaccagtg ccttgcagac aatcccttct cttgctttat tggcattgat
    54541 gatcccgcta tttggcgtag gtaaattacc tgcgattatc gcactcttta tttattcatt
    54601 attgccgatt ttacgtaata cgtacattgg gatgaaaaat gtcgattgga attatcggga
    54661 tgtggcaaaa ggcatgggga tgactgagtt ccaatccatt ttttccgttg aattgcccat
    54721 tgctatgcca acgatcatgg ctggtattcg tttagctgcc gtttacgtga ttgcatgggc
    54781 tacacttgcc tcttatatcg gcgctggtgg tttaggcgat ttgattttta gtggactgaa
    54841 taattaccaa ccagatctca ttttggcagg tacgatccct gtaacaattt tagcactatt
    54901 ggctgattgg cttctaggtc tcttggaaca ccggctgaca ccgatcgcac ttagagagga
    54961 gaatgaagaa tgaaacgtat actaaaaatg accatggttc ttttctctct tcttatactt
    55021 gcgagttgtt ccttccctgg tcttgccagc aatacagatg aagacaccat ttcgatcaca
    55081 ggtggaatca cttcagaagc acagatctta gcaagtctag ttgccggaat ggttgaacat
    55141 tatacagata aaaataccgc aatcatcaac aatctggcta ctacaactat caaccatcaa
    55201 gcgatgatga acggagatgc ttcaatttca gcggctcgct acacaggaac agatttaacg
    55261 actacattga accttcctcc tgaaaaagat ccgaaaaaag cgtttgctac cgtgaaagat
    55321 gaatttgaga aaagatacgg tcaaacgtgg ttcccgtcct atagttttga aaatacgtat
    55381 gtctttcttg ttcgaaaaga caccgcacaa aaataccatc tatctaaagt cagcgatctg
    55441 aaaaatgtag cggatgaatt agtagctggt gtagatactt cttggatcaa tcgtaaagga
    55501 gacggttatg acggcttcca agaaacttat ggcttctcct tcaattcgat attacctatg
    55561 cagattggac ttgtttatga cgcagtcgaa gcaggaaaaa tggatattgt tcttggctac
    55621 tctacggatg gacggatcgc cagttatgat ctagtcatgt tggaagatga tcgtcaattc
    55681 ttcccaccat atgatgctgc tccagttgta gacaacaaga tattagctga tacgcctcat
    55741 ttaaaagaag ccttaaataa gttaggtcat accatcagta cggaaaaaat gcaggaattg
    55801 aactacgaag cagataataa tctagtggaa ccatcagtag tagcacaacg tttcttacaa
    55861 gaaaaccact attttgaagg gaagtgaagc aattggataa aatgaatgtc tttcaacaat
    55921 ttttctatta tttccaggaa aatggctcct atatctttgc acaatttatc cgtcacttcc
    55981 tgatttctat ctatggtgtc ctctttgcag cagttgtcgg gattccggtt gggatcatga
    56041 tttctaggag acgtaaatta gcaaactggg tgattcgttt ggcgaatatc atccagacga
    56101 tcccttctct agccatgatc tccattttaa tcattggatt aggcctggga gtcaatgtcg
    56161 tgattgttac tgtatttttg tattccctct taccgatcat caagaatacg tataccggga
    56221 tgatccaagt agacaaaaac atccttgatg tcggcaaagg catggggatg actgctagac
    56281 aacgactatt catggtagag ttaccactct ctgtttctgt tatcatggca ggcataagaa
    56341 acgcgttagt tgttgccatc gggataaccg cgatcggtgc attcgtcggt gctggtggtc
    56401 ttggagatat cattatccga ggaaccaatg caacagaggg cacatcgatt attttagcag
    56461 gtgcattgcc aactgcatta atggcgatca tcacggactg gcttttaggt attttggaaa
    56521 gacggctaga tccagccagc cgtacatcac gctgatgtta ttgtaaaaaa caaaagcttc
    56581 tgcgaccatt tgttggtcgc aaaggctttt ttagttctgt catttttaag gcaataatcc
    56641 actctgattc ttccctcctt atgggcaaag agttgctttt cttagctatt tatccattcc
    56701 gtatactcca acttcatact gccaacgggt tcatatctgc atccaccgac taagtttttt
    56761 tcttatccgc taaggcattt tgccaatcca ttttcccatc actcgtcatg gaattcatac
    56821 cacatgatct ctaaatactc gtctgcttgt tctttgaatt gggcatcaat tcttacatcc
    56881 tttggcgtgg ctttcattta agccttttcc atcagtcctc gattgttcga tgccttcagc
    56941 tgcatcatca aaactggtga ctgccgtatc ggtaacttcg atcatctttt atacaatcgg
    57001 gaacttgcca tcctcatcct tggctaaaaa catatctagc cattccagct tctcgccatg
    57061 ggaggtttgc ggttcctaaa accggtacac gattcttatg gactgcatcc gtcgcaacta
    57121 gacttggcga tacgatgatc cttcaccaga agagcacccc cgtaaaccaa ttgatcgata
    57181 tattgccaat aagaaaagac attggcgtaa aagatatcga tccctcgtga tgcattgccg
    57241 ttgatactgc tattcatggc cgaaagcgcc acgactttaa cttcttccgt ttgagtgaca
    57301 ttcatgttcg gcaatgtcgc tttggatacc cgctctgcta aaggaacgac actggtattg
    57361 tatttcgtat ctgtatcttt tccaaaagac catgccaacg aatcctctgg gaaccaataa
    57421 gacgactccg gttgattgtc cattgctttt ttcaacatac ttttggctgt ttctcgataa
    57481 acgacatttc caattgtctt cgcctcactt tggcgataat cgatggtcgt tgccactgcc
    57541 aacactaaca acgatgctcc aattttactg attttccttg tcacacttct tcctccttgt
    57601 tttacataaa cccgtcaaca ttccattcct aaactcaaac aatgaccctt caaaggtgga
    57661 gatataggaa caaattaaag aaatcatgca ataaatccaa gaaggttggt tttagaacaa
    57721 aaaaagagtc tcctttttgg ggagactctt tgttaactat ctaaaaaggt tggctcttct
    57781 cacttcactc ataatgtgtc caggcagaat aaatttctac aactttattt tcctcatcaa
    57841 ctttgtaaac cactcgatgc tgtctattca gcctgcgtga gtatctgcct tcatgtgtag
    57901 gtctcagctt ttcaaaagac tgcgtgggca tatatggatc ttccttcaat gtctgaatca
    57961 cttcttcaaa ttgtttcttt aaattcgttt gcttgatttt tcttaaatcc actttcgcag
    58021 agtttttgat tgcgactgta tagttactca tcaaagagct tcccaatcaa tatcatcgat
    58081 attcgtaaag ctactgtcgt ctttttcgcg gtctcgtact ttatctaaag tacctgtttg
    58141 ctcaagaaaa agggtttctt gaatcgctct ccagtcttcc agcccaatca atacagcatt
    58201 gtttccacta cgatcactaa taatctcgat ttcagtatga ttttcattta cctctttcaa
    58261 taactgataa aaatttttac gagcagtgct aggattcatc gttgtattag ccattgtctc
    58321 tcctcctttt tctgatataa ttatagcgta cttgattaag tacgtcaaaa aaatacaata
    58381 aagcacttaa attcagcaaa cttttcctct tttttacata gagaaaaaga ccccttttta
    58441 gggaggatct ttgtccacgc tcacttctta ctcctttacg actagcggta gccaaacgta
    58501 aagtaattgg taacaacgta tcatctttag caaaggtcga atccaaagcc tgtgagtcta
    58561 tgcaacaaca ccaatacacg aaggttcctc gttgcagtgt gacttgattg atatcttgaa
    58621 taacctgagg atacgcacga aagatttgta acggttattc aaaactgatt ttggccgtca
    58681 agtcttctac tgcctcaaaa atgattgcct ctttcgtctc ctgtcattca tgttcactat
    58741 cgatttgata cttgcttaga ccgttgggtt tgtgaatcac caattaaaaa tggcactgag
    58801 ttatggtaac aaaaaatggt cacttgacga ggctgagatt cctacttatg aagacacatg
    58861 gcacttaaga gtcgaggtaa aggaagaagc tgaaaatata ggaggctgta cattatttgg
    58921 caaaggattc ggaaattatg atcaagacat caaaatcaaa atgttttaca gctaaatagg
    58981 aagaataata acatttcata cgctgttccc atctttaatg gcttatagag ggcaccttta
    59041 gctgagaata agacaaaaca gaccctctag cttcaattaa gagctagagg gtctgttttt
    59101 gattttaaaa cttggtttat ttcttcaaat ggtagctata ggtgctgaca acgatatttt
    59161 cacgccaaga caatcgcaat cttaaccgca aacttgtttg gttatggaga atattctgcc
    59221 agcgtctgtt tggtacaaat tgaggaatca acacagtagt tgtatagttt tgtttcgctg
    59281 catttttact tacgatatct acataacgga taattggatt ttcaatcgag cgataagaag
    59341 agtggacgat tgaaaaacgg acatctggga aatgcttttt aaattctgct tcgatttctt
    59401 tttctttttc gacatcttcg tctagcgaaa catgcatcgc aacgacataa tcaccgatcg
    59461 aacgtgcgta attcaacgca ccgatattta cccgtgtcac attcccgact aatacaagaa
    59521 cggtattccc gtcataatca tgtagttttg catcattttc taaacgaagc tgttctgcta
    59581 cttttttata atgatcatgg atcttataaa aaatgaacat aacgattggc ataatgatga
    59641 agaaaggcca aatgtcccct aaccgataga caaacaaaat cgcaatgatt gcatatgaga
    59701 tgaacgcacc cgtaatattg gcaatcgatt tgcttaacca tctttttcct tcttttttcc
    59761 atttgacgac catccctgtt tgagataaag caaatggaat gaacacgccg atagagtata
    59821 acggaatcaa gcgttctgtt gagccatgga aaataaacag caatacgatc gaacctagcg
    59881 ctagtgtgat aatcccattg gaatagccta gtcgatcccc acgatcttga tacatgtgag
    59941 gcatgaattt atcttttgcc agattgtatg caagaactgg aaaagcagaa aaaccggtgt
    60001 tagcagcaac tgctaaaatc agcgctgtag caaattgaag gacgtaatac aaaataccat
    60061 gtccaaagac agctttacca atttgagaca aaacagtgac ttctttttca ggaacgatcc
    60121 cataccaata gttgataaat gtgatcccca cgaagaagaa acctaagatc aatgccatca
    60181 tcgtcaatgt agcagcggca ttttttgctc ttggcttctt aaagaatggt acagcattac
    60241 taatcgcctc caccccagtt aaagaggaag aaccagaaga aaatgctcgt aaaatcaaag
    60301 cgatagaaac acccggcaca gccgctcctg gaagtgcagt agcttgcaat ggcacagcac
    60361 ctgtcacgat attatataaa ccaacaacga tcagtaacga gatcacaagg ataaatgtgt
    60421 acaccggaaa taataggaac gatgcagatt ccctcagccc acgaagattc agcatcatca
    60481 gtagcaaaac gattgtcaca gaaatagcga cttgatgacc gtagagtgct ggcaccgctg
    60541 atgtgatcgc ttcagcacct gctgagacag atactgctac tgtcaacatg tagtcgatca
    60601 ataaagagcc accggaaatc aagccagcat tctttccaag attctcactg ctgacgacat
    60661 atgctccccc gccatgagga taagcatgga tgatttgacg atatgatagc gttaatgaaa
    60721 tcaataaaat aataacaaaa gcggcaatgg gtagcgaata ccagatggct gcagcagaca
    60781 aagcgactaa aacgacaacg atttgttccg taccatatgc aatagatgat aacgcatcag
    60841 acgacaataa tgccaaagca gcaaatcggg ttaatttgtg ctcatcattt tcagcagatt
    60901 ttaaaggctt acctactaac aaccttttta agtaatccaa tttctaaact cctttacaca
    60961 caattttact tcaagtttta tagtcgaaaa tttatagtaa acaagatgtt atagtacgct
    61021 cactgacctt aaaagtcaat aaactttaag aaaataattg gaaaaaaaca gaaaaatatc
    61081 aagcgctttt tcaaacgctt taatacagca aaaatctaac atttttttat gggctttttc
    61141 tttgaattct ataataaaag gatagaactt gacttagaaa atcaagttct atcctttttg
    61201 atatcttaac ttttggaaga gagatgtaag gctgctttct acccatcatt cttactactc
    61261 gaagcatagc gcagttgtca caacctcttg ataaacggtg tttaaaagct tgcatctgcg
    61321 gtaataagcc caaccaaatt aaaaacataa gaagctgttt ttatttggtc gttcttatta
    61381 cttgatgcag agcagctttt ttacaacctc ttggtacacg gtgttcaatc agctgaccct
    61441 cggtattagc tcaaaaaaac ggcaaaatat gaggaactat ttttccattt ttttctctaa
    61501 tactcaggtc aaaacactga tttcacaacc tcttttttta atgaaaaata tggctctcat
    61561 gcgccgcttc tttgaatttc gtcatttttt cttcgatgaa cggatagatt gccgttccta
    61621 cgattccaaa gacaacaaac aaaccgatca tgatccagat tgctttcgta gcatttggcc
    61681 aataaattcc tcctgcagat tcccgtaaca agtttaccgc atgagtaaat ggcaagaacg
    61741 gattgatcat ttggaagaat ttccctgaga cttggatcgg atagttcccg cctccgccag
    61801 agatcgacag tactaagatg ataatagcga tccctttacc gacattatcg aataatgcag
    61861 ccaagacata gacgatcatc atgaacgcta aggcgatcaa gacagcaaat gacacgctgt
    61921 agaccggatt ttggacatct actcctagta agaaaatatt ccctaatgta acgatcaagc
    61981 tttgcgcaat cgccattgtc aaaaatgtca gcatacgagc aacgaattgt tctcgtttcg
    62041 taaaattgtt tcggtctttc tttcctaaat agtattctgt cgtagcaaca cttgaaagaa
    62101 gaacagcgcc gacccacaga catagtgcag tataaaacgg ggtgctagct gaaccgttat
    62161 ttgcgattgg ataaagcgca ttggtttgta gctcaacagg ttgtgtaaag aagtcacttt
    62221 cttttgttgc gtctagtttc aataatttga tgacttcgcc tagatcgact tcttgttcgc
    62281 cttttcggat cgcgtctgct gctttttgga taccagcttt gatatttggc cagtcattgt
    62341 tgatcaagtc cgcagcagcg ttcacagctg tttcgacttc tggcattttt tcatttactg
    62401 ttgtcaatgt accggtgatt tcatttttta gtgctggcca atcattttgg ataaaatcag
    62461 cggccaatcc taatttttgc tgaatgatcg gtaattcatt gttgtatagg tctgcccctt
    62521 cattgatgcc attgacgatg gtatccatat ttccattcag cattgtattg gcatcatgga
    62581 tttcttgtcc aattgcaggc atttcttttt gatatttttc taaaattgcc actgcattcg
    62641 ttacagttgc tttcgttgag ctgagaagcg aatcaaagtc gatctggtcc gctttgctca
    62701 gtgcgccttg tgcagtccca atcgtatcga tgacttggtc tagtagagaa gtgacagtgg
    62761 aactgatttg acctgtattg ataccagcaa tgatgccatt gatattatta acaacggcat
    62821 caatttgatc tagcaattca tcaatagcct tcgtgtcttc attttgaaca gctgtgttca
    62881 aagcagatag gcggttttgc agattgctta gcaagggttt taattgattt agctgatcaa
    62941 taattggctg taatttcgta tttccagcac ttgtttgcaa tttagtcagg aactcgacca
    63001 gtttatcgat tgcattttgt tgttgagata ttccgtcaat aaagccgttc aatacattgc
    63061 tgatgttttt cttttcatca tcagtcagct taccatcttc aatagctgtt cttacagtat
    63121 ctattaacga acctgtcgtt gttgccactt gtccaattga cttcaatgta acatctacac
    63181 tgtttgtgat gcttggtatc gcatctttca gctgagaggc acctgatttt gtttggtttg
    63241 ctagatcact tgcctgattt tctaaatttt tcacatctgg caagatatct tgtacttgtt
    63301 ggatgatcgt caagccttgt tttgcttcat cgattccttg gttcattgtc tcttggatcg
    63361 aagcaaaatc gccgtcgatc tctgctagtt gcttaccggc attttggatc tctgggattt
    63421 tttcttgcaa atcaaggatg atttttgctt gatctttcaa ctcaggcatt ttgtcattca
    63481 atgcaacgac tttttcaccc atctcatcta ctttagggat gtaatccacg aattcatttg
    63541 ctttattcag tttttccttg atttcaggta gttgactttg taattcgagg acttgttttg
    63601 tgtagccgtc gatcgtgtcc aaattttcat ctgttgataa aatcatatct ttaactttat
    63661 tgatacttac taggttcgta tcgatgtcgt aacctatctc attgaatact ttcaataaag
    63721 tcgaactggc tgttttgata aattcatttg tgatttgcga ttggatcgaa cttgctcctt
    63781 tatcggtgat tttcggtgca atcgcattga ttttttcatt taccgtatat tcgatttttg
    63841 gtttcttgat atcaccacta gtaaaactca gcagatcttc tgagaaatcc tttggcaagt
    63901 aaatacctgc gtaatacttt ccggaacgga ctccgtcttc taattgttct tttgaatcaa
    63961 cgaattgcca tcctaattgt ttatttttat gaagcgattc gatgacttgc tccccaatgg
    64021 ccacttcttt accttgaaac tctgctggct tgtctgcact gtaaacagca attggcagct
    64081 ctccagtatt tccatatgga tcccataatg ctttgatgtt aaaccacgca tatagcgaag
    64141 ggatgatcat gatcgcgatg atcagaagtg ttgcaatcgg gtttttgaaa atccgtttcc
    64201 agtcaagcaa atataatcga agcgtatttt tgattgactg catagttctc ccccttcttc
    64261 ttttttcagc tgaattactt taccatgtat ttcatacttt tttcaatcgg cttttttcaa
    64321 aaaagtcgaa tttttggaca tatcacggca attgtctcat tgtaatctct tacatattga
    64381 gaaataatta aggattacag cctttacttt atcatgaaat aaaagggaaa gataatttta
    64441 tgccaaagtt tttatctatt atacgtatac gcaaaaaagc taaaacattt tttgtctttg
    64501 cccctacaaa aatgtttcag cttttttcgt tttttattcc tgtatttctt atttttcgaa
    64561 tgacaggatg attttcccag caggaggatg tccttcaact agctgatgtg cttggatgac
    64621 atgcttaagt gaagcagaca gattcattga aataaaaaca tgaaaggctc ctttttggta
    64681 ggctttgatt atgccagcaa aggcttctgc atctagatat tcctttcttg gaacaaacga
    64741 ttcataaaag ccttcctttt tttgccgcaa atctagatct gggaggtcat ttaatgcaac
    64801 atacctgccg cctggtttca tgatctggat gccggtttct cctttgatac ttcctttcgt
    64861 cgcatcgatt acaaggtctg cctgatcagc aaattgcaga cctggatttg ttttatcata
    64921 agcagcaaat gctgaagctc ctaatttttt taccttttcc tcatttttac tggatgcact
    64981 ggttaagatt cggattccct tttcatggag aagctggatc agacttgacc cgaccgcacc
    65041 tgatgctcct agtatcatta cagtatctgt cggctggatt tccgtcaaat ggttgattag
    65101 attataagcc gtaattcctg gcgtgaccat tccggctgct tcttcccaac tcattttatc
    65161 tggaattttt gctacttttg ctgatggcaa cacgacttct tctccgtaag tcccgccgac
    65221 tgcatggaca gctactcgat cacctacatg aacagttgtg acatctgaac caatctctgt
    65281 cacaatacca gcgccatcat tacctggtac ataagggaat ttcaatgttc gtatctcttt
    65341 catttctcct aaacgcaagg caacatcata aggattcaca ctgaatgctt ttatttctac
    65401 acgaacatgt ccagaaccga cttctctggg atgagcatcg atttcttcaa aaacatcttg
    65461 tgcttctccg taaccgttaa ttgcaaatct tcgcatggtc accatctccc tttttcttta
    65521 agtttacgtt tatgccttta ttttgacaaa aaaaatgtct tttcgttttg gcgcatgatt
    65581 caaaacaaaa tagttgtctt ttttcgcgta acgtgttaaa ttatttatct aaaagcgtaa
    65641 agattacgat ttacgctaat aaaaaataat tggaggaatt tgttgatggc taaaaaatca
    65701 aagattgcca aagcaaaaaa acagcaagaa acgattgaac gctatgctgc tttacgccag
    65761 gaattaaaag cgcaaaaaga ttacgaaggc ttgcgtaaac ttcctttaga tgctcgtcca
    65821 gaacgattga aaaatcgtga tttaatcgat ggacgccctc gcggatacat gcgtaaattc
    65881 gggatgtccc gtataaaatt ccgtgaatta gcacataaag gtttgattcc aggagtaaaa
    65941 aaagccagct ggtaatccaa gtctggctgt aatgtaaagg aggagggcgc atggaccact
    66001 ccttggatca tcaaaaaaga tacgttcgct tcttctagca gaaatgctgg tactagaagc
    66061 agacgtatct ttttttacgc acagctggtc cttttttata tgtaaaggct gcatggttac
    66121 ttcgctatat ttatttagta acaggagtca gtgcttcgat acccacacta attttcaatg
    66181 ctcggtcaac ttctgccata atgggtcgat ccaaatggca gaccttttct ttcaatcttg
    66241 atttatcgat cgttctgatc tgctccaaca ggataacgga gtctctttct attcctgttc
    66301 cagtagcttt gatcccaatg tgagtcggaa gcttaggttt agccattttt gccgttatgg
    66361 ccgcaacaat gatcgtcggg ctaaaatggt tacctagatt attttgaata accaaaactg
    66421 ggcgtgttcc gccttgctcc gatcccacga ctggagaaag atcagcaaaa taaatatctc
    66481 ctctttttac cataggatcc tccttattct tgatattctc ttggtatccg ctgtcctaat
    66541 ccgcaagcca cttcatagtg gatcgtacct aattgatctg ctatatcctg catagtgatt
    66601 tctttgtctg catttttgcc gataagcgtc acttttgttc cgacaggaaa ttcttgaggc
    66661 aatcggatca tcagttgatc catacataca cgcccaatcg tttcacagta gttcccttct
    66721 accaatagag aaaaaccctg catctttcgt ggccagccat ctgcataacc gatcggtatc
    66781 gttccgatcc actctgcttc aggagtgata tatgtttctc catagccgat tccttcacct
    66841 gctgggagtt ttttgacttg aatcaattca gaaaccagct caagtgctgg ctgcaatgga
    66901 taaacttccg gtagtgcgtg cccagaggga ttaagtccgt acattgctac tccataacga
    66961 atcatattgc cgattgtttc atcatgccat aaagccgttg cactattgct gacatgtaca
    67021 tatcgaggaa gttcgggaag ttttttcagc acttctataa agcgttcttt ttgcagattc
    67081 caatatgtat catctgcctg atctgctgta gaaaaatggg taaataaacc ttcccattct
    67141 aatacacgac tttctttgat gaatgcagct gcctctttta cttcttcagg tgtacaaaat
    67201 ccgatacgac ccatcccagt gtctgctttt agatgaatcg aaagaggcgt ctctagctta
    67261 cttttctcta aaacagcagc tgctgcttgc aaccattccc gtgtacctgc agttactgat
    67321 aaatcatgag caacagctac agtcagtgcg tcgtaaggaa ccatattcaa gataaggatg
    67381 ggctgagtaa atccagcttc tctcaactcg actccttcat ctaaatttga aacacaaaag
    67441 cccgaagctc cacctgcaac tgctgcctca gctgtttcaa tagctccatg accgtatccg
    67501 ttcgctttta ctactgcaaa caattctttt ccttgcggaa gtcttgcaac ttcatttgct
    67561 acattttcag ttatagcttt tttatgtatc actgctttcg ttgggcggtg ccaagcgaca
    67621 accatcgttt ttccttcttt ctagtcttcc aaaataattt gtgcaaaagc tgtttcattt
    67681 gtatgagtaa tactgaccca tacattgcct tcatgaggtg aacgcgtcac aacaggctga
    67741 ccattttcat tttttaaaat ctcgacatct tgaaaggtaa cttttccgat cccagttccc
    67801 caagctttag agaaagcctc tttgcaagcg taacgcccgc ctaaaaattc tacttggcga
    67861 tgaaaaggca aggactggaa tagtttcatt tcatcagagg tcaagattcg tgcaatgaat
    67921 tttggttttt cttcgatcag ccgagtgatc ctaggaagtt cgactgcatc gatcccaatt
    67981 ccttttatca tatgcttttc tcttttcaat aaatttagaa cactctcatt ctaccaaatt
    68041 ataaccattt tgccacaaat aagtccacaa ataacaaaaa tgaaagtttc tcttaaaaaa
    68101 gagaaacttt cattttatgt ttaatcgtta tttgaacgga ttacaaatcc gcgtttttta
    68161 tcattgtgac gtttaccgcc atctttttgg ctgttattat ttttgctata accgcctttt
    68221 gatttgtttc cacgataatt accgccgtca cgacgattac ggttgtttcc gccaccacgt
    68281 ttgccatttt tattgtatcc ttttttctgc attggcaatg ggcgttctgg tgtgatcttc
    68341 actggcactg catcagctgg atcttttgct gtcgttttta gcaaaagtgc aactaaatct
    68401 tgtgcagaat attcttctaa taatttatct gctgtttgca ggtatttatc tagaccattt
    68461 tctgcaagtt ttgtttcgat ttgttcaact gcagcaccta attgaccttt gaatgcttct
    68521 ttttcggttg gtggacgtaa agtcgtcatc cgttttttcg tcaagttttc aatcacatgc
    68581 aagtagtcca tttcatttgg tgtaacgaaa gtcacagaca taccgccttt tccggcacgg
    68641 cctgtacgtc cgatacggtg aacgtagctt tctggatctt gagggatatc atagttgtac
    68701 acatgagtga cacctgaaat atctaatcca cgtgcagcaa cgtctgtcgc tacaaggata
    68761 tctaaatgac cgctcttaaa tgaacgcaat acgctcattc gtttttgttg agacaaatcg
    68821 ccatggattc cttctgcacg gtaaccgcgt gcttctaatc cgcgtgccaa ttcgtctaca
    68881 cggcgttttg tacgtccgaa gacgattgtt aattcaggtg tttgaacatc aaacaagcga
    68941 gtcatgatat cgaatttttc gtattctttt gcgcggacat agtattgatc gatcaagtca
    69001 gctgtcattt ctttcgcttt gatcttcaca tgatgtggat ttttcatgaa tttaacgcca
    69061 atattcttga tagctggcgg catcgttgct gagaaaagta atgtttgtct ttgatcagga
    69121 acttgtgaaa tgattttttc gatatcttcc aagaagccca tattcaacat ttcatctgct
    69181 tcatccaaaa ctaatgtttg aacggttcca agtttcaatg tgtggcggtt aatgtggtca
    69241 agcatacgtc ctggtgtacc cacaacgata tgtggacgat ctttcaagcc acggatctga
    69301 cggccaatat ctgctccgcc gtaaacggct tgtacacgta cttttttgtc gcgccctaaa
    69361 cgatataatt cttcttgtgt ttggatagct aattcacgtg ttggtgcgat aactaaacct
    69421 tgtaattcgt gacgatctgg atcgattttt tctaacattg gtaatccaaa tgcagctgtt
    69481 ttccctgtac ctgtctgtgc ttgtccaata acgtcttttc cagctaatgc tagtggaatc
    69541 gtttctgctt ggataggcgt tgcttcttca aaaccagcgc gttcgactga tttcaacaat
    69601 tctggtgata attctagttc tttaaatttc aaatgaatcc tcctatatgg ttgttaactg
    69661 aggatgtagc ttttttgcta ccccgtcgat ttgttcggtc aaagtgtatt gtttcttttt
    69721 gacagtggcc acgcacgttt ttatccattc ttagcccctg tggcaccacc gaaaaatcac
    69781 tttgcgaagt ttaggcatta gaaaagccaa acgggtcgcg ttcagcttta tcattattat
    69841 gacatctggc ttcaacaagt tgtttctatg gcaaaaccag tccaatttct cttcatcttt
    69901 cggcaatagg ttattctaac atagtttttc gttttctgca agtttacaga tttttatttc
    69961 atttgctgaa gttgttctac cacttcaaat aagcccatgc tgttgctgcc ttttagcata
    70021 atactatctg ttgcttcaag attttgtttg atagcatcaa tcaatttatt cttttctttt
    70081 tcaaaataga acacaggtag ttgggggtat ttttggttca attcttcttt tagtgcatgc
    70141 atttgttccc cataaaggaa aatcatggcg aatttctcag gatcgatatg ttcactcatt
    70201 tgcgcatgca tatttaaaga ctctttccct aattccagca tatctgctaa cacagcgatt
    70261 ctgcgtcctt ttgttggcag atttttaaaa gtatctaata ccagtcccat ggctgttgga
    70321 tttgcattgt agacatcgct caaaatagct gcaccatttc ctgccgttag ccattcggtt
    70381 ctgttttgag tgatagaaac atgggcgagc ccttggcgta tctcaggtat ggacagtcca
    70441 aagaagcgcc cgatcgtata cgcaatcaaa gcattcgtta cattataact tccaggcagc
    70501 gggatcgtaa atgtttctcc ttctactaca aaatcggttt gtgcttttcc ttttgctgtc
    70561 acttccgcat gaacatcgcc atttccaata ccgaatgttg tgactgtttg tactaatgtt
    70621 gccgttaaag gtgtcagcaa cggttcatct gcgggcacaa cgagtaatcc gtctgataca
    70681 agtccgtcag tgatttccat tttggcttta gcgattcctt ctcgcgaccc aagattttct
    70741 atgtgtgctt caccgatcat tgtgattgct gcagcttttg gttgagccat aagtgaaagt
    70801 tccgtgattt cattggcgtg atccatgccc atttccaaaa cgatcttttc tgtatcttgg
    70861 ggcatgtgca aaatcgtgta aggaagacca atattattgt tgtaatttcc ttgcgtctta
    70921 tatgttttgt atttttcaga caagacagac gcggttagat ctttcgtggt ggttttacca
    70981 ttgcttcctg taattgcaac gacttctgcg cctacttttt ccaagtaata ggttgccaat
    71041 tgctgcatgg ctttcaaagg atctgctaca ggaataactg gaaaattttt tggagcttgt
    71101 tcaacaggca agctccaaaa tgaagcagat gctccgtttg tgatcgcttg ctggataaat
    71161 tcatgtccat ctcgcgtacc agcaagagga acgaataaac ttccttcggt tatcttccgg
    71221 ctgtcaaatt cgacattggt aatctttgtc agtgcagagc attcgctgcc cacggcttgt
    71281 gcaatttctt gtattgttag attcattgag atctctccta aaaaaggttg tgaaaaaagc
    71341 gtttggccta aacggcgggt tcgcatgact ctcacttcca agtaaatttc tctattcgta
    71401 tttagaagct agaatgaacg cggttacgca atcactccag cctcttcttt atttctcttt
    71461 attcattttt ttcaaaaaaa gattgacgct gatggtatcg attcattcct aactggatca
    71521 gttcttcaat caaatcaccg tatttcaagc ccatattttc ccataagagt gggtacatac
    71581 tgaactccgt aaatcctggc atagagttta attcattcag gaataattca tttttatttg
    71641 tcaaaaagaa atcgcaccgg ctcaatccgc ttccacctaa catcgtgtaa gctaacttcg
    71701 cgtactcttg cgctttttga taaacttctt ctggcacttc ggctggaatc tgcatttcga
    71761 ttttattatt gatatatttt gcttcataat catagaatgc tacgtctttt acgacttcac
    71821 caggcaaagt cgtccgaacg tcttcatttc ctaatacagc aacttcgatt tcgcgcgctt
    71881 caattccttg ttcaacgatt gctcgagaat catactgata ggctgttgct aaagcatttt
    71941 gcagctcttc tcggttttct gcttttgtaa tgccgacact agaacccata ttcgccggtt
    72001 tgacaaacat cggataaagc aaagaacctt cacattgatc aaatactttt ttaggatttt
    72061 ctttccattg attcttaagt actggtacat aaggaacttg cggcacacca gcagcttgta
    72121 aaatatactt ggtcatgatt ttatccatgg cacatgcact ggtcaatacg cctgcgccga
    72181 cataaggcat attcaatgtc tctaagaagc cttggatcgt tccatcttcc ccgtttggcc
    72241 catgtaaaac tggaaaaacg atggctcctt cttctttgat ttcgcccgga ttgatcactt
    72301 ttcctgtaaa gccttcctct gtctgtccac ttgggtccca tgaaagatgc aagacatctt
    72361 tgctggcagg tttttctgtt agtaatggac ctttgaccca ttgtccttct tttgtaataa
    72421 atacgagttg aacttggtaa taattataat aaatggcatt taaaactgaa aatgcggaaa
    72481 gaatggacac ttcatgctct gcgctgcgtc cgccatatag taaagtaatc ttcaaaagat
    72541 tgtcctcctg tattctaaat cgttcagtga tttactctca tattttagca caaaacaaaa
    72601 ataaacgaat aacttattta taaaaaaacg agccagcttc taaaagaacg agcgtcattt
    72661 attttttgtc agctagatta tttgtcccgt tttttataaa aagttatgat aaagaaaagg
    72721 agggatgatg atggcgcaaa caaatccacg cagccgcttg gcagaaatca ttcgagtgtt
    72781 catcaattac aatgtagtgc caaactttgt acagcaaaaa aatcccgaac aggtaaaaaa
    72841 agcctttgaa gaactaggcc ccaccttcat caagatagga cagatgcttt ctgttcgaga
    72901 agatttgctt tcttctgcct tcactcaaac attcaaaaca ttacaagata gtgtacctag
    72961 cgacacattt tcaacggtca aaaagacgat cgaaacagaa ctttctcttt cattatcaga
    73021 tatattcgat gatttttcaa aatcgccttt tgcctctgct tctatggggc aggctcatcg
    73081 tgccaagctg aagaacggtg acgcggtagt cgttaagatc caacacccta acatcgccga
    73141 agagatccga cttgatcttc aactatttga acgagcgatc cctttgatca aatacattcc
    73201 tgaaactagc gtagttgatc taaaaggggt actccaagaa gtaaaacgtt ccttgatcaa
    73261 tgaaatggac ttcttaaaag aaagtcaaaa tggcgaacaa ttctatcaaa agaacaatgg
    73321 ctggaaagag atccgcagcc ccaagattta tgacgctttt tgttcgaaaa aagtgatcgt
    73381 gatggaagaa atgtctggta aaaacttgaa tcatttaatg aacgccgaaa acaaaactga
    73441 aacttttatc actggaattc aaaacaagca gctgaaacaa gaagttgcaa agcttttagt
    73501 tgagaacttt atgaaacagg tgtttgatga tggttttttc catgctgatc ctcatccagg
    73561 gaatctgcta ttccatgtac taacaaaaga agaacagaca caagctagcc gaaaaactga
    73621 aacagtccat gaaaaagagt tcggttcctt tgcttttcga gcctccactt ctgcagaaga
    73681 ccctgttgcg ccatatacca tcaactacat cgattttggt atgatggggc atctttctgc
    73741 aggacttagg caaaaactca cacaagccgt tttagcgctg tatacgaaag atgcttatcg
    73801 aatcgaaaaa gcggtccttc gactttgtca gcaagaaggc tcgtttgatg aaagtcggtt
    73861 ccaccaagaa ctgacaagtt ttcttgaaca gtattatgat tctcctattg atgaaatcaa
    73921 cttgcaagaa gtattcacac atgtcgtgac gatttgtcat caaaataatc tgcagttcga
    73981 tcgtgatatc acgcttttgc tgaaagcatt tgggacatta gaaggggtga tccgcgtcct
    74041 cgatcctgaa gtttccctca tggaagtcgc ttcgcctttt gctcaacatt attttctcac
    74101 tcatctggat gtagaagata cattgaaaca aagcggatta gacttattgg aagggatgaa
    74161 ggctgcgcct aagatccctc aacagctcca ccatctatta gaaatgtgga cttcgggaca
    74221 aggaaaagtc aatttggaat tgaaaaagca agacaaatta ttgagcagga tcgaaagtat
    74281 gatcaatcgg cttgttttcg gtatgatttt agctgcattg atcgtagggt cctccctgct
    74341 tgtccaagca gcacctgtag aaaacgcaga ggtcgtttcc ttattaggga tatttacgta
    74401 tgctattgcc gcattcgtca ttattttttt ggcaatcgat gcattgatcc aaatgtataa
    74461 gaagaggaaa aagtagaaaa aggttgtgac agaaagaatc agcttcaaga aataagaagg
    74521 ggttttacat aaattgtttc tcaaattttt gtgaaaattc ggcttatttt tcgaagaggc
    74581 tacttctgaa acaccgagaa taaaaaatgt gaaaaagctg ttctgcaccc agtaataaga
    74641 acaatcaaaa agggaacatg gaacacattt atcctgtttc cctgttccct ttttgatgta
    74701 ttcgagcata aataaaatac cttcgttacc tactatcttc tgtacttggt aaaccttagt
    74761 caattgcctt ttttaacgct tgacgaaaag acatatgatc tttctccgta aagggttttg
    74821 gaccctttgt ccttatgcca gcttttctta actttccact atgataacgt tgttctagaa
    74881 gcgtgccgat attcgcttcg tcaaattctt ttccagagtg atggaacact cgcacaccct
    74941 tcggcaatac aagtgaagct aaaccataat cttgcgtgat caaaaagtca ttcttcttaa
    75001 ccataccgac gattttataa tccgcactat cagctccgcg gtctacgtag acgatttgga
    75061 catgattagc gtattctttg gtcgtataat ggtccacact ggtcacgata accacgtcaa
    75121 gaccatagga ttctcctaat tttatgacat cttcttttac tggagaacca tctccatcaa
    75181 tgaatagcct cattcttttt cgccttcttc cataaatgat aaacagttac taatccgctc
    75241 agtaaaagac caacattgat ccattttgcc aagataagca gttgtacact ttctatcggc
    75301 actgattcat ggttcagcgt gatcaataat agatggattg gctctcccat ttcttttgct
    75361 aaaacaaaca aaagcgtcat caataaaaac tggtggatca cactcccttt attttgagta
    75421 gtatacttga caacgatccg gcgatccatt ggaatagctg tatcagtttg ataatattcc
    75481 ttatttagcc agctgcctgt tactacgtgc cagaaactaa gcagaaagac acctatcgca
    75541 aaaccatttg gaatcaagag gaccaataaa gaagaactca gtccaagcaa ttgcatagcc
    75601 aatggagacc atgttaaacg tttcaaaatc catggtccga ctatttttgc tgcaatcaag
    75661 cctgctgcat aaggcaaata cataaaaatc gaaagttttt ctaatccgta cagtatgcca
    75721 acataaaaac tgctaaataa aaaaaggaaa ttccctagca ttccatttag aaaagtaaac
    75781 agatttagcc agcttggcaa cttccattcc ttcttctggt gtcctaaaac aaacacgaac
    75841 aagatgaaca gcagcaagaa accataaata gcaagatcaa aaagcctttc atcgaataac
    75901 aatcgggcat ttctcaaaag aaacaataag ccaaagaata tcaaaaataa aacaagctct
    75961 cgcgtagcta ccagatttct tttgatgtct ttgaaatcca gttcgtaacg cggataatga
    76021 ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa tagtaaagct tgctgcaatc
    76081 tgagtcggtg caggcaactc aatcgcttga tacaggagaa atagccagat agctgcaaat
    76141 aagtactttt tcccatccat attgataaaa ccttgcgtct tttcgtgaaa attgaccgtg
    76201 gtattagcag gtacaagcca tgctgcactc agccctagca aaacacttga aaaataaaat
    76261 aaaggaaaat aaaatacgcc taatattcca agcaatgaac cagcaccacc aatcagcaga
    76321 gcgatcatca gcaacgtgta actatctaga ccaaatcgta gactgttgac taaaaatagt
    76381 ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa tcgtcagcca attatgcgag
    76441 tctgttagtc ccaaaaataa aagataagga ataaaaataa ccgtgttact taagataaag
    76501 ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt tttcgaacat gacaatggct
    76561 ccttaaacag tcttataact aatctgctct tcgattttag cattaaaggg gaaataaacc
    76621 aattttacag aatatattct tgtaaaaaaa aatacagaaa gaagcataag cttctttctg
    76681 catttttacg gtacttgatt actcaagcag acaagccctt ctccacaaaa gtcttgcccg
    76741 acacccctgc atccggcagg tgataaagat agaaatcaat tatcctgtgc ctccaaaatc
    76801 aattaggagc tctgtaaacc agagtccttc tcgacccgtc gcataaattt actttaacac
    76861 agttcctatg tttatgcgag agcttttttc attttataat cgaccatttg aaatccttga
    76921 ccgatatctg aaataagttc ttctgtaatc tcaaatcctt catgcttata aacctcgatt
    76981 gcttgatgat tcccctgatt cactcgcaaa aagatctctc tttcctttgt ttgcgcagca
    77041 agttcttcat accagcgaaa aactgaagaa gtcaatcctt gaccacgtac tttatttagc
    77101 aaatataatt tacttaaata aatttttcca tttatctctt cataagcagt gtaaccgaca
    77161 gtcttttctt cgctttttaa taaaaagtag gacacacctt ctgtcagttc tttttgaatc
    77221 tgtttttttg actgataagt tttcagcatg taatccactt gtttttgacc aatgatcggc
    77281 acaaaaactt ccggccatac ttcttgtatg actgccatca gctcatccag ttcattttct
    77341 gttactttta ttttttccat acacccacgt ccttttttac attataaatg accttgcgct
    77401 tgatggaata aaaagagcat gatcatgcta tacacaccca ccgaccacgg tttcaagaaa
    77461 atcacaatct gcataaattc cctaaaagat aatttcgtca aaccagctac tagacagatc
    77521 aagtcgtctg gcgcaaaggg aagcagcaag gtaacaacta gtactttttt aatatctttc
    77581 gtttttcttg tcagcaattc atcaaatttc tgatactttt ttggcgataa aataagctgg
    77641 acaaaggcta cgccatagta acggacaagc aaaaatccga ttgcttcccc aatcactagg
    77701 ccaatacaac tatataaaag cccaaatcca ttgccaaaca tcagcatgcc cgctactgag
    77761 gagatccctc ccggcaaaat aggaatgatc acttggatca cttgcaacag aacaaatatc
    77821 atcactgcat acttgccaaa ttgctggata aacgcttgca aagacgtgct cgatcgaaaa
    77881 atcccatgat gataagcata aagaatcaac acaaagaaaa aaaacagacc aataatgggc
    77941 atgatctgaa gacatttttg taaaactgat ccttttttag acatctatat tccccacatc
    78001 ttttttcatt tattaaaact gcctccttat gaactgaacc ccaaaagttg aactatttaa
    78061 tggactgttt ccgatattct actggagata gtccatttaa ctttagtttt atccttttgt
    78121 tattatacca cctaatatac tcatttaatt tactttgaaa tatctcaatt gatcggaatt
    78181 tttctcgata aaaaaactct gatttaagca caccaaaaaa attttctatt acagaattat
    78241 ctaagcaatt tccttttcta gacatgcttt gaataatgtt attctctttt aatttttttt
    78301 gatattgtgg catctgatac tgccatcctt gatctgaatg tagaatcagt gaaccctcag
    78361 ttcccttttt tttaattgct tgttgaagca tttcttcaat cagtttatat gttggactag
    78421 ttgatatact ataactaatt atttctccgt taaataaatc aagtatagga gatagataga
    78481 tttttcttcc ttttatcttg aattcagtga cgtctgtcac ccatttttta ttgggtgtat
    78541 ctactgaaaa atttcgtttt aacacattct tggcaatttt tcctactgtt cctttataag
    78601 atttatatcg ctttattcgg atttgacagg taagtcccat ttgggacatt aattttctaa
    78661 ctgttttatg attgattgta tatcctttca tctttaacgc taaagtgact ctacgataac
    78721 cataagagtt tcttgattct tttacaatcg ctgtaatttc ttgctttatc ttgctatatt
    78781 tatctggctt atctaatttt tttacccagt aataataagt tgacttcgct aattgtgcaa
    78841 ttgaaagtaa tagattcaat ttaaattctt ttttgagctg aaggattgtt ttaaccttga
    78901 tttcttcttg ctcaaatctt gttcttgaat caaggtttct aactttttta aatatgcgtt
    78961 ctctgctctt aaacgaataa cttcttcttc aagagactca tctttaagtt ttttaggaat
    79021 gtttagcttg gaattcatac taatttttct gccccttttt tggctctcaa gtgaagaagc
    79081 accgccttct tcatattgct ctatccattt acttagagtt ctatttgaac cgatattaaa
    79141 ttttttagca gtttcttgga tagaaagacc atttgtttcc atatattcta taacatcaag
    79201 tttaaatttt gtagtgtagc ttttgccacc tccaaccaag ccttcccaac catgatagtt
    79261 ataaatcctt acccaatgtc taaccagtgt acgatttatt tgatatttat gtgcaagata
    79321 tttgtagccg ccttcgttat ttaaatagtc tgaaactact tttttcttaa aaacaaatgt
    79381 atatttccgc aaaaaaagca ccccttttaa ttagatttct agtc
//
LOCUS       DAZKOB010000004        76064 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000004 DAZKOB010000000
VERSION     DAZKOB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.004"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..699
                     /locus_tag="ACG8M6_000256"
     CDS             67..699
                     /locus_tag="ACG8M6_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455956.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(833..958)
                     /locus_tag="ACG8M6_000257"
     CDS             complement(833..958)
                     /locus_tag="ACG8M6_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0455957.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1092..1985)
                     /locus_tag="ACG8M6_000258"
     CDS             complement(1092..1985)
                     /locus_tag="ACG8M6_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0455958.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2167..3093)
                     /locus_tag="ACG8M6_000259"
     CDS             complement(2167..3093)
                     /locus_tag="ACG8M6_000259"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0455959.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3178..3939)
                     /gene="pflA"
                     /locus_tag="ACG8M6_000260"
     CDS             complement(3178..3939)
                     /gene="pflA"
                     /locus_tag="ACG8M6_000260"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0455960.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4183..6414)
                     /gene="pflB"
                     /locus_tag="ACG8M6_000261"
     CDS             complement(4183..6414)
                     /gene="pflB"
                     /locus_tag="ACG8M6_000261"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0455961.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6691..9141)
                     /gene="parC"
                     /locus_tag="ACG8M6_000262"
     CDS             complement(6691..9141)
                     /gene="parC"
                     /locus_tag="ACG8M6_000262"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0455962.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9153..11198)
                     /gene="parE"
                     /locus_tag="ACG8M6_000263"
     CDS             complement(9153..11198)
                     /gene="parE"
                     /locus_tag="ACG8M6_000263"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0455963.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11364..11798)
                     /locus_tag="ACG8M6_000264"
     CDS             complement(11364..11798)
                     /locus_tag="ACG8M6_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0455964.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11925..12563
                     /gene="plsY"
                     /locus_tag="ACG8M6_000265"
     CDS             11925..12563
                     /gene="plsY"
                     /locus_tag="ACG8M6_000265"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0455965.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12695..13570)
                     /locus_tag="ACG8M6_000266"
     CDS             complement(12695..13570)
                     /locus_tag="ACG8M6_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0455966.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13652..14443)
                     /gene="codY"
                     /locus_tag="ACG8M6_000267"
     CDS             complement(13652..14443)
                     /gene="codY"
                     /locus_tag="ACG8M6_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0455967.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14461..15861)
                     /gene="hslU"
                     /locus_tag="ACG8M6_000268"
     CDS             complement(14461..15861)
                     /gene="hslU"
                     /locus_tag="ACG8M6_000268"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0455968.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15875..16423)
                     /gene="hslV"
                     /locus_tag="ACG8M6_000269"
     CDS             complement(15875..16423)
                     /gene="hslV"
                     /locus_tag="ACG8M6_000269"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0455969.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16460..17365)
                     /gene="xerC"
                     /locus_tag="ACG8M6_000270"
     CDS             complement(16460..17365)
                     /gene="xerC"
                     /locus_tag="ACG8M6_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0455970.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17563..18939)
                     /gene="trmFO"
                     /locus_tag="ACG8M6_000271"
     CDS             complement(17563..18939)
                     /gene="trmFO"
                     /locus_tag="ACG8M6_000271"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0455971.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18908..20986)
                     /gene="topA"
                     /locus_tag="ACG8M6_000272"
     CDS             complement(18908..20986)
                     /gene="topA"
                     /locus_tag="ACG8M6_000272"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0455972.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21108..21965)
                     /gene="dprA"
                     /locus_tag="ACG8M6_000273"
     CDS             complement(21108..21965)
                     /gene="dprA"
                     /locus_tag="ACG8M6_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0455973.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(22021..22788)
                     /locus_tag="ACG8M6_000274"
     CDS             complement(22021..22788)
                     /locus_tag="ACG8M6_000274"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0455974.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22781..23641)
                     /gene="ylqF"
                     /locus_tag="ACG8M6_000275"
     CDS             complement(22781..23641)
                     /gene="ylqF"
                     /locus_tag="ACG8M6_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0455975.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23901..24299
                     /locus_tag="ACG8M6_000276"
     CDS             23901..24299
                     /locus_tag="ACG8M6_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0455976.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24536..25078
                     /locus_tag="ACG8M6_000277"
     CDS             24536..25078
                     /locus_tag="ACG8M6_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0455977.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25161..25712)
                     /gene="lepB"
                     /locus_tag="ACG8M6_000278"
     CDS             complement(25161..25712)
                     /gene="lepB"
                     /locus_tag="ACG8M6_000278"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0455978.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25864..26091)
                     /locus_tag="ACG8M6_000279"
     CDS             complement(25864..26091)
                     /locus_tag="ACG8M6_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0455979.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26088..26606)
                     /gene="msrA"
                     /locus_tag="ACG8M6_000280"
     CDS             complement(26088..26606)
                     /gene="msrA"
                     /locus_tag="ACG8M6_000280"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0455980.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26626..27339)
                     /locus_tag="ACG8M6_000281"
     CDS             complement(26626..27339)
                     /locus_tag="ACG8M6_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0455981.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27342..28238)
                     /locus_tag="ACG8M6_000282"
     CDS             complement(27342..28238)
                     /locus_tag="ACG8M6_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0455982.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28396..29238)
                     /locus_tag="ACG8M6_000283"
     CDS             complement(28396..29238)
                     /locus_tag="ACG8M6_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0455983.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29370..30023
                     /locus_tag="ACG8M6_000284"
     CDS             29370..30023
                     /locus_tag="ACG8M6_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0455984.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30086..30613)
                     /locus_tag="ACG8M6_000285"
     CDS             complement(30086..30613)
                     /locus_tag="ACG8M6_000285"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0455985.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30633..31580)
                     /locus_tag="ACG8M6_000286"
     CDS             complement(30633..31580)
                     /locus_tag="ACG8M6_000286"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0455986.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31592..33475)
                     /locus_tag="ACG8M6_000287"
     CDS             complement(31592..33475)
                     /locus_tag="ACG8M6_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0455987.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33638..35716)
                     /locus_tag="ACG8M6_000288"
     CDS             complement(33638..35716)
                     /locus_tag="ACG8M6_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0455988.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35729..36004)
                     /locus_tag="ACG8M6_000289"
     CDS             complement(35729..36004)
                     /locus_tag="ACG8M6_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455989.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36366..36821)
                     /locus_tag="ACG8M6_000290"
     CDS             complement(36366..36821)
                     /locus_tag="ACG8M6_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455990.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36975..38183)
                     /locus_tag="ACG8M6_000291"
     CDS             complement(36975..38183)
                     /locus_tag="ACG8M6_000291"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0455991.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38295..38627)
                     /locus_tag="ACG8M6_000292"
     CDS             complement(38295..38627)
                     /locus_tag="ACG8M6_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0455992.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38775..39650
                     /locus_tag="ACG8M6_000293"
     CDS             38775..39650
                     /locus_tag="ACG8M6_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0455993.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39732..40313)
                     /locus_tag="ACG8M6_000294"
     CDS             complement(39732..40313)
                     /locus_tag="ACG8M6_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0455994.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40319..41578)
                     /locus_tag="ACG8M6_000295"
     CDS             complement(40319..41578)
                     /locus_tag="ACG8M6_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0455995.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41580..42626)
                     /locus_tag="ACG8M6_000296"
     CDS             complement(41580..42626)
                     /locus_tag="ACG8M6_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0455996.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42861..43136)
                     /locus_tag="ACG8M6_000297"
     CDS             complement(42861..43136)
                     /locus_tag="ACG8M6_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0455997.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43379..44689)
                     /gene="der"
                     /locus_tag="ACG8M6_000298"
     CDS             complement(43379..44689)
                     /gene="der"
                     /locus_tag="ACG8M6_000298"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0455998.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44871..46100)
                     /gene="rpsA"
                     /locus_tag="ACG8M6_000299"
     CDS             complement(44871..46100)
                     /gene="rpsA"
                     /locus_tag="ACG8M6_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0455999.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46235..46915)
                     /gene="cmk"
                     /locus_tag="ACG8M6_000300"
     CDS             complement(46235..46915)
                     /gene="cmk"
                     /locus_tag="ACG8M6_000300"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0456000.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46964..47599)
                     /locus_tag="ACG8M6_000301"
     CDS             complement(46964..47599)
                     /locus_tag="ACG8M6_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0456001.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47665..49068)
                     /locus_tag="ACG8M6_000302"
     CDS             complement(47665..49068)
                     /locus_tag="ACG8M6_000302"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0456002.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(49055..50086)
                     /locus_tag="ACG8M6_000303"
     CDS             complement(49055..50086)
                     /locus_tag="ACG8M6_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0456003.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50117..50338
                     /locus_tag="ACG8M6_000304"
     CDS             50117..50338
                     /locus_tag="ACG8M6_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0456004.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50503..51240)
                     /locus_tag="ACG8M6_000305"
     CDS             complement(50503..51240)
                     /locus_tag="ACG8M6_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0456005.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51393..52733)
                     /locus_tag="ACG8M6_000306"
     CDS             complement(51393..52733)
                     /locus_tag="ACG8M6_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0456006.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52970..54493)
                     /locus_tag="ACG8M6_000307"
     CDS             complement(52970..54493)
                     /locus_tag="ACG8M6_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0456007.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54871..55482)
                     /locus_tag="ACG8M6_000308"
     CDS             complement(54871..55482)
                     /locus_tag="ACG8M6_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0456008.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55605..55730)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55812..56528)
                     /locus_tag="ACG8M6_000309"
     CDS             complement(55812..56528)
                     /locus_tag="ACG8M6_000309"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0456009.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56534..57103)
                     /gene="scpB"
                     /locus_tag="ACG8M6_000310"
     CDS             complement(56534..57103)
                     /gene="scpB"
                     /locus_tag="ACG8M6_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0456010.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57087..57857)
                     /locus_tag="ACG8M6_000311"
     CDS             complement(57087..57857)
                     /locus_tag="ACG8M6_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0456011.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57854..58225)
                     /locus_tag="ACG8M6_000312"
     CDS             complement(57854..58225)
                     /locus_tag="ACG8M6_000312"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0456012.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58244..59131)
                     /gene="xerD"
                     /locus_tag="ACG8M6_000313"
     CDS             complement(58244..59131)
                     /gene="xerD"
                     /locus_tag="ACG8M6_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0456013.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59227..59700)
                     /locus_tag="ACG8M6_000314"
     CDS             complement(59227..59700)
                     /locus_tag="ACG8M6_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0456014.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59805..60668)
                     /locus_tag="ACG8M6_000315"
     CDS             complement(59805..60668)
                     /locus_tag="ACG8M6_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0456015.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60864..62963)
                     /locus_tag="ACG8M6_000316"
     CDS             complement(60864..62963)
                     /locus_tag="ACG8M6_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0456016.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(63183..64292)
                     /gene="rpoD"
                     /locus_tag="ACG8M6_000317"
     CDS             complement(63183..64292)
                     /gene="rpoD"
                     /locus_tag="ACG8M6_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0456017.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(64316..66157)
                     /gene="dnaG"
                     /locus_tag="ACG8M6_000318"
     CDS             complement(64316..66157)
                     /gene="dnaG"
                     /locus_tag="ACG8M6_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0456018.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(66339..68654)
                     /locus_tag="ACG8M6_000319"
     CDS             complement(66339..68654)
                     /locus_tag="ACG8M6_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0456019.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68839..69699)
                     /locus_tag="ACG8M6_000320"
     CDS             complement(68839..69699)
                     /locus_tag="ACG8M6_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0456020.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69758..70390)
                     /locus_tag="ACG8M6_000321"
                     /pseudo
     CDS             complement(69758..70390)
                     /locus_tag="ACG8M6_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            70557..71318
                     /locus_tag="ACG8M6_000322"
     CDS             70557..71318
                     /locus_tag="ACG8M6_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0456021.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            71344..73935
                     /gene="mprF"
                     /locus_tag="ACG8M6_000323"
     CDS             71344..73935
                     /gene="mprF"
                     /locus_tag="ACG8M6_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0456022.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYIKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(74123..75310)
                     /gene="tuf"
                     /locus_tag="ACG8M6_000324"
     CDS             complement(74123..75310)
                     /gene="tuf"
                     /locus_tag="ACG8M6_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0456023.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            75678..76034
                     /locus_tag="ACG8M6_000325"
     CDS             75678..76034
                     /locus_tag="ACG8M6_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0456024.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        20768 a        16029 c        13052 g        26215 t
ORIGIN      
        1 catcgacata tactcatcaa tttgttatta tactttatat acttatcaat ttaatttgga
       61 gatattatgg aaattcaacg tagaaccact aattttttac atttaggctc tttttttgca
      121 actttgacga tcttattttt tctatgttat tcatacctta agacctcaca tgcgccttct
      181 agtgcattag gctggttgct tttggatcat tctgatatca cagaagccga agaaaatccg
      241 ttttttttga ttttgtcttc tctttgcggc ttactgtttt ctgtttattt cggtgcctat
      301 ttttttcatc gacatcctgg tacgttaaac gaagcttcaa gcgtattgat tgcatttaaa
      361 acgttttctt ttttagcaat tcttttttat tcgtttaatc acaatacgct tttcttcttt
      421 atcatgaatg gtttagtcgt gattttatcc atttttactt attttatttt ttcactcatt
      481 tttagccaat tgaaatgtcc gctcttcttt cgattagttc ctgtcacctg ttttatttac
      541 agcttgtttt ccttttttgt ccttgcttta atgccactga ccaacccttc gacgatccaa
      601 gtcgcaggaa atctgttgga tatgctcttc atcgtcagtc taggtgtatt tatgtggcaa
      661 agaaaaaaca aacaaaataa aaagcatttt gaagcctgac tattacgact tcataaatgc
      721 ttttttagtt ttaaacactt caatctaaaa gaataaccca agattggata aattattaat
      781 gactcgccat tcgtttctta gtgattctgg attatcaaat ggacttccta atctaatcag
      841 ctagttcttc ccataaaatg ccatacttga ctgaatcata atattgatta tttaaaaatc
      901 taacttttct tatcactact tcctttgtca ttccacattt ctcaccattt ttttgcattc
      961 ctttgtttcc agaccatgta gtgaatctaa taggtggtag ataggcaaaa tactcaaata
     1021 attcttttaa ccataaagga agagaaaggc tcctcctgca agagtcagta taatttttgc
     1081 ttactgctct cttaggtttt actcactaaa actgttccat attctttgat ataagaaaat
     1141 gcatgcggta taaatttctt gctcagctca gaaaagattt gtcgtttaag aaaggctatt
     1201 tcctccattg tcaatgaagg atccaatgtt ggtaattcaa tctcaaaatt gtttttttcg
     1261 acaataggta attctaatgt gaatgattct tcttccttta tagtattcgt cacaagtaga
     1321 gactgatgga aaatactagg ccggcgattt ttatatttat tcttacgtat cctgtcaacg
     1381 ataacttcta tttgatcaaa aaagtacgat tcatttacgt ctccaataag ttcggctaat
     1441 tctttgaatg ccttcgttac aggaagctga ttcttctgta caaaaaagta attagccaaa
     1501 gtaagcggga agtgttctat agaggaaagt gtgataaaac ggtacttctc atttcctgca
     1561 gttgtcctca gcaattctgt ctctcggctg tttcttactc cataaaaata cgcttctttc
     1621 cgttcatctt cgaaacaata cgcccaaata acttctccca tcgctaattt ttgttcatca
     1681 acagagagtc tcttcaattg ttcagctatg gtttcagcag ccgaagtggc ttgttgcaag
     1741 taatctttag gatcaaatat tggaaagttc aaatgatatc ttcgttcttc cctcttgatt
     1801 aatcctgcat cgatcaataa atcaagaaaa tgggaaaatt tattttgcga aaattctttt
     1861 ttcaattctc ttagaatcgt ttcttgttct cgatgttcat atagataatc ggtcaatgaa
     1921 gaaaaaagcg gatcatcggt cagtttttga taaaagtttt ccttattttt tgaataatag
     1981 ttcatatatg ttctcttttc tatccgtttt attttactta aaaggctttt acgtaaaaag
     2041 gaggaagaac aagagtcata attgactgtt attctcctcc atttcttcgg cctttagtat
     2101 gttctatgtt tctttttata tcaatcgctt atccattcat tttgaatgct tcatactacc
     2161 atttgattag ctaaatacag ccgtcaattg aggtacgact tgttttttac gtgaaacgac
     2221 acctttaagc aaagcacggt tgttggtcaa agttgtttgg aaagcttctt ctactttttc
     2281 ttttccttta ccagcaacaa gtaattctga atcactgtct aagatgtttg tgacaactaa
     2341 tacgaacaga tcgtagccat ttgctttcat ttcatcttcc attgctgctt ctagctctgc
     2401 ttgtcgatcc atcacttcat taaggtccac tgtattgact tgagcgatac gaacgttgga
     2461 actgcccatt gggaaacttt ttgcatccaa atcaagaaga accgcagctg atttatcact
     2521 tagatttgtt cctgctttca acatttcaag tccataagtg tttagatcga cttcagcgat
     2581 tgctgcaagt tcttttgcag ctgcttcatc ttcaggcgta catgttggtg atttgaacaa
     2641 caatgtatct gaaatgatag ctgagagcat gattcctgcg atttctttag gaattgttac
     2701 ttggttttct ttatatagtt ttaaaataat cgtacttgta catccaactg gttctgcacg
     2761 atagtacaat ggattagctg tttcaaagtt tgcaatacga tggtggtcta ctacagctaa
     2821 aatatttact tcgctgatat ctgaaatact ttgttggaat tcattatgat ccacaagcat
     2881 gacatctgca ttttctgctt tagctgactc tactactcgt ggtgcagata gctgaaagtg
     2941 gtctaatgcg tattgtgttt cttcattagg tgtacctaga gcaactgctt ctgtttcttt
     3001 acctaattca ttttgcaagt gtgaaaacgc aatggctgcc ccgattgcat ctgtatctgg
     3061 attttggtga ccaaaaacta aaatttttga catgtcattc gctcctttgt gatttctcct
     3121 atttaataat agcaaaaaaa cggaaagaag caatacttat cttctttccg atgcgaccta
     3181 tcgcgttaaa tattttgtat actcttctgt atgaagcaat cgtttagcat tttcgacacg
     3241 ttcttttgta ggtggttcga tcccttctaa tggatatttc aaaccaagtg tttcccactt
     3301 gtattttccc atcgtatgat aaggaagaat ctctactctg tctacatttg acaaggtttg
     3361 aataaagttg tctagtcgga tcaaatattc atcataatca cttcgctcag gaactagtac
     3421 atggcgaatc caaaccggtt tgttgatttt tgacaaatat tgagccattt ctaaaatatt
     3481 ttcattgtcc caatgcgtta gttttttatg ctcttcatta tcgatgtgtt tgatgtcaaa
     3541 taacagtaaa tctgtatact tcatcaattc ttgaaaacgt gaaaagaaag gttcttcata
     3601 cgtaaaaggt tttccgcatg tatccaatgt agtatgcatc ttccgctctt ttgctttttt
     3661 aaatagttcg atcaaaaaat cgatctgcaa tagaggttct ccaccactaa cagtaatacc
     3721 acctttatct ccccagtaag gtctgaagcg ttctgcttta tctaaaagtt catctgctgt
     3781 gtattctttc cctccgccaa tattccaagt atctggattg tgacaaaatt ggcaacgcat
     3841 gcggcatcct tgcataaaca cgacaaagcg cagtccaggt ccgtctactg aaccgaaagt
     3901 ttcgatagaa tgaacatagc cgattgtttt ttcttccatt taagttcaca tcttttcttt
     3961 gatttcattt taagtacaag ttagctgtcg aagcgaagct ttctgaacaa aatttttcgc
     4021 aattgccgct tcgttatgtg taaaatttac agggacggaa catacaagtc ctgcccctgt
     4081 aaatcgtttc tttcaaataa gcatactgat tattcgattt tgatgcgatg gctattcaac
     4141 gtcctttcga cgctaagtcg aaaaactttt ttcacgaact ttttacattc tgtcgtgtga
     4201 agttctcatc aagacatcta attgttgttc tcttgtcaag tcacggaatt taactgcata
     4261 accagatacc cggatcgtca agttcggata tttcattgga ttttccattg catcacgcaa
     4321 taaatcatta ctgaatacat tgacattcaa atgataagct ccttttgaga agtatccatc
     4381 taagacatta cgcaagtttt cgattcttgt atcttgttct tttccaagac ctgaaggatt
     4441 gattgtttgg gtatttgaaa ttccatccaa tgcacagccg taattcattt tagctgttga
     4501 gttcaggcta gctaataacc cactttgttc cgcattgtaa cttggatttg ctcctggcgc
     4561 aagtggtttt ccagcttcac ggccatcagg agtatttcct gtatttttac cgtaaacaac
     4621 atttgaagta atcgtcaata atgaagttgt tggttttgag ttgcggtaag ttttatgacg
     4681 tttgatttgt cccatgaagt attccaagat ccatgaaact aattcatctg cacggtcatc
     4741 atcatttccg tatttaggga agtcgccatc cacttcaaaa tcaactgcta ttcctttttc
     4801 attacggatt gcttttactt ttgcatattt gatcgctgac aagctgtctg ccgcatgaga
     4861 gatcccagca atacctgtag caaatgtgcg ttgcaagtca gaatccatca aagccagctg
     4921 agctgcttca taagaatatt tatcatgcat ataatgaatg atgttcaacg tattgacata
     4981 tagttctgct aaccaatcca tcatattcat gaagttatgt ttcacttctt caaagtctag
     5041 tatttctgat gtgattggac gatattctgg tccaacttgg gcgccagtca cttcatcgac
     5101 tccgccattg atggcatata atagtgtttt agccaagtta gcacgagcgc caaagaattg
     5161 catgtctttt ccgacaactg ttcctgatac acagcaagca atcgcacaat catcagaacc
     5221 ccattgttca cgaagctgat tatcattttc gaattgaatc gaagaacttt cgatagcgat
     5281 tctgctagca tatgttctga atccttctgg aaggtgttca gaatacaaaa cagtcaagtt
     5341 tggttctggt gaaggtccca tatttgttaa tgtatgaagc agacggaaat cattctttgt
     5401 aactaatgaa cgaccatcta atcccatacc tgcaatggat aaagtagccc agatagggaa
     5461 tcctgagaat aattggttat attcaggtgt acgcgcgaat ttgaccatac gtaatttcat
     5521 tactaagtgg tcgatcaatt cttgtgcttc tttttctgtg attactccat tatccaaatc
     5581 acgttggata tagatatcaa ggaatgctga gatacgtccg attgacatcg cagcaccatt
     5641 ttgtgatttg attgctgcta aatatccgaa gtatagccat tggacagctt cttttgcatt
     5701 tgcagctggt ttagaaatat catagccata tttcgcagcc atttctttca tttgcgctaa
     5761 tgcacgatat tgttcagtga tttcttcacg taaacgaatc acttcttcac tcattgtatg
     5821 ataaccagtc atatcatgat cttttttctt ttgttccatc aaatagtcga ttccatacaa
     5881 tgccacacga cgatagtcac cgatgatacg tccgcgaccg taagcgtctg gaagccctgt
     5941 aatgattttg tttttacgag cagcacgcat ttcagcagta taagcgtcaa atacaccttg
     6001 attatgtgtt ttgcgccagt cagtgaaaat atgtgtcaat tcatcgtttg gtgtatagcc
     6061 attgcttttt agcgcattgt tagccatatt gattccgccg aacggcatga atgcttgttt
     6121 caaaggtttg tctgtctgta gaccgacgat tttttccaaa tctttatcca aatagcctgc
     6181 atcgtgtgaa gtaatagttg atacgatttc tgtgtccata tcatagacac cgttacgctc
     6241 gaattggata tcatttaact tttgcaattc atcccataat ttttctgttg cttctgttgg
     6301 gccttccaaa aagctgtcat caccgcgata ttcagtgtag ttattttgaa taaagtctct
     6361 agtgtctacg ccttctttcc atttggtacc tttaaatcca ttccattgct ccattgtatg
     6421 gacctcctaa atttctgttt tatgtaacag tttttacgtg attagtataa cacagcaccc
     6481 tatctttgca agggttttct tgttttttat ttaacaattt ttatgtttta tttacatata
     6541 tttaaatcaa cgttgacata ccaacattta agcattgtga acactttcat ctttttttgc
     6601 aaacgtataa ggatttatta aaaatgaatg aaaaagagac gccacctgta ttaatacaga
     6661 tggcgtctct tcattaaaat actacaacag ttacgactcg atgaaaatct caggagcatc
     6721 gataacttcg atgacttctc cacctttttt ctcatccatc gcaaatgatc cattcgatgt
     6781 tcgatcacct atagggaaat cagttgggtc aatcgttaac tgctgccctt tttgattcat
     6841 caaggttatt tcttttgatc ttccttccga catatagata atacggtgcg gatttttttt
     6901 cagctcacgc atgaccgtca ctccccgctt cgcacgtgaa gtttggctga tttcttgagc
     6961 aagcatgcgt ttaacagctc ctcgctgagt aacgattacg atcggcgtat caccttctgc
     7021 taacacaagc agcccgttga taatcctatc tccatctttc aaattcatag acttcactcc
     7081 agctgccttt gctccaacaa cgggaacatc tgttaaagga tagcgcaaac ccattccttg
     7141 atgcgttact aagaaaacat ctaagcctgg ctgatcttct ttcagataca cagcaatcag
     7201 ttcatcttct tccgccttta gcttcataca gctgagcgga cggctcttat aggtgcgcca
     7261 tggttcgaac tctgtcattc gtgtttgctt gatcaatcca ttcttgctga tgaatacaaa
     7321 tgttttctct gcttgaattt tttgatatgg gaaaacagct aaaatcgatt cgtctacaga
     7381 cagattggag attgtttgag aaatgtgctc accagcatct ttccatttca aatcgggtag
     7441 ttcatggaca gggcggtaga tcacatttgc tttatttgtg atcagaagaa catggtccaa
     7501 tgtactcaat tcacctgagt atagcaagta gtcgccttct cgcatcccga tttcttctgg
     7561 tttggaagca ctataagaac gaaggcttgt tcgcttcaca tagccttctc gcgtcacact
     7621 gacaattacg tcttcttgtg ccactaagac ttgcgtatca attttgattt cttcgatttc
     7681 gtcttcgatt gtcgttagac gagcggaaga ataatttttt ttcacttctc gcaattcttt
     7741 tttcataaca gagaaaagct ctttgtcatt tgaaaggatt ttattcaact ctgtgatttg
     7801 cgcaatcaag ctttcggatt ccttttgaag ctctgtgata tccgtgttcg tcaaacggta
     7861 taattgcaat gtgacaattg cttctgcttg ttcttctgta aattgaaaga cgtcgactaa
     7921 attctttttg gcgtcttttt tgtctttact ttcacgaatc gttgcgatta cttcatccag
     7981 aatcgataaa gctttcatca atccttctac gatatgctgt cttttttgag ctttggctaa
     8041 atcgaattga ctacgttttg ttatgacttg ctttcgatgc tcgatataac ttcttaaaat
     8101 gtctttcagt ccaacttgat gcggagtcat atggtcgata gcgaccatat tgaaattgta
     8161 attgatctgt agctcagtgt ttttgaacaa ataattcaaa attccttgag cattggcgtc
     8221 ttttttcaat tcgacgacaa tttgcagacc tgttctatca ctttcatctc gtacttcagc
     8281 tattccatcg attttcttat tcaaacggat ctcatccatt tttttcacca aggtcgcttt
     8341 atttacttca tatgggattt ctgtgatcac gatttgctgt ttattaccct tcattgcctc
     8401 tatctttgtt ttcgaacgaa gaataacttt tcctcgtcct gtttcataag cttttttgat
     8461 ttcatctttt ccttgcaaga tccctcctgt tggaaagtca ggtccaggaa tatattccat
     8521 caatttttca aggctcgctt gtggatgatc gatcatatag attgtgccat cgatcacttc
     8581 agctaagtta tgagtcggga tttctgttgc atatccagca gatatacctg tcgatccatt
     8641 gacgagaagg tttgggtatc gagctggaag gactgttggt tctttttctg tatcatcaaa
     8701 attccacacg agatcgactg tttctttctc gatatctgct agcatctctc cacttaactt
     8761 ggataatctg gcttcagtat aacgcatcgc agcaggtgga tcaccgtcca tactcccgtt
     8821 attcccgtgc atttcgatca gtacttcccg caacttccag tcttggctca tgcggaccat
     8881 cgcttcataa atactgatgt caccatgagg atgataattc cccatgatat ttccgacaga
     8941 ttttgctgat ttacggaatc ctttgtcata cgtattgcca tctttgttca tggcaaataa
     9001 tatccggcgt tgcactggct tcaatccatc acggatatca ggcagtgcac gttcttggat
     9061 aatatatttg gaataacgtc cgaagcggtc acccatcact tcttctaaag ttaattcttg
     9121 tacttcttgt cgattttcca tcaggtgacc tcctattcta cttcaaataa ctgattgttt
     9181 tcatttttat ctgctcgttc ttcatctaat aaatcattag aaactgaagg agagatttca
     9241 gtatcctctt ttttatctaa gatactgcca tcttcttcga gcgtaaattg cacgtgattt
     9301 tcgatccatt ttcttcgagg ttctactttg tcacccatca gtgtggttac tcgacgttca
     9361 gcttgcgcag catcatcgat acggacgcgg atcaacgtac gggaagtcgg atccatcgtt
     9421 gtttcccaca attgttccgc attcatctct ccaagtcctt tatatcgctg cagcatatat
     9481 cctttgccga cttttttaat cattgctgcc aattcgtcat ctgtccacgc atattcaatg
     9541 acttgcttct ttccttgtcc tttggatact ttatataaag gcggtaacgc gatatatact
     9601 ttcccagctt caatcagcgg tttcatataa cgataaaaga aagtaagcag taatacttga
     9661 atatgggcgc cgtctgtatc cgcatcggtc atgatgatta ctttgtcgta attacagtct
     9721 tcgatagaaa actcaggacc gactcctgca ccaatcgtat agatcatcgt attgatttct
     9781 tcatttttca agatatcttg cattttagct ttttcggtat tgatgacttt tcctcgtaaa
     9841 ggtagaattg cttggaactt tctgtctctt ccttgtttag cagatccgcc agcagaatcc
     9901 ccttcgacta aatacaattc atttttctta gggtttcggg attgtgcggg tgtcaatttt
     9961 cctgaaagaa gagattcgcc ttttttacgc ttcttcccat ttcggctttc ttctctggct
    10021 ttacgtgctg cttctctcgc ttctctggct ttgattgctt tacggaccaa catctggctc
    10081 atttcgctat tttcctgcaa ataaaagccc atttgttcac tgatgacgtt atctactacg
    10141 gtacgagcaa ctggcgtacc aagtttttct tttgtttgcc cttcaaattg cagaagattt
    10201 tcaggtacac ggatagaaag tacagcagct aacccttctc tgaaatcact accttccaga
    10261 ttcttgtccc gttctttcag caaaccaact tttctcgcat attcattata agatttcgtc
    10321 atcgccgctt tcataccagc ttcatgggtt cccccatctt ttgtccgaac gttattgaca
    10381 aaagataaaa cattttctga ataaccgtca ttgtattgat aagcgacctc tacttcgata
    10441 ccttcttttt ctcctgaaaa ataaacaact ggtgtcaatg tatctttttc ttcattcaga
    10501 taatcaacaa attctttgat tccttcttcg taatgaaaaa tttccttgac tggttcttct
    10561 ccacgaagat cagaaagaga gattttaacg ccttttagaa gaaaggcaga ttctcttaac
    10621 cgttcggcta aaatttcata tgaaaatttc gttgtactga atattgtatc atctggtaaa
    10681 aaatggacag tcgtcccgtt tgcttttttt gtttttccta tctttttcaa agtaccgtca
    10741 ggtttaccgc cgtttttaaa tttctgctga tactctacgc catctctaac gatcgttaca
    10801 attagccatt tcgataaggc atttaccaca ctggctccta caccgtgcag tccaccagaa
    10861 gttttgtacc ctccttgacc aaactttccg ccagcatgca gcacagtaaa aatcacttca
    10921 actgttggta ttccggaagc atgcattcct acaggcatcc cacgtccgct atctgcaaca
    10981 gttatactat tgtcttcatg gatggtcaca tcaatctcac tgccataacc tgataatgct
    11041 tcgtcaactg cattatcgac gatttcatag actaaatgat gcaagcctcg gctgtcagtg
    11101 gatccaatgt acatccctgg tctttttcta actgcctcta atccttccaa tacttggatg
    11161 gacgagtcat tgtattcgtt gtttactttt tttgccaagg caaacgctcc ttcaataata
    11221 ctttgcggca aaaccgcacc acgattatca tactagaaaa ggtcgtgaaa aaaaagaggg
    11281 tagacgagaa atcaatttta attatctcct aaccgctttt tcgacattcc cctctatcga
    11341 tcatctcttc tttgtggtca tttttatttt tggttttctt tcaacttacc aagttcaatc
    11401 ttaatacaac gatccattac gatatctgta cgtcctgctt ttcttagcat ctcagctgct
    11461 tctttgtttt caatccctaa ttgtgcccag aacactttag catcagtttt caaaaaatca
    11521 cctgctattt caggtaaaaa ttcactccgt ctaaatacat ctacgatatc gattggatca
    11581 tcgatttctg tcaaattcgc atagactttc tcgcctagaa tttcttcacc agccaataat
    11641 ggatttactg gtatgatttt atatccattg tcttgcaata cctgtgcaac catgtaactt
    11701 gttcgttctg gtttattgct caagcctacg acggcaatat tttttgcttc ttccaaatag
    11761 ttgaaaatcg tttcttgtgt tggatttttt acagacattt ctgccacccc ttgcttttct
    11821 ttttattcca tctattatac ttgtatcttt cattttttca attgttcctg ttacctattc
    11881 atgctaaaat aaaggatagt tttcaacttg aaagagggta aaaaatgaaa ttgatcttat
    11941 tacttatttt agcatatctt ttaggttcta ttccttccgg tgtctggatt ggcaaattgt
    12001 ttttcaaaaa agatatccga caacacggga gcggtaatac tggaacgacc aatacatttc
    12061 gtgtattagg caaaacagcc gggattgttg tcctgcttat ggatatttta aaaggtacat
    12121 tggctacttg tttgccgatg atcttccacc tttctgggat taaccctctt tggtttgggg
    12181 tatgcgccat tcttggccat acatttccga tttttgctgg gttcaaagga ggaaaagctg
    12241 ttgcgacaag tgcaggaatg cttttaggat ttaatcctgt attttttgtt tattcttgta
    12301 tcatctttat cgttagttta ttttgtacaa gtatggtgag tttgagcagt atgatcagtg
    12361 cagtattgat cactttatct acgatcgtcc ttccttatgt agctcctgtg attttggcta
    12421 agcccaattg gttactgacg atcattgcct ttttagtttc ttcctttatc ttttatcggc
    12481 ataaagacaa tatcaagcgt atcagaaacc atactgaaag tcgaatcccg tttggtttga
    12541 atagcagcaa gaaaaaatcc tgatactttt tcattccaaa gagataaaac gggagtgtga
    12601 caaatcgatg tcacactccc ttattactta caggatagct tttttacaca ccttggtaaa
    12661 cggtgttccc tgctaacttc tcggtcacaa cctcttactt cactgtgatg gaatattttg
    12721 tcttgaagat ttcatgggca cctagttgat tgatccctac tttttccatc aagtttccac
    12781 tactgttcac cgtgtcagcg attccgcacc acggttcgat acaaacgaaa ggcgcttctt
    12841 ttggataagg agaccagatc cctacataag gcatatcttt atagcttaaa gttacgctat
    12901 gggaagattt ttcacttcga atggcgtacg catttagacc ccttgtttca aaaaccaagg
    12961 catcttgctc aaaaagttca cgattcaaag ccagattcgt atttgtctga cctaacgtct
    13021 tttgatccat atcgatgaat gggcctttca atggtaggcg taatcttgat ttcaatggag
    13081 aaaatgccag atagaaatct tcaaatgaca atccttcttc aagaggtaca ttgaaagcag
    13141 gatgtccacc gatagaaaag aacatttctt tttcaccggt atttgccact tgataatgaa
    13201 ccgtgatgcc gtctcctcct aattcatagc tgatcactaa ttcaaaatca aatggatact
    13261 tttctaatgt ctccggcgta ctccttaaga tgaaactagc catttcttgt ccatgttcaa
    13321 tgacttcaaa atccatatcc cgagcaaatc cgtgttgccc catttgatat gttttccctt
    13381 gataaacata ctgatcatct tttaaacgtc caacaaaagg gaataaaacc ggtgcatgtc
    13441 tgccccaata cgctggatca ccttgccaga tatactctaa accatgttct tttgatttca
    13501 gactgatcaa ttccgcaccg tgttcagcga tttcggcgat cagctggtca ttttgaataa
    13561 taacagtcat tgccattcct ccaacttcga cttacttttt aaaatgggaa ggctggaaca
    13621 aacatttcta tctgccccag tccccttact tctaataact gtgttttgct aactcgtctt
    13681 taaaacggga attcaatact tttaaataag ttcccttcat gcctaacgaa cgagattcaa
    13741 tgattccagc tgattccaat tttcgtaaag cattgacgat cacagaacga gtgatgccga
    13801 tactatcggc aatattagaa gcagtcagac gaccttcatc tccatccaat gcttcaaaga
    13861 tagcttgtac agccttcaat tcgctataag ataatgtgtt gattgccatt tggacagccg
    13921 ttgcactccg tacatctgct tcgatgcttc gagacttctg ataaaggatc tgcataccga
    13981 caaccgtagc tccatattct gctaagacta gatcgtcatc tgcaaacgat tgttccatac
    14041 gagataagat aatcgtaccc aaacgttcgc cagctccaaa gatcggaacg acagtcgtca
    14101 gaccaaatgg atatttctct cttgactcta ccggaaatgc agtaatatca ctagtaattg
    14161 ggatattagc ttcagttttc atcaactgtg ccactgtatc agtataagat tcaggaaatt
    14221 gtttctcaac aaacattgtt ttgattcgtt cattgttcac atcgtgacgt tcattataac
    14281 ctagtaatac tccgtcactt cctatgatat aggtgttgct gtcaagaata ccacctagtg
    14341 tgacagccat tttatcgtaa ggcaaatctg cttgcatgtc aaaagtattc ttttgctgca
    14401 aaagtgtgtt tatcttgcgg gttttcgtta gcaagtctgt cattgtcgtc ctccgatttt
    14461 ttataaaata tagcgactta aatcttcatt tgccgcaatt gagccaagtt tgtcatccac
    14521 atatgcttct gtgatcgtta tttctcccat ttgcatatct ggtgcttcaa ataatagatc
    14581 ttctagtagt ttttccaaaa tggtatgcag tctgcgtgcg ccgatattat ctgtgtcacg
    14641 gttgacatcg aaagcgattt gagcgatccg ttcaattgct tcttttgtaa acgtaactga
    14701 cacattttct gtaccgatca acgcgacgta ttgttttact aaagcattat ttggttcggt
    14761 cagaatcttc acaaaatctt cagctgtcaa atcatctaat tctacccgta tcgggaagcg
    14821 tccttgaagt tctggaatta gatcacttgg tttagacaaa tggaaagctc ctgaagcaat
    14881 gaataggacg tgatctgttt ggatgatccc gtattttgtg tttacttgtg aaccttcgac
    14941 aattggcaat atgtctcttt ggacgccttc tctggatact tctccagatt gctgtgattt
    15001 agaagtgatc ttatcgattt catcgataaa aataattcca ctttcttccg ctaatttgat
    15061 tgcctcacta tggatatcag catccttgac taattttgct gattcttcct tgatcaataa
    15121 ctctaaagct tctttgactg ttaccgtccg ttccactttt ttcttcggtg tcaaagcacc
    15181 tagagtttca tttaggtcga tccccatttg ctccaatcca ttgttcatca ttggtgcagg
    15241 tttcttttgg tcttcaactt caatggttac ttcacgacta tccagtaaac ccttttgtag
    15301 ttgttccaag actgtacggc gattgattcg aatatcttct gtcacttctt cttgcggttc
    15361 agcggtattt tgctgactag cattaaaaag attcatcatt tgttcaaatt gattagtgga
    15421 ttgtttctgt tcttttttga taccaggtac caagattttt accaaacgtt tttctgcatt
    15481 ttttttagca cttgtgtata cacggctgta ctgctctttt tctacgattt ggatcgcatt
    15541 ttctactaaa tcccgaacca ttgattctac atctcttccg acataaccga cttcagtaaa
    15601 ttttgttgct tcgactttga caaatggtgc attgacgatt tttgccaaac gtcgtgcgat
    15661 ttctgtttta ccgacccctg taggtccgat catcagcata ttttttggtg taatatcttg
    15721 ttgcattttt tcatctaact gcaaacggcg atagcggtta cgcaaagcaa cggcaacaga
    15781 tttttttgct gcgtgctgtc ctacgatata ttcatctaat tcacttacga tttgtcttgg
    15841 tgtttgtttt aattcataca tatctactca cctcttacag ttcttctacg ataatattat
    15901 gattagtaaa gatacagata tctgcggcaa tatttaacgc attttctgca atttctttcg
    15961 cactcatgtc gttttgagca tagtttttca ttgctcgggc tgcagccaac gcaaaattcc
    16021 ctcctgatcc gatagctaag ataccatcat ctggtgtgat aacttctcct gtacctgaaa
    16081 caagaagcat ttctttatca ttcattacga tcagcatcgc ttcaagtttt tgcatagatt
    16141 gctgtgtccg ccattcttga gctaattcga cagctgcgcg ttgtagattc ccgttatatt
    16201 cgtttagttt gccttcaaat ttttcttcta aagtaaacgc atctgcaaca cttccggcga
    16261 aacctacaac aacttctcca ttgtagatac gtcgtacttt ctttgcagtg cctttcatga
    16321 caacagattc acccattgtt acttggccat ctccagccat agcaaatttc ccgtcttttt
    16381 cgacggcaca gatagtggtt gaatgaaatg gtgattcgac catgtttgtt tcctcctaaa
    16441 tttgttcttt tccttatttt tacgctcttg gatggaaaga acgatagttt ttttgtaaac
    16501 tttcttttgt tacatgggca tagatctggg tagtcgataa attggcatgt cctagcaatt
    16561 cttggactgt ccgcaaatct gccccattat ttaataaatg agtagcaaat gtatggcgca
    16621 gcatgtgagg atggatatca ctatccaaac tgcttttttt gatcatttga ttcaagacat
    16681 attcaattcc agtaggcgtg attggttttc catgatgatt cacaaaaacg atatcatgct
    16741 cttcgtgata ttttgtcatc aatacctggc ggccttccct gaaatattct tgcagagcat
    16801 cttgagcaaa agaacctaga ggagcatatc tctctttgtt ccctttacca tggatcaaca
    16861 tgacctctga atcaaaatca atgtctgata ccgacaaact gctacactca cttaaacgga
    16921 tgcctgaccc gtaaaggact tccagtaaag cgcggttcct caaatccaaa ggactatctc
    16981 ctgctacgct gtcaaaaagt gcttgcattt cattttcata aaaaaaacgt ggcagtctca
    17041 aatttttctt tttgagatga acgtaagaaa atggattttc ttttatcact tcttctttga
    17101 gaagatactg ataaaacgaa cgtaggctgg ctatcttgcg actgatagaa tttctgctat
    17161 agctaggacg atgatcagct aatgcactca aatagacacg aaccgtaaaa tattccgcct
    17221 tcagcagatc agcgtctcca gattcctcca aaaaatgttt gaaatggaga atgtcttctt
    17281 catatgcttc tttcgttttt tctgaatatc ctctttcgac aatcaaataa cgcaaaaatt
    17341 tttgttccca cgtggcttct gtcattttct caccttcctt ttgtcccaga gaataattta
    17401 gcacagcgtt tataaaaaga caaataaatt gtcagaattt aaccaatatt tctaagtttg
    17461 ttcacaattg gcgcaattcc tcacattttg tttgagaaat tcttaagaaa aacgcaagaa
    17521 aaagagcgag actatccgtc tcaccctctt catggagttt ccttactcat ttgggattgc
    17581 agttttttct aattctgttt ttattgcctc attagctttt aaagctcgtt gtgcaattgc
    17641 ttcatatctt gcttttttgt ctcgaatgcg ctctggcagt tcaggaaaca gaccatagtt
    17701 ggcattcatc ggttggaaat gttttccttc cgcatgagta atgtagtaag ccatacttcc
    17761 aatagctgtt tcttgtggaa actcgactgg ttccatttct tttgctaagc gtgcggcgtt
    17821 gattccagca agtagtccgc tcgctgcact ttcaacatag ccttctacac cagtcatctg
    17881 acctgcaaag aacagatctt cgcgtttcgt tgattggtaa gtcggtttca gcaattctgg
    17941 cgaattcata aagctgtttc ggtgcatcac accatagcga acgaattcag cattttctaa
    18001 cccaggaatc atccggaaaa cacgtttttg ctcgccccat ttcaagtgag tctgaaagcc
    18061 cacgatgttg tataaagaag ctgcagcatt gtcttgacgc agctggataa cagcataagg
    18121 acgtttgcca gttttaggat cttccaatcc aactggtttc ataggtccga aaagcatcgt
    18181 tttgaatcct cgctgtgcca ttacttcaat tggcatacat ccttcaaaat atttttcttt
    18241 ctcaaaagat ttaagttctg ccacttcggc attgattagt gcctcatgga aagctttgaa
    18301 ttcctcttct gtcatcggac agttcaagta agctgcttct cctttgtcat agcgggactt
    18361 caaatatact ttatccatat cgatcgtagc tttgtctacg attggtgcag ctgcatcata
    18421 gaaataaaaa ccatctgaac cattgaatgc tttgatgctt tctgctaatg gatgagaagt
    18481 caaaggtcct gttgctacta cggtgattcc ttccggtatt tcagtaattt cttcgtttat
    18541 cactgttact aatggatgct ctttgacttt tcgggtaatt gtttcagaaa aagaatcgcg
    18601 gtcaacagct aaggcaccac cagctggcac tgctgtttca tctgccgaag tgattactac
    18661 cgaattcaat cggcgcattt cttctttcaa taaccctact gcattagcca aactgtttcc
    18721 tcttaatgaa ttggaacata ctagttcagc aaaattactt gtgtgatgcg cctcagtcga
    18781 tttgactgga cgcatttcat ataaacggac agggactcct gcttgtgcga tctgccaagc
    18841 agcttcactt ccggcaagcc ctgctccaac aacatttacc attttcaatg aaagaacctc
    18901 ttttctatta tttttggatg tcttcttcgt aatctccatt tacacagact acttgtttac
    18961 cgcctttgac tttcttctcg accagatatc cgtcacattt tggacaattt ctgcccactg
    19021 gtttatccca agaagtgaat tcacattctg ggaaacggga acaaccatag aatagtctgt
    19081 ttttcttaga ttttcgttca atgacttgtc cttcatgaca tactgggcaa gtcacaccga
    19141 tttcttttac gattggtttc gtattccgac aatcaggaaa attactgcaa gcataaaact
    19201 tcccatattt ccctaatttt atcaccatcg gatgtccaca gacatcacaa tcgaatcctg
    19261 ctggttcatc tttgatttga attttttcga ttttttcttc cgcattcgtc agttcttttt
    19321 caaacggttt atagaaacga tcaaccactt tgacccacgc ttccgtgcct tcttcaattt
    19381 tatccaaatc tccttccatt tctgctgtga aatggatatc gacgatttga gggaagaatt
    19441 cacagattaa tgaattgaca atttctccta gttcagtcgg ctcaaatctt ttttgtgtca
    19501 attttacata gtagcgtctt tggatcgtat caagtgttgg tgcataagtg gaaggtcgcc
    19561 ctaccccgtt ttcttctagt gcacgaatca acgtagcctc actaaaacgt gctggtggtt
    19621 gagtaaaatg ttgttttggt tcaatatcaa cagattggac tacatcgcct tcttcaagtt
    19681 ctggaagaat attttctttc tcttctttgc cgtcatctct accttccaca tacacttgca
    19741 tgaatccttt gaatttgatt tttgaaccgt tcgcaatgaa gatgacacca ttttgttcta
    19801 atgtcacttt cattgtatct aaaatagctg gtgtcatctg actagccaca aaacgtgacc
    19861 agatcaatgt ataaagtttc aattgatctt tatctaaata ttgtttgagt tcatctggtg
    19921 tacgaagcac actcgttgga cgcacagctt catgggcatc ttgtgcgcct tgagcatttt
    19981 tgatctttcg gtgatcatgt ctagaaaatt cttttccata cgtatcttca ataaatgctg
    20041 cggcctctgc ttttgctgta tcggcaatcc gggtagaatc agtacgcatg tatgtaatca
    20101 agccgactgt tccttgttta cccaaagtga ttccttcata taattgctgt gctaccatca
    20161 tggtttttct tgttcgaaag tttaacttac gggcagcttc ttgttgcagg ctacttgtag
    20221 tgaaaggatt cgcagggttt cttttgcgct ctttcttttc tactttttta acttcgaagt
    20281 ctgttccatc aattctagca gtgacttctt ttacactttg tgcatctggt aatttctttt
    20341 tcttaccgtc gattccccag aaatttgctt tgaatttctt gcgagctttt ttaaaattac
    20401 cgtcgatgct ccaatattct tctggaacaa attctcggat ctctttttca cggtcaataa
    20461 taatttttaa agcgacagat tggacacgtc ctgcacttaa ccctttcttg accttacgcc
    20521 agagaatagg actgatcgaa taaccaacga ttctgtctaa ggtacgacga gcttgctgtg
    20581 catctactaa atctacatcg atcgttcttg gttccttaaa agctgctttg actgcgtctt
    20641 ttgtgatttc attgaagacc acccgatttt tttctttcag atccaaccca agtaggtacg
    20701 ctaaatgcca agcaatcgct tctccttctc tatccgggtc acttgccaag taaacttttt
    20761 gagctttttt tgctgcggct ttcaggcttt tgatgacatc gccttttccg cgtatagaaa
    20821 tataatgggg ttcataattg ttttcaaaat cgatacccat tttacttttc ggtaaatctc
    20881 ttatatggcc aacactggcc attactttat agtttcgtcc aagatatttc tcaattgtct
    20941 tggcttttgc aggtgattct acaatcacta aatatttata cgccattact gaatggactc
    21001 ctttccattg tcgtgttcct actattaaaa gaggattcat acaaatcgta gctaatcata
    21061 tccattcaga aaacgtttgt caagaaaagc aaatgaaaag tatggtgtta ttctttaaat
    21121 tctgccaaga tatcttcacc cgaaaccgtg caaactgcac cgtcttggat caaatgatga
    21181 catccggtag aatgttctgt taaaatattt cctggaacag aaaaaactgt tcttccaaga
    21241 tccaatgctt gctgtgctgt tatcagagag ccgctttttt tcttggcttc aaagacacat
    21301 gttcctttag acaaagctgc gataatccga tttctcattg gaaaacaatg ctttttcggg
    21361 ccggattgag gaggatattc actcaaaaga agatgatcat ttgcaatcac ctcttgcaag
    21421 tcacgatttt cagcaggata gacttgctct atccctgttc caatgactgc gattgtccgt
    21481 ccaccatgag agattgctgc ttggtgaccg tatgtatcta tccctttcgc gcagccactg
    21541 acgatcacat atccagcttc tatcactggt tttagtaaat aataaactgt ttttttggct
    21601 aaagcagaag tgtgtcttcc accaacaacg cttaacatgt tacaagaaag taaagataga
    21661 tcaccattat aaaacagtac aattggcgga taggtcaagt gaagcagctg agggggatat
    21721 ttgggatcaa agcaagtgat aaacgactgt ttcgattgga ttttgcccca gttagcgtga
    21781 atttcctgcc aacttttgcg aaacagctcc cggtgtttgg ttattttacc tatcttaatg
    21841 atgtcttcaa atccgatttc tttataagga tactgcatca tccagttagc aagtgcccat
    21901 tttccttgac tactgattcc ttttgtataa gcaagtttca ataaattttc ttctatttga
    21961 tacatcataa aaccgccttt cgtctctctt tctaataaga tacgtaaaag cggctgtttt
    22021 ttattgtaaa tattttttta caggtgcaaa acttttacga tggattggcg taattccttg
    22081 tcgttcaagt ccttctaagt gttcctttgt tccataacct gcattatgcg agaaaccata
    22141 tccaggataa agcacgtcat attctttcat caattggtct cgatatactt tagcaatgat
    22201 acttgcagca gcaatcgaca cagatcgtgc atcccctttg attatttttt cctgctgaat
    22261 aggcaaatcc aaggtcatcg catcgatcaa caaatagctt ggtacaatgg acaatttttc
    22321 tactgcttgg atcatcgcta atttacttgc ttcataaata ttcacatcat cgattgtctg
    22381 ctgatcaacg atcccgatcc cgattgccag ggctttttct tgaacttgtt gataaagaag
    22441 ttctcgcttt ttctctgata attgtttgga gtcattcagc cctaatatga tctcattttt
    22501 cggcaagata actgcagctg ccacgacagg tccagctaat ggtcctcttc ctacttcatc
    22561 aatgccggca atccattggt gaccttgtgc gtacccttgc ttttcaaagc aaaacatttc
    22621 ttcatatttt tccttaatta atgcttcctt tttcaatcgt cgttcaaaag aagccagtgc
    22681 ttgctgcacg cctttgcgtg gatcatttct taatgattct agtgtaggat catctattct
    22741 ctgtatgctt ttcaatcgtt ctttgatact ttggatggat tcagtcatca cttttttgct
    22801 ccatttcttc gcaacgatcc agtgtgtatc gtccaagttt tccttgacga atatcatgca
    22861 cgactaattc gctgccacga tcatagtctt ctttataccc acgttttgct gtaatatcca
    22921 tcagtaattc cggtgccaat ttttcttctt gctcgattga taaaccataa cgatcgctga
    22981 ttcttcctga ataatatcgg gcaaaaaagt ctaatccgta aatcgctaaa tcatctaaat
    23041 ggacaagctg gtctttgatc gcgcctgtca gcgctagttt tttgccgatt tcttcatctt
    23101 caaatttggg ccataaaatc cctggtgtgt ctaacaattc caactcatta cctaaacgaa
    23161 gccactgctg accttttgtt acgccaggct tatttcctgt ctgtgcaatt ttcttgccta
    23221 ctagtcgatt catcaacgta gatttcccaa catttggaat acctaagacc attgcccgaa
    23281 tagcgcgagg cttgatcccc cgagcagcat ctcttgcccg tttttctttc aatgcttctt
    23341 tagccgcagg tactagacct ttaattcctt tactttcttg tgccgtgata gctaatgcaa
    23401 gatggccttg tttacgaaaa tattcttgcc acagctttgt ctgttgtgga tctgccagat
    23461 ccgctttatt caataaaaca aggcgaggtt tttgctgaat gatctgatct agcattggat
    23521 tacgagaaga taaaggtaat cgtgcgtcta ccaattcaaa tacgatgtcc acatatttca
    23581 ttttttcaga aacttctcgt cgggcttttg ccatatgtcc cggaaaccat tggatggtca
    23641 ttgttctctc cattcctttc ataccggaat attccccggc tttacttgtt tttttctttt
    23701 tcaatataaa ttcacttttt tattataaag aaattccatc tattagttaa gtgaaagtga
    23761 accagtcctc attctatcac atcttcactt ttttgatgat gcaaattttt attttggttt
    23821 ctatatatga atctaaattt ttgacagcgt ttcctaaata agagatgata gacgaacaaa
    23881 acagaaagga gacgaaacaa atgaatagta tggtttttgt taattttcct gttgctgatg
    23941 tcaatcgttc cgttgctttt tatgaaaagc tgggttttaa aaaaaatgaa gaattttcta
    24001 ctgaagaagc tggttcaatg gtatgggatg acactttttg gataatgctt ctctctcatg
    24061 atttttataa aaaatttata aaagataaag aaatagctga taataaaaaa acaagtggtg
    24121 ctttgatttc gtttagtgtt gaaaatgcgg atgaagtaaa aaaatttgct caaattgcaa
    24181 aagaaaatgg tggtactttc cacgaagtag atatgggaat gcctgaagaa gaaatgtttt
    24241 cattagaagt ccaagatcta gacggaaata cattagaacc cgtatggatg aagatgtaag
    24301 cagataacta atctgacgtt ttttataaca aaaaaacaaa ggcggatgat acgataagta
    24361 ttctccgcct ctattttttt tattcgtaca atctcttttt tacagtttat aaaagcagtc
    24421 ttctcaccct ttttttaatt gttagatgcc agtgtatgta aatcaccaat gtgtcatgac
    24481 acattggtga ttttatggta tccttttatt attaactgac aaaggagaga atattttgaa
    24541 cactttgatt gcaaaagacg tttttagatt gcccgaaatg aatgcaagat tgttgtcgac
    24601 ctctgctctt ttattagccc ttgatgtcat cttatacaag atagcgattg gtcctgctta
    24661 ttttcaaatc acattcggat ttttaagtat ggcgcttatg ggttatctat atggaccgat
    24721 ctgggctggt ttgcttgaaa tgttgtcgaa cactttaaat ttcacgatat ttggttcagg
    24781 aacgtttcaa gctgcttttc ttatcacggc tttccttggt ggatttatta atggcttatt
    24841 tttatatcat aaaaagataa acctgccttc cgtcatcgct gcccaattat tgattatgat
    24901 cccagtcagc ctttttatca attcttggct gatgtctgtt ttgtttggtg cagatttcaa
    24961 agtaattttt ctaggtagat tcatcaggaa tatgatccaa atccctattc aaataatggt
    25021 tgtttattca tttatcaaaa ctttagaggc tagaaaagtg tttaaacgag ctgtttgatt
    25081 ttacgtacta aggtagtaaa ctgattttgg attaaacaat aaagcgtgca gtctcaacag
    25141 actgcacgct ttattgtttc tcactggatc cattttattt catcaagtgg ataatagaca
    25201 aatcttgctt tacctagaat atcagtttta ttgatcgtgc caaacgaacg actatctttt
    25261 gaaactcttc gattatctcc aagtacaaaa taattatctt ccggaagctt ggattgtccc
    25321 gttagctctt gtagtgtaaa gtccgtcgta taggatgcat ttgcatgatc tgatttcagg
    25381 tttttagtta gataaggttc cttaactact ttcccattga tcttcaattg atcattttgg
    25441 tagctgacat tttctcctgg aagaccgatt actcgtttga tataaatcgt gccatctgct
    25501 aattgaaaaa caacaatatc aaatcgtctg atctccgaaa atttttccat cacaaccata
    25561 tcaccttgct tcaacacgtt ttccattgaa ttgccttcca ctggtaccgg gatgaataaa
    25621 aatccccgca acatgaacgc aaggaataca gaaacaagta agtatttgaa tacaagccag
    25681 aagcgattaa tatagcgttg tttttttgtc aatctctatc aatccatccc tctttttttc
    25741 attctttctt tattataaga aaaatatcaa aaaaatgcga ctagatagct gaaataaaaa
    25801 taattctttc aattaggcta ttggacaaaa aacttttttg atcataaagt acaaaactat
    25861 ctcttatatg tcatgattat tttctaaata tttttcccat gcttcgtcaa agatatccat
    25921 ggacggcaaa tagtcgacat tcagttccag ataattagaa atttcttcgt agctagagct
    25981 gtgcttagga aattggatgt ctttaccggc ttctgtagcg aaaacagata aagcatcatg
    26041 tgtgccaccg cctcgcagtg tcattaaata atgaaaaaag cttcgattca cccttcgttc
    26101 ctccaatttt cttcgataaa ggcagcccgg cgtgcatggt ttcttttaaa attttcagga
    26161 tgctttttat aatactcttg atggtcttct tctgctagat aaaatggttg ggctggttcg
    26221 atttttgtaa cgattggctg ttcgaaacgt ccactttcag ctaactgttt cttacttttt
    26281 tcagcaatga tccgctgttc ttctgtcaaa taaaagatca ccgggcgata attgtcaccg
    26341 cgatcctcga attgtccgaa agcgtcagtt ggatcagttt gctgccagta aagttctact
    26401 aattgttcat atgaaatcaa tgttggatca tagataatct ccactgcttc tgtatgtcca
    26461 gtcgtttggc tttttacttg ttcataagtc ggatctggta catgccctcc agtatatcct
    26521 gacatcactg tatggatacc aggaagcgtg tcgaaaggct ggatcataca ccagaagcag
    26581 cctcctgcaa atattgctcg ttccattatg attccccctc taatactact catccgatga
    26641 ttttttattt tcttttgtct tgctttcagt actgctggtg ctagttttgt tggtatttga
    26701 gttgtttgaa gcatcagagg tatcttttgg caaatataga ctaaaccgaa tgtcatcatt
    26761 tactaagtcg attttatctg ctttaatata catcccgttt tgcattcgga attgatccaa
    26821 cctaagcatg atagtcagat catctgtatt gacttcgatc caatctggta atttatagtt
    26881 tctcttgatc atattcatca catctttgat tggcaaactc aatgttccga tagaaagact
    26941 tctagctttt agttggacat ttccgttttc catcacatac ggatcaaaat ataaatagaa
    27001 atcgatagga aaacctagta cttcgaaagt accattgagt agtgcttcat tttccaaata
    27061 aaatttatat tcaatatcac tatctttttg atactcacta aggaaaaagc tgatgatttg
    27121 attgactttt tctttatttg aattgatcgt taagacagga tctccttgtc tttccgtaag
    27181 agctggtatt tctgggagtt ctggttcccg attagcaaaa atacggctgc ctaaaaatac
    27241 ggtcgaacca agtactaccc caagaaggat aaggaaggcg aatttccagt aatttgtttt
    27301 ctttgttttg tttgaagata cgtcagatct tgttttcatg tttattgatc atcctccttc
    27361 tctaaccatt ggttttgtgt ctcgatcagc tgatctctca ctgcatttgc catcaattga
    27421 taacctaaat tatttggatg gaaatgatct tcttcataga gcacattatt tttgatgcca
    27481 tttagatttt ctgtcgtgct agttgtttga tctgttcctt cttctgtgac acctacttcc
    27541 tgattcaatc cttgataaag caaatcattg attgggataa agtagcagtt cggcattttt
    27601 tttgtcaccg tttctgttcc tttgttccaa ttgttaacaa ttgtctgcat gtcagtgatc
    27661 tcaggaaagt tcaaataaaa tggattgtag atgcctaaaa tgtaaatagg tgcggtttta
    27721 ttgagggatc gaaattcttc caatagttct gtgacatttt cctgatagtt tttcagtggc
    27781 tttttaaagg aattgaccgt taacccgaat aaattatttt ggataacttt catcaaatca
    27841 ttaccgccaa cagtcagtgt gataaaatca gctgttttga tattgtttcg aatttcttct
    27901 tttgttttta ctcgctttaa gatctggtca cttcgttcac catttacacc atagttttct
    27961 gtttcaaccg cggtcaaatt ataacggtct tttagatcat ttgcgacaag gggaacgaat
    28021 cctccttgct tggtttgatc tcctactcct tcagtcagcg aatctccgat agctatgtag
    28081 tgaagttttt cttttttatt tggatcttct ctcacagtag ttactggttt cattagtggg
    28141 ccagctttcg gtatagaagc agctaaacct gcatagatga tgatcgcaat taataatggg
    28201 aacaaaacga ggatcaaatg tcgtttcgtt aatttcattt tttcacctct tgtattcgat
    28261 tggttctaat gttatcaaaa attttatcaa aaaggtagtc tttatgaaaa aagaaaacaa
    28321 ttattttcct aattttataa agaaaaagag aataagctgc aagcctttca ggctgagggc
    28381 ttatcctctt ttcgactaat ctacataata catgattgca aacgcgtctt taccagtatg
    28441 cgtagcgata accggattcg tatgcagtac agggatatgc atgtccggga ataattcttg
    28501 caaaccctct ttgaattctt gcgccaattc ttttgcatcc gcatgtgaaa taccgatttg
    28561 tcgcacattt ggcaattctt tcagttcatt tttcaaatta ttaaaccatt tatcgaacgt
    28621 tttctttcct cgccccttag ttacagggat taattcagaa tgggcgaaat tcatcaccac
    28681 gcgtatgttc aaaacactag acagcaaacc agttgttcgg ctgattcttc caccttttac
    28741 caagttatct agtgtggata ttccgatgta taattttgta ttgtcttgta cttttttgac
    28801 ttcttcaagg attttctcta ctgttttacc ttcttttgct aattcagctg ctcgaattac
    28861 ctgaaaagat aatccttggt cagtggtatc actgtcgata accgttactt tcgtttttgt
    28921 tagattactg gcttgtcttg ctgcttctac tgttccactc aagccttttg tcatatggat
    28981 cgaaatcact tcactgccgt cttctcctag ctcatcatac atcgagatga attctccaat
    29041 tggtggttgg cttgttttgg gcagattttt tgctttcgcc atcatttcca taaatctttc
    29101 gccgtctagt tggtcatcat cgggatatac tgttccgtcg atcatgatgg atagcggcat
    29161 gacacgaatg cctaaagcat cgcgtacatt tttttccatc gtacatgaag aatctgttac
    29221 gatttttaca tttgtcatta ttttctctct ctctttcttt aacaagatgc cttattcatg
    29281 ttaaactaca tgtaagacaa tttctatttt tagtataaca gacaaataat gatttttcat
    29341 taattacacc tagtaaagga gccgttttca tggaaaaaac gaaatttaat cgaacgtatg
    29401 aaatattaaa tgaagtgttt aatgcagtca cccatggaat tgggactgga ttgagtatcg
    29461 cgggactagt ggttctttta gtaaaaggtg cccacttgca ttcctccctc catattgtct
    29521 cttatgctat ctatggatcg atgatgattc ttttattttt aacgtctact ttgtttcatt
    29581 cattgatttt tacaaaagca aaaaaggtgt tccaagtatt cgatcatagt tcgatttttc
    29641 ttttgattgc aggaagttat actccttttt gtttgctgag tatccgagga tggctaggtt
    29701 ggactttatt tgtattaatc tggttgtttg cgattcttgg cattatatat aaagcgttga
    29761 cgttgcataa acgggagaga gtatcaaaag tatctacgat catctatatt tttatggggt
    29821 ggctctgtgt cgtggctgct gttcctcttt atcactctct tggaatgact ggtgttattt
    29881 tgatggtagc aggcgggctt tcttacacga taggcgcatt cttttatagc ttgaattcta
    29941 tccgctatat gcatgttgtt tggcatttgt ttgttatgtt aggtgcaggt tttatgtact
    30001 tctctgtttt attctttact taataaaaaa aataaagcta tggatgggac agctctaaat
    30061 aacagctagt ctatccatag cttttttata tcgtcaattc gcctgtcccg ctttttacag
    30121 gattctcatt acgctgatac gtttcaaatt gatgcggata acgatttttt tcatccattt
    30181 ctccttgact agtattgatc agtgaccaat cagaccagtc aacaggtgga aaataagcat
    30241 ctccatcgaa tacatgatgg atgactgtac ggtataatat cgtacaaaat ggcagaaatt
    30301 cttgataaac agctgaccct ccagcgatga aaaagatacc gtctgtttct ttttccagag
    30361 caagcacgtc ttccacacta tgcatgacat gcacgccttc tgctttatag gaagaatctc
    30421 tcgtcaaaac aatcgtctga cgattcggca acggacggct acccatccct tcgaatgttt
    30481 ttcgtcccat cactagtgta tttgcttctg tcatttgttt gaagaatttc agatcatttg
    30541 gcaaacgcca aggaagacgg tcttcttttc caatcaatcc gttttcatcc tgtgcccaaa
    30601 ttgcagctaa cattttctca tcctttctga ttctaaacag cgataggagc tttgatcgcc
    30661 ggatgtgggt catatccttc taattggata tcttccatct caaaatcaaa tactgactgc
    30721 ttttcctgat tcaaaactaa agtcggaaat gcgcgtattt ctcggctcaa ttgttctttc
    30781 atttgttcga tatgattgat ataaagatga gcatctccta atgtgtgaac aaattcgcca
    30841 accgctaaac ctgtttcatg tgcaattagg tgagtcagca acgcataact tgcaatgttg
    30901 aacggaacgc ctagaaacac atccccactg cgttgataca attggcagct tagccgtccc
    30961 tcttgaacat aaaattgaaa catcgtatga caaggaggca aagccatgct aggaacatct
    31021 tccggattcc atgcagaaac gatcaatctt ctagaatcag gagtcttttt gattgcttcg
    31081 atcacttgtt tgatttgatc gataaagcca ccgtcttttg tttcccagtg acgccattga
    31141 tagccgtaga tgtgtccaag atcgccgtat gtttcagcaa aggaatcatc ggttaggatc
    31201 ttctcgcaaa aaagcttgct ctgcttttta tattcttcat tgaattcagg atctgtcaaa
    31261 catctatggc caaaatcagt catatctggt ccattatagt ctggactttt gatgtagcgt
    31321 tcaaatgccc attcatccca aatatggttt ttattttgta atagatagcg gatattcgta
    31381 tctcctttta aaaaccaaag caattcgctt ttgatcaatc caaaaggtac tcgtttcgtt
    31441 gttaacaaag gaaacccttt ttgcagatca aaccgcattt ggtatccgaa taagcttaat
    31501 gtgcctgtgc ctgttctgtc ttctttacga tgcccttctt ccaacaattt tcttcctaag
    31561 tcaagatacg cttgttccat cctttttcct cctaatcttc tacccattca ctcaagtatt
    31621 cccaccgtgc cattttttct tcaagttgtg cttctgtttc agatacgtcg ttttgcaatt
    31681 cttgtaatcg agtgaaatta tctccttgat gattcatctc ttcttggagc aatgagattt
    31741 tttcttcaag ttctgcaatc tcatcttcaa tcgtttccca ttctttttgt tccatgtaag
    31801 ataatttctt cttttcagca ggttccttgc tctgtatttt tggtttcgct ttttcagcag
    31861 gctgctgggt ctctttttgc ttcagtaaat aactggacat gtttccgtaa taaagttctt
    31921 gttttccgtt tccttggaat actaataact tatctgctac cttgtccaag aaataacggt
    31981 catgggaaac agtgatcaca gcacctggaa aacttctaaa ataatcttcc aaaatcgtca
    32041 atgttgctat gtccaaatca ttggtcggtt catccaataa taaaacatta ggttgctgaa
    32101 tcagcagttt caacaaaaat aaccggcgtt tttctccgcc tgaaagtttg cctatcagtg
    32161 tcccatgcat aaatcttgga aataaaaatc gttcaagtaa ttctgctact ccgatttgac
    32221 tgccatctgc tgtttttgct tcttcagcag cttcttgtaa gtaagcaatc atccgtttat
    32281 ctggtgccat ttcttcattt tcttgtgtat aatacgcaag atgaaccgtt tcaccgatag
    32341 acattgtacc gctatccagg ggaattctgc cagctagaat attaagcaat gtcgattttc
    32401 ccgcaccatt tttaccagta atacctagtc tttcccgaga ttgaatcaat aaatctaaat
    32461 gttctagcag tgtttggtca tttatacggt aactgccatc tttgatttcc agtacttttt
    32521 tccctaaacg ttgtgtcgct aggttcagct ctaaatcatc ttcttggttt acttggtaca
    32581 agttctcctt tagatcttcg aaacggttga tccgtgcttg ctgcttggtg ccacgtgcct
    32641 gtacgccagc cctcatccaa gctaattcct gtttataaag ccgcttgcgc ttttcttctt
    32701 gttctacttc tacacgatca cgttctgctt tttctaagac gtaagcttca taatttcctt
    32761 tatactcgta taattttcca aatgaaagtt cgaagattcg gtttgctact ctatctagaa
    32821 aataacgatc gtgggtaacc atcaatagtg cgccacggta ttgtttcaaa tagttttcca
    32881 accactcgat cgcttcataa tctaaatggt ttgttggttc gtctagtaat agtaaatcgg
    32941 gttcatcaat caacacttgc gccagactaa ctcgtttttt ctgaccacct gataaagtac
    33001 tgattttttt atctaattca ctgattccta atttttgcaa aataatcttt gcgttggtat
    33061 ctgtagtcca agcatcttct ttattcattt gttcttctgc tttagcataa cgtttttgca
    33121 catcagggtc ttctccgttt tcccctagtt cgatcaatgc ttcctcatat gctcgaacag
    33181 tttggatcaa ggggctgttt ccttgaaaga ctgcttggag aacagttaat tcatcggaaa
    33241 attcagctgc ttgagaaaga taaccaatac ggtaatcagt tggataaaac actgtttgcc
    33301 gatcaccgtc gctgctatcg ataccggcaa taatatttaa gagacttgtt ttacccgtcc
    33361 cgttcgtccc aatcaacccg atacggtctt tttcgtggac caaaaaagaa atctggtcaa
    33421 acaaatcttt ttcaccataa gtttttttga ggtcagttac ttttaattct ttcaacatgt
    33481 tccctcctta tcttggatat tgtatcataa aaatagtgaa cttcaggaaa atttttttgg
    33541 tatttgttct ttttgtgttc tcaaaaaaag aacaggaccg attcgatcct gttctgatat
    33601 gttttgtttt ttataaaact tgaaaatttt taccttttta ttttattgga ctcgtttttt
    33661 ttgactttgc tcgaaacgca aatcggaaag aaaagttatc agatggtttg ttcatcgtat
    33721 aagattctaa caaattgata ttcctgcgat agctgttcgc agaaaacgtc gtgatttttt
    33781 cagcaacttc gtattcatca atgatttttc tttcaagata ataacctctt agcatttttt
    33841 tcgtaaacag actatcttgc tggatcatcc acgaagaaat caaattcccc gagttcattt
    33901 ttattccttc aagtttgcgt tgctggataa aaaattgaac catcgtttca tcataaatat
    33961 cagtcaagtt atctaaactt tcaataatca actgagtatt tttttgatat gtctctctga
    34021 tttcttggat atcttcatca ctaataaatg tctgacgatc agcatatctt ctttgccaaa
    34081 acattttcgg atggagaacg attcgcatta acctacggac aacaatgaac caaaagccga
    34141 taaaagacaa aatctggctt gtgatagaat gttcgaatcg agaaagaaaa cgggagtaaa
    34201 aacgataacc attagaagaa agcttgcccg aacgataact ttcatccaat ccatcccgtt
    34261 cgatcgacaa aatcagtgct tgtatttctt gtacttcttg cttttcatca tcggttaaat
    34321 cttccaagta cagatcacgt atccgttctt gataattttc tatcactgct tcagtggcta
    34381 aaaattcttt tgtattacga tcaatttcct ttatttcttt tcgcaaaact tcaacgacct
    34441 cttctaaaat catcaaagca ttcaaatccg tgcttttcgc ttcctctgtt tccgtcagca
    34501 taggcaagac aatgatccct atgaccaaag tcactaagat gacacccgcc gtcagaaaca
    34561 aaagcaaaga ccgctggtaa aacatcatat tattgattga aaatggcaaa ataaaaatcg
    34621 tagccaaact gactgttccc tttacaccgc caaatgttaa gatcaagatc tcgttcaact
    34681 gttttttgaa ctttttactg ccatctttga atacataaaa aagactgata gaaatgaatc
    34741 tgatcacaaa aagcatgaca gagattaata cgataatcgc taataaaaga ccgtttggat
    34801 ataaaccgtc tccccatact ggcgagaaca cttgcgtcaa ttcgattcct aaaaatataa
    34861 atactaatgc attcagtgta aaagcaatag ttgtccatgt actgtgggac aagctcgaca
    34921 attctgcatc aaacacagag attttcctaa aaccagaagc ttggagaata cctgctgcca
    34981 ctgcagcaat gattccagat acatcaaatg cctctgccaa tacataggct agaaaaggaa
    35041 gcaaaagttc gatcagcaga taccctgtga catcttgtgc tgatgctttt tcgatcaaat
    35101 gaattatttt ttgtttgaac cagacaagca aaaagccgat caaagctccc ccaatactag
    35161 aaactactag actcatcccc gcgtttactg ctgaaaaaga tccggtgatc agcgcaccaa
    35221 gagcaaactg gaaggcagta acaccagatg catcattcaa aagaccttct ccttccaaaa
    35281 tatgcatcgc ctttttaggg atattcactc gtccagaaag agaactgact gctaccgcat
    35341 ctgtcggacc taacgctgct ccaaaagcaa agcaggcagc aagtgggaca cttggcaaca
    35401 ggaatgataa cgtcgcacct accccgacaa gggtcaaaat gactcctcca aatgccaaga
    35461 acaaaattgt cccgaaattt ttgagaatcg aagaaatatc tgctttttct ccttctctaa
    35521 ataacaatgg tgcaataatc ataactaaga acaattcagg ttcaaaatcg attgattgtc
    35581 cccactctgt cagacctaat accactccaa ggaaaatttg gatcagtgga gctggaatca
    35641 ttggcaaaat gcggctagct atgttcgata aggtgatcgt tactgcaaat acaataacta
    35701 actcagcaaa ttccatcgtc catccccttt attttttatt ttgtgtaagc atttttaaaa
    35761 attgttcttt tttctttaaa attttggttt cgactacttc aacctgtgtt tggttcccct
    35821 ctaattcaca ctgtacacgt tttgactcca aacgaagcag atcatgtttc agacgttcca
    35881 actgacgata catcaatata tttcttttac gcaacttaca aagtatttga cggcggtcat
    35941 ccatcgaaag ggcgccatag cgatcatgca acagtttttt ttgttgttca aaactctctc
    36001 tcattttcca gatctcctat acacaaactt ccatcattat agcaaagttc tttttggatg
    36061 aaaaatattt cattgtttca tcgtttattg ctagttagaa atttccataa atttttcaac
    36121 gcctcattct attccaaata gtatcagata tcaagtaaaa cttaagaaag ataaaagttt
    36181 ctgacaaaag gatgtttact ttcccattcc tgcctttctt gttcttttat cacaacaaaa
    36241 aaatggggct gcgacaaaaa tcttgtcaca gtcccttatt ctgaataaac ggtgtggaag
    36301 agacagccta tcagtagtaa taagacacat ctctttcaca gcctcatttt tgcgtaaaaa
    36361 tcgatttatt cgaaaaattc cccgtataca tcttctacac gttgatctgg attgatcacg
    36421 atatacaagt tacctgtttt ttcgctgatt tttgttgatt gatattgatt gtctaatcct
    36481 tctgttcctt tatctagata tgggaagtac acaagatcag gtgtgccatt tcggtaaagg
    36541 atttccattc gctcgatatc ttcgtatttc aatgcgcgat taaacatgcc catttccagt
    36601 ccgcctaggt tgatatcatg tgtcgcaact gcgtcccctt catggaagat ttcgatttta
    36661 aatccagcac acggatggat ttcaacaaat tcactgccat tgattcgtcc aaaacttgtt
    36721 gtgatttgtt tgatccaaag atcgccgata tgacggcggt tgatcgtcca ggtttctcca
    36781 tttctaaaat aaaattttag cgctgtcatt tctttactca ttaaaaagac tcctttatga
    36841 tgtaatttat tcttgtacta cctcagttta gcacaaattt atgtaagtgg tcacatctta
    36901 tgttttttct tctaatgacg tatctgccca ctttaagtag ccaacaaagc tgagcagata
    36961 cagtctagtg ttttttaaat caatttttct ttcgataaaa aatccaaaac ctgtttttta
    37021 tcatttttta ttctttgttc tacgacggct ttttcagcta attgcaaagc ttcactaacc
    37081 cacttaccag gtttttttcc agtttcttct agcaagattt taccatctac tgctaattcg
    37141 cttaatgact tgataggtaa acgatcatag gattctccta cggcatccat ctgaggtgtt
    37201 tcgtcaaaat agatcatcaa ttcttctact tgcagtgcat gttttttgcc taagaagtac
    37261 aattcgaatg gttccaaagc tccagtttct cgttgtcgta gtccttggac aagtgcttga
    37321 acgtctttaa tcatttggtt agaacacttc cacgatttca aaaaggaacg aatctcattt
    37381 tcttttagat ccagttggct gatcaataat gcccatgctt ggctctcttc tttcaatggg
    37441 acggatggta attcagcaaa acgtaaaagt gcttcgcctt cttctttcaa accgggacag
    37501 caaatgtagc actctgtttc aataaatgca tggattcccg cggcccgttc atttcccaag
    37561 agcatcttga tgaattccac atttatacgt tctacagata ttttacctaa tagctcgtgg
    37621 ttttctttga tggcgtagaa ggtttcttcc tctaatttga accctagttg actgacaaaa
    37681 cgcaacccgc gcatcattcg taaggcatct tcatggaacc gctcatgagg attgcctaca
    37741 gctcgcaaga tctgttcttc tagatcttgc agaccatcga acaaatcgat gatttctcca
    37801 tcttctttca aagcaaacgc attgatcgta aaatctcttc ttttcaaatc ttctgccaac
    37861 gaacgaacga attcgacatg atctggtcta cggtagtcct gataagttga ttccgtccga
    37921 aatgtggtga tctcatattg ttcatcattt tctaatacca acacagttcc atgttcgatc
    37981 ccaatatcta tcgtttttgg aaaaagctgt ttgatttctt ctggaaatgc gcttgttgcg
    38041 atatccacat catggattgg ctggttcagc aagatatcac gaacacttcc acctacaaaa
    38101 taagcttcat atcctgcatt ctctaatttt ttcagaactg gaattgcctt ttggtattcc
    38161 agaggtaatt gttttaattt catttttcca acctttcctg cgtgtttctt gtcctgagat
    38221 aaagactcag ttgcatggtg actgtttgac aagtccgttt cagagtcttc aaggccagga
    38281 ccacttcatc ttgtttactc gtccttacgc tcaaagcgat tatggtctct ttgattgaat
    38341 ttttccatgt ttttttcaaa aacttcagtt aagtcgatat tcaatgaatt agccatgatc
    38401 atcgtaacga ataaaacatc accaagttct tctgctaccg agttaactgg ttcagaagcc
    38461 tttttgcttt tttctccata agtgtggttg acttctcttg ccaattcacc tacttcttca
    38521 gttaatcgag ccatctgagc taatggagag aaatacccca ccttgaattg ctggatatat
    38581 tcatccactt cctgctgcat actgtataaa gaacgctttt tatccatctt tttccctcca
    38641 aaactatctt accaaaactt taacgcaaat cctagataag agcaattgag aaaatcgaat
    38701 ttccatgcta catttaggaa ggaatttcta aatggaaatt ctgtgaaaat acataattga
    38761 aagctggaat tcatatggtc aaacgaaaaa cagtaaaaga agttcttttt atcattatag
    38821 gaacaagtat ttatgcattt ggtcttgttt acttgaacat cgctaatcat ttagcagaag
    38881 ggggagtctc agggatcacc ttgatcttaa gagctttatt tggtattgat ccagcttata
    38941 cgactttatt aatcaacatc ccattgattt tactaggagg aaagatcctc ggtaaacgtt
    39001 ccttggccta tactgttcta ggtacacttt ctttgtcatt ctttttatgg atctggcaaa
    39061 gggttccact agaaatcaat ttacagcatg atctactgat cgtttctcta cttgcgggtt
    39121 tagttgcagg tgtaggaagc gggatcgtct accgcatggg tggaacaaca ggcggcagtg
    39181 atattatcgc tcggatttta gaacaaaatt acggcatcac tatggggcgt tccctattgg
    39241 catttgatat catcgtctta cttgcttcat taacctatat cgacttgaaa cgtatgatgt
    39301 atacgttgat tgtttcgtat gttttcagtc gtgtgattga ttcgatctta gatggcggat
    39361 attctgcaaa aggtctactt gtcgtctcca acaaaagcga agagattgca ccactactga
    39421 tgaccggtct gcaaagaggt gtgactttcc tttctggaga aggcgcattc tctggtaatt
    39481 caaaaaaaat catctatatg gtcgttagct ctagtgaatt aactgaagct aagaggatcg
    39541 tccacgagat tgacgaaaaa gctttccttt caatcatcaa tgtgcatgaa gtggaagggc
    39601 aaggctttac ttatttgaag ccacagacaa gattactgaa aaaactataa gaaaaaagac
    39661 aaagataggc tatgaggaaa gattccacat ccagctgacc tttgtctttt ttagtactat
    39721 actattttta actaattgtt atgttcactt acccggttac ttttcttttg tgtctgcaaa
    39781 aggatctttc ggttaacttc atcagataat ttcaaaaaag catccctatc tccatcttct
    39841 agtgcttgat cgatttgagc ataaagcatt tttagttttg cctcttcttc tttttcttga
    39901 aaatatcgtt caattaattc agtatcttct tcactgactg aagaatccca agaagaaaat
    39961 ggattgtctt ctaagataga tagatattct cttgtctgcc aagcattttc gaatatacat
    40021 tccacatata aaggcttttt ccaattcaaa cggatttcat gaaaaatctg gtctgtgtcc
    40081 gtaaattctt ttccctgcaa aaacaatttc atgggttccc cttcaacaga tagatcactg
    40141 atctgcagtc ctcgatccgc aaaattcgcc ccttcaacaa agtggacatt gcttaaaatc
    40201 gcttcatgat tggcaagata attcaagatc caaaaaactt ctcttcggct aaaagataca
    40261 tgattgacca tccacgttaa aaaattcttc ttttcccgta catcaattag catttttctc
    40321 acctctctgt taggtcatct aataatgaca gtgcctccat atctcctggt tcaagtgtta
    40381 aataataagt caataattct tgcgctcttt tcagctgccc ttcttctctt aagaaaaagg
    40441 catactcttt caagaaatct ggttcatgtg atagttcatg ataagcctgt tcatagtgta
    40501 cagctgcaac ggtaaagtct tctaattcat tataagcatg ggctaggttc cattctgcat
    40561 aaggattagc agtttcttcc atctgattga tcacttgtat cacatcttca tatctttcat
    40621 catccaagta aagattactt aaagtaagta atgtttcatc ttgtttgtct cctacttcta
    40681 aagctttcag taaaagttct tctgctcgct gtctatcatg cagacggaaa gcattttccg
    40741 aggccaggtg ataaagttcg acttggtaag gattttcttt gattccttct tctaacactg
    40801 tttgtgcttc ttcaatcatt tcttcttctt gaagagcttg tcctagatat agatataacg
    40861 attgataatg cggattaact tctcgaagtt cttccagata gttgattgcc tgctgatttt
    40921 ctttcaattg aagattcgta aaagctagct ggaacagtcg atcatctgtc ctttcttctt
    40981 tcagtgcttc ttctaaaggt acgatagctg cttcgaattc tccttgcata cttaaggatt
    41041 gtcctaaacg ttcctgcatc gagattcctg aaatttcatt tatcccgctt gccaacaatg
    41101 tttcataaac cactgctgcc tctttgaaac gatcaactga aaaatacaat tcagctagcg
    41161 caaactggat caatggttca tcaggaagca acccagctgc ttctttcaat tttgcttcac
    41221 tcacttcagg gattccgata acttggtaca aatcagctat cgccaataag ctttgcggat
    41281 agtaatcact ttgcttatcc actttttcca ggtattcaaa agctaagtcg atctcattat
    41341 cttcgattgc aatttctgct aacgggatgt tcagtccgtc attttctggg tattggttga
    41401 tcagttgttc aaaaatctgt tttgcttcga tcaaaaagcc aatagccagc aattcttcgc
    41461 ctagttcagc taatatatct gctgggtctt cttttaatgc tttttctaaa aagatttgtg
    41521 cttgtgacaa gtcttcatct gataaagctt gcaacatttt ttcgctattt gtattcattt
    41581 caattccctc ttatctcact gacgatttgt tgaaataccg tcattgttct caataattct
    41641 ttttcgtttg gatgttggta tccgtctatt atacctattt tttctaaaac aagtattttt
    41701 ttgctttttt ctgccaaaaa aagaactttt tccaaatact ccatctttga aatctgttcc
    41761 agccagtgga ttggatagcc aagcaagtcc agccacacaa aaaagttttt tagttggaaa
    41821 tcaaaaggat atgcttggag attccacacg aaatgaaaca tcattcctaa aaattttttc
    41881 atactagcag aaagtagatg gccattttcg gtcagataaa aagcttgttc aaacagttta
    41941 ccataatttg acaggttttc tgcctctaat tgataaaaat agatcatttc ttcgatcatc
    42001 ccggcaaacg gccgagtcgt taattgcttt ttatttggat agtttttata cagattttgc
    42061 aaaaaacgat gatcacaaat cattccgcaa cagataaaag tcaaaaagtc aatctgcttt
    42121 ccgtccacct gccgctcgct gattgtttga tcataaacga tgcgttctgg cagattgatg
    42181 gtttgcagca ctacacgatt tggtagctgc aaaatcgtcc gctgcggtgt taatgctcgg
    42241 cttagagaac ggatcgatac agggatcatc catagtgatg aatcaagggt tgtatgtttt
    42301 tgaaaaaaac ctgccagatc catgatccct tcatttccaa atccgatgat aaacatggat
    42361 tgattttcgg gatagcgttt gacgaaatca agcaggttct ttaattctgt taaatgattg
    42421 cattgagtgt tggcacaaat ataccagtca ttgtttgctt gatttgcaaa taacgggttg
    42481 atcttatctg caaacaaatc ataatagcgc tgattcgtta aaaataagat tggctgatca
    42541 atggaaagtt ctcttagctg actgactagc gtttctccgt aaaacacttt cgtcaacgca
    42601 tcttctactt gataatcaag ttccatacgc atccctcctc ttcttatttt tattttacca
    42661 ttagtaagca aattctagaa aagataagag caaagctcac ctcataactt gcaaataaat
    42721 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
    42781 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
    42841 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
    42901 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
    42961 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
    43021 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
    43081 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
    43141 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
    43201 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
    43261 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
    43321 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
    43381 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
    43441 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
    43501 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
    43561 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
    43621 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
    43681 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
    43741 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
    43801 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
    43861 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
    43921 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
    43981 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
    44041 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
    44101 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
    44161 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
    44221 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
    44281 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
    44341 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
    44401 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
    44461 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
    44521 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
    44581 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
    44641 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
    44701 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
    44761 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
    44821 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
    44881 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
    44941 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
    45001 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
    45061 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
    45121 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
    45181 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
    45241 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
    45301 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
    45361 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
    45421 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
    45481 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
    45541 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
    45601 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
    45661 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
    45721 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
    45781 acttttttag catctaaacg gcgttttgaa agtagatagc tgccgttttc tttatctttg
    45841 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
    45901 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
    45961 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
    46021 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
    46081 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
    46141 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
    46201 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
    46261 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
    46321 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
    46381 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
    46441 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
    46501 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
    46561 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
    46621 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
    46681 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
    46741 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
    46801 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
    46861 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
    46921 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
    46981 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
    47041 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
    47101 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
    47161 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
    47221 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
    47281 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
    47341 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
    47401 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
    47461 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
    47521 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
    47581 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
    47641 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
    47701 aatcgttccc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
    47761 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
    47821 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
    47881 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
    47941 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctc
    48001 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
    48061 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
    48121 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
    48181 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
    48241 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
    48301 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
    48361 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
    48421 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
    48481 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
    48541 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
    48601 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
    48661 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
    48721 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
    48781 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
    48841 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
    48901 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
    48961 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
    49021 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
    49081 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
    49141 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
    49201 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
    49261 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
    49321 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
    49381 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
    49441 aaaggtggat acatctgctt tgttgcagat aaaccgctga ctcatcattg ttttttgcca
    49501 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
    49561 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
    49621 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
    49681 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
    49741 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
    49801 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
    49861 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
    49921 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
    49981 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
    50041 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
    50101 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
    50161 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
    50221 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
    50281 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
    50341 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
    50401 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
    50461 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
    50521 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
    50581 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
    50641 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
    50701 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
    50761 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
    50821 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
    50881 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
    50941 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
    51001 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
    51061 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
    51121 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
    51181 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
    51241 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
    51301 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
    51361 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
    51421 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
    51481 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
    51541 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
    51601 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
    51661 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
    51721 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
    51781 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
    51841 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
    51901 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
    51961 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
    52021 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
    52081 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
    52141 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
    52201 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
    52261 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
    52321 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
    52381 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
    52441 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
    52501 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
    52561 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
    52621 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
    52681 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    52741 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    52801 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    52861 gaaaaaacac tcactgaatg gagctattga cttatccacg tgcttatcga tagccgttta
    52921 tcggtttctt cattcttatg atagaccttg tacctgtatt tgtctaggtt tagaacattg
    52981 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    53041 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    53101 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    53161 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    53221 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    53281 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    53341 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    53401 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    53461 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    53521 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    53581 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    53641 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    53701 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    53761 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    53821 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    53881 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    53941 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    54001 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    54061 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    54121 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    54181 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    54241 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    54301 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    54361 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    54421 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    54481 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    54541 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    54601 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    54661 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    54721 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    54781 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    54841 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    54901 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    54961 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    55021 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    55081 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    55141 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    55201 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    55261 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    55321 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    55381 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    55441 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    55501 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    55561 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    55621 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    55681 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    55741 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    55801 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    55861 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    55921 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    55981 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    56041 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    56101 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    56161 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    56221 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    56281 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    56341 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    56401 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    56461 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    56521 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    56581 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    56641 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    56701 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    56761 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    56821 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    56881 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    56941 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    57001 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    57061 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    57121 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    57181 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    57241 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    57301 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    57361 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    57421 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    57481 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    57541 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    57601 tctgtgacga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    57661 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    57721 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    57781 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    57841 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    57901 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    57961 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    58021 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    58081 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    58141 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    58201 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    58261 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    58321 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    58381 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    58441 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    58501 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    58561 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    58621 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    58681 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    58741 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    58801 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    58861 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    58921 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    58981 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    59041 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    59101 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    59161 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    59221 agacactcag acggactggt ttcttttttc tttttcatag cattcctgac agattccatg
    59281 gaacgttagc cgatgatctt ttactaaaaa atgataacgt tcttctacta cttgctccac
    59341 ttcaagtaaa agatcctctt ctacttcctc gatcgtgccg cagttcaagc atagtaaatg
    59401 atgatggaaa tgtttggcgc cttcttttct caaatcataa cgggctaatc catcattaaa
    59461 acttacttta tcgacaattt tcaattctgt caagatttca agggttcgat aaacagtagc
    59521 aagtccaatt tccggactct tttttttcac taaaaagaag atttcttctg cagataaatg
    59581 ctctttctca ttttccaaaa gcacaagcag agtagcttct ctttgcggtg tcagcttaaa
    59641 ccccgcttca tgtagctgct gttttgtttt tttcatcgcc gaaatagcat tcatgaccat
    59701 ttactcaacc ccaacaattt ataataatta caaataaaaa aagatataac actatgttaa
    59761 ttaaaattat tataagctag ttaagaaaaa catgcaaggg attctcaatt agattttttg
    59821 actagttttg tcacaccgtt ttcttgtgta atcaatcctt gtttcaaaag attacctaat
    59881 gcgcgcttaa atgctccttt gctgatacca aaagctttca tgatttcttc tggatccgat
    59941 ttatctgtaa agttgatttg ctgatctgca gaacgttcta aaatggttaa aatcatttgt
    60001 gcatcatctg atatagcttc atatgctctt ggcttcaatg aaagattcag tacaccgtct
    60061 ggtcggacac caatcactcg accttttatc tgctcgccta gacgaggttc attatatcgt
    60121 tcagaaggat ggataaaccc aagatagaaa tcttccgtta aaacgtacgt acctgttaat
    60181 ttcaagcggt atgctgttcc tatcacatct ttgtttttca tttcttctgt tccatatttg
    60241 cttaaagatt tgaatatttt ttcatctgct aaactgcccc aaatacggtc ttttgcatca
    60301 actctaaggg aaatcatcaa gcgatcccct tttttaggcc atagctctgt cattgtcggc
    60361 aattcatcaa gagatacgac aaagtcttta tctggaagac cgatatcgac aaacgcacct
    60421 aaatcttttc gtgtagcagt aacgattcca aaagcgtaat gaccgatacg gcttttagga
    60481 atttgtgtag tgatccgatt ttcatgtttt tgattttggt aaccgaatcc ttcaactgct
    60541 tcaccgattt tgtgttcacc ttctgatttg cttaagccaa aggtttgtcc gtttttttgg
    60601 acaaagtagt ttttttcatt ttcatcaata ataatacctg taaatatagt tgctaataaa
    60661 tcgttcattt ttttctccct ctatagtgaa aggttttctt aatacatttc tttgctatca
    60721 taacataatg ttatctactt gaatagctga gccttggttt ggaaaggtga aaaaaggagt
    60781 atgacaacag ttgtgtcata ctcccctttt tcgaataaac ggtgttaaac ggcaggctca
    60841 cagcctcttt catttgccta aaattaattg ctcccatttc ctccgaacca tccagtttta
    60901 cagttgatgg ttacgatgta aaatggcaaa gcactttctc caatgccttt catcaatgca
    60961 gattttgttg aagtggcata tagattataa tagccttccc cattttcgat tctggctttt
    61021 tctaatctgt aacctccttc aacgatatat ccacgttgga tacatgtagc aatcactttg
    61081 ctttggacac ctggtgccca cgtccatgct ggattagatg catcggcaat atcacttgct
    61141 ctggcagatt tgattggctg attcgctgag tttgaagagg aaggggtcga ggatgccgct
    61201 tcttgctgtg ctgcttgtgc gaccgcagct tgttgagcgg cttgagctgc agctgctgct
    61261 tgagcggctt gttttgcttg ttcttcagct gcttttttcg cttcttctgc ttttttcttt
    61321 tcctcttctt cttttttctc tttatcagtg agcatcttat ctacttgttt caattgttca
    61381 ttcaactgag cagatagatc tgtatttttc actttttcta ctgcttcttt tgccgtcttg
    61441 tattggtcgc gtgttgctga atccactact tttccgtctt taaagactat agctgttttt
    61501 tctttagctg tttgcaattc agcatattga tttttagctt ctgtttgtgc ttgttgaatc
    61561 aattttccaa aatctgtgtt atccgtttcg attgcttgga tctctttatc tgctttcaac
    61621 ttgatgtcat ctgctagatg atcatcgacg atggcaggtg ctatgaacca ctcattcact
    61681 ttttggatat ctcggatctt agattggata tcttggtaag ttgtttctaa ctctgaatat
    61741 cctttttcat tcttaagact ggataaatca ttttttagtt ttgataaatc ttgatgaatc
    61801 atatctgcac gaataaattg atggtgatca tctgtataaa aggcagcaag tttctcatga
    61861 agttcttcca tttgttgctg tctttttgct tctgctgcta cttcggcttc tactttttga
    61921 tgatggtcat aataagccca gcttcctcca ccgagtagaa gtactgctgc taaaacgaga
    61981 taaggtactt tttttgattt tttcttttgc gctttatctg gatctgtttt ttctaagttt
    62041 gattcttgat gtgttttttc agtatctggc acgattgtta ttgatttcgc ttctgcttca
    62101 aacagcttag gctggttctc ttctttttct atttttgctt caagatcagt accctctttg
    62161 cttacagatt gattttcagc atcggattca ctatttattt catcgggctc tgttgacttt
    62221 ttgtctgtgt tttcgagtat ttcttttttc tctgcttccg gcagtttatt cgttttttcc
    62281 ttttctaatt gtttttcagt aaatacttca gttttttctg tgatatccgt ttttacgtct
    62341 tttattttta ctgtttcttt acctgtggcg ttttctctat cagagtcttt ttcagtttct
    62401 tgttcctttt ctggctctac tttgttttcc tgtgcaattt tttcactgat cattttgagc
    62461 agctctttcg cgtgctcttc ttttgtatct tcacgatgtg ctttgatata agcggatagt
    62521 atcgaatttt cttcctcatt atcgacgatt tctttcagtt cagctactct tcttttttgt
    62581 tttgattcaa gcgtttcttc ttgattcttt cctgattctt ccgaagtctc atgaacagtt
    62641 gctttcttct gttcattttt ctctatatta gttacttgat cgtgagcagg tggttcttcg
    62701 tcttctggtt ctgctttttc gaataattcc attacagatt ctagaggtaa atctttcaat
    62761 tccgaccatt cgattggatc gtttaattct gtatcaggaa agactgcatc ttcagttgat
    62821 ttatcctgca tagctgcatc tgtatttgtt tcttcaattg attcttttat ttgctgatct
    62881 tctgcagtta tttgatgacc gcagtctgga cagatagtat tctgctccat ttttttcgat
    62941 ccgcaatttg gacatttttt catcacttag cgatcggctc ctttactagg tttacgctac
    63001 catttaatct atcatattct atacgtcacg ccaatagctt atttttagga tttacttaaa
    63061 aataatgaat gaatttcctt cctatagaaa aaaaagcttg agactgcgta gtctcaagct
    63121 ttttttcttt taaaataaac caaacagttt tactgatttc tcttctggtc aatagtccac
    63181 gcttattcta agaaatcttt taattgtttt gaacgagaag gatggcgcaa ttttcttaat
    63241 gctttagcct cgatctgacg aatacgttcc cgagtaacac caaatacttt ccctacttct
    63301 tccaatgttc tcgtccggcc atcatctaaa ccaaaacgta agcgaaggac attctcttcc
    63361 cgatcagtca atgtatctag tacatcttcc aattgttctt ttagtaactc ataagctgcg
    63421 tgttctgctg gacttgttgc ttcctggtct tctatgaaat cacctaagtg agagtcatct
    63481 tcttcaccga ttggcgtttc aagggaaaca ggttcttgcg cgattttcaa gatttctcgg
    63541 actttttctg ttggaagatc catttccgca ccgatttctt ctggcgtagg ttctcttcca
    63601 aggtcttgca gcaattgacg ttggatacgt atcaatttat taattgtttc aaccatatga
    63661 actggaatac ggatcgtacg tgcttggtca gcgattgctc gagtgatggc ttgacgaatc
    63721 caccatgtag cataggttga aaacttaaat cctttgcggt agtcaaattt ttcaactgcc
    63781 ttcatcagtc ccatgtttcc ttcttggatc aagtcaagga actgcatgcc tcgtcctaca
    63841 taacgtttag cgatacttac tactaaacga aggtttgctt ctgccagacg ctgtttcgct
    63901 tcttggtcgc cttcttcgat tttcaatgct aacgctactt cttcttccgc agttaaaagg
    63961 gagacacggc caatttcttt cagatacata cgaaccgggt cattgatttt tacaccggtt
    64021 ggcgcagaaa gatcttctgt ttttgcttgt tcagcttttt tctcagcact cttcaagctg
    64081 tgaattgatg gatcaccatt ttcatccacc acactgatac ctgcgtcttc aatcttttgg
    64141 atcaatttgt ccatttcatc tgcattcaaa gtgaatggtg tagcaagctg attggataaa
    64201 tcatcatata caacttgtcg tttgggtttg ttttctttga tgaaagctgc tactgctgct
    64261 tcatacgttt ttttatctgt tccatttgcc ataaaagaag gccccccttg agtagctatt
    64321 tggcttgttt taactgtttc gttaaattga tgatttcaat cgtcaattca agctcaagct
    64381 gtttgtttcc agttcgtctg gcttcttgct gcatttgttt ttttaatgtt atttcatcag
    64441 ctaagccaga tttgctgatc acatctagca agtcttggat ttctcgtggc gaactttctt
    64501 ctgccaccgg taaatattcg atttccacca ccaaccgcct taaattttca tcttgcaaaa
    64561 agtctaaaaa ctctgcaagg ttgaattcat aatgttgtga gacatatgca tcgaataaaa
    64621 aataaacttc tgcatatgca tcatgaataa actgaaattc tcgttctttt aaaatattac
    64681 ggacagaaac ttctttaaat gcacgatata atagaatctg ctctgccttt tctacttgcg
    64741 tcaaaggtct ctttttccgt gcagtttctt gtctcgtaac ctgagtgtgt tgactttcct
    64801 gaggttcttg acgattttgc tgatttgtcc ttcgaagatc gcgcagttgt ttttgtatcg
    64861 tctctctgcc gatctgaaac tctgttgaca gttgagatag atacatatct tgttcgattg
    64921 gtgaaacaac tcggctaagt tcaactagta attcttcgag gtattcgatc ttgtcttttt
    64981 cattatctaa atttttccct tgttttaaat aacgtgattt aaaagtaaag actgtttcgc
    65041 gcccatgagc cactaattct tggaaagatg cttctccata cttacggaga tattcatcag
    65101 gatcaagctt ttctggaacg actacgacag aaagtgctaa tcgactgttt tgccgaagca
    65161 attcaatccc tcgattggtt gcttcaaaac ctgcgttgtc tccgtcatag caaaagacca
    65221 attctttcgt cagcttttcc atttgttgga tctgttgagc tgttagactt gtccccatag
    65281 aagctaatcc agttttgata ccagcctgcc atgcagcaat cacatccata aacccttcga
    65341 acaacaaaat ttctccgttt ttccggatat cgcttcttgc tttatcaaga ttaaataaaa
    65401 cttgtctttt attgaacagt tctgtttctg gactattcaa gtatttgggc tgatcttttt
    65461 cctgcccttt ttcaggtgca agccagcgtc ctgaaaaacc gatcgtctgg ccttgagaat
    65521 tacgaagtgg aaacatcact ctttgataaa aacgatcaga catacggcca tcatcatgcg
    65581 tgacaaacaa tccagagcgt tctaacaatt catttgaaat tgattcattc ttgaatactt
    65641 gctgaagaaa agaacgctca ttaggtgcaa aacctatgtt gaattcttca attaattcta
    65701 aagacaaccc acgactttgc aaatattcta aagctggctg tccagctttt gtatgcaata
    65761 gcatgtgatg atagatttcc gctgcttttt catgagcagc aatcagtttc cccgtagcag
    65821 attcaggatt tcggacagct atagtacgct gacgccattg atcttctaac ggtatctgtt
    65881 cgaagtctgc cacttttacc actgcttctg gaaaacttag accttctagt tcttgaagaa
    65941 aagtaaacac gtttccgcct ttaccacaac caaagcaatg aaaaatctgc ttgtcttctg
    66001 caacagaaaa agacggtgta cgttcttcat gaaaaggaca cagccccaag taatttttac
    66061 cagacttttt caactggaca tattgtccaa tcacctcaac gatattcgtt tgactgcgta
    66121 ctgtttcgat aacctcttga ggaatccgtt gagccatttt ttcaccccct gctctattga
    66181 tatcttttgg gtttaacaga agatatttca ggagaataac cggtaaaaaa tcgcactaac
    66241 tgaaatcagt gcgattttaa aggacacaat tatacatgtt tgattgtatc atatttcatt
    66301 gctttttcaa gtataacaca tttctatctc attaagcatc aatttgctac tcttttcagt
    66361 aactgatttt gaatatcagc cttccagtac aatactcgaa gaacaatatt tacgagcatg
    66421 aatacaaaga agtatcctac gctctctccc aaccctaagt ttacttccaa aattccatgg
    66481 aacagcattg cagctacata tatccctacg atcgtcgtta ctgctaggaa gatgcgcaat
    66541 gggttgaagg gataacaagc ttttacaact gccatgcaac tgatcccaat cagcaagtaa
    66601 tacatcaaag tagtagtttc agcatttccc catgcgaatg tttttccaag tagatacacc
    66661 gcaatgatat tcactactac aagcaaggca tttggtaaag cattaataag tgctgtaggc
    66721 aaataacggt aagtgatttt tcttttatct tgttcaaaag ataaaaagaa ggaaggatac
    66781 ccttcaatcg ctaaatcaat cagcgtgatt tggataggga taaacggaaa tccaattgct
    66841 gtaacggcac atataatcga taaaataaat gaataaatcg ttttgataaa aaagatgcct
    66901 gagactttcg tcacattgtt gaccactcgg cgtccttcaa acaggacttc tggcaatgta
    66961 gtaaaatcag agtccaacaa taccagattc gcaatctgac gtgttgctcc atcaccttct
    67021 gccatagcaa tgctgcaatc tgcttctctt aacgccaaaa catcattcac accgtcgcct
    67081 gtcattgcaa ccgttctgcc gctttctttc aattcattta ccagcaattt cttttgctgc
    67141 ggactcactc gtccaaaaac cgtatagcga tgagcagctt ctcgcacttc catttcttca
    67201 gtaatttgag aaagatcgac atacgcatca taattcggta atcctgcacg tctagcgata
    67261 ttggagacag taaaaggatt gtctccagaa atcactttca gatccacacc ttcctcatga
    67321 agataagcta gtgtttcttg agcgtttttt cggattggat catcgatttc aaacagagca
    67381 accggctcaa caaatgctgg tttttcttcg ttcaattgct gatttggagc gatccccaac
    67441 ataagtacgc gatagccatt cgcttgggct gtttttagac tgtccggaag ccgatcttct
    67501 tccaccaaac gttcaggcgc tcctacgtac acgactccca gttctggaaa ttccatcgcg
    67561 ccccatttgc gatcagaaga aaatgcgact ttgtttgtca catcataccg attagaaagc
    67621 tcaaaataat ctctcatagc ccgcatagtg atgttgttat cactgctttc tgccagatac
    67681 gtccccataa tttctgggat ggctttctcg tacagcggat gtagtgcttc tacccgctgg
    67741 atactcattt tcccttcagt aatggtccct gttttatcca agcataatgt atcgacatgg
    67801 gccagcgttt caattgaata catgtcttga acaaggattc gtttttttgc cagtttggtt
    67861 acccctgtcg ttaatgcaat acttattagc aataccaaac cctttggaag catgcctaac
    67921 aaagctgctg cagatgcaat aacagaaatt ttgatagaag catcgcggat gaacagtgct
    67981 tccaaaaata gtatgatccc caaaggaata atcacgaaac tagtgaattt ggatactttg
    68041 cgaatcgaat ctacaagttc agaacgaata gctttgtgag tctttgcctc gctagtcaag
    68101 cggacagcat aattatctgc acctactcgg ataacttcag ccatgacaga accgcttgtc
    68161 aaaaagcttc cagaaagcaa ttcagctcca gtttctttga caatcaaatc agattcgcct
    68221 gtcaaaagag cttcatttgc ttctgcccgt ccagatataa cctgtaaatc ggctggtatc
    68281 tgttctcctg ctccgatttt taccaaatct cctaatacca gctctttagt agaaatcgat
    68341 tgttctttac cgtcccggat cactagtact tctgattggg aaacaatcga taatttccgt
    68401 acaagatttc ttgcatgtat ttcttgataa attccaataa cgatattgac gacaataatt
    68461 gctaaatacg ccatgttgga atacgcacct acaaataaca gacaaatccc aataccaaag
    68521 ttcagaaaat tgaataacgt caatatatta tcgctgaata tttgttttgt actttttgat
    68581 gcatcttcct ggtagtcatt ttgctggcca gaagccattt tctgaactac ctcttgctgt
    68641 gtaagtccat tcattttctt tttctccatt ttccgccaac tttcaaaaat aatcacgtgt
    68701 ttaagtctag cgtgttccaa gattaaaaac aacttgtttt tataaaattt atagaaaaac
    68761 acacgaatga ttttacaaaa aagatgccaa cctttttgtt tgaatcaccc gtgtgctgtt
    68821 tttattatca atctatcttt acttatattg ttcctccaaa cggctcatcc accaacctag
    68881 tgctgtaccg gctataaaaa gaacaggaca agctagtacc ccagcaactc ccaatcccgc
    68941 gtaatcggta ggaatgatca taatcgttga agcaaaaact acacctaaaa tgaaatggaa
    69001 tagcttgcta tatgcctttg agaaaatgta atccatgatt tttgacaatc caagaacaca
    69061 aagaatccca ccaattccga tcggcacaat cacttcaagt cgtacttctt taaatccgtc
    69121 agccatcgct ttatacaatc ccatatacac caaaaaatta gaagggctca atcctgggac
    69181 gatcataccc aaaccaatca atccgccggc aatcagccat gttccaaaat tctgatcgac
    69241 ttgcccgtca aataattgag cgccgtataa taaaaacaaa taagccagaa caaaagtaac
    69301 aaccatgatg atcacatcag aagtatctct tccttttttc cctgcttctt tccataacgc
    69361 aggaacagtt ccagcgatac atccgacaaa aaaccataat atcgttgttt catacgcacc
    69421 taataaaaaa ctgactaaaa aagaaagtag aaagatgcct gtcactccgc ctagaccaac
    69481 aggcagaaaa aacaaaacat tttctttgaa attttttgta aggtgggcta aaaaagaaat
    69541 cagccgctca tacatgccaa agacagctgc taatgcacca ccgctgactc ctggcaagat
    69601 aaatcctgaa ccgataaaca ttcccttcac aaaccgtaac atccagtctt tggttgtgta
    69661 ccccacttct tgattttcca ttttttgcct tttttccact atttgcgtac tccttctttc
    69721 atttaatttt attcatgatt gaacatttcc tgctatttta cttaaagact ttttcatagg
    69781 tctctattat tttttcattc attttgacaa tatcaggata aaacatgtta cctccattat
    69841 gatagagata gccgccattg taaagcaagg actggatccg ccaatatcgg tattgtttcc
    69901 cttcctcatt tcctaaaagt gggctcagca catctttaga atacgcttct gctaattcta
    69961 ctgtgttttc tccgccatga ttttttacat aggcaatata atctttccca aaattatatg
    70021 cttggacagc tgtccataaa tcgcatcctt gtttctttgc ttctactatc atttccgcca
    70081 aatatttgac accttgatca atactgtcag ctggattatc aatgatctca gcttctccat
    70141 gcagactttc cgagctctgc atgagatctg tcccttttcc ttttgattcg gtcaatatta
    70201 tcgccttgat caaattctcg tagttttcca tatgataatt ttttgcagct gttgttattt
    70261 ctatttgata gctttccact cgttttaccg aacgataagt tcttcctcat aaaaacaaca
    70321 ccgcaccaat aaccgtcaga atgatcatgt aaaaaagaat acggataata gataatcgtt
    70381 ttcgcttcat ttcatcacct agttattttg acatcttttt ttatcttact atttctttat
    70441 cttcttggct atttcttcct tgatttctgt gtacgcctta agaaaaaatg aattttcctg
    70501 tattttttga catcttacga aaatgatcgt tgtttttcag taaaaacaat tgcccaatga
    70561 ctgatataat cgttatactt tacacagtag gtgattatgt gaaaaaagaa aagaaatatg
    70621 caaaaatgcc agatgggagc gaaatttact atgaaaagag cggtcaaggc ttccctctct
    70681 ttttactgca tggcaatgac ggcagcggcc gctttttttc agaacaggtc cctgtactcg
    70741 aacgctacta taccgtgtat ctagtagata gccgaggaca tggtcgttcg acaaacgaag
    70801 catctatgtt gaattttcag ttaatggccg aggatctgaa tacgatcatg ttgctggaaa
    70861 aaatcgatca ggcagatttt cttggattta gtgatggagc aaatctagca ctggtatttg
    70921 ccagcagctt ccctaaaaaa gttcatcgat tgattttaaa ttctggaaac acgcttgtta
    70981 aaggcgttcg tttttctgcc agagtcatca gtaatattca ttatgcatgg gtatggttac
    71041 tctctttatt tcgcccaagt cttcgcaaaa atcttttagt tatcaaactt ttgctgcatg
    71101 acatcgggct tacagaaaat gatttaaaaa aaatcaactc ccccactttg atcatcgtgg
    71161 gaaaaaaaga tgtaatcaag ctaaaacatt ctttgtatat agctaaaact ataccaaaag
    71221 cttcctttgt gcttgtgaaa gaacaaggac atgaattagc tagaaaagat cctgaacgct
    71281 tcaatcgaga agttcttcaa tttctttctg aaacataact attaggaata gaaggtgatt
    71341 tatttgttaa aaaataccat acaatggctg aaaaaccacc taggactgtt caaaacaatc
    71401 tttttgattt ctgtcattgt cattattgtc ggtgaattga tgtccatcgg taaaactcta
    71461 tctatccagc aattaggaga aacgtttgcc actatacctg tatggaaaag cggactaatg
    71521 ctattgattg gtcttatctc tgtgttacct atggttggtt atgatattat tttgaatcgg
    71581 atgcttgaac aaaagcagaa tcctcgttac ctgtttgaga caagctggct gatcaatacg
    71641 atcaacaata ttgccggttt cggcgggttt gttagtgtgg ggcttcgctc tgaactatat
    71701 ggacaaaaga aagagagcag aaacgtgatc caagcattat cgaaagtttt cttgtttttg
    71761 atggctggac tttctattta cagtctgctt tcttttttac ttgtagtctt cacgccagtt
    71821 gctccttttt tgaagcaata ttggatatgg ttgatcggcg gcggtctata ttttcctgtc
    71881 gtctttttat ttactacttt caaaaagaaa ggattccttg gtggtacctc acttaaaact
    71941 caaggacagc tattgctagt ttcattgttg gaatggaccg gtgtcctgtt tagtttcctt
    72001 tctgttggtt atttaatgga aatccggatt gatctgtggc aaacaattcc attattcatt
    72061 gctgcttcag tgataggaat cgtgtctatg attccaggag aaattgggag tttcgatgta
    72121 atgatgatta tcgggttgtc cgctattggt gtgccaagag aaatagtcgt tgtgtggatc
    72181 ttgctctatc gcctgttcta ctacatcatt ccgtttttga ttggaatcgt ctttttcttc
    72241 aaaaatatcg gttccacttt tgatcagcgt tattctggta tccctaaaca attagcaaca
    72301 gaaattgcgc ataaaatcgt ggttgtctta ttatactttt ctggaatcat gctagtactt
    72361 tctgccacta tcccgcaagc ttttacagag tttcgctggc ttcatagttt gaacccattg
    72421 aaatttcatt ttattatcca atttcctagt atactattgg gatttctttt aatcattatg
    72481 ggcagaggga tcgcagcacg tgtgaaacga gcatatttgc ctacaatctt cttgattgtt
    72541 ctagctttac tttatgtttt attaagtgat tttagtttca caccggtggt tttcttgtct
    72601 attctgcttc tgatcattct tgcttctaaa aacgaattat ttcgggaaca attgatctat
    72661 tcatgggagt ggcgtacgat cgatggaatc attattggtg cactgtcctt gctttacatc
    72721 gttattgggg tatacaatct gccggatttc cctcatcggc gtcaccattt tatttccttt
    72781 ttcttatttc cttctgaaaa gatttggttt tcgggattgt tggccatcat cgctgttagt
    72841 ttcatgatcg ttctatttgt tcatttcctt caaggagaaa agaaacagat tggcgaagcc
    72901 tttaatgaag aaaaggccct gaagatcctt acaacttatg gaggaaactc cgatagtcaa
    72961 ttgatcttct taaaagataa gcggatgttt gcctatgaaa aagacggtga accaactgtt
    73021 cttctccaat tcgcttgctt caataataaa tgtattgtaa tgggtgatcc ttctggtaag
    73081 aaagaagatt ttccagaagc aatcgaagct tttatcgaag aaacagatcg actatgctat
    73141 ttaccagttt tctacgaaac aagcgaagag attgttatga ttttgcatga attcggctac
    73201 gatttcatta agatggggga agaagcttat gtagacctga atagttttac aacttcaggg
    73261 aaaaaaatga agggcactcg tgctgtcttg aatcggatcg aacgagaagg atttacattt
    73321 gatgtcttac aaccaccatt ttctactgag caaatgagta ctttcaaaaa tatctctgat
    73381 aattggttgg gttcacgaaa ggaaaaaggg ttttctttag gctttttctc tgaagattat
    73441 ctacaaagag cgcctattgc agttgtcaaa aatacccaag aagaaattgt cgcttttgca
    73501 acgatcatgc ctacttacac gaacaataaa gtaggaacga ttgatctgat gcgatatgat
    73561 tctaaaaaag caccttctgg cagtatggat ttcttgttct tgcatttgtt tgcctatata
    73621 aaagaagaac atatccaatg gttcaattta gggatggccc ctctttcaaa tgtcggaact
    73681 tccagaaaga gttttttaca ggaaaggatt gcatacttgg tttacgagtt tggttctcat
    73741 ttttattctt tccatggatt aagagaatat aaaagcaagt acgctacaaa ctgggtctca
    73801 cgttatactc tatattctag agatagttgg attgcatatg ttatgattgc tttgctgatt
    73861 attgataatg cgccagttga gcagacacaa ggtagaataa gtggtttgaa aaaatggata
    73921 cgaagaaagt attaaaaact gcataccgat agatgaacac tctcatgatg aataaaagag
    73981 actgaagtaa aacgaaagtt catgcttcta caatctcttc ttatacaatt gaagaacaaa
    74041 gagcggtcca aacagaaatc ctgtttggac cgctctttta ttttaaacat ttttcattac
    74101 caaaatattc gagtcgcgtt tcttatgctt cgatctctgt aacgatacct gaacctactg
    74161 tccgtccgcc ttcacgaatt gagaatgtcg ttccgttttc aacagcgatt ggatggatca
    74221 agtcaacatc aatcgtcacg ttgtcaccag gcatgaccat ttctgtatct tctggcaatg
    74281 tgattgttcc tgtaacatca gttgtgcgga agtagaactg tggacgatag ttgttgaaga
    74341 atggagtatg acgtccacct tcttcttttg tcaacacgta cacttctgct ttgaattttg
    74401 tatgtggagt aatggaacct ggtttagcaa gtacttgtcc acgttcgata tcgtcacgtt
    74461 ggatcccccg taacaacacg cctacgttat ccccagcttc cccgtaatct aacgttttac
    74521 ggaacatttc tacacctgtt acaactgctt tttgtgtttc aggtttgatc cctacgatct
    74581 ctacctcatc accgacacga acagcaccac gatcaatacg acctgaggca acagttcctc
    74641 gacctgtaat tgagaagacg tcttccactg gcaatagtaa tggtttatct gtatcacgtt
    74701 ctggtgttgg gatatattca tctactgtat ccatcaattc cataatagca gcttcagcat
    74761 ctggatcgcc ttgcaatgct tttaatgcgg aacctttgat cacaggagtg tcatcgcctg
    74821 ggaaaccata ttcgctcaat aactcgcgaa cttccatttc taccaaatcg atcaattctt
    74881 catcatcgac taaatcaact ttgttcaaga aaacaatcag gtattttaca ccaacttggc
    74941 gtgacaataa aatgtgttca cgtgtttgag gcatcggacc gtcagttgca gaaacaacta
    75001 agattgcacc atccatttgt gctgcccctg taatcatgtt tttcacatag tccgcgtgtc
    75061 ctggtgcatc aatatgcgcg tagtggcgtt tttctgtttc atattctacg tgtgcggtat
    75121 tgatcgtaat cccacgttca cgttcttctg gagctgcatc aatactagca tagtcttgag
    75181 gatttgctag gccttttttg cctaatactg tagtgatcgc agcagttaaa gtagttttcc
    75241 catggtcaac gtgtccaatc gtaccaatgt taacgtgtgg tttacttctg tcataatgtt
    75301 cttttgccat aatttacaaa cctcctaaat aatatagatc actgaaaaat ttcagctctc
    75361 acctttttat tatagtcttt ttttgactaa atcaaaaaga atacgtccga cttacttttt
    75421 gtaagattcg aactaagctt attatacgtc aatattggtc aaagtcaact aagagcactc
    75481 gtctgtctta tacgcgaaaa aagtaccttt tataaataaa aaaatgtgtt ttttgagaaa
    75541 aacttcaaga tagtccttaa ctttcttttg aagcaataag aacaaaatga ggaaaagttt
    75601 aaaattcagc tgttgccaac aactttcaat agacgaaatc ttctatttcg ttaataataa
    75661 ggaaagaggt gaagtacatg aaaaaattaa ttggcgtact tgtcgtttta ggcatatttc
    75721 tcggcggaag ttttgcagct tatcattatt tttacggcgg cgaagcttat tatacaaaaa
    75781 tcacgacgaa tggcgaaatg tcagctaccc aagcagataa cggagagaaa ttcacgactt
    75841 atacctacaa gcaagcggct tataataaag atggtgaaaa aaaacaggtg acgcttcgcg
    75901 aagaaagaga acatccatta aagatgaatg cgtatcttaa actcaaagtc aatcctcgta
    75961 aaggtgtatt aagctgggaa gaaataaagg cttctgaagt gcctaaaaat gcggcagaaa
    76021 aaatcgataa ttagtaaatg gtcttaaaat tttattagat aaaa
//
LOCUS       DAZKOB010000005        62743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000005 DAZKOB010000000
VERSION     DAZKOB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.005"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..976)
                     /locus_tag="ACG8M6_000326"
     CDS             complement(<1..976)
                     /locus_tag="ACG8M6_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0456025.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
     gene            complement(1231..1740)
                     /gene="mreD"
                     /locus_tag="ACG8M6_000327"
     CDS             complement(1231..1740)
                     /gene="mreD"
                     /locus_tag="ACG8M6_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0456026.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1744..2598)
                     /gene="mreC"
                     /locus_tag="ACG8M6_000328"
     CDS             complement(1744..2598)
                     /gene="mreC"
                     /locus_tag="ACG8M6_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0456027.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2753..3391)
                     /locus_tag="ACG8M6_000329"
     CDS             complement(2753..3391)
                     /locus_tag="ACG8M6_000329"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0456028.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3392..4042)
                     /gene="rpe"
                     /locus_tag="ACG8M6_000330"
     CDS             complement(3392..4042)
                     /gene="rpe"
                     /locus_tag="ACG8M6_000330"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0456029.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4055..4954)
                     /gene="rsgA"
                     /locus_tag="ACG8M6_000331"
     CDS             complement(4055..4954)
                     /gene="rsgA"
                     /locus_tag="ACG8M6_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0456030.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5119..7188)
                     /gene="pknB"
                     /locus_tag="ACG8M6_000332"
     CDS             complement(5119..7188)
                     /gene="pknB"
                     /locus_tag="ACG8M6_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0456031.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7185..7925)
                     /locus_tag="ACG8M6_000333"
     CDS             complement(7185..7925)
                     /locus_tag="ACG8M6_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0456032.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7938..9296)
                     /gene="rsmB"
                     /locus_tag="ACG8M6_000334"
     CDS             complement(7938..9296)
                     /gene="rsmB"
                     /locus_tag="ACG8M6_000334"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0456033.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9296..10234)
                     /gene="fmt"
                     /locus_tag="ACG8M6_000335"
     CDS             complement(9296..10234)
                     /gene="fmt"
                     /locus_tag="ACG8M6_000335"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0456034.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10227..10718)
                     /gene="def"
                     /locus_tag="ACG8M6_000336"
     CDS             complement(10227..10718)
                     /gene="def"
                     /locus_tag="ACG8M6_000336"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0456035.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10733..13141)
                     /gene="priA"
                     /locus_tag="ACG8M6_000337"
     CDS             complement(10733..13141)
                     /gene="priA"
                     /locus_tag="ACG8M6_000337"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0456036.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13394..13699)
                     /gene="rpoZ"
                     /locus_tag="ACG8M6_000338"
     CDS             complement(13394..13699)
                     /gene="rpoZ"
                     /locus_tag="ACG8M6_000338"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0456037.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13704..14318)
                     /gene="gmk"
                     /locus_tag="ACG8M6_000339"
     CDS             complement(13704..14318)
                     /gene="gmk"
                     /locus_tag="ACG8M6_000339"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0456038.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14805..15089)
                     /locus_tag="ACG8M6_000340"
     CDS             complement(14805..15089)
                     /locus_tag="ACG8M6_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456039.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15107..15997)
                     /locus_tag="ACG8M6_000341"
     CDS             complement(15107..15997)
                     /locus_tag="ACG8M6_000341"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0456040.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16017..16235)
                     /locus_tag="ACG8M6_000342"
     CDS             complement(16017..16235)
                     /locus_tag="ACG8M6_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0456041.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16205..17905)
                     /locus_tag="ACG8M6_000343"
     CDS             complement(16205..17905)
                     /locus_tag="ACG8M6_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0456042.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18172..18945
                     /locus_tag="ACG8M6_000344"
     CDS             18172..18945
                     /locus_tag="ACG8M6_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456043.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19026..19595)
                     /gene="thiT"
                     /locus_tag="ACG8M6_000345"
     CDS             complement(19026..19595)
                     /gene="thiT"
                     /locus_tag="ACG8M6_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0456044.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19661..19763)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19769..20740)
                     /locus_tag="ACG8M6_000346"
     CDS             complement(19769..20740)
                     /locus_tag="ACG8M6_000346"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0456045.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20834..22207)
                     /gene="glmU"
                     /locus_tag="ACG8M6_000347"
     CDS             complement(20834..22207)
                     /gene="glmU"
                     /locus_tag="ACG8M6_000347"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0456046.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22710..23528)
                     /gene="purR"
                     /locus_tag="ACG8M6_000348"
     CDS             complement(22710..23528)
                     /gene="purR"
                     /locus_tag="ACG8M6_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0456047.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23695..24519)
                     /locus_tag="ACG8M6_000349"
     CDS             complement(23695..24519)
                     /locus_tag="ACG8M6_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0456048.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24492..25172)
                     /locus_tag="ACG8M6_000350"
     CDS             complement(24492..25172)
                     /locus_tag="ACG8M6_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456049.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25195..26133)
                     /locus_tag="ACG8M6_000351"
     CDS             complement(25195..26133)
                     /locus_tag="ACG8M6_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0456050.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26378..27229)
                     /gene="ispE"
                     /locus_tag="ACG8M6_000352"
     CDS             complement(26378..27229)
                     /gene="ispE"
                     /locus_tag="ACG8M6_000352"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0456051.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27383..29956)
                     /gene="mprF"
                     /locus_tag="ACG8M6_000353"
     CDS             complement(27383..29956)
                     /gene="mprF"
                     /locus_tag="ACG8M6_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0456052.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30097..30903
                     /gene="proC"
                     /locus_tag="ACG8M6_000354"
     CDS             30097..30903
                     /gene="proC"
                     /locus_tag="ACG8M6_000354"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0456053.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(30982..32538)
                     /gene="rny"
                     /locus_tag="ACG8M6_000355"
     CDS             complement(30982..32538)
                     /gene="rny"
                     /locus_tag="ACG8M6_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0456054.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32949..33998)
                     /gene="recA"
                     /locus_tag="ACG8M6_000356"
     CDS             complement(32949..33998)
                     /gene="recA"
                     /locus_tag="ACG8M6_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0456055.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34107..35348)
                     /locus_tag="ACG8M6_000357"
     CDS             complement(34107..35348)
                     /locus_tag="ACG8M6_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0456056.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35588..36166)
                     /gene="pgsA"
                     /locus_tag="ACG8M6_000358"
     CDS             complement(35588..36166)
                     /gene="pgsA"
                     /locus_tag="ACG8M6_000358"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0456057.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36375..37298)
                     /locus_tag="ACG8M6_000359"
     CDS             complement(36375..37298)
                     /locus_tag="ACG8M6_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0456058.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38037..39323)
                     /locus_tag="ACG8M6_000360"
     CDS             complement(38037..39323)
                     /locus_tag="ACG8M6_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0456059.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39316..40584)
                     /locus_tag="ACG8M6_000361"
     CDS             complement(39316..40584)
                     /locus_tag="ACG8M6_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0456060.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40836..41297
                     /gene="mscL"
                     /locus_tag="ACG8M6_000362"
     CDS             40836..41297
                     /gene="mscL"
                     /locus_tag="ACG8M6_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0456061.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41356..41802)
                     /locus_tag="ACG8M6_000363"
     CDS             complement(41356..41802)
                     /locus_tag="ACG8M6_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0456062.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41895..42140)
                     /locus_tag="ACG8M6_000364"
     CDS             complement(41895..42140)
                     /locus_tag="ACG8M6_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0456063.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42255..42833)
                     /locus_tag="ACG8M6_000365"
     CDS             complement(42255..42833)
                     /locus_tag="ACG8M6_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0456064.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42910..43440)
                     /locus_tag="ACG8M6_000366"
     CDS             complement(42910..43440)
                     /locus_tag="ACG8M6_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0456065.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43444..44304)
                     /gene="rlmB"
                     /locus_tag="ACG8M6_000367"
     CDS             complement(43444..44304)
                     /gene="rlmB"
                     /locus_tag="ACG8M6_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0456066.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44297..44692)
                     /locus_tag="ACG8M6_000368"
     CDS             complement(44297..44692)
                     /locus_tag="ACG8M6_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0456067.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(44689..46101)
                     /gene="cysS"
                     /locus_tag="ACG8M6_000369"
     CDS             complement(44689..46101)
                     /gene="cysS"
                     /locus_tag="ACG8M6_000369"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0456068.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46098..46634)
                     /gene="epsC"
                     /locus_tag="ACG8M6_000370"
     CDS             complement(46098..46634)
                     /gene="epsC"
                     /locus_tag="ACG8M6_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0456069.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(46720..46959)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(46956..48416)
                     /gene="gltX"
                     /locus_tag="ACG8M6_000371"
     CDS             complement(46956..48416)
                     /gene="gltX"
                     /locus_tag="ACG8M6_000371"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0456070.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(48586..49743)
                     /locus_tag="ACG8M6_000372"
     CDS             complement(48586..49743)
                     /locus_tag="ACG8M6_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0456071.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(49777..51147)
                     /gene="radA"
                     /locus_tag="ACG8M6_000373"
     CDS             complement(49777..51147)
                     /gene="radA"
                     /locus_tag="ACG8M6_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0456072.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51210..51737)
                     /locus_tag="ACG8M6_000374"
     CDS             complement(51210..51737)
                     /locus_tag="ACG8M6_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0456073.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            51967..52773
                     /gene="proB"
                     /locus_tag="ACG8M6_000375"
     CDS             51967..52773
                     /gene="proB"
                     /locus_tag="ACG8M6_000375"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0456074.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            52770..54017
                     /locus_tag="ACG8M6_000376"
     CDS             52770..54017
                     /locus_tag="ACG8M6_000376"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0456075.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54093..54437
                     /locus_tag="ACG8M6_000377"
     CDS             54093..54437
                     /locus_tag="ACG8M6_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456076.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(54554..54880)
                     /locus_tag="ACG8M6_000378"
     CDS             complement(54554..54880)
                     /locus_tag="ACG8M6_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456077.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(55495..56697)
                     /locus_tag="ACG8M6_000379"
     CDS             complement(55495..56697)
                     /locus_tag="ACG8M6_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0456078.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            56814..57329
                     /locus_tag="ACG8M6_000380"
     CDS             56814..57329
                     /locus_tag="ACG8M6_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0456079.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(57528..57974)
                     /locus_tag="ACG8M6_000381"
     CDS             complement(57528..57974)
                     /locus_tag="ACG8M6_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0456080.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(58021..59499)
                     /gene="msr(C)"
                     /locus_tag="ACG8M6_000382"
     CDS             complement(58021..59499)
                     /gene="msr(C)"
                     /locus_tag="ACG8M6_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0456081.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(59853..60482)
                     /locus_tag="ACG8M6_000383"
     CDS             complement(59853..60482)
                     /locus_tag="ACG8M6_000383"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0456082.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(60466..60930)
                     /locus_tag="ACG8M6_000384"
     CDS             complement(60466..60930)
                     /locus_tag="ACG8M6_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0456083.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(61417..62685)
                     /locus_tag="ACG8M6_000385"
     CDS             complement(61417..62685)
                     /locus_tag="ACG8M6_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0456084.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
BASE COUNT        17440 a        13521 c        11203 g        20579 t
ORIGIN      
        1 ctgttgttga ttcttctgtt gctgaagatt caggcgctgt tgattcttct gttgaagatt
       61 caggtgttgt tgattcttct gttgctgaag attgtgtagc tgatgatgat tcttcagtca
      121 ttgaagattg tgtagctgat gattcttctg ttgctgagct ttgtgcagta gatgaagctt
      181 gagtagcttg tgcttcagcc tctgcttgtt cgcgtgcttc tttttcagct ttttcttgtg
      241 cagcttgttg acgagcttgt tctgctagac gttgttgttc acgaatacga gcttgctctg
      301 cttcagcttc tgctttttga cggttcaagt cagctttctt gtcttcagca gttgcttgtt
      361 cagcagctaa tgaagttttc agtacgttca aatcagcttg tttagataaa agatcgcctt
      421 tttgagattc caaagcagct tggttttcag ctaattcttt tagtttagca tcgttttcag
      481 cttttttgtc ttctacagct tttttatctt gtttttgttg ttccatcaag tcgttgtttg
      541 cttttaccat cgttgtcatt gcttgaacgc gtccaatagc atctgctaat gaatcagcat
      601 tcaatacagc atcgatgtag tttgagcttg tgttgcttac ttgtgcttcg cgtgcttgtt
      661 tttggattgt atcttcacgt ttttcaatac gttcttgtaa atctgcaatg tcttttacta
      721 attgtgcaga ttcttgacgc aatgtatctt gttttgctaa taggtcttgt gcttgtgtgt
      781 tgatttcaga aacttggctt tctaaggcat cgatttgtga ttgtgcatct gcttgttgat
      841 ttttcaagtc tgcaatcttt tgatcttgct gttgtatctg agaatcaaaa tcgtccgccg
      901 cggcagcgat cggtgatgct acggcagtca aggtcattga acataccatt actgctgata
      961 ttaaactctt tttcactctt cattcctccg actggcttaa tttaataaat atctaaatat
     1021 atttaagtct ttcttaacga tataaaaatt gtaccatagc agtgtgtcaa cgatataata
     1081 gttatgttac aaaaatgttt caaaacaact tttaaaaatg acaaaactgt tcgtttcaga
     1141 aaatgatcat gaaaaagccg cgttaagtct agcatttctt aagataacaa cgattctttt
     1201 tcgctttcga cctaaaaata aaaaaagaaa ttattcatca ctgaataatt tcttaaaggg
     1261 aaaaatgaat aatacaaata taatcatgtt caacagcagt gtaggtccta aaaaccttgt
     1321 aataaaatac gtgttattta ctactgctaa tttaaaaatc aactggacca ccatcgtaaa
     1381 caattcataa cccgtgacaa agatgatcat actgaaaaat tcagtaaaga tgttgacatg
     1441 gataacgtct ttcattacat acatcaacca tacaattaaa gggagagcta ctgcatagat
     1501 accgataacg ccaatatagt aagcgtcata gatagccccg agaacaatcg tggtaatcag
     1561 taaataacgt ttttcaaacg ccatactgca acataataat gccaatatca gaaagtgggc
     1621 atttgacata taggtgcctt tcgaccactc ccctagcatt cttgttaaat gaccatctat
     1681 caacatcaaa aagaacaaaa cgattggcag atagtacttc attgtttctt tcttcaacat
     1741 ggactaatcc tccgctagcc gttggacaat cgtcacaact gacacgtcat acatttctgc
     1801 ataaggtttc acataaactt cccgatccag cccataacta tctggtttcg ttttaataac
     1861 cgtaccgatc ggcaagttag ctggagaatt ccctccaaga ccggatgtct ggacaacatc
     1921 cccttctttg atatccgtat caccagttaa ttgggtcacc actaaggcat ggagcttttc
     1981 atcatagttt ttaagcaaac caaacgcttc gccattagcc gaagataccc gtactggaaa
     2041 atgattggag ctttcattag aggatgacag taattcgatt ttagacgaag ccgtattgac
     2101 ctcgatcact cggccaatca atcctttttg cgacatgact gccatatttg cttcgatacc
     2161 gtctttagaa cccttgtcta cgatcagcat gtcctgccaa ctgtcaggcg aacgagtaat
     2221 gacatttgcc gttacttttt cgtaactagt cagcgtctga ttcaactcaa gttctttttt
     2281 caacgcttcg atttctctct cgtaattctt gttttgctgt gaaacgtcat catagctatc
     2341 gattttttct tttagccgct cattttcggc ataagttgaa aaaagattat ggacagaatc
     2401 tactccattt cgtacccacc gtactggggc agagattgtt ttatcaacca ttgcaacact
     2461 gtcattaacg atggattgga agaaattggg ctttccttca ttcgctcgct gcgcagcggt
     2521 cacacttagt atcgtcacca cgatgataac aataaccaag gtaataataa tatttttatt
     2581 aggattgaac ttcttcacaa agacacctcg aattaattag actcttgtgt tcaattttat
     2641 cactaaatgc cgtgaaacaa aaggtaagtt gctcctctta agcttttttt tagcaaataa
     2701 tagattctgt catttttagc ctgaaagaca aattttttta tttgtttatg gatcaatctt
     2761 tgctttgaat aactgcaatc ataccatctg aaaaagaaaa ttctgcggta tctgaaagaa
     2821 attcgttact tgcataagat gtcggatatt ctacttcttt attttccaat acatacttgc
     2881 tgccttttaa tgtaagattc gagacgggag tcaagcagca ataagctaaa tacttcatat
     2941 ctggtatctg tcttactata tgctctccag gcaaataata agacaaataa ttttgacaat
     3001 ctctgataat aagctgatgg gcaaagggtt tgaagcgagg ttccatacct aaccagagat
     3061 tggctaaaag atgatccaat cttccgcccg tagctccaat caaagtgact tccgcttgcg
     3121 ggtaacgttc aaatgttttt aagataccta actgtgtatc tgtatcgtct ttttctgcag
     3181 gggaacgggt atactctttg gcttttgcaa atacttcctt ctgctcttcg ttcgacaatg
     3241 aatcaaaatc acccactgcc agatctactg ggtagcctgc ttgaagcagg tacagcgcac
     3301 cacggtctat cccgacatag gcatcgtatt tggacagcag agactgcggc cagttttgcg
     3361 gattacctcc agcagctaat aaaattttca tttagtctaa tgcctctttt aatcgttcga
     3421 tttgtccgac tggattttcg gctccataga tatatgatcc tgcaacgaag acatccgccc
     3481 ctgcatcttt acagattttt gctgtttctg gaacgattcc gccatctact tcgattgcat
     3541 aagtccaatt gttctctcta cgtagatcag cgagttccgt aattttttct actgtttctt
     3601 caataaagct ttgtccgccg aacccaggat tgacagtcat gactagtact tgatcagcca
     3661 aaggcaaaac atgtttgatc atggagactg gtgttcctgg attgataact actccagctt
     3721 taactcctaa gctttttgtc atttgaagtg cccgatgaat atgaggagtt gcttcgacgt
     3781 ggacagtaat gatatctgct ccagcttttg caaatgctgg aatgtagttt tctggatcaa
     3841 cgatcatcag atggacgtct aacggtaatt tagtaacagg acggattgcc tgaacaatat
     3901 ttggccctaa tgtgatattt gggacaaatc ggccatccat tacatctacg tggatgtaat
     3961 ctgcgcctcc tttttccact aactcaatgt ctctttgtaa attggcaaaa tctgcactca
     4021 aaatggatgg tgctatcttc attcttgttt cctcctattt tcgtttacct tctttttttg
     4081 catacattgg ttttcttttt tcgatttctt gtaagaattg caaataattg tcgtaacgtg
     4141 tctgtgcgat accacctgtt tctactctat gttttacttc gcagccaggt tctttagcat
     4201 gcatgcactc tctaaatttg caatggacag aagcttctac aaattcagga aattgtctcg
     4261 gcagttctcc tgcttcaatt gtcaaaaagt caatcgagct aaatcctggt gtatctgcta
     4321 ctaacccgcc atagatagga atcagttcca catgacgtgt tgtatgccgt cctcttccaa
     4381 gtgcatcaga aatctctcct gttgctaaat tcagttccgg tgatatttga ttcagtagcg
     4441 tcgattttcc tgctccagac tgacccataa agaccgatag ccgttctggg aagtaacgga
     4501 tcagttctgt cgtatcaccc gtatgattgg gtacaatcac cggatagcct attttagtat
     4561 aggtttgctt gatctcttcg attttttctg ggtcgtctga caagtcggtc tttgtcaaaa
     4621 aaataatcgg ttcgatcgac tcgtattcca aggtaactag aaagcggtca agtagattat
     4681 aagaaaatgc aggctctacg atacttgtga caatgattcc aagatcaaca tttgtgactg
     4741 gtgggcgaac taattgattt tttctcggca agatttccag aagatagccg tctgtttgat
     4801 tttcactctc aaagatcacc tgatctccga caagcggcgt gattttccga ttccggaaat
     4861 ttcctcttcc tcttgtttga taggtctctc cgtttgcgta tacatagtaa aaaccgctga
     4921 ttgcttttct gatttgtcct ttgataagtg ccacctccgt ttttgtttat tttaccatac
     4981 gaagaggaaa gtaggtgtta ttccatgcaa acttctcaaa gtatatggct tgaaaattga
     5041 tcaaaagaga tagaaacaca aaaaagagaa tgcaacaaat caatgtcgca ttcccttctg
     5101 atatatttta ttcagcactt attttgaact ttctgaggtg ctagctgatg aacttgtgga
     5161 gctggaagat tcttcttttg aagagttgct tgtgctactg gagctacttg agctgctaga
     5221 actgtctgaa gagtcttttt cagaactaga agacgctgga tctggtcctt tagagtaaat
     5281 tacgttcaca accattcctt tggtaattgt cgttcctgcc cctggtactg tacgaatcac
     5341 tttatcttta tccaccgtgt ttgaatattc ggaagtctca tgcccgatat attctgcacc
     5401 gacactagct aaataacttt gtgcttcgct ttttgtatag ccggagatat cattcaattt
     5461 aggctctgtt ccttggctta cgacaaaatt gatgtaagtt tcttctgcta caacttcacc
     5521 cgaggctgga tcctgactga tgatcgaacc tgatggtaca gtgtcgctat agtcataagt
     5581 agcactaata ttattgatat tgaatccttg tgtaacaagc caattacgaa cagtttcgta
     5641 gttttctcct acatgactac tcaaagtcac tgtttctggt cctttgctga ctgtgaaagt
     5701 aatcgtgtct gttttaggat cgacttctgt atcttctgat ggagattgcg aaatgatcat
     5761 tccgctttct acctctgaac tgaattcctc ttctttttga atcaagtcag cagaaaaccc
     5821 taattctttc aaacttgctt cggcatcttc ataactttta ccactgtaat catctaattt
     5881 gatttttttc gtgccgctac taatatacag agtgacttcg cgtttttgct tgactgtcgt
     5941 tcccgctgcc gggtctgtct tcactacatt accttcttcg attttgtcat ctgcaatttc
     6001 ttctgttttt gttgctactt ctaatttcgc ttttgcgagt gtttctctag cagaagcttc
     6061 agaaagatca gaaacatcag gaacaacaac ttctcctcgc ccgccagaag caaagaatac
     6121 agctgttccg attcctaaaa cagcaagaat agctaatagg atcagccacc attttttctg
     6181 tcctttcttt ttaggatcag gcagaacttc ttcctcctct ggtaaggttt cgttttctgg
     6241 cttgtctttt ggcagaggca tagatttgaa tgaatcaggc attggtgcat cttccgtaag
     6301 gggtgtgata acttttgttt cattatccat cacatgaggc tgccattttt cttcgtttgc
     6361 acgagctggt gataatactg tagaaagatc atcagacatt tcctcggctg acttataacg
     6421 gtctgcaggt tctttagccg ttgcccgtaa aatcacgttt tccagtgctt gtggcacatt
     6481 tggatcaagt gcttttatag atggaagatc atcttgaaaa tgttttaaag caattgttac
     6541 agcagattca ccatcaaacg gtacgctacc cgtcaacatc tcataaagga tgatccctaa
     6601 agcataaata tctgactgct tcgttgccat acttcctctg gcctgctcag gggacagata
     6661 atgaacagat cctagcatcg tattcgtctg cgtgatcgat gtttcagata aagcgatagc
     6721 aatcccgaaa tccgtgattt ttacaacacc ttcattatcg atcagtacgt tttggggttt
     6781 caaatctcgg tggatgattt gatggttatg agctaaagaa atagcagaaa ggatctgctg
     6841 cataatattt actgctgttt cataaggaac aggataatgt gtttggatat atcgtttcaa
     6901 gtccattcct ttgacgtatt ccatcaccag atactgcatg ttgtcttctt caccaacatc
     6961 gtaaacacta acaatgtttg gatgaacgag ttctgttgct gctaaagctt ctctctgaaa
     7021 tcgtcgaatc gcagtctggt cattttgaaa gtcaaatcgt aagactttaa ctgctacatc
     7081 acgatccaag attaaatcgt gcgccaaaaa tacatttgcc ataccaccgc taccgatatt
     7141 tccagtgatt tgataacggc catttaattt tttgcccaac tcgatcatac actgtcctcc
     7201 ttatattcga taagaagaac ggtgatatta tccgctccgc ctgcttcatt ggcacgatca
     7261 atcagctcag ttactttatg agacaatggg ccttcttgat tgatgatcgt tcctatttct
     7321 tcttcagaca gcatgttcgt caagccatct gaacataaca gcagtcgatc ttttagttgc
     7381 caaagatgag tagaaacatc tacttcgact gttccaggca tgccgacaga acgtgttaaa
     7441 atatttttcc ttggatggtt cactgccatt tcttcgctga tttctcctga tttgactagt
     7501 tcattgacta aagaatgatc ctccgtcaat tgcatgattt tatcttcacg aatcaaataa
     7561 gctctgctat ccccaacatt ggcaatcgtg aaccagtcat ctgacagagc ggccgctacg
     7621 attgttgttc ccataccgtt atattccggc tgttcttgtc cacgctgata aatcagcata
     7681 ttttcttctt gaatcgattg aatgaaccat tgggcgattt tttcatcgtc aacagcttct
     7741 tgtttttccc aagcttcgcc tagattcgtt accgccatct gactggcaat gtctccggca
     7801 cgatgaccac ccatcccgtc tgctaataca gcaagcttga gacctgaacg gctggtaaat
     7861 acactggcat agtcttgatt tgtgttacgt cgtcttccta catctgattg atattcaatc
     7921 tgcatttttt cacctcatta ctttttgcgc aaacaacaaa tgaaaaatcc atctgtgtaa
     7981 aactgatgcg gatacaaagt caacattttc tcttcgattg atgattgtag cataggattg
     8041 gtcaaaacat caatcctctc aaaatcaggg tgtctttcca agaaagcaga aacaacgtct
     8101 tggttttctt cttttaagat cgtacaagtg ctataagtca gaataccgga agactttaaa
     8161 gttggtgcgc agctttctaa aatttccaat tggatttttg gtaaattcgc taattcatcc
     8221 ggctgttttt tataacgaat atctggtttt cgccgaagca gcccaagacc tgaacaaggc
     8281 gcgtctacta aaatccgatc aaactgctct gcttcgaact gtttactgac ttctctagca
     8341 tccatctttt gagcgtagac agcagaagag acacctagac gtgctgcatt atcttcaatg
     8401 agtttgactt tgtgttcgtg tacatctaaa gcgaccactt ctccaccgca tgacggatct
     8461 aaaaaagaag caatatgcgt cgtcttacct ccgggtgctg cacaggcatc taagacatga
     8521 tgttcaggct cgatttgcat agacggcgct acaagcatcg aactttcatc ttggatcgtc
     8581 atgacgccat cgtggaacaa tgagctagaa gctaaatgcc cttttttcgc tactataccg
     8641 tatggactga cttggctttt ttcagctgtg atctcttctt cttgcaaaac agtgatggcc
     8701 tgatctctgg aaagtcggcg tgtatctact cgggcactag catggctagg ctgatatagc
     8761 gataaaccga gcttttcagt ttcttcgtat ccgatttgat ccaccagtcg tttcgtcatc
     8821 catagaggca ggctgatttt gacagaaagt ctttcgatag gatcagcaat gccttgcaga
     8881 tctggtgctc cttgacgctg ataattcctc aagacaccat ttacaaattt ccctgtccca
     8941 ggatttcctt ttacttttgc gatttcgacc gcctcgttta aaatagcatg tgcagggact
     9001 ttatctaaaa attctaattg gtatacagaa agataaagaa gcatcttgac ccagtcatcc
     9061 actttttttg ctttagcgat caatggtgca acgtaaaatt ccaatagtcg ctgatggcta
     9121 atcgtaccat agatcaattc tgtaaataaa cccacatctt tttgatttag ctcgctttgt
     9181 ttgatcatct cattcaacaa aagattcgag taggcgccac ccttgttcac tcgctctaat
     9241 gtcagtaatg ccatatatcg tactgatttt ttcatttttt tagggatttt tttcattatt
     9301 tcacctgctg accaatctga acttgctgac cgcttccgtt aaggaaatca tgtacggcct
     9361 gttttccttt accagctggc tgtaattcat tgatggctaa gacggtctgt ttaccgcaag
     9421 caatccatag attttttttg tctttccgga tgatcgtacc aggtgctttg tctgtagttt
     9481 ctgccaatac ttcaaccgat aataatttcc agcgtatgcc ctcataggtc gtgaatgcaa
     9541 ttggccaagg acgcattccg cgtacttggt tgtcaatttc ttctgcagtt ttatgccaat
     9601 cgattgcttc ttgttcccgt gtgatgtttg gcgaaaaagt tgcttctgac tcgtcttgag
     9661 gtactggttt ttgtccatcc aaaatattgg gcaatgtttc tagcaatagc tttcttccta
     9721 aaaggctaag cttttcaaac atcgtcccta catcatcttg tttagtgatt ggtatgcttt
     9781 cttgcgcata gattccgcct gcatccattt ttttgatcat ttccatgatc gttacaccag
     9841 tttctttctc cccattcatg atggcataat gaacaggcgc acctccccga tatttaggaa
     9901 gtaaagaagc atggacattg attgccccta attttggtac ttgcaataac ttttcaggca
     9961 agaattggcc aaatgccgca gtgacgatca catcaggcac caattcaatg atttcttcca
    10021 tttctggcga accggagatt ttctctggct gcagcacacg gataccgtgc ttcagagccg
    10081 ctttttttac cggtgtcggc gtaattactc gtttccgccc aactggacgg tcgggctgtg
    10141 ttaccactgc ctggatctcg taacctgatt ctatcagccc ttcaagaatc ggcacagaaa
    10201 aagcaggtgt gcccataaat actagcttag tcatcaagat gttcctccat atattcttca
    10261 agttcttctt ccgggatctg ctgtatcatt ttctcaatga acaagatacc gtctaagtga
    10321 tcgatttcat gctggaaggc acgggctaaa tagccaaaag ccgtcacttc tatctcttct
    10381 ccgtctcgat cataataacg cacggtcact tcatctgctc gcttgaccgt tccatagaca
    10441 tgcggaatac tgagacaacc ttctacgtca aggctttcac cttttgcttc gatgatttct
    10501 gggttaatca gttcaaattt ctcgccttca tctacttcga tcacagcaat tctcttgttt
    10561 tgtcctactt gaggagcagc gatccctatt ccgtcattcg caatcattgt ctcatataaa
    10621 ttgtctaata aagcgattgt ctcgtccgtg attacatcga ttggctgagc agttcttttt
    10681 aatttatcat tagggtggat caatatagga tagcgcataa tttggccctt tcttaaataa
    10741 agtttaatgg ttcgttgtca attgccacaa aaagaccttg tcgctgttct ttctggcttc
    10801 cattcaaaat ttcatccaaa acatgaggaa gattcggttc atgtttatat ttgatgatta
    10861 gttgataata atatcggttt ttcactctca aaatcatgct gggcgttggc cctaatagca
    10921 agctttgagg gcttaacact tcttttagct gattggcaat ctgaaagatt ttctttgccg
    10981 ctacttgttc ttcaggatgg ctggcggtta ttttcacagt aaagtaataa ggaggatagc
    11041 ctccacgatg ccgtacatgc atttcctgct gataaaactg ctcataattt tgttttttag
    11101 ccaattctat ggcgtaatgt tgaggattga atgtttggat cacgacttcg cctgcttttt
    11161 ccgctcgccc tgctcgtcct gaaacttggg taagcaattg gaatgtatgc tcactagaac
    11221 gaaaatccgg taaattcagg gaggtatcag cattcaacac cccaactaat gtcacttctg
    11281 gaaagtccaa acctttagca atcatttgtg tccctaataa tatatctgct tcttttgcac
    11341 cgaatttttg taaaatctgt tcgtgtgccc cttttcttcg agtggtatct acatccatac
    11401 gcaaaatcct agcttgcgga taaagttctc ggagttcctc ttctactttt tgagtacctg
    11461 ttccatagta gcggatttta ttgccgccac aattaggaca gcgattggga ataggttctt
    11521 catgtccgca ataatggcag cgcattgaac gtgtatccat atgcagtgta agagagatat
    11581 cgcaattagg acatggaaga acaaatccac agtccctaca catcacaaaa gacgaatatc
    11641 ctcgacgatt gagcagtagg acagtttgtt cttttttgtc taatcgattt tgtatttttt
    11701 cctgcaaatt agcagaaaaa gtagaggtcc gatgattctg aaactcttca cgcatatcta
    11761 cgatctcaat agctggaagc tgtgcagctg ctttggctcg gtggtttaac tgcaataact
    11821 gataaacgcc tttttgagct cgtgctcttg attctaaaga gggcgtcgcg cttcctagaa
    11881 ctattgggca atgatgatat ttactgcgcc aaattgctag atctctcgca tgataacgcg
    11941 gtgtctcatc ttgtttgtaa cttgattcat gttcttcatc gataatgatc agcccgatat
    12001 ttttcagcgg ggcaaaaata gcagatcgtg cgcctactac tacttgcgct tcttcccgct
    12061 caatcttgcg ccattcatca tatttttcac cttgggaaag tccgctatgt aagaccgcaa
    12121 ctgattctcc gaagcgtcct ttgaatcgct cgaccatttg cggagtcaat gcgatttcag
    12181 gcactaacat aatagccgtt ttattttctg acaaaacatc agcgattgcc tgaagatata
    12241 cttctgtttt cccgctgcca gtgattccct ccagcaaaaa gacctgatct ttttgctgct
    12301 gacctgctgt gatgatttgt tcgacagcat ttttttgttc tgcattcagt tccaatgccg
    12361 tcgtttggtc aaatactcgg tctttataag ggtcgcgata acgttctgat tctataaact
    12421 ccagccagcc cttttctttt ccttgattca agacagcagt actgattcct ttatttttga
    12481 aataagcaat tggttcttct tggctcagtt cagataaaca caaaagaagt tctttttgtt
    12541 tcttggcatt ttgccgtaat tttgcccatt cttcttcgat ttgcttttca gttaacaaag
    12601 aacggaccaa acgaatcgtt tttactttat ttcgtgtatg gacttcatat ttgatatcta
    12661 ctaattgctc ttggcgccag cgaatcaatt gagaaaggct gccttcttct tctgcttcct
    12721 tccaggaaat cacttctcgc tgatcaaaca atacctgaac ttctggtgtg tctgccaaag
    12781 gatagatcaa tttatcatat tctgctttca tcacactcgg aagcatcgtt tgtagacaag
    12841 tgattttgaa tgcgtaagta atttttttca tataatcagc taatgaaagt aattcttcat
    12901 tgaccaccgg agcaaggtct agcaaacgaa taactgcctt caaagatgga ttagctgatt
    12961 cttctgattt ttggatagcc ataacaaatc cttggacatg acgattgcca ttaccaaaag
    13021 ggacttctac tcgcatcccg acttgcaaag ctgtttccat ttcagaggga accaaataag
    13081 tgaatggctg atcggtctgc atagtaggta cgtccacaat gacttcagct gttaatgtca
    13141 tggttcgttt cctccttttt ttgtcttttc ttatcaagcg acaaaaagtt ccttgcctat
    13201 tttactagag atcgatggat ttgtcttatc ttctgccaaa tcctaacaaa aaattaacag
    13261 cagataagaa aaaaaagaac tgtgtcaaaa agttcagaca cagccttttc taccagacaa
    13321 taagtggcga atgtttgaaa aggaaacagc tgggtttgct tttcttttta gatgttcgct
    13381 ctatttttct ttattacatt ttttgctctt cacggatacg tgattccaat tcgtgttgtt
    13441 cacgctcttt ttgtgcttga cgttcctctt cttccatctt caaacgttca cgttttaatt
    13501 caggatgtgg atcattgact acatttcctg catcgatttc ttctaaagct tgaccgacgc
    13561 tttttactga atcaaaagaa tctagagtag gctgtgcact tgcatccaat tcatgcgcgc
    13621 gtttacttgc taaaatcact agagaatatt ttgaatttac acgatctaat agtgagtcaa
    13681 ttgaaggttt taacatcata agactacatc tcctttaaca ttttaatata tttaccgatt
    13741 acgcgatcta ctcgaaaatg ttcactggca ataatgtttt tgattcgttc aactgctagc
    13801 ggcacttcat catttacaac agcatagtcg taaagagcca tcatttcgat ttcttctttt
    13861 gcaactttca ttcgtttttc gattacttca tctgcatctg ttcctcggcc tacgatccgt
    13921 gattttaatt ccgctaaatc tggcggagtc aaaaagatga atacaccatc gggcactttt
    13981 tctttgactt gttttgctcc ttggacttca atctccaaga aaacatcttt tccttcatcc
    14041 aatgtcttgt tgacatatga caaaggagtt ccataataat tcccgacata ttctgcgtat
    14101 tccagcatct ctccagcttc gatcattgat tcaaactctt ctttgcttcg gaagaaataa
    14161 tcaacgcctt cgacctcccc ttcacgcatt ttacgggtag tcattgagat cgaatactga
    14221 aaatcattat cctcactctc aaaaattgct tttcgtactg tgccttttcc tacaccggat
    14281 ggaccagaca acacgattaa taatccacgc tctgacatga cgacgtcctt tcaactagtc
    14341 tttttgtact tatcgtttat tttgcacttt ttttctgcaa ctttcaaggt tatctttaat
    14401 tatagctaga aattggtaaa aaagctaccc tgtaccaaga ggtcgtaaaa aagctgtcct
    14461 gcatcaagta ataagaacag ccaaacataa acagcttctc atgtttttcg tttggttggg
    14521 gttattatcg cagatgcaag cttttgaacg ccgtgtataa gaggtcgtga agaagccgat
    14581 tagtttcgag tatgaagaaa aattttgtga aaatagtttc tcacattttt gcataaattt
    14641 ggcttaatac cgagaaaatg gtttttgaac atcacttatt caaaataaaa aaagattgtg
    14701 acacgaattt tgtcacaacc tttaaatgta ttcctagagc aaagaagcta agcgaagaaa
    14761 tccaattctg tttatttatt gtatttcttt gttcagcatc cttgctaaaa gcccatattg
    14821 gacaaagtat tcactacacc catgatctca tcttttcgag aacgtgcttt ttctgtcgga
    14881 tcttctacct cttctgtgtc aattcgaaca ataaatttcc gattcatcgt cacgtaagca
    14941 ccgtcgttga tcaaaataaa cagttcgttc tcattatagt ctgcacaggc atctttccat
    15001 acgtcaaagc cgtttttttc agaagggata tcccgtttaa ccacagtttt tccacttaat
    15061 tcgtggaacg ttacgtcata atatttcatt atctctacct ctttcgtcat tcaatatttt
    15121 gtacttgttc tctgattttt tctaagatcg tcttagactg gacgacttga tttttaattt
    15181 cgattgcatt tgatttggaa ccgatcgtgt tgatttcccg attcatttct tggatcagga
    15241 aatctaattc ccgacctaca ggacattgtg cttcgataag ttcatctagt ttttgtacat
    15301 ggatcgccag acgatccagt tcctcgtgaa tatctccgcg ttccaacaag atggctagct
    15361 ccgtcaacaa ccgttgctca tcgactttcg ctccaagaaa ttcctccagc tttttttgat
    15421 accttatttg aaattccttt tcatagattt ctacaaaact atcgagtttt tttagtactt
    15481 ctttgaattc ttcagtattc tttttcaaca ctaaagctaa agcttgacct tctttttctc
    15541 gactcgcctc aatagaatca agtgcgcttt tcaatgtatc tagagctaac tgtgccaatt
    15601 cttccatgct ctcttccggt ttttcttcta ccgttacgaa gttttcgtcc agcacgaagc
    15661 gttccgccat ccgactaatt gacacttgat tttgactacc gtatcgttgt tgaattccgt
    15721 ctgtcaattg agtgataagt gaatcaatca attcccagtt gatgacgatc tgtttttcgt
    15781 ttttgccaat ataagctaga ttcacaaata cttcgattcg accgcggctt aactgctgtt
    15841 taataagctg acgaaaatcg ttttcaacaa agttcaggat tttaggactt cgtatttgaa
    15901 catctagaaa acgctggttg acacttttta tttcaatatc taactgatac gcttttgttt
    15961 cgcggatggc tttgccaaaa ccagtcatac ttttcattcg acagggtcct tcctcattac
    16021 atattttcta ttgctttttg cagttgttca aactcttcgt tcgtcaatgt caggcctttc
    16081 cccatttttt catggtctgg cgcccaatct cgaagatcga attttggcgg gcgaccattc
    16141 cagctgacta ggttcagttc tttacgccac cccttgttgt tttctgacaa tatagcgatt
    16201 tcttctacga tttcatatga aaattcttgt gccatggttg ttcctccttg agataatata
    16261 aatataggat cagtaatgtg tcaactggtt gcttaagcaa ttcatgctgc aaacgatcct
    16321 tgcgttttcc ttgtagcaga taatcaaaat aaacagtttt cgtgcgctca ttttgtattt
    16381 tttgctggta ttttcgatac cttcgccatt cttcagtact taattgacca atttgtgcca
    16441 ataaatcatc cggcatactt tttatatctt gttggcagat catttgaatc atcatgtcta
    16501 cttgatagat ttcattgggc agtaaaaaat gtttttttgc taaaagttct agcatcgcca
    16561 atccgcatgt ctctggtacg accctgttct tatataaatt acgcagtttt tgccaaaatt
    16621 ttatgctttt gaaaaaagat aattctaact gaatttcttt tgttaagtaa gacaaaaatc
    16681 ctcgccagta tgttcttgca ggtttaacag aatgaaatgt ataccagttc ccatagtaat
    16741 agcctaatcg ttttttcatt tccagatagc ctaattgtaa atttaattgg ttcctttggc
    16801 tatcaaaaag cagaaagctg cctagtgtcc acaatgtttg acatttccaa tggataaaaa
    16861 tgtctggcat acgaattttt ttcgctgggc ctggcccttg cacatctacg acaaatgcct
    16921 ctgttgctcc tttgttaatc gcaatatcaa taggtatttt gttgcgatac ccaccgtctg
    16981 catatttatt ttttccgatc tttcgataag ccatagcggg ataaaatgaa gcagaggcga
    17041 gtatccaatc tggtatttct tctgtttttg tctgttggat atcagtcact acttccgcta
    17101 gttctggcag tttagtagac accgtatata aacgtatttt gggattatgt ttgagtaagg
    17161 acaaatccaa attgtctttg accatctttt ccaatggagc agaagaaacc ccgccttcaa
    17221 ttagtgctgt tttcgtcatt tggcgtcttt ctcgatagaa gcgttcacga aggtcttcaa
    17281 ctgcagccat ttctggtaac gcaagcactt gtcttgtcga cagctttttc cataaattca
    17341 cagctttttt catatctcct tgtaaaatca atgcgccgtt caacgcacct accgaggtac
    17401 ctgtgataat ctcaaagttg atatcatgtt ccttcagcgc ctgccacaca ccaatctgat
    17461 aagcaccgtg cgctcctcct ccacctaaaa caagtgctgt atgatagacc agctttttat
    17521 ttaattgtgt ggttccttga tagccgtttt tatgaaaaat acgcaaccat tcttctgata
    17581 gaggagattg aaataaaaaa caacagtttt ctttaataaa aaaacgagcg caagcttcca
    17641 tataccgaaa aaattttttc cattcaaaag tttcaccttc aatcaacaga ttattaaaaa
    17701 taacatctcc tctactttta gcaatagtaa aatagacggt atattttttc gatactgctt
    17761 taaaagcaca ttgctttttt gcagaacgcc aaaaagcgtc tctactttct tgatgacttt
    17821 gaaaaaatgg aaggggttgt tttgcttgct ggataggcga atacagcgac attttctgtg
    17881 aaaagtaaac tttttttatc tccatcgcgc tctccttttt cttcaagtat accagtacgt
    17941 cataaaaagg tcatgagtct cgtattcgac tttgtagatt ccataagaaa acaattcata
    18001 aaacctcctt taaataaaaa aatacaaaaa tcacaaaaat taattgaaaa tgagagtgtt
    18061 ttcaatgaaa acgtgatttt gtaatggaat tttaaaataa aagaaacaag cttttttaat
    18121 gcaaatgttg taaaataaaa gagattaaga ggaaataaga ggaggaacat gatgactgag
    18181 tcagtttatg tacatattga taccacaagt aatgctgttt taacaaaagg gctgactgct
    18241 actgactttg caaacagtat cgtgcatttt cctcaaaact tgctcttgct tgatccatct
    18301 gcttctgctg gagaatatga atcccataca gggctgaaag taatccgtgg tacagaaaat
    18361 attcagcgtt tcttttcctc ttctcgtaac aggcgtggat tcgatgaatt gaagtggatc
    18421 gactttactg atttaacgat gctgaaagaa ttgacacctt tagaaatttc agaattatta
    18481 tattttggtc atatgaaaac acatcttcat tcgccgtttt tttataaact acagaataat
    18541 tttgtatact tcgatcttaa tgatcagctg aatcggattt attatcgcta tttagatgaa
    18601 ttttatagga tttttgctag taaattaagt tcgatcttgt cagataagat caatgtaaaa
    18661 aaatcttttt tcaaaaaaga aacacctgtt gaaaaaatca gtgtggaatt attgaaagaa
    18721 atgcgtaatt tgatgcagga agggatcatt ttttcttttg atcaagttgg tttgatcgat
    18781 ggtaaatatc gtatcccaat ctatgtgatt gaagattata tttggaaatt aaaaggcacc
    18841 cggtatcgag aagaggaaat catcgctact ttaatctact ctgtcaccga tcgacactgg
    18901 caattggtag aagatgccga tctttcttat ctgccattga agtagatcta tttcttatca
    18961 aaaaataaaa aggaaacaag aaactaggac atgattctgt ctcaagtttc ttgcttcttt
    19021 tttatttaca gctcctgtcg tttatctggc acaaaaatcg aaggagtcgt ttgttggatc
    19081 cctaataaaa cgatgatcgt tacaatagct gtactcaacc cgctggcacc attcattacg
    19141 aaagaaaaga gccaaggatt catcccccat agtgcatacg ctccccagaa aaccactcct
    19201 gcaataaaat gccaaaaata tctggaaagt gcgccaatca atgcaccaaa cacgatgctt
    19261 tttctaattt gtttcgtatt atcgtctaca tagccttttt ggacatttga agccatgagc
    19321 cctgctgcgc ctgcaaaagt ataggcaatc gggtactcaa tcaacacttg aacgacactt
    19381 aaataataaa cttgtcccaa cggaaaatgt aggatgcccc aaatgaaacc tgccagcatc
    19441 cctgcctttg tccctcttct taacgcaaaa agagtcaatg gaatctgccc taatgaaata
    19501 gaaaaactgc tgccgatctg gatcggaatc aacgacaaca ccatcgctaa cgctgcaaca
    19561 atcgttcctt ctacccaaac tctatttttt cccataaaaa aacttcctcc tttgaataaa
    19621 aaagtcacaa aggaggaagc agctttcgaa aatagtcaaa cagctgttta ttcacaattc
    19681 ctacgctcgc ataaccgata caggtacgaa gggtttagaa atagattcaa tctcagccca
    19741 attggactcc cctttgtgag tttatttatt atttattttt tgtttcaaac aatggactaa
    19801 ctggtttgtt ttcatggata cgtttgattg catctcccat tagtccgcca acacttactt
    19861 catcgatttt gtcgatttta cgatcttctg gaagatagat cgagtctgta acaactagac
    19921 gctcgatagc tgaatcttct attctttgta aagcagggcc ggataatact ggatgtgtac
    19981 atgaagcata cacactagtt gcaccagctt ctttcaaagc attggcagcc aatgtgatcg
    20041 tacctgctgt atcgatcata tcatcgatca gtacacaagt tttgcctttg acttgtccaa
    20101 tgatattcat tacttctgca acatttgctt ttggacggcg tttgtctatg atcgcaatcg
    20161 gagatttcaa aaattccgct aatttacgtg cccgtgtgac accaccatgg tctggtgaaa
    20221 caacgacaac atcatcgcct ttgattcctt tttctaagaa ataattggca ataagtggcg
    20281 cacccatcaa atgatcgact ggaatatcaa agaaaccttg gatttggact gcatgaaggt
    20341 ccaaagtcag cattcttgtt gcaccagctt tttgaatcat gtttgctact aatttcgctg
    20401 tgattggttc ccgtgcacgt gctttacggt cttgacgcgc atagccataa taaggcatga
    20461 ctacgttgat tgtttttgca cttgctcgtt tcagtgcgtc aatcatgatc agcaactcca
    20521 tcaagttatc attgactggg ctgctcgttg attgaataac atagacatgc gatccacgaa
    20581 tactttcttc aatgtttact tgaatttctc catcactaaa ttgagtaaca gatgattttc
    20641 ctaactcgac gccgactgct gctgcaattt tctctgctaa tgggcgattg gaatttaacg
    20701 caaaaatttt taatcttggg tcaaagtaat gttttgacat ggggacctcc actttctttt
    20761 tcacaactct agaattctac ctaataaata gtagtcatat tttttaaata aatcaagtga
    20821 ttttgcaaaa gtcttagttt agataaggga gtttttttgc atatccttct ttattgactt
    20881 gtcttgctct cgcaattgcc agcgcatatt ctggaatatt gtctgtaatt gttgacccgg
    20941 cagcaatcga actgttttta gctacctcga caggtccgat gatattcgtg ctagaaccaa
    21001 taaagctgtg gtcgccgaca gtcgtatgat gtttgttctt tccatcatag ttgacgaaga
    21061 caacaccaca tccgacattg atttcttctc ccagcgtagc atcaccaaca taagtcaagt
    21121 gaccgacttt agttcctttg ccgatttgag catttttcac ttccacaaag tttccgatat
    21181 ggacgttttc gccaatttca gcttttggtc gtaaatgtgc ataaggtccc acatctgcat
    21241 gtttcttgac atggctgctt tcaatgactg agttttcaat gacgacgtga tcctcaatcc
    21301 tactgtctac gatttttgaa tgtgacccga tgacacaatc agaaccaatg actgtatttc
    21361 cttggatttg tacacctgct tcgatcaatg tgtctggacc gatttctacg cctgcatcga
    21421 tgtatgtcgt tgctgaatcg acaaagctta cgccattgac catatgcatc tggttaattc
    21481 gctttctcat gatctcattt gctttagcta acgcgatccg atcattgact cccattgatt
    21541 cttcaaaatc atccgtttga taagcagcaa ctgtatggcc ttcttctttt aagatttcga
    21601 tgatatctgt tagatagtat tcaccttgag cattgtttgt tccaactttg cttaacgcat
    21661 cgaacaaagc ttcgttgtca aaacagtaag ttcctgtatt gatttcttgt acaagagctt
    21721 cttctggtgt tgcatctttt tgttccacga ttttttctac gatcccaata tggtcacgga
    21781 tgattcgtcc atatcctgtt gggttttctg cttgtgccgt caagatcgtt gcacttgcat
    21841 tttttccttg gtgatattca aaaagattgt tcagcgtttc agtagtcaat aatggtgtat
    21901 cgccactgat aactaacgtt gttccttttt ttccttccaa gaaagaagca gcttgcaaaa
    21961 cagcatgtcc tgttcccaat tgttctgctt gtaacgcata tttactgcgt tctcctagtt
    22021 gtgctttaac ttgttctgct ccgtgtccaa cgatcgtaat cacttcatct ggtttcgttt
    22081 cactgacccg attaatgata tgttctacca taggtttacc agaaacggga tgtaaaactt
    22141 tatataattt tgacttcatg cgggtacctt tccctgccgc caaaataatt gcataacgtg
    22201 cgtccaactg cgtcactcct aaatatagtt tgctgatttc agtttatccc agtataccca
    22261 ttttttcaac aaaacaagtg attttctttt atcccagcgt tttctggata aaaaaataga
    22321 tagaccataa cgggcctatc tatttggtaa ccggtgctct cctgccaact cctcggtcac
    22381 aagtcacact actcataaaa catgaaaagc tgttttttga aatcgtttct tgtgacttag
    22441 agttaaacgg taggcttaca acctcttcat taaacgtttt tcaatcagct gaccttcggt
    22501 attagctcaa taaacggcaa aatatgagga gcaatttttc cgtttattga gctaatactc
    22561 aggacaggac gctgatttca caacctctag ataaacggtg ttcaaaagcc agtttctcgg
    22621 tattaagcca aatttatgaa aaaatgtgga aagccatttt cacaaaattt ttcttactac
    22681 tcgaaactaa gcggcttttt cacaacctct tattctgaaa aataatttcc tggcacaacc
    22741 gtaatatttt tcgtttgagt atctacatct ttgacataaa gcagtgacgt ataatcatcg
    22801 atggcgcgat gaccgttgaa ttttgattcg gcaaatacag tgatgccaac aagttctgat
    22861 tcgaattctt caatcaagct cttcattccg ttgactgttc cgccgccttt catgaaatca
    22921 tccactacaa ggacacgtga accacgtttc aaacttctct tagacagttc catcttttca
    22981 attctttcag aagatccaga cacatagttg acacttactg ttgatccttc tgttattttt
    23041 gagtcccggc gtacaataac gaatggcgca ttcagataat aggacacagc ttgggcaata
    23101 ggtacgcctt ttgtggctac agtcattaca gcatcgattt tttcaccaag gtatttagaa
    23161 gcaatgattc ttccaacttg acgcagcagt tcaggttctc ctaacaaatc agagagataa
    23221 acatatcctc ctggaagcag acggtcttgt tctgatagac gttctgctag agattcaata
    23281 tattttttcg cctcttcata tgaaatttca gggatgaaca atacaccacc tgcagcaccc
    23341 gggattgttt ccaaggttcc ataccctcgt tctttaaatg ttttcttgat gattcccaaa
    23401 tcttcactga tagaagattt agcagattca taacgttcgg caaagcttgt taaggaaatc
    23461 aattcatgcg ggtgattcaa taaatactgt gtcatgtcaa ctagacgttc actgcgacgt
    23521 actttcacct attccaactc cttttaagaa attttatcgt tttacattat ataagttttt
    23581 cttcaaaaat tcgagcattt tatgaaaaaa taagaaaata gttcggtatt tattaaaata
    23641 aaaagaagcc tataaaaaat ttttcgtttt tataaggctt cttttcttta taatttagtt
    23701 attttttctt ctgaaggcaa ccaatattcg tttgatgata tttacaatca ggaacaaagc
    23761 aataaagact aatgtgattg ttgctcctgg aggtgtatct agataaaacg agctagttag
    23821 tcctgacagc atccctagaa atcctacaca aacactgata gcaatcacga cattgaagct
    23881 tttgcctaat cgcataccga ttgctgcggg aagaaccatg attgcggata tcaaaagtgc
    23941 accggcaatc gggatcatca cagcaatagc cacaccagta atcacattga acatcatcga
    24001 catcaaatga acgggtagcc catccacgtg ggcggtatct tcatcaaaag tcagaacata
    24061 catcggccgt ttgaataaaa agaacaatat accgatggca acgaataaga ttcctaaaaa
    24121 gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac agataggatt ggatacttgt
    24181 tgctgaattc cctccgctca aattcatcaa tactaacgct agcgccaaac caccagccat
    24241 taaaatggca atggagattt ctgaataggt tcggtagatc gttcgcagat actccaatat
    24301 cacagctgct aatacaacga ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa
    24361 tcctaacgct acccctgcta aagagacatg agaaagcgta tcagccatca gcgattgtct
    24421 tctgatcact aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag
    24481 aaacgctctt ctcatgaatt catatgaaag caacgccatt gagaatcctc ctttctcacc
    24541 agtcggatct gtctgtccgc atactgtttg atatcttcat gatcatgggt gatcattaga
    24601 actgatttcc catggacatg cgcactatgt cggagaagac gataaaattc atttctggat
    24661 tcttcatcca tacctgttgt tggttcatca agaacaaata agtcagggtc agtagcaaaa
    24721 atacgcgcta aactgattcg ctgcttttgt ccgccagaca attcacctat tcgccgattt
    24781 cgcatatccc acatccctac agcttctaaa gcacgctgta catgttgatg atctttttca
    24841 gagagacgct tgaaccagcg gcctcttgga tatcttcctg aacggactaa ttcgatcacc
    24901 gtactaggaa aacctgcatt gaaagatgca acttgttgag gaatataacc gatacttaat
    24961 ttttttcctt cttgattttt ctcagcaatc tttatcattc cttttgaagg tttcagtaat
    25021 cctaacgatg ctttcaccaa ggttgacttg gctgctccat tttctccagt taaaatgaca
    25081 aattcacccg catctacatg ataagagaca tcttctagta caggttcatc atcatagtaa
    25141 aaggtcaaat cagatatttc gatataacgc accttacaac ctccattcta aaacttaatt
    25201 gatactttct tttaaagctt tcagattttt ttccatgaca gaaatataat tttcgcctgc
    25261 tttgatttgt tcattagtca agctttctaa aggattcaac acttctagct tcactcctgt
    25321 ttcattcgac aatgtctcag ctaccttcga agaggcattt tcttcaaaat aaatcacttt
    25381 gatgttgttt ttcttcacaa attcttttag ctcagctaag cgacttggtg tgggttcctg
    25441 atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg tattgattcg ctaaataagc
    25501 aaaagcagca tgctgagtga caaatgtgcg gtttgttgca tcttttagct catcggagta
    25561 tttttgatcc agtgcttcaa gctttttgat ataagcatcg gcattcttcg taaaagtttc
    25621 ctttttatct ggatattttt cgcctaattc ttcagcaatc gttctgactt cttttatagc
    25681 taataccgga tcgagccaaa catgtggatc gtattcatgt gaatgaccat gcccttcttc
    25741 tgattcttca tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt tagctgcttc
    25801 gatcatcaag gtctgtttgc tatccactga atcctttaac gaatccacaa aaaattccat
    25861 gtctgaacta ttatacacga aaacatctgc atcggaaatt tctgctcttt cttttgcact
    25921 tggttcgaaa tcatgcggct ccgttccagc agggatcaaa agtttcacgt ttccttcatc
    25981 ccctacgatc tcttttgtaa aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc
    26041 ctcttttttg tctgcttcct tgttcgtatt tccacatgct gcaaacagta aagcacctat
    26101 cattaccgta gctgtcaaaa gatatttttt catttatatg ctcctttcaa tgaacaaatc
    26161 ggaattttac gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag
    26221 aaatccatca atttctgatt taagcatgta tattcttgat taaatcgtaa aatggatgag
    26281 ttgctgctca tatctagcca tttcactaca ataaaaaact gaagaatacc attttaggat
    26341 tcctcagttt ttcatcacct attttacttc ttatatttta ttgcagggtt cgtactaaat
    26401 aaacttcctc acaaaaacct tttaacccat tatatactct ctgcgctctt gaataatgtt
    26461 gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct gctccgtact
    26521 tgatcatacg ctctttgatt tgatggatga ctggatgccg ctttcccgtt atatcttcta
    26581 aactgtttcc catttttttg atcatctgct gataatcttg ctctcggatg gcttcagcaa
    26641 gtccgtggat gtcttgatgt tggatggctt gcgtatctac ttctctaaac actgtacgtg
    26701 tagagacact cattcttggt ttgaccaaca caacccagca ctgtggcata gaaggtaata
    26761 cttggatttc ttcccctttg ccaccgacaa atgccgtact tccgtagaca cagtaaggaa
    26821 catctgtacc gacttccaaa ccgatttctg ccagttcttc taacgaacag cccagattcc
    26881 ataattggtt gatccctctt agagcagctg cacagtctgt acttcctccg cctaatccag
    26941 ctgctactgg aatattcttc ttaacaaaga tcttgacacc ggttttgata ttgtatttat
    27001 tttttagcaa taaagcagct tgataaacat tgttccgttc atctaccggt aaaaatgctt
    27061 tatttgtttc tacaatgatt ttgtcttctg ttatttcttc aaaaaataga tgatctgcca
    27121 agtctacgct cgacatcacc atttccaact catgataccc atcttgccgt ctatataata
    27181 catccagtcc taaatttatc ttggctggcg ctttttcaat caattccatc ctctcaccca
    27241 ttcgaaaagt ctaagtcgca agtattctat catagggaaa aataaaagta tagcttatgt
    27301 ctgcctagtg gttgattttg tcatcgtttc aacagctttc acagaatttt ctcttcataa
    27361 tccaaacaag ttctagtgat tcttataatt ctgtgagcac ttcttctgcc aaactcggaa
    27421 catttccttt ttcttttcct atcagactga gtagctgcat ggtagcaaat ccgaaattac
    27481 ttcctttcct ataagcaaaa taacgtcctt cccaatgatc gacatatttt aatttctttt
    27541 cataagcaga ctggaaggaa tagaaaaaat taccataatt atatacgata tgcatgactt
    27601 tttcttgaaa gaaagaaaaa tctgtttctc caacattagc taatggagct aagcctagat
    27661 tggcttccga gtagcccatt ttctcgtata caataaacag attggctaac agatagtcag
    27721 ctaattcctc agattcttta ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg
    27781 tcatcggctg agcggtgatg aagccaacga ctttttgatc tttacggaca ataccgacac
    27841 cgctagataa tacatatgat ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac
    27901 cttgaagcca tttttgtgaa acttcattta cagcttgatg taattcttcc ggcagttgat
    27961 cgtaccaagt aaaatggtat ccttctttca tcaaacgttg atattcaagt tgctgactca
    28021 gccgagacaa gtcttctgtt gctagatcaa caatccctgc ttcacctact ttcgtgaagt
    28081 gaaaacccaa ttcatgaagc atcactacgt agctttctga tatcttataa aacgcaagct
    28141 gatagcctaa acaatctgct tgtttcataa actgattcgt tgctgcccgc atcattttag
    28201 gattgccaac tggatctcct aaaacaaagc atttgtttcc tttgatctga taaccaaaaa
    28261 cgacttgatc ttggtcgtct tcttggtaat aaaaataact gtaacctgac aagcgaagct
    28321 gatggcttgt agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt
    28381 actcactgcc aagcttcgta gtatcttcag ctaaatattg ataaagcgcc actaaagcta
    28441 gcaatgagat gcccaaacca ataagccctg agaaccacac atcatctgaa ggaaacaaaa
    28501 taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg
    28561 cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa gattatagct ccccaagaat
    28621 aaacgaattt tgttcgataa aattcttttc tggataacca cacagctaac agaatcaaga
    28681 catataccaa tatcaactgc caagataacg tacgtgtgat tgtatttaaa ataccaaaag
    28741 atagtaaagc aatcgtaggc agaaatgcct ttttgacttt tactgaaagt gctctagcaa
    28801 ggccgagtaa taaaaagcca accaatagat tcactgtctg atctaaaaaa ttgaatgaga
    28861 acggcaataa tacttgaaat agccgactga ttgcagacag attagtgatt gttgatagaa
    28921 gtaccatcat gatccccgca aaatagacag cacttacgag cacgatatgc gcaacctttt
    28981 ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat tttgactcct gtatgtgtga
    29041 tgaacaatag aagtcccgtc ataaaaggta gaacataata aaacaaccga taatataata
    29101 gccaaactac tgctacatct tgccgaacag acaattgact cattcctaag atcatcaaaa
    29161 cgtcaaaagt ccccattcca cctgggacca tcgtcagcat tcctatcaat gtagcaatca
    29221 tgaacatagg atagatcgaa agcatcgaaa tatctattct catcaacttg ccgatgatca
    29281 aaaataccgc aagggctccc gtccattgtc caaaggatgc cagtagcaga ccaagcaagc
    29341 cttttggata aaactcgaag aacaatgtct ttctctttag ataagcaaaa aagaaaatga
    29401 ctggtgctaa aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa atactttctg
    29461 actgaataaa aaagacatca ataaatgtaa gaaacgacca tacagacaaa ccagtcagca
    29521 taaatagtgc gacttttgat acagtggcta aaaccatttt tctatttgtc tgctttccat
    29581 aaaaactagc ccgcagactt gctcctacca caccaccaaa accggcgaga ttgttgatcg
    29641 tgtttgttac ccacgcagca agaagaaact cttttctttc catttttggt tttccttgtt
    29701 tttctaagac tctgatcgtc acccaatcat acaacaacat cggagtgaca cccagcagtc
    29761 cagccagaat catgagcagg atcgtcatgc ggctttgttg tcccatcctt tgccaaatat
    29821 cttgccaact catgccatta gcaatatttg cgacttgatt tgccacaaag actaacacgg
    29881 agccgaaaaa aatcagtttt aaaatcagac tatgttcctt tatccattgc agcatcaagt
    29941 atcgtttatt tttcacattt tcccattcct aacttgtata cttttgtgtc ataccccaat
    30001 gttatcttat ttattttaat ttttccactt tagacctgtt ctttgcttgc gatgatagaa
    30061 aaagaaagat atcctaatgc taaaggagag gataacatga aagtaggatt tatcggcaca
    30121 gggaatatgg cacaagctat tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa
    30181 gaggtgttcg tttccagtgg acattttgaa aatgcacaaa aatttgctgc tgaaactggc
    30241 gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt tcttttagcc
    30301 gtgaaaccaa aaatcattga aaaagtattg gaagaactaa gagaatatag ccagaaagtc
    30361 ttattcattt ctattgcttc aggaaaatca ttaatagatt tagaaacagc gcttgaagat
    30421 cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt
    30481 tctgcgattt gcaaaaatga agctgccacg gaggaacagc ttcagcaagc tacgatgatt
    30541 tttgaagcaa tcggcagcgt ctatccttta gcagaatcag atttcagtac attcattggc
    30601 cttgctggca gctcacccgc attcgtttac atgctgatcg atgcgatggg acggactggt
    30661 gtactgaatg gtttgtccaa acagacagcc actgagattg ctgcaaaagc aattatgggt
    30721 tcttgtcaaa aattcctttc aagtgataaa aatccatggg aacttgtaga ccaagtttct
    30781 tctcctggcg gtacaactgt tgcaggtgta gttgccttag aagaagcagg attcatccca
    30841 gcaatcatca aaggaatcaa tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa
    30901 taatgatagc aatgtttttt cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg
    30961 gctcttcttt tgtatttttt attatttagc ataatcagtt gcacgagttt cccgaataac
    31021 gattactttg atgtgtccag gataatccag ttcttcttcg attcgtttac gaatatcacg
    31081 tactaaacga acagcgtcta aatccgagat ttcttctggc ttgaccatta cccgaacttc
    31141 gcgtccagct tgaactgcgt aacttgattc tacaccggcg aagctgttcg aaatattttc
    31201 cagacttctt aaacgacgga tgtagttttc taatgattca cttcgcgcac caggtcttgc
    31261 tgctgagagc gcatctgctg cagctacaag gacagagata acagaagtag cttccacatc
    31321 tccatgatga gaagcgatcg cgttgattac tactgggttt tctttgtact tggcagctaa
    31381 ttccgcaccg atctcaacgt gagatccttc aatctcatgg tcaagtgctt tcccaatatc
    31441 atggagcaac ccggcacgtt ttgctaattg gatatcttcg ccaagctctg ctgctaagat
    31501 accagctaat tttgcaacct cgaccgaatg cttcaagacg ttttgtccat aactagttct
    31561 gaatcgcaga cgtccgatga tcttgattag atctggatga agtgtatgag caccgacttc
    31621 aaacgctgcc tcttccccgt attcgcgaat acgttcatcc atttctttac gagatttctc
    31681 gaccatttct tcgatacgcg ctggatggat tcgtccatct tgtatcagtt tttccaaggt
    31741 catccgtgcg atttctcttc ggattggatc gaatccggaa agaacaactg cttccggtgt
    31801 atcatcgatg attaaatcga taccagtcag cgtctcaagc gttcgaatat tacgaccttc
    31861 tcgtccaatg atacgacctt tcatttcatc attcggaagg gtaacgactg aaacagtcgt
    31921 ttctgaaact tgatctgctg cgcagcgttg aatcgctaat gataataagt ttttcgcttt
    31981 gcgatctgct tcttctttgg cacgctgttc agattctttt accattagag ttaactcatg
    32041 attcagctct tcttcagtcg atttcatgat gacatctcgt gcgtcctctt ttgataaacc
    32101 agcaattttt tctagttctt tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg
    32161 ctcttcaatt aattgctgtt ttgaactaag tgcttcttct ttactttcaa gtgagttctc
    32221 gcgtttttct aacgagtcat ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt
    32281 ctcttgagat tttaattcta acttactttc tctcagctca ctttcaactt cagaacggta
    32341 tttctgattt tcttccttag cttctaacaa tgcttctttt tttagagttt ctgcttcttt
    32401 ttttgcacta tcgatgattc cagcagctga tgattgtgca ccagctattt ctttctcgtg
    32461 acgtgacttt gcgaccataa accctaaacc aagaccgaca attaaaccga tgatagcgag
    32521 gagaatgttg aataccataa aatccacctc catactatct ttttcatttt tatcatctat
    32581 acagcttcta tgataaatta ttctaaactc gtcaatatgc atcttcgcat atatgtgtac
    32641 aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac
    32701 aaagaaacgg ctttctactt attcattaaa cgatggtttt taaaataaat gacactagtt
    32761 tcatataggt aaacctttaa attgagaaac ttttctatta aattattctt tgtagatgaa
    32821 cagatatttt tattttatgt acccactcgt ataattaaat aactcacgat ttctttattc
    32881 aataaaaaaa cggcaggtca aggagagtac ctactcttca gaccagccgt ttttgtttat
    32941 cataattttt attcttctac taaaggcaat tcttcttgtc ctttttcatc ttctgggaca
    33001 gcgacttctt caccaatacc ataagctgct ctaactagag cagaaacttc tgccatcatt
    33061 tcaggatgat tagccatata gattttagcg ttttcccgtc cttgaccaat ccgatcttct
    33121 ttataagaat accaagcacc gcttttatca acaatgtctt tttctactgc catgtctaga
    33181 agttctcctt cttgtgagat ccctaaacca tacatgacat ctacttcagc gattttgaaa
    33241 ggaggtgcta ctttgttttt tacgactttg atctttgtac ggttaccaac aatatctgta
    33301 ccttgtttca attgttctgc acgacgtact tccaaacgga tcgttgcgta gaatttcaat
    33361 gcgcgtcctc caggagtgat ttctggattc gcaaacatca cgcctacttt ttcacggatt
    33421 tggttgatga aaatagcgat tgtttttgtt ttgttgatcg aaccagagag tttacgcaat
    33481 gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt cacccatttc gccgtcgatt
    33541 tctgctcgtg gaactaaagc agcaactgaa tcgactacta cgatatctac agccccactt
    33601 gatactaaag catcagcgat ctctagacct tgttctcctg tgtcaggctg tgaaagaagt
    33661 agttcatcga tattcacacc taatttttgt gcatattgcg gatctaacgc atgctcagca
    33721 tcaatgaaag cggccgttcc gccatttttt tgtacttctg caatagcgtg tagtgcaact
    33781 gttgttttac ctgaactttc aggtccatat acttcgatga tacgtccacg ggggtaaccg
    33841 ccgacaccta aagcgacatc taacgcaaga gagccacttg gaattgtaga gatttgttgg
    33901 tcgatttttt cgcccaattt cataatagag cctttaccat agctcttttc aatttttttc
    33961 agtgcagcat ctaaggctgc tttacgatca tctgccaata cgataatcct ccttatattt
    34021 acaaatcaga gcaacatttc attatcctta tcatatcttt tttagtgaaa aaaagcaaga
    34081 aaaaaccgaa caaatattcg catttatcat tttcttaaaa gctgttttct caaaagatca
    34141 aatccttgca taaccgcatt atatctgata gcagcacgat ctctggcaaa gtgatatttt
    34201 tccgaaacca cgccgtcttt agatgaaata gcgatccata ctgttcctgc agattgacct
    34261 tctaacgtgt ccggtccagc cactccggtg aaagctacac tgtaatctgt tcttgctatc
    34321 tcacgtgctt gaatggccat ctgctccaca cattctttgc tgactgttcc atattctgct
    34381 aatagctccg gatcaatctc cagaaaacca gcttttgttt ggagcgagta ggtaacgaat
    34441 ccgcctggaa atacttcaga tacaccagca atgtcgccta atgcggcttg gaaggcgccg
    34501 gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt ctttcagtag ttccactacg
    34561 acttttgcta gcgaattatc atcgccgtat ccataaaaat attcccctac tcgttcttgg
    34621 attttttctt ctaatgctaa aagagcttga ttccctgcct gaacatcatt tgttttcact
    34681 gtcagtcgta atgtcacttc atttggtttg gcatatggtg cgatcgttgg attgatctgt
    34741 gtctcaatca gatcttttag ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc
    34801 aaaactttag aaattagttt ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact
    34861 tgttccacaa acattggttt caactcatta ggcggaccgg gaagaaggat atacgcatgt
    34921 gtatctgttt gataaaaagt ccctaaagca agccctgtac gatttggtag aggaaccccg
    34981 ccttcgataa tctgggactg ttgcagattg tttttggtca tttttcgatg ggttgtttca
    35041 aaatatgcca ataatttttt gtatccttcc gtattttgta tgagagactt accgaggtga
    35101 gccgcagttg cttcttttgt caggtcatct tctgtcggtc ctaatccgcc acataagagt
    35161 atgagttcac ttcttgtttc agctagagca atcaactctt ctaaacgctg ctgattatct
    35221 cctacaactg tttgataata aacttcgata cctagatcag ctagctgttc tgacaagaaa
    35281 gtggcattcg tattcacgac ttgtcctagt aaaagttctg ttccgacagc tattatttct
    35341 gctttcatta cttcccgctc ccttatatag tatacgctgt atttttatat ttttttattt
    35401 aaaaagaggg caaggagctg tgacaaaaat cttgtcacag ctccttgttc caaataaaca
    35461 gtgttctcct gccaaaacct cggtcataag tcacaatata cataaaacat gaaaagctgt
    35521 tttttgaaat tatcccttgt gactcagagt tagacggcag gctcacaacc tctccactaa
    35581 acgatactta cattgatcct ttgaacacac cgctgttttt gatgaagtaa tccacacctg
    35641 aatagatagt aaagatcaga caagcataaa gcatgatttg atctactgga ataccgatca
    35701 ggctgaatgg gatattattg ataaacagca agataatagc aaccatttgt gtagccgtct
    35761 tgacttttcc cggccaagct gcagccatga cttctccatg ttcgacaagt agtaaacgca
    35821 agcctgtgac ggctaattca cgacagacaa tgatcgctac tacccaacca ggggcctttc
    35881 cttggtcaac caacatgata aaagcagtca taactagcat tttatctgcc aacgggtccg
    35941 caaacttgcc aaaattagtg acaaggcctc gcgcacgggc aatttttcca tctaaccagt
    36001 ctgtgatact agctaaagca aaaataactg cacctactag ctgtgtgaca gccagtgtgg
    36061 tgtctcctac gttaaattgt ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg
    36121 ggatcataca aattctaagt acagttaatt tatttggtaa attcacacta tcgactcctt
    36181 tcttccctat tctacgtgac tatcagcgaa gtttccaccg atattattta cttttccaaa
    36241 tgaatttatt caaaggagta tttttattga aattgatctt tttgaattaa cgaagaagga
    36301 ccgcgacaca gatttgtcac gatccttctt ttttatacct acaagtaaac agatgcgtaa
    36361 actaatttat acgatcattc tgcgtattga agagtaaggt tcacattttt tggactctga
    36421 ttgttttccc ctggattaac tggtacggat tctccattcg ctttgatcgt tgcattgctt
    36481 gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg gtaacacggt actctgtgtt
    36541 tcatttgctg ccagtgtccc ttgataaaca taggcattgt ctactagcac acctaaccat
    36601 acacgattga cagcagtaaa ttcaagggtt acaggcttgg tagcgtctgt gatatttact
    36661 gcgatggcac tgccagaatc ttctcctgta gctattgcca ttttcttttc ttcacttgat
    36721 tttgttgttg attctactgt agaagactct ttggtctgcg tgctttgctc tgaggaagag
    36781 gactcctcga ctgatccttc tactttaagc gaagaagctg tgtctccgat tattggatca
    36841 gcttgacgat cttgccaagt catatagccc acgacaacga caatcgccaa agcaaccaat
    36901 cctaataaaa tgacaggaag acttgtccaa aagcgacttg gatgcgattc ttcctgatgc
    36961 attgcttttc tagaagtact tactgtttca ggctgcggac gttcagggac cgatcctgtc
    37021 aatttgcttt tcccatcaaa aacatcgact aaatggtctc catcttctcc tacagcttca
    37081 gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag gcaattggtc aaaatcatca
    37141 ttgtcgattg cttccaaata acgtttttga atttttgtaa tctgctgtaa ttcatctaaa
    37201 gatatttttt tattcaggcg tgcttgccgt aatttttctc cgatggtttc aaaagccact
    37261 cgttcatctt ctccagtctg tttgattctc cgaagcatct gctgttgcaa cgaaagacgt
    37321 tatgtttggg tgctgtgaca aaagtcatgt cacagctcct tttttccgaa taaacattgt
    37381 tctcctgcca actcctcggt cacaagtcac aatatccaat aaatataaga agctgttttt
    37441 tgaacttgtt ccttgtgact cggagttaaa cggcaggctc acgacctctt tcttaaacgg
    37501 tgttcaatca gctgttcctc ggtattagct caaaaaccgg caaaatataa ggagatattt
    37561 ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac
    37621 ggtgttcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac attaagaagc
    37681 tgtttttact tggctgttct tattacttga tgcagggcag ctttttcaca acctcttggt
    37741 acacggtgtt ccagaagtag cttcttcggc aataagccga aatatcacaa aaatttgaga
    37801 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctctt
    37861 tgattacttc gtttgatttt attttaatat aagttgatta aaagagagca tgatttgttt
    37921 tattttctca ctaatttttc caacacaact aagaatacca taaaagctga ttttggagga
    37981 ggttcctact acaagtctaa aacgaaattt aagacttttc ctcataaaat actggtttat
    38041 tttggtctca tgtaaaactc gctgaaggcc tcttcactta taaatgcttc cccagctgcc
    38101 agtacgtctt tcatttggat cgaatcgatg atttcaggaa gatcaaataa agttcgatcg
    38161 ccaaataaac tttgcgtaaa ttgattggca atatattcga tcgaatttaa tgattggaaa
    38221 tattgtccaa gcattttctt cttcagcaaa tcgagatttg tttctgacac ttccggatca
    38281 tcagcgaatc caagaatgat ttctttgact ttttctgctg ctttctctgg ttcgtccgta
    38341 tctccgctga agtccgcaaa gtgaaattca cgatccaagc tgaattcaaa accaaagctg
    38401 tcatcgataa ttccttgatt gtacatcgct agatagtttc gagaagtatt gcctaaaatc
    38461 atttggaaca acagattgat tgctgtttta tagcgtaaca gttctgttcc ttcttgcggc
    38521 aatgtgtcta atcctttaat accaagtaca aatttatctc gtgtaatggc tgcttccaat
    38581 gcactttgtt tgatgatttc ttttgtgttt tcagggaaat aacgtacaat ttcttgcttt
    38641 ggagggaaat tttttgcttc ttggttttcc cgaataagtt tcatcaattt ctcaggctcc
    38701 atcttaccaa ctacaaataa aaccatattg cttggctgat aaaacgtccg atagcacgta
    38761 tacaagtctt gagctgtgat tttgtcgata ctttcaaccg tgcccgcaat atcgatatgc
    38821 aatgggtgtg taggatacaa gttatttagt atgccaaaaa acatccgcca gttagggtcg
    38881 tcttcataca tttggatttc ctgaccgatg attccttttt ccttgtttac agtttcttct
    38941 gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca gattcttttc tacctgatcc
    39001 gtcgtagaaa acagatagct ggttttcgta aaacttgtga aagcattggc ggaagctcct
    39061 tgtttcccaa ataattgaaa gacatcacca tcttcttttt caaacaactt atgttctaaa
    39121 aaatgggcaa ttccatctgg aactttcacc ttttcttttt caccataggg aataaattcg
    39181 ttatcgatcg aaccataatt cgtactgaac aaaccatatg tcttgtgata gtcattttta
    39241 ggcaacaaat acacggtcaa accgtttggc agtacttcat gataaagtgt ttcattgatt
    39301 tgctcatatt ctgttttatt catttgcatt tcctcctgct aaaaagaaga ttgcctgcaa
    39361 ttcgatttgc tcagctactt gttgtatctc tttgatcgtt acgctctcca tacgtttcaa
    39421 ccattcttca tcagataatt ttgtttccgg cagccagctg tccaaaaaag aagcttcgat
    39481 tgccgcttgc ggagagtcta gagaaagcag atattggttt ctcagcattg cttttgtctg
    39541 agacagttcc aactctgtta tttcaccatt tcgcaaactt tctaattgct cgtgaatcag
    39601 atgaagtact tgatttcggt tcttttcatc gataccggtt tggacactca tgaatcctcg
    39661 gaacgtatcc acgctactag aggcataata agctagactt tctttctctc tgacattcat
    39721 gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta aagaccatta gcgcaaaacg
    39781 ttctggttca tcgtaataca catttgtctg ataagctagg ttcagctttg cttgaacgat
    39841 tggctcacgt acttgtcgtt ccttcacgat attcacttga ggttgggtat aaaacagatc
    39901 tggataaact gtttcccgcg actcaaatgg taattgccca actgcttcag ctgctttgtc
    39961 cggatcaaca tcgccgacaa caaaaatgtc gatttgatca tttgccatca tttcttgata
    40021 gtaggacgct agcgaactag cagtgatctt gtccaaatct tcgactacgc ccaaccctgg
    40081 gatcttttgt tccgggctgt tttcaaaata aagttcttgc aaggcatagg aagcaaaagt
    40141 ttgtttatct tccttcaaac tttccaaata tagacgcaga ttgttctttt ccaagtcaaa
    40201 cgtcaactga tcaaactgat gatttttaat atttggataa aataaaacct cttttaaaaa
    40261 atctaccgct tgagaaaaaa ggtttggatc attgacatat tcaccattca caaaactgat
    40321 ccctgcgttt agccaatgta aatttccttt ttttctaact gataatccaa agcttgcgcc
    40381 ataaagctct gcaagttgcg cacttaactt tgtctgatcc gaataattca aactatttgt
    40441 ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact tcttgttgca gacgcgcagt
    40501 aaaacggata aaaatccgga tcgttttgta ttgttttgtt ggtaagacat gaagattcac
    40561 gccttctgcc aattgataca ccatatttat atgctccttt tcacagttga ttgcccgctt
    40621 actcttttaa tgggtcaacc tgcctttttt acttattcct ctgcttgaaa tttataagtt
    40681 caggcaattc gccgtttttt ctaaatgatg ttacacttta tagtatagca tagatgtttt
    40741 ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt tttatttttt atttttcgat
    40801 atactaaaga caaataaaag aagggagggt cattcatgat caaagaattc aaagagttca
    40861 tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt tattggtagt gcgtttacaa
    40921 gcatcgtaaa ccaagtggtg caaggcttga tcacaccact aataggttgg tttgtcgcaa
    40981 tgatcacagg tacttctgat ttagaaggag cactttcaat tcttgactgg tcgccgacaa
    41041 aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat cattacattt ctgatcactg
    41101 gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc gaagaattta cgctccaaag
    41161 aagagcaagt ggaagtggaa gaaactccta ctgcagaaga ttatcttagt gacatacgcg
    41221 atcttcttgc caaacaagtg gcagaagaca atacacatcc agtagagcaa acaaatcttt
    41281 ccgatacttc taaataataa agaatctaaa aaaaagaaag ccgattttca gtgtcggctt
    41341 tcttttttta gattcttaac ttgctacttg ttcctttttc atgtcgatat gtggaatatc
    41401 atcttccaaa tacacatctg agattgcctt gaatccaaac gactcataaa aagcttgtag
    41461 gtattcttgt gcgccgatca cgattggttt ttctggaaaa tttttctgga tcatgttaat
    41521 cgtttcttga acaatctttc ttcctaaacc atctttacgg ttgtcctgat gaacaaggac
    41581 acgtccaaaa tggatactct ccgcttcttc atatactcgc gtgtaggcaa ggatcttccc
    41641 atcagtatct tgaaaaaacg tatgccaagc ttctccatca atgtcatcaa cttcttgata
    41701 aggacactgt tgttccacaa caaaaactgc gattcgttct ttgacgatcg cgtaaaactc
    41761 tgctgttgac aattcttcaa atttctttac ctgatattcc actaagcaaa cgccccctgc
    41821 ttttttattc ttagcatacc gattcgcaca caaaaaagca aacgaacctt acgaatctca
    41881 ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga tctcaactgt ccgagtaagt
    41941 acatctgaat agctgtaaga tactcgttca aaagaatttt cttcaggatc caaatcaaca
    42001 acgaatactg aaggataggt ttctgttaaa acgccgcggc gttctgtttg acgctttctt
    42061 cctgtctgag caaccaacat gattgggctg ccgatacgac cttctaaatc tttcttgatt
    42121 gaagctaaag ttgttggcat acagttcacc tctactatca actataccac atatttttaa
    42181 aattttcaag tttaccataa aaaaaaaaga aatgcaataa aaataaataa gaagctcatt
    42241 aagatttttc taaattattc ctcattataa aatttcaagt gtttttttat tttgagtttt
    42301 actctcccgt aagcgctcat tacttgcttg tgatccattg ctaaacattc agccatgtct
    42361 acaaaatcaa cgccttgcat ataataatga aaaattttag cttctatttt tgaaaactgg
    42421 atttcgcaat tttcgattgc ttcttttaat aaaagatgct gaattgctaa aggttcttgc
    42481 aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc gattgagact
    42541 tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga atagatttga
    42601 ttgcttataa cgcttttata ataacttcca aagctaacac ctttgctttt ttcgtaagtt
    42661 ctcaatgcct gatataaagc gatccttgct tcttgttccc aatcctcaga aacataattt
    42721 cttaaataat aggtgttttt caaacgtagt aaaagtggaa aatatttttg atacagggca
    42781 gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata actcctcaga cataaaataa
    42841 ttcctccata aattcgttta tggaggaatc ttatcatcaa tgattcatcg aactgattag
    42901 aacgatatgt tatttatttt ttctcattaa atcgtccaat ttttgggaca atttttttaa
    42961 ctgctcttca ttccatggag aattacgtcg gaaatcaata aaatgcatat ctttattttc
    43021 ttgggctatt gttttttccg ttttttttat cgtcttatat aattcataag cagaagttcg
    43081 taatgctcct tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg ccactgtcac
    43141 ttgtgtcaaa cggtcattca gctgaccagc gattctctcg atataactgt cagccgtttc
    43201 gtcttctttc gtaaaaacaa catccaactg gtatttctta taattttgtt ggatgcccgg
    43261 aactagttgg gcatcgaaga caacgatgac ttccattcct tcatatttcg cataatttga
    43321 taaacgatgt aaaagagctt ctcttgcatc cgccattttg tcctgctttt taagctggac
    43381 aagttccggc cacgaaccaa tcatgttgta gccgtctaca atcaacagct gttttttcat
    43441 ggtctactcc ccctgacgtt gggaaaaagc ctgatacata agcaaaccag cagcaacact
    43501 ggcatttaag ctctggacgt gcccggtcat cgggatcgtc agcaattcat ccatttcttt
    43561 tttcaatcct ggactcattc ctttgccttc attaccaatg actaaagcaa ttgatccttt
    43621 ggcattccat ttttggaagt ttgtgccggt catatctgtg ccaaataccc aaaatcctgt
    43681 ttctttgagc tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag ggacatgttc
    43741 gactgcccca gtggaagtct ttgttacgat aggtgtgatt ccaactgctc ggtgttttgg
    43801 tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc caaaattgtg
    43861 tggatcttcg atgctatcca aaatcaaaaa gaaggggtct tcttttttct cttttgttcg
    43921 ttcaatcagt tggtcaaggg ataaatattc ataaggtgta atcgcaagaa cgactccttg
    43981 atgcacgccg tgatcgctca aagtttctaa tttttgttta ggaacccatt tgactggcac
    44041 agcttgttca cgtgcgattt ctttcagttc ttctactttt tctcctcgtg aatcttcttg
    44101 aagaaagagt ttattccctc gcccttgttg tagcgcttct actgcggcat ggtgcccaaa
    44161 gacgaaatta tcttctaatg cttcttcatt ttgatcagaa actgactgat ttcgtccagt
    44221 ttgcttttta tttgaatatc tattttgttt tttgtcttca aatccacgtt tcgattgatg
    44281 tttgtttgtt ctttctttac ccattttttt caccaacttt ctgaatacac cagcggatca
    44341 attcttccat ccgctcggtt tgttttgtca aatgcagata gcccatgagc gcttcgaacc
    44401 ctgttgcgac ccgatacgtc gtaacatcag cattttttgc gctcgtatgg cttttagcat
    44461 ttcttcctct gcgataataa agttcttctt cttctgtcaa aagatgttct tccagcattt
    44521 gctgaatcaa aaaggcttgt gcattcgcag aaacataatt cgttgccgca cgatgcagct
    44581 tattgggttt tgtttctcct tgatcaatca ggtggtcacg aacatagatt tcgtaaatcg
    44641 catctccaac ataagccaag gctaaaccgt tcaattgtgt gtaatctctc atgcttctct
    44701 tctccatcgg gttccttgcg gtgtatcttc taataagatc cctttttctt tcaacaaatc
    44761 acgaatttcg tcgcttcgga caaaatcacg attgcttctt gcttgatttc gttcttcgat
    44821 caattgttca atctcttcat ctaataactc ttctgctgta aataaaatac cgaagatcga
    44881 aagccattgc gagaataaag cttctccttg tgccaagaca gcggcagaaa ctttcggctg
    44941 gtctagataa gtgttcagcc acttagcaag ctcatagacc actgtgatcc catttgctgc
    45001 attaaaatca tcgtccattt cttcgatgaa tcgctgttct aaattttcta acaaagcgat
    45061 tttatccgca tcatctgcca aattctctga ggcagtttct tggcggaatt tcaaattgtc
    45121 aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg tttcactgta
    45181 gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt gtggatcaac
    45241 gtttttgatc atttcatgta cagtgatgaa attccctaga gatttactca ttttttcatc
    45301 gtcttcgcca atcgttacat agccattgtg catccagtaa ttagcgaatt ttttccctgt
    45361 tttcgcttca ctttgtgcga tttcattttc atgatgagga aattctaaat cttgcccgcc
    45421 accatggata tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac attcaatatg
    45481 ccaaccggga cgtcctttgc cccatggtga atcccaagaa atctcgccag gttttgcttg
    45541 tttccataaa gcgaaatcta atgggtcttc tttttgctgc tgttcttccc ctgtacgctg
    45601 acttgctccg acttccagtt catcgatgga ttggtcactt aatttcccat aattttcaaa
    45661 tttacgggta cggtaataca catcacctga agattcataa gcatagcctt tttcaaccaa
    45721 cacttgaata aatgctaaaa tatctggcat atgatccatg actcttggat gcagtgtagc
    45781 aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac gatcagcaac
    45841 ttctggtgct gtaataccta attcgtttgc tgtacggatg attttatcgt ctacatccgt
    45901 gaaattcgat acgaaatcga ctttatagcc tcgatattca aaatagcgtc gaattgtatc
    45961 gaaagcaatg gtgctacgag catttccgat atggatataa ttatacacag tcggtccgca
    46021 gacatacata ccgacatgtc ccttttgtat agggatgaat tcttcttttt ttctggtcaa
    46081 tgtattgtag atttttatca ttctgatctc ctcacttttt ctcctttgat tcgaacgact
    46141 tttgctggta ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac aacggcacct
    46201 gcaccgattt tggcgttttt tccgatcgtt accggtccta atatctgcgc attcgcagag
    46261 agcatggctc cttgtttaac agttgggtgt cttttacccg tttcttttcc tgtgccgcct
    46321 aatgttacgc catggaataa aatcacatcg tcctcgattt ctgcggtttc gccaatgacg
    46381 attcccattc catggtcgat gaacacgcca gttccaatcg ttgctcctgg atggatctca
    46441 acaccagtta ggaaacgcca gaattgagca ttgatcttcg ccaataaaaa gagccggtgc
    46501 tgatataaaa aatgagagaa acgatgccaa aacaaggcat gaaaaccagg gtatgtcaaa
    46561 acgatctcag ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc aatcgctcgg
    46621 gtaaaccaac tcatgacttt ccactccttt cgtaagaatt ttatgtagga aatcatcttc
    46681 ttataaagaa aagaagcgcc ttttaaataa gtttccttat caaaagacgc tttccgcgtg
    46741 gttccacttt tcttcataat caatgattat ctctgacagc taacgcctgt tatacgttct
    46801 tggctacttt gtttcctata aaacagtttt tcctgttttc tgtgtttcgc caagaccact
    46861 cccagaggca tttcggcaac cattcattga taccttgcac cacccggtat ctctcttgta
    46921 atgatcagct gcttactttt ttctgttcat cgtgtttagt tcagtacttg atttaaatgt
    46981 gctaatgatt tttcacgacc aagcaattcg atcgtttcag gcaattctgg accatgcatt
    47041 tgtcctgata cagctacacg gattggcatg aaaagatttt ttccttttac gccagtttct
    47101 ttttgaacag ctttgatggc agctttgaca gttggagcat ctactacttc catttgttct
    47161 agctgtgctt tgaatgcgtg gagaacagta ggaactgttt caccagcaag tacttctttt
    47221 gctgcttcat tcaatacagg atgttcattg aaaaatagcg tagacagatc aacgatttct
    47281 gcagcatagc tcatttgagg ttgatacagg ctaacgatct tcttcaccca ttcgattgtc
    47341 ttttcatctg ggttttctgc tacacgtcca tcagcgatta gataaggcag acacatgtca
    47401 gtaaatgcat ctagatccat tgctttgatg taatggttgt ttacccattg cagttttttc
    47461 gcatcgaaag ctgcgggtga tttacttagg cgttttggat cgaacatttt gatcaattct
    47521 tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc aataaagttg
    47581 aacattgcat ctggtagata gcctaattcg cgatattgtt cgataaattg caagattgat
    47641 tcgtcacgtt tgcttagttt tttacctgtt tcactgttga tgatcaatgt catatgtccg
    47701 aaggttggtg gtgtccagcc aaatgcttca tagatcatca gttgttttgg tgtattggca
    47761 atatggtcat ctccacgtaa aacgtgagtg attttcatca aatgatcatc gaccgcaaca
    47821 gcaaaattat aagttggcat accatcgcgt ttttggatga caaagtcgcc accgatatta
    47881 tcggattcga acgaaatagc gcctttcacc atatctgtaa atgcgtattc tgtattgcga
    47941 ggcacacgga aacgtacaac agattctaga ccttgtgctt ctttttctgc ttgttcttca
    48001 ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc gcgttgtgct
    48061 tcccgttctg cttctaattc ttcttctgtg caatagcatt tgtatgctcg attgctagca
    48121 agcagctgat caatcagcgg ttgatagatt tctttacgtt cagattgacg gtaagggccg
    48181 tattctccag gattttcagg agattcatcc caattcattc ccaaccaagc aaggttttcc
    48241 aactggcttt tttcgccatc ttcgatatta cgtttttggt cagtatcctc aatacgaata
    48301 ataaagtctc catcattgtg gcgggcaaac aaataattga ataatgcagt tcttgcattt
    48361 ccaatatgca ggtgcccagt tggacttggt gcatagcgta cgcgtacttt cgtcattttc
    48421 ttcctcttct ttcaaactag actttgacta acagcaaaaa ttccgtcagt tacggcttct
    48481 caaatatcat ttatttaaag ccactcagca caaattttac cttttctaag gtaaatagta
    48541 aagccaactg gttgaatttc cttaccagtt ggctgaattt cttttttatt ttgattgttt
    48601 agtctttttc tctgtctctt ttttattgtc gattcctcgt cctgcatgtg caggtttagc
    48661 aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt acaacgactt cgatacgttc
    48721 attcatgtaa tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa ggtaggctac
    48781 tccttgttgg cgttctgtcc cggcttttac tactagaaca ttcatcgttt caccagggat
    48841 gacgactggt tttacggcat ttgccaacgc attgatatta agaacaggta cattttggaa
    48901 ttcagagact ttattcaagt tatagtcatt ggttacaatg acaccatcta aaagcttagc
    48961 caatttgatc aatttgctgt ctacttcact gatatcttca aagtctccat cgtacatttc
    49021 gacagatata ccctcttctt tttgcaaggc atttaggata tctaatccgc gacgaccacg
    49081 cacacgtttc aaactatccc ctgagtccgc gatgtattgt agttcgtaaa gaacaaaatt
    49141 agggatcatc aaggttcctt ctaagaatcc agtctttgta atatcataaa tccgaccgtc
    49201 aatgatcacg cttgtatcca agattttata tttatggaaa ttttctcctg attttcgttc
    49261 taacagttga ctctcaactt gactagctgt ttcttgctcg atttttttgc ttcgtgaacc
    49321 aaaaactttc ttccattctt cgattcgtgt cgttcccatt ctcaatccca gatagcctaa
    49381 gatgatcatc agtaaaatcg gcaatacact attgacgaat ggaatttcca tattgtaaag
    49441 aggggtcgag atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg caccgaagag
    49501 aagataagtc aagctgacct cacttagttt ttgttctact tgtttgataa tagctgagat
    49561 atgtttggcc aacagcaaag ataataaaaa gaaaataagt gcgcctaaca ggctgttggt
    49621 aaatgtatta ttcagccatc cgtttgactg ttgaccgatt gcctgccaag ctgagggtaa
    49681 aaatgaaata cccaagcttg cgccgacaat gatcatcagc aaagtaatga cacgtttttg
    49741 catcgttttt cctccgtttc gacaatcatt tattagttag cggaacacac gcttcaacgt
    49801 ttcagccaaa gttgctactc caactatctc gattcctttt ggcgcttccc agttacctaa
    49861 gttgttttta ggtaggtata cttttgtgaa gccgagtttt tggacttctt tgactcgttg
    49921 ttcaatatga ctgacacgtc tgatttctcc agtcagtcca atttcaccga tgaaacattc
    49981 cgttggctgt gttccttttt ctttatagct tgaagcaata ctgacagcga tagctaaatc
    50041 aatcgctggt tcatttaatt ttaccccacc tgcagctttt aagtaagcat cttgattctg
    50101 aagaagcaac ccagctcttt tttccagcac tgccatgatc agagataccc gattgaaatc
    50161 aagacctgtt gttgttcgtt tggcatttcc gaacatagta ggtgtgacta atgcttggat
    50221 ctctaccaaa attggacgtg tcccttccat tgccacaacg atagcagagc cagttgcacc
    50281 gtctaggcgt tcttctaaga aaatctgtga aggattagcg acttcttcca atccatgctc
    50341 atgcatttca aaaatcccaa tctcattcgt tgagccaaat cggtttttta ctgctcgcaa
    50401 aatccgaaaa ctatgatgtt gttcaccttc aaaatataac accgtatcca ccatatgttc
    50461 taacattctt gggccggcga tcgagccttc tttggtaaca tgaccgacga taaaaatggc
    50521 tatcccattt gttttagcgg ttttgagtag ttctgcagtg gtttctctta cttggctgac
    50581 acttcctgcc acacttgtga tgtctggctg cgtcatcgtt tggatggaat caatgataac
    50641 ataatcaggg gagatatgtt cgatggcacg actgatttcg ttcatatctg tttcagcgta
    50701 tagataaaat tctgtgttga tacttccgag gcgttccgcg cgtaatttta tctgctctgc
    50761 gctttcttcg ccggatacat ataagacttt tccgcctatg gctgctaatt gctgagaaac
    50821 ttgtaacagc aaagtggatt tcccgatacc tggatcgcca ccaatcaaga ccatagaccc
    50881 tggcaccaca cctccgccta acacacggtt cagttcttct aattttgttt tgatccgagg
    50941 ttctttttta gggacgacgt ctgcgatttt tgttggtttt gctttttctc ctgtcaaact
    51001 tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg tattccattt
    51061 tccgcaattt gggcagcgtc ccaagaactt aggcgacaca tacccgcaat tttggcaaac
    51121 aaattgtact tttgattttt ttgccattgt accgcctcct tttatcctaa tcatgagaca
    51181 ttaaatcaag cttaagtatc tttaacgttt tatttactgg aagaaccaaa tccgccttga
    51241 cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat cccttggccg
    51301 atacgctcgc cttttttgat cactacatcc ttgaaaccaa agttggtgaa ttggaacatg
    51361 atatggcctt catttgcctc attattgtaa taatcactgt caatcacacc tactccgttt
    51421 gtcaacacta aaaaccgctt caacggattg cttgaacggt tagcaagctg caaaaattca
    51481 tcttctccca tgtaagcttt gattccagta ggcacgaggg ttggtttcaa ttgtttttca
    51541 ttttctgata catctttttt caatccatct gcgactgttt ccaacccctt tttccagcct
    51601 gctttccaga tacttggaat cacgacctct tctgctgctt caaaatcata gccagcagca
    51661 tgatgtgtag cgcgctgcgg tagattgata ttttgatctt tatatttaga aataacttca
    51721 aatcctcttt ttctcaccgg ttttcctccc taaataaact actcctattc taacatatcc
    51781 gaggctgtaa aagactttct ttacaaattc ttcaagaaat tctgctatct taaagtaaac
    51841 cattaattcg ttctttttcc aaacgactgc aagttgtaca aaaaaactat tttttacatg
    51901 actttactcg tcaaaacaag tagaatagaa cgaaattaat gagtggatat ttgattgagg
    51961 tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta ttgtcaaagt aggaacaagt
    52021 acattgattt atcccaatgg aaatatcaat cttgcggcaa tcgatcagtt agcttttgtc
    52081 ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag tctcatcagg agctgtaggc
    52141 gttggtatgc ataaattaca tttgtcagaa cgtccgactt tgattcctga acagcaagct
    52201 gcggcggctg ttggtcaagc cgaactgatg aatatctata atcaaagatt cagtgtatat
    52261 aatcaacaga cggcacagat cctcgtgaca agagacgtgg tagattatcc tgaaagtcgt
    52321 aaaaatgtga ggaatacatt ggaacagctt ttgattatgg acacgatccc tatcgtgaac
    52381 gaaaacgata ctgtagcagt ggatgaatta gatcatctga caaaatttgg cgataatgat
    52441 cagctgtcag ccatcatggc aaaacttgtc gcggcagacc ttttgatcgt cctttctgat
    52501 atcgacgggt tttattcaga taatccgact acaaatcccg aggctcagat gtatcgctat
    52561 atcgataaaa tcgatgaaaa gattctggca caagcaggtg gtgctggtag cacatttgga
    52621 acaggcggta tgcagagtaa attaaaagcc gcttctagga tatttgaagc aaacagcgca
    52681 atgattttag caaatggaaa aaatccggca attatttttg atattcttgc aggaaaagaa
    52741 gtaggtactt tatttaagga ggacttgaaa tgactgactt gatgcaatta ggacaacagg
    52801 caaaagatag cgcgcaacaa ctagcactta tgcctacaaa ccgcaaaaat gagctacttg
    52861 ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat cattgaagca aatcataaag
    52921 acttagaaaa agcagtcgaa aacaatatca gtgaaacgat gcaagaccgc ttgcagctga
    52981 cacctgagcg aatcaatgcc atggctacag agatcgaaaa aattgcttcg ttagacgatc
    53041 ctattggaaa agtagatgag atgtggacga acaccgacgg attgagaatc ggcaaaaagc
    53101 gcgtgccact aggcgtgatc ggcatcattt acgaatctcg tccaaatgtc acgacagatg
    53161 cagctagtct agcattcaaa tcagggaatg ccgttatcct tcgcggtggc aaagaagcat
    53221 tcttctcgaa tcaattatta gtccaattat tgcagcaagt actggtcagt gctggcgaat
    53281 ctccttatgc catccagttt gtcgatgata cttctcatga aactgcagga cagttgatgc
    53341 gattgacaga atacttagat gtgctgattc ctcgaggcgg agcaaaatta atccaacgag
    53401 tcaaagaaca ggcaacggta cctatcattg aaacaggtac aggaaataat cacgtatata
    53461 tcgataaaga agcacagtta tctatggcag tgaacatcat cgtcaatgca aaagcttccc
    53521 gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca ttctgagatc gcaccttttt
    53581 tcttgccagc tattgaaaaa gaactggtag aacacggggt ctctttacgt gcggatgctc
    53641 gtgcgttaga atatctagag acggctgctt tagcagaaga cacagattgg gatactgaat
    53701 atctagatta tatccttgct gtcaaagtgg tcgattcttt ggatgaagca attgcacata
    53761 tcaatcggca taatactaaa cattcagaaa cgatcgtgac agatagttat gcggctagtc
    53821 aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt caatgcctcc actcgtttta
    53881 cagacggatc ggtatttggt tttggtgcag agattggtat ctctactcaa aagttgcatg
    53941 cccgtggtcc aatggggtta aatgaattaa cttccacaaa atatatcatt tatggcgatg
    54001 gacaaatccg cgaataatgt ttcgtgtgaa acattattcc cgccttttgc cttttttctg
    54061 taaaggttct atccaactag aaaggtgtca atatgtttga aattttaatg atttttttta
    54121 tttaccttat ttctctgaat atcgctgctt ttctgggagt cggtatttta tcccttttct
    54181 ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa atggtcgcag tattttgaca
    54241 aaatcgggca aaaagggctg gttacacgct tgcacattag ttacatggtt gcattatgct
    54301 tattagcgac aatcaattac tattcttttt tcgatcattc aatcgcttat acgattacgc
    54361 tactgattgc tgggattttt cacttatctt ataaatatca gctgaataaa aaccacttga
    54421 atcggacttt ccgctaaaaa atatccgtac gataagtaga gtgccttgaa gatcggcaaa
    54481 aaactgccgg actttttaga ggctgtgaca aagattttgt cacagcctct ttttccatat
    54541 aaacggcgtt cgcctaccag ctgcctggta aatctgtttc ctcttcgtta agaagcgaaa
    54601 atccgtcaaa tgtttcgcca tcataataac gaagcagctc atccatggac aagttcatcg
    54661 ttttttcttt ttcttcttcc gctgattcag ccagtagctg ttcccgatac acctgataca
    54721 taccagctat tttttgctgc atcgcttttc tagtttcacc ttccgcattg attattggca
    54781 attctttgat atagccacga taaatcaacc gatcatctga aaattcaatg ggtaattcaa
    54841 cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat ctattatccc cccattcttt
    54901 ttcttaaatg aattttagct tacctactga aaattttcca gtttcattat tttgagaaaa
    54961 aaagggaatg tgacaaaaat cgtgtcacat cccctttttc cgaataaatg gtgttcttct
    55021 gccaactcct caatcataag tcacaatatt caacaaacat gagaaactgt tttttgaaat
    55081 tgttccttgt gattcagagt taaacgacag tctcacaacc tcttggtaaa cggcgttcaa
    55141 tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata tgagaagcta tttttccgtt
    55201 ttgtttctct aatacttagg ataaaacgct gattttacaa cctctgttaa acggtgttca
    55261 atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccgt
    55321 ttgtttctct aatactcagg acaaaacgct gattttacaa cctcctgata aacggtgttc
    55381 aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagaa gctatttttg
    55441 tttggctgtt cttatgactt gatgcaggac agctttttca caacctctga tttcttattc
    55501 ttcttcattt gtagcaaaat aattacggtg ataaatgata caacatacac tgataagaac
    55561 aatggcacct gcaaaataaa acgcatagct ttcaccaata tgttcaccaa tcacacctgc
    55621 aataaatggt gctaaagctc caccgaaaaa gcggacaaaa ctatagctgg acgaagcaac
    55681 gtttcgttgc agacctggta tttccatagc aatcgtcgtc aacagtgtat tgataattcc
    55741 ttggaaaaat cctgctaaca caatgctgat cggtacaagg actggcatag aagttccagc
    55801 tcctaataac agcaaacaaa taatgaataa gacaaatgct gctaaaattg tccgataagt
    55861 cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa gcaatagcca gaagcacacc
    55921 ccagccaaaa aatacaaaac cgacttctaa ttcagaatag ccaatcaata aaaatggcgc
    55981 atacgcaagt aaagtaaaga atccgaaatt atataacaat gcgattagtc caactgaacg
    56041 aagtttatga ttgcttaacg ctttgacacc ttcccaaagg cctacttttc tctttggttt
    56101 ctcagtttca ggtaaaagta ccgctagggc tataaaaccg caaaacatca aaaatgctac
    56161 cccaaaaaat ggataacgcc atgaaccact gcccaagacc ccaccgacta gagggccaac
    56221 agccattccg cagccaagtg ctgcttcata catcatgatt gctttttctg tgttaccaac
    56281 taaaatactg acgatcgttg ctaaagcagt agagacaaat aaagcattcc cgacacccca
    56341 gccggcacgt aacccaatca gcattccgat attcccagat aatccgccaa gaccagcgaa
    56401 tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc aaaccgatcc gactagataa
    56461 gaaacctgta aatagcatca ccacaccagt gactaacatg taactagtaa ataacagggt
    56521 cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg ggtctactag
    56581 acccactccc ataaaagcga tcatacaggt agctgctaca actagcgctg aacttttccc
    56641 ctttgtttct tcatttgttt gttcttgtat atctatttga ttctccaata tttccatccc
    56701 tttctctcta ttagcatcat acatgattta ttttacatgt caatatgcac atgtaaaacg
    56761 tggaaacttt actgtgatat aataaagaaa ataagacgag aggtaggatt tttatgaata
    56821 ctttagccta tatattatta tgcgtgcttt tatccaaacc acgttctggg tatgaattga
    56881 agcagctgat cacaattttt tgggaagccc atcacagtca gatctataca acgcttgcca
    56941 agttagacca gcagggttat gtgaccgttt tggacaaaga cgagcactct caaaaaaaga
    57001 tctaccactt gactgatgaa ggacgtgctc ttgtagagga atggatcaaa gaggaaactc
    57061 ccgctcctac gcaaaaagat gaatttctag caaaaatcta tgctttttcg acgctcgata
    57121 aaacaacagc tactggactg ctattcacta gagaacaaca gctgaacaaa atcatgcaaa
    57181 aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa acaagaattt ggacgttatg
    57241 ttgtgatcca gcgcagaatt atgctttgtc agcaggaact ccaatggtgt cagcaagtaa
    57301 gagaacaaat gcagcagttt tttgagtaag ggataaacag aacctttcta gaaatatcta
    57361 ttgaagagca aattatcttc aataaaagag caatacagtt ggaaaagaaa aagcgatccg
    57421 aacgacaggc caaaacctat cgttcggatc actatttttc tagaactgca ttcaagagac
    57481 tatgacaaaa agcatgccaa ggcctttttt caaataacag tgttccatca ttcattactt
    57541 tgaattgctt ttagcacact tcttgaaagc gggccaacga tgatcaactg caacggtaaa
    57601 gcggcaatca cattcatttt ccaagcattc atataagcag agaataattg atcaggtatt
    57661 ccttgttcca tgatcacacc gaataaagac atacatgtca ccattcctaa aaccatcaag
    57721 catgaaatag ttaagatcat ttggaccttg ctttctggat ttactggtag tttaaaggca
    57781 atttttttcg ccagcacacc gatgatgaag acatccaaaa tgaaagccac cacgaatcca
    57841 ggcactagac caatcaacaa acttgataaa tgaagatctt gatgcagcca taaattatac
    57901 gcgctcatcc ccaagaccat caaaaaacac atcattgttg taaaaattat tccttctttt
    57961 ttatttgtag gcaatctgta ccactccttt aaatttgtca cgaaaatcat cgtagcacat
    58021 ttaaaaattt ctcgtaagta ctttttttat tttctgccct tttatttcat aaacttcatc
    58081 agccgtacgc tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc ccggatatat
    58141 ttgcattagc ttctctaaag cttcgatcgt tgccatatca ataaaattag tcggttcatc
    58201 taacagcaac acattacttg gcttagtaaa aagcaaagca atcgtcagac gggtcgcttc
    58261 tcccccactt aatgtacata acggtttcgt caaagtttcc tgagcaaacc ctaagtgatt
    58321 cagcaaacta cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa aacgaatgat
    58381 ggattcttca gatgtcattt gataaccctt ttgctgatat acttgaaagc ttactttcgg
    58441 agagagaagg attccttctc cttgtttgcg gatatgttct aataaggttg tcttacctga
    58501 accattttca ccgacaatcg cgattttttt acctaaagaa aactgaaaat caccatttac
    58561 cagtaatgtt ctcccacttc tttccaattg gacattttgt cccatgatgg gatagcggct
    58621 gtggagttca agagattttg gtactggaaa acggatttgt ttcatctgct gcggcttggt
    58681 tgtttctcct attttttgga gtcttctctc taatgttttc gcattctttt ggatggcttt
    58741 ttgtacactg tctttttgtt tagaggaaga caaccgatcc agtctgattt gttgcttttt
    58801 ctttttactg gacacttttt ccaacttttc cgcttgtttt ttcttttctt ggatcgattt
    58861 ttccaatcgt tttttctctt tcatgatcga ctctgcttct cgtagctgag tctttttctc
    58921 caattccttt tgagtgagat aggcactata attccccgaa aactctcgga tttctccgtc
    58981 ttttacttcc cagattttcg atgctagctc atctagaaaa tagcgatcat gactaacaaa
    59041 aaccagcgta ccataataat aacgaagctc ttcaatcagt cgatcaatac tttttctatc
    59101 tagatgggtt gttggctcgt ctagaagcat cccttgttca taagaagaaa gaagacgtat
    59161 caactcgatc tttcgttctt cccctccgct ccatttccgc tctgaagttt cttccagtct
    59221 taactggaaa tgactggcta attccaaatt taggtccttt gcttcagcga gataggttaa
    59281 ttgaggcagg tagttgaagg tgatctctgt ttggattttc ccatgatcgg gaaaaagttc
    59341 accggctatt agtttgagca aagtcgattt accgacacca ttttctccta tgatccctat
    59401 acgatcttgc tgatagacac gaagagaatc aatagatagt tctagctggt tgccaaagct
    59461 gacttgtaga tttgttatat ttactgctaa attttccata tgttttacac tcctatttca
    59521 tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa atctgtttgc aaaaaaactg
    59581 catcacatct tttcctactc tgaacgaaat aactatgact aaatcaccaa atctgtcata
    59641 gctttcacct attctactgt cgttcgaaaa aatggacata ctccatccat gaaattgttg
    59701 tttatacatg tcttttgaat ttatttagtt gttattcaaa agttcgaaac gtaatttcat
    59761 cgatgcagtc atttttccga cctccttttt tattaggtta ccacacacga ttcaaaaaaa
    59821 gcaatagcta ctgaatagta actgaaaacc ttttattcga acatagtgat cttttctggt
    59881 gctaaacgaa cagactcgta accttcttct accgcttttc ctattgataa aataataact
    59941 ggaacataac ggtctttatc caaaccaaat gcctctgcca actgatctgc ttcgaatcca
    60001 ccaattggat ttgtttcata gccatgatca cgcgcaacta acatgaattg catagccgct
    60061 aagctagaat cgactttgac tacatcgttc atttcttctc ttgaaaaact cttgtaataa
    60121 ggaatgatcg cagctaattg ttgatctctg acttcttttg gcatcttccc tgattcgtaa
    60181 gcttggttgt agatttcttc gccatattcg taacattgca tatcgccaaa aatcatgacc
    60241 attgcagaag aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg caagatttct
    60301 tttgcttcct cgctttcagc gaccacaaag cgccatggtt tcatattgac agaagaaggt
    60361 gcagtcgtcg cttctttgat catttccagc atttcttcgt gtgggatttt gtatgtttca
    60421 tcatatacgc gaacggatct tctaccagtc ataattgttg aaaaatcatt tttttgttcc
    60481 attattatta acctctttcg tttctgttgt tatattgcaa agttgggtat tcaatttatc
    60541 taaaagtgtt agaaattgtt tcatttctac atcgctgaaa gccggggata gcacggaacc
    60601 tgtatcttta tggtccattt cacaatgctt gattttcttc tgggcttttt ctgttagcgc
    60661 tacatacact tcccggttgt tctgaggatt tctctgacgc gaaacatagc catttgcttc
    60721 caataccttc agatgacgag taactgctcc ttgatcaatc tgcaactttt cttgcaaact
    60781 gctttgtagc aatggatttt cttctttcag tctcatcatg atctcgtatc tagtaagact
    60841 tataccgata ttttcttcaa agatctggac gattttttga tcggctaatt tgatttgata
    60901 taaaaggtga ctaatctctt ctaaagacat aatttcctcc tttctaataa ttgactcgtc
    60961 aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat cagctgtgta
    61021 ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga aattttttgt agaatgaacg
    61081 gaaaaaatga taaagaaagc acaacgctaa aaccaagatt attgattctt atcaaactat
    61141 agaagatagc tcttctgcag tagataattt tacaccgcca aaaatttccg aaatcgcctt
    61201 ttgcaagaat cccgtcgaat attagaaatt ggatcagtat gcttaaacag taataaaaaa
    61261 gttatgacaa gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa acttgcatct
    61321 gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg gctgttctta
    61381 ctacttgatg cagggcagct ttttcactac ctctttttaa taaccataaa atcctattcc
    61441 ttcatatctc cagcccatcg gtgttagcca atctctttcc ttagcactca aggtatagtg
    61501 atgggtacca gttttttcat ttggattaaa tagtcgataa attggcttgg aaccaccaga
    61561 ataaaaactt agaccttcat aacgccaacc gatttttgtt aaagagttga tctcagattg
    61621 tgccattgtg taatgatggt ctccactgta aggattgtat aaacggtaga caggttgtcc
    61681 gctgtttggt gcttgccaag caatcccttc atattcccag cctacagttg gcagataatt
    61741 tttttctccg gcattcaatg tatataaatg ttctcctgta tgacggttat acagacgata
    61801 catcggagaa gttgaagttg gtgctgtatc atactgtgtc agattatagg tttcgattac
    61861 attgttcaat gtagaaccat aattaggcgc agtggcataa cgacctgtta gccaagccgt
    61921 agcatctttg taagagttcg tattgctttt ccatgcaccg gaatagtagt aaacgcctgg
    61981 ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc aatgattctt tgtacgaagg
    62041 atattttcta aaagcggcat tcaccgtaat ccattgccca ttcacatatt ccgaagtagg
    62101 catcgtaaca gattggccat tatagcttcc tttaatccca aatagattgt aattaggggc
    62161 agaagccaaa gtactgttcc cccagccact ttcaacgatg gcttgagcaa tcatgactga
    62221 tgcatataag tcattggcac ttgccaaact ttgtgcagtt gaggcaatac tatcaatgaa
    62281 cgcttgttga gaattagcag atgagcgagc ataaaaagca tagccagtat agttttcgaa
    62341 ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc ccatctgtca attggttcgt
    62401 gatttcttct gcattctctg gattgttctg agcttctgct gatgtactgt cttcggtttg
    62461 tgtttgttct gttgtttcag ttgttggttc ttcggttgaa attgtatttt ctgagacagt
    62521 agctgactgc aaagtacttt ctgtatcgtt ttcaattcct gtttctgaag aactcgatgt
    62581 ctgagttgcg acagtttctg tcgattcttc agctagcact gttgtgttgg caactgctag
    62641 atttcctaaa agaataacgc taaataataa attctttgct ttcatctttt ttctcctttg
    62701 aattatgaaa cttttcctaa ataacagtgt aactaaaaaa gta
//
LOCUS       DAZKOB010000006        61090 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000006 DAZKOB010000000
VERSION     DAZKOB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.006"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1424)
                     /gene="sufB"
                     /locus_tag="ACG8M6_000386"
     CDS             complement(33..1424)
                     /gene="sufB"
                     /locus_tag="ACG8M6_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0456085.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(1429..1899)
                     /gene="sufU"
                     /locus_tag="ACG8M6_000387"
     CDS             complement(1429..1899)
                     /gene="sufU"
                     /locus_tag="ACG8M6_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0456086.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(1886..3121)
                     /locus_tag="ACG8M6_000388"
     CDS             complement(1886..3121)
                     /locus_tag="ACG8M6_000388"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0456087.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(3118..4404)
                     /gene="sufD"
                     /locus_tag="ACG8M6_000389"
     CDS             complement(3118..4404)
                     /gene="sufD"
                     /locus_tag="ACG8M6_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0456088.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(4417..5190)
                     /gene="sufC"
                     /locus_tag="ACG8M6_000390"
     CDS             complement(4417..5190)
                     /gene="sufC"
                     /locus_tag="ACG8M6_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0456089.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(5387..6235)
                     /locus_tag="ACG8M6_000391"
     CDS             complement(5387..6235)
                     /locus_tag="ACG8M6_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0456090.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(6259..6945)
                     /locus_tag="ACG8M6_000392"
     CDS             complement(6259..6945)
                     /locus_tag="ACG8M6_000392"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0456091.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(6938..7972)
                     /locus_tag="ACG8M6_000393"
     CDS             complement(6938..7972)
                     /locus_tag="ACG8M6_000393"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456092.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(8035..8291)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8367..8705)
                     /locus_tag="ACG8M6_000394"
     CDS             complement(8367..8705)
                     /locus_tag="ACG8M6_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0456093.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(8707..9063)
                     /locus_tag="ACG8M6_000395"
     CDS             complement(8707..9063)
                     /locus_tag="ACG8M6_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0456094.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(9069..10256)
                     /locus_tag="ACG8M6_000396"
     CDS             complement(9069..10256)
                     /locus_tag="ACG8M6_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0456095.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(10447..11292)
                     /locus_tag="ACG8M6_000397"
     CDS             complement(10447..11292)
                     /locus_tag="ACG8M6_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0456096.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            11526..12491
                     /locus_tag="ACG8M6_000398"
     CDS             11526..12491
                     /locus_tag="ACG8M6_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0456097.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            12868..13911
                     /locus_tag="ACG8M6_000399"
     CDS             12868..13911
                     /locus_tag="ACG8M6_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0456098.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(13973..14437)
                     /locus_tag="ACG8M6_000400"
     CDS             complement(13973..14437)
                     /locus_tag="ACG8M6_000400"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0456099.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            14683..15630
                     /locus_tag="ACG8M6_000401"
     CDS             14683..15630
                     /locus_tag="ACG8M6_000401"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0456100.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(15841..17913)
                     /locus_tag="ACG8M6_000402"
     CDS             complement(15841..17913)
                     /locus_tag="ACG8M6_000402"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0456101.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17846..18223)
                     /locus_tag="ACG8M6_000403"
     CDS             complement(17846..18223)
                     /locus_tag="ACG8M6_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0456102.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(18352..18912)
                     /locus_tag="ACG8M6_000404"
     CDS             complement(18352..18912)
                     /locus_tag="ACG8M6_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0456103.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            19062..20354
                     /locus_tag="ACG8M6_000405"
     CDS             19062..20354
                     /locus_tag="ACG8M6_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0456104.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(20414..21532)
                     /locus_tag="ACG8M6_000406"
     CDS             complement(20414..21532)
                     /locus_tag="ACG8M6_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0456105.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(21546..22967)
                     /gene="arcD"
                     /locus_tag="ACG8M6_000407"
     CDS             complement(21546..22967)
                     /gene="arcD"
                     /locus_tag="ACG8M6_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0456106.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(23213..24334)
                     /gene="mnmA"
                     /locus_tag="ACG8M6_000408"
     CDS             complement(23213..24334)
                     /gene="mnmA"
                     /locus_tag="ACG8M6_000408"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0456107.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            24738..25337
                     /locus_tag="ACG8M6_000409"
     CDS             24738..25337
                     /locus_tag="ACG8M6_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0456108.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(25475..26761)
                     /gene="efmA"
                     /locus_tag="ACG8M6_000410"
     CDS             complement(25475..26761)
                     /gene="efmA"
                     /locus_tag="ACG8M6_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0456109.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(27032..27376)
                     /locus_tag="ACG8M6_000411"
     CDS             complement(27032..27376)
                     /locus_tag="ACG8M6_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0456110.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(27513..28658)
                     /locus_tag="ACG8M6_000412"
     CDS             complement(27513..28658)
                     /locus_tag="ACG8M6_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0456111.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(28838..29812)
                     /locus_tag="ACG8M6_000413"
     CDS             complement(28838..29812)
                     /locus_tag="ACG8M6_000413"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0456112.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(29892..30767)
                     /locus_tag="ACG8M6_000414"
     CDS             complement(29892..30767)
                     /locus_tag="ACG8M6_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0456113.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            30897..32804
                     /locus_tag="ACG8M6_000415"
     CDS             30897..32804
                     /locus_tag="ACG8M6_000415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0456114.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(33345..36623)
                     /locus_tag="ACG8M6_000416"
     CDS             complement(33345..36623)
                     /locus_tag="ACG8M6_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0456115.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(36894..37439)
                     /locus_tag="ACG8M6_000417"
     CDS             complement(36894..37439)
                     /locus_tag="ACG8M6_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0456116.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(37451..38143)
                     /locus_tag="ACG8M6_000418"
     CDS             complement(37451..38143)
                     /locus_tag="ACG8M6_000418"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0456117.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(38162..38446)
                     /gene="macP"
                     /locus_tag="ACG8M6_000419"
     CDS             complement(38162..38446)
                     /gene="macP"
                     /locus_tag="ACG8M6_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0456118.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(38459..39022)
                     /locus_tag="ACG8M6_000420"
     CDS             complement(38459..39022)
                     /locus_tag="ACG8M6_000420"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0456119.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            39250..39912
                     /locus_tag="ACG8M6_000421"
     CDS             39250..39912
                     /locus_tag="ACG8M6_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0456120.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            40036..41232
                     /locus_tag="ACG8M6_000422"
     CDS             40036..41232
                     /locus_tag="ACG8M6_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0456121.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(41333..42709)
                     /locus_tag="ACG8M6_000423"
     CDS             complement(41333..42709)
                     /locus_tag="ACG8M6_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0456122.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(42746..43612)
                     /locus_tag="ACG8M6_000424"
     CDS             complement(42746..43612)
                     /locus_tag="ACG8M6_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0456123.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(43701..44726)
                     /locus_tag="ACG8M6_000425"
     CDS             complement(43701..44726)
                     /locus_tag="ACG8M6_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0456124.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(44744..45418)
                     /locus_tag="ACG8M6_000426"
     CDS             complement(44744..45418)
                     /locus_tag="ACG8M6_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0456125.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(45602..46495)
                     /locus_tag="ACG8M6_000427"
     CDS             complement(45602..46495)
                     /locus_tag="ACG8M6_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0456126.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(46569..48266)
                     /locus_tag="ACG8M6_000428"
     CDS             complement(46569..48266)
                     /locus_tag="ACG8M6_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0456127.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(48259..49572)
                     /locus_tag="ACG8M6_000429"
     CDS             complement(48259..49572)
                     /locus_tag="ACG8M6_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0456128.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(49572..53513)
                     /locus_tag="ACG8M6_000430"
     CDS             complement(49572..53513)
                     /locus_tag="ACG8M6_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0456129.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(53622..54080)
                     /locus_tag="ACG8M6_000431"
     CDS             complement(53622..54080)
                     /locus_tag="ACG8M6_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0456130.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(54378..54896)
                     /locus_tag="ACG8M6_000432"
     CDS             complement(54378..54896)
                     /locus_tag="ACG8M6_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0456131.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(54898..55514)
                     /locus_tag="ACG8M6_000433"
                     /pseudo
     CDS             complement(54898..55514)
                     /locus_tag="ACG8M6_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(55511..56299)
                     /locus_tag="ACG8M6_000434"
     CDS             complement(55511..56299)
                     /locus_tag="ACG8M6_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456132.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            complement(56361..57272)
                     /locus_tag="ACG8M6_000435"
     CDS             complement(56361..57272)
                     /locus_tag="ACG8M6_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456133.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(57313..58647)
                     /locus_tag="ACG8M6_000436"
     CDS             complement(57313..58647)
                     /locus_tag="ACG8M6_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0456134.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            58855..59685
                     /locus_tag="ACG8M6_000437"
     CDS             58855..59685
                     /locus_tag="ACG8M6_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0456135.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(59929..60178)
                     /locus_tag="ACG8M6_000438"
                     /pseudo
     CDS             complement(59929..60178)
                     /locus_tag="ACG8M6_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(60369..60563)
                     /locus_tag="ACG8M6_000439"
     CDS             complement(60369..60563)
                     /locus_tag="ACG8M6_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456136.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        17241 a        13151 c        11000 g        19698 t
ORIGIN      
        1 atatatcaag ggttctagct cctataacag gtttacccaa cactgccttc catctcataa
       61 ctgatcaatc gattgagttc gactgcatat tccattggaa gttctttggt gaatggttca
      121 acaaagccca tgacgatcat ttcagttgct tcagattcgg tcaggcctcg gctcatcaga
      181 tagtagagtt gttcttctga gatttttgag actttggctt cgtgttctaa agagacttgg
      241 ctgttgtgga tctcattgaa tgggatcgta tcggatttcg accattcgtc catgatgatc
      301 gtgtcacatt cgatatgaga gatcgaaccc gcactgtttt tggcaaaggt tacttgtcct
      361 cgatagttta cttctccgcc gtcttttgaa attgatttag agacgataga gctagatgta
      421 tttggtgcat tgtggatcat ttttgcaccc gtatcttgga tttggtttgc tcctgcaaag
      481 gcgatggaga gcatggtgcc tcgtgcacct tttccatcta agtaaacact tgggtatttc
      541 atcgttgttt ttgcacctag gtttccgtcg atccattcaa cggttgcgcc ttcgtatgct
      601 tttgctcgtt tggttactaa gttgtacaca ttgtctgacc agttttggat cgttgtgtaa
      661 cggcaatacg catcttttct tgtaaagatt tctacgatgg cggcatgcag gctgttactt
      721 gaataagtag gtgccgtaca accttcgaca tagtggatgc ttgctccttc gtcaacaatg
      781 atcagcgtac gttcgaattg ccccgtgttt tccgcattga tccggaagta cgtttgtaat
      841 ggaacatcga ctttgacgcc ttttggcacg taaataaagg tcccacctga ccatacggct
      901 gaattcagcg ctgctaattt gttgtcggtt ggcggtacaa gttttgaaaa gtactctttg
      961 aataattcag ggtattcttt caaagcagag tcagtgtcag taaagacgat tcctaacttt
     1021 tcgaattcat ctttcatatt atggtacacg acttctgatt catattgtgc ggaagcaccg
     1081 gctagataag ctcgttcagc ttctggtata ccaatctttt caaatgtatc tttgattttt
     1141 tctggtacgt cttcccagtc acgagcgggt ttgtcacttg gtttttggta atatttgatg
     1201 gcatcgaaat cgatgtctga aaggtcgggg ccccatgttt gcattggcat tttgttaaat
     1261 gtttctagag atttcagacg gaattcaagc atccattctg gttcgccttt gattcttgac
     1321 atctcacgga tcacttcttc tgtcaagcct tctcctgtac tgaaaaccgg ttcgacatca
     1381 tcgtgaaacc cgaatttata ttcttctaat tctggtacgc ccatgttctc actcctcttc
     1441 cttgtggtaa tgatggaaag ctgcggtacc atcgttttcc attgcttttc ctagtgcttt
     1501 ccaggcaagt gtcgcacatt tgatccgagc gggaaattta gctacaccac caagcatagc
     1561 agcatcgcct aattggtcgg gatctttgac ttcttttcct tggacaagct gtagaaagtc
     1621 tacagttaaa tgttctgctt cttctattga ttttccgatg acggcgtctg tcatcatcga
     1681 ggcacttgcc gtactgatgg agcagccact gccgtcaaag gcgatatttt tgatgattcc
     1741 gtcttttaca tcgacttgca attcgatcac atctccacat gtggggttgt tcaattcgat
     1801 cttgcttgtc gagtcagtca agcttccacg atgatgcgga tgggaagaat gatccaagat
     1861 cacttgacgg tataaattgt ctaatttaga tagtcccatg ttggaaaaac tcctttgttg
     1921 ccatgattgc ttcaactaaa cggtcggcgt cttgcgttgt gttgtagaaa taaaagctcg
     1981 cgcgagcagt cgctgatact tcaaggtact tcaatagcgg ttgcgcacaa tgatgacctg
     2041 ctctgacggc taccccttcc atgtctaaag ctgtcgcgac atcatgggga tgtaacccat
     2101 ccaaattgaa tgcaacgact cctgtccgaa aattcgggtc ttgtggacca taaacagtca
     2161 atccatcaat tgcttgtaat tttggcaaca cataagcggc tagttcttct tcatatcgat
     2221 ggatattgtc cataccgagt ttttgcaaat aatcgattgc atggcctaat gcgatagcgc
     2281 cggcgatatt tggtgtccct gcttcgaatt tccaaggtag ttctttccat gtactgtcgt
     2341 ataattcgac aaaatcgatc atttcgccac caaattcaac aggttccatt tcttctaacc
     2401 attttcgttt accatataaa acaccaatac ctgttggtcc gcacattttg tgtccgctga
     2461 aagcaaagaa atcacagtct agtgcttgga catcaatggc catgtgcggg gcagattgag
     2521 caccatctac tacgatgacc ccgccgtttt catggacaag ttcagctaat tcttgaacag
     2581 gattgaagac acctaagaca ttggatacat gagcaatgga gacgattttg gttttgtccg
     2641 taatcttttt cgctgcatcc tccatatcca ggaagccttc ttctgttagt tcgatatatt
     2701 tcaagacagc acctgttcgt tttgccaact gctgccacgg gataacatta gaatgatgtt
     2761 ccatgtaaga gagcactatc tcgtctcctg ctttgatata gcggtcgccg aaggttctgg
     2821 ctacccaatt caagcttgtc gtcgtaccac gagtaaacag cacttctgct gtttcttttg
     2881 catggatgaa cgcccgtact ttttctcttg cttgttcata atcatgggtc gcgcgttctg
     2941 ctaacgtatg gacaccgcga tgaacattgg cgttatcgtg ttcgtaatag tacgctaatg
     3001 cctggagtac ttgttttggc ttttgggtcg tcgctgcatt atccagataa actaaaggtt
     3061 catcattgac gtcttgaaat aagataggaa aatcattgcg tagcttttta gcatcgatca
     3121 tccattcaac ttcccttcga ttacttcgac taattctttc cgaacttgtt ctacagggat
     3181 tgctgttaag acagaaccga ggaatcctcg aatcactaag cgctctgctt cttctttatg
     3241 caatcctcta ctcatcagat agtacatttc ttctggatca actcgtccta cactggcagc
     3301 atgtccagca gttacttcgt tttcatcgat caaaagaata ggattcgcat caccacgtgc
     3361 tttatcagaa agcatcaaga cacgactttc ttgttgggca tctgcacctt ttgccccttt
     3421 caagatgtgg ccgataccat taaaggtcaa tgttcctttt tctcggatca ctccgtgttg
     3481 caaaatatgc ccgatcgtat gaggggcttt attggttact cgtgtatcga ttccttggat
     3541 ttgtctgccc gagctgattg ccacgatttt gacttctgcg tgtgcacctt ctcctgctaa
     3601 atctgagtca aagtcagcga tcacatgacc gtcattcatg acaccaattg cccagtcgac
     3661 agaagcatct cgtaaaatgt gcccacggcg attcatataa gaagtaatat tttctcctaa
     3721 ttgatctacg gctgaatatt tgatttttga accagcttta gcgatgactt ccacgatgat
     3781 attacctgat gattttgcta cttgttcttt ggttgtctgg aaacgttcca gataagaaaa
     3841 ttcactatgt tcatctgcca cgatcaacac atgcttgaag aagtgctcat cggatgcgcc
     3901 atcttgaatg aataaggact cgagtggttc atcgatcacg acatttttag gcacatacag
     3961 gaaaataccg ctgttcataa aagctacatg gagtgcagtc aattggtctt cgttcatgtt
     4021 tactgctttt ttcatataat attcttggac tagttctgga tattcgatca ttgcagtgaa
     4081 caagtcggta aaaatgactc ctttttctgc taattccaca ggcagttgtt caaaaatcgt
     4141 ccatgaacct tgttggacaa gcacaggatt gtctttcatt gcatcaaatg ccggaatcgt
     4201 gcctgtttca ctgatggttt gtgtttcttt cacatcaaat aacgaccagc gatggaattt
     4261 cacccgatcg atatgcggca atggcagttc atctgctttc gctagtgcgt cttgacggaa
     4321 ggttctcatc caatcaggtt cgcctttact taaagaaaaa tcatttaccg cgtcaagata
     4381 atcgagccag tttttttctt tcattcttgc tcccccttac gcttcttctt ctttatagtc
     4441 gattcctaat tctttactga taccagcgta gccttctgct tccagacgtt tagctaggtc
     4501 agcattccct gtcatgacta ctcggccttc catcatgatg tgaacgacat caggtgtgat
     4561 atagttcaat aatcgttgat agtgggtgat gatcaatgca ccgaaattgt ctccgcgcat
     4621 ttcatttact cctttagcaa cgactttcaa agcatcgata tccagacctg aatcgatttc
     4681 gtctaaaatt gcaaatgtag gttctaacat caacaattgc aggatctcat tccgtttctt
     4741 ttctccgcca gaaaaacctt cgttcagata acgttcagcc atttcttctg gcatatttag
     4801 taaagccatt ttttcatcta actttttcaa aaatgccata acagaaattt tgtcgttttc
     4861 atcacgtttc gcattgatcg ctgcacgcat gaattccgca ttcgtgatac ctggtatctc
     4921 acttggatat tgcatagcta aaaataagcc aagtcgcgca cgttcatcta cttccaagtc
     4981 taaaacattt ttcccgtcaa acaaaatttc gccttctgtt acttcatagt ttggattgcc
     5041 catgatagcg gcagataagg tagattttcc tgtgccgtta ggtcccatga tggcatggat
     5101 ttctccggtt ttcattgtta ggtttacgcc tttaaggatt tctttttcct cgatcgacac
     5161 atgtagattt tttatttcta agacagacat gcattatacc cccaaattct ctttcgttct
     5221 cactcatttt agactaattg agaatgaagc gcaatgacct tccttactaa aatcaaatat
     5281 cttttattta ttattaaaca attttgattt ctaaattaga ataggtataa aaaagatgag
     5341 aaataagcca ttacagactt atttctcatc ttttttgttc cataccttat tttgcatcag
     5401 cctttactgg tttaacagag ccgccccatt tgtctgtgat ccattcttgg acttctttgc
     5461 ggtgaagcac ttcaatcagt tttttgattt tgtcactgtc ttcatcacct ttacgagtcg
     5521 caataatatt cacgtaagga gagttgtcct tttcaagcaa tacggcatct tctaatggat
     5581 tcaaccctgc gccgtaagcg aagtttgcgt tgattgcaac taggtcacct tcatcatttt
     5641 ggtacgtaga agctaaaagt gatgggtcga tcgtatggtt gaacttcaag ttcttagggt
     5701 tttcttcaat atcatcgaat gttgctgttt ccagatctac tccatctttg actttgacta
     5761 agccagcatc ttgtagaatt gtgatgatgc gtccccagtc tgattcagaa tttgaagtaa
     5821 tgattgttgc accatctttt aattcggaaa catctttgac ttttttagag tacaagccca
     5881 ttggttcgat gtggacagca ccggcattga caaagtcata tcctttttct ttgatttctt
     5941 tgttcaaata aggaatatgc tggaagtaat tggcatcaat gtcgccttct tccaaagatt
     6001 ggttcggtaa tacatagtca tcgaatttga cgatttcaag atcgattcct tcatctttca
     6061 ataatggttt cacatgttcc aaaatctctg cgtgcggtgt cggtgaagcg ccgactttca
     6121 atgtcgtgtc gtctgcttta tttgctgaat ctttgctgtc gctgttgtta ccgcatgctg
     6181 ctaatcctac agtcagcaaa agcgctgctg caaatccaaa aattttcttt ttcatatcct
     6241 acatccccct atatcctatt atcttttgtc tgttttctta gttaagaaat caccgattcc
     6301 ttgaacgata aatacgatta gtaaaataat taaagtcgca acgaatgtca ctgttagatt
     6361 accacgctgg tagccttcct gccaagcaag acctccaagt ccacctgcgc cgatcgcgcc
     6421 ggccatcgca gtaaatccga tcatcgagat cgttgtaact gttaatccag aaatcaatgc
     6481 gggaatactt tcaggtatca agaccttcca gatgatttcc cagtaacttg ctcccattgc
     6541 atctgctgct tccagtacac cagggctgac ttcacgaaaa gcaatctcta ctagacgggc
     6601 ataaaatggt gctgcggata aaacaagagc tggaatggct gcttgcgcac ctagcatagt
     6661 gcctacgatt gctttagtaa atggaatgat caaaaccatc aggatcataa atggcgttga
     6721 ccggaaaata ttactgatga tcgatacgat tccataaatg attcgagcat acggtttgtt
     6781 tttgcgtcca atagagtaaa ggatcaatcc caaaactaat ccgatgatcg tcactaagac
     6841 cattgagatt aatgtcatat atagtgtatc aaggatagcc tgattcatcg tttccgggtt
     6901 gatttgttta aaatctagat atgtttctga aaagctttta tccacgatgg atcacctcaa
     6961 ttcctactcc ttgttctttg atgaacactt ctgcttgatc taattctttg gtttcaccag
     7021 tcatcaagac agtcaatgaa ccaaaggaac cttctttcac ttgtttgatc tttccataca
     7081 caacgttgac atcgatgtta tagcgtcgaa tcgcttggga aatgactggc tcgttggcat
     7141 ttctttcgct aaaggtcaaa gttgccaaac ggccgcttgg attttctttg atgagttcag
     7201 ctaatagttc tgtcgaatca gcttctggtt cgatgtcttg ttgtacaaag cgtttggtta
     7261 cttctttttt cggttgtctg aagacagtca atacgtctcc ttcttctacg actttcccca
     7321 actccatcac agcgactttg ttgcagattt tgcggatcac attcatttca tgtgtgatca
     7381 agacgatggt caaattcaat ttcttattga tcgctaacaa taagtctaat acttcttctg
     7441 ttgtttgcgg gtccaatgcg ctagtagcct catcacaaag caggatctca ggatcatttg
     7501 ccaatgctcg tgcgataccg acgcgttgtt tctgtccgcc ggaaagttgt gaaggatatg
     7561 cttgtccgcg tccttctaac ccgactaaac gcaacagttc ttctgcccgt tctttacgtt
     7621 tgctctttgg tacgccagct aattccagcg gcagctggat attttcaagt acagttcttg
     7681 accataacag gttgaaatgc tggaagatca taccgatttt ctttcggaat gtacgtaatt
     7741 ctttgttttt taattttgta atatcttgat cgtttacgat cacttcgccg ctcgtgggaa
     7801 gctcaagtcc attcaggaga cggactaatg tactttttcc tgcgccagaa tagccgacga
     7861 ttccataaat atcccctttt tcaacgttta gtgaaacgtc attgacggct tggatgtttc
     7921 cgtttttccc attgaatgtc tttttgacat tcgttagttg gattaatgac atgccattca
     7981 catctttctc ttataatttt tcaaacaaaa aagccttcgc cctcttgcct taagcaaaag
     8041 gacgaaagct tgaacttccg tgttaccacc ttgatttacc gttacttcgc aataacagcc
     8101 tcttccagta cttggactta gcttctttcc gtatactgtc gcgctgtaac gggcactccc
     8161 gtagtaagtt tgcttacgct cgcttactct gctctaagac catcttccat ctcattttcg
     8221 ttacccattt tcagcaaaca gggctctctt caaacgtccg tgacatgtac tcttcttttc
     8281 aaagctttct cgtttcatat aggattgctt atgattatag caactggaag aatcagtgtc
     8341 aacttttttc aagtgatttt ctgattttat aattcgtcaa attcttttgg atcaacatct
     8401 ttgaaactta agatccacgc attcatttca tctgaatcat tcaagatatt gatatcttca
     8461 ccgattttat cattaacaga agaaactgta cccgttagcg ggctggcaaa ttcattgacc
     8521 gctttttccg cttcaacttc gatcagaggt tcatcttttt ttagcgattg tcctgctttt
     8581 ggcagattgg caaacgtgat gtctccaagt tcttcttgcg cttcatttgt caaaccaaca
     8641 cagtattctt ttccattaaa taagatccaa aggttgtcct ttttcttcaa atgcttgcct
     8701 gccattttat atcagttctc cttcataaat tttttcattg aatccaattg caacaagctg
     8761 gtcctctcgt aagatcagtg gtcgcttgat cagcatacca tctgtcgata agacttggct
     8821 cgcttttgaa gccgtaaact cgcctacttg atctttcagt ccaagttccc gatatttcat
     8881 cccgcttgtg ttgaacaggc gacggattgg tacttgactc gtttctagcc aagtctcaat
     8941 cgttgaggcc ttcggtactt ctttgatcat atcaacggtt tgataactaa cgccatgctg
     9001 gtctaaccac gctttggctt ttttgcatgt tgaacatttc gggtaccagt aaaaggtgta
     9061 caattttctt acctccttga agatcttgca ggtgcttcat tttggtatct catcgataat
     9121 atcaacccga taccgatcat gttccctaaa atcgaggatc ctccctgact aatgaaaggc
     9181 aatgggatcc ctgttaaagg aagtaatccg atatttgctc caatattctc aaagacatgg
     9241 aacagcagca tcatgatcaa accgcttgcg atataggcgt aaaattcatt attcgtatcg
     9301 aaacacaccc gaatcatacg atagattaaa ataaaataca agaaaatgac gaatgcacca
     9361 ccgataaagc cgaagttttc accgataacg gtaaaaatca tatcggactc acgaacaggc
     9421 acatagatat tgcttacgtt gaatccttta ccaaacaagc caccggtacc aattgccaaa
     9481 agtccttggg ccggctgata actcattcct gacgtatcat ggaaagggtc cagccatgaa
     9541 tcaatacgtg caaactgata agatttgaat ccaactttat aaagcaagtc tcttccgcct
     9601 tcagtcgtaa ccaagaagat cgttcctgct ccaacgacta ctgccaagat tactacaggt
     9661 gcgatgatgc gccaagagat tccagacatc aggaaaatcc cgccaaagat cgcaagaaag
     9721 acaagcatcg ttccaaagtc atcttgggcc atgatcaatg ctaaaaccgg tattgtcacg
     9781 atcaagagct tcccaatcag ccaaaagtcc gatttcatcg tccgttcttt tacttgcgta
     9841 ttatgtttag ttacgatcag tgccatcatc aagatgtacg caatcttcat caactctgct
     9901 ggctggaaag taaacgcacc gaatcgaaac cagtttttag accctgttga agcagctaaa
     9961 ttctgatcat agaatttgag caagagtccc attaccacta accccaaacc ataaaggtaa
    10021 ggagtcagtt tccagatcca tttggaattc aaatgcatga tgacaacgat tgctgccgct
    10081 ccaactaaat accagacaac ttgcatccct acgcctttca agactgctgg attgttcgga
    10141 tcatggctaa gtgctacata taatgagagc agaccaatca aacataacag aaagactggt
    10201 aaaatcacgc cataatcaat acgattatcg ttcgaagctt ttaatttttt ttccatgttt
    10261 catccctttt cttaaaaacc atactgctga ttattatagc ggtccagtta gataatggaa
    10321 agtctttcca tagacatttg ggaaaaatta actttccgtt tgtgcagttg cttgagcaaa
    10381 tgcagtacaa aagcctactg cttatcttta gtgtatcata gaactttttt tgatacatgc
    10441 aattatctac cagctgagat tctcctcgct gtgagaagtt tctgtgcttt cattgtatcg
    10501 aaggatctct tcggcaatct ctagcagctg atcagaaacc agattggttc gttggatgaa
    10561 ataaaaatat cgatggaaat tcaacggagt cattggcaca ttttctggaa cagagcgctt
    10621 agacaaaatc gagcgaccga ttccttcctt cagcaacgca acaattacat cgttactttt
    10681 cacgatcatc ttcttatctt ggatattcat ctcttcaaaa taacgctgcg tgtaatgaaa
    10741 aacaccagag tttgcttctc ttacaagcca caatttgctt tttgggtccc cagcgatgac
    10801 tagctggtct tccatcaaag agtatctttg tacgccggaa gtctctaatg gcttttcgat
    10861 aaaaccaaaa tcggattcat gtttcgctac ttgttctaat gattcatgag aattcgtgat
    10921 ttcgatctca aaatgcactt taggaaacct tttgatcaga tgtttcatca actgaggcaa
    10981 aagatagaca gcaaaggtgt gggaagcaac gatccgacat acttcttctt cattttgttc
    11041 attgagcaat ttttgctgag tttcttccca ttcttctaac aaaaccagtg tccgttcata
    11101 taataagtcg gcttgcttag ttggaagcat ctcttttctt ccgttacgtt gaaaaagatc
    11161 gatattcaat tcccgctcca actgtttgat ttgattcgag actgctggtt gagataaaaa
    11221 cagtacttct gctgctctgg aaaaatttct tgtttcatat actgctcgaa aagtcgttaa
    11281 taacttaaac atggcaccac ttccataact ttttatgata attactataa aaactatttg
    11341 atttaaatat acaaaaaacc atctataata gaaatgtaca acaaaacagt tgctgatcca
    11401 actagtaact tagatgaaga caatcagctg tctgcttgct cgtaatcata cattcctatt
    11461 cattttacaa tgtattcgtt catcttggta ctaaaatcta ttaaataaaa aggagaatat
    11521 tatatatgaa gaactttttg aaaattattc cgatcccgat ctgcgggttg atccttgggt
    11581 tagcttccct tggaaatctg tttaaagact atcaccatgt tgctttaggc aatctaactg
    11641 gcggtatctc tatgattctt atgatattca tttttgttaa attgtttatc ttattcgaac
    11701 acacgaaaca aacgttgcat gatccaatca tcgcctccgt ttcaccaact tttacgatgt
    11761 cactcatggt tatttgtact tattttgttt catatgaaag tattgcacca attgtcaaat
    11821 tcatttggct gtgcgctgtt atcttccaag tgatccttgt cttttatttc aattatcacc
    11881 atgttatcaa agctgatctt tcaattgaag caatttatcc aagctggttt atcatttacg
    11941 ttggatttgg ggtcatcact gtaactgccg ggaacttctt cccacttatt ggaaaaattt
    12001 tcttttggat ttcattgatc tgctatgcgt tcttattgcc gatcatcatc aaacgtgtct
    12061 ttttcgtaag aaatatggct catccgacac gtccgctgat tacgatcgtt gcagcacctg
    12121 gttctctttg cctaacaggt tatcttaaaa actttgctga acctaatttt tatcttgtcg
    12181 tagcattgtt cgctttatct caagtattgt atttcgttgt gctgtctatg cttccacgtt
    12241 tgttcaagct tcgtttttat ccaagctatg cagcctttac attcccgctt gttatctctg
    12301 caacagctat atttactact gttcattact tcaacagttt aggtatctat agtagcgtat
    12361 tggatttctt aaaagttgcg gaatgtctgt ttgcaagtgt agttgtcttc tacgttcttg
    12421 ctcattacgt acgtttctta attaaagaac atagcaaaca tggaaaattt gtttctaaaa
    12481 aaatcgctta ataatcgatt caataggttg tgaaaaagct gttctgcatc aagtaataag
    12541 aacggccaaa caaaaacagc tgttcatgtt tttgtactgg ttgggcttat taccgcagat
    12601 gcaagctttt gaacaccgtt taccaagagg tcgtaaaaga agtggtcagc ttcaagaaat
    12661 aagaaggaat ttacgaaaat tgcttctcaa atttttgtga aatttcggct tatttccgta
    12721 gaagctactt ctggaacacc gtttattcga aacaagaggt cgtacaagac ttttgtcacg
    12781 acctcttgtt cttttgtcaa aatactggtt tagtgctact atgttcgagt atgcttttag
    12841 tatttattga cgaaaggaac cgatcaaatg aaatcattgt cacatccaat aaaatctgta
    12901 aaggattcag cggtccgcga aatcttaccc ggtttactgc tttcttttgc catcgctgcc
    12961 ttcagcaggc ttcttgctat ttatctgcca cagttaggtg gagcgactat tgctatttta
    13021 ttgggtattt tattaggaaa tacttttttt aaacagcctg ttctagcaat cggcacaaaa
    13081 tttgctgaaa gcaaactatt agaattctct gtttttttac taggtgcaac tgtcacagct
    13141 caaacgattg ctcaaattgg tgtaaaagga attgtttttg tgttactgca aatgtcagtc
    13201 actatcatcg gtgcttactt gattggtaaa aaactgcaat tcagcgatac ttcttctttg
    13261 atgatggctg gaggaaacgc tgtttgtggt tcttctgcta ttgcttcaat cgccccttcc
    13321 atccatgcga aagaggaaga aaaaggacag atcatcacac tagtcaatct actgggtact
    13381 gtgatgatgt tgactctccc ttttcttgga ttacagttgt ttggtggaaa tctcattgaa
    13441 aaaagtgcac ttttgggtgg tacactccaa tctgttggtc aagtagtcgc tggggcaagc
    13501 ttacttgaca attctgttgt cgaattcgcc atgctattta agattctacg tattatgatg
    13561 ctcgttgttg ttgtctatct gtttgaaaaa ttcatgcaca agaaacaacc agctctgttt
    13621 gaaacgccaa ctgatacgca gaaaaaacga aaattaccgc ttccttggta tgtattaggg
    13681 tttgtgatct tttgtttggt caacagcttg ttttctttac ctgtagttat tgaccaatcg
    13741 gcacatttta tcagcagctg gtttgagatc actgctttag cggcaattgg tctgcgttta
    13801 gactttgcaa aattcatgaa agaaggtaca cgcttcttat cttatggatt agctgtcggg
    13861 cttctccaaa cagtgatggc tttactattt atttggctga tcaaagttta gaatgaacat
    13921 gaaaaaacag ttgtgattct cgcatggaac aatgtccgaa tcacaactgt ttttaatttt
    13981 gcagatatcc ttctaacaac aacaattggc gtttgatttc aaccccatct attgcgtttc
    14041 ccatatatcc tgtcagtttt ccattttgcc cgatcactcg atgacaagga ataacgattg
    14101 gcaatggatt ggttcgattt gcctgcccaa tcgctcgcac agctttcgga ctatcaacag
    14161 caacggctac ttctttatac gttcggattt ctccataagg aatggtcgac aaagctttcc
    14221 agacattttg ctgaaaaggc gtacctctct ttatagataa aggaacatca aattcttttc
    14281 tttttcccgc aaaatattct tcaaactgtt cttgtgtgcg cgttaaaatt cgctgattcg
    14341 ctgattcgct ttctgaaatt ggctgaaaag atacagcagt caatccggat tcatctgcat
    14401 ctaaccataa aaagccgata ggtgtgtgta ttttcatttt gtttgctcct agtcaataga
    14461 ctggaacttc ctcctttttc tctatccaaa actacttgcc aactaataaa aattgttctt
    14521 aaaagtttga taaaccgagg aaacatggta aactaaatac gttagtttac aaaaagaata
    14581 cctgttcttt tacgtaaaat caaattttaa aggtgttatt taaggtttat taaattttga
    14641 atgtaaacca tatataaaca tacgattgaa aggatagaaa cgatgaaagt atcaattgtt
    14701 gtcccatgtt ttaatgaaga aaaaactgtt cctctttttt ttgatgaggt tgaaaaattc
    14761 aaaggtgccc atgagttcga atatttgttt attgacgacg gatcaaaaga cggtacgtta
    14821 gcagcaatca agaagctagc ctcagcttat ccttatgtcc gctttgtctc attttccaga
    14881 aattttggga aagaagcggc tctgtacgct ggattgcaag cttctacagg agatttagtg
    14941 accgtgatgg atgttgatct gcaagatccg cccgaactgc ttcctgagat gatcaaccgg
    15001 atcactactt ctgatatcga ttgtgtcggc acccggcgtt cgactcgtga aggcgagcct
    15061 gcgatacgca gtttctttgc gaaaaaattc taccagttga tcaataaaat cagcgataca
    15121 gaaatggtcg atggcgctcg tgatttccgc ttgatgactc gtcaaatggt ggatgccgtc
    15181 ctagaattaa cagaatacaa ccgattttca aaaggattat ttagctgggt cggatttaaa
    15241 acagaatata tttcttatga aaaccgcgaa cgtgtggctg gcgaaacatc ttggtccttc
    15301 tggaaattat tcaactactc gattgatggt atcgtgaact tctcggatgc accgttaacg
    15361 attgcttcat ttatcggcgc tttgtcttgt gtcggttctg gtattgcaat tttgttcatt
    15421 attttgcggg cattgttgtt tggtgatcct acatcaggat ggccttccat ggtctcgatc
    15481 ttcctgttca ttggtggaat ccaattgtta tgtcttggaa tcatcgggaa atatattggg
    15541 aaaatcttct tagaaacgaa acagcgtcct gtttatatca taagagaaac agaaaaagtg
    15601 aagccaaatg aattgaagcc tcaccaataa tatacgtcaa atgaagtaaa cattttttta
    15661 aatgaaaaag gactgtgaca aaagtcttgt cacagtcctt ttttccgaat aaatggtgtt
    15721 ccagaagtag cttcttcgga aataagccga aatttcacaa aaatttgaga agcaattttc
    15781 gtaaattcct tcttatttct tgaagctgac cacttctatc acaacctctt tcatatctta
    15841 ttacttcttc attgctgctt ttagtgctgc tgctaatggg ctttcttctg gttcttctgc
    15901 ttgtgcatat tttttcagca agcgtcgttc ttcatgtttg gtcattttct tcttctgctc
    15961 ttttttatca agcaattttt ctgtcgttcc atcgtattta caacggaaat aagcaccgtt
    16021 tttaccttcg atgatttcca ttttccggtg acattgcggg catcgatgat tagagacttt
    16081 tggatctttt cttctgcggt agttgcaatc tgaattggtg cagacataga ttttgccgtc
    16141 acgtgtgttt ttctcgcgca gattggcccc acattctggg catttctttt gggtaatcga
    16201 gaaatcttga tattcttgtt tgcttgattt gatctcattc accaagcgtt ttgtttcttt
    16261 ttcgattcca ttcaagaagg ttcctgcttg tttctttcct gcagcaatag cttcaaggtc
    16321 tttttcccag ctttctgtca aatctggtgt tactaaagag ggatttacca atactaataa
    16381 ttgcttacct ttaggagata cttgcagtcc ttgaggggta cgttccatca gttcagattt
    16441 gattagtttc tcgataatct ctgcacgcgt agctggggtt cccagactgt atttttccat
    16501 ttgaccaagc aagcttgctt ctgacaatgg tttaggcggt gcagatagtt cttttttgat
    16561 ggcgaaatcg ggattgattc tcattccttt ttcccatttg actgtttccg tctcagtttc
    16621 agtttcgcct ttccatcctg cttggacgac acgattttgc cgaaaaatga atcgttcttt
    16681 accaaattca accgtaactt ttgtttggct gactttatgg ggttgagcaa acagtcctaa
    16741 aaaacgagag acaatcattt gatagatttt tcgctcttca ttacttaatt tttcataacg
    16801 tgctctgttt tcagttggca aaagtgcatg gtgatcggtc actttactgt cttggaatac
    16861 ttttgtctgt tggacgatgg ctccgttttt caaatactgc ttgacctcag gagcaaagtc
    16921 gctgactgct tgcaatcgtt ctttcatcgt acttttcatg tcatttgtca gatatttgct
    16981 gtctgtacgc ggataagtaa cgatcttatg ggtctcatac aaactttgta caagacttaa
    17041 tgttttcttt gcagaatagc catagcgttg attggcttct ctttggatct ctgttaaatc
    17101 ataaggcaat ggcgcagatt ccgtttttaa tttttcttgg acttctgtga cagttccttt
    17161 gcttgtactg atcaattgaa caagtttttg tgcttcttct tgtgttttta aagcaaattg
    17221 atttttttga agcattttgg cttgttcttt tccagcttgc aacgtgatcg tgaaatacgt
    17281 ttgtggacgg aactgctcga ttttcttttc ttggtctctc actaatgcta gtgtcggtgt
    17341 ctgcacacgg cctgctgata aattatccgc gtacttcaca gttaaggctc tggtcacatt
    17401 caatcctacc agccagtccg cttttgcccg ggcaagagca gattcataca atgcatcata
    17461 ttctttcgca ggtcttagtt gcttgaatcc agttttgatt gctttatctg tttgagaaga
    17521 gatccataac cgcttcactg gtttattgaa atgcacatac tctaagatcc agcgagcaac
    17581 aagttcccct tctcttccgg catctgtcgc aatgactgct tctgagacat cttttcgctg
    17641 agccagctgc ttgattgctt ttaattgatg tcctgtttta ggaagtggtt tgattcctag
    17701 ttttttaggg atcattggca acgtgtccat ttgccagctc tgccattcct tattcagatc
    17761 ttccggcatt ttcaatccta ataaatgacc aagggcccaa gtaacgatca ccttgggtcc
    17821 ttcataataa tttttgtgct tttgattagc accaagcacc ttgcttaggt ctttcgcaac
    17881 gcttggtttt tccgctagaa ttagttgttt cataaaattc ctttctgctt tctactgtga
    17941 tgggctgatg atttttcaac aacgcaagtc ccttgtattg ctgtaaatca tcgtcacact
    18001 cttcacacca gcgctcatct ccgtcattcc aaaaggcgct gagaaagtta ctttttgcat
    18061 tgatatattc ataattttga tggatttcgt tccattgttt ttcgtacgct tgtcgtgctt
    18121 cttcgaaaga ctcgaatgtt tcttcttctt caatatcttc ttcccagtct tcaaaaaacc
    18181 accaaggctc attgtccccg aacatggtaa ttacttgata catactcgtg ctccttcttt
    18241 cagcttttct agactcatct tagtcacttt tcaagaaagt tgcaagaatt ttctcttata
    18301 aaagggtcat ttaatgaaca tcttaaaatt gttacaaatg aatctgattg tttaaggcaa
    18361 agtagcgata aagttattga tcatccaaaa atagctgtct tctggatcga ttgttggttc
    18421 ttttgtaaag tatcctaaaa aacgtaagga aagataaccg tggatcatac tacgaagttc
    18481 cctgcttcga tgaacttttt cttctttcgt taattgaaaa gcctcaaata tctgcaaaat
    18541 gatctgattc gtttcatgga ttccatctaa taaaacagct tcattggtat gcggtacact
    18601 gatcagtaac tcatacactg cttgatttga aaaagcaaag gatcgataag tttctgcata
    18661 gatctgcagt gcttcctgac cactttttcc cacaagagcc cgtcgcagct ggtcattaag
    18721 ttcctgcaga agtttcgctg ttagcgctgt tttgacatca cgaatatttt tgaaatggtt
    18781 atacagcgag ggagccttga tatccaagcg tttggctaaa tgctgaaagg ttatttgctg
    18841 aacatccatc tcttctgcta gttctcgaaa gcagtcgatg attttctctt gagacagatt
    18901 tctttttttc atttccatgt ttcctctcat aaaataaatc cttcactctt tcagtttacc
    18961 acgaaaaaga aagagaaaaa agagactttc attgataaac tacatattgt agtttataat
    19021 aaagagctgt agatgaaaat tttcagaaag aaggagtcta attggctgca agaataaagg
    19081 aaaaattatt taggaaaaga gaaacttggg aacaaaactt gtttgtctta tggtttggaa
    19141 catttatggc tggtatcgga tttagtttag tcatgccttt tatgtcactt tacatagata
    19201 cgttaggtaa ttatagtacg tcacaattga acttttggag cggattgact ttttcatcta
    19261 ctttcttggt gactacgctt atctctcctt ggtggggaaa attagccgac caaaaaggaa
    19321 gaaaattgat gctgttgcgt gcttcattag gaatggcaat cgtcatttct ttgatgggtt
    19381 gcgtgactag tgtgtatcaa ttagtcgcat tgcgtctgct tcaagggatt ttttctggtt
    19441 atatcagtaa tgccactgcc cttgtcgcaa ctgggacgcc aaaagaaaaa agtggtcaag
    19501 ttcttggaac cttagcaact ggttctgtta caggaacatt acttggaccg cttcttggcg
    19561 ggatctctgc ttcagcgttt ggctatcgcc ctaccttttt cattacaggg atcatcttat
    19621 ttttcgtctt cttgttgagt ctgtttttcg tccatgaaaa atttgtccca gtgaaaaaag
    19681 aaaacatggt ttctgccaaa cagattttca aagacttgaa atatccacac gtcgtcatcg
    19741 gtatgttcgt cacaacgatg atcatccaag catccaacaa ttccattagt ccaatcatca
    19801 gtctgtacat tcgccaattg ctgcatggac atgggaatgt tacgttgatc agcggaatca
    19861 ttgcttcgat acctgggatt gccactttga tagccgctcc aagatttggt cgcttaggtg
    19921 acaagattgg gagtgaaaga atacttgcga ttggtttggg ttttgcgatt ctggtttata
    19981 tcccaatggc attcgtcacg aatgtttggc aattagctgc cttacgtttt ttgattggta
    20041 tctctgatgc ttgtctgctc cctgctgtcc aagcattgat cacaaaatat tcgcctcatc
    20101 aagctgctgg acggatcttc agttataacc aatctttcca agcaactggt aacgtagtag
    20161 ggcctatgat cggctccagt gtttccagtg catttggtta tcgcggcgtc tttctttcga
    20221 cttctcttct tgttttggtc aacttcttgc ttgtacgaca caatacacaa gagatccatc
    20281 agcaagaaac tgcagaagaa aaagcttcag gagtctccat agcttcaggg cactctcctt
    20341 ctcatagaca ataagcgtaa atgaaaaaca ctgcttgaac agttgtcaca gactgtcctt
    20401 gcagtgtttt tgtttaggcc tgttttttca agaactcaga taagaggaat aatccctttt
    20461 ccaaggtttc ttttttacaa cagtaaccta gtcgggcatg cttcggtaaa tcaaaggcac
    20521 ttcctggtac taatagtact cctgtttctt ctaataaatt gatacaaaat gtctggtcat
    20581 cttcttcgat ggctaattta atgaaagaag tagagacgta attgggagcg acaagttcaa
    20641 ctttcggttc attggctacc cattgtttta aaatcacaag gttctctgta atgatctttt
    20701 tgtttcgcgc caatattttt tctttattct tcaatgcatg tacagcaaga tcatctgaca
    20761 ggactccgcc gcaaatcatc gtgtagtctc gatactttcg aaaaacttct gccaactcag
    20821 gtccagtagc tgtccagcca atacgaatac ctggaatgga gtaggttttg gatagggaat
    20881 tggttgctat acctttttca tagacatcta cgatcgacaa gaattcacct ttatccgtta
    20941 aaggcgcata tacttcatct atcaatacat atgcatctac tgtacgagca atttctgcga
    21001 ttttttctaa agttttccga tctagcaatg tgcctgtagg attgtttgca ctattcaaac
    21061 agatgatttt tgtttcaggt ttgactaagg cttctagctg ttctaaatca aactgccaat
    21121 cttcttcttc cttcaaatga acaaagtcaa cagcggcacc taaagatttg gggatgtcat
    21181 ataattgctg ataagtagga agcatcgaaa caacatggtc tcctgcttcc actaacgcgt
    21241 atagagctaa aagattagca ccagtcgctc cattcgtttg taagatgttt tccggatcga
    21301 cggtttgata aagagaagcg acttcttgtt tgaaaacatc tgatccttcg atccagccat
    21361 agtccaatgg ttcgtttttg cgtttttcga aaaatttact tacgctagtc ccatctaagc
    21421 caacgagttc gtctaatgtc aatgcttcga tcgagctttg tgcaatatca tataccgctt
    21481 tcttttccca tttatttagc cattcttcta caccgaaatt tgcgattttc atgtttcttt
    21541 cccccttata tcttgatcca gcctacagca agcaacccga ttgaaagcag tgcggccaca
    21601 caaatcagcc caatcattac tttttctttt cccgtaattt gatgtttggt ttcacgacaa
    21661 gcgatccagt agaagcaaaa accaggaaga taggcaatac tacataaaag gacataagaa
    21721 aatcctgcta aaatgatgcc catcaattca aaaactacag caatcccacc gatcactact
    21781 tgttttttga ttccttcttt taaagaaaac tggacttgat agatcccgac aagtagataa
    21841 caaatcaaaa ttgccgctgt acataaagaa taggcaaatt gataggcata atcagtaact
    21901 aagaaagtca ataagaatag attagtcaag attgcagtaa caactaacga ataagttggg
    21961 gcattttttt tattcgtttt tccccaacct ttcggcaaca gaccgtctcg agccatcaac
    22021 atcgttgttt ctgctgggag catcgtccaa gacaaccaag aaccaatgat cgaaataatc
    22081 aagccgatat tgatcagcgc tgctccccaa tttcccacga ttccttttaa aatatttgcc
    22141 atagcaggtt gtgaaatagt tgctaactct tcttgagata agacgccgta tggcaagatc
    22201 gaagcaagga tatagataaa caataaacaa agcagaccaa tgatcgatgc ttgctgggct
    22261 tctgaacgtt tcttcgcgcg gttggctaaa acacttgcgc cttcgatccc tacaaagacc
    22321 cacatcataa ccatcaaaca acctttaatc tgcaaccaga tcgttccttg ctgccctgtt
    22381 ccattcacta gattatcaga tacgttaccc caaaaatcag cagtgaatat attcacttta
    22441 aatgcgataa atacaaatat taaaaatagg aataatggca ctaatttaca gatcgtcacg
    22501 attgtattga tgacgctcgc tccttcaact ccattgttta ctaataaagt cagaccccaa
    22561 ataaaaatac ttgcaaataa aatagaagga acattttgtc cacctttgaa tatcgggaaa
    22621 aattctccca tggcgctcat cagcatcgtt gcaaaagcaa catttcccag ccacgcagac
    22681 aaccagtaac cccaaccact gatgaagcca cctaatttac caaatgaagc ttccgcgtaa
    22741 ccgaaaatac caccatctag atctggacgc ttttctccta aatgattcag cgataatacc
    22801 aaagctaaaa taccaatccc aacgattatc catgaaagga tcgctggccc tggagctgca
    22861 gaactagcca gatcacttgt gatcccaaac acaccgcttc cgattgacga actgacaacc
    22921 agagcaacca gtccccattt gctaataccc gtcgatttct tttccatttc catcaccctc
    22981 acttataaaa ttgaacaaat cacttgcaaa agattccctt ctattttgca actgctcttg
    23041 cttaacaaaa agcaacttta ttcatattat tggatattat gaacttttta cacaagtatt
    23101 tcaagtataa aatgaataaa aattaatttt attgcaaaaa aacagctatg ctatccaaaa
    23161 gaaaaggtgt ttcttttgtt agccatagct gttttgattc atttaatatc gcttaaacgt
    23221 attgtaaaac ttttgtttct tgatatgcgt ggtcgatcaa accaccaccc agacattcca
    23281 ttccatcata aaatacaact gcttgtccag gagtgattgc acgtacaggt tcgtcgaata
    23341 cgacttctgc acgattatca tccaataagc gaactgtcac aggaacgtct tgctgacgat
    23401 aacggaattt cgctgtacac ttgaattctt ttggcattgg ttcattcgtc gtaaagtgga
    23461 tttcacttgc atccaagctt gtggcataca atgcaggatg atggaaccct tgccccacat
    23521 ataatgtgtt tgtcgctaga tcttttccta caacaaacca gggttcttgc gaatctcctc
    23581 cgccaccgat ccctagtcct tgacgttgac caatcgtata atacatcaag ccagcatgtt
    23641 gtcctttgac ttcaccatca agagtgacca tatttccttt ttttgctggc agatagttgc
    23701 tcaaaaattg tttgaagttc ttttcaccaa taaagcagat tcccgttgaa tcttttttct
    23761 tagcagtcgc aaggcctgcg cgttcagcaa ttgcccgtac ttctgatttt tccatgccgc
    23821 ctagagggaa catcgttttt gaaagctgtt cttgtgataa ttggctaagg aagtaagttt
    23881 gatctttatt attgtctact ccacgaagca tgtggaccac gccgttttca tcgcgagtga
    23941 cttgcgcata atgtccagta gctacataat cggcgccaag atccattgca taatccaaga
    24001 atgctttgaa tttgatttct ttgttgcaca tcacgtctgg gttaggtgta cgtcctgcgc
    24061 ggtattcggc taggaaatat tcaaatacac gatcccaata ttctttttca aaattcacag
    24121 agtaataagg aatcccgatt tgagcagcta ctttcgctac atccttataa tcttccgtgg
    24181 ccgtgcatac accgttttcg tccgtgtcat cccaattttt catgaatatt ccgatcacgt
    24241 cgtatccttg ttcctttaac aaaagtgctg ttacagatga gtcgacaccg ccgctcatcc
    24301 ctacgacaac gcgtgttttg ctgttgtctg tcatgatatc accatcattt caattagatt
    24361 ctgaaacatc tacgagttga aggtcgcaac ttttcagtat tttacattat acgtcatatt
    24421 tctgaaaatt tcatccctta attatcaagt cgtaaaaata ctgttctact ggacctattc
    24481 agtacacaaa catgatagtc aaccaaaaaa tcactcatct tttgagcatt cgatataaaa
    24541 aattggcata ctcataaaaa gggccagcct ttcggcaata aggaataatt ttataaattg
    24601 tgaaaaacta tttttcaaaa ttgcttctta tcgcttaaag ctgcacgtct catttacaac
    24661 cttttctttt tagatgttat gagttgctca gataagtgat tttgtttact ctaagaaaaa
    24721 agggaggcaa aagatacatg aattacttta tcagtgatat gcactttttc catgaacgct
    24781 tattaggaaa aaacgacttt gctccgcggc tctttgcaaa tgtggatgaa atgaaccaac
    24841 agttgatcac tagctggaat gcagtaataa aagaaaccga ccatgtttat catctaggcg
    24901 accttgctat gcatccgcaa tatgaaaaag caaatgcaga aattttggaa ctggtcactc
    24961 aactgcaagg aacaatccac ttgatcaaag ggaaccatga cagtcgcgct tttttccgtt
    25021 atttagaaaa aaatgatcct aggcttactg acggaagtcc caaattttat ttttatgatg
    25081 taggtgccat cgttaaattc gaccatcaac aatattactt gactcattat ccgatgttgc
    25141 tcgggcctaa cgacactatc cgcaatcttc atggacatat ccatcattac agcgtaccta
    25201 tcgagaacga tgtcaatgta ggcgtagacg caccggaaag agaactctta aaggaaaaac
    25261 taccatttgg cactccgtta tctgagcaac aaatcaaaga aatctatgaa gcaaagagga
    25321 agtacgatag gtcgtgaaaa aactgcccta tatcaagtat taaacaaata aagataatta
    25381 ctcatatttt tcatttgagt aggcttattg ccacaggaac attaggatca gaaatggagt
    25441 tgtaacaaga cttctgtcac aactccattc catactattc gtctttttgc tcatggtgtt
    25501 cttttaccag ttttttctgt aattctttat cataatttcg ggcagctgac gttaaaaata
    25561 aaacgattcc gcataataaa atcccgattc cggaaaacaa gaacattttt tctaccccta
    25621 aacgatcagc taatggtcct gcaaataata atccaattgg acctgccagg ttcatcaaag
    25681 agttgaagtt ccctaagaca cgacctaaaa cattcgattc ataactctgc tggatcattg
    25741 ccatcagcag tgtattgaaa tatggcgttg cacaacctgc tcctgcattc aagataagaa
    25801 agtacaagaa cccttgacta tcgtttggaa ccaatccgct tgcaccgatc gtcaggccca
    25861 taatgatgta ggctataata ataggtttca ttcggtcttt ccatgttcct ataaaactga
    25921 tcagtgcacc gcctaagagc attccgactg cgtagactac ttccactgct cccgcctgtc
    25981 caacggaacc accaaagtaa tctagcgtca tcaatggata cagactgatt gctggcatga
    26041 acaacagcat cacaaatgca ccattgatcg ttatgtacca taacccttta ttatccatca
    26101 attgctgcag cccaaatttt gcatctttaa aaatagtaag cggaacttcc aatatttcag
    26161 gagaaacttt tgggatcttc acaaaaatca ataagccgac accaaaaact gcccccaata
    26221 catctaataa aataaggtaa ttgacaggaa cgactgaaaa caaagccgca cctagagctg
    26281 gcgcaatgat gaaattcgct gattggacca ttcctaactg tccattcgtc cttgttatgt
    26341 gagaagatgg aacgattgtc ggcataatcg actggatcgt tggcatttgg aatgtttgtg
    26401 cgaccgaacg cataaacaaa gacacaaaaa ctagccaaat cgggaaggat tctgaaatcg
    26461 ttccgactac tgctaaaata agcgcgaaaa tcgctacaat aatatccgtg acaatcaaaa
    26521 gagctttttt atcccagcga tccactagcg gtcctacaaa tgggcttagt aaaaccatgg
    26581 gaatcattcc tagtaaagta gcaaaactca aaattgtcgc cgaaccggtt tctcttgtca
    26641 aataccagat gatcgcatat tggacgacca tactagtgat tcctgacaaa aactgtcccg
    26701 ataaaaagaa caaataattt ctcttccaat tcgttaaaac gaccgactgt tcattttcca
    26761 taaacatccc tcttttcttt ttcattttgt atagcgtttg cacaacaatc attatacggg
    26821 attctttttt caaaaaatac ctttttccct aaataacaat cgtttcaatt taaatttctt
    26881 tacttagaaa caataaatca gcaaaatatt aaaaataaca acatgtgtta aatttaaaaa
    26941 taaaaaagac aactaatttg cgtcgtcctt acaaagttag tcatctaaca ataggccgtt
    27001 tcaaaactag ttgtcttttt atatccacag atcattcgac tttttcgaat aacccacggc
    27061 taatcatccc atccatcaga aaataaaatt tatcttggct cattttctgt tttggatctt
    27121 tgaaaatatt gactgctttc aatccatttg ctgtagtgat cgacattttc aatgtttgaa
    27181 tatcttttga tacagtgatt tttaatttga atccttcttt gctttgcggc gtggcatcta
    27241 atggtcggat caattgataa ttgcctccgt ttgtttctgt aaatccatta tcgcgcaatg
    27301 tatatctttt tgcatcagga tgcaaacgat agctaattgg tgcgcccagt accgttgctg
    27361 ttgtttcaaa tgccatgatt ttcacctcga taaaattagt ttctctttat tataaaagtt
    27421 tatcctgatt tttactaggt ttttcagtta agttccctaa aaaatgaagg cgttctctca
    27481 gacatctgtt catataagaa tgacttcact agttattttt tgacagcacg aactaattga
    27541 tcaaggaaat actggatgtc gtcttcggta tttcccaaac caaagctgat acggatggat
    27601 tcttttagta tcggtgtatc ttctccatac attgcttcta atacgtgtga aggctcgata
    27661 tttcctgctg tacaagctga accagtagaa atagcgaccc cattaagatc aagcttcatc
    27721 agtagcaaat catttgcttc gccaattaag cgaagattca acacatgagg gagtttattc
    27781 gttaaatcac catttacttg ataagggatt tcgtttgctt ctaatgtatc aagaatcaat
    27841 tgctggaatc ctttatattt gttgttacgt gcttcacgtt cttcaggtga cagcagttca
    27901 actgcttttg ccatacccca gatccctgct aaattttctg tacctgcacg ccgcttttct
    27961 tcctgctccc cgcctaaaag caatgcaggg atatttactc cgtctcgttt atataagaaa
    28021 ccgacgcctt ttggcccatt gattttatga gcagagatac ttaataaatc gattcctaat
    28081 tcttctggat ggatcgtttg gtttccgtac gcttgtaccg catctgtatg gaaataagct
    28141 ggatgatccg ctaaaagttc accgatttct ttgattggca tcaaatttcc gatttcattg
    28201 ttcccgtaca tcacagagac caagatcgta tcttctcgta acgctttttt gacctcttcg
    28261 atcgacagat tccctttttc atctacaggc agatatgtaa cgtcaaaccc ctttgtctct
    28321 aaatatttca tggtattaag aacagcagga tgttcgatag ctgtagtgat tagatgtctg
    28381 ccttcttttt gacgcgagaa cgctgtttct aagatagctg tattatcccc ttctgttccc
    28441 ccactgttaa agatgatctc gtgaggtttt gcttgcagac tgtctgcaat gatttgtctt
    28501 gctgtttcca atttttcatg cgcgtggcga ccaaatccat gtatgcttga aggatttcca
    28561 aacgtgtctt gcatgattgc cgtcatcgct tcaattactt gtggatgcat gggggtagtt
    28621 gccgcatgat ccagataaat agttttcact tcattcatcc cttccaaatt attgctaggc
    28681 acattctagc ataaaatagc gaacaaacca aagaagagct catttttgtc tagaggacgt
    28741 ttcatgggca tagaaaaaag gagctgtgac aaaagtcttg tcgcagctcc ttgaagctgc
    28801 tcacttctgt cacaacttct ttccctcttt tttttgttta ttcgaattct ttttcttcca
    28861 gacggaataa cggactcatc ggtgtatttt catggatgcg tttgaccgct tcacccatta
    28921 attctgaaca tgtaacgatt gttaagtttt ctggatgacg attttcttct gtgaaaacag
    28981 aatcagtgat acaaatatct ttgattggtg cattgtctaa aatacttttc gcatgatcgg
    29041 acaataatcc atgtgacgca caggcatata cttcttttgc gccgttttct tttaatacat
    29101 ttgctgctcg tgcaagcgtt gctcctgtgt tcaagatatc atctaccatg atacatgtct
    29161 ttccttgaac atcaccgatc acataaccac cctcagttga agcatcatct gcatgatcta
    29221 cgatggctaa tgtgctgttc aaatattctg aaagactacg tgcacgctga acaccgctgt
    29281 tttttggcga aacaactacg atgtcatctc ccgtcagacc taattgacgg taataatgag
    29341 caaacaacgg catcgtaaat aaattatcta caggaatatc aaagaatcct tgtacttgaa
    29401 ccgtgtgcag atctaaagtc agtactcgag ttgcccctgc ttcttcgatc aagttagcga
    29461 tcaatttcgc agtgatcggt tcatgaggtt tcgccgtgcg gtcttgccta gcatacccat
    29521 agtaaggcaa tacgacattg atcgttttcg cacttgctcg cttcattgca tcgatcatga
    29581 tcagtagttc catatagtaa tcattgacag gtgcattcgt tgattgaacg atatacacat
    29641 gatcccctcg aatactttct tcgatattga tggaaatctc gccatcacta aattgcttca
    29701 cgacgctttt acctaattct gttccgaaca ctttcgcgat tttttcagct aatggacggt
    29761 tgccattcaa actaaaaatc ttcaacgtgt catcctgata cttattactc atgtagtcct
    29821 ccccaaaact tctctattct tcgatagttt aacatatttt tccaagattt tctgttaatt
    29881 tcagcaaatt cttaaggaag tgaaactgtt ccgttactcc aactggcagg aatgacttta
    29941 tgataatcgc cccagttgtc gtgtactttg taataacgtt ctcctgtttc agtttcaaaa
    30001 taagcgtacg ctgtcaccca atgattgcca tatgtactgc caagtacttt caaaatacca
    30061 atcatcacag gtttgccttc acgtatacgt tttgtagctc gctgccatga acctagaaga
    30121 gttgctcgtg cacgctcatg attgccattc ttctttaaaa aactagaaat ccctgtacta
    30181 acctgaaaag gaatcgtagg aagtcctagt ggttggatct ggctacgcaa tgccttcgtc
    30241 aaaaccaacg aatcagccgt atttttcttg cgaatctcat ttggaagcat ccacccctta
    30301 cgatagtctt gataataggc aaggagaact gctgccgcgt aagtaccaca aagatagcct
    30361 gagggggtag cccatttctt atacctcaaa aaacgttctt ctggcaatcc cacccagcaa
    30421 tttgcgagtt cctttcctga ccttttttct tcgatcaaaa cgggaatatc ttcttttctg
    30481 ttgatccatc tagataactc gattggctct tcattgatca gcttccattt tttacaagaa
    30541 agaccctctt gataagtata ttttgctgga gaatattcta ccggatgtcc tgattgatcg
    30601 ataatcacat aactttgatc ttcaaaaacc agagctaaaa aaagcaattt actttttctt
    30661 gactgcagct gctgagtgct ggctaatttt ttaatttttt tatctacttt gaaatataaa
    30721 ggtttatcca ctacttgatt cagttgggat cgcctccttt ttttcattat aagtgaagga
    30781 ttgaaaaaaa caaatcagtt tgattgttcg aattttcaat tttttatgtt ctatttgtta
    30841 attccttcat tttgtaagat aatgggcaca cgaaacaaaa aaggagcgat cgatgaatga
    30901 acaaagaacg attgaaacaa gatttctatg aagcagtcaa tgaagaatgg caaaaaacag
    30961 caaaaatccc tgcagataaa ccagctacag gcggtttcca agatttagtt gacgggatcg
    31021 accaactttt gatgggagaa acagacgatc tgcttgccca tccagaaaaa gtgtcttcag
    31081 aaaaaatgaa acatttcctt gcatattatc aactggcaaa cgattatgac cgaagaaatg
    31141 agttgcatgc tcaaccgctt caacctataa tcgaaaaagt tgaatcattg cagtctttcg
    31201 acgaattgaa taagcaattt ccagaatggc tgttggatac attaccattg ccatttaatc
    31261 tggatgtaga tgcagatatg aagaatgcgc aaacgaacgc cttctttgct tatccgcctt
    31321 ctttattcct tccagataag acgtattatg catcagacca tcccaacggt gcacaactaa
    31381 taggcgtttt ctttgatatg gctgttcagt tattagagct tgccggcaaa gaaaaaagtc
    31441 aggcagaagc aatcacggaa caagcagtgc aatttgataa attgattgct cctcatgtaa
    31501 aaagtgcaga agaaaacgca gattacagca aaatgtataa tccgcaatct tttgatacat
    31561 ttattaaata cacagataaa ctggacttga aaaaacttgt cacgggtttg attgggacaa
    31621 ctccagataa agtgatcgta actgatccag tgtacttcga tcatttgaat gacatcctga
    31681 cagaagctca ttttccgtta ttcaaaagct ggatgatcat taaaacagta cgttctctta
    31741 gtggttatct atcagaagaa ttccgccaag tcagcggaat ctattctcgc gtattgtctg
    31801 gaacagacga agcaatgcct cagaaaaaag cagcgtatta ccttgcctct ggacaattcg
    31861 atcaagtagt tggcgattac tacggaaaaa aatattttgg cgaagctgct aaaaaagacg
    31921 tagaacaaat ggtgaaaaaa atgatcgccg tttataaaaa acgtctagaa gaaaatgact
    31981 ggcttgggga tgcaacaaaa gaaaaagccg tcataaaatt aaataactta ggtatccaag
    32041 taggctaccc cgataagatc ccagcattat tcgaccagtt caaaacagta cctgcagatc
    32101 agggtggtac tttgctttca aatactcttg ttttcagcaa actcacatta aaagatcgtt
    32161 tcagcaaatg gaacaaacca gtggatcgtt cagaatggga aatgagtgct gacacggtca
    32221 atgcttatta tcatccattt cggaatgtga tcgtctttcc ggcagcgatc cttcaagcac
    32281 cattttacag tctagaacaa tcaagctctg ctaattatgg tggaatcgga gctgttatcg
    32341 ctcatgagat ttcccatgca tttgataata acggcgcttt atttgacgaa tatggcaact
    32401 tgaacaactg gtggacagaa gaagatctta cacaatttca aaacaaagca caagcaatga
    32461 tcgaagaatt cgatggtctg ccttttgctg acggaaaagt aaacggaaaa ttaaccgttt
    32521 cggaaaatat tgctgatgca ggcggtctaa gctgtgcatt ggaagcagca aaaacggaat
    32581 cagatctttc tatcgaggaa ttctttgtca attgggcaac gatttggcgt acaaaagcaa
    32641 aaaaagaata ccaacaactt ttactgcaaa tcgatgtcca tgcccctgct aaactccgag
    32701 caaacgtgca accaaaaaac cttgacgaat tctatgaaac atttggtatc acggaacaag
    32761 acgctatgta tctagcgcca gaaaaaagag tgcgaatctg gtaagaggtt gtgccagaag
    32821 tggtcagctt caagaaataa gaaggaattt gggaaaatcg cttctcaaat ttttgtgaca
    32881 tttcggctta tttttcgaag aagttacttc tggaatacca tgtattagag gttgtgaaaa
    32941 agctgcttag tttcaagtag taagaaaaat tttgtgaaaa tggctttcca catttttgca
    33001 taaatttgac ttactaccga gaaactggct tttgaacacc gtttatcgag aggttgtgaa
    33061 atcagcgtcc tgacctgagt attagagaaa aaaacggaaa aatagttcct catattttgc
    33121 cgtttgttga gctaataccg agggtcagct gattgaacac cgtttagtgg agagattgtg
    33181 aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaagcagcaa ggagaacctc
    33241 atctataaaa agatagaagg aagtgagaca tctattggtc tcatttcctt ctatttttac
    33301 tttcctcagt caattgtttt acatcataaa aggagaaatt ccacttattt ttttcgctgt
    33361 ttcatgacat aaacaactac accgattaac aaaattcctc cagctaatgc tagcaacggg
    33421 ttattaaatt ctcccgtttt tgggagtcct tgagtgccat ttttttcact ttcttttgct
    33481 gtcctatccg gatgagaagg ttttccattt ggttttccat ttggtgattc atttcctgga
    33541 ttttctcttt ctggtttgtc atttcccggg ggcacatcgg cagtttctcg cacagtgatc
    33601 gctttttgct gggtaacaga ttctgtatga gcagaccatt tttccccatc aaatatttcc
    33661 gaacgataag tgacttctag cgtatattct ccttttgaag gtgctgaaaa aatagcggta
    33721 tgattagaaa attgagcact tgactgtgtt tcaacgattt ggatcttttc tgggaaataa
    33781 cgcatatctt caaagacagg cgttccttga tttggtgtga aagttgacac aaaaactgta
    33841 aaaggcttca gttcaatgat atttccttct gcattctccg ccacaccatt tgcttcttgt
    33901 ataataatcg gattattttg ttcatttggt tttgtctgat ttggttcttg tacgattgca
    33961 tactctggat attccgaaga cggtgaggtt tctccgtcaa aaatagcacc tacagataat
    34021 ggaagaccat ccactctttc cttttctgtt tgacgattcc ccggtttaat ctgcatcagt
    34081 ccatgaacat ggatacttcc attttcatag cgcgtaacag gctgctgacc ttctttcgaa
    34141 catgtattta gagactcgat ccaatctttt gatatggtat gtccaagagt attttctgat
    34201 tgtttctctc gttcattcca atagtagttc aacgagtggt agatttgatc ttcgttttga
    34261 ttcaatgggc ggatctgctc tctcatctca agattctctg ttcggaaaga taagatacca
    34321 gaagcatggt agtctgtgtt tcctgctgaa gaggtataaa gagccacatt atatgaaccg
    34381 ttattaaaac ttgtactgct attgacgata atatctgttc cgctattgga atcgattcct
    34441 ttcgcaagat tttcgaaaga caaactattg atcaattcat gagggcccgt taaagaagaa
    34501 ccgcccattt tgaatccatt accgttacct tctccctcaa ttcctggtac ccagccgttt
    34561 cgataagcaa ctgagttttt gatgattact tttccgatcg aacctgtttc atttttagca
    34621 aaaaggtccc aaccgtcatc tgcattatga tacgcgatac aaccgtcaaa tacatttcct
    34681 tctcctattg tcagtttggc cgcaaatcca tcggcatctt caaatcctgg atcggcattt
    34741 tcaaaagatg tacagttctt gactaagtta tgcgcgggcc atgcttcgaa aggatcattt
    34801 gagctaccgc tgatttggat tcctgtattt ccattccgat aagcatgaat ctcttcgatc
    34861 aaattatgat ttccagaaat ctgtaatcct ttttgcatag ctgcactatt tgtaatgtca
    34921 aaccctcgta aaccccagta gtgactgaac agtgtcatcc catttccagt tcctttaaaa
    34981 tcaagtacag gacgcttgtt ttctggattt ttctctgcaa tcaactgaat cgggttttct
    35041 cttgtcccgt taacgccttt tggaatcgtc aatcctcttt cgaaggaata agttccaggc
    35101 gccatgacga ttgtctgccc agcatatgcg tatctcaatg cttgtgtcag agataatgga
    35161 ttttgtctgc ttccgtttcc tgcatctgac ccttctggtg ttacataaac tgtcgaatat
    35221 ttaggtttac ggtagtccac tgtatgaaga atctcttttg tttcataact agtcatctct
    35281 acgtattctt ctggcggata gtttttatct ggcgtaaaac tgaaagtaaa ttcgttcgtt
    35341 ccttccatca gttttgtctg ataatcaaat tcccgattcg cagtgatagc caagtttttt
    35401 gcgttcttaa gcacctctcc tgttgcttta tttttaattg tcaataatcc gtcagcattt
    35461 gctctaaagg acatagtatg atcttggttt cctgaatgat tactagaggt cacagtgtaa
    35521 atcggatcaa tcaagcgagt aggtctttct tgcccaggag gatctgtcgc aggatctgag
    35581 taagcaactt cgattccttc taccttgatc gtcatattcc ttgctgcagc aaatcctgca
    35641 taaataaccg attcatctga agcgtataat ttttcccagt catacatgat gtcagaagcc
    35701 atttcttctc ctgtttcagg atcgtaataa tatgcctcta aaccagtatt cgtttttttc
    35761 atctctacgt ccagtttagt aatcgagttc gtggccggaa acagattccc atgaccgttt
    35821 ccgaatatat tataagaacc tgtttctttt tccaagaacc ctgctgaagt ctctaaaggt
    35881 gttgtttcca ctgagacact cccaggagct ggcgcagctt gcggatcttc agatgatatt
    35941 cctgtgattg cccgtgtccc caatccaagt cgcatcgtat atttgctgcc gctcgtgttg
    36001 accacttctt gtctatccga gtcccagaca tattcgatcc gactgcctag tatggcaaat
    36061 gaatttgaat aaaatgcctg cccaaacaaa tgattttttg gtactgcgtc tcttaccatt
    36121 aaagcaaatc cttcttgggc attcgtatag gtccaagatt ctatttcgat cgtcgcccgc
    36181 atcctaaagt tgatgtcttt ggggattgct tgataataat aggtcattcc atctgatccg
    36241 cttgactgga attttccgcc gttattcgtg gaagagagcc gcacgccgtc ttctaatggt
    36301 tcaatggcat acctactagc acttgtgctt tctccgaaaa caaccccttt ccactcttca
    36361 tttgcttctt cagattgctt ttttgtcttt tcattttctg attctttcat cataggatca
    36421 tttccttctt ccgtatccat atcttcacta gttcttggta tttcctccat tgttgacatc
    36481 ccttcaatgt tcacgtgtgc attttcttcg ttcacagact gttgttcgtt agcagcaact
    36541 tcaacgacag aaattaaaac cggggtactg agcataagga gattcatcat tagcgcactg
    36601 tattttatac gtttttttaa caagtgtatt cctcccgtaa caaattagaa agcgtttact
    36661 taaagtatta cctaatttat ttttactgtc aataaaaaac aaaattataa taagtgtatt
    36721 ttataaaaag aaaaaatata acttaagttg ttgataataa agagatttat taacggaaaa
    36781 agaataagtt tttttaaaat aacgtataaa aaagagcata aaacaaagtc gtttgtttta
    36841 tgctcttctt ttattatttt gtgcttttta ttttatcaaa tcactttgtt tttttataaa
    36901 atttctgcgc ctagtgtgga ctcaaaatgt ctcaatgccc attcatgtcc aacgggatcg
    36961 aaacttgcca cggaatcttt gaaaatatgg agtttgtatc ctagattata cgcatcgact
    37021 gctgtatgca acacacagat atctgtacat acgccggtca aataaatatc tgtgatctgg
    37081 cgttctcgca aacggatgtc tagatctgtt ccactaaaag cggaataatg ccgtttgtct
    37141 atccaataaa cattttcctc ttgctcatag tcttggtata aactttttaa agagccgtac
    37201 aattcccgtc cacccgttcc tatcacatta tgcggaggaa ataagcggtt ctccggatga
    37261 aaggaatctt ttggatcatg cgcatcaatg gcaaaaacga caaaatcctt ttgatcaata
    37321 aacttctttg tatgagcagt cagctttttt tcaattactt gtcctgctgt cccagtagtc
    37381 agctttccat cctctgcaac aaaatcatat gtgtagtcaa tcgagatcaa tgcgttcatc
    37441 ttagtcctcc ttatacgaga tgtttgacaa attcgatcac catttctgct gatcgtttgc
    37501 ctgcgtcttc gataaattcg tcaaagcttt gtgtcgccga gtgatctgct gtgtcgctca
    37561 ttgcacgtac gatcaaaaat gggatattga actgacgagc tgtttgtcca acagcggctc
    37621 cttccatttc gcaagctaat gcttcaggga aattcgttaa gatttctttg attttatctg
    37681 gcgagtcgac aaatgtatca cctgtaacga tcaatccttc ttttgcgttc aaattcgttg
    37741 cttttgctgc tttaaccatt tcactgcgca aatatgtgct tgcttcataa tatagaggca
    37801 ttcctggtaa ttgtcctggg ttgtagccaa atcctgttgc atctgcatca aaataagcta
    37861 ctttgtctga tataacgatg tcacctactt gtaagccttc accgattccg ccagctgaac
    37921 ctgtattgat caccatgttc acgccgtatt gttggatcaa cagacttgtg gtaatagatg
    37981 ctaagacttt tcctataccg gaacgtacga cgatcacttc gtggttacct aatgaacctg
    38041 aaataaataa agcgcctgcg cgttcccatg ataatggttc gcttaggttc tctctcaaaa
    38101 tttttacttc ttcttccatt gcgccgataa taccgatttt catcttttta attccttctt
    38161 tctatataaa tgcgattgct aaaaaaacca cacataacat taggataaca ataataagag
    38221 atttcatcaa aaaagagttc catttggtcg tcttttcccg ttcgctgatc cttgttttcg
    38281 tgattggttt attctttttg ctttctttac gataaaagct ggcaatcttt ttttcttctt
    38341 gctgataagc tgtttctgct tttcgctgtt tttttaatga ctcgcttgct tgtgcttgtt
    38401 gtcgcttgcg aagttcgctt cgcgtaataa gcggtccttt ggccataagc cgatccctct
    38461 actttccttt ggtcaatagt tcccagtatt gaatcgcata aatcgtcttt gcatcacaaa
    38521 tcaactgatc tttcatcgct tgttgcgcct cttcaagagt tagatgatac aactccagta
    38581 cttcgtcttc atcttgtgct aaaggattct cgaccttctc taccccttgt gcatgataaa
    38641 tatgaaggac ttcatttgca aaacctggtg aaagatacat cgaagtaaga tggctcagcg
    38701 acttcgcacg atagccagtt tcttcttcta attctctagc tgctgtcatt tcgggatttt
    38761 gtccttctcc tggatcgatt tttccagctg gaatctccaa aatcactttt tctaaaggtt
    38821 ttcgaaattg tttgacaaga agcagacgat cttgttcgtc aaaagcaatg atcccgacac
    38881 cgccaggatg aaataccagt tctctcttcg aagttcctcc atctggcaaa cgaacttcgt
    38941 ctacagcaac atcaataatt tttccgctat agatttcttt gcgactgatt gttttttctt
    39001 caaattgctc attgttcagc atatcttctc ctgctttctt tgtaatcata actgcttttt
    39061 tttcattttt caatttttct caaaaactcc ttattagttt accatagata aaccttagat
    39121 tttctctaaa tatagaaaat ccttctttag tccgatactc tttctagtag tccgtatgct
    39181 aaaataagta tgtaatcgaa taacgaaaag aattcaatac tcaacgataa atgacatgga
    39241 gggtttatta tgataaaacg aaattggccg tggcttctag cattggtctt gctatttctc
    39301 tttgtccgaa acaatagttt ttcttttgtt ctaggacttt tattgatagg tgtagtttta
    39361 gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa aacaagaaga aattcctgca
    39421 ctgactgacg aacttgaaac tcactatgaa aaaagcggta tgacagcgaa tgaaatttca
    39481 ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca atcaattgca aatgaatatg
    39541 cagcagacag ccaaacttaa agcaatcgat ctccgccatg atccagtcaa agcagcgaaa
    39601 gctttattca aagaattggt gaaagaacca aaccgactgc atgaagcaag tcagtttctt
    39661 tatacgcatc tgccgaatct agttgatttg acaaataagt ataatgagat caatgaccat
    39721 gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa gcgcgcagat catcgaccag
    39781 ctgtcagctt taatcgcaca agattatcaa aaattcgttg ctgaggatct agatgatatc
    39841 gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg ataataaata tgaaccagaa
    39901 caaaaggatt aactcttttg ttcataaaag aagactcgga acattcagat gatgtctggc
    39961 tgtttcgagt actttttcta aagtagagac attcatgtaa gaatcgagaa cagattcaac
    40021 aaggaggaat tttttatgga tcataaaccg actgaaacaa aagatgttac accagttaac
    40081 gatacacttg aagatttatt gaacaacccg ttttcaactc caacagatac gctgaccact
    40141 tcacaacaag cggagattga tcagctccaa gagcaacaga cagctgctcg tttaattgac
    40201 aaactaccgg cggaacgcca agaacaagct aaacagctag cagcaaaaat cgatgctaat
    40261 gatgcacagt ctgtcatcag ctacggatcg gctgcccaag caaaattaag tgaattctca
    40321 caatcgatgt tgaatcatgt gcaagcacaa gatattggtc ctgtaggaga ttccttaact
    40381 gaactgatgt atcgtctcca agaagccaat cctgacgaac tgcgtgccgg agaagggaac
    40441 atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct atgaagtgac agcaaaatat
    40501 caaaagattg gtgcacaaat tgacaagatt tcagtgaaat tagataaaga aaaagacggt
    40561 ctgctaaaag ataatctgat gttagaacag ctttaccaga aaaacaaaga ctactttgat
    40621 gcactgaata tttatatcgc tgctggagaa ttgaaaatgg aagaacttca aacaacgatc
    40681 attccagaag cgatgaaaag agcagaagaa actggcgatc agatggatgt tcagattgcc
    40741 aatgattaca cgcaattcct agatcgtctg gataaacgta cgcatgatct tcgcttagct
    40801 agacagatca cgatccagca agcaccacaa atccgtttga tccaaaacac aaatcaggcc
    40861 ttggctgaaa agatccaagc atctatcgct acagccattc cgctgtggaa aaaccaagtc
    40921 gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa ctgctcaacg ccaagtctct
    40981 gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc tgaaaatctc agccatcgaa
    41041 acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga cccttcaaac cactcaaaat
    41101 gatttgatcg aaacaatcca agagacattg cgtatccaaa aagaaggaaa agaaaaacgt
    41161 cgtcatgcag aagtagagct tggacacatg gaagaagatc tgaaacaaaa actgttggat
    41221 ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa cgcccaacaa cagtttatta
    41281 caactgttga tgggcgtttt tcttatcttt cttctaactt ctccattgga gtctatttct
    41341 cattcatctt gctgtgtcca tagccatagc caaaataaat gatcagtcca ataaccatta
    41401 cgataccgaa cacgatccat gtcacagttt gcagacgaag catcaataca atacaagtca
    41461 agatggagac gattggcaat agcggaacga atggcacttt gaattctcca gcttttggtt
    41521 tgccaaacat ttttctcaac ttcagtaagc ctagactaac aagcatcaaa taggcgagcg
    41581 ccatgatatt cgttagttcc gctaatacat tcaatgggaa aaatccggca aagaatacag
    41641 ctgccacacc agcggcataa gtcgcttttt tcggtgtacg atgcttttga tcgatttctg
    41701 tcataaactt aggaagtaag ccatccttgc tgacagcaaa cagtaaacga gcaagtgaat
    41761 acatcatcga gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg atcaccgcgc
    41821 ccactgttcc atgtcctaca tacctcattg caaaagccac tgggtcattg actcctagtt
    41881 cagtatacgg aacgattcct gtcaagatca gcgtcacgat gatgtaaagg acagtagcaa
    41941 tcagtatcga accaatgatt cctcttggca catctttttg cgggtttttg acttcctcgg
    42001 ctgccattgc aacggcatca aatcccaaga aagcaaaaaa cacaagagct gcaccatcta
    42061 gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta acgaaaaata
    42121 ttcctacaat gataaacaac gcgatgatac caaatttgac atataccatg gcattattca
    42181 aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt gacgaatatc actacgagca
    42241 ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta cccgccagtt aatgcttgtg
    42301 gaagttcggc ccctaaactt ttcaaaaaac cttgcatata gccagaccat ccggaagcaa
    42361 cagaggaaac cgctagtaaa aattccccga tcaacaacca ccctgtcagc caggcagcaa
    42421 attcaccaaa tacaacatac agataggcat atggcccacc aatcactggc actcgcgaag
    42481 caaattctgc gaaacttagt ccagacaaaa tgacaacgat tgcagctaag acaaatgaaa
    42541 gtgacagtgc tggaccagca ttttgattgg ccgcaacccc tgtcaccaca aatattcccg
    42601 taccgatgat cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt tcttttttca
    42661 tctcactcgt atgattgaga ttgatctctt tcttacgaaa caaatccatt gaagttcctc
    42721 cccattttag gttggaaaac agcgattaaa ctgttttcat ataccctaaa aggataccac
    42781 ctaaaatgac taaaaggcaa ccaacaataa cataaatgag ctcttttttc gtttttcttt
    42841 cgccaaggat gaaaatccca cctaaagtag agataatgat ccccatttgt gaaagtgaga
    42901 agccaacagc taaaccaagt gatttgactg ttagaagcat gcagatgttt cctaagcccc
    42961 ataataatcc acagatcatg tttttccaca caaactgatc gactttaact ttacggaaag
    43021 caaagaagct tgcgcctaag atcattccga tactttgagg taagataatt gacatcgcat
    43081 ccaaattcgc ccagttgacg atgatggtat atacaccata accaatcgta gagaagatca
    43141 atgctctaaa acccttacca aaatcaagca ttttattatc tgtatctatt tttccttcgt
    43201 catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca ataataccgt
    43261 atacataatc tttcgtttgt gtccattcat ggaagaaaac ggctcccgca atcgtattca
    43321 aaattaattg aaaaccagta gacaatggta gccctacaga aacacccatg tacttcattc
    43381 catggaactg tccattttgt ccaacactcc aagctaagcc ggataaaaat ccgatcaaga
    43441 aaatccaagg ggtgattaca ggactaacaa cgaaaaagac aactaaagaa aacagaaatg
    43501 caccgatcgt cattcctagt gtttgttgat tggcatcgcc accaatttta ccacttacta
    43561 aaccaatact accccatgcc aacataggga ctaatgctat taaaatttcc ataaccaatc
    43621 tccttttatt tcacatgata agaaacttat cagcgaaaac gttaacaaca caagcacttt
    43681 ttttcgtttc taacgatttt ctaacgtttt tcattataaa aatttaagat tgggaaaaat
    43741 aaagtcattg ttgtcccttt tccttcagtt gattcagccg tcaaatggat acctaatttt
    43801 tcggccagat tttttgctaa ataaagtcct aatcctgttg aatgtgtttt gctaagtcga
    43861 ccatttctcc cagtaaatcc cttatcaaag atccgtttca agtcttccgc tgggataccg
    43921 atccctgaat ctttaactga aaggctgact ccttgcgctt ccttttctat gctcacaatg
    43981 atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt gaagataaaa
    44041 gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc gtagcgaata
    44101 tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat tccttttagt
    44161 ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata atacaaaact
    44221 tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata cttttgatcg
    44281 tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg cactttgatt
    44341 tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat tgctgattgc
    44401 attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag tttttcttct
    44461 tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat gtcggctaat
    44521 tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat cagaaagacg
    44581 ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc
    44641 cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa tgtccactga
    44701 tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga acgatatatc
    44761 cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt tttcggcgta
    44821 accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca tcccacagtg
    44881 ctcgcagcaa tttttcccga cttaagatct ttccattgtg cttcatcaaa ataaataata
    44941 gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata cttccgttgt
    45001 caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct gtgtagttgt
    45061 aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg aaatgttttg
    45121 tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc gtattcctgc
    45181 tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac caataatagc
    45241 catccaaaac gggaagatta atatctagta atacaagctg tggttcttct ctttcaaagt
    45301 ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat ttctcaagtt
    45361 cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt tttgccataa
    45421 aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg tgaaacaagt
    45481 tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa tatgcgattt
    45541 aatcgcgttt gtctcttcca agcagcttaa atttattatg ttattcttaa taattggtct
    45601 attatctgtt atttttcttt cgttgttttt gtcttttaaa ttttcgataa caccaataac
    45661 cgaaaagcgt tagcactagc aatataccta gaaggagtat aagtaatcga taaagatggt
    45721 atttttttgt ctgttctatc tttgaagaga cttcttctgt aaagggaact cgtcttcctg
    45781 tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt gtgactagat
    45841 cctgcttttc tctaattgct aatgtatttg tttttgttgg aaggacaaca attttctcaa
    45901 cgacttcata agcgagcatc cgaccataca cttcaagata gaatcggtca tcttgctcca
    45961 tttcttcaag atccgtaaag agttttttct cagcaatacc gctatgggcg gtaatcacgg
    46021 aatgtgtact ctctcctcca gttggaaaag aggtgccttg caggactgtc gctcctctat
    46081 ctaacaaaag atcattcgtt tcatcaaata aaggcaagct aacattgatt ttcggaatat
    46141 agatggctcc gatcatatgt tgttcgtaat attcttttcc tggattttgg acatttctaa
    46201 ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg gattactgat gtatgagcaa
    46261 gcgtctgatt tttcttttca tgcactgcta attgttgctc atgtttttct tctttatcgc
    46321 gttcaagctg cttttgataa ttctcgatcc ttctttgatc caaataattg ctgagagcat
    46381 ctgctaaaaa tggatatgta aaaactgcag ctccggcaag aaacatcact gccataaata
    46441 attgtaataa aagactgatt tgggtttgag gtctattacc tattttcttt ttcattttct
    46501 tcaccttctt atttaaaaaa actgcagcaa cctttataaa accgattgct gcagcttatt
    46561 tttgttgctt atacttcaga cttcatttta gatttacggt accagctgta cgctccaatc
    46621 atcaaactta ttccgattag taagaatgcc aagagcccgt tcccaccagt agcaggaagt
    46681 gacccgcgtt tcatattcgg tactctttct ggccctggat tttctactcc tacgatctga
    46741 atttcttcat tatagcttcc ataagagatg ataaattcag taaaggctgc ctcacctgtt
    46801 ggaagtacat aaccttctgg cgccatggtc tctttcattt gataggttcc ataatctaac
    46861 ccttgaacct caaagtatcc atttccatct gcgacaaatt ctgttgcttg tccgcttgtt
    46921 gcccactcaa ttctatctgc tcctgattca taaacagcta aaggcgactc ccctttataa
    46981 aatattgcgt attcagaacg gtcttcattc cacaattcga atcttgcatc tttaagcaat
    47041 tcactgcttg atgcatcata tttttcaaac tttcttccac ctgttgtgac tttctctggc
    47101 ggctctactc cttcttcata atcaaattca tcatttcgtc cgatttcaat aaccgctgtg
    47161 ttgttatgga aatcatccgc ttctagttct gtggaaacct tcaaattata cgtgatcgtt
    47221 aaatctcctc cacgtgcatc tgctaatcta cctaaagtat ctactttggc tactccattt
    47281 tcttctgtca aaatcgtcac gacaaacccg ttgcttacta cttctactat ataatcttgt
    47341 ccttttgtta aaatcgtctc gccggctcgc acttctattt cctgatcata ataagttaga
    47401 ccaactgtag gcgtatctgt gattttgtaa tttttgataa cagcgcttcc gttttcaagg
    47461 acagaatcga tgaaataagg aataggtgct gtgatcgtgt aagaagccgt ttttcctctt
    47521 tctagatcaa tataagaaat cgtttcttgt tctccagctt cattaataat cgttacttgt
    47581 ctaaggtcgc tttcctctac attcatctgt ttatctgctt ctctgatttc gtttttcgga
    47641 tacaaatgca catctgtatt gtatgtgtct ttccatgtag aaccatccca cacgccatcc
    47701 accatatcag gcatcatcac tggcatcata acaaccattg gagacgcgat tctggaaata
    47761 tgagctggcg aataagtttc taagaaaaga tagactttat ctctagaaga aggtgcttct
    47821 tccgtcactt ggacggtaga gaataccgct tctccagctt cattcgttgt ttgtttgtca
    47881 atacttgtca gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg aatccattca
    47941 atcgcttcgg cagaattaag accgtcttct cgttccggtg ttaaaggatt atctcgaatc
    48001 aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat cgactcctgg aatattttct
    48061 tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt cttccggaac agatgagaaa
    48121 ccttttttgt gtaatgtaat cgtcacttca cttggttttt gtgcgacttc ttcggctttg
    48181 actagttttc cgttcccgaa aatccctatt cccgcagtaa cgagtaacag tacactcatt
    48241 cccgtgacaa tcattttttt attcattttt ttctccccgt tcttttttta ttaataaaac
    48301 agcacctaac aataaaagaa ttcctagtaa agaaatcaac gttcttgctt cattcgtttg
    48361 cgggaatacg gaccattttc ctgattgacc tggattgctt ggaccactcg gctctttcgg
    48421 tttgtccgtt gtttctttta cggtctcgtt ataaacacgt ggtgttgcgg attcataggc
    48481 agagactgga actttcccgg attctaattc agccatctcc tgtccgttaa ctactacata
    48541 ttgtggttgg ccttttgcat ccacccaaaa ttcagggatg atgacctcga ttctcttact
    48601 tgcttcatcc atctcgtaac cttccacacc ttttaattct tcaaaataat aggtgccaga
    48661 aggcaagaaa cgttgtcctg tcgtaacaag gccctcttta tccgaaatga atttgcttac
    48721 atttttgtca ttcaaaggat cattgtctgc tggtttgatc cattgatttt tcaagtcatt
    48781 agcaggagcc atatctaaat aatatttccc cccttctatc atctgatata gagcaaaaac
    48841 agcaccttct aatggttttc ctttttcagt tgttcctctt tgacgtccat acttgaagaa
    48901 gtatggatct cttagataac cgacattttt aggatagata tgaatctctt gcagttcctc
    48961 ggttgattct gtcgggtgat aaattggcaa aatagcagca attgggatag ctgtctgatc
    49021 gatatcaaca tccaatccaa cttctggatc aatcgattct tcgaaaatca aataaactgc
    49081 atcccgatct tggtttttct taggtgcttt gatacgtgca atcccgtctc ctaatccgcc
    49141 aaccgtatct ttcccagttg ttaccgaagt ccctatttgg acaagccttt cttctgtgat
    49201 tagctgacgg atattgtttc tgttcgtttc agcgatttgc tggacgaatt ctttaggagt
    49261 catgttttca gcagcttttt tttcatgata ataatcggtc atgtcgtaaa tggcaaatgt
    49321 cgctccattt agtggcaccg tctgtgaaat gatatctgct gtttcccctg cttcctcaga
    49381 gatagccaag ccatcatttt catggtattc aaattgatcg ttataatcga tatctcgaat
    49441 catccgttta tgcaacacga attccacgac atcttcccct gattcttctg ccgacatcgc
    49501 atgtggaaga aggactgctg caagcggtag taaaacaagt aattgaagca tcaaacacaa
    49561 cagctttttc attaccttgc tccctccttt ttagattgtc tgacaaacaa accataggct
    49621 gctcctgcaa gcgccatgaa gaatgcagca agcaggatat agaataatcg tccggatcct
    49681 cctgtagcag gcaacggatt ttcaggatcg ttcccgacac taagctgtaa tctgattcga
    49741 tttgtctcca aaagttcata ttctaactct tgctgtagat caggcgtctc tgcttcttcg
    49801 tttggataat acgaaatctc aacttttagt gtacccgctt cttttcctgg aactacccga
    49861 tcattttcta tgtcaggttc ttctcgataa ctaatttcta aaataaagta acctggaagt
    49921 tttctatagc tgtcaggtga cttcacttcc ttgatccgat agaccccttc caacaagtct
    49981 gaagcattga atagggaaga atcgtttgga tctggtgcca agcctccctc gatgatttca
    50041 tactctcctg cactattttg tttctcaatt gaaaattctg cgccttcaag acgtcggcta
    50101 ttcatactgt caattttcaa aagttccaaa tcgattggcg ctagttcatc ttctaaggtt
    50161 acttgtagta caccatcgat gatttcatag ccttccggca agggagcagt ttcgtcttcg
    50221 atgaccgtat cttcaatgat aaacttacct tcttcattca gtgcaaactg gaacacttga
    50281 tcagatagcc gatacccatt tggtgcgaca gattccgaga cctcatattc accaatagac
    50341 agatgcagag gttggtcgta atttaaatcg acatactttt ctactcctac ggcacgagaa
    50401 tagaagtatc cgatcccagg caaacgtttc gattcttctg gttctaacgg attaccagtg
    50461 tatgctcgat acagcgggaa ggaagaatcc cccactttcc tgatatcgaa acctgcacca
    50521 tctaatgtat ggccttcatg gtctaatttt ctaactcgga tctcccgata atcaaacgta
    50581 ttgtaagcag tgaatgctac ccataaacga tcctcttcat ctgttgtata cgtgagatat
    50641 tgccaatttt cttcacctgt ttgcttgagt tggatagtcc ctatagcatt atcgtaaagt
    50701 cgcaagatcc atgtgtcatt ttcatcaggt tctttgaaac cttctgggtt ctggctctca
    50761 atgattttgt attctgcata gttggtagtt tcttcactat ccttataaac aaatgaatct
    50821 ggctctccga ttggatgaat aactttctca ccatccacga ttacatattt cccgtcttca
    50881 tctactgaat agaacgtgag tctccctttc tcttcctcta cttcgttcac accagaaagc
    50941 cctttcgcta ctggatatcc gatttccagt ccgttaccat ctgtttcata aagttcaaac
    51001 tctgcgccat tgattggcat attactgcct tcaattagtt tattgatatt catgtggatt
    51061 ggcttgaatt catttcgcat ttccagtttg acatacaact catcatactc tgtgccttct
    51121 atgttaggct cttgtacaac agaaagcttg tgatattctc cttcataata ggaattcgga
    51181 tctttacctt ccggtccgcc gactctcgaa ccagatgtat attcatctac aagctcgtag
    51241 tgacctaaca tctcatcttc gttggctgca tattttttga tttggaattg atacgtctca
    51301 tctactaact gataacgtgg tggtccagcg atctctctga tccaaaatat gtcgctgact
    51361 gctccagttt catctcctaa aatcggcatc ttcgtttcaa agatcgctat tccgtccgtg
    51421 gtagttttac ttgtttcgat tggttcggct tccccactct ctggattttg gtcatataat
    51481 gcaaattcga ctccagccaa cggctcgcca ttttgaccct tcttgctgac ttggaaatta
    51541 cggcctttat gttcgtaacg aatggaagaa gatgggaaat acatccgtcc agtagtgata
    51601 tcattctcat aatcctccag gttctgaata taggccatat tgttcactgt gcaaaaattt
    51661 ccgtcttgag ctcccgcatc aagttttact tggtacttga tcctgaccat ttccccttca
    51721 cctaaagaaa tcggagaaaa tttataagct tggttttccc gtccgacata cgttgtcgga
    51781 gtcgccacta cttctggtgg ggcttgtcca tgacgttctt catcccatat ttctggctct
    51841 tggtacttca tgccgttttc tcctggatct agatggtaaa actgaatatc tgccgatctg
    51901 ccagctccaa atggtgagac attaaaacca gtttcatcat aaagtgtggc ttgctggatc
    51961 gttctcttga aggtagaaga gatatcctcc aaggctgtaa caatgtcatt cttgttgttc
    52021 gcattgtaat atcgcggtgt tacgccatct tcttctaaat cagcaatatt tttcaataca
    52081 tttcgtccaa tagcatctag tcgttgtctc ccgctaacgt ttccatcgat tcctaaacca
    52141 atggagaaga tacttggcgg gttctcttga tacttgtttt tcagccaatg atgataagca
    52201 actgtgttga tcgcagatga cttttcttgc gaagtaggac tgccattcgt attgttatta
    52261 gaagaaccat caccaaccca gtgagtcgct ttattatctc cttcttttac ctctccataa
    52321 cgatagtgta catttacttc atcttctgga cggttaaagt cagatgcata ggtaactgga
    52381 tgagtaaacc ctgcattaga agtattccca gaaaactcct caaaatttct gaacaaaacg
    52441 tcattatctt cagcaatctt atcttccatc acagtccagt tgctccaagc tccattagtg
    52501 ctcgtacgat aacttgactg aatcggtgca taactgaagg tcggtgtccc atctcccaca
    52561 acgattagga ttttttctgg atttctattt tctccgccat tgtctttata caatgtttca
    52621 tagccatttt taagccctag tgtaagcgga gttcctccga ttggtgtacg atttaatgct
    52681 gtattctcac ttcccgtcaa acgattgata tcatttgtca atggaaagat gtccgaactg
    52741 atttgctcat ggttattgat agctgtacta ttatggtaaa aattcaccat accgattcga
    52801 atatccagac tcgggtcaga gagcagatct ttactcattt cgtttactgc ctcaatcaac
    52861 gcagcatttt tagtttgact attggtccca gcggttaatt cactcatact cgctgactta
    52921 tctagaacaa aaacgatatc gattggactg atttcctttt tctcgccccc gatgacatct
    52981 agatagacat tgtaaagggt tgttgcatct tcaggatgta gatcagatgc tgtgctgctt
    53041 ggcagaacag gttctaccca ttttttgatg tacgccccgt tataatccaa gtaactgttt
    53101 tcaaaattcc gttcatttcc ccaaagctga acggtttgag ctggaacgtt tctatcttct
    53161 tcgcctgcag ttgatccatt attagtagca tagtcgtaat ttcgccagtt cgctcccccg
    53221 tttgtataat cactatcatc gatcaagttc tctccgtcat atggcgagcc tttgtctcct
    53281 tgatagcgtt cattgctttg tgtccaattt cctaacactt ttgtatagtc attcgttgta
    53341 aacttcgggt agcgatgctg ttcgttttca acttctccgc taggatagat atattcatag
    53401 gggtcttgcg tagctgttgg cccaagacgc tccaaaatac cgttgaataa ctggcgttga
    53461 atgcgctgga gtgcttgacc gctgggtacg attggttcgt ccagcttttc catctcattt
    53521 tcttcgctac gttgaatttc agcggaatct tctgtatcag tttcattttc ttgtaattct
    53581 tggttatcta ttctttcatc aaccaccact tgaagaggta gttacccttc gttcacatcg
    53641 gggatctttt ctgtttcaaa aatcaatgtg ccggtttctg cttcttttga ataaactttt
    53701 ccgacatacc attcttgacc atcgatcgtc tcgactttta ggtcttctcc atcttcatca
    53761 taggaggata gacctgttcc acgaacgttt tttacggtac caatcccggc ggctgccgca
    53821 tcgattgcta aacgtattct accttcattg gcactttcct ttttttcaaa agccagatgc
    53881 cattcaaggg tttcttcttt ttcaatatac ttcccttcaa tttttgcata gtcctcatct
    53941 aagaatacct catcctcctt tgtttctgtt gactcagcag ctacatccag ggtcgtcata
    54001 tgatagactg actggaaata tggtccaaat agcaaactga cagttactaa ctgcaaaagc
    54061 ttcaatttta accctttcac atccttatac tcctttcaaa aatatttgtc ttctttcaca
    54121 acaatcaaca aaacaatgcc catctaatta taatttaatt taattttaat tttaaatcaa
    54181 atgatatcct ttttagttaa aaaacaccaa ttatacaaca tttattatta aaattaaatt
    54241 ataaaatttt ataacgttct catttaacaa aacttaagtt attaagacaa atgaataaac
    54301 aactacgctt tcctcgaaac aaaaaaataa agcacaaatg cgcatttacg cactcatgct
    54361 ttattcgtac aattaattca gataacggca gcgcatagga tgccaataac acttcctaaa
    54421 atagctcctc ctaaaatgtc agtaggataa tgtaaaagta aaaaaatcct cgaataagcg
    54481 atcaaacaag caaataaaaa ggctaatatt cctgcaactg gacttaattc gaacaacgca
    54541 gctactgata aaaaggctag cgctgtatgt cctgaaggaa aagaagaagt cttcggttta
    54601 cttgtgtaaa ggacaagccg acgatttttt tcataaggtc ttttccgtct tgtcgttttt
    54661 ttgataaaaa gcaaaccaac aaaaaaatag atcaaactgc cgataagaat ggctagacca
    54721 tattctctag tatcaggtat caagactaaa acagcagcaa ggagcaacca aataaaacca
    54781 ccattagaga tattactgat aaatttcaaa aaattgattt ttcgactatc cgttttccgt
    54841 tgcttggcga acttgttgat aatactatca tcgattttag caatcaaacg caacatgtta
    54901 ttcctccttt ttaagaacct tttcttgcgt acgatggata aaaagcagga cttgaaaata
    54961 aaacaagaaa ctgaaaccta ataaagcaaa atagattggt cctgcaaacc agaatgcgac
    55021 aagaaggatc aacgtgcctg ccaaatagaa aagcgcgctt ttgaaatcat taaaaaacaa
    55081 gaaaaatgca gtaatccaag aagcttcttt ttgttccagt tgaagcaaga ttttgtaact
    55141 aaagagaagc gcgcttaata gaagcaaaaa caacgtagta tacatcaatg tatagccaat
    55201 tcccccaccc caatagcgga aaatctgata atcgatcaat agaagaagaa aataaacact
    55261 tgctaaacta tcttgctttc cataaagaga gaagttctca cgaaactttt tccagaatag
    55321 tgaaccgagc tttttctctt catttttttg taatgataaa tcatatacag aactgttcga
    55381 cttgcagttc ctaatgtgaa aacaggcaag acgcacagga accatagtag ctgtaaatgg
    55441 ataagacgtg gaatccattc aatgatttct aagaaataat cattggcttt tttaccaatc
    55501 gatcgattca tcatcctttt gaacctcctg tcaaatccat cccttcaatg aaataacgtt
    55561 gtgcaaaaaa gaacagcaaa atcgtcggga tcaagacaag tgttgctcct gccatcagat
    55621 aattccattg tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt aacgtgtatt
    55681 tttcttgatc ttggatatat aacagaggac ctaagaaatc attccaagct gcgttgaagg
    55741 aattgatcgc aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa tgactccaaa
    55801 tatacaaatg attcgctccg tcgatttcag cagcttcgat caattcatta ttgatcgata
    55861 agtaaaattg acgcatcaga aatatattga acgcattgcc gaagaacgat ggaacgatca
    55921 ggggcaaata agtacctacc cagccgatct tggagaataa tacatattga gggatcatcg
    55981 ttacaaaccc cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc tttttcccag
    56041 gaaaatcaag cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca ttaccgatca
    56101 ctactagcac agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga aaggcggcaa
    56161 tcgtatccag atagttttcc catacgattt tttctgggaa aaaggtcgga ggataacgaa
    56221 cgatttcttc cattggtttc aatgaagtaa acaccatcca aagcaatgga atgatcaaaa
    56281 tgattcctat tactgtcatg aggacataac ttagtatttt tttgtttgcc gattcacact
    56341 ggtctttttg aaataatcca tcatttgccg tcacctccag aataatagac ccacttaggt
    56401 gcaagcttca aattgacaaa cgtcagaacc aacacgatca gaaacaggat cattgacata
    56461 gccatcgcat aacccatatc aaaagctacg aacgccttgt tccacatatg cagattataa
    56521 aaaagcaaag aatttgcagg tccgcctgtt ccatttttcg tcatgacata agcttcttga
    56581 aaaatttgga aagaaccgat cgtagaggtg atcacatcgt aaaaaataat aggagtgatt
    56641 aatggcaaag taatactttt gaactttccc caaacgcctg ctccatcgat ttctgctgat
    56701 tcatataaag atctaggaac attttgtaat gtagctaagt aaagtagcat tgctcctcca
    56761 gaactccata ttttcatcaa aatcaacgca ggtttcgtcc attgtggatc aaacagccaa
    56821 gctggtccag ggatatggaa ccagcccaga aatgtgttga tcaatcctgc agatggagaa
    56881 agcagctgca tccataagat gtagactgct actcctgata agactgccgg taaatagaaa
    56941 gctgtacgga atacactgat tcctttgatt ttttgattga gtactgtggc taaaaacaca
    57001 ctgaaaatcg tcgtcagcgg tacattgaag atgacgaaat aaacggtgtt gtataacgat
    57061 ttccaaaata aatcatcgtg aacgaacatt cttttgaaat ttgctagacc aatgaaattc
    57121 atcttggatg tcaaattata atcggtgaaa ctaccaataa ctgaaaaaag cataggccct
    57181 agcataaata ccaagaaccc gataataaaa ggcgagataa ataaataggc ccatcttgct
    57241 tcttttttct ttcggataga taaacgcttc aaattttctt gcctcctctg tacctagaca
    57301 gtcaaaaatg gattattgat tttgcttgac taagttttct actgcttttt gtgcatctgc
    57361 caatccttct tttggtgatt ttgttcctaa aagaatctgg tcaatctgtt cattcactaa
    57421 tgtcgtgtaa tccggagcat ctaaaggaac tggtgtgatc accgtatttt taaagttttc
    57481 gtccgccatt tgatagatca tttttccttt atccgttagg ttgtcattat tcactaaatt
    57541 ctcattggct gtcttgctcg ccatgatatc aaaacttttt tcgccgaatt tttcttgtac
    57601 ttctggagaa gaaagatatt tgatgaattc ataagaggcc tcaggatctt ttgctccgta
    57661 tggtacttcc aagacgaatc cgccacccca cgaccaatgg cctgaacctt cttctttttc
    57721 tgggagctgc atcacgccaa aatcaaaatc atccggtgca ttttccatca aactagagta
    57781 ataattgata ttttgggcac gcatggctac taatccggaa ataaatggat cagatacacc
    57841 agacccaaat tcggcttcta atttattgat cgtatttttc ccataataat cttgccagtc
    57901 ttggatccac tccaaggctt ccactttatt tttagtatca atcttgactt tttcatcttt
    57961 gtcaaaccaa ctagtcccgt tatctgcgtt tagcgcccat acatcagccc ctaaattcca
    58021 taatggataa aaaccgatgc gtgtaaattc ttttccttct tttttatcta gtttacgtgc
    58081 tgtttcttct aattcatccc aagttgtcgg tagatcttgt tcactgattc cagcttcttt
    58141 aaatatcttt ttattataaa aaatgacttg tgtatctgta ttgaatggga tcgcataagg
    58201 atcaccttca tattctactg tatcccaaag ctgcggataa aaatccttag aagtgtcttt
    58261 ttctacatat ttggagagat tagttgcttg ctgtgcttct gctctttggg caacggaatt
    58321 tatgtcctgt acaataacat cgggtgcatt tccagctgtt attgcagata aagatttagt
    58381 ccagatgtca ccccatggct gatacttata atttacatgg atctcgtttt gtgattgatt
    58441 gaaatcctca atgatttctt cgatagcttc ttttcgtgca ccggatcccc aaaaggacca
    58501 gaaatcaata ttttttcttc catcacttgt tttttcagaa gaatttgctg cacttgaact
    58561 ggcatcgctt gcattacttg atccgcatcc tcctaataaa aacaatgctg aacttgccag
    58621 caaccctgta aacaataatt ttttcatatt ttacctccaa attttaatgt aaccgatgcc
    58681 ggttttaata taacgatatc attttgttct gaaaataaca atttataagg tatgattcta
    58741 aaaaaattat taatgatacc aatactttat ataattcgcg agcgctttct ttgaattttt
    58801 taatctttcg tagcagaatg agaatgtatt aaaaatagat taggaggaat tttgatgttt
    58861 agacaccaga aagaattgca gtttgaagca aaaccagatc gtccgaatcc attaatcgcg
    58921 aaatatctgc aggagctgat cggcggccaa tatggcgaga tgagtgtagc tatgcagtac
    58981 ctgttccaag gctggagctg ccgaggagaa gaaaaatata aagatatgct gatggacatt
    59041 gcaacagaag aaatcggcca cgtggaaatg ttggcgacaa tggttgctcg tttattagag
    59101 ggtgcgcctg ttgaagatca agaagcagcg atgcaaaaag atccaacaat cggtgccatt
    59161 atctctggaa tgaatccaca gcacgcgatc gtagctggct taggacctcg tcctgctgac
    59221 agcgtgggca atccatggag cggtgcctat atcattgcga gtggcaactt gttagcagac
    59281 tttagagcta atttaaatgc cgaatcacaa ggaagacttc aagtagcacg tatttacgaa
    59341 atgatcgatg atcgtggcgt aaaagatatg ctgtctgttc tccttgcaag agatagctat
    59401 catcaaaatc tatgggaatg tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta
    59461 gtcccaagta ctttcccgcg tgacaaagaa cgaaaagaca ttgcttatga cttctataac
    59521 ttctcagagg gagaccaaag tagtgaaggt tcttgggcta gtggcaaagc actggatggc
    59581 gaaggcgaat atcaatggtt gaaagaacct aagatcgaag gcgctgcacc aaaattagaa
    59641 ccagtaacac caaaaatgta tgggacacct ccattcaaga aataaatatc tacccgtaga
    59701 aacacccgtt ttttgttcca cacgctttgt gagcaaaact gactaaagtc attaatcata
    59761 accacccttc aaaagggtgg ttttctctag agctataagc tcaaatcacc ggccagcgcc
    59821 taaagacgct ggctttcata ttttatgttc aagccatatt gcctttgcca gttttgccca
    59881 cctcttaaaa ggtttatgta ctactcctta gcccttaaaa ggatcgtctt actctcgtac
    59941 actcagctta tctaacgcac tatcatgttt ttcttgttct tgaatgtact ttttgatggt
    60001 cgcttcattt tgtcctactt ttgtcacata gtatccttcc acccaaaagt gtcgataccc
    60061 aaatttatat tttagatttg cacaatctct tgatgatctc ctgaatattg gctcgatatt
    60121 gattataaat tatttttctt ctatactttg gtgtgaatac aatgtggtat ttacacatct
    60181 actttgtacg ggctaaacta tttcccttta ttgctccacc cgcatagcaa gtggttttgt
    60241 atttcaatcg ctcggcgcgc tcaattaggt ctaaagacaa taaatctaaa aacccgctgc
    60301 ttcactgaca gaaacaacgg gttttcagaa aaaaatgaaa tgaaaaagat ctcttacggc
    60361 aatctatttt atttttttga ttttttataa atggaaaatg cttcaagaag tccaagaaat
    60421 aaagccgtat aaaaacaaga acctatgaaa tctttattta ttagatattc aacagaaatc
    60481 ccgataacac tagccaacaa tatatttatc agaagtgtaa aataaggatg ctttttatag
    60541 aatagaacta gtgttgtttt catagactct cctttcttca tatttccaat taatcacaaa
    60601 ttcacggcat tactagaaaa attaaagggg cgcagaagtg gaaaaatact cttcgacttt
    60661 ccctacaaac caggccatct tgtgtttaat aactatacca aatctaaggt acctctaaat
    60721 ctaaattcca tttgtggcgg tgacctccaa tttttaactc ttctatctcc catccagtat
    60781 acataacctt gatacttcga ccacttatat atcgaacaca caaataccta tccattgcag
    60841 gagtactacc taacttagtt gctacttgca tctaaataac agctgaaaag ataggactta
    60901 ctcttagtaa agtatctgaa ctattaattt ttaaactatc atttttatcc tcaactacat
    60961 gcacatctgt tgcaacttca ctttctgtct catccagata aatgattaag gaatcgggaa
    61021 ttgcaatatc ttcagctgac gctggaatag ttgagctaaa ttagtcatca tatctttatt
    61081 ataaaataac
//
LOCUS       DAZKOB010000007        50901 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000007 DAZKOB010000000
VERSION     DAZKOB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.007"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..910
                     /locus_tag="ACG8M6_000440"
     CDS             65..910
                     /locus_tag="ACG8M6_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0456137.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1191..2411
                     /locus_tag="ACG8M6_000441"
     CDS             1191..2411
                     /locus_tag="ACG8M6_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0456138.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2408..5128
                     /locus_tag="ACG8M6_000442"
     CDS             2408..5128
                     /locus_tag="ACG8M6_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0456139.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5112..6056
                     /locus_tag="ACG8M6_000443"
     CDS             5112..6056
                     /locus_tag="ACG8M6_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0456140.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6125..7135)
                     /locus_tag="ACG8M6_000444"
     CDS             complement(6125..7135)
                     /locus_tag="ACG8M6_000444"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0456141.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7315..7659)
                     /locus_tag="ACG8M6_000445"
     CDS             complement(7315..7659)
                     /locus_tag="ACG8M6_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0456142.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7652..8086)
                     /locus_tag="ACG8M6_000446"
     CDS             complement(7652..8086)
                     /locus_tag="ACG8M6_000446"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0456143.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8224..8961
                     /locus_tag="ACG8M6_000447"
     CDS             8224..8961
                     /locus_tag="ACG8M6_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456144.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8954..10165
                     /locus_tag="ACG8M6_000448"
     CDS             8954..10165
                     /locus_tag="ACG8M6_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456145.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10369..11151
                     /locus_tag="ACG8M6_000449"
     CDS             10369..11151
                     /locus_tag="ACG8M6_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0456146.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11221..11883
                     /gene="trmB"
                     /locus_tag="ACG8M6_000450"
     CDS             11221..11883
                     /gene="trmB"
                     /locus_tag="ACG8M6_000450"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0456147.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11981..13735)
                     /gene="ade"
                     /locus_tag="ACG8M6_000451"
     CDS             complement(11981..13735)
                     /gene="ade"
                     /locus_tag="ACG8M6_000451"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0456148.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13867..14796
                     /gene="comGA"
                     /locus_tag="ACG8M6_000452"
     CDS             13867..14796
                     /gene="comGA"
                     /locus_tag="ACG8M6_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0456149.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14753..15802
                     /gene="comGB"
                     /locus_tag="ACG8M6_000453"
     CDS             14753..15802
                     /gene="comGB"
                     /locus_tag="ACG8M6_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0456150.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15799..16077
                     /gene="comGC"
                     /locus_tag="ACG8M6_000454"
     CDS             15799..16077
                     /gene="comGC"
                     /locus_tag="ACG8M6_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0456151.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16052..16522
                     /gene="comGD"
                     /locus_tag="ACG8M6_000455"
     CDS             16052..16522
                     /gene="comGD"
                     /locus_tag="ACG8M6_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0456152.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16494..16841
                     /locus_tag="ACG8M6_000456"
     CDS             16494..16841
                     /locus_tag="ACG8M6_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456153.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            16804..17229
                     /gene="comGF"
                     /locus_tag="ACG8M6_000457"
     CDS             16804..17229
                     /gene="comGF"
                     /locus_tag="ACG8M6_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0456154.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17201..17560
                     /gene="comGG"
                     /locus_tag="ACG8M6_000458"
     CDS             17201..17560
                     /gene="comGG"
                     /locus_tag="ACG8M6_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0456155.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17732..18739
                     /locus_tag="ACG8M6_000459"
     CDS             17732..18739
                     /locus_tag="ACG8M6_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0456156.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18764..19948
                     /locus_tag="ACG8M6_000460"
     CDS             18764..19948
                     /locus_tag="ACG8M6_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0456157.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20385..21968)
                     /gene="abc-f"
                     /locus_tag="ACG8M6_000461"
     CDS             complement(20385..21968)
                     /gene="abc-f"
                     /locus_tag="ACG8M6_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0456158.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22073..22201)
                     /locus_tag="ACG8M6_000462"
     CDS             complement(22073..22201)
                     /locus_tag="ACG8M6_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456159.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22546..22689)
                     /locus_tag="ACG8M6_000463"
     CDS             complement(22546..22689)
                     /locus_tag="ACG8M6_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0456160.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22912..23391)
                     /locus_tag="ACG8M6_000464"
     CDS             complement(22912..23391)
                     /locus_tag="ACG8M6_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0456161.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23464..23748)
                     /locus_tag="ACG8M6_000465"
     CDS             complement(23464..23748)
                     /locus_tag="ACG8M6_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456162.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23826..24020)
                     /gene="ssrS"
                     /locus_tag="ACG8M6_000466"
     ncRNA           complement(23826..24020)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8M6_000466"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24092..25378)
                     /locus_tag="ACG8M6_000467"
     CDS             complement(24092..25378)
                     /locus_tag="ACG8M6_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0456163.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25516..26187
                     /locus_tag="ACG8M6_000468"
     CDS             25516..26187
                     /locus_tag="ACG8M6_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0456164.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26153..26641
                     /locus_tag="ACG8M6_000469"
     CDS             26153..26641
                     /locus_tag="ACG8M6_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0456165.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26654..27601
                     /gene="prmA"
                     /locus_tag="ACG8M6_000470"
     CDS             26654..27601
                     /gene="prmA"
                     /locus_tag="ACG8M6_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0456166.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27602..28366
                     /locus_tag="ACG8M6_000471"
     CDS             27602..28366
                     /locus_tag="ACG8M6_000471"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0456167.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28599..30812
                     /locus_tag="ACG8M6_000472"
     CDS             28599..30812
                     /locus_tag="ACG8M6_000472"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0456168.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30823..31269
                     /gene="dtd"
                     /locus_tag="ACG8M6_000473"
     CDS             30823..31269
                     /gene="dtd"
                     /locus_tag="ACG8M6_000473"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0456169.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31440..32699
                     /locus_tag="ACG8M6_000474"
     CDS             31440..32699
                     /locus_tag="ACG8M6_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0456170.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32788..33735)
                     /locus_tag="ACG8M6_000475"
     CDS             complement(32788..33735)
                     /locus_tag="ACG8M6_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0456171.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34129..35421
                     /gene="hisS"
                     /locus_tag="ACG8M6_000476"
     CDS             34129..35421
                     /gene="hisS"
                     /locus_tag="ACG8M6_000476"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0456172.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35436..37205
                     /gene="aspS"
                     /locus_tag="ACG8M6_000477"
     CDS             35436..37205
                     /gene="aspS"
                     /locus_tag="ACG8M6_000477"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0456173.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37314..38189
                     /locus_tag="ACG8M6_000478"
     CDS             37314..38189
                     /locus_tag="ACG8M6_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0456174.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38455..40338
                     /locus_tag="ACG8M6_000479"
     CDS             38455..40338
                     /locus_tag="ACG8M6_000479"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0456175.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40413..40697)
                     /locus_tag="ACG8M6_000480"
     CDS             complement(40413..40697)
                     /locus_tag="ACG8M6_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0456176.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40687..41421)
                     /locus_tag="ACG8M6_000481"
     CDS             complement(40687..41421)
                     /locus_tag="ACG8M6_000481"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0456177.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41550..42173
                     /locus_tag="ACG8M6_000482"
     CDS             41550..42173
                     /locus_tag="ACG8M6_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0456178.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42365..43486
                     /locus_tag="ACG8M6_000483"
     CDS             42365..43486
                     /locus_tag="ACG8M6_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0456179.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43493..46621
                     /locus_tag="ACG8M6_000484"
     CDS             43493..46621
                     /locus_tag="ACG8M6_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0456180.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46640..49834
                     /locus_tag="ACG8M6_000485"
     CDS             46640..49834
                     /locus_tag="ACG8M6_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0456181.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49980..50687)
                     /locus_tag="ACG8M6_000486"
     CDS             complement(49980..50687)
                     /locus_tag="ACG8M6_000486"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0456182.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16653 a         8915 c        10595 g        14738 t
ORIGIN      
        1 atatgttttg tgagaaattt agacttacaa taagcagtga atttataatc aacgtatagg
       61 agacatggat gaaatgaaac taattggtat agatttagac ggtactcttt tgaattctga
      121 ccagagcatt agtcaaaaaa atgcaagaac aatgaaacat ttaccggaag actgttttcc
      181 attcatttgt tctggaagag aagtagaaga cattaataat attctcaaaa aatctggttt
      241 aaggatacca gcagtagggt taaatggttc tgttggattt gatggagaaa aaaagatatt
      301 tgagtcttcc ttcgataaaa atgatgtaaa aaaaattgat tctgtcctat cgaaatttcc
      361 aaccaaaata tatacaaatc tcggaagtta tgaatctcag aattataaaa ataaaatgaa
      421 aaaaattttt cacgaggtag ggagtgaatt ttctatcaaa gagctaaact atgagcttga
      481 gtatgaaaaa tctatttgtt caatccctta taataatatt gaggaaatta tagaaaagga
      541 tgctatcagc atttacaaat tttttatttt tattcctaat cgtcaattga aagctaatat
      601 atttaaccat ttgaaaaaga tgctaaatgt gtctgttact gaatcggcac ctgtaaatat
      661 tgaaatcgta cctcaaaatg tatctaaagg tgtggtgttt gatcgtttgg aatcaatcta
      721 caatttaaag aaacctttga gaatcgcgat cggagatagt ttaaatgact tatctatgtt
      781 tgaaagtgca gatattagtt ttgcaatggg aaatagtcat caagaaatca tgaaaaaagc
      841 tgattatgtc actgtttcaa atgataaaga cggagtatct caagcatttg aaagaattct
      901 taccatataa actaattaag gtagtataga acatagaatt ctaaatacgg aaaaatggag
      961 cataaattcc agtacaaata gtacttcttc agtgtcaaac gaagcgaata taacatagat
     1021 atttaggatt gtgacaaaaa tcatgtcaca atcttttttt cgaataaact tattttttga
     1081 agtcggccat ttatcgtaat ttctttgcat aatacgaaca aattttcggt tattgatgaa
     1141 aaaaaacatt gaaaatggta tgataaaaga aaaaaaggag gaaattttta atggtgaaat
     1201 ttatccatgc ggcagattta catatggatc ggtcatttga aggactgaca actcttgata
     1261 aaactgtaca ggaaaaacta ttgaagacga atctgaccgt tttatcaaat atcattgaac
     1321 aggcaataat aaataacgtt gattttgttt tacttgcagg agataatttt catcaaaatc
     1381 gtccttcatt aaaaatacag aaacattttt cagaacagat gaaacggtta gaaaagaacc
     1441 atatacctgt atttataata ttcggaaatc atgatttcta tcaaaaagag cggtattggt
     1501 tctcctttcc taaaaatgtc catttattta catccgaaac ggtagagaca aaaaaaataa
     1561 cgataaaaag tggagaaact gtctctctca gtggatttag ttatcgtcaa ccgtggattc
     1621 aaaaagacaa agtgatggaa tttccttcta gagaactcac agattaccat attgggttgt
     1681 accacgggga acctggcgtc tctcaaaaag gaaactatgc tcctttccaa ccctcaaaga
     1741 tgcaagaaaa gggatacgat tattgggctt tagggcatat ccatgtgcca acggtcttaa
     1801 atgaaaagca aacaattgtt tatccaggtg cgcctcaagg tcatactaaa aaggaacaag
     1861 cttctacatc tattctacta gttgaattaa gtaaaggaag ttgccaaata catccgatta
     1921 aagtagcaga ggtctattgg aagacaaagg aaatatcatt gaggaaagca agaacgacaa
     1981 aagaggtcat tgttcacatt cgtcagcagc tttcaaaagt agaggatgga tttagtttaa
     2041 ttgagataaa attaaaagat tatgaccatc tcaatacaga tgttttggaa cggatacaat
     2101 caggagagct attagactat ttgttagagg aattctcaga taggataaat gatttcttta
     2161 tttggcgtat ttctctcata gagaatactt tttatacgaa gaccccactt gctgcttctg
     2221 ctaaattgat ggaacagtta tttcaatact atcagacacc tcaagatttc caacaaattt
     2281 taaatgaagt atatagtcat caagaagcgg ctagaatatt gagcgaactg ccggaatatc
     2341 aagaagaaac acttgaaaaa gccaagcagc tgcttaatca agatttttta tttgaggagg
     2401 atcaggaatg aagattttaa ggatcgagat tgctgctttt ggaaaatggc gtcaaaaaag
     2461 ttttgacttt tattcaggca atcagctgat ctatggggga aacgaagcag gaaaatcaac
     2521 gatttatcaa tttattcaag caatattatt tggtttccct tcaaaaggcc gtaaaaaaaa
     2581 ggattataca ccaaaagacg gctcagccta tggcggcaaa atatggctta aacatcctgt
     2641 atatggtgaa tttgctgtcg agcgctacaa gcagcaaaat cgcggaaaat cgaaggtgtg
     2701 gctgggggat caagtgggaa gtgatgagtt actagaaaaa ctgatcgcac ctttgacgaa
     2761 agagttattc cagcaggtat ttactttcca gcaagagcag ttgatggaac tagatcattt
     2821 gagagaaaaa gagctacatg atgcgctgat ttctttaggc atcacgggca gttctcaagt
     2881 attccaaaag cggcaagaat attatcagaa agcacaacaa ttattcaaga aaaaaggcca
     2941 aaaattacta atcaatcaaa aattaaatga atggaaagag ctgcaagaaa aaattcatca
     3001 aaaacaagaa caggaagctt attttggcaa gttgatccaa caagaacagg aatacgagtc
     3061 gcaacagcag cagttattgg cacaattaaa agaggcgaac aaacaatttt cattagccag
     3121 acagcaagaa cttaattttt ctttatatga agaatggcaa tctctcaaaa aaaacagcac
     3181 tacaggtctt cctgatgagc aacttctttt agatctggag tctttctcca aacggtatca
     3241 gcaaacaaat gagcgtttgg aagaattaga agcaacactg gaaaagcata gcgggatgga
     3301 tcagcagtca gccagatatt atttttattt agagcaggag caggaactaa aagatctcca
     3361 gcaacttcaa tttacagcgg aacaattaaa cgaagaatgg caaagattac aaattgagac
     3421 tagagaaaca aattcagaat tgaagatttt ggaacaaagg tggcattggc agaaaaatcc
     3481 gttaccggaa gaatttttat atgaagaaga atggaagcat cttctggctg aagaaagaag
     3541 gattgctgat ctagaaagcc agatttctgt gcgtctccaa atagctaaag aacagcaaga
     3601 gatgattgaa gcagaagtga atgacttgga agagacctat ccagcattat tacaaaatca
     3661 gtcagagata gcaccagtct cttcatctaa accgtatatg ctgatggctg gcggagcaat
     3721 aatcattgca ggtatatttg taccaatgcc cttgaaagtg atccttttac taattggtgc
     3781 aggcatgatt ggctatttct tctacaaaag gcaaccaaaa attaaaacta aaaatgattt
     3841 ctctgagata aaaggaatgt ggcaagaaaa actaggacaa cttgatctag ccttagctca
     3901 attagctgaa gcagaagaaa aacaaaagag aactggagaa gaaaaacaga ttcttcagca
     3961 agaagtatat cgttttgcgg atcatcggca cttaggaaag atggatacgc tcaaactcat
     4021 gaaagaatat ggacaggaag tcatagatta tcagaaatta tatggcgtat ttgagcggaa
     4081 acataaaaga gaacaggaaa tacaagaaaa actacgacaa atcgaccaaa aatttgagtt
     4141 tgttaaagaa tggttaccga tacaagataa gacaatttca gaaaaaatgg tattgctttc
     4201 ccgttttata caagagatgg aacagatgaa atttgccaga agctatcagc aaaatacttt
     4261 attgaagcaa gaaatcaatc aattgaaaaa gaaacaaaaa gtatggatac aagaatacag
     4321 cgaacaattg actcaagcag gcttatctta tccttcggaa gtacctgtct ttttacaaaa
     4381 agccaaacaa gccgaacagc agatgaaaag atacaatgaa ttatcggaaa tgcttactcc
     4441 actatttcct gaagagctga caaaagagaa attatctgta agactgcgcc aactgaaaga
     4501 aaagcaatcc caactccagc aagaagaaaa agaagcaagt gaaaatcggc aacgactcca
     4561 acttcaagta gaagagttgc aaagagacgg cacattggac gaactttatc aacaagctag
     4621 ccggcaaaaa gcaaaattac aggaattact ggaggaatgg ggagcacttg aaatcgccgg
     4681 gacattctta ggtgatcttt caacagagat gagtgaaaga caattacccc agttgttaag
     4741 agcagcaggt gattatttag gtattttaac aaaaggaaat tatcagaaga tattactgaa
     4801 aaatgataca ctgactttaa cagacggata tacagaattt cccatctatg agttatctac
     4861 aggtacgaaa gatcagctga ttatggcgat gagatttgct tacctctcat tagaagctaa
     4921 ccgttcgata tgcccgatca tcattgatga tggctggctt cattatgatc atcagcgaaa
     4981 gtatcaatta gctgaacttt tagctaaatt tggagaaaag tatcaaataa tttgtttttc
     5041 ttctgataaa gaaatggtaa gctattacca tgagatgaac caaatcgtaa aacaattaga
     5101 aggagcaaat aatgaaaaaa attcgtgatt tagtaatcga tgagcagttt gaacaatttg
     5161 tcctgatcaa aagtgcagat gtaagaatcg ctaaaaacgg aaaaaaattt attgctttta
     5221 ctttccaaga tacttcaggt actatcgatg gtaaatactg ggatgcttcg gaggaggaaa
     5281 tcaataaatt tacagccggc acagtcgttt ttctaaacgg taaaagagag gtttatcaag
     5341 gaaaccctca agtaaagatt cttcatatgc gcttaacaag ggtagatgag ccaggagagc
     5401 ctagtttata catggaaaga gcccctttga agaaagaaga aatggaagaa gaaatcaatc
     5461 aattgttgtt tgagatcacc aatgcgcatt ggaatcgtat tgttcgctat ctacttaata
     5521 aatatcaaaa acaatttttt gagtttccgg cagcaaaaag aaaccatcat gcatttgcca
     5581 gtggattagc ttatcatact ttgacaatgc ttcacttagg gaaatcaatc gccgatgaat
     5641 atcctgagct gaatcgagca ttgttgtatg ctggtatcat tttgcatgat ttaggaaagg
     5701 tgattgaact ttccggtcct atgtcgacag aatatacgct tgcaggaaat ttgatcggtc
     5761 atctggtatt gattgacgaa gaaataacta aagcctgtct tgcgttgaaa atcagcgaag
     5821 ctgacgaaga tgttctcgta ttacgtcata tggttttagc acaccatggc ttattagaat
     5881 atggctcacc tgtacgtcca agaataatgg aagcagaaat attacatcaa atcgacaata
     5941 tcgatgcttc gatgcagatg atgttgactt cgattcgtca aacagaacca ggaaaataca
     6001 ccgatcggat tttcggtatg gacaaccgaa gtttctatgt accaaaagat ttatagcaaa
     6061 aatttatttt tttcattcaa aacaaaaaaa gcgagacata agtcttcgct ttttttgttt
     6121 tcttttattt tgatgaatca gttgtttctt cagttgaaga ttcttttgtt gaatcagttg
     6181 attttgtgtc agaactcttt gttgaagctg tagtttcact tgagtctttc gtacttgatg
     6241 aagttgttgt gaatgcactc aacacatttt caaaggcatc atctttgatt tttacatttg
     6301 catcttttaa ttcttcaccg attactttag ttgtgaatgc attgtcactt agtttagttt
     6361 cagtagcgat atcttttagt tgatctttgt atttatccat gtcattgcct ttattttggt
     6421 ttttcaccat tttgacaacg tagtattctg tcgcataaga agtaggattt gttgtagtaa
     6481 taacatcgga gatctcaccg tctttcaatt tgaaagctgc ttcttttact tctgcaggaa
     6541 tagttgttgt cgttgagtcg aatttaactt taccgccatc ttcttttgtc tctgtatcag
     6601 ttgatttgtc tttggctaat ttagagaaat catctccatc atccgctgat tttttcacat
     6661 cttttgcttc atcttcactt gataatttga tgatttgtgc ttctacttct ggatggaaag
     6721 atttccaagc agttttcaaa tcgtcatctg taatatctac gtgagctttc aagccagctt
     6781 caaaagctaa attgttacga atatattctt tataagtatc tttcgtatat ccagcagctt
     6841 ctaattgtga ttcaaaagta tcacctaatg atttcgcttg tttgtcgtat tctgcatcca
     6901 cttgcttatc ggttacttta tcgccatatt tgttattgaa tactttataa atgatcatcc
     6961 gttgtactaa ggattggttc gatgattcaa gttttgcttc atcatagaaa tcagatacag
     7021 tgatcgttcc gcctttcatc gttgcgatat ctttattagt atcgcctgaa caagctgcta
     7081 gagttaaaac agccaacgca ctagtagctg ccaaaataat tgattttttt ttcattaaaa
     7141 tttgcactcc tgtaattagt tattattttt atatattaac tataccatat cccatttcct
     7201 ttaaaaacgt tgtcgtggta gtttcaaaca aaattcaaaa tttcatcaca ttcctaggaa
     7261 atacgcaaac ttttctcact tatatagtta aatctgttga gaaaataatc attattatat
     7321 ttgttcagga tactctggca gatcagcttt tagtttttct acttgttcat tgatttgtgt
     7381 gatacgtggt tcggcttgga atttaaaatc ttcgatatct tttttgattg cttcttggaa
     7441 agcaggaatc acagattcag ctgtttcttt tgtttcgatc aatgcgcttt taaaatgatt
     7501 caggctgtta tttaaatcat ttgtcagagt tgttgcttct tccaattctt cgatcagttt
     7561 ctttcttgtt tcattcccgc tcctgggcgc caataacaga cctccgacag tgccagcagc
     7621 agctccaaat aacagtcctt tgaaaaagtt tttaatcatc gcaggacacc tgctttacga
     7681 ttgtttcttg aatagctttt agttcttccg atccataact atcttgatga ttgacaaagt
     7741 gaatagaaaa atcatcctct ttgctgtagc gagggactaa atggatatgt gaatgaaaaa
     7801 cagattgata ggctagttca cggttgttat tcaagatatt aagacctttc atttctggaa
     7861 aagctttttc caatgcttga gcaatcttag gcacacgcga aaaaacctct cctgcaagtt
     7921 cgggatcata ttcaaaaata tctgctacat gacgtttagg cacaactaat gtgtgtcctt
     7981 tcgttgcttg agagatgtct aaaaaagcat agaccttgtc gtcttcatat attttatagc
     8041 ttgggatttc ttgattgatg attttgcaaa aaatacagtt ttccatataa aacatccttt
     8101 ctataaagaa taaatacttc agcttaactt taccataagt taaagctatt gtaaggaaaa
     8161 aaacaaaata ccgtcatttt cttcgtggta taataaataa aaataaatgg cggaggaaaa
     8221 agaatgagtt taattatcga tcatgtcaca ggggggtacg gacatctccc tgtgttaaaa
     8281 gatatcagtt tcgatgtaca gcctggagag atagtagggt tgattggact gaacggagca
     8341 gggaaaagta cgaccatcaa gcacattatc ggtctgctgg aagcttcaaa aggaacgata
     8401 gaaatcaatc atctatcctt aaagaaaaat acggaagaat atcgcaaaca aattgggtat
     8461 attccagaga ctccttcttt atatgaagaa ttgacattga gagaacatat cgaagtcact
     8521 gcgatggcct acgatatacc tatggatata gcaatgaaaa gagcagaaaa gttgttggag
     8581 acttttcggt tatctaatcg tcttgactgg ttcccagcta atttttcaaa gggaatgaaa
     8641 cagaaagtga tgatcttatg cgcctttttg atcgagcctt ctttatatat catcgacgaa
     8701 ccatttttag gcttggatcc actagctatc aatgcattgc ttgaattgat ggtagaaatg
     8761 aaaaaagaag gagccggtat tttgatgtct acgcatattt tagctactgc agaaaaatat
     8821 tgtgatcgtt ttgtggtctt acataacgga gagatacgag cagacggttc tcttgagatg
     8881 ttgagaaaag aatttcagct gccggattct tctttggacg aaatctatat tgctttaaca
     8941 aaggagcagg cacatgagta gtttttttca aaaacgttta gcaagacatc aaaagaaaat
     9001 gatgaaatat atgcgttatg tattgaacga tcattttgtt ttagtttgtc ttttcttgat
     9061 tggcggtgtg ggattatatt attctaattg gttgaaaacc ctgcagccac cgtttgcaat
     9121 tggagggctc ataatctctg tattttggat gctctgttta ttcgtgggta agatggctac
     9181 ttttgcagag gcagcagata ttgttttttt acttcctaaa gaaaaagaaa tgcaaaccta
     9241 tttggaacga gcttttcgct attcttgcat ttttccgttg atctttttga tattggcatg
     9301 cggattggcc atgccattag tggtggttag tacggagcaa tccttttcga tgtttttcgt
     9361 ctatctgatc atattatgga gtttgaaatt cagtcatctg caaatcaaga gagcagaatt
     9421 gtttcgacta gatcaacaag ttattcgtta ttggaaatta ggctggtttt tttcttctct
     9481 gatctttttg tttctatcat tttattggct aatatgggca ggaatagcag gtgcattagt
     9541 acaggcaagt ctatattact ggtttttgtg gaagcaacaa gaaaaaggat tggattggga
     9601 gaaaatggtg catgtagaag aacataggtt gcatcagatc tatcaattta tcaatttatt
     9661 cacagatgtt cctgaaatta cggcaaagat caaaagaaga cgctatttgg atcctatatt
     9721 gactcatatt aaaagagaat caaaaaacac ttatctttac ttgtttgcaa gaagattggc
     9781 aagaggttct gattttagtg gtctttattt ccgattagta attataggtg ggttattgat
     9841 tgccggagta gtcgactttt attttagtct gattatcggt ttcttatttt tatatttgat
     9901 cggtttccaa ctgttacctt tagcgaacca gttccgctat atggttctcg ttcagcttta
     9961 tccaatccat aagaatcaga agaagcaggc gattcagtat ctactcacat gggtattaat
    10021 tatcgcgtcg gttatttttg gtttgattgc tgcagtcgta ttagatgggc agcagcgatg
    10081 ggttccgcta gcggcttatt tgattgttac tatgctcttt gttaaactat atgtaccaac
    10141 tcgtttgaag aagatgtcag actgaaaaca aagctgtgta gggtaaaaat gagaatgaaa
    10201 aatagagcgt tgacagtaag aatgctgttc gttctatttt ttttgagaaa agctggtttt
    10261 ttgaatggca attaaatgaa aaagaacgta tatgtgcttg acgtgtagtg ttcaggcgac
    10321 tagaataata agtactgcaa aacaaagagt aagaggacgg gagaatgcat gatggagttt
    10381 cagttagacc aagagtggcg gttacgtccc atcaaaggcg acacaggaaa aacatacata
    10441 ggcatgcgtg atgaggacaa agtctttatt aaacgaaaca cgactccgat gttggctgct
    10501 ctttcaaggg aaggaattac accaaaactt gtttggacaa aacgaacggg gaatggcgat
    10561 actttgacag cacaagagtg gctggaaggt cgcttgctga ctcctaatga gattggtcaa
    10621 agaaacgacg tgattgatgt tttgtaccat ttacatcatt caaattctct taaaaccatg
    10681 ctgacccgta tcggtggtcg tgtcatgacg ccggagaaga tgctgcaaaa atatgaaaaa
    10741 gaattaccgg aagaactcca gaaaaatcaa tttctgcaaa ttgtccatcg ttttcttcgt
    10801 gagcagatcc cgtcaataga taaggaaaac ttatcagtag tacacggaga tgtaaaccat
    10861 cgtaattggt tagtatgtaa aaattattta tatctagtcg attgggattc aatcatgttt
    10921 gcagaccctg cggtggatat tgcaacgatt ttaggaaatt atgtgccact gtctagctgg
    10981 aatcagtggc tgatcagtta tggcatacgc ccgactgacg aaatgcttga aaaatttcat
    11041 tggtatgcgg ccatgaattt gctccaagaa atcaccagat attgcaaacg tgaagatcat
    11101 cgacggatga acgaagaaat tttacaatta aaacgaattt tcagtggctg aggtttggcc
    11161 caaactctgc ggagatttgg ccagaccttt ttaattagag gaaaggaatg gagataaata
    11221 atgcgtgtcc gaaatcgtcc gggagcagcg gaaatgttag cggctcaccc gaattttgta
    11281 attagtgatc caacattgtg gaagggaaaa tggaatgaat tgtttgaaaa cgatcatcca
    11341 atccacattg agattggtat ggggaaaggc cagttcatca ctggaatggc aaaagcccat
    11401 cctgaaatca attatattgg agtagaaatg caggtcagtg ttgtttcgat tgcactagac
    11461 aagttgatcg aacaaccgct tcctaacctg aaacttctac atgtggatgg atcagcgttg
    11521 acagagtatt tcgctgacag cgaagtagat caaatctatc tgaatttttc cgatccatgg
    11581 ccaaaaaaac gtcatgaaaa acggcgattg acatataaga cttttcttgc tgtagatgaa
    11641 cagattcttc gtccaaatgg agaaatccat ttcaaaacag ataatcaagg attgtttgag
    11701 tattctctgg caagtttttc tcaatatggc atgatcctca aacaggtatg gttagattta
    11761 catcaaagtc aatttgaagg caatattatg acggagtatg aagaaaaatt ttcttcaaaa
    11821 ggtcaacgta tttatcgagt agaagctcaa tttcaagata agaccaaaaa gaaaagtctg
    11881 tgaaactaac tttctgcttt cataaaaaca ttaatatcca taaataaatc ccagaaaatg
    11941 aacattacag atgtaatgta ttttctggga tttatttgat ttaaaatgta tttatttcat
    12001 cggtttttcc acaaggtgga atcaattgga agcgagaaaa tgaaaataat cctttgtcgg
    12061 tgatcttcaa ttcgggtatg acagataatg ttaagaaaga aagtgtcaaa aaaggatcaa
    12121 aggttttagc tgctcctagt tgatgtgctt tagttattaa ttcattcaac tgttcataga
    12181 cgatcgaaaa aggttcctgt gtcatgattc cgcctatagg aagagaaaca caagctattt
    12241 cctgctgttt gttgacaacg gtcatcccgc cacctttttg aatcaaaaga ttagcagcat
    12301 acagcatatc ctcgtcgttt gtcccaacga caactagatt atgcgagtca tgggcaatcg
    12361 ttgtcgctat cgctccttgt ttcagctgaa atcctttcac tatgcctaat ccaatgtttc
    12421 ccgtttgatg gtgacgctcg atcaccgcaa tctttaattg gtctttttcg attgaaggaa
    12481 tgaatcgttc gccttgtcgg tccacttttt ctaccaaatc tttagtcact agactattcg
    12541 ggataatttc gatcacatgg gcttgtgaag aagaaagaag cagctctaac gaatgtggat
    12601 ttaatggttg agcgtttagt tttggcagac aacacgtata tgaatttgaa tgatcagcaa
    12661 ataaatggtc ttggagttgc ccagattgaa caactaatgt tcccttatag aaaacttcat
    12721 gaattggcag cttggtaaga tcgtctgtta agaagaaatc agcttgatac ccaggggcaa
    12781 tcgcacctag tgttttcaaa tcaaaacatt ctgctgcatt caatgttgcc atctgaatgg
    12841 cttgtaatgg atcgattcca agtgcaatag cttgacggac gatatgatcg attgatcctt
    12901 cttttactaa atcattgatc agtttatcat cggtgacaaa gaagcatctt cttgaatttt
    12961 caggtgtgat cgccggtagt agcgatgcca gatcttttgc aacggtccct tctcgaacca
    13021 ttaaatacat accaagatcc aaacgttctt tagcttcttt tgcagttgta gcttcatgat
    13081 ccgtccgaat acctgctgca ggataaacat ttaactcttc catgccgata ccagctgcat
    13141 gcccgtcaat ttttttctta tgctggtgca tcaattggat cttttctagg atgtctgttt
    13201 cattatttgc tactgcttga tagttcatga cttcagctaa gcctaaaact tcgggttttt
    13261 gtaaaaaagg ggctaatttt tttgcattta aatttgcacc attggtctca aaaggggtta
    13321 caggtacaca agaaggtagc ataaaaaaga tattcatgag tgtttgtctt gcatcttcta
    13381 tcataaactc aagacctgta ctcccagcaa tgtttcctat ttcatgggga tctgtcacta
    13441 cagtagtaac tccatgaagc aaggatactt ttgaaaattc gcttggtgat aaaagtgagc
    13501 tttcgatatg cacatggcca tcgatgaatc ctggcactag atattgccca ttagcattat
    13561 gtacgctttt tccttcgtat tcaccgattc ctacgatcat tccttcgcag attgctacat
    13621 ctttttccat gatttctttc gtaaagacgt tgacgatctt agcatgcttg atcactaggt
    13681 cagcaggctt ttttttactt gctacttcga tatatttcct taatgttgtt tccaatggag
    13741 tgcctcccaa gataaatttt tataagtata acataaattc ggtttttgcc aatatttttt
    13801 tgtaaattca ttttatttga aacaaaaaga tcaaaaagac gtatgttttt tgtggagggg
    13861 agaataatgg aaataaaacg attatcggat caaatgataa gtgaaagttt tgccaaacat
    13921 gcccaagacg tatatatatt gccagcttca gaagtatttt acatctattt tcgctatgga
    13981 aaaaaacgaa cattctggaa gaaagtctct tcggcagatg gacagcaact gatctcaaga
    14041 tttaagtatt tgggacagat ggatatcgga gaaaaaagaa aagctcagtt aggtgcattt
    14101 acttattctt tagaaaaaca ggaagttcgt ctgcggttgt cttctgtagg agattacctt
    14161 caacgagaga gtttagtgat tcggctatta tacggtactt ttgaaacacc ccatcgttgc
    14221 tttagttctc gagattttgc aactatttta cagaaaaccg tcagacgtgg attatacctt
    14281 ttcagcggac cagtcggatc aggcaagact tctctaatgt atcggttggc tttggaagaa
    14341 aaattgcaag tgattactat cgaagatcct gtagagatag aagaaccgtt ttttctgcag
    14401 ctacagatca atccgaaaat agcacagact tatgatacac ttttgagact cgcattacgg
    14461 catcgtccag acttgctaat cattggcgag ataagagatg aatcaactgc acatgctgcg
    14521 gtaagagcag cactaacagg gcaccgggtt tttgcaaccg tacatgcacg caatcttgaa
    14581 ggtacattgg tcagaatcca tgaactgaca aagaaaaaag aagagctatc tgaatgttta
    14641 gctggtgtca tttatcaaga acttttggaa gatgccaatc accatccttc tgttttatgg
    14701 agttatcaat ttatggagga agaatcagta aaaaaatcat gggaggaaag ttatgaagaa
    14761 gctcaggaaa atatttggca gtcagaacta ttctaaagga ttaacaagaa gccagcaaaa
    14821 ccagtttgca caaaccatgg gagaactgct tttatctggt tttagtctgc aagaatccct
    14881 ttatttatta ctcacgatcc gttcgtttcc acctgttatt ttggcaaata tccaacatca
    14941 gctaaaggaa gggcaagcgt ttagtgaggt actttcgcaa ataggatatg gagcagagca
    15001 actggtacag atcgagttag ctgaacgtca tgggaatctg ccgacaactt tgttggaaat
    15061 tgccaaacag tttcggcttg ttgaaggatt tcgcaatgat ttgaaaaagc tgcttgcata
    15121 tccgatgcta ttgctcacct ttttgctagg tatcttgttt agtatgcggt tgttcgtttt
    15181 gcctcagctt attggttcag gaatggtcat gcaagagcat tggggtattc agctgataca
    15241 agtctttcat tggtatgttc tactattcat cctagcggtt actctattgg ctttactagt
    15301 aaggtatcgt ctgatgaagt tgtctcctgt tgatcagagt gaatggataa gcagacaatt
    15361 attgattgga ccgatatatt ccttgtatca atcggcttat ttgtcactag agattggaaa
    15421 gctttttcat gaaggtttcg aattaaggca aatcatagtc tgcttaaagg aaacgaaaca
    15481 agaaagtctg attcagcatc ttgcctctaa gttgattcaa ggattggagg caggtatgtc
    15541 attggctgag cagttccaga attatcgttt tctatcagaa gaattcagta aaattgtttt
    15601 acagggagag gcaaagggaa gtttaggtaa agaactgatt ttttacagcg aatggacaag
    15661 aaaacagctt ttcaaacgtc ttcatcactg gatgcatttt attcagccaa tcattttttt
    15721 gggcgtagcg gctttgattt tattagttta tgccgctgtt ttattacctg tttacggaag
    15781 catagaggag gttttgccat gaaaaagaga aaaatatctt attatagcgg ttttacattg
    15841 atcgaaatgc taatcgtctt actgatcatc agtgtattag tgttgttgtt cgtccctaat
    15901 ttgtcgcgtt ataggaatca tgtagaccaa gaaagtcgtg aagcgatcat ccagttggta
    15961 gacacacaaa aagagctcta tgcgcttcaa aataatggaa gagttcctac tgtggaggaa
    16021 ttgttgaatg aaggctatat caaacgagaa catgcagaaa tctatcagcg gccttgaagg
    16081 taaggtttta gcatcaggat atacgttgtt tgaatcactc ttactgattt tattacttac
    16141 aacgatcctt tcttttccag ttctcgcatt ttctgcatgg aagaatgaat tggcagtcta
    16201 tcaatttttc ggccaatttg aaaaacgaat ctattctacg caaaaaatcg ctattgtgaa
    16261 tcagatccaa acaggctttt attggaatag tgaagagaat caaatcattt ttcatgttcc
    16321 gaatccagat aaaataaatt gggaagtatt agatattcct gatgagatta ccttgaaacg
    16381 ccatgcttct attacttttg ctgctagaac cgggaatgaa agttcactaa aagcttatca
    16441 attttattgg gaagaaaaag agcggacgat tacgtatcaa ttccagatag ggagtggtcg
    16501 ctatacaaaa agaattgaat aaaacaggat ttgttttgtt ggagtccctt acagcgctta
    16561 tgatcgcttc tgtctgtgtc tacttagtga gctttggcca gctgtttttg cttcaggcag
    16621 aaaagaaaag tggggaggaa ctgctattta ttcgaatgat ttatgaagat atcaagacgc
    16681 agcggatgta tcacaaaaga ccagagcgaa gggaagaaat gatcccatac gatatccaaa
    16741 ttgtcgataa taatggagta atcgaaaaag tgtttgtcaa tatggagaaa gaagcgatca
    16801 gtattgaaaa aacaagtaat tccagcattt acgctgattg aatgtctaat tggactgatg
    16861 atattgagca gtctattgct cacattcaat cttttgattc agcaaaccat ccaagtaagt
    16921 gaatttttgc agcgtaaaga tcaaaaagag tggctaatat ttttagcaca attagaagaa
    16981 gaactcaaga gcagtcacaa tgtctctgtg aaaaatcagc aactgcacta cacgatcgag
    17041 gataaacagt atatcatcga aagatatcag gcaatgatac gtaaaagaag aacttccggc
    17101 ggacatcaac ctatgttaac aagtatcact gagcttcaat tattggaaaa agaacacaca
    17161 atttcttttt atgtacactt tgaaaatgga gaagatggat atgccatatg gacaaaaaac
    17221 gatcagtaga aaatgttata agggctctat tttattttct gtcttgcttt tatttctttt
    17281 atgtacgttt ttattgatgg cagcattaga aagctaccgt ttgtcaatgg atcttaatat
    17341 acgaatcaaa aattattata tagcaaaaat aatgaagcag atggctttat cggaacagtc
    17401 tacgcttgcc gaaagttcgg aaggagtttt ttactacaca actggatctg taacatatca
    17461 gtgggtacaa cagtccctat tcttagaggt ggaagtctca ccgtttatct ttcggttcac
    17521 agaagaagta aaaaatgata cagatactag tacagaatga tggaatgttc gtgaaaacgc
    17581 cctcttttct gttataacac tgtttttaat aaaaaaatgt ttgccgtttc acaaataaaa
    17641 aacagtgaaa acgattgaaa ttttgtcaat tgttcgatag aattaaacag gtgaaaaaat
    17701 tgttataata tatctagaaa tgaggtgcac catgttacca gaaaaaatgg aacaagggtt
    17761 taatcaaaac ctggaagcta ttcagctttt acagaatgca ttgggaactt cctttttaga
    17821 agcctatgtc gaaaatgcag aaaatttaat agatgattat caagtacgtg tggtggatgg
    17881 agtgccgaca aaagagacaa cgcaacggat cacagctctt tatgaagagt tgaagaaact
    17941 ttctttcgag ccagaagaat ggcgcagact gtctcaatta cttttattga agggcagcca
    18001 gacagaacat ttgcaggcga accatcaact cactcctgat agtatcggtt ttttatttgt
    18061 ttttcttatt gaacaactat acacaaataa aaaagcaccg gttaagattc tggatattgc
    18121 ggcaggaatg ggcaacttgt tattaacagt tcttttaaac ctatcgaatg cggggtacca
    18181 aacagaagga attggtgtgg atattgatga tacattgttg gctgtcgcag cttctacaag
    18241 tgatttgaca caagcaaatg ttcaatactt tcatcaagac gggttgcagg aattattaat
    18301 agatcctgtc gattttgcaa tcagcgattt gccaatcggt tattatccga atgacgaaaa
    18361 agcaaaagag tttttaacaa gtaccgaaga aggccatagt tatgcacatc atttattgct
    18421 agagcaatca atgaaatatg tgaagccgga tggttttggt ttgtttttga tgccgagtgg
    18481 atttttagaa acggatcaaa gtgaagaaat caaaaaatgg tttaaagaag aaggttatct
    18541 tcaaggaatg attcaattgc cagatgaatt gttccgtaat aaacaatcgc aaaaaagtat
    18601 tttgatctta cagaaaaaag gaccacaagc acaacaagta aaagaagtct tgcttgtgaa
    18661 acttgcttca ttaaaagaac cagagaaggt gacggaattt ttcaacgagt ttaaaaactg
    18721 gaaatcatct gctctgtaat cattcaacga gaaggagagt catatgtcta aaacaattgc
    18781 aatcaatgct gggagttcaa gtttgaaatg gcaattatat caaatgccag aagaaaccgt
    18841 agttgcgaaa gggatcgtcg aacgtatcgg tttacaagat tctattttta ccatcaagta
    18901 cggcgataat caaaaattcg aacaaatcat ggatatcgaa aaccacgaag tagcagtaaa
    18961 aatgctgtta gatcagttga ttgaactgca aattttaagc tcttatgatg aaatcaccgg
    19021 agtagggcac cgtgttgttc acggaggaga aacttatggg gattcagtag tgatcgatga
    19081 gaaagtaatg gaccgcattg ctgaattagc agaatttgct cctttgcata atcctgcaaa
    19141 cttaatgggg atcaaagcgt ttaaaaagat tttacctgac attttgagtg tggctgtatt
    19201 cgatacatct ttccactcaa caatgccaaa gcaaaattat ttatatagct taccggtgtc
    19261 ttattatgaa gatttcaaag taagaaaata tggcttccat ggcacaagtc atcgttatgt
    19321 gtcggaacgt gcggcaaaat tattgaacaa accgatcgaa gaactaaaaa tcatcacttg
    19381 ccacttagga aatggtgtat cgatcacagc agtcgatgga ggtaaatcca tggatacttc
    19441 tatgggattc acaccattag ctggggtgac aatgggtaca cgttcaggtg atatcgatcc
    19501 agctgttctc ccatatttaa tggaaaaatt agagattgat attgatgaaa tgatcaatgt
    19561 tttgaacaag aaatctggtc tgctaggcct aacaggattg tctagtgata tgcgtgatct
    19621 tgaaaagaac tatgaaaaag aacatattcg tttagcttat gacattttcg tggaccgcat
    19681 ccgaaaatac atcggcggtt atgtgacaac gatgaatggt gttgatgcaa ttgtctttac
    19741 tgctggtatc ggcgagaatg acggacatgt acgtagtgaa atcatcaaag gtatgtcttg
    19801 gtttggttgt gaaatcgatc cagcgttgaa tgaattgcgt ggtgaagaag tagatatctc
    19861 tacaaaagaa tcaaaagtaa gagtattagt gatcccaaca gatgaggaac taatgattgc
    19921 ccgtgatgtg gagcgtctgc gcggctaagc caaagcattc gttaggataa aagatgaaac
    19981 gcatgcaaga gggaattctt gcatgtgttt tttttgcgga gaagaccgca aaatggtaat
    20041 ttaacttcaa tttcccttct tttttacagg atcttcttag ggaattttgc ttggaaaagt
    20101 ggtcaaaagg gaaaagactg gcattttatt tattgatttt caggaaaatc ccaatatttt
    20161 attttcattc atctttataa gggacattac atatgtaaaa agaaaaaaca tttttagatc
    20221 agcctcgtgg gctgtcgact ttattcttcg ctttatgttt agctagaaaa ccgatccgaa
    20281 acattcattg atcgaataat aaaaacttga ttttccacct gtactttgcc tgaatcgtga
    20341 aagcacaggt atgaaaatca agttttttgt tgcgaaaaaa cgtcctagtc aatcagttgg
    20401 ccgagcggct ggcggctgat ctgctcctga tcaataacga gtgcttcttg gaagtgttgc
    20461 tgcaaaaagt agcggtcatg gctgactgct aggagcgttc cttgatagtc ttgcagcatt
    20521 tcttcaattt cttctcgagc atagctgtcg aggtgattgg ttggttcatc caaaatcagg
    20581 acgttgattt tttctaaaaa gagtttcagc aacgccaaac ggattttttc accaccggat
    20641 aaatctttga tccgtttacc cacatcgctt tgataaaagc cacttttggc taattgccgt
    20701 cgagcgactt cttcgtttcc agtcaatgtg atggcgtaag ccagcaaacg gctatcggga
    20761 tcggcaaact gcagctgctg cggcaaataa ccgatttgca agctactacc taatttgatg
    20821 gtgccaccgt aaggcttttc ctcaccgagt aataatttga gtaaggttga ttttcctgca
    20881 ccattgtcgc cgatgatggc taaatgatct ccacgataga ctgcaaaact gctgtctttg
    20941 aatagtactt gatcagcgta ctgctggtgc aagttttgaa tgatgaaaac ttctttgcca
    21001 gattttccac cattagacaa gctttgcagc cgatttttcg ggggaggtgg cggtttgacg
    21061 agtgtcagtt tcgccaggcg gcgttccagt tctttggctt ttttgaagaa ggactcattg
    21121 tctccttcat gtgcccactg acgaaaacga cgaatcatga ctttcaaccg ctggacttct
    21181 ttttgctgca gttcgtaatt ttttgtcagc tcagccaagc gagcttcttt caagactcga
    21241 aaacggctgt agtttccggg atactcaatc aatgcacctt cttccagttc ccatgttttt
    21301 gtggtgactt gatcgagaaa ttgccgatca tgagaaacga ccagaaccga tttttttgta
    21361 ttctgtaaat agttttccaa ccagcgaata ctgttaagat ctaaatgatt ggtcggttca
    21421 tcgagtaaaa gtaaatcggc atcggctgct aaaattttgg ctaaagcgac tttaactcgt
    21481 tcgcctccgc taagttctgt taaccgtgct tcctgacggt gtggaatccc taagccttgc
    21541 aagatcgctg caatccggtc ttctaatacg taaccgccta gttcttcaaa gcgatgctgc
    21601 agctcaccgt aacgggccag acacttctct aagtttgtat ctggttctgt catcaatgct
    21661 tcgtagcgac tcatttgctg tttgaggtcc tgtatctcag cggcagaatg gagcaagtag
    21721 tggttgacgg tcgcttcgga aactgtaagt tcttgttcga ccgtcccgat ctttaagcct
    21781 tttttgcgac tgataacacc gctgtcgaca ccttctctgc cggtcaagat ttgtaaaaag
    21841 gttgatttgc cagttccgtt gataccgact aaggcaattt tttcaccagc ttcaatttga
    21901 caggataaat cctcaaataa tggattcccg ccgattgttt ttttaataga aattgcttgt
    21961 atgatcatga tctctctcct tgagaccact caaaaaggcc atggttatcc atggcctgtg
    22021 ttatttaaac tcagattatc atgagaaaaa taccttgaga tggccattga ttttacgtga
    22081 attcaactgt tttggacaaa cttgtcccgt caacagcaac tgtaaaacca attgcatggc
    22141 taaatgcgat atggtatcgt tggtaatttt cattacttgt tattttagtc atgcgcatca
    22201 tagtattttc cctccttcgt gtagttgtct tttccagtgt acctgaaagt gtcagaaagt
    22261 tcaactagcg ctgacattta cttaatattt atcgatgtgt aattctgtca gagagctttt
    22321 tttatctgac tgagggtaat gtctcgtgga ccacatcatt gtattctgag ctaaaccagc
    22381 gttcaggcga aatcatatgg tgtttgataa aaaactttgt ctacatgact tccttttgcc
    22441 atttgcttaa taccgttgca ttaaacagct cttctctttg gaaatctcac caacatcgcg
    22501 ttcaggagta tcgttgtggt tgcaatggca agcggtagcc tttcatcaat tcttgaatga
    22561 tttaccgaat gtagcacttg ccttctcgcg gtacccgaac ccgaaataaa attttagttt
    22621 ggcgataaaa tcggtatccg ccatcgatac ttcatatttt ttcgtatcag attcatcgga
    22681 aacttccaca ttgacgaacc aataagcttt ggcatgctta ggtcgtttta aaccacacca
    22741 gggcgactaa tcgccctggt gttcactttt ttttatttta cttgaactca ttgactgttt
    22801 tccctttatt ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt tgtttttatc
    22861 acggtttttt gtgcaaaaaa acctgccgat agttggcagg tcgtctacgt gttaagcttt
    22921 ggtgtggtct ttagctggca attggttttc taaatcagtt cggacaacaa gcacatcgca
    22981 agctgcgttt ctaataacat attcagaaac agaaccaata aataaacgtt ctacagcatt
    23041 caatcctgtg gctcctaaca taatcaaatc tacttgatta tcttctggga tttctctggc
    23101 aataatttgt tttggtgagc catattctac gacagaggtt acattattca agccagcttt
    23161 cttcgcattg ctttcataat ctgccaatgt ttgctttgcc atttcagtag cttgttctgc
    23221 aagcattcca tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga catgtgctaa
    23281 tagaagttct ccgttatttc ggatagctac attgaccgcc tttttaaaag ctaattcagc
    23341 ttcatcagaa ccatctacgg caaccatgat ttttttgtat tgttgtaaca taagcattca
    23401 ctcctcaagt caatcgtatt gtactctttt attgtagaac actttaggga aaaagagaat
    23461 tatttactgc tttggttcag caattttttt tgcaaatcgg atactgaagg tggcagaaac
    23521 aataattacg atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa aagctgttaa
    23581 aatacccatt acgcctaaaa cagcatatgc agctccgtaa aattgtagga atccttgatt
    23641 tttatcattt ttgttgatca atacaaaaaa gatatcctgt ttattaaata aataatagct
    23701 aacgaataat aatactaaag tggcgataat cattaaaatc ctaatcatga acaaatcctc
    23761 caaaataagc aacaaataga aaaagcaatt tgccaagagc aaattgcttt attctttaaa
    23821 agatggtaat ctccgttaat gccgttgttt gtcctgtagt attgatcccg cgaattcaag
    23881 caggtgggtc ccatggtcgt agcttcgaac taccaaatga taaggcatgt taccagcagt
    23941 gacacaagac gggtcccaag atatcaataa aaatgttcgg tcaacacaca aagaggacaa
    24001 ttcgaacatc acagatttcc cacctctata gtagcatatg aatttaaaaa gagcaatgag
    24061 agagctcgca gaaacagaac cgttttcttg tctagttttt tggaggatgc tttttccact
    24121 cttttatacg gtaatactgt tgacccagag cctgttcata tttccctgta tcttttggct
    24181 gataatactg agcattcttc agtttatctg gtaaatattg ctgattgacc caagcctgat
    24241 caaaatgatg aggatactga taacccacgc cacggttgag tgatttcgca cctttgtaat
    24301 ggctatctct caaatgatca ggaacatctc cagctttgcc ctctcgaata tcagcaagtg
    24361 ctgcatctaa tgccatgtag gcagaattgg attttggcga caagcaaaga tcgacgacga
    24421 cgtctgctag tgggatacgt gcctcaggca atcccaattt ttcagcagct aatacagcgt
    24481 tcactgtacg agccgcagca gcaggattac ctaaacctat atcttcataa ccgataacca
    24541 tcagacgccg gcaaatactt gccagatcac ctgcctcaac taaacgggcg agataatgaa
    24601 gtgcagcatc cacatcactt ccacgaatcg atttttgaaa agccgaaata acgtcataat
    24661 gagcatctcc atttttgtca tgggtaagag cttttctttg gacacattct tcaatgatcg
    24721 acagtgtaag atgaatcctt ccttctttat ctgaaggagt cgatagtgta gccagttcca
    24781 gcccattcaa tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga agaagagcat
    24841 cttcgtctaa ctggattgct tgttgtccaa ggccgcgttc tttatccctt aaagcatgtt
    24901 cgactgccaa ctggatgtct tgttctgtta atggtttgac ttcaaagatc tgtgtacggc
    24961 tgcggatcgc tggattgatg gtaatataag gattctcagt agtcgctccg atcaagatga
    25021 ttcggccact ttctaaatga ggaagcagaa aatcctgttt tgttttatct aatcggtgta
    25081 cttcatccaa taagaggatc actgtaccac tcatttttgc ttcttccgct acaacttgta
    25141 gatccttttt tgaatctgta gcggcgttta gcatgcgaaa tgcataatta gtagagccgg
    25201 cgatcgcact tgcgatactc gtttttccag taccaggagg tccatataga atcatggaag
    25261 agagcatgcg agcatcgacc atgcggcgta tgatttttcc ttccccgact agatgctgct
    25321 gaccaacgac ttcatcgatc gttcgaggtc tcatgcgata agccaatggc tgttgcatag
    25381 gattttcctc cttcttgttt ttacgtccct taattatatc atatttagca agatgcctag
    25441 aaagaacagc cttctgttgt tttgttttca tagcttatcc aactaatatt gtgtatgata
    25501 aagtaggtga gaaaaatgac ggataaacaa aagatagatg aatttttttg gaataaaacg
    25561 acgccagaag tggcacggga tttattgggg atgtatttgg agcatgacac accagaagga
    25621 agattagctg gatatattgt agacgcggaa gcatacctag gtccagaaga cgaggccgcc
    25681 catagctatg gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa accaggtacg
    25741 atctaccttt acacgatgca tactcatcgg attttgaaca tcattactca gccagaaggg
    25801 attcctcaag gagtaatgat acgtgcaatc gaacccgccg caatgattga tcaaatgagt
    25861 aaaaatcgtg gcggaaaaac tggtccagat atcagcaacg gtccgggaaa attagtggaa
    25921 gcattagcaa ttcctcaaga actttatggt cagtctatag cagatagttc gttacgctta
    25981 gtttttgaga aaaaaaagac accaaaaaag atcaatgctt tgcctagaat cggtatccca
    26041 aacaaaggtg tatggacaga aaagccgttg cgttttgttg tgtcgggaaa tccttatatc
    26101 tctttacaaa gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga aaatgaaaaa
    26161 gaaggatcta ttaacatatt tagatgaaat aattgaaaaa caagtgacag actatgatgt
    26221 agctttagat tgggatacaa aaaatcatac gatcgagatc gttgttcgct tgtttgcaga
    26281 aaataaagca caatttgaga ttgatgatgc agaagggatc gtttcgcaag aaccaattat
    26341 cgaatttgaa gacggcgtac ttctttttaa tcctcagaaa tctgtttttg atgaacgcaa
    26401 ttatctagca gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag tagctgattc
    26461 tttagtggaa tatctaaatg aagtattagc tcaagggcaa agcgatttgc ttgatttttt
    26521 ggatgaagac gatgacgaag ctgtattcga gcttcactgg gataatcagc aactagcaga
    26581 attagtagaa gtaaaacagc aaaatgaagc agatacttat ttaccgtacc caagttatta
    26641 aggagtgaca gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg aagcagtcga
    26701 agcagttgct aatattttga tggaggcagg ggcgagtggt gtcgcgattg aagattcttt
    26761 agatgttgaa cattttcagt cggatcaata tggtgagatt ttagataaaa atgattttac
    26821 atcgctgaaa gaaggcgcac tagtcatggc ttattttccg gaaacgactt tcttaccgga
    26881 aatcttgcct tttatcaaag atcgtatcac acaattacct gagtttggtc tagctattgg
    26941 aaaaaataaa gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag cctggaagaa
    27001 atactatcat ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct gggaaaacta
    27061 ccagacagaa gatcctctag aaaaaattat cacactagat cctggtatgg catttggcac
    27121 aggcactcat cccacgactt ccttgacatt gacagcacta gaaacagtct tgcgtggtgg
    27181 tgaaacattg ctagatgtgg gaactgggtc gggcgtgcta agtattgcag caaaatatct
    27241 tggtgcaaaa gatgtctatg cctttgattt agacgaagta gccgttcgtt cggcgaaaga
    27301 aaatatggat atgaatgaaa tagccaaaga tgtccatgta tcagctaacg atcttttgac
    27361 aggaatcgag attgaaagtg atgtaatcgt tgcgaacatt ttagcagata tcatcttatt
    27421 gatgatccca gatgcttggc gtttactgaa gcaaacaggc acgctgatcg tttcaggaat
    27481 cattgaagag aagaagcaaa tggttctgga tgccatgtta gaacaagggt ttatcgtgga
    27541 tcaaattttc cagcaaaaag actggtatgc aattattttg aaaaaaccag aggtggaata
    27601 gatgcagcgg tattttttaa aagaatcata tgaatcaaaa gactattttg ttttagaagg
    27661 ggaaaactat caccacattg tacgggttat gaggatggaa cccgaggatg aagttttttt
    27721 ggcattcaat gatcaaatag ccattgttgc agaaatcacg gagattactg aagaggctgt
    27781 ttatctgaaa gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt cgattaccat
    27841 agcgagtggc tatcctaaag gggaaaagct ggattggatc gttcagaaat ccactgaatt
    27901 aggagcgcaa gcttttcttg gtttcccggc atcagcctct gtcgttaaat gggatgcaaa
    27961 aaaacgtatg aaacgacaac aacggcttga aaagattgct caagaggcag cggaacaatc
    28021 ccatcggcag atcgtgccat cagtagagtt attggaaacg aaacaagatt tatttgagcg
    28081 gctagatgat tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc aaggcgaaaa
    28141 atcaaatgca gccaaaattc ttagtgaaac aaaaccagat agtcgaatct tagctgtctt
    28201 tggaccagaa ggtggattat cccgcgatga agtcgattat tttgtagaac acaaagggat
    28261 actttgtgga ttaggaccaa gaatccttcg tacagaaaca gcacctcttt atttattaag
    28321 tgctgctagc tatcaatggg aattaatgaa tccttctttc agctaacgtt cttcattaat
    28381 aaaagagtat cgacagaaag aattgaaaaa atagtcaaca atttgtataa tagaaacaat
    28441 tatttataca atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg aacatttccg
    28501 taaaaaaacg tataactgca cggattttgg gacaattatc aaacagatat ttgtcccgat
    28561 tcccgtttta ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa gaaattttaa
    28621 cgggacccgg tgtcataaag ctcgttagtc aatacatggg tcccgaacat gtagcatttg
    28681 ttgagaaagc atgcgaatac gcgactgctg cacatgacgg acagtttaga aaatcaggcg
    28741 aaccctacat tattcatcct atccaagtcg caggtatatt agcagattta aaaatggatc
    28801 cccatacagt ggctacaggc ttcttacatg atgttgttga agatacagaa atcactttag
    28861 aagatctgag agaagaattt ggcgatgacg ttgctatgtt agtggacggc gtaaccaaat
    28921 tagggaaaat aaaatataaa tcccacgaag agcagctagc agaaaaccac cgaaagatgc
    28981 tgctagcaat ggctcaagat ttacgagtta tcatggtcaa attagctgat agactacata
    29041 acatgcgtac gttgaagcac ctgcgagaag ataagcaaag aaggatcgct caggaaactt
    29101 tagaaatcta tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa tgggaattag
    29161 aagatacagc acttcgttat ctaaatccaa aacagtatta ccgtatcgtc cacttgatgc
    29221 agacgaagag agaagaacgt gaaaaatacg taagtggtac tgttgaagat attcgaatag
    29281 cgacagaaga gctggggatt tttgcagaaa tctatggacg gccaaaacac atttattcaa
    29341 tctatcgtaa aatgaaagat cagaaaaagc agttcaacga aatttatgac ttgctagcta
    29401 ttcgagtgat cgtagattca atcaaggatt gttatgctgt attaggagca atccacacaa
    29461 aatggaaacc aatgcccggc agatttaagg attatattgc catgccaaaa gctaatatgt
    29521 accaatcttt gcatactacc gtgatcggtc cggcaggaaa tccggtagaa attcaaatcc
    29581 gaacacagga aatgcatgaa atcgctgaat tcggggttgc tgcacactgg gcctataaag
    29641 aaggaaaaaa tgaaaaagta gaaccagatg gtatgacgaa acaattaagc tggttccatg
    29701 agatactcga acttcaagac gaaagctatg atgcttctga atttatggaa ggcgtaaaag
    29761 gagatatctt tagtgataaa gtctacgtct tcacaccaaa aggagacgtt actgagttac
    29821 caaaaggatc cggaccattg gactttgcat acagtatcca tacagatatc ggtaacaaaa
    29881 ccactggtgc aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg aaaaacggag
    29941 atatcattga gatcatgact tctccaaatt catttggccc aagtcgcgac tggttgaaat
    30001 tagttgctac tagcaaagca agaaataaga tcaaacgttt cttcaaagcc caagatcgag
    30061 aagaaaatgt gatcaaaggc cacgaatccg tggtcaaatg tattacagat ctaggattta
    30121 cgcctaaaga tattttgacg aagaacaaac tgcaagaagc actcgatcgt tttaattatc
    30181 aaacagaaga tgatctctat gcagctgtag ggtatggaga agttagcccc ttgacgatgg
    30241 ccaatcgtct gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa aagcaagaag
    30301 cagaagaaat catgaatcag ccgaaaaaag aacctgacaa aatgaaagta cgtcatgaag
    30361 gtggcgttgt cattcaaggg gtagaaaact tactaattcg tatcagtcgc tgctgtaatc
    30421 cgattcctgg tgatgatatc gttggttata tcactaaagg cagagggata tccattcatc
    30481 gtcgagattg tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt ttgattgaag
    30541 tcgaatggga agatacatcg aatacacgaa aagagtatga tgcagatttg gaaatttacg
    30601 gctataatcg ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg ctaacgaaaa
    30661 atctcaacag cgttgaagca cggacgaata aagataaaat ggcgacgatc catttgacgg
    30721 ttggtatcca gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa gcagtacctg
    30781 atgtctacag tgtacgccgg acgaatggat aggaggacag atatgagagc agttgttcaa
    30841 cgggtaagta aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat tggctacggc
    30901 ctacttgttt tattaggcat tcatgaaacc gatacgcaaa aagatgtgga ctatctggta
    30961 aaaaaaattg cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa tattagtgta
    31021 gaagatgtgg gaggacaaat tttaagtatc tctcaattca cactgtttgc ggatacgaaa
    31081 aaaggaaatc gaccaagctt tgtggaagcc gcacgaccag aaacagctat tcctttatac
    31141 gaatcattca atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg acaattcgga
    31201 gcagatatgt cggtttctct cgtcaatgat ggacctgtaa cgatcattat tgattctaaa
    31261 aacaaataaa gtaatctggt tttcactagt aaaaactatg aaacacgtcg cttaagttag
    31321 actgataaac tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct tttgtcagca
    31381 gtcggaaaaa aagttaagct taaattatac aagttatttt ttataggagg ttgctgaaga
    31441 tgaaagaagt accagtagta gatatcaaaa tcacagattt aaaaaaattg tatcaaaaag
    31501 gatacaacat gttggaggag ttacgccatg aagcggacgc tcccgtcgtc aaagcaaaga
    31561 ttttcaataa agaagctatc acgatctacg gatcatcagc agcaaaagta ttttatgatc
    31621 caaggaattt caaacgaaaa ggtgctatgc caaaattagt attgaagaca ctttttggtc
    31681 aaggtggagt acagactttg gatggagctg ctcatcacca tcggaaaaat atttttatgg
    31741 atctaatgac accggaaaga atggaagatt atcatcgtat cttagataag aatctgacac
    31801 aagcacttga agcacagcat ggtcaatttg aactatttga tttaagtaag atggttttct
    31861 ttacatctat ttgcgagtgg gcaggtatca atttatcagc gatttctaaa gatgaggtcg
    31921 aaaaattagc agaatatcaa atctctatga tcagcggaac gtttacttct ccgattgatc
    31981 acataaaagg tgtcgaaaat cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag
    32041 aagctcgaca aaatcctgtg gctggaaaag aaaatgtggc attatatgct tttgccaatg
    32101 ccacagattt agatggtcag cttttacctc tggaagtggc agctgttgaa ttattgaaca
    32161 tcatccgtcc aacggttgct ctaactgtgt gggcggcatt gatgggacat gcacttttca
    32221 gtagaccaga tttgtatcag caattaaaaa atgacttttc gacgttacaa gatccgttta
    32281 ttcaagaaat gagacgctat tatcctttct tcccaatgct acctgcgatt tcgctgaaag
    32341 aagtagaagt agatggttat cgtataccag aaggcagttg ggttatttta gatttatatg
    32401 gaacagacca tgatgaacga acagttgaag cgccagattc ctttatgatc aaacggtatg
    32461 tagggaaagc aaaagatatt tcctataaag aagaatatga gatgattgca caaggcgggg
    32521 gaaattttcg acaaatgcat cgctgtgcag gagaatggat cactcttcat agtctacgtg
    32581 tgttttccga tcagctagta aataagtttg agttctctgt acctgaacaa gattggacga
    32641 ttccattcaa tcagtttccg acttatccga atagccgagc gctactttac aagaattaga
    32701 ttagcataaa cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc ttttttcata
    32761 ttactataaa ttgataaggt caattgatca aaatccacga aatgcttcat caatggtttg
    32821 ataatcttca ttggataaat gcaaatctaa agcttggaca ttgtcagata cttgctctgg
    32881 acgcttagct ccaggtataa ctacagacac tcgtggattc ttcatgtacc aagctaaaac
    32941 aagttgcgca ggagtggctt ggtatctttt agcaatgggg cgaagtacat cgacagcggt
    33001 taaaatttct ctgaaacgtt ctccttggaa atctggattt ctttttcgag gatctccttc
    33061 accaaattgt ttttcctcac caagttcata ttttcctgtc agcaaaccgg aagcaagagg
    33121 aaaataggga acaaaactga ttttgttttt ttcaagataa gggaataatt ctttttcagc
    33181 ctgccgatgg atcaaactgt atttatcttc tactacatcg acatatccat ctgcattcgc
    33241 ttctttcaat tgttctaacg tgaagtttga cacaccgact gctcggattt ttcctgcttc
    33301 ttttaattcg tgcaatgtag caactgattc attttttggt gtgctttcat ccggaaagtg
    33361 gatataaaaa atgtcgatgt aatcagtttg cagacgtttt agtgcatctt ccaccgattg
    33421 tttgagaaac tcaggagaat tatcaaatgt tcgcccttta tttggtacat gagcagcttt
    33481 tgtagcgatg acgacacgtg agcgatcata ttctttttcc ttcagtacct cgccaatcag
    33541 ttcttcggaa cggccgtttc cgtaagcata agcggtatcg atcaaattga tcccgctatt
    33601 caaagctgta cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa aaagattatg
    33661 tcctccaacg gcatttgctc ctaatccaag ctgttctgcg tagacttgtg aatgtccgat
    33721 tcttatttct gccatagctg attcctcctt tttgtctagt ctaaaacaaa ataccaaaaa
    33781 gctgaactaa aacgtttttg tgaggggaaa tagtcagata tctcttgact ttgagactgg
    33841 tttttagtag gttataagta atatcgaatg tcaatgaaag agcgagtaag tttttgaaac
    33901 tttacaagag aggatcatcg aggctgaaaa tgatctaaag tttcattagc ctaaagacac
    33961 tctggagatg gacgagtgaa aagccgtccc gcgtacgagc gttattcgta agaggaaaga
    34021 gaaatctttc aaaattaggt ggtaccgtgt atgaatttca ttcacccttg ttcatgatga
    34081 acaagggtga tttttttatt tacatagcta agaaagggag agaaaactat gagtttccaa
    34141 agaccaaaag gaacaaatga tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa
    34201 gaaacagcac gtttattatt cagagattat cagtataatg aaatgcgtac accgattttt
    34261 gaacactatg aagtgatatc ccgaagtgta ggggatacaa cggatatcgt ttcaaaagag
    34321 atgtatgatt tttatgacaa aggggaacgc catgtgacgc ttcgaccaga aggtacagca
    34381 ccaatcgttc gttcttttgt tgagcataaa ttgttcggcc cagagtatgc gaaaccgttc
    34441 aaaacttttt atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg acggttacgt
    34501 caattccatc aaatcggagt agaagcattt ggatcagaaa accctgcaac tgatgtggag
    34561 agcatggtga tggcacttga tttcttcaaa cagttgggga tcacgcaaat ccggctagtc
    34621 atcaattcat taggtgataa agaaacgcgt caagcctatc gtcaagcatt gatcgactat
    34681 cttttacctt ttgaatcaga gctgagtgaa gactctaaac gccgccttca tgaaaatcca
    34741 ttacgcgtat tggacagtaa agacaaacga gatcaaaaat ttgtggcaga agctccatct
    34801 atcttagatt atctaagcga gcctgctcaa aaacattttg ataccgttgt ttccatgctg
    34861 gatgcattgg ctgttcctta tgaaatcgac agtaatatgg ttcgcggact agattattac
    34921 acacatacga tttttgaaat catgagtgac gcaccaaaaa tgggggcaca agcaacgatt
    34981 tgcgcaggcg gacgttatga caatctagtg gaagaactag gtggtccttc aacacctggc
    35041 ttcggatttg caatgggtat cgagcgatta ctcttgacta tggaggcaga ggaagtagtt
    35101 atccctgcat ttaatgagtt ggatgcttac gtggtcgcac tcggagacga aacaaatatc
    35161 gaagcattga aagtggtcca agcgattcga aactttggtt tttcagctga ccgcgacttc
    35221 atgaaccgta aagcaaaagc gcaatttaag acagcagata aagcgaatgc caaattagta
    35281 ttggtgatcg gtgaagatga gcttgctagt caaacgatta atgtcaaagc aatggcaagc
    35341 cgaaaagaaa aaagttttcc attagaagag atatatgaac attttgatga agtctatgat
    35401 gaaatgactt tattcgagta ggaggaaaaa aataaatggc acaaagaacg atctattgcg
    35461 gattagtttc cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg gtacaaaaaa
    35521 gacgagacct tgggggagta atctttatcg acctacgtga tcgtgaagga atcgttcaaa
    35581 tcgtctttaa tcctgaaatc aacaaagaag catgggaaat cgcggataaa tgtcgcagtg
    35641 aatacgtgat cgaagtgact ggggaagtta gaagacgcgg agaaaatgca gtaaatccta
    35701 aaatggccac tggtgaattc gaagtgattg caagtgaaat cgtgattttg aatcaagcaa
    35761 aaaccccgcc atttcaaatt gaagatgatc aagcaatcag tgacgaaata cgtttgaaat
    35821 atcgttattt ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta agaaatgcga
    35881 caacaaaagc gattcgtcat tatttagatg ataatgactt tctcgatatc gaaacacctt
    35941 acctagggaa atctacacca gaaggcgcaa gagactacct agttccttca cgtgtccatg
    36001 ctggacattt ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg ctgatgaatg
    36061 ctggattcga ccgttattat caaatcgttc gttgtttccg cgatgaagat ttacgtggag
    36121 accgtcagcc tgaatttacg caagtagata ttgaaacaac gttcttaaca gcagaagaaa
    36181 tccaaactta cacagaggga ttgattgcga aggtaatgaa agaagttcgc ggtatcgaag
    36241 tcacattgcc atttcctcgt atgacctatg atgaagcaat ggctcgctac ggcagcgaca
    36301 aaccggatac acgttttgat atggaattga tcgatctaag cgatacagtc aaagaagtag
    36361 aattcaaagt cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca ttgaatgcta
    36421 aaggcgctgc agatcgctac tcacgaaaag acatggatca gttaggtcaa tatgtaggac
    36481 aattcggagc taaggggctt gcatggctga aagtagaaga agacggattg aaaggtccaa
    36541 ttgctaaatt catgggagaa gcaacagaag caatcattaa agcaacagat gcaaaaccag
    36601 gtgacttgct gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca ttaggtgcta
    36661 tccggacacg tttaggaaaa gaattaggac taattgatga atcgaaattc aatttcttat
    36721 gggtaactga ttggccacaa tttgaatttt cagaagaaga aggtcgatat gtctctgctc
    36781 accatccatt cacgatgcca aaagaagaag atatcccgtt attggctgaa gaccctgcaa
    36841 aagtctatgc agaagcttat gatatcgtct tgaacggtta tgaattaggc ggaggttcta
    36901 tccgtattca tactcgtgag cttcaagaaa aagtactaga aactctaggc tttacaaaag
    36961 aacaaatgga agaacaattt ggtttcttgt taacagcttt agattacgga ttccctccac
    37021 atggcggtat tgcattagga ttggaccgtt ttgcaatgtt attagccgga gaagataata
    37081 ttcgtgaagt cattgccttt cctaaaaacg gaaaagcagc agatgtgatg agtgatgcac
    37141 caagtatggt ttcgccttta caattgttcg aattgaatat tgatgtaacg aatatagaag
    37201 aatagccgat aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac aaccttgatt
    37261 taattgaaaa acactttata atgaagccat aacatataaa gcggggcttt tccatgaaaa
    37321 aaacattcga aaattgggct tatcatgatt acacgaccaa gctttcggta tctatcgttt
    37381 atgcgttgat cgcatcagtc gcattgaatt tcttttatca gccaggacat atttattcaa
    37441 gtggtattac tggtctagct cagattttga cgactttgtc aaaatcactg ataggattcg
    37501 aagtaccggt atcaacaact ttgtatttac tgaatatacc tttgtttgta ttggcgtggt
    37561 taaaaatcgg gaagaagttt acaattttca cttttatcac agtcactttg acctcccttt
    37621 gtatccatat tttaccggaa acagtcctat ctaccgatcc aatcatctgt gcgttgtttg
    37681 ggggggctgt gatgggacta ggtattgggc tagcgttgag aaacggccta tcatcgggcg
    37741 gtctagatat cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt ggttctattt
    37801 cgatctactt caatgcattg atcgtttttg ttgccggaat tctatttggc tggcaatata
    37861 tgttttacag tacgttgtct attttcgtca gtggaaaagt aactgatgtc gtttatacta
    37921 aacaaaagaa gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta gtaaatgaaa
    37981 tccaaaactg tatgcgtaga gggatcacga tcatcaatga agcagaaggt gcttataagc
    38041 atgataagca gacagtattg atgacgatcg ttacacgata cgaactgcct ctacttagag
    38101 aagcaatgaa agcttctgat ccacaagctt ttgtaagtat tgcagaaaac gtccagatac
    38161 ttggtcgttt ctatgaggat ggcatttaga tcgagtttga caaaaattca tgaaatactg
    38221 ctgtagacag acgttcgttt gtctgcagtt tttttctacc atagtagaat cagcaaagaa
    38281 aaactgcttt tttatttgaa attttcttaa agttgaaaaa aagcatgttt atttttttaa
    38341 atttatggga tgatagaaaa aaatagtaga gaaaggacac gattctactt gctattttca
    38401 cttaataccc gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa
    38461 aaacaaaaaa ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta
    38521 gctgtggtgg gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg
    38581 ggcgttccga tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa
    38641 tgggaacaaa atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc
    38701 tatccggcac ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga
    38761 gggctgttaa acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg
    38821 tggcaaatca ataacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg
    38881 catatctggt ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta
    38941 ttgttgatgg tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc
    39001 ttcctctccg ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt
    39061 tattacggta ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt
    39121 tggccaagta cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc
    39181 gctggaggaa tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac
    39241 aatttcatct attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca
    39301 gtgacagcac accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg
    39361 attggcaaac gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa
    39421 gcaaaggtga ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg
    39481 attctaatca tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat
    39541 tatggtcgta cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc
    39601 aaaccatggg ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg
    39661 gcaccgaaaa acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt
    39721 aaaaaattag cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa
    39781 gcgatccttg ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa
    39841 ctaacggcat ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat
    39901 ccaaaagtag tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt
    39961 gaaaaattaa aggaacaaga tttgttgaaa gacactgttc tgattggact aggaacgaat
    40021 ggctcattct cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta
    40081 tactgggtca atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt
    40141 gaacagatga cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat
    40201 gaccatagtg actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat
    40261 tacattcact tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag
    40321 acaaacgtga cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg
    40381 aagttttttt atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt
    40441 gagtgtgtct gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct
    40501 gcttttgttg cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt
    40561 cttttttgaa gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta
    40621 gaaagatcgg ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa
    40681 taatgttcat ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt
    40741 gtaagtgatc aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc
    40801 aactaacaaa atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg
    40861 tgcaattcga tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac
    40921 catagtaaag cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa
    40981 cgtcgtatga atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt
    41041 tggcagccct ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac
    41101 gacagataag ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt
    41161 aagcgcgata ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat
    41221 agccgcctgg gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc
    41281 aacaatcttc ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga
    41341 aaaagaaaac actttggaac tttggataat cttgatatct tctgcgtata attggtcgat
    41401 tcgttcgtct tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta
    41461 tactattttt attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact
    41521 atgaccataa gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc
    41581 gagtactatt agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc
    41641 aagatgagaa ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg
    41701 cggtagctgc agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc
    41761 cagttctgcg atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag
    41821 gaccaagcgt cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca
    41881 tgtttcccag tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca
    41941 gaatgtctaa agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc
    42001 tgttcaagcg tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca
    42061 agaaaacgga taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca
    42121 aattggacaa agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata
    42181 agtttatgcg tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg
    42241 aaaacgaggg agagacgtca agtctgccct ctttttgcgt tccaatatgt aaaatagaag
    42301 acaggtgtca tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga
    42361 ttgaatgcgt tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga
    42421 cttgattgaa gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa
    42481 agtagaggca atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc
    42541 cgtagaatta tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt
    42601 tgctatatca ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag
    42661 ttattcgaac tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa
    42721 aaacgtacaa tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga
    42781 agatgacaat atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga
    42841 gctatttgat tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt
    42901 aaaatctgat tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact
    42961 acttacaccg tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca
    43021 ggataatgct cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa
    43081 aaaaggcgta tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga
    43141 acccctgcat gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt
    43201 ttatcagtcc gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat
    43261 cccgaatatg atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc
    43321 aaacggtcga gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca
    43381 aaaattagca gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga
    43441 aaaatggctc ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc
    43501 gaaaaaatta caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg
    43561 tttgacagaa gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat
    43621 ttttgatggg atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa
    43681 agagatgcgt tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga
    43741 acatcagcag atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa
    43801 aggaaacgga acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg
    43861 aaaagaaata aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat
    43921 gaatctcgat gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa
    43981 ttttttggtt tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca
    44041 atttttccaa cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt
    44101 ggatcattta gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga
    44161 ggaaccagca gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact
    44221 cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca
    44281 actagaaaat tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat
    44341 attaataaac aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg
    44401 atggattggg tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga
    44461 aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc
    44521 gatgactacg cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca
    44581 aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt
    44641 cgaatcctta cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga
    44701 agaattgaat gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat
    44761 tcttcaagaa aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca
    44821 gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg
    44881 gtcgctcgca cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag
    44941 tcagctattg aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca
    45001 gcaaattgct cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc
    45061 aacggaacac cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa
    45121 agtagaacaa caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc
    45181 caaaagtgaa gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat
    45241 catggcaacg aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa
    45301 ggcatttcct gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc
    45361 tttagataga cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca
    45421 acaagcagaa aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa
    45481 taatcagctc cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac
    45541 ggatagtaac tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc
    45601 gaatcaacta gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct
    45661 tcggacacag cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa
    45721 ggaacaagaa agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga
    45781 aatctgtgca tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc
    45841 ttatcatgaa gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct
    45901 aactgagaca ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat
    45961 gatcgaaata aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa
    46021 aatctacgat gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac
    46081 gcaattacag cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga
    46141 acggtatgtg cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca
    46201 acgtttaacg aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg
    46261 gaaaaccgga ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca
    46321 taccctgtct ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga
    46381 tgtgatccag acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt
    46441 tgggtcatta gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag
    46501 tgaaggaaga atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca
    46561 acaaataaga attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata
    46621 aaaagggaga ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg
    46681 tcaatacttg caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg
    46741 gcatgcagaa gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga
    46801 gatagattac tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc
    46861 tgtggaactt tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat
    46921 gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct
    46981 aaaaacacaa aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac
    47041 gattgagact aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg
    47101 ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc
    47161 ttacacagaa aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg
    47221 ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca
    47281 tcaattatta ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct
    47341 tctcccagta gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca
    47401 tctgcagata aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt
    47461 atcattcaaa gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga
    47521 ttttttcttt atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa
    47581 atatcaacaa acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga
    47641 agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc
    47701 agagatcggc gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt
    47761 cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt
    47821 ctatggtgaa gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt
    47881 tcaggtgtta cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg
    47941 ctaccagaaa aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc
    48001 ctttttccgc acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa
    48061 attgcgagag ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac
    48121 cggttcatgg ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca
    48181 tgtcctgcaa agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct
    48241 ttcttttgat tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc
    48301 gatacgtaca gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa
    48361 tcaattggaa aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct
    48421 cattcatcca gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga
    48481 ttatcaaaag caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat
    48541 tttagccgat gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga
    48601 aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa
    48661 ctggcaggca gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa
    48721 taaaaaagaa cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc
    48781 cagcttaaga caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga
    48841 tgaggcacaa atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc
    48901 tgtacctcag cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg
    48961 gtcattgttt gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact
    49021 tgccgcagaa gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac
    49081 tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga
    49141 acaaaaaact gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga
    49201 ttctgaagca ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca
    49261 aatttgctgc gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga
    49321 acaagtaaaa gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc
    49381 tcaatttaca gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac
    49441 attttattta agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa
    49501 tgctggcgaa aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct
    49561 cacgggagca gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca
    49621 agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat
    49681 ctctgaagga acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt
    49741 ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg
    49801 catgatttta agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg
    49861 ggtaagaaag cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc
    49921 tgggattcca ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt
    49981 tatacaaaga ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg
    50041 caaattggag tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta
    50101 gcactttttg cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct
    50161 ggaagcatat gaaattcttt ctcagccagc tgaaaaatct ctttttctgg tttttggaaa
    50221 cctgttgctt gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc
    50281 ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc
    50341 ttttccttca agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt
    50401 tcttcctcgt aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct
    50461 aaatctttta gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct
    50521 gccattactt ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc
    50581 atatctgcat ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc
    50641 tgatcataca tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc
    50701 tttcggtttt cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta
    50761 aaaaagctga taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt
    50821 ttttatattt cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca
    50881 aattaatcat tatggtagaa g
//
LOCUS       DAZKOB010000008        49976 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000008 DAZKOB010000000
VERSION     DAZKOB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.008"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..166)
                     /locus_tag="ACG8M6_000487"
     CDS             complement(<1..166)
                     /locus_tag="ACG8M6_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0456183.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLV"
     gene            complement(168..365)
                     /locus_tag="ACG8M6_000488"
     CDS             complement(168..365)
                     /locus_tag="ACG8M6_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0456184.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(759..2486)
                     /locus_tag="ACG8M6_000489"
     CDS             complement(759..2486)
                     /locus_tag="ACG8M6_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0456185.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2479..3672)
                     /locus_tag="ACG8M6_000490"
     CDS             complement(2479..3672)
                     /locus_tag="ACG8M6_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0456186.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3859..4503)
                     /locus_tag="ACG8M6_000491"
     CDS             complement(3859..4503)
                     /locus_tag="ACG8M6_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0456187.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4597..4731)
                     /locus_tag="ACG8M6_000492"
     CDS             complement(4597..4731)
                     /locus_tag="ACG8M6_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0456188.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4733..6865)
                     /gene="feoB"
                     /locus_tag="ACG8M6_000493"
     CDS             complement(4733..6865)
                     /gene="feoB"
                     /locus_tag="ACG8M6_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0456189.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6862..7335)
                     /locus_tag="ACG8M6_000494"
     CDS             complement(6862..7335)
                     /locus_tag="ACG8M6_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0456190.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7736..7960
                     /gene="nrdH"
                     /locus_tag="ACG8M6_000495"
     CDS             7736..7960
                     /gene="nrdH"
                     /locus_tag="ACG8M6_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0456191.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8119..10278
                     /gene="nrdE"
                     /locus_tag="ACG8M6_000496"
     CDS             8119..10278
                     /gene="nrdE"
                     /locus_tag="ACG8M6_000496"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0456192.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10328..11293
                     /gene="nrdF"
                     /locus_tag="ACG8M6_000497"
     CDS             10328..11293
                     /gene="nrdF"
                     /locus_tag="ACG8M6_000497"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0456193.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11382..12521)
                     /locus_tag="ACG8M6_000498"
     CDS             complement(11382..12521)
                     /locus_tag="ACG8M6_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0456194.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12830..13543)
                     /locus_tag="ACG8M6_000499"
     CDS             complement(12830..13543)
                     /locus_tag="ACG8M6_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0456195.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13797..14498)
                     /gene="nagB"
                     /locus_tag="ACG8M6_000500"
     CDS             complement(13797..14498)
                     /gene="nagB"
                     /locus_tag="ACG8M6_000500"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0456196.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14732..16264
                     /locus_tag="ACG8M6_000501"
     CDS             14732..16264
                     /locus_tag="ACG8M6_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0456197.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16455..17657)
                     /locus_tag="ACG8M6_000502"
     CDS             complement(16455..17657)
                     /locus_tag="ACG8M6_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0456198.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17739..18428)
                     /locus_tag="ACG8M6_000503"
     CDS             complement(17739..18428)
                     /locus_tag="ACG8M6_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0456199.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18534..18902)
                     /gene="rplL"
                     /locus_tag="ACG8M6_000504"
     CDS             complement(18534..18902)
                     /gene="rplL"
                     /locus_tag="ACG8M6_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0456200.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18955..19458)
                     /gene="rplJ"
                     /locus_tag="ACG8M6_000505"
     CDS             complement(18955..19458)
                     /gene="rplJ"
                     /locus_tag="ACG8M6_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0456201.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19662..19789)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19868..20557)
                     /gene="rplA"
                     /locus_tag="ACG8M6_000506"
     CDS             complement(19868..20557)
                     /gene="rplA"
                     /locus_tag="ACG8M6_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0456202.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20661..21083)
                     /gene="rplK"
                     /locus_tag="ACG8M6_000507"
     CDS             complement(20661..21083)
                     /gene="rplK"
                     /locus_tag="ACG8M6_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0456203.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21426..22094
                     /gene="sdaAB"
                     /locus_tag="ACG8M6_000508"
     CDS             21426..22094
                     /gene="sdaAB"
                     /locus_tag="ACG8M6_000508"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0456204.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22107..22979
                     /gene="sdaAA"
                     /locus_tag="ACG8M6_000509"
     CDS             22107..22979
                     /gene="sdaAA"
                     /locus_tag="ACG8M6_000509"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0456205.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23070..24170)
                     /locus_tag="ACG8M6_000510"
                     /pseudo
     CDS             complement(23070..24170)
                     /locus_tag="ACG8M6_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(24262..25149)
                     /locus_tag="ACG8M6_000511"
     CDS             complement(24262..25149)
                     /locus_tag="ACG8M6_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0456206.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25226..25771)
                     /gene="nusG"
                     /locus_tag="ACG8M6_000512"
     CDS             complement(25226..25771)
                     /gene="nusG"
                     /locus_tag="ACG8M6_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0456207.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25872..26042)
                     /gene="secE"
                     /locus_tag="ACG8M6_000513"
     CDS             complement(25872..26042)
                     /gene="secE"
                     /locus_tag="ACG8M6_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0456208.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26066..26218)
                     /gene="rpmG"
                     /locus_tag="ACG8M6_000514"
     CDS             complement(26066..26218)
                     /gene="rpmG"
                     /locus_tag="ACG8M6_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0456209.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26380..27024
                     /gene="cbpA"
                     /locus_tag="ACG8M6_000515"
     CDS             26380..27024
                     /gene="cbpA"
                     /locus_tag="ACG8M6_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0456210.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27080..27583)
                     /locus_tag="ACG8M6_000516"
     CDS             complement(27080..27583)
                     /locus_tag="ACG8M6_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0456211.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27596..27642)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28180..29145
                     /locus_tag="ACG8M6_000517"
     CDS             28180..29145
                     /locus_tag="ACG8M6_000517"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0456212.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29244..30416)
                     /locus_tag="ACG8M6_000518"
     CDS             complement(29244..30416)
                     /locus_tag="ACG8M6_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456213.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30403..31548)
                     /locus_tag="ACG8M6_000519"
     CDS             complement(30403..31548)
                     /locus_tag="ACG8M6_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456214.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31562..32486)
                     /locus_tag="ACG8M6_000520"
                     /pseudo
     CDS             complement(31562..32486)
                     /locus_tag="ACG8M6_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32603..33682
                     /locus_tag="ACG8M6_000521"
     CDS             32603..33682
                     /locus_tag="ACG8M6_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0456215.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33679..34307
                     /locus_tag="ACG8M6_000522"
                     /pseudo
     CDS             33679..34307
                     /locus_tag="ACG8M6_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(34417..34644)
                     /locus_tag="ACG8M6_000523"
     CDS             complement(34417..34644)
                     /locus_tag="ACG8M6_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0456216.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34747..37335)
                     /locus_tag="ACG8M6_000524"
     CDS             complement(34747..37335)
                     /locus_tag="ACG8M6_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0456217.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37355..38014)
                     /locus_tag="ACG8M6_000525"
     CDS             complement(37355..38014)
                     /locus_tag="ACG8M6_000525"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0456218.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38063..38566)
                     /gene="trmL"
                     /locus_tag="ACG8M6_000526"
     CDS             complement(38063..38566)
                     /gene="trmL"
                     /locus_tag="ACG8M6_000526"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0456219.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38787..39653)
                     /locus_tag="ACG8M6_000527"
     CDS             complement(38787..39653)
                     /locus_tag="ACG8M6_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0456220.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39762..40403
                     /locus_tag="ACG8M6_000528"
     CDS             39762..40403
                     /locus_tag="ACG8M6_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0456221.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40465..41826)
                     /gene="mgtE"
                     /locus_tag="ACG8M6_000529"
     CDS             complement(40465..41826)
                     /gene="mgtE"
                     /locus_tag="ACG8M6_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0456222.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41844..42743)
                     /locus_tag="ACG8M6_000530"
     CDS             complement(41844..42743)
                     /locus_tag="ACG8M6_000530"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0456223.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42745..43542)
                     /locus_tag="ACG8M6_000531"
     CDS             complement(42745..43542)
                     /locus_tag="ACG8M6_000531"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0456224.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43520..44224)
                     /locus_tag="ACG8M6_000532"
     CDS             complement(43520..44224)
                     /locus_tag="ACG8M6_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0456225.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44355..44931
                     /locus_tag="ACG8M6_000533"
                     /pseudo
     CDS             44355..44931
                     /locus_tag="ACG8M6_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            45049..45726
                     /locus_tag="ACG8M6_000534"
     CDS             45049..45726
                     /locus_tag="ACG8M6_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0456226.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45805..47613)
                     /gene="pepF"
                     /locus_tag="ACG8M6_000535"
     CDS             complement(45805..47613)
                     /gene="pepF"
                     /locus_tag="ACG8M6_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0456227.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47789..48787)
                     /locus_tag="ACG8M6_000536"
     CDS             complement(47789..48787)
                     /locus_tag="ACG8M6_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0456228.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49013..49666)
                     /locus_tag="ACG8M6_000537"
     CDS             complement(49013..49666)
                     /locus_tag="ACG8M6_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0456229.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14960 a        10488 c         8770 g        15758 t
ORIGIN      
        1 tcaccaatct atttattaaa ggaccgggat ctaattcata tttttgtttt tctaatttta
       61 aatatacctt ttgtaaaacc tcaagaatat cagaatattt aggatcttta cttatagata
      121 tatcattata taattcatga acaatttgct tagcattttt tttcatttta gcacttccct
      181 ggaattgctc cacctaaaaa accacctata gacattcctg caatacaagt agttgccttg
      241 gcccaatcga ccgtacattt atttttagtg caatacactc catttccata atattttcca
      301 ctatgagtgg tacccccata gataagttgt gtctctttaa tagacaaaat ttttaaatgt
      361 ttcataatct aatcacccct aaattaatgt ttttagacta ttattctaac attcattaaa
      421 tttcaaaaaa caattccttc ttgaaacgta gcaaaataat tcctgaataa gaatttaatc
      481 acttttaagt atataaacta tgtatattct ttaatatttt ttgagaatgc acttcgtaca
      541 taataaactt taatcacatt agtctttcat acctacgatc gatctctttg actcgcaccc
      601 ataaattcat aatagtttgt cggcgtcttt ggacgtttcg aagatgagct acgcattctc
      661 tttttttata gacaaaaaag atagagacga acatggagct tccaaatttg gatatttgat
      721 ccaattttgg aagctccatt tatttcgtgt ctgtcttttt attttttcca gctttcgact
      781 aattcttggt tctctttgat ccagtctttt gctgcttgct gtggatcttt tccattgttg
      841 atttccaaca taactttttc catatctttt tctgaccaat ggaaattgtc taatactttg
      901 taagcttctg gctggtcttc tttcaatcct tttcgaacca tcgtatggat ctgctcttcg
      961 cttcccattc cgttttcagg atcttctaag tatttcagat catatttggc aaacatccaa
     1021 tgaggtgtcc atcctgtaac tacaattggt tcgtgttttt tgatcgcttg acctagtgca
     1081 acggtcattg ctcctgaaga agatgtctct actttccagt ctttcaagtt gtcatatttt
     1141 tggatcgtgt tttcagcagc cgtaactaca ccagctcctg gctcgattcc tatgattttc
     1201 ttacctgcct gatcggttaa ctcatcgatc gaatttacat ccatgtaggc tggcactgct
     1261 aaaccaactt ttgctcctgt caaattggca cctaggtctt cgacttgatc tttatattgt
     1321 gcatattgtg aaccatgagt tttaggtagc caagctgaaa ccatggcatc tgactcgcca
     1381 tttgaaacag atttccacat gattgaatta tctaatggcg tcatcgttac atcatagccc
     1441 atttgtttca atacttcacc gactacattt gttgaagcaa cttctgtatc ccattcaaca
     1501 taagataagt tgatacgttt tgtttctttg gcagatgaaa aactccctat accaatcgca
     1561 cctaagatca ctactactac agcaccaatg atcagtcctt gacgtttttt acttttctgt
     1621 gttgttgctc ctgcagcttt tttattgttt ggctggttca aatgctgtgt aaaacggtca
     1681 atgatgattg ctaagataac taatgcaacc ccgttgacaa atccattccc gacttgtgct
     1741 ctttgcaatg ctgataatac gccgcgtcct aagccgggtg caccgatcat agaggcaata
     1801 acgaccattg aaagtgacag cattgttgtt tgattgatcc ctgccatgat cgtactttta
     1861 gctaaaggca attctagttt gaatagtttt tgacgacttg tgctcccgaa tgaatcagaa
     1921 gcttctacta attctttagg aacttgtcga atccctaaat tcgtaaaacg aacggttggt
     1981 ggcagagcaa agatgactga ggcaaatacc cctggaacca tcccgatacc gaaaaatgca
     2041 actgctggaa tcaaataaac gaatcctggc attgtctgca taaagtcaag gatcggtttg
     2101 ataatacttt gggctttact gctttttgcc atcaagatcc ctaatggtac cccaatgatg
     2161 atcgagatca cactagataa aagaacaagt gtgacagtat tcatcaaatc attccataga
     2221 ccttggttat aaataaacca tagaccgatt aatgtgaaca acgtaagtcc tggacgtttc
     2281 ttagaaataa agaaagctgc taccgtcaat aacaagataa acaaaggagc tggaatcact
     2341 aacagtaaat ttgtaatccc cgacatcact gcctgtccaa tcgtttgtaa aaatgaaaat
     2401 aggccagaaa aagttgtcgt aaaccattct gtgatatttt ctacccagtt tgccactggt
     2461 aattgatagt tcaatagatt attcatgttc actcacctcg cttgtttctg ctaatgcttc
     2521 aatcacacta cctcggatga cgacaccttt taattttcca tcctctacaa ctgcaagtgg
     2581 agtcggtgag tcataaataa tattgaaaat atcacttacc accatgtctt tcccaatcac
     2641 cgtcacattt tcatctagga agtcgatcaa tggacgtttt tgcttcctag cttctaacgc
     2701 ttgatctgca gtgatgatcc ctttcaggtg tctctttctg tcaacagcca tcagcatgct
     2761 gacttcttca ttacgcatcc ttgtcaatgc aacgtttggt ccatcagatt cgatattcgt
     2821 cgtcaatgca ggtaccatga tgttttgagc cgtaagcact ttggaacgat cgacttcttc
     2881 cacaaattca cgtacataat catttgctgg attggtcaat atttcttcac ctgttccgat
     2941 ctgcatgatt tcgccgtctt tcatcaaggc gattctgtcc ccaatccgta aagcttcatt
     3001 caaatcatgg gtaatgaaaa taatcgtttt ttgaacattg gcttgcaggt ccagtaattc
     3061 gtcttgcatt tctcgccgga tcagcggatc aagagccgag aacgcttcgt ccatcagcaa
     3121 gatctcagga tcatttgcta aagctctggc taggccaacc cgttgttgca tcccaccaga
     3181 taactgacta ggatattggt ctttaaatga aagcaaacta gaattttcta atgctttttc
     3241 tgctttttct tgtcgttctt ctttaggtac accacggact tctaacccgt attctgtatt
     3301 ttccaagatc gttcgatgtg gaaacagacc gaagttttgg aaaaccatat tgattttatg
     3361 acgacggact tctcgcaatt cttctttgct taacttcgat acatcttctc catcaatata
     3421 gatactgccc gaagttggct caatcagacg attcaataga cggatcaatg tggatttccc
     3481 actcccggaa agccccatga tcacgaagat ttctccttct tttacgtcaa aattcacgtc
     3541 atacacacca accgttgcac cggttttctt taaaatctcc gttttcggct ggtgatcatt
     3601 catcatatct aaagcggctt gtgtcttttt accaaatatc tttgttaaat gttttacttc
     3661 tacttttacc aaacaaattc tcctttttta tgtaaattca tttttctttc tgaaaaaagt
     3721 gaccacacta tgttaacatc atgtagtcac tttgtcaaaa atgaaagaaa tttgcttgtc
     3781 caaactgaaa aaaacttggc taaatctttg taaataaaag gtttagccaa gttaatattt
     3841 ttttaatatt tcacacaatc attctgcaag ctgatgctcc gggtaaaaaa agtttgtcat
     3901 acgctgtagt gtatattcat ttccaataaa atgaacgatg tcccctgccg taaatttagc
     3961 ataggggcca ggtgaaacaa gcagttcttc attatgagaa attgcgataa ctgtggcgcc
     4021 agtcgcctgc cagatattca actcgctaat tgaacggtcc agatgcattg ctttttctga
     4081 taaagataat tcaaacggaa gtaaaggatt catcttatgt attcgcttcg tttgattgac
     4141 cagctgatcc aataattgag aaaaatgatt cagctcatct tgttgcttct ccacacttgc
     4201 catgatttct gcttttgtat cttggatgga ttgtacatct tgatattgca ataggaaatc
     4261 ttttgctttt tccttcgaag caacaaatgc cccacttcca tgccgtactt ccatgattcc
     4321 aagatctacc agcacattga tagccttgcg ggtagtctca ggagaagcat tgtacgtact
     4381 ggcaagtgtt gagcgggcat ggatcttctc acccacttta tatcggtttt caacgatacg
     4441 ttcggctaaa tcaacagcaa tctgctggta gcgcgggcgg acgatttttt tctttccaga
     4501 cattttcttt cctctttctt tttttacttt ccctcatctt actatgctct agcaaaataa
     4561 gcaaaaaagg ggagaatagc ctcttgcaac gactgatcat cgcttgatct gttccttttt
     4621 tactgggcaa gctgtttgac agtcatcaca attcttaccg gattttaaac gagtatagac
     4681 aattgcgcct gcacttccaa agattaacac acttaataag aatgtcgcca tcctattcct
     4741 ctccttctaa tgacaatata gtataaaact ggttcttgga ttttggctga cggaacaaaa
     4801 cataaacgag tcctatcatc agtaaaatag caagatacgt ccctaacgct acctgtccac
     4861 cctccaataa gtgtcccaac tgataaatca caaaactaac taaataagct aaaccgcatt
     4921 gatagcctat ggcgatccaa gtccatttct tggcattcat ttcccgatgg atagccccaa
     4981 ttgccgcaaa acaaggcgca caaagtaaat tgaaggctaa caatgaataa gctgctgcgg
     5041 gagtatatgt gtgttgtagc acttgccaga tttctctgcc attttcggat acttccgcat
     5101 gaccaaacaa aataccaaat gtgccaataa tattttcttt agcgaccaat cctgtaatcg
     5161 ctgcgactgt tccttgccag tttccccaac caagcggctg aaaaacaact gccaggattc
     5221 gcccgaaaaa ggctaagata ctttggtctt cagaaacagg ttgagcataa aaattataag
     5281 aagagctgaa ccagatcacg atactagaaa caaagataat cgttcctgct tttttaacaa
     5341 aagacttgct gcggtcatag gtttgtctta ggaccgctga ccattgagga aaatgatatg
     5401 cgggcaattc catgataaaa ggagctgtat ctccagcaaa caaacgtgtt tttttcaagc
     5461 tgattccaga gaaaataatt gcagtcatac caagaaaata ggcagaagga gctacccaag
     5521 attgatgagg aaaaaaggcc cctgctacta aggcaataat cggcaacttt gcagaacaag
     5581 gcatgaaggt tgtgaccatg atcgtcaaac gacgatcttg ctcgttttca atcgtgcgac
     5641 tggccatgac gcctggcact ccacatcctg tagcaatcag cataggaatg aaggattttc
     5701 ctgaaagacc gaatttcctg aaaagtcgat ccatgacaaa agcgatcctc gccatatatc
     5761 cgcagtcttc cagaaagctc aagcataaaa acaatataat caattgcggt agaaatccta
     5821 acactgctcc cacgcctgcg atgatgccgt tcaagatcag ctcctgcatc catcccgcta
     5881 cttgccattc agctaacaat ctgccaacat gttctggtaa ccatgtacca aacaatgtgt
     5941 cattgatcca atctgtcccc attgttccta ctgtttgaat agccagataa tagatcagcc
     6001 acatcacaaa tgcgaagatc ggcaaggcta accaacgatt cgttgcaatc tgatcgattt
     6061 tgtcactcat cgataattgg aaagaatcat ttgacacaac acagagtgta catagttggg
     6121 taataaattc ataacgttca ttgaccagaa tcgtctcact atcgtcgtcc aatagtttct
     6181 ctgttaactg gatcagttgc tcgatctctt tttgctgaga aactgctatc tcgtaatcct
     6241 ctcttacctt ctgatctcgt tcaaaatatt tcattgcata ccagcgtttc ttgccgcttt
     6301 gttctcctag tacatcttca atttcatgta aagctgcttc taaacgtgga tcatatgtac
     6361 agggaacagg aatagaaggc cgcttttttg cagactcctg aatcttttcg attagttttt
     6421 cgattccttg ctttttcaat acgctgattg gtaaaactgg tacaccaaga ccataggaaa
     6481 gcttttctag attgattttt tttccttctt ttttgatgac atccatcata ttcagaccaa
     6541 tgatcatagg aatgtctaaa tccaataact gcgtagttaa ataaagattt cgttctaaat
     6601 tggtagcatc aataatgttg atgatcagtt caggctgttc ctgaaccaaa taatctcttg
     6661 caatcacttc ttctggtgta taaggagaca aagaatagat gccgggtagg tcctgaatat
     6721 gaatgttcgg attttttttc aacgatcctt cttttttttc tactgttacg cccggccaat
     6781 tccctacata ttgattcgaa ccagtcaaga gattgaacaa gctagttttc ccgctgtttg
     6841 gatttccgac taacgcgata ttcattcttt ttcctccctt tgtatcaaga ttagttcggc
     6901 ctctgacttt cgtaacgtca gttcataccc tcgcactgtc agttcgattg gatctcccag
     6961 aggggctagt tttcggataa ctatttttac atttcgcgtc aatcccatat ccattaaccg
     7021 tcgcttcact gcgccttgtc cgtggattgc tacgacgatc ccagtctctc ctgcttttaa
     7081 ttgatcaaga gaagtccagc tttcagagga agcagtcttt ttattgacaa agatttgacc
     7141 tagtatttcc cgacttactg ccactcgatg actttttacc agtaaaatgg cattgttgcc
     7201 tgataaattg acgacgacga ttttacttcc ggcaacgatc ccgatatttt gcaaatggcg
     7261 ttttgttttt tcttctgttt cgattcgttc gacttgataa acgaaattct gctttgcctg
     7321 cgctaaggtc tgcatattga ttcctctctt gtgaaataaa tcatgtatta aaatcattca
     7381 ttatatgttc taattttctt acgtgttacg caaataaatg agaatcattc tcacatagaa
     7441 tactctcggt aattgagaat gtcaattata cttgtgatat tatgagcgaa tctttagtat
     7501 tgatagataa aaatgagaaa aaaatatttc ttcatagttt tccacaattt tttctctatt
     7561 aaatttgcta ttttcccaca cgatacttgt tttcagctct tttttcaaaa acacaaaact
     7621 tagtattcca cagtttccac aacacaatat gtagtgtttg ttgtttgact tttttactaa
     7681 gagttttata ctatctatta tagataataa gaatgtgtta gaggaggcac tcaccatgaa
     7741 cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag atggtcaaac ggtttttaac
     7801 agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa cagcctgaag cagtagactg
     7861 gttgaaagca caaggattcc aaagtgttcc tgtcattact tctgatgcag aaacagttat
     7921 cggattccgt cctgatcagt tgcgtaaact tgccagctaa catgactaaa agaatcaaaa
     7981 cgtgatacat ggatttgaag ggcttgagac agcaccagtt tttgtcgcaa acttccaaat
     8041 ccttttttaa agcttaaaaa caatgcaatg atttgcaatc aagcatcatc aaatacgaaa
     8101 tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag atgtcagtta tttcaagctg
     8161 aataatgaaa tcaatcggcc agtcaatggt caaatccctt tgaataagga taaagaagct
     8221 ttagttgcct tttttgagga aaatgtcaaa ccaaatacga tgacttttct atctattatg
     8281 gaaaaaatca attacttgat cgatcaagaa tatatagaag cagaattcat tcaattgtat
     8341 tcaccatcat ttattgaaaa gctatatcaa tttttggacg agcaacattt ttctttcaaa
     8401 tcttttatgg ctgcttacaa attttattct caatatgcgt tgaaaaccaa cgacggttcc
     8461 gcttatctgg aaacttatga agatcgagta gccttcaatg cgctttactt tgcgaacggg
     8521 gatgaagaat tagcacttgc tcttgcagac gaaatgatcc atcaacgata tcagccagct
     8581 acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg aattggtgtc ttgtttcttg
     8641 actcaagtca ctgacgatat gaactctatc gggcgctcaa tcaactctgc tttgcaattg
     8701 tcccgtattg gtggcggcgt agggattact ttatcaaatc ttcgtgaagc cggcgctcct
     8761 atcaaaggat atgagggtgc tgcaagtggt gtcgttccag tcatgaaatt attcgaagat
     8821 agtttcagct attctaatca attaggtcag cgtcaaggag ctggcgtggt ttatttgaac
     8881 gttttccatc cagacattat tgctttcctt tcagcaaaaa aagaaaatgc agatgaaaag
     8941 attcgcgtaa aaactctttc tcttggcgtg atcgtacctg ataaatttta tgaattgaca
     9001 cgtaaaaacg aagatatgta tttatttagt ccatatagtg tcgaaaaaga atatggtgtc
     9061 ccattctctt atgtaaatat tacagctgag tatgatcatc tagttgcaaa tccaaacatt
     9121 cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa tctcaaaatt acaacaagaa
     9181 tctggctatc cttacgtaat caatattgat acagcgaacc gcgagaatcc aatcgatggc
     9241 aagatcatca tgagtaattt atgttcagag attttacaag ttcaaacgcc ttctgtgatt
     9301 aacggcaaac aggaatatga agtattagga acggatatct catgtaactt aggctcgaca
     9361 aatatcgtga atttgatgca aagccctaac tttggtcaat ctgtgcgtac gatgactcgt
     9421 gcattgacat ttattacaga tgcatctgat atcgatgtcg taccaccgat ccaaaacgga
     9481 aataagctga atcatacgat tggattaggt gccatgggct tacacactta tcttgcaaaa
     9541 gaacaaatgg aatatggctc agaggattca ttagacttta caaatattta cttcatgctg
     9601 ttgaattatt ggacacttgt tgaaagcaac aatatcgctc gtgaaagaaa acaagttttc
     9661 cataattttg aaaaatcagc ttatgctgac ggcacttatt tcgaaaaata cgtaactgga
     9721 gaattccagc caaaatcaga aaaagtcaaa gcattgtttg aaggcatttt tatcccaact
     9781 gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag atggactgta tcatcaaaat
     9841 cgcttggcag tagcaccaaa tggctcaatt tcatatatca acgatacgag tgcaagtatt
     9901 catcctatca ctcgcatgat cgaagaacgc caagagaaga aaatcggtaa aatttattac
     9961 ccagcgcctt atctatccaa tgagacgatt ccgtattaca cttctgctta tgatatggac
    10021 atgcgtaaag taatcgatgt ttatgcaatg gcacaacaac acgtcgatca aggcatgagt
    10081 ttgactttat tcatgcgttc tgaaatccca gaaggcattt atgaatggaa gacaacaacg
    10141 aagcaaacga cacgtgattt gaatatttta agacattacg ctttccacaa aggaatcaaa
    10201 tccatttatt acgtacgtac ctttactgat gatgcagaag aaatcggaag caatcaatgt
    10261 gaaagctgcg tgatctaata tcatagatag attcgcttat tcatatttta attaaaggag
    10321 cattctcatg tcagaaacat attatgaagc aataaactgg aatgaaattg aagatatcat
    10381 tgataaatcc acttgggaga agctgacaga gcaattttgg ctagatacac gtatcccact
    10441 ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg gaaaaagata cagtaggaca
    10501 tgtatttggc ggtttgacac tgctagatac tgtccaatct gaaagtggca tggatcagtt
    10561 gcgtaaagat gtccgcactc cccatgaaga agccgttttg aacaatattc aattcatgga
    10621 atccgttcat gccaaaagtt attcttctat ttttagtaca ctgaatacga aaaaagaaat
    10681 cgaagaaatt tttgactgga caaatacgaa tccttatctg caaaaaaaag ctgagcgtat
    10741 caatgaaatc tacaaacacg gtacaccgct agaaaagaaa attgccagcg tctttttaga
    10801 aacattcttg ttctattcag gtttttatac tcctctttat tatttaggaa acaacaaatt
    10861 ggctaacgta gcagagatca tcaaactaat catccgagat gaatctgtac atggaaccta
    10921 catcggttac aaattccaat taggattcaa cgagttacct gaagcagaac aagaaacgtt
    10981 aaaagactgg atgtacaatc ttctttatga gctttacgaa aacgaagaac gttatacaga
    11041 agaattatac gatccaatcg gctggacaga agaagtcaag acattcctac gctataatgc
    11101 caacaaagca ttgatgaacc tagggatgga tccactcttt ccagatactg ccaatgatgt
    11161 caacccaatc gtaatgaacg ggatttcaac aggcacaagc aaccatgact tcttctcaca
    11221 agtcggaaac ggctatttgt taggtcatgt agaagcgatg aaagacgacg attatttgat
    11281 tggattggaa taaggattta ttttaattca aaaatttttc gaaaaataaa aagagtttag
    11341 cgtacagata ttttaaatgt acactgaact cttttttatg cttattcact tgtttcttcc
    11401 agcacttttg gactgtctaa cttttccgaa acatgttcca ttcttctcaa taaatagatt
    11461 ttccggatat ttaatattat cgtcttaatc actgcataga caggtatgca aatcagcatg
    11521 cctaatattc ctgcgacact tcctgctcca atcaatacca agatgatcgt caaaggatgg
    11581 atattcattg atttcccaaa taacaacggc ttgatcaaat ttccatctaa ttgttggatc
    11641 actaaaatcg acaacgccat atacaaagct tgaaatggag agataaacaa accgacaatg
    11701 attgctggta ccgctccaat aaaagggccg acatacggga taatattcgt gattccgcaa
    11761 atgaccgcaa gcaacaagct ataaggctga tcgaatattc tcattgccaa aaagctcatt
    11821 acaccaataa tcaaagcatc taagatcgta ctgctgatat aggcagataa tgtttcgttt
    11881 aattctttta ccgtttggcg taactcttta cgaatcgctt tcggaaagaa gtgagacaac
    11941 gcatcaataa atttgtgccc atccttgaac ataaatagca aaataaatgg aacggtaaat
    12001 aacaaaataa aaaattgcat cataaatgaa atgatttttg agacacttgc agataaactg
    12061 actaatatga tattgaacaa attactaaga gataaatttg ctgatgctaa ttgttcttcc
    12121 aaattgaaat ttttaaactc ttcccgctgc gccaattcat ttaaccattt gcttgtttca
    12181 tctgcataca ttggcaagct ctgggtcaat tgaattgtct gttccattaa ctgaggcact
    12241 acattcatga cagttaaaac aactaaaatg attacgacca aaaacgtcaa taaaaaaccg
    12301 aacactctcg ggatccgatg cttttcgaga aaaacaacga ttggatcgaa catataatag
    12361 ataaaaccag caacaagcaa tggtaaaaaa actgccgaaa gcattttcaa tattggacta
    12421 aaaatattcg gcatttgtaa aataaagtac acgccgatga tcgtgagtac tagctccact
    12481 gtccagaaaa ataattttga ttgtttaaaa cgtgattgca tacatctcct ccaaggtcct
    12541 ttgatacttc tattctatca tttaacaaag agttacgcgc tgagattttc atttgcttaa
    12601 aaaaaacaaa aaaaataaac atattttaat gagtagacga gtacttaaac aaaaacacgg
    12661 tttggagtag tgacaaaaat tatactgcag tccctttttc caaataaacg gtgttcaaaa
    12721 gcttgcatct tcgtaataag cccagccaaa cgaaaaatat aaggaactat ttttatttgg
    12781 ctgggcttat tacttgatgt aggacatcta ttttacaacc tcagatcatt tattgcgcaa
    12841 tactgactaa atgcaacttt gtgatatttt tgtgtacgga caactcctcg atcacatcgg
    12901 cataagtgag cccttttgga agatcaatcg tatagatatt tttgtacact cggtagtcat
    12961 caacgaattt tacgtcaaaa ttcacatctt cgattacgat atttttctct tcaaaataat
    13021 cggtaagaaa atctttggtt tcttttctat ggataaattg gatttctaac tttttagtcg
    13081 taggtacatg gatcacgcgt ttcactactg tgagtgcaat agcaatcccg ataaaactga
    13141 tgatcgcaat ggaatagtaa cccatgccga tggcgatccc taatccagca attgcccata
    13201 aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt gacgatgatc gttcccgcgc
    13261 ctaagaaacc gacaccactc accacttgag caatcagcct tgcttcatct gaacgaatcg
    13321 ttccagcaag ctgtggatac tctaaagcat ctcttaatgc accactagcg atctcaactt
    13381 ggatcaatgc aatgatacat gctcccatac aaactaaaat atgcgtacgc atacctgctg
    13441 gacgattttt atattgtcgt tcaaaaccga ttgctccgcc aaatagcata gcgatcccta
    13501 aacgtaaaat aatctctgga attgtcaata ccatatcatt catctaaaca ccctccagtc
    13561 caatatatca tgtctgaaag gagcagacaa aaaaagtgca atttttacct cttacgaaaa
    13621 aaggactgtg acaaaatttt tgtcatagtc ctttttatga ataaacggtg ttcaatcagc
    13681 tgaccctcgg tattactcaa caaacggcaa aatatgggga gctatttttc ctttgtcttc
    13741 tctaatactc aggtcaaaac gctgatttca caacctcttt agcctactat tgaaaattac
    13801 aacttacttg ctgcttcttc atcgatgatc acaacaacat tgtcgtgatt ttgcaataca
    13861 cttgctggca aatgattcgt tacaggtcct tcaatcatcc ctttgattgc atcagctttg
    13921 ctttcgccaa atgccatcaa gacgatttct ttacttttca taattgaacc gattcccatt
    13981 gaaacagctt ttgttggtac atcttctgct ttatcgaaga atcgtttgtt tgcattgatt
    14041 gttgattctg ttaagtcaac tactgaagtc gttccgtcaa atgaagcacc aggttcattg
    14101 aaaccgatat gtccattttg cccgattcct aagatttgga tatcgattgg atgtgcatcg
    14161 atgatttgat catacttttt gcatgctgct tccagatctt ctgcttttcc atctggtaca
    14221 aacgtttctt tgaatggttt tttatcaaat aattggacat ccatgaaatg acggtagctt
    14281 tgttcatctg aaccgcctag tccaacgtac tcatctaagt ttacagaagt catttcactg
    14341 aaatccaaat cgcttgaagt catttcttta tataaagtaa ctggtgtgct tcctgttgcc
    14401 aatcctaata cttttgcacc tttgttcata ccttctttga tgatttcaaa tgcttttttt
    14461 ccgccttctt cagcgttttt tacacgaatg atttccatag tgattgctcc tttatatttt
    14521 ggtatagccc aattatagca ttcttttttt ctttttacaa taattagtat agaccaaatt
    14581 gcattttttt cattttcttg acaataaata ggaatccgtg ttaggtttca taagatggtt
    14641 caatcaaaca atccatgttg catgaaaacc aagcaagtga ttcactgtgg acgctttatg
    14701 tctgcagttt ttttatgaaa aaggagttat tatgagtata ctgacattaa acaacattac
    14761 ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat ctgcaattaa atgctggcga
    14821 acatcttggg ctgattggac aaaacggagc cggtaaaagt acattgatta aaatcatcac
    14881 tggtgagatc ttaccagatg acggacagat ccattggcaa aaaaatattc acaaaggtta
    14941 cctcgatcaa tatgtggaag tcgatgaaac attaacaatc gaagaatttc taaaaactgc
    15001 gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt ctttaccaaa catatagcga
    15061 gaccttcgat gattccttgc tggaaaaagc tgggaaactc caaacggaat tagaccaagg
    15121 agtgttttat caaatcgaca ccttagttgc tgagatgagc agcgggttgg ggatcgatgt
    15181 cttaggttta gattcactgt tgaaaaatct aagtggtgga caacgttcaa aggtgatttt
    15241 agcaaaacta cttttagaaa acccagatgt tctgatctta gatgaaccaa ccaatcactt
    15301 agatgatcag catatccaat ggctgacgca gtttcttcag gattttgacg gtgcgtatat
    15361 cgtgatttcc catgaccaag aatttcttga tcagatcacc acccatatcg cagacatcga
    15421 gtttggaaag attactaagt atactggcca tctaaaacaa gcattgaaac aaaaggaaca
    15481 aaatcgcgaa agctatttgc gccaatatca tgctcaacaa aaacaaatcg aaaagaccga
    15541 agcttatatt cgaaaataca aagccggttc ccgatcaacg atggcaaaaa gccgacaaaa
    15601 acaattagat aaaatcgaac ggctgacgcc tccagcttca gcaagtaaag ctgtctttga
    15661 ttttccattc tcgcctattg tcacgacatt agcagtggaa acaactgatt tagtcatcgg
    15721 atatgatcga ccgctacttg cacccatcaa tttgaccatc cgctttggtg aaaatattgc
    15781 cattcgaggc ttcaacggga ttggaaaatc tactttatta aaaacactga tcggtgagat
    15841 tgaaaaaata agtggcgatt tccacttccc agataacaca aaaatcaatt acttttcaca
    15901 agatctgtac tgggaaaatc cattagaaac accactacaa tatttaagca gtcaatttcc
    15961 aaaagcgact atcaaagaat tacgtcgcca actagctaaa gcagggttag ttaatcaatt
    16021 agcaagtgaa cctctcacta cattaagtgg tggggaacaa actaaggtga aacttgcaca
    16081 gttgacaatg aaccaaggta atttactgat tctcgatgaa ccgacgaacc atatcgatca
    16141 agaaacgaaa gaaagcttac aagacgggat acagaaattt ccaggaacag taatcatcgt
    16201 ctctcatgaa caagaatttt atcaagatct tgtgcaacga gtcattgaga ttgaaccaaa
    16261 ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga caaaagtttt gtcacaactc
    16321 cttcttctga ataaatggtg gccaattagc tgaccttcgg tattagctca aaaaacggca
    16381 aaatatgagg agctattttt atttggctat tcttattact tgatgcagaa cagcttttca
    16441 caacctcttt tcgtctattt tttattttcg agatttctct ttcctttaaa aacaactgtt
    16501 tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa ccgttacacc taaccagcca
    16561 tactgctgcc acattgtcgt ccctagcaat gaaccagccg cacctccaat aaagtacata
    16621 aacataaata cagtattatt tcggttgctt gctgcttcac ctagcccctg tactcgtgtt
    16681 tgattggcga cttgaccaaa ttgattcccg acgtctaata aaataatagc taaaagcaaa
    16741 atggccaaat ggcttccgcc aaccaataaa gcaataaagc ttattgtttg cataacaagt
    16801 cccatataca cgatttttct ttcagaataa gaatctgcca gtcgtcccac gataggtgca
    16861 gcaactgctc cagacaaacc aaaaatcgct aatatccctg cttcgaatgt tccccaattg
    16921 taggcgggac tgctgatata aaaaatcaac gtagaccaaa agatcgaaaa tgttccaaac
    16981 atgaaaaatc cactgactgc cgcttctctt aatagacgtt gagaagcaac taaatgcggc
    17041 aaacttttaa gtgaatcaag gtatgaaaca gctgtactac tttgctggac agaacgcggc
    17101 attttccagt aaagcagaag tgtaagaatt ccgactgcac ataaagcaaa taaatagatc
    17161 atccgccaag aaaaaagact ggcaacaata ccagataccg tccgagaaag caaaatcccc
    17221 acgagtaaac cgctaagcaa tgtacccatg accttccctc ttgcttttgg tcccgctagt
    17281 accgcaccgt aaggaatgat gatttgcggc acgatcgaaa gtaatcctat taaaaaagaa
    17341 gcgcatgcaa ataacgtaaa cgaaggcgca aaaaaagcgg ccaacagaga gacggcagat
    17401 aacgctgcca ttcgaatgat caattttgga cgatccacca cgtcccctaa aggaactaaa
    17461 aacagtaacc ctaacgcata gcctaattgg gtcaacatcg tcaatatccc gactgcactt
    17521 gtgctaacag ataaactagc tgctactttt gttccaatcg gctggatgta atacatattg
    17581 gcaacaacaa cgccacaagt gattgataat agtaaaataa gcgaagttgt taaaggtttt
    17641 ttttcattac tcttcattga tttcccccat ttcctatctt agtttacaac ttatggaaaa
    17701 gattggaaat agaaaaatac aactattcgc cacacttatt atttgtgctt ttgttttcct
    17761 tgttttttct tgcgatcttt ataaatgaag acaactaacc ctactagcac agtaacactt
    17821 gcaaccaacg tgactgcagt tttgttatat ccgtgaaaca gagcttctcc accacacgca
    17881 tacaacacag acgaagggat gactccaatc acgatcgaca atatgatttg ctgtagaggc
    17941 aatttcatcg catctgcagc aaaattgacc atagaagatg gaataaccgg tatcatatag
    18001 cctaacatta cacctatttt tggatgtttc atttgtcgga ttgcttgcac ccaatgattc
    18061 gtttcggttt tcttatctaa aaattttaag cgatgcataa ggaaaattgc agtgatgtta
    18121 ccaagagcat tacccaaaat gttgatgatc gcacctgtca gtggtccaaa gctcaaacca
    18181 gcaatgactc caactactga agtaggaata cctggaacag cacacatcac cgctgtcaat
    18241 acgatcaaga gtatcgatgc aaaagtccca tgcgaacgaa tatgtgcgag tagctctgcc
    18301 ttactcgctc tcggatttaa aaataattgg atgtctgtct gatattctaa atacaaccga
    18361 tagcccacta ccagtatcag aatgattccc aacgagatca atatcaatcg gattattttc
    18421 tttttcatat ttcctcctga aaaaaatcta tattcattaa ataatagttt aacataaaaa
    18481 caaaaaagca ccgaaatcca acataggatc tcagtgcttt taaatagtaa aaattattta
    18541 acagttacag atgcgccaac ttcttcaagt ttagctttca attcttcagc ttcttcttta
    18601 gatacgcctt ctttaactgg tgcaggagcg ccatcaacta cagctttagc ttctttcaag
    18661 cctaaaccag ttgcttcacg aactgctttg ataactttaa ctttttggtc tccagctgca
    18721 gttaattcta cagtgaattc tgtttgttct tcagcagcag cagcgccacc agcagcagct
    18781 acagctacag gagcagcagc agatacgcca aattcttctt caatagcttt aactaggtcg
    18841 ttaagttcta ggatagttgc ttcttttaac tcagcaacga tgttttcaat gttcaatgcc
    18901 attataattt cctccgttta attgttcttt tttatttaat gctttatgcg gcaattaagc
    18961 tgcatctcca tcttctttgt ctgccactgc tttgacagcg taagccacgt tgcggactgg
    19021 cgcttgtagt acagatagca acatagatag aagtccttcg cgatttggca attttgccaa
    19081 tgctgtgatt tcttccaatg aagagacttt accttcgatg ataccgcctt tgatttctaa
    19141 tgcttttgct tctttagcaa attcgtccat gattttcgct ggtgcgacta catcttcgtt
    19201 actgaatgca acagctgtag gtccagtgaa tacttcgtcc atgccttcaa ggccagcttt
    19261 tttagctgca cgtgaaagaa tagaattttt gataactttc atttctacgt tagcatcacg
    19321 aagttgttta cgtaaacgtg ttacttcatc aactgttaat ccgcggtagt caacgactac
    19381 tactgatgca gctgctgtaa atttttcgct tacttcatca acgattgctg ctttttttgc
    19441 gataattgct tcactcattt ttgagtttca cctcctgaat tttgatggaa gagttttttg
    19501 cactgaaaag gcaagtcact tttatagtta ctttgctcaa ccagtattat tcatcaccta
    19561 ttcacttcgt tcatagtgat tctactcgct ttgctcgaaa ggaaactcct ttcgctttgc
    19621 tcaaccagta ttgttcatca tctcttcact tcgttcatag tgattcacaa taaaaaactc
    19681 tatgccaccg cagacataga gggactattg aatatattca ataaacgtcc tcggtaggaa
    19741 attaaggctt gatgccacct actgtcttcg gtacaagtta attattaacc tcaactagct
    19801 tagcatgcta aactagttga gtcaagaatt atttttcagt tggatcaatc aacattctaa
    19861 tatttaatta gaaagaagct tggtcaacat ggataccagg tccaaatgtt gttgtgatcg
    19921 ttacattttt catgtattga cctttagctg ctgatggttt agcttttagc aatacatcat
    19981 ggattgctgc aaagttttca actaattttt catcgtcaaa tgaaactttt ccgattggta
    20041 catggatatt tccagctttg tccacacggt aagttacttt accagctttc acttcgttta
    20101 ctgcttttgt tacgtccatt gtaactgtac cagttttagg gtttggcatt aagccttttg
    20161 gtcctaatac tcgtcctaga cgacctacag tagccatcat atcaggtgtt gcaacaacta
    20221 catcaaagtc aaaccatccg ttttggattt tttgaaccat gtcgtcgtca cctacgaaat
    20281 ctgcaccagc tgcttcagct tcttttgctt tgtctccttt agcaaaaact aaaacagttt
    20341 gtgttttacc agttccgttt ggtaatacaa cagcaccacg gatttgttgg tctgcttttt
    20401 tagggtctac gttcaaacgg tatgcaactt caactgttgc atcaaatttt gcaatgtttg
    20461 tttcttttgc taaagctact gcttcggcaa caggataaac tttacttgaa tcaacttttt
    20521 ttaatgcatc ttgcattttt ttgctctttt tagccatttt ggttcctcct tgattgtggt
    20581 tataacggtt gtacctccca cgtgttctat atctttcaat atagagccaa ctgagaagct
    20641 gctgtcttag ggtgtataga ttattctaca gtgatcccca tgcttcgtgc agttccttca
    20701 accatgcgca ttgctgcttc aacgttagct gcgtttaggt cttccatttt taattcagcg
    20761 atttctctta cttgatcgct agaaactttc gcaactttat ttttgtttgg ttcgcctgaa
    20821 cctttttcga ttttagctgc ttttttcaat agaacagcag ctggcggtgt ttttgtaacg
    20881 aatgtgaatg aacggtcttc gtatacagaa ataacaaccg gaatgatcaa acctgcttga
    20941 tcagctgtac gagcgttgaa ttcttttgtg aatcccatga tattgatacc cgcttgacct
    21001 agtgcaggac ctactggtgg agctggagtt gctttacctg caggaatttg caatttaacg
    21061 attttttcta ctttctttgc cacgagacat acctccttaa gtccgtgatg tggttaattg
    21121 gagatgcata tttctcctcc cacgttttat ctgatctgaa aaaatccaga caaataatgt
    21181 gcaaaacaat gcacactggt acagtatagc aaatttattt gttttttcaa gatttttttc
    21241 tgctattttt acattttgct tgaataaacg ctaaatttat tctaaaaaaa caacaatttt
    21301 tcgttctcat ttcttctttt atgttacact tgcgctagca tggttcaaca gatgaaagca
    21361 aacaataaca cggtttttta gacccttgta caaagattcc tacataacaa aatgaggtgg
    21421 agaaaatgaa agatttaagg tatagaagtg tctttgatat tattggacct gtgatgattg
    21481 gtccaagtag ctctcacact gccggagctg ctagaatcgg gaaaatcata cgtagtatat
    21541 ttggcgaaca gcctgattct gtagatattt atctatatga atcatttgca aaaacctatc
    21601 gggggcacgg aacagatatt gcattagttg gcggcctgtt agggatggaa cccgatgatg
    21661 aacgtttagc tgattctttg aaattggcat atgaagccgg tatggaagtc ttgtttgttc
    21721 ctaagagtga aaaagccgaa catccaaact ctgtaaagat gcttctaaaa aaaggaacac
    21781 gaaagctttc agtcactggc atttcgatcg gcggtggcaa tatccagatt tctgaattga
    21841 atggattcaa aatttcttta agcatgggta ctcctacctt gatcgttgtc caccaagatg
    21901 ttccaggtat gattgctaaa gtgactagta tactttctga gaccaatacg aatatcggca
    21961 ctatgacagt gacacgggaa tcaaaaggtg aaaatgccat tatgataatt gaagtagatg
    22021 atcctcaagt agaagaaacg gtaaaaagac tcaaacagct gccaaatatc gacagcgtaa
    22081 attatttcga ttaaggagct aaaaaaatgt ttttatcgat tgaagaatta gtagatcaag
    22141 caaaagctta tccaagtgta gcagaattga tgattgcagt tgaaatggaa atgagtggcc
    22201 gttcccgtag tcacatcatc gagatcatgg aaaaaaacct ctcagtaatg gaacaatcaa
    22261 tcaacgaggg aaaagaaggt gttacctccg tcacaggaat taccggtggt gatgcaaaaa
    22321 aattagatga ttatttaaat gaggcagact ttttaagtgg agaaacaatc ttaaaagctg
    22381 tacgcaatgc tgtagctgtt aatgaagtca atgcaaaaat ggggttgatt tgtgctacac
    22441 cgactgccgg aagcgcgggt gttgttcctg gtgtgctgat ggctgtccga gatcggcttg
    22501 atttgacaca tgaacagcaa ttggaattct tatttaccgc tggcgctttc gggttagtca
    22561 tcgcaaacaa cgcttctatc agtggtgcag aagggggatg tcaagcagaa gttggttctg
    22621 caagtgcaat ggcagcagca gctttggttt gtgcaaaaaa tgggactcct catcaagcag
    22681 cacaagcagt agccattaca ttgaaaaata tgatgggact gatttgcgat cccgttgcag
    22741 gcttagttga agtcccgtgt gtcaaacgta atgctttagg ctcttctcaa gcttttatct
    22801 ctgctgatat ggcattagcg ggtatcgaaa gtgtcattcc gccggatgaa gtcgttgctg
    22861 caatgtacca agtcggacgt caaatgccgt cgattttcaa agaaacagca gaaggtggct
    22921 tagctgttac gccaactgct aaacgacttg cggaagagat tttaaataag agggcgtgat
    22981 aaagctcctt aatttcgagt attaagagat tagttatctt cataacaaaa aacgaagaga
    23041 ggactaattg cccttctctt cgttttttat taatccatca atttcgtata ttttacttca
    23101 tcgatcgttt ttgttcctgc aagttgcatc gtgatcgaca attctttgtt caggtgttca
    23161 aaaacagatg ttactccttc tgccccacct agattcaacc cataaatcac tggtcggcca
    23221 atcgcaacta agtcagcacc acttgccaga gctttgaaga catgctctcc tcgacggaca
    23281 ccagagtcga aaatcactgg tacacgtttg ttgactactt cggcaatttt tggcaatact
    23341 tcaaaggacg caggacctcc atctagttga cgtccaccat gattggatac ccagatacca
    23401 tctgcccctg cagagatagc gattactgca tcttctggcg attgtattcc ttttacgata
    23461 actggaagat gggtgatttc tttgattgtt ttgatgtcat ctggtgtcaa gccttgtttt
    23521 gcagctgcat aaatttctgc gattcctttt ccttcgccat taccacttgc actttgttcg
    23581 ctatacgcag ctaagttagg cattggtaat ggaaattgga attgattgat gacgtcttct
    23641 tcacggtaac caccgagcgt tgaatctgcc gttaaaatga tagctttagc ccctgcttct
    23701 actgctttgt tcaaaatgaa ctcattgaat ctgtcgtctt tactcatata taattggaaa
    23761 aactgcggtg cgcctggcgc tgcatcggct gcgtctttga ttgtcgtatt tgcataagtg
    23821 ctgatggaaa agattgatcc agcagcagct actcctttag ctgtatcagc ttcccctttt
    23881 tcatgtgcca atccttgggc cgctgatggc gcttggataa tcggtgtatc taattcaatt
    23941 ccgaaaacag atgtatgcaa atcggctgag tcgattccgc gtaatatccg tggcatgatt
    24001 ttctttgtat taaaagaagt cgtattttct ttcatcgtcc actcatcttc agaaccaccg
    24061 cgaatatagc caaaagctcc tgcttccatg cgctctttta cacgaccttc tagttaggca
    24121 atgtttacaa tatcgattgg atgttcttca ttacttgtat gataagtcat agtagatcct
    24181 tctttcatct tcttgattac atataaatca tactaaactt acaaaaagta agcaaacatt
    24241 tactctcaga tacttttatc gctattcttt ccaaataaat cgacttacaa gctgatctac
    24301 ttcttggttt tcatgaagca tactatgaga ggcattttca cctgtcacta ctttttcctc
    24361 tatttcattt ccgcgttgct tcaacaacgc atagaccgct aatgcactgt ttaacggtac
    24421 tgttccatca ctcaagtctg tttcatctag ttgaccagca atcaagaaaa atcgagtggt
    24481 tataggaata ttaccgatca tttgctggta atcttgatag cggttagaaa cgacagcagg
    24541 cccctttgct aactcatctg tcagagactg cgcactatca tcgacaaaat cattaaaagg
    24601 cgcgccaatt gctgttaatg tctgaacttg tggcaatgac gtatcctgtc catacgttcc
    24661 taaatagcgt agtgcactaa ctcctcccat ggaatgcccc actatgttga ctttatctat
    24721 gccgtagttt tctttcagat acaaaagaca agttttgatc cattctgctt gattccactc
    24781 gttattttta ttatcttcaa acaatacttg gaccatcgga ttgtctgctt ttccagacaa
    24841 atctcctgtt gcttgtattt gtccatctgt tcctactgtt aaaaccaatt cttttactgc
    24901 ttgctgattt ttttccaaac gccgcaacat ccctttaaag gaattttctg tcccgctgta
    24961 gccgtggaaa aaaagtgttg gaatagtggt tttttctatc actttactgg attctgccgt
    25021 tttttcttta tcagaaactt cagtagtatt tatcttcgtc gtctggcaac cacttaacgc
    25081 aaacaaactg ataaggacta aagctgctgc agttgcacgt ttgcataaat ttttgattgg
    25141 ttctgtcata cacctcttcc tttccatata gaaaaaagaa agacttgaaa ggccaatcat
    25201 atgatcgaca tttcaagtct ctttattaat cgattttatc tacttgatcg aaatcaagtt
    25261 ctgtactggt ttcacgtcca aacatatcga tgttgacttt caatttttct ttttctccgt
    25321 caacttctgt tacttgtcct tctaatccag caaatgcgcc ttcgatgatt ttcacgactt
    25381 cgccaacttc tacttctaga tcattttgtc gcaggctcat accgatagaa cgcaagatat
    25441 gattgacttc ttctggaagt aatggtgctg gtttgcttcc ggctccgtgt gaaccaacga
    25501 atcctgtcac accaggtgtg ttgcggacaa cataccatga agcgtctgtc atgatcattt
    25561 caacaagtac gtatcctggg aatgttttat gaacgatttc tttttctttc ccatttttga
    25621 cttcagtttc tttttcttca ggaacaacga cacggaaaat aaagtcggcc attttcatac
    25681 tttgcgcacg agattcgata tttgctttta ctttgttttc atatcctgaa tatgtatgca
    25741 agacatacca gtttttttca aaagattcca ttgaaggctc ctcatttcat tgtaaaataa
    25801 aaaaacctca gtacacccga agtttttctt tcgcttcatt atagcactaa aagtacgttg
    25861 tggctagcga gttattttaa aatccagcca aataagcttt ggatggctgt atccattacg
    25921 taaaacatca atccgaaaat aatagaagtt tcgatgacta ctaacgtatc tttacggagt
    25981 tgttttctgc ttggccaagt tacttgtttg atttcatctt ttacgctttt tagaaatttc
    26041 atgagacaac ctcccttgct actgtttatt tcgtttcttt atgtaatgta tattgattac
    26101 aatatttaca aaacttattg atttccaaac gttcgccgct ttttccttca ctgactgatt
    26161 ttgtgtagtt gcgagaccca caaacggaac aagcgagtgc tgcttttttc tttgccattt
    26221 acttctgccc ccttgctgag aatcagactt atacttcata acagtatcac gtaacttttt
    26281 acttgtcaat ttgtgaaata aactatctat tgttttggat aaaattatga tatgattaat
    26341 ttgaaattaa gctaaaatga gacaattgga ggtatagcga tgctattaaa aacacttgta
    26401 tataagaaaa aagatctgac aaccgttaca gagactagta cattagaaga agcattaaaa
    26461 attttagaaa cttccggata ccgttgtgtg ccgatcttag attcttcagg taatattttc
    26521 cgtggaaaca tttacaaaat gcatatctat cgtcataaag caaatggcgg tgatatgacg
    26581 cttcctgtca ctcatctttt gaaaaacgct acaaagttca tttacctgga tacttctttc
    26641 ttcaaagtat tctttacgat caaagagctt ccttatattg ctgtccttga cagtgaaaat
    26701 catttttatg gtattttgac acatagtacg ttgcttaata tgctctctca atcttggagc
    26761 gtagaacaag gaagctatgt actgacaatt gcttccacag gaaagcaagg agatttggct
    26821 gctattgcaa aaatcatttc aaaacacagc agcattgcca gctgtattac tcttgatatc
    26881 gaaaaagacg aatacattcg acgtacattg atcacgctac cggctaacac tgaagcgaaa
    26941 gtatgtgaaa cgatcgtcaa ccacttagag aaaaagaatt ataaagtggt agaaattgaa
    27001 aatttgcaaa accaagaagc ataatcaaaa agcgaaaaaa gaaaacagct aatctgcttt
    27061 cttttttcgc tttttataat tagacacgct tttcaaaatc aagcgcgcga tctaaataat
    27121 acatcaatgg tgctgaaaca gccatgacga taaactcact taatgcaagc gtgccatagg
    27181 ttgtccaaaa tgcttggcca cctgttggaa ctaacatata agcaatcagt cccatgctga
    27241 tggtaaagaa gatcgtgttc aatactaaac gcatctttac atttttcact ttgttttgca
    27301 ataaagctgt caggcctaaa gaaagcaatg tttggccgcc accaaaaacc acatccatga
    27361 taccaaaacc aaagaaggca ttatagacga tgaccccgcc taaaactccc caaagcaatt
    27421 tacggttaaa gacaactaaa tgattcaaac tttctgaaat acgaaactgg atcggccctg
    27481 aggatacagg cgcaactaaa atagttaacg ctagataaag agccatgatg atgccattta
    27541 caacgattac ggataaccgt tgtctgcttt tcgttttttg cattttctct cctcttttct
    27601 cctagttttt ttaacgcggg atggttgatg aaccgcggtt atgtgaaaca aacaataatt
    27661 ataacagact tttcatttat ttcaatccgt cttatctgct tttaaaaaag caaaagctta
    27721 aacaatgtaa acgtttgcta aaaatcgtgg gatatggtat aaagtaagtg taaggggtca
    27781 taagaattcc cagtaaagca gacgaagtag gatctgcaag aacagaattg ttgtgaaact
    27841 taaataaata aaaaaggatg tgggaatttg agcaacctct tactagagaa cggtctagca
    27901 agcagctgaa gatctaagtg gacatgccga accttggaac ttctccttac gtaagtaatg
    27961 gatcatgcgc ttgctaggct tttttgtgtt tttcggtctt cggacctttt tcattttcct
    28021 agacttctgc tataataacc aagaattaat tttgtaacag atttttttga aaaaacaagt
    28081 ttctttcatt caaaaaatag aaaaagcttg ctaattattg gttaaccagc aaacaggagt
    28141 attaaaatat cttgttttat tgaatagcag gaggaaatca tgaaattcca ttatcatata
    28201 ttgatcaatc catcggcagg cagcggaaat ggctataaag tagcagaaag aattttaccg
    28261 gtattaaaaa ataagcatat tgactataca cttcattata gtgaatacaa aggacacgat
    28321 gctgagattg ctgaaacttt ggcaaaagaa acattgattc cttggataga agaagaacag
    28381 aaaacaatcg atactttccc ccttttgatc atcgtaggag gtgacggcac cttacatcag
    28441 gtacttgata ctttctatca gatggaagta gaattcccag ttgcttatat tccagcaggt
    28501 tctggagatg attttgctcg aggggcaggc cttccaaaaa atcccaaaaa aggacttcag
    28561 ctgattttag cagctcaatc accagaaaaa gtccacataa tggcttatga agaaaaaata
    28621 agtgaaaaaa aaggaatagc tgttaataat ttcggtatag gattagatgc agctatcgtc
    28681 catgcgacaa atcattcttc cacaaaaaag cgattgaaca aatacaactt aggatcattt
    28741 tcgtatttat tctctattct ccgtgcttta ttcactcaaa aaggatttcc aattctggta
    28801 gatgcaggag gaaaacaatt aagtttttca aacgcatttt tatgtacagc aacgaagcat
    28861 cctttttttg gcggaggaat cgcgattgtt ccaactgctg atgcgtctaa accagtcatt
    28921 gattttgtcg tggtcgaacg aatcaatttc tttaaaatct tgtggctcat tcttttactt
    28981 atacagaaaa aacaattgaa atcgaaatat ttccatcact acacaacaaa cagattacgt
    29041 atcgtttcaa caacaccgca acatggtcaa gaagatggag aagaaatgga aaaacgacct
    29101 tttgatatca caataaccac aactaaacag ttgttttggt gttaataaaa gaggttttgt
    29161 cagaagctac atctggaaca ccgtttattc ggaaaaaagg actgtgacac gacttttgtc
    29221 acagtccttt ttttactatg cagttaattc tttgcccaat tgggttgcca tcgatagttt
    29281 ctttctttac ccaaaataaa ttggatcatt gaaaacaccg cagcaaaagc aactaagaca
    29341 caaccattga accaaaaaga ttctgcaaat gtttgattgt aactgagtgt ttcactgatc
    29401 aagttattat tttgaacaaa ccaatatgtt ggtgtgaatg ccgctatttt gctaaccatt
    29461 tctggcaagt attctgctgg tacaaacacc ccaccgataa agcaactgcc tgtgataaaa
    29521 atatctttga tcccaccgct ggtttctgag ttttttacca atgaagtgat acaagccgaa
    29581 aagctaatga tcggcaataa aaacaagaaa gtatttaaaa tatgatacca taccgttata
    29641 tcccacgagg tgtggatcac taacagaacc attagaagta agaagccact gaacaacagc
    29701 agcgagtaac taatcaaact aaaggttact ttgcgattca agtttcgttt ggtaatgggc
    29761 gcactctggt ttcgtttact gatctctttg cgattaaatg ctagattgat gacgctgaaa
    29821 ccgctaaaaa tggatagaaa caacccataa gacaagaaat taaaggttgt gcccgtcaac
    29881 agctttttca tccgctgact atatcccgca cttagctcaa cttctccagt aaccgagagt
    29941 gccatttgtg tctgttccat agctgcatcg attgttttat cttcttcttg aaacagaatc
    30001 agtgtttgca aaaaggtgtt cacttgctgt gtaacaactg ttttagaaaa ggcatctggg
    30061 cgactctgta tcttgatcgt cggcatcttt cctacttcga cttgttcggt aaagtctgca
    30121 ggcagatgta agataatgtt tacacgatta aaataaagag catcatcgat ttctttttgt
    30181 gaggttccat ccaatgtaac gactgtttcc ttgtcattca aatatttacg aaggccatcg
    30241 actacaggcg actcttcttc actaatcaag gcgattttcg catcttgcag agaggtattt
    30301 tcaacatcaa tattttgcgc aaaaaagaat gtggcaacaa cagtgataat tacacctatc
    30361 agtaaattcg atttatttgc tttaagcaat gtccaaatcg ttttataaag cgtcataacg
    30421 cacccctctt tccatccaag catttcctaa cgcaaaaagg aaaataaatc cgatcaacca
    30481 tagaagattg gtataaaaac tagccaaatc agtgtagaaa taaagtttat acaaactttc
    30541 gctgatcaag ttgacgagat ttaattgacc aacaatcgga atatgaagat caatccaata
    30601 cttcacttgt tccgacccca tcataccagc aaaaaagctc atcgcaatcg tcactgaaat
    30661 caagatgcta tccttttgca ccagattcat ttttggtatc aaattaccaa ccaatgtacc
    30721 taacaaaatc gcacacaacg accccagagc ggtaacaatc atcaaccatt gccaacgttc
    30781 accaaagtct acttgataga taaaacgaaa gacagctaac ataatataaa tctctgtcaa
    30841 aacaatcgta aagcttgctg ccaaattagc gattgaaact aacgattgct ttctaggaat
    30901 caaggacaag cgaataccat ttgacgattg atttgcttgc tgatcctgta cattgcggac
    30961 cccccacatc gtcccgtaga gaatcgccat tccaatcaga gtgaagaaat aaaaactttt
    31021 gaaactcata ttacgacctg catgaacttc ttgaaaagat acttcttgtt caaaaacagt
    31081 catgatgggc gcgagatcaa cgttctccac ttctgccact ttactcgaaa ttataccaat
    31141 acgctgtaaa aattgatcca tgacgttttt catcaacgtc tgactcattc ctgcttgacc
    31201 cacatggagc gaaacagatg aaccatcaat gagataaaaa ccagccaaat catctgcatt
    31261 tagcttttca ttcgcttcta cttccgatag ttcctgtact tcaaataatg gtttatcatt
    31321 gacttcgact tgttctaaga ctgtggtaaa attttccgaa agcgctgttt gatccgcttt
    31381 gattacaatt ccagtattaa tcgtttcgag attgccacct tgatcaatct cgccaaaggc
    31441 cgccataaac atcaatccta aaagaattgg aaaggccaat gtccaaaaca acatggattt
    31501 atttcgcagc agtatttttg tgcgatattt ataaaaatgc caaaacatta tttcccctcc
    31561 tctagtctct caaatccttg ccagtaatct ctaaaaagac atcattcaag gacgcttctt
    31621 tggttgctat ttttccgtaa ctgatctttt cttcttgtaa gattgtaaga atcggcaaca
    31681 atactgtatt tagttcagta gttgttactt ggagctgacc ttttttataa gcgattccat
    31741 cagaagctat tgttcgctga caacacgttt tctgggagta atgggatgtc gatcgtcaaa
    31801 cgatccgccg tccgtaccat atcttttaga gattctttgg ttcctttagc aatcacctgt
    31861 ccttgatcca tgatcacgat ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt
    31921 gtatagacaa tggttgctcc ttgtcgattc agctccttga tactttctaa aattttattc
    31981 cgactttggg ggtctacagc tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg
    32041 gcaatcccac atgcgatgtt caaccgacgt ttcaagccac cactaagttc tcctggacga
    32101 aacgaaacat atttttctaa tcccactaat tgaatcacct cttcaatcaa ggctttcctt
    32161 ttctcttttt cggaaacata taaaccgcaa aaataatcga tattttcttg cacacttagt
    32221 tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca ttgccttgac agcataattt
    32281 tcaggagtca ttgttgtccc gaacaattta atggttcctt tgtcataatt taacaaagaa
    32341 agcatacagt tgatggcagt cgatttccca ctgccgtttg gtcctaacag acctaagact
    32401 tctccttctt gtacttgcag atcaaaatga ttcaatgcta caaaatctcc atagcgtttg
    32461 actaatttat caatttcgat aatcatcatg aaaaactcct tccaaatttc ttgttgatct
    32521 gactataata aagtgcaaga aaagaaacta gtgacgattg gctaaaccac acatgacatt
    32581 tgtcacaatg aaggagtctg ctatgcataa atctcggcta ctcaacgaaa ctagctttct
    32641 actggttatc tcattgcttt tgctggtaag tcaaggattc gttgctatgc aagttatcta
    32701 tttattgatc gccgttttat tcactggtgt cagttattta ctgcctacaa agccaaattg
    32761 gattttccaa gttattctat ttcttttatg tgccttttta cctgatcttg tcctgtttct
    32821 gccagcgacg atgcgagtga tcgcccctga agaaagccac cttgattact tgtttttgtt
    32881 aggttttttg ttgatttcga cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt
    32941 aattttgctg tttttttcct tttacttacg aaaaaaagat caacgaacga ccgatcttga
    33001 gaaaaagtac attgtctcca aagatactca ttgggaaaaa caacaagagt tatcggaaca
    33061 aaataaagca ttggccctgt cacaagaaac acaattagcc ttgcaaattg tggaagaacg
    33121 gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt
    33181 acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa
    33241 tttaagtgcc accgttcaca ctgggatgga tcggattcgt caaagtgtcc atgacctcca
    33301 tgagacatcg atctcttttc aacactcctt agagtttctg atcgctgatt ttccttcttc
    33361 tgttacgatt gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa actgcttctt
    33421 aatggtcatc aaagaagcct taaccaacag tatcaaacat agtcatgcca aaaccgttgt
    33481 tttacatttt cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg atggcaccag
    33541 cccacaaaaa aattctgctc ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga
    33601 acaaaaggga caagtccata ttttccaaga aaaccagcaa tttcagttaa cgatcatttt
    33661 accaagggag ggacaccaat gataacagta gtcattattg agatgatcca tttgtgacga
    33721 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
    33781 gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca
    33841 tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca
    33901 aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt
    33961 ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca
    34021 aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa
    34081 tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc
    34141 ttgcaatcat caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt
    34201 acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac
    34261 gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca
    34321 caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca
    34381 attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg
    34441 attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc
    34501 tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc
    34561 tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa
    34621 tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata
    34681 tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct
    34741 ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat
    34801 catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac
    34861 agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc
    34921 cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag
    34981 cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc
    35041 gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt
    35101 atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt
    35161 gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt
    35221 gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat
    35281 cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt
    35341 ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac
    35401 tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa
    35461 tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt
    35521 gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat
    35581 tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc
    35641 ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat
    35701 ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg
    35761 cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg
    35821 ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac
    35881 ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt
    35941 cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc
    36001 aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc
    36061 attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg
    36121 tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt
    36181 tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac
    36241 agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc
    36301 agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg
    36361 aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg
    36421 ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt
    36481 ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc
    36541 aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt
    36601 ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat
    36661 agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc
    36721 tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat
    36781 gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa
    36841 cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc
    36901 agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc
    36961 agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt
    37021 cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt
    37081 tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac
    37141 taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac
    37201 tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt
    37261 gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat
    37321 ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt
    37381 cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag
    37441 gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact
    37501 cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta
    37561 cgaataaaag tgggccatct ttatgttggg caaccgcatc tgctacaaca gtttcgatac
    37621 gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat
    37681 gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg atcgactgac
    37741 cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac
    37801 gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat
    37861 aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac
    37921 tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt
    37981 gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt
    38041 attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt
    38101 ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg
    38161 ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt
    38221 tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct
    38281 cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat
    38341 gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat
    38401 ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac
    38461 ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc
    38521 cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc
    38581 tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat
    38641 caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta
    38701 tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct
    38761 caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt
    38821 ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct
    38881 ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt
    38941 tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca
    39001 gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa
    39061 aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca
    39121 tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc
    39181 gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct
    39241 tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca
    39301 ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca
    39361 tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac
    39421 atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta
    39481 accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt
    39541 ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac
    39601 agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc
    39661 cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat
    39721 acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg
    39781 ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt
    39841 gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa
    39901 gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact
    39961 tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat
    40021 tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg
    40081 ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg
    40141 acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg
    40201 accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc
    40261 ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca
    40321 ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca
    40381 aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg
    40441 cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt
    40501 gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac
    40561 tgctggatca aatcctagac gatccattag catcgggatt aaactacctg caagattcgc
    40621 cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt
    40681 ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt
    40741 caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag
    40801 tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag
    40861 cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa
    40921 agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc
    40981 tgcctttact ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc
    41041 gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa
    41101 atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc
    41161 cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga
    41221 aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg
    41281 accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt
    41341 cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt
    41401 catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc
    41461 gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa
    41521 gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat
    41581 ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc
    41641 taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg
    41701 gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat
    41761 attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc
    41821 atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct
    41881 gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga
    41941 tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca
    42001 tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct
    42061 gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca
    42121 gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg
    42181 gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag
    42241 gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca
    42301 aatccatgct ttgcaaaaag catcaaacca gatgtatccc gatctagtcg cgtcaccaca
    42361 tggattactt ggttttcgta accttgtttt ttataataag cctttacacg attagccatt
    42421 gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc
    42481 agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca
    42541 tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag
    42601 acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac
    42661 agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc
    42721 tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg
    42781 ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata
    42841 aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc
    42901 tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa
    42961 agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact
    43021 aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga
    43081 aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc
    43141 taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc
    43201 cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc
    43261 ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta
    43321 aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta
    43381 gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc
    43441 atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc
    43501 tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt
    43561 cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg
    43621 agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact
    43681 gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt
    43741 gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa
    43801 tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca
    43861 ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga
    43921 gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa
    43981 actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg
    44041 gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga
    44101 caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt
    44161 tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt
    44221 ccatagctga tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt
    44281 tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa
    44341 aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc
    44401 aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga
    44461 ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga
    44521 tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc
    44581 tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc
    44641 tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat
    44701 taatccaaac cggaaagctt acaacaaaac gggctgaatt ccctattgaa gaaggacttt
    44761 tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtaa
    44821 ggggatgcat ctgtgggaaa aattgctttt ataaattttt taaaacgttt ttctttacct
    44881 tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta gaaacctttc
    44941 attttcacaa ggcacttgag ttcatttttt tacttttttc tgttaagtta taggatgtga
    45001 aagaatctag tcactagttg tatagaatct attttggggg agattcatat gatcgaaatc
    45061 tatttatttg ttaatccttt aggggaatac tgtttcgaaa tggaacaaca gttattacaa
    45121 tttatcgaag aagagtacgg gacaccatca aaagaaaaga tgcaatttcg ctttcttcct
    45181 ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa aaggaatttc tcagaacaat
    45241 cttgaggaac gtaatcactt attctcaatg acttattctg cagcattgga ctgcaaagcg
    45301 gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga tcaaattgca agaagcagtc
    45361 ggctgtaatg acataccata ttctcaagaa cttgctgaat ccatatttga agaaatcggc
    45421 ggcgatttga atatgtttaa agaagatcgt cagtctgact tcgttaaaga agtattcttt
    45481 tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac acccttctgc agtcgtttac
    45541 aattatgcct gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac aagtagcctt
    45601 tggagaatca cagagctgtg taaaacaaca gataactcat atcaagtttt taatggtgaa
    45661 catttacata caaatgattc tcagttccat tcaagaacta gccatttaca cttgctttct
    45721 aattaaaaaa atacgtcgac tcccttacaa gagagccgac gtattttttt cgtttctatt
    45781 cgagaaaatc aaaaatagaa agccttataa acgatcgatc aattcttcta attcatttag
    45841 acgttgttca aacatcgaca ttgcatcttc aatatatgca gcctgtgtca tatcaactcc
    45901 tgcttttttc attacttcca caggataatc gctgttacct gcttttaggt aagccaagta
    45961 gttctctagt gcctctggtt cctggtttag tattttcttc gccagtgctg aagcagcaga
    46021 aaagccagtc gagtattgga aaacatagta attataataa aaatgcggta tccgtgacca
    46081 ttcgaattta atttctgggt cttcttcgac tgctggacca taatattttg cattcaattt
    46141 accataacta tcacttaggt attcactagt caatggcaca cctttttcat cttccgtatg
    46201 catgaaatgc tcgaattcag cgaattgtgt ctggcggaaa actgttcctt taaacccatc
    46261 caagtaatgg ttgagtacat aagcccgtac acgagggtct ttttctgttt ccaataaata
    46321 ctccgttagg atattttcat tcgttgtcga agcaatttct gctaaaaaga tggagtagtc
    46381 gccgtacaca taaggctggt tcgaacgagt gaaataacta tgaacactat gtcccatttc
    46441 gtggacaagc gtaaatagct gatccagcgt atcatgccaa ttcaataaaa tatatggatt
    46501 tgtgtcatag cttcccgaag aataagcacc gcttcgtttc cctttatttt cgacaacatc
    46561 gatccaacgt tcagagaatg ctttttctac gatggccatg tattcttcac ccattggttt
    46621 cagtgcttct aaagcttttt cttttgcttc ttcgtacgta aaggtaattg gagcttcccc
    46681 taagaccggt gtataaagat catacatgtg cagtttttcc acttctaata aacgtttccg
    46741 taattccatg tatcgatgta acaaaggcaa atgcttgttt accacgtcta ccaaagtatc
    46801 gtatacactt tcaggaatat gattattgct caaagacgct tctctggcag agctgtaatt
    46861 acggactttc gctttaaaat tatgtccttt tatatgtgtg cctaaagtag aagcaaatgt
    46921 atttctaaat tgttcgtaaa cactgtacaa tcccttaaat gctgcttcgc gcacccttcg
    46981 atccgtgctt tctagcaact gaccatacac gccatgagat aattggacta tttcaccatt
    47041 ttctccttca atcgttggaa aaactagatc tgcattattc aaaacagcaa atgtatcact
    47101 tgaagcatca aagatttcac ctgctccagc aagaagagat tcctgctcag cagataagac
    47161 atgggctcga ttatctacta tttgctggat ataatggcga tagacttcca attttggttc
    47221 ttctttgaaa tattgccaaa tctggtcatc tgacaattgc aatatttctg gttcaaacca
    47281 ggaaacggct tcactaactt tcgaaaacag actgcttgct cttgcataaa gcgcttggta
    47341 atctgtattt ccagtgtctt gatcgttttt aaggtgcgca tatacataaa tgacttcagt
    47401 ttggcgataa accctcaata cgaattcaat cgcatttaaa aattgagaag cgccttgatc
    47461 aagtgttcct ttgtgttttt cagattgttt taactcttct gataattcca aatatttctc
    47521 atcaaactct tggtcgcttg aaaagatctt ggtcaagtcc caagttaaat tttcaggcaa
    47581 ttcttctcgt tttggcaact gctttacttc catgaaatcc actccttttc ttcccttagc
    47641 tctatctatc aaaatagatc atagtctaac atccacatgt cacagcatct aacttttcta
    47701 ctcattcatg tgtctgcttc catcaatttt agcataacta aatcttgttt tacatagaaa
    47761 accgattcct agcctatttt tatacttact attttttcaa agaagacaag tgtatgcatt
    47821 cgttaaaaaa ctgctgatag accatctctt catcaatcat aggaaaaagc cattcccttt
    47881 tatgttcttt tatttggcaa agccatgagt tgaaatcagg aactttctgg ttttccaagg
    47941 cttcttcgta caacattcga taaagaaaaa cctgttcttg taaataaaag aaaaaccgac
    48001 ttttttgata gatccacgat gatagatgca aaagatgttt atgacgcaag tagcattgtt
    48061 cttgtatgga tactgtattc ttttgcttgt gaataagttg gcgtgtcagc catttcttcc
    48121 catcttcact cactttttct atttttttca tttggttcat tttaatttcg ttactagtga
    48181 atatttcttt aaggtcccta ctagaaaaag tccagcagct actgcaaaag gaaatatgcc
    48241 ctgatacagt ctctgtaata tgatgataca aataaagctt ttgtttcttg agatcagctt
    48301 tccagcaatg cactcctctt tttctattat agtagcagca acttttttct atcaatgaga
    48361 attgcgattg tccaagaaaa gaattaccaa gtatccacca tacttggtaa ctgttattca
    48421 aataattttg ggttctttca ataaatctct ggtgagacag tctagaacat tggatttcta
    48481 ttgctagatt cgccaacaaa atatccggcc tttgccgcaa cctcggcaaa taggcttcca
    48541 aaaatactgg ttctactgac tgtcccagcc aatccatgaa gcattccttt aactgtaagt
    48601 gctcttttga ttctctttca ctaaagaccg aacagttatc tgatttttga tgggcaaaat
    48661 gagcacattt tttctttccc tttttcaaaa tcactctttt tcgacagcca cgacaataat
    48721 atttctttcc tttttctgca tgtttagcaa aacagcactt attatattca ttcattgcaa
    48781 gcagcatttt tctccctctg aacttattta cgtctaagaa tttatttaca cgaaaaaaga
    48841 gggcgtgaaa aagctattct gcatcaagta ataagaacag ctaaacaaaa atagttccca
    48901 atatttttcg tttggttggg cttgttaccg cagatgcaag cttttgaaca catttatacg
    48961 aaaaaaagac tgcgacacaa tttttgtcac agtctcttac tataatgctg atttatacaa
    49021 aataaaattt cgttcgctcg atagcattac gcgccatcag acattgtcca tattcattca
    49081 atgtttctgg tgtacgatcg ctcttttcag caaactccaa aattcgagca atctgattct
    49141 ctacatccgc ttggttgaga ttttccatcc agaagtgaac gactaggtaa tagcgctctt
    49201 cgaataaata caaatcagtc caagcagact ctagttcgac atctgttgca agttggatca
    49261 cttgttcaaa atcttcaaaa gcaaaagttg cttgtctttc ctgttcttct agggcgcttc
    49321 cagataattc ttcctccgga tcgctgccaa taatctgctg acgaagaaag tcactcacat
    49381 cttctgaatc aacagtagaa tcttctgatt gagaaatttc ttcatccgga agatttttac
    49441 tgataaacag ttctaatccg tctccttttg gcaaaacttg gaaagtgaca gcttcgcttc
    49501 ctcggaactc ctcttcgata tcaacttctt ccaaaatact ataaaagaaa ttttcgattt
    49561 ctttgtgatt gccaagtaaa tccaaaaatg aaacgccccg ttcctctaaa tcttcatgac
    49621 caattagtac acggatcgta ttttcattaa tatgttccat ttccattttg ctacacctca
    49681 ctttgccaca atgtttaata tagattcatt gtagcgaaat cagcttaaat gtaaagaaaa
    49741 aaaatctcgc tgttaagcga atttaataaa accagcggat ttttcaacct ttcaagctat
    49801 cttatttaac tataacattt ttggcagacg aatactatat agccgacttt tcagctaatt
    49861 ttccctctct atttttcgtg caatcatcat cttctactac ctttttatac atctaatcaa
    49921 gaatactttt tgtaaaaaca aaaaaacttg gaaaaactat ctttgtaata gtcttt
//
LOCUS       DAZKOB010000009        47886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000009 DAZKOB010000000
VERSION     DAZKOB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.009"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            114..485
                     /locus_tag="ACG8M6_000538"
     CDS             114..485
                     /locus_tag="ACG8M6_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0456230.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(759..1451)
                     /locus_tag="ACG8M6_000539"
     CDS             complement(759..1451)
                     /locus_tag="ACG8M6_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0456231.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1454..2239)
                     /locus_tag="ACG8M6_000540"
     CDS             complement(1454..2239)
                     /locus_tag="ACG8M6_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0456232.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2476..3876
                     /gene="lacG"
                     /locus_tag="ACG8M6_000541"
     CDS             2476..3876
                     /gene="lacG"
                     /locus_tag="ACG8M6_000541"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0456233.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3901..4218
                     /locus_tag="ACG8M6_000542"
     CDS             3901..4218
                     /locus_tag="ACG8M6_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0456234.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4231..5904
                     /locus_tag="ACG8M6_000543"
     CDS             4231..5904
                     /locus_tag="ACG8M6_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0456235.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5918..7549
                     /locus_tag="ACG8M6_000544"
     CDS             5918..7549
                     /locus_tag="ACG8M6_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0456236.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8040..9605)
                     /gene="guaA"
                     /locus_tag="ACG8M6_000545"
     CDS             complement(8040..9605)
                     /gene="guaA"
                     /locus_tag="ACG8M6_000545"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0456237.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9664..10587)
                     /gene="coaA"
                     /locus_tag="ACG8M6_000546"
     CDS             complement(9664..10587)
                     /gene="coaA"
                     /locus_tag="ACG8M6_000546"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0456238.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10783..11388
                     /locus_tag="ACG8M6_000547"
     CDS             10783..11388
                     /locus_tag="ACG8M6_000547"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0456239.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11696..12712
                     /locus_tag="ACG8M6_000548"
     CDS             11696..12712
                     /locus_tag="ACG8M6_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0456240.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12853..14154
                     /locus_tag="ACG8M6_000549"
     CDS             12853..14154
                     /locus_tag="ACG8M6_000549"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0456241.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14200..14862
                     /gene="deoC"
                     /locus_tag="ACG8M6_000550"
     CDS             14200..14862
                     /gene="deoC"
                     /locus_tag="ACG8M6_000550"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0456242.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14883..15281
                     /locus_tag="ACG8M6_000551"
     CDS             14883..15281
                     /locus_tag="ACG8M6_000551"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0456243.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15347..16429
                     /locus_tag="ACG8M6_000552"
     CDS             15347..16429
                     /locus_tag="ACG8M6_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0456244.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16641..18203
                     /locus_tag="ACG8M6_000553"
     CDS             16641..18203
                     /locus_tag="ACG8M6_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456245.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18196..19329
                     /locus_tag="ACG8M6_000554"
     CDS             18196..19329
                     /locus_tag="ACG8M6_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456246.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19329..20285
                     /locus_tag="ACG8M6_000555"
     CDS             19329..20285
                     /locus_tag="ACG8M6_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456247.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20384..21550
                     /gene="deoB"
                     /locus_tag="ACG8M6_000556"
     CDS             20384..21550
                     /gene="deoB"
                     /locus_tag="ACG8M6_000556"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0456248.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21574..22392
                     /locus_tag="ACG8M6_000557"
     CDS             21574..22392
                     /locus_tag="ACG8M6_000557"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0456249.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22409..23116
                     /gene="deoD"
                     /locus_tag="ACG8M6_000558"
     CDS             22409..23116
                     /gene="deoD"
                     /locus_tag="ACG8M6_000558"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0456250.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23414..24100)
                     /gene="gpmA"
                     /locus_tag="ACG8M6_000559"
     CDS             complement(23414..24100)
                     /gene="gpmA"
                     /locus_tag="ACG8M6_000559"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0456251.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24272..24853)
                     /locus_tag="ACG8M6_000560"
     CDS             complement(24272..24853)
                     /locus_tag="ACG8M6_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0456252.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(24986..25669)
                     /gene="rpiA"
                     /locus_tag="ACG8M6_000561"
     CDS             complement(24986..25669)
                     /gene="rpiA"
                     /locus_tag="ACG8M6_000561"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0456253.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            25988..26401
                     /gene="rpsL"
                     /locus_tag="ACG8M6_000562"
     CDS             25988..26401
                     /gene="rpsL"
                     /locus_tag="ACG8M6_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0456254.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26485..26955
                     /gene="rpsG"
                     /locus_tag="ACG8M6_000563"
     CDS             26485..26955
                     /gene="rpsG"
                     /locus_tag="ACG8M6_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0456255.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27049..29136
                     /gene="fusA"
                     /locus_tag="ACG8M6_000564"
     CDS             27049..29136
                     /gene="fusA"
                     /locus_tag="ACG8M6_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0456256.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29308..30495
                     /gene="tuf"
                     /locus_tag="ACG8M6_000565"
     CDS             29308..30495
                     /gene="tuf"
                     /locus_tag="ACG8M6_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0456257.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30902..31210
                     /gene="rpsJ"
                     /locus_tag="ACG8M6_000566"
     CDS             30902..31210
                     /gene="rpsJ"
                     /locus_tag="ACG8M6_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0456258.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31241..31870
                     /gene="rplC"
                     /locus_tag="ACG8M6_000567"
     CDS             31241..31870
                     /gene="rplC"
                     /locus_tag="ACG8M6_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0456259.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31898..32521
                     /gene="rplD"
                     /locus_tag="ACG8M6_000568"
     CDS             31898..32521
                     /gene="rplD"
                     /locus_tag="ACG8M6_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0456260.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32521..32811
                     /gene="rplW"
                     /locus_tag="ACG8M6_000569"
     CDS             32521..32811
                     /gene="rplW"
                     /locus_tag="ACG8M6_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0456261.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32850..33683
                     /gene="rplB"
                     /locus_tag="ACG8M6_000570"
     CDS             32850..33683
                     /gene="rplB"
                     /locus_tag="ACG8M6_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0456262.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33726..34004
                     /gene="rpsS"
                     /locus_tag="ACG8M6_000571"
     CDS             33726..34004
                     /gene="rpsS"
                     /locus_tag="ACG8M6_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0456263.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34027..34374
                     /gene="rplV"
                     /locus_tag="ACG8M6_000572"
     CDS             34027..34374
                     /gene="rplV"
                     /locus_tag="ACG8M6_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0456264.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34388..35044
                     /gene="rpsC"
                     /locus_tag="ACG8M6_000573"
     CDS             34388..35044
                     /gene="rpsC"
                     /locus_tag="ACG8M6_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0456265.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35047..35481
                     /gene="rplP"
                     /locus_tag="ACG8M6_000574"
     CDS             35047..35481
                     /gene="rplP"
                     /locus_tag="ACG8M6_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0456266.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35471..35659
                     /gene="rpmC"
                     /locus_tag="ACG8M6_000575"
     CDS             35471..35659
                     /gene="rpmC"
                     /locus_tag="ACG8M6_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0456267.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35684..35950
                     /gene="rpsQ"
                     /locus_tag="ACG8M6_000576"
     CDS             35684..35950
                     /gene="rpsQ"
                     /locus_tag="ACG8M6_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0456268.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36008..36376
                     /gene="rplN"
                     /locus_tag="ACG8M6_000577"
     CDS             36008..36376
                     /gene="rplN"
                     /locus_tag="ACG8M6_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0456269.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36413..36721
                     /gene="rplX"
                     /locus_tag="ACG8M6_000578"
     CDS             36413..36721
                     /gene="rplX"
                     /locus_tag="ACG8M6_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0456270.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36749..37288
                     /gene="rplE"
                     /locus_tag="ACG8M6_000579"
     CDS             36749..37288
                     /gene="rplE"
                     /locus_tag="ACG8M6_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0456271.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37307..37492
                     /locus_tag="ACG8M6_000580"
     CDS             37307..37492
                     /locus_tag="ACG8M6_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0456272.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37528..37926
                     /gene="rpsH"
                     /locus_tag="ACG8M6_000581"
     CDS             37528..37926
                     /gene="rpsH"
                     /locus_tag="ACG8M6_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0456273.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            37958..38494
                     /gene="rplF"
                     /locus_tag="ACG8M6_000582"
     CDS             37958..38494
                     /gene="rplF"
                     /locus_tag="ACG8M6_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0456274.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38655..39011
                     /gene="rplR"
                     /locus_tag="ACG8M6_000583"
     CDS             38655..39011
                     /gene="rplR"
                     /locus_tag="ACG8M6_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0456275.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39032..39532
                     /gene="rpsE"
                     /locus_tag="ACG8M6_000584"
     CDS             39032..39532
                     /gene="rpsE"
                     /locus_tag="ACG8M6_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0456276.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39547..39726
                     /gene="rpmD"
                     /locus_tag="ACG8M6_000585"
     CDS             39547..39726
                     /gene="rpmD"
                     /locus_tag="ACG8M6_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0456277.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39770..40210
                     /gene="rplO"
                     /locus_tag="ACG8M6_000586"
     CDS             39770..40210
                     /gene="rplO"
                     /locus_tag="ACG8M6_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0456278.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40210..41505
                     /gene="secY"
                     /locus_tag="ACG8M6_000587"
     CDS             40210..41505
                     /gene="secY"
                     /locus_tag="ACG8M6_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0456279.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41567..42214
                     /locus_tag="ACG8M6_000588"
     CDS             41567..42214
                     /locus_tag="ACG8M6_000588"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0456280.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42405..42623
                     /gene="infA"
                     /locus_tag="ACG8M6_000589"
     CDS             42405..42623
                     /gene="infA"
                     /locus_tag="ACG8M6_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0456281.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42656..42772
                     /gene="rpmJ"
                     /locus_tag="ACG8M6_000590"
     CDS             42656..42772
                     /gene="rpmJ"
                     /locus_tag="ACG8M6_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0456282.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42790..43155
                     /gene="rpsM"
                     /locus_tag="ACG8M6_000591"
     CDS             42790..43155
                     /gene="rpsM"
                     /locus_tag="ACG8M6_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0456283.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43183..43572
                     /gene="rpsK"
                     /locus_tag="ACG8M6_000592"
     CDS             43183..43572
                     /gene="rpsK"
                     /locus_tag="ACG8M6_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0456284.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43652..44590
                     /locus_tag="ACG8M6_000593"
     CDS             43652..44590
                     /locus_tag="ACG8M6_000593"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0456285.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44618..44998
                     /gene="rplQ"
                     /locus_tag="ACG8M6_000594"
     CDS             44618..44998
                     /gene="rplQ"
                     /locus_tag="ACG8M6_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0456286.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45270..46109
                     /locus_tag="ACG8M6_000595"
     CDS             45270..46109
                     /locus_tag="ACG8M6_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0456287.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46085..46954
                     /locus_tag="ACG8M6_000596"
     CDS             46085..46954
                     /locus_tag="ACG8M6_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0456288.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46944..47741
                     /locus_tag="ACG8M6_000597"
     CDS             46944..47741
                     /locus_tag="ACG8M6_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0456289.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        15140 a         8763 c        10502 g        13481 t
ORIGIN      
        1 taatagattt aataagtact gataatgaaa tttctcctgg ttgggaaaac gaagttggtt
       61 accctgtaga agaatttttt caagattact ttgcggagta tgccgatagg ataatgagag
      121 attacgtaac agataaaact caagaagtca tcaatcaatc ccagtcatta aatgaattat
      181 ctgataaggc gaaagaagaa gcagttgcca ttatttcttc agggattagt gactatattg
      241 atatgagtgg tgaaatcaat tatggcgata aagacaaaat tgtacgcatg ataaaagctt
      301 ttatcaaaac ttttgatgtg aaggcagatc ttaagtttaa tgatacttac ctagtaggaa
      361 aattaattaa tatccttggg acagagaaag atacggaaac gtttatttcc tctttgtcta
      421 tgttgtttac agataagcat ttttcaactc agcttggtgg agaggaacta gtggaaatct
      481 tttaaccatt acgcccagca ttaataaaag tatatcaaaa aagtactcct gatgattttt
      541 aagattggtt tgagaaataa tgtgaaaaca ctatttttaa tgttaattat agatagagat
      601 gaacgcgtca tatggttaaa agaatgaaaa agtcaaagat acactgctat atttcactaa
      661 ggatatagca gtgtatcttt catgtcgttt cactggcagt tttttataaa aggaggtgtg
      721 catttcagtt gcttttcttt ggtagttagt tgaatcgttt aagaattaac ggatgtggca
      781 gacaggtgct ctgtccaaaa accagtaaaa tctttgatgg ttccatcttt atttagctgt
      841 gcctgttcaa atactgtttt gtttttaccg aatggatcaa ttgcaataat tgttccgatc
      901 cccgcacgtt tggcagcaag taatcctgag taagcatctt cgataacaag acaatcagcc
      961 ggagctaagc tcagtttgtc tgccgcttta agaaaaatat ccggttgcgg tttcccaggg
     1021 aaagagccgt cgtcatagac aatcttgtcc cagtcaaacc agcgggctaa atcgaaaata
     1081 tcaaaataaa aagcaacatt ctctttgaca gtagccgtgg caatcgttaa tggaatctgt
     1141 tggtcagtca gctggtcaag agtcgcttca agcccttttg tcaactgaag ctcgttttca
     1201 ttttgcaagc aaagcatacg ataatagctt tctttttctt ctgacaatac ggcgatctca
     1261 gaggcggtca aatgctctga aatgaaatgt ttcaaaatct ccgtattcgt ccgaccatga
     1321 atatttttta aaatatcctc gtctgataaa ccatcttttg tatgatcatg tatcatatgc
     1381 agccaagcag cttcatgcag atgagagtcc aaaaacattg ttccgttgaa atcaaatata
     1441 atccctttca tagttacgct cctatagtgg tattttcggt tgaatcagca atttgcagat
     1501 cgacatattg tgcatagtgc tcttgaacct cccgcgagat ctggcgatcc gtaatgagca
     1561 gatcgaaatc atgcaaatta taaaacacat aaaaatcatc tcgattgaat tttttattat
     1621 caacgagtag atatttctta cgggaattat ttagagcgat ctgctgtgct tctccttctt
     1681 cggtactgta agtagagatc gcttcgttgt gaacgccatt tgaactgata aatgcttttg
     1741 taaactttag tcttcttaaa ttgtcgttgg tgatcggtcc gacaaatgcg ccggtatttt
     1801 tccggaaatc accgccaatc agtagaattt ctggtccatc tgcttgagca agaatatcaa
     1861 aaactggata attatttgta atgatccgaa ttttcttgtt cagtaaatgc gtcgccaata
     1921 gttcgattgt tgtacctggt cctaagaaaa tcgtttctcc ctcgtgaatc aaggaggctg
     1981 ctgtggcagc aatttctttt ttttcttgaa tctgcagagt ggctttttca gtgtgagaaa
     2041 gttcttgatc taaagtgtag gaaagacttt gagcccctcc atggacacgc agcagcttgc
     2101 ccgatttttc taattcatct aaatctctgc gaacggtcat atcagaaaca tctaattgat
     2161 tgatgatttc attaacggta aggattcctt ttttattgac catttctacg atagtaagca
     2221 atctttcttt tttcaacatt gtcttcacct ctaatttaag gataatctat catataaacg
     2281 gacaacagtc aatctaattc gaacaaaagt aaacatttag ggtggttttt atgtttgtta
     2341 caatagattt tattgctgtt ttctggtttt tatgattatt tgtgtttgtt tttgttgaca
     2401 aatgttttgg atgcgtttac aatgcaatca gttagaaaga gcgaaaactg attatagatt
     2461 tgaggagaga tgtacatgct taaattacca gaagatttta tttttggcgg agccacagca
     2521 gcctatcagg ttgaaggagc cactaaggaa ggtggcaaag gcgctgttgc ttgggatgac
     2581 tttttagagg aacaagggcg attcagtccc gatccggcaa gtgattttta tcatcaatat
     2641 gcaaaggaca ttgaattgtg tgaacgtttt ggtgtaaatg gtctaagatt atccattgct
     2701 tggagccgca tttttcctga tggagctggg aaaccaaatc cagaaggaat cgctttttat
     2761 catcgtgtgt tcgaagaatg taagaaaaga aacgtaacgc cgtttgtgac gttgcatcat
     2821 tttgataccc ctaaacgttt gtttgaccat ggagattttt tgaatcgaga aacaatagaa
     2881 gcatttgttt cctatgccat tttttgtttt catgaattca aagaagtcaa ggtgtggagt
     2941 acctttaatg aaatctatcc agttgcaacc aatcaatatt tgttaggtgt tttcccacca
     3001 ggaatcaaat atgattttac aaagatcgtc gcctgtcttc ataatatgat ggttgctcat
     3061 gcccgtgtag tgaactattt caaagaaaac gaacttccgg gtgaaattgg tgtagttcac
     3121 tcattagaga cgaaatatgc ggcaacagac gcgccggaag ataagcatgc tgccttttta
     3181 gacgatgcat tgtcgatccg tttcttatta gatgctacct atttaggcta ttattcaact
     3241 gaaaccttga ctgctcttga tgagatatgt gaagcaaatc aggccagcta tcattttcct
     3301 gaagaagatt tcgttgaatt gaaaaaagca tccacgcgta atgactactt agggatcaat
     3361 cactaccaat gccattttgt caaagcttac gatggagaaa atgcaatcca tcataacggt
     3421 acgggtgaaa aagggacctc tgtttataaa gtgaagggaa tcggagaacg tatttataaa
     3481 gaaggaattc ctcgaactga ttgggactgg ctgatttatc cagaaggtct atatgacttg
     3541 ttgctgcgaa ttaaaagcga ttacccacat tacaacaaaa tctatatcac tgagaacggg
     3601 atggggtata aagatcagtt tgaagatggc attattatgg atcagccgag aatcgactat
     3661 ttgagagtct atttggaatc cttaagcaag gcgataacag ccggggtaaa tgtgaagggg
     3721 tattttttgt ggtcgcttat ggatcttttc tcatggacaa atgggtataa caaaaggtat
     3781 ggcttgtttt atgttgattt tgaaacccag aagcgttacc caaaagaatc agcttactgg
     3841 tacaagttgg tcagtgaaac caaaacgatt atttaataag tgataatgga ggaagaaaag
     3901 atgaataaag aggaattaca aatgcttggt tttgagatcg ttgcatattc aggcgacgct
     3961 cgcagtacat tattgaaatt attgaaagaa gtaagacaag gaaactttga acatgttgat
     4021 tcggcgttga aagaagccga cgaaaattta acccttgcac ataattcgca aacaaaaatt
     4081 ttagcggaag aagcatctgg gaaagagatg gagatgggat tcatttttat ccatgggcag
     4141 gatcatttaa tgaccacttt attgctgaga gatctgattc aagactttat tgtactgtac
     4201 agacagaatc aaggatagga gaagaacgct atggatggaa tcattaaaca aatcgaaaaa
     4261 gggaaaccat tttttgaaaa aatctcacgt aatagttatt taggagccgt tcgtgacgga
     4321 tttttaacag cgatgccagc tattttattt tcaagcattt tcattttgat tgctgcggtt
     4381 cctgagattt ttggttacca atggcctgaa tctgtctcca catggctatg gaaagtctat
     4441 aattattcga tgggtgttgt agggttactc gtttcagcaa ccactgctcg ctgcttggct
     4501 gattcgatga atcgtcgctt accgaaaaac aaagtaatca acaccacctc tgtgatgctg
     4561 gcctctgtgg taagtttcct gttgttaagc gtctctccga ttgaaggtgg attttcaacg
     4621 gaatatatgg ggacaaaggg actactggct tcctttgtcg ctgcattttt aacagttaat
     4681 ttatataagt tttgtgtgct gaaagatatt accattaaaa tgcctaaaga agttcctgga
     4741 acgatttcgc aaaccttccg agatattttt cctttctctt tttcagtatt tgcggccgtt
     4801 ttaattgatt taggcattcg acaaatcttc caaatctcat ttgcagaagc aatcattacg
     4861 gtgctgcagc cgctatttac agcagctgac gggtacttag gaatcgctat tatctgggga
     4921 gcgatggcgc tgttttggtt tgtcggtgtt catggaccgt cgatcgtgga gccggcaatt
     4981 gcggcaatta tctatgccaa tgtggaaacc aatctgcaat tgttcaattc cggtgaacaa
     5041 gcttctaaag tactgacggt gggtttaggg aattttgtag gaactatggg aggaactggg
     5101 gcaacattgg ttgttccttt tatcttccta ctctttgcta agtctaagca gttgaaggca
     5161 gttgggaaag cttcctttat tccagtaagt ttcgctgtca acgaaccatt gctatttgcg
     5221 gcacccatga ttttgaaccc ttattttttt attccatttt tgttaacacc tgttgtaaac
     5281 gtttgcctgt tcaagttttt tgtcgatgta ttgggcatga acagctttat gtacgttttg
     5341 ccttgggcaa ctccagcacc gatcggtttg gttctaggaa cgggcatggg tatcttagct
     5401 tttgttctag cagcggttct aatcgtcgtt gacttcctga tctatctacc attttgcaaa
     5461 gcatacgatg ctgtcttagt agagaaagaa gcacaaaaag cagaggtgat cgcaaccgag
     5521 gaaggtgctt ctgccgatgt acgtgaggaa gtacctgtgg cagcgagtca agttatcagc
     5581 gatgcaaata aagaaatcaa tgtgttggta ctttgtgctg gcgcagggac aagtgggatg
     5641 ttagctaatg ctttaaaaaa aggtgcatct gaatcgaaag caccgatcag tgcaatggcg
     5701 ggtgcttatg gttctcatta cgaaatgctg caggattttg atatggtggt cttagcaccg
     5761 caagtcaatt cttactatga tgatatcaaa gaagacaccg atcgcttagg aatcaagctg
     5821 gttgcgacaa aaggagcaga atatatcgga ttaacgagag atcctgaaaa agctgttcag
     5881 tttattctga accaatttca ataaaggagg agccataatg aaaatgggaa tttggttgcg
     5941 taaagctgtt ttatttgcag ctgtgttagg ggctttgggg gctgtttcaa aaactgttga
     6001 agcatctgcc agcgaattta ttcgcggagc agatatttcg atcctgtcag atatggaaaa
     6061 atctggtgca aaatattatg aagacgatat ttcaaaagat gccttagaga ttttaaaaaa
     6121 taatggtgtc aactatgttc gactgcgttt gtggcaggat ccttataata gtaatggaga
     6181 aagttatgga gcagggacaa atgatctgga aacgacgatt gaccttgcaa aacgagcaaa
     6241 aaatctggga cttaaggtgc tgctggattt tcactacagt gatttttggg ttgatcctgg
     6301 gaaacagaat cttcctaaag catggcaagg tctgaccttt gaagaaatga atacggcatt
     6361 gtacgactat acaaagaacg ttctgtcgga aatgaagcag ctggacgttt atcctgatat
     6421 ggttcagatc ggcaatgaac tgaatagcgg aatgctctgg ccttatggca agtcttgggg
     6481 agaaggcggg ggagaatttg atcgattggc tgcttttttg aagtctggta tccaagctgt
     6541 tcgagacact caacctaaaa caactccagt aatgctccac ttagctgatg gtggcgatac
     6601 aggagcattt acttggtggt ttgatgaaat cacgagtcga ggtgtgtcat ttgatttaat
     6661 tggagtgtcg tattatccgt attggcacgg ttcaatgaat gaacttcaaa ccaatatgaa
     6721 tgcgatcagc caacggtatg gtaaagaagt gatcgtagtt gaaacagctt atgggcatac
     6781 aacagcgaat gccgatacta tgccaaatgc ttttggtgaa gccgaagcag ctgcgggggg
     6841 atatgaacca acaccgacag gacaagcaga gtatttgctg gatcttgccg atcggattca
     6901 ggcagtaccg aataatcgcg gggcgggctt cttctattgg gaacctcttt ggtacaacgg
     6961 aaatgtgagt tgggcaacac aagcaggtat gaattatctc agtgttcaat ccactatggg
     7021 aaacgaatgg gacaaccaag cgatgtttga ttttgctgga aacgccttgc caagcttgcg
     7081 tgcgtttaaa caagcagggg cgcaaataaa tctagttaaa aatgcaagtt tcgaaagcga
     7141 tggaccaacg accagtcctt ccaattggca aacctggttt caacaaggaa ccgctaaggg
     7201 tacgatcaaa acggaagcgg gcactttttt tggggactac aaactaactt tttggcagga
     7261 tcaagcatac gaggcatcag cttatcaaaa tgttagtggc ataagcaaag gaacttacac
     7321 attgagtgcc tatgtaatgt ctgggggaga gtttgatact aatcagttat atatcaaaaa
     7381 ttatggtgga gcggaactaa atactgcggt tgttaaaaca agtgaatgga caaaaattac
     7441 aatcaaagac attccaatta caacaggtgt ttgtgaaatt ggggtttata gcaaggcttc
     7501 aggaaatgct tggacgaacg ttgattttgt ttcattggtg aaacaataga ctgcggatcg
     7561 tttcgattca agatcggagg ttcccttcag ccacatcgtt agcgtagaag gcaggaatcg
     7621 aacctgcacc tcgcctttgc caaaaataac cagaaacgat ttccagtcat ttttaccaaa
     7681 acgagtaaag caccagactt catctacact ttgtattatg ctaaagagag ttcaacgatt
     7741 tcgtaagaag caatgaaaaa attgcaaaaa gttgtgcaga taacataaag tttgtttaag
     7801 aaagggtaac cttagaattc cgtcgtgaat atgatttgag cacttagatg gatagaaaaa
     7861 agttctcgga aaacagttcg tttttcgaga acttcttttt atgataagaa tgaaacaaat
     7921 taacttatat tatagtctct tttggtaggg aaccatctta tttttaatga aaatcaagca
     7981 ttttagctat tgaagaatgt tgatttatag ggattccccc tccatattat gttggctctt
     8041 tattcccact ccactgttgc tggtggttta gaagtgatgt catacacgat acggttgacg
     8101 tgggctactt cgtttacgat acgtacagag attttttgca agacttccca tgggatacgg
     8161 gcaaagtcag cggtcattcc gtcgattgaa gttacagcac ggataccgac tgtgtagtcg
     8221 taagtacgtc cgtcacccat aacaccgaca gagcggatcc ctggaagaac agtgaaatat
     8281 tgccagatat cacggtcaag tccagcagct gcgatttctt cacgcaagat agcatcagat
     8341 tcgcgtacga tttctagctt gtcttcagtg atttcgccaa gaacacgaat accaagacca
     8401 ggaccaggga atggctgacg ccaaacgatt gaatcaggca tgcctaactg agtccctaat
     8461 tcacggactt cgtctttgaa taatgtgttt aatggttcga tcaattcaaa ttgcatatct
     8521 tctggcagtc cacctacatt atggtgagat ttgatcgtct gtgctgtttc tgtaccagat
     8581 tcgataacgt cagtatacaa cgttccttgt gctaggaaag agacgccttc ttcaccagca
     8641 agttttgttg cttcgtcatc aaaaacatat acgaattcat tcccaatgat tttacgtttt
     8701 tgttctgggt cagatacacc tttcaacttg tctaagaagc gatctcttgc attgactttg
     8761 atgatgttca agccgaattt tccgcctaga ctttccatta cttgttccgc ttcgcctttg
     8821 cgtagcagac cgtggtcaac aaagatagat gtcaattggt caccgatcgc tttttgtaaa
     8881 agaaccccaa cgacactaga atcaacgcca cctgaaagac caagcaagac ttttttgtca
     8941 ccaacttgtt cacggatttt ttctacttgc atatcgataa agttatccat tgtccagtta
     9001 ccttcacagt gacaaacatc aaaggcaaag tggcgcagca attcgattcc gtattcggaa
     9061 tgacgtactt ctgcatggaa ttggatacca taaaaattac gtgttgtgtc ttggatagaa
     9121 gcaatcggac aatctttgct tgttccgact gtttcaaagc cttcaggaac tttagttacc
     9181 aagtcaccat ggctcatcca tactgtttgt tgttttggtg tttctttaaa caagacagct
     9241 tgatcgcttg ttacttctaa caaggctttc ccatattcgc ggtttttagc aggctctacg
     9301 gaaccgccaa gtttgtacgt gatcaattgc ataccatagc aaatccctaa tacaggaata
     9361 cctaaattga agatctcagg atcgatccca aatgcattct catcatatac actgtttggt
     9421 ccgccagaaa gaatgattcc ctttggattc atcgctttta cttcttctgc agtaatgcgg
     9481 tggctcaata attcggaaaa gactccaaga tcacgaatac gtcttgtgat caattggtta
     9541 tactgactac cgtagtctaa tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt
     9601 gtcaaattta ttacccctat ccctttaaat tttatctcaa aatagtcaaa ctattttaaa
     9661 atgctaatat tttcgcatga aaatcgaatc gatgatatga ttttcagttt tatggagaat
     9721 tatatcggct cttcctcgtg taggcaagat atattcctgt aaatttttca aattcacatt
     9781 tttccataca tcttttgcca ttttaaaggc ttcttcacga tctccgatag caaaacgata
     9841 ataataattc tctggatttt ggaaagccgt atccagtaac gcaccaaaac gatccaaata
     9901 ccatttttcg atcagcacgg gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct
     9961 gacgtagatt tgctgattcg caggtaattg aagcgtattg atcccctcaa cgatcaagat
    10021 atcaggctgc tggatcaatt cgtacttgtc tggaatgata tcatacacat catgtgagta
    10081 aacaggtact tttatctctt cctgaccaga ttttacacgg ttcaagaaat cgatcagcat
    10141 ctccatatca taactttcag gaaatccttt acggtccatg attccacgct cttttaatat
    10201 acgatttgga taaagaaatc cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt
    10261 acgagacaaa atcgtctgca gcagtctggc tgttgtactt tttccaacgg ctacacttcc
    10321 ggcgatcccg ataataaaag gcggtacttc tacgtattta tgaagaaata aacctttact
    10381 caagaacaaa gattcaaatt ctttcatata aagataaatc agatgagcaa gaggtacata
    10441 gatatctcga acatcctgca tggaaatgcg gtcattcaaa cttttgatat tatctagttc
    10501 atcttgtgtc aaaggtgctt gcccattacg ataaaattct cgccattctt ctcttgccac
    10561 acggtagtag ttcattggat cattcatatt agcctcctgc gttctcccat caatggaact
    10621 attttaacac aaaataaacg caaaccccac cacttaagag agattgccga tctttttgta
    10681 taatctttat aatagaaaga aacggagtct tattcttgac ttgcgcacgg atacataaaa
    10741 ggatagactg ttgtcatgaa agaaatgaga aggatggaag acatgacaaa tcattactat
    10801 tctgaacgcc cagatacggc gcacgatttt gaacaatggg cttttgagtt aaaaggcaga
    10861 acctttcaat ttgtaacaga ttctggtgta ttttcacgtg aaacagtgga ttatggttca
    10921 cgtgtcctga ttgatgcttt cgattgggaa gagttacctg atgaaggaaa gatcttagat
    10981 gtgggatgcg ggtatggacc gattgggtta gctttggcat ttgcgacaca gcggtttgtt
    11041 gaaatggtcg acattaatga acgagcggta agtctagcgc aaggaaacgc aaagcgtaac
    11101 caaatcgaac atgttgatat ccatcaatcg aatatctatg aagcagttca tgaagaaacc
    11161 tatgcagcta tcgtaagcaa tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt
    11221 ttgacaggcg catatcctcg gttgaagaag ggcggtacac tgacaattgt gatccagaag
    11281 aaacaaggag cgccgagtgc gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc
    11341 gtcacaaaag ataaaggata ttatatcatc aaaagtgtga aagaataact agctgaaaaa
    11401 tagtggagcg acgaaagagt ggaactgggc gtgcatgtac gggtacgtat cagttctttt
    11461 tttgttgaga agtttttaaa aacaaagaac tattttttct tcaaaaagaa atagtaggaa
    11521 cagagtcgtt gttttttacg ataaaataaa taagatacag aacttgtata gaaggctgcc
    11581 aagtcggtag ataaaggagg tttttatcaa aaaacatact ttcctttttt ctctattctt
    11641 ttcttggggg ttttggtaca ctgataggga atttagatag aagggagcca ataggatgaa
    11701 gctgaccatc agagatatcg cagaaatggc aggcgtatca gtaacaactg tctcacagat
    11761 tttgaataac aaaggaagca gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt
    11821 gaatgaacat cattataagc ccgattattt cgcttcaaat gtgatcaatc gacattcaaa
    11881 gaccatcgga atgatcgtcc ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg
    11941 tgtagagagc tatttgaatc ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag
    12001 ccaagaaaat gagatcaagt atttacaaga gctttcccac cgctcagtgg acggaatctt
    12061 gctggccaca ccaaatattt tacctgagaa atatgcatta gacagcggtt tttatcaaaa
    12121 aatgccaatc attttaatcg atcgcggatt gaatcgtcgg gataacggac ggcttatcgt
    12181 aaaggaatat gagggcgcct atcaagctgt ttcgctgttt attgaaaatg gccatacgaa
    12241 gatcggcatg ctgaaggaaa ccactggata ctatcaatta gaagaacgtt tcaacggata
    12301 tcgtcatgcg ctgaaagatc atggcctgcc ttttaatggg aaatttgttg aacatggaga
    12361 gttgactgtc caaggtggat atactgcatc aaaaaaatta ctcaaacata aagatatcac
    12421 ggctattttt tgcgggaatg atgccatggc gatcggatgc tatcaggcga tcgatgaatt
    12481 gggcaaaaaa atcccagaag atatctcagt cataggattc gatggattaa agctgtcaga
    12541 atacatgatt ccgcaattga cgacagtgca gcagccaagt tttgatattg gattttatgc
    12601 tgcacgtttc ctgatcgata cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt
    12661 cgaaacaaaa ttaattattc gtgagagcgt aaaagtattg acaaagggct aactcggcgg
    12721 tatattgctt atgtaagcat ttacaaaggc tagttagtgc tttttttatt tttagtaaac
    12781 cagtttacta aagtggttta cctttcctaa aaataatgta taataccgaa tgaagacaag
    12841 gaggatcagg caatgcgaat ggtggatctg attgaaaaaa agagggatgg aaacgagctg
    12901 tcgaaagagg aaatcgagta catcgtcacg aactatacga atggaaagat tccagattat
    12961 caagtcagtg cattattgat ggcaattttt tatcaagaca tgacaaatga agaaatcacg
    13021 aacttaacat tggcgattgc taattctggt gatgtcatcg atttgagttc tcttgaagga
    13081 ataaaggtag ataagcattc gacaggtggc gttggtgaca cgaccacatt gattttagcc
    13141 cccctagtag ctagtgtcgg agtgacagtg gctaaaatgt ctggacgcgg tctaggttac
    13201 acaggtggta cactagacaa gctagaagca attcccggat ttcagatcga gctttcggat
    13261 gaagaatttg tacgcattgt taatgaaagt aaagtagcgg taattggtca atcaggaaac
    13321 ttggcaccgg cggacaaaaa attgtatgcc ttgcgtgacg tcacagcgac cgttgattct
    13381 cttccgctga ttgccagttc gattatgagt aaaaaaatcg cggcaggagc agatgcaatc
    13441 gttttggatg tgaccactgg agacggtgct ttcatgaaga acatcgaaga tgcaagacgt
    13501 ctggcaaaga ccatgacgag cattgggaaa ctggctaacc gagaaacagt ggcggttatc
    13561 tcagatatgt cagaacctct tggcgaagct atcggaaata gtctagaagt tgtcgaggca
    13621 atcgaaacat tacaaggaaa tggtccagaa gatctagtag aaatgtgcta cgccttagga
    13681 agccaaatgg ttgttcttgc tggcaaagct aagacaaccg atgaagcaag aacgttgctt
    13741 caagaagctc tggaatcagg taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa
    13801 gggggagacc cagcgatcgt ggaacatcca gaacgaattt tgactgcacg ctacacaatg
    13861 gagcttcctg ctaaacaaag cggagttgtg tcaaaaatcg tggcaaatga attaggaatt
    13921 gctgcaatga tgctaggtgc tgggcgtaaa accaaagaag aagatattga tcacgctgtt
    13981 ggattgaaac tgcataaaaa aattggtgac actgtgacaa aaggtgagtc actattgacg
    14041 atctatagta acgacgaaga aatcacttct gtcattgaat tattatataa aaatattgaa
    14101 attggtgaat cggcaatgaa gccaacgttg attcatgaca tcatcacaga ataaaggaaa
    14161 aaagcggact ttaccgtttt aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc
    14221 gaccatacta ttttgaaagc agacgcatct aaagaagctg tgatgcaaat tatcgaagaa
    14281 gcaaaaaaat accattttta ttctgtctgc atcaatccag catgggttgc gctagcgaaa
    14341 gaacaattac aaggtgagcc agtagccgta tgtacagtaa tcgggtttcc tctaggagca
    14401 aacacacccg agacaaaagc atttgaaaca acagatgcca tcaataacgg tgcagatgaa
    14461 gtcgatatgg tcatcaatat cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa
    14521 gatatcgaag cagttgttga agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa
    14581 acatcgttat tagatagaga agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc
    14641 gctgattttg tcaaaacatc aactggtttc tcaacaggcg gagcaaaggt agaggatgtc
    14701 cgtttgatgc gtgaaactgt aggaccagaa atgggtgtca aagcttccgg cggtatccat
    14761 aacgaagaag aagcaatggc aatgatcgaa gctggtgcta caagaatcgg aacaagcgca
    14821 ggagtagcaa tcgtatctgg ttcaactggt gaaggctatt aattttttca aaggagaagc
    14881 tcatggcaga actcaaacaa gaatggatca acacagctat tgaagcgtta gataaagcct
    14941 atgttcctta ttcccatttt ccggtaggcg cttgtctcgt aacggaatca ggcaagattt
    15001 atcaaggcat caacattgag aatgcatcat ttggattaac gaattgtgcc gagcgcacag
    15061 cttttttcaa agcagtatct gaaggagaaa aaacatttac gcatctagtc gtagcggggc
    15121 acacccccga tcctatctct ccttgtggtg catgtcgaca agtaatggca gaattctgtt
    15181 cccctgatat gccggttacg ctagtaggag acaatggtgt gacgaaggca acgaccgttc
    15241 gtgcactgct gccttatgca tttactgaaa aagatctata actcatgttc attcagtcag
    15301 ctgactgttg aaaaataaaa caattatttt aggaggcttt acagaaatga aaaaagcaaa
    15361 attatttggt ttaggtgctg tcgcactagc agcagggtta tttttaggtg catgcggcaa
    15421 caacggctct actgattcat ccggtggcaa aagctcatca gacacaacaa ctgcagcatt
    15481 gatcacagac acaggtggtg tagacgaccg ttcattcaac cagtcagctt gggaaggact
    15541 agaaaaatgg gggaaagatc aaggactttc acgaggaaat gatggcttcc aatatttcca
    15601 atcttcaaat gaatcagatt acatcccaaa tattgaccaa gcattgaatg ctggattcaa
    15661 aacgattttt ggtattggtt ataaactaaa accagcgatc caagaacaag caacaaacaa
    15721 tacaggtacg aactttgtta ttatcgatga tgtcatcgat ggattagata acgttgtatc
    15781 tgcaactttc aaagacaacg aagcttctta tcttgcaggt gttgcagcag cttatacgac
    15841 tgaaacaaac gtagtcggct ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga
    15901 tgctggattc aaagccggtg ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt
    15961 attgaatcaa tatgctggtg acttctctgc tccagataaa ggacgttcaa ttgctcaagg
    16021 tatgtatgca caaaacgcag atatcatctt ccatgcttca ggcggtacag gtaacggtgt
    16081 attccaagaa gcaaaatcat tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt
    16141 tgaccgcgac caatcagacg aaggcgaata cacattaaac ggcgaaaaga aaaacttcac
    16201 attgacatct acactgaaag cagttggaac agtagtagaa gatttggcac aaaaatcagc
    16261 ggacggtaaa ttccctggcg gcgaacacac tgtttatgga ttgaaagaag acggtgttgg
    16321 tctaacagaa ggacaacttt ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa
    16381 aatcatttca ggtgacgtaa aagttccaga aactcctgaa gaaaactaaa accatttcta
    16441 tagccttgtt agacacatgt ttgacaaggc tatggtttga ttagaacaag aattgttcat
    16501 ttatccaagc tgccttgtga cagtttggat aagggaacaa ctccctgaag taatgtttgt
    16561 ttcaaaagaa gaacgctcac taagcgtgaa tcttagtcaa tcgttgccct agatagaaat
    16621 cgttaggatg tgaaaaaaag gtggttcaag aaaatattgt catagaaatg cagaatatta
    16681 ccaaagaatt tggaacattc aaggcaaatg acaacatcaa tttacaagta aaggcaggag
    16741 aaattcatgc gttgcttgga gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt
    16801 ccggattatt agagccgaca tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca
    16861 caagtccgac caaagccaac caattaggga ttgggatggt ccatcagcac tttatgcttg
    16921 ttgatgcctt tactgtaaca gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga
    16981 tgcttgacca taaaaaagca cgaaaagaga tccaaaaagt ttctgaacag tatggattgt
    17041 cagtcaaccc agatgcttac gttcgtgata tttcagttgg aatggaacaa cgggtagaaa
    17101 ttttaaaaac actttaccga ggagcagatg tactgatttt tgatgagccg acagctgtat
    17161 tgacccctca agaaattgat gaattaatcg tgatcatgaa ggaattagtc aaagaaggca
    17221 agtcgatcat tttgattacg cataagttag atgaaatcaa agcagtagct gaccgctgta
    17281 cagttatccg ccgtggaaaa ggaatcggta cagtcaacgt taaagacgta acctcacaac
    17341 aattagccga tatgatggtt ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga
    17401 agcctcaaga agtcgttttg tctattgaaa atctagtggt gaaagaaaat cgtggattag
    17461 aagccgtgaa aaacttgaac ttagaggttc gtgctggcga agtacttggt atcgctggaa
    17521 tcgatggaaa cgggcagtcg gagttgatcc aagctttgac tggtttgcga aaggcagaaa
    17581 gcggacatat caagctaaaa ggggaagaca tcactaataa aaaaccccga aaaatcactg
    17641 aacatggtgt aggacacgtg ccagaagacc gtcataaata cgggttagtc ctagatatga
    17701 cattatctga aaatatcgca ctacaaacct atcatcaaaa accttacagt aaaaacggca
    17761 tgctgaatta ttcagtaatg aatgaacatg ccagagaatt gatcgaagaa tatgatgttc
    17821 gaacaacgaa tgaacttgtt cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa
    17881 ttatcgctcg tgaagtcgac cgagatcctg atctgttgat tgttgcaaat ccaactcgtg
    17941 gactggatgt aggtgcgatc gaatttattc ataaacgtct gatcgaacaa agggacaaat
    18001 acaaagcagt gttactgatc agtttcgaat tagaagaaat tttaaatgtt tcggatcgta
    18061 ttgctgttat ccatgaagga gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa
    18121 atgaattagg tctattgatg gcgggctata ccttagaaga agcacgaaaa gagttagaaa
    18181 agggggctgg cgcacatgaa tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta
    18241 ctttcagttg ttttaggatt gatcttaggc gcgatattga tggctgcttt tggttttcag
    18301 cccatccaag gttattcttc tatgctagat gcagcccttg gttcgcaaag agggataggt
    18361 gaaacgttaa ggcaagcaac gccgttgatt tttacagcat taggtttttc tgtagctaac
    18421 tcagctgggt ttttcaatat tgggctttct ggacaagcac tttgcgggtg gatcgtcagt
    18481 gtctgggtgg cacttgcgtt tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg
    18541 atcttaggtg ccttagctgg tgcgatggca gcggctgttc caggtttact tcgagcattt
    18601 tttggaacaa gtgaagtgat cgtaacaatc atgatgaact atatcttatt atacgtaagc
    18661 acatttgctt tacatgaagt gatgcctgaa agctatcgct caagtttgga ctcttccaat
    18721 atgatcagtc aaaatgcctc actgcggtta ccatggttaa cacaagcttt tggcggctct
    18781 cgcgtaaact caggattatt tttagcaatc atcgcgctta ttttcgtttg gctgctgatg
    18841 aagaaaacga ctttaggatt tgaaatccgt tctgttggat tgaatccttt tgcttctgag
    18901 tatgcaggaa tgagtagtaa acgtacgatc gttttgtcga tggtcatttc tggcgctttg
    18961 gcagggcttg gcggtgtggt tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg
    19021 acttcgctat ctattggatt tgacggaatg gctgtttcct tgcttggttc tggatcagca
    19081 atcggtattc ttttatcagc gatcttgttc agtattttga agatcggtgg acttggcatg
    19141 cagacaggag caggtgttcc gtttgagatc gtcaatgtat cgattgcttt gattatcttc
    19201 tttgtcggga tcaacttcct gattcgtttc gtcatggcta aagtcttcca agggaaaaaa
    19261 caagaagaga tcgttgcgac aatagaatcg aaaccaacga atcaaaacca tgaagggggc
    19321 gaactataat gtcgactatc gaattaattg ccttgatcct tacttctaca ctggtttatt
    19381 caacaccgct aattctaacg tcactaggcg gaactttttc tgaacgaagc gggatcgtaa
    19441 acgtagggct agagggaatc atggtcatgg gtgcttttag ttccatcgtt ttcaacttga
    19501 cgatgagtcc atcacttggt tctatgacac catggatcgc tattttagtg ggtggcgtcg
    19561 taggtatcct attttctctg attcacgcag tagcaacagt cagtttgcgt gctgaccata
    19621 ttatcagcgg gacagtaatt aatctgatgg cacctgcttt aggtgttttc ttgatcaaag
    19681 catggtatgg taaaggtcaa actgataaca tcacgcagaa tctggggtat ttttctttcc
    19741 caggattagc agatattccg gtcattggac caattttctt taaaaataca ttcttacctg
    19801 cttattgtgc tattttgatt gctgtcctag cttgggtgat catcttcaag acgaaattcg
    19861 gcttgcgcct ccgttcagtt ggtgaaaatc ctcaagctgc cgacacatta ggtatcaatg
    19921 tatatgctat gcgttattca ggcgtactga tttctggctt gttaggcggt atgggcggtg
    19981 ctgtttttgc tcagtcgatt tccggtaact tttcagctgg tacgatcgtt ggacaagggt
    20041 tcatctccat ggctgctatg atctttggta agtggaatcc tttaggggca atgggtgcct
    20101 cattgttctt tggttttgct caaagcttga gtatcattgg cgcacaatta ccagttattt
    20161 cttcgattcc gccagtattg ctgcaaattg ctccatattt gctgacaatc attgtgctgg
    20221 ttgttttctt agggaaagct tctggtccaa aagcaaacgg gaagaactat atcaaatcaa
    20281 aataatcttt tagcgaataa aaagggtggg ttacgggaag tgtttttcgt ctcgcccttt
    20341 ttgttttaaa ggcaataaaa ataaaaagaa agaaggaata atcatgttta aacgcgtaca
    20401 tctgatcgtc atggattcag taggaattgg agaagcacca gatgccgata agttcggtga
    20461 tgttggttct gatacattag gacacattgc gaaagaagca ggattgaacg ttccacattt
    20521 agagcagctt ggtttaggaa cgatccgtcc tcttgaaggt gtggaatcag tgaaagatca
    20581 tcaaggctat gcgacgaaat tagaagaagt ttctgtaggg aaagatacga tgacaggcca
    20641 ctgggaaatc atgggattga atatcaaaac tccatttcgt gtgtttccta atggattccc
    20701 tgatgatctg atcaaacaaa tcgaagaatt ttctggccgt aaagtcgtat gtaacaaacc
    20761 ttatagcgga acagctgtta ttgacgatta cggtgaacac caaatgaaaa caggcgattt
    20821 aattgtatat acttctgccg atcctgtcct tcagatcgcg gcacatgaag aaatcattcc
    20881 tttagaagaa ttatatcgga tttgccaatt cgttcgtgat ataacaaaag atgatccgta
    20941 tatgattggc cgaatcattg cacgtccgta cgtgggtgaa ccaggagact tcacacgaac
    21001 aagcaatcgc catgattatg cgttgaatcc attcggacac acagtccttg attctttgaa
    21061 agaagcagga aaggacgtga ttgctgttgg aaagatcaac gatattttca acggacaagg
    21121 aatcactgaa tctgtccgca cgaaaagcaa tatggatggc gttgaccagc tgctgaaagt
    21181 tatgaaaaac gatttttcag gaatcagctt taccaattta gtagattttg atgcattata
    21241 cggtcatcgt cgggatgtag tcggctatgc gcatgcgatt gaagaatttg acttacgaat
    21301 tccagaattg cttgaagcga tggaagaaga cgacttgcta atgattacag ccgatcacgg
    21361 aaacgatccg acgtttactg gtaccgatca tacgcgagaa tatgtaccgt tattggttta
    21421 cagtaaaaaa atgaaagaac aaggggcatt gcctcaagga tattattcag atatttcagc
    21481 aacgattgct gagaacttta gtgtttcttc gactgaaaat ggcgaaagct tcttgaaatt
    21541 attaaaataa tctttttaaa taggagaaag caaatgacca agttaagcga gatgttacaa
    21601 gaaacgaccc agtttatcct tgataaagga gtaaaagaaa ttgattttgg tttgattcta
    21661 ggttcaggat taggtgaatt agcagatgaa atcgaagatg ctgtaatgat ttcttatgat
    21721 gagatcccgt tctttcctgt ttcgacagta gttgggcatg caggaaagct agtttatggg
    21781 tctctggcag gcaagaaagt gttagccatg caagggagat tccactacta tgaaggtcat
    21841 tccatgcaaa cagtgaccta tccagtacgt gtgatggccg gactaggggc ccattcagtc
    21901 attgtgacta atgcatgtgg tggtgtcaac gaatcatttg taccaggaga tttgatgctg
    21961 attaccgatc atatcaattt tactggtgaa aatcctttga tcggtccgaa tgaagatgca
    22021 atgggcccta gatttccaga tatgagtcaa gcttatacgc ctgactatca agcaacagca
    22081 aaagcagcag cagaaaaact aggtttggat cttaaagaag gcgtatatat gggctattct
    22141 ggaccaactt atgaaacgcc tgcggagatc cgaatgtctc ggacaatagg agccgatgca
    22201 gtagggatgt cgactgttcc agaagtcatc gtagcagctc acagcggatt aaaagtgtta
    22261 ggaatctctt gtattaccaa cttagcagca ggtatgcaag cgaatctgag ccatgaagaa
    22321 gttgtagaaa caactcagag agtcaaacaa tcttttaaag cattggttaa agaaacattg
    22381 acattattgt aataggagga acaataaaat gagtgtgcat attgaagcga aagaaggcga
    22441 gatcgccgat aagattttac ttccgggaga tcctttgcga gctaaatata ttgcggagac
    22501 ttttttggaa gatccagttt gttacaatca ggtacgcggt atgttaggct ttaccggcaa
    22561 atataaagga gaacgtattt ctgttcaagg aacaggaatg ggaatgccat cagccaccat
    22621 ctatgcccat gaattgatcc agtcgtatgg tgtaaagaaa ttgattcgag taggtacatg
    22681 cggtgcattg tcaaaagacg tccatgttcg tgatttagta ctagcacaag gtgcagcgac
    22741 cagttcttcg atgatcgaaa aaaatttcca agcctttcat tttcctccaa tcagtgattt
    22801 caatctatta ctgaaagcat atgagatagc taaagaaaag ggctatacgg ttcatgtagg
    22861 aaatgttctt tcggaagatt ctttctacaa agatgatttg acagagacgt tccaattagc
    22921 tgaacttggc gttttaggag tggaaatgga agcagcggct ctttattact taggcgcgaa
    22981 ataccatgtg cagacattaa gtttgatgac cgttagcgac catttgatca ccggtgaaga
    23041 aacaacagct gcagaacgtc aatctacgtt caatgaaatg atcgaagtag gattggaaac
    23101 agcgatagca gaataagaag gaaatgaaaa agctgccctg cattaagtaa taagaacatc
    23161 caagcaaaaa tagctcctca tgtttttgac ttggctggac ttattattgc aaatgtaagc
    23221 ttttgaacac cgtttaccaa gaggttgtga atctgccgtt taactctgag tcacaaggaa
    23281 caatttcaaa aaacagcttt tcatgtttga tgaatattgt gacttgtgac cgagaagttg
    23341 gcaggagaaa accgtttata cgaaaaaagg gactgtgaca aatcgtgtca cagtcccttt
    23401 tttatagtgt tttttatagg tagtattttt tagcaactgt taagtcatca tttaattcat
    23461 aaacaagtgg ttgacctgtt ggaatttcaa gatccatgat gtcttcgtca gagatacctt
    23521 cgatatgttt agctagggcg cgtaatgagt taccatgtgc agctactaaa acagttttgt
    23581 tgtctagtaa tgctggagca atttcatctt gccagaatgg caaagcgcgt tctaatgtca
    23641 ctttcaagtt ttctccacca ggaatatcgc gtttgtctaa cattgcgtaa cgacggtcat
    23701 ttgctgcaga accttcgtca gtttcttcca ttaatggagg aagagtatcg taagaacgac
    23761 gccagatgtg cacttgttcg tcgccgtatt tttcagctgt ttcttttttg ttcaaacctt
    23821 gtaatttacc atagtgacgt tcgtttaagc gccatgattt gatttgtggt acccaaagtt
    23881 gatctgaata ttctaagatc aagttacatg ttttgattgc acgagttaaa acagaagtgt
    23941 aagcaacatc aaattcgatt ccagcttctt tgattttgcg tccgccttct tttgcttctt
    24001 cgattccttc tggtgctaga tttacatcag cccaaccagt aaattggttc aatgcattcc
    24061 attcactaag accgtgacga gaaaaaacta attttggcat gtttagctct ccttttaaga
    24121 taggtttata tttctgaaat tttcagttct gttttattgt acaatttttt ggaagagatt
    24181 tccatcgaaa aattagataa gattctatga gaaaacaagt agagaatata aaaaaacgaa
    24241 cagtgcccac gcaactgttc gtttttaggt attagatgca taattgctcg atttcttttt
    24301 caggtaaatt gatatattta taacatgttc caactgaaac accgcatttt gaagaaataa
    24361 attggatcgt ttcttttttt tcataataga gacgtcggat ttttttgatc gtttttgcat
    24421 cgatttttgg tcttccgccg atttttcctt ttttacgagc ttcatctaat ccaactaatg
    24481 tacgttcttt gatcaatgcg cactccatgt ttgctaggtt ttccattaat tggaaataga
    24541 tttttcccat ttcgcgcgta tcgattcctt catcaagact aatcaagtgc agcccgcgat
    24601 ctttgaataa ttgcgtgagt tctgttaatt gccgcgtaga tttgcctaat cgatgaagtt
    24661 ggcaaatgac tagtgcatcg ccttgttgca tgtcagcgat cactgactcc aaatgaacag
    24721 cagtagaatc attttgttcc acttcatatg tctcatggaa aatttcttga caaccaaagt
    24781 cagataattg atcaacctga gttgttaaat caacgtcatt tatggttgtg cgtgcataac
    24841 caataatttt catggtgatt cctttctagc agtgattttc taactggata taacagtaaa
    24901 aatgtagcat aaagtacgtc aaaaagatag ttttttcgca aattttccgc tgtctttttt
    24961 tattggatga tcaagcagtt tttctttatt tacgacggat tgcgtcaaga atctcaacgc
    25021 ctttttctga gccgacaact actttagaaa ccatgtttag gaaaagccca tgttctacta
    25081 ccccaacaag ttcatctaaa taggtagcta agctttctgg ctgttcgatg acttctagat
    25141 gcagatcaat gatgtaatga ccaccatctg tggttaagac ttctccgttt tcgtctgtac
    25201 gcaagacggg ttggaatctt ttttcttcaa aaatatgaag tagctgttgg gagccataag
    25261 ggatcacttc aactgggagt gggaacttcc caagtttatc gactaattta gaagaatcaa
    25321 cgatccaaat cgtttctttt gagtacgtag cgactatttt ctcgaaaagc aaagcagcgc
    25381 cgccgccttt gatcccttga aaatcagcac tgatttcatc agcaccgtct attgtcaaat
    25441 cgactacggg gacttcatca atactcttta acggtattcc taatttttcc gcttgttctt
    25501 ttgtcgcatt tgaagtagta actcctgtga tacgtaaacc ttcttcacgc atacggcgac
    25561 caatctcttc taccatataa taggcggttg aaccggtccc gagacctacg atcatcccgt
    25621 cctttacgaa tttcgcggct tcaatgccag ctaattgttt taaattcatc tttgcgtcca
    25681 ccttttttta gtttgagcat cgtgctttcc tcactatttt agcgtgattt acggtaaaaa
    25741 gataccattc ttttttattc caataaagct ttttcaaaaa aaattgcatc tcttgttgac
    25801 atgttttttc attcgtgata ttctaacaaa ggtgctttat ctacaggttt ctgtgaagaa
    25861 acgtgctgac tgtttatcaa agcatatttg ataaggatgc agacattgca tttatttttt
    25921 atcacaaaaa aagaaccacc tggatgtgtg gaactagacg caaatcgtgg gaaggaggaa
    25981 acaaggaatg cctacaatta accaattagt acgtaaacct cgtaaatcaa aggtggaaaa
    26041 atctaattca cctgctttga acaaaggata taacagcttt aagaaaactc aaactaacgt
    26101 aaactcacct caaaaacgtg gggtagcaac tcgtgtgggt acaatgacac ctaaaaaacc
    26161 gaactcagct ttacgtaaat atgcacgtgt tcgtttgtca aacttaattg aagttactgc
    26221 ttatatccca gggattggtc ataacttaca agaacacagt gttgtattat tacgcggtgg
    26281 tcgtgtaaaa gacttaccag gggtacgtta ccatatcgtt cgcggtgctt tagatactgc
    26341 tggtgttaac gaccgtaaac aaagccgttc taaatacgga actaaaaaac ctaaagctta
    26401 attttaatta ttaaacaaat gaaacttgct tctactatat agaagtaatt actatgaaca
    26461 atatttcgga aggaggagtt acggatgcca cgtaaaggtc ctgttgcaaa acgtgatgtt
    26521 ttaccagatc ctatttataa ctcaaaatta gtaactcgct tgatcaaccg tgtaatggtt
    26581 gacggaaaac gcgggattgc agctaacatt atctataatg cctttgactt gatcaaagaa
    26641 tctacaggta acgatccatt agaagtgttt gaacaagcaa tgaaaaacgt tcaacctgtt
    26701 cttgaagtta aagcacgtcg tgttgggggt tcaaactacc aagtaccagt tgaagttcgc
    26761 cctgaacgcc gcgttactct aggtcttcgt tgggtagtaa actacgctcg cttgcgcggt
    26821 gaacacacaa tggaagaacg cttagcaaaa gaaatcatgg atgctgcaaa caacacaggt
    26881 gcttctgtta aaaaacgtga agacactcat aaaatggctg atgccaaccg tgcgtttgct
    26941 cactatcgct ggtaagattc tctctgtttg gtcggctttt ataagtcgac ctcagatagt
    27001 gtccttattt ttagcaacta gatagtaaag agaggagaaa caaggagaat gaccagagaa
    27061 ttttctttac aaaacactcg taatatcggt atcatggccc acatcgatgc tggtaaaacg
    27121 actgctactg aacgtatctt gtactatact ggtaaaatcc ataaaattgg tgaaactcat
    27181 gaaggagctt cacaaatgga ctggatggaa caagaacaag aacgtggtat tacgatcact
    27241 tctgctgcta ctacagcaca atggaagggt caccgaatca acatcatcga cacaccagga
    27301 cacgtagact tcacagttga agttgaacgt tcattgcgtg tacttgatgg tgctgtaact
    27361 gttcttgatg cacaatcagg tgttgaacct caaactgaaa cagtttggcg tcaagcaaca
    27421 acttatggtg tacctcgtat cgtatttgca aacaaaatgg acaagatcgg tgctgacttc
    27481 ttatattcag tatcaacttt acatgaccgt ttgcaagcaa acgctcaccc aatccaatta
    27541 ccaattggtg cggaagacga cttcacagga attatcgacc ttgtaacaat gaaggctgaa
    27601 atgtatacaa acgatcttgg aacagagatc gaagaaacag aaattcctga agaataccgc
    27661 gaattagctg aagaatggcg cgaaaaattg gttgaagctg tagctgaaac tgatgaagaa
    27721 cttactttga aatacttaga aggtgaagaa atcactgaag ctgagttaaa agaaggtatc
    27781 cgccgcgcaa cagttaacgt agaattctac ccagtactat gtggttcagc atttaagaac
    27841 aaaggggttc aattactatt ggacgctgtt cttgattact tgccatcacc gttagatatc
    27901 ccagctatca aagggattga ccctaaaact gatgaagaag tagaacgtcc tgcagacgac
    27961 agcgctcctt tctcagcttt agcatttaaa gttatgactg acccattcgt aggtcgttta
    28021 actttcttcc gtgtgtactc tggtgtttta caatcaggtt catacgtaca aaacgcaaca
    28081 aaaggcaaac gtgaacgtgt tggtcgtatc ctacagatgc atgccaactc tcgttcagaa
    28141 atctctgaag tttacgctgg agatatcgct gctgctgttg gtttgaaaga cacaactact
    28201 ggggacacac tatgtgatga aaagagtcta gttattcttg aatccatgga attcccagaa
    28261 ccagttatcc aagtcgctat tgaacctaaa tcaaaagctg accaagataa aatgggtgtg
    28321 gctttacaaa aactatctga agaagatcct actttccgtg ctgaaacaaa cgtggaaaca
    28381 ggcgaaacaa tcatcgctgg tatgggtgaa cttcacttag atatcatcgt tgaccgtatg
    28441 cgtcgtgaat tcaaagttga agctaacgta ggtgcgcctc aagtatctta ccgtgaaact
    28501 ttccgtgctg gcactcaagc cgaaggtaag tttgtacgtc agtctggtgg taaaggtcaa
    28561 tacggtcacg tatggattga atttacacca aatgaagaag gtgctggttt cgaatttgaa
    28621 aatgcgatcg tcggtggggt tgttcctcgt gaatacattc cagctgttga aactggtttg
    28681 aaagatgcta tggaaaatgg tgtcttagct ggctatccat tagtagatat caaagctaaa
    28741 ctttacgatg gttcatacca tgatgtcgat tcaaatgaaa ctgccttccg tgttgctgct
    28801 tctatggcat tacgtgcagc agctaagaaa gctaatccag taatccttga accaatcatg
    28861 gctgtagaag ttgttatccc tgaagattat ttaggtgatg ttatgggtca cgtaactgct
    28921 cgtcgtggac gtgttgaagg tatggaagct cgcgctggcg gccaacaagt tgttcgtgcg
    28981 atggttccac ttgctgaaat gtttggttat gctacaactc ttcgttctgc tactcaaggt
    29041 cgtggtacat ttacaatgac atttgaccac tacgaagatg taccaaaatc agtacaagaa
    29101 gaaatcatta agaaaaacgg cggaaaagct gaataatttc ttgaaaccgg atagtcctgc
    29161 aaagagatta tccaaagaga ctatattttt aatatagttt cgtgtaaaat aattatcgat
    29221 gatatgggca tgtggtgtac cttgtgtaca tcatatacct atcagacaaa taaaaaatat
    29281 attgtatttt taggaggaca ttttaaaatg gcaaaagaaa aatttgaccg ttctaaacca
    29341 cacgttaaca ttggtactat cggacacgtt gaccatggta aaactacatt aacagctgca
    29401 atcacaactg tactatctaa gaaaaacggc ggccaagcta tggcttacga tcaaatcgat
    29461 ggtgctccag aagaacgcga acgcggaatc acaatctcaa cagctcacgt tgagtatgaa
    29521 acagatacac gccactacgc tcacgttgac tgcccaggac acgcggatta cgttaaaaac
    29581 atgatcactg gtgctgctca aatggacgga gctatcttgg tagtttctgc tgctgacggc
    29641 ccaatgcctc aaactcgtga acacatccta ttgtctcgtc aagttggtgt tccttacatc
    29701 gttgtattct tgaacaaagt agacatggtt gatgacgaag aattactaga attagttgaa
    29761 atggaagttc gtgacctatt aacagaatac gaattccctg gtgacgatgt tcctgtagtt
    29821 gctggatcag ctttgaaagc tctagaaggc gacgcttcat acgaagaaaa aattcttgaa
    29881 ttaatggctg cagttgacga atacatccca actccagaac gtgacaacga caaaccattc
    29941 atgatgccag ttgaagacgt gttctcaatt actggacgtg gtactgttgc tacaggtcgt
    30001 gttgaacgtg gacaagttcg cgttggtgac gaagttgaag ttgttggtat tgctgaagaa
    30061 acttcaaaaa caacagttac tggtgttgaa atgttccgta aattgttaga ctacgctgaa
    30121 gctggagaca acattggtgc tttactacgt ggtgttgcac gtgaagacat ccaacgtgga
    30181 caagttttag ctaaaccagg tacaatcaca cctcatacaa aattctctgc agaagtatac
    30241 gtgttgacaa aagaagaagg tggacgtcat actccattct tcactaacta ccgtccacaa
    30301 ttctacttcc gtacaactga cgtaacaggt gttgttgaat taccagaagg aactgaaatg
    30361 gttatgcctg gcgacaacgt aacaatggaa gttgaattaa tccacccaat cgctatcgaa
    30421 aacggtacta aattctcaat ccgtgaaggc ggacgtacag ttggtgccgg tgtcgttaca
    30481 gaaatcaaag cttaattcac ttaagtttta aagtaaagca acatttattc ggacgtaatc
    30541 gctcggaacg aaggtcttag gatctttgtt ccgagctttt ttatttattg caggatcagg
    30601 aaaaaagaca ggagtattat aaatagaaga aacacgaaat aaaagagtca gtattttcta
    30661 aatgaaactt tatgaaaaca aaatgttttt tacaagaaaa agccttgctt tgtgagttta
    30721 tatatattat aatgacaaag gtgcgtttac tttagtgaat tgcacagacg taatcagaat
    30781 gatttcgtcg gctaagaaac gagaggttgc gacacgcccg gacgctttgc cacgaacgag
    30841 cgtgacggaa attttcgtgg agctatgtct acttttcaaa ataggcgaag gagggaacaa
    30901 gatggcaaaa caaaaaattc gtatccgttt aaaagcgtat gaacaccgta ttttagatca
    30961 atcagcggat aaaatcgtgg aaactgcaaa aagaactgga gctagcgtgt ctggtccaat
    31021 tccattacca actgaacgca gtctttacac aatcatccgt gcgactcata aatacaaaga
    31081 ttcacgcgaa caattcgaaa tgcgtacaca caaacgtctt atcgacattg tgaacccaac
    31141 accaaaaact gttgatgctt tgatgaagct agacttacca tctggtgtaa acatcgaaat
    31201 caaattataa aactaaaaca aaaataatgg aggtgtactc atgaccaaag gaatcttagg
    31261 gaaaaaagtg ggaatgacac aaatcttcac tgaatctggt gaattaattc cagtaactgt
    31321 tgtagaagct actccaaacg tagtactaca agtgaaaaca atggaaacgg atggctacga
    31381 agctattcaa gttggttacc aagatatgcg tgaagtttta tcaaacaaac ctgcgaaagg
    31441 tcatgttgca aaagcaaaca cggctcctaa gcgcttcatt cgtgaattca aaaatgttga
    31501 gctaggagaa tatgaagtag gaaaagaaat tacagtagat gttttccaag caggagacat
    31561 tattgatgta acaggtacta cgaaaggtaa aggattccaa ggggttatca aacgccacgg
    31621 acaaagccgc ggaccaatgg ctcacggttc tcgttaccat cgtcgtcctg ggtcaatggg
    31681 tccagttgca cctaaccgtg tatttaaaaa caaacgtctt gctggccgta tgggtggcaa
    31741 ccgcgtaact atccaaaact tggaagttgt acgtgtcgat gcagaaagaa atgttatctt
    31801 gatcaaaggt aatgttcctg gagctaaaaa atctttaatt actatcaaat cagctgtgaa
    31861 agctaaataa ttgtaggaag aaaggaggaa ctaaggaatg ccgaatgtag cattattcaa
    31921 acaagatgga agccaaaacg gtgaaatcac acttaacgaa gaaatcttcg gaattgaacc
    31981 taatgaaagt gttgtttacg atgcaatcgt aatgcaacgt gcgtcattaa gacaaggaac
    32041 acacgcggtt aaaaaccgta gcgctgttcg cggcggcgga cgtaaaccat ggcgtcaaaa
    32101 aggaacaggt cgtgcacgtc aaggatctat ccgctcacca caatggcgtg gaggtggcgt
    32161 agtctttgga ccaacaccac gttcttacag ctacaaactt cctaaaaaag ttcgtcgttt
    32221 agcaatgaaa tctgtattgt cagaaaaagt tgctgaaaac aacttggtag caatcgaagg
    32281 cttgaacttc gacgcaccaa aaacaaaaga attcaaacaa gttcttgcta acttatctat
    32341 cgactctaaa gtattggttg ttctagaaaa aggtaacgac tttgctgctt tatcagcacg
    32401 taacttacca aacgtttctg tagtaacttc tgataatgtg agtgtgctag acgttgtatc
    32461 aaatacaaaa gtgttagcaa cacaaactgc tcttactcaa attgaggagg tgcttgcata
    32521 atgaacttac tagacgtaat caaacgccca gtgatcactg aaaaatcaat gcttgccatg
    32581 gatgacaaga aatacacttt tgaagtggac actcgcgcaa acaaaacttt agtaaaacaa
    32641 gcggtagaag ctgcttttga cgttaaagtc aaaaacgtga acatcgtgaa cgtacgtgcg
    32701 aaattcaaac gtatgggcaa atatgcagga tatacaaaaa aacgtcgcaa agcgatcgtt
    32761 acattgactg aagattcaaa agaaatccaa ttattcgaag ctgctgaata agcactaata
    32821 gataactcat taaattagga gggaaaaacg tggcgattaa aaagtacaaa cctacctcaa
    32881 atggtcgtcg taacatgaca ggttctgatt ttgctgaaat cacaacatca acacctgaaa
    32941 aatcattgtt acaaccattg aaaaaccatg ctggacgtaa caacaacggc cgcatcactg
    33001 tacgtcatca aggtggcgga cacaaacgcc aatatcgcgt gatcgacttc aaacgtaaca
    33061 aagataacgt tgttgcaact gttcaaacga tcgaatatga tccaaaccgt tcagctaaca
    33121 tcgcgttagt acactatgaa gatggagtga aagcttacat cttagcacct aaaggactag
    33181 aagtgggtat gaaacttgtt tcaggtccag aagcagatat caaagtcgga aatgctcttc
    33241 cattagaaaa cattccagtt ggtactgtta tccacaacat tgaaatgaaa cctggtaaag
    33301 gtggacaatt gatccgttct gctggtacaa gtgctcaagt acttggtaaa gaaggcaaat
    33361 acgtattgat ccgcttgaac tctggtgaag ttcgtatgat cttagctgca tgccgtgcta
    33421 caatcggttc tgtaggtaac gaacaacacg aattaatcaa catcggtaaa gctggacgct
    33481 ctcgttggat gcgtaaacgc ccaactgtac gtggtagcgt aatgaaccct aatgatcacc
    33541 cacacggtgg tggtgaaggt aaagctccta tcggacgtaa agcaccagtt tctccttggg
    33601 gtcaaccagc tcttggttac aaaactcgta acaaaaaagc taaatcagac aaacttattg
    33661 ttcgtcgtcg caaaaataaa taattacttt gaccatgtgt cgcacatttt gagaggaggt
    33721 tcaccatggg tcgtagttta aagaaaggac cttttgtcga tgagcattta atgaaaaaag
    33781 ttgaagctca agcaggggtt gaaaagaaaa aagttatcaa aacttggtca cgccgttcaa
    33841 cagttttccc aagttttgtt ggatatacca tcgcagttta tgatggacgt aaacatgtac
    33901 cagtttacat ccaagaagac atggtaggac acaaattagg tgaatttgca cctacaagaa
    33961 cttatcgtgg acatgctgct gacgataaga aaacgaaacg ctaatttgag gggaggataa
    34021 atccaaatgg cagaacaaat cacatcagct aaagcaactg caaaaacagt tcgcacttca
    34081 cctcgaaaag cccgtttagt aattgacctt atcagaggta aaagtgtcgc agatgcaatt
    34141 tcaatcttga aattcacacc aaacaaatct gctggaatca ttgaaaaagt attgatgtca
    34201 gcagttgcga atgcagaaaa taactttgac ttagatgttg aaaacttggt agtaagcgaa
    34261 gcttttgtta acgaaggacc tacaatgaaa cgtttccgtc ctcgtgcaaa aggatcagct
    34321 tctccaatca acaaacgtac aagtcatatc acggtagtcg tatcagaaaa ataaggaggg
    34381 ataatcagtg ggtcaaaaag tacatccaat tggaatgcgt gtaggcatca tccgcgactg
    34441 ggatgctaaa tggtatgctg aaaaagagta tgctgagttc ttacacgaag atttaagaat
    34501 ccgtaaattt atttcaacta aacttgctga tgctgctgta tcaacagttg aaatcgaacg
    34561 cgcaacaggt cgcgtaaaca tttcaattca cacagctaaa ccaggtatgg ttattggtaa
    34621 aggcggttct gaagtcgaaa gcctaagaaa agaattaaac aaattaactg gtaaaagaat
    34681 ccatatcaat atcgtggaaa tcaaaaaacc agatctagat gcaaaattag taggtgaagg
    34741 aatcgcacgt caattagaaa accgtgttgc tttccgtcgc gctcaaaaac aagccatcca
    34801 acgctcaatg cgtgctggcg ctcaagggat caaaactcaa gtatctggtc gtctaaacgg
    34861 agcagatatc gctcgttcag aaggatactc agaaggaact gttcctcttc atactttacg
    34921 tgcggacatt gattacgcat gggaagaagc agatacaaca tacggaaaac taggagttaa
    34981 agtgtggatc tatcgtggag aaattcttcc aactaaaaaa aacactgaga aaggagggaa
    35041 ataatcatgt tagtacctaa acgtgtaaaa caccgtcgtg aattccgcgg aaaaatgcgc
    35101 ggggaagcta aaggcggaaa agaagtagca ttcggtgaat acggtttgca agctgttgat
    35161 tcacattgga tcacaaaccg ccaaatcgaa gctgctcgta tcgcaatgac tcgttacatg
    35221 aaacgtggtg ggaaagtatg gattaaaatt ttccctcaca aatcttatac tgccaaagca
    35281 attggggtac gtatgggttc tggtaaaggg gcacctgaag gatgggttgc accagtaaaa
    35341 cgtggtaaaa tcatgtttga aatcgcaggc gttcctgaag aagtagctcg tgaagcgtta
    35401 cgtctagctt ctcacaaatt accaatgaaa actaagatcg taaaacgtga ggaaatgggt
    35461 ggtgaatcga atgaaggtta aagaaatcag agaattaacc actgccgaaa tgcttgatca
    35521 agaaaaacaa ttaaaagaag aattgtttaa tcttagattc caattagcaa caggtcaatt
    35581 agaaaacact gcacgtatta aagaagtacg taaatcgatt gcacgcatca aaactgtgtt
    35641 gcgtgaacaa gctaagtaat tatggaagga ggccattaag cgtatgactg aaggaagaaa
    35701 tcaacgcaaa gtttatcaag gtcgcgtggt ttcagacaaa atggataaaa caattaccgt
    35761 tgctgtcgaa acaaagaaaa accaccctat ctatggcaaa cgcatgaact attctaagaa
    35821 atacaaagtg catgatgaaa acaacacagc aaaagttggc gacatcgtga gaattatgga
    35881 aactcgtcca ttatcagcta caaaacgttt ccgtttgttg gaagtagtcg aagaagcagt
    35941 tattatctaa taacacgaga ttttcctata tagattgaat gcaaaatctg aaaggaggat
    36001 acacaacgtg atccaagcag aaagtcgttt aaaagtcgct gataactcag gcgcacgtga
    36061 aattttgacg atcaaagtcc ttggtggatc tggtcgtaaa actgctaaca tcggtgatgt
    36121 gatcgttgct acggttaaac aagcaacgcc aggtggggtt gtcaaaaaag gtgacgtcgt
    36181 taaagccgtt atcgttcgaa caaaatcagg agctcgtcgt acagacggtt catatattaa
    36241 atttgatgaa aatgctgcag tgattattcg tgatgataaa agccctcgtg gaacacgtat
    36301 ctttgggcca gttgcacgtg aactacgtga aaacaacttc atgaagatcg tttcactagc
    36361 accagaagtt ttataatctt gagacactta tcaaaggagg tgcgaaacag ttatgtttgt
    36421 taaaaaaggc gataaagtta aagttatcac tggaaaagac aaaaacaaag aaggcgttgt
    36481 actagcagcg ttcccgaaaa aagataaagt tattgtcgaa ggtgtaaatg tcgttaaaaa
    36541 acaccaaaaa ccttctcaag ctgctcctca aggtggaatt gttgagatgg aagcgccaat
    36601 tcatgtttct aacgtgatgg tggttgactc tactggtgta gctggccgag ttggctacaa
    36661 agaagtagac ggcaaaaaag tccgtgtttc taaaaaaacc ggtgaagttt tagataaata
    36721 aaagtttaag gaaggagggt cttactgaat gaaccgccta aaagaaaaat atcttaaaga
    36781 agtaactcca tcattgatgg aaaaatttaa ctatagctct gttatgcaaa cacctaaagt
    36841 tgaaaaaatc gtcatcaaca tgggtgttgg tgatgcggta tcaaacgcta aaaacttaga
    36901 taaagcagtt gaagaattaa ctttgatcac tggtcaaaaa ccaatgatta caaaagctaa
    36961 aaaatcaatc gctggattcc gtttacgtga aggaatgcca attggtgcaa aagttacatt
    37021 gcgcggagaa agaatgtacg aattcttaga caaattggtt tctgtttcac taccacgtgt
    37081 acgtgacttc catggtgtaa gtaaaaaagc ctttgacggt cgtggaaact atactttagg
    37141 tattaaagaa caattgatct tcccagaagt agattatgat ttagtagata aagtacgtgg
    37201 tatggacatc gttattgtaa caacagcaaa ctctgatgaa gaatctcgtg agttgttgac
    37261 acaattaggc atgccattcc aaaaataatt aaaggaggcg aactacgtgg ctaaaaaatc
    37321 aatgattgct aaaaacaaac gtcctgcaaa attttcaaca caagcttata ctcgttgcga
    37381 acgttgcgga cgtccacatt cagtttatcg taagttccat ctttgccgta tttgcttccg
    37441 cgaacttgcc tataaaggtc aaattcccgg cgtgaagaaa gctagctggt aaaaagtaaa
    37501 ttcgcataag aaggaggtaa aagatcaatg gtcatgacag atccaattgc agattttcta
    37561 actcgtatcc gtaacgcaaa catggttaaa catgagtctt tagaagtacc tgcatcaaaa
    37621 attaaacgtg acatcgctga aatcttgaaa cgtgaaggtt tcgttcgtga tgttgaatac
    37681 atcgaagatg acaaacaagg cgtgatccgt gttttcctaa aatttggtaa aaacggcgaa
    37741 cgtgttatca caaacttaaa acgtatctct aaaccaggtt tgcgtgctta tgtcaaagct
    37801 gacgaagtac caaaagtatt gaacggttta ggtatcgcaa tcatttcgac ttctgaaggt
    37861 gttgtcactg ataaagaagc tcgtgaaaaa aatatcggcg gcgaagttat cgcttacgta
    37921 tggtaatcaa gtagaatcta caaggaggtg tctttaagtg agccgtatcg gaaataaagt
    37981 cgttgttatc cctgaaggag taacagttac tcaagacgga aacaacgtta cagttaaagg
    38041 accaaaaggt gaattgactc gtactttttc agcagatatc aaaatgaata tcgaaggaaa
    38101 cgaagtaact ttcactcgtc caaacgacag taaagaaatg aaaacaattc atggaacaac
    38161 tcgtgcgaac ttcaacaaca tggttgtagg tgtcagcgaa ggtttccaaa aagcgttaga
    38221 actaatcggg gttgggtacc gtgcacaatt acaaggatca aaacttgtat tgaacgttgg
    38281 ttactcacat ccagtagaaa tcacaccacc agctggagta actgttgaag taccagctaa
    38341 cacacaagta attgttaaag gtgcgaacaa agaagaagtt ggcgaattag ctgcaaacat
    38401 tcgtggaact cgtcctccag aaccttataa aggtaaaggt attcgttatg ttggcgaata
    38461 tgtacgccgt aaagaaggta aaactggtaa ataatcgttt agaaaaattg catagaagcc
    38521 agcagcagga aaaaggcatt tggctgaagt ttaccttcaa aaaaatacat ttttacttgt
    38581 gccgcagctt ctatttttcg aaacgtattt actgcttgaa atagtagtac aataaaaata
    38641 aagaggtgac aattgtgatt acaaaaccag ataagaacaa aacacgtcaa aagagacatc
    38701 gtcgtgtacg taacaatatc tctggtactg ctgagcgccc acgcttgaac gtttttcgtt
    38761 ctaacaaaaa catctacgct caaattattg atgacgtagc gggtgtaacg ctagcaagtg
    38821 cctctacctt ggataaagaa atttcaggtg gaacaaaaac agaagcagca caagctgtcg
    38881 gtaaactagt tgctgaacgt gcagctgaaa aaggcattaa agtagtagtc tttgaccgtg
    38941 gtggatacct ttaccatggc cgtgtgcaag ctttagctga agctgctcgc gaaaatggac
    39001 tagaatttta gaagaaggag gaataccatt catggtttat atcgatccaa aacatttgga
    39061 attagaagac cgcgttgttg caattaaccg tgtaacaaaa gttgttaaag gtggacgtcg
    39121 tctacgtttt gctgctttag ttgttgtcgg tgacaaaaac ggacacgtag gatttggtac
    39181 aggtaaagct caagaagttc ctgaagcaat ccgtaaagca attgaagatg cgaagaaaaa
    39241 cttagttgaa gtacctatgg ttggttcaac aatcccacac gaagtaatcg gtgcttttag
    39301 tggtggtaga atcctaatga aacctgctgt agaaggttct ggggttgctg ctggtggacc
    39361 agttcgtgcc gtattagaat tagctggggt agcagatatc acttctaaat ctttaggctc
    39421 aaatacacca atcaacgttg ttcgcgcaac agttgaaggt ttgaaacaat tgaaacgtgc
    39481 agaagaagtt gcagaacttc gcggcaaatc agttgaagaa ttaatcggat aaggaggaca
    39541 acaataatgg ctgaattaaa agttacttta aaacgcagta ttatcggacg tcctcaaaac
    39601 caacgcgata cagttaaagc gttaggtctt acaaagatta acagttcagt ggttaagcct
    39661 tctaatgaag caattaaagg catgatcaat actgtttctc acctagtgga cgttgaagaa
    39721 atttaattta gaaaaatcat aactagaaca ttaaggaggt gccaaaccta tgaaacttca
    39781 tgaattaaaa cctgctgaag gttcacgcca agtacgtaac cgtgttggtc gtggtacttc
    39841 atctggtaac ggaaaaactg ccggacgcgg acaaaaagga caaaaagctc gttcaggcgg
    39901 cggtgtgcgt ttaggatttg aagggggaca aacaccattg ttccgtcgtt taccaaaacg
    39961 aggattcaca aacgtgaacc gtaaagacta tgcagtcatt aatcttgatg tattgaaccg
    40021 ctttgaagac ggagctgaag taactcctgt tgctttagtt gaagctggaa tcgtgaaaaa
    40081 cgaaaaagct ggaatcaaag tattagctaa tggggaacta aacaagaaat taactgtgaa
    40141 agcagcaaaa ttctctaaag cagctcaaga agcaattgaa gcggctggtg gatcaatcga
    40201 ggtgatctaa tgtttagact attaaaagat gcttttaaag taaaagacat tagatcgaaa
    40261 atcctattta ctgtatttgt gttgtttgta tttcgcctag gtgcacatat tactgtacca
    40321 ggcgtaaatg ctgcttcatt gcaaaacctt tcttctttgc catttttcaa catgttgaac
    40381 ttggtaagtg gtagtgcgat gcaaaacttt tccattttct caatgggggt ttcaccatac
    40441 attactgctt ctatcgtcat tcagcttctt caaatggaca tcgtgcctcg atttgttgaa
    40501 tggtcgaaac aaggggaagt tggtcgtaaa aaactgaatc aagcgactcg atatctgaca
    40561 ttagtattgg catttatcca atctgtcggt atcactgctg gattcaatca atacgctcaa
    40621 ttaggtttcg tgaataatcc taacgtagca acttacgtaa cgatcggttt gattctaaca
    40681 gccggtacga tgtttgttac ttggctagga gaacagatca cagaaaaagg tatcggaaat
    40741 ggcgtatcaa tgatcatttt tgcaggtatc atttctagat tgcctgaaag tatcagagaa
    40801 atttatgaag attactttat aaatattgat ccttcagaca tatggaaatc tgctattttt
    40861 gtggccattt tactagtagc ggtcttgttg atcgtgacat tcgtaacatt tttccaacaa
    40921 gctgaacgca agatcccgat ccaatatacg aaacgcgtag ctggcgcgcc tacaagcagc
    40981 tatctgccgt tgaaagtaaa tgcagctgga gttattccag ttatctttgc tagttcgttt
    41041 attgctacgc caaatgctgt tttacaagca ttgagcggta gattcggttc tgaaaactgg
    41101 tacaaagtga tgatggagat ctttaactat aatacagcac ctggtgctac gatttatacg
    41161 attctgatcg ttgccttcac tttcttctac gcctttgttc aagtaaaccc tgagaaatta
    41221 gcggaaaact tgcaaaagca aggaagttat ataccaagcg tacgccctgg taaggggact
    41281 gaagagtatg tctcaagatt actgatgcgt ttaagtacag ttggtgcagt cttccttggt
    41341 ttagtggcat tgcttccaat cattgctcaa atggtttggg atcttccaca atcaatcgga
    41401 ttaggcggta caagcttact gatcgttatc ggtgtagctt tagaaactgc gaaacagtta
    41461 gaaggcttaa tgctgaaacg taaatatgtt ggattcattg agtaagaaag cgtgttgagg
    41521 tttcccctca acacacgttc tcacacattt attaggaggg aaaacaatga acctcatttt
    41581 aatggggctg ccaggcgcag gtaaaggaac acaagctgaa aaaatcattg atgcttatgg
    41641 tattccgcac atctcaacag gagatatgtt ccgcgcagcg atgcaaaacg aaacagctct
    41701 tggcctggaa gcaaagtctt atatggataa aggtgcatta gttcctgatg aagtaacaaa
    41761 tggaatcgtg aaagagcgac tagctgaacc agatacagag aaaggcttct tattagatgg
    41821 ctttccacgt acgctagatc aagcaaaagc tctggacgca atgctaaaag atttgaataa
    41881 aaaaattgat gctgtcatcg atatccatgt tggtgaagaa atcttggtcg aacgtctagc
    41941 cggccgattt atctgcagca attgtggagc aacttaccat aaaattttca accctacaaa
    42001 agttgaagac acatgtgatc gttgcggtgg gcatgaattc tatcaaagag aagatgataa
    42061 acctgagacg gttaaaaatc gtctggctat caacatcaaa aatagtgaac caatcttggc
    42121 ttattataaa gaacaaggtt tgctaaacac tattgatggt gctcgtgaaa tcgatgctgt
    42181 atttgcagat gtacaaaaaa tcattgaaaa atagcacgat tgcccgtagc cgacttgcat
    42241 tatgcagtta ctcttgtcaa gttcaataaa ttatgataga atatgtcagt ccgacttcta
    42301 gagaatcctc tagatgatcc attttgagaa ttttgaggtg ggaactgcag tttccgccat
    42361 tttatcgtgt gaaaatgcga aacaagtaca aggaggtact gtgcgtggca aaagaagata
    42421 tgattgaagt cgaaggtaca gtcgtcgaaa ctttgccgaa tgcaatgttt aaagttgaac
    42481 tagaaaacgg acaccaagtt cttgctactg tttcaggtaa aattcgtatg cattacatcc
    42541 gtatcttacc tggtgacaaa gtaacggttg aattgtctcc atacgatctt aaccgtggcc
    42601 gtattactta tcgctttaaa taattgtact ccgtaaaact acaggaggta ttatcatgaa
    42661 agtaagacca tcagtaaaac caatgtgcga acattgtaaa gtaattcgtc gtaaaggacg
    42721 cgttatggtg atttgcccag caaatccaaa acataaacaa cgtcaaggat aattggaggt
    42781 gctataaata tggctcgtat tgcaggagta gatattcctc gtgataaacg tgtagtagtt
    42841 tcacttactt atatctatgg tattggtaac actactgcta aaaagatctt agctgatgct
    42901 ggtgtatctg aagatgttcg cgttcgtgaa ttaacgaatg aacaaacaga tgctatccgt
    42961 gcagaagtag ataaattaaa agttgaaggg gatcttcgtc gtgaagtgaa cttgaacatc
    43021 aaacgtttga tggaaatcgg ttcttaccgt ggaatccgtc atcgccgtgg attgcctgtt
    43081 cgtggacaaa acacgaaaaa caatgcacgt actcgtaaag gcccagctcg cgctatcgct
    43141 ggcaagaaaa aataattctt aagtgaagga ggttaaaact tcatggcagc aaaaaaagtg
    43201 aatcgtaaac gccgtgtgaa gaagaatata gaagcaggta ttgcacatat ccattctaca
    43261 ttcaataaca ctatcatcat gatcactgat actcacggaa atgcattagc gtggtcatca
    43321 gctggttcat taggtttcaa aggaagcaaa aaatcaactc cttttgccgc tcaaatggca
    43381 gcagaaactg ctacaaaagc agcaatggaa cacggattaa agactgttga agtaacagtt
    43441 aaaggacctg gttctggacg tgaagcagca attcgttcat tacaagctac aggtttagaa
    43501 gtgactgcaa ttcgtgacgt gactccagtt cctcataatg gtgcccgccc tccaaaacgc
    43561 cgtcgtgttt aatgagtgcc gctcattttg agttttatca gtaacgtcat tgaacaagac
    43621 acttttcgtt ttgaaagggg taaagataag aatgattgag ttcgaaaaac caagaatcgc
    43681 aaaaattgat gaagaaaaag attatggcaa gttcatcgtt gaacctcttg aaagagggta
    43741 tgggactact ttagggaatt ccctacgtcg tattttatta tcttctttgc caggtgctgc
    43801 catcactaat attcaaatcg atggtgtact gcatgaattc tctaccatca aaggtgttag
    43861 agaagacgtc actcaaatca tcttgaacat taaaggctta gcgctgaaaa tgtatggcca
    43921 agaagaaaaa acccttgaaa tcgatattac cggacctgct acagtaactg ctggcgatat
    43981 tatcgttgat agtgatgtag aaattcttaa caaagatatg tacatctgta gtgtctctga
    44041 aggagctacg ttccatgctc gcttaacagt gaaacctgga cgtggttatg ttcaagcaga
    44101 tgaaaataaa aaagaagata tgccaattgg cgttcttcca gtcgattcaa tctacacacc
    44161 tgttcgtcgt gtgaactatc aagttgagaa cacacgtgtt ggacaccgtg aagattttga
    44221 taaattgacg atggagattt ggacagatgg ttcaattgaa ccgttggaag caatgagttt
    44281 gtctgcgaaa atcatgactg aacatttgga tatttttgtg aacttaactg atgaagcaaa
    44341 aaatgctgaa atcatggtcg aaaaagaaga aacacaaaaa gaaaaaatgt tggaaatgac
    44401 gatcgaagag ttagacttgt ctgttcgctc ttacaattgt ttgaaacgtg caggaatcaa
    44461 tactgtacaa gaacttacaa ataaatctga gcctgaaatg atcaaagtcc gtaacttagg
    44521 acgtaaatca ttagaagaag ttaaagcaaa attgcacgac ttaggtttag gtttacgtaa
    44581 agaagattaa aaccttttac gaaggaggga aaccaacgtg agttatcgta aactaggacg
    44641 cacatctagc caacgtaaag cgatgttgcg tgatttaaca actgatttat taatcaacga
    44701 acgtattgtg actactgaag ctcgtgcgaa agaagttcgc tcaactgcag aaaaaatgat
    44761 cactttaggt aaacgcggag atttgcatgc acgtcgtcaa gctgcggctt tcgtacgtaa
    44821 tgaagttgcc agcgtgcgtg aagaaaatga ggacatcgtc attgaatcag ctttacaaaa
    44881 actgttcaac gatattgcac ctcgttacgc agaacgtcaa ggcggataca cacgcatttt
    44941 gaagacagaa ccaagacgcg gtgatggtgc accaatggtt gtcttagaac ttgtctaaaa
    45001 tcacactaat aacatcactt tatagatgat tcttttagag tgttatgatg ttggaagata
    45061 tttttccgag tctagctcag cgctgctcct caagcgaata tcgcttgggg agtaagcact
    45121 catcataaag tgttttttta taccttcaat tacctgtcat gacagatatg tttgaaggca
    45181 taaaaaaaca ttttaagcga gaaataattg aaaattttgt ctttttttga tagtgttagt
    45241 aggaacttta ggatgaagaa ggagtaatca tggagccaat tattgaatta gaaaagataa
    45301 attataaata ccagccagat gatcttcgcc ccgcattgaa agatgtctcc tttacaattg
    45361 ataaaggtga gtggatcgct attattggac acaatggttc aggaaaatcg acacttgcca
    45421 aaacgatcaa tggattgctt ctgccagaat cagggatcgt gaaagtggga aatcaaatat
    45481 tggatgaaga aaatatttgg acgatccgac agatggttgg aatggttttt caaaatccag
    45541 acaatcagtt tgttggttcg acagtagaag atgacgttgc ttttggtctt gaaaatcaag
    45601 ggattcccag agaagaaatg ctagtgcgtg tgaaagatgc acttgagaaa gttagaatgg
    45661 ctgagtttgc ttcacgcgag cctgctcgtt tatcaggagg gcaaaaacaa cgtgttgcca
    45721 ttgccggcgt ggttgcctta cgacctgata tcattatttt agatgaagca accagtatgc
    45781 tagatccaga aggtcgtgaa gaagtgatct ctacgatcaa gaagatcaaa gaagaaagtc
    45841 aattgaccgt tatatctatc actcatgata ttgatgaagc agctaatgcc aatcggatac
    45901 tagtgatgag acaaggcgaa cttgtccgtg aaggtacacc aaaagaaatt ttttctgctg
    45961 gtcctgaact gatcgactta ggtctagatc taccatttcc tgaaaaactg aagagcgcac
    46021 tgaaagaacg tggggttgat gtgcccagtg aatatatgac agaagaaagg atggtggatt
    46081 ggttatggac atccgttttg aacaagtaga cttcacttat cagccgaata caccatttga
    46141 acaaagagcc ttatttgaca tcaatatgac gatcaaagaa aacagttata ctgcattagt
    46201 agggcatacc ggaagcggga aatcaacctt acttcagcat ttgaatgcgc ttgttaagcc
    46261 aacaagcggt acagtccata ttggagaacg agatattcag ccggatacgg ataataagaa
    46321 tttaaagccc atccgaaaaa aagttggcat cgtctttcag tttccagaag cacagctatt
    46381 cgaagaaacg gtagcaaaag acattgcttt cggtcctaaa aactttggtg tcagcgaaga
    46441 agaagcacta gtcctagcaa aagaaacatt agaacaagtt gggctggatg aaagctattt
    46501 ggaacgttcg ccatttgaac tttcaggagg gcaaatgcgt agagtagcaa tcgctggtgt
    46561 gcttgccatg aggccggaag tacttgtatt ggatgaacca acggcaggac ttgatcccca
    46621 gggcagaaaa gaaatgatgg agatgttttg gcgtcttcat aaggaacagc agatcactat
    46681 tgtattagtt acgcatttaa tggatgatgt agccaatttt gcagattatg tctatgtgtt
    46741 ggaaaaggga cgaatcgtta atagcgggga gccgcaagag gtctttcaaa acattgaatg
    46801 gctaaaagaa aaacaactag gcgtacctac tgccaccgag tttgcagaag aattaatggc
    46861 taaagggatg aactttgcta ccttgccttt gacagctgaa gaattagcag acgctatcgt
    46921 ccattatgct ggaggaaatg cccatgatga ataaattgat atttggtcgt tatatgcctg
    46981 gagattcctt gatccatcga ttggatcccc gagctaaatt attagccagt ttttatttta
    47041 tcggaatcat ttttttagca aataattggg aaacgtacct tttgttggca tttttcacat
    47101 tactttctgt cttcttgtca aaagtagatt tgaaattttt tatccgaggc attcgcccat
    47161 tgatctggct aatcctcttc actgtagctt tgcaggtctt gtttacaagt ggcggagaaa
    47221 cttattggag ttggggtgtt ttccatatca ctgaatttgg tgtgatgaac ggcctcttta
    47281 ttttctgtcg attcgtgttg atcattttta tgtcgactct actcacgctt actacaccgc
    47341 ctttggaatt atcggatgcg attgagtact tgcttcgtcc attgaaagcc gtacggttcc
    47401 cagtccacga aatttcattg atgctgtcga ttgctcttcg ttttgtgcct acattgatgg
    47461 acgaaacaga aaagatcatg aacgctcagc gcgctcgcgg tgttgatttt ggtgaaggaa
    47521 acctagttca gaaaatgaaa gcagtcattc ctttgctgat tcctttattt gtcagcagct
    47581 tcaaccgtgc ggaagatctt gcgactgcta tggaagcaag aggctacaaa ggtggggaag
    47641 gaagaacgaa atatcgtgta ttgcattggc ataatagaga cacgatggtt atcattgctt
    47701 ttgtattatt aactgtgcta ctgatctttt taagaggata aacgttcagt gggccgctat
    47761 ctattctttt cttgctgatc tgtttgcaag aaaggatgca agcagtgtat gacaagaaat
    47821 attagggtct gtggcagact aaggtcacag gcctctttct tttatctgaa aagaaagaac
    47881 gaaaaa
//
LOCUS       DAZKOB010000010        45866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000010 DAZKOB010000000
VERSION     DAZKOB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.010"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..1141
                     /locus_tag="ACG8M6_000598"
     CDS             71..1141
                     /locus_tag="ACG8M6_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456290.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1156..1824
                     /locus_tag="ACG8M6_000599"
     CDS             1156..1824
                     /locus_tag="ACG8M6_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456291.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1844..2422
                     /locus_tag="ACG8M6_000600"
     CDS             1844..2422
                     /locus_tag="ACG8M6_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0456292.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2823..3734
                     /locus_tag="ACG8M6_000601"
     CDS             2823..3734
                     /locus_tag="ACG8M6_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0456293.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4112..5029)
                     /gene="rnhC"
                     /locus_tag="ACG8M6_000602"
     CDS             complement(4112..5029)
                     /gene="rnhC"
                     /locus_tag="ACG8M6_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0456294.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5231..5668
                     /gene="zapA"
                     /locus_tag="ACG8M6_000603"
     CDS             5231..5668
                     /gene="zapA"
                     /locus_tag="ACG8M6_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0456295.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5726..6274
                     /locus_tag="ACG8M6_000604"
     CDS             5726..6274
                     /locus_tag="ACG8M6_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0456296.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6389..8749
                     /locus_tag="ACG8M6_000605"
     CDS             6389..8749
                     /locus_tag="ACG8M6_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0456297.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8836..9150
                     /gene="trxA"
                     /locus_tag="ACG8M6_000606"
     CDS             8836..9150
                     /gene="trxA"
                     /locus_tag="ACG8M6_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0456298.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9332..11110
                     /gene="uvrC"
                     /locus_tag="ACG8M6_000607"
     CDS             9332..11110
                     /gene="uvrC"
                     /locus_tag="ACG8M6_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0456299.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11255..12154
                     /locus_tag="ACG8M6_000608"
     CDS             11255..12154
                     /locus_tag="ACG8M6_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456300.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12147..13409
                     /locus_tag="ACG8M6_000609"
     CDS             12147..13409
                     /locus_tag="ACG8M6_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456301.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13570..15468
                     /locus_tag="ACG8M6_000610"
     CDS             13570..15468
                     /locus_tag="ACG8M6_000610"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0456302.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15798..16517)
                     /locus_tag="ACG8M6_000611"
     CDS             complement(15798..16517)
                     /locus_tag="ACG8M6_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0456303.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    16707..16754
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16854..17186
                     /locus_tag="ACG8M6_000612"
     CDS             16854..17186
                     /locus_tag="ACG8M6_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456304.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17173..19176
                     /locus_tag="ACG8M6_000613"
     CDS             17173..19176
                     /locus_tag="ACG8M6_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0456305.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19195..19665
                     /locus_tag="ACG8M6_000614"
     CDS             19195..19665
                     /locus_tag="ACG8M6_000614"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0456306.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19686..20270
                     /locus_tag="ACG8M6_000615"
     CDS             19686..20270
                     /locus_tag="ACG8M6_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0456307.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20285..21271
                     /locus_tag="ACG8M6_000616"
     CDS             20285..21271
                     /locus_tag="ACG8M6_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0456308.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21261..21572
                     /locus_tag="ACG8M6_000617"
     CDS             21261..21572
                     /locus_tag="ACG8M6_000617"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0456309.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21594..23375
                     /locus_tag="ACG8M6_000618"
     CDS             21594..23375
                     /locus_tag="ACG8M6_000618"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0456310.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23368..24744
                     /locus_tag="ACG8M6_000619"
     CDS             23368..24744
                     /locus_tag="ACG8M6_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0456311.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24751..25386
                     /locus_tag="ACG8M6_000620"
     CDS             24751..25386
                     /locus_tag="ACG8M6_000620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0456312.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25416..26771
                     /locus_tag="ACG8M6_000621"
     CDS             25416..26771
                     /locus_tag="ACG8M6_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0456313.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26963..28357)
                     /locus_tag="ACG8M6_000622"
     CDS             complement(26963..28357)
                     /locus_tag="ACG8M6_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0456314.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28449..29420
                     /locus_tag="ACG8M6_000623"
     CDS             28449..29420
                     /locus_tag="ACG8M6_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0456315.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29417..30199
                     /locus_tag="ACG8M6_000624"
     CDS             29417..30199
                     /locus_tag="ACG8M6_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0456316.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30368..30739)
                     /locus_tag="ACG8M6_000625"
     CDS             complement(30368..30739)
                     /locus_tag="ACG8M6_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0456317.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30873..31316
                     /locus_tag="ACG8M6_000626"
     CDS             30873..31316
                     /locus_tag="ACG8M6_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0456318.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31359..32168)
                     /gene="yidA"
                     /locus_tag="ACG8M6_000627"
     CDS             complement(31359..32168)
                     /gene="yidA"
                     /locus_tag="ACG8M6_000627"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0456319.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(32187..33554)
                     /locus_tag="ACG8M6_000628"
     CDS             complement(32187..33554)
                     /locus_tag="ACG8M6_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0456320.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33538..34368)
                     /locus_tag="ACG8M6_000629"
     CDS             complement(33538..34368)
                     /locus_tag="ACG8M6_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0456321.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34531..34968
                     /locus_tag="ACG8M6_000630"
     CDS             34531..34968
                     /locus_tag="ACG8M6_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0456322.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            35044..35664
                     /gene="rpoE"
                     /locus_tag="ACG8M6_000631"
     CDS             35044..35664
                     /gene="rpoE"
                     /locus_tag="ACG8M6_000631"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0456323.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            35853..36359
                     /locus_tag="ACG8M6_000632"
     CDS             35853..36359
                     /locus_tag="ACG8M6_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0456324.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            36376..37275
                     /locus_tag="ACG8M6_000633"
     CDS             36376..37275
                     /locus_tag="ACG8M6_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0456325.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            37362..38246
                     /locus_tag="ACG8M6_000634"
     CDS             37362..38246
                     /locus_tag="ACG8M6_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0456326.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(38438..38509)
                     /locus_tag="ACG8M6_000635"
     tRNA            complement(38438..38509)
                     /locus_tag="ACG8M6_000635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38474..38476),aa:Arg,seq:ccg)
     gene            38569..39903
                     /gene="rpoN"
                     /locus_tag="ACG8M6_000636"
     CDS             38569..39903
                     /gene="rpoN"
                     /locus_tag="ACG8M6_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0456327.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            40052..41143
                     /locus_tag="ACG8M6_000637"
     CDS             40052..41143
                     /locus_tag="ACG8M6_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0456328.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            41184..42185
                     /gene="gap"
                     /locus_tag="ACG8M6_000638"
     CDS             41184..42185
                     /gene="gap"
                     /locus_tag="ACG8M6_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0456329.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            42295..43485
                     /gene="pgk"
                     /locus_tag="ACG8M6_000639"
     CDS             42295..43485
                     /gene="pgk"
                     /locus_tag="ACG8M6_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0456330.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            43589..44344
                     /gene="tpiA"
                     /locus_tag="ACG8M6_000640"
     CDS             43589..44344
                     /gene="tpiA"
                     /locus_tag="ACG8M6_000640"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0456331.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            44460..45758
                     /gene="eno"
                     /locus_tag="ACG8M6_000641"
     CDS             44460..45758
                     /gene="eno"
                     /locus_tag="ACG8M6_000641"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0456332.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        15218 a         7931 c         9782 g        12935 t
ORIGIN      
        1 aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt atttcaggag
       61 gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac gttatgcgtt
      121 agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg ggttagctta
      181 tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt cgattgtgct
      241 ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga tagcagaaga
      301 agtcgaaggt gggaaagtgg cgtatcttgc ccaaacacct ggagtcgtga ctagtaaaga
      361 cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa atcaattcat
      421 catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag taggcgatat
      481 tagtttgaaa gaagaatatg gtctagccat aggagataca gttaagttat ctggtagtga
      541 caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct caccagttct
      601 ctatacaacc atcaatgctt accaagaaat ccgcttcgaa aaagaagata caagtgaaaa
      661 tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg aagatttaga
      721 acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc aagtattgac
      781 ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg ggattttcat
      841 ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac aagggatcac
      901 aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat ttgttggtat
      961 tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg cggttccatt
     1021 ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgttagtag tagctgtgtt
     1081 aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg caattggata
     1141 agaaggagga agaacatgaa agcaatagaa tttaaagaag tagaaaaaag tttccaagat
     1201 ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa aggggaattt
     1261 gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt ggcaggcgga
     1321 ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag taatcaaaaa
     1381 gaaagcgaac gcgttaaact acgttttaaa gaaattggct ttattttgca agcttccaat
     1441 ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat taaacatgaa
     1501 catcgtcaaa aagaagcaaa cgaattattt gagcagcttg gcgtggataa gttgatcaac
     1561 aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc tcgtgcattg
     1621 tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga ttcagaaaag
     1681 gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa agcaacgatc
     1741 atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat gatggaagat
     1801 ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc atcgatacaa
     1861 caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt ccaagggata
     1921 tctcggcact tcaacaagag aaatcgcaca aaaagttggg atcacccagc ccaatctcta
     1981 tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc acttaaaaga
     2041 tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc aagtaacctt
     2101 gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc tgatgcttca
     2161 cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc tatggaagaa
     2221 aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc taagaaatgg
     2281 gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt acatcactcg
     2341 gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt tatatttgaa
     2401 tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaatagttag gtgaagaagc
     2461 aagatatgac gaagatcgtt atatcttgct tctttatata ggtagttttt cataaataca
     2521 aatttgttat tctctcatga ttatcagggt atatttttat ttattatgtc ggtgttccag
     2581 gaagtgtttt aatttaataa cgaagaaatt aattgtttgt agaatgaagg aaactattta
     2641 ttgggtagat gaatcaccac gaatagtcaa aaggagattt caggatatgc caaaaatgaa
     2701 aaaaatcatt ttagcagtaa tgttattttc tacggttata tcttttacag cccctttttt
     2761 gcttacttaa gctgatttta agttttggcc cttagtctgg atctacgagg tgagagtcaa
     2821 gaatgaaaaa aataattgga gcttttgtat ttgcaatcat tatcattatt atctgttttc
     2881 aaatagatgt ttttggtcaa aaaaagatag ttggacaaac ggtcagcagt acgaacagta
     2941 cggttacaaa gacaaaggaa tcatttgatg atacagaaca agagacatta atcaaaatcg
     3001 gaaaaaatga ccgtacacta tatacagatg actcgctaac taccccactt gcaactataa
     3061 acggcggaga gctggcggag tttctttctg aaacaaaaaa tgcttatcag atcaagacaa
     3121 atgatggcta tactggatat ttagccctcg aagatggaac caaagtcaca aagaatatac
     3181 aaacaaaacc ggaagagcta tctgatgcag tgattgtatt agatcctggg catggcggaa
     3241 atgatactgg ggcattaagc aatgatgaac aaacagaaga aaaagatata acattaagta
     3301 cagccataaa agtaaaaaag gcgctagagg atgctggagc aacggtttat ttgacccata
     3361 ctacagatga attagtacag cttggagata tatgcgacta cagtgaagaa aaaaaggcag
     3421 atgtctttat cagtttacac gcagactcta cggaatatgc gaatgaagca actggaatca
     3481 cgacatatta ttactatgga caagaggaaa cattagcaca gacaattgct gactccttca
     3541 ctgatctgcc tctggattca agaggaatct ccactgggaa ctatcaagtt cttcgagaaa
     3601 atcttcaacc ttctatttta atcgagatgg gttatatgaa taatgatagt gatttaaagg
     3661 aattagtaac agacagttat cagcagcaga ttgctgcaag tttgacacaa gcattgacca
     3721 cttattttca gtaataaata agcccagcca aacaaaaata gctcctcata tttttcgttt
     3781 ggccgggctt attatcgcag atgcaagctt ttaaacaccg tttaaggaag aggtcgtgag
     3841 cctgccgttc aactctgagt cacaaggagc aatttcaaaa aacagcttct catgtttgat
     3901 gactattgtg acttgtgacc gaggagttgg catgagaaca ccgtttaaga aaaaggttgt
     3961 gaaatcaacg ttttgttctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
     4021 tgccgtttgt tgagctaata ccgaggatca gatgattgaa caccgtttat tcggaacaag
     4081 ggactgtgac atgacttttg tcacagttcc attatttttt tagtagcttc aaagcttttt
     4141 ctgtattggc aaaatgaagt ttggcatatt tttcaagcat aggcatgccg cctttctcca
     4201 atagttttgc cgctacttgg tcagatttcg ttccagctcc tgacggaagg gtgattccag
     4261 cttcagctga tgctttgtct aactcttcca aaaagctggc acgactgatg atagaagcag
     4321 cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt tcttgtaatt
     4381 gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac tgatcgatca
     4441 aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc gcttgattgt
     4501 gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg tatttttctg
     4561 gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca agctggatga
     4621 tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat tctgcaagct
     4681 gatcacgttt cacataagca gcacatactg ttagcggacc gaaatagctg ccatttccga
     4741 cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa gaggctgttt
     4801 ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct attgctgcat
     4861 tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc ccgtcttttt
     4921 ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga tagtatgctt
     4981 tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata aaattctcct
     5041 cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa gagattgcga
     5101 gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa atatttgtgg
     5161 agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca aataggagga
     5221 aatgattcgt atggcgcacg aaaaaacacg ctacaaagcg gtgattgcca accaaacata
     5281 tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc tgatcaatga
     5341 acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag cagcaatctt
     5401 gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatat tggagctgga
     5461 agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg aaaatcgtgt
     5521 gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa atggtcaagc
     5581 agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga aacgaaaagg
     5641 acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa aaatttaaaa
     5701 aagttagaag agaaaggaac gtagcatgct gagtttgctg attttatttt tattgctaat
     5761 tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa atcatattcg cgattggata
     5821 tgtattgtca tttattgctg cccagcattt ttacaaacca ttagcaagtc atttggaatt
     5881 atacattcca tacccagctg tcaccccaac atcaaagtta gcattttttg atcaggtatt
     5941 tgcctttcac ctagatgaag ctttttatgc aggaactgct tttctgatta ttttgctaat
     6001 tggctggctg cttacccgat ttgtgggggt ttttgtccat ggattgacgt atgtgccgat
     6061 cttgagacag gtagactgga ttgcaggagg catcctgtca ctaatcatgg cttacgttac
     6121 tatatttttg attttgcagt tacttgcctt tgttccatta gatgtggtcc aaaatcaatt
     6181 cggcgagcat agtttcgctc gttttatcgt tgagagaacg ccatttctta ccaacaagat
     6241 gtatgagctt tgggtaacga atattttgaa ttaaggaaaa tagtgctgga aaatatttcg
     6301 ttttttgtac agagtagttt atttaagaag gctgcgtagc gtatgtccgg cacagctctt
     6361 ttttcatagt gaaagataag gtgataaaat gaataaccga atccttgaaa cattggaatt
     6421 tgaaaaagtc aaacagatgg tcagacaatt tgtagtgact gcacaaggaa aagaagaact
     6481 ggcagaattg gtaccagtga gtgagaaaca aacgatcaca aactggcttc aagaaactga
     6541 ggatggattg aaagtgcaac gtctgcgagg cgggatccct atacctaaaa ttgaaaatat
     6601 ccgtccccat atgaaacgga ttgagatcgg agcagacttg aatggagtgg agcttgcaca
     6661 agtttccaga gtattgtcta cgacaagcga attgaatcgt ttcattgatg atctttctga
     6721 cagtgaaatc gaatttgcac gtttgtatat gtgggcagat cagttagtca cgatccctgt
     6781 tttgagccgc cgtttaaagg aagcaatcga tgaagacgga cgcgtgactg atgacgcttc
     6841 tcctgagtta aagagcataa gacagaacat tcggcgaagt gaacaagctg ttcgtgaaca
     6901 gttagatggg atcgtccgtg ggaaaaatgc aaagtattta agtgatgcga tcatcaccat
     6961 gcgaaatgac cgctacgtta tcccagtcaa acaagaatac cgcggtgtat ttggcggtgt
     7021 agtccacgat caaagtgctt caggtcagac tttatttatt gaaccaaaac aagtggtgga
     7081 tttgaataat cgtttgcgtc aataccaaat cgctgaacga aatgagattc aacgtatttt
     7141 aagtgaactt tctgctgaac ttgttcccca tcgtcaagaa atcatccaca atacttatgt
     7201 gattggtaaa atggatctga tgaatgcaaa agcacgattt ggtaaagaag taaaagccat
     7261 cgtgccggga atcagtgaag acaatcatgt cgtcttgaaa caagcaagac atccactgat
     7321 cgatcaggaa aaagtagttt ctaatgacat cacaatcgga aaagactatc aagccatcgt
     7381 cattacagga cctaacacag gtgggaaaac gatcacgttg aagactttag gtctgttaca
     7441 actgatgggg caagcaggat taccgattcc tgcaggtgaa gaaagtcaaa tcggtatttt
     7501 tgaggaagta tttgcggata taggagacga acagtcgatt gagcaaagtc tatcgacctt
     7561 ttcttctcat atgacgaaca ctgtagatat tttatcaaaa gtcaatgaaa aaagtctggt
     7621 attatttgac gagctcggtg caggaacaga tccgcaagaa ggtgctgctc tagcaatcgc
     7681 aatcttagat gatcttggta aaaagtctgc ttatgtgatg gcaacaacgc attatcctga
     7741 attgaaagtt tatggatata atcgggcaaa tacgattaat gccagcatgg agtttgatgt
     7801 ggatacatta agtccaacgt atcgtttgct gattggcgtt cctggacgaa gcaatgcgtt
     7861 tgaaatctct agccgattag gtcttgatac agaagttatt gatgaagcga agcagttgat
     7921 gaatgatgag agccaagatt taaacgaaat gatcacagat ttagagaatc gccggaaaat
     7981 ggcagagacc gaatatctgg aaatgcgtca ttttgtttct gaggcacaag aacttcacga
     8041 tgatttgaaa gaagcttaca gctatttctt tgaagaacgt gaaaaagaaa tggaaaaagc
     8101 aaagaaaaaa gcaaatgaag tcgtgtcaga ggcggaagaa aaagcagaaa aaatcattgc
     8161 tgatattcgt aaaatgcaac agcagattgg gcaaggaaat gtgaaagagc atcagttgat
     8221 cgatgcaaag acccaattag caaatctaca tcaagaagaa actttgaaga aaaataaagt
     8281 attgaagaag gcgaaagaac aaaaaacgtt gaaaccggga gacgaagtgt tggttacgac
     8341 ttacggccaa agagggacgc ttttacgtaa aaacgggaat cagtggcaag tagaaatcgg
     8401 tatattaaaa atgaatgtgt ccgaagacga gctgacgcct gttgctcctc aaaaagagcc
     8461 gacacaacga gttatccatg ctgtacgatc tgaatctagt agtcacgtgc cgaatcaatt
     8521 ggaccttcga ggaaaacgat acgaagaagc gctaagtgag gtagatcaat atttggattc
     8581 tgccattcta gctggttatc ctcaagtgac tattgtccac ggaaaaggaa caggcgcact
     8641 tcgcaaggga attactgatt atcttaaaaa ccatcggagc gtcaaaagct ttgagtttgc
     8701 accggcaaac caaggcggaa atggtgcgac aattgttaaa tttaagtaaa gcttactaaa
     8761 ggtaagcaga tggttcgcta tccaagtata gaaatgctat actagtcata gattttaaaa
     8821 atggaggtct ttgatatgtc acaagtaatt acagataaga catttaatga agaaacagat
     8881 aaaggacttg tattgattga tttttgggca acttggtgtg gtccatgtcg tatgcaagca
     8941 ccgattttgg atcagttgga acaagaatat gatgaagaag aattcagaat tgctaaaatg
     9001 gatgtggatg aaaatccaga aactcctcaa caattcggta tcatgagcat tccaacatta
     9061 atgttgaaaa aagatggtca agtagtagaa aaagctgttg gtgttcattc aaaagaacaa
     9121 ctacgtcaaa tgatcgatca atatttataa aaacttacag caaaaataag ctgagggtgg
     9181 gactgaatcc agttccgtcc tcggtttttt tgcggttgag ttaaggttat tcaaattaat
     9241 attttttgag tataattacg ttgtcaggct ggattgtttt tttgacagac atgctataat
     9301 aactttaatt gaatatgaag gggagtacca gatgaacgaa cgaatcaaaa acaaattagc
     9361 tttacttcct gatcagcccg gctgttactt gatgaaagat aaaaatggaa cgatcattta
     9421 tgtcggcaaa gccaaaatat taaaaaatcg tgtccgctca tattttactg gtagccataa
     9481 tacgaaaacg gaacggcttg ttagtgaaat cgaagacttt gaatacatcg ttacagaatc
     9541 gaatatcgaa gcattgctct tagagatcaa tctgataaag aaaaatgatc ctaaatacaa
     9601 cattatgtta aaggatgata aaacctatcc tttccttaag ataacgaatg aaaaatatcc
     9661 ccgtttggtg attactcgta aggtactaaa agacaaagca ctgtattttg gaccatatcc
     9721 cgatgtcggt gcggcgaatg agacaaagaa gatcctcgat cgattatttc ctctgagaaa
     9781 atgcaaacct agtcagacga aggagccttg tctttactat catttgggac aatgcctgtg
     9841 tccttattat ttcgacgtag atccgaaagt ctatacgaaa atcgttgatg aagtgaaaaa
     9901 ctttttaaac ggaggacacg aaaaaatcga gtcagagatc catcagaaaa tggaaaaagc
     9961 tgctgaaaac atggagtttg aaaaagctgc agaataccga gaccagatcc gtgccattga
    10021 gacaatcatg acttgccaaa aaatgacaaa tactgattta ctggatcgag atgtatttgg
    10081 ttatgcgatt gataaaggat ggatgtgtgt ccaagtgttt ttcgtaagac aaggaaagct
    10141 aattgaacga gatgtttcaa tgtttccttt ctataacgaa gcagaagaag atttcttgac
    10201 ttacctcggt cagttttatc aggaaaatga gcatttcatt cctaaagaag tcttgattcc
    10261 tgatacgatc gacaagccca gtgtagaagc tttgttagct acgaaagtgc tgcaaccaaa
    10321 acgaggagaa aagaaaaaat tagttgaatt ggcgagtaag aatgcctcag ttgctttgaa
    10381 tgaacggttt gatctgattg ccagaaaaca ggaacggacg atcggagcag ttgaacgttt
    10441 aggaaacgcg atgaatatcc cgaccccaat acgtatcgag tcttttgata actctaatat
    10501 catggggacg aatcctgtgg ctgcgatggt cgtattcatt gatggaaaac cggcaagaaa
    10561 agaatatcgg aaatataaaa taaaaacagt caacggaccg gatgattatg catcaatgag
    10621 agaagtcatc tatcgcagat attcgcgagt gattcgcgaa gggttgcctt tacctgattt
    10681 gatcttgatc gatggaggaa aaggacaagt agatgtggca aaagatgtct tagaaaatca
    10741 attaggaatc gatattccta ttgctggcat ggtaaaaaac gataaacata aaacaaatga
    10801 gttgctattc ggtacagatt tggctgtagt accgcttgag cgaaattcac cagagttttt
    10861 cttactccag cgaatccaag atgaagtcca tcgattcgca atcactttcc atcggcagct
    10921 acgcagtaaa aacagttttg cttcccgatt agatgaaatt gaagggttag gaccaaaacg
    10981 aaaaaaaatg ctgcttaaag agttcaaatc gttgaaaaat atcacagcag ccacgacaga
    11041 agaattacaa gaaatcggac tgccgaaaaa tgtcgcacaa aacgtatttg ataaattaca
    11101 tcaggaatga ggaaagggca ctttacaaag aaagtaaagt gtcctatcat tttatgtaca
    11161 acaaaaagag cgtttacgtt ttgttggtaa ataataaatc gcttacgtct ggtgtctaag
    11221 gatatatgtg atttcttgaa gaaggagtga agaaatgtta gaagtcaagg atcttgttaa
    11281 aacttttggg aactacacag ctgtcgatca tgtctctttc caaatccccg atggaaaaat
    11341 catggggcta attggccaaa acggggctgg gaagacaacg acttttcgtt tgatcttgga
    11401 ttttttgaca caagaccaag gagaagtgtt atggaatggc catcatcttg gggagaaaga
    11461 ttatgatgtc atcggttatc ttccggagga acggggactt tatccgaaag taacgattca
    11521 agaccaactg ctttattttg cagaacttag agggaaatca agaaaagaga ttgaaccaaa
    11581 aatcgacttt tggatggaaa aatttcaggt aaaagggaaa aagacggata aagtaaaatc
    11641 tttgtctaaa ggaaatcagc aaaaagtcca gctgatcgcc acgttaatcc atgaacctaa
    11701 attgatcatt ttggatgagc cattcagcgg gttagatccg gtaaatgctg aattgctaaa
    11761 agatggaata ttggaattga aaaaacatgg ttcatgtgtc attttttcta gccacaatat
    11821 ggataatgta gaaaaaatct gcgatcattt gatcatgttg cgaaacggaa aaatggtttt
    11881 gaatggaaaa gtacatgaga ttcgtgaatc atttggccgt acgaagttat tcttggaatc
    11941 tggtttaagc caacaggaag tagaagaaat cgaaggcgtg aaaaaagtga tccttcatga
    12001 agacaatacg ttagagatta ccttagaaga tcctgaagct gggaaagaaa tctttactcg
    12061 tgcgactcag ttcggctaca ttcctatgtt taaccagcaa ccgccaactt tggaagagat
    12121 tttcaaattg aaagcaggtg aaatcgatgc gtaagttttg gattattacc aaagatgtct
    12181 atttaaaaaa tgttaagtcg atttctttct tgatcatgat attagtccct tttatcatgc
    12241 tgggtatcat ttatgtagca ggaaactttg ctcaacaaaa tagtgatgtc aataaaatcg
    12301 gcgtattggc tgataatgag caaatcagtc agcaaatgag ccaagtcaaa acagatgatt
    12361 ttgagttcaa agcagttgat tctgaagaaa aagcacaaaa agagctttcg gaagaaaaaa
    12421 tcgatgcgta tttagtagtc tcaactgaga atgataagat aaatgggatg ctttatagcg
    12481 aaaattcatt aggacaaagt acacaactac tgatacaaca acaactgact ggcttgcaat
    12541 cgatgcttcg agcgagtcaa ttaggcattt caccagaaga agtagctagc ttgagccaac
    12601 aagcaagttt gactcgacaa aaagtcaatt ttgattcaaa aggaaaaatg acagtcggag
    12661 aagataactc gacagtccaa tacgtcgtaa gttatgtagc aacgattgtc ttgttcatta
    12721 ttattttgac gtatgcgcag atcattgcac aagagatcgc ttctgaaaaa ggcacgcgga
    12781 tcatggaagt gatcctgtcc agtacgactg cacaaaagca tttttacgga aaattaacag
    12841 gtgttttact tgttgcatta acgcaaatgg cattatacgg tattattttt ggtataggat
    12901 acagtcaatt caaagatatg gaaatggtca aaaatatatt agacggtatc tcattggata
    12961 acttgattgg accattcttg tggtattctc ttctattcat gatctttggc attttgattt
    13021 tttctgtttt agcagcttta tgtggctctt tggtcaacaa agcagaagat acagcaaaag
    13081 caattttgcc agtgacttat ctatctctag gcggatacat gcttggattg atactaggag
    13141 cttcagatcc aaacaatatc gttattcgga tcacttcgta tattccattc ttatcttcat
    13201 atattatgcc gattcgctta gcaaatgaga cagtcggtat gggtggagca ttgatttcct
    13261 tgttggtact tgtagccgta acattcttat tgatgatctt gtctgcaaac atgtacaaat
    13321 cgaatgtact cgtttatagc gaaggcggat tatggagttc attgaaacaa tcgatttcaa
    13381 ttatgaggaa tgaacgaaaa aaagcataaa acaatcctaa aatacagaag gaagctggac
    13441 atctattgtt cccagcttct ttttgtatag acctatttta tttattttac tatattggat
    13501 atatattcat tgaaaatagt gtataacaac tttataatat tgagtatacc aatttttgga
    13561 ggcgttttaa tggaccgtta tttagattta ctttcagaaa aattcccctc aacggaggat
    13621 gtcatcacgg aaatcatcaa tctcgaagca attttgcagc ttccaaaagg aacagagtta
    13681 tttttgagtg atatacatgg cgaatttcct gcttttgatc atattctgcg tatcggttca
    13741 ggaaatctta aagaaaagat caaagacctt ttttctgaac aaatgacgga aacggatatc
    13801 agccaattca ctatttttac ggcttatcca gaatatgcat taacgactga ttggtacaaa
    13861 gaacaagata aatcgcagct gatccatcat ttgatcgatc ttttacggtt tacgacagtc
    13921 aaatataccc ggtcaaaagt tcgaaaagga ttgccgaaag aatatagcta tatcattgag
    13981 gaactcttgt atatcgatga ccgtatcaat ggaaaaaaag actatgtaaa aaaaatcatt
    14041 gaacaacttg ttttgatgaa ggaagaagaa aaatttttaa cgaaacttgc acagacgatc
    14101 caaaaatctg tcatcgatca tttgcatatc gttggagata tttttgaccg aggaactcaa
    14161 gcagccaaag tgatggatcg aatcatggat ttttcttctg tagatatcca atggggaaat
    14221 catgatattt tgtggattgg tgcgtattgc ggctctgagg catgtttact gacactttta
    14281 cggattgcag cacgctataa ttatttattt gaattagaga aagaatatgg gttgaattta
    14341 cgtccattgt tttcttttgc tcatcaaact tatcagaaga atccagtatt tgcgcctaaa
    14401 aacagtaaaa atctatccga aagagagcaa gaagaattag aaaaaatcca tcaggcactt
    14461 tctatccttc aattcaaatc agaacatcag ttattggatc gtcgacctga atttaaaatg
    14521 gacgatcgta aattgctgga aaaaatcgac tatgaagcat caacgattga tttagaaggt
    14581 cagctctatg agttgaaagg aacttgtttt caaacgatcc agccagatga tccaaaaaaa
    14641 ttcttggaag aggaacaaaa agtgattgat agtttgatgt attcattcca gcactctttg
    14701 agaatgcaaa aacatatgac gtttttgatc gaaaaagggg gaatgtatct cacgaacaat
    14761 cagcatgtgc tgtttcatgg ctgtatacct gtagacgaga agggacagct cttagcattt
    14821 gaattagacc aatcttatcg cggaaaagaa ctgctgggct tttttgaaaa acagataact
    14881 gaaagcgcaa aagatctgac tgcaaaagaa gatttagcaa ccgatttgat ttggtatgcg
    14941 tggagtgggc cattttcgcc tttattcggc aagagtaaaa tggcgacttt tgaacgttat
    15001 tttctcacag agcctcatac gcacgtcgag aaaagcaatc cttattatca cttgcgagat
    15061 gaagagtggt tcagtgaaaa aattttagca gaattcgaca caaaagaaga agggtcagca
    15121 atcattaatg ggcacacgcc agtaaaagta aaaaaagggg agtcaccgat caaagcaaat
    15181 ggaaaagtat ttgtcattga cggaggttta tccaaagctt atcaaaaaac gacaggaatt
    15241 gccggttatt cactattatg caattcttat ggtttccaaa tcgtgacaca ttatccgttt
    15301 ttgcctattg aacaacaatt tgcaaaaaaa tccgaccaaa caaatgtgaa aaaagtaatc
    15361 gaacagcaac taccacgcaa actcaatcgt gatacaacaa aaggtatgga actgcagcag
    15421 cagattaccc agctcaaaag gctgttggac tatcgaaagg acgattgaga agaattccag
    15481 tgaacattgt tccatagact ggagactgag acaaaagtcg tgtcgcagtc tctttttccg
    15541 gataaacggt gttcttctgc caactcctcg gtcacaagtc acactattca acaaacatga
    15601 aaagctgttt tttgaaattg ctccttgtga ctcagagttg aacggcaggc tcacgacctc
    15661 ttggtataaa cggtgttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat
    15721 gtgaaaagcc attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacaa
    15781 ccccctcaga aagtctatta aaattcattc acaaagaaat cgaataagcg ctgttcagat
    15841 ggatgtacac ggtgggtaag ttccggatgc cattgaacac cgaggatttt atgcatatca
    15901 tctttgcttt gaaccgcttc aacgagtcca tcgttagaaa aagcgatggc ttttaataaa
    15961 ggtgaaagat ctttgactgc ctgatgatgg taagaattca caaaatatgg atcggggaga
    16021 aggttgctta acaaactgtc tgaaacaatc ttgacttcgt gagtcgtaaa ttgcggggca
    16081 gtgggctgtt gttcatgttt tactgaccac tttggatata atgaaagatc ttgataaagt
    16141 gtaccttcca aaacgacatt caatagttgg agtccccggc agactgcaaa aattggtttt
    16201 ctttgtttca atgcttccaa gatcagtgcc tgttcaaatt ggtctcgttg aatatttgtt
    16261 tccgttagct ttggatgagg atcttccatg taaagttgtg gactgacatc ttgaccacct
    16321 gcaagaataa gtttgtcgat ttgtccaatc agttgaggag ctgaatctgg agacatgact
    16381 ggcagaataa gaggcaatcc ttgagcatct aaaactgcat ccacgaatcc ttgcggtgta
    16441 tagctcactt gatttccttg gaaagtatct gttgctcgaa tcagttgatt tccggcaatg
    16501 ccgatgattg gtttcatttg tattccccct aaagtagact tattgtaaca aggatttaaa
    16561 aaaaattaaa ctatgtaaga cttgccccta tacttatctg ttcttgctgt gttactatct
    16621 gtaaagagaa atttcgtgtg aaattcatga cgatagttga aaatattgct taatttgtta
    16681 taataaaaag taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca
    16741 aggggtgaga aggacatttt attgaagggg cctgcctata ctaagttatc tttagaggta
    16801 catactttca atgaaaaaag aaagcatgta cctctttttt aaatcgaaag gaagtgaagc
    16861 aagtgttaga agctttggaa caaatccgaa aagcagaaga agaaaatgaa aagcgtgctc
    16921 aaacactgcg gcagcagctg aagatctatg caacagaaaa agagcaggaa ttggaaacga
    16981 tccaaacaaa ctatcgacaa caactaaaac agttggtgga agaaaaagaa caatttgaaa
    17041 tacaagaatt agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa
    17101 ctttgaaaga tcaatatcaa aagcatcaga gtcaagcgat cggtgcgatc attgaaaggg
    17161 tgaaagaaac ttatggccgt cactaagatg gaaaaagtga ccctcatttc agataaaaga
    17221 aatcaggaga ggattttaca agcagtacaa ggaatccaaa acgttgaaat ccgggatctg
    17281 tatcaaaata gaacaaacaa ccagtgggtg gagaagtatt ttccagatac cacgattatt
    17341 gataaagaag caaaattaag cgctttaggt aatcggttga aagacatcca agaagctatc
    17401 caattcatac aacaccacgg cgatagccgt caaaagaaac tccatttgaa acgtcaagaa
    17461 ctttccttac aagatttgga agccaattat tccgaagaaa atttcataaa taaattgaaa
    17521 gaagtccttg atttaaaaga aaagtgggaa caactaactg aagaacgtga tcgtttagaa
    17581 gaagaggaag aatggcttct tcattggcaa cagctggata ttgctccaag tatgtatgca
    17641 agtaagacga ccatttttga gatggggact gtgagtgcag caaattttga aagtttcaag
    17701 gaagaactga gtaagcttga tgacgtttat atggaagaaa tagattatac gccaaaacaa
    17761 gtttatcttt cgtatgttgc tttaaaaaaa tcagttgctg ttatagaaga aattgctggg
    17821 aaatacgggt tcaataaaga aacgtatcct tatgaacaaa atcctaaaga aaaactgcaa
    17881 gagaccaagc agcaattatc cattgttcac gagcagcaaa agaagataac gacagcgtta
    17941 ggtcagtgca gtggctatat ccaagaattt gaatgggtag aagaagtcac acttgccgtt
    18001 gcagaacgag aaaaaataaa agaacggttt atccatgcga cttatctgat cgttttgcaa
    18061 ggttgggtag atgcagaaga aaaacagaat ttgctgcatg tgttaaacga aaccgtatca
    18121 gaagaagata tatacgtcag tttcgaaaca ccaaactcag aagagattca gcaggaagtc
    18181 cctactaaat tgaaaaacca tccgcttgtc gaaccttttg aattgctgac tgaaatgtac
    18241 agtctgccaa aatatgaaga agtagatcca acaccttgga tgacaccatt ttatcttgtc
    18301 ttcttcggga tgatggtcgc agatgttggt tatggtttgc tgatgctcat tgggacaata
    18361 ctagctcaaa aactacttgt tctaccacgc gggatgaaac gtttcgtaaa attctttgag
    18421 attctctcga taccatcgat catctgggga ttgatctaca gttcattttt cggacagtca
    18481 ttaccaaaag aactgtttgg catccgcttg cctttcccaa tcctatctac gacggatgac
    18541 gtcaatacga ttttgatttt atccgtgatt ttcggtctga tacagatttt ggttggatta
    18601 tttgtggcag caaaggaaaa tatcaggaga aaagattact tgagtgctgt cagtgatgga
    18661 tttgcctggc aagggatcct aattgggatc gttattgcgt tggttggtgc catgttgata
    18721 aaaaatacag catttgttta ccttggcgct gggctatcga tcattgctgc cttatgtatc
    18781 ttgatcgttc cgattatcca aacgccgtct aaagtcaaag gtgctgccaa aggtgcgtat
    18841 aatctttacg gactgaccgg ctatatcggt gatttagtca gttatacacg tttgatggca
    18901 ttgggtattt caggcggaag tattggagcg gcattcaata tgctagttgc tttcatgccg
    18961 ccagctgctc gattcagtgt agggatcctt ttgatcatcg ctttacacgc actgaatatg
    19021 tttttgacac tgctaagtgc ctatgtccat ggcgcacgat tgcagtatgt cgaatttttt
    19081 gggaagtttt atacaggagg cggacgtgcc tttgatccgc tgaaaacggc ggaaaaatac
    19141 gtcaacatca atcacaagaa aaaaaacaat aaataattgg aggattttta tataatgatg
    19201 gattacttaa ttagtcaaaa tggtggaatg gtatttgcgg tattagcgat ggcaacagcg
    19261 acgatttttt cagggatcgg ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca
    19321 gcattgacaa caagtcaacc cgaaaaattc gggcaggctc tgattctgca attgcttcca
    19381 ggcacgcaag gtttgtatgg attcgttatt gcctttttga tttttatcaa cttaggaaat
    19441 gacatgtcag ttgtacaagg actgaacttt ctaggtgctt ctctgccgat tgcttttaca
    19501 ggcttgtttt caggtattgc ccaaggaaaa gtcgcagcgg caggtattca aattttagcg
    19561 aaaaaacctg aacatgctac aaaaggaatc atttttgctg cgatggttga aacatacgcg
    19621 attttaggtt tcgttatttc cttcctatta gtgttgaatg cctaagcata aaggaggaaa
    19681 agcagatgaa cgccattgaa aaaatcatct ctcagatgaa cgaagcagca gagcaggaaa
    19741 gagcggcgtt agaacaagaa gagcgaatga agatcgatca aaactttgaa caaaaacgta
    19801 cgcaagttga aacagaacat caaaaacaaa aagaaaaaca aatcgaatta ctcgaaaaaa
    19861 aataccgtca gctaaggaat cgccagcaag tagaagtacg acaagaaaat ttgaatgcga
    19921 agcaggaatt tttaagacga ttgtttgcag atgcggtaac tgaaatggaa aattgggatg
    19981 aatctgaaca aatccagttt atcaaaaatg cattgtattc attacctttg acaggaaaag
    20041 tagcattcat tgctggagaa aaatcagcag cctatctttc tcagacgttg ttggatgaat
    20101 ggaacaatga acttcccttt atgatggttt taagtgatga aactgttgct gatcaagcgg
    20161 gttttctgat aaacgatcaa ggagttcagt ataattttct tttcagcagc cttgtacaag
    20221 atatccaagg aacgatgagt tttgagattg ccaatcaatt attcgaataa ggagggatca
    20281 gtgtatggaa taccacgaac taaatccctt gattcgcgga agagagcttg aacttttgtc
    20341 caatgagaca tttgaacgga tgattcaaac aaattctgtc gaagcacttg gtgaaatatt
    20401 gaaatccacg atttacagtc catatattta tgacggatat gaatatgact tcgaacagaa
    20461 tcttttaaag gagcagagcc gtctatttcg ctggttaaag gaatcagcac cggagcctga
    20521 aatcgtttgg atctatacga tgcgttatac tttccataat ctgaaggttt tgacaaaagc
    20581 tgagatgaca aatcaaaatc tggaccattt gtatatcgac gatggatttt attcattaga
    20641 aaccttgaag gatgccattc atacgcaagt ttcaaatgaa cttcctgaaa gcattatgga
    20701 cagtatacga gaagtacgtg attattttga agagtctacc attcttcagg gaattgacgt
    20761 gatttatgat cgacagtttt tgacagagca gcgacggctt gccgataagc taggatatca
    20821 agaattgctg acagaagtca ttgcttttat tgacttgacc aatatcacaa cgatggctcg
    20881 aggaatattg cagaaaagaa catatgggtt catgacgacg gtactttcca gctcgggaag
    20941 tatcgaaaaa gaagtatttt tgaactttgt agataaagat atggaagatt acacgaattt
    21001 tctattagag acagattatg ctgattttct gcgccctgtc atccgtgagg gaaatattga
    21061 tctcgtaaag cttgaacagt tgaaagatga ttatctaagt tcattgtatc aagaagcaca
    21121 aacccaggct ttcggaccac tgccactact agcctttttg aacgcaaaag aggtagaaag
    21181 caaaaacctt cgtttactgg ttatcggcaa aagaagccat ttcgaatcag aaacaatcag
    21241 aaagcgggtg agacaagtct atgacgcata agatcggtgt agttggcgac aaagattctg
    21301 ttttgccatt caaattgttt ggctttgatg ttcgttatgg tacaaccaag caaaccgttc
    21361 gtcaggcaat tgaagaaatg gcaaagaatg agtatggcgt gatttatgtc acagagcaat
    21421 gtgcagcatt gagcccagaa acgatcgaac gttacaagga acaaatgata cctgctatcg
    21481 tattgattcc caatcatcaa ggatcattag ggatagggct ggaagaaatt caaaaaaacg
    21541 tagaaaaagc agtaggtcaa aatatcttat agtatgaaaa ggagatagaa attttgcaaa
    21601 ttggaaaaat cataaaagtc tccggacctc ttgtaatggc tgagaacatg tctgatgcca
    21661 gtatccaaga catgtgtcta gtgggagatc taggagttat cggagaaatt atcgagatgc
    21721 gtggcgatgt ggcgtctatt caagtatatg aagagacttc tggaattggt ccaggcgaac
    21781 cggttcgctc tactggtgaa gctttatctg ttgaattagg accagggatc atttcacaaa
    21841 tgtttgacgg aatccaacgg ccattagata cctttatgga aatcacacaa agtaatttct
    21901 tagggcgcgg agttcaattg cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa
    21961 cagtagaagc cggagaaacg gtgagtgctg gagatgttat cgggatcgta gaagaaacaa
    22021 aagttataaa acataaaatc atggtgccaa atgggataaa aggaacgatc aaaacaatcg
    22081 aaaaaggttc attcactatt gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca
    22141 aaaatttgac gatgcttcaa aaatggccag taagacgtag tcggccaatc aaagaaaaat
    22201 taaacccgga tgcacccatg atcactggac agcgagtgat tgatacattc ttcccagtga
    22261 caaaaggagg agcagcagct gtacctggtc cttttggtgc agggaaaaca gtcgttcagc
    22321 atcaaattgc aaaatgggcc gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg
    22381 gaaatgagat gacggatgtt ttaaatgaat tccctgaatt gattgaccct aatacaggag
    22441 aatcgttgat ggaacgtacg gtattgattg ctaatacatc aaacatgcca gtagcagctc
    22501 gagaagcttc tatctatacg ggaatcacaa ttgcggaata tttccgtgac atgggttatg
    22561 acgttgctat catggcagac tccacttcac gatgggcaga agcacttcgt gagatgagcg
    22621 gacgtttgga agaaatgcct ggtgatgaag gttatccagc ttatcttggt tcacggcttg
    22681 cggaatacta tgaacgatct ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa
    22741 gtataaccgc aatcagtgct gtatccccgt ctggagggga tatttcggaa ccagtcacac
    22801 aaaatacatt gcgggtcgta aaagtattct ggggtcttga ttccagttta gcccaaaaaa
    22861 gacattttcc ttcgatcgat tggatacaaa gctattctct ttattcaaca gaagtaggga
    22921 agtatatgga tcatatcctt caagctgact gggcatccat ggtcaccgag ggcatgcgta
    22981 ttttgcagga agaacagcag ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt
    23041 cagataatga ccgtctgaca ttagaagtgg caaaatccat tcgtgaagat tatttgcagc
    23101 aaaatgcttt tgatgatgtc gatacattca cttcaagaga gaaacaattc aaaatgttga
    23161 agatgatctt gacctttggt gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa
    23221 gtgagatcat ggacggtacc gtatcgattc gtgaaaggat cggacgaagc aaatatatcc
    23281 cggaaacaga attagaaaaa ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa
    23341 cgatcgtttc agaaggaggc atgagcaatg attaaagaat atcgtacgat cggtgaagtc
    23401 gtcggtccat tgatggcggt tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa
    23461 gtccgtatgc aaaatggcga gattcgtcgt ggacaagtat tggaagtcca agaagataaa
    23521 gcaatggtcc aaatattcga aggaacaagt ggtatcaatt tacgtgactc ttctgtacga
    23581 tttttaggcc atcctttaga actaggtgta tcggaagaca tgatcggtcg agtattcgat
    23641 ggtttgggac gaccgaaaga taacggacca gagatcctgc ctgaaaagca tttggatatt
    23701 aacggagaag taatcaatcc agtagctcgg gattatccag atgagtttat ccaaacaggt
    23761 atctctgcaa tcgatcattt gaatacatta gttcgtgggc aaaaattacc agtattttct
    23821 ggctctggtc ttcctcataa agaattagct gctcagatcg cgcgacaagc aacggtactc
    23881 gattcatcag atgatttcgc cgtagtcttt gctgctattg gaatcacttt tgaagaagct
    23941 gaatttttca tggaagattt tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg
    24001 aatttagcaa acgaccctgc aatcgaacgt atcgctactc ctcgtatggc tttgactgct
    24061 gcagaatatc ttgcttatga aaagggaatg catgttttgg ttatcatgac agacatgacc
    24121 aactatgcag aggcattgcg agagatttct gcagcacggc gagaagttcc aggaagaaga
    24181 ggatatcctg ggtatcttta cacgaactta gctactttat tcgaacgagc aggaagaatc
    24241 cgcggactaa aaggatcggt tactcaaatt ccgattctaa caatgccaga agacgataaa
    24301 actcatccaa tccctgactt gactggttat attacagaag gacagatcat cttgactaga
    24361 gagctttaca agagtgggat tcagccacca attgatgtac tcccgtctct ttcgcggtta
    24421 aaagataagg gaactggacc cggaaagaca agagaagacc atgcagctac gatgaatcag
    24481 ctttttgcag cctatgcaca aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa
    24541 tctgctttat ctgatgtaga caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa
    24601 tatgtcaatc aaggattcta cacgaaccgt tcgattactg aaacacttga tctagggtgg
    24661 gaactgctat cgatgttgcc aagaaccgaa ttaaaacgaa tcaaagacga catgctggac
    24721 aaatatctgc cagaagggaa gtgagttctg atggcacgat tgaatgtgaa tccgacccgc
    24781 atggaattga ctcgattgaa aaaacagttg acaactgcca caagagggca taaattatta
    24841 aaagataagc aagatgaact gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag
    24901 ctgcgtcaag aaatggaaga gtctatgaca gaagcaatgt ctgattttgt attggcaaat
    24961 gcttcgatca cggaaccttt catcgaagaa ttatttgttt tgccggcgga aaacgttgat
    25021 ttgtctatcg tagacaaaaa tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat
    25081 gatgaacagc tgacagacac acctttggaa tacgggtatt tgaattccag tgccgaactg
    25141 gatcggtcaa tcgatcggtt tactaccctg cttccaaaac ttttggaact gacagaaatc
    25201 gaaaaaactt gtcaactgat ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca
    25261 ttagaatata tgacgatccc tcaattagaa gaaacgatct attatattcg tatgaagctg
    25321 gaagaaaacg agcgcgcaga agtaacaagg ttgatcaaag taaaaaatat gggtaccact
    25381 gactaagtgg tatctttttt taaaagagga aaacgatgat taaacaaagc aaacaaataa
    25441 agaaacgttt gccggctgtt cagcttctcg cattaggatt ttttctcctg attttagttg
    25501 gcggcggcct acttacccta ccatttttta gtaatagtgg gcatgggaca aattttatcg
    25561 atgcgctttt cactgcaact tctgctgtct gtgtaacggg attgacaaca ttgaatacag
    25621 cagagcattg gaacagtgct ggtcaatttt tgatcatgct tttgattgag atcggcggtc
    25681 ttggttttat gatggttccg attctatgct ttgcattgat aaagaagaaa gtcagtctca
    25741 gcacacgtat tatattaaaa gaagcgctga atctagaaga agtatcaggt gtgatgaagc
    25801 tcatgcttta catcctaaaa ttggcctttg tgatccaatt gattggtacg gttgctttat
    25861 cttttgtatt tgtgcctgaa tttggctggt tgaaaggtat ctggtacagt ttgttccatg
    25921 caatctctag tttttgtaat gcagggtttg atttgttagg agacagttta gccaatcacc
    25981 agcaaaatgt ttatttgatc atggtggtat ctgctttgat catttctggt gggttaggct
    26041 tcatcgtatg gcgtgattta ctggaatatc atcagaaaaa gaagatgacg attcattcaa
    26101 aaatcgcatt aaccgttaca ggtacccttt tattaggcgg attcgttgtg ttctttttca
    26161 cagagcgcaa tggggcaacg ctagtggaag ggaatatgtt tcaacgattg gcaaacacct
    26221 tttttatgag tgtgacacct agaacggctg gatattattc agttgattac ctccatatga
    26281 gccatgcagg tctgatttta acgatgtttt tgatgtacat cggcggaaca tctgggtcga
    26341 cggccggcgg attgaaaaca actacgttag gtgtgttgct gatccagatg cgttcgatgt
    26401 tgaaaggacg tacgagagcg gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc
    26461 gggcacttac tttatttttt gttacgttga cgttgtgtgt agtcgccatc atgattttat
    26521 cggtgactga aacgatacct aaaacttctg gcatcgaata tattgctttt gaggtcttct
    26581 ctgcgtttgg tacagtaggt cttacgatgg gattgacgcc agacttgact gcaataggga
    26641 aattgatcat tatagcgctt atgtacattg gacgagttgg tgtattgact gtggtcttct
    26701 ctctgatgac aaaagcacaa agaacacaat caaaatacaa atatccagaa gaaagtgtca
    26761 tgattggata atttcatctt ctgacagaaa cctagtgatt tagtaaaata agaaaataaa
    26821 ataatttttc ttgcttcttg gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca
    26881 ctacccgttg ttgaacctcg ggtagtgcct cttttttatt gaaaggaatc aataagattt
    26941 gattccttta tttatcaata ggttataatt catatttttt tattttttct tttacgatac
    27001 ttagtccaga agctaaaatt tcaaggttat tagaggatag ggaaattcga acgaatgcat
    27061 ctggttgcgc acgtgtagta aagcgtctgg aatgataaag tcggataccg ttagcaagaa
    27121 agtcattctc aattatctta gggtcaatct caggatcaac tggaagaatt cgatagtaag
    27181 ggacacgatt cgttggtttt tctactttga aaagagaatc aaaaagctga ttggctcttt
    27241 ttgtaagact taatttagtt tctaagatcc tatttgcttc atttgaatgg aggatttgag
    27301 tgatgacttc cacatctaat ccagaagttt tcacatttat attaaaaatt gcttgttcta
    27361 tcttgctttg gacatgtact ggaactacaa gataagcaac tcgcaatcca gaacaaataa
    27421 attttgtcat tccagctaag tagattgttt ggttaggtaa taaattttgg aaaggaggaa
    27481 tgatcgtatg ttgatagtaa gttgttaaaa atgaatggat atcatcttca ataacccata
    27541 gaccttcttt ttcaatcact tcggctaatt gctctcgtct tttgtgagag atttgaaacc
    27601 ctataggatt attacaagaa ggcataagaa agacaccatg aatcttcttt tttctacatt
    27661 cctgaatcaa tatatcaggt cgcattcctt gctcgtcaaa atcaattgga acgatctcta
    27721 aatgatataa ctgagccaat tcaatgaaat tagcgtatgt atatcggtct gttgctattc
    27781 tatcacctgg agagaaaagt gcagaaagtg tgatagccaa tccattttga acaccggaaa
    27841 caatagatag agattctaag gacggaatga ctccttgagt ttttaaccaa tttgctgcag
    27901 tagtcaattg aaaatttgtg cctttgggat cttggtaatt aagcaaacca acaagatttg
    27961 ggttattact agttgattga ataaacggga caacgaattt attacattct tcaaaagaac
    28021 taaccagacc aaaatcaatg atatgttcgg ttacttcatt ggtagagatg gtgatggact
    28081 caaagacatt gggagaaata aatgtgccac ttccaatatt cgtatacagc aaacctttct
    28141 caattccata tttataagcc ttgccaacgg tagtaaaatt taaatctaaa taatcagcta
    28201 gctctctttg ggagggtaac tgagtgtttt tctgtaattt attctttaaa atatcttgtt
    28261 ctaattgttg aatcaacgat cgataataag gacgcttcaa tttttgtttg tctggtcgcc
    28321 aagttaacgg gtaattgtca taagaattaa taggcatttt ctccactcct taaaattgta
    28381 taccatacaa ttttaccatt gattgtattg attttagaag ttaatctttt gtgaggaggg
    28441 aattgtctat gaaaattatt aattttgagg aagtagtagc ttcttttagt tttgaagaag
    28501 cgattcaagt aatgaaaaaa tgtttttttg attatgaaag tggtaaaatc agccaaaatc
    28561 ctcgattggt ggaaatttta cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg
    28621 cttatttggg ggagaacaga tattttggca caaaagtcat taccgctttt ccggataatc
    28681 atcagtatca tttaccttct catttaggag agattttctt gtttgatgct caaaacggac
    28741 tacccaaagc attgataaac gcaaatgcag tcacctggat tcgaacagct gctgtatctg
    28801 cgctggcaac agattactta gcaaatccgc atgcagaaac acttgcttta attggagctg
    28861 gtcaacaagc attgtcgcat ttgactgcga tttctaccat tcgtcccatc actacagtgt
    28921 ttgtttacga tattttagaa gaaagacgaa atacttttat cgctaaagct aaaaaacagt
    28981 atcctaaaat aacgtttata aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa
    29041 tcatttgtac gttgacctct agtaaagagg catttcttca aaaagagtgg gtaagggaag
    29101 acgcacatat caatgctgtg ggcacattta ctcctgatac aagagagata agtacatcgt
    29161 tgatggaaac ttctcaaatc tatgtagatg attactcagc tgccttaaca gaaagcgggg
    29221 acctgcttat agccatggca gaaggagatt tagaggaatc ttgtattgtt ggtacattag
    29281 gcgaattagt ttctgagaaa aaaacatttt ataaaggaga atcgaggccg acgattttcg
    29341 acgcagttgg tctagctatt gaagatcttt gttgtgcaga atatatttat aacaaattag
    29401 agaaaggggc tttaaaatga acttagatta ttacgtagtt gatgcatttg cagatgaggt
    29461 gttcaaagga aatccagcag cggtttatgt gctagaagaa tggctgccag aaggtacgat
    29521 gcaaaaaatt gccattgaaa acaatttatc tgaaacagca tttacagtga agaaaaacca
    29581 agaatttgag cttagatggt ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac
    29641 attagcaaca gcatttgttt tatttaacta ttacaaaata cctgatgaaa cgattaaatt
    29701 ttctactcaa agcggtaatc tatttgtgac aagaattaaa gattattatt acatggattt
    29761 tcctagtatt atgccgaaaa aagtgccgat attgtctgaa tatgaagaag cgattggggc
    29821 taagattaaa gaagcctact tagcacgtga tttatttttc gtattagaag atgaaaaaac
    29881 ggtggctaaa ttgcagccag attttacagc tatcaaaaat tttgaattag gaataggagt
    29941 catcgtgaca gcagaaagtg tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt
    30001 aacaatcaat gaagatccag tatgtggatc agcacattct aatcttattc cttattgggc
    30061 agaaagaatg aataaaacaa aattgtcagc ttatcaaatg tcatcaaggg gcggggatct
    30121 agaatgtgaa ttaaaagaag atcgtgtgat aataggagga aaagcagttt tgttttctaa
    30181 aggtacctcg tttatttgat ttctttcaaa tgaattaaaa ataaattggt ggagagtggc
    30241 aaccttatag ctacaatggt tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag
    30301 tttgcgtctc attttaatta ttggtgtttg ttttttattc aatcaagagt aaaatgcccc
    30361 atactaacta tcctcggatg acttcgattt tataaccatc agggtcaatg acaaagtagt
    30421 aagaaggcgc agtgccaggt aatcctttta agtcagtaat ctcaagacca gcagctttat
    30481 gttgctcgtg taatttttct agatcatcag ctgctatagc gatatgacca tagccgtctc
    30541 ctagatcata cgcaccatga tcatagttgt aagtcaactc taattcatag tcatccccag
    30601 gaagtgtcaa ataaacaagt gtaaatttgt tttctggaaa atcacggcgg cggctttctt
    30661 caaaaccaaa tgccttttga taaaattcga ttgatgcttc aagatctttc actcgaacac
    30721 atgtgtgagc cattttcata gattcatctc tccttttcct tttactcaat cataacaaaa
    30781 agaagtaagg aaatagttga tttgtgcttg ttcatgagaa atttctcatg taaaatggat
    30841 agaaaataaa actgttaagg tggaaactat tcatggataa accaattttt ggtgaaaaaa
    30901 agcaatttgc tgattatcaa acccgttatg cagcatatat tgtcatagaa agagatcagc
    30961 agattgcagt aatcgaggct ccaaatggtg ctttttttct tcctggcggg gaaattgaag
    31021 gagaagagac gaaagaagaa gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg
    31081 cagaaatttc ttcttatctg ggagaagctg atgagtattt ttactcgaat tatcgtgcaa
    31141 cttattatta taatccagga tatttctttg ttgctgattc ttggaagcgt ataggagaac
    31201 caactgaaaa gaccaataaa atttggtggg tgagccctca agaagcgctt gtgaaattga
    31261 aacgtggtag ccaccgatgg gcagtcgaga gatggatcga aaaaaataaa aactgaaaga
    31321 aagcgagtag atcagctttc tctcagtttt tttatttatt acagtacata ttttttgatt
    31381 gcttcagcta caccgtgctc gtcgtttgat gaagtgatga catttgctgc ttctttgaca
    31441 agagaaacgg catttcccat tgctactcct agaccagcat attcaatcat ggataagtcg
    31501 ttctcgttat ctccgatagc catgatttca tcttgatcaa taccaaggtg ctgtgcgaga
    31561 ttcgcaactg ccgcaccttt acttgcttct ttatttagta cttcgaagta atagggcgca
    31621 cttttgaccg ttgtgtattt atcacggaaa gtttgcggta aacgcgcaat tgctgcatct
    31681 aaaatttctg gttcgtcgat catcatcatt ttgacgatgt tcatgtctgg tgtcatttct
    31741 tcaggtgttc gatattttag cggcatcttg actaaacttg cttcgtggat cgtataagcg
    31801 ctgatatctc gattaggcgt gtagatcgct ttgtcatcga tcgaatggag atgactgccg
    31861 acacggcgtg ccattacttc gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg
    31921 atgattttac ctgatttcgt ttgttgtacg agtgccccgt tataagtaat aacgtaatca
    31981 ttatcttgaa aaaggttcaa ctcgtttaac tgttcctgga cacctggtaa gggacggcca
    32041 gtgcataaaa cgatcgaaac accttttgca acagcagcgg caatcgcttc ttttacttct
    32101 tttgttactt ctcttttctc atttaataaa gtaccatcga tgtcgatagc gactagtttg
    32161 atagacataa tttcctccaa tatattttaa tttatttcaa tcaatgcgcc attacggata
    32221 tggctggcaa attcttgata agtttcgtca aataaatcat aatgatcacg caaggacgga
    32281 tcaaccattt cttttggaaa gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca
    32341 gcaacaagct gactaacctg tgataattct atcaaagttc cgtcatttcg caaaatctcg
    32401 atttgtgtgc gatgtctgcc ttgttctggg cgataaaaat cataaggtag atcgtagcta
    32461 gaattcacag cagtgtaata aacaggatta tatccgacct tttctactaa caaagtgagt
    32521 tcttggacta attttgaatc ccggttgcca gaaaaagtag cagatttcag tggtttacga
    32581 ttcaaaaaac gtttagctaa atcatttaaa ataggatcag cagattcaga ccattgagta
    32641 aagtaagtgc ccaagacccc atcatcaagt ttgaggtatt cttctaaagt aaagcttccg
    32701 tcaagaaatg gcagaagtaa ttgagaatgg agttcaaaac tgctggcatc ttcttgatac
    32761 aactcgtgtg cgcgatgaag cagatgctcc aaaattactt ccataccacg ggaaacagga
    32821 tggaagtaca cttgcacata catttgatat cggctaacga tataatcttc cactgcatgc
    32881 atgccactca tggaaaaagc gatcccgcct tcataaggac gaatcacacg taatattcgg
    32941 gtaagatcaa atgttccata ttctgttcca gtgaaataag catcccgaag caaatagtcc
    33001 attcgatctg catcgatctg actagaaatc atttggacaa cttgtgggtt ggggtaagtt
    33061 ttttggatca cactagctac cttttcagga aaaccttctt ctactcggtt aagaatctga
    33121 tacacttctg tttctggaga agtgatgatc tctacagtga tggcttcatg attcgtatgg
    33181 aagatatgtt caaatgtatg agagtaagga ccatgaccga catcatgcaa cagtgccgca
    33241 cataaagtca ccagccgttc ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa
    33301 aaatttcgag aaaagatatc gcagattcgt cttgaaatct cataaacgcc taatgaatga
    33361 gtaaaacggc tatgttctgc tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaatg
    33421 cgtcgtagac gttggacttc tttcgaattg atcaaatcta agattacttg atgctggaca
    33481 tggatatagt tatggactgg atcacgaaaa actttttcca taggcagcat ttgatattta
    33541 taaggggttg tcataatgta gctcctttat gttattacgt tgttccattc tctggatttg
    33601 gttgttccat tgctccagct ggatttcttt ttttagccag tcggtagaat cctgtaaatc
    33661 acttgcagaa tacagctttt caaaagcttt tgcaaatgct tctttgactt cttttacaaa
    33721 aaggggttgg tcaagcagtt cttcaagtgt tgccattgta gcaggatcca ctgcaggata
    33781 gccatttgta ccaaagtctt cttttaaagc agattgataa aatcgcttca tgacttgccc
    33841 acgtttttcc tgattaccgt tgacacttaa gtacatcata acagaaatcc catttttgat
    33901 ccgacgctga gcgataccgg caaatttttt gccatggatg cttaaatcat aagttccagg
    33961 acaataagaa tgggtgattt cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt
    34021 caataggagc atttgctcgt aagcgtcatc tatggttatt ttttttgtgt cactttgggg
    34081 gaaaatcaaa gaaatattca aaattccttc atctgatacg atccctaatc cacctgaatt
    34141 tctgatcact ggagcgtaac ctgcatcttc cagtgtttgg atcccgtcac ttagaaaagg
    34201 agttcgaaca tccttcatac ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa
    34261 gatttgctgg tgttctttag aatattccgt taatatatct gttaatgcaa aaggggaaaa
    34321 gtcgtttttt tgataaatgt gctgatctag tcgaatcgca gggttcattc aaccggctcc
    34381 tttgtataaa ttcatatttt ctacaatatt atactctaaa atcaaagagt tgttttggaa
    34441 aaaaggtttt ctgtaaaaat ggacaagttg gttgacaaaa caaaagaaac taggaacaat
    34501 gtagctatga gaagtaaagg agaatgaata atggaattat cacaaggaac accgatttca
    34561 atcaaattgc gtacaaaagt aaaacagcaa ggggaagtgc aagattttta ttttgatttg
    34621 aaagggcaaa tggtgacaat tggcgatacg ctgtacatac gttataaaga gattcaggag
    34681 gagacgggcg atgagattcc tgtaacgatc aaattagttc cagatggtca tgtacagctg
    34741 atccgtgcag gagaaatgcg gatgcgtttg aaatttggct ataaagaacg gttagaaaca
    34801 tcctatcgaa caccatacgg tatgatccag attgctactt ttacgaaaga actgcatgtt
    34861 agtttgaaag accgtcccac agcagggaag gttcgaatcg attatgatct gttcatggga
    34921 ccggaaaaaa tcggcgaata ttatttgacc ttggatttta cagcgtaaat ataaaagaaa
    34981 agtttgattt tttgtgtctg attttgtatg ataaggagta gactgtgaaa ggacgtgtcc
    35041 tgattggaaa ttagtgtatt tgaaggtgca aacaaaagcg aattatcaat gatcgaagta
    35101 gcacacgcga tcttagaaca acgtggcgat gtcatggatt tttctgattt agctaaccag
    35161 atccaaaact atcttgggaa atcagacagt gaaattcgtg attcactcgc acaattttat
    35221 actgacttga atattgacgg cagctttatc tcattagggg ataatcgttg gggcttgcgt
    35281 tcatggtatg ctatcgattc gatcgatgaa gaagtaaacc atggaatgga cgaagaggat
    35341 gaagatactc cacgtcgccg taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa
    35401 gacgcgatcg attacaatga cgatgaccca gaagatacag atctgacaga agaagatgac
    35461 gatgatctgt ttgatgacga tgatgatgaa gatgaagaaa tcgctgctta taattctgac
    35521 ttgcaagaaa tcggagcaga tgacccgaca gatgatgaag aagacttgcc aggaaatatc
    35581 gaagaagatt taagtatcat agacgaagac gatgatgatg aagattatga agaggaagaa
    35641 ttttctgact ctgaaaaaga ataaacatta ttttcttacg agaggttgtg acagagacga
    35701 tcagcctatc agaataagga ggtgtgacac gatttttgtc acaccttctt ttttgtatat
    35761 aaaaatccca agtaaataca taaaaaataa aaaaattgaa acttttagcg caccttgtac
    35821 gtcatattaa gtgaggcaga aaggagtgga gaatgatttt tactggatat aagaaaaaac
    35881 ggcagatcga aaaataccgt cacctgctga atggactcat cgaaactgat tatcaaaaaa
    35941 tgttccgtat cacgatgaat tacgtacatg ataaagaaga ggccttagat gtgatgcaag
    36001 atagttttca taaagccctg aactattttg aaaaagaaaa agagatcgag cattttagtg
    36061 catggtttta tcggatattg atccgcacgg cattagatag ttggcgcaga caaaaaagga
    36121 cgcctgtgga cttatttgaa attcaagata tcccccaaca gttaccttta gaaacttccg
    36181 ttgcagagct ccatatgatt ttagataaaa tagacagtcc ggaaaaagag attctgatcc
    36241 tctacttttt tgaaggattt cgtttgaaag aaattgcgac tatcttggat atgaatgaaa
    36301 acacagttaa aacgaaaatg taccgctcat tgaatgcttt acgtcaaatc ctagaatgaa
    36361 ggagagaaga gtgccatgta ttcccaaaaa gaaaatttaa aaaaactaaa gaaaacttat
    36421 aaagaacaag caattcccaa agggcttaaa gaggagatcc gccagcgttt tattatcgaa
    36481 gagcaatcat ttataaaagc aaagcggaga aagagacgtc tccaagggtt cggagtatct
    36541 ttggctatcg taatgattac aacaagtagc cttcttttta atagtcaagt tcgaagcttt
    36601 gcagaagatt tacccatctt aggatcagtc attgaattga tcttaggcga acgctttact
    36661 gatcgatctg aaaaaatcga tattcaggta ccgaagatca atacacaaaa tgagaaagaa
    36721 aataaaacaa ttcatggatt aaatcaaaaa tactttcgcg aaggacaagc agattttgaa
    36781 aaagctgaaa aagaatatgg aaattttgaa acggatcatt accaagtttt gggagattat
    36841 cagaaaatcg tagacgataa ccggtttctc gtgattgaaa ggcagatcac ccaaacagca
    36901 gcagatagcc atgttgaaaa aagatacgac acaatcgaca agaaaaacag tgtccagctc
    36961 tcacttccac ttttattcaa agatgacacc tatctatctg ttttgacaaa agaagtcaaa
    37021 cggcagatgg ccgaacaagt caaggaagat ccatctaagt actattggac tgagcaagat
    37081 attcaagaag ggacgataga aaaaccaaca cttgttacgc ctacacgaag tttttatttg
    37141 aacaaagagc atcaattagt tttgactttt tctcaatacg aaattgctcc tggttatatg
    37201 ggtacaccag aatttgtcat tcctaagtcc gtgacaaaaa cgattttggc atcggaagat
    37261 tatttggatc attagacgag aagtagttgc gactagcagt ttcaattttc aagtaagtac
    37321 aagtaaataa taagaagaga aaaagagaaa gaagagatat agtgaaaaga aattatttag
    37381 ttatcagtat tattgcttta tccgtgcttt tgtttttcga aattacttcg gtggcctcct
    37441 tgatcaccga agtaaaagga caaacacact cttcggagca ttcgtcacaa tctttcaaac
    37501 aatcagaaca atcaaccatt aaaagccaat caactgaaac agctaacgtc aaagaagcca
    37561 ttcaagaatc agcggaaaag gtacaaagtc agtcatcaca atcaacacaa gacagtacaa
    37621 aaacgagccg agatccggct gtcaaaagcg tcgcaatcag ctttgatgat ggaccaggag
    37681 cgaccacaac accgcagctt ctgcgaatct taaaagaaaa aaacgtgcat gccacttttt
    37741 tcgtcttagg agaaaatacg gcgcagcatc ctgaaatcgt gaagcagaca gctgaagcag
    37801 ggcacgagat tggcaatcac acatacgatc atcagaatct ggccactctt tctgcccagt
    37861 cgatgacgga ggaagtgacg aaagcagaca ctgagatcaa aaaagtcact ggcaaaaccc
    37921 ccacttttgt tcgtccgccc tatggttctg tcacgaatgt aggagcaacg atcatccagc
    37981 gaccgatcat cgaatggtct gttgattcag aagattggaa gacaagaaat cctgatttga
    38041 ttttgcagaa gatccaagca acagtatacg atggggcaat tattttattc catgatatct
    38101 atcctgaaac gattcgtgcc gttcctcaag tgatcgatta tctgaaagag caagggtatc
    38161 gcatcacgac agttggggat ttattagggc atccaacggc tgtcgagaac tattatggca
    38221 gaaacgatca tcggccagtt cagtaagtga gacaagtatc tttaataaat aacacatttt
    38281 aataagtagt tgaacacata actaccttac ttgcaaaggc ttaacaatat ctatacaggt
    38341 acaacaaaaa actgtccacc aattttcttt ggcggacagt tttcacttaa aaataaagaa
    38401 gcaactattc gtaaattatg ctaaaatagc agtagaacgc gtcctgaggg aatcgaaccc
    38461 ccgtctcaag aaccggaatc ttacgtgata tccactacac taaggacgca agcactggga
    38521 taattgtaac taaacagata aaaaattaca aggggattgg acaaaaaaat gaaatttgag
    38581 caaaattttt cacaaaaaca gcagcagaca cagaaactag ccatgacgca acagttgcaa
    38641 cagtccatcc aagttttaca atataacagt gaggagctgt tggcattcgt tgagaatcaa
    38701 gcaatggaaa atccattggt ggaagttgtt gagccggaat ggcagccaga ttacataaaa
    38761 gcttcttcat cttcttatga aggagaagag acaaattacc taaatcagat tcctgataca
    38821 aaagggtctt tatttgattc attgatcgaa caagtccatt tgaattaccg agatactttt
    38881 ttgaggaaaa tcgttcttta tcttgtagaa tacatcgatt tgaacggatt tttgacgatt
    38941 agtttagaag aagccggaaa agagattgga gctacgccta tccaaatgct cgatgcgttg
    39001 actctgattc agcaattaga accagctgga gtaggtgcac gtagtttgca agaatgtctc
    39061 atgcttcaaa cagaacgaga tgattatgcg ccggaattag cttatatcgt gttggaagaa
    39121 tgttttgagg aactggtgga gcgaaaatgg aaagaaatcg cacaacgttt ttctgttgat
    39181 ctacatgctg ttcagcagat attcgactat ttacaaacgt tgagtccaag ccctggccgg
    39241 attttcgacc gtagcagcga attgtacatt attcctgatg tccgggtatt agtggacgac
    39301 gataaaaatg tacaagttat ctcaaaccga aaaaatcagc caaatatacg ctttcaagag
    39361 agttacttca aacaaatgtc acaacaagca gataaagaaa ctgaaaatta tttaaaagaa
    39421 cggaaacagg aattcgaatg gttgaagaaa acgatcctac aacgtgggga tacaattttg
    39481 cgggttgctc aagtcattgt ttcccggcaa aaagctttct ttatcgacaa ggaacgtcca
    39541 atcaagcctc ttacgttaaa ggaagttgca actgagatag atgtgcatga atccacggtc
    39601 agtcgtgcag tcaacgggaa atatttggaa acagacttcg gtgtatttga attaaaaaaa
    39661 tttttcacta cacgaattcc tacgaatagt acagaacaga cagaagacct ttcagcagat
    39721 acagcgaaga aaaagctgca ggaattagtc gatcaagaag acaaaaacaa gccgttgtct
    39781 gaccagaaat tggttgaatt gctgaaaaaa gatgagatag cgatctcaag acgaacagta
    39841 gcaaaatatc gtgatttatt aggaatcccg agttcatcaa aaagaaaacg ttatgataac
    39901 taatcagtca aaagactaga aaaagtattt cactttttct agtcttttta gttgaaaaac
    39961 gagagcaaaa ctgttagact tacagtcgtg aggttgagat ttccctttat tgatgtgaaa
    40021 ttcgaatagt taatttcttt ttttatcgta tatgggtcgt tttaagtcta cattggacgt
    40081 aaagtgtcca actaaggaga tcgctatgct ggatgaaata aaaatgattg aatctgttgc
    40141 accagatatt atcgaagtgt tgcaagaacg ctataaaatt ttacgaaata tctattggat
    40201 gcagccgatt ggacgtcgaa gtttatcgga aagtatgggg atcacagaac gagtattgcg
    40261 aacagaaaca gatcttttga aaaatctgaa tttgatcgac acctcaaaaa gcggtatgac
    40321 gctgacaact aagggcgaag aagtttatca aagccttgaa aacttcatgg atcagctttt
    40381 aggtacgcat caaaccgaac aacagttggc agaatatttt ggtaaccaac gctgtatcgt
    40441 tgtttcagga aacagcgaag agcagaccaa agtggctgat gcattcggcg aagcgctgag
    40501 cgaagcccta gaccgactac ttccagaagg cgagaatatc atcgctgtga tgggaggaac
    40561 tactatggct gtggtagccg aacaattgtc taatctggaa aataagaaac ggcataacct
    40621 gtttgtccct gcaagaggcg ggattggtga agcgataact gtacaggcaa attctgtcag
    40681 tgcaagaatg gctgctaaag caaacggtaa ccaccgtgca ctttatgttc cggaacaatt
    40741 aagtctggca acttacaatt ctttactcaa tgagccatct atccaagaag tcttaaactt
    40801 gattagtgaa gcgaattgtg ttattcatag tattgggcgt gcattacaca tggcagcacg
    40861 tcgtaagatg actgaaaaag aattagtcat gctgaaacaa gcaaatgctg ttgcggaatc
    40921 ttttggctac tttttcaatg aaaaaggtga agtcgtttat aaggtcccta gaatcggcat
    40981 agaattgcaa gatttggaaa aagtcccgat catcatggca atcgcgggcg gaaaatcaaa
    41041 agctaaagca attcgagctt atatgaaaaa tgcaccaaaa caaacgtggc tcatcactga
    41101 tgaagctgct gcaaatgaga ttttaaaagg ggtaaccctt taaaataaat atttttttga
    41161 ttttcataag gaggaaatct taaatgacag ttaaagtagg tattaatggt tttggacgta
    41221 ttggacgtct agcattccgt cgtatccaag atgtagatgg aatcgaagta gtagcaatca
    41281 acgacttgac agatgctaaa atgttggcac acttgttaaa atacgacaca actcaaggac
    41341 gtttcaacgg aacagttgaa gttcatgaag gttcattcaa cgtaaacgga aaagaagtta
    41401 aagtattagc taaccgcaac ccagaagaat taccatgggg cgaactaggc gtagatatcg
    41461 ttctagaatg tactggtttc ttcacttcta aatcagcagc tgaaaaacat ttaacagctg
    41521 gtgctaaacg cgttgttatc tctgctcctg gcggaaacga tgttccaaca atcgtttata
    41581 acacaaacca cgaaacatta actggtaaag aaacagttat ctcaggtgct tcatgtacta
    41641 ctaactgttt agctcctatg gctaaaacat tgaacgacaa atttggtgtt gttgaaggat
    41701 taatgacaac tatccacgct tacacaggtg accaaatgac tctagacgga cctcatccta
    41761 aaggtgactt ccgccgtgca cgcgctgctg ctgcaaacat cgttcctaac tcaacaggtg
    41821 ctgctaaagc tatcggttta gtaattcctg aattgaacgg taaattagac ggagctgctc
    41881 aacgtgttcc tgtaccaaca ggttcattaa cagaattagt aacagttctt gaaaaagaag
    41941 taactgttga cgaaatcaat gcagcaatga aagaagcttc aaacgaatct tacggataca
    42001 acacagacga aatcgtttct tcagatatcg ttggtatgac ttacggttca ttatttgatg
    42061 ctacacaaac taaagtaatg acagttggcg acaaacaatt agttaaaact gttgcttggt
    42121 acgacaacga aatgtcatac actgcacaat tagtacgtac tttagaatac ttcgctaact
    42181 tataagatag acaaacgtac aagtgtaaat tatagacatg tacatttaaa agcggggaag
    42241 caatcgcgct tcttccgctt tttttgataa taagtttatt caggaggaaa taaaatggct
    42301 aagaaaacag tgaaagatat cgatttaaaa gacaaaaaag ttcttgtccg tgttgacttc
    42361 aacgttcctt tgaaagacgg cgtgatcact gacgacactc gtatcaaagc agcattgcca
    42421 acaatcaact acgttctaga acaaggcgga aaagcaatcc ttttctctca ccttggacgt
    42481 gtaaaaacag aagaagataa agaaggaaaa tctttagctc cagttgcaaa acgtctaggc
    42541 gaattattag gtaaagaagt aacattcgta cctgaaactc gcggagaaca attagaagaa
    42601 gcaatccgca acatgaatga cggcgacgtt gtcgtattcg aaaatacacg atttgaagat
    42661 attgacggta aaaaagaaag caaaaatgat acagaactag gtaaatactg ggcttcatta
    42721 ggcgatgtat ttgttaacga tgctttcggt acagctcacc gtgcacatgc ttctaacgta
    42781 ggtatcgctt caactggtat cccaacagtt gctggattct tgatggaaaa agaaatcaag
    42841 ttcatcggtg aagcagtaga agaaccaaaa cgaccaatga ttgcaatcct tggtggcgca
    42901 aaagtttctg ataaaatcgg cgtaatcgaa aacttgattc ctaaagcaga taaaatcttg
    42961 atcggtggcg gaatgactta cacattctac gaagcaaaag gaatcaagat cggtaactct
    43021 ctagtagaag ctgataaagt ggaactagca aaagaattaa tcgaaaaagc aggcgacaaa
    43081 cttgtcttgc caatcgacaa tgtctgtgca ccagaatttt caaatgatgt tgaaacacaa
    43141 gtaatcgaag gagacattcc tgatggtttg atggctttag acatcggccc agcatcagta
    43201 aaattatttg ctgatacatt acaaggtgca aaaacagttg tttggaacgg acctatgggt
    43261 gtatttgaaa tgagtaactt tgctaaaggt acaatcggtg tttgtgaagc aatcgctaac
    43321 ttagaaggcg caacaacaat catcggtggc ggagactcag ctgcagcagc agaacaatta
    43381 ggatttgctg acaaattcac acacatctca acaggtggtg gcgcaagctt ggaattgctt
    43441 gaaggtaaag aattaccagg actagcagca atcaacgata aataaacgac caagtttgct
    43501 caattctgac gagtaatcga tcaaaaggtg aagggagttt tcatgactcc ctagccattt
    43561 ttcaataaat taaggagtgt gttcctcaat gcgtaaacct attatcgcag gtaactggaa
    43621 aatgaacaaa actgcatcag aagcaaaaga atttgctgaa gcagtaaaaa acaaaatccc
    43681 ttcaaatgac aaagttgatt cagtcatcgg atctcctgca ttattcttac aagaattaat
    43741 gacatcagct gaaggtacag aactaaaaat ctcagcacaa aactgctact gggaaaactc
    43801 aggtgcgttc actggtgaaa cttcaccagc agctcttgct gatctaggtg ttcattatgt
    43861 gatcatcgga cattcagaac gtcgtgaata tttccatgaa acagacgaag acatcaataa
    43921 aaaagcaaaa gctatctttg caaatggtat gacaccaatc ctatgctgcg gcgaatcttt
    43981 agaaacctat gaagcaggca aaactgctga atggatcgaa ggacaaatta cagctggttt
    44041 agctgatctt tcagctgaac aagtgtcaaa catggttatt gcttatgaac caatctgggc
    44101 aatcggtact ggtaaatcag ctgacgcaca aatcgctgac gaaatttgtg gagttgtacg
    44161 tcaaacagtt gaaaaacttt atggaaaaga agtatcagaa gcagttcgta tccaatacgg
    44221 cggttctgta aaaccagaaa atattgctga atacatggca aaagaaaatg ttgacggtgc
    44281 tttagtcggt ggcgcaagct tgcaagcaga ttcatttttg tcattgttag acgctgtaaa
    44341 ataattgttg aaaatttgcg aaaaatgcaa gaaatgtttg cactaatgag caaaattaac
    44401 taaaatcgta tatgagggaa atcccttagg aaaaaacaaa ctcaaaggag agacaaaaca
    44461 tgtcaattat tactgatgtt tacgctcgcg agatcttaga ctcacgtggt aacccaacaa
    44521 tcgaagtaga agtatacaca gaaagcggag cttttggccg cggaatggtt ccttcaggtg
    44581 cttcaactgg tgaatacgaa gcagttgaat tacgtgacgg agacaaagct cgttatggcg
    44641 gtaaaggtgt aactaaagct gtcgacaacg taaacaacat cattgctgaa gcaatcattg
    44701 gctacgatgt tcgtgaccaa atggctatcg acaaagctat gatcgcttta gacggaactc
    44761 ctaacaaagg taaattaggt gcgaacgcta ttttaggtgt ttctatcgct gttgcacgtg
    44821 cagctgctga ctacctagaa gtacctttat accactacct aggcggattc aacacaaaag
    44881 tattgccaac accaatgatg aacatcatca acggcggatc acatgctgat aactcaatcg
    44941 acttccaaga attcatgatc atgcctgttg gcgctccaac attcaaagaa gctctacgta
    45001 tgggtgctga agtattccac gcattagctt caatcttgaa agctcgcgga ttagcaactt
    45061 ctgttggtga cgaaggtgga tttgctccaa accttggttc aaacgaagaa ggttttgaag
    45121 taatcatcga agcaatcgaa aaagctggct atgtacctgg taaagacgtt gttcttgcta
    45181 tggatgctgc ttcttcagaa ttctacgaca aagaaaaagg tgtttacgta ctagctgact
    45241 caggcgaagg cgaaaaaaca actgacgaaa tgatcaaatt ctacgaagaa ttagtttcta
    45301 aatatccaat catctcaatc gaagatggat tagacgaaaa cgactgggac ggattcaaga
    45361 aattaactga cgtattaggc gacaaagttc aattagttgg tgacgacttg ttcgtaacaa
    45421 acactcaaaa attatctgaa ggtatcgaaa aaggaattgc taactcaatc cttatcaaag
    45481 tgaaccaaat cggtacatta acagaaactt tcgaagctat cgaaatggct aaagaagctg
    45541 gctacacagc agttgtatct caccgttctg gtgaaacaga agattcaaca atctctgata
    45601 tcgctgttgc aacaaatgct ggccaaatca aaactggttc tctatcacgt actgaccgta
    45661 ttgctaaata caaccaatta ctacgtatcg aagaccaact tggtgaagtt gcagaataca
    45721 aaggtttgaa atctttctac aacttaaaaa acaaataatt cttttctaat gaagatacat
    45781 gatcgactat tgaaagatag tcgagtatga tcacttagtt aagtaatcga actctctact
    45841 tgctgaaata tcagcaggta gagagt
//
LOCUS       DAZKOB010000011        45088 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000011 DAZKOB010000000
VERSION     DAZKOB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.011"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..670
                     /locus_tag="ACG8M6_000642"
     CDS             <1..670
                     /locus_tag="ACG8M6_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0456333.1"
                     /translation="KKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVDQ
                     INAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFKG
                     ANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSNDG
                     VYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVVQ
                     GELQ"
     gene            723..2699
                     /locus_tag="ACG8M6_000643"
     CDS             723..2699
                     /locus_tag="ACG8M6_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0456334.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            2712..3467
                     /locus_tag="ACG8M6_000644"
     CDS             2712..3467
                     /locus_tag="ACG8M6_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0456335.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            3464..4288
                     /locus_tag="ACG8M6_000645"
     CDS             3464..4288
                     /locus_tag="ACG8M6_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0456336.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            4298..4546
                     /locus_tag="ACG8M6_000646"
     CDS             4298..4546
                     /locus_tag="ACG8M6_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456337.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            4589..6688
                     /locus_tag="ACG8M6_000647"
     CDS             4589..6688
                     /locus_tag="ACG8M6_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0456338.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(6726..8783)
                     /locus_tag="ACG8M6_000648"
     CDS             complement(6726..8783)
                     /locus_tag="ACG8M6_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFQ0456339.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(8897..9109)
                     /locus_tag="ACG8M6_000649"
     CDS             complement(8897..9109)
                     /locus_tag="ACG8M6_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456340.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9296..9763
                     /locus_tag="ACG8M6_000650"
     CDS             9296..9763
                     /locus_tag="ACG8M6_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456341.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            9760..10002
                     /locus_tag="ACG8M6_000651"
     CDS             9760..10002
                     /locus_tag="ACG8M6_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0456342.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10020..10322
                     /locus_tag="ACG8M6_000652"
     CDS             10020..10322
                     /locus_tag="ACG8M6_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456343.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10394..12448
                     /locus_tag="ACG8M6_000653"
     CDS             10394..12448
                     /locus_tag="ACG8M6_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456344.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            12448..15060
                     /locus_tag="ACG8M6_000654"
     CDS             12448..15060
                     /locus_tag="ACG8M6_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0456345.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15057..17588
                     /locus_tag="ACG8M6_000655"
     CDS             15057..17588
                     /locus_tag="ACG8M6_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0456346.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            17640..17972
                     /locus_tag="ACG8M6_000656"
     CDS             17640..17972
                     /locus_tag="ACG8M6_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456347.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            17973..18566
                     /locus_tag="ACG8M6_000657"
     CDS             17973..18566
                     /locus_tag="ACG8M6_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456348.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            18729..20423
                     /locus_tag="ACG8M6_000658"
     CDS             18729..20423
                     /locus_tag="ACG8M6_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0456349.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21111..22988
                     /gene="ltrA"
                     /locus_tag="ACG8M6_000659"
     CDS             21111..22988
                     /gene="ltrA"
                     /locus_tag="ACG8M6_000659"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0456350.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23119..23496
                     /locus_tag="ACG8M6_000660"
                     /pseudo
     CDS             23119..23496
                     /locus_tag="ACG8M6_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            23496..24701
                     /locus_tag="ACG8M6_000661"
     CDS             23496..24701
                     /locus_tag="ACG8M6_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0456351.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            24717..25361
                     /locus_tag="ACG8M6_000662"
     CDS             24717..25361
                     /locus_tag="ACG8M6_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456352.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25372..26178
                     /locus_tag="ACG8M6_000663"
     CDS             25372..26178
                     /locus_tag="ACG8M6_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456353.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26200..26430
                     /locus_tag="ACG8M6_000664"
     CDS             26200..26430
                     /locus_tag="ACG8M6_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456354.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            26976..27251
                     /locus_tag="ACG8M6_000665"
     CDS             26976..27251
                     /locus_tag="ACG8M6_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456355.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27293..27715
                     /locus_tag="ACG8M6_000666"
     CDS             27293..27715
                     /locus_tag="ACG8M6_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456356.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            27734..29326
                     /gene="mobP2"
                     /locus_tag="ACG8M6_000667"
     CDS             27734..29326
                     /gene="mobP2"
                     /locus_tag="ACG8M6_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0456357.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29338..29673
                     /locus_tag="ACG8M6_000668"
     CDS             29338..29673
                     /locus_tag="ACG8M6_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456358.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            29820..30014
                     /locus_tag="ACG8M6_000669"
     CDS             29820..30014
                     /locus_tag="ACG8M6_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456359.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30046..32418
                     /locus_tag="ACG8M6_000670"
     CDS             30046..32418
                     /locus_tag="ACG8M6_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0456360.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            32479..33939
                     /locus_tag="ACG8M6_000671"
     CDS             32479..33939
                     /locus_tag="ACG8M6_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0456361.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            33950..36154
                     /gene="topB"
                     /locus_tag="ACG8M6_000672"
     CDS             33950..36154
                     /gene="topB"
                     /locus_tag="ACG8M6_000672"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0456362.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVGKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36170..36319
                     /locus_tag="ACG8M6_000673"
     CDS             36170..36319
                     /locus_tag="ACG8M6_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456363.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            36393..36986
                     /locus_tag="ACG8M6_000674"
     CDS             36393..36986
                     /locus_tag="ACG8M6_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0456364.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37002..37901
                     /locus_tag="ACG8M6_000675"
     CDS             37002..37901
                     /locus_tag="ACG8M6_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0456365.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            37904..38389
                     /gene="ssb"
                     /locus_tag="ACG8M6_000676"
     CDS             37904..38389
                     /gene="ssb"
                     /locus_tag="ACG8M6_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0456366.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            38755..39015
                     /locus_tag="ACG8M6_000677"
     CDS             38755..39015
                     /locus_tag="ACG8M6_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456367.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            39456..39662
                     /locus_tag="ACG8M6_000678"
     CDS             39456..39662
                     /locus_tag="ACG8M6_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0456368.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            39662..39913
                     /locus_tag="ACG8M6_000679"
     CDS             39662..39913
                     /locus_tag="ACG8M6_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456369.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            39928..40365
                     /locus_tag="ACG8M6_000680"
     CDS             39928..40365
                     /locus_tag="ACG8M6_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456370.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40358..41065
                     /locus_tag="ACG8M6_000681"
     CDS             40358..41065
                     /locus_tag="ACG8M6_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456371.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            41230..41430
                     /locus_tag="ACG8M6_000682"
     CDS             41230..41430
                     /locus_tag="ACG8M6_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456372.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            41446..41625
                     /locus_tag="ACG8M6_000683"
     CDS             41446..41625
                     /locus_tag="ACG8M6_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456373.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            41966..42229
                     /locus_tag="ACG8M6_000684"
     CDS             41966..42229
                     /locus_tag="ACG8M6_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0456374.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42223..42573
                     /locus_tag="ACG8M6_000685"
     CDS             42223..42573
                     /locus_tag="ACG8M6_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0456375.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(42597..43211)
                     /locus_tag="ACG8M6_000686"
     CDS             complement(42597..43211)
                     /locus_tag="ACG8M6_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0456376.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            43525..43947
                     /locus_tag="ACG8M6_000687"
     CDS             43525..43947
                     /locus_tag="ACG8M6_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456377.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            43957..44160
                     /locus_tag="ACG8M6_000688"
     CDS             43957..44160
                     /locus_tag="ACG8M6_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456378.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            44371..44955
                     /locus_tag="ACG8M6_000689"
     CDS             44371..44955
                     /locus_tag="ACG8M6_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0456379.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16507 a         7135 c         9134 g        12312 t
ORIGIN      
        1 gaaaaaatgg ttatttggtt ttttaggagt cactttaatc gtcttttgca gtgtgtttgg
       61 ctatgttagc taccaaaaac atgaaggaga ggtgtttaag caaaatatag aaaaaaagat
      121 gcccgtagat caaatcaatg cacatgcaaa gagttataag gaggatgcaa cgaacgtcaa
      181 taatgatatg tctttggggc aaatgttgtc catacaaaaa gaagcaatcg aaatgggcgt
      241 caataaacaa gtatttgcac aaattcagat tcctgcactt ggtttagcat tgccaatttt
      301 taaaggggca aatcaataca ctttgagtct tggagcggct acttattttt atgaagatgc
      361 cgaaatgggc aaaggaaact atgtcctagc aggtcataac atggaaatgc caggtgtttt
      421 attttctgat attcaaaagt tgtcattagg ggaggtgatg gacttagtta gtaacgatgg
      481 cgtctatcgt tacaaggtaa cgcgtaaatt tatcgtacct gggtacttta aattaatcga
      541 tggcgttcct gaagagaact ctttcttgtc tttgccaaag aaaggagaga aacccttatt
      601 aacgttgttt acttgtgttt atacgtcaca aggaaaagaa agatacgtgg tacagggaga
      661 gctacaataa caaggtttta atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa
      721 aaatggcaat aaatcggtat gtaaaaaaag ctggaatgct tgttggaatg ttaggaatca
      781 ttgcaggctg ctttcagggg tatcaagtac atgcagaaga tgtcacgcaa aaaacaccac
      841 cagagaaggt caatatcacg gttcataaat taatgtacga tcaaggaacg caattgaatg
      901 ttgatattga cgggatcaaa aacgatggtt atacgcacga tcaatatcct acaggtgtaa
      961 cgaaatacaa caaggcagat tatggtgatg ttgagtttac gttaggaaat attacggatc
     1021 aagtattacc gagagaagat tctgatctaa ccaacgcgaa agtggacgag attgtacaag
     1081 atgtagaaga caagggatct aacagtgagt acgtcaagaa tgcaaccaac aaaattactt
     1141 cagctgtaga tgaaaatggt gagatcactt ttactgatca accagcctat gtcaattcca
     1201 aagggaatgt atatgtggtt tatgaaagta aatccgctgc tggattagtc acacaaaaag
     1261 caaaaccaat ggttgtgatt gcgccaatga cagataaaac aggatcagga tttttaaaaa
     1321 atattcattt gtatcctaag aacattgttt ctaagttgag ttttgaatta acgaaatttg
     1381 gtgacgatgg cgcagaccaa agcaaacaaa cccttttaaa aggggcaaaa tttgagttat
     1441 acaagggcga acctggaaaa ggaacaaaat tgggtgatct tgtatcggat gatcaaggaa
     1501 agctgactgc gactgatttg acgttaggaa aatactattt tgtggaagtt ccttcggaag
     1561 ttgtagtggg atcagatcaa gaaccaacag cagatcaata tttattaggc gcagacgctc
     1621 gcaacgatgc gcacaataaa ttaacctttg aaattacgaa tgatggcgtg acaagtgact
     1681 taaaagcttc atacgtaaac tataaagcac cagtgatcga taaaacagta acaaatggaa
     1741 caggacaaga acattctttc caaattggtg acgcagtaaa ttatcaagga acgattcata
     1801 ttccaacaga cattgcaggt ggcgcagacg gtatcacagt aaacggtgtg aaaagcgaaa
     1861 cttctcctta cagtgtattt aaatggggag atacagcagg taaaggactt tcttatgttg
     1921 ctgaaaaagc agatattaaa gtaacaaata atgatggttc agtcgtatta aaagaaaata
     1981 ctgactataa aatcgaaaat tctgatagtg gttttgtgat cgattttatc gtaaataatg
     2041 gacaagtaag tgatacagtt gcgaatttac atggtcaaga tctaaaaatg acttataaca
     2101 tgtttgtgaa tgatagtgct gaaatcgcaa atccattaac aaatagcgtg gatttcgttt
     2161 ataacaataa tccatttaat caggaagaac accacgaaaa aacgaaagct gacgttgtga
     2221 cttatggcgc aaaattctta aaagtcgata gcggcctatt tggaacaggt atcaaagcaa
     2281 caccgcttga aggcgcagaa tttgcagcta agaatgcaga aggtaagtac tacggtggac
     2341 ttactgatac agataaagat ggtgtcaaag aagcagtttg ggtagacgat gtagcaaatg
     2401 cagcaatcct aaaatcagat aaagagggtc attttgaaat cactggttta actgaaggag
     2461 attactcact ggaagaaaca aaagctcctg aaaactatca aaaattaaca aaagagatct
     2521 ctttcaaagt agacaaagat tcatacaaag aagaaaaccg aatcacgatc aaaaataatc
     2581 aaaaagcttc cgtaccaatg actggtagca acggtttcca atcttacgta ttgatctctt
     2641 gcttgttgct aggagcaggg ggactttctg cagttgttta ctttaagaag aaagcctaga
     2701 aggatcagct aatgattatt cgtaagcccc caaaaagaag agtcatgcac aaactgatga
     2761 tatttttttg gttgctgtcg gcagtagcag tctatttaat tccagttgtg gaaacaaatg
     2821 tagcgaaatt tcaacaacag caaagaattt tagccattga aaatcttgaa aaaacagatt
     2881 cttctttgga tgttgcgaaa atagagttag gcgatcctgt tggtgttctt acaattccaa
     2941 gtatttcttt aaaactacca atttatgacg gaacttccga caaaatttta gagaatggtg
     3001 tagggattac ggaaggaact ggcgatatta caggaggaaa cggtaaaaat cctttaatcg
     3061 cggggcacag tggactttat aaagataatc ttttcgatga tttgccttca gtaaaaaaag
     3121 ggcaaaaatt ctttattaag gtaaatgaag aacaacatgc ctatcaaatt tatcgaattg
     3181 aagaagttaa agcagaagaa ttacaaaaaa attatttaga ttatcttatg cctgaaaaag
     3241 accaagatag gatcacgtta atgacatgca caccaaaagg tgtcaactca caccgctttt
     3301 tagtgtatgg tgaacgagtt tccttcagta aagaagattt aaaactacag aaaaaagcaa
     3361 aaaataggaa atcaatttca ttggattggc gattagcact gctattcgct tttttttgcg
     3421 ttcctattat ttttatttat aaaaaaataa ggaggagatc ggaatgaata agagaaaatg
     3481 gctgtttgtt tgtgtagcag ctctcttttc tactgtagct tattcgcaac tgtctttcgc
     3541 tgacagcagt gatagtagta ccactcaaac aatagataat tcaattactg caccaagcga
     3601 aacggacagt tcggagaata ctacagaaga aacggcagat tcaaatataa gctttactga
     3661 atcaacgaat ccaacgtcag aaagttcttc agagacttca gaaaagccgc aagacgtaga
     3721 agatacgcaa gtgatcaagc aagccattca tattcaaaaa attatttctt cgaatcaaat
     3781 caacaatcaa ggcagtcaag tagcccagca agaaggtatc aatggcgcga agtttattgt
     3841 ctacgatatc acgaatatct tagaagaaat gtcacaagat gataatgaga aaaatcaatc
     3901 tgtagaaata attgagtcaa aattgaaaga tcgagcaaaa aaactttcat acgaccagtt
     3961 gaaaaaagtg gctgaaggag aaaccaaaac gattgatgat caggaaggcg tgattgaatt
     4021 ttccattgaa gtacctgcaa acaaaaaaca agcatactat atcgttaatg aatcaagccc
     4081 tgaaaacatt tcaaacagtg aagatattgt tttattgaca cctgtttcaa atgaaaaagg
     4141 tgaatttttg acggatgttt ggttataccc taaatcagag aaatcagagc cgaaagaaga
     4201 aattaaaaaa gttgtgtcaa ctggtgtaaa gaagaacttt tttgaaaatt gttgggaatt
     4261 ttttgctcaa ttattttttg ataattagga tgaaatgatg aaaataataa gaatattagt
     4321 ttttaatcta tcctttttag ccgcaacaat attgtgttgc ggtttaattt tttctcaatt
     4381 atcaatttat ccaagatatc aacgattgtt cattcatttt ctaaacattc aatgtgcttc
     4441 catgccatta gaggtgatta aaaagctctt gatcaatcca atttttatgg ttagtttggt
     4501 tcttcttaca ggattggtaa cgtgggtatt aggaaaaaaa gaatgaaaag cttacgatcg
     4561 tttaaaaaaa tattagatgg agggcattat ggaaaataaa attcattgca ctacgggaag
     4621 agtaattact ttagtgttgt ttttgttcct tcttcttttt gcgaggggaa ctgcagtcca
     4681 ggccttagaa aaagttgatc cgagtcaaat caaagaaaat aataatctgg tgctttatca
     4741 tgatagtgat tcattgagaa atatcgaagt aactgggatc aatggattta agatactaga
     4801 tgagtattca actaaaaaac aaaaagtact tgcttggcca aaagtcaaag atttgaaaag
     4861 tacgtcaaaa atcgttgccc attatcaaaa agtaggtact tatctaggaa aagaaattga
     4921 ttgtgacgtg actttttcta attttgtagc gcacgctggg gttaaccaag acggattagc
     4981 taaaacaatt aatggcaaaa agtattataa ttatttgctc tttgataaaa atatttatcg
     5041 aggatttttc atgcagtcaa tcaatgtcat gaatgcaaaa ctagaattta agtatgcagg
     5101 aacaaaaaca aaagtagctt tgagtgaacc acagccagaa attcaatcaa aaatgagatc
     5161 gatgagagca gctggtattg atttaaacga tacatttcta tccatttaca gtttaaatgg
     5221 acagtatcag caacgtgtgt cttggtataa tgacaataat gaatctgtcg gtgaacgtaa
     5281 tattgatatg agagaatttg ttgggtatga aaaaatgggt agcttacctt attttgtcac
     5341 aacagataca gcatgtgcag aatacaaagc tcctgcgtta tcaacaaaca atttaacttc
     5401 aaaagtagtg ggaggacgtg cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt
     5461 tgtaggagct gatacttatc acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc
     5521 aacattcatg attggcgcaa atacgaatag tatgatgttt agctttgata ctgcattgct
     5581 atggacacca caaccatcga agcctacaaa agaagtgttt aacaaagcta atactgaaga
     5641 ggcagcacac aatattgaca aaaaagtgat tccacaagga tcagatgttt actatcatat
     5701 tcaccaaaag tttgatgcat taacagtcaa cacaatgaac aaatacaaat catttaaaat
     5761 cactgatacc tttgacagca aaaattttga tatggtatcg gatgggaaaa actatgatgg
     5821 cgcattgtgg tatgttgaag cgaatggtac ttggaaaaaa gctgatacag gaggaaagtt
     5881 aactgtatca ggtaatacag tcacttatac tgcttctgac agctttttag ctaacgctgc
     5941 aaattacggt agggattata ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg
     6001 aattgcacca aatgtggcta ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa
     6061 aacaaataca gtagaaaact atttctctct taaaccaaat aaaaaagtgg aacaagatgg
     6121 gaaagatgtt gatggtcgaa atgatggtaa aaaaggaacg cctacagctc ctttaaatgc
     6181 aggatctgaa gtacaatatc tagtcacaca aaaatggcat acaaaaggtg tagacattgc
     6241 ctcagatcat tacaagcaat ttagtattca agatccagtg gaagatcgtt tgacgtataa
     6301 agaaggttct gcgcgagtga tagatatgag tacaggaaaa gatattactt cagaaggtac
     6361 actaacctat gatagcaatt caagaacgct gaagtgggaa gcttccgctg atttcttaag
     6421 taaaaatctt ttagatggac gagaaattca gctgatattt acagctaaaa ctcctttgaa
     6481 tgaagaattg gatatcgaca accaagcaaa agtttcagta gaaagtattg agaaagcaac
     6541 taatgtagta acgattgggg taaaacctaa cttacctcag atcattgtgc caaaaactgg
     6601 atcgaatcat ttagtgctaa ctgttattgg aagtatatta tttcttactt tagcatcatt
     6661 gggctatgta gttttaaaac tcaaataaat ataaaaacaa ggctgagaat cagccttgtt
     6721 ttcttttatt tgaagattgg attcgcacta ataaatacat tatctacgcc aattctacca
     6781 gcagagtcaa ctacaaaatt atgacctaat ccgagaatac tttggttatt ataaagtaaa
     6841 tttgcattat gggcagggct atttttccat cctaccatgg catcgatagc accatcgtct
     6901 gataaaaggt catcaatata cagcccagtt tgacttatgt tttctccctt agcaccgtag
     6961 cagcctaagc tttccaataa atcttggaaa gtaccaccac cagggcgata gtgactgaag
     7021 ctgacttgta attctttagc tcgaatatct gcagcctgtt gaacaatatc attgaattgc
     7081 attggggcaa caccattttg ttggcgataa tcatttaacc aagcaagttc tttttgacgc
     7141 ataaagttac gtaattcgtc taatgaaatc tttttatatt tgaatgtgat tgttttattt
     7201 tgatcgacgt tattaatgga tcgttggccg tttttatcca cggtaacggt aggatcgtca
     7261 tttctgcaca agaaataatt ttgatcagtc atgtttttcc aaggagtagc aaagctatcc
     7321 ccttcgtata ctgtttttga ttcactagag agaacagcgt tggtatcagc atttacatga
     7381 ttaacagtca cattaaattt acgagtgtag ttgaaaatat aattttgact ttcagtcaca
     7441 ttgtttaagg ttactgaatt tgttggttct acccgatcgt ctaaaacata tccttgaata
     7501 gaaggagcag ttaaagtgac actgtcaccg tgagtgacat tgactatctg ttctttcaaa
     7561 atattaactc gtccttcgtt tttgtagttg ttattgcaca acaaattgat ttgatgcact
     7621 tgattataat tgaatgtgat tgttgtatta cctgtgattg ttttgctttg agtatcatta
     7681 ccaatcactt tgtagctaaa attatcttta gcgccttcgc tatatttaaa tgcttttgcc
     7741 ttagcagtgt aagtagaacc agaagttact tttgcttgat ctgtagcaat cacttgacct
     7801 gtttgattgt caatatggtt aatagtaacc gtatattctt ttggtgtttc aggtttgtgt
     7861 tgcaaccctt taaatgcagc atcaagacta tttttagcat tgtttacttg tgtttgagaa
     7921 accttagaat cgtctagtac ttttttagca ttggataaag cagtctgaaa attattccaa
     7981 gattcttctg taaaatctcc tttagcagtt gattttactc ggttgtataa agcttgtaat
     8041 tcagctttat tcacagtagg aactggtttt ttctttaatc ctttgtaagc agaatctaat
     8101 ttatttttgg cactatccac ttgcttttga gtcgcttttg agtcatttaa cactttctta
     8161 gcattgttta aagcggtttg gaatgcgttc caggtattgt cggtatagtc accttttttc
     8221 gttcccttaa cgctcgtata aagagcttct agagccgatt tattcacttt gggtttagag
     8281 gtgtcagcgc cataccaacc gataccttcg tatttccagc catagtcttt ggttaaccat
     8341 tgtacttctt tccaacttgc agtgtagtga tgtgagccag attttacacc aggaaggaaa
     8401 aggcggtaga tcggtttatt atttttttcg gcagaacgcc aggcaggccc ttcataagtc
     8461 cagccgtaat ttttagtcaa ccatttcact tcatcccaac tagctgtgta atggtgatca
     8521 cgaagacctt tattgtacaa acgatagaca agatggccac cgctagtcgg tgcttcccag
     8581 gcggggcctt cataatttcc ccattttatc tttactagat ggtcacgttc ttttttgcta
     8641 gaggtgtaaa aatgttcatt attatttgga ttgtaaaggc gatacatgac gttagttgaa
     8701 gcagcgtcaa cagatccagc aagactaagt gaggcagcaa atgttccaat agctaaaaca
     8761 gtttttggat taaataattt cattttttca actcctaaat ttaattaaaa atgtttacat
     8821 gattattata tatcggctaa ccgatatagt caataaaaaa taagaaaagc cttatataat
     8881 tttggctttt cttattttat ttgttagttg aagtattaat atcaacgatt tcttcagcca
     8941 atttatttaa tttcatagca tttatgaatc tcatgttgtc tatactagag cgtttgttgg
     9001 caaggtcact tactgtagtt aagggtacgc cagattctat ggatatagaa cgaactgatt
     9061 taccactatc aaacagcaac cattcgattt tctttttatc agcgtacatt tcaatgctcc
     9121 ttcttgtttt ttctttatta tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa
     9181 ttttctatag aaaggtggat ttttatgagt attcgttctc cgtgtatcca aatgaggttt
     9241 gttgctactt tcgttatttg atatactaaa aataaacaac gaaagaagga ataaaatgaa
     9301 gaaaaagcaa ctatggtttg tctctatgtt tgcaatattt tttattattt taagtggatg
     9361 ttcaagtaat aagaaatcgg aagctaaaga agataatagt ttattgcaac caggaactgt
     9421 ttggaaagaa gaagtaggtg gtcttattca taatttaaaa attatagatg atactacctg
     9481 ggaatattct gaaagtgtat ggcatccaga tcctgtacaa ataacagtta agaggcaaaa
     9541 agactatgaa ggtttagaaa gatacaaaat agtagattct gcaggcatta gggaatttat
     9601 aaacaaacct gattcattat ttattgttgt tccctatgag aaggatggcg taaaaaaaat
     9661 aatttttctt ggatctagca aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg
     9721 atcagagtta aataaataca aattacaaaa gactagtgag tgattgctag tctttttatt
     9781 tatcctgaag gaggaatcac agtggcaaaa gttaaaacac cattcgaaaa agccgcgtct
     9841 aaaatcttag aacgccgtgg tgtttctgaa atacagtgga aaaaagaagt tcttgaaaaa
     9901 gcacaatttg acctattacg aggaaaagat aaggacttag aagaatatat ctatcaacgt
     9961 gaacgtgaaa aacttgtatt cgatgaaatt attaaacatt aaaaggagcg aataatctta
    10021 tgtttgaatc aattacaatg aattttttaa ctcaaccgaa agtagtagct gcagttgata
    10081 tttttagagc attacaaact gccgcaacta gtttgaaaaa taatctgatg atttttgcgg
    10141 ctgtagctgc cgttgcctcc attgctcttg gaggtatttt cttcttcttt gggcgaagag
    10201 gagctgaaaa cgggaagaca cacgtaggtt atgtattgtt gggggtagga gttattttag
    10261 ctgccgcttc tattgtagtt tcaatgttct caatgtttgg acaaacccca caaggactat
    10321 aaaaaaaaca agtaggagga gtaaaaagta cgtggctact ttttactcct ttttttatga
    10381 aaggagaata ttcatgtttt ttacgatttc aggaaatgca aaattggata aagagttaat
    10441 aagaaaagca acgaagaaag tgggaaagcg tacttccttt ttaccgaaaa caattaaata
    10501 ttctgtcatt gaaacggaag aacagctaca tgtgttagga caagtgttag aacaagagaa
    10561 tcaatcttct gttaaattga ctctcacgtt acatgataaa gaaggaaaaa tagaaaaggc
    10621 tggcacagaa tacgtttttg gcccactgga ttatcaaatt tcagaaggtt tttatcctac
    10681 attgatagat ttaatccgcc ttgatgtgtt taaagatacg gatgaaacta tgagtgtgga
    10741 agagtcttat cgaaacgaaa aactgctaca agttttagaa gaagagttag agagcgacca
    10801 aagaaaagtg aatcaagtga gtgaagtcga agtaaatgaa gaagtaccat gggaacgacc
    10861 aatctttcca aagaaggagt caaaagaaaa agagacagtc tatgttgacg aacaagctga
    10921 tattccgact cagaaatcga tatttgagga agagaacgat gtgatgggtc aattggaggt
    10981 agatcccttt gaagaaacaa tggaggatga agagacggac gaagtagaag ttgatccagt
    11041 tgaagctaat gaagagtcaa catggactga aggggatttt caggaatata ttgaaccact
    11101 tatcagtgaa gaagtagaaa aaaacgaaac agagatacaa aaggcaactg tatttgatta
    11161 tattttggat caatatccgc cagagcatga atggatgaaa gaaaaaaaca aattattttt
    11221 aaatgagctt tattcgaaat atcaattacc ccaaacctat caagcatttt tagagagtaa
    11281 agaagaatta atctcacagg gtaaaggcac acttttagaa cagttagagt ctgtccaatc
    11341 aacaaattgg catgagcttg cacaagaaga tttattagcc acctttgaag aacgtcaaca
    11401 agaaacagat caagaaatta atgagtatgc gacggaacag tcgtctaatt gggagaaagt
    11461 aaaagaggaa ctaaataaag aagaacagcg agtgattgaa gaagaagtac aaaaagtcaa
    11521 agagcggtac gaaaagaaac gcgacaatgc ttattccttg tgtgtagaaa agatcaacaa
    11581 ttttactaag acgaatcaag aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg
    11641 tattaaagaa cgaaaagaag agttctaccg cggtttacgt acagataagc taaagattag
    11701 caaagaatta aatgaacaac tgaataagct atatcaaaca acgactgaag ttcttatgga
    11761 aaaacacgaa cagatccagc aagaattaca agtacaaatt ccaaaatgga aaaaagagta
    11821 tgaaattcaa caacaagaat tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga
    11881 gcgtgaacaa ttagaaataa gaaaacggga acaggatctt gcggaacaaa aaatggcgat
    11941 agaaaaacaa cggttagagc aagaaaagga aaaagaagca gctgctcgta aggaacgtca
    12001 agaacaaatg gatacattgc gtcaagcaca gttgaattat ctagcacaac ctcaaccagt
    12061 agctgctgtt caaacgccag tgaacaacca agcaaattta actccttcac cccagcgttc
    12121 ctttaacgga tggatggtgg gatgtattgc aagtttaagc ttgcttctag gtggaggaac
    12181 agtatttgct tttaatcata tcaatcaatc ttcacaggct gattctgtag ccacaacggc
    12241 gtcaatcaag gaagaagccc aggaacaagc aagaaaagag tatgaacaac aattagaaga
    12301 attattgaat cgtaccaatc agtcaacaac agagtcaagc atggaaaata gcaaagagag
    12361 tgatcctagt aaggctaaag aaggatcaag cgattcaact gtacactcaa acacttccga
    12421 aagttcagaa tcaaatgagg tgaaataatg cgtattagag aaacaacgag acgaaaagac
    12481 aaacgattca atttagaaaa gaataggtat ctttctcatt tctatcgcta tactccttca
    12541 ggagaagagc taccagaaaa acatgacaaa cgtatttctc aaaagatttt ttatagtggc
    12601 ttagcttatc ttcctttaaa ctatgttgtt acaagaatac cagaagcatt tcatcgattt
    12661 gagattattc caggaagttt acaaggagtg aatatttttc agttacaaga aatgccgcag
    12721 ttattccatt ttgattcaac tggtttttgg aatcctttga ccccaacaac aagtttaggc
    12781 acacgtttag ttgtttctac gatttgtgtt gttccagctg tacttatggg attgaaaatg
    12841 gacatgtggt ttcgtccgct agcagatgga caaaaagggg ataatcgatt aatgacctta
    12901 aaagaaatcc aacagattta tccgcaaata cctgaaacaa aaactacttt tccaggttat
    12961 ggaggtattc caatcacaca ttacaaaaaa tattggtaca ttcaaacaga tactgttaat
    13021 acgtgtattg ttggtacctc tcgatcaggg aaaggtcaga ttgaagtgtt agccaccatt
    13081 gacaatttat ctcgtgcaga aaaacaaagt tcaatggtcg ttaatgatcc aaaaacagaa
    13141 ttatatgttg gtagtaaaga tgaactagaa gctagagggt atgatgtata tgcctttaat
    13201 atcaccgatc cactgcaatc aatgagtgat aatccattgg cactcatcgt gaaatactgg
    13261 aaacgaggag acatagatac tgcaacacag ttaaccaata ctttcacaaa tacgatttat
    13321 tatgatgcca acgcttcaga taacaaatac tttaacgaaa acgcgcagaa agctgtcaat
    13381 gcgcttattt ttgcgttgtt agaacaagct gatcaaacag gagattactc caaattgaca
    13441 ccgaataatc tcgttgagct tctttctgag attggcggtt ttaattatta tccagatcca
    13501 gacaatgaat tcaaacagtt gaatgcctta gatgaattta tgaatcagtt gccacctgga
    13561 aacattgcaa aaaaacaata tggggcaacg aaaattggat ctgataaagc gaagggaaat
    13621 attttatcaa cggcaatcac gggattgaat ccatttactc taacgaaaat tgcaaagatg
    13681 acttctcaat caacgattga ttataaatcg gttgggtttc caaaatactt agatttgaaa
    13741 ttacatgaaa gtcttttaaa tcaacgagtc gtagtagaat tttatcataa gggaaagaaa
    13801 tttcatgaag aacgtgtcaa agtaggctat aaaggattta gtgaaatcaa ttttgattgt
    13861 catttagttg caggtgatca agtttgttta aaatacgatc atcaaggaaa agaatatgtg
    13921 gcttggtatg aattttctca aagagtattg aaagacgaag agggtaatat cgtttatcgg
    13981 aaaaaacgag gagaaaccca tttgccagaa ttagaaaaag aagccgttct aacattagat
    14041 gaggaaaaaa gtaacttatt agtagagtct attcggatgc actataccga taaaccaatg
    14101 gctattttta tgcttacacc tgattatgat ccatctaacc atatcattgt tagtattttt
    14161 cttagtcagt tatataaaga attatctacg caatgtgtta agactaaagg agataaatgt
    14221 caccgcagga ttcattttat tttagatgaa tttgggaata tgccaccaat ggataatatg
    14281 gaaggaatca tgacggtcac agctggccga aacatgttgt gggatctgtt tattcaatct
    14341 tatcagcaac tttatgcgaa gtatggagaa caaaacgcag cgattatcaa agaaaattgt
    14401 cagacacata tttatattat gtcgacagat gataatacca tcgaagaatt ttctaaaaaa
    14461 gtggggaata agacggttga acaagagaac agtaccatca attcattagg tatgaatact
    14521 catatcaatc gcaatgttga ttcagatcgt atcttaactc ctgaaagaat ttctactttt
    14581 ttagaaggag aaacgattgt actcgctcct ttaaaacgac aagatttgaa aggtcataaa
    14641 atacggccgt atcctatttt taatcatggg gaaacgaatc ttccttatgc ttatcaatat
    14701 attgaagatt tcaatccatc gtgggatatc aatgatatag acatacattg tacacatgcg
    14761 gacttggatc ttaaggcttt acaaattgat tatgattatt tcctaccaga tgaatatgct
    14821 agaaatctct atcatggact gcatgatcaa ttagaagaaa aaaacgaaga aaatatgttt
    14881 tataaaatcg ttcaatcttt tgagaatgag acggctaaaa aattgttaat tcaattttac
    14941 gaagaacaag cagcagatcg acttcaagaa tatgttgaaa agttgatcaa aaataaagaa
    15001 atttctgaaa tgattgggaa acggttgtta ttggaatctg aaaaggtggt gaaagaatga
    15061 tcattggagg aattttttct ggcatagccg actggtggtc aggcttattc ggagaagggg
    15121 atgcaaaccc caaaaccgta acgaaagtct tacaagatta tgggaattat ctgcagtatc
    15181 aagatgtatt tagttggatt tggaacactt ttctgtgggg attgattaaa ggattatata
    15241 atctcaatac tttactagaa aaaaccattt atcaaagctt tgatttaaaa gacttattga
    15301 acgccgctgg ggtaaatgca ctatttcaaa gtttgattag taaggtattg gctctttttt
    15361 gtgtcgtcac attaatttat ttaggcttga aattttttat gagtaaacac ccaccaaaag
    15421 tcaaaaatat cttggttaat ttatttatgg ccatgatctt gatttttggt ggtagttctt
    15481 taatcgatga agggttaaat atcagtaaaa gcttttacgg agacgtcact gcagcaaata
    15541 aagcagataa aacgggatca cctgcttttc aaattattca aaataatgtg atcgatgtca
    15601 gtacgttact agcaactgac ccgaataaag tagaatccct accaacagat aaaagaaatg
    15661 ctttaacagc gaaaaatttt aaaacagcga atatcaatgg gattattact cctgaacaag
    15721 ctaaaggtat ggcaggtaat gataaaagtg ggttatcaga tatacaaaag gatcggttaa
    15781 aggcattaca ataccgattg gaactagatg ataatgggaa agaaattcct gttcaaatta
    15841 gtgacgaagg cttagcaaaa tatgtctata cttcagggta tcgtcgatac agtagtaaac
    15901 caggtattat tttagctggt gaatcttctt tggcagtagc ctatttattt atcctattta
    15961 cgatcgttac ttgcattatt gagctggtat ttaaaaaatt ctatttagtg attgccgcgt
    16021 caacggactt tgagacaggt caacggatga aaagtgcgat agaagatatt tctcaatcct
    16081 tcttgttatt ggcgtttacg attttagaat tacgaatcta taccttaatg ctttcaggta
    16141 tcggtgattt gcatgcggca ggaaagatca atggattctt gtatgtcgtt gctttgattg
    16201 tactaaccat tgcgctattc aaaggctctc aatcagttac gaaaattttc ggcgttgata
    16261 caagcctgaa aaacggcagt aattctcttc ttagcatgtt tgccttagga aatattgcga
    16321 aaaatgtggg tagcggagct aaaaaccttg caagtgctgg taagagtggt ttaagccatt
    16381 taaatgatat tcgtaaaaat ggatttggcc atcgaaaaga tgttacccct gataacgggg
    16441 aagaacaatc agacgataaa gctacaactg tacctaatag taagaaaaca gtttccgaaa
    16501 tgttcgggaa agcgaaaaat ggtgtttcta aagccgcaga aggtcttggt tacgtcaatg
    16561 aacgtgggtt aaaaggaaca gctgaagatc ttacagaaaa agccaaagac accttagaag
    16621 ataaattaga cggcacgaga gctaaagaat tggctgaagc tgtgaagaat ccatccgaag
    16681 cactagaaaa acaaaaagaa aaagcttcag aagtcaaaga aggactaaaa gatgcgtatg
    16741 gtgaaggaca agtcaatgca gcagtgaaag ctaatacaac aaccgaaaaa gaaaatcaag
    16801 aaggtatagt gattccttca gtcaaagatc accaagaagc gttgcactct aatgaaaatt
    16861 cgattgaaga gaaaaattcc atagaagaaa gaaattcaga agaaggaaaa aatggacagt
    16921 taccaaaaac tgatccagaa caatatcgct ataaacctaa cagtgacctt gaaaatgaga
    16981 agcgaatttc agatttatca aaagtaaatc aagaagacaa agcagaaaat gagggacagt
    17041 taccaaaaac tgatccagaa caatatcggt atagatctgt taactccgaa aatgaaaaac
    17101 gcatttcaga tttaccaaaa cgaaatcaag aagtacaaac ggatgaggat tccatcgttc
    17161 tgccaaatga taatggaatc gaagttggaa ctgaaaaatc aacgaaaatt ggtttaccta
    17221 aggcacaaaa tttaccaatg aaagatagtc gtttgaatca attcaaggat aatgatcaaa
    17281 gttttgttca tcaatcacct gcaggtactg ttcaaatgaa accaagtcgt ttgcctgaaa
    17341 atggaaatct gacaactgtt tcttacaatt tatctgaaag tggtgagaca gggaatgcgt
    17401 ccgttcaaaa tgttgtgaca agagaagctg caccaaaacc tattcaaaac gttcatacag
    17461 gttacaagcc agcaaatgtg gcatttactg caccaacaaa aataaatgtg acaagagaaa
    17521 caaaaaacag gaacaatgca ttgaaagaac aactattgag ggatatcaaa caggatatca
    17581 aaccataaaa ggcctacaag ttaggccttt tttctatgta tgaaaaggag aaatcattta
    17641 tggataaata tagtaacatg aaaaaccctc caagatttag tggagaatgg aagtttttcg
    17701 cttttttctt tttagatcta ttaacgcttg gtggctgctt tttttgggcg aaaatggtca
    17761 ataatcttgt ccacttacca ctcttttttg cactcttgaa ttatggctta tggattgcga
    17821 tagcgttagt gattgtttgg cgaccctata ataacccagg tcaacgacaa atttttgcga
    17881 ttatcgagtc tatttttttt gcggacaaga accattatca tgcgttagat cctaaccgaa
    17941 attatcatgg tacgtataag gaggatgaat aaatgcgagt caaaggacaa aattcagttg
    18001 aaaggaaatc cattttgcag tttggaaaac cagaagagcg ctctatcttg gacttatttc
    18061 catataaatc ctgtacagaa gagggagtct taattactag agaagatcat tttcaacgtt
    18121 attatcgtgt agtttctaca gacgtggaag gattaaatga acaagagaaa ctagaacgaa
    18181 tgaatcagtt gaccaccgtc atgcgtacgt atgtgccaac aattaagtta gttagtttga
    18241 ccacagagac ggatctaacg gaacaaatcg tgcaaaagcg gcaattatta gataaaaatc
    18301 gtatggaaca gtccttagga agaaatctac aacgattaaa aaaatatgaa aagaaattag
    18361 tcgaagaaat ccaggaattg aaacgagcag aacaagaacg tcctgactta agcttctttt
    18421 tcgtcattga aggaaagaca atcaaggatt tacaaaccag aaaccgtcag ttaatgcgat
    18481 ctagtggcat tttagggtta aaaccactct acaaaaaaga attgatcaag attctgtatc
    18541 gaatgaacaa tatgaatgat gagtaggaat ggagaagaga gaatgaaata tccgttgata
    18601 tggttagaaa aagatgttcc gaaggaagag accactagaa aatttcctca cactttaagc
    18661 ctgggtaagt tgggaaaagg gcaatctttt aaccaagtaa agaatcaaac aagaagaggt
    18721 gactgtacat gttagaagca attaaaacag ctttttcacc acaaaatgat caaaaagtac
    18781 ttgatccatt aaagcaacta ggaaatcggg aaatagaaat tgtaaaatcc aaagggtatg
    18841 atttagattt tttaaaacaa gtgatgccac aaggtgggtt aaattttgat gaagaatatg
    18901 tgacgtatgg taatggttat tcacgatgtg tgacagttta tcactacaca aaaaatccta
    18961 cgcttttttg gttggccaat ctcatgaaca atcctcatac agtggccaca ctcgatgtta
    19021 gcacagatga aaagcaaaaa gtgattgatc aaatcaatcg tgccttagat gaattaaatg
    19081 atcgatcaga taatgaacga aaagcgactg accgaaataa aagcgcggaa gaatacctgg
    19141 aactagcgga atatgctaga aaattgactt ctaatgggga aatttctaag caagtcaaag
    19201 tgagagtaca tgtttacgga aacacgattc aagaagtaga tgaacgagcg caggaactga
    19261 tcgaaaatct caaagggatg gattatcgtg cggtgactta tctgcataaa accaaacgag
    19321 attggcaatc gatgtttacc atgttcgatg aacaagctga atggttcgga aatatcaaag
    19381 ggcaatcact accatcgtta aacattggcg gaggtttccc gttccaccac caaacgctaa
    19441 aagatccagg agggacacaa tatggggaaa ccatgacagg cggcccattt gtctggaatg
    19501 cgacgtataa aaatagtgta cggttgtcta ctaatacctt gcttctagga atgatgggag
    19561 ctggtaaatc gaccgttctt aaaatgatcg cggaagccca tttagctgca ggagacttta
    19621 tttggggctt tgaaaaggga aaagacttta ttcctttctt aaaggaatat aacggtatta
    19681 tggtgcgttt agacggatct gacggaatga tcaatccttt agagatattt gccacacgga
    19741 cgtatgatga agcatccagt ttatatgacg atggatcagt caaagatttg aagatcaatg
    19801 aagcagcgtc ctatcaaaca cacttagaca aagtggtcta tcaggtacag ttagtgagtc
    19861 ctcagctaaa aggaacgatg aaagcagaat ttaagaccta cttaaatcgt ttttatgaag
    19921 agtatggaac agtaccacgc ggctttactt cctcaaattc gcgttcaaat actgatacgc
    19981 aagtcacagg gaaagatcca gaagcttatc cgacgttcaa agaattttta gactttttag
    20041 gacaactgga attaccaggt gcttcccaag agaaaaagaa tcgaaaagaa gaaatggaat
    20101 ccatcgtaga atccttatgt gagacgtatg gcatgatttt tgatggtcat agtacgattc
    20161 gacaccttga ccaacaacag ttggtctttt ttgacattga ctctattaca ggcttagatc
    20221 gaggggtaca acaatgtcaa atgtacatgg ctatgacgtt gatttggaat caggcattga
    20281 ttcaaggaag aagacaacga cgtttaatta aagaaaaaca aatggaagaa caagacttca
    20341 aacgcttctt ggcgatgatt gatgagtgcc ataatggtgc gacctgttct gcagtgaaaa
    20401 gctgtgggtt gcaagtagtc taaaaactat tgtaataact cttataacga gaagtcaaat
    20461 gagtgggtaa cgccacaaag ggcttccact gatactccga catgcaacaa gtagtttatg
    20521 cagaccaacg ttgtgtgaag ctcggtgaag tcggctgaag gataccgtaa ggtaaaagaa
    20581 atagtgaata ccacgaaagt tctctagagg taaagggtgt atccgataag tcgggtgtcc
    20641 aaaaaaactt aatatggtga gtatgtacaa acgtactgac gaactagcga atgtactgtc
    20701 cgaaaggcat agctcctaga aatgggagag acagtgagca aaaaatcact cattgtgcta
    20761 acttgtggtt gagtaaaagt cgttgttatg aaagaccatg ctgcgttaca ggcgtaggag
    20821 aagttagggg gcaaaagcta gcatctaagt taaggcatgt ggatagatat aagggaacag
    20881 gggaagccat caacatggag agtgcatact cgatgaagtg gtagtaagaa atatgtgaat
    20941 gacaaataac ttactttagt gatggtgaaa gtagagtcac gactgatgaa ggttctgtaa
    21001 tggaactgga ggaacaggct cgattcgtct gtaaaagtca tgaaacccgt tcaaccatgg
    21061 gattctcgta tgactaaaaa ggtcacgagc caattggagc gtgataaacc atgacaaaag
    21121 aaacaagtcc aaaagatagg aagttgcggc atgcagaata ttatggcatg acggaaatct
    21181 tcgataagat atactccaaa agtttgaagg gagataattt caagaatcta atgcagatta
    21241 tcatttctga taacaacatt gtcctagcat atcgtaatat caagagaaat cacggtagtg
    21301 tgacaagagg agaagatgga gtaacgatta aagatattga aaatctcaac cgtaatgaat
    21361 tcgtagaaat tgttaggaag cgattcgcac attataaccc tcggaaagta aggcgtgtag
    21421 aaattcctaa atataatggt aaattaagac cgttgggtat tccatcaata tgggatagaa
    21481 tcgcgcagca atgcatctta caagtattag aaccaatatg tgaagcaaaa ttcaataatc
    21541 acagctatgg tttccgccca aatagatcag cagaacacgc aatagcagat tgtatggccc
    21601 gaatgaatca aagtcacatg gaatatgtgg tagatgtaga tatcaaaggt ttcttcgacg
    21661 aagtcaatca tactaaactc atgagacaaa tctggtctat gggtatacga gacaaacagc
    21721 ttctcttaat cattaggaaa atgttaagag cacccgtagt acttccaaat gggaagttga
    21781 tatacccaac aaaaggaaca ccacaaggcg gaatacttag cccattactt gcgaacatca
    21841 atctcaacga atttgactgg tggatagcaa atcagtggga ggaaaaagat gccaaagagc
    21901 taaaacatca ttttaggaaa aatggagctt gggataaagg taatatctat cacaagctca
    21961 gaaagagtac aactctaaaa gaaatatacc tagtgagata cgcagatgat tttaaaattt
    22021 tctgtagaaa ttataatgaa gcgaaaaaag ttttctatgc aactaaaatg tggttacagg
    22081 agcgtctaaa gttaccaata tctgaagaaa agtcaaaaat cacaaatctt tcaaaagaag
    22141 aaagtgaatt tttaggcttt acgatgaaat tagtgaccaa aggtaacaaa aaggttgtta
    22201 atacgcatat aggtacaaaa gcacaaagca tgattaaagt gaaactaaaa aaacaaatca
    22261 aacgaattca aaaaagtcct aatagcaaag atacctttat ctatattgga gtctataaca
    22321 gtatggttat tgggattcat aattacttca aaattgcgac tcattgtagt gtagcaatgc
    22381 gaaaaattgc aagagaactc agaccaagtt ttgaaatacg actacgagaa caaggaatct
    22441 caaaagaagg acaatacgga aagtacgaag gatacaagcc ttacctacgg tcaggacaaa
    22501 tacgatatat aagagggttt ccaatattac cagtagggta tattcaacac agacatccga
    22561 tgtgtaaaaa aagaaatatt aataaataca ccgaagaagg tagggaagcc attcataaga
    22621 agcaacaaac cgtagaagaa tggaagatat cgtggttgtg tagcaatcca gtgcttagta
    22681 agagagcaac gattgaattt gcggacaatc gaatttcaaa attcatcgct caaaagggaa
    22741 gatgtgcagt aacaggtgag gagcttatct tatctgaaat gcattgtcat catattatcc
    22801 cttatcatga aagcaaaagt gatagctatg aaaacctagt gattgttact gaggaggttc
    22861 atcgggtaat acacgctaca caaagtgaaa cgattgagga acttctaaaa tatctaaaat
    22921 taaatccaaa acaaaaagaa aaactcaatg agttgagatt aaaagttggt aacgaagaaa
    22981 tatcttaata aatatttgta ggacgggaaa acagaatgtc ttttatagac gagggaacgc
    23041 cgtatgcggt gaaagtcgca cgtacggtgt ggactggggg aaaagacgga gataatatca
    23101 aactcttacc tatcagtatc gattaatcca gattttattg aaacggtaaa ttatgtcaca
    23161 aactttcaac gagaaatgcg taaagtagaa gcaatgactg ttttagctac acaatcccca
    23221 caagaaatgg tgccagaaaa tatgtcttct gataacttct cgaaactgaa gaaagtcttt
    23281 gaattttctt ctacgaagat ctttatgcgt atggatgaat ctattttacg tcatatagaa
    23341 ggattagtgg gtaaatcaat gacgaactca gaattagaat ccattcctca acaaaatcaa
    23401 ggagacgctg tgatcaattt tggctcaaaa gaaaccattc gtgttcattt tagtccgaac
    23461 aaacgtcaat taaaattgtt tgatggagga aaataatgga aaaccaaaat gaaagtttaa
    23521 tcaagcagta tgttaagcgt cgtgcaaagc ggcgcttatt tttgtggttg tttggtactt
    23581 ctgcaggttt gattactatt ttgatcactg tgttcgtgac cctatttcta atacttgcgg
    23641 cgggctcgat tgataattcg gattcagatt caagcagtgg tggcgaagct tttacaggag
    23701 aatattcgga aggcttaccg atttataaag aaatcaaagg aagaggccct ttttctgatg
    23761 agattgcgca atatgccgta ggtgcagcag tgaaatataa attattacca agtgtgatcc
    23821 tttcgcaata cggttacgaa tcagcgtttg gtacatcggc gtccgcaaga aatgatctca
    23881 actattttgg gattacttgg tttgatggtt gtctgtttcc taaaggaact gcacgaggaa
    23941 ttggtggcat tgaaggtggc tggtacatga agtttcccaa ctcaaaagct gcattttcct
    24001 attacggctt tatggtagcc acacaatcca attttaatgc ttgtgtagga aataaaagcc
    24061 ctggcgcaag cttattgatc ttaggacgag gcggttatgc cgctgcagga attacggaag
    24121 atagtccgta ttatacaggt tgtatgtcaa ttatcacttc taataaatta accgaatatg
    24181 atgaattcgc catcaaacat tggggtgaag gtggcaataa caacggcacg attactggtg
    24241 aatggacgaa tccattccct ggcagcagtt tagataaaag ttccttcagc ggtggtcaat
    24301 tatttggaac gaatccaggc ggtgaattcc gtccgaacgg cttccatgat ggattagatt
    24361 ttggttcagt tgatcatcca ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt
    24421 acgttggcaa tcctggtatt tcaggtttgg gtgcgtgtgt gatcgtgatt aattacgatg
    24481 gcttgaatat ggtgtatcaa gaatttgcaa atagtacagg aaattctcga gtgaaagtag
    24541 gcgatcaagt gaaagttggt caagtaattg ggatacgaga tacagcacac ttgcatttag
    24601 gatttactcg aatggattgg cgtcaagcac aaggacatgc attcattgat gatggtacat
    24661 ggatcgatcc gttaccattt ttgaatagca gtaaaaaata gatcaggaga aaaaatatgg
    24721 aaaataaaac gttaaaaatc gtcttaacag ctgcaattag cgttagttta atcggcaata
    24781 ttgcgttgtt ttggcagctg aaagaggaac aagcagcaaa tacctataaa attgaagaag
    24841 tggctaaaaa cgcgtctgag aagctcaaaa aaaattcctc tgaggcacag gcgaagatcg
    24901 acaagttaaa tcaaaccatc aatgagaaaa accaagaaat cgatcagctg aagcaacaag
    24961 atggcacaaa agcaaaagag gaagtaactg aagcacaaag aaagacggca gaagagttcg
    25021 gtcaattggc cattgataaa tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg
    25081 tcgcaaccca agaagtgatc gacaaattaa gtcctacaga cgatgatcac caacatgatg
    25141 attacggaag ttactccttt acattaggtg acgtgcaaac ttacgctcaa acaagcagtg
    25201 taaaagagga acaaaatttt gtcgtatttg ttgattatac aatcggcaat ccgcaattca
    25261 aagatgtcaa accacaaaaa ataaaaggtg gattaactgt cactgaaaaa caagtagatg
    25321 gtcaatggaa agtcatagac tttagttatt tcactcgtta ggaggaagaa aatgagtaag
    25381 aaaaaaggag aaagacaacg tttcaaaaaa cgaaaaagtt atcttagatg gctgggaatc
    25441 tttatgattc tcggcttttt gattcttgct agtccgattt atacgaatca gaaggtagat
    25501 ataccagatg aggtaagcac gaacgccttt ttaaacacga aagagctgac aatggtttca
    25561 aaagaaaaag gagcgaacca gcaattagtt tttgaatttg cgatcgatga tgaagatcaa
    25621 agcttgttaa aagaactggc taatcttaat tatcgcgtag aagtaaagac agctaaaggg
    25681 gattatcaag caatacgagc caaagtgatt aaggtgtctg atgattattt tgcagtgaag
    25741 ctaacgcatg taccagaaga atatatcgcc atgcgtttaa ccattattcc tgaaaaaatt
    25801 gatccaaaag tggatatgca agaaccacaa gatctgattt tttatattca cgaagataag
    25861 gtcaaagatc acgtgaaaga cgaagattat gaacaacatg cgatcaatta caaagtgaaa
    25921 ggatacaaga aagaacaaaa agcagctcaa aaacaaatgg aatctttcca agcaaccatt
    25981 gatttgaatg aagaattagt gaagaagcta aaagatcaat tgccttatca agtcgcagat
    26041 gatcaggaaa atactgaaaa caagattaat agctaccagc aagaaattga aacatcgaaa
    26101 acgcaactga aagatcaaga agagatcatt caaaaacttc aagaaaaaat tgatcgtatg
    26161 acaaaagaaa agatttagaa ggacaggtgg agtgaaaaaa tgaagcgaac aaacttggct
    26221 gacaaagata ctttcgctga gcagaaagta ttgattaaag cctatctcaa agttgtaaaa
    26281 gctaaagaaa tatgtcgaga agtgaattta tctaacacaa caatatctga gttgaagaat
    26341 ggtcgaaaga atttagatac cttaaaattc agatcatttg aagctctata tgtagctgct
    26401 aaaaaatatt ttacagctga taacaaataa aaaacaggag tattttatgt gaaaaaagta
    26461 tataaaaaat gcttatgaat cagctatatc accttaacca tttgttaatg agatgggaga
    26521 aaacattaac cacgattcta acgcaaacat caagcacata aaacatttaa cactcctcct
    26581 ttctggctga agatttcaac caaacaggaa gacagcgtat caattatagc atacactttt
    26641 tcttaaattc atttttcata tgacaaaata tttccttcta atcgacaaag ttagaaggat
    26701 tttttattgt ctaaatacac aaataacctt aatttttaaa gagcataagg aaacgtgata
    26761 tcactttctc acgaaaggtg ttaggtaggc tgtgcctacc acgtttctaa gaaagataaa
    26821 tgatatcaaa attttggatt tgtgtgtaga tttgtgggta ggatgaaagc gaatgtgggc
    26881 agtaaaataa gcgtttgtgt gtagcaaaat caaaaaatgt gtagggatat gtgtagctac
    26941 acatatccct acacataata aatttggagg aaaatatgga cttattaatt agaaatattg
    27001 aagaaaagta tattaataaa attgacaaac gttgtgaaga acttacggtc aagactggca
    27061 aaaaatggag tcgaaatcaa tacctaaaag ttttgatcga aaatgatttt gatcacgctc
    27121 ttctgaatta taaaaaagac caatttgatc ggttgttaga aaaatttgtg gatattcaaa
    27181 cttataatac gaaaacttta gaagaatata ttgcaacaaa taatcaatta attggtttgt
    27241 taattgaata ataaaaatag tattgtgaat ttataaaagg aggaaatcaa ttttggcaaa
    27301 agcaacaaga aaacatgtat atctttacga tatgcatatc gaagtacttg atgaaataat
    27361 ttctactcat gaagatatat ctaattatag tgaagcagtc agacatttgt gtatgatgta
    27421 tgatccaaaa atcgaagaaa aaataaatcc aattcaaatt aaattgaatg caatgggaaa
    27481 agaaatttct attttaaccg aaatggtttc agagtttgcg gatcttcatt tgaaagatac
    27541 aggaatacta attggtacaa attcaactgt ttatcaagaa gcagaagaac gtgtggaagg
    27601 aaaaattaaa tccaatcaaa ccaaaaaata ttcttctaag ttaaagaaac cagtgaatga
    27661 atctgttcat accagagtag atccatttga tccaacgaag attctaaaaa gataaaagta
    27721 gaaggagaac gtgatgaata aatcgcctgc agttattctt acttgtcaat ttaccttgcc
    27781 caataaaaaa tctttttcaa catacattga ttatatgaca agagaaaaag cactggaaga
    27841 aatggaacga cgtacgcccg aagaagaaca ggaactgcaa cgaatcaaga atgctttaga
    27901 ggatttttat attcctgaag gagaaacttt ttcggaaagt aagacggata aagaattcag
    27961 tgaaacgtcg aatgaagctc aatcgttgat ggaacatgga atcaattttg acaagttaga
    28021 ggaacaagat tttaccaaat atctttctta tatgacccgt cactatgcgt tagaacaaaa
    28081 gaaagaactg acgcaaaggg aacaaaaaga ataccaacgg ttgaaccaag caatgaaaaa
    28141 atatcaaaca gagccacata aaaaagcttc aggaaatctt cctggagttt tttctatggc
    28201 ttcagacgaa gtgaaaggaa gtgatctgaa ggagatcaaa caaatcttcc aaagaggaca
    28261 acaaaaaggc tcaatcattt atcaagatgt tatctctcac gacaattcgt atcttgaaaa
    28321 aatgggcttg tacgatccca aaacagatac cttagatgaa gaaggtttga aagcagctgg
    28381 aagaaaaatg atggagacat tattcgagaa agaacaattg aatgaaacag gttactggat
    28441 ggcaacgatt caccgtaaca ctaagcatat ccatattcat tttgcagttg ttgaaaaaga
    28501 aaatacgcgt aaaccgtata ctgagattga aaatggtgtg agttatatcg taccgaaggg
    28561 gaagagaaga caagcaacat tagacgcaat gaaaaccaat tatgtacatg agttagaacg
    28621 tttctcttat gaaaaggatc gaatgatcgg tatggagaaa cgaaatttat tgacacgaaa
    28681 aagtgatttg cgtaacacct taacaaaagt agtaaaagaa ccagcacgtt acgatcgaca
    28741 cgccatgaac ttattaaatg aagtatataa aaccctacct gaaaaacgtt cggaatggaa
    28801 ttatggagat gaaaatcgaa ccaagctttc accatttaca aggggaaaac ttgatgagtt
    28861 aaccaaatat gttttaacaa aagaacctgg atatcaagaa tatatacaga tttcaaaagc
    28921 attgaaagaa gaagccaaaa gcctttatgg ggaaagcaaa agagaaacaa agaatgcaga
    28981 aaaaaacgcc ctctttgatt taaaaaagcg cacaggaaat gcgattttaa cagaactgaa
    29041 gagacaggat agtcaaataa aaccattaat agaaagtgtc aatcagatgc aatctattta
    29101 caatgaacga tcctttcgaa cactcaaaca gttgccaaca aatgaataca agtatagtta
    29161 tcaagaattc gtaaagcgta ggaaacaatt ccaacaaaga gttgtcacgc agcgaagctt
    29221 gcgtcaatta aatcgaacaa ttaatgcaca tgaagaaaaa taccacgcaa gtaaagcata
    29281 tgaagagcaa cagcgaagaa tccaaatgga acaagaaaga caataggagg aagagaaatg
    29341 aaacaatatt tagatcaatg gaaagtcata gaaggatcac taaaagaaga acgaatcgaa
    29401 caacttcctg attgtttaga aaaagagcat ttgtttcaga taagcgaaat gttgagaaac
    29461 gaacagtttg atccaaatca atttttagtt gttgagtacc cagctactgg tgtgtattgt
    29521 tgtaatcatg tgaagggtga aaaatatttt attattcaag aatacgaagg taagttagcg
    29581 ccttattata ctacttggga aataaatgaa gagggaatca ataatttccc atgtgaaagc
    29641 attgaagagt ctatttctct cacggaatgt taaataaata gagacgtaaa agagtcctga
    29701 acgtgattgt ccaggactct ttttcaatga attgttttcc gccaattcac tgagtggtgc
    29761 gattcaattt tattatatca aaagaaagtg agattccaaa gtgaagggag gaagaagaaa
    29821 tgactcagac aacgattggt gttgtggttg aaaaaagcag ggataatctc gtttttgtga
    29881 cagaaattca aacaggtaga gcttttgtag tcacagacaa agctgcaaaa gcgtatcaaa
    29941 atggcaatat tttaacgtta aatctgacta cgaagatgtt cgttgatgca gtagaaaatt
    30001 atccatttgt ttagacaaac gatattttac aaggaggaaa cggacatggc agaagaaaaa
    30061 gaaccactag ataatagtcg gttgggaaag tccaaaagaa aattagtacg gttgcaaaac
    30121 gaattaaata atcaatttca aaagctattt gatcaccaaa aattgacaaa tgggcaacca
    30181 atgaatgaca aacgaaatgg tcgttcatgg tttaaccagc aagaaaaaat agaaaataaa
    30241 atacaaagtt tgagagaaga gatcaagcaa caagaaaaac aggtagaaaa acttgaaaga
    30301 caagaagagc taaaggaaat gggctataac aagtacggcg gactggatat gacgatcgat
    30361 aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa aaggtgaatc gactttttca
    30421 gccgccacga tccgaaaata tcaacggaag ttggaaacat tagaacagct gaaagaaaaa
    30481 tcagaaaaag gaaaagaaaa tatcctccct gaagtacaag cgatcattga cagcggacga
    30541 gtgacgcaat ggaaaaaaaa tccaaccatt tattttttga aaggctatcg aaaagttgct
    30601 ttagaactga atgaaaacgg gggatttgag gaatcaaaaa aataccaagc cacgtcggaa
    30661 gaggaaaaag cagctgttcg tgaattactt gaagaggctg caccaactga ttctctgaag
    30721 gagaaagaaa aagatcattt ctatcgagta gaattcaacg aaaatgatcc agaaaaagga
    30781 ttgaaaagtt atggtgggga gatcgtaaca ttagaattga tcgatcaact taaaacgttg
    30841 gataaccaat acgctgcaga agcaggttat tataaattct attttgaaga agtgaagaac
    30901 ggagaagtag tagatcagat ccgaatggat ctaggggatg gcgtcaacag taatcacccc
    30961 atctatttag agttagcaaa attgtgcgaa caagtacctg agaaggaaca aaaacaatcg
    31021 gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga aagaaacaaa aacaaaacaa
    31081 aagggctatc aaaaacgaac ggcagaagag atcaaacaag aagttaaatc cctttcgcaa
    31141 caggcactgg atgcggtgaa aaagcatacc cattcttctg aagacgtcaa agaacttttg
    31201 aactttatgt ctcgttttcc agaacgatcc tttagaaatc aattattgat tgaggaacaa
    31261 tttccaggtg caacggcttg tttaggtcgt gccgcgttga aaaaagaagg aatctatatc
    31321 aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa cggtcaaagg attttatgag
    31381 aaaggacgtg gctttgttcg agaaacagaa gctacaccag aagataaaaa acgaatcgaa
    31441 agtggtcaaa tcgaagtgat caaaaaacct tactacacga ttgaaaaagt gtttgatatc
    31501 acacaaacac agttaaaacc tgaagactat ccgaaaatct ttccgaaccg tgtctttgat
    31561 tttcaattgg ataaaaaagg acagtcagaa cttcaagctg ggattcaagc ggtagccaac
    31621 aatattggaa tacaaattcg agatatgaca gaaagtgaag tctatcaaag agaattagga
    31681 caagcacgtg gggcgtatgt tcgtaacgaa tttactggcg aagaagaaat cgtcatgaat
    31741 actcgaaata ctcctacgca gcatttagct acaagtattc atgaattagc ccatgcaaga
    31801 atgcacaaat tttcagaatt agataccgca actaaagaat tgcaggcaga aatgacttct
    31861 tatattgtgt gcaaacattt tggaatggac acttcggaga aagctattcc ttatattgca
    31921 gcatggacga agaatggtca gacgttggat gataaggaag cggcggaacg tgggaaaatc
    31981 atgaatgatg tcagccgtgt cgcaaatgaa ttcattcaaa ccatttctaa tgaaatcaat
    32041 caatacagag aaagagaacc agagatccaa ccagcagaag tgaagcagga gcataaaaat
    32101 actgtagaaa cggcagagaa taagaatatt gtgcccgttg gttcactttg gatcgacaag
    32161 aaagacggga aagtaatgga agaagatacg ggtcgaacgt tacatttgaa ggatgcggcg
    32221 tttattgaag atccgcagca taatcaagtg attttaaaag gaaaaagtta tggaaaagag
    32281 attcaaacgg cgttaccttc agaagaattt gcgctaggaa atccattccg attttcactt
    32341 gaaacagtgt cagaagtggc caaacgaaaa aatgaaatct attatgaaca acaattgtca
    32401 caaaaagaac gcgtataaaa caagtaaaaa agactatctg aaaaacggat agtctttttt
    32461 cttttgggag gtttgaaaat gaccgaaaaa aaaatgagtc ttattgatcg atgtaaacag
    32521 atcgatatag tggattttgc tcgaaacaat ggaatggctg tagtcaataa aggacgagac
    32581 tatcgcttag aagaccatga ttcatttgtt tttgatcgaa gaaaacaacg tttttattgg
    32641 aacagtcaaa atattagtgg cgacattatt gaattagcta aacttttttt catagacaaa
    32701 gagatccaag actctaagca acaatttaaa gcagccactg attttattct aaagaatgaa
    32761 gacaaaacag aacgagtgga aaatcttcat tttgaaacag aaaagtacaa agatcacccc
    32821 gttgattatc aaccactcac agaaaaagga aggaattatc tgaaggaaga acgaaaacta
    32881 ccagagtggc tgattgatta tgcagaaaaa gaagggttga ttgccgaact aaagccaaaa
    32941 tatgaacggc aaaatttctt agttcgagac gatcgtttag atcatgcggt cgccttttta
    33001 tggaaagatc cacagacaaa agagacagta ggggcgagtt atcaaggaac gttcattgat
    33061 tatgaacgtt ttggtgagag aggcacatat aagcatatag ataaaaattc gacagcgaat
    33121 catggcttta acctaaagat cggtgaccct aagcagttaa aattttttga gagtagtatt
    33181 gatttactta gctatgctgc actgaatcgt gatcagttaa acgatacgtg gttagtctct
    33241 atggaagggt taaaacacca tgtgatcagt cattattttg gtgaggcagt atctgaattg
    33301 agaaagaaac aagcctttcc tcaatccatc gagatttgtg tggataatga ccgtgcagga
    33361 catattttct acgaaaaaga acaattaatg ggcgcagtcg atccatttac gaatcagaaa
    33421 gtacgttgtg aacgaggaat cgctaatgat tggcaagtgc caaaagaata caaaatcatt
    33481 tatgaagaag tcgcaaaaga agaaaaggtc acaccagaag ctattatggc catacacaag
    33541 acagaaaaca atctgcaatt aacgaaccaa ctggtttccg ctcacaaagt aaatgcgttt
    33601 tttggccagc agctttctgt taacgattca atagaagcca ttaatttgaa agatatttgt
    33661 agagaagtcg caaaagaatt aaaagtttgt gaacgcgtgg atggtacgta cgattttgat
    33721 cgtttttatc aagaaaaagg ggatattaat gcgcagattc ttttctctta taaagcagaa
    33781 caatactaca aaggctataa aaatcatgaa catgaattta tacctgaagt aaaaaaggac
    33841 tggaacgatc aattgaagca tgagatccag caacaagaaa tcagaaagca aaaacgtgcc
    33901 atgttgtttc aacaaggcag acaacaagaa agggagtgaa atcgtcatga tgaaaaccgt
    33961 tattttagca gaaaagccgt cacaagcgaa agcttatgcc gatagctttt ctaaagctac
    34021 ccgaaaagat gggtattttg agatccaaga tcgattgttt acaggagaaa ctgtgatcac
    34081 gtatggtttt ggtcatttag tcgagttaga ttcgcccgat atgtacgatg aaaaatggaa
    34141 acaatgggca ttagaacatt tgcctatttt ccccaatcaa taccactatc atgttcctaa
    34201 agataagaaa aagcagttta gtgtcgtcaa acgacagctg cagtcagctg atacgattgt
    34261 gatcgcgact gatagcgatc gtgaagggga gctgattgcc tggtcaatca ttcaacaagc
    34321 aggcgccaat caaggaaaaa cctttaaaag actttggatc aactccttag aaaaagaagc
    34381 catttatcag ggattccaac agttacgaga cgcagaagag acctatccaa aatttgaaga
    34441 agtacaagca cgtcagatcg ccgattggtt aattgggatg aacggtagcc cactgtatag
    34501 tttactcttg cagcaaaaag gcataccagg aagtttttct ttaggtagag tccaaacccc
    34561 aacactctat atgatttatc aattgcagga aaagattaga aacttccaaa aagaaccata
    34621 ttttgaagga aaagcccaag taatcgctca aaatggcgca tttgatgcga agcttgatcc
    34681 aaatgaaacc caagcgacac aagaagcatt tgaagcctat ttaaaagaaa aaggtgttca
    34741 attaggaaaa cagccaggta cgatccttca agtcgaaaca gagaaaaaaa gtgcggctag
    34801 cccacgtctt ttttctttat cgagccttca gtctaaaatg aatcaactca tgaaagctag
    34861 tgccaaagat actttggaag ccatgcaagg gttgtatgaa ggaaaatatc taagctatcc
    34921 gcgaacggat accccttata ttacggaagg ggaatatgcg tatctgttgg atcacttaga
    34981 cgaatacaag cattttttaa aagccgaagc gattccgact cctattcata caccgaatag
    35041 tcggtatgtg aataataaga aagtccagga acattacgcg atcattccta caaaaacggt
    35101 tatgacagca gcggcttttg aacaactaag tccactgcag caggctattt atgaacaagt
    35161 gttaaaaaca acggtggcca tgtttgcgga aaagtatacc tatgaagaaa caacgatctt
    35221 gacgcaagtt cagcagcttc agttaaaggc aatcgaaaag gtgccgctag atctaggttg
    35281 gaagaagtta tttggaaaag agagcgacgg aaaagagaag gaagaagagc cgctacttcc
    35341 taaagtgaca aaaggagaaa tggtgacggt ggatctacaa gttcttgaaa aagaaaccaa
    35401 gccaccacag ccttataccg aaggtacgtt gatcacggcg atgaaaacag ctgggaaaac
    35461 cgttgatagt gaagaagcgc aatccatatt gaaagaagtg gaaggaatcg gtacagaagc
    35521 gacacgggcg aatattattg aaaccttgaa acaaaaggaa tatatcaagg tagagaaaaa
    35581 taagctcgtg gtgacgaata aagggatttt gctttgccaa gccgtgggaa aagagccgct
    35641 gcttacgagt gccgaaatga ccgccaaatg ggaaagctac ttgttaaaga tcggtgaacg
    35701 aaaaggaaca caaaccactt ttctagccaa tatccaaaaa tttgtctctc acttattgga
    35761 agtcgtacca gggcaaatcc aatccactga ttttggatcg acgttacaag aagtgaaagc
    35821 agccagtgaa aagcaagaag ccgcgcgtca tttaggaatt tgtccaaaat gccaggaaca
    35881 agaagtctta ctctatcaaa aggtggcagc atgtacgtct gaagcctgtg atttcaaact
    35941 atggacaacg attgccaaaa agaaacttac agcgacacag ctgaaagaga tcattcaaaa
    36001 tagccgaaca agccaaccag tgaaagggtt aaaaggtcag aagggttcgt ttgaagcaac
    36061 gattgtttta aaagaagact tcactacagg ctttgaattt tctgaaaaga aaaaaacgaa
    36121 cttcaagaaa agaacacgac gcactacaaa ataatggagg gttaatctta tgcgtaatac
    36181 aacacacaaa caacaagaaa tcgaaaatca aaaagccaaa attcgttcag aacaggaaaa
    36241 aatgcgtgcc agcaaacgaa gactgcagca attgaagaac cagcagcgga ttcaaaaaga
    36301 aatcgaatta gatcgataaa ttcctttctt gttaatataa aactaacatg ttatagttgt
    36361 gttagtttta tattaacttt aggggaagag taatgaaaga caaaatccaa tgtatttttg
    36421 ataaacataa tggcaaccta tccttgaagg atgctagaag tgaaggatta tcgcctatga
    36481 cgttagatcg attagccgaa aaggataaaa tagaaaaggt agcaccaggt ttctatacct
    36541 taaaggatgc gttcgtggat gaattgtatc tcgctcagag tatctattcc agagggattt
    36601 tttctcatga gactgcgctc gatctttttc aagtaggaac atatatccct aaaaagatca
    36661 atatgatgtt tcctaaagga tacaatgtat caaaggagaa attagatcag tatgccattc
    36721 aaccgcatta cactaataaa gaaaattttg acctgggagt cacagaaaca gaaagctttt
    36781 acggcaataa aattttagtc tatgatttag agcgaacgtt atgtgacatg tggaatccaa
    36841 gatacaaagc aagcgtagag gtaaaacaag aagcattaaa agaatatatg acaagtacca
    36901 gtagaaatac aaataagcta agaagataca tggatatttt aaaatcacca aaagaaatga
    36961 ctttgtatat gttgccactt tattgagagg agtaaagatg aatgacaccg acacaattta
    37021 aagagaaaac aagaaatttc gcagcagaga aacatttaaa cccacaaatt gtccaaagat
    37081 attatatgat ggaaaaatta ttagaatgta ttgttgaatc gccttatcat gatgattttg
    37141 tattaaaagg gggatttctg attggatcaa aatacgggtt agaaaaccgt tcaaccgttg
    37201 atattgatac gacttatcga aattcgaaac taacaaaaga aaagttagaa actgtttttt
    37261 ctgaattaac agctaatcct acaaaagatg gaattcgttt tgaattaaag ggattaacag
    37321 agacgaggga agccgattat tatccaggtt ttcaagcaaa gtttattgct tatcttgaaa
    37381 atgcacgtgt accttttaaa ttagatatta cttcaggtga ttctattatt ccagcagcat
    37441 tgatgtatga acacaaatta atgtttgaag ataaaacgat tcatattcca gcttatccta
    37501 cggaacaaat tctagctgaa aaattacatg ccacgtttag ttttggaaaa gataattcgc
    37561 gcatgaaaga tttttatgac ctgtatatga ttccgaaatt agaacgtatc gatgagaaaa
    37621 atttgtataa gagtgttcaa ggaacattca aggaaagaaa taatcaatcg gtgtttaagc
    37681 atttgtatga tcaggaaatg cctcaattgg tgaaagatga gggcttgaga gaaaagtgga
    37741 aaagatacca acaagaaaac tattttgcca aagacatttc tttcgaatct acactaactt
    37801 ccgttgattc cctgatgcaa tcaattgtag agcaagagaa ggcagaacga aaacaaaatt
    37861 ttaagagatc acaacaaatg aatcaagaaa tggagagata aagatgatca ataatgtaac
    37921 tttagtaggg cgattaacta aagatccaga tttacgttat accgcaagtg gcacagcagt
    37981 ggcgactttt tccctagccg ttaatcgtaa tttcacgaat caaaacggca atcgagaagc
    38041 tgattttatc aattgcgtga tttggcgcaa accagcagaa acgatggcga cattagctaa
    38101 aaaaggcagc ttgatcggcg ttgtcggacg aattcaaaca cggacttatg acaaccagca
    38161 aggacaacgt gtgtatgtga cagaagttgt ggccgataac ttccaactat tagaaagtaa
    38221 agcagcgaca gaaagccgta tgcacgctga tcaatcgtcc agcagtccaa gccctaccac
    38281 ctttgaacaa cgggatacag cgacgcctaa caacaatgga ttgaacgctt cacagaatcc
    38341 atttggtgga caatcaatcg atattagcga tgatgatcta ccgttctaag tagcattttc
    38401 tattggttgc atttgctacg gtttatgtta taatttagac aacaaaaaga attttcaatc
    38461 tataaccaaa aaccactagc tggcgggcta gtggctttct tttacgtttc tattataaga
    38521 attaacgggt aggcttactg tctttcttgg caaatttaca atcaagccat ttgcagatat
    38581 agtgcgcggc tacttctgcc atgattgcaa aaacaaaatc aaaaagaatt ttcaaaccta
    38641 taacctcctc cccttgccag attaggccgg ggtagcagac agcaagacta ttatagcata
    38701 attgaaatat ccaaatgatt ggatttttgc tataatttta gcgaggtgat tgtgatgaac
    38761 aaatatgttg gtttgcttga taaaatccga gtaataaaaa cgcaaccgtt attggttcgc
    38821 tttactttac aaacgattca tgaatcgatt aattgtgtag ttgcagatat tgaaattatc
    38881 gataaattgc taattatgga tgatggaaaa tacaatattg ccgtcacagg tcacttcaat
    38941 aaacgaaatc agctagttat tgctagcatg tatgttagaa atcccgatca ttttacgcga
    39001 tcgatgggca tataaataaa agaagagttg tagtcctaaa tgaggattgc agctcttttt
    39061 gttttacata gaaacaggaa aagacctttt taggaagaca caagtgcctt ctcaattgaa
    39121 aaacctttct ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt gaacgctgca
    39181 cgttcactaa gatatggtgt cttgcaaagc aagacaaaat agacaaaact tcctctcacc
    39241 aaaaggttcg ttcctgttcg ttatctcttt attgatttgc cagaccggca acaagttgcc
    39301 ttttatcacc aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc ccttgtcctt
    39361 gtcgcttttc gttgatttat tctggccaac aaatcaataa agaaagaact aacgaaagga
    39421 agtaaaacct ttcctaagag gaggcagaat caaacatgcg aaagaaattt gattattggg
    39481 gtgttccctt cagcgaatta ttcccaaact atcatgcacc acatactgtt gaatgcgatt
    39541 gtggagaaag agcgaaatgt atcaaaagct atcgtttgta tcaatgtcca acatgcggga
    39601 aaaaatatac cttgtcttat ggcgattaca ttttgattga tgagaaagga aagaaacgct
    39661 gatgaaaaca caacacaaat tattgactgt aaaagaagtc gttcaaattt tggtggacaa
    39721 agaagtcatt tctgaaaaag tagcagaagc tttacttatt ccgcggtact ggacgataga
    39781 aaccccttta gtagaggatg caccgactat gctatttgaa gacctagtga agattgcagg
    39841 atatgaactg cattatcaag aaaacatgga aacttatgaa atcgaatatt ggttaaccaa
    39901 attagagaac taaaaaggag aaaaaatatg gctcaaaatt actatgacga atttgtcaaa
    39961 ttacctttgg ataaaatggc acaaaaaatg gaggatatga cgttcctata ccatgaaaca
    40021 cgtgtaccca aaaaacatta caaggaaaaa ttgtcagtcg cagtggaaga aatgattgaa
    40081 tcaggtgtcg aaatgaatct cattgcgacg tactatcgaa cactggaaga actgaaaaaa
    40141 caaaatggaa aatggttttt ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc
    40201 actatcaaac cttccgaata tcaagcctta gaactgactt atgcaaaatt cgcacagatt
    40261 aagaaagcta aagcaatggc tagcgactgg ctagatactt ttgaaagtat cgaaaaaaat
    40321 ggtgcatttt atacgattga atcggaggag aaagataatg aataaggtgg tttatcagct
    40381 aacaacagaa gatgtttcgc aatttttaac aagcaaagaa cacttgaatg aagtaatcag
    40441 acggattcaa aaagacttta gttgtatgga agaaatgaag tactttattc aaagagtgga
    40501 agaacagttg tatatggaaa gcgaaacaag tcaaacgaag gtcacagtca gccataatca
    40561 tttagacgtg tataaacgtc gtcctgaaac ggtaaaaaaa ggaacagagc ttctacgact
    40621 agaaacagaa aaatacatca tgaatgtgta cctagaaagg agtgagctgg tcgaaaactt
    40681 tgtcagctac acgatttgta ggaaacacta ttactcagaa tcagaatatt attcaatttt
    40741 cgagagcatg gaattaatgg aagaagaaat ggaagagcta aacagcaaaa atgcgacggt
    40801 agacgattat taccttgtat ttactaggtt tttgacgtgt gggacggaat ctatcgtgtt
    40861 aatgggaatg gattatgaac tcgctgtacc cgaagcattt ttgagggaat atgtagaaga
    40921 agtagataac ccaggcgacg actggtacac gactttaact gattttttag acacctacac
    40981 ttacgacgat acagaagaaa tccaaaatga acttgaccgt aaacgaatgc catatcaacg
    41041 atttgaaagg aaaaaaggta aataaccatt tataccgaaa agtgaggatg tgtgaaataa
    41101 gaacaaaaaa ccttgaaatt aagggaatgg ttcttatttc gcaccaatcg ctccaaccgc
    41161 ccccgccgct taggtggtgc gaaataagcg ggcaaagccc gataacctaa gagaaagaag
    41221 gagatccata tgaataaaat cattcctcta ttgttcgtac tgtttgttgg cgagatactt
    41281 atgcgtcatg ctgcagctgt acatttaaac ttcgtagtat tcttaggaat actggtattt
    41341 tgtttgatct tttggcgatt tgtattagcc cttgtgagtc tactattcaa gatgattgca
    41401 gccatatttt tcatagggtt atttcgatga aaggagagag tactaatgcc aattgcaata
    41461 ggaaataaac ggttacccgt gacattagat gaaaagagac aaaaagaatt gcagcaacta
    41521 aagcagaagt acggaaaaag tgaatctaag attatgtgta ttgcgttaga tctattgatc
    41581 gcgcaagaaa aagcaggatt tgatatacca gcactaaaaa agtgacgtca cttttcattt
    41641 gaagaaacaa aagtgatagc acttaattgt tcctactatt tagaatattt tctatgtcct
    41701 ttttattcca ttttgtatca ataactatga aatctttaaa gtatccagaa gagattattt
    41761 ttagatcaat ttttttctta cctgattcta aagattttgt tttcttaaac atattatgat
    41821 ttcctttcga ctattttcat ataaatgtta acatagttta tggattcatg gaatgttaat
    41881 ctaatttaat agaaaaggat aattaagtat atacgttttt tcttatgtat gttatactat
    41941 gtatatacag aaagaggtgt ctactatgga aatgttacct ataaaaaaat ggggaaatag
    42001 taatggcctt agattgccaa agtatattat ggaatatctt ggaattcata cagaagacaa
    42061 agtgaagata atacaagaag aagtaaacgg tcagaagcgg ctaattatcg aagcggctaa
    42121 tatagaaaat gaattaacaa ttgaacagtt atttgaaaat tacactgatg aaagaaatca
    42181 tgtgacgatt caaaatttag gtgaagcagt aggtaatgaa aaatggtgag aaaacctaga
    42241 caaggcgata ttctattatt gaatacagca cctcgatctg gacacgaaca gacaggtaaa
    42301 agaccttata ttgttttaag tcatgatatt attgcggatt atagtaacgt tgttacagtt
    42361 gctccaattt ctagtacaac tagaaactat ccgctatatg taaatataaa ctcagaatat
    42421 gaaatgaaga caacaggaaa agtattatta gatcaactaa cgacaattga ttatgaagcc
    42481 agagagtgta tttttttgga aaaagcgcat gatagtttag ttgaggagtt attgatgaaa
    42541 gttagaacag tttttcaaaa agtaagcaaa taaaatagaa acagcagcat gatcaactat
    42601 tcgtgattga ccatgctgtc tttttttatt ctatatacgg tttgtctcgt aacattgtag
    42661 tcttttgcaa tcttagcgat cgcaacacct ttttttagat cctcaacgat gtttttataa
    42721 acaagacgac gttggggatc ttttgcattg gcactgtata gcttaggacg tcctttataa
    42781 acaccgttcg cttttgcaat cttaatgcct tgtgcctgac gtcgtttaga ttcggtacgt
    42841 tcttgttcag cgatcatggc caaaatttga atgatcaaat ctttaatgaa tttatccagg
    42901 agaggattgc caattgcttc ggccatgatc ggtagacttg tgatgatcag ctgcacattt
    42961 ttcttcttca agtaattgac tgaagcgatg atctcgtcgt aatttcttcc aagtcgatca
    43021 atagcttcca caataaaaat atcttgatct cggacgaaat ccagtgcttc ttgaaaaaca
    43081 agtcgatgag taatcgttgc gccagattgt ttctcaatga aaattctctc tgcaccgaat
    43141 tttttcattt cttcaatctg ccgttgttca ttttgatcga tggatgagac acgtatgtat
    43201 gcaactctca aaatttttac ctactttctg caactattta aaaaacaccc taatcataca
    43261 cgagaaaacc agatttttca atgtcgcggt cgtgtctagt tagggtatac cttatataga
    43321 caaaaactaa tgatggagga aatagtatgt gtcacttttc tttttaaatg gcaatatttt
    43381 ttgtaaataa atgtcaatca agaaaattta aacgattatg acattaaaaa tatattttat
    43441 gaattttcta gtatataact tgatttatct gttaagattt attaagaaaa cgttttctta
    43501 tttttgattt cgaggtgata gacaatgaaa aataagttta ttttatcttt aggtatttta
    43561 actattggaa ttagtgtaac tggaactaca attcctacat ttgctgatag tacaaatgaa
    43621 ccaactactg tagtagataa taaccaagaa ttaactcctt tagataggaa cggaatagcc
    43681 attggtaacc caactaacga cttaactgtt actgaaaatt atttaatagt ttctccatta
    43741 cttgctcgag cagcttctaa aacgatacga gtgacaggaa cgatgatttt tgataaagtt
    43801 ggttggcctt ctagacctcc tcaatcacgg aattggaaag aaaaaagaaa tggttattgg
    43861 tactatggaa aattgtattt acaaagttat aaatcagctg gaaacaattg gattgcgact
    43921 tattcaggaa ccttaactag aaagtaggga ttttttatgc cagaatttgt gaacacacca
    43981 aatttttatc aagatagcga tattctaagt aaaattaatt taccgaaaga agaatcagct
    44041 ttacaagttc agcgaacttt acaggagaca caaaaaattc aaaaatcaat ttctaatcta
    44101 gaagattctg aagaaaaaag aaaaaaaaga gaccaaaaaa catatcaaaa agaaaaataa
    44161 tgattttaaa aaaagagtgt ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa
    44221 aatcacccat ttctggacac tttctttttt tgctttattt ttcaaaaaaa caattcattt
    44281 catgttcata aacccataac aatatctatg ttcagttaaa ctatatggat aaagttatgt
    44341 tacaataaaa ttaatgaagg agtgattttt ttgaaaatag gatacgcccg ggtatctact
    44401 ggtttacaaa atttggattt acaagaagac agcctaaata aatttggttg tgaaaaaatt
    44461 tttactgatc atatgagtgg ggcaaaaagt aatcgaccag gattagaaat ggccattgat
    44521 tttgttcgct ctggcgatac acttgtcgtt tggcgcttag atcgtttagg tagaaatatg
    44581 gaagatttga tttcaatcgt caatcgactg aatgagcgtg gtgtgagttt tcatagtatt
    44641 caagaaaata ttacgatgga caaatcaagt tctacaggac agctcatgtt tcatttgttt
    44701 gcggcatttg cagaatttga gcgtaatttg atcctggaac gatccgcagc aggacgagca
    44761 gctgctcgtg caagaggacg gtttggtggt cgtcctgaaa aattgtctga acaagatttg
    44821 gaattactga agactttagt ggatagcggt acacctatta agactattgc ggaacggtgg
    44881 aacgtctcaa gaaccaccat ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag
    44941 acaaattctc attagttttt gtcatttaat gctgaaaatt tttgccattt atctagaaaa
    45001 gtgacaatag tatgagaatt ttttgtaagt gtctactgat aacttattct caaatgttgt
    45061 aaggatttta ttcatttgtt aatattaa
//
LOCUS       DAZKOB010000012        44886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000012 DAZKOB010000000
VERSION     DAZKOB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.012"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8M6_000690"
     CDS             212..604
                     /locus_tag="ACG8M6_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0456380.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8M6_000691"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8M6_000691"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0456381.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8M6_000692"
     CDS             2698..2943
                     /locus_tag="ACG8M6_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456382.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8M6_000693"
     CDS             3165..4775
                     /locus_tag="ACG8M6_000693"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0456383.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8M6_000694"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8M6_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456384.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8M6_000695"
     CDS             5067..5936
                     /locus_tag="ACG8M6_000695"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0456385.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8M6_000696"
     CDS             6101..7372
                     /locus_tag="ACG8M6_000696"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0456386.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8M6_000697"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8M6_000697"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0456387.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8M6_000698"
     CDS             8763..9026
                     /locus_tag="ACG8M6_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0456388.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8M6_000699"
     CDS             9471..9920
                     /locus_tag="ACG8M6_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0456389.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8M6_000700"
     CDS             9977..11395
                     /locus_tag="ACG8M6_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0456390.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8M6_000701"
     CDS             11395..12255
                     /locus_tag="ACG8M6_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0456391.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8M6_000702"
     CDS             12429..13202
                     /locus_tag="ACG8M6_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0456392.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8M6_000703"
     CDS             13349..13714
                     /locus_tag="ACG8M6_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0456393.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8M6_000704"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8M6_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0456394.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8M6_000705"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8M6_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456395.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8M6_000706"
     CDS             15560..16216
                     /locus_tag="ACG8M6_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0456396.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8M6_000707"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8M6_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0456397.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8M6_000708"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8M6_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0456398.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8M6_000709"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8M6_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0456399.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8M6_000710"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8M6_000710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0456400.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8M6_000711"
     CDS             21888..22202
                     /locus_tag="ACG8M6_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0456401.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8M6_000712"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8M6_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0456402.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8M6_000713"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8M6_000713"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0456403.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8M6_000714"
     CDS             23546..23875
                     /locus_tag="ACG8M6_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0456404.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8M6_000715"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8M6_000715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0456405.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8M6_000716"
     CDS             24863..25687
                     /locus_tag="ACG8M6_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0456406.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8M6_000717"
     CDS             25680..26027
                     /locus_tag="ACG8M6_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0456407.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8M6_000718"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8M6_000718"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0456408.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8M6_000719"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8M6_000719"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0456409.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8M6_000720"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8M6_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0456410.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8M6_000721"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8M6_000721"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0456411.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8M6_000722"
     CDS             31684..32451
                     /locus_tag="ACG8M6_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456412.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8M6_000723"
     CDS             32441..34483
                     /locus_tag="ACG8M6_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456413.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG8M6_000724"
     CDS             34764..34913
                     /locus_tag="ACG8M6_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0456414.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8M6_000725"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8M6_000725"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0456415.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8M6_000726"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8M6_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0456416.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8M6_000727"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8M6_000727"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0456417.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8M6_000728"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8M6_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0456418.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG8M6_000729"
     CDS             39330..40412
                     /locus_tag="ACG8M6_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0456419.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG8M6_000730"
     CDS             40412..41215
                     /locus_tag="ACG8M6_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0456420.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG8M6_000731"
     CDS             41285..42025
                     /locus_tag="ACG8M6_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0456421.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG8M6_000732"
     CDS             42036..43040
                     /locus_tag="ACG8M6_000732"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0456422.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..>44886)
                     /locus_tag="ACG8M6_000733"
     CDS             complement(43085..>44886)
                     /locus_tag="ACG8M6_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456423.1"
                     /translation="LRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVF
                     YTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEK
                     ILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPL
                     ISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAV
                     SEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIG
                     VLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAII
                     HCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKND
                     LLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEML
                     CSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRE
                     THAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMK
                     DVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14429 a         7832 c         9344 g        13281 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaat
//
LOCUS       DAZKOB010000013        40969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000013 DAZKOB010000000
VERSION     DAZKOB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.013"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1384)
                     /locus_tag="ACG8M6_000734"
     CDS             complement(<1..1384)
                     /locus_tag="ACG8M6_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456424.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTL"
     gene            complement(1395..2063)
                     /locus_tag="ACG8M6_000735"
     CDS             complement(1395..2063)
                     /locus_tag="ACG8M6_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0456425.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2193..2777)
                     /locus_tag="ACG8M6_000736"
     CDS             complement(2193..2777)
                     /locus_tag="ACG8M6_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0456426.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3108..3341)
                     /locus_tag="ACG8M6_000737"
     CDS             complement(3108..3341)
                     /locus_tag="ACG8M6_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0456427.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3356..4306)
                     /locus_tag="ACG8M6_000738"
     CDS             complement(3356..4306)
                     /locus_tag="ACG8M6_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0456428.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4303..5145)
                     /locus_tag="ACG8M6_000739"
     CDS             complement(4303..5145)
                     /locus_tag="ACG8M6_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0456429.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5147..5866)
                     /locus_tag="ACG8M6_000740"
     CDS             complement(5147..5866)
                     /locus_tag="ACG8M6_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456430.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6414..6704
                     /locus_tag="ACG8M6_000741"
     CDS             6414..6704
                     /locus_tag="ACG8M6_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0456431.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
     gene            6776..8002
                     /locus_tag="ACG8M6_000742"
     CDS             6776..8002
                     /locus_tag="ACG8M6_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0456432.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8090..8482)
                     /gene="rpsI"
                     /locus_tag="ACG8M6_000743"
     CDS             complement(8090..8482)
                     /gene="rpsI"
                     /locus_tag="ACG8M6_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0456433.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8498..8926)
                     /gene="rplM"
                     /locus_tag="ACG8M6_000744"
     CDS             complement(8498..8926)
                     /gene="rplM"
                     /locus_tag="ACG8M6_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0456434.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9460..9927)
                     /locus_tag="ACG8M6_000745"
     CDS             complement(9460..9927)
                     /locus_tag="ACG8M6_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0456435.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10047..11585)
                     /locus_tag="ACG8M6_000746"
     CDS             complement(10047..11585)
                     /locus_tag="ACG8M6_000746"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0456436.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11665..12351
                     /locus_tag="ACG8M6_000747"
     CDS             11665..12351
                     /locus_tag="ACG8M6_000747"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0456437.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12552..13079)
                     /gene="msrA"
                     /locus_tag="ACG8M6_000748"
     CDS             complement(12552..13079)
                     /gene="msrA"
                     /locus_tag="ACG8M6_000748"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0456438.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13163..13840)
                     /gene="pgmB"
                     /locus_tag="ACG8M6_000749"
     CDS             complement(13163..13840)
                     /gene="pgmB"
                     /locus_tag="ACG8M6_000749"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0456439.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13837..14967)
                     /locus_tag="ACG8M6_000750"
     CDS             complement(13837..14967)
                     /locus_tag="ACG8M6_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0456440.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14967..17150)
                     /locus_tag="ACG8M6_000751"
     CDS             complement(14967..17150)
                     /locus_tag="ACG8M6_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0456441.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17147..18172)
                     /locus_tag="ACG8M6_000752"
     CDS             complement(17147..18172)
                     /locus_tag="ACG8M6_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0456442.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18217..19044)
                     /locus_tag="ACG8M6_000753"
     CDS             complement(18217..19044)
                     /locus_tag="ACG8M6_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0456443.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19041..20108)
                     /locus_tag="ACG8M6_000754"
     CDS             complement(19041..20108)
                     /locus_tag="ACG8M6_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0456444.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20134..20970)
                     /locus_tag="ACG8M6_000755"
     CDS             complement(20134..20970)
                     /locus_tag="ACG8M6_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456445.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20967..21836)
                     /locus_tag="ACG8M6_000756"
     CDS             complement(20967..21836)
                     /locus_tag="ACG8M6_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456446.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21906..23189)
                     /locus_tag="ACG8M6_000757"
     CDS             complement(21906..23189)
                     /locus_tag="ACG8M6_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0456447.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23203..24888)
                     /locus_tag="ACG8M6_000758"
     CDS             complement(23203..24888)
                     /locus_tag="ACG8M6_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0456448.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25163..26188
                     /locus_tag="ACG8M6_000759"
     CDS             25163..26188
                     /locus_tag="ACG8M6_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0456449.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(26783..30436)
                     /gene="rpoC"
                     /locus_tag="ACG8M6_000760"
     CDS             complement(26783..30436)
                     /gene="rpoC"
                     /locus_tag="ACG8M6_000760"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0456450.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(30515..34132)
                     /gene="rpoB"
                     /locus_tag="ACG8M6_000761"
     CDS             complement(30515..34132)
                     /gene="rpoB"
                     /locus_tag="ACG8M6_000761"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0456451.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            34860..35828
                     /locus_tag="ACG8M6_000762"
     CDS             34860..35828
                     /locus_tag="ACG8M6_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0456452.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            35846..36751
                     /locus_tag="ACG8M6_000763"
     CDS             35846..36751
                     /locus_tag="ACG8M6_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456453.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            36748..37554
                     /locus_tag="ACG8M6_000764"
     CDS             36748..37554
                     /locus_tag="ACG8M6_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456454.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            37711..38679
                     /locus_tag="ACG8M6_000765"
     CDS             37711..38679
                     /locus_tag="ACG8M6_000765"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0456455.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(38996..39520)
                     /locus_tag="ACG8M6_000766"
     CDS             complement(38996..39520)
                     /locus_tag="ACG8M6_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0456456.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            39807..>40969
                     /gene="scm"
                     /locus_tag="ACG8M6_000767"
     CDS             39807..>40969
                     /gene="scm"
                     /locus_tag="ACG8M6_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0456457.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTS"
BASE COUNT        12055 a         8468 c         7267 g        13179 t
ORIGIN      
        1 tcaaagttga atcttctact ctaagtttta tatctttttt tattccacca ttttttaaat
       61 aaataattct attgcatgtt ttataaagaa attccggatc atgagtagct ataactactg
      121 cacccttttc ttttaagtct ttaataaggt cagcaatcct ttccatatta atataatcca
      181 acccacttgt aggttcatca aatagaaata aattagtttt tgacaatgaa gctatagaaa
      241 ccaataatct ttgtttttct ccaccggata aatcaaacgg taattgatct ttcattttat
      301 aaagttccat tttttttaaa attttttctt gctgatcttt actaactatt tcattcccca
      361 gggctaactc gctcctcact gattctgtga aaaattggta atccatttct tgcattacta
      421 aaaacggcga tgacaaattt tttttatttt cgattatctt tcctttggta gggcgaataa
      481 tcttcatgag tattcttagt aaggttgttt tccccacacc gtttttccct accaaaccaa
      541 taacatctcc aactttgaca tcaaaattta tgtcttttag aatattttca taagtaattt
      601 cttcaacttt taatacatct gcacttacct tttcgatata gggaacttca atcgaactat
      661 taaaaatgtc aaatttcctt aatgcgttat tgttttcagt ttttaacaac tgaatagttg
      721 tagtttttac agttctattt ttaacaaata gaacttgatc aattaattct ttcaaatagt
      781 aaaaacgatg ttcagctata acaatagtaa atcctttatt ttttaaatca caaagtaagt
      841 cagcaagttt cttagtcgct ttgaagtcca aatttgaaga cggttcatcc aatataatta
      901 atttagagtt tagcgataaa ctacatccaa tagaaatttt ctgccgttca ccacttgata
      961 gcgaaaaaat gtttttatct aatatatttg aaagatctaa taattcagct aattcattga
     1021 ttcttttatc catatgctct tttgtaaatc caaagttttc catcgaaaat actaactcac
     1081 tcgttgtatt tgttgtaaag aattgtcctc taggatcttg acttacgtag ccaatatttt
     1141 ttgatatact ttcttgtgtt aaggattgta gcgagttatt ttcaaataaa atttccccaa
     1201 ccaattttcc gtcataaagt cttggaataa gtccattcat taactttaat agtgtggatt
     1261 tcccactccc agaagaacca gtaatcaatg taagactgcc tttttttatt gaaaaattaa
     1321 ggtcaacaag cgcttgttcc ttaacatcct tatatgaaaa agaaacattg ttaaattcaa
     1381 acaattcttt cacctcaaat caaaatactt aagaaaaaaa tagtaacgga aaacaggatg
     1441 atatagtcta ataagctcat gtttgtttta tagtaagaac ttcttttcac aggagattct
     1501 atcccctttg ttattgcatt agcggtcatt tcttcagcaa tcatagttgc acgtataaga
     1561 acgggaacta acagaaattc caaatattgc agaggctgaa aaaaagacaa gcgaatgttt
     1621 ctcattttta aagattcttt aatcattctt atttcagaac gataagtcgg aaagaatctg
     1681 atcactactg taatacttaa cattattttt ttcggtaaat gtattttttc taaagaggat
     1741 aataattcat tgctagtata gacactaacc aacaaccatg agatcattac gaccggcaac
     1801 attcgaaata caatatataa ggatgtcgca aaaaatatag taaaaaactc tatcttgata
     1861 gcatcaataa ttttcattac taaaaacaga actgaataaa tcaagatgaa ataaatacat
     1921 gtctttattt tacgaaacat caatactaaa actgtagcaa taatgaaaca ccccacttga
     1981 aatatatttc cctttcctaa aaaaataatg accggaaaaa aaatatttaa agtaatcaag
     2041 gttaacggat tgatttttga cactctttca cctccaaaaa ataagatcgc actgttatct
     2101 taagctgata aaaatgcacc gttattcaga taacagtgcg atcttatcaa aaagcataac
     2161 tgagaacaat tctcaatgca agcttttttt aattaactca gtaattcaat ccacccagga
     2221 acagaaaatt gatacaatct tctggttaat agcatagtct tttcttttga atagctatgc
     2281 ttcaatatat catagaatgt agcgtagaat gagtaaatgt agaagtggat ttcttcttca
     2341 ttaaagacta aattttgatt gatcattccg atagtcttta acttatctag acaacagact
     2401 gtctttttcg tcacaaatga tgtaattttt tcaacaatat cttgatattt ggttccagct
     2461 gattggaata aaatcaaatc gaatatacgt ttattctcaa aaatataatt aataaatgga
     2521 tctaattcat tgaagttctc tctccaactt ttctctatat ctatattttc taagtttaag
     2581 aggtatttgg taaactcttc attatacata ttaataagcg cattaatatg cggctgaata
     2641 atttcttcaa ataacgcttc cttagaagta aaatgtttat aaaaagcacc cgtagttacg
     2701 cctgcggtca ggcacagttg ccttatctta gtttgctgaa atccttcttt ttcaaaatgg
     2761 cttagtccac tttctattaa cttttggtga gttataatat aatctctggt ttctatgggt
     2821 acttcctcct aataagatga attaacttta ttatcttaca caggacttga agtttcaatc
     2881 gttaacgttt attactgaac tcattcctta gagctaaact aatgagaaag agcagaataa
     2941 ctgatacaaa aagcaatcct acaagaatta atagactcat ctaaatcatt caccttttct
     3001 aaaataatcc gagaaagatt taaaaaaatt atatccagct attaggataa gtcctatcat
     3061 aatcactcca atagccattt tcttatcctc cctaaaaata aagtctttta cttaaaacct
     3121 tttttgtaat cgtcaataaa ttttttccct ttatcagtta attgaaatcc tatatctaaa
     3181 tagtcctcat atctaagttt agccagcatc tcacttacag atgctggact aacacttaga
     3241 acttttgcta aatcattatt ttttaatttc ttttttcttt ttgataattc ctgaattgct
     3301 agtaaataaa ttagtatagt agtcgtttta atttgtacca tacctaccct ccaaattatt
     3361 tcgaaaggcc ttcagcaatt ttatccaggt tccatttcat catcgcatag taactatcac
     3421 catcttgtcc tttttctgca attgaatcag taaaaatcgt tgaatagata ggaatattgg
     3481 tatcttttga tactgttttc attggtctat catccacact gctttctacg aataaggagg
     3541 gaacttttgt tgtgcgtaat ttttcaacta agtgttttat ttgatctggt gttccttctt
     3601 cttcagtgtt gatttcccaa atgtaagcag aaggcacatt atacgctttc gagaaatatt
     3661 taaagcatcc ttcacttgta acaatcattt ttttatcatt cggaattgaa gcaaatttag
     3721 atttagcttc tttgtctaga gaatccaatt tttcaatata cttatctaga ttttctttat
     3781 agaatttttt attatcagga tctttttccg ctaactgttt ttcaatattt ttagcgtaaa
     3841 taataccgtt ttctaaattt aaccaagcat gaggatcttc cttccctttc tcactctgac
     3901 cctctaagta aataacatct atgccatcac ttgctgcaaa atagtctttg ttttcctctt
     3961 tgttcgcatt tttaactaat tttgtaaacc aagcatttcc tccagtttcc aagttaacac
     4021 cgttataaaa aatcaaatct gcttttgaag ttttttgaac atcttcaggc aaaggttcat
     4081 attcgtgggg atctttccca ataggtacga tactgtgtaa atcaatttta tcttttgcta
     4141 tattttcggt aatatctgct aaaatcgaat tagtaactac tacttttaat ttttcgttcg
     4201 aagccacttt atctttgtct ttactattta ttgaacatgc cccgaatata aaaagagcaa
     4261 tcaaaatccc cagacttccc agtactgctt tccattttct catcataacg atctctcctt
     4321 ctttcttaaa aatttttgtt ttggtgagat tagaaaacta attacaaata agaatgccgc
     4381 tgttaaaaca atacttgatc ctgctgctat gttaaaacta taaccaataa acaatccaag
     4441 caatgatgaa cttgctccga ctgttgcaga aataaatatc atagttttca agctatttgc
     4501 atataaatat gctgtagctg ctggtgtaat taacattgca acaattaata tagttcccac
     4561 actttgcata gctgttactg ctactaaagt taatagaacc atcaatagat aatgataaaa
     4621 actgacattc atccctattg cttttgccat taatggatca aaagaagtga ttaataattc
     4681 tttaaaaaat agaactacta taaccaacac taaaaccgaa acgccaattg taatccactt
     4741 atcaatatct tgtacagcta aaatatttcc aaataaaata tgaaataaat ccgttgagct
     4801 atttgcaact cctataagga taactcctag tgctaaaaaa gaactgaaag taataccaat
     4861 tgctgtatct ccttttatta cactattgct ttttataaaa gtaataatga tagaagctaa
     4921 tagaccaaat acaatcgcac caataaaaaa attaatacct aaaatataag aaattgctac
     4981 tcctggcaaa acagcatgag aaatagcatc acccataaga gacattcctc ttaaaataat
     5041 aaaacaacct actgttccag aaacaatccc tataactaca gatgtaatta gagcattctg
     5101 taaaaagtga aagtcataca gaccattaat aaaattttga agcatattat tccccctctc
     5161 caataaatat agtatctcca taggcttttt ttaaattttc ttcattaaat acagattcta
     5221 cacttccgtg agcaattaga tttcgattca aaagaataat attatcaaaa tacttcttta
     5281 ccttgcttaa atcatgatgc acaattagca ctgtttttcc ctgatgtttc aattttttta
     5341 acgtatccat aattattcgt tcactaacta agtctatccc tacaaatggt tcatctaaaa
     5401 atatgaaatc cgcattttga gcaagacatc ttgctaaaag tacacgttga aattgtcctc
     5461 cagataattc tcctatttga tgattagaat attttcccat atcgaccttt tccaaagcct
     5521 ttgaaactct ttgccattct gcatttttaa ttctttgaaa aactttcatt ccggcatatg
     5581 ttcccataga tacacattct tttactttta ttggaaaagt aaaatcaata tcagcttttt
     5641 gctctacata tgcaatcttc accagttttt tattgatatc ttcattatta atggagacct
     5701 ctccctgatg agggataata tttaacattg ctttaagcaa tgtggatttt ccagcaccat
     5761 ttggaccaat aatacctgtt aaagcacctt cttgtatttt taaagaaatc tcgttcaatg
     5821 ctaaaaaatc gttataacaa acattaagct ttttcacttc taacataact ttctccttct
     5881 aaatgataat gattatcacg atcatatggt atacaaaaat tatattttag tcaaccctaa
     5941 attttatagt ttagattttt ttctgttgct gaagattaga atgacaaaac tatttgaaac
     6001 tacgcgtata gtaagtagct ttcaaaatat ataaaaaagg agcgttctca aatgagaaca
     6061 tgctcctaaa actactttat tggcgtatgg aaaaattata cttgaagtgg atagcgggtt
     6121 gaataagttg ataagcatca aaacatttat aaaaattatt caagactatg tctgttccat
     6181 atacaaatat gaaaatcagt aaactccatt taactttttt cgtagtgtgt ctttttatac
     6241 atgtttatcg ttattaatag tgaataggaa aaaaactatt tttgatcatt gacaattaaa
     6301 tacaattttt gaataacgat ccctgctatt cgagctagaa actcaagtac gccatgacct
     6361 gattagcgac aaatacttag agatttcttt ttgttctggg aaaacaaaat gccatgacaa
     6421 tagcagcaaa taatgatact tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc
     6481 tatgtgctta cccaaagcaa ttcaaaaatt tcataactac tattttagga gatgatttta
     6541 tagaatccag agtctacctg aataaaaaac aaacctgtgc atttctagga atcgataata
     6601 atactctaga taagtggatt cgtatatata atttcccagt aataagagtt ggtggtgtag
     6661 ttcgctttga cgtagaagat attaaagact ttatgaatac ttgaaatttt cttctcgccg
     6721 catatactaa aattagtgaa atatactgcc ttactttttt taaggagtga ggattttgcc
     6781 tacaaagctt tcaaatggga aatataaaac taatctgaga tatccaaaaa ggtttaaaga
     6841 aataacagga attgcatcag aaaagtatca aaaaacattt ccaaataggc aattggcgat
     6901 taaagccgaa aatgatatga agaagaaaat agaaaaagtt ttgagagagg agaatgcaaa
     6961 ctcattagaa ttaaaaggaa aaataacgtt taaaaaattt tacgaatcta aatggcttcc
     7021 tagatatgaa ttaggtcaaa caattagatc aaacaggcct cctagtgata taaccattag
     7081 taatacaaaa gatatatttc ggttgcatat tttacctatg tttggtgaat acgcaatgaa
     7141 ttatctaaat atcaatactg agataatatc agatgaatta accaaaaaat cgaaagaata
     7201 tgcaaatata aaaatcataa aaggatatgt tcgttccatg ttcgatattg cagaaatact
     7261 aaattatatc gaatttaatc gaactactaa aattattcaa agtatcactg cacctaaaaa
     7321 aaatgcttta gaagaaaaaa gaatacaaga aggtaagcaa gcactatcat caaaagaact
     7381 tactaattgg atagaagcag taaatgatga ttttaataat catttactta cttttcatga
     7441 ttacacactt tttatgatta ccctgtatct tggtgataga aaaagtgaga cttacgcttt
     7501 gcaatggaaa tatattgatt ttgaaaaaca aacggtccgt cttaaacata ctttagataa
     7561 atatcaaaga aaaaagttta caaaaggtcg aaaagatacc gtcattcaag taccagaagt
     7621 tgtaatgaca ttgctaagtg aatggaaatc ggtacaagct gatcaattgt tgaaattaaa
     7681 aattaagcaa acgttagatc agtatctctt tacttatact aaaccatctg gagaagtaaa
     7741 ttgtcctgtt catgccgatt acctaaacta tcgaatcaat tcaattaaaa gaaggcatcc
     7801 tgacttagtt catttatctc cacacaaact ccgtcatact tatgcaacta tagctagaca
     7861 aggtggagca gatatgaatc aaatttcaaa tgctttaaca cactcagata tttctacaac
     7921 aaagatatat gtgaacacac cagatatagt tgataaggct gtttttgaag cttttcagag
     7981 aggattgaat aagtgtgatt gatatgtgtt cttaaatgtg ttcttaaaaa caaaagaagc
     8041 actttccaaa taattgaaaa gtgccagaaa cgttgatata taaaggatat taacgttttg
     8101 agaattgtga agctttacgt gctttcttaa gacctggttt tttacgttcg accatacgtg
     8161 cgtcacgagt aagtaagcca gcacgtttaa gagctaaacg gaagtcagga tcaacttcta
     8221 gcaatgcacg agcgatacca tgacggatag ctcctgattg tccagcgtat cctccgccgt
     8281 ttacgttaac aaatacgtca tatgcgcctt ttgtttcagt aacagcgaaa ggttgattga
     8341 ttacttcacg caagtcagca tgtggaatgt attcttcaac atctttttta tttacagtaa
     8401 ttttgccagt tcctggtact agacgtacgc gagcaactgc gtttttacga cggcctgtgc
     8461 cgatatattg aacttgtgcc aatgaaattc cctcctatta gattaagttt gtgatgtcta
     8521 aaacttctgg ttgttgtgct gcatgtggat gttctgctcc accgtatacg ttcaattttg
     8581 taaattgttt gcggcctaaa gtgtttttag gaagcatacc ttttacagaa gtttcgatca
     8641 aacgacgaga atttttagca cgcaattcgc cagcagtgat tgatttcaat cctcctgggt
     8701 agttgctgtg acggtaataa attttgtcag tcgctttttt acctgttaat ttcactttgt
     8761 ctgcattgat gacgatgacg aaatcgccag tatccaaatg aggtgtgaaa gttggtttat
     8821 ttttaccacg tagtacagat gcaacaactg ttgaaagacg tcccaatgga acatcagttg
     8881 cgtctactac ataccattta cgttctactt cgcctggttt ggccatatat gttgtacgca
     8941 cgttattttc ctccaattta agttctgatg tttgacatac acttcgagtt tccggggctc
     9001 tttgtggggc aaacaatacc atttaacata ttacccgcta atctagtcaa tgtcaacgtt
     9061 tttctgcttg attgaaaaat ttatatgaaa ctttacttct tttctttctt ttatataaaa
     9121 gggtcgtttc ctgtcaaaat gaaaaacaaa aagaggctgt gacaaaagtc ttgtcacaac
     9181 cccttatttt gaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc
     9241 ataaaacata agaagctgtt ttttaaaatt gtttcttgtg actcggagct gaacggcagg
     9301 cttacaacct cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
     9361 ggcaaaatat gaggaactat tttgacgttt gtttctctaa tactcaggtc aaaacgctga
     9421 tttcacaacc tctttttcgg tcaagttcaa caacagtttt tacgcgtcga tctctggcgc
     9481 tttgcccaaa tgagcttgga tcatccagat acgtttttct gtggctgttt tgaattcgat
     9541 aaagatatct tgtgttggat cgtcgccttc ttctccagaa acttcgattc ctttttggta
     9601 aagatctgct agataacgat agcctgcaac aagtgtttcc aaacgttctg gaatagtacg
     9661 atcccagcgg ccaatttcat cagggatttt tgtgttatct gcaaattcac gcaatgtaga
     9721 ataaggtgaa ccgtctaatg tgatcaagcg ttcggatact tcatctaact gagcgttcaa
     9781 atcatccatg aactcatcca tcattgggtg caatgtaagg aaacctggtc ctctcatgta
     9841 ccaatgtgtt tggtgaacaa caacagacat ttggcttaga tctgcaacta attgatttaa
     9901 tacttctttg gtttgttgga atttcatctt tcttgcctcc taacattttc ttacacttaa
     9961 aataatagta gactttcaat gaaaagtaca acaaaatcat ttacctgaga acgattttca
    10021 gttcgctttt ttatccgtaa agccaatcat ttttcagaat cagctaattc ttctcgcagt
    10081 tgagcaaatt gctgataagt ctgtgctaaa tgcttttgtg tcgaaacaaa taatggatag
    10141 attttctgat attttttatg atcagtttct tgtactccaa cttgctcttt caattctatc
    10201 aattcctgtg cttcactgat actttggatc tttccgatgc ttctccagcc taaaatcaca
    10261 gctcctaatg cggatgcttc tgtgacagta gggaaaacaa gcgggtaacc cagtacatca
    10321 gccatcatct gacgaaaaat cggagattga gcaaacccgc ctgtagcacg gatctctgtg
    10381 agctgatttt cctggcaaac tccttttaaa atccgataaa ggttgagaca gatcccttct
    10441 agaatcgcct tcatcatttc tttttctgta tgttgccgct gtaaaccgat aaaactgcca
    10501 aacgcatctg cgtcccaaag cggtgctcgc tcaccaagta aatagggatg aaaaatcaga
    10561 ccattggcac ccgctggcac aaaagcgata gccgaaagca gtgcatcata tggattttct
    10621 cctttttctt tagcagattg aacgacttcc tgtaaaaatg tatgccctgc ccaatcaaag
    10681 atgccagcac cattgctagt cgctcctccc actacccaat gtttctgatc cagaacataa
    10741 caaaacgtct ccatttctgg atggaactga ggctgagtag taataaaacg aagtgctccg
    10801 ctcgttccta cggtcaaagc ggcgatcgta tcaccaaaag cacctaaccc tagattagaa
    10861 agcggcccgt ctgcccctcc caaaacaaat gatatatcat ttggcaaacc tagttctgct
    10921 gctgtttttg atggtagacc tgttacttga taggtggaag gaaagacttg cggcaattgc
    10981 tctattcgaa tgttcaagta gtcaagcact tcttctgccc attggaattc atggatatca
    11041 aaatatccag ttcctgaagc tgttccgtag tcgctgatca gttcgccaaa caatctataa
    11101 aagatatagt ctttgatacc gacaaaataa gcagtttttt tgaataattc aggttggctg
    11161 ttttgcagcc acttgatttt gttcaatggt gtcattggat gaatcggcgt cccagtctgc
    11221 cgataaaatt ctttgccaag cacggtatat ttcagttttt ctgcttcgtc cgctgctcgg
    11281 ttatctgccc aagtgatcat tcgagataac ggtgttttct cttcatccaa caatatcagg
    11341 ccatgcatcg cagaggagaa ggagacaaat tgaatgattg tgtctgaagg caactgttta
    11401 attacttctt ttataacggt gcagacacct tgaaagatca attcctgatc ttgttctgcc
    11461 atcccttgtg tttcttggat caaagggtag cgaaaataag cggatgctgc ttcttgggct
    11521 tcatctgtga aagcaactgc tttggtttgt gtggttccga tatctaatcc gattgaaacg
    11581 ttcatttttt cacctcaaat tttatgtatg tacaaaaatt tagtttaagc ttgcgtatct
    11641 tggttttgaa aagggggaga tggcgtgtta ctttatttta tcattggttg ctgtatcgga
    11701 tcatttttat gtttaaccgc ccagcggatt ccgttaggtc actctattat ttatcctaga
    11761 tctcattgtg tcaaatgctg tcgttcgctt tcttggtatg aattgattcc gattctctcg
    11821 atcatcgtcc aacgatttcg ctgccgctat tgcaggtgtc ggttgccctt ctactatttg
    11881 ttggcagaag ccttatgtgg tggtcttttt gcctggttct ttactttttc ttccgcaaga
    11941 aatttttcga cgttcatatg gatgctttcg gctttgctat tttcgttgat ggatttattt
    12001 tacttcatgg tccattctca cacgctattc tgttcttggg caattttatg gattttttgg
    12061 ttgcaggccg gcgtcttcca atggcaaagt gtgctgttaa cgtttatcgt aggcggtgtg
    12121 tgccttcgct atggtcaatc ctatctcgga gctggcgata ctctgttgat gctttcttgg
    12181 agtggtggct tgtctctaga agagttgctt cagatcttgt ttcttgctag tctgtttgga
    12241 atatccgttt tttctgttta ctttatctgt cataaaaaaa agttggaaaa gctgcctttc
    12301 attccttttt tgagcagtgc gttgctgatt gtccttcatc ttaggtattg aaaaaaggtt
    12361 gtgacaaacg acttgtcaca acctctgata tatagtgttc tcttaccaaa tccttgtgac
    12421 ttggagctag gcgacaggtt cacaacctct ccactaaatg gtgttcaatc agctgaccct
    12481 cagtattagc tcaaaatgct ggttttacaa cctctgatat acggtgttcc ataagtagct
    12541 ttttcggctt cttatttctt gacgccccaa tgtcgacgaa ggaaacctgc acgtccagaa
    12601 ccttctcgat aaagattgta atggagcttg tttttcttgt aataatcctg atggtaatct
    12661 tctgcaggat aaaattcgct tcttggttcg atttttgtca cgattggctt atcgaatcgc
    12721 ccgctgtctt gcaatgcttg ttttgaacgc tctgcaatat ttcgttcttc ttcatcatta
    12781 tagtagatca ctggacgata agaatcaccg cgatcagcga actgtcctaa tgcgtcagtc
    12841 ggatcggttt gctgccaata gatttccacc aactgatcat aagaaataac ttctggatca
    12901 tattcgatct ctactgcttc tgtatgccct gttgtgcctg ttgttacttg ttcataagtt
    12961 ggatttggta catgcccgcc agtataaccg gatgtcacag aaatgatccc tggctgctct
    13021 tcaaatggtt tgaccataca ccaaaagcat ccgcctgcga aaactgcttt ttttgtcata
    13081 taaaatcccc cttttttctc atcatacctt gatttccaaa aggaggataa cgttttgctt
    13141 gtgacagaaa aaacggaaac tattatatac gtgatacggc ttctgataaa tgccaaaacg
    13201 aacgtacttg atcaaaaacg gttaaatcag gaatcacata ttcagcagaa ggaagattta
    13261 tgttttcacc tattcccaat acatgcatgt taccagccaa agctgcttga caaccagctt
    13321 ctgaatcttc aatgaccaag catgcatttg gcggtacatt caattgctgt gctcctttga
    13381 gaaaaacttc tggatcaggt tttgcttttg atacttgcgt gccgtcaata aggacatcaa
    13441 aataacttgt caaattcgtt ttttctaaaa tgaggcgtgc atttttgctt gctgatccca
    13501 atccgatctt gatgtttgtt tttttcaagt actcaagaac agctattgca ccaggcaaaa
    13561 cggatgtttc atccattttt tgaatcgctt gaagatatag attgtttttt tgctcagcaa
    13621 aggctgattt ctctttttct gagaatttct gttccatttt tccaaatgac agcaatcgct
    13681 ctagtgactc ggttctggat attcctttca gttgctcgtt ttctttctca gtgaatggaa
    13741 tggacagctg gttagctaaa accagccacg cttcgtaatg ataacgagct gtgtctacaa
    13801 gaacgccatc taaatcgaat agtgctgctt tgattttcat cgattacctc cttgaaaatg
    13861 aacgacttga agtaaagatg cgatgttttt agctgtgcga tgaagattat cagtggcttg
    13921 ctgtaaagct tcttgtaaag gcacagctgc aggaaggatc ggaaaaacgg ccgaaatgcc
    13981 aacatctgct gctttttcgc ttcctttacc aacacttccg caaatagcta tcacaggaac
    14041 gccctctggt actcttctag caatcccaac tggcgctttt ccttttagac tttgtccatc
    14101 tagacgtcct tcgcctacga ttacgaggtc tgcttgctgt acccgtttat cgaactccag
    14161 taaatccaat acgtagtcga tgccagagac tattttccct gaagcaaatg ctaacagtcc
    14221 tgcacccatt ccaccaccag cactactgcc cgatgcctcg attacttgag gggcaaatct
    14281 catataaaaa tcatgcatgg cttggtcagc cactgctaat tcattttctt tcaagccttt
    14341 ttgtggtccg aaaacaaaag aagcgccttc ttttccgcat aaaagattag taacatcagt
    14401 aatgacagtg attttggctt taatcagctc aataggcacg ttcgttttat caactgtagc
    14461 aattttacct agattttctc ccagtgcttc tactttttga ttatcttgat cgtaaaaagc
    14521 ataacctaga ccttctgcca tgccgatgcc gccatcgttg gtactacttc cgccaacgcc
    14581 gatgatgatg tgtcgaacac cttgttgcaa caattgtcgt atcagttctc ctactcctcg
    14641 cgtggataac ctaagtggct gttgttggtt tagtgggaca tctttcaatc cgacgatttc
    14701 tgccatttca aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc
    14761 aaatgcatta gtaacgactg ctgtttgccg cttcaacctt aaactgtctg ctaatgatga
    14821 aaccgttcct tctcctccat cgcctatagg tagcagatca tattcagcct gaacaaaaac
    14881 ttcttgaaaa cctgattgaa tggcttgtgc tgcctctaag gctgtcagac tttctttgaa
    14941 agaatctggc gcaatcagaa tacgcattat tttcccctcc gttccaagat cagttctttt
    15001 tccagctggt attggtctcc attgacaaaa ataggtaatg tttctttcgt ctcttttttt
    15061 attactattt gtttttttgt caaaacaaat agcaacctgt gatttttcca aataaaagga
    15121 aaagaaattt tttcccatgc tgaaggaagt cttggagaaa tagaaagagt tccattcgtt
    15181 actgtaatac ctgaaaaacc aaatataact gctagccata gtgctcctaa tgaagcagca
    15241 tgaattccgt catctgatga atgcggatct ggtcctaaat caatcaggca cgcttcttgg
    15301 aataaatgat acgcccattc catttcttgg atacggcaag cctctatggc gtgaatcgct
    15361 ttactcaacg atgagtcgtg gattgttttg ttttcatagt aatgcaaatt cttagaaacg
    15421 attggttgag aaaaatactc aggtaataag tataacagca tgacaagatc tgcttgcttg
    15481 ctgacttgga gttcatttac ttcgctacgg gcataatcaa gtaaaataga ctggctgcca
    15541 ggacgttctt tgtaagtggt caaatcaata actggcttac tgagatacgt gtcatcttgc
    15601 ggaatcagct gttctttgtt aggcactggc aaatataact tatctaaaaa attttgacat
    15661 tctttcaaaa aaagcggatc gccagcttga tatttaacag ctaaggccac attataatgc
    15721 gccagatagt tcgtatatgc attattgtcg atatattctg tgtattcatc tggaccaatg
    15781 actctgtgta tctctaaatg atcatcgact tttacagctc tgctcagcca aaaagctgct
    15841 gtttcatgaa tgagcgtttt tccctttgtt tctaaaaacg attgatcctc tgttgcttgg
    15901 acatactctt gaacggcata agcaaggtct gcactgatat gatgttctgc ggcagctgaa
    15961 gcaacttctt gtcttgttcc ggttttgata ttcaatgcag caaatttggg tgtctcttcc
    16021 tgaccagtgt gtgcgctttc ccatgggaaa agtgcacctt gatagtgata atctcttgct
    16081 ttttgccgtg cgccttctaa tcgatcataa cggtacagca gcaaattctt tgctacttca
    16141 ggaaactgat ataaaaagaa aggcaggata aaaatttccg tatcccagaa aacatgccct
    16201 ttataacctt cgccagttag tcccttcgcc cctacactaa cacttggatc attgcgggga
    16261 gtcataatct gtaaatggta agcagcgaag tccatcgcta actgatcgaa agaagaatcg
    16321 ccttggatcg ttatccgatg ctgatgccaa aaatctgtcc aataagacat cgactgctct
    16381 aaaatttctg tataactaac gcttttctgg gcttgggcaa tacttttctt aaacgggtct
    16441 gtttgctgaa gagaggtaga gagcacgatg atcttctcca gcgtaaagga cgtatttttt
    16501 ctgatctttt ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa tacacctgct
    16561 ttattgaatt tagctgcaat cccaatatca attttacttt ctgttgtctg gtaggatgca
    16621 ctgatcaaat cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa
    16681 ttggtttgct gtccatcgat acccgttttc acatctactt ggcagtcttc ggtcaacggc
    16741 ttgatgacca ctttcagggc tgcctgatgg agattatttt gatcagcaac acgacaagta
    16801 atgacttcca ctttttttcc ttgagcagtt tcaatgagcg catagcgttt aagctctccc
    16861 atttctaaat ctagtgcccg ctcgtatgca agaactttca tcgtctcaag agaaaaatgt
    16921 tcaccgtcga tttttatttc ccatctgatg acatctggga gattcactaa ctcggctgat
    16981 tctgctccta aaggtcggtt atatacacca gctgcaaaca ttccccttac ttgattccga
    17041 tagtcttcct cctggcttcc tctgatacca aaaaatccat ttccaatggt catctgactc
    17101 gcatacttat tgatatactc taaatcaaac tgtttttctt ctactttcat aaggtaataa
    17161 cttctcctgt ttctgccgaa cgatataaat tttccaccag attctggatg acatatcctt
    17221 gccagccatc tgcaatcatc tgagtctggt caccgagcgc ccgattcaca aaatgagcca
    17281 tacttagttc atgtgggtca ttaactggcg tggtcttttg taaggaaacc agctttccat
    17341 ctacatcttc gaatagttcg cctggataca gattagcacc tgcctgatcg ccacaaaaag
    17401 ccacattcag tttgtcttct ctgatattca aagcaaacga agtattcagt tggatgatct
    17461 tgccgttttc taattccagt gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat
    17521 cccatttccc gaatttccct gaattttttt tcgtaccaat tttttggaag ctgtacgccc
    17581 gaacgctttt tactttagga aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc
    17641 cataatcgat caaaggcccg cctccttgaa gttctttgtc gataaacgtt ccccagcctg
    17701 gaacaccaga ccgacgaagc gcattcgctt ctacgtaata aatatccccg agaacatcaa
    17761 gatgattctt gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa
    17821 tctgcccgtt tttatcggct gtctctgcca tctttcttgc ttgatcagct gtcatagctg
    17881 gtggtttctc gcacatcact gaagcattag cttcgagggc agccaataca tgttcgaagt
    17941 gaaagcgatt gggtgtacag acactcacga tatcgggaga aatctcattc agcatttgtg
    18001 taactgatgt atacgcatga gaaaatcctg tacgtcggca aaagtcttct gctcgctggc
    18061 tgactggatc aaccacagcg gccaattcta agacatctga tcttttttga taataagcag
    18121 catgtacttt ttctgctact tgtccagcgc ctatgattgc tactttcttc atttatttct
    18181 ccctcctttg cttatttact tattgttatt ctttctctat tctttctcta aatcatttaa
    18241 atgacgcgct gtttccagac agtttttcaa gtattgcaat gattcctgat aaattttcac
    18301 ttcattttct cctcgcaaac gacattcata taccatataa ccttcatact cattttgaag
    18361 tagcgtttga aaatggcgag taaaatcaat tgcgccagat cctggttggt agcgatgatt
    18421 atctgctaaa tggatatgac ccacatactc tttttgcgta gtcaatgctt ttgaaatatc
    18481 atcttcttca atattcatat gataaaaatc agcgatgatc tgcacatgtt tcaattgatt
    18541 agagtcaatg taatgacgcg catccgctaa caaattgatc atatggtctt ggtatcggtt
    18601 caaaggctcc aaataaataa ccgtacctgt ttcttcggct actttatcta aataacgaag
    18661 tgattcgctg actgctttat agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa
    18721 tcgataagtg aacattcccc aagctgctgg gataacgatt cctttgcctc caacctcgct
    18781 tagtgcacgc aaaatctcag caatttgacg tacaccatta agacgccgtt cttcgataaa
    18841 gtcaccgatc cacccatcgt agccgttaca agctgtcact actgggatcc ctgtttcttt
    18901 gattgcctgt ttgactgtgt cgatattttc tattaacaac ttaccgtcga tttcgtattc
    18961 ttcaaatcca cagtctttga taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc
    19021 ttgatcttgc gtaccgaatt tcattgttct cctcctcgga cttgaacgcc cattttgata
    19081 ctttcttctg ggtgctggtc tacataacgc atgtaagctt ctgcgctttc tgcaaatggc
    19141 acaactggat caatgatttc ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat
    19201 gtgtcctcga ttcgtttacg gttccatctt gggtaatctg ggtttggttc actagatgct
    19261 cgagcaaaga cgatttttgc attattgaaa tgtgcttcgc gaccaagatt aaacccagct
    19321 ggaaaaggtt tagcaaaggc aacataagaa atgatcccgc catagccgat ccctcttaaa
    19381 gaagcttgta atgcggcagc actgccactc gtttcaataa ctgaatctac tccacgttta
    19441 tctgtcaatt cctttagacg aaaaccgaca tctgctttca taggatctag cgtgtaatcg
    19501 gcaccgtatt tttctgctaa ttctctacgc tgcgctatgg gatcgatccc aacgatggta
    19561 gtagcacctg cttttttagc taattgaatc gcaatttgcc cgatagcgcc taaaccgaag
    19621 attgctacag catcacctgc tcgaacatgt gcttcccgga taccacctaa agcgaattga
    19681 gcaggatcgt aacatactgc atttttccat tgatccaacc gatcaagttt acgtagttta
    19741 taattatcaa caccgtttgt aatcagcgtt tcacgaatgg gtccataagt gcagactaac
    19801 tctccttttt gatactctgt caccaaactg cccgtttctt cgatcgtacc gacaatcata
    19861 tttcctaatt gaaattcgcc aaataccaca ccgcgttggc ttccttcttc tcttggcaag
    19921 aataggcgcc attcttctga aaattcttca tcgataaatg gacttaatcc tctaaagtcg
    19981 acaatttctg ttcctggttt tggagtagcg aaaattactc ggatacgtac ttcgttcttt
    20041 tcgatctcac ggtcttcgta atctttgaat ccggctattc ttggttctaa tgcaactaat
    20101 tttttcatct tttctttcct ctttcttttc tttttatcct tttacgccgc cttcgactaa
    20161 atcaccttta ataaatcgtt cagataacgc atacataatg atgactggta aagcggtcac
    20221 caatgaagcg gccatcattc gtccccaaat gtagtctggt gtactgaata atgtgttcaa
    20281 accgattggt aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa ataaataatc
    20341 attccacgaa accataaagc aataaacgaa gacagaaatg attccagaaa tagccaatgg
    20401 tataatgatc cgaataatga tctggatccg gctaagacca tcgatcattg ctgcttcttc
    20461 aatatcagtc ggaatcgtgt caaaataact ttttagcatg aatatcgcag taggaagtgt
    20521 ttgaacgacc atcgtgataa tcagtgctgt attggtatca tacaaaccta gaccggaaat
    20581 gattttaaat aatggaacaa cgatcaaaat accggaaaac atataaacgg tgtaaaagct
    20641 agcattgatt ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg ccccgctgat
    20701 cccaatcact actgcagtta cggaggctat caaggagatc actaaactat ttttgaaata
    20761 catgatgaat ggaaaaatct cagcattgaa aatatcctga taatgttcaa aagtccattc
    20821 tttcggaaaa atagtttgtg tcgttgcaat tgcttcttgt gaatccttga atgatgtcat
    20881 cagcatgatc aaaaatggaa acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa
    20941 taagattttc ctgctctttt tatttttcat ttcaagtcca ccttctttcg tgagagcaag
    21001 acgaccgctg caataatgat gaacaataga atcgatatag ctgctgcttt tccaagatca
    21061 ttgaacgcaa aggctgtctg atacaaataa acgcctaaga tattgacttt attcgtcagt
    21121 agatagacat ctgtaaacat atagaacatc cagatagatc gtaaaacaat cacagttgct
    21181 agtactggtt tgattgccgg cagtgtcacg atcttgaact tctgccaagc atttgcaccg
    21241 tccatttcag ctgcttcata aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa
    21301 ataaacgcat acggatagta tctccagata gcaaacaaaa cgaccataaa gaagctagca
    21361 actggctggt cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc tactaacaga
    21421 tcattgacga ttccataact attattgaac atatatttcc aagcaaaaat caaagaaata
    21481 gatggcgtaa cataacttaa taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg
    21541 cggttaagca aaatagctac ggctaatcct aaagctgtac tgcctgctac aactaagaat
    21601 gtgtaagcaa ctgttaaacc taaagaacga taaaattcac tatcacttaa aatttcaaga
    21661 taattgctta atccgataaa aacggcgtct gagttaggat tcagcggcat atcgaaaaaa
    21721 ctgatctgaa tattggaaag cattggaatc agcactaaaa cagctaataa aagcatgctt
    21781 ggtgctaaaa gtcccatggc taattttaag tcagaatgtt tccttggacg attcattgaa
    21841 acctccccct gataatctgc caatagctat ctactttttt cgaacaccat tggcagaact
    21901 tcttgttatt tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg
    21961 acagttactt ggttcactgc ttttccgatc acacctgagc ttgtgatatc tcccattttc
    22021 gtgaaattct tttccccgac tagtccaaag acttgtactt catcaaaagc actagcaatc
    22081 tcagatggca gctcgccaaa agacttgatg acttcatttt ctttgtaacc atccagttcg
    22141 actacttgtt tattcactgg ttgagcacca ccaggagaca tcaagaccca cttttccatg
    22201 tttttcggtt gagataagaa agctgtgaat gcttcagcag cctctttttg tttctcgtct
    22261 agaccagaag aaatcgtcaa tccagatact gtgccataga cagcttgatc tttttgtgtt
    22321 gggatagcaa aaccgatatt ttctgagttc cccgcttcat aaacagaagg caaaatgtaa
    22381 gttgaataga ttgccattgg tgctgttcca ttcatgaaag catctttgat ttctgtcaca
    22441 tcattagagc ctggcatcgt gtattgggat aagttttgat aaaacgacaa tgcttctctc
    22501 atcttttccg tatcgatcgt tacctcgcct tcatcatcca aaacatttgc tccatttgac
    22561 aaagcaaatt gagagaacgc ttgctcactc attgttcctt cagcagtagg catagcaatt
    22621 ccatattgtt tattctcttt atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa
    22681 ttttctggtt ctgaaaaacc agcttctgat aatttttctt tgtcatacca aataccttga
    22741 acccagccgc taattggtgc agcaatatag ctttttccat cttctgaacg aacaaggttt
    22801 ttggctccgt cataataatt atcttctcca gcttcctcga taacgttttg gactgccgtc
    22861 tgatcaatca gttcatcttt atccatcact ttagcaaaat cctgactgac ttccattact
    22921 tctggtaatt taccggaacg agctaacgta acgatttttg tattgaacgc tgattcttct
    22981 accggcactt gcttgatttt gatgtctgga ttttcttttt caaaatcagc aaccagttca
    23041 ttgatcacac ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt
    23101 tgtgattcgt tagagctttg actttcacca caagcagtaa gaagaaatcc agtagctagc
    23161 agacaggtag taacaataat ttttttcatt tttttccacc tcttatttta ttattttcag
    23221 ccacatatat tcatacggct ggaatatcaa ctttcgttct tgactgcaat ggataatttg
    23281 tccagaaaac aggtctaggt aatctccttt tggtaaaaga taacctgctg attgattgga
    23341 aaaattatgg ataactaaga ctttctcttc cttcgcttcg cgaataaaac tgataaaaga
    23401 atctgctgag tccagtaagt aaatcaaact attcggatgg aaggcttttt ctttttttcg
    23461 aatagatagc atctgggtca attcaccata tactgctgtt cttaatggat ctgtctgcaa
    23521 ctctttctca atatctgcta acaaaaattt ttgccgattg atttttcgat tctctcctgt
    23581 tttttctacg cctttgtaat catttcgact tcccagcaca ctttgaatat aaattgccgg
    23641 cacgcctggt aacgttacca aaaatgaatg agcagctaaa aagcgtttgc ttctccattc
    23701 cgtcgtatct gttggagaac ttaacgcatc tagatacgta gcgtttatct catatggact
    23761 ggtcgttcca tctgggtttt tcttgtagtt tactcgggta ccctctttct tcagctgttc
    23821 cactaattca aggatcgttt tttccggcag aatacctcgt accggattca atccgatacc
    23881 gtcatgggag gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt
    23941 taaccattct ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg
    24001 tagcgaaaac tgatagacca tctgtgcttc attctcgcca ttcccaaaat aactgatatt
    24061 atctttgtgt ggcacatttg tctcagtgat caataccgtt cctggagctg cttcatcagc
    24121 gattgcacgg aataattgga tgattttatg cgtttctggc aggtgtatac aagaagttcc
    24181 tagttctttc cataaaaatc ccacagcgtc caagcgaata aatgaagcgc ctttttctaa
    24241 gtaaaacaaa agaatatcga tcattttcaa taaaacttct ggatttttaa aattcaagtc
    24301 tacttgatct tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa
    24361 cgttgtaagt aacgggctgg tccttggtct agtcacgctg cttgtatctg tatccagtgg
    24421 caaatcaata aaaaaatctt tgtacgtatc attcaagttt aaatattcct gaaaccaagt
    24481 actttcggca gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat
    24541 ttcttctata tcttcccatg tgcccaaacg gtcgttgatt gcctgataat ttacaacgga
    24601 gaaaccatca tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc
    24661 tgccaaatac ttttgataaa atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa
    24721 aaattgatcc ccataagtaa tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc
    24781 ctggcctttc ttctgctgat aaacactgac tttttgttca atcatagagg cggttttctc
    24841 agacaaatct gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg gattcccctt
    24901 tcttttttgg gagcgttccc aacgatttga tcatattatg gaaaacgctt actgtcaata
    24961 gcattttttt aaataatttt gatatacaca aaaatacaaa taagaaagct tatgtatcaa
    25021 taattaatag cttcttaaaa aaatatggtc tctcatgatc atgcaagcta ttcctaaatt
    25081 ttcaaaatca tagacaaaaa agaatgtcta ttgtactatg gaattattct aaaacgctcc
    25141 caaaaaggag gtgcatataa aattgaaacc tactatttat gacattgcga aaaaagctaa
    25201 tgtctcaaaa tcaactgtct ccagagtatt aaataaccag ccaaatatct ctgataaagc
    25261 aaaagcaagt gtacttcaag caatcaaaga attgaattat gaaccgagca aaattgcaag
    25321 aggactgtca ggaaatggtt ttgacgcgat tctagcatta tctcatcgta cttctcatac
    25381 gacctatggc aatccttttt tttcagatgt tattcaatcc atttctgccg tttctgaaca
    25441 aaataacttc gacttgattc tacaaacggc aaaaacacca gaagaagagc tagaaaaatg
    25501 cgcgaaaaag atccaagaaa aaatgatccg aggaattatt attttaagct ctacgatgga
    25561 tgaaacactt ctttttgaat tggatcaata caatattcct attgtggtaa tcggaaaagt
    25621 cggaaacgac tacacgaaca tctactcagt ggacacggat aatttccagg atagttacga
    25681 tttggtggaa acactgatca agaatggaca ccgtcatatc ggctgtctcc atgctccgac
    25741 agaagtttat gtatccaatg atcgtctcaa tggttaccgt caatgcttat ttgatcacca
    25801 cctagatatc cgtaatgaat ggattataga cggcggcttt cgcttagaag atagcatggc
    25861 agcaatgaat gatttaatga agctacgaga aagaccaact gcgatttttt ctaccgatgc
    25921 attaaaatca ttgagtattt acaaagtggc agaacaatct ggaaagcaga tacctgatga
    25981 tctttcgatc gcaggattca gtgatccctt attgtcctcc ctattttcgc cacagttgac
    26041 tcggatagac attccgacaa atcagttagg aatcaaagcc acagaacttt tattccaact
    26101 aatccaccac caataccctg aagaaaaaag aatcattctt tcaaccgaga ttactctcac
    26161 agattcgatc caacaacaag aaatgtagaa cagaattata aaagaacaga aacactgagg
    26221 ataagtcagt tactctgttc ttttttcaat cactcatccc tcgttacccc ctcccggtat
    26281 tttagcgaat ttttcttatt ttccatctgt tttagatagc tgtggataac tataatgttt
    26341 gtgttaaact attttttcca cagggaagaa aaaactattt ttaaccacgt tgtgtataag
    26401 tttttatagt tcgttttttt aaacacattt acccttattt caatccatgt tatttgggga
    26461 tacttatctc taaaacataa atagaataac agacatgtct actggttttg ttttattcga
    26521 ggaattattc aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat
    26581 ttattttgca ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga
    26641 tgatcgttcc gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt
    26701 tcttctagtt agacaaatta ttttagacaa accaaaaaag aggttgtgac aataatttgt
    26761 cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca
    26821 tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg
    26881 gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta cctataataa
    26941 cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga
    27001 cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt
    27061 ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat
    27121 tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat
    27181 cacctggatc cattacacga actttacgca gcatttgacg aaccattact tcgatatgct
    27241 tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt
    27301 tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg
    27361 tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag
    27421 gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg
    27481 caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt
    27541 tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc
    27601 cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg
    27661 attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt
    27721 tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga
    27781 ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt
    27841 ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac
    27901 gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga
    27961 tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt
    28021 caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc
    28081 ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt
    28141 tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc
    28201 cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga
    28261 ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat
    28321 tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt
    28381 gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca
    28441 gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta
    28501 agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt ttgaagactt
    28561 ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga
    28621 tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca
    28681 agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga
    28741 tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt
    28801 cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc
    28861 atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt
    28921 cttccattgt caagtagtag ttacctagaa ccatatcttg ggaaggagta acaactggtt
    28981 taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt
    29041 gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat
    29101 aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga
    29161 atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct
    29221 tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg
    29281 catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga
    29341 ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa
    29401 caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac
    29461 gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag
    29521 taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac
    29581 gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt
    29641 tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac
    29701 cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca
    29761 tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg
    29821 cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg
    29881 catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga
    29941 attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt
    30001 tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt
    30061 ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt
    30121 accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt
    30181 tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt
    30241 tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca
    30301 acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac
    30361 cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt
    30421 tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc
    30481 tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt
    30541 caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta
    30601 attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt
    30661 cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca
    30721 tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg
    30781 gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt
    30841 catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg
    30901 cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt
    30961 gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt
    31021 gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg
    31081 tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt
    31141 cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact
    31201 gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca
    31261 cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt
    31321 cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc
    31381 cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg
    31441 acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc
    31501 catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat
    31561 gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa
    31621 gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt
    31681 ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg
    31741 tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta
    31801 ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac
    31861 caactaaaac gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca
    31921 gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg
    31981 ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc
    32041 ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt ttacataaca
    32101 aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt
    32161 gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg
    32221 agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg
    32281 atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag
    32341 ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta
    32401 cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg
    32461 tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat
    32521 aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca
    32581 tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca
    32641 atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca agagggtttg
    32701 tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa
    32761 ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc
    32821 tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt
    32881 caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac
    32941 cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt
    33001 ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg
    33061 gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg
    33121 gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt
    33181 ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg
    33241 tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt
    33301 aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt
    33361 tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca
    33421 aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc
    33481 gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg
    33541 cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt
    33601 cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga
    33661 atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta
    33721 actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta
    33781 atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca
    33841 aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta
    33901 ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga ttgccattga
    33961 agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt
    34021 ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg
    34081 cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca
    34141 cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat
    34201 attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat
    34261 agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc
    34321 cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata
    34381 tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa
    34441 cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga
    34501 cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc
    34561 aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag
    34621 gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa
    34681 agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa
    34741 aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc
    34801 catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa
    34861 tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg
    34921 cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac
    34981 ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag
    35041 aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag
    35101 acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa
    35161 tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca
    35221 cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca
    35281 atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata
    35341 tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga
    35401 tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga
    35461 cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg
    35521 gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg
    35581 caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc
    35641 tagcaacagt aggaatcgac tacgaaagct tgggtaaaca aacagggatc atggcagcaa
    35701 aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac
    35761 tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag
    35821 ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc
    35881 atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt
    35941 ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt
    36001 aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt tagggggtgc
    36061 ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc
    36121 agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa
    36181 catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa
    36241 agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt
    36301 tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat
    36361 gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa
    36421 tggatgcatt gctctttctg gctccttgct tgcgcaaaac aacggctttg ctgatttaaa
    36481 ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt
    36541 acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg
    36601 ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc
    36661 tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc
    36721 tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat
    36781 catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc
    36841 ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg
    36901 ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa
    36961 aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat
    37021 cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa
    37081 cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa
    37141 gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg
    37201 ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa
    37261 ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg
    37321 gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac
    37381 atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc
    37441 gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc
    37501 aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa
    37561 agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt
    37621 tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg
    37681 atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta
    37741 ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc
    37801 cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac
    37861 gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa
    37921 gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc tgaatctacg
    37981 atgaaagatg tcaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct
    38041 gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga
    38101 ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat
    38161 acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct
    38221 tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct
    38281 gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt
    38341 aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc
    38401 tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa
    38461 ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc
    38521 gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa
    38581 gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct
    38641 tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga
    38701 cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc tccttattct
    38761 atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat
    38821 ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc
    38881 aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa
    38941 aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat
    39001 ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat
    39061 caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta
    39121 cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc
    39181 ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca
    39241 gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt
    39301 cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc
    39361 gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg
    39421 gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg
    39481 cccgatccac actctcgcat agcatatcta aataatccat ttattttcct cctttttatt
    39541 tatcctactt actttttcct aattctagga tacctactta tatgttacta cacaccctta
    39601 ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt
    39661 gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat
    39721 aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa
    39781 tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg
    39841 ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta
    39901 aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta
    39961 gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata
    40021 tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat
    40081 tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac
    40141 ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc
    40201 aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt
    40261 ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg
    40321 tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc
    40381 cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg
    40441 taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg
    40501 atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa
    40561 tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt
    40621 ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc
    40681 ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa
    40741 atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca
    40801 atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct
    40861 cagattcatc aacttcaagc gacagtacag atactacctc taccacaagt gatagcacgg
    40921 atacatcagc ttcaagcgac agtacagata ctacctctac cacaagtga
//
LOCUS       DAZKOB010000014        40145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000014 DAZKOB010000000
VERSION     DAZKOB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.014"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..889
                     /locus_tag="ACG8M6_000768"
     CDS             <1..889
                     /locus_tag="ACG8M6_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0456458.1"
                     /translation="TTGEDGKLAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQ
                     TAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLA
                     IDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKID
                     DQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELD
                     ATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILS
                     ILGVLLVFISIKFRKKRNI"
     gene            complement(1242..2090)
                     /locus_tag="ACG8M6_000769"
                     /pseudo
     CDS             complement(1242..2090)
                     /locus_tag="ACG8M6_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2243..3355
                     /locus_tag="ACG8M6_000770"
     CDS             2243..3355
                     /locus_tag="ACG8M6_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0456459.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3433..3726)
                     /locus_tag="ACG8M6_000771"
     CDS             complement(3433..3726)
                     /locus_tag="ACG8M6_000771"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0456460.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3894..4208
                     /locus_tag="ACG8M6_000772"
     CDS             3894..4208
                     /locus_tag="ACG8M6_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0456461.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4229..6208
                     /locus_tag="ACG8M6_000773"
     CDS             4229..6208
                     /locus_tag="ACG8M6_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0456462.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6221..6547
                     /locus_tag="ACG8M6_000774"
     CDS             6221..6547
                     /locus_tag="ACG8M6_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0456463.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6563..7081
                     /locus_tag="ACG8M6_000775"
     CDS             6563..7081
                     /locus_tag="ACG8M6_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456464.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7081..8433
                     /gene="celB"
                     /locus_tag="ACG8M6_000776"
     CDS             7081..8433
                     /gene="celB"
                     /locus_tag="ACG8M6_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0456465.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8517..10022)
                     /locus_tag="ACG8M6_000777"
     CDS             complement(8517..10022)
                     /locus_tag="ACG8M6_000777"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0456466.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10114..10704)
                     /locus_tag="ACG8M6_000778"
     CDS             complement(10114..10704)
                     /locus_tag="ACG8M6_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0456467.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10883..12529
                     /locus_tag="ACG8M6_000779"
     CDS             10883..12529
                     /locus_tag="ACG8M6_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0456468.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12578..13297
                     /locus_tag="ACG8M6_000780"
     CDS             12578..13297
                     /locus_tag="ACG8M6_000780"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0456469.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13776..14723
                     /locus_tag="ACG8M6_000781"
     CDS             13776..14723
                     /locus_tag="ACG8M6_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456470.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14735..15655
                     /locus_tag="ACG8M6_000782"
     CDS             14735..15655
                     /locus_tag="ACG8M6_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456471.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15696..17153
                     /locus_tag="ACG8M6_000783"
     CDS             15696..17153
                     /locus_tag="ACG8M6_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0456472.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17498..18112
                     /locus_tag="ACG8M6_000784"
     CDS             17498..18112
                     /locus_tag="ACG8M6_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0456473.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18105..19829
                     /locus_tag="ACG8M6_000785"
     CDS             18105..19829
                     /locus_tag="ACG8M6_000785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0456474.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19826..21277
                     /locus_tag="ACG8M6_000786"
     CDS             19826..21277
                     /locus_tag="ACG8M6_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0456475.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21750..23903)
                     /locus_tag="ACG8M6_000787"
     CDS             complement(21750..23903)
                     /locus_tag="ACG8M6_000787"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0456476.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23905..24951)
                     /locus_tag="ACG8M6_000788"
     CDS             complement(23905..24951)
                     /locus_tag="ACG8M6_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0456477.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25101..26390
                     /locus_tag="ACG8M6_000789"
     CDS             25101..26390
                     /locus_tag="ACG8M6_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0456478.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26403..29105
                     /locus_tag="ACG8M6_000790"
     CDS             26403..29105
                     /locus_tag="ACG8M6_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0456479.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29256..30215
                     /locus_tag="ACG8M6_000791"
     CDS             29256..30215
                     /locus_tag="ACG8M6_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0456480.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            30480..30959
                     /locus_tag="ACG8M6_000792"
     CDS             30480..30959
                     /locus_tag="ACG8M6_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0456481.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            30992..33220
                     /locus_tag="ACG8M6_000793"
     CDS             30992..33220
                     /locus_tag="ACG8M6_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0456482.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            33349..34992
                     /locus_tag="ACG8M6_000794"
     CDS             33349..34992
                     /locus_tag="ACG8M6_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0456483.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(35065..35610)
                     /locus_tag="ACG8M6_000795"
     CDS             complement(35065..35610)
                     /locus_tag="ACG8M6_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0456484.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(35633..36118)
                     /locus_tag="ACG8M6_000796"
     CDS             complement(35633..36118)
                     /locus_tag="ACG8M6_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0456485.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(36108..36920)
                     /locus_tag="ACG8M6_000797"
     CDS             complement(36108..36920)
                     /locus_tag="ACG8M6_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0456486.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(37059..38591)
                     /locus_tag="ACG8M6_000798"
     CDS             complement(37059..38591)
                     /locus_tag="ACG8M6_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0456487.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(38700..39731)
                     /locus_tag="ACG8M6_000799"
     CDS             complement(38700..39731)
                     /locus_tag="ACG8M6_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0456488.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT        13163 a         7063 c         8022 g        11897 t
ORIGIN      
        1 aactacagga gaagatggaa aactggcaat tgacggttta gcaccaggag catatcagct
       61 ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg agtttgagat
      121 cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta caccaggcgg
      181 tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag ctgtttttga
      241 gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag cagatggaaa
      301 actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc aagcgccaac
      361 ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc agacagcggt
      421 ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga caaagatcga
      481 tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      541 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag atggcttagc
      601 accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat tggatgcgac
      661 accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa caaaagagaa
      721 tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca gtggaaataa
      781 gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa tattgggtgt
      841 gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag ctaaaataaa
      901 aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat ggaaactacc
      961 gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt tgaacccaac
     1021 cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa acctttgtag
     1081 taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg agtttcgcag
     1141 tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt tctacaaata
     1201 atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa taaggagaaa
     1261 aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa ggttacgcct
     1321 ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca tttggtagaa
     1381 catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat tctatttctt
     1441 taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt gtaggtaaat
     1501 tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt tatcctcaac
     1561 ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct tttggaagac
     1621 tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa agatacctaa
     1681 caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt gattttatgt
     1741 ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc gttccaatgg
     1801 cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat tggcatatga
     1861 ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac cattaatttc
     1921 ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc agggaagaga
     1981 actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac ctctgtaatc
     2041 atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat aaagcatatc
     2101 gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat atgttttttt
     2161 ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata tgatgttcca
     2221 tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga aaggacgaca
     2281 acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac aaaaaatacg
     2341 agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt tacgatcatt
     2401 attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat ttgaatcgaa
     2461 gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt ttgttgactt
     2521 tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct cactattagt
     2581 agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc attttatttt
     2641 actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg cttctttgta
     2701 cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga acaagaattt
     2761 ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag ttctccttta
     2821 cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt ttttgaaagg
     2881 catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta gtcttttgat
     2941 cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg cattttcttc
     3001 tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag tagaccgaat
     3061 cattggctca ttgattggat gtgttttatt tattggtatc agccaattta ttccttttaa
     3121 ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata gatataaaac
     3181 agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg ttccaggagc
     3241 agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca tgtatattgg
     3301 ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc attagatgag
     3361 atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg ctatcattcc
     3421 atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta atttatactt
     3481 tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt cgattgcttc
     3541 ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg gaaagtaata
     3601 gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc tttcgaccat
     3661 ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat tcactgtgat
     3721 actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg ccagaaatta
     3781 ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata ttttgaatag
     3841 aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga tttatgaaaa
     3901 aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca aagacaacgg
     3961 atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct acagaaggcg
     4021 ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa acgacgatgt
     4081 ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt agtatccctt
     4141 tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg atcgaagaga
     4201 acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc tgttgagata
     4261 tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg aattgttatt
     4321 atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag aaaaaaacgg
     4381 aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac aaagactgac
     4441 atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag aaccgcaagg
     4501 attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt tggaagatgc
     4561 cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca gtttgacaaa
     4621 ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg tttctaagca
     4681 tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta tcatggatac
     4741 tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa gccgtttttt
     4801 cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa gagaagctaa
     4861 gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt tggggatcaa
     4921 acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag tttctaagcg
     4981 agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg caggtgtatc
     5041 gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt cgaatcacaa
     5101 agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga aaagattgtc
     5161 tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac tactcgatca
     5221 tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc cattgactga
     5281 ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt tcagcaacat
     5341 gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg ctttgcatct
     5401 catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga ttatctgtgc
     5461 aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat tcggtaaaaa
     5521 tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt taaaaggaat
     5581 cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc cagttttaca
     5641 tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag ttggagaatc
     5701 tataccctat cattcctcag atagaccgat accagcattt tctctacagc aaaaaaagca
     5761 gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg aaccgaaaaa
     5821 ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag aaggatatat
     5881 aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca tattcagtga
     5941 tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg tagccgttgc
     6001 attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg tttttttagt
     6061 ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa tcgttaggtt
     6121 agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg agaatttcag
     6181 cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa aaataaacag
     6241 tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg cacggacact
     6301 gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag aagagaagtt
     6361 gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac ttctgaaaga
     6421 atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac aagatcatct
     6481 gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt atcaaaaaaa
     6541 taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg aattagaaaa
     6601 aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac gttacctgac
     6661 tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag ctaaaagttt
     6721 aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag gagaacaagt
     6781 aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt ttttgttcca
     6841 gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta cattcttgta
     6901 tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg ttgtaagcgg
     6961 tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca accaagacca
     7021 acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg agaataacta
     7081 atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc gcaacttaaa
     7141 atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat tgtaggctcg
     7201 atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg attttttaat
     7261 gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac gatggggatt
     7321 ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga acgagatgaa
     7381 caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc attctttatg
     7441 tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc tatcaatgag
     7501 acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt aggaacttca
     7561 ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg tatgtgtgtc
     7621 aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc tcgttctttc
     7681 actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa tggattatta
     7741 gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt tgttacgcag
     7801 ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc attgtggatc
     7861 gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt attagctaat
     7921 atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa caattcttat
     7981 gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc attcctcgcg
     8041 aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt attcaatatc
     8101 aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc ggttccgttt
     8161 ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct ggaaatcgtc
     8221 aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc atttgttggt
     8281 agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat cgcattcatt
     8341 atctggttcc catttatcaa attctatgat aataaattat taaatgaaga aagagaaaag
     8401 gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga actgtgacaa
     8461 aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa ataccattat
     8521 tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc tacaccattg
     8581 attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg cggagcgctt
     8641 tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac gatcaaatct
     8701 ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc aaatgctggg
     8761 tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc aaaatcatgt
     8821 cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg gccatctgga
     8881 tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg tgcatagtcc
     8941 acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg tacacgagct
     9001 tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc ggcaatgatc
     9061 gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata agcagtatcg
     9121 cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc tttatctgaa
     9181 cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg aatctggaat
     9241 agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa aacaactgtt
     9301 tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt cggatgttcg
     9361 atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa agtcaagccg
     9421 tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg agtcatacca
     9481 ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt tgtcaaatgg
     9541 gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa tgctgttttt
     9601 ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt tttacctttt
     9661 gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa agccatagca
     9721 agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga attttcgtat
     9781 ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa atattcttct
     9841 ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct tgaatcataa
     9901 cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct tgttgaaaca
     9961 aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa agagaagctc
    10021 attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact ctttttaata
    10081 tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg acacagggga
    10141 caagcggatg agtattttca taaacaatga actgactgta atagatagga gaacgaatag
    10201 tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat tggtaagtaa
    10261 aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg gtatcgttat
    10321 accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga acaaaagact
    10381 tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa caatctctaa
    10441 aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt ttcctaatag
    10501 gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg agcgttggat
    10561 agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag ctatatcgtg
    10621 agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa atataacggg
    10681 tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat aaaaggctgt
    10741 atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt gttcttataa
    10801 aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata gtaatgtttt
    10861 gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag atcaattaac
    10921 accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata tcatttcaag
    10981 attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa atgctaaggc
    11041 tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac tgatctctac
    11101 agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat tgaatgaata
    11161 tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt taggcctgtt
    11221 gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg gtggcggaga
    11281 agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc cttgtatgag
    11341 tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc tgtcgcagat
    11401 tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca tcatgaaagt
    11461 catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat ttatcggtat
    11521 gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt atacatcggc
    11581 gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt tgttagatac
    11641 cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct tcaaattagt
    11701 cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca atgcacagtt
    11761 actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg tagttattgc
    11821 attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg ttacgttgaa
    11881 agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt cattttttat
    11941 gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat tattttatac
    12001 accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac tcttcctagg
    12061 actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg cggcaattaa
    12121 ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca ttcgtttatt
    12181 tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt gtaccttgat
    12241 cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc gcaggacatt
    12301 gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa agatgatttt
    12361 tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc ttgtagtagc
    12421 aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg cagaaagact
    12481 actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag tgaatcagaa
    12541 gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata agtttttagc
    12601 agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag gaacgatcga
    12661 agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg aagatgcggt
    12721 tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt tgcataaacc
    12781 tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg atttattgga
    12841 agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag acacagaggg
    12901 cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac ctaaaaaaca
    12961 tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag atatccgttt
    13021 atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa aactccaaat
    13081 tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc atgaaggaaa
    13141 gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat atttaaaaag
    13201 aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat atcgacccat
    13261 gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc taaaagaata
    13321 agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt tcggatggct
    13381 tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg tttgttcgga
    13441 ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga ctcagagtta
    13501 gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca caacctctga
    13561 taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga
    13621 actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca caacctctcc
    13681 tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg aaagcgctat
    13741 aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag gggttcaatc
    13801 atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta ccgggtatgc
    13861 tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca ttcaaaaatt
    13921 ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg gtgggttggg
    13981 aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa aatacaattt
    14041 tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt gcaatcatta
    14101 tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc ttactacctt
    14161 attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt cctgacaaag
    14221 gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg tataatgaac
    14281 cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt ggttataaca
    14341 gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa gctgctatgg
    14401 ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa cttattccac
    14461 taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat tttggcctgt
    14521 tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt ttggatacgt
    14581 atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct gcaggacttt
    14641 accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc cgacgttttg
    14701 atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag tggagagagt
    14761 ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga ttgcactctt
    14821 tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta ccgatgagtc
    14881 catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat ttggttatac
    14941 gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca ctgtacttgt
    15001 tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt atgcgatttc
    15061 acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta cgatgttgtt
    15121 ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt taaaagatac
    15181 gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg tgatgcgcac
    15241 attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg atggagctag
    15301 cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta ttgcgacaat
    15361 cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac tatacatcca
    15421 agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta atattcaata
    15481 tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct cagttccagg
    15541 agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga tcagctatcc
    15601 atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag aataaagaaa
    15661 caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag aaactagcct
    15721 ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt tctggttcta
    15781 aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc ggagataaac
    15841 cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa gagatcggtg
    15901 taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg aacgtcatca
    15961 tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc aatgcccaaa
    16021 aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa gcttataatg
    16081 atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat gctttcccag
    16141 tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta ctagataaat
    16201 acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc ttgaaggaat
    16261 tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc aaagtccaag
    16321 ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg gatggagatg
    16381 attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta cgtacaatgc
    16441 atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat aaggattatc
    16501 cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat gattatggcg
    16561 atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt actgttccac
    16621 taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact tcaaaaaata
    16681 aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta ttgaatgggc
    16741 ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat gataaaatca
    16801 aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca ggaaacaaca
    16861 agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt gatcaatcga
    16921 ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac agtgtcaaaa
    16981 cagaattaag taatatttct aatgtcatga atcaatattt agatggattg aatacaggga
    17041 ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag gctggctatg
    17101 ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa taataatgaa
    17161 taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga ataaacggtg
    17221 ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga aagccatatt
    17281 cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct tggtaaacgg
    17341 tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga ggaactattt
    17401 ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt tttttgtata
    17461 atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg ttttggaaga
    17521 gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga tatatacatt
    17581 tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg aactatttta
    17641 tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata tatataaaag
    17701 aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg ctttattgag
    17761 ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta ttgattttat
    17821 tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga ttttagatag
    17881 tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg ttttttccag
    17941 cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg tgacttggca
    18001 attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga atggtacgat
    18061 gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat aagtttcgga
    18121 ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg gttatcatga
    18181 tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa caaaatcaaa
    18241 aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg ctgaatgata
    18301 aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa aagatcgaaa
    18361 atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta tacgaacagc
    18421 aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc tacgcaaatt
    18481 atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat tatgaatctt
    18541 ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta gacgcctttt
    18601 atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt attggttttc
    18661 aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga ctctctttat
    18721 acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa atagctaaag
    18781 aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat tcacaattgc
    18841 cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct gatgattatc
    18901 gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa aatcttgccc
    18961 cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt tataaaacat
    19021 tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa acagcagcag
    19081 ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgaag gaactttcaa
    19141 tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc tacaaattag
    19201 aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat ccacactttt
    19261 tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt gaagaattag
    19321 cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa gagaaaacga
    19381 tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat caaatgcgtt
    19441 atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag atcgaagtac
    19501 ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt gattttactc
    19561 gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta aggatcatca
    19621 tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa gaaaaactgt
    19681 ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat gaacgtttac
    19741 gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca ggtggactga
    19801 ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg tagatgatga
    19861 atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag gctttcaaat
    19921 agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc gacagattga
    19981 tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta ttgaagcggc
    20041 tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag aattcgatta
    20101 tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg tgaataagtt
    20161 cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa atgaacaaaa
    20221 aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg aagaattgga
    20281 tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata ggatagtatt
    20341 gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag aacggcaaca
    20401 aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt tgttggaggc
    20461 agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc aggaatggct
    20521 gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat tccaacaagc
    20581 gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg ttttttacgc
    20641 agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac gcgtgctgca
    20701 aaataaacaa ttagaagtcg ctcaaaaaat gatcactgat ttttttgaac agtttcagtt
    20761 agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta tggagattca
    20821 acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa aaatccatca
    20881 ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa agcatcagag
    20941 acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc agcattatca
    21001 agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca tgtatctagg
    21061 acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc atttacgtat
    21121 ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga tagcaagtga
    21181 gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg tcggcagatc
    21241 ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa tgtaatgaaa
    21301 taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac ttgaggcaag
    21361 gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc ggtattaagc
    21421 caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat actcgaaact
    21481 aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta
    21541 gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta atactcaggt
    21601 cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca tctgcggtaa
    21661 taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc ttattacttg
    21721 atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga aaaaggcaaa
    21781 tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga gatcgtgccg
    21841 cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctttgtc catccgtttg
    21901 attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt caattccttt
    21961 ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac atattcaccg
    22021 gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc ggccatactt
    22081 ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg agtcatcaac
    22141 tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa gtaaggatag
    22201 tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc catctcactc
    22261 atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg attgcacagc
    22321 tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag tcctgaaaaa
    22381 tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc ttgtccccaa
    22441 cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg cataaatcct
    22501 gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt cttcgtcatt
    22561 tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata agcatagtct
    22621 aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt caagtaatct
    22681 tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc tttttccatt
    22741 gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc atctgcaatg
    22801 accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag ccattttggc
    22861 aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc gtatttttct
    22921 acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa cccattattt
    22981 gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat tttttgccca
    23041 ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa attatgataa
    23101 aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa ataggactgc
    23161 cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc tagttccagg
    23221 ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt ttgttttttt
    23281 gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc taactcatca
    23341 attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg atcttctgaa
    23401 ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa gagctgatgg
    23461 cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat agataagatt
    23521 ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg acagatcgac
    23581 agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc atggaaaagg
    23641 gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa atcacccatc
    23701 caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc aggatgaaac
    23761 caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc aaaaggaacg
    23821 ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt tgcatgtcta
    23881 gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt tgatgaatga
    23941 aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga ccaaagcatc
    24001 tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg gcgggtcaac
    24061 aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag ggatatgata
    24121 accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt cacaaacaaa
    24181 agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg cgcttttgtc
    24241 atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt aaccaaggta
    24301 acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga tttttgaatg
    24361 tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat cggaaagaac
    24421 ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga attctttcaa
    24481 tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat aaatcaatcc
    24541 gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa gcgtcgtcat
    24601 catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa aatcaccaac
    24661 agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg taggagaagc
    24721 aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa cgcgataaat
    24781 aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc ttactttata
    24841 ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt caccagttaa
    24901 aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgattca tataaacacc
    24961 tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa ataaaaagat
    25021 attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa gatatatcaa
    25081 atagaaagaa ggttttatta gtggcttata aagatatacc aaaatcggtg cagaaattta
    25141 tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag aattttacag
    25201 cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga accacctttt
    25261 tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa gttcgtcctt
    25321 atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg ttagtcaaaa
    25381 agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa gaagctaacg
    25441 gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa agaaaatatg
    25501 aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa aatacaggaa
    25561 aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg gacgtgttca
    25621 aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg acaagaaaag
    25681 aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga atgacatggt
    25741 ctggtttccg tcctagcgat gatacttgcc gttatggata tctggtacca tccaatatgt
    25801 ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc gatgataaag
    25861 aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt cagaatcacg
    25921 gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat ggaatgggaa
    25981 acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct tacttgggat
    26041 attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg agtaaagaaa
    26101 atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat acgccagaaa
    26161 aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac aaatctgaaa
    26221 aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt atgcacgaag
    26281 gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg gcaaatatga
    26341 tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag gaggtatcgt
    26401 aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa tggtacatgg
    26461 cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta gagctatttg
    26521 aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt ttagatgact
    26581 atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat gaaggaaaat
    26641 tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg gaatcgaacg
    26701 tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct gtcatgttag
    26761 gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg aaacaagctg
    26821 gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat aatcaagttt
    26881 tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaagga cctgatcaaa
    26941 cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag attccagtag
    27001 aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa caatatgcct
    27061 ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa aaagatatat
    27121 caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt cattctaatt
    27181 ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc tcagtagaag
    27241 gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca gcatctgctc
    27301 gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat attacagaac
    27361 cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa ttagattatg
    27421 cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt tcagtcgatt
    27481 ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga aagtatcttg
    27541 cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc ccaaaagaca
    27601 gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa gcagaaataa
    27661 ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta tatcaagagt
    27721 tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc actataccag
    27781 ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac cgtttccgtg
    27841 tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg ccagcatttt
    27901 cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag gaaacaatga
    27961 ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt gaaaaaaatg
    28021 gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg catatttttg
    28081 aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt gatcagccaa
    28141 tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca ccggaaatag
    28201 caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag cgtttagaaa
    28261 aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc aaagaaaaac
    28321 gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa atcgactttg
    28381 aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt cctacaaagt
    28441 tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc ccaaaccatg
    28501 tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt gtcaatattc
    28561 atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat gaagtaacag
    28621 aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc gattggggat
    28681 attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac agcattgaac
    28741 tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca cagattcctt
    28801 tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa caatatttaa
    28861 cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt agtgataaag
    28921 tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata actaaagaca
    28981 atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg gttgtcccaa
    29041 taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa tgtatggcgg
    29101 aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag aaattttaga
    29161 aacgccgatt gtaaaattaa gctagacaac taaaaaagtc atttgtgcta cactcaaaat
    29221 agttccgacc aaagaattat aaggagtgag tacaaatgac ctatacccat cttacaacgg
    29281 atgaacttgt aataatagag tcttatttca aaatgaatca atctgttgct aaaactgccc
    29341 attgcttgaa tcgttcaaga caaacgatcc ataaagtata tctgttcttc aagcaaggaa
    29401 aatcagcctt agaatattat caacagtata agaaaaacaa atcaaactgt ggtagacgtc
    29461 cgcttgtttt acccgaggaa caatcagaat atattcaaag aaaggttgtt caaggatgga
    29521 cacccgatgt gattgttggt cgtgcagcgt ttcctattag ttgttctgct cgtaccattt
    29581 atcgtatgtt caaaaagggg ctatttgatt cttctgactt accgatgaaa ggcaagcgta
    29641 aaccgaatgg acatcaagaa agacgtggaa aacaaacttt ccgccgctct attcatgaac
    29701 gtgaaaagga ttatagccaa ttctcaaatg agtttggtca ccttgaaggt gacactatcg
    29761 taggtctgaa acataaaagt gctgtaatta ccttagttga acgattatca aaagttatca
    29821 tcacattgaa accgtgtggt agacaagcga ttgatattga aaaaaaatta aatcaatggt
    29881 ttgaatctgt accgaaaaac ctattcaaat ccatcacttt tgattgtgga aaggaatttt
    29941 caaattggaa acagatcagt aatgtcaatg atattgccat ttatttcgct gatccaggaa
    30001 cgccgtctca aagaggccta aacgagaatt ctaacggatt gttacgtaga gatggtttat
    30061 tgaaatctat ggatttcaat tcagtagatg aattttttat tcaatctgtc gcatctaaac
    30121 gaaataatat tcctagaaaa tcactggatt atcgaacacc tttggaagta tttttgagtt
    30181 acgtaagtat tgatgatctg tctaacttaa tttgacaatt aagaatacaa aaaagatgtt
    30241 ggtgaagttc accaacatca aaaagtatag agccaaaata ttccaacatc atcttggact
    30301 ttctacatgt tactaatgag cgtgtaaaag aacgagatgg tgtttttatt tctatcaatg
    30361 aagctctttt tctttgcaat cctcacttca atcctctata atgtcgcata tagtttagta
    30421 aatgagtaaa ctactaattt acttatttac taaaaataaa aggatgagga gttaaaataa
    30481 tggaaatacc aacaaattta aaacataaac cagtaatcgt tgtagaagac tatgataaag
    30541 ttgatggaag aaatgcatta aatatggatg caaaagggct ttcactgggc cttgctcagt
    30601 ggaatgatcg tggaaaagtc gatatctcag gcaaggtatg gcgacatact ggagaaaaat
    30661 ggtcacgaca gtcggaagaa ttaccaatca cacgtatttt agatttagcg atcttagctg
    30721 cgcaaggaag tttatacttc caagatgctt atcgttatga aaaattttat gatccagaaa
    30781 atccagtcgt agacatcatt ggattacaag ggaaccgaat gacggtagaa gtagatacca
    30841 aaaatccaaa gatcgatgaa gacatcattt tgtattacga tacactacaa aaagacggag
    30901 aactgattgg ggaacgattc cgtattttaa aacgtctttt agatgagcta ggatattaat
    30961 agacagagat gatcaaaagg ggaagagaag catggagaat ttctcactaa cagaaaaccg
    31021 aaccttttca cggctagaaa agcaaatgat ctggaagaaa ccgaaaagcc atcggacaag
    31081 tgcagaagaa tggcggatcg cctccgaaat caaagggaat tggcaagatc ctgaaatgaa
    31141 aatcagtaac gtacttttgg aaggagatgc cgggtcgggg aagacacaat tagccaaagc
    31201 actatctgct gatctgcaac ttccctatac aaaagtgacc tgttttgcag atatggacaa
    31261 atcagatgtt ttcggagcct tattacctgt agtagattct aataaggaac aagaccaaga
    31321 attactagaa gctatttacc aaacagatac acttgaagct gtactgacac tgatccagaa
    31381 ccattacggc attgatcctt tttctgctaa agaaaaatta gcagagctag ttcaaaggat
    31441 cgaagctgat tatccagatg caatccatta cagctattac ccttctgaaa tcgttcgagc
    31501 gattgaaaaa ggctatttat tagagattca agagccgact gtcatccgag atgcctctgt
    31561 tcttgtggca cttaactcag cgctcgaacc aaatgggatg ttgaatttac cgacaggaat
    31621 cattcgtcgc catccagatt gcgtcattgt gatcactaca aatcgaaatt accaaggaaa
    31681 tcgcccatta aatgaatctt taagagatcg gatgcaacat gcggaaaaaa tggatcttcc
    31741 tgccttagaa gtcatggcag aacgtgcgat ggcgaaaact atgatccaaa aacaagaact
    31801 tctcttaaag atggcagaga ttatccgttt gttggatgaa actgcaaaag ctaacgccat
    31861 caaaggtgta gcaggaatgc gttcttattt ttattgggca aatacctata agcaaggaca
    31921 agatttactc cagtcgattt atccaaaagt tttgtataaa ttatccactg attcagatga
    31981 attggcaatt cttgaacaag ctttgtctga cagtggattg ctactggaac ttcaggacat
    32041 attgagaaaa gaaaaatggg gtcatcctca atctgagcat acaaaagaaa gaaccattag
    32101 cgccgaagaa gcaaatgaac gaaacatcac tgaaaatgct gaagaaattc tgcgcttaga
    32161 tgaaaagaaa tcgttgtctg ctgaagaacc aaatgaggga gaagagaaac aagaagaaac
    32221 aatagaaaaa aacgagattc ctcctcaaat gcaaaaagaa cgcagtagcg atatcgaagg
    32281 aaaaaatcaa aaagaacaac aaacaggaga tgattctcaa gaaatgtctg cttctgacat
    32341 ggaagcacat gataaggaaa tcaagaagca actgaataaa gaagcgcgac aaatcatgaa
    32401 ggaaacgatt catgaaaaag aaggtctcat cgttcaccga ccagctttcc aaggaaaaac
    32461 gccggaagca cgtgagctac acatgcaagt aatgccaata gtagaatcac ttagccggca
    32521 aattttagag ttactggata atgaacaaac agagacgtac caaaaaggaa aatacgaagg
    32581 tcagcggttc aatgcaagca aagtagcata tggtgatttg agaaattttg acaaaaagaa
    32641 cccgccacat gagcaacctt ctttagcggt agctgtacgg atcgatgaat ctgggtcaat
    32701 gatacgggat gatcggatcg aagcggcaaa aaaagcagcg attgccattg ccgaattttc
    32761 taagaaagtc gatatcccat tgttgattta cggagataca gcagaccgtt cgtcaagaga
    32821 aaagacttct ttgttttctt ataaagaatt tgaagacgga tttcaatggt taaatgaaaa
    32881 attagtcact atgaaaccaa ggcaaaacaa tcgtgatggt gctgcacttc gtttagcagc
    32941 agaaaaatta aacagacaat ccgcaacaac caaactgttg cttaatatta gtgacggtca
    33001 gccaaaagcg ttgccggatt atacaggcaa aaaagcaaaa gaagatatcc agtctgtttt
    33061 gaaagaatat gaacgtcaag gaatcctatt tgtctccgca gctattggcc aagacaaaga
    33121 agaaatcaag tcgatctacg gtgaaagtcg ttttgtagat attactgact tgaatgaatt
    33181 tcccaagcaa atgattcaac tgattgcccg ctatctatga taaaataaag ctcaacgaac
    33241 gcacttagca gattcgttga gcttttcgtt tctagttacc ggtaacaagg atacttctaa
    33301 aatagaatcg gttataatca agagggaccg ttaaaggagt gaaacgatat gatacaagaa
    33361 caaaaatggt ggaaagaagc agtaggctat caaatctatc ctgctagttt caaagatagc
    33421 aatggcgatg gtcaaggaga catcaatggg atccgtgaaa aacttaatta tttgaaagat
    33481 cttggtatcg gctttatctg gatcaatccg atctatcaat ctccatttgt cgataatggt
    33541 tatgacgtaa gtgactatca agatatagaa gagagattcg gaacgatgga agattttgat
    33601 ttattgctaa aagaagcaca tgaattaggg atcaaagtga ttatggatct tgtcatcaac
    33661 catagttcgg atcagcatca atggtttgaa gaatcgaaaa aatctaaaga aagcccgtat
    33721 cgcgattatt acatttgggt cgacggggta gatggcaaag aaccgaataa ttggacatcg
    33781 attttcggcg gttctgcttg ggagtattct catgaaactg gacagtatta tctgcatgtt
    33841 tttgcaaaag aacaaccaga cttaaattgg gaaagcgaga aattgaaaga agaactattc
    33901 aatatgatcc gatggtggtt ggataaaggg atagatggat ttcgtttaga tgcgatttct
    33961 catgtaaaaa aagatgaata ttcagtaaaa gccacagaga accctttctc accctttcaa
    34021 aatgtctcag gaatcgaaga acatttgacc gatttgaaac atgtttttga agagtacgat
    34081 atcatgactg tcggagaagc aagtggtgtc actgcagaag aaggaccaca atgggtaggg
    34141 aaagacggtt atttcgatat gatattcgaa tttgatcata tccatatctg gcagcaagaa
    34201 aaagaagggc aattagatgt ccttaaattg aaacatgcat taagcgcttg gcaaacttca
    34261 ttggatggta tcggatggaa cgctttatat atggaaaacc atgatgttcc tcgtgccgtt
    34321 tcagtgtttg gagatactcg tcccgatttt tgggctatgt ctgcaaaagc aatcgctatg
    34381 atgtatttct ttttgcaagg tacgccattt atttatcaag gacaggaaat cggaatgacg
    34441 aatatgccct ttgaatcgat cgatcaagtt gatgcagtag attcgaaaag actttataaa
    34501 aggctgcttg cggaaggtaa gacaagagaa gaagcattgg atatcattcg tgaaacaacg
    34561 agagacaaca gcaggacacc catgcaatgg acttcggaac aatatgctgg tttttctaca
    34621 catgaacctt ggctgatcac taatccaaat accaaaacga tcaatgtaga acaacaagaa
    34681 tatgaaccag agtcggtcct gcaattctat aagaacatga tccgcattag acaaaccaat
    34741 aaaggtctga tatacggttc ttataaagaa tatcttcatg aacatccaca actttatgtc
    34801 tatgaaagat atttagaaga tgaacaatat cttattatgg taaacttaac agaatcactt
    34861 gctgactatg aacttccaaa agaagcagat cagtcctgga cattactctt gtcaaattcc
    34921 tctagcggag aatttgaagc taaaggcata ctagcacctt atgaagctcg gttatataaa
    34981 acaaacaagt aaaacttcct cgttaaagtt cattagttgg actttgcgag gaagtttttc
    35041 agattgctct gtcaattact catttcaata attcgctcta ggaccaccga tatatttagg
    35101 acgagagcga agggctgtca cgtgagcttg tccaagggtt ttcaaacgag gtctgacagg
    35161 tacttggaca tgcttgatat gcataccgat tgatgtgtca ccgatatcga taccggcttg
    35221 tccaactacg tgttccactt cgacaggatc attgaactgg tcaaaagcag caacagaaca
    35281 agcacctcca gcatgtagtg caggaacaac agagacaatt tcgaaatttt ttgcttctgc
    35341 cactgttcgt tctactacta atgctcggtt caaatgttca caaccttgga cagctagcgc
    35401 aatttcttta ggatccaaca actcttttaa cgtacggatg atccattgac caacttccgc
    35461 actggaattt tttccaatat gtcctccaac gacttcgctt gtgctgcatc ctaaaacaaa
    35521 aagatcacct tttttcaagt gtgcctcagc caatatgtca ttaactattt cagtcaattg
    35581 gtcttttaat tctttttctg acaacttcat tttctaaaac cttctttttc aattaatatt
    35641 gcttccgtgc ttgaatacgt tgaagacttg cagttatagg aaaggcaagt cccattccaa
    35701 tcagattttg gaagatgttt cctggtatag aagccactcc agccggccaa tcatataaaa
    35761 tccaaccggc gataccatag ccgatgatca taactaagct tcctaataaa agccctaatc
    35821 cccacatacg agtagaagat ttgctgaaag aaccagcaac atatccttgt aacccgtgga
    35881 tcactaatga aaaaactgcc cactgcggat atcctgaaat caaatcgatc aatagaccac
    35941 tggaagcacc aacaactaat ccacccgttg gaccataaag aaaagcagcg gtatagatcc
    36001 cggcctcgca taatgtcaca aatccattcg tagccggtac agggatcaaa attgttaatg
    36061 aaagggcaac tgtcaacgca atcagcatag catagatcgt taattgctta gttttcattt
    36121 ctcattcctc ctttttgaat accgccagac cagacacttc cggtatgatc gttcatcagc
    36181 acgccattag aaatacaatg ggcaacaaat tttttacttt ctgctactgc tttggtaaaa
    36241 tctgccccta acacaagatt cgctgttatt gcagaagaaa aggcacaacc ggccccgttt
    36301 actgttgtag tgtcgatttt tttatgagaa aatacctcat aatgatcaat tgaatagaga
    36361 agatccacag cttcatctcc agccatcctt gtgccgcctt tgatcacaat gttttttgtt
    36421 cctgaagcag tgagttcttt agcgagatgg atcatttctg ttaacgaaga gggaagagct
    36481 tgaccagaaa gtaaagcagc ttctgccaaa ttaggcgtaa tcatcgtaac aagtggaaaa
    36541 agttcttcga tgagctgcga aatatacaat tgattgatca cagcttcagt ttctttgaat
    36601 gccaaaacag ggtctaaaat gattgggata ttttgttttg tttttatgaa gtctcgaaca
    36661 accatgattg cttcacttga attcaataac ccaattttga taccatctaa tgaagtcatt
    36721 ttatcaatcg aaactaactg tcgtttaagc aattccaaag atatagattc cagcataaat
    36781 cgctcattaa tatctagtgc ccctatagaa gtcaaggcgc ttagtccgaa tagaccaaaa
    36841 ttctcaaaag tttttaaatc tgtttggatt cctccaccag caaatggatc agaaccgcca
    36901 attgtcaaga ttcttttcat aacattttcc tccatgaata agagtataac gaaattttca
    36961 aaaaagaaaa acagccaatt ggcatatttt tatctcatcc aatttaaaaa aacaaaaaga
    37021 aaagaaagac caaaatggat ctttctttcc ttaaatagtt aggacttttg tgattttaat
    37081 tctttctcga tttgaagccg ttgcactttc attgtagctg ttcgcgggat tgcatcaaaa
    37141 tcacggatga tagggacgtt caagcgagga agttcatgga cttgctccca ccatcgattc
    37201 caatccattt ctttatcagg agctaaagca ataatcggtt gtggagaatg atttttatcc
    37261 cttacaatca ctacttctgc caaaaaatct aacgaatcta ataaaaagtc ttcaatagct
    37321 aatgtactat tgatcgtttc gatcaaatcc acttgccgat ctttcaaaaa taggtggcca
    37381 tgcttatcca taaaaccgta atctccgcta tcccaccaat cgccataaac attttcctgg
    37441 aaccgtgcat cttccttgta ataagtcaaa gcccgtcctt tagacagtaa ttgtatatga
    37501 ccatctgtgt tttcaggtag gacattccca actgaatctg taatccgagc gcttgtcatg
    37561 tcttccagtc caacgcccat atctcttgca tcacttgttt tcaaactctc tagtgtatga
    37621 gccttaagaa tcattggtcc gcattcactt tggccatata cttgaagaaa taccgcatcc
    37681 tgtgccatcg acgttcgcaa aaatgtcttc attgtctggt tgtttatagc atcaaaagtg
    37741 gagtgatagt atcggatacc cgaaaaagct tctggatgct ccttagcagt aaaacgccac
    37801 tgaacaaagt tattcggatg ggtctctagt gcaattggcc ggtgagcttc caacatatga
    37861 acaacttttg aactttgtgc gtttgctaaa ggcatcatag gaaatcccat ggacatcaaa
    37921 gaagagatac caatattgaa tctggaatgc actggagaga tgtgaaaagc gattaatttc
    37981 ttttccgaaa tttttgtgaa aactgttttt tgccatttcg tccgccatcc catagaatga
    38041 gcagaatgac aaataagttt agggatacca gttgtaccag aagtatgggt catataagaa
    38101 atttcatttt tgtctaaaaa tgattgagaa acagacaaag caggttgttc cattaaataa
    38161 agaataggaa tctgttttga ttttgttcca tttgaaattt ctctcactcg cttttttgtc
    38221 tcatcatcaa ataaaatgaa agggtcttct aaacgatcga taaatacttc cattgttgtt
    38281 gttggtagat gataagaagt catgacaggc actgcaccaa ggtaagacac agaaacagct
    38341 agaaggtatg tgtcaaaagc ggaactctta taaataataa ctttgtctcc ggatttcact
    38401 ccaagatgag ccaattgata agcccgtttc aacacacttt cgcagcattt tttgtaagtt
    38461 gtttcaagtc ctaattctgg gaaagctgaa agtggctcat caaagataat cggggtctcg
    38521 gaaaagttag ctgcagcttc ttgaaaattc gtaaataagt tcaaagggga atacgttatg
    38581 gtctccatca ttcacactcc attatctttt gttaagtaat atacaaattc aagataatcg
    38641 taacataatc ctttatataa taacatagtt tattgtgaaa aaaagatcat acatttgttt
    38701 tatatggaag aaacccgaac gaatgccgct aattcgtgat aagaaaaatc aaccatcttt
    38761 tcccagatat cttgaaaaga atgggatgag aggtatcgat taccacataa ataatttacc
    38821 gcgtcagcat catctaaata tagttctgca taataacgat atgtttttct gcatagttct
    38881 ggcgatacgt ttttatatac tgtaattttg ctcaaaattt ggcgaatagt ccgaggagaa
    38941 atttgctgat catgtgagga acgaaaaaca aaagaatttt cgtccgtatt ttgtgctgtt
    39001 aacttaaaat agttctccag aaatggcatc gcttcttttg gtattggtac tttattcagt
    39061 ctgttttctt ttctattcag attaagaatc tgcttggtca aagatacatc tttacgttta
    39121 agttctacta cttcatggat ctttaatccg caatacagaa gaatggcagt aattgcaagg
    39181 ttcctcggtc tattcttttc ttcaaagcta gcttctctgc ttgtttgaaa gccttcaatc
    39241 gggataaaca aatgctcaaa atctttgaaa agttctgcat cgatatatcg cggagctgct
    39301 ttggttgttt ccggtaaact tgtacccaaa agagaaatct cttcttcaat cagtcctttt
    39361 tgatacaaaa atcgtaaaaa atgatttaat gaatattgct tccttgaaat cccgtaatag
    39421 ctattttcaa acaattgctc cgtttcttgg ttcgctgtag tttggaacga acgagtatgt
    39481 cgatagattt tagctaagaa attctgataa tcttgttttt ctaaatggat aatcagctga
    39541 tcatccactt cttcagaaaa tacttttctc aaataatcag taaacagatg aaaatctttt
    39601 gcgtattcat agcgactgcc taatgaaagg gaagaacgat tttcttctaa aaactgttga
    39661 aagacggaag ggagcaagtg tgtacgttgc tttaactttt cttgcagttg ttttctagtt
    39721 ttgctgtcca ttacaaattc cctccaatag tgttcatttt ctatatttta ccgcatttca
    39781 tcaaatgaag atagacagtt tgtcttgatc ttaggaagca aatttttaac taagtactag
    39841 ctaaaagata tgaagtaaga agatacattt ttagttattt tattagtgca gtcattcttt
    39901 tgatctttta attgatttat cctcttttta gctatgtggt tacttcatga gcaatgaaat
    39961 ggatttggtt cgttctaatg gcaggaggct ctttctttta tttccgaaaa attagaaaac
    40021 attttttcag aatcgattgg ctctccattt attcaataat ggtttgtcta acgatattgt
    40081 tgggataatt cttaaagaag tattagaaat agttggtact tcattacttt acatgaagat
    40141 ttatt
//
LOCUS       DAZKOB010000015        37136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000015 DAZKOB010000000
VERSION     DAZKOB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.015"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..384)
                     /locus_tag="ACG8M6_000800"
     CDS             complement(<1..384)
                     /locus_tag="ACG8M6_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456489.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="ACG8M6_000801"
     CDS             complement(569..967)
                     /locus_tag="ACG8M6_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0456490.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="ACG8M6_000802"
     CDS             complement(964..1737)
                     /locus_tag="ACG8M6_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0456491.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="ACG8M6_000803"
     CDS             complement(1734..2417)
                     /locus_tag="ACG8M6_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0456492.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="ACG8M6_000804"
     CDS             complement(2584..3471)
                     /locus_tag="ACG8M6_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456493.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="ACG8M6_000805"
     CDS             complement(3468..4337)
                     /locus_tag="ACG8M6_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456494.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="ACG8M6_000806"
     CDS             complement(4474..5760)
                     /locus_tag="ACG8M6_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0456495.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="ACG8M6_000807"
     CDS             6033..6611
                     /locus_tag="ACG8M6_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0456496.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="ACG8M6_000808"
     CDS             6611..7540
                     /locus_tag="ACG8M6_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0456497.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="ACG8M6_000809"
     CDS             7537..9273
                     /locus_tag="ACG8M6_000809"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0456498.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG8M6_000810"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG8M6_000810"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0456499.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="ACG8M6_000811"
     CDS             complement(10169..10678)
                     /locus_tag="ACG8M6_000811"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0456500.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG8M6_000812"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG8M6_000812"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0456501.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="ACG8M6_000813"
     CDS             complement(11979..13070)
                     /locus_tag="ACG8M6_000813"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0456502.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG8M6_000814"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG8M6_000814"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0456503.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG8M6_000815"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG8M6_000815"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0456504.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG8M6_000816"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG8M6_000816"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0456505.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17033..18007)
                     /locus_tag="ACG8M6_000817"
     CDS             complement(17033..18007)
                     /locus_tag="ACG8M6_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0456506.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17988..19871)
                     /locus_tag="ACG8M6_000818"
     CDS             complement(17988..19871)
                     /locus_tag="ACG8M6_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0456507.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19889..22114)
                     /locus_tag="ACG8M6_000819"
     CDS             complement(19889..22114)
                     /locus_tag="ACG8M6_000819"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0456508.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22229..23263
                     /locus_tag="ACG8M6_000820"
     CDS             22229..23263
                     /locus_tag="ACG8M6_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0456509.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23330..24343)
                     /locus_tag="ACG8M6_000821"
     CDS             complement(23330..24343)
                     /locus_tag="ACG8M6_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0456510.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24421..24609)
                     /locus_tag="ACG8M6_000822"
     CDS             complement(24421..24609)
                     /locus_tag="ACG8M6_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0456511.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(25049..25177)
                     /locus_tag="ACG8M6_000823"
     CDS             complement(25049..25177)
                     /locus_tag="ACG8M6_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0456512.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="ACG8M6_000824"
     CDS             complement(25180..26553)
                     /gene="rlmD"
                     /locus_tag="ACG8M6_000824"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0456513.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26664..27719)
                     /locus_tag="ACG8M6_000825"
     CDS             complement(26664..27719)
                     /locus_tag="ACG8M6_000825"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0456514.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="ACG8M6_000826"
     CDS             complement(27729..29159)
                     /gene="gatB"
                     /locus_tag="ACG8M6_000826"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0456515.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="ACG8M6_000827"
     CDS             complement(29156..30628)
                     /gene="gatA"
                     /locus_tag="ACG8M6_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0456516.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="ACG8M6_000828"
     CDS             complement(30628..30933)
                     /gene="gatC"
                     /locus_tag="ACG8M6_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0456517.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="ACG8M6_000829"
     CDS             complement(31039..33075)
                     /gene="ligA"
                     /locus_tag="ACG8M6_000829"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0456518.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="ACG8M6_000830"
     CDS             complement(33092..35326)
                     /gene="pcrA"
                     /locus_tag="ACG8M6_000830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0456519.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35481..37040
                     /locus_tag="ACG8M6_000831"
     CDS             35481..37040
                     /locus_tag="ACG8M6_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0456520.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT        10262 a         8064 c         6697 g        12113 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa ctgatgtaag
    16801 aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg aaactttttt
    16861 taattttctg atagttaact ttgtttttca gaaaaataac aattggtttt attgaagaaa
    16921 atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga catatagaat
    16981 aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat tgctactgaa
    17041 taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac tcaacatccg
    17101 ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta agaaccgttc
    17161 gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct gctttgaatg
    17221 gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa aaatcttctt
    17281 gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat ttcgtttcca
    17341 tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct gccactttat
    17401 aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat aggccttcat
    17461 actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg ggtaattccg
    17521 gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct ccagcgattt
    17581 ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta aaaatgattt
    17641 caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca atcggcaaat
    17701 cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac aatccggctc
    17761 ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg gtcgttgctc
    17821 gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta ttgagcgatt
    17881 ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg acacctaatg
    17941 tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta tttactgtat
    18001 ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc tgcaaaaacg
    18061 atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc gccatagcgc
    18121 atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt aaaatcccat
    18181 atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga atccattcca
    18241 cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt ttccgcttta
    18301 ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt tacatctgaa
    18361 atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag tcgatcgggg
    18421 tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata agattcattt
    18481 ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt cgtttttgct
    18541 cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta agaaggctca
    18601 aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc catcacatgg
    18661 attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg agatgtccgt
    18721 acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc ttctatagtg
    18781 atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat aatcatccgt
    18841 tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc gaaggaatag
    18901 tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag cttatataaa
    18961 ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg aatcgtcatt
    19021 tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc tagtatcgct
    19081 acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc aagtttgcca
    19141 ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg gttttgtcct
    19201 cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc ctgatcttcc
    19261 aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc ttcttccaaa
    19321 taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc gataaaatga
    19381 ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga gatttgtttt
    19441 ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg attgtcttta
    19501 gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg gggacggcga
    19561 aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc ataatttgct
    19621 aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa atctttttta
    19681 taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca tgatccattt
    19741 aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata acaaacatga
    19801 tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa ccattttata
    19861 tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc agttctgttt
    19921 ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg gcccccgaaa
    19981 atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc atcagatata
    20041 cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca atagcttcat
    20101 cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga ctcgccaaac
    20161 gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata atttggtctt
    20221 ttacttgctt ttcttcttca ggagacagct ttgtcaaatc cagttcataa ccaaaaacag
    20281 cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc tgatgatttg
    20341 gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt gttccatatt
    20401 ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc ggcatataat
    20461 aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat aagatttccg
    20521 gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa cgatgcgcta
    20581 cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc gaacggttca
    20641 tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt tgtttctcaa
    20701 tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg gaagcagaag
    20761 gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt tcagaatcaa
    20821 ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta tgagcataat
    20881 ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag tctcctaatg
    20941 aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac atttcgatgc
    21001 ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag tcaaagtaag
    21061 tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga cctcttgcga
    21121 ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc tgacttgagt
    21181 aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc cacgaaaaac
    21241 gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt tcaagttcga
    21301 aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta ttttctgtcg
    21361 tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt cctcgacggc
    21421 tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt tcattgacat
    21481 gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag tctagctgga
    21541 aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc tttacagatc
    21601 gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa atcgctgttt
    21661 ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc acataagttt
    21721 gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca tatctcagat
    21781 ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga aagtccatgc
    21841 tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg tcaggattta
    21901 agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg tctttgtact
    21961 gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct atctttatca
    22021 gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca aataagatga
    22081 aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca aaacggaaac
    22141 cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt caataaaaat
    22201 tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg cacgaaaggc
    22261 aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga tccatgtggc
    22321 tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg acatcagtac
    22381 tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga tcggcaaagc
    22441 gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag aagaagctcg
    22501 acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta aacaatggga
    22561 agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag aatcgattac
    22621 cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca tccagcaaga
    22681 ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg aattgttctt
    22741 agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata tggatgaatt
    22801 cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc attttatgat
    22861 cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag aaaaacaagg
    22921 tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg ccattcttgt
    22981 cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa atatccaaat
    23041 tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa acctctcccc
    23101 tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg ttcgaatggc
    23161 attggagcga attgacggta tacgggacga accaatcatt ttgacttatc ctgcacgatt
    23221 gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag gttgtgaaaa
    23281 gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct tacttatact
    23341 tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc gtgtaatcaa
    23401 attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat tgcacgggta
    23461 cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat gagtatgcat
    23521 ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc acactagctt
    23581 ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac gcaacggttt
    23641 tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc ttcaaatcaa
    23701 caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtagc tgtttgattt
    23761 tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat gcgatttgtg
    23821 aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg tcgtcttttt
    23881 cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta gggtattctg
    23941 aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct gaaactaatt
    24001 ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca ccatgtaccc
    24061 aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca taagtattct
    24121 ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt tctgcttgat
    24181 cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg atcaagaatg
    24241 ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc ccaagaatga
    24301 ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc cctcagtagt
    24361 tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct cctagatgat
    24421 ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt ttttccattg
    24481 ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag gacttaagca
    24541 caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca cttgctggtg
    24601 ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat ttatcttcag
    24661 agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct aaataatagg
    24721 cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc cctgatgccg
    24781 ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa tcatttgaga
    24841 atatgaaaat aaaaatcttt ttgatcaatc agttaatcaa attgaaataa cgaaaaatct
    24901 gagacagaat gacgttcatt ctgacccaga tttgattttg cacaactcaa cagtccttat
    24961 tggcaaatcg ccaaaataat gtagactaac taaaaagact aagaaacaat aacaaaagtt
    25021 aacacttctt agtctttctt tagcacgtct atttattaaa gattttaggg ccttttctca
    25081 tgtttttacg atcattctta ggtgtttcca tttgtttgga tcggcctcca ccttgtttct
    25141 ttttgatcaa ttctttcatt ttttcttttt ggtccatttt tattctcctt taacgaatga
    25201 agcgactaat tcgatatgat gggtttgcgg gaaaagatct acaggctgaa ctttcttcac
    25261 ggcataacct gcttctgcaa ataacttagc atctcttgca aaagttgccg gattacatga
    25321 gatatagacg attcgttctg gtgacatttc gattgctgat tcaataaaat gatgatctaa
    25381 tccttttcgt ggagggtcaa ctacgatcac agatggtttg atcccttttt cttgccattt
    25441 tttcataact ttttctgctt ttccgacttc ataatgaaca ttttcgattt tattatttaa
    25501 agcattgaac tgtgcatctt caattgcttc tggaataact tccatcccat acaccttatc
    25561 cactctgtct gctaaagaca agccaatcgt acctattcct gaataagcgt cgatcacaat
    25621 gtctttttta tctaactcgg caaactcgat ggctgtttgg tacagatact ctgcttgttg
    25681 agtatttact tgataaaatg actttgctga gattcgatag gtttttccta atagctgatc
    25741 ttcgatatag tctctgccat ataaaacttt ttctttgtct cctaaaatca catttgtttt
    25801 ttcttcattg atattttgga tgaccgaaac cacttctggc aattcttcat gaatagcttc
    25861 agcaatttcc ttggatttaa agaatttttc tttacgagta actaaaacga ccatcatttc
    25921 atgggaataa tgaccacgac ggataatgat gtgacgaagc tgtccttcat ttttttcttc
    25981 attatatcct cgtacttgga accgttgcaa gatatctctg actactttga ttgcttgatc
    26041 gatgacagga tcttggatat aaaaatcttc gatagggact agatcatggc tattcttttt
    26101 gtaaaaaccg gtggctaact gtccatcgat acggcgaaca gggatctgtg ctttattacg
    26161 gtagccgaat ggttctgcca ttccgaccgt tggaagtaca ggaacttctg gcatcttagc
    26221 aatcttcgcc attacatgct ctacttgttt ttgtttgaag acaagctgtt gttcatactt
    26281 caaatgggct aaaggtgcga tacctatgcg aagtaatgca ttgttttcta caggctgacg
    26341 atcaggtgat tgtgtaataa atgtttcgac ttttccatat ccaaacttat tgcctgcttt
    26401 tactactagt atttctactt cttctccagg gagtgcattt tcgataaata atggataacc
    26461 atcgattttt gctaccccca ttccttcata actcaagtcg acgatttcga ctgtatatcg
    26521 ttcgtttttc tttactattt gctgttctgt catattttgc tcctttaagc ctatttttca
    26581 atcctactga actctactat aaatcagcta attacacaac taaaaccggc actgacctta
    26641 ctacgttcag caccggcttt tgattaattc ttttgattag gtgtttcgtc tgctattttt
    26701 ccgtcaccgt cgatatcttc atccgttaaa cgttcgactt cttttacgaa ttctttgctg
    26761 acaacctcta attcttcttc atctgtacct gtgattgctt catcaggaat ctgatcggta
    26821 ttggcaaaga actcaatgtg ttgatgaaga ttttggaaat gcatcggtgc ttccccgccg
    26881 tattcaccat ctaagttgat cattactggt cgattgatat catcttctgt ttcaacgaac
    26941 aaactgcttg ttttggtata gattattctt ggatcatcga catgtcgacc gccattcagc
    27001 atgagtgctg caatatgcaa gatctcaaag atatttgctg tcttcacaat gattaaagaa
    27061 aacttcccat catccaattt ggcatctggc gcgatctgtt cgaatcctcc aacagagttc
    27121 gtcagcccta ggaaaaacat cgaagcattt ccgatatatt ccccttcatc atacttcaag
    27181 cgcattttga ttggctttac tcgagggagc atctctgctc ctttcgcaag ataagcaaga
    27241 taaccaaaaa tactctttag ttctgatgga acttcataag tcaattctgt caaatagcca
    27301 cctgcagcga tattgatgaa gtaactgtta tcagaaaggc cgatgtccat ttttacggtt
    27361 tggtgttttt ttatcacttc tgctgctgct ttaatattat ctctaggaat ttttaatgct
    27421 cgagcatagt cgttcgttgt accagctggg atgatggcca ttttaggacg gtgttcaagc
    27481 ggagcgattc cattaaccac ttcgttgatt gtgccatcgc caccagctgc cactactaga
    27541 tcaaaaccta gttctgcgac tctacatgct tcattcttgg ctgaatctgg ttctggtgta
    27601 gttgcatagg cgctggcttc aaatcctgct tcttctaatg cctgtaggat atccgccaaa
    27661 ttttttttca ataattcttt tcccgaggtg gggttataaa ttacgcgtgc ttttttcatt
    27721 ttcagcctct atcgtttcgc taattcatct ttaagtagct tattcacgac accaggattt
    27781 gcttgtcctt tagttgcttt catgatttga ccgactagga agccaaccgc acgatctttt
    27841 ccatttttga aatcatcgac agattgttgg ttgttatcca aaatttcatt aatgattggc
    27901 agtagtttgt tcggatcaga taattgcacc caaccatttt tttcaactac ttcttgggct
    27961 tctccgccat ttgtgatcaa ttcacggaag acttttttgg cgatttttga gctgatcgtt
    28021 ccatcggcga tcaaacgaat cattcctgct aagttctcag gtgtcaattt tgtctcagct
    28081 aattccagtt tctcactgtt caagtaagca gaaacttcac ccatcaacca gttagatgct
    28141 tgcttggcat ctgcaccatt ttccaaaaca gcttcaaaaa aatcagacat ttcttttgta
    28201 agtgtcaata ccattgcgtc atactctgga agtccaaact cacggatata gcgttcgcga
    28261 cgagaagctg gcatctcagg caaactagct tttacttttt gaatccattc gtcatcgatc
    28321 acgaatttcg ggatatcagg ctctgggaag taacgataat cactagaccc ttctttgaca
    28381 cgcatcaaaa ctgttttatt cgttgtttcg tcaaaacggc gtgtttcttg ttggatttct
    28441 cctcctgaaa gaagaacttt tgcttgacgt ttttcttcaa aagctaaacc ttttttcacg
    28501 aaagataaag agttcaagtt tttcaattct gctttcgtac cgaactcttc ttgtccataa
    28561 gggcgaagag aaatattggc atcacagcgc atagagcctt cttccatttt cacatcgctg
    28621 acttctgtaa attggataat tgatcgcaat gcttctagat aagcgtatgc ttcttctggt
    28681 gaacgcatat cagcttcaga aacgatttcg atcaaaggcg tgccttgtcg gttcaaatca
    28741 acgtaagaat aaccgccaat accatgcatg ttttttccag catcttcttc taaatggaca
    28801 cgttcgatcc ggattttttt ctttttacct tctacttcga tttcgatcca tccgtcatga
    28861 ccgattggtt gatcaaattg ggaaatttga tatgcttttg gattatctgg gtaaaagtaa
    28921 tttttccggt caaaatgagt atctttagag atttcacagt ttaatgccaa agctgctttc
    28981 atcccgaatt ccagcgcagc tttattcatt actggcagca cgcctggata accccagtcg
    29041 atcacgtttg tattgctgtt tggttctgca ccaaaatgag ctggcgctgg agagaaaatt
    29101 tttgagttgg tttttaactc gacgtggact tctagtccaa tgactgtttc aaaattcatt
    29161 cgtctttccc tcctaaaata gctggttgtt gtttatggaa ttctgttgct tgttcaaacg
    29221 catatgccgc ttgatacatc gttttttcat caaaataatt accgatgatc tgcaatccaa
    29281 ctggtaatcc ttctgataaa cctgccggta cagacatacc tggaagtcca gccaagttga
    29341 cagggatcgt taagatgtca ctcatgtaca ttgtgattgg atcgttaatg ttctcaccga
    29401 ttccgaatgc aactgttggt gaagatggcc cgatgatcaa gtcataatct gcaaagactt
    29461 tttcaaaatc tcgtttgatc aatgtacgga cttgtcctgc ttttttgaag taagcgtcat
    29521 aataacccgc gcttaaggag aatgtaccta acatgatacg gcgttttact tcttcaccga
    29581 atccttctga acgtgagttg acatatacat catctaatga tttcacattt tcagagcgat
    29641 aaccgtagcg gataccatcg aatcgttgca aattggaact tgcttctgat gaggcaatga
    29701 tataataaac agccacaccg tattttgaat gaggcaagct gacttcttcc acaacagcac
    29761 ctaatgaacg gaatgtttct gctgctttca atacagcttc ttttacgcca ggggccacac
    29821 cttcgcctaa gtattcttta ggtaaagcaa tcttcattcc tttgatgtcg cctgtcagtc
    29881 cctctgcaaa atcaggaact gaaacgcctg aagaagtacc gtctttttcg tcataaccgc
    29941 tgatagctgt caatgctaat gcattatctt tcaccgtacg ggtcattggt ccgatttggt
    30001 caagagatga agcaaaggca atcaaaccaa aacgtgagac acggccataa gtaggtttca
    30061 tccctacgat gccgttaaaa gctgctggtt ggcggatact tcccccagta tcactaccta
    30121 aagaaactgg aatttgtcca gctgcaactg ctgctgcaga accaccagaa gagccaccag
    30181 gtacttttgt tgggtcccaa gcattttttg ttttcttgaa ataagaagtt tctgtacttc
    30241 cacccatagc aaattcatcc atgtttagct taccaacagg gatcatatct gcttgataga
    30301 ttttatccat gaccgttgca tcataaattg gttcaaaatt gtaaaggatc ttagatgcag
    30361 cggttgtcaa gatatctttt gttacgatat tatccttgat accaattgga ataccagcaa
    30421 ggacgttgct ttcatcaatc cctttttcat ctaaagcttt tgcctgttcc aatgcttttt
    30481 cttcgttcaa tgtaatgaat gcctcgacct caggctctgt ttctttgatt cgttcaaatg
    30541 ttgtttttgt caaatcttgt acagtgattt ctttcgatac aagcatatga tgcagttctt
    30601 ctagagaacg attgtgtaat tctgtcatta tgcgccagcc tccccattat cgatgattgc
    30661 gggtactttg ataaagccgt cttctgtttc cggcacattt ttcaataatt catcgcggtt
    30721 ccatccagct tgcgcccgat cttgtcgcat cacgtttact gtttcaacca cattcgacgt
    30781 aaatggaaca ccttccgtat ctacttcttc cagcatttca accatatcga tgattttacc
    30841 taattggtcg gtaaaacttg caagttcttc atctgcgaac gcaagttttg ctaatttggc
    30901 aacgtgtttc acttgttctt cactaatcgc catactaccc cttctttcta aacgagttaa
    30961 aaactttgct ctctatttta acacaagagt gccgtgtctg aaattgttta ttgaaatttg
    31021 tctgaaaaaa ctcatttttc attcttctac tgagcgactt ttagccaatg catctgccat
    31081 ttgatcttct gtccaaactt cgattcctaa ttcttgtgct ttggtcagct tgctgccagc
    31141 atcttctcct gctaccacga tatccgtttt tttcgatacg ctaccagtca ctttcccgcc
    31201 aagattttcg atcgtctctt ttgcttcttc acgagtaaat cgtgttagtt taccagtaag
    31261 gactactgtt ttttctttaa aaggagactc gacttcttgc aattgggcat ttcgaagccc
    31321 tttgtattcg aaattgacgc ctgcttgttt taattcattc atcaattcat ggacttcttc
    31381 gttactgaaa taagtgacta cactgtcggc aatgatatca ccgatcgtat caagcgcaat
    31441 aatcgactca tagtcacttt tactcaatgt ttctagatca ccgaaatgtt ctgctaaaat
    31501 ttttgctgct ttcgctccga catgtcggat tcctaacccg aaaatcaacc gctcgaccga
    31561 attatctttg ctgttgtcga ttgctgtcaa aatattattt gctgattttt cttttatttt
    31621 gtccaacgtc aataactctt cttctgtcaa tttgtaaaga tcagcaacat ctgctactag
    31681 ttttttatca tacatctgtt ctagtacgcg cggccccaac ccatcgatat tcatcgcatt
    31741 tcttgaaaca aagtggttca aaccttcttt catttgagca gggcatttag gattgatgca
    31801 ccgtaaggct acttcttcat ccaaatggac aagttcactt ccacaaactg gacaatgtgt
    31861 aggcaattgg tattcttcgc tgtctttagg tcgtttatct aacacgactt gcgaaacttc
    31921 tgggatgatg tctcccgctt tataaatcag aactgtatct ttcaagcgga tatccttcaa
    31981 cttaatataa tcaccgttat gcaggctggc acggcttact gtcgttcctg ctactcggac
    32041 tggtgtcatg atagctgttg gcgttactac ccctgttcga ccgactgtcc attcgatgtt
    32101 ttcgattagc gtttcaactt cttctggcgg aaacttgtaa gctgctgccc atctcggtgc
    32161 tttgatagta aaaccaagtt gatcttgtag cgagaattca ttcactttga tgacgattcc
    32221 atcgatttca tagggcaaat ccacacgttt gtcatgatat tcttcaatgt atgaccacac
    32281 ttcatcaatc gaatgacata gacgtttttc ccgattcgtg tggaatccaa ttttttctag
    32341 ctcttctagc gcatcatact gggtttttgc ttgcatagga ccgaaatcag ctactgtgta
    32401 taaaaaagtg tccagatttc ttttcgctgt gattttagaa tctaactgac gcaaagaacc
    32461 agctgcggcg ttccgaggat tcgcaaagat atcttttccc tctgcttctc tgtcttggtt
    32521 caattggaca aatgaccgtt ttggcatgaa acattcgcct cttacttcga tgttcattgg
    32581 ttcttttaat ttgattggga ccgaccggac agtttttagg ttttcagtga tgttttctcc
    32641 tacagttcca tccccgcgag tcgcacctct cacgaaattt ccatcttcat aacgcaaaga
    32701 aacggaaagt ccatctattt ttagttcaca gcaatagtca actacacgtc ccactgcttt
    32761 ttgtacacgt tgatcaaaag ctatcagctc ttctttgctg aaaacgtcgt tcaaactgta
    32821 aagcggaata tcatgagtga ctttttcaaa accttctaat acttttccgc ctactcgttg
    32881 agtgggagaa tcagacgtga tcaaatcagg atattgttcc tcgattgcta ctaattctgc
    32941 gtattcttta tcataaacat agtcttccac tgttggttta tcttctacgt agtattcgcg
    33001 agaccattgg ttcagtcttg tacgaagttc ttctgctcgt ttagcagctt cgtcaatcgt
    33061 catttcatgt tccatcattt accctccact actaagcttt ttcaattggc gcaaaggcag
    33121 ccaaaagacg ttttactcct tgacttggga aagccacatc tagttctaga tccttcgacg
    33181 tcccgcttac tcggacgact gttcctagac cccatttttt gtgtttgact ttatctccag
    33241 gtttccagct gttattttcc cctccagtag cacttttaga gggaacaacc gctgtctctg
    33301 gctgtttata gctatatgct gtttttttag cgaatacagg cgttttttta ggcgtaggac
    33361 gcatgccttc gatttccagt aactcctgat cgatttcttc tacaaaacga gaaggtcggt
    33421 tatattgcgt ccgtccatat aatgttctgg agaaagcatt tgttaaatac aacgcttctt
    33481 ctgctcgcgt gatccccaca tatgcgagac ggcgttcttc ctccagttcg ctttcttcca
    33541 tcaatgctcg agataaagga aaaaccccct cttctagacc aatcaggaat acaactggaa
    33601 attctaatcc tttggctgca tgcaaagtca tcaatgtcac ttgggaagca tcttcttcta
    33661 aattgtcaat gtcggaaaca agtgccagat catttaagaa gaccgttagt ttttcttctg
    33721 gtgcatcagc gtcttcttca ttttgctgtt cgaattgttt gtcgaattct tgtgtgaccg
    33781 ttaaaaattc ctccaagttt tcgatgcgtg cttgtgcttc taatgtattt tggattttta
    33841 agtcttctag atatccgcta cgatccaaaa cctctttcgt caattctgtc actgtcaagt
    33901 atgggatcat ctgagtgact tcttggatca tctctccaaa tgcacctaat tgctgtcctg
    33961 cttttccacc gatatttgcc agatccacat tttgcgctgc ttcaagcagt ggccattcat
    34021 gaagagaagc aaaactgcgt agtttttcga tagatcctgg tccgatgcct cgtttgggcg
    34081 aattaacgat acgctcaaaa ctgattgaat cttgaggatt taccagcaca ttcaaatacg
    34141 ctaaaatatc tttgatttct ttacgatcgt agaacttgtg ccctccaacc attttataag
    34201 gaatatttgc tttcaacaga gtttcttcca ttacacgaga ttgtgcgttc gtccggtaca
    34261 agatggcaaa gtcgccgtaa ttccgatgat tgcttcgaat ctcttcttgc atccggtcta
    34321 cgataaaacg ggtttcgtct cgttcattat ctgcacggta gtaagtgatc ttgtttcctt
    34381 ctttgttttc tgtccacaag ttttttggtt ttcgattgct attgttttca atgacttggt
    34441 ttgctgcgct aaggatgttt ttcgtggagc gatagttttg ttctagcaaa attactgcag
    34501 catctggata atctttttca aaatccaaga tgttctgcat atctgcaccg cgccagccgt
    34561 aaatactttg atctgcatct cccacgacgc acaggttcct aaatctacct gccaataaat
    34621 tgactagcgt atactgtgcg tgatttgtat cttggtactc atctacatga atataatgga
    34681 atttgttttg ataatacgtg agtgaatcag gatgttcttc aaataatcga atcgtattca
    34741 tgatcaggtc gtcaaagtcc atacattgat tatattgaag ctctttttgg taagcagcat
    34801 aacattttgc cgcaatctct tcgtaataac tgccttgcat ttttgcaaaa tcctgagggg
    34861 tctgcaggct gttttttgct tgagagattg ttcctaatat acttcgagga tcatattttt
    34921 tcggatcaat gttcagttct tttagtatcc gtttcatcaa ggtcagctgt tcagaagaat
    34981 cgatgattgt aaagttccgg ttatacccga taaaatcaac atctcttctt aaaatacgca
    35041 cacacatcga gtgaaaagtc gatacccaga catcttcgcc tccagatgcg agaatcgcat
    35101 tgacacgttc tttcatttct tttgcagcct tattggtaaa agtaatcgct aggatgttcc
    35161 aaggattgac ttctttttcc tcaatcaaat aggcaatgcg gtgtgtcagc actctcgtct
    35221 ttccacttcc tgcacctgcc attagcagta atggtccgtc agtgtgaaga acagcttctt
    35281 tctgacgggg gttcatcccg tttaataatt cagctttgga tgacattaac aacatccttt
    35341 cttttatcaa tcttgttaat tatagcattt tcttggcttt cattaaacta gctagttcaa
    35401 aatcatttgt ttctttttat ggatttttcc ttgtcaattc gttgatttcg cgctaaatta
    35461 aaagaaagcg aggcgtttga atggaaaaaa aaggaaaaat caagaaatta gataagactc
    35521 agatatattt tattctaaaa ggaatatttg ttggactgct gacaggaatc gtcgttagcc
    35581 tgtttcgatt atctatcgaa aagctaagcg atttagttcg aagtatctat gaaatgagtc
    35641 gggaacagcc gatttactta attggcctag ctatttgctg catccttgca gcatttttcg
    35701 tgggttatct ggtcaaaaat gaaccggata tcaagggaag cgggattcca caagtagtgg
    35761 gacagcttcg cggggaatta tcaatgaact ggttctctgt tttatggaaa aagttcattg
    35821 gcggtgtact ttcagttggt gcgggccttt ttctaggtag agaaggacct tctatacagc
    35881 ttggcgcatc cattggacaa ggagctggac aattgtttcg gagtccatct tcagaagaaa
    35941 agattttaat ttctagcggt gcaagtagcg gacttgcagc agcattcaat gctcctatcg
    36001 caggattact ctttgtttta gaagaaatcc atcactcctt ttctccactt gtttggctga
    36061 catcatttgc ttcagcaatc actgctaact tcgtctctct ttactttttc ggcttgagac
    36121 ctgttttata tctaggtgac cttccttctt tgccgttgaa atattatggt tccttagtga
    36181 tcttaggagt acttctaggc atcttaggtt ttatttatca gaatgtcttg ctggctttgc
    36241 caaaatttta caaccgattg cctttaccag cttattttca cgggttcgtt cctttcttgc
    36301 tgattttgcc aattggttat ttatggccta gtactctagg cggaggcaat caaatcgttt
    36361 tagcttttgg acatgcttcc ttgccaatca ttatgattat cggtttgttt gttcttcgat
    36421 tcgttttttc catgatttct tacggtgggg gtcttccagg ggggattttc cttccgatat
    36481 tgacattggg ggctttaatc ggtagctttt atggaaacat tatggttgat ttcgggatgg
    36541 atccaatcta catcaaggat tttgtcgtta ttgccatggc tggttatttc acagcgattg
    36601 gaaaagcacc gctgacagca attattttag tgacagaaat ggttggctct cttaatcatc
    36661 tgatgccttt aggattggct gctcttgttg cttatatcgt caatgacctt ctcggcggta
    36721 atccaattta cgaatcatta ttggaacgtc tacttggaaa tactcagacc gcctcactaa
    36781 aaggtgcaag gacaactttt gattttccag taacggcaga gagttcttta gacggattaa
    36841 tggtacgaga cttcaattgg cctaaaaata tgctgctaat ctctatacgc agaggaaatc
    36901 aggaaatctt gactcacggt gatacgatca tgaatgtagg agatattttg gttatcttga
    36961 ctgatgaagc aaacttgcca agaatcaagc aggaaatcga ggctaaatct tctatcacaa
    37021 atttgaagca atcattttaa cgaacatgga aaaactattt tacaacaact attcatctac
    37081 actacttgat tctatatgat tctataaaaa aagaagaggc tgggaaaacc cagcct
//
LOCUS       DAZKOB010000016        34999 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000016 DAZKOB010000000
VERSION     DAZKOB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.016"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            193..1899
                     /gene="efbA"
                     /locus_tag="ACG8M6_000832"
     CDS             193..1899
                     /gene="efbA"
                     /locus_tag="ACG8M6_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0456521.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2057..2959)
                     /locus_tag="ACG8M6_000833"
     CDS             complement(2057..2959)
                     /locus_tag="ACG8M6_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0456522.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2956..3648)
                     /locus_tag="ACG8M6_000834"
     CDS             complement(2956..3648)
                     /locus_tag="ACG8M6_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0456523.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3691..4332)
                     /locus_tag="ACG8M6_000835"
     CDS             complement(3691..4332)
                     /locus_tag="ACG8M6_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0456524.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4370..4684)
                     /locus_tag="ACG8M6_000836"
     CDS             complement(4370..4684)
                     /locus_tag="ACG8M6_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456525.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4672..5301)
                     /gene="pyrE"
                     /locus_tag="ACG8M6_000837"
     CDS             complement(4672..5301)
                     /gene="pyrE"
                     /locus_tag="ACG8M6_000837"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0456526.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5308..6024)
                     /gene="pyrF"
                     /locus_tag="ACG8M6_000838"
     CDS             complement(5308..6024)
                     /gene="pyrF"
                     /locus_tag="ACG8M6_000838"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0456527.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(6021..6953)
                     /locus_tag="ACG8M6_000839"
     CDS             complement(6021..6953)
                     /locus_tag="ACG8M6_000839"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0456528.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6950..7726)
                     /locus_tag="ACG8M6_000840"
     CDS             complement(6950..7726)
                     /locus_tag="ACG8M6_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0456529.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7780..10965)
                     /gene="carB"
                     /locus_tag="ACG8M6_000841"
     CDS             complement(7780..10965)
                     /gene="carB"
                     /locus_tag="ACG8M6_000841"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0456530.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10970..12046)
                     /locus_tag="ACG8M6_000842"
     CDS             complement(10970..12046)
                     /locus_tag="ACG8M6_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0456531.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12051..13331)
                     /locus_tag="ACG8M6_000843"
     CDS             complement(12051..13331)
                     /locus_tag="ACG8M6_000843"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0456532.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13350..14276)
                     /locus_tag="ACG8M6_000844"
     CDS             complement(13350..14276)
                     /locus_tag="ACG8M6_000844"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0456533.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14289..15572)
                     /locus_tag="ACG8M6_000845"
     CDS             complement(14289..15572)
                     /locus_tag="ACG8M6_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0456534.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15586..16125)
                     /gene="pyrR"
                     /locus_tag="ACG8M6_000846"
     CDS             complement(15586..16125)
                     /gene="pyrR"
                     /locus_tag="ACG8M6_000846"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0456535.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16360..17271)
                     /locus_tag="ACG8M6_000847"
     CDS             complement(16360..17271)
                     /locus_tag="ACG8M6_000847"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0456536.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17268..17732)
                     /gene="lspA"
                     /locus_tag="ACG8M6_000848"
     CDS             complement(17268..17732)
                     /gene="lspA"
                     /locus_tag="ACG8M6_000848"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0456537.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17737..18435)
                     /locus_tag="ACG8M6_000849"
     CDS             complement(17737..18435)
                     /locus_tag="ACG8M6_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0456538.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18422..18808)
                     /locus_tag="ACG8M6_000850"
     CDS             complement(18422..18808)
                     /locus_tag="ACG8M6_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456539.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18955..20625)
                     /locus_tag="ACG8M6_000851"
     CDS             complement(18955..20625)
                     /locus_tag="ACG8M6_000851"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0456540.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20808..21041
                     /locus_tag="ACG8M6_000852"
     CDS             20808..21041
                     /locus_tag="ACG8M6_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0456541.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21140..21571)
                     /locus_tag="ACG8M6_000853"
     CDS             complement(21140..21571)
                     /locus_tag="ACG8M6_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0456542.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21650..22063
                     /locus_tag="ACG8M6_000854"
     CDS             21650..22063
                     /locus_tag="ACG8M6_000854"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0456543.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22101..23249)
                     /locus_tag="ACG8M6_000855"
     CDS             complement(22101..23249)
                     /locus_tag="ACG8M6_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0456544.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23371..23967)
                     /locus_tag="ACG8M6_000856"
     CDS             complement(23371..23967)
                     /locus_tag="ACG8M6_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0456545.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24167..26809)
                     /gene="alaS"
                     /locus_tag="ACG8M6_000857"
     CDS             complement(24167..26809)
                     /gene="alaS"
                     /locus_tag="ACG8M6_000857"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0456546.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27197..27628
                     /locus_tag="ACG8M6_000858"
     CDS             27197..27628
                     /locus_tag="ACG8M6_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0456547.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27673..29022)
                     /locus_tag="ACG8M6_000859"
     CDS             complement(27673..29022)
                     /locus_tag="ACG8M6_000859"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0456548.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29092..30255)
                     /locus_tag="ACG8M6_000860"
     CDS             complement(29092..30255)
                     /locus_tag="ACG8M6_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0456549.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30384..30707
                     /locus_tag="ACG8M6_000861"
     CDS             30384..30707
                     /locus_tag="ACG8M6_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456550.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30890..32239)
                     /locus_tag="ACG8M6_000862"
     CDS             complement(30890..32239)
                     /locus_tag="ACG8M6_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0456551.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32308..33261)
                     /locus_tag="ACG8M6_000863"
     CDS             complement(32308..33261)
                     /locus_tag="ACG8M6_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0456552.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33363..34553)
                     /locus_tag="ACG8M6_000864"
     CDS             complement(33363..34553)
                     /locus_tag="ACG8M6_000864"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0456553.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34753..>34999)
                     /locus_tag="ACG8M6_000865"
     CDS             complement(34753..>34999)
                     /locus_tag="ACG8M6_000865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456554.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9961 a         7648 c         6125 g        11265 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgatacc taaaaatttg gatttcaacg
       61 atagaaacac tgttttttat gtaattagtc caagattttt ctagactttt gctttactat
      121 tcggccaaat tggtttttct tctttttttc gttataatga atatacttat gaaaagaaat
      181 gaggaatgaa ccatgtcctt tgatggtgta tttacccacg cgatggtcaa cgaattgcgt
      241 gaaacgttac tatctggccg gatctcgaaa atccatcagc cttatgaaaa tgaaatcgtg
      301 ctggtgattc gttcacgcgg caaaaaccat cgattattat tatctgcgca cccaagttat
      361 gctcgtgtac agattaccca aatcgattat cagaatcctg ataatccgcc taacttcgtg
      421 atgatgcttc gaaaatattt ggatggcgct attttagaag acatcgaaca aatcgaaaat
      481 gatcgagtga tccatttcca ctttgctaaa cgaaacgagt tgggtgatct acaaaatatc
      541 atattgatcg ttgaattaat gggacgtcat agtacgatcg tcttagttaa tcgagaaact
      601 ggaaaaatac tggatgcgat caaacacatt ggaagctctc aaaacactta tcgttctttg
      661 cttcctggtg tagaatatgt tgctccgcca aaacaagagg tcttgaaccc atttagctcc
      721 gagaaagaaa aaatctttca gcggctttcc caaactgaac tagatccaaa agcaatccaa
      781 cgtcagtttc agggaatcgg tttcgatatc gctcaagagc tgaccaaaag attactggaa
      841 cgtcctaatg aaaagatggt tgtatgggac gaatttttct cagccatcag ccatcagcct
      901 attcctactt tttacgaaac ggaaaataaa gattttttca caccgattgc ttatcaggta
      961 ttgtccgaac aggcatctgc tgtaacagct tatccaacac tcagtcaatt attagatagc
     1021 tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac aaggcggaga actgattcga
     1081 aaaatcgaaa atgaactaaa acgaaataaa aataaattga aaaaacgtga acaaacactc
     1141 aaagaatccg aaaacgctga gaattaccgt agaaatggcg aactacttac tacttttctg
     1201 acacaagtac cacgcggcgc aaaagaagtt gttttaccta attattatga agaagatcgg
     1261 ccaatcaaaa tagcactaga tccggcattg acaccaaatc aaaatgcaca gaaatatttc
     1321 catcgatacc aaaagctgaa gaacgctgtt aagctgattg gcgaacaaat ccaagaagcc
     1381 aaagatgaaa tccaatatct tgaatctgtc ttatctcaac tggaaattgc cggcccaatg
     1441 gatatcgaag caattaaaga agaattgact tcagaaggat acctgaaaaa gaagaactta
     1501 aaaaaacaaa aacgtaagaa accatctcag cctgatcaat acttttcttc tgacggaacg
     1561 ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc tgacaatgaa gacagctaaa
     1621 aaaaccgact attggcttca tgcaaaaaat atacccggtt cgcacgtcat cattaaaagt
     1681 gacaagccct cagatgaaac aattacagaa gctgccgagt tagctgccta tttttcaaaa
     1741 taccgatact ctgcccaagt gccggttgat cttgtacagg taaagcatat ccgaaaacca
     1801 aatggcgcca agcctggtta tgttatttat gaaaaccaaa agacgattat cgtgacacca
     1861 gaagaagaaa aaattacagc tatgaaacaa aacaggtaat caatgagtgt ttctagaatc
     1921 atatttgcgt actttcccac aacagcaatt agttataaat tgtttgctac aaaaagagga
     1981 gttgtgacaa aattctgtca caacccctct tttttgatca acggtgttca ataaacagcg
     2041 ttctcatctg cttttcttag ctctgctggt ccagtaaccg agtaagctct ttgataaatc
     2101 tttgagcgat ttcactgagc ggacgtttcg ccgaataaat gatataaaat tcatttagtt
     2161 cgccttctgc caaagggatt aaccgcaagt gctcgcgaat ccctccaatc acaataccag
     2221 atcctgaagc gtaagcatca gtttcaagaa cgatattcat caaagtacct cgatcactcg
     2281 tataaatcac tgtttcttga tcagggatct cgataaggtc ttcataaaaa tacgggaaat
     2341 tatttccatc ctgtgtaaaa cgaacttgag gataagataa caattcttct ttggtgatct
     2401 ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat atgcgttttg aattttccaa
     2461 tgctgcggcc acttagctct ccttgttgta aatatcttct gataccagct tgattatgct
     2521 cattcatata aataatgccg atctcactgt gctgctcttt gacttcttcg atcactctcg
     2581 ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc tttgaattcc tcgagtagct
     2641 gtgcggtcaa aggacctaag aaatcataat gctgggcagc gatcgaaaaa ttgtctctct
     2701 tttcactcgt tagatagtga gtttctaatg tatcaagctg gaccaatatt ttctcggcac
     2761 gatttaagaa gtcttgccct tcttctgtta aatatgcccc tcgattcgtt cgagtaaaca
     2821 gcgtgatccc taactcggtt tctaattctt tgataccagc tgaaagactt ggctgggaga
     2881 caaacatttt ctttgccgct tctctgaaac taccattgtt tgctacagcg accacgtatt
     2941 tcatctgctg aatattcatt gctcaaactc ctcgaaataa taaatttctc ttccattcaa
     3001 gggctggatt ggtcggttgt tcggtcgagc atactgacca tcgaagggga gaagaaactc
     3061 ttggttcagt tttcctacat gatccattac aaaccattgg attggcccgc tggtaggcgg
     3121 agttgtcaat gagccaatat aatggaaatg gcttttttgc tccggaaaga acacatccgg
     3181 atttacccaa tgctcatgtt tttctaagtt ccaactttcc ccattttctt gattatacag
     3241 ccaaccttcc tgtaccatta gctggtacat gacacccaca actagattct cttttttgct
     3301 gttcatatga acaaaatgaa attcaatatc ttcctgtttt ccgttgatga tatgttcaga
     3361 aggcaagtgg aaatgaatat ctgtcaaata atagcgttct ttcttgaact ctacataact
     3421 gagttggtca aaaggtacta aatgaatcgt gtttttgaat tccttgtcag taaaccgttg
     3481 cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg gtttcttccc ccttggtcaa
     3541 atgaataggc gactgcaatg gaaattccgc acctctcgca aaccaatcac ataaactcgc
     3601 ccaattttcc ggacctttgt ctccagaata gccccattcg acatccatat tctttttcaa
     3661 ctatacttcc ccctgcatct catatcattt ttatttttct tctttcttta caatcgttga
     3721 gacggtttct aaaaacgcct cttttccata aaattcctgg ttcaatagtt ccggaaatat
     3781 ctctttaaag aaatactgaa cagaaggccg atgttcaaag cttttaatcg tagtcactgc
     3841 ctcaatgaac atctccttat aaactttttc cgggttattt ttgatgattt catcaaaaga
     3901 ttcataaaga aggtcaacct tatctgaaat ggctaatagt tctccttcta cggtttcatc
     3961 ttttccttcg aacaaccgac ggcgataaac ttcttggaac tcagctggaa tctcttggtt
     4021 gatgaattcg tccgtcatct tttcttcaac tgtttgcagc atccctctta actctttgct
     4081 tgcgtatttc accggcgttt tgatatcacc aataaaacgc tctgtgtagt catgattcaa
     4141 tgatttttca tagagcagtt tccaatcgat cggttgtcca ttcgcttctt caatgtctcc
     4201 tagaacttgc gcaatagatg ctacacgata cgaatgtgct gcaactgtat gctctgtata
     4261 tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg ttcaatccta aaataaattc
     4321 attcaacccc ataaaagtta cctccctttg agatttcttt ttccgaatat catatcatta
     4381 aataactgat attcgcaaca tttccgctct ttaccatttt tgaaacagct cccttaccaa
     4441 aaaatagaga aaatctatat ataatagaaa aaagagaaga gattattctc ttctttctgc
     4501 taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa ctgatcacga tagaagtttc
     4561 ttttcatacc tgatgaaaag atgcttctcg caatcagttt ttggattttt gctattccaa
     4621 ttgatgattc tggagacaca aatcggattt ctctatcttc atcaagcgcg cttaaatctg
     4681 ccaattttca ggatcttttt tccattcttt tagcagagtt aattcttgct catcgatata
     4741 acgatcttct aaagctgctt caatcaatac agaataattg gtcaacgtca taagagggat
     4801 ttcggctttt tcgaaatttg cttttccttt tggcaactca tacgtaaaga tagccgcaac
     4861 accaaggata tccgcacctt ctctttttgc tgcttcagct gcttccaaga cacttccgcc
     4921 tgtagaaatc aaatcctcga tcacaaccat cttctgacct tcaaatatac gtccttcgat
     4981 ttggttacct ttcccatgat ctttcgcctt acttcgaata tagaccattg gcaaatccag
     5041 tatttccgct acccatgcgg catgagggat tcctgctgtt gcagttccag caatcacttg
     5101 tacttcaggg taagcttctt tgattttgct tgctaatcct tttgcgatct cttttcggac
     5161 ttttggatag ctcatcgtga ttcggttatc acagtaaatt gggcttttga ttccacttgc
     5221 ccatgtaaaa ggattacttg gatttaaaaa tactgcttca atttctaata agtcttttgc
     5281 gatactttgt tcaacattca ttttaggtta gctccattct gtttgaatcg tttgatatgc
     5341 tttataagga ttagttgact gagtgattgg tcgacctata acgataaaag ttgagccgat
     5401 cttttttgcc tgagaaggtg tgactactct tgtctgatct cctgcgactg ttccttctgg
     5461 tcttataccc ggagttaagc aaacaaattc gtctcctgcc atttctttga tcgcttctac
     5521 ttcccaagca gaagaaacca caccatctaa tcctgctttt ttggcacagc tagcataatg
     5581 gatcacactt ttttctaaag aaacttcgat tagttgatcg gcatgcattt cttcttcact
     5641 tgtagaagta agctgagtga tagctagaag tagcggacgt tttccacctt ctggagtccc
     5701 ctcttctaat cctcgcattg ccgcctccat catacgaata ccgccagcgg catgaacaca
     5761 ggtgagatca acaccaagct ttgccaatcc ccgcattgct ttttcgaccg tattcggaat
     5821 atcgtgaagt ttcagatcta aaaatacatt atgaccagcc tcttttagat aacgtacgat
     5881 ttcaggtcct tcttggtaga acaattccat tccgattttc acaaacaatt tttcttcttt
     5941 cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt gaaaaatcta atgcaataat
     6001 tggtcgttgg tctagttgat tcatcgttca cgttcctctc tcacttcttt gattaatctt
     6061 tccagcgatt cgatgcctag ttcgctcatt cgcacaggga gctctttaat tagtttagga
     6121 cagatatacg gatcggtgaa attcgcggtc cctatagcta cagcacttgc tccagccata
     6181 aacatctcaa gaacatcatc cactgtctgc acgccgccca tcccaatgat tggaaggctg
     6241 gatatacttg ctacttggtg aatcaaacga atagcgacag gcttgattgc tggtccggag
     6301 agtccacctg tttgattggc taaaatcggt tttcttgtct taaggtcaat ccgcatacca
     6361 agaagagtat tgatcatcgt aaacccatct gcccctccag cttcgattgc ctgagcgatt
     6421 ggaacgatgt ctgttacatt tggggacaat ttgacataga caggaacaga agaaactttc
     6481 tttactgctt gtgttaattg aaaagcaaca tcagaatctg ttccaaaagc aattccgcca
     6541 tgtttgacat ttgggcaaga aatgttcagc tcgattgctt tcacatttgg tgcttcgcca
     6601 attttgctac aaacttctac atagtcttct tcacaagcac cagcaacatt tgcgatgatg
     6661 ggtaactcat tgtactcctc taattctgga agaagtttat gcataacaac gtctagacca
     6721 ggattttgca aaccaattgc attcaacatg ccagatggtg tttccgccac tcttggcgtg
     6781 ggattgccaa agcgggcatt tggagtcgtt gccttgacca tgatcgatcc aagctgattc
     6841 aaatcatagt attttccgta ttctttgcca aaaccgaaac agccgcttgc cggcatgata
     6901 ggatttttta attctaggcc aggaagtttg accgctaaag gattctcatt catataatta
     6961 cctctcctat tttaaaaatc ggtccttcgt cacaaacttt cacacttttt gttcctgttt
     7021 catcttccgg cgtatgacaa acgcaagcat agcaagcccc tatcccacat gccatgcggg
     7081 attctaatga taactggaca ttttgatgcg aagaaaacag ctgttccact gtcttcaata
     7141 acccgtttga accacatgca aaaacagctg taggttccgt cttcgttcct aacaataaat
     7201 taccgacatt tccgtgcata ccgtatgttc catcgtcagt agcgattctt gtttctccta
     7261 gacgattaaa ttctttttca taataaacga cttcatgtgt ggcaaaacct aaaaaatgcg
     7321 ttggcttaat tcctttttcg actaactgtt ttgataactc atatagagga gggacaccga
     7381 ttcctccacc gattataaat acttcgtctc ctgtatgaag attggacaaa tcaaaaccat
     7441 tgcctaaagg acccatgaca tctaaatggt ctccggcaga aagagtagag aagactctag
     7501 tcccctcacc ttccacccga taaatgatac ggcaagtatt tgtttcgtgg tcgatatgat
     7561 tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg cacgagaata tgcaagaact
     7621 gtcctggttg ctgcatttct tttactagat cacctttcaa tgtcatttca aaaatctttg
     7681 gtgccagctg tttttgctga acgatttcca tcatttcttg tttcatctgt cccctcctgc
     7741 aacggttttt cgtctttttg tcttaacatg tgctcgccgt taaatagggt tagttgaaaa
     7801 agcacgggat tccatcactc gaacgatcgc atctgctgtg tctaaagaag taaacaatgg
     7861 aataccatgt tcaactgctt ctcttcggat gatgaaaccg tctttagaag catttccccg
     7921 atctttatca atcgtattga caaccacttg tgttttacca tctcttatta agtcgactac
     7981 atttttttct gctgcttcct cagaaatttt cgctactgtt tcaacgacca gaccattttt
     8041 ttcgaaataa gcagctgtat gtttcgttcc tatcagacta tagccaattt ctgtaaaacg
     8101 tttagctaat tctagtgctt cttcttttgt ttcgtccgca atcgtaaata atacggctcc
     8161 atattcaggc aagtgtaatc cagaagcttc gaatgccttg tacaaagctt tgtctaattg
     8221 tctgtcagat cccattactt ctccagtcga tttcatttca ggccctaagt aagtatcaac
     8281 tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca tgaacttctt ttgtttctgg
     8341 ataaagtccg ttttcataac caagtgtctt tagtttctct cccaaaatag ctttcgtcgc
     8401 aatttgtgcc attggaattc ctgtcacctt acttaagaat ggaacagtcc gacttgctcg
     8461 aggattgact tcgatcacat aaacctcatt ttcatggatg acaaactgga tattcatcat
     8521 cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa tcttcgatcg ttttcttgat
     8581 cgattcagat aatgtttgag gtggatacac agccattgaa tcccctgaat ggacaccagc
     8641 acgctcaata tgctccatga tccctgggat gagtactgtt tcaccgtcac aaatcgcatc
     8701 cacttcgcat tcaccaccaa gcaagtaacg gtccactagt actggatgtt ctggagatgc
     8761 tttcacagca tgttccatgt aatcttctaa atctcgctgg ttttcaacga tttccatcgc
     8821 acgtccgcct aatacatagc ttggacgaac aagaactgga taaccaattc tcgaagcgat
     8881 aaccacggct tcttctttac ttgtagcagt atcccctaaa ggttgaggaa tatccaactc
     8941 tttcagcgct tgctcgaata aatcacgatt ttccgcacga tccaagtctt cgatcgttgt
     9001 ccctaagatt tttacaccag cagcagtcaa aggttcggct aagttgattg ctgtctgacc
     9061 accaaattga acgattactc ctatcggctg ttctagctcg atcacattca tcacatcttc
     9121 gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa aagtcagtcg aaactgtttc
     9181 aggattgctg ttcatgatga ttgcttcgta tcctgcctgc tggattgctt tgacagaatg
     9241 cacagtcgca tagtcaaatt caaccccttg tccgatacgg ataggaccag atcccagtac
     9301 tagaattgag ggcttttctg atcgtgaact ttcattttca tattcatagg aactataaaa
     9361 ataaggtgtt tttgattcga attccgctgc acaagtgtca accatcttat atacaggaat
     9421 caaatcattt tcttttctca tctggcgaac ggcttcttcg tttatttccc acagctccgc
     9481 cacttttttg tctgtaaaac cgttttgttt cgctgttttc aatatcttgt catccaacgg
     9541 ctgctgtttc aactgctgtt cgatttctac gatatgcaac agtttatcta aaaagaataa
     9601 atcgattttt gtcaaatccg ctaattcttc tatggtgtac cctcttctaa tagcttcagc
     9661 tacataaaac aggcgatcat cttgtgctcg aaccactttt tggattaggt catcgtccgt
     9721 gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg atttctaaag aacgcacagc
     9781 tttcaataaa gactcttcga tattccgacc gatagccatc acttcaccag ttgctttcat
     9841 ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca aacggccagc gaggaatttt
     9901 agctacaaca taatctaatg caggttcaaa ttctgcaaaa gtcgttcctg taacaggatt
     9961 tttcatttcg tctaatgtta atccaagcgc tattttagca gctagcttag cgatcggata
    10021 gccagtcgcc ttacttgcta acgcagatga acgagaaaca cgcggattga cttcaatcac
    10081 ataataatta aaactatgtg gatcaagagc gagttggaca ttacagcctc cctcgatctt
    10141 aagtgctcga ataatcgaaa gagaagcatc acgaagcatt tgatattcat ggtcagacaa
    10201 tgtttgacta ggggcaaata cgatagaatc acctgtatgg atacccaccg ggtcaaaatt
    10261 ttccatattg cagacaacga ttgcattatc agccgaatct ctcatcactt catattcaat
    10321 ttctttaaaa cctgcaatac ttcgttcaat caaacattgg gtagcggggg aaagacttaa
    10381 tccattttct gatacgatac gtaattcttc ttcattttca cacatacctc caccagttcc
    10441 gcctaaagta aaggctggac gaacgatgac aggatagccg attttatttg caaaatctac
    10501 agcttcttct actgtattga cgatttcaga ctcggggatt ggctgattca attctttcat
    10561 caactgtttg aatagatcac gatcttctgc ttgatcgatc gcagaaagct ttgttcctag
    10621 aagttctatg ttcaattctt ctaaaatacc tgatgctgcc agctccattg ccatattgag
    10681 gcctgtctgt ccgcctaaag ttggaaggag agcatctgga cgctcttttc gaagaatcct
    10741 tgatacaaat tcaagtgtga tcggttcgat atatacttga tctgccactt ctttatccgt
    10801 catgatcgtc gcaggattag aatttactaa aaccacttca tatccttctt cttttaaggc
    10861 aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct tgaccaatga tgatcggacc
    10921 agatccaatc accataattt tcttgatatc tgtacgtttc ggcatctgtt tactgctcct
    10981 ttcctgcgtc catcatttcc ataaattcat caaataaatg tagtgcatca tgcggaccag
    11041 gggcagcatc tggatgaaac tgcacactga aagcgggata atctctgtgt ttgactcctt
    11101 ctactgttcc gtcatttact tctacatgag ttacgatgag tttttctgga tcaatcgtgg
    11161 cttcatcgac tgcatagcca tgattttgag aagtaaaatc aattcttccg gtcgctactt
    11221 ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat tttataggta tctgcaccgt
    11281 tggctaaagc aaataactgg tgaccgagac aaataccaaa aatcggtact tttccttgga
    11341 tttttttgat catttcgatt gcttctggca catcttttgg atcaccaggt ccattcgtca
    11401 gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc atctgtgtta tagggaagta
    11461 ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat actatgtttc aagccgaagt
    11521 caatgacaat gactttccga cctgttcctg gacttggata aggtttgtta gttgagactt
    11581 gctgcacttg attggttgct agaacggttg ctttcagctg atcaaatgca tgttcgaagg
    11641 aatctccagc atcaactata ctagctttca tcgttccgac ttcacggatt ttgcgagtaa
    11701 gggcgcgcgt atcgatacct gatattccag gaattccttt ttgtttcaaa aatgaatcta
    11761 gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg ttcttttact actactcctt
    11821 tgcatgttgg agaaatcgat tcataatcat ctctatttac cccataatta cccaccatag
    11881 gataggtaaa tgtaatgatt tgaccgttaa aactttgatc agtgatagtt tcttgataac
    11941 cagtcatact tgttgtaaaa acgatttctc cgaagacact agcatctgca ccaaaagcct
    12001 ttccttcgaa aactgttcca tcttctaata taagcaaacg ttccatactt ttattctccc
    12061 ttctgccaca caagttttcc gttgacaaaa gtcatttgtg tttcgccttt tactttccag
    12121 ccgataaaag gtgtattctc cccttttgat aaaaagtctt ctttatcgat cgtgcaagtt
    12181 tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa ccgttagcgt tcctgcattc
    12241 aaaccaaaaa tctcagccgg tttaactgcc aaccaattga tcacttgttc caacgtgaat
    12301 cgaccagttt ctacaaaatg agtatagatc agttgaaacg ctgtttcgct tcctacgata
    12361 ccaaatggtg atttcatgaa actttggctt ttttcttcta atccatgggg ggcatggtct
    12421 gtagcgatgc aatcgatcgt accgtcaagc aagccttcga tcaaagcttc tctgtcctcc
    12481 cgtcctctta gcggcgggtt cattttccaa aagccgaaat cttcgggaat atcttcgtcg
    12541 atcaaaataa gatgatgggg tgaaacttct gctgttacgt ggattcctgc tttttttgcg
    12601 tctctgatta cacggacact ttctttagta gaaacatggc aaacatggta atgaacacca
    12661 gttgcttcag ctagcaacag atctctagca atttgagaag actcggttac gcttaagatc
    12721 ccgggaagac caagttcttc tgccttcttg ccagcgtgca taacgccgcc aaataataaa
    12781 gattcatctt ctgtatgggc tactagggct ttattgtttt ttgcagcttc tttcatagcc
    12841 agatacatcg tcccagctgt ttggacacct acaccgtcat ttgtgaaagc aaaagcacct
    12901 tcttcaatca gtgcttcttg atcaactaat ttctcacttc gcagattttc tgtgattggc
    12961 gcatactgca ataccttcac aactgcatct tttctaatga tgtcatatac ttgtctcagc
    13021 ttttctgccg tatcaggtac aggatttaaa ttcggcatcg cgcatacagt agtgaaaccg
    13081 ccacgagcag ctgagcgagt cccagcttcg atcgtctcct tgtatgtaaa tcctggctct
    13141 ctcaaatgaa catggacgtc cactaaacca ggtgtgatca attgtccctt tgcatcaaac
    13201 acttcatcaa attcggcttc tgagaatcct gtaccgattg ctttgattcg cccatcttct
    13261 atccaaattt ctgctggtgt cgtcatattt tttcgtgtat tgatctgacc gtttttaatg
    13321 agtgttttca ttttttcatc ctcctataat tacgcttttc catttagcac tgcttctaag
    13381 attgccattc tgacgaatac tccattgctc atttgctgaa cgatccgcga ctgtaatcct
    13441 tccactaatg aatcagctaa ttctacatca cggttaactg gtgctgggtg cataatgatt
    13501 gcatgttttt gcaacttctt cgcacgctca actgttaacc catgttcctg atggtagcct
    13561 tcttttgaga aactttcttt tccatcatgg cgttcgtgtt gcacccgaag catcatcatg
    13621 acatcaactt gatctagtag ttcatccaat ggcatataat gtccgtacac ttcgaattca
    13681 tcgtcgtacc attctctagg acctgagaag aatacttgtg cgcctagacg ttttaacatc
    13741 tgcatattgg atttagctac gcgagagtga gtgatatcac ctacgatggc aactttcaga
    13801 ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat caagcaagca ttgggttgga
    13861 tgctgtccgc ttccgtctcc tccattgata atcgaacact ggattgtttt actttgtatc
    13921 agttcgtcat aatagttctc atcaccatga cggataaccg ctacgtctac gcctaatgct
    13981 gacatcgtca gtactgtatc gtatagtgtt tccccttttt ggacagagct tgtgcttgct
    14041 tcaaattcaa tcacttctag ccctagtttt ttctccgcta catcgaagct tttatgtgtg
    14101 cgagtactat tttcgaaaaa gaggtttgta gcaaagtact ggcgcttttg cggagtccaa
    14161 ttagcacctt gcttgaattc ctgccctctg cggatcaagc ccataacttc ttgatcagta
    14221 agtgcctcaa cggtcaataa atgctttaag ctgatacgtt ctgatttgat gatcataatg
    14281 tttcctcctt agttttcttc gcttcgtgaa gtttctggta agataagatt taaaatgatg
    14341 ccaagtacag ttgctaatgc cattgccgac aatgtaaatg tgcctacttc aaagacgagt
    14401 ccgccaattc ctactactaa gataactgaa gcaatcaaca gatttttctt tttatcaaaa
    14461 tcgattttgt tctcgattaa aatcttcatt ccacttgcgg caattactcc aaataagata
    14521 aaactgattc ctgagatgac tggtccaggg atacttaaaa tcaaagcgct taattttcct
    14581 acgaatccta aaatgactgc aaatactgca gctccaccga tcacaaacac gctatggaca
    14641 cgtgtgatag ccagaacacc aatgttttca ccgtaacttg ttacaggagg acctccgaca
    14701 aaggctgcca cgatttgtgc taatccatca cctgtcaacg tcttatgaag acccggttct
    14761 tcaaagaaat tacgtttagt tagtttattc aataccataa gatggccgat atgctctgtc
    14821 atcgtcacaa aagcgatggg tgccattgtt gtgattgctc caagatatag ctttggttca
    14881 tattgtacaa agggaatctc aaagttagga acagcaaacc aggcagcgtc tacgattggc
    14941 ttagtatcca cgattccaaa cagcaaggct acgatgtatc cgctaataat acctagtaaa
    15001 atcggaatca atccgagaaa acctcttagc cacatgttga agaaaattgt taatcctaat
    15061 gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc cctgattata catagcattt
    15121 gttgctgcat ttgccgcaag tcccaacccg ataaccatga tgactggacc gacgacgatt
    15181 ggaggcaaga ttttatccag ccatgctgaa ccgatctttt tgatgatcag cgcaacaatc
    15241 aaatagacta atccagttgt gactgctcct tgtgcaatcg ctggataacc atctgttttc
    15301 atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc ctaagtacgc aggtatcttt
    15361 cctttagtag taaataagta caccaatgta cctagtcctg aactgactag cgcaatacct
    15421 ggatcgatac ctactaagat ggggactaac acggttgctc cgaacatcgt gaacaagtgt
    15481 tgtaaactta atcctaccca atgtcctggg gttgggcgat cgtggatatc taacaccacg
    15541 tcatcattat gaaatccttt tttctccgac atgtttctct ctcctctaat cttcctcttt
    15601 caaaatcatg atgcgatctt gtccatcaag ctcttgcatc tctactaaaa tctcttccgc
    15661 ccgagatgtt ggaatgttct tcccaacata atctgcacgg attggcaatt ctcgatgccc
    15721 acgatcgacc aggactgcta atgaaatttt tcttggtctg ccaaaatcca ttactgcgtc
    15781 cattgctgct cgaatcgttc gtcctgtata caaaacatca tcaatcaaaa taacttcttt
    15841 tccttctaag gaaactggga tgtcagaaga atgaagttcc ggttcttctg gattttcttt
    15901 tttgtcatct cggtataaag tgatatccag ttctcctaca ggaatatcga tatcttctag
    15961 ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt cctcgtgttt tgatacctac
    16021 caatacgata tcttggatac tatggtttct ctcgataatc tcataagtaa ttcgagtcaa
    16081 tgctcgcttc atggttacct gatctactac ttcttttgct tgcattttca ttccccctac
    16141 ttttttgcat aaaaaaactc ctgccctaag aggacaggag gaatgtttag actgagcgta
    16201 tactgataca ctgatagcta tactatgtca atgagcaatc tacttatggc gcaccatacg
    16261 tatccttgct cccacccttc ttagcctcac gggactaaac ttaaaggaat tctattaagt
    16321 tttatgcttt cgttgttaat tactataccg ttatctaaat taaaatgcaa tgttttttct
    16381 taatttttct aaagtttttt caaaaacttc tggaagaggt gcttcaaaga tcatcttttc
    16441 gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg agaaactgac cattgccttt
    16501 caaagttttc ttcggtccgt atactggatc tccagctaac ggataaccga tatacttcat
    16561 atgcacacgg atctggtgtg ttcttccggt ttctagtttt aattcaacca gagtaaatcc
    16621 ttcgaaacgt tccaatacag taaaatgtgt gacagccgac ttcccatctt ctcttatcgc
    16681 ctgcattttc cggttttcct tcgaacggcc gattggcgca ttgatggttc ctttttcgtg
    16741 aggaatctct ccatggacta aagcaacata tttgcgcaaa gaggtcttat cttttaattg
    16801 ctgagctaat gattcgtgtg cctggtcatt tttggcaacc attagcagtc cggaagtatc
    16861 tttgtcgatt cggtgaacaa tacctggacg aataacgccg ttgatcgtag aaagatcctt
    16921 gatatggtac atcagcgcat tgaccattgt gccatgcgta tgacctgctg aaggatgaac
    16981 gaccattcct tgaggtttgt ttatcaccgc tacatcttca tcttcgtaaa cgatcgtcaa
    17041 cgggatattt tctgggacca catctaatgt ttctggttcg gggatcaaga cagtaatctc
    17101 gtctccttgt tttactttgt aatttgcttt gactggctgt ccattgacag aaaccgctcc
    17161 atttttcagc cacagctgaa tttgcgaacg actgtaatcg gtgaataaat tagtcaatac
    17221 tttatcgatc ctaccagatt catggtgaat cgtcgtttta agttctgtca tttttttctt
    17281 tgatcctttt catctaaaat aatataaatt aacatacaaa tcactccaca tacaagtgtc
    17341 acatctgcta cgttaaaaat cggaaagctc ataaaatctg tctggaacat atctactaca
    17401 tagccaagac gaatacggtc gataaaattg ccaatcgcac cagctagaat caaactcaat
    17461 ccaatcatga accagcgatt acctttttca cgatttttaa tcatcaatgt gagaatcaca
    17521 acgactgcaa tgagtgtcac tatagtaaag aaccacatct tcccttctag catactccat
    17581 gcggctccag tgtttcgaat atgattcagt gacaaaatac ctggtataag atttttcgtc
    17641 tctccaagcg ctagattatc agtgatccac catttgacca actgatccac tcctactagc
    17701 aaagcgctga atatccaata aataattaac aaaaaatcaa tccttttcta tttgtttata
    17761 aatatcacga ggtcgtacga tcccgagcag attaccatcg ggattcccat ctttagtgat
    17821 aagtacggct tccaaagtcc cttgctcacg aaacatcgct tcaacctcgt aaatatgtgt
    17881 tttagctgga acaaaatgat aatttgtgta ctttccgttt tggatgatca aatccaacgc
    17941 tcgtttattt gttaaatcaa ccggaccttt ttggctttct tttgctagcc aaaacccgat
    18001 attacgtaaa gtaatcaatg ctacaaattt tcctttattg tataacggaa attgtgaata
    18061 ccttttttct gctactatct ttaacagttc aagtaacgga agatcttgtt caaaacctgt
    18121 tacacgtttt gcaaatcgag gcagcacggt ttcaggctga atcagttctt gttcgatcag
    18181 ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg acaaaatcga ctgcgatccg
    18241 atcatggacg atcgcattcc ttaactgagc aagctgtaaa agatcatctt cgtattttct
    18301 gatcattaat tgttttcttt gagctaggcg tctgacaagt tcggtaaatc ccatatttcg
    18361 tgcatttccc atctcgtctc tcatccattt ttcgattcga ttgaaactga ttaaaaaaag
    18421 atcagagttt gccataattt tcctccttga tcaaatatgc ccggtttttt atatagaaaa
    18481 agaacgcaat caacacaata actgcaaaaa aactgaaaaa taaattttgt aggaaaacgg
    18541 cccccaaccc agttgctaga aataaaaacg gatagatttg aagaatcaag taattcattg
    18601 tgatcacata agcaggcttt gcttctttca ctgtataaac ggggcttttt agccaataaa
    18661 aggtgacctg catcgtaaag ggcacttcca gatcaagcgt tatcttttgt tcatgactga
    18721 gaacactgcg tttttcattg ttgattctta gctgaatcga gctacccata ccgtaaaaac
    18781 cggtttttct tataaatgtc gttttcatca taccctccta ttatgcttta gtatagctca
    18841 aaatacaata aagaaaaaga atgatcctag tgtaaacaaa attaattccc tatcgtctaa
    18901 aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta gattataatc aagcttaaaa
    18961 caatcccaac acttctccgt catttgtcac gtccatattc aatgcagcag gttttttagg
    19021 caaaccaggc atcgtcataa catctccagt caaggctact aaaaatcctg cccctaattt
    19081 tgggatgatt tcgcggatcg tgatggtgaa atcttccggt gcacctagca gtgaaggatc
    19141 atcagaaaat gaatactgtg tttttgccat acaaatcggc agtttgtccc atccattctt
    19201 cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa acaacaccat ttcctccgta
    19261 aattttcttg acgattgttt ctgtcttttc ttgtatcgtt tcttctaggc ggtataatgg
    19321 cttgtaatta gcttcttttc tatcaatcaa tcgtactaca gcttttgcta aatcaacgcc
    19381 gccttctgct ccatctgccc atacactcgc tctcttggca agaatcccct gattttcaca
    19441 caagtgctcc aacagtgtga gttcattgtc tgtatctgtt acgaattcat tgattgcgac
    19501 gatcacaggt agctgatatt gttccatatt acgtatatgg cgctttaaat ttgcaaaacc
    19561 tactttcaaa gcatcaagat tctcattttt aagttcatct tttttcattc cgccatgcat
    19621 tttcaatgca cggacagtgg cgacaatcac cacagcatcc ggcgcttttt tcaagttagg
    19681 tactttgata tctaaaaatt tctctccgcc aagatctgca ccaaatcctg cctctgtgac
    19741 aacgtaatct gctaatctca atgctgtctt ggtagctaaa atactattgc agccatgagc
    19801 gatattggca aacggtcccc cgtggacaaa agctggtgtt ccgtaaattg tttgtacaag
    19861 attcggcttg attgcatctt tcagcagtaa tgccagagcg ccttctgctt tcaagtctcc
    19921 agcagtcaca ggctgacggt caaatgtata gccgacaacg atccttgcaa gacgagcctt
    19981 taaatcttca aggtctgccg caagacacaa gatagccatg atttcgctgg caactgtgat
    20041 atcaaaccca tcttctcttg gaacaccttg gattggccca cccaagccaa caattacttt
    20101 acgcagttca cgatcattga gatcgactac ccgtttccaa atcactcggc gggcatcgat
    20161 gttcaattca tttccttgat gaatatgatt atctaaaaga gcagataatg cattattagc
    20221 tgttgtgata gcatgcatgt caccagtaaa atgcaagttg atatcttcca taggcaatac
    20281 ctgtgcatat cctccgcctg cagcaccacc tttgattccc atcacaggac caagtgaggg
    20341 ttctcgcaat gcaataacag attttttatt gattttattg agcgcgtctc ctaaccctac
    20401 agtgatcgtt gactttcctt cgccagctgg tgttggattg atggaagtaa ctaagatcaa
    20461 atgcccctca gcgttatcct ttgatcgatt gattccagaa aaattgattt tcgccttata
    20521 tttcccatac agttccaaat cgtcttcctt aagatctaat ttactagcca cttcaacaat
    20581 cggttctagc tctgtttctt gtgaaatttg cagatcattc ttcatttgat tacccctttt
    20641 cacgaacttt attcaacaaa atatcttctt tgttaagata tgccaattat accttttttt
    20701 attcagaaaa aaaacataaa attacaggtt tttaatttat ttgacgtact tataaatact
    20761 ctttataatg aaggctatga tcagacttta gaaaggtcgt gagtgctttg accacaggaa
    20821 ttgtaaaatg gtttgacaac aaaaaaggat acggctttat cagttatgat gacacagaag
    20881 aaatttttgt acattttact gcgattgaag aagagggatt caaaactttg gaagaaaatc
    20941 aagtggtcga atttgagatc atcgaaggga atcgaggaac acaagctgcc catgtcaaga
    21001 aaggcaacgt tcagactacc gatactagta tgcatgaata gataaacaaa tagagtgaga
    21061 cgctaaacgg gatgaaccgt acgtctcact cttttctttt cctgtaaaaa ttcgtttttt
    21121 taggttctga tttttaaaat tagctttttc tttttttcgt ataaggataa ggaattgatt
    21181 ctgatttggg tccgtttttt tcaaaatgat caaaaaggac tacttgatct ttttttcgca
    21241 tcatgaaaac agatggggct ttctcatctt gcaacatcat ttgcacccca gtattagttg
    21301 gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc tttccaaaaa gcagcataac
    21361 gtataatcac agagtttgat tttaaaataa aagaacgccg caaacctaga aatagaaaaa
    21421 caagaaaaat acctaaaaca aaattgcttt tccaataagg acctgtgttt tctaaagaaa
    21481 gaatcagact aaaaaacaga atgatcaagg tcaatgacca atagatgatc gtatgtgcca
    21541 gttcaggctg ccatcgaata ttttttttca ttctctaacc tctcaccgat tcagtaatga
    21601 tataatctta ccataacatt agtttgaaca aaaaggagaa tctcttttta tgttaaaagt
    21661 atacattgat gcctctacaa aaggcaaccc tggtcctagt ggaggtggaa tcttgattgt
    21721 ctatcaaggt aaacaagaac agctatctat cccgctttct atagggacaa accatcaagc
    21781 agagttcgaa gtctttttaa aaactttaga atttctaaag aaaaacgact tacagaatga
    21841 aacgattttc tgtttttcag acagcaaaac actagtttct acggtcgaca aaaacgcaac
    21901 gaagaacgag ctcttccttc cctaccttca aagaatccaa gaacttctat ccgaattttc
    21961 tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa ggagcagata atcttgcacg
    22021 tcaggctctc cataaacaat taaaagaaaa aaagactctt taaattaaaa actttaagga
    22081 gtctttttat agtttctcat ttaatataaa agataattgt gatcaaacgt aatttccaat
    22141 tggtaatctc cgattttttc ttctatttga ctacgtaaga ttctttctag ttctgtatca
    22201 acaaactgga atttctcagg gaccactaca tcaaattgca gcgtttttcc ttgatgaatt
    22261 actctaaaat catgaatatt aagccctgta cctgtttcaa gaagaatttg ctttaactgc
    22321 cgcaagatat tatagtattt ttcgttatca acaggcattg gatccaagtg acagacaagt
    22381 tccacatcca aagacttttt aaagtctttt tctatcagat caataatctt atgagccaca
    22441 ttcaaattca ttgattcatc tacttcgatg tggactgatg caaagcgttt attgggtcca
    22501 tagttatgca ccaataaatc atgatagcca agtatatcat cgcaagcatt taaatgctct
    22561 tccatttctt caatctctgc tggcgttggg cgacttccta atagttcgta tacaaaattt
    22621 cgaagcatcg tcaatccact ataaaggata tataatgcta aagcaaaacc tatatatcca
    22681 tcaatacgcc atccagttac ccattcgatt gctgctgaaa gcaatacagc cagtgtagta
    22741 aagacatcat tataactatc tgttgctgta gctctcaatg tttcagaatc aatggattgc
    22801 gcaataacct gatacatttt gccttgtgca aatttcaaaa agatggaaat gattaggaca
    22861 aaaaagacga tgggtgttaa agagacattt tctggatgga tgattttttc agcagaagac
    22921 tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa caaataaccc actgatatat
    22981 tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg gctttgcagc aattttaaat
    23041 ccgatcaaag tcaatatgga tgaagcagta tctgatagac tgttcactgc atcagccata
    23101 atggagacac ttcctgaaaa aagcccgatc attagcttag agacaaacaa gatcagattc
    23161 gtaaccaatc ctaagatacc cgctagtact cctttttttg tccgctgttg cggtatttgt
    23221 ttcttttttt cttcttgttt cttagccaag ttttttaccc cttctagttt gcttgttgct
    23281 caggtaaaaa gatggagagg acgattgttt tcctctcctc tcttcctttt tctatctatc
    23341 gtgcgtccta ttactattaa aagatggtca ttattctggt ttcaacggtg ttgctgcaaa
    23401 taacgctttt gctagtaatg gtgttatgat tcccgcacct attgcttctg gtatcccatt
    23461 gattgctgca acccctgcta aggttttaaa taaaagagca gagtctacac cataggcatc
    23521 tgccgttgca cctgtataaa ataatcccat gaatcctaac actaagactg tattgaccaa
    23581 tgaacccagt acccctgcaa aggctgagct gatcgttatt gatttatttt tttgataaat
    23641 ccagtggaaa accaagcctg ctactaagcc aacaagtact ctgggaacta ccgagataat
    23701 cggattcgtg aacactaatg tatcaaatgg cgttgttggc atggcatagg cacgaacaat
    23761 tgtagcgatt ccccaaaaca agccaatcac catgccatct tttgggccta atactactgc
    23821 agcaataatt actgtgatat ggataattgt taaactaatg aatcctaatg gaataaaccc
    23881 cagccaagga accatcgctt gtacgatgat gattgctaac aagatcgcac gtaagactag
    23941 tcgaaaggtc ttatttctac tattcatttt ttcacccctt tttatttata ctaatcgtaa
    24001 aaatttctgt cactttcgtc aactaaagac catccgaatc cgaccatttg attagatgcg
    24061 ccatccaatt gaaaaaagta tgagggcaag acttgtttgt cttcgccccc ataatcatgt
    24121 ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta aacgccctat tgttgcgcaa
    24181 cccattcttt cgcagcttta agtgcatctg gaataccagc aggatttttc ccgccagctt
    24241 gtgccatgtc tggacgtcca ccaccgccac cgcctacttt cggtgcgatt gctttaatca
    24301 agtcgccagc ttttagtcca gcttcatttg attctttcgt cattgccgct agcagactga
    24361 cttttccttc ttgttcgttt gctaaaacaa gaatatctga aatatttttt tgtttccatt
    24421 ggtcagctaa ttgacgcagc tgattcatat ctttcacttt tacttgtgct gcaatgattg
    24481 tttttccatt gatcgtttct ggatctttga atacatcacc agcttgttga ttcgctaact
    24541 tgctagctaa ttgttcattt tccttttgta gctctcttac ttgctgttgc aattgttccg
    24601 cttttgaaac aacttctttc aattgaggtg atttaacaat tgttgctact tgttttagtt
    24661 gtttttcttc ttcttcaagt agttcgtacg cttctttact tgttacagcc tcgattcgtc
    24721 gcacgcctgc accaattcct gattcagaaa cgattttgaa gattccgatg ttttctgtat
    24781 ttgctacatg tgtgcctccg cacagttcga ttgaccagcc gccaatattt actactcgaa
    24841 cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc catgtttttg gctgtatcga
    24901 tatctgtttc aattgtttca actgggatag cttcccagat tttttcgttg acgattcttt
    24961 ccatttcagc taattcatct gaagtgattt gaccaaagtg ggtaaagtcg aatcgtaaat
    25021 gtccaggagc gactaaagaa ccagcttgat ttgcatgctc gccaagtact tctttcaatg
    25081 ccttgtgcag tagatgggtt gctgtatggt ttttgatgat ccgattgcgc atcgattgat
    25141 cgatcaatag atgataggtt tgtccttcac tcattggtgc ttttacttct acggtgtgca
    25201 taaattgacc attaggcgct ttttgaacat ctgtaacaac tgctacgatt tcgcctactt
    25261 cattcatgat ccatccttga tccgctactt gcccacccat ttctgcatag aaaggtgttt
    25321 gatcaaagat catttgagct tcaccttctg cgacttcatt caaaagagac tcacctttaa
    25381 cgatgaccaa taattgacta tctgattctt gttctgtgta tccaatgaat ttactttcta
    25441 ctttgatatc tgtcaataaa gcagattgta cccccattga tttttcagta ctgcgggcag
    25501 agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa accagcatga tcaactttca
    25561 gtccttcatc ttctgccacc tcttccgtta attctaccgg gaagccatat gtgtcgtata
    25621 gtttgaagat gtcttttcct gctagagtcg tcccgttttc gttttttact tcttggatca
    25681 cttgatttaa gatttccaaa ccttcattga ttgtttcatt gaaacgttct tcttctgtac
    25741 ggataacttt ttcaataaat tctttttgtt gaagtacttc aggataataa cttaccatga
    25801 tttccccaac aacagggact aaacgatata agaatgcttg ttcaatacct aattttttcc
    25861 catgcatgac agcacgacga agcaaacggc gtaagacata ccctctccct tcattcgatg
    25921 gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat atgatctgcg attactttgt
    25981 aagaaatatc tgttactggg ttttcaccat attttacttg tttgcttagc ttttctacgg
    26041 cgtggataat aggcataaac aagtctgtct caaagttcgt tgacgcatct tgaacgattg
    26101 agaccatgcg ttccaatccc atccccgtat caatgttttt atgaggaagt ggttcaaatg
    26161 tgttatcagg cttatggttg aattctgaaa atactaagtt ccagatttct aaataacgtt
    26221 cattttctcc tcctggatag ttttcaggat cgtcttctgc taaatcgtta taagcttctc
    26281 cacggtcata gaaaatttcc gtatccggac cacatgggcc tgcaccgata tcccagaagt
    26341 tgtcttctac atcaataatg tgatctagtg gcaggccaac ttcttcatgc cagatgcgtt
    26401 ttgcttctgt atcttttgga taaactgtta cataaagttt ttctggatcg aatgccatcc
    26461 attttggatc agtcaaaaat tcccatgccc agtggatggc ttcatttttg aaatagtcac
    26521 caatcgaaaa attacccagc atctcaaaca tcgtatggtg ccgtgctgtt ttaccgacat
    26581 tttcgatatc atttgttcga atagattttt gggcatttgt gattcttgga ttttctggca
    26641 caactgaacc atcaaaatat tttttcaatg ttgcaacacc agagttgatc cacaataagg
    26701 ttggatcgtt cacggggatc aaagacgcac taggttcaat cgtatgtcct ttagattgga
    26761 agaaatcaag aaacatttgt cgaacttgtg cactacttaa ttgtttcatg tcattacctg
    26821 ctttctataa attaaataca aaaaaacacc atcacagcca aggacgaaaa ctcgcggtac
    26881 caccttgatt gcaatgatgt gtaatcatta cctcttaaac tcgataacgc tgagctgcgt
    26941 cggtatttct accaaaatag aaaaagggag caatttgatt agtgactatt cttttttcag
    27001 ccaagaaaga attttctgtg agtcttaaaa aacaaatcat atccttttcg tacttaatta
    27061 gtatacgcat tctattgatc actgtcaata tctcaatttt tattttcatt tccttttggt
    27121 catactccac gatttttggt acgataagaa taaaggaatc aatacatctc tgctattcat
    27181 gacaaggagg aatatgatga aaaaaatagt tggagcggat gaatggatac gcgcagctag
    27241 ctgctttttg agatatcaag tttttgtatt agaacaaggg attcctgcag aactagaatt
    27301 cgacggaaaa gatatcccgc aacaaaccta ttatctattt cttgatcaaa aaagaccagt
    27361 agctactttg cgttatgaaa agaaaacacc tacttgcttc aagccggaac gattttgcgt
    27421 agcaaaagaa taccgaggaa aagggttagg cggtcagttg cttgaagctg ctgaaaaacg
    27481 ggcggtttca gaaggttgtc agactagtta tgtagtagct gaaatgacgg ctgtagattt
    27541 ttatgaaaaa caagggtatc atacttgcac agaacctttt attgaagatg aagtactttg
    27601 tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt gtgacacgcc ttttgtcaca
    27661 acctcttgtt ttttatttat gtgaattttt cttattttta cgagcagtac ggctttgctg
    27721 tctgcgttcg attttgcgtt cttttttatt tttttctgca attgcccaat cgattttttt
    27781 cttataacca ggcttgatct ttttcttttt cttcttcaca agaccaatca gcgtgggatc
    27841 taatacatct tttgattttt ctcgtttggc acgtctatta cgatcgtatg ttttgacgat
    27901 ttccccgttt ttgatttctt ttggttcaaa tgagacgccc aatttttcta tcgcatcaat
    27961 ggcttgctca tcatttggcg aatataacgt aatggccgtt ccttttaagc cattacgtcc
    28021 agtacgtccg acacgatgga taaagaagtc tagatcttct ggaatttctg cattaataac
    28081 atgagagaca ccttcgatat cgatccctct agcagctaaa tcagtcgcta caacatattg
    28141 atactccaga ttctgtactt gtcgcatcac tcgttttctc tctcttgggg tgatgtctcc
    28201 atgaatcttt gctactttca agccttggtt tttcaagtaa tcggcaattt catccacatg
    28261 ctgttttgta ttggcaaata cgattgctaa ataaggatga ccaattgtca gcagttgata
    28321 aatcatttga ttctcatctt taccttttgt tgaaatcagc caattttcaa tgtcttcaga
    28381 aataactgct tttggtttga tttcttcaat gattggattt tccatgtatt ttcgtaagaa
    28441 tggacgaagt ttttctggaa tagtggcaga aaaaactaaa aactgcagct tttctggtaa
    28501 tcgacttgca atttggtcta cttcatttaa aaaacccata tccaatgtca tatccgcttc
    28561 atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct tgttcattca tcatgtctaa
    28621 tattctacca ggcgtgccta ctacaacctg tggctgttgg tgatgcagac gatttaattg
    28681 acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg atctccggtg tagaaaattt
    28741 agcgatttgt gtagctgctt catagatctg tgcagctagt tccctacttg gagctgtaat
    28801 aacaacttgt acttcctctt tttctgcatt gattttttca aacaaaggca acaaaaacgt
    28861 atgtgttttt cctgtaccag tttgagattg cccaatgata ttttttcctt ttgcaattaa
    28921 aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag ccctttgcgg ttaacgcttc
    28981 attgataaac ggtttgaagc ggaatatatc aaaattattc attcgtgcac ctcttctctt
    29041 taaattatca cgactttggc attataacac agacaaagcg tttttgctca gctaatttcg
    29101 agaatcatca tcctttgttt ggccaatcgt gctaacagta tatgtgtgac tagctttttc
    29161 ataaaacaga atccgttttt ttctcaaaaa tgatagcaag atatttaaaa taaaaatacc
    29221 ttgtagtaac aagacaccga gagaaatgaa tactgcactc atctgcaaca ccgtattcgt
    29281 tgtcgaattc atgatttgct gaagcaactg ccccaaccag cttaaaccat aaaaaagaaa
    29341 gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa attcgttcgc ggtcttcttc
    29401 caccaaacgg atacgcacaa tttttttgcc aagtgtccat ccattggtta taaacggtac
    29461 aatcataaag tataccccta cttctaataa aagccaccag atatgagaag taactgcccc
    29521 tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa ttaataagag ataaaaatgt
    29581 ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag acagtctttc ctttttcata
    29641 agaaagttca tccattcgtc ttcttgtagg aagcataaat gtaaataacg gcgtaacgac
    29701 aaaaccaatg attccaccca atgtatttgt cactaaatca ttcacatcaa ataagcggta
    29761 tggtcttggg tagataaaat aaagtccact tagctgcgtc aattcaaaaa atagagaaag
    29821 gcagaaacta gcaatcatcg ttttcataaa agaaagccgg aaataatatc ttaaataaat
    29881 gccaaacggt atgagtaata aaacattaaa agctggttcc aaaaatacac tctgcttcat
    29941 tgccggcagg taggtactag gttcagacaa gaccaatacc gtgttgtctc tgaaatgttg
    30001 ccatgaagca aataaatgca gctccatctt tggccctgtc atttgggcaa ccgtctcctt
    30061 tgccggaagt ggtaagatca ccaaaaaata cgcacacaat aaataaaaaa cgaaagaata
    30121 caaaataact gcccttgtta ctagaaatgt tccatacttt cgatattcat aaataaaaaa
    30181 aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc agcgcaaaac gaatcggttc
    30241 gatataactt gccatatgac tcctccttga ttctttgtat ctctcttatc ttagcagtcc
    30301 tttgctgttt caagcaagta acgaggctat tatttctcat ttctttcaaa gtaaagtaag
    30361 ataaacacaa agggggtgtc gagatgctga atatgtactt tgtttttggc gtacctattt
    30421 ttttactgtt cctttatgct acgatcgctt atgtaagaaa aagaactaca attcattatt
    30481 taggatttat tctcctgatt atttcgggtt tcatgctagt cttcaatctt cagacttggc
    30541 agcaagcact tcttgaaatg gataaaatga ctccacatgc tttgtctaaa gtgcttggct
    30601 accctgtcta tctgatttgg cttcccattt tcatttctgg ttgcttggtt ttattaaata
    30661 tttatcgcgg cgtacgccga atagttcagt tacgcaaatc aaagtaaaag acagaaatcg
    30721 gttgagcccg atttctgtct tttactttgc tatttcatcg tagacaaaac gacatttacg
    30781 tgatcaaaat taacgagtac ttttgacatt tcttccacgt actcctcatt taaataaaat
    30841 attttgtttg gcagagaagt gcagttctaa taaaattcga atgttttagc tatctacgtt
    30901 ttaaatagag ataaatcggg ataccagcaa gtgtcaatac tatccccatc gaagctaaaa
    30961 aggtctgagt gataagcgtg cttgatacga taaaagtccc tccaatgatt gctactagtg
    31021 gaataaacgg atacattgga actttgtatg gtcgaaacaa atcaggttct tttttacgaa
    31081 gaagaatgac accaacaaag accatcgtat aaaaaatcca aatgacaaag atcagcatat
    31141 ccgttagtgt atcaaaacca ccgatcatca tcatgccaat agcgatgact agctctaaga
    31201 tacctgcggc tacaggaact ttagttttat catgaagttt agcaaagagc ttgctaaaag
    31261 gcaggttgtt ttcttttgcc ataacataag ggagacgcat tccagtaagg gtatagccat
    31321 tgatggttcc ataaacagaa atcaagatac caactgtgac cagtcttcca ccgaagccgc
    31381 caaaaatcat ttttgcaacg tcacttgccg cattgctgtt tcctgcgact ccgtcgatag
    31441 atgcggttct taagaacacc gcatttacta ataaataaac gatcatgatt ccgatgattc
    31501 ctaaagaaat tgcttttggc aaatctttcg ccggcttttt caattctccc gaaatatttc
    31561 ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag caagcccgct cccaaggctg
    31621 agaaaaatga aagattttct cctgcttgga tcggaaacag ttggaagtct actccttctt
    31681 gtcggaacaa accgaaaatg acgatcacaa acaaaggaat aagcttgcag acaagcgtaa
    31741 tcgactgaaa agcccctcca gctttagaac ctaaaaaatt gatcaacagg atcgatacag
    31801 cagctgtaac agctacggga acaatcattg actgggaaag accaaataga ttgacgaact
    31861 gtgtaccgaa aatgatcgac aaggctgcta catttgctgg aaaataaatc acgacttgcg
    31921 cccagcccaa taaaaaagct gctgtatttc cgtaaatccg ttcgatatat ttaatcatcc
    31981 ctcctgtttc tggaatagca gctgccagtt cagctcctgt tagtcctgca cagacagaaa
    32041 tcataccacc gagaaaccaa gaaaacatat gcaaactggc ggatcctgta acttctgcta
    32101 cgcttgcggc tttaaaaaat acccctgcac cgatcactgt gcccataaca gtcgataaag
    32161 cagtaaaaaa tcccatcgta cgttttaatt cattttctat ttttacttct tttactggcg
    32221 tggcttgata ttcttccatc ccttgtcccc tttcatacca tagccaatcc caaaagtata
    32281 aaaaactttt tggatgtggt tcttgtttta taccgtctct accaattttt ctttcagttt
    32341 ttccaacata tcctttgtca tcacatctaa gtcgtatttc ggcgaaaagc cccattcctc
    32401 ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct gctattgact gacgtacagg
    32461 atccacatca taatccattt caaaatctgg tatgattttt tgaatagatg ctttgatctc
    32521 ttccgggtca aaagacatcg ccgatatatt gaaggcattt cgatgaacca atcgatcagg
    32581 atctgcattc atgagtttta taatggcatc gatcgcatct ggcatataca tcatatccat
    32641 tgatgtacct tgatcaatga aactagtgta ctttccttct ttgattgctg aataataaat
    32701 atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt gcataagaaa tcaatccagg
    32761 gaaacggaca ccacgagtat ctacgccata tttcacataa taataatcac atagtagttc
    32821 tccagctacc ttcgttaccc catacatggt tgtaggacgc tgaagagtat cttgtggggt
    32881 atgatctttt ggagtatttg gaccaaacgc gccgattgaa cttggcgtaa aaaatttcag
    32941 ctggaattct ctagcgactt ccaatgcatt gaccagccca gtcacgttca aattccaggc
    33001 taattgtggt tttgcttctg caacagctga caaaagagca gccaaatgaa tcaatgtgtc
    33061 cacttgatat tgttctgcta tgctgcgcat tttttcgtaa tccattacat ctaggatctc
    33121 aaagatatcg tttttctcaa tgacttcagg catgcgtata tctgtcgcaa tgacattttc
    33181 actgcctagt tcttgacgta gccgtgtgat caactctgaa ccaatttgtc ctaaacatcc
    33241 tgtcaccaat acttttttca tgatgatcct ccctgatctt ttttactact atcttcagat
    33301 gattaacaat atatgcgtga acatgttctc taattttaaa tagacgaaaa aactaaattt
    33361 agttaaataa tttccatttc ttttcctatt ttttgataaa ctgctactgc ttcatcaagc
    33421 atttccctcg tatgttccgc agtcggcata tttctgatcc tgcctgtccc aagaggcaca
    33481 gttggataaa cgattggttt agcataaatc ccatgttcga tcagcttctt agagaaagtc
    33541 tgagtcactt tttcgtctcc caaaatgact ggagtaatag gtgtttcaga cataccgatg
    33601 ttatagccta cttttttcaa ttcttcttta aaataattag cattttccca aactttttcg
    33661 acaagttccg gatgttcttg catcaaagta atcgaagcca atgcagctgc agctgcacct
    33721 ggagttagcg aagtagaaaa taggaatggc cgacttctag ctttcaacca gtcgattagt
    33781 gttttcgatc cagctacata acccccaaca acacctatag ctttcgataa cgtgcccatt
    33841 tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg ttcctgcacc ttttccagtt
    33901 acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc catatttttc aacaatcgga
    33961 ataatttccg gcaatcgtgc aatgtctcca tccatggaga aaacaccatc tgtgatatac
    34021 atgatttttt tatatttctt actttctatc gcttcctttg cttttttctc aaggtctttc
    34081 atatcttgat gtttaattcg gatgattttt gctccggata agcggcaacc gtcaataata
    34141 gatgcatggt ttaattcatc cgataagata gcatcttctt ttgtcattac tgcagagata
    34201 gcgcccatat tgcagttgaa tccagattga aaggcgattg ctgcttctgt ccctttgaat
    34261 tctgcgattt tttcttctaa ttgctgatgg attttcagcg aaccattgat cgttcggact
    34321 gctcctgctc ctacaccata agtttcggtt gcttcaatac atgctttttt caattcttcg
    34381 cgattggcaa atccaagata attgttggat gctagattga tcatcttttt tccgtctacg
    34441 atgatacaag caccattttc actttctagt atgtcgatcg tattgtataa ccccttttct
    34501 ttcagttgat ccaattcttt ctctaaatac attgttagtt ctttggaaga cattgtctcc
    34561 acctccatat tcatctgtct tgcagaacat accaaacata tatcgtgtca ttcatctaca
    34621 aagcaagtat gctatatttt tgataacgct tacactgatt atgagaatta tttaaccaca
    34681 ttttttattt acagtatctt tttactttca aatgacttat atagtatgaa caattacctt
    34741 ttagataatg atctatttta ctctacctgg gcagataaat acaacacaac cattgataga
    34801 agccctttcg ttaaaatcaa ttgtattctg aatatttaat ttatttattg catagaccaa
    34861 taaaaataca taaaatttcc atcttcgttt atcagcaatt tttaatttta ctctctgaat
    34921 atttcttaaa agttttactt gttgaattcg aaaaaaggac tgtgacaaaa gtcttgtcac
    34981 agtcctattc ttccgaata
//
LOCUS       DAZKOB010000017        34521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000017 DAZKOB010000000
VERSION     DAZKOB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.017"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..813)
                     /locus_tag="ACG8M6_000866"
     CDS             complement(<1..813)
                     /locus_tag="ACG8M6_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0456555.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTR"
     gene            complement(1037..2977)
                     /gene="thrS"
                     /locus_tag="ACG8M6_000867"
     CDS             complement(1037..2977)
                     /gene="thrS"
                     /locus_tag="ACG8M6_000867"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0456556.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3314..3493)
                     /locus_tag="ACG8M6_000868"
     CDS             complement(3314..3493)
                     /locus_tag="ACG8M6_000868"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0456557.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3540..5363)
                     /locus_tag="ACG8M6_000869"
     CDS             complement(3540..5363)
                     /locus_tag="ACG8M6_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0456558.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5641..7920
                     /gene="helD"
                     /locus_tag="ACG8M6_000870"
     CDS             5641..7920
                     /gene="helD"
                     /locus_tag="ACG8M6_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0456559.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8013..8660)
                     /locus_tag="ACG8M6_000871"
     CDS             complement(8013..8660)
                     /locus_tag="ACG8M6_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456560.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8839..9558)
                     /locus_tag="ACG8M6_000872"
     CDS             complement(8839..9558)
                     /locus_tag="ACG8M6_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0456561.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9689..10840)
                     /locus_tag="ACG8M6_000873"
     CDS             complement(9689..10840)
                     /locus_tag="ACG8M6_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0456562.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11057..11797)
                     /locus_tag="ACG8M6_000874"
     CDS             complement(11057..11797)
                     /locus_tag="ACG8M6_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0456563.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11801..12124)
                     /gene="rsfS"
                     /locus_tag="ACG8M6_000875"
     CDS             complement(11801..12124)
                     /gene="rsfS"
                     /locus_tag="ACG8M6_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0456564.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12181..12774)
                     /gene="yqeK"
                     /locus_tag="ACG8M6_000876"
     CDS             complement(12181..12774)
                     /gene="yqeK"
                     /locus_tag="ACG8M6_000876"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0456565.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYSASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12758..13408)
                     /locus_tag="ACG8M6_000877"
     CDS             complement(12758..13408)
                     /locus_tag="ACG8M6_000877"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0456566.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13418..13729)
                     /gene="yhbY"
                     /locus_tag="ACG8M6_000878"
     CDS             complement(13418..13729)
                     /gene="yhbY"
                     /locus_tag="ACG8M6_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0456567.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13745..14854)
                     /gene="yqeH"
                     /locus_tag="ACG8M6_000879"
     CDS             complement(13745..14854)
                     /gene="yqeH"
                     /locus_tag="ACG8M6_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0456568.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14851..15378)
                     /locus_tag="ACG8M6_000880"
     CDS             complement(14851..15378)
                     /locus_tag="ACG8M6_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0456569.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15558..16343)
                     /locus_tag="ACG8M6_000881"
     CDS             complement(15558..16343)
                     /locus_tag="ACG8M6_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0456570.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16336..17205)
                     /gene="accD"
                     /locus_tag="ACG8M6_000882"
     CDS             complement(16336..17205)
                     /gene="accD"
                     /locus_tag="ACG8M6_000882"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0456571.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17206..18585)
                     /locus_tag="ACG8M6_000883"
     CDS             complement(17206..18585)
                     /locus_tag="ACG8M6_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0456572.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18593..19012)
                     /gene="fabZ"
                     /locus_tag="ACG8M6_000884"
     CDS             complement(18593..19012)
                     /gene="fabZ"
                     /locus_tag="ACG8M6_000884"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0456573.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19037..19513)
                     /gene="accB"
                     /locus_tag="ACG8M6_000885"
     CDS             complement(19037..19513)
                     /gene="accB"
                     /locus_tag="ACG8M6_000885"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0456574.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19516..20751)
                     /gene="fabF"
                     /locus_tag="ACG8M6_000886"
     CDS             complement(19516..20751)
                     /gene="fabF"
                     /locus_tag="ACG8M6_000886"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0456575.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20770..21507)
                     /gene="fabG"
                     /locus_tag="ACG8M6_000887"
     CDS             complement(20770..21507)
                     /gene="fabG"
                     /locus_tag="ACG8M6_000887"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0456576.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21519..22436)
                     /gene="fabD"
                     /locus_tag="ACG8M6_000888"
     CDS             complement(21519..22436)
                     /gene="fabD"
                     /locus_tag="ACG8M6_000888"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0456577.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22543..22767)
                     /locus_tag="ACG8M6_000889"
     CDS             complement(22543..22767)
                     /locus_tag="ACG8M6_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0456578.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22820..23782)
                     /locus_tag="ACG8M6_000890"
     CDS             complement(22820..23782)
                     /locus_tag="ACG8M6_000890"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0456579.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23792..24232)
                     /locus_tag="ACG8M6_000891"
     CDS             complement(23792..24232)
                     /locus_tag="ACG8M6_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0456580.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24538..25503
                     /locus_tag="ACG8M6_000892"
     CDS             24538..25503
                     /locus_tag="ACG8M6_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0456581.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25560..25790)
                     /locus_tag="ACG8M6_000893"
     CDS             complement(25560..25790)
                     /locus_tag="ACG8M6_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0456582.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25876..26763)
                     /locus_tag="ACG8M6_000894"
     CDS             complement(25876..26763)
                     /locus_tag="ACG8M6_000894"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0456583.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26852..28075)
                     /locus_tag="ACG8M6_000895"
     CDS             complement(26852..28075)
                     /locus_tag="ACG8M6_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0456584.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28092..29138)
                     /locus_tag="ACG8M6_000896"
     CDS             complement(28092..29138)
                     /locus_tag="ACG8M6_000896"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0456585.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29302..31029)
                     /gene="ptsP"
                     /locus_tag="ACG8M6_000897"
     CDS             complement(29302..31029)
                     /gene="ptsP"
                     /locus_tag="ACG8M6_000897"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0456586.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31029..31295)
                     /locus_tag="ACG8M6_000898"
     CDS             complement(31029..31295)
                     /locus_tag="ACG8M6_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0456587.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31774..34008
                     /locus_tag="ACG8M6_000899"
     CDS             31774..34008
                     /locus_tag="ACG8M6_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0456588.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34156..34476
                     /locus_tag="ACG8M6_000900"
     CDS             34156..34476
                     /locus_tag="ACG8M6_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0456589.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9744 a         7475 c         6031 g        11271 t
ORIGIN      
        1 tcgtgttttt ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat
       61 gatatcgatc gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat
      121 ttcttttggt ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc
      181 aaattgcgtc aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac
      241 tacatctgca cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct
      301 gacaagttgc gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg
      361 agaccatttt tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa
      421 aacagtctct gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat
      481 atctactgtt tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta
      541 aaaggactca tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt
      601 tgaaatcgtg attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac
      661 aactgtcggc gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac
      721 cttctcaata ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc
      781 catcatactt tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt
      841 ctatgtaagt ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa
      901 aaacaatccg cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct
      961 gtgtctttga ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt
     1021 acaggaaatt tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat
     1081 caataaagat attcaaatct tctacagctg tttctttact tccataacga cgtacgttca
     1141 ctgttgcatc ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt
     1201 gagaggcacg tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc
     1261 ccttcatttg caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa
     1321 cagggatgat tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt
     1381 ctgttaaata agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg
     1441 gacgatgcgt attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata
     1501 agaagtctaa ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa
     1561 catctagttt tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta
     1621 attcatccat agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa
     1681 aatatttatc tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat
     1741 tgaagtcagc atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt
     1801 gatcaggacg aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac
     1861 ctgacaaagc acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg
     1921 gtaattcacg ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca
     1981 ttgggcgaag aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt
     2041 gataatgatc ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat
     2101 atacatgttg gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc
     2161 ggattgttgc cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca
     2221 tgaacaagtc aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga
     2281 gacggatata ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca
     2341 tcatttgatt gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata
     2401 cttgtatacg gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt
     2461 gttgataagc agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg
     2521 gatcacttgc aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca
     2581 gattttcttt gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta
     2641 cttggttttg tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc
     2701 caaagtggat atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat
     2761 gacggactaa gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt
     2821 cctcgattgg acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt
     2881 ttgctaagct ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt
     2941 ctttgactgc accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt
     3001 ttgattgatc agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga
     3061 tactaggacg aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc
     3121 gttgtaacgg tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt
     3181 tttctcctgg aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag
     3241 ttggctttct catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag
     3301 tgggaaacta aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct
     3361 cattgatgat gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg
     3421 tgatatcttt catcatattt tctaattgtt ccggactgcg accttctact aattcgacat
     3481 gtacgaatgg cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat
     3541 catactatgt ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc
     3601 aaggcactca aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg
     3661 gggattagtc ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt
     3721 acagactgtt tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc
     3781 aagagatcat ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga
     3841 gcccccattg caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct
     3901 cctaccatcg caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct
     3961 tgaggcaagc tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca
     4021 atggcagatc catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc
     4081 ttgattgtct ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa
     4141 gatcgatcaa ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca
     4201 aaagaggcac gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg
     4261 gtcacttctt tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac
     4321 gaacgagcta agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct
     4381 tcttttttta ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc
     4441 gttcctgttt tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca
     4501 gtttttacaa cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc
     4561 gcagctagaa tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg
     4621 tcttttgaga accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact
     4681 gcataacgat cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt
     4741 ttaataatca tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat
     4801 ccgtctccat ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg
     4861 gactcacctg tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct
     4921 gcgggtacaa gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt
     4981 actatatcct gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac
     5041 aaagactgca gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc
     5101 gtaagcatga tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg
     5161 attgcggtaa tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt
     5221 tccaaaaaca tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg
     5281 gccggctggt gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt
     5341 aaaaattttt tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta
     5401 gtaataaatt tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa
     5461 aacaataggc cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc
     5521 tacaaacaaa aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt
     5581 tttcgaaaaa ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg
     5641 atgaatgaga aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc
     5701 gaacaaaata tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca
     5761 aaagacacag ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct
     5821 gttgaatatc gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag
     5881 caaaaacgcc tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac
     5941 tttaaagaag accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg
     6001 gcagaagaaa caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt
     6061 gagattggtc ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg
     6121 aaacgacaat ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta
     6181 atcaatgatg actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac
     6241 atcgtctcta cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta
     6301 atgctgattg aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct
     6361 tttctgttgt atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc
     6421 aaccatttgt tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg
     6481 cctacacaga cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat
     6541 gaacagcaac aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa
     6601 agcggattga ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt
     6661 cccttgttcc gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc
     6721 caatggtata aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa
     6781 acgaagctcc tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc
     6841 aaagatctgg cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat
     6901 acagaacaaa aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga
     6961 ttccgtcgaa tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat
     7021 ctccactttc tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc
     7081 aggtggcagg aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa
     7141 gaggatgctg tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac
     7201 aaagctcgct atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg
     7261 ctgcaagcct tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc
     7321 tttggaaatg aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact
     7381 cgtttccaat taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat
     7441 tttattgtga aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc
     7501 atccaaggaa caacagatga tatcaaattg aattggttag cagataccat tcgaacgact
     7561 cagggaaaag cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa
     7621 gctctttatg ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag
     7681 agtgacttta tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag
     7741 tttgaccgag ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa
     7801 ttgatccttt acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat
     7861 aatgaaagtc ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa
     7921 gtaaaaagac aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt
     7981 gcagaaaaag taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt
     8041 tcaggatatt ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga
     8101 tattctgggt caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa
     8161 attcgattcc attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca
     8221 caatccaaaa tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt
     8281 ttcgtgtcta tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc
     8341 cactctaata gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct
     8401 gaatcagaac gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt
     8461 gcgcggtcca ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct
     8521 aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt
     8581 acgttcacag ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct
     8641 aaagtgattt ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc
     8701 acatgatact tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac
     8761 aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt
     8821 ttcgttaaaa ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc
     8881 ttctagatct tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag
     8941 tgttacctcg ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt
     9001 ttcttttaat gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg
     9061 tccttcttct tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat
     9121 ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac
     9181 cgctccggaa acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc
     9241 tgttcgattc acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa
     9301 tccttcatag cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc
     9361 tcggtcaata atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag
     9421 tttttggttt gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc
     9481 aaattttgca taaatccggc tattattagc atctttagca gttttctttg ctacgatatt
     9541 tgcccattta cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa
     9601 aacgtcctgt tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc
     9661 ttcattcttc tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct
     9721 gttccggaat attgcctccc aattgataga tttgatcact tctaactaac agttccgctt
     9781 cttgtgcctc tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga
     9841 atcttcttcc agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag
     9901 gaatctcttg ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc
     9961 atgtatatct ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag
    10021 ctgctgtttt catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca
    10081 gcaaagtaga agataacaac cgataacgca aatacggcca ataagattcc caagtctgca
    10141 gcgaacaagt tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc
    10201 cttcttctaa cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt
    10261 tttcatgata gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact
    10321 tggcattttc ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg
    10381 taatatcggg atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt
    10441 gatcagacag ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc
    10501 cgaaactgct gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt
    10561 ccagtgtttg caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc
    10621 attcgacggg aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat
    10681 gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa
    10741 tgacgacctc tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt
    10801 gaaaaggatt gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt
    10861 atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa
    10921 ctatataaac tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc
    10981 aatgcctata tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact
    11041 cgagcaggca aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg
    11101 atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca
    11161 gcattcttaa ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa
    11221 taaatcgacc agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt
    11281 gtttctcgct tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat
    11341 agctatattc tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc
    11401 ggccattttc ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat
    11461 ttttcatata gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg
    11521 ataaatcgag catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct
    11581 ggctggcaat catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt
    11641 ttgcaaaggc aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt
    11701 cttcttgtaa atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca
    11761 cttcatcgta aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa
    11821 gatcgactag cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg
    11881 catggaaaac atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt
    11941 ttccttcaat acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg
    12001 taattgcgtt gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca
    12061 aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt
    12121 caatcgcaat ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc
    12181 ttattttttt gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc
    12241 ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc
    12301 agcccatgca atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata
    12361 gtctgctaca taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac
    12421 ccggattgca tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc
    12481 gccaacaaga ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc
    12541 tcttacgatt accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa
    12601 tgcagcaata ctagcttttt caggagaagc actgtatttt ttggcaagag ccacggctgt
    12661 ttcttctact ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg
    12721 catcaggttt tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc
    12781 cttcattttg aatataatca attactttat ctggaacaag gtagcggata gaacagcctt
    12841 ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa
    12901 ttacaggata tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca
    12961 tagaagtcag ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga
    13021 taataaagta ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg
    13081 tgtaactttt cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt
    13141 ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa
    13201 catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag
    13261 cttgttcagc catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc
    13321 ctacttgctt tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa
    13381 caaatgtctt agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg
    13441 atagcctttg atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga
    13501 tttggacgat ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct
    13561 catctgtatt ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa
    13621 tttgaacgat cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat
    13681 gatgcgcttt gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc
    13741 ttccttaaat caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa
    13801 ttactactgg cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt
    13861 ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag
    13921 gaggctgtaa taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg
    13981 ttcgatgaat atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga
    14041 taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa
    14101 ctttaggttt gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt
    14161 gcgccatttg atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta
    14221 aaggaatctc gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt
    14281 gtactcccgc tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta
    14341 ctccgacgat atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg
    14401 tcatctcatg agatttctta gcactagtta ataatacatc tactggacga attcctgctt
    14461 cgtaagcacg ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata
    14521 tatccacttt gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg
    14581 ggattagtga accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt
    14641 gccctaattc attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct
    14701 cattataatg gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt
    14761 tttccaaagc cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg
    14821 caccacagcc gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc
    14881 agggtgtttt gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt
    14941 cttccaacta tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt
    15001 ggctccacgt atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa
    15061 atccaattgt ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac
    15121 atacaaaaga tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac
    15181 aacaacaaca gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt
    15241 tccatctgga ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat
    15301 gcctagattc tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg
    15361 tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta
    15421 ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt
    15481 ctttcagaaa aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat
    15541 ttagaaacaa aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta
    15601 tgctgatcta atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt
    15661 gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg
    15721 attcctaact cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg
    15781 cgactaccgt ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc
    15841 gtttcttcca gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca
    15901 ccttcaccaa tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt
    15961 ctggcaatag cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct
    16021 gtattgatca aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa
    16081 gcttttcgat agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga
    16141 tcccgtcctt tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca
    16201 ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg
    16261 aagatctctt caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg
    16321 acatcatgtg ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag
    16381 cgtatggatt ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga
    16441 taataagaat tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat
    16501 gacgcgtctt ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc
    16561 cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa
    16621 aagaccttca ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga
    16681 aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact
    16741 ttcctttgtt gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact
    16801 tcccatgata aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc
    16861 tttaccagtg aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc
    16921 cgtatattcg ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa
    16981 acttttttca tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc
    17041 gcaatgaggg catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt
    17101 tggacacttt gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct
    17161 attaggattg atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat
    17221 ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc
    17281 acctgctatg actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat
    17341 tccttctgtg ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt
    17401 atttccatgg acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc
    17461 cgggtaaact gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg
    17521 gatcttccct ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc
    17581 gattgcgtgt ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc
    17641 tatctccacc tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc
    17701 cacttggatt cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa
    17761 ctcgatcgtt ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc
    17821 tagtgcttct ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg
    17881 attgttgcgt tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc
    17941 acctaagatc tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc
    18001 atcatttcca aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc
    18061 ttcttttgat tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat
    18121 cactggataa ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc
    18181 agtacttcct ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt
    18241 atttcccatt gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc
    18301 acacatggag gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc
    18361 ggcttttgtc acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc
    18421 ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc
    18481 tgcttctgaa tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg
    18541 tacagcgatt tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa
    18601 tcataaaggt taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc
    18661 ctattccggc tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg
    18721 gtgtaacttt cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct
    18781 tgaattgttc aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca
    18841 atacccctgg cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg
    18901 ttacattttt cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca
    18961 aaagcattgg ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc
    19021 ctctttcctg ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa
    19081 ctgactcgtt ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat
    19141 tcatcagttt catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta
    19201 cttttttgaa agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg
    19261 aaacaacctc gattccatca aatactgccg attctttttc tttcattatt tctggagcag
    19321 ttgttgcttg ttcaggtatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt
    19381 gaaccatatt catgcctcga cttgttttat ttttattcat atacaattca aattgacctt
    19441 cacgcaaatc gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt
    19501 cattgatatc caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc
    19561 aaaccctaat gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac
    19621 aactgtcaag tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg
    19681 atgttctaat gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg
    19741 tccagtcatc gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat
    19801 ttggattgct tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat
    19861 atatccgact tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga
    19921 tgcaccagaa ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta
    19981 tccaacgatt tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag
    20041 aactagaacg cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat
    20101 cgaaccacga ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat
    20161 ccccatctca caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg
    20221 tttgatgtta cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat
    20281 agctgtacaa ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc
    20341 aatcgccatt ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat
    20401 aacttggttt tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg
    20461 gtcttgattc attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta
    20521 gattccatag atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc
    20581 gtaagggaaa tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt
    20641 agtgatcggt gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc
    20701 atttccaata ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct
    20761 ctccttttct tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat
    20821 atatggattt tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg
    20881 ctgcatcgga atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat
    20941 atcagttgtg atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc
    21001 tcgtgccaca gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg
    21061 accaatattt ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg
    21121 cttcatcatt tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc
    21181 cagacatttt ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc
    21241 ggcattgttt actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg
    21301 tcccgctttc tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc
    21361 ttcgatttct tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc
    21421 tccagctttt gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat
    21481 aaatacattt tttcctttta catccataac gttcctcctt atagtattgc tattgtttca
    21541 tcaaatgtct gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata
    21601 aatccgctta atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt
    21661 aacttatgga tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc
    21721 gcgattgttt ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt
    21781 tcagagaatt gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata
    21841 gctgtatgaa aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt
    21901 aatagtaaaa cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt
    21961 gtattataat ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg
    22021 atcgtttcaa tcggcgcatt catcactgca accattttcc cgcttccagc tggggcagct
    22081 tctgtcatat aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca
    22141 ttactagcta ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg
    22201 attccatgct ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt
    22261 gtatattgcg tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag
    22321 ccgagaatct cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct
    22381 tttcccatac cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt
    22441 ttctccaata tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc
    22501 ctatttcagg aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc
    22561 tacgtaagtc actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc
    22621 aaattcatct tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc
    22681 ttcttggatg tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat
    22741 gatttcttga attttttcga ataccataat aatatattcc tccgatattg tttgcttttt
    22801 atttttataa tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc
    22861 catagcctgt aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg
    22921 cttcatccaa taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg
    22981 tcaggaactt ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt
    23041 ttgcctgatg caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt
    23101 ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg
    23161 aaccttctga atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg
    23221 agctacttct ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg
    23281 cttctaagag aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc
    23341 aatcgattag cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc
    23401 ggatcatttt ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat
    23461 caaaagcgat agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg
    23521 tcgtgtaatc gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat
    23581 ttttctgttt tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg
    23641 ttacacatct tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca
    23701 tcatcttgga aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag
    23761 taatcattcc aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca
    23821 agtttttcag tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt
    23881 tcttgaccat ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg
    23941 ttagtcccaa ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt
    24001 tttttactaa cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg
    24061 ctacttctga agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg
    24121 tgattgacag atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat
    24181 cattgaagac actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc
    24241 gtccactaat tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt
    24301 caaaatgcaa ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc
    24361 atttaaaaca actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct
    24421 cacatatttt gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt
    24481 gtccattatt tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg
    24541 ttaaaagaaa aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga
    24601 gatggtaagg tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat
    24661 tttcatcaga ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat
    24721 tttgatttcg tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc
    24781 caacattacc gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta
    24841 catattacca ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct
    24901 tttcgtctct ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg
    24961 ccttggttgt ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa
    25021 atgacttttg aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag
    25081 ctactgactt acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa
    25141 gccggtatgc ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa
    25201 caaaagcagt ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag
    25261 agtcagaaaa agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct
    25321 ggtgacgttt ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt
    25381 caattgtcaa aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta
    25441 gaaaaagaac agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat
    25501 tagttgcttc tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt
    25561 tattggatta agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc
    25621 tgatgagtat ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta
    25681 cacttctcta cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg
    25741 accatagcct ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct
    25801 ccttttctta tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct
    25861 aaaaaaactc gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt
    25921 gatcaaagct tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt
    25981 caatgcttgg gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat
    26041 ctttaaatct ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat
    26101 acgaggagaa taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat
    26161 cactttcttt aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt
    26221 catcgtacct gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga
    26281 accatgcaat gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac
    26341 ggcttcttct ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc
    26401 ggataccggt gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc
    26461 tgccaggctt ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga
    26521 aaaaatcccc gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt
    26581 aaagagaata ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc
    26641 tgttactaaa ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc
    26701 ttctagtaga tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt
    26761 cattttttcg acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa
    26821 caaggatttt ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt
    26881 tgattcgttc aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa
    26941 ccatgtcttg ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa
    27001 gtttagcttc taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg
    27061 ctggtgcaaa gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc
    27121 ggttcaaata agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat
    27181 aagtaagccc ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat
    27241 aaagtgctac atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca
    27301 gttctgagat caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc
    27361 tggcgttagg ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt
    27421 tctcataaga aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg
    27481 actcccttaa tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt
    27541 cgataccagt aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa
    27601 taacacgttc acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt
    27661 attttacatg ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat
    27721 acatcgtatg gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa
    27781 gtatccctgc tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa
    27841 ccaaataagc ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg
    27901 gtacacttgg cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg
    27961 taaaaatata gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg
    28021 tagcaactcc gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt
    28081 tcgcctccaa actagtcctt gacctgattt acttttttta atttgctaga gactttttca
    28141 ttcagtacac tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct
    28201 tttgctgttt gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg
    28261 atcagtcgga taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac
    28321 cagccattat acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct
    28381 aaaacaacga tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa
    28441 tacgcacctt ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga
    28501 tcttctttta caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac
    28561 caaataaatg tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta
    28621 cgttcgaaaa acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat
    28681 tctctgaact tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg
    28741 gaaatagctt gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt
    28801 gttggcgtga tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata
    28861 ggagaaagct ggttcgatcc gataaaggaa tttcggaaat cttcctctgt agaatgcgca
    28921 tgatagacaa ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt
    28981 ccgtatgtat tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata
    29041 ccgacttccg tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca
    29101 agcatctttt cactttcaaa atataataat accttcacct tacgtccccc ttcacttata
    29161 tagtataaca tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta
    29221 ctgacaaaaa cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca
    29281 caccagcttt tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc
    29341 catcgtatcg cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa
    29401 gcgtttcatt aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag
    29461 acccatacct actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc
    29521 tgcccatttt ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga
    29581 tgggttatat ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt
    29641 gtattgaatc aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc
    29701 tgctaagact gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac
    29761 ttccttgcct tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg
    29821 gaattctttg agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc
    29881 acgaagcaat gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat
    29941 acgcaatgca cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag
    30001 ttctttatct ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc
    30061 taatacagct ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat
    30121 atacaggaat tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac
    30181 tagatctttt ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac
    30241 agtttctgca ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc
    30301 tttttcaaat tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc
    30361 gttgacagcc aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat
    30421 tgctggaatt tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc
    30481 tgttacaaac gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc
    30541 atgtgcaaca acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa
    30601 taagtgcgct aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata
    30661 agcattatct tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc
    30721 tgattcagca tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg
    30781 atctgaaaga accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg
    30841 cgctgctttt tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa
    30901 acgagcttct tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg
    30961 aactagcagg taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat
    31021 ttcaaccatt attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca
    31081 tcagcttcgt cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa
    31141 gacataacgc ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca
    31201 tcagagttaa atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca
    31261 gtttctgcta ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt
    31321 gttgttttta tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc
    31381 ctttcatctg taaagattaa catttttttt cgttagatac aacagttttc attcaatttc
    31441 agataaaagt attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac
    31501 aaacgtgttt atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc
    31561 ctttgaccct ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta
    31621 taataagagt caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat
    31681 ctcttaaaaa actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat
    31741 gtacccattt ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat
    31801 gaggcaacga ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt
    31861 cagagctgtt atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa
    31921 aatgatccat ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg
    31981 caacagcaag gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc
    32041 aatggtggtc agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat
    32101 atcacagaac aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc
    32161 aaacgagtca ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa
    32221 ccaggtgttg gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat
    32281 gtaccgcaaa aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa
    32341 ggtactggta tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt
    32401 caagcagaaa atgtgatcct attcatagat gaagttcacg aaatagtcgg tgctggttct
    32461 gctggtgatg gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa
    32521 ttgcaaatgg ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct
    32581 ttagaacgtc gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt
    32641 attttgaaag gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag
    32701 gccatcgaag cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat
    32761 aaagccatcg atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt
    32821 gatcctaaaa caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct
    32881 cgagaagaag atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa
    32941 atgaaagaac gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa
    33001 aaaatcgttg aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca
    33061 caactgaaaa acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta
    33121 gatcgagtag cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca
    33181 atcggctcct tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa
    33241 cttgcctacg aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat
    33301 atggaaaaac atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa
    33361 gaagctggtc aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat
    33421 gaagtagaaa aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt
    33481 cgattgacag atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca
    33541 agtaatgccg gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt
    33601 gttactcgtt ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt
    33661 tttgacggga tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca
    33721 ctaatgctag acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct
    33781 acgaatgtca aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct
    33841 cttcgacgca cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac
    33901 ccttctatcc atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct
    33961 aaaccagaac gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat
    34021 agcgtgagac ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa
    34081 tactgttcat tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata
    34141 aaagaggtga gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta
    34201 aaccagcttg aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact
    34261 attgtctaca ctgaagcccc tcaacataat gaaattctgc tgatcaatga caaacgtaaa
    34321 atccagccga aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc
    34381 gtttaccacg aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt
    34441 ccaaaacatg aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa
    34501 agagcgtggg acaaaagtaa a
//
LOCUS       DAZKOB010000018        31762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000018 DAZKOB010000000
VERSION     DAZKOB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.018"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..540
                     /gene="mraZ"
                     /locus_tag="ACG8M6_000901"
     CDS             109..540
                     /gene="mraZ"
                     /locus_tag="ACG8M6_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0456590.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            561..1520
                     /gene="rsmH"
                     /locus_tag="ACG8M6_000902"
     CDS             561..1520
                     /gene="rsmH"
                     /locus_tag="ACG8M6_000902"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0456591.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1525..1926
                     /gene="ftsL"
                     /locus_tag="ACG8M6_000903"
     CDS             1525..1926
                     /gene="ftsL"
                     /locus_tag="ACG8M6_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0456592.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            1927..4119
                     /locus_tag="ACG8M6_000904"
     CDS             1927..4119
                     /locus_tag="ACG8M6_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0456593.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4145..5107
                     /gene="mraY"
                     /locus_tag="ACG8M6_000905"
     CDS             4145..5107
                     /gene="mraY"
                     /locus_tag="ACG8M6_000905"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0456594.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5121..6482
                     /gene="murD"
                     /locus_tag="ACG8M6_000906"
     CDS             5121..6482
                     /gene="murD"
                     /locus_tag="ACG8M6_000906"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0456595.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6508..7596
                     /gene="murG"
                     /locus_tag="ACG8M6_000907"
     CDS             6508..7596
                     /gene="murG"
                     /locus_tag="ACG8M6_000907"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0456596.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            7611..8831
                     /locus_tag="ACG8M6_000908"
     CDS             7611..8831
                     /locus_tag="ACG8M6_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0456597.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            8987..10312
                     /gene="ftsA"
                     /locus_tag="ACG8M6_000909"
     CDS             8987..10312
                     /gene="ftsA"
                     /locus_tag="ACG8M6_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0456598.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10335..11576
                     /gene="ftsZ"
                     /locus_tag="ACG8M6_000910"
     CDS             10335..11576
                     /gene="ftsZ"
                     /locus_tag="ACG8M6_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0456599.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            11593..12270
                     /locus_tag="ACG8M6_000911"
     CDS             11593..12270
                     /locus_tag="ACG8M6_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0456600.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12284..12883
                     /locus_tag="ACG8M6_000912"
     CDS             12284..12883
                     /locus_tag="ACG8M6_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0456601.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            12964..13185
                     /locus_tag="ACG8M6_000913"
     CDS             12964..13185
                     /locus_tag="ACG8M6_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0456602.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13234..14016
                     /locus_tag="ACG8M6_000914"
     CDS             13234..14016
                     /locus_tag="ACG8M6_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0456603.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14037..14744
                     /locus_tag="ACG8M6_000915"
     CDS             14037..14744
                     /locus_tag="ACG8M6_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0456604.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15006..17804
                     /gene="ileS"
                     /locus_tag="ACG8M6_000916"
     CDS             15006..17804
                     /gene="ileS"
                     /locus_tag="ACG8M6_000916"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0456605.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(17853..19379)
                     /gene="zwf"
                     /locus_tag="ACG8M6_000917"
     CDS             complement(17853..19379)
                     /gene="zwf"
                     /locus_tag="ACG8M6_000917"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0456606.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19394..20041)
                     /locus_tag="ACG8M6_000918"
     CDS             complement(19394..20041)
                     /locus_tag="ACG8M6_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0456607.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20225..20554)
                     /locus_tag="ACG8M6_000919"
     CDS             complement(20225..20554)
                     /locus_tag="ACG8M6_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0456608.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            20731..21459
                     /gene="sfsA"
                     /locus_tag="ACG8M6_000920"
     CDS             20731..21459
                     /gene="sfsA"
                     /locus_tag="ACG8M6_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0456609.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21475..22488
                     /locus_tag="ACG8M6_000921"
     CDS             21475..22488
                     /locus_tag="ACG8M6_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0456610.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22488..23765
                     /locus_tag="ACG8M6_000922"
     CDS             22488..23765
                     /locus_tag="ACG8M6_000922"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0456611.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            23828..26530
                     /locus_tag="ACG8M6_000923"
     CDS             23828..26530
                     /locus_tag="ACG8M6_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0456612.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            26682..26999
                     /locus_tag="ACG8M6_000924"
     CDS             26682..26999
                     /locus_tag="ACG8M6_000924"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0456613.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            27029..27349
                     /locus_tag="ACG8M6_000925"
     CDS             27029..27349
                     /locus_tag="ACG8M6_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0456614.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27457..28917
                     /locus_tag="ACG8M6_000926"
     CDS             27457..28917
                     /locus_tag="ACG8M6_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0456615.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            28985..29206
                     /locus_tag="ACG8M6_000927"
     CDS             28985..29206
                     /locus_tag="ACG8M6_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456616.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29237..29419
                     /locus_tag="ACG8M6_000928"
     CDS             29237..29419
                     /locus_tag="ACG8M6_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0456617.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29419..29832
                     /locus_tag="ACG8M6_000929"
     CDS             29419..29832
                     /locus_tag="ACG8M6_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0456618.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            29955..31136
                     /locus_tag="ACG8M6_000930"
     CDS             29955..31136
                     /locus_tag="ACG8M6_000930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0456619.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(31207..31371)
                     /locus_tag="ACG8M6_000931"
     CDS             complement(31207..31371)
                     /locus_tag="ACG8M6_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456620.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            31525..31598
                     /locus_tag="ACG8M6_000932"
     tRNA            31525..31598
                     /locus_tag="ACG8M6_000932"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31559..31561,aa:Arg,seq:cct)
     gene            complement(31667..>31762)
                     /locus_tag="ACG8M6_000933"
     CDS             complement(31667..>31762)
                     /locus_tag="ACG8M6_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0456621.1"
                     /translation="MSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        10664 a         5454 c         6775 g         8869 t
ORIGIN      
        1 aatgtttatg aataaaaaaa gttgttcccg gatttcccag aaaaggtggt agaaagtggg
       61 ggattgtggt agactaattt tagttagtgg aggaatgggg ggctttcaat gttgatgggt
      121 gaattccaac ataatattga tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa
      181 gaacttggcg aaaagtttgt tctgacacgc ggattggatg gctgtctttt cggctatccg
      241 atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac
      301 gcacgtactt ttgtgcgatt cttctattcg gctgccactg aatgcgaact tgataaacaa
      361 ggacgaatca atatacccag cacgttaaga aattatgcag ctttgacaaa aggttgcgta
      421 gtcatcggtg tatctaaccg aattgaaatt tgggatgaga cacgatggca agatttttct
      481 gcagcagcag aagagaactt tgacgaaatt gctgaaagta tgattgattt cggactataa
      541 gaataggaag aggctgacta atgactgaaa catttcaaca ctatacagtc atgttgaaag
      601 aaacggtaga tggattgaac atcaaaccgg atggcgttta tgtagactgt acactcggcg
      661 gtgccggaca tagtgaatat ttattaagcc aactaagttc taaagggcat ctgtatgcgt
      721 ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg tttagcttct tatgtcgaac
      781 aaggtatggt cacatttatc aaagcgaact tcagagaatt agaagaacgc ttgagtgagc
      841 atgtagataa ggtagacggc attttgtatg atctaggggt ttcctctcct cagctagatg
      901 aagcagaacg tggatttagt tatcatcaag atgcaccgtt ggatatgcgc atggatcagt
      961 cagctccttt atccgcttat cacgtaatta atgaatattc ctaccatgaa ttagtaaaga
     1021 ttttcttccg ctatggcgag gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag
     1081 tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt cgaaatcatt aaacaagtca
     1141 tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa aagaattttt caagctgtgc
     1201 ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc tttagaacag gcaatccggt
     1261 tattgaaagt caatggaaga atcagcgtaa tcacattcca ttcattggaa gacagaattg
     1321 tcaaaagtat gtttaaagaa tatagtacgg tacaagatct accaccggga cttcctgtca
     1381 tacctgacga attccaacca gaactgaaag tcattactcg taaaccaatc cttccttcag
     1441 aggaagagct aacagaaaac aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa
     1501 taagaatgaa agaggagtag aatgatggca gaattaaaga aaatgcaaga ttacgaatat
     1561 gatttacctg aattggtgga tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa
     1621 gaaatcattc ttccacaatc accggcaaaa cgattaaagc agatttctgc tttggaaaaa
     1681 attgctgtgg caagcgtggc gattgctatt gttgctttat gtgttttaac agttatgctg
     1741 cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat
     1801 aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg aactttctcg tactgaacgg
     1861 attaaacaaa tcgcagaaga aaaaggattg tctattaatg acgacaattt aaggaaagtg
     1921 aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg
     1981 atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta cgagtattgg attgttcttt
     2041 ttatttgccg tccgtttctc gtatatcgtt attggaggtc atgttgctgg tacctcatta
     2101 gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag taaaagcaaa aagaggaacg
     2161 atttatgatc gtaacggtgt ggcgctcgca gaagatgcct cctcttattc aattaaagcg
     2221 atactctcaa aaacatatac ctctggggat aaaaaactgt atgttgaaga aaagaatttt
     2281 gataaaatag cagaaatcct gcataagaat ctttctatag acaaaaaaga tgcactgaat
     2341 attttagagg acggcgcaaa aaaagagctg tatcaagttg aatttggtag ctatgggaaa
     2401 aatatctccc aagaaaccaa gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct
     2461 ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa atggcgtctt ttcttctcat
     2521 tttattggat acgcagttcc agacaaagat gagaatggct tagttggtaa actaggtttg
     2581 gagtctgctt acaatgatat cttgagcggt aaagatggta agattattta tcaaaaagat
     2641 aattttcaaa atcctctgcc aggtacagtg gcagaagaag aaaaagctgt cgacggacaa
     2701 gatatctaca cgactttaga cagcagacta caaagttatt tagaaacact gatggatcaa
     2761 gtaaatgaag aatatcagcc agaagaattg acagcagtcc tgatgaaagc aaaaaccgga
     2821 gaaatcttgg caatgggaca acgacctact ttcaatcctg aaacaatgga aggattgact
     2881 ggaaaagatg ctatctggcg taatttcttg gtacaagata gttatgaacc aggatcaacc
     2941 atgaaagttt tcactactgc agctgctatc gaagaaggag aattcaacga gaatgaaacc
     3001 ttccaatctg gaaaaatcca agtagaagat gctacgatca atgaccatga ctttggtgaa
     3061 aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata
     3121 ctggaacagc gattaggcgg acgatggtat aactatttac aaaaactggg atttggacaa
     3181 agtactcatt ctggattaga tgatgaagta aacggtgctt tacctacatt aaatattgtc
     3241 gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa
     3301 gctttcactt ctattgcgaa taacggtaca atgattcaac ctcgatatat cagcaaagtg
     3361 gtggatcccc aaactggaga agagcgaaca actcaaacag aagttctagg gcaacctttt
     3421 tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag atgttgtcga aagcgaaaac
     3481 tacggtagtg cttatggtgt atatagtgta cctggataca atgtatcggc aaaaaacgga
     3541 acagcccaaa tcgcttctga tacaggggga tatcaaacag gagatacagc ctacctgtat
     3601 tcgattgtgg agatggttcc ttctgaagat ccagactatg tcttgtatct aacaatgaaa
     3661 caccctaaga catatgatcg aatggcattg gctaaaatcg caaatccatt gatgaaacgg
     3721 gcaatggatt ttaaagaaac tgaagaagat tcagatacag aaacaaaaac agaaaaagta
     3781 tccgtagccg attatcgtaa tcttgaagcg gacgttgcag cagctgatgc tcaaaaaagt
     3841 ggactgcaac ctgtagtgat cggtaatggt aaaaaagtac aaaaacaatc tactgccaac
     3901 ggtgatcagt tgatctctgg agaaaaactg atcctatata ctggtggtga taagctgatg
     3961 cctgacgtaa caggatggtc aaaagcagat atcatgaagc tggggaaaat cctcggtatt
     4021 gaagttagtt ttgatggaga tggttattgt gtcaaacaag aacttgctcc atatgaaaaa
     4081 atcactgaag acaaacttag ttttacttta gaagaataag aagagaaata ggagagaaaa
     4141 gagcatggaa tggacacaag cattgattcc cattgtcagc agttgtgcaa tgacaattgc
     4201 cgcaatgccg ctatttattg gttattttca aatgaaaaag caaggtcagg cgattcgtga
     4261 ggagggacca aagtggcaca atagcaaagc cggaactcct acaatgggcg gcttagtgtt
     4321 tctgatcggc tctattttga caggaatttg ggttggagct tggcaaaaac agctaactcc
     4381 tactcttttc atcttgcttt ttgtattagc gttatacgga gtaatcggct ttcttgatga
     4441 ttttataaaa atattcaaga aaagaaacat gggactgaac tcgaagcaga aattgattgg
     4501 acagattcta gggggaatca tcttttatct tgtttaccgt tctgaaggtt atccaggtgt
     4561 attgaatttc ttcggactag atctaccttt aggaatcatt tatggtattt tcgctctctt
     4621 ctggcttgtt ggattttcaa atgcagtaaa tctgacagat ggcatcgatg gattagttgc
     4681 tggcttagga agtatctctt ttgcagctta cggtctaatt gcttggcatc agcatcagta
     4741 tgatgtatta atcatttgct taagtgtttt aggaggttta ctgggtttct tcgtctacaa
     4801 cagaaaacct gccaagattt ttatgggaga tgtcggttca ttagcgttgg gcggactttt
     4861 agcggcaatt tctataatgc taaaccaaga atggacattg cttttgattg gtttgatcta
     4921 tgttatggaa acagcaagtg tgatgcttca agttacttcc tttaagctta caggaaaacg
     4981 aatttttaaa atgtctccaa ttcatcatca ctttgaaatg tgcggatggt cggagtggaa
     5041 gattgataca gttttttggt tgatcagcat tgtgacttcg ttgatcacat tatggatcgt
     5101 ttggtaggag atgacgggaa atgaaaaaaa tatcaacata tgaaaataag aaagtgcttg
     5161 tcttaggact cgcaaaaagc ggagtaagtg cggccaaatt attacatgaa ttgggcgcac
     5221 ttgtcaccgt taatgatgga aaaccttttg acgaaaatcc ggaagcacaa gagctgcttt
     5281 ctttagggat aaaagtaata acaggtagtc atccaatcga attgttggat gaagaatttt
     5341 cattgatggt caaaaatcca gggattccct acagtcatcc tttagtagca aaagcccaag
     5401 aaatgggtat tccggtaatt acggaagttg agctagctta cgaagtagcg gaatgcccaa
     5461 ttattgggat tacaggaacg aacggaaaaa caacgacaac aacgatgaca ggactactac
     5521 tgaatgcagg agcagatcaa ggaattgcac gtttagcggg taatattggg tatccagcaa
     5581 gtggtgtagc gcaagaagca aaatcagaag ataaaattgt gatggaattg tctagcttcc
     5641 aactcatggg aatcacagat ttccgccctc atatagcggt gattaccaat atctatgaag
     5701 cacatattga ttatcatggt acaagaaaag aatatgtcaa agctaaatgg aaccttcaaa
     5761 aaaacatgac agaaaaagat tatctgatcc ttaactggaa tcagtcagaa ctacaagaat
     5821 tagcgcaaag aacaaaagca cgcgtactgc ctttctcgac aaaagaagtg ttggaagacg
     5881 gtgtgtatgc tgacgattac tcaatatact ataaaaaaga aaaaataatg gaaatatctg
     5941 aacttggcgt accaggtaag cataatgtag aaaatgcact agctgctatt tctgtagcaa
     6001 aattgtatgg tatatcaaat gaagctatac gagaaacgtt gcattttttc catggcgtac
     6061 cacatcgtac gcaatatgta ggagaaatcc aaggcagaaa attttataat gattcaaaag
     6121 caaccaatat tttagcgact aaaatggctc taagtggatt tgaaacgtct aaggtcgtgt
     6181 tattggctgg cggattagac agaggaaata catttgatga attaattcct tcattaaaag
     6241 gaatcaaagc aatggtcgta tttggccaaa caaaagaaaa gttgatggat gctggaaaaa
     6301 aagcaggcat cgagacaata gtaacagccg attctgtaga acaagcagtt cctttagcat
     6361 tagaaaacag tacagatggt gatgtagttt tattatcacc agcgaatgcc agctgggatc
     6421 aatacccaaa ttttgaaact agaggaaaca gattcatgga agctgtgaac cgattaaaat
     6481 agaaaaagta gaagcgagcg ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg
     6541 ggacatattt atccagctct tgcctttgtc aattatgtaa agactcaaga accgaatgct
     6601 gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga
     6661 atgcctttcc ataccttgga gattcaagga tttaaacgaa aaatttccat gcataacatc
     6721 aagacggtcc gattgtttct taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt
     6781 caaccagata tcgttatcgg tactggagga tatgtttccg gagcggtagt gtacgctgca
     6841 gccaaaatgg gtattccaac agttatccat gaacaaaaca gtgtaccagg tgttaccaat
     6901 aaatttttaa gccgatatgt ggatcggatc gctctttcat ttaaagatgc ggcttccttt
     6961 tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag cacaagaagt agcagatact
     7021 aaaaaatcag atattttgaa aacatttgat ttggatccag aaaagaaaac agtcttgatt
     7081 tttggcggta gtcaaggagc acttaaaatc aatcaagcag tatcagtttt tttaagtgaa
     7141 tatattcctt cttcttacca agttttgtat gcttcaggag atagatatta tgaagagatc
     7201 ctaaataaaa taaatcctga aatacaaaat attagtgtcc agccttatat caaaaatatg
     7261 gcagaggtca tggcttccag tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca
     7321 gaattcacag ctctggggtt accggcagtg ttggttccca gcccatatgt tacgaatgat
     7381 catcagacta aaaatgcaat gagtcttgtt cacgcaggag cagcaaaaat gattgcagat
     7441 aatgaactaa caggtgaatc actaagtcag acagtcaacg aaatcatggg tgatgaagaa
     7501 ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt
     7561 ctttatgact tagtaaaaca aataatcaaa aaataaaggc ggtggtctta gtgagtaaga
     7621 aaaaagaacc aaaaaaattg tctcatccgt cttctgatga aaacaatttg actccttggc
     7681 aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag
     7741 aagacataac attagataca cccctttcag attctgaaga agaaaaaacg gttcaagagg
     7801 aacagcaaga agaaagccag tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa
     7861 agaaaaacgg tggaccatat aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc
     7921 aacggcacaa agtactattt cgtcggttag ctttgatcat cactgtattg ggtataccgc
     7981 tgattttttt gatctattat gtttctcctt atagcaaatt acaagcagtt gcagtatcag
     8041 gcaataagac agtcaacagc caagaaatca tctctgatac aaaattgtca ttaggagaaa
     8101 atgtttgggg acagtatttt catcgatcta cttatatcga tcgccttaaa aaagcacagc
     8161 caagaatcga aacagcaaat atacatttca aagggatgaa tgaattcgat ttggatgtaa
     8221 cagaatataa agagattgct ttgatcgcaa aaaacaatca atactaccca gtgattgaaa
     8281 atggaacagt attagatgaa aaagtagcaa atcctacaaa aaatctccct atcttggaag
     8341 agttcaaaga ctctgcaaaa atcaaagaat taacaaagca atacaatcag ttatcatcag
     8401 aattacaaaa agcaatttct gaaatcaagt acacgccaag agcaagcaat aaaaatctga
     8461 tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc
     8521 aaatgaaata ttattcgcaa gtagccaaag atatggatga aaaaggtgtg atcgacatgg
     8581 aagttggtat cttctcgcat ccttacggta gcactcaaga aacggaagaa tcgggccaat
     8641 cggaagaaac aactgaaagt gaagaaaatg cggatcaaac aaacgaatca agtgaagagg
     8701 cagaaacgca aaaagcacaa acacctggat cgtctagcac aaacggatca tctgattcaa
     8761 atgattcaag tgaaacaagt caaatagaag ataatccaag tacaactccg tcaagtttaa
     8821 atgaaaattg ataaaacaac tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt
     8881 atggtagaat tggaagcagt gaatatatct attaataagt atattttaaa acggacgcta
     8941 gttgttcgct tgtatatgga atagtaagta ggagggaatc ccattcatgg caaaaacagg
     9001 aatgtatgta ggccttgata ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat
     9061 cgacagccaa atgaatatta ttggagtagg aaacgcaaaa tcagaaggga ttaaccgagg
     9121 cattatcgtt gatatcgata aaacggtcca agcgatacaa cgagcagtac gacaagcaga
     9181 agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga
     9241 agtagaaaat tgccagggaa tgattgcagt taacggagat tcaaaggaaa tcaccgatga
     9301 agatgtcaga aatgtggctt cagctgccct tgttcgttca atacctcctg aacgtcaaat
     9361 cgtgtcaata ttgccacaag attttactgt cgatgggttc gagggaatca aagatcctcg
     9421 tggaatgatc ggcgtaagat tagaaatgta cggtctgttg tttacaggac ctaaaacgat
     9481 catccataac atacgtaaat gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat
     9541 tactccttta gcgctgactg aatcaattct ttctgacgga gaaaaagatt ttggtacgat
     9601 cgtcattgat atgggtggcg gccaaacaac aacggctgtt atgcatgata agcaattgaa
     9661 gtttaccaat cttgaccaag aaggtggcga atttgtcact aaagatatct ctattgtatt
     9721 aaatacgtca tttaacaatg cagaagcgtt gaaaataaac tacggggatg catacccaga
     9781 acggacatca ccagatgaag aatttccagt agatgtgatt ggtcagtcag aaccagtgaa
     9841 agtcgatgaa cgctacttgt cagaaattat ttcagctcga atggaacaga ttttcaacaa
     9901 agcaaaagat gcattggacc aaatcgaagc attagagttg ccaggtggcg tcgtattgac
     9961 aggcggagct gctagtcttc ctggtgttgt ggacttggca caagaaatct tcggcgtaaa
    10021 tgtaaaattg tatgttccaa atcacatggg gctgagaaac ccggtattta caaatgtaat
    10081 aagtatcgtg gattattcag caaacttgag tgaagtgtat caattggcaa aaattgctgt
    10141 tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa caagaaacaa caaacacata
    10201 tgagagctat gaagcacctg aagaaacaat ctacgatgag ccggaaccaa aagagtccgg
    10261 agaaaatgta aaaaataaaa tcaagggttt ctttacaaat atttttgatt aattcatcct
    10321 gggaggaata agatatggaa ttctcaattg ataataatat caatgacggc gcagtaatca
    10381 aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg
    10441 ttaaaggtgt tgaattcatt acagccaaca cagacgtgca agcattaaaa aattcaaaag
    10501 ctgaaacagt tatccaatta ggccccaaat atactcgtgg tttaggtgct ggttctcagc
    10561 cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg
    10621 gtgcagatat gattttcatc actgcaggaa tgggtggagg aaccggtaca ggtgctgcac
    10681 caatcgttgc aggtatcgcg aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc
    10741 cattcacttt tgaaggaccg aaacgtggcc gttttgctgc agaaggtatc gctcgtttga
    10801 aagaaaatgt ggatacatta ttaattatct caaataaccg tttattagaa gttgtagata
    10861 aaaagacacc gatgcttgaa gctttccgtg aagcagataa tgttttacga caaggtgtac
    10921 aaggtatctc tgatttgatc acagcgccag gttacgttaa ccttgacttt gctgacgtga
    10981 aaacagtgat ggaaaaccaa ggtacagcat tgatggggat cggtgtggct agtggagaag
    11041 atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc attgcttgaa acatctattg
    11101 atggagctga acaagtactt cttaacatca caggcggttt ggatatgaca ttgttcgaag
    11161 cacaagatgc ttcagatatc gtagcaaatg cagcaactgg cgatgtaaat atcattctag
    11221 gtacatctat caatgaagaa atgggcgatg aaatccgtgt gacggtcatc gcaacaggta
    11281 tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc tagacaagca caaatgcaat
    11341 cacctgctca aaagactgta ttagatatgg atcaagctaa accaacttct tcagaagaag
    11401 aaaacagttt tggcgattgg gatatcagac gtgaacaaaa tgttcgtcct agagttgatg
    11461 attccaattt tgacaatatc gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa
    11521 aatctcatgg cgatgatgaa ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga
    11581 gaggaagagc acatgattgc tgacaacttg cagaaaatca agcaagaaat tcaggattcg
    11641 tgtgctcttg ttcatcgtga tccgaaagaa gttacattag tagctgtaac aaaatctgtt
    11701 gaatccgctc aagctgaaga attggttaga gctggcgtta tgaattgtgc agaaaaccga
    11761 gtagataaat tattagaaaa gaaacaagat ttatttgatt ttcctgaaat aaagtggcat
    11821 ttaattggta atttacagcg cagaaaggta aaattgattg taaatgaaat tgattacttt
    11881 catgcgttgg acagtttaag attagcagaa gaaatccaga agcggttaga gaaagaatta
    11941 tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa aacatggaat ttcaccagat
    12001 gagcttttac cgtttgtgga atctttagct gcctgcacaa aaatcaaggt tgttgggttg
    12061 atgactatgg cgcctaaaga agcttctgat gagcaaatcc ataaagtgtt tggtacgctg
    12121 aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta
    12181 agtatgggga tgagtcaaga ctttcaaatt gcgattgaag aaggtgcaac atttatccgt
    12241 gtaggaacag cgctattcaa agacgaataa aggaggaaca aaaatgccgc ttttaaacag
    12301 aggagcaatt gctaattttt tcggattagc tgatgaggaa gagtacgaat accaagatta
    12361 tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa aatacaacac agaatcaatc
    12421 aactcattct gtatctgctg cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc
    12481 tgtaacgcat gaaagtattc agccggctga aaagaaaaaa gtaattgaac tacatcaatc
    12541 acctacaaaa actgtagcga aagaaagaac agcttcaaga acctctacac aacctaaagc
    12601 aaatcttgct ggaaaaatca ctgtaatgga accaagagct tattctgaag ctatggcgat
    12661 tgctaaacga atcattgctg gagaagcaac cttagtgaat tttcatttag tagaagaaaa
    12721 acaagcgaga agaatcgttg attttctaac aggaacagtt tatgcactag atggagatat
    12781 caaacgtgtt ggagatgaga tttttctttg tacaccgact gggatggaga ttgacagttc
    12841 aaccgctcaa tcttttgcag atgcgaattt gtttgatctg taagaggagg aactacgatt
    12901 tatactattg cactattact tcatttactt caacgggcag cctatttcta tagcattatc
    12961 ttgatggtat atgcattgct ctcttggttt ccaggcgggt atcaatcagg gctaggacga
    13021 ttattagcta agatctgcga accgtatatc agtttgttcg atcgtctgcc tttgaggatt
    13081 ggacccttgg atttcacaat tttaggggca atcctgatct tgaacttagc cgtacaaggt
    13141 ttagcacaga tcgttatatg gataactgtc catttagttt attgattgaa gcaacaaaat
    13201 ctttattctc agaatagata ggaggggaaa attttggatg caaatgtata ccagcacttt
    13261 cgggcagatg aacgtacatt tatcgattcg gtaggcgatt ggattgaaca ggttcaagga
    13321 caatatgcac cctatctgac cgaatttctt gacccaagac aatcctatat tttagaaaca
    13381 ctgattcgac aaagcagtga tttgcgtttc atgttttatg gcggatatga acaggcagaa
    13441 agaaaaagat gtttgatttt ccccgattat tacgaacctg tagaggaaga ttttgaaaca
    13501 gcattgtatg aaatccatta tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt
    13561 ggaactttag tgggaacggg gatcaaacgc tcttactttg gcgatatcat ctcggacggt
    13621 gatcgctggc aagtatttat tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca
    13681 aagattggaa aggttgctgt acgcttggaa ccaatcaaat acacagaaat gattatgccg
    13741 aaagatagct ggacacaaga aagaggaaca gttagttcca tgcgactaga cagtgtgatc
    13801 tctgagattt ataatatttc cagacagcgg tccaagcagc tgatcgaagc tggtaaagta
    13861 aaagtgaact ggacagaaaa tacaagacct gattttgtac ttgaactatt agatattatt
    13921 tcagtcagag gttatggcag agtccagatc caagaacttg aggggaagac gaaaaaagaa
    13981 aagtacaggg tactgttcgg tgtattacgt aaataaaata aaaaggtggt ttactcatgg
    14041 ctttaactcc attagatatc caaaataaga cattcccgac aaaaatgcgg ggatataatc
    14101 aagacgaagt agatgatttc ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa
    14161 gaaatcgaga attagaaaaa gcagtcaaac attctgaaga aaaacttgag tacttcaatg
    14221 aattaaaaga tgctttgaac caatcaatca tcgtagctca agatacggct gataaagtaa
    14281 aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc tgcacaaaac aaagcagatg
    14341 agttagttgc gaacgctgaa aaacgtgcac atcaattaac aactgatgca gaagaaaaag
    14401 caagaaaaat cttgacagat gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag
    14461 atcttaagaa aaaaacacga gtattccatc aacgtatcag cttgatgctt gagtctcaat
    14521 tagaacaagt gaaaagtccg gaatgggatg aaattttaca accattctcc agctacgtga
    14581 ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca gcttgacaat gaaaatgata
    14641 ctgatgtaaa ctcagacaca ggagtcatag aagcacctat gacggcattt gatacacagg
    14701 ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga gtagaacaga aaagcacttt
    14761 gttattctta aagcgagccg atgagagtgg aagttcggta agacactgca tggtgaaaga
    14821 tcctctactt gagacctgct tttccgaaat tagagtaaga agcagcggct gacctcgtta
    14881 ttggtcacag agagtttgca ctagatagat gcaactaaat ttgggtggta ccacggcatg
    14941 ttcgtccctt gcggctgaac atgctttttt gtgtccggaa aacaaataaa ggagtgtatg
    15001 gttgaatgaa aatgaaagaa accttgcaat taggaaaaac aaaattccct atgcgtggta
    15061 atttgccaaa ccgagaaggc gagtggcaaa aagaatggga agaaaatcga atctaccaaa
    15121 aaagacaaga gttgaacgaa ggaaaaccta gctttgttct tcatgatgga cctccatatg
    15181 ccaatggtaa tatccattta ggacattcat tgaataaaat cagtaaagac attatcattc
    15241 gttcaaaatc aatgtctggc tatcgtgcac catatgttcc aggatgggat acacatggat
    15301 tgccgattga acaagtattg gctaataaag gcgtgaaacg taaagaaatg tccatggcag
    15361 aatacttgga aaaatgtcgt gaatatgcac tttcccaagt agataagcaa cgagcagatt
    15421 tcaaacgcct aggagttgca ggagattggg aacatcctta tgttacatta gatccttctt
    15481 atgaagctgc acaagtacgc gtatttggta aaatggctga aaaaggctat atttacaaag
    15541 gattgaaacc aatttactgg tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg
    15601 aatataaaga tgtaaaatct ccatctattt ttgtagcttt caatgtcaaa gacggcaaag
    15661 atattcttga tgaagataca gcttttgtca tttggacgac aacaccatgg acattgccag
    15721 caaaccaagg tattgctgta aatccaaaat ttacgtatgt attggtggaa gccgatggcc
    15781 gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca atcagaaatc ggttgggaaa
    15841 atgtaacagt tctaaaagaa tttgctggac aagaaatgga atatatgact gcaagtcatc
    15901 cattctatga acgtgaatca cttgtgattt taggcgatca tgttacttta gacgctggta
    15961 ctggattagt ccatactgct ccaggacatg gggaagatga ctatatcgct ggtagcaaat
    16021 ataaactatc tgtagtgtca cctgtagact caaaaggatt gtttacagaa gaagcacctg
    16081 gatttgaagg tatcttttat gacaaagcaa atcctatgat cactgatcta ttaaaagaaa
    16141 aaggagcttt gctgaagcta gatttcttta ctcatagtta tcctcacgat tggcggacga
    16201 aaaagcctgt gatctatcgg gcaactccac aatggtttgc atctatcgat aaattccgtc
    16261 agaatatttt ggatgaagta gaaaaagtag agtgggttat cccatggggt aaaacacgtt
    16321 tgtataatat gatccgtgac cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag
    16381 taccacttcc gattttctat gctgaaaatg gagaagcaat catcacacca gaaacaatcg
    16441 aacatgtggc aaaattattt gctgaacatg gatcaattat ctggtttgaa aaagaagcga
    16501 aagaacttct tcctgaagga tttacccacc caggttctcc taatggcgaa tttacaaaag
    16561 aaaaagatat catggatgtc tggtttgatt ctggttcttc tcatgaagct gttttacgtc
    16621 agcgaccaga attatcattc ccagcagata tgtatttaga aggatcagac caatatcgtg
    16681 gctggttcaa ctcaagtatc acgactagtg tggctatcaa tggtgtagca ccatacaaat
    16741 cagttatttc tcaaggattt gctttagatg gtgaaggccg taagatgagt aaatctttag
    16801 gaaacatcat tgcaccagaa aaagtgatca aacaatttgg tgcagatatc cttcgcttat
    16861 gggtatctag tgtagattat gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag
    16921 tggcagaagt atatcgtaaa atcagaaata cgatgcgttt cttattagcg aatacagaag
    16981 atttcgatcc taaaaaagat actgtttcgt acaatgattt acgttctgta gacaaatata
    17041 tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga agaagggtac gacaaatata
    17101 acttcatgca tatttaccgt acagttatga acttcttgac ggttgatcta tcgtcattct
    17161 acctggattt tgctaaagat gtagtttata ttgaagcaga agatgattat caacgtcgtt
    17221 gcatgcaaac tgttttctat caaacagctg tagctttgac aaaattattg actcctatca
    17281 ttccgcatac agcagaagaa atctggtcat tcctgaaaga agaagaggag tacgttcagt
    17341 taagcgagtt tcctggttat gaagagtttg caaaccaaga agaattgatg gatacctgga
    17401 ctgctttcat ggacttccgt gataatgtct taaaagcatt ggaagaagca agaaacagca
    17461 aattgattgg taaatctttg gaagcaaaac taactgttta tccaaaacaa caagttcgtg
    17521 aaatgttaaa agctttggat gcagatattg cacaattatt gatcgtttca ccagactact
    17581 ttgaagttat ggcagcagat gaacaagtgc cagataacgc tatggtattt gatgatgttg
    17641 ctattacagt ggaaaaagca gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag
    17701 atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag
    17761 acttctatcc tgaagctgta gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg
    17821 tgacatgttc tttgtcacac ccccttttgt tatcaatcgt ttaattttcc acgttcacga
    17881 taccagttat ctggttgcca gatccattcg aatccgtctt tttcaagtag ttcgaaagct
    17941 gctttaggtc ccattgatcc agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa
    18001 gcttttcgaa tgacatccac gatatgccaa gattgagcga cttcttccca atgagagaag
    18061 ttcgttccat caccattcaa cgcatctaat agtaattttt cgtaagcttc tgggctattt
    18121 tcaaccattt ctgcactatt tctaaattcc aatttgattg gttccgtttc aaatccttgc
    18181 cctacttctt tcccattcaa ggataaagaa aatccttcag ttggctgaat atagatcgtt
    18241 agaacattag gtggaagtgt cgtatcgtca catggttcat cgacagatgt tttgaataca
    18301 ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc ctttttcagt caaacgtttg
    18361 cctgtacgta catagaaagg aacacctgac caacggaagt tatcgatagt gaatttgcct
    18421 gcgacgaatg tttcagtctc tgattgagca tccactttgt cttcttcgcg atagcccttg
    18481 aattgtacac catccaattc accttgagca tattgcccgc gaacgaagtt ttctaacact
    18541 tcttcttcgt tataaatgcg gatagctttc aatgctttga tcttttctgt acggatttct
    18601 ttttcggaaa atgctactgg cggttccatt gcaagtaatg ctacaacctg taaaatatga
    18661 ttttgcacca tgtctttaag tgcgccacta tgatcgtagt agcccccgcg atcttctaca
    18721 ccgaggtttt cgccaaaagt aatttggata ttgtcgatat agcgattgtt ccattgagcc
    18781 tcaaaaatat tattggcaaa acggatagct gagatgtttt ggatcatttc ttttcctaaa
    18841 taatgatcaa tcctgaaaat atcttgttct gggaatactt ctctgatttg ctggttcaat
    18901 tcaaaagcag actggtaatc agaaccgaac ggtttttcta tgatcaaacg attgaagcca
    18961 ttttttgtca taatattttg tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt
    19021 gccatagcca aataatagac acggttaccg tttaattgat agtgtccatc taatcggtca
    19081 gacaattctt ttaatgtctg ataatgcgtt gaatcattta catcatggga ttgatagtaa
    19141 aaatggcttg caaaggtagt tgcttcttca tctgaaggat tcaatgcacg gattgtttct
    19201 ttgacaactt cacggtagtg ctcatcgctc cagggacggc gagcggttcc gattacagca
    19261 aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca aagacgggta caatttacgc
    19321 tgggctaggt cacctgtacc gccgaatatc gtaaacaata catttttttc attagacatg
    19381 tggatacact cctttatatt ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg
    19441 ggattttctt tctatcattt tggatagtga tcggtccttc ataagctgcg atgtcaacga
    19501 tctcatattc ttcgttgata ttcaggtcaa tagtgactaa gtaatccaac aattcttttt
    19561 catcaagcac tcgtgcgata cgcacttttg taccgacagg ataggactcc aatgtttgtc
    19621 ggcgttcttc atggacaact tcctcttttt taggaatcga tcctccatga ggacaatgtt
    19681 caggatgatt caaatagttt tctaaatgat ctgcaagatg ttccgaagtc acatgttcca
    19741 atacttcagc atcatcatgg acttcattcc agctatagtc taaatgttca actaaaaaaa
    19801 cttcccataa gcgatgtttg cgaatcagac tgctagcttt ttgcagtccg cttgcagtca
    19861 attgtacacc ttgataaggg gaatgttcta caagctcttc tttcacaagt ttagaaatca
    19921 tttcactgac agatgcagct gagacatcta gactagatac gatttgttta ttgttgactt
    19981 tcgttgtatc tccacctagt tctaaaatga ttttcaaata atcttctcga tttggtgtca
    20041 tattcgatca tcctttcatg gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt
    20101 aattgactat ttttctaaat aaaaaacaaa ggatttttag acgtttttac aacaaaaaac
    20161 gaaagatgat agagaagact cattctttcg tttataaagg atatatatat acattgggag
    20221 ggatctattt ttcagataac aacttatatg aagtgtctgc agattcgtca taaagagttt
    20281 gttcgggata agaaaagata agataaaaac catactgatc acgtacgact aaacgatatt
    20341 catctatttc taccacagat gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag
    20401 atgtaatctg taattgttgt ttttgctggc tatcactaaa gaaccatgta ccttgatact
    20461 ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt caagtaaata aaacttgaga
    20521 gaacggtaat gagtgaacca agaaaataga tcattttttt tatcaccaaa tcactccttt
    20581 atgagaaaat aatcgcatag acatattctt tcttctttat ttcattgtac cgaataaaag
    20641 ctaaaatttc tctaagactt tcgactggtt tttttactgt ttttctacaa attatgatag
    20701 gatacatagt aggaagaaag gtgacataat ttgaagtatc agcaagtgga aatagctttt
    20761 tttattgacc gcccgaatcg gtttatagca cattgtatga aaaaagacgg agaaataatt
    20821 attgcgcatg tcaaaaatac aggtagagga aaagaggtcc tgataccagg cgctgaagtg
    20881 gctattgtat ttgctcctgg acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa
    20941 aagaagggtg actggtttaa tatcgatagt caattgccta atcggctggc aattgatggt
    21001 attttagacg gcacgatcct tttaccagga ttaactggtc catttacggt atacaagcgt
    21061 gaagtaactt atcttcactc caaatttgat atttatttag agacggtggt cggacaaaaa
    21121 gcatttgttg aagtcaaagg tatgacactt gaaaataaag cgattggtgc ttttcctgat
    21181 gcgcccacac tcagaggatt gaaacatata ggagaattaa cgtatgccgc acaagatggt
    21241 tatgccgctt atgtcttgtt tattgctcag ttcgaacatc ttcatctggc aacgatccat
    21301 gaagaaatgc aaccagcact agctgatatg gttcgacatg cgcagcaaag cggagttcaa
    21361 atcctcgcct ataattgtca agtatcggca gatgaggtaa ctgtcaagca ggccattcca
    21421 tttgatgtag atatcccttt tgaagatcct atacaataga aagaaggtaa aataatgatc
    21481 aacttaggta ttatcggaac aaattggatt actcatcaat ttgtccaagc agctttaagc
    21541 acaaaaaaat atgaattgac agcggtatat tctagacatt tggaaaaagc acaggaattt
    21601 ggttctcatt atgaagggga tatcgaattc gcaacagatt tgaagacttt tttcaatatt
    21661 actcatatgg acaccattta tattgcttct ccgaatagtc ttcactttag tcaagctaag
    21721 caagcaattt tggcaggaaa aaatgtgata gttgaaaagc cagctttttc gacgccaaaa
    21781 gaaatggatg aaatcatcca tttggcaaat gaacacaatg tgtatttttt tgaagcagca
    21841 agaaatatcc atgaatcagg ttttaaaaag attgccgatt acttaccatt aaaagacgag
    21901 ataatcggtg caaatttcag ctatatgaaa tattcttcgc gctatgacca agtattagat
    21961 ggagaagaac cgaatatctt ctcgactcat ttctcaggtg gtgcattgat ggatttaggt
    22021 gtttaccttg tatatgcagc tgttgcttgg tttggaatgc caaaagaagt ccattatttc
    22081 ccaagaaaga tttctactgg tgtagatggt cttggatggg ggatcttacg ttatgagaat
    22141 tttgatgtat cgatccagcc aggtaaaatc ggtgattctt atttaccgtc agagatttat
    22201 tttgactcag gaacgctagt gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat
    22261 gatcgcgcgc ataaagaaat cattgatttg gcggtaacag ctgcagaaaa tccaatggca
    22321 gatgaagcag aggattttgc caatgtcatg gctcatccga aagatccagc ttggggattg
    22381 ttgtatgaag agtggatcga gctagctaga aatgtgaacc aagtgattta tgatttaaga
    22441 aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag atttttaatg aacgaatttg
    22501 tacaagtatt tcccgaaaat tggcagaaaa aatatgagga taaagggttt atctcacctt
    22561 ctcttattca acgagcagtt tttcagccac taagtaataa acaaagtata gtagcggttt
    22621 ctcctacagg ttcaggtaaa acactggctt atctttggcc tctactacta aacgtggaac
    22681 caggtgaagc aagtgcgttg gtcatttttg cttcttctca agaactagct attcaagtag
    22741 cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa agtccaatct ttggtgggcg
    22801 gagccaacgt caaacgtcag attgagggtt tgaaggaaaa accagaaatc ttgattggta
    22861 caccaggaag aatattggaa ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa
    22921 caatggtatt tgatgaagca gaccagctat ttgacgctgg gaacagccaa atcattgatc
    22981 aaattcttca tcaagctcca acagagtatc agctagcttt tttttcagcg acagcagatc
    23041 gttcccttga aagcattgaa aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag
    23101 cagaggacga ttctcgaaaa ggactgagac attacttttt acgtgtaccg atacgaaaga
    23161 aagatgacta cttgagacgt ctcacgcata tagaagattt tcatggtctg attttcttta
    23221 atcaactgac tgaattaggc gtaatggaag aaaaattgct gtatagaggt gtaccggtag
    23281 gaagtctggc ctctgatcag aacaagctac tccgaaaagc agcgctagaa caattcaaaa
    23341 agaaaatggt cagagcactt ttgaccacag atgtggcggc tagaggttta gatattacag
    23401 gtcttcctta tgtagtgaat gcagaagttc cgttaagtga agaagcttat ttgcatcgtt
    23461 cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat tacactcgta caagataata
    23521 atctaagaga tttaaaacgg cttttacgtc catcaaatat aacacttgaa gagatttttt
    23581 tatacggagg taagctgcaa actgagcagc caaccaaaga agatgcagct cctgcagtaa
    23641 ataaaaagta tgcgaaaact ttttctggaa aggagcagaa cccagaatat aacaaagaaa
    23701 aaataagaaa aaaagttaaa aataaatcaa aaaaagagag aaataaagga gcaaggcgca
    23761 aataagcgcc tttttatgct atgattatga aaacgggaac aaaattggtt gccagaggag
    23821 gggttatgtg aaagaacgtc aagaagagat cttgcagcta ttgacagaaa accaacaagg
    23881 attgacagca agtgatgtag ctgagcgctt aactatcgat cgaagcaatg caagccgata
    23941 tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc gctggccgac cagtcgttta
    24001 ctctttgcca acggaaaagt caaaatctga tgaagtgcat gtcgactctt ctacgcaagt
    24061 gacttttgaa acactcgttg gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa
    24121 agctgcgatt ctttatccac cgcgggggtt gcatacgatt atttttggag aaactggtac
    24181 agggaagtcg atgtttgctg aatgtatgta tcattttgca atcgattcag agatgctttc
    24241 tgcagatgcg ccatttgttt ctttcaattg tgcggattat gatcaaaatc cgcaactgct
    24301 ctttggacac atctttggga tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc
    24361 cggtctaata gccaaagcag acggcggtat attgttttta gatgagatcc accgtttacc
    24421 gccggaaggg caggaaatgc tgtttacttt cattgataaa ggtatttacc gtccgcttgg
    24481 agaaagtgca caagtacatg aagcatctgt tcagatcatt ggggcgacga ctgaatcttc
    24541 agaaagtttc ttatctacgt tcaatcgacg gattcctatg gcaatcacct tgccgaatct
    24601 tgcttcacgt tcactggatg agcgttatga aatcatctct ctttttatca aacaagaagc
    24661 gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg attcttgcct tcatgcttta
    24721 tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc
    24781 ttttttacat taccgtactc atcatgagaa aaagctaatc attcgaaaag aagaactttc
    24841 ccttcaagta caaaaaggat tactgaaaat caaggaaata ccggaacgtc tcgaccgatt
    24901 catggacagc aaaagtcagt atttgacatt tgaaccagga tttgctgatg ttgtctggtc
    24961 acaagatcca gaacgaaaca tgcaggtcta taacgatatt gaggaaaaag tcacttctct
    25021 ttccgaaacc ggtgtggaga acattgatct agagaccctc atttcaaaag atgtggatgc
    25081 ttatttccag acatatgtca aagaattgac taaaagttcg attcccaaag atcttttgcc
    25141 agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg
    25201 tatttataat gaaaaagctc gttttgcttt tgctttgcat ctgcaaagta cattggatcg
    25261 tgtgagagaa ggtcatatga tcgtccaccc agatttgaat aacgtacgga aaaattttaa
    25321 gagggaattc caggtagcaa tcgatctttc aacgatcatt gaagaagaag aacaagttga
    25381 gattcctttt gatgaaatcg gctttattag tatgtttctt tctatcaatc taggagaaag
    25441 tgagcctgtt aaagaaaaca aagtagatgt tctcatattg atgcacggca gacagactgc
    25501 ttcaagcatg ttggagatgg cacaagaact attaggaaca aaagtaggga aagcttttaa
    25561 tatgccactg gttatggaag ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa
    25621 ctatcaagag catttgacga atggcctact cttgttaaca gatatgggat cattaaacac
    25681 cttcagtgat ttgatttatg aagagactgg cattcggacg aaagctatca cgatgacaag
    25741 taccatgatt gtgatagaag cgatacggat ggcaagtgtt ggaagaagct tagaagatat
    25801 ttatcaaaat atccagcttt cctttgaatc gatcgtacgt gatcagttca aagcggaaaa
    25861 gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt
    25921 agctgccaaa ttatatcagc gtattatgcc agtgattaat caaaataagg tagaactgat
    25981 ccagatgcag tttttagaac gtgaatcatt caaaaaacac atcgatgcct tattggaaga
    26041 atatgagatc aaagcaattg cgggaacagt tgatgttgat tatcaaaata tcccattttt
    26101 ttcagcctat gacgtttttg atgatgaaaa actaaatatt ttaaagcgta tagctgatga
    26161 tgagatcccg acagctaaaa ttgtcgattc acttgctgga tcattaagcg atgtcttttc
    26221 tattgatgaa ctgatccgtt cattgcaaaa gacagttcag cagatccaga ccgatttgtc
    26281 gattatcgtg gaacctggag tagatactgg aatcgttatc catttagcct tcatgttaga
    26341 cggattgatc aaacaagaaa gccatcggca atttgagaaa ctgtctattt tctcaaaaat
    26401 ttatcgtttg gaaatggata tcgttcgggc aaacttgatg gcactagaac gaaaatatca
    26461 ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa atgtttcttg aaaataaaat
    26521 aaaaaaatga acacactaat catactgttt acacactaaa catctgtgtg cgcactataa
    26581 aaaagagcta aaaagcatga aaacgctatt tcagctcttt tttttattgg catgcttctt
    26641 gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca
    26701 tgttagtttg ttcagcaggg atgagtacga gtttattggt cacaaaaatg caaaaagcag
    26761 cagaagaaaa agggatggaa gccgatattt ttgcggtctc tgcctcagat gcagataata
    26821 atttagaatc taagaatgtc gatgtattat tattaggtcc tcaagtacgt ttcatgaaag
    26881 ctcaatttga acaaaaactt gcgcctaaag gtattccatt agatgtcatc aatatgcaag
    26941 actatggcat gatgaacgga gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa
    27001 tcaagtatgc agttgggggt ataacaacgt ggaagatcaa caaaatctag aagccatcat
    27061 ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc
    27121 taaaatcggt gattttgagt tagcggatag gaaaatcgct gatgcagaag aatcacttat
    27181 acaagcgcat catgcacaaa cagacatgtt gacacaagaa gcacaaggaa atcatatgca
    27241 agtgacatta ttgactgttc acagtcaaga tcacttaatg acatctattg cgtttactga
    27301 tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac agcgaataaa tacaacttct
    27361 tttttgaacg gaagaagaaa aatttttgaa cggatttttt attttgttat aaggagaact
    27421 ctctttatat aaaaaactta taggaggaag taaaaaatga acggactatc aagttggatg
    27481 gagaaataca tcctgccggt tgccgcaaaa atcggagcgc aaaaacattt agttgctttg
    27541 cgtgatgctt ttatcggcat actgcccgca acaatggcgg gatcagtcgc tgtaatgatc
    27601 aatgcaatta ttcgtgattt accgcaacag tttaacagta cctatgctga cagtttagca
    27661 gcagacaaag gaatctttgc cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg
    27721 aacggtacac tagcaatcgc cggattgatc tttgcctttt cttgggggta taatcttgcc
    27781 cgagcttatg gagtaaatga tctagctggg gggatcgttg gtttagctac cttgattcaa
    27841 ggtatttcct ttggtccagc attatcagca gcattagatg tgaatgttcc ttcaaatgtc
    27901 gcagatgcaa tcaatagttc tgagattggt gcaacgattg cagatggcac actgtcagtt
    27961 ggattatggg gttggctaaa actagatcat ttgaatggaa acgcttattt tacagttatg
    28021 atcatgggag ctatttcagt cattattttt tgtaaattga tgcttgccaa cattacgatc
    28081 aaaatgcctg attcagtacc accagcagta tctaaagcat ttgcagccat tttaccagcg
    28141 acgatcgctt tatacgtcat tgctattatc aattatatcg taggtcgtgt agctgatggg
    28201 caactgatca ttgatttggt gcaaaaatat attgcagaac cattcctagg attatctcaa
    28261 gggttgggag cagtattgat cgtcacagta tttgttcaag tattctggtt ctttggtatt
    28321 catggatcaa atgtattggc accagtgcta gagggaatct ggggacaagc acaactaatc
    28381 aatattgata ttttccaaaa aggttataac ggaaaaactg gtacaccagc cgtgttagat
    28441 gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg
    28501 tttggcggat caggcggaac gatcgtctta ctgatagcta tcttgatttt ctctaaacgt
    28561 gcagattatc taacagtagc taaattatcc attgggcctg gtatcttcaa tatcaacgaa
    28621 ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca tgtttatccc attcttgatt
    28681 gctccagtag tagcaacagc aattggatgg acagctactt atcttggcct agtcgctccg
    28741 gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat tgtcattctt agcaacagga
    28801 gcggattggc gggcaccgat cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg
    28861 gcaccatttg tcatctctgc aaacaaaatg gaccctgccg caggcgaaca acaataagat
    28921 gaacgtgagg agcgggacgc tttaagtttc gttcctcata atgaattttc gaggaggaac
    28981 tgaaatgatt acaattgcca gtgcaatgca acaagatgaa atcaaagaat tgttagaagg
    29041 atacaatgaa gaaggaatga cttttacatt taaagaaaaa caaggaatca agctgttctt
    29101 tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc
    29161 acagccttgg ggaacagtcc tttattttca atcaacagct gtttagaaaa tggtttaaaa
    29221 agataggatg gttgagatga taaaaaacgg tttattttta tgtagtattg gttctttgat
    29281 cgtgttgtat agtttgaatc cacattcgca aaggtatgat ttgtatagta tggtcacagg
    29341 tctttttcta acaggattag gaatctattt cttcatgaag gggaaaaaaa gagaagaagc
    29401 aaaggagaaa aataactaat ggaaatcacg aaaaaattaa atattccagc atcgtttttt
    29461 tacgataaag ttatggactc tgtccttttc gatgtacgta aagcgaccgg caaatcagta
    29521 actagaaaac agctgaaaaa tttggagtac gtcaaacaat tctcgcaaaa taaccgggct
    29581 cgcataaaaa tcgaagaagt cattgaaaac cagtcttata agtttcgtac atcgacaacg
    29641 aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt
    29701 cattacacag aaaaaatgga gtcttatggc atgcttcaaa agttgaatga tatgctttta
    29761 ggaacaatct tgatgtattt taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa
    29821 gcaacgtatt aaatcaatga aacatggtac tatttagtta ttgaaaaatg aagtaagcta
    29881 gaaggaattt ttcataggaa gatagatctt tcctgcgaat cctcactagc ttttttgtag
    29941 gaaggggcag caaaatgaca aacaaagtaa gagagctagg tgtatcttcg attgaggaaa
    30001 tgtttgattt agccacttat gcatttaatg gggcagatac acctgaaagg cgagaacgtt
    30061 ttcgtacact tgcggagaat tcttggaatt acggattttt tgatgaggag gatcggctaa
    30121 caagtcaagt catggcgacc ccttttccag tgaattttta cggacaagag tacctaatgg
    30181 ccggcattgg ctatgttgct tcttatccag aagcaagggg acaaggcggg atcaatcaga
    30241 tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt aagcttatct tatctggcgc
    30301 cattctccta tcccttctat cggcgctatg gctatgaaca atcatttgat aaaatcagtt
    30361 atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta
    30421 tggattttga agatgcaaaa gctgcaatca gaagaattta tgaacagatg ccggaaaacc
    30481 aacgagctgg tctgaagcgc gaagactggt ggtatacgta taaattcaag caaaaaggca
    30541 attaccagtt tgctttgcat tttgatgata atgaggaagc aacgggatat atcgtttatc
    30601 agcttgcaac gtcttccttt attattgcgg agtggggatt tttggatcat gatgcttttt
    30661 gcagccttgt tcgcttcgta agttctcaca atggtgcttt tgaaacattt gaatattcat
    30721 gcggctacgg cggtaatgat caaaattatc tattagaaaa tccatttgct tctgtaatga
    30781 tcacacctta tatgatggcg aggatcgtag atatttcttt gtttttggaa aattatccat
    30841 ttaaaaagag acaagctgca tttgccctac aaatagaaga agatcattat gcgaaatgga
    30901 atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa tcatctctca aaagtagaac
    30961 aaacaaaact cccaatcgtt cgaggaagta tccaacatat cacgcagcta ttattagggt
    31021 atcgtaaagt ggaagagttg gtattccaag aaagaataca ggtgacaaaa gaatatgaag
    31081 aaacgttaag ctcaatcttg ccacagcaac agccaattct taatgactat ttttgataaa
    31141 aaacgggtga tgacaatatt tttgtcacca cccttttgtt tttcatttgg catttgaagg
    31201 aaaatgttaa agtaaatcct cttttttcgt aaatttgaat tttttgtttt cttttgtttt
    31261 agggttttct tttttgtggt cttggattgc ttctttcgta atcttttccc gatttttttt
    31321 agatgaatct aagcgttctg cgtatacttt ttcttttggc ttatcattca ttggaaagga
    31381 cctccctaca ttatttcatt ctacctctag tttatcatct gttaaggact agtggaaacg
    31441 ttttgcctaa cttataagct gattttttta gcagataagc ttgcaagtaa aaaacaaaca
    31501 agctacaatt gatttattga aattgttcct gtagctcagt aggatagagc aatcgcctcc
    31561 taagcgatag gtcgctggtt caattccagt caggaacgta taaataaccc ctgtatcctt
    31621 tgcggtacag gggtttgttt tgtatttgtg ccaaaaatgt gccaaactag ttatcatttt
    31681 tatataaact atcgaacaca gtattgattt tttctttatc ctgatcttca agctctcgaa
    31741 caatatgaag ataagtagac at
//
LOCUS       DAZKOB010000019        31012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000019 DAZKOB010000000
VERSION     DAZKOB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.019"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..331)
                     /locus_tag="ACG8M6_000934"
     CDS             complement(<1..331)
                     /locus_tag="ACG8M6_000934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456622.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(345..602)
                     /locus_tag="ACG8M6_000935"
     CDS             complement(345..602)
                     /locus_tag="ACG8M6_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456623.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(797..1339)
                     /locus_tag="ACG8M6_000936"
     CDS             complement(797..1339)
                     /locus_tag="ACG8M6_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0456624.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1360..2451)
                     /locus_tag="ACG8M6_000937"
     CDS             complement(1360..2451)
                     /locus_tag="ACG8M6_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0456625.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2459..4669)
                     /locus_tag="ACG8M6_000938"
     CDS             complement(2459..4669)
                     /locus_tag="ACG8M6_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0456626.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4685..7213)
                     /locus_tag="ACG8M6_000939"
     CDS             complement(4685..7213)
                     /locus_tag="ACG8M6_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0456627.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7265..7675)
                     /locus_tag="ACG8M6_000940"
     CDS             complement(7265..7675)
                     /locus_tag="ACG8M6_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0456628.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7688..7906)
                     /locus_tag="ACG8M6_000941"
     CDS             complement(7688..7906)
                     /locus_tag="ACG8M6_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0456629.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7903..8895)
                     /locus_tag="ACG8M6_000942"
     CDS             complement(7903..8895)
                     /locus_tag="ACG8M6_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0456630.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8974..9885)
                     /locus_tag="ACG8M6_000943"
     CDS             complement(8974..9885)
                     /locus_tag="ACG8M6_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0456631.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9904..10248)
                     /locus_tag="ACG8M6_000944"
     CDS             complement(9904..10248)
                     /locus_tag="ACG8M6_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456632.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10268..10546)
                     /locus_tag="ACG8M6_000945"
     CDS             complement(10268..10546)
                     /locus_tag="ACG8M6_000945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456633.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(10905..11405)
                     /locus_tag="ACG8M6_000946"
     CDS             complement(10905..11405)
                     /locus_tag="ACG8M6_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456634.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11406..11951)
                     /locus_tag="ACG8M6_000947"
     CDS             complement(11406..11951)
                     /locus_tag="ACG8M6_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456635.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11948..13204)
                     /locus_tag="ACG8M6_000948"
     CDS             complement(11948..13204)
                     /locus_tag="ACG8M6_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0456636.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13565..13876)
                     /locus_tag="ACG8M6_000949"
     CDS             complement(13565..13876)
                     /locus_tag="ACG8M6_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456637.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13899..15374)
                     /locus_tag="ACG8M6_000950"
     CDS             complement(13899..15374)
                     /locus_tag="ACG8M6_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0456638.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15823..16275)
                     /locus_tag="ACG8M6_000951"
     CDS             complement(15823..16275)
                     /locus_tag="ACG8M6_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456639.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16277..16642)
                     /locus_tag="ACG8M6_000952"
     CDS             complement(16277..16642)
                     /locus_tag="ACG8M6_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456640.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16741..21024)
                     /locus_tag="ACG8M6_000953"
     CDS             complement(16741..21024)
                     /locus_tag="ACG8M6_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0456641.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21171..21410)
                     /locus_tag="ACG8M6_000954"
     CDS             complement(21171..21410)
                     /locus_tag="ACG8M6_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456642.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21500..21742)
                     /locus_tag="ACG8M6_000955"
                     /pseudo
     CDS             complement(21500..21742)
                     /locus_tag="ACG8M6_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21855..22106)
                     /locus_tag="ACG8M6_000956"
     CDS             complement(21855..22106)
                     /locus_tag="ACG8M6_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456643.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            22269..23228
                     /locus_tag="ACG8M6_000957"
     CDS             22269..23228
                     /locus_tag="ACG8M6_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0456644.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            23398..24078
                     /locus_tag="ACG8M6_000958"
     CDS             23398..24078
                     /locus_tag="ACG8M6_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0456645.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            24168..25202
                     /locus_tag="ACG8M6_000959"
     CDS             24168..25202
                     /locus_tag="ACG8M6_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0456646.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            25216..25551
                     /locus_tag="ACG8M6_000960"
     CDS             25216..25551
                     /locus_tag="ACG8M6_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456647.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            25535..28015
                     /locus_tag="ACG8M6_000961"
     CDS             25535..28015
                     /locus_tag="ACG8M6_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456648.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            28071..29630
                     /locus_tag="ACG8M6_000962"
     CDS             28071..29630
                     /locus_tag="ACG8M6_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0456649.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            29627..30991
                     /locus_tag="ACG8M6_000963"
     CDS             29627..30991
                     /locus_tag="ACG8M6_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0456650.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
BASE COUNT         9440 a         6415 c         5061 g        10096 t
ORIGIN      
        1 atgaattcat tccccaaagg ccttttaaga ttgatacagc agtccgttca cttcttttag
       61 acttgtcata caagtctttt aaatgatccc atatcagact agtttgtcta tctagcttca
      121 atccattaaa cgctaatgaa cggttcattt tttctattac tttagataaa gctacagaaa
      181 tttttatacc ttcacaacgc gcatcttgga tttcaataat agtatcatca attagttttt
      241 gaatttcttg cctatcaaca tattcaatct tagaaagaga atcttttaaa gctactaatg
      301 attcaacgat ttcttttttc tcttcagaca ttttcttcct ccttctagtg acccgtaaag
      361 ccatgtagag cagagtccat tgaaccagta attacactat ctacaacgcc attccaattt
      421 actctacatt ttccattttt cagatttgtc ggtccatacg gatagccttt ttgacattca
      481 atgttcggat tataccagat aggattatac cattcagccg cagaagcaga ttcagtcgat
      541 acaaacaatc ccacaccaac aaatccaata cacatcacac tatagccaat tagttttttc
      601 atctcgtatc catctcctaa aaaatagaat agttcttata gttattcata agacatattt
      661 tttccgtttt tgttttatcc tttctgtcct ataacaactt ttttcaagta aagaatgaca
      721 tagtttttct taaaaaaaca gtctgttttt ttctatttct tcggtgtata ataaatatga
      781 gtgttcattt tttattttaa tacgcactcc gtacataata aatcttgatc gcatcaatct
      841 tttgagcttc ttgatctact ttcaattgaa gtaatacaaa ttgagtaacc ttattcattg
      901 tttcttctcc aaataacaaa tattgatccg gattctctat atcttgtgtc acctggtaaa
      961 gtttcccagt tgttttcatt tgtacatgcg ggtctacatc aatactattg tctttaatac
     1021 aacgatctgt catatagtcc ctcacagctt gattgcgttt ataaacatct gtgaaattac
     1081 tccattttct agcaaaatct tttaagaaat tttttgtcat ttcatctatt tgcatttgtt
     1141 gcgctgtctc gtcttctaat gaaccaattt gactgttact ttcagtactt tgaagagttc
     1201 cacttgtttc ttttactgct tcgattgttt cttctgtcgt tctcaattgt gttttttgtt
     1261 tttcagaaga aaaccaatga caaccagtaa gacctatcaa acataaccaa ctaaatacta
     1321 aaattatccc ttttttcacg tttctagtca ctgcctttct tatttcacct tacgaaccag
     1381 actacgaatt tttgtttgat tgtacgtgcg ttgataccga gccataatcc gtccttgctc
     1441 ggcattctgt tccacactag agaaatgttg tccgccatat gaaacatctg ttataattcc
     1501 ggtgtgacca tagattccag gagacaatgc accacctgct tgccaattca ccacatctcc
     1561 tgcttgtaaa tcacttggtt ttgggttcat gattacttgc caaccatagg ttgtccagtt
     1621 ataatcttcc cctattaaca tcgcaaacat gtgtccagaa cccattaact gtgggccacc
     1681 taattgagat acataccatg ccgtaccacc ataacattcc ccattattta cggatcgtcc
     1741 aatgatccga tccaaaacct tcgcattgcc tttacttttt cctgcaataa gcgaagtttt
     1801 ttttgttggt aggctattcc aatgcttgat cgcaaaagta tcatattccg ttaagttata
     1861 ggacgttatg atactcattg ctcctcgata atacggactc ccttcggtaa tacctgctgc
     1921 cgcatagcct ccacgaccta gaatcaataa acaacttcca ggatctttat tgcccacaca
     1981 ttgattaaag ttgctttggc tcgccaccat aaagccataa tagtcataac aagcttttaa
     2041 gttcggaaac ttcatataga aaccaccttc agaaccacca attccacgtg cagaaccttg
     2101 tgggtaggga caaccaggaa accaggtaat cccaaaaaag ttcgtatcgt ttcttccaga
     2161 ttgtgactgt ccataagctg attcatagcc taattgagaa agaatcacac tcggtaataa
     2221 ctgatacttt acagctgcac caactgctgc ttgcgcatgt tcatctgtga ttggaccacg
     2281 tcctttaatc tctgaaaaaa tcggtaatcc ttcggtatat tccccattaa aagcgacagc
     2341 tagacctgta gcttctcctc catgctcaga aagttctcct gaaaaaaaga atggcaatag
     2401 aaaaaaggga agaataaaga aaagaagaca acctagacac cctttcttca tttcttcctc
     2461 atcctttctt cgataatggt ttttggactg ttcgtccttt tggtaaagta aaatcagttc
     2521 ctggttttgc gtccaaaatc ggcagtgttt taggatcctt ttctattttt tccgattgct
     2581 ctgttggctt atctaaagaa gaaaatgcag gttgtaacga ttttggtgcg gaaatcgacg
     2641 gtttactttc agacgaacgt tgccccggtt gttgcacaga acgatttcct gatagagtgg
     2701 ctaccgacga attaggaaca gagtattggt tcaataactg ctgcattgct ggataactcc
     2761 catttttcgc ctgatttttc acttggcgaa tctgttgtga aagagaacca gcagctgtta
     2821 attgtggcac gggtactttt ccagaaggca acggaatcga ttgaccaaca aaagcgatac
     2881 ctttatttaa caatggtttt ctttgattat tcccttgttg cttactcaat tgtgccaatg
     2941 attgtacctg ttgttttggt cttactgaat tcatggacga atttggaatc aaaccatttg
     3001 aaagaccaga attccactct gttttcgatt tggtagaaga catttcaggc tctaccccat
     3061 gataacgaac atgacgacca ctataaggca aggcttgatt tgaacgccca cctagttcaa
     3121 agggattaac cacttgcctc ccttttaccg attcttgtgg tgtacgtttt tgttgaaact
     3181 tttgaaatcc atgtatcgct gcagaagcga gtggattcgt tgctcgatac acattccctg
     3241 ctaatcctgc acctttgatt gccaaatttt gtccagcagt tgatagttga ccaggcagtt
     3301 gtttcatttg agacaaagaa tgacttgcta tgtttttcgt gttgtttata gtttgatctg
     3361 cagctcctaa ccctagtttc tttaataaat ctttccgttt tacaatgagt aaaattgata
     3421 ggagtgcttt gacaaacata tttcctaaaa aaccaaaaac agtattcatt ggaattaaat
     3481 cgtctactat gttatagatc aaaaatgcca ccaaaataat cacactgtaa aaagattttt
     3541 gaaacatcaa gcccaaaaag gactttaagg tattcatgag catataatca aatactggaa
     3601 caaaagaagc gagtaatgaa aaaggaatca caagtaataa acacaacacc aacatttgaa
     3661 agataaaacg aatcatgcca atcactacaa gtggtaatcc aatacttaga ttaagcaatg
     3721 gcaccataga accaacgact gctttataaa cagctttagt tggttgaata tagacacgat
     3781 aagccttctt tttccctcta tcttctcctg cagcagtggt cacttttgtc tcaatatcag
     3841 ataattcttc acttgacgct tccttggcta gaaattcttg tggttcaatc tctgcttgat
     3901 acaattcttc taaatttgtt gtcccataat ttaacaatat atacggatca acaacagccg
     3961 ctttataata aagttggcgc actttttgaa tggcttcctt ttgtgtcgtt ggcactaaaa
     4021 caccattgac atcttttgtc gccaattcat ctagactatt tgtagcagaa aaaataaagc
     4081 cttctacctc cacggacaaa tcatttaacc ctttggtaaa agcttctcct ttactaggta
     4141 cgttactacc atagccaaac caaaccattg aaaaaatgac aacgaagaga aatcgaataa
     4201 acaccatttt tgcgtaatgt ttccctctaa atatacgaag agtaaataga tataacacat
     4261 acaagacgac aatccccgca ccaataggat cgtatatttt tgaataaata gtatccgaaa
     4321 aagtgaaaaa ctggtgaatt aactcgttca ttgcagctcc ttcatagagc ttctcaatga
     4381 caaaatcaag gccttgccaa atcaatttac ttaaactaaa gaacatattc gtcatcattt
     4441 ttagaaaatc tgtgccattt gccccaaata acattttgcc taatgaaaat tcactatcag
     4501 ataaataaga agaaaaggta tcaatatgaa atttcccaat ctcatagatc gattgatcca
     4561 cctgcttttc tgccgcatac accaatccac caacaaaaag cagtccgaat ccaatcgtta
     4621 aaaaaaacaa aacgcaccat ttcttttcct tatgctttat ataagccatt gtttcacctc
     4681 atttctattg catgattaac gtatcctgtt cttctacttg agagtgactt gttttttccg
     4741 ttgtttgtaa tgcagcggtc atttcttcaa acaaactgtg tacattgagt tttcctacac
     4801 gtccataggg atctaacatc caacactgtc ctttaatcat gtggctcatt tcagctaagt
     4861 tcgcttcatt tacttccaaa cgtaaatgat tcaaaatctt ttctcgctca ctgtcttcat
     4921 caaaagcaaa gacgacacca aaattccctt tttctttatc tgaacctaag tcaccaataa
     4981 attgtgtatc taacactaat tgattcatct gggaacgccc cacacgtgcc attgctgcaa
     5041 taatactacg tcctgcgttc gaattattaa atacccatgc ttctgtaaaa taaatcgctg
     5101 ttttcttact actatctcgt tttccaaaca tttcacaaaa acgacctaaa ctaatcatca
     5161 aacacaaaga ctttcgatcc gcatctgtat atagttctgg tcggacattg tcatttggta
     5221 atttcaaatc ttggatctct aaaatcgttg ttttggcatt aaagtctaat cccgggtttt
     5281 ctccataaga aaatcctaaa cgtaaaatcg aatcttctac cgtcaagcgt aaaaactcac
     5341 catattctcg aacctctttt tgttccattt gttctaaata gtcgaccaca tgcatcaagc
     5401 caacttgttg tccggcttct ttttgaacta aaacttcttg gattcccttt aataccgccc
     5461 cttttaaaag gtgattggta cctaacggac ttaactcatc aatcatatcc tgggctactt
     5521 gtttcgcatc tactcgggat aaacaaacaa ttggatctaa tacaccataa ttacggggat
     5581 ttgtagcatc taacgtgaca taactcattt tttgcaaatg ttctacaaac aaagggtaat
     5641 gtttctgata atacgggtct tgaatgagtt tcgtaaacca tttgcgcttt tctcctttgg
     5701 gatccacaaa taaactttgt acattcataa acgaactata caaaaagagc aagccaacta
     5761 aaaaggtctt ccctttaccg gtttttccag ttactgctac atgaggcgca tcaaacaacg
     5821 cacctttgat tccttccgca atggctagaa gattcaaaaa gacaaaacga tttgacgcat
     5881 aaattaattc ttctaatggt gttttttctc ctcgagaacc accatcccct aatgtatctt
     5941 gccgaccaat ataccagccg gattccatac ctaattctga tactgctgaa aacaaccatt
     6001 cgccaactgc ttctacagtt gttcggtgct tccaatgtaa ctgccgtccc aatggttgcc
     6061 ctaataaaca tttatagaac aattctcgtt gatcattttt tgcacggtgg atatggataa
     6121 tcgtatgaag ttgttgaata accgttgcac tattttgacg aacttcttca accgtttgac
     6181 cgtatacaat aaaacatgcg atccaatcga cgacaggttg cccattatct actcgttgct
     6241 ttaaatgttt tactaatcct cgaacagtta ataagcgttc actaggttct tcatcatttt
     6301 gaaccaattc cttatgctct gttttgaacc gttccgatag ccaattcagt tttaaaccaa
     6361 tacctccctg tcctttcaag cgtggatagt gaattttcat tcgcaattct gctgggaaag
     6421 aaagatcctg agcaaattga aatagatcac aatttctcat attttcaggt gtttccccaa
     6481 taggcaacaa tgctaaataa ctactcccta actcatcttc taattgaaga aggcctttat
     6541 gttccttcgg tatacgtaca tacgttcctt gattgtcttt cagtacttcc caattatttt
     6601 gttcttctac tttgtgagcc attcctcgaa tatattgcag acgattgaga tacaacagct
     6661 ctttttttac taagggacga atagaaaagc tataaagtaa ttgtgtcact tcttttacat
     6721 aaggatcact ttcagaaaac aaagtttcca aatcatctac ttctaatcca aataagcgtg
     6781 ctaaacttcc tgctaaatct aaccagatta gatttattac atcatgaaaa ctctccaccg
     6841 ttgctttttc tttgactttg attcctaaaa taaatgaagg acgatagact tgaccttctc
     6901 gttccaacaa tgtcactgtt tcttttgcat aatacgcacc aattgcttga ctatcgggag
     6961 aaaatccctt tcctaatgtg tcaaacttcg tccgtaaatt cattgttcgt ggatacatca
     7021 ccaactccac atcttcataa tctgctaaaa gcaacagtaa acgagccatc atttctttta
     7081 tttcctgtgt cttttttggt gtgttttttg aaattgttcg tggaaaaaca tgatagtagg
     7141 cccagataca atcctctcca tttaacaaac aatttttctt gaatgctttt actggttctt
     7201 ttacaagcac caatgaattt cacctcccaa taaaaaagga acaaccagtt gattgtccct
     7261 ttttctaatc ctctaaataa acatacttat tcacaaaaag taattcttcc aatgcattgt
     7321 ttgatacctt gtcccagtta taatacacac aatcaccatt catcatacga atcatataac
     7381 aaaaccaatc ccataaataa aaatgcactt tttttccatc aggttttagt tttgccagtg
     7441 ctaacacaca taaatagggg agtcctatat aaaaaacaac tcctgctggt ggaaaaaaag
     7501 aaatgaccca accaataaat gaacgtaaaa ataaatattc aaagattacg accaacaaaa
     7561 aagttgctgc attgactaat gcaactggaa atggaagacg ccaattgctt gtaatctgtt
     7621 gtacgcgata aggttccgca taaacaatcg aataatcata aggttcccaa tccacttcac
     7681 tatcctccta atccataaat aatttttcaa tgattttacc aaacgcatca aatacacgtg
     7741 ttggatcatt taagaagaaa taaactgcgg cacctgctgc gatagatatt aatgcatttc
     7801 ctacttttga tttcataaaa tttcgcaatc ccagataaac aaccaccatt attaaaaaat
     7861 agccaccttg cgttaatata aaatctacaa ttcccttcag tgtcatttcc gttcttctcc
     7921 tttcctatcc aaccaattat gtgttaattt ttcaacataa tactgatttg tttttttcgt
     7981 aatgacaatc gtcatttgtt ctttttgtaa aagtgacgta tcaccatctc gaaagacgac
     8041 cgttgctttt acaataaaag gttttttcgt ttctttataa cgcaatactt tcatctctac
     8101 ttgatcaact ttagctgttt gattgagtcc ttcaggttct ttcatcatgt atgacatatc
     8161 ttttgcattg tttttcgcat acttatctaa gaaatcttcc acaaatcctt caacatcttt
     8221 cgcttccgac gcatcaaccg tttcgtataa tgtgtcatca taagtaatca ttcgttgttt
     8281 ttctgtattc tttggtagtg ccgtaaaata aggataagca ataatacgac agcccttgtt
     8341 tgaaatagaa aagggaagat tgagtacttg tgtgattgtc acacttgttt ctttcttact
     8401 ctctggattc atcgagcgaa tatcataagt tactttataa ctagcagttg gaatggtttc
     8461 aatttctttt aatccaataa acgaagcagc agttaatttt cgaacacctg aaacacttcc
     8521 tggttcttga ataaagtttt gtgcataaaa ctcttttttt aatgtttctt gacgttgttg
     8581 ttcttgttca tgattatcga atgtattcat atacaaagca acaaaacgat tcataaagga
     8641 ttcaatttca ggtgtgtacg ttaattctga ccgactttgt acttctttct tttgtaattg
     8701 ttggatttca tctttcaaaa acgacacttg aaccttcgtt ttcatggatt gaaaaaatgc
     8761 caagccccct gaaaaacaaa gaaataaaac caaacaccaa atccatttca ctgctttttt
     8821 tgaatggatt ttcttttgct tcggtacttt ctctgtataa actttttctt tccgccgtag
     8881 ttgtcgtttt ttcataagct actccttcta gtatgttttt actagtacgt acgattgaca
     8941 acgataagct tgtttttctg aaaaatagtc cattcaatta ccctcatttt ctattccttt
     9001 tctctttctt ttatatccaa caaatccagc aatacttgtc aacactcctc ctagaacaga
     9061 aagtgcaaca tgtatttctt caccagtctt tggtaagact tttgtttttc catctgctgt
     9121 tttgatttca atattgccac ttggaagttt ctttacagga atcgacaaat cctttaccaa
     9181 attcccttca ctatcttgga ttaatggaat accatctttt acaccaacaa ttgtttgatc
     9241 acctgctact tttaatgggg tggttggtgt tggaatatgg gtcaattcct ctacattact
     9301 agtgacaatc ggtacactca ttccttgaga agtattcgtc gtaatttcac cgcttggggt
     9361 taccgatact gtgacttctt ttgctggaac aataggttta ttttcttttg gaattgcttc
     9421 tgatttttca acaggttgtt tcggttcttc tttttgttct tcactggtag acgacatcgt
     9481 tggctgttcc gtacttgacg aatcagtatt cttttcagaa gaactggttg ttcctgattg
     9541 ttccggagtt gtgactatac ttgaatccgt tgttgttgaa ctatgcacac cttcttcttc
     9601 gatcgaatca gtcggtaaag ttgattcagg aaactctggt tctggtgtca ctggctcttt
     9661 ttcaggtttc tctgactgca attgttcagg ttctgctggt ttttccgttg ttcctccatt
     9721 tagctctgtc tcaacatctg gtaatccttc ccctggcaat gtttctacaa tttctgactt
     9781 aggtgtttct acagtttcat ctgcataaac caatgaactc cctaattgaa acgaaaataa
     9841 tgaaattcct agtgttgtta cgactgctaa tgttgttttt ttcatgatta ttcctccaat
     9901 tttttatgca attcgtgttt tgttcataga cgccgcacta atattatctg aacgataaaa
     9961 gaactcccgt ccgttttctt ctaacccaac atatggatta tttttgaata attgaatcaa
    10021 tagttcactg gtgttgtatt ttggatagtt tgcaaaaaaa taatactgtg cttgtcgccc
    10081 atccgaaaat tcaacaagta attcataacg ataccgcttc aatccttttc gttgttttaa
    10141 aggatctcta taccaacttg tccatcgttc cattcgtgga tagagataga cacgatacac
    10201 atagaacaat gccataccac ataccaccaa aaataataat cgccacataa aaattccccc
    10261 ttttcttcta cctttcttga atcatcgaac attcataccc tctatagaga tttgaaaaat
    10321 ccatcatcaa ttgttcatat aaacgttgtt gcaaatagcg attgtttcga atacataatt
    10381 cttcatagcc atcaatcact tgatcttctt tttcatagca tcttgtagct ctatgacgct
    10441 cagcttgcat gacataacca aactctgcac catatcgttc tactgttaat aactgttgac
    10501 tacgcaataa tgcaatataa caatcacaaa aatgttcaac cgacatttga tttttctcac
    10561 gtttgtgaaa taggtattct ttagaaagca cattattcgt taaacaatct gataaatagc
    10621 gataatctgg cggtcggtta ttttcatctg tgaatttttc ccataataaa ctgattttta
    10681 ttcgttcagc ggctagatag tcttgttcgc gtggataaaa ttcatcaatc gcacaaagtt
    10741 ttttatcacc tgaataaaca aatccatata atgcgaaacg aactcttcct tttcaattgg
    10801 ctctcctaaa aattccagtg acataccatc ttctccttcc taaaaaaaat aaaaaagcag
    10861 aaaccgattt ggcttctact tttctttcct tttatagctc ttttttacgg aagaatccgc
    10921 aaatgacatt ttttcaaaaa atcaatcggc acaagttcac gaaaacttgg agcaattttg
    10981 gatagaatca cttccccatc ttgtaaaata caatcataaa aatcatcagt tacttgatac
    11041 ttacgcttct tacaataatt gataaaatag tagcgatcat tttctatttc cattcgttgt
    11101 tctactaagc gcatacgaac atcatcatag aaataagcca caaatcgccc ttcaagttca
    11161 tgtttttcaa tgggatactc ttctggtaac aatctatttt ttcgtcgttt atacattgct
    11221 ggatacctta gaagaagttc atctaaacgt ctgtcagtat atggtaactg acgaatcaac
    11281 gattctaatc gttctttata cagatagaat aattggcgca agcaatagtg taccgcctct
    11341 ggattttcta atactgttgg atcacgtgac caatacgcac caacataaat cataattttt
    11401 ttcatttaga caatcacacc agcaatacta atgaccgatc cacaataggt agaacaagtg
    11461 gcatagcgac caatttcctc taattctatc tcaatctctt ctaaagaaag atatgtctgt
    11521 tcttctgttg gctggcgact catttcccaa gtgacagtca ccaatgaaat ttcttccata
    11581 agaggttttt cgctgctaag tgctgccaca cagttttcat atatcttcat tttatcgcag
    11641 ccttttttaa ccgctaaaat cgcaggtatt ggcaatgaaa atcgataaaa gcgaatgaat
    11701 tctagccttc ctttccttcg gctaaattcc aactcttcga gttcttgttc gtatccctta
    11761 cgaacttcta ataagcgtgc aatttcttgc cagttctctt tttcctgata acgtccagct
    11821 tttaccgctt gttcaatata aggaactcga aaacgaaaat aagacaaatc aaccatcttt
    11881 tcatttaatg cttcgattgc agcctttaaa tccaacaggc gcagccattc tgttgtcgta
    11941 tatgcattca tgcaattttc cctcctgacg ttcccatcca ttctagatag ttttgttgct
    12001 cgtcatttag ttcggcatta tcaatccctt cttgtacaaa gtctcttcct aaaatacgat
    12061 ctgcttcttg taacaacttt aatgtcggag ctacttgttt caaaaaccag cgttctgaac
    12121 gatctaatga gaatttttct ggctttactg taaaatccaa ttcttccatc gaacgaaata
    12181 attttgccca tcgtttatca acaatccgtg catcactttc cagtctatcc caaacaataa
    12241 gttttgcatt aattaaacca acgccaatct ctcctaaacg tttcggtgaa tcacctaaaa
    12301 taaatgcttc tactgcttgt gttgctttct cattggctaa ccgtaactca tatcgattat
    12361 aaataccata tttcgttaat gcttctaatt ctgaaatttt ttctcggttc gcaatttcaa
    12421 aacgtttctc ataaaaatta aaaaagagag gactctttcg cgatccaaaa tacaaagata
    12481 atccttcact tcgaaaatag ccatcatcta aaaaacaacc gccaatattc tggaatctct
    12541 taaagtccat ttctaataaa cctgcttttt gtttttctag taattcaaag agatcgaaat
    12601 gttcttccgt ttccaatccc caaagttcat ctaacgcaat atcgaatcgg ctacattgca
    12661 cacgtgtata accagcttcc aactttcctt gatttttcca aatggaatat agccatgttc
    12721 gccaatctgt ttgttgttca cgcatgactt cctcatattc ccggcaacct tgtcctgaaa
    12781 attgcacaag tacattttct gttacagttc gttttgaatg ccagaacacc cagatattcc
    12841 cacgaacaga aagacagtca aaaagataca agcccgttgg acgtgtttca aattctgtca
    12901 gatccattcg taatacacgt tgaatcactg ttttgtagtc tgtcccaaaa aaggaaagac
    12961 gcaaataatc aatcattgct cgaagatcta ttccgctttt catatacaat cgaaagcgtt
    13021 ccttgattcg ttgttcaaaa tcctctggga tttttctttt cccttgttcc atctttctaa
    13081 tgtaatcttt tgagcagccc atcaattgcg caaatcttgc ttgtgatagc ggctttttat
    13141 ctccacttat attatgaatt gcacttaact tctttctgta gctttttagc tcaatcggtg
    13201 tcattttctc gctcctttta taaaataaca aaatcgattt tttttaaccc tttagaaaca
    13261 ccgatatatc aactagtccc cctcgttttt tgggaaaagt agtacaactg ctgaccttag
    13321 ataatcactt gatacaaaag ggtttataaa acttttttct atgtgttatg ttatttctgc
    13381 cccccatatt atatatcggg gggctttaca caaagaggcc gtgcgcaagc gcacgacctc
    13441 tttgtgagct gcacacgctg ctagcgagtg cgcattatag gcccatgctt cgcagcctta
    13501 atgcgcaccg ccaggcagag tgcggctacc agactacgtc tagagccctc ccgatagata
    13561 ataactacaa aattaaatga tctaattttg ttatttcact atatttttca ggagaaaaaa
    13621 cgcgataaat tgacaaagtt aaatcctctt ctaaatctac tcgataatga tacaatcgag
    13681 tcaatcgttc tatatctttt ttcgttagca atacttgatt aataactttt cctgtaaaaa
    13741 cacgttcttt gcgcaaaaag cggtaatcag ttacttgttc aatacgattc aaccaatcat
    13801 aaaacgttct ggaagaaaga gaaggattaa tcattcgtat tgctgtagct actctataat
    13861 aacaactctc tgccatttaa cttcacctcc ttagttgatt acaaaatatg cgcatcagaa
    13921 aaacttgttc cttttggatc ttttgctaaa tacatttggc gctctagttg ttcttgtaac
    13981 aactgattat catctcccat cgtttgatct tgactatcct cttttactgt aaaatcatag
    14041 acaatcattt cagtaaattt ctcaaattct ttgataaagt gataatcttt gtaaacaaat
    14101 ggcgcaaaaa actctacagg tacatcaaat cctggaaaag catatccacg accaggttga
    14161 tctttgttgt aaaatggctt atttttaaaa tcttctccaa aagtcattaa ataaccaatg
    14221 ggagacaatc gaccaacaga aatacggaat gttaaattat cccgaacacc acttggaaaa
    14281 tattctgcat ctggtcgttg cattgctaaa ataatgtaca tccctgcttg acgacccttt
    14341 aatactaatg gctgaatcaa tttatcgact ctatttctcg cttccatatc caacgaacca
    14401 taaaaagctg cccattcatc aaaaaagata aaatgcggtg gtaacccata ataagcataa
    14461 tttttccctg gttcatattt aggatctttc atggttttca tataggcata ccgttgattc
    14521 atttctttta atgcatttcg taaacaaata atcatttctt catttatttt gtaaaacaca
    14581 tgtcctttga atacatcgac gtcacctaat gctgctaaat ctgccgcttt tccatcgcaa
    14641 atatctactg tcccaacact taaacaagct tgaattaaag aatacaataa ataggttttc
    14701 ccaccaccga ttgcaccagc aatcaacata tttggtgatt gatcatatac ccaatccaaa
    14761 gttttcatta aatgaatacg tccattttct gctatacatt gatcaatagg aatccgacca
    14821 gaatctgaaa ggaatacata attgattcgg gctttttctc gttcttcacg aattaaatca
    14881 cgcaaaaata cttcagataa caaatgaccc atttttaaaa atcgttcgtg aaaacgccca
    14941 ccatctaaag gaaaagaaac cactaattct tgtttctttt gataaaaata aacttttggt
    15001 aatctcattt tttcccttgt ttgtttctca ctaacttgtt tctttgtata aacaaaccga
    15061 ttgtcaatta acattcgaac aagtaactga cgccaataaa cagaggcaaa atatccttct
    15121 tttagtcgcg accaatgcat caaccctaaa attaacccac taaccagcaa caaatacaac
    15181 acaatactta ttccaggaat aagatacatt tgccaattcg aaaacgtact taaacgtgga
    15241 taaaatactt tccaactaaa ccaacctaac agaatcaaaa atggaaacaa aaagaaacca
    15301 taatagcaaa accataaata acgattgaaa tagcgaattc gccgaccacg atacttgaaa
    15361 aaatgagctt tcaattgtca cattcctcct ttctgttttt acttttcctg tgctaaaaac
    15421 ttttgacaaa aagcaactaa tacaggaata tcacatttag taaaaaaaca taatcgaaaa
    15481 agatttaatc gatttttata agggtgtgca cacatattac atattcgtac tatacgaatc
    15541 caaatcactt cctgatgatc ttgtttccac tggtacggta atgaaaaacg aacaagatca
    15601 cgtaaaaaca tacactgccc aataaccgca caaatatatg catttgtttg tacaaaaatc
    15661 gttggattgt ctccaaaata ctcaatcaat tgttggatat tttgataaca agcacaatct
    15721 ctcaaagtat ttgattggtt ttatctgaat tcaaaatctc cccttccttc tatcttagat
    15781 aaaaagaata ggaagactcc ttcctattct ttcaaaaaaa ccttattttt tctctttttc
    15841 cttagaatca acacttggtt gtttttgatc taaacctttt tcttgtgatt gtttcatatt
    15901 aggattattt gttggagctt ctactttctc taatcctttc gcataaatca ctaaatctga
    15961 atattcacga ttgccatctt gtgctggaat atataccaca tgatattctg gatcaattaa
    16021 tttcacagga tcacgtcgtt taaaaccagt caaatctaca tctgctggaa atttaactcg
    16081 tcgtgcaatc tttggttcat ctggatttcc aaaaactaat tgtgctcgct gtgatcgaat
    16141 ttctcccgta taatcgcctg tttcattatc aaatacacga tcagactctt ctaatgcgcc
    16201 aaagcgtacg gctcctaaac aagtttcatc taatgcttct gtaatgtcat aattatttgg
    16261 aaattttaat cccatcttac aaaacctccc caatttttac accagttaaa aagacaccaa
    16321 catcttgata gccagttgtt cgacgctctg tttgtcccat atcatttgtt tcacgactat
    16381 tcactcgttc tttacgaaaa acaaaataag cttcttcaaa tgaaatacgc acctcttttt
    16441 ctggtaatcg taatctttta tcctcttcag ctgtgagata aacaaaaaaa gcaggctgtt
    16501 cttctcgcga acactctgct tgtaattgaa catatcgccc agacacacca ttaatttcct
    16561 ttggtggatt aggttcccgt gaacccaata aaaatacttg acctaaaaac tctttcttca
    16621 tacgcaatgc tttttctgcc ataaagtttt tcctcctctt aaaaaccaaa ataaattcta
    16681 aatatttcta cattacaatc aaacaagcgg ttaaaaatcc taaccgcttg ttcctcatga
    16741 ctaatctttg gctttacgta ctttataaag tgttatacct aaagcaatga ctaatactac
    16801 taatccaaga accataagta tactacttgt ttgttcacct gtttttggca acattggtac
    16861 aacagtagct ggtttaaccc gttgttctgg tttcactggt tgcggtggtt ttggcgcttc
    16921 cacaatttga accgtttgat ctttgtttgt tagatctaca ttgtgtttcg ccacttcttc
    16981 aaacgtttct ttctcatctt ccgttttcgc ttggagccat tccgcaaaac tcaagccttt
    17041 tcctgcaaga ttgcgaccgt ccactaagaa ttcaaataag aattccatca tttctttatc
    17101 ggccgcaaag actttttccg catgagtgcc ttcaaatggt gtgccgtctg gtgttaagta
    17161 gccttgaaca attaagcgat agatttgacc aatcgcaaga ccgtctactt tgacccattc
    17221 aagcacagtt tgatttggca aggccaagat ctcttttccg ccatttttat tcgtcgcttt
    17281 cgtcgtaatg gttggattac tgattttgat ggtttggtca ttgtcggtaa tatctgcgtg
    17341 agaagccaat aatacgcctt ctctaaacag atcttcaaag accacgactt ctttgcctcg
    17401 caacgcacgt gcgtctagaa taaagtctag attgactgta ccatccgttg tttctgccac
    17461 aaacgttaag gtacttgtga cttcttttcc atctactagc actggttttt ccgttacttt
    17521 gtccattaga acaccgttga ctgtgtatgt ttttccaacg accaaatcgt ggtatttcac
    17581 agcgtctgta atggtgactt tcgttaatgg attaacctct ttcaagccag tgggtttatg
    17641 tgtagccgtt gtacgtacac ttgggttagt tactcgtacc gtttgccctt tgtcgttgat
    17701 gtctttatgg tttgctagtt cttcatcatt tctaaaaaga ctttcaaaca cgacgatttc
    17761 acgaccattt atacctgtca aatcaaaggt aaatggtacg atcaccgttt cattttctgc
    17821 tttggcaata aagccaacac tcgtttctac cgtatttcca tgcacaagca agggttcacc
    17881 ggttgcttta tccattaaga tccctttgac caagtatgct tttcctggaa gaatctcggt
    17941 aatttcaacg gtatcgttta aggtaaacaa tcctgttggg tctgcgactg tcaatccgtc
    18001 ttcaaagaca gccgtggtat gaacggttgg attattcact ttcacacttt gggcttcatc
    18061 gttgatatcc gcatgtgctg caacttcaat gccatcacgg aaaagttttt caaaaacaac
    18121 gatcgtttga cctttcagaa cactagcatt aaccacaaat tcaaggtctt ttgtgccatt
    18181 gactttgtct gcaataaagg ccaaggtact tgtgatttct ttgccatctt gtaaaattgg
    18241 cgcattcgtt gccttgttca tcaatactcc tgaaatggtg tattcttttc cgacaaacaa
    18301 gttggtataa gaaacactgt ctttaatgac gatcgtttct aatggatcaa ccactttttc
    18361 accatcggct ttatttgacg cttgtgtttc tagttttggt ggaatcaagt aattacgtac
    18421 agaaaccact aacgtttccc catcatctgg cgcattttgt ttgaatgcaa ccgtgaatgc
    18481 ttgtttatct gtatttggtt ggaaactttc taatggggca ttttccaaga agtaatagtc
    18541 atcggcaact agacctttca ctaaaatttc cccgttttga tcagtggtat attcaccaat
    18601 tgcttgattt ttaccgttat acaaggtaaa gttcacacct gcgagcggcg ttttcttctt
    18661 gttccattct tcatacttga ataattttac acttttccgt gcttgacggt tcgcaaaatg
    18721 gatcactttg atcggttctg tatcattggt aatcgagaac actaatgggt tttgattcaa
    18781 aacgtaattt acgggggctt tgatttcttc caaacgatag cgatcttttc cataagtaag
    18841 tgtaccgtta gttaggaaaa agcctttttc attggtttta aagatatccg ttgtttctgt
    18901 atcatttgga atctccattg tgacataacg attggctaaa gtatcaaaaa tcttgaattc
    18961 tgtattagca agtggaatca cttttcctgt ttcttgatcc actttcacca ctttaagacg
    19021 ttgttcaatt actttatctt ctaaaacata atggaaagtt tgcccatttt cagaaattgt
    19081 gacttcaagt ggttcaaata gtttataccc ttcttttcct ttggtttcag aaagaatgta
    19141 cgtatcaaag acaacggtat ttttaccaaa agtagcataa ccatcattgt ctgtcacaac
    19201 ggttttcacc actttgccag ttgtttttga cgtcaaagtg atttcaatgc cagctaattt
    19261 tggtttgtta tctggattcg ttgaatcgac taaatcttta ttagcaactt tcactaaatc
    19321 aatggttcct ttgatcactt gatcttttgc ggtcgcattg acagtttttc caggttcaac
    19381 aacaatttca attgcttcat cagaaagcac ataacctttt ggcgctttga tttcttttaa
    19441 aacatatttg ccaggatcaa gatctttaat ttctgcctgt actttgccgt taattttttt
    19501 caaggtaatt tctttaatct ttttcccatc tgcttgatac aaaccataga ccgcattttc
    19561 taaagaagct gcaccttgtg ctttcacacc agtttccgca tcttctttaa acacacgaat
    19621 gtttccttgt tttagaattt gtaaattaat aacagacgca ttaggttctg ttggatgtaa
    19681 cacaccaatt gtttgagctc ctggacgacg ataggctaat ggcgcacctt gaactttaat
    19741 tttattcaat tgaatttttg ctttatccgg tgcctctttt gtgactgtaa tccatacttc
    19801 attgccgtga atagatacat tgacaccttt tggtttttga atgtctttca catatttcaa
    19861 aaaatcattt ttattcttta ctttcaacgt ttcgccaatt tttaccgtat gtgtttgatt
    19921 attaaaagtt gctttttcac caaagttatt aatcaaacgt tgaatttctg cttttcgacg
    19981 atcataatca acacggatat ttttgattgg gatttttgtg cccaaagctt cccaaatcat
    20041 aaattgtgcc gcaatgtatt gttcatcgga tttgtcttta tttgtgacat atcctaaata
    20101 agagatcaag cttgtttttt tccgtacatt tgaatttttc aaatagtttt tcaaagcagt
    20161 agctgtataa acttgacctt ctgcggctga gacaccaggc tcaatacaaa acgccacttg
    20221 tccattaatc tcaaaacgaa ccatataatt taaagtgaag tttagattgt tcggactaga
    20281 agtaacacca atattttcgc cccaatcaat tttccgacca cctaaaacag ctgcattggt
    20341 tgcacggcga acacgaaccg aacgattggt tggtactgtt actaacagtg taggtgtacc
    20401 tgtttttgga atgttctgct cttttttctt ttgattttca atactagccg tagattcttt
    20461 gtttggtttc gttggttgat tttgactagc tgcggttgaa gagattgttt tttcaacgtt
    20521 ttcctcattt tttccttctt tgtcagcagt agccgctggt tgttccactg tttcttctat
    20581 tcgtttcttt tcttccgctt gtttctcaga agactctggc tgcacagaag gcgttgcttc
    20641 ggcagctttt tctccttcta gagtggacgt ttctgaagca ggggttgtaa ttgaggcagt
    20701 ttcttcaact ggttgtgaag taccaggact attccctggg tgttgtgttt ccccaatcgt
    20761 ctgatcgaga gtcgttttgg aattgaccgt tggcgcttct gtcacagggg cctgttcaac
    20821 agtggcttca ccagctggga ccccggcttc tgctggttgg acttctgtca cttcagaagc
    20881 gacttgggca atggcttcca tcgtaggttg tatagccgtc gtttcttctg catgtgcttg
    20941 agcagttccc accaagaaac agaattgaga aaaagccacc aatgcgagtg ctttcaacca
    21001 attcattttc gtttttgcat acacaagaat tcctcctttt cctgagaatt ttatataata
    21061 aaacaaaacg ccgttaccaa aagtgataac gacgtgaact gtctcttcta tttttatctg
    21121 gcgctcttat ctgaacacat ttcatcaatg attttaccct cccactataa ttacagaaca
    21181 aaaaaagaac ctgcttcgtg gaaatcaact gtccatttcc caattggaat accagctttt
    21241 ttggcaactt caccatcacc aaatctcatg ccataccaat tactaccttc atccttaatt
    21301 tctttccaac catatgacat agcttcatct aatgaatcaa aaatcatgac actattgcca
    21361 attgttacag gatctggctt aagatcttct ggaagagaaa tatcaatcat accataatga
    21421 tccgattcct cagtattatt gtcattacta gctactggtt tggttgttct actggcttat
    21481 tttgatacaa caagtactct tattcttctt ttacttctcc gttttcaatt tcccattcaa
    21541 accactccgc aaagtcttgc ttaaaaccat ctactcgata cctattttgt aaataggcat
    21601 cttctgacgt cgtaggatct aaataggtag gtttattctc tggacgagca tagacaccta
    21661 cccatgtttc gtaatctcct tcaaaataac tatctaccaa atagcctttt tttctaagaa
    21721 tctcccgaac ttgattttta attattgata cttgttcaaa accatcactc attctatcac
    21781 ctcaaataac tctagattct cataaatatt cccgacaatt tcaatcctat caatgatctc
    21841 taacaaatcg ctacttatgc ttccccattc gtataagaat ttcccttccg caaaataaat
    21901 tttccataaa cttcttcatg atcgtcccaa ccaatatcac tctcgaaaaa ttccacgttg
    21961 ttcttatctt ttagctcagt aaatgccata agttcaacat gttttccaac ctcgaaacaa
    22021 tcacaccacc catcaaccgt tacacgtgtg ccttcaatac ctaagaaact ttgtcctcgt
    22081 gtattaaaca ctaaaacttt ccgcattttt tgctcattaa ttaatcacgc gcgaaatcta
    22141 ggaatcacaa cttattcgct tcttctttat aaatcgccga ttgtaaaatt aagctagaca
    22201 actaaaaaaa gtcatttgtg ctacactcaa aatagttccg acgaaagaat tataaggagt
    22261 gagtacaaat gacctataac catcttacac caactgaact cgtaatgata gaagcttatt
    22321 tcaatcaatc acaacctgtt tcaaaagtag ccaatctgtt acaacgttct cgacaaacta
    22381 tttataaggt ctatcgtttt cttaaatctg gtggaagcgc aatgagctac tataaacaat
    22441 ataagaaaaa caaacgtaac tgcggaagac atccgattgt tttacctgat gagcaacagg
    22501 aatacattca aaaaaaggtt gttcaagggt gggctcctga tgtccttatt ggtcgagcag
    22561 agtttcctat tgcttgttcg gtacgcacac tgtatcgaat gtttaaagat aaaacctttg
    22621 acactcacga tttaccaatg aagggaaaaa gaaaaccaaa cggtcataaa gaaaaaagag
    22681 gaaaacaagg gtttcgccgg tcaatccgag acagaaagac agactatacc cagtttgatc
    22741 atgaatttgg tcacctggaa ggtgacacta tcgtgggtgg aaaacataaa agcgcggtca
    22801 tcacattagt tgaacggcta tctaaagtca tcattacctt gaaacccgaa ggcagacagg
    22861 ctaaagacat tgaaaatagg ctgaatcaat ggtttcgttg tgtcccaaaa aacttattta
    22921 aatccatcac atttgattgt ggaaaagaat tctcaaattg gaaaaagatt agtaatacca
    22981 acgatatttc aatttatttt gcggatccag gaacaccttc tcaacgagga ttgaatgaac
    23041 attcaaatgg cttattacgt aaagatggtt tgtataaagc tatggatttt aatggtgtat
    23101 ctgaaacctt tatccaatcg gtggcatcga aacgaaatca tattcctaga aaatcattac
    23161 actatcggac accaatggaa gtatttttga gttacgtcac aaaagatgat ttgtctaact
    23221 taatttgaca aacgaaactt aataaaaaag gaagaatatt catgtaaacg attttattgt
    23281 ttttttctct tttatacaaa aagggtcata atttcaagta agcggatgaa ttcatgagga
    23341 ttttttgcat actgtctcta ctatacttag gtcactaaga aaaaggagtg atttattatg
    23401 aaaaagaaca tgattgggac attattactt tcaacacttc ttctaggcgg cttggcaaca
    23461 ccagcttttg ctgagggtca agcgacttca aaaggagata ttacctttac tgaaccaaca
    23521 aataccgtgg agcctcttaa ccctactgat ccttcaaaac cagtagagcc agcagatccg
    23581 gaaaatccag caactggcca gacaggtcct ttaacactgg atgtagttcc cgaattgcca
    23641 tttggtacac acgagattga aagcggaaca aaaacgtatc aagtagacgc ttcaaaaaac
    23701 gatacacctt atctccaagt atcagatcgt cgaggtgtag gtgcagatgg ccaagcacaa
    23761 gggtggaacg tgacagtttc ggtttcagat tttgtaaacg gttcacaagt tttgcaaggc
    23821 gctgaattag attttggaac atccactgtt aaaagtacgt cagataacga atccactgga
    23881 ccaactagtc aaactgtaac tgggttgagt aaagcaagtg cagcgacacc aatatttaca
    23941 gcagcaaaag atcaaggtct gggaacatgg ctttctgttt atgatccagc aaatatcaca
    24001 ctgaaagtgc cgaaagcagc agctggaaca ttcacggctg atttgacttg gaacctcgtg
    24061 gctggcccag tagcatagag tatgaaataa attcaacaaa ctgcggtgaa aatactatag
    24121 gtgaaggatt ttttaccgca gttgttgttg aaaggatgtg gtggacaatg aatcaaagta
    24181 aacgactgtt tgtttcgttt ttttctattt taagtctatt tttcattttt ccgtcaatca
    24241 gccaagcaaa ggattcagca ggagactttg gaatcaaacc ggtatttcca gagaaccaaa
    24301 tagataaagc aatcggttat tttgatttat tagtggcacc ggaacaaaat cagacactag
    24361 aagtaattat cagcaattct tcagatgaag aacgaacatt tgaggtctct gtcaatccgg
    24421 cggtaacgag tgatggtgga acgatcgatt atagtcaaaa aaacccaacg ttagatgaga
    24481 cattgccgtt tgatgtccgt gacgttttgc tcattgcgaa aaaggaaatc aatgtatcag
    24541 cacatgcaga aacaactgta cctatagaag tgaaaatccc agcaaaaagc tttaagggac
    24601 gtgtattagc gggtattcat gtttcaccta aagaagaagc tgaaacagaa aatgctacag
    24661 aaggagcaca aatcaaaaat cgtattgctt ataatctcgc agtcgtttta caagaaagtc
    24721 aagagacgat agaaccggat ctgaaacttt tatcaggaga tttggacgag gtcaacgcaa
    24781 agccaactgt tcagcttcgt ttccaaaatc ctcagcctag gattatcagc aatttgatat
    24841 ttacgagcaa gatcttttat gaaaatcagc tttatattga aaatacgtct aatgcatttt
    24901 tagttgcacc aaattcgaat tttcacttga atcttgattt agctggtgat aaagcaaaag
    24961 ccggcgatta ccgcgcagaa atcatagcaa aatcaggcga cagtaatgaa tggcgattta
    25021 cacaaaactt tacgatcaaa aaagaaaaag cacaaaaagt aaatgaaaat tccgtttttg
    25081 cagtacaaga acaatctttc ccatggcttt atgcaggatt ggttgctgca gttctagtgt
    25141 tatggttgat tttgctgttt ttgtgggtcc acaagaaaaa gaaaaaagga gaaacaacat
    25201 gatgaggtga tttcgttgaa aacatcgatc ttattgggag ggattattgc aagtttgctg
    25261 ttcttcagct ggcaagagcc agtccaagca gataccaaaa gttcaggtac gattacattt
    25321 tatgatctaa gtccaattcc gcatccaagt ggttcagtca atggcgcatc aaaacagcag
    25381 acacagctac cgatttataa agaatctaat caacatggcg tattcccgga gacgaatgaa
    25441 caaaagatcc gttttcttta tttgatcatt ggtatagaat tatggcttct tgcgtggttg
    25501 ttgttatgga gaagaaagga aaaggaggat gaacatgttg ctacaaaata aattttcatt
    25561 ttttcgctcc ttctttttta cttcgctttt tgttttaagt atatttatct tttcaccttc
    25621 ggcagaagct gttggagcaa cgcctcctgc catttcttat gatccataca cgtatgaaaa
    25681 tatcgtacct ccaaatggtg gacgtagtgc agtcaatggc gggaagcttg tccaactaac
    25741 tggggctaac caattgccta aattgactgc tacttatttg aacgatggtt caaatgatcc
    25801 ttggaaaggt ggcacggtcc gttttcattg ggaaagccat gtgaaactaa caaatgtact
    25861 tgatttggtg aatatcactg cacaaggatt tgcatttgtg gcgatcatca atttgccgga
    25921 acatgtatca agcagtgatg tattaaaagc aattgactgg aacaacgcct acttgtatat
    25981 tgccggtgcc cccatcaagg ttactagcgg aagtttatta tcagcaggaa gccatacctt
    26041 gcgttttggg atggggtcgt gggatacaaa tactctattg acagatatta ttgctattat
    26101 tacgcgaggc gaattcaacg tggacgatat cccgcttcaa tttgatgtta cagctaatgt
    26161 cgcgaagatg acagaaaacg gagataaata tgatacgagt ccaaacaaaa ttttgaccaa
    26221 gggtaaatta caaccttcta tgtctaaaaa ggccgttttt tctgttgatt tttatgattc
    26281 gaataatatt atccaagatc gtatgttcaa ttcgatttta gctccagctg caggtagatt
    26341 ttatcctaaa ttaactggcg gaaacttgaa tccaggagca tatgcatttt ctgcaagtgc
    26401 agagataaat acgtggaata gttacatctc accaagtgat tcaacaggaa aatataatac
    26461 atttgctgat accacagaat acgtggatgg aaataaccag aaccttccag gctctaccaa
    26521 tagtcggaca ttgacgatgg ggctgactga aggacgtttt gatcagttgc catttagtcg
    26581 ctttgatcgc gtagtcaact attttacagg aaaaaacgtt acacaaggag caatactgac
    26641 tcatactcca gcaagcacaa gtggattgaa tcagacagcc aatatcgtgt acagtgggaa
    26701 agatgcgcaa ggcggattgc tttctcctgt tgctttaaag gtattggagc agtacaagct
    26761 agacggaacg gttttaccaa caaacttaac agccgatagt cagtggggaa agctcctttc
    26821 ttctgcatcg taccaagtca cggccaattg gtcagcaact gaactaaaaa agggttcgat
    26881 ccattacagg ctttattcga aaggaaccca aaaattagcg acaggatgga cagatcaact
    26941 tttccaaacg atccaatcaa ataacggggg tgtaaataca gctacttcta cactacctgc
    27001 attggcttca gggcaatatt attttgatta tcgcttggtg gacgatgtac ttactaacat
    27061 gtatccgtct tttgcgtaca aatggcagtc tgaacaagct ggaatcacca ctcttcctca
    27121 aataacagtg gctaattttc catctgtgag tgcagatagt cagctaaaaa accttagcag
    27181 acaacctgag acaggagatc ccctgtctgc tctatcagga gatacgattc aagaaaatgt
    27241 gacattcacg ttaacaaaat ctggggatgc gataaccgac aaaaaaatca cgatctcact
    27301 tcctgaaaat actacttata tcaatggttc cttaaaatta aatggtacaa ctatcgcaga
    27361 taccggcatt caacaaggag tttctgtccc agcagatcta ttgagcaaaa taggagatac
    27421 cattcatctt acttacaact atcaattaaa tactgtagat acttctttac aatccgtcag
    27481 catactgaca aaggcagctg tactttccag caatattaca ttagcagatg gtgctaagct
    27541 tcctaaccca gttgtccaaa catctgctaa aacaattctt gtccccaaac aagaattaac
    27601 attagttaat gtaccagatg attttacatt tggtaacgat ctccccaaac cgttgaagac
    27661 aagttattat gaatcaaaag gcgatttttc ttttgatgtg cgggatactc ggcttccaag
    27721 cacgtctcct tggcaactga caggtacatt gacttcttta tttaagaatg atcaaggtca
    27781 agaattaagc ggaacaaaat tatacttcaa tcattctgga tcaaagcaat tgattcagca
    27841 aggtcaaaac acgttgattt atgagagtga tggaacagct aaaggagagg ttctcgttga
    27901 ctttcccgat accgatggtt tactcttaga ggtaaatagc agtacgaatg ctcaacctgg
    27961 cgcaacgtat caaggcatgg tcacatggga actgacagct gggccaacca gctaagtgat
    28021 tagaatttgt atggatctgt gataactgtc acagatcctc tttttcaagg atgagaagga
    28081 aacttctgct gttaaaatca gaaatgcttg aaggagtgga cttggaactt ttcagtttgc
    28141 ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact tttattagag gaaaaaggag
    28201 aggtttccgt taataacgta cttacccgct tatcagttga tcgggctacc ttgaaagaag
    28261 atctgtttta tttacaagct aacttggaaa atatcaaaca attggaaatt gttatccaag
    28321 aagggaatat tttattaatc agacatggaa atatagcagc agtcgatgtt tattatcatt
    28381 gttacttgcg ctattctaca aaatatcaga tacttcttta tctgttggaa aatggctcat
    28441 ttgatcggca gaaacttgtc atggaactta atatcagttc ggctactttg tctcgcagag
    28501 tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa aataaaaagc gggcggctga
    28561 ccggtccaga aagtcaaatt agttattttt atttccaatt attttggttt ggacgtccct
    28621 atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa cactttttta gatctgctgc
    28681 aaaaagagtt tagttttgct attacagaag aaggacgatt gaaatttgaa atatggcttt
    28741 ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa accagattat agcaatcttc
    28801 ctgttggatt gtttcaaacg acacaatttt tattggacct ccatacattt ttgacccgtt
    28861 acttctttca atatgccttt ccttggaatc aaaatgaaac gttggttttt tatcttttca
    28921 tgatcagtaa ctttacatta gatacacata actcgcatgt ccaggagttt ttgataagcg
    28981 aaaaatacaa agacgacgta gtagctcaat tgaatcgaac gtttaaacaa tcgatagaag
    29041 aatatttctc tttttatgtt tttccagaag agatttcgca aaagattgcg ttgacagtga
    29101 tgcaaaatca cgctcatttt gcctatttta gaggatggat gaatattttt ggccaagaag
    29161 gattaaaaca gcggttaact gaagtagaag ataaacaatt cctggtttta tgtgaaagat
    29221 tggtcagaca aacatttgct attgttgatt ttactgaagt agacgaggaa aatgcccgct
    29281 ttgaattatt tgctcgctat gcatctattt tacggacgct gatcaagcag ttagaattac
    29341 gtctgcaaat tgcttatgat cttccttatg aaaggctaat ggcggatatc gtgaaagaaa
    29401 tcatccagga acaattgctg aatacgatca aatatgattt agtcgaatac gagaaagaaa
    29461 agcagtatga tatcattttg actagccaat tgaaagagta cccgtcacag aagtccgcta
    29521 aagtttttgt attcactagc aacgaatata attatgattt cccctatttg aaccaatttt
    29581 taaaagaatg ttatttagaa aaattgaatt taaggatgaa taaaacatga aaatcgtgat
    29641 cattggtgct tcccatgaag gcatcacagc tgccctaaat ctaagaaaac ttcaaccgaa
    29701 agcagaagtt ttactgatcg atgaagatca taaagatgga ctaggctatg tttcaaatgg
    29761 aatcaacctt tatttaaaag gaaagatacg tagtttagct gaagttgccc ataacatgcg
    29821 gactcttcaa gaaagtgggg caaaactgat caccgaatgg agagtaactg aacttgatcc
    29881 agacaaacat cagctcatcc tcgcatccaa agaaggaaaa aacgagacca taacctatga
    29941 taaattaatc gtagcgacag gttcctctcc tgtcaccttg tataaacaaa ttgaagctga
    30001 aaatgtctac acctataaaa atctagtgca atctaagcaa gtacttgccg ctttaaaaga
    30061 agcaaaagaa gtagtgatct tcggtgctgg ttacattgga ttggaattag cagatgcttt
    30121 gagaaataaa ggatacatta ttcatctagt tgattatatg ccgaatgttt tatcaagata
    30181 tttcgataag gatatgatca acagttttca aaatcagctg caaactaagc aaataaattt
    30241 ttatccaaat gaattcttga tcgattggaa aaaatcagaa gagaaagtag tatccgtaca
    30301 acttctgtct caaacaataa aggcagatat ggtcattttt tctgcccaaa ctcgtcctaa
    30361 tacgacattg ttaaaagaaa aagtggcttt gtatgaggat gagactgtga tagtcaatga
    30421 atatctgcag acaagtgatc cggacatcta tgcgattgga gatatcgtcc cagtcagttt
    30481 tgacaaaaat aaacggcatc tctttttacc tttagttact cgagctgtac atatggcaag
    30541 agcagtagcg ctaactttga gtggtcagcc aaccgcttac gacctcagac aaaagataac
    30601 agcagcagtc attatagact actttttagg aactgtaggg ctgacagaag atgaagcgcc
    30661 attcttggaa caaaataccg gttcgtgttc aggagaattt gatctatttc cacaatacga
    30721 cgaagaaaac aaaacagtaa atgcaaagct gatctaccat ccggatacat tagaaataat
    30781 tggcggacag ctgataagcc aagaattctt attatcagat ttgaacctgc ttgcggatat
    30841 cgtgaaacac aaaactacga ttccacagct agcagttgag gattttggat ttctgtctga
    30901 atacacctct cgttttcact acctcaatga gttagctttt aaagtactag ctaagagagc
    30961 aaatagcaat agagtagata aaagactgtg agaaatatca cggtctttta tt
//
LOCUS       DAZKOB010000020        30380 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000020 DAZKOB010000000
VERSION     DAZKOB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.020"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(372..1682)
                     /locus_tag="ACG8M6_000964"
     CDS             complement(372..1682)
                     /locus_tag="ACG8M6_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0456651.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1694..3052)
                     /locus_tag="ACG8M6_000965"
     CDS             complement(1694..3052)
                     /locus_tag="ACG8M6_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0456652.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3254..4822)
                     /locus_tag="ACG8M6_000966"
     CDS             complement(3254..4822)
                     /locus_tag="ACG8M6_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0456653.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4826..5236)
                     /locus_tag="ACG8M6_000967"
     CDS             complement(4826..5236)
                     /locus_tag="ACG8M6_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0456654.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5233..6156)
                     /locus_tag="ACG8M6_000968"
     CDS             complement(5233..6156)
                     /locus_tag="ACG8M6_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0456655.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6153..7031)
                     /locus_tag="ACG8M6_000969"
     CDS             complement(6153..7031)
                     /locus_tag="ACG8M6_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0456656.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7018..8355)
                     /locus_tag="ACG8M6_000970"
     CDS             complement(7018..8355)
                     /locus_tag="ACG8M6_000970"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0456657.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8358..9461)
                     /locus_tag="ACG8M6_000971"
     CDS             complement(8358..9461)
                     /locus_tag="ACG8M6_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0456658.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9535..11043)
                     /locus_tag="ACG8M6_000972"
     CDS             complement(9535..11043)
                     /locus_tag="ACG8M6_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0456659.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11009..11701)
                     /locus_tag="ACG8M6_000973"
     CDS             complement(11009..11701)
                     /locus_tag="ACG8M6_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0456660.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11723..12697)
                     /locus_tag="ACG8M6_000974"
     CDS             complement(11723..12697)
                     /locus_tag="ACG8M6_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456661.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12997..13869
                     /locus_tag="ACG8M6_000975"
     CDS             12997..13869
                     /locus_tag="ACG8M6_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456662.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14327..16525)
                     /locus_tag="ACG8M6_000976"
     CDS             complement(14327..16525)
                     /locus_tag="ACG8M6_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0456663.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16757..17749
                     /locus_tag="ACG8M6_000977"
     CDS             16757..17749
                     /locus_tag="ACG8M6_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0456664.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17809..19230
                     /locus_tag="ACG8M6_000978"
     CDS             17809..19230
                     /locus_tag="ACG8M6_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0456665.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19223..20722
                     /locus_tag="ACG8M6_000979"
     CDS             19223..20722
                     /locus_tag="ACG8M6_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0456666.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20761..22155
                     /gene="uxaC"
                     /locus_tag="ACG8M6_000980"
     CDS             20761..22155
                     /gene="uxaC"
                     /locus_tag="ACG8M6_000980"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0456667.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22314..22997
                     /locus_tag="ACG8M6_000981"
     CDS             22314..22997
                     /locus_tag="ACG8M6_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0456668.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22999..23835
                     /locus_tag="ACG8M6_000982"
     CDS             22999..23835
                     /locus_tag="ACG8M6_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0456669.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23846..25600
                     /locus_tag="ACG8M6_000983"
     CDS             23846..25600
                     /locus_tag="ACG8M6_000983"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456670.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25876..26217)
                     /locus_tag="ACG8M6_000984"
     CDS             complement(25876..26217)
                     /locus_tag="ACG8M6_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0456671.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26285..28456)
                     /locus_tag="ACG8M6_000985"
     CDS             complement(26285..28456)
                     /locus_tag="ACG8M6_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0456672.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28667..29524
                     /locus_tag="ACG8M6_000986"
     CDS             28667..29524
                     /locus_tag="ACG8M6_000986"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0456673.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29595..>30380)
                     /locus_tag="ACG8M6_000987"
     CDS             complement(29595..>30380)
                     /locus_tag="ACG8M6_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0456674.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9079 a         6258 c         5380 g         9663 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt ttaacgactt agttatctta tcacgtctaa
      241 aaacatgtgt caactctttt ttggaatttt cataaaatgt ttcaatgaag cgcgacaacg
      301 ttgtttaata tatcattaaa aaaaataaag gtcaagctaa aatattattt aagcttgacc
      361 ttattataat ttcaacgatt gtcaaaagta ttgtttgcag ctgtgttgcc agtttctata
      421 gataaatcac agcttttgaa acataagttt tgtactgctt cgattcgccc gaattctttt
      481 gcagttattt ggcaatgaga aaagcagaca tcttctatag gcttttcagg aaatgctact
      541 aaatcaaaag ctcgggaagg aagtgagtaa cctggagaaa ggcatgcctg tacgtttttg
      601 atgtgtatgt ctttgacttt cgtcatttgc tttgattcag gtatttcttc agccactgct
      661 tgccaacttt ctggcatttg agcaaggtca ttgctatttt ttctattgta ttcattatgc
      721 cagtccatta tccaagaaaa aggaaattgt acatcaatca tattcagatt ttctaccctg
      781 atattttcga tcactccccc tctttctttt gaagatttca ttcgaatacc acagtctgac
      841 tgaaaaaaat cgatatcgtg aatatatacg tcactaactc cagcactaac ttcgctacca
      901 atagttacac cgtatcctga acggatttta catctagcaa cctcgattct tgatgatttt
      961 cgattgactc ttgcaccatt gccatctctg ccagacttaa tagcaataca atcatcacca
     1021 caggccaaat cacattcata gacacgcaca tttgttgaag aatcaatatc aatcccatct
     1081 gtacttggtc catcattgtt gcggatgatt actttttcta ctaatacatc gttagaataa
     1141 gtgatttgca agttccaaaa acctgatttc tgaaaaatca aatcacgaac aacaacatgt
     1201 tctgcatgat acagtaaaca agcgcgtgga cgtttaatag cataatccgc aatccaacga
     1261 agtccctttt gatcgtattc agctcttgtc ccgctttttt gatcttttcc ccaatacagc
     1321 tcccaccaat ggtctccttg accgtcgatt attccttttc cttctataag aatatctttc
     1381 gccgagagaa cattcaaaat cgctgctggc catttcattt ctacgccggc tactcgtgta
     1441 tcgatttctg gaaatgcttc gatatctgtt ctccccttga ggactgcacc ttcttcaaag
     1501 ataagatgac tcttatcttt taaaaaaagg gctccgacta aataaatgcc ttttggaata
     1561 aatacaggta agtgttcttt cgcaccttga tcgattgctt tttgcagtat tattgtatca
     1621 tcttgttgag tgtcgtttgt tttaaagttc aaagcattga taatcccttt ttttgtccgc
     1681 aatacgtttc tccctatctt tcattgattt ttttcaattc ttcaccaata aatacagttt
     1741 ttgaacgatt cagtgggtaa cctactagca aaatgattgc ggatacaaat aagcagaatg
     1801 ctggtaacaa gtttgccatc gtataaatcc tttcttgtac taaaactgat tggatggctc
     1861 cacctgttgt agacgattgg taaccaatga gtgccagcat aaatccgcct accgctcctg
     1921 cacatgcttg tcctactttt ctcgcaaaag aatttactcc atatactgtt ccatcttctc
     1981 ttaagcctgt cttatattga tgataatcaa tcacgtcagt aatgaatgcc cagaccatca
     2041 aattgaataa accagatcct aaagtagcga taaataaaag aatcagatat acccatgcat
     2101 ttgttatatg aaggaaatac atcagcaagt acatacatga agaagcaagt aacgcaaata
     2161 cgcttgcttc ctttttacca aataattttg tcagctttgt agcaaatggt gctaaaacta
     2221 ctactgttcc gtaagtaaat aaaagagcaa cagacattgc ttgtttatta tggaagtaat
     2281 cattgaataa ataagtcata ttcgtcccag ctaaaatctg attgatcaca atgaaaatgt
     2341 caacaatgac taaaataatc agtgctcgat tagctaacaa accttttacc aactgagaag
     2401 cagatacttt ttcgtttttg acaactctta ctctttcagt agtcaattta caagttaaca
     2461 tatatgcgac aaatgctatt gcagcacatc cgattgaaat ccaaaagaaa tgattccctg
     2521 aaacgacttg ttgtccactc ttgttatggg tatacattaa aatcggaatg aagaatcctg
     2581 tagttgatcc gccaatcgca gaaccaatgc ttcggaacgt ggacaacgag gtgcggtcat
     2641 taggatttga gctaattgct gctgccattg ctccatacgg gatattgatt gttgagagaa
     2701 aaatcccata aacaatgtat gaaacaaaaa tataagcaat cttcaccgtc attggaagat
     2761 gttggacgaa tggcaaaaac aaaatgacag tcactaagca aaatggatac ttcatttttc
     2821 tgatccaagg aataaatcgg ccagtttctg ttaactctga gacatcacat aatcttccaa
     2881 ccatcacatc agcaaacgca tccaaaattc tcgaaatcaa gaataacgta cctacgattg
     2941 cccctgaaac gccaagaaca ttcgtataat aaatcatcaa gaaactattg actaaaccta
     3001 aaataaaaca gttacctaga tcaccgaaca tgtagcctat cttatcggaa atcccaaatt
     3061 cctttttttc ttgtttttat attcacaatg ttggcttttt attgcattat cttgacagat
     3121 aaaaaacaaa aaataatcta aaaacattga tgcataagat tgataacttt ttttacataa
     3181 caatatatac aaacaaaaaa attcactaac tagtgaaaat aagttagtga acctcattta
     3241 ttaaagaatt aaattacaga tgatcttttc catcatactt gaaaaactca aaatcagcat
     3301 aacttcctct ttttttattt agatcatgca ctgctatccc gataaaattt ccagtaaacc
     3361 ctcctgagag tatacacatg tttttttgcg gacctacttt cttccattct tcagttgata
     3421 cgtctttcaa atgaaactgt gctgtctctt tctttacagt gactttcaaa aaagtcttct
     3481 tttctgtgag tggaatcgtc caatcttcta tttgtgctac tccgttttct gattctatca
     3541 tacgtaggca ttttcccttg tcctcattcc aagtaacata acaataaata taattggatt
     3601 catttaagta cagtaccagt ccagccatct gattaaaatt attaggcatg tattcaaatg
     3661 ctgtctccgc atcaaaagta aaatccgttt gccgaatagc taacaaatga tgttcgaagg
     3721 tagattgaag agattcaccg gaaatcagac gtaaaaaacc tggtctacat tttgtatcac
     3781 accaatcagc atttggcaaa atgcgtctgg cattccagtt cttctttagc tctccttcaa
     3841 atgtatccat aaactgatga tctttttctt gttgtacctc ttcatttgtc tcaatcatta
     3901 tttcttccat cggagttgtc tggctatctt taagtcttaa ccaatcgtcg gacgtccaat
     3961 acacttcttg aatagcggtt tctcttccca aaatagccgc accgtttaat ggtctggtac
     4021 aaagatatgt catgtaccat cttcctgacg gggtttgtac taagctccca tgtcctgtac
     4081 attgcaatgt agaagcaggt ttgtcactag ctgtcagcat aggaaaacca ggatcaagct
     4141 cgtaaggacc agtaatattc tttgctcggc aaactgtaac tgaatgtcct cttcctgtac
     4201 cgccttctgc cgtcatcaaa taataatacc cgttacgaaa atacaaatgt ggtgcctccg
     4261 ttttcgctaa ttctgtacct gcaaatattt tatagatggg accatcaagc ttcttcttct
     4321 ctgcatcata ttcttgtaat acgataccgc tggatttatt cggtgtgtcc agacgataat
     4381 cccatagttc attgacaagc catttttttc ccgtttcaag atcatggaac aaagaaggat
     4441 caaagccgtt actatttaaa taaattggtt cggaccaagg acctgtgatc tcagatgcag
     4501 tgatcaaata attgtggaca tctttaaacg gacggcctgc acttttgaca tctgtgtaaa
     4561 caagataaaa caatccatca gcataactga tctgtggcgc ccatatactt ccatttgtag
     4621 gatttccttg caaagatact tgagtggtca aaatatccgt tgtatgtgac cagttgacta
     4681 aatttttcga ctcatatact cggattcctg gaagccattc aaaagaggag acaataatgt
     4741 aataccgctc tttcactctt aatatattag gatctggatt gaatcctctt aaaataggat
     4801 ttttcacttt gatagtctgc atgattcagg ctccttttct cattcttact cgatataaac
     4861 tgaatgcccc agctgcgaaa taaaaaaatg caaaaggaaa cgcaataaac tgagtgccaa
     4921 tcaacaatat gaatcctgtt ataaaataca aaccagtggc agttgttaag tattttcctt
     4981 tagcagtcaa aaacaatgct actgctaaaa aagcaagtga aataaacaac acaacggcaa
     5041 acagaccagt catgtcctgt aataatccaa atgtattttc taaagacaaa gttgaatgat
     5101 ctacagcaag cgactgataa ctttttttaa actctgacgc agacatatta cttaaagtaa
     5161 ctgctaaccc gccaaagaaa atcaaagata gactgccacc gaccattcct agcaatagtt
     5221 cccattttct tttcatttct gaaccccctg cttttctaaa tgtatccatt caggtctttt
     5281 gatcgatgtt tcataattgt tattacgttc agagaatttg acagtctgtt catttttctt
     5341 gtcatcccaa ttatcccaac cagttgggtg aacatgccca cctatcttac acgcttcaaa
     5401 taagacattt gcatacggac gccacggtct tcctaaatag acgtttctag tatcaggttc
     5461 tgcagtcaag gtacagcttt caaaacgata ccctgttttt tgatttttcg gcgtagatgc
     5521 tgctgtgatg tatccaggat ttttactggc taagctatgg atatgacact gatgaaaaac
     5581 ggcatctgcc ccaccaaata tgaaatccac cgttccttca atccagcaat attcataggt
     5641 ctgacgacaa tactcaggat tggcagaagc cggtgtacaa aaaggagtac catctttttg
     5701 caaatcaggc aacggacccg tgcataaggt atcctgataa ccagatagtc gacagttttt
     5761 taaagatacg cgatctcctg aattgaataa agcaacagcc tgtcctacct tttctcctgg
     5821 acctgctgta ttttcgatgt gaagattttc taatacgaca ttcgatcctt ctaagaaaaa
     5881 agtagcactt cggaaagtcc ctaactggct accgtcaaga tactcctgtt ttgcaaaacg
     5941 attaccggag ataactactg ttcctgctcc cacaagttta aaattggata atctgctttc
     6001 tatatattca tcatatcttc cttcttttac tatcaagacc tttggatggc tatccggcag
     6061 gcctgccaaa taatcgattc cttcttggat agtttcaaaa tccgtcgggg tatctatagc
     6121 attctttcca attatcagtt tgtctttcat cctcataaat gctcccttat gaattgtttc
     6181 caaatatttc tcatttcttg tgtttccata tgaccgcctg ttaaagaaaa agaagaaaag
     6241 ttttctggac tacccattga ttgatacttt ttgtccagcc cttttctcag cttcagtacg
     6301 ccttcgtcca agcacatcgt atcgtttttt ccaactaagc taagacgagg gcgcggagcg
     6361 attttttctt ggattgctaa cgttgtataa gctgtcaata gatttggcac atagtaataa
     6421 aacccatgat gatctaggcg gcgatgtagc aaaagtattt cgatgtccac ctgtgccgct
     6481 agatcaatac aaactttgac acgagaatcc aatgctgtca cccaccagct taataagcct
     6541 cccatagaaa gaccaatcgt tgcgagccgt tgagtatctg tgtcttccct tgtctccaaa
     6601 taatcgatca agctgatcac atcataaata cgcattcccc ataacgtttt tcctgttaat
     6661 aaaaattgtt tgaacaactc gctttcagaa atacctccgc gttcttcaaa tccccaagca
     6721 tcaatcgacc aaacagcata tcccattcct gtcagtactt ctgcaaaaga tggagaagct
     6781 aagtaaggag caccatggat caattcactt ttcccgactg aaaaatctcc gccgtgagaa
     6841 tgcaggtaaa ggaccggcgg aaacggtcct tctttttctg gataagcata gagggctggt
     6901 actgtttcca attcatttaa gtacaattga tagtattcaa gcataaaacc gttttgtttt
     6961 tctcggtata atcgttttcc actaggggta tgtctgctag atggagcacc taaaaaatca
     7021 ttcaactgca taagcaaagt ttttacgaac aaccagttca tccatatttt ctgattgatt
     7081 tgattggaat ttctcaatac tgatttcttt agtattttct aatagaaatg ctggtccctc
     7141 gtgattttca atcgacatct ggttaaagga aaccttaccc gcaaagccaa tataaaatcc
     7201 ttgtctgctc atttcctcta ttcctgtcat catagcagga acaccgggaa ctgcatcttg
     7261 ggccatcgag acatcgatat tgtcaaatgt gatttcttgt atgaattgtt ccgctaatcc
     7321 ataaataaaa cctgcagaag catggacatt ccttgccgtg atattcgaaa aatggatcct
     7381 tctgaatgct ggtgtccgtt catctatcgg ataagccttt ttttcccata catacggttc
     7441 tttccctcgc ggaccacaga aataataaag attcagtata aatggacaca tcacattatc
     7501 catgactata ttggatactc gaatatcttc aacgatcccg cctcgacctc gtctagattt
     7561 caatcgtatt cctcgatcag tttcttggaa gatacaattt gaaatggtaa tattacgaat
     7621 actaccactc atttcacttc ctaatacgac accgccatgt ccatggacca tcgtgcaatt
     7681 tgtgatcgtg atattttcgc aagcgatcct ttcgtacgta tcttcagtcc ctgccttgat
     7741 tgcaatacag tcatctccca cgtcaatatg gcaattacta atacgcacat ttttgcatga
     7801 ttctggatca attccgtctg tatttggtga atccgctggg ttcaagatcg tcaagttatc
     7861 aaacgttgca ttactgcaaa gaatcgggtt gatcgtccaa cttggtgact ggatcaattt
     7921 aatatctttc acagtaattc gatgacagtt atggaaactc atcaatttag gacgaggata
     7981 agcgagatta tctggttcat ttctaaaagt atgccaccat ttttttccat ttccatctaa
     8041 tgtaccgaaa ccagtgacag agatgttctc tacattttga gcatagatac aggaagcata
     8101 aacttttcga tggactcctt cccaacgtga atgaacaaca ggataatctt ttggatcgtc
     8161 agagaatttc agaaccgctc ctgcgcttaa atgaagttcg acattgcttt ttaaaaataa
     8221 agctcctgtt agaaattcac ctgctggaac aactacggtc ccgcctccgt cacttgctgc
     8281 agcatcaatg gcttgttgta ttgcttcagt attcaattca tcaattgatg cgccaaatgt
     8341 caaaatatca tacatattta taatgcctcc acttcaatca atgcttgcaa gaatgcacca
     8401 acgccttttt gatcattaca aataattggt tcactgatgt agtaagcata tgaaccatct
     8461 cgctgatctg ctccgcctaa ccctgctact tgacagtttt tattcaagtt cagccagcct
     8521 tcttttgttt ccagaacaaa ttcatcaagt aatccttgat gtgatttctt cacttgtgga
     8581 agccattctt ctttgttcag cagtcccaaa cgtattcctt tagccattgc gtaaacaaac
     8641 atactactgc cggaagcttc cagataattt cctttccgtt ttgtttctgt tgttacttga
     8701 taccatgtcc cgctgttttc ttcttgatat ggaagtaatg cagccagtgt cttttctaga
     8761 atttctttca gcaaagaaca atctgtttct tcatgaagta attcataggt atctgctgca
     8821 gccatcaaat accagccaat cgatcggctc caaaaatgat gggatagacc tgtttttgga
     8881 tcagcccatg gttggacctt cttttcatcc catgcatgaa tcagcaaatt tgtttttgga
     8941 tcgaatagat gtttataact gatttcaaac tgtctcaaaa catcttggta atctttttct
     9001 tcactaaaat tctggataaa ttcagcataa aaaggagaac ccatatataa gccatccaac
     9061 caaatttgat aaggataaat ttctttatgc caaaaagctc cttcggatgt gcgtggatgg
     9121 tttgccaact gctgggaaag gagttctgct gcttttttat atttttcttc gcctgtttct
     9181 ttgtacaata aaaagaaaag ctttcctgta tttacatggt cgatattgta ttcttcaacg
     9241 ctgtagccac ggatcgttcc atcttcttgc acgaaatagt cgatattgtc ttttatgaaa
     9301 tcaaaataaa ttttttcttg ggttcgttcc caaagtaatt ggaacccttt aagtacgacg
     9361 ccatagtcat aagaccattt tcctttatac cacttttctt catataagcg aggtgttctt
     9421 tccatgatac tttttgctaa tttctttgac cagttttcca ttatttgctc tcctttaaaa
     9481 attaaaatag gatttgggac aaataccaag aagtatttgt cccaatcgtc tatattactg
     9541 tgccgctgct ttgtatgctt cctcgtactc ttgtgtgatt cgatcgcctc cttgtgcttt
     9601 ccagtcttct acagcttttt tgaatccatc ttcgtcaagc tgtcccatga tgtattgcac
     9661 cgtagcatca atgacgattt tttcaatttc tgatcctact tcattgttcg tcggtgaatc
     9721 caatggcaca gaaggatcca ttacagcaaa ttcttcattt tctttgatca attgattaga
     9781 gaattctttt tcagggtctg tatctttgaa agagaacgtc acttcactag gacgagagct
     9841 tgataacggt tgcacctctt gttgccaaag atccatatcg ataaattcga tagaaccatc
     9901 atcattcaat tcataatgcg ttccttcgat accgccagtc atcaatttat acacatcttc
     9961 ttctaaaaga tcatcaacaa attgaagtaa tttcttcaat tgtgcttcgt cttttacttg
    10021 agatttggga aatgctagca agccgccaat accatttcct tctgaccaaa catgataacc
    10081 gccgccttca ccatctgaga tcttgtttac aggcactaat tccatatcat cttgaatacc
    10141 ttgacccatc gtcttcagat tcttgatatc caccattcca gtgtagattc cagttcttcc
    10201 ttgtgcaaat tgttcttgct gatcagtctt ttgggtaact gcaaagtctt gtgccaaata
    10261 gccattctca aatagatctc ttgaaaaatt caaagctttc atatactctt ctgtatcaaa
    10321 ttctggagtg aatttgccat tatcgtctac agcccagcca tttggtgctc caaaataaga
    10381 agtgaaaagt ttaaagctgg tataacgaat atcactaggc gcacgatcgc caaaaccaac
    10441 tgtgtcgtcc acaccatttc catcaggatc gttttctgta aattgtttag ctacttcata
    10501 aagctcatcc atggtagttg gaacatctag tcctagatta tccaaccagt cttttcgaat
    10561 gactaagcca gcacgagcgt agtctttttg tatcggtacg ccgtacaagg caccgtcgat
    10621 actcgcagct tcgattctat cttcagaaat ttgacttaaa tttttatagt cttttaaata
    10681 ttctgataca tcccagaata cgcctgattt taacgcattg cggacagaag agtttttcaa
    10741 catcgcaatt tgaagtgtaa cgatatctcc tagttcatca gaagccaatg cagttgttaa
    10801 tctttcttct tttgaagcat caggaatcca gttgaatgtc agttccgtat cggttttttc
    10861 ttcgatcaag ttcacaatcg tgtctgtagg cggtgaagca tgatgcaaaa tatttaacca
    10921 agtgatttta gcttttccat cttctgtctc tgaagaactg ttacttgaac tgctgccgca
    10981 tgcggcaagt gctagaattc ctgcaaaact aagacctaat tttacgagcg ttgctttttt
    11041 cataaatttt ctccttcact gttttaataa tatttttgtt ttcttctgtt ttttctgtat
    11101 atgagttctt ccattccaaa tggcaaaata tagaatttgc tgtccacgtg actgcggaaa
    11161 agaagagact gccaccgata aaggttaaaa cacctggcat tgtttggatg atcatcagat
    11221 aaatccctac catacttaat atcaacacta atgagtaatg aagatgtgag aatccgaata
    11281 aaaatgccat tttcattttg aaaaaatatc ctttccaatc ataatgagcc atgattggga
    11341 aaatatagag tgctgagagc acatatatga acgcagcaat atacaaagcg atcctaagat
    11401 accaattgtg tatgaaagaa agatccacaa gtaaaacaat cccgatggcg ccatatatcc
    11461 agctcatcag caaggtttca cgatagtttt ctttaaaata tgtgaagtac gttttcgtca
    11521 aaggaaacgt ttcttttgag cgcagccatt ttctaatcac tgcaaccatc gcatacgatg
    11581 caggaccgaa agtaaaaact cccaaacctg cgatgactaa accgccccac agaaaattca
    11641 aataaaccaa ctgtaaaata cgatttagcc ttccgtttat tttatctaat gactgcagca
    11701 tatctttccc tctctttcta tcttagtaaa cgccgtcttc tcctagcttt ttggcaatct
    11761 tattagctaa aattaccata aacaatccta cgaccccttt aaacaatccc attgctgtac
    11821 tgaaacttaa ctgtccattc ttcaatccag ctgtataaat ataggtgtcg aaaatttctg
    11881 caacgcttcg attcaatgaa ttcaataaca agtacatatg ctcgaaccct aagtctaatg
    11941 tctgaccgat ttttagaatc agcaaggtaa tgattactgg acgaattgcc ggcaatgtaa
    12001 catgccatgt tttgcgtaag cgtcctgctc catccatttc tgcagcttca tacaactgtg
    12061 tatcgactgc agttatcgct gaaaggtaga tgatcgtaga ccagcccaac tctttccaga
    12121 tcacttgtcc gatatatact gtgcgtgtcc attcagggga tgttagaaaa ctgatttttt
    12181 caaatcccat tgttgcaagg aattcattca atacccctcc gtcaacattc aaaaatacat
    12241 aagtaatcga aacaatgatt acccaagaca tgaagtgagg gatgtaaatg atcgtttgaa
    12301 tcccgttttt caatcgtttg ttcgttactt catttaatag taaagacaaa atgattggca
    12361 tcggaaaaaa cacgaagata tttaatccga acaagatcaa cgtatttctt aataatgtaa
    12421 agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa accgacccaa ggacttccca
    12481 ttattcctaa aaatggttga taatcttgaa aagcaataac gagtcccccc ataggaagat
    12541 acctaaaaat caagaaataa aggactcccg gcagaatcat cacatataaa aatttattcg
    12601 ttttgattcc tgcaattatt ttttttcgtc ttctgagctt ttcttctttt ttcgatagcg
    12661 tcagttgaaa tggttcttgt ttctttacac tttccataag cccctcctta gttttgtgaa
    12721 tgtttgctgc ttatgacttc atgatagata actatctgaa ttacgtatat aatcattttt
    12781 accgtaagta aaaacgctta catagcaatg atttttgtgc tatacaaaac gataaagtgt
    12841 attttttcac aaataaacta cgcatagcaa accttctatt tttttctatc tgacacttag
    12901 cataggaaat gattatatac gtaatctttc accgatgata aattgaacat atagaaacag
    12961 aaacatttaa tttattttga ggaggagcct atctatatga aaaagaaagt ctctatagga
    13021 gaaagaagtt ttacactttt caacaatacg tttctaattt tgctagcttt gatctgtatc
    13081 gttccttttt taaatatcat cgctacatct tttgcttcta cacaagaagt cgtcgcgaaa
    13141 aagtttatcc tttttcctac taccttttct ttagacgcgt atcgctacat tctttctacg
    13201 ccgaccattt ttagagcact tgctgtttcg attggtgtta caggtgtcgg aacgatcgtc
    13261 agtatgtgtg caacttcact gatggcctac ggtctttcca gaaagtattt gtttggtcga
    13321 ggtttcgtca actttcttgt tgtattttct atgttgttca gcggggggat gattccaacg
    13381 ttcttagttg ttcgttctct agggctggtc aattcttact gggccatgat cctgcctgtg
    13441 gctgttaatg caatgaatat gatcattatg agaaactttt tccaagcctt gccggatagt
    13501 ttggaagaat cagcaaaaat ggatggttgc acagattttg gtgtattttt caaaattatg
    13561 ctgccattag cgttaccgtc tattgctact atctcgcttt tttatgctgt tacttactgg
    13621 aacacttata tgactgctat tttgtatatc aacgattcat ctaaatggcc gatacagatt
    13681 cttttacgcc aaatcgtcat tgtatcaagt gggatgcaag cagaaagtag tgccgttgat
    13741 gtgattccac cagcacaaac agttaaaatg gctgttatcg ttattgcaac agtgcctatg
    13801 ctgattgttt atccttttgt tcagaaatat tttgtcaaag gagcactcgt aggttctgtc
    13861 aaagggtgat tatgtactga acgcaaaatg gtagttgtgt cagaagtggt cagcttcaag
    13921 aaataagaag gcatttacga aaattgcttc ttaaattttt gtgaaatttc ggcttatttt
    13981 tcgaagaatc tactcctgga acaccgtgta tcagaggttg tgaaagagac gttcagcctt
    14041 gagcgatgat aaaaactctc acaaacattg cgacgagtaa cccacaggag caataagaag
    14101 cgattttgaa aaatagcttc ccatatttca caaaattatg ccttattgtc gatagcctgt
    14161 ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa gacttttgtc acagttcttt
    14221 ttgcgtgctt agccttgaaa tttaactatc cataaataca aatactaggg ttattctaac
    14281 ttcttttatt gcttgaatca gtccacaaga aaaacagcct cttgattcac aaatattttt
    14341 ttcgatattc tcccggcgta atcgattcct tctttttaaa gaaacgaata aaattttgcg
    14401 gatttcgata ttgcaatctt tctgcaatct cttttacgga gaggttagtc tctttcaacc
    14461 atttttttgc tacctctaaa cgatgatttt gaacaaaatc accaaatttt tctcctgttt
    14521 cctttttaaa tacattactt aaatagttag ggttataatg caacctatca gcgatcacat
    14581 ctaaagacag atcacgatca tattctgtgt agacgatatg cataatattc tccgacaaat
    14641 ttttaaattc atgatcagtg attgcttgag agcattctgt gattggcaga actaattgtt
    14701 ctaaaacaag tttttctagt tctttgggat gataattgtt cagtgctttt acataaattt
    14761 gtttcatgtt atcaaagttt tttgaatcta ctcccaataa ctgtcctaat tgaacgagtt
    14821 cattgatcaa gcggaccgaa actacttccc gactcaaagg atttttattt tgtgcaaaca
    14881 atgcatcaat caattcatgt actaaattct tcacttcctg atttccagaa cgaatggctt
    14941 caaacaatcg attttgttct tctatcggat atctaataag tgttttttca tgagcagcag
    15001 gaacgatttc ctgataaaaa ataattgaac ttggaccagt atttacacga taatacaggg
    15061 cttcttttgc ccgatcaacc gattgcttgc tttcattcag cgtttcgaac cggttactga
    15121 tgcctatact gatagttaaa ttcaagtatt cttttacacg tttttgaatg agtcggcaat
    15181 actccatgac ttttttgctt gcttctgcat catctttatc cattcgataa atagtggctt
    15241 gcatttcttc attcaaaacg atcggcagca ttcgattttc ctttggcaca atttccgtga
    15301 tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc accgtgacta tcttctaaca
    15361 gatcgatttg aaccaaagct gtataatagc agtcattttg agaactataa ccaaattgct
    15421 gcaaccgctg atctagttct cgcctttcta ctcgatttct aaataaacta agtacaaata
    15481 gcgtttccag ctgtggcttt tgggtaacta agtgtgcact taacgcttct ttttctccta
    15541 tgatccgact gactgattga gcgaccaaat ctagttcttt cccactgaat ccttttgatt
    15601 taagattcag acgttcctgt atttgagaaa tcggacgggc aaatctgtcc gaaaataagt
    15661 aggaaagaag accaatgagt cctaccagtg ttaaagacac tgcaactagc ccgattctca
    15721 gattgcggat cattgcgcca attgcactag agtcaacttc taatctgtaa atccaattat
    15781 tataatctga tttcagtact atatacgttt tatctttttc tcttatcact ttcagctgat
    15841 tgtaatctag atccacgtcc gttaatacag ttggttgctc tttatttttt actgtctgag
    15901 tagtaaaaag gttgccaact tcgttttcaa tagaaagtat gtcttctcca ctttcaccaa
    15961 caacttgttc tattgtatgt ttctctatcg tagcaattcc caatgcatat ttttcttttt
    16021 caaacattgg caaagaaatc accatttcga ttcctttgtt tttcttttgc caaaataaat
    16081 tatttttgtt atttatgtac ttctgttgat acttttcaat ttcctcttcg gtcagttgac
    16141 tcaacgatcc attgattact ccccatttac cagccaaact gactaaatca taattattat
    16201 catccataac aatcgtttct ataaaattta gttctttcct gatttcagaa taagtggagt
    16261 aatctgtata tgtcagcgga tgcttgaata tgtccgagaa atttttggtc gaaccatacg
    16321 tagaaaacga atattcaatc gttctaattt tccattcaac gttagtttta acttgcctga
    16381 gcaaattctt tttgtctgtt aaataactcg attcgatcga ttcataggta ttctggtaaa
    16441 tgaataaact aaatcctatg acaagtacgg ctcctaataa aaacatcggt aaaaatattt
    16501 tataaaatac tgtacttttc ttcattttct tcctctcctc cttttaaaat aaaaggcttt
    16561 catttttctg taaaattgat actcatctaa ttaaatgagc attccccgct ttcaagaata
    16621 gcataattaa aaataaaaat ccaagtaatt tttaacgtgt gcaatctttt catatcaaag
    16681 atctattgac ttatcgtagt tatcgtaata tgatgaatgc gttaacaaaa agtgaaaaag
    16741 taaggaaaat gatggaatgg taacgatcaa agatgtcgct aaagcagctg gcgtttctca
    16801 tacgacagtt tctcgtgctt tgaataacag cagtttaata aaaaatgaaa caaaacaaaa
    16861 aatacgacaa ctagctaagg aaatgaatta ttcgccaaac tatagtgcaa aaggtttagt
    16921 gaatcaaaaa aaatatctaa tcggactatt tttctcaagt ttgcataaag gaacttcatc
    16981 aaatttccta gcagattcca ttgcaggcat ccattcagta ttagacacaa attacagttt
    17041 atctgttgaa agtatcgatg ttgttgattt aacagaaatc aacctgcaac gctacgatgg
    17101 actgatcgtt atgagtcaaa gtgatgatga tcagcctttc atcgactatc tcaaagaaaa
    17161 agaatttcct ttcgttgttg tcaatcgtca catcaatgat ccagcggtta tcaacgtcac
    17221 tgccgatgat gcggccggtg ttatgcaggc agtagattac ggtattggat gcggacatac
    17281 gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg acaaaagaac gggaaaaagg
    17341 ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat ccagcctatt ttttaacagg
    17401 agattacagc ttagaaagcg gcctccaaaa catggaatac ctattatcac tttctgacct
    17461 gcctactctg gttttttgcg gcaacgacga catagcgatt ggtgcattga gagctgtaca
    17521 tttagctggc ctccgtgttc cccagaacat ttctctgatc gggttcgatg attctccagt
    17581 cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag cctttgaaag aaatgtgtca
    17641 agaaggaaca aagcttttac tagatttgat cgaaggcagg acgatcgaac aatctaaaat
    17701 tgagctctcc accttgttga tccttcgtga gtcgatcaat tacttgtaaa ctacaagtaa
    17761 ttttatataa aaatgttaac gtgtgcataa cacagaaagg aagtttatat gaaaaaactg
    17821 acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag ttcttcagtt tggtgaaggc
    17881 aattttatgc gtggatttat tgattggcag attcagcagc tgaataacca acaattgttt
    17941 aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc ttggaaatat gatgaaccag
    18001 caagataatt tatatacggt cattttacaa ggactcagca atggcgaaat cgttgatact
    18061 tccgagatta tcaccgtagt agaacaaatg atcaatcctt atgatgaatg ggatgcttat
    18121 ctatctctag cagaaaatga cgacttggcc tatatcgtct ccaatacaac agaggcagga
    18181 atccaattcg tggagactga taatttaaca gatactcccc cacagagcct cccgggcaaa
    18241 ctgacagcct ttttataccg acgtttcaaa ttaaacaaag ctggctttac catcattcct
    18301 tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa tcattttacg ctacgcagaa
    18361 aaatgggatt tagaacctgc ttttgttgag tggttagaaa aagaaaatgt tttctgttgt
    18421 agtttagttg atcgcatcgt cccaggttat cctcgcgaag atgcaggtgt tttcaatcaa
    18481 cggcataact atgaagatca attgatggtc aaagcagaac cattcatgct ttgggtgatc
    18541 gaagggccta aagaattgga gaatcaacta ccattcaaac aggctggctt gaatgtcatc
    18601 atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc atttgcttaa tggaccgcat
    18661 acagcgatgg tccctcttgc tttgttggcg gaattggaaa ctgtagaaga agtaatgaaa
    18721 gatcctttat tctccgcata tgttgatcag ttattcaatc tagaattgat tcctatgctt
    18781 tctttaccta aagatgaatt agccatttat gctgaccaaa tcaaagaacg tttcttgaat
    18841 ccttttgccc atcacaaatt agaagcgatt tcattaaata gtgtttcaaa gttcagcacc
    18901 cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa atcaggtacc accgttaatc
    18961 actgtttcac tagctgcgct acttcttatg tatcgaggcg atcaagtaaa gccacatgat
    19021 gatgaaaaga cgataagcga atttactgat gcttggtctg ataatggaac agctattcct
    19081 cgattattgc aaaacgctgc gctttgggga gaagatttgt ctcaaatacc gaatgtgaca
    19141 gatacagttc aagaaatagc tgcaaagatc gatcaaaaag gtgtccggtc agtattacaa
    19201 gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa ccaacaatga aattaaatcg
    19261 acgagacagt gtggtcgtcg cattaacccc tattccaaaa caaacacaac tgacgattga
    19321 tcagcaaacg atcaccactc ttgaagatat cccgcaaggt cataagattg cattgggtga
    19381 tttgaaagct ggagacaacg tgatcaaata tggctatcca atcggtcatg tgacgacaga
    19441 cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa accaatcttt ctggtgaatt
    19501 ggattatcga tatgaaaaag atgtccatcc aagtcattac ctttttgaga accgaacatt
    19561 tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt aatgatttgt ttttagtacc
    19621 taccgttggg tgtgtgaatg gcattgctga actgatcgtc aaacagttca aggaaaaaca
    19681 tccagatctg ggacagtttg accatattac gattttaaag catccttatg gctgttcgca
    19741 gcttggaaaa gatcatcaaa atactcgaga aattttagcg gacgcagtaa atcatcccaa
    19801 tgccggagga gttttagtat ttggattagg ttgtgagaac aatacagttc ctgaattcaa
    19861 aaaaatttta ggtgaatatg atgaagaacg agtgaaattt ctagtggctc aagatgttta
    19921 cgatgagatc gaacaaggtg tggctctatt agaagagctt ttaacagcgg cggaaaatga
    19981 ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt ttgaaatgcg gcggttctga
    20041 tggtctatcg ggaataactg ctaatccttt acttggtgca ttttctgact acttgattgc
    20101 tcaaggaggc agcacgatct taacagaagt gccagaaatg tttggcgcag aacaagtatt
    20161 gatggcacga gcggaaaatg aagaagtttt tgactcaatc gttgacttga tcaatgactt
    20221 caaacagtat ttcttgtctt acggggaacc agtttacgaa aacccatctc ctggtaataa
    20281 agcaggtggg atcactacat tagaagataa gtctttaggc tgcacgcaaa aatcaggaag
    20341 ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc agaaaaccag ggcttagttt
    20401 attgcaagct cccgggaatg atcttgtagc tgcctctgct cttgcttcat ccgattgcca
    20461 gttggtcttg tttaccacag gaagagggac tccgtttggc tcatatgtcc caacggtcaa
    20521 ggtatctacg aatacaactc tttttgaacg caaaggtcat tggatggatt ttaatgcagg
    20581 cgtcttatta gaacaaccaa tggaagtaat cttagaatcg tttgttcaaa aaatcatcgc
    20641 tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac gttcgagaaa ttgccatttt
    20701 taaaaacgga gtaacacttt gattttcata aaacagtata aaaagaaaga aggaagattt
    20761 atgtttttat ctgatgattt tttattaaaa gacgattggg cgaaaaagct ttaccattcc
    20821 tacgcaaaaa acatgcccat tattgattat cactgtcact tagatccaaa agaaatttac
    20881 gaaaacaaca atttcagcaa tctgacagaa gcttggttag gaggcgatca ttacaaatgg
    20941 cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca caggaaatgc ttctgatttt
    21001 gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta tcggaaatcc gctttacagt
    21061 tggtcacatc ttgaattgaa gcgtttcttc ggtattgact tgttgataaa tgaagaaaat
    21121 gcagagaaaa tttggacaca agcaaatgaa attttggcag ctcctgattt tcgccgtcgg
    21181 gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa cagatgatcc cgcagacgac
    21241 ttgcactatc atcagttgct tgctcatgaa gaaccagatt tgaaggttct accttcattc
    21301 cgtccagata aagcactcaa tattgaaaaa gaaggattta atgcatgggt cacactactg
    21361 gaagaagcag ctgaccaaac gatcaccacc tatcaagaac tgatagaagt cttagggcag
    21421 cggatagaat acttccatca acatggatgt cgtgtttccg accacgcttt agacatctta
    21481 cgctataaag aagcggatga aacagtatta gaagagattt ttcaaaaagc acgagctaac
    21541 aaactattat cttcagatga aatcgatgct tatcgtacag aaacactgat acatttgacc
    21601 cacttctatc attctcataa ttggacgatg cagctgcaca tccatgctta tcgtaactgt
    21661 aatacacaaa tgttggaaaa attagggccc gataccggct atgatggaat gaatgatctt
    21721 tctttgacta ttcctctgca aaaattattg aaccgtgcag aagaaacgga tcagttgcca
    21781 aagacgatcc tttattcatt gaatccgaat gactatcctg cgattttagc tttgatgggc
    21841 tgtttccaaa aagaacagaa tggaaaactg cagttaggtt ctggatggtg gtacaacgat
    21901 actcgagctg gtatgcgcga tcaattgaca caatttgctg atggtagtgc tttggggaat
    21961 tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct acactagaca tgaatatttc
    22021 cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc gaggagaagc acctgaagat
    22081 gaacaacttt taggaagtct agttcaagcg attagttata caaatgctaa aaattacttt
    22141 ggcttttttg cctaattgat taatagtaac agtaacgaat caaaaacagg atagtgaaat
    22201 aacggaatcc gtttatccac gttttagaga agaagacgga ccaaatttgc tccgtcttct
    22261 tctcttgctg ttctgcttca ttgcctataa acctttatga aagaagagat aacatgccta
    22321 ctatccatat tgccggtgat tctactgcgg cagaaaaaga tgaaaacaag cgtcccgaga
    22381 caggttgggg agaaaaaatc tctgcttatt tttcacccga gatccagatc aataaccaag
    22441 caaagaatgg acgcagcacc aaatcattta tccaggaagg aaggttagcc cgtctagctt
    22501 cacagtttca gccgaatgat ttcttatttg tacaatttgg acataacgac cagaaaatcc
    22561 aagaagaaag aggtacgctt cccttcggta gctatttaaa aaatctgcag atattttttg
    22621 aatgcgcaaa aaaagcgaac gtccaaatgg tcttactgac atctgtgacg cgacgagatt
    22681 acttagaaaa cggaacattg aatccagata tacttggaga ctatccaaaa gcgatgcgtg
    22741 catttgctga gaaacaccat attcctcttt tagatgtgtt ctcaagatca caagaattgt
    22801 tccagacctt ctcaaaagaa gagacaaaaa aattctacct gcatcttata cctgatactt
    22861 gtaaaaatta cccagaagga ctaaaagaca atacccactt tagtccagaa ggcgctgata
    22921 aagttgcccg aataattgtt gaattgataa aagaatcccg tctgcctctt gctaattatt
    22981 tacaaaaagg agtttgagat gtttccatta aatttaggca tccgtgccca cgatttagat
    23041 gttcgaaatc gtgaagaaat cgttcaaaaa gtatcacagc ttcacttgtc ccatatccaa
    23101 tttgcaccac aaaaagcatt tcctgaacaa cttaaattaa ctgacatgac acttgggagt
    23161 gctgcctatt ttggtgaata cttccaaaaa aacaatatcg aactttccat tttaggatgc
    23221 tatatcaacc tatccagcaa aaatcaagaa gttcgtgcac aagcattggc aactttcaaa
    23281 cagcaaattg ctctgtgctc tgcctatcaa gccaagatga ttgctacgga aaccggtagt
    23341 gtctctgtcg gttacacaaa agaaaatttt actgaagaag cttatcagat cgccagaaat
    23401 tctatcattg aattagtcgc tcatgcagaa aattttggcg taactgttgc tgttgaagca
    23461 gggatcaatc atccaatcta taactatcag ttagctaaac gtttaatcga tgaagtagct
    23521 tcaccaaatt taaaaatcat tttagattgt gcgaatttga tgcataaaga aaatcatgct
    23581 gatcaggaaa aagtgatccg aggtgccttg gacaatttac atggttatat tacagcgctt
    23641 catctcaaag actatatcat ggaaaatgga aagatccgca tggtacctgt tggcaagggc
    23701 tgtttagatt tccgcccggt actgcattat atcaaatatg agcgtcctct aatgtacgcc
    23761 actcttgaag ctactcctga gatttatgtt cctgaagcaa tagaacactt gcagtttatt
    23821 taccaagatt gttaaggaga agattatgga ggaacgaata cagaaaagtt atcaaaaata
    23881 tcatgcaaat gcttttcaga cattgctgca tctttatcag ggaaattatt ggcgttttgc
    23941 catgtcagga ctctttttta ttatcaaaca tcttccatct tggttaatgc cgatcgctat
    24001 tgccaatgta atcaatgcag cttcatcgaa agattcttct cagttgcagg ccatttattt
    24061 caacgctttt ttgatggctt gtctgattgg tcaaaatata ttgacgaatt attttcatgt
    24121 aaaataccat agcacttcga tccgtcaagt agaagctgga ctaagagcga cccttatcaa
    24181 gaaaatccaa gaactatcta ttccttatca aaaagaattg caatctggac gaatccagtc
    24241 aaagatcatc cgtgatgtag aagctatcca aacgttgtcc tctcaagtat ttgtcagcgg
    24301 attaaatatc gctgtcaatt tattcgtagc tttaggtatc acgctttata aaagtcctgt
    24361 tgttttcatg tttttcttat tgactgttcc tctcgccgca ttcgttactc tctttttccg
    24421 taaaaggatc agacagtcca actcagactt caggcaagaa atggaagaga cttctgcaaa
    24481 agtgatcgaa gtgatcgaat tagcaccgat tgctcgagct catgggttag aaaaacacga
    24541 aacaactcgc ttaaataagc attttactca aatctatgaa aaaggattcc acttagatat
    24601 cctgcaatct gttttcggat cagtcagctg ggctagtttt cagatatttc agctgatttg
    24661 tttgattttc actgggatat tagctttaca agggcgcatc caacccggcg atgtcgtgat
    24721 gtatcagacg tatttcacga cgatcgtcaa ttccgtttct ggaatgatca cgctgattcc
    24781 tacgatttca aaaggcatgg aatctatcac ttctattggc gaactgttga ttgctggcga
    24841 tgtagaaact tatcacggta agaaaaaaat ccaacatttg gatggaaatt ttcgttttga
    24901 ggatgtttct tttctttatc cggactcact caaaccgatt atcgatcatt tttctctaga
    24961 ggtgaaagct ggagaaacaa ttgcttttgt tgggccatct ggctctggaa aatctacact
    25021 ccttaactta ttgattggat ttatccagcc gacttcagga aaaatttttc tagatgatca
    25081 gcctttggat gaactggata tgcgaagtgt gcgaaattat ttagccgttg ttcctcaaac
    25141 cactttgttg ttctccgctt cgatcaaaga gaacatcact tatggcttga aaaacgtatc
    25201 aaaagaacgt ttagatgaag tcatcgaagc tgcgcaactt tcttcactga ttgaccagct
    25261 tcctgatggt ctggatacct tagtaggcga acatggcaac aagctgtctg gtggccaaaa
    25321 gcaacgtatc tccattgcac gagcgttgat acgtcagcca aaaatcattt tgctagatga
    25381 agcaacctca gctttagata accagtcaga gaaaaaaatc cagcaagccc tgaattacct
    25441 gacagaaaca cccactactt ttattgtggc tcatcggctt tcaacgatca aagaggcaga
    25501 taaaatcgtt gtgatcgatg aaggaaaaat cgttgaaata ggtacttacg aaaaactgtt
    25561 agctcaaaaa ggctatttct accgcttgaa aaatgaatga caaaaaatag gagctatgac
    25621 aagacttttg tcgtagctcc tatttctgaa taaacggtgt tctcctgcca actcctcggt
    25681 cacaagtcac aatagtcatc aaacatgaga agctgttttt tgaaattgtt ccttgtaact
    25741 cagagttaaa cggtaggctc acgacctctt ggtaaatggt gttcaagaga cagcctatcg
    25801 gcaataagaa gcaattttgt catcgctcaa ggccgaacgt ctctttcaca ccctctgttg
    25861 tttcataaaa tagttttatt cgctgtaaag gtcgcgaact ggtgtcatga tgatacggtt
    25921 caagtcattg atgattgtac tgaagccttg ctctttttgc atcaagtcat tgattacttc
    25981 ttctgattgg attttcacag ccatttcttg tgctttcatt gcatcttcgt cgctgaactc
    26041 ttgtccactc atttgttttt cttgtaattc gatttgcatc gcttggaagt ctttgaacag
    26101 tttgtgtgct tcttcgtttg cttgtacttt ctcgtacgct tctttcaacg ctttgaattc
    26161 tggtaattca cggatctcac gttcgatttg attcgcgcta tcgtagatgt ttgacatgtt
    26221 atgccctctt ttcttttagt agttactctc tattgtaaca tggggttatt cgttttcaaa
    26281 ggggctactc tccttgatat ttccgccaga ggtctttagc cgcttcacca attccctgaa
    26341 cgccttcttt caccatatcg ccgaagtttt tcagcccttc ttttgcttgt tcaccatatt
    26401 gattcaagtt atcttgccaa tttccagact ctccattaga atttcctgtg tttccaactt
    26461 gatctgcagc taccacttgt cctcctgtgg cataggcatc tgctacacta aatcctgttt
    26521 ttgaagtata cggaaggatg ccttctgccg ctgatttgaa gactttcccg acaacatttc
    26581 cacttgtccc ttccaaatag tgcagcttgc tggattcttc gtaacccatc catgtcgaaa
    26641 tcactacgtc tggcgtgtaa ccgatcatcc attggtcatt tgcttttgtg gcatcgaaat
    26701 tagtttctgt ggttcctgtt ttccctgcta tcgtataacc tgcaggattc gcagcagccg
    26761 ctgttccatt tgaaaatgta ccaagaagca tgctggtcat ttcatcggct gtttcctttg
    26821 atatgacctg ttttggtttt ttgtttgtgt tatcgacgat caccgctcca gtcgaatcaa
    26881 cgattttcgt gataagatgc ggcgtataca tctgaccatc attggcaaat gcactgtaag
    26941 cacccgccat gatcaatgga gatactcctt tttccagtcc accaagggct aagccataat
    27001 acttatcaga ttttgctaaa ggcaggccga attcttctgc tttatcatat cctttttgta
    27061 aaccgatctc atgcaaaagc catacagtag gcaaattcaa actttgtgca acagcttgat
    27121 acatcggtac ttctccttgg tatgtcccgt caaaattatg cgcatcgtaa taactttgcg
    27181 gttcgtcttt caagatactg gaaggcttgt atccagcttc cagtgctggt gtatatacgc
    27241 ttaatggttt gatcgttgat cctggtgaac gacgcatctg tgtagcaaag ctgaatcctc
    27301 taaagacatg atctcctctt cgtcctacta gtgcttgtac accacctgta ttcggatcta
    27361 aagctactga agcagattgg accatggcac catctgaagc atttgctggg aataatgcac
    27421 tgttttgata agtagcttct aattgctttt gataatcctg atctaaagct gtatagattt
    27481 tatagccttt attcatgaca tcttcttcat caagaccata ctcgttaact gcttcatcga
    27541 tcactgcatc aaaataataa ggataacgat aaccagaatc tgaattatcg tatgtgtcat
    27601 taagatacgc gctgatatct gtctggcttt ctgcttgttc ttgttctttt gtgatttttc
    27661 cgttttcttc cattacactt agtactgtgt ttttccgatc attcgcattt tttgggtgat
    27721 cgatcggatt ataaatacca ggacctttca gcattcctgc caaagtagca gcctcagaca
    27781 gcgtaacatt cgttgcatct actccaaaat actttctgga agcgtcttgg acaccccata
    27841 ccccgttgcc aaaataggca ttgttaagat acatggtcaa gatttcttct ttgctgtact
    27901 tcttttcgat ctcgattgcc aaaaataatt ccttcgcttt tcgatcaaac gtttggtcta
    27961 atgtcagata cgcatttttg gcaagctgct gagtgatcgt acttcctccg ccccctgatg
    28021 ttccgccaga caagaccatt cggactgctg cacgggcaat ccctttgata tcaaacccgt
    28081 gatgctcgta aaaattccta tcttctgtcg agattactgc atcttggata tagggagaaa
    28141 tcgcgtttaa ttctacatat gttccttttt gggcgtacag tgtgccagct tcttcgtttt
    28201 tcttatcata aatgactgtc gattggctta atcctgattt taacgtttcg acatttgctt
    28261 gttttgctaa aataaagagg tagatacttg tcactaagac acagatcaaa ccaatcagca
    28321 aaatcaattt gttcacatga tattttttcc agatgcgctt tcgccatcga tggaattgtc
    28381 cgagatatgg tctgatccaa cgccaaaaac tgatcaaagc agcttttatc ttttctaaaa
    28441 tgctttgaaa atccatttgc ttcttctcct gtctatttga ttcaatcgtc ttattttacc
    28501 atagtgaaag agtgttttct tcctgcttaa gttgtagttc aatatgtttt tttacgttag
    28561 tgcaagtaga gtaacataag aatgaacaga aaaatgtaag ttttttctta aatatgtttt
    28621 aaaaatggta caattagtct gcgattgcac tgaaaggagt acacttgtga aaatcacgat
    28681 cactttacct atgaaccatc ctgaaacaac aattcgcgac ttgcttgaaa aggaatggtt
    28741 agttcctaga aaggttcgac actttttgcg tatacgtaaa aacgtttgga tcaatcaaca
    28801 acctgctttg ttccatcatg aagtaaaggg caatgaccag atcacattgt tattcgaaga
    28861 aacagattat aattatcaag aagttcaatt aggagatgcg aagcacatcc gtgttttata
    28921 tgaagatgag catttgatta tagtggataa gcctgcagga atgaagactc atccaaatga
    28981 accgacagaa aacagtacct tactcaatca tttagctgct tatctcgaca ctaaacagca
    29041 acgaccttac gttgtccacc gcttggacaa agaaacaagc ggtgccgttt tatttgcaaa
    29101 aaatccattt gtcttgccga tacttggaag aatgttggaa caaaaaatga tctatcggag
    29161 atatcaagcc atcgtatggg gagaaattgc caaagatatg accatcacca ataaaattgg
    29221 acgagatcgc catgatcgga gaaaaagggt cgtagatgat aaaaatggtc agagcgcctt
    29281 gacacatgtc actgtcaaca aagtactttc tgaacaaaca caagtctatt gtgttttgga
    29341 aaccggacgt acacaccaga tcagagttca tttagctcat aagggacatc cgatcgttgg
    29401 agaccctctc taccaaaata gaacagctga acgactgatg ctccatgcat tagaactgca
    29461 catgatccat ccatttactc aagaaaaaat agtcggtaaa gctttacctg gattatggga
    29521 atagaaataa aaaagaacag agtcttatca gcagataatg ataagactct gttctttttg
    29581 tgtaatttgt ctgcttactt ttgatgttcg tgttctgcta acagcaaact accgataatc
    29641 ccactttcat tcggcagtcc ccaacgaacg atatattcac ttacttgagg tgtttccata
    29701 taaccaccca tcaattcaac aaattgctga cggatttttt gaaggagatg atcctgattc
    29761 attaccccac cgcctaaaat aatcttttca ggtgctaacg tcaacgtata gttgatcagt
    29821 gcttgagcaa tataataagc ctgaatagcc caaacaggat gatcttgcgg cagatcttgc
    29881 cctttgatcc ctgtgcgtgc ctcaatagac ggtcctgctg ctaatccttc taaacaatct
    29941 ttatgataag gacaagtacc ttcataatgg tcatctggat ggcgtttcac ccaaatatgt
    30001 cccatttcag gatgtgcgat ccctgaaaaa atttcttcat tgatcacgac acctgcaccg
    30061 attcctgttc ccacggtaag gtaaatacaa ctattttttc cttgtgctgc tccttgcttc
    30121 aattccccat atgcagcagc attgacatcc gttgtccaag cagcaggaat attgtagcgt
    30181 tccttgattg tgccaagaaa atcgaattgt gcccaccctt ttttgggagt tgccaaaacg
    30241 tatccgtaat tatcttttgt tttgtcgata ccaatcgggc cgaatgagcc aatcccgatt
    30301 cccttcaatt cataacgatc aaaaaattca aatacctgat ccaatgtttc ttctggtgtt
    30361 gtggtaggga tacttaattt
//
LOCUS       DAZKOB010000021        28786 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000021 DAZKOB010000000
VERSION     DAZKOB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.021"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1222)
                     /locus_tag="ACG8M6_000988"
     CDS             complement(56..1222)
                     /locus_tag="ACG8M6_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0456675.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(1234..1587)
                     /locus_tag="ACG8M6_000989"
     CDS             complement(1234..1587)
                     /locus_tag="ACG8M6_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0456676.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            1715..2737
                     /locus_tag="ACG8M6_000990"
     CDS             1715..2737
                     /locus_tag="ACG8M6_000990"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0456677.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            2756..2917
                     /locus_tag="ACG8M6_000991"
     CDS             2756..2917
                     /locus_tag="ACG8M6_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456678.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(3176..3610)
                     /locus_tag="ACG8M6_000992"
     CDS             complement(3176..3610)
                     /locus_tag="ACG8M6_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456679.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(3720..5579)
                     /locus_tag="ACG8M6_000993"
     CDS             complement(3720..5579)
                     /locus_tag="ACG8M6_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0456680.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            5580..5720
                     /locus_tag="ACG8M6_000994"
     CDS             5580..5720
                     /locus_tag="ACG8M6_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0456681.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(5697..6857)
                     /locus_tag="ACG8M6_000995"
     CDS             complement(5697..6857)
                     /locus_tag="ACG8M6_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0456682.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(7164..8588)
                     /gene="araA"
                     /locus_tag="ACG8M6_000996"
     CDS             complement(7164..8588)
                     /gene="araA"
                     /locus_tag="ACG8M6_000996"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0456683.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(8604..9302)
                     /locus_tag="ACG8M6_000997"
     CDS             complement(8604..9302)
                     /locus_tag="ACG8M6_000997"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0456684.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(9259..10920)
                     /locus_tag="ACG8M6_000998"
     CDS             complement(9259..10920)
                     /locus_tag="ACG8M6_000998"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0456685.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            11138..12538
                     /locus_tag="ACG8M6_000999"
     CDS             11138..12538
                     /locus_tag="ACG8M6_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0456686.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(12790..13869)
                     /locus_tag="ACG8M6_001000"
     CDS             complement(12790..13869)
                     /locus_tag="ACG8M6_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0456687.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(13955..14914)
                     /locus_tag="ACG8M6_001001"
     CDS             complement(13955..14914)
                     /locus_tag="ACG8M6_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0456688.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(14926..16437)
                     /locus_tag="ACG8M6_001002"
     CDS             complement(14926..16437)
                     /locus_tag="ACG8M6_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0456689.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(16459..17022)
                     /locus_tag="ACG8M6_001003"
     CDS             complement(16459..17022)
                     /locus_tag="ACG8M6_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0456690.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(17028..17891)
                     /locus_tag="ACG8M6_001004"
     CDS             complement(17028..17891)
                     /locus_tag="ACG8M6_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456691.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(17905..18801)
                     /locus_tag="ACG8M6_001005"
     CDS             complement(17905..18801)
                     /locus_tag="ACG8M6_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456692.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(18976..20265)
                     /locus_tag="ACG8M6_001006"
     CDS             complement(18976..20265)
                     /locus_tag="ACG8M6_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0456693.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(20237..21754)
                     /locus_tag="ACG8M6_001007"
     CDS             complement(20237..21754)
                     /locus_tag="ACG8M6_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0456694.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            21952..22842
                     /locus_tag="ACG8M6_001008"
     CDS             21952..22842
                     /locus_tag="ACG8M6_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0456695.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(22915..23739)
                     /gene="nadE"
                     /locus_tag="ACG8M6_001009"
     CDS             complement(22915..23739)
                     /gene="nadE"
                     /locus_tag="ACG8M6_001009"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0456696.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(23751..25223)
                     /locus_tag="ACG8M6_001010"
     CDS             complement(23751..25223)
                     /locus_tag="ACG8M6_001010"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0456697.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(25860..26222)
                     /locus_tag="ACG8M6_001011"
     CDS             complement(25860..26222)
                     /locus_tag="ACG8M6_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0456698.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            26445..27197
                     /locus_tag="ACG8M6_001012"
     CDS             26445..27197
                     /locus_tag="ACG8M6_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0456699.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(27337..28260)
                     /locus_tag="ACG8M6_001013"
     CDS             complement(27337..28260)
                     /locus_tag="ACG8M6_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0456700.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(28275..28547)
                     /locus_tag="ACG8M6_001014"
     CDS             complement(28275..28547)
                     /locus_tag="ACG8M6_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456701.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         8522 a         6001 c         4890 g         9373 t
ORIGIN      
        1 aaaatcttca aagcagcatt tgtaccttgg atattttccc atctgtctct ttttgttatt
       61 ctttctgctt tttcaacgga ttttcttcat aaagagaaga gattgttgtg taccattcaa
      121 aaacatgagt aatcactact ttagctgctg cataaacagg aatccccaag atcacaccga
      181 tgattccgaa caatttccca gaagtcagca gaactaataa aatcgttact ggatggattg
      241 ctaactgatt ccctagtact aatggagaga tcaagcgtcc ttctattgtc tgctctactg
      301 caaaaacgat cagtactttg atgatcatga atggacctgc tacaatggct aaaaagatag
      361 ccggaatcat cgctaaaaat gatcctaagt aagggatcag gttcaaaaaa cccgcaataa
      421 tccccaacgt gacagcatac tctaaaccaa tgaccgcaaa accaatcata aacatgattg
      481 ccacagcaaa agccacagtt agttgtcctc ttatataaga agaaacttga ctattcattt
      541 cactcagcac attcaacgtc ggtttttgca tgcgtgtcgg taaaaatttg acaaaataag
      601 gtgctaattg tttaccgtct ttcaatagat aaaataagat aaacggcatc gtcagcagag
      661 ccactaaaat agtcgctacc gcgccgacaa aacttccaag actctgaact gtactcttag
      721 atatatcttg gatcatatcg cctgccgaat tcatgaactt atcacctgct gtttccaact
      781 gttcgcgaaa ttggttgaac agcgggtccc gcaaaatctc ggttaattta ttatcaatcg
      841 tatctacata ttgagggaag ttactgatga agctcactgt ttgttcctga atcttaggga
      901 tgatcactac gctcccccat gcgattagtg caacaaccaa aacaaacagc ccaatgatac
      961 tgtaaagcct tggtactttt cgcttttcaa agtaatcaac taccggattc atcaagtaat
     1021 acaagattcc tgctaaaatg ataggcagac ctacgattgc taaaaattgc cagattggtg
     1081 caaacaaata tgaaactttt gtaaataaaa agacgatcag caatattaac aatacaacaa
     1141 gtaaggctgt gactacttga ttatttaaaa accagcgcca aaaccacgat accggtctgt
     1201 tcttcttatt ttttccttcc ataattcctc ctgttagtta taaaaaattt ggcttcccaa
     1261 accaatagca ggcagtctat gcggatgaag atagattcca tttgcaattt gtccttcttc
     1321 tggacaagca tcaaaaatca atgatacgtg agctactgtc tctaaattcc cattggccat
     1381 cggaccgaca taatcgatcg tatatacctg atcatcaaaa gcaattgttc cgcctgcttt
     1441 caaagaaaaa ggttcgtctt ttgatacttc ttgaatcacc gaatattttc gtaaggcagc
     1501 tgttgctgtt ttatcgaaca aaatcagcat gggttcttcc ttgtttattg cttctgtccc
     1561 aatttctgta atctttgcct gaatcattgc actctctcct ttaattgctt tcaaaaacag
     1621 tataacacat tctcattctt tagccgtgtg gaataattat gtgatagaat aaaaacgaaa
     1681 actatttcat tacaattagg aggaatcagc tcatatgatc gaaaagataa ttttaggaac
     1741 ctatacgcgt cgcgaaagta aaggaatcta tacgatcgac cttgatacag aaaaagaaga
     1801 attagccaac ctttcattag ctgttgaaga aaatagccct acctatgtag caaaaagtaa
     1861 agctggcaat ctttacacag ttactagtgt ggatggatta ggtggtgccg gtgcgtacga
     1921 taaggatttt cattttttga atgcggtaac agagtctggc gctcctttgt gttatgtagc
     1981 ggttgatgaa gcacgtcaat tactatacgg ggcgaactat cataaaggtg aagtaaatgt
     2041 ctaccatata ttaggagatg gcgaactgaa agcagcagat tcaatattcc atcaagaagc
     2101 gacaggtcct cataaaaacc aagatcatgc gcatgtccac tatacggatc tgactcctga
     2161 ccaacgatta gtcgtatgtg atcttggtac agatcgtgtg tatacttatg atgtgtccga
     2221 aaacggcaaa ttgaatcttg taactacctt cactgcagag ccaggaacag gccctcgcca
     2281 tcttgtcttt catccaaatg gacaattcgc ttatttattt ggtgaattag acagcacagt
     2341 ccgcgtgctt tcttataata aagaaacagg tgcttttgaa gaattacaga aaatatcgac
     2401 cttgccagaa gaattcgacg gtgaaaatgg aggggcagct atccgcattt caaatgatgg
     2461 caagttcctt tatgcttcta accgaggaca taactcgatt gctgtattcg cagtttttga
     2521 agatggttcc cacattcaaa acaagcaaat catctcaaca gaaggggatt ttccccgcga
     2581 tttcgcacta gatccaagca ataattttgt cgtgtgtgcc aatcagaaca ccgataatct
     2641 gacattatat cgccgtcatc ctgaaacagg attactggaa ttactgaaaa aagatgtcta
     2701 tgtaccagaa tgtgtctgtg tttattttga tcgttagtaa gaaaaggaga atgaaatgaa
     2761 tcagcaagac gtattagaca gactacggga agaactaaat attcctttct ttgatgggaa
     2821 aatcgaagat aaagagtata gcgaagagga atatcagaaa ctcaaagccg atttacagaa
     2881 ctactttgaa caatatgtac agaatgtaga aaactaatca gctttccata ctatctgaaa
     2941 agaacatcag gacaagactt ttccgtctat cctgatgttc ttttctacaa ataaaaaacc
     3001 gccgagatgg ttagactcga cggaaaagga gtatttactc gattttgagt aagtacaagg
     3061 ttgttgttgg ttataggtat gtcattatca taaaggattt tgatcactct atcataaagg
     3121 taatattaca tttatattag ataactctag tttttattgt tatcaaagat ttcttttatt
     3181 ttttcaacaa caaattagtc attttttgga tggcagctgt caattggtct gtcgcttggt
     3241 cttgttttgc ttcagatgta ttgaatagat cttcatctac ccctgattca agatcgatac
     3301 tttggatcat ttcttcacag cctgctacat atgaaagata agctttttca aaatttttat
     3361 gaatgcccat tgcttttgct ggtggacgca aagtactgac tttcttaagc attgcccgat
     3421 atttttttgt tccttcttca aataacagga tgatctcttc aagagtcgat ttgtccagtt
     3481 cacttgtttt tccttcatca attgcgtttc tgatcacttc atatttcgga tgcattcctt
     3541 ctccgattgc ttctgtttcc ttgacgattt gatcgatgga ctgcacataa tagccaatat
     3601 tcggtaacat gttcgttttc ctactttctc cattgttgtt attatttatt ctacttgttt
     3661 ttatcctcaa atgacaagta cttgaaaaaa tttaatggtt tctatacagc tcgccattct
     3721 tattcgtgct caaagaaacc atctgctaaa ctatattgtt tccctgcgac aatgtcatat
     3781 tggatcaatt gatattcgtc gtaaatctct tgttgcgagc gattgaattg cgcttcttta
     3841 taccactgtc cgttttgata gtacagttct cgttcaaacg ccggaagttc ctgttctaat
     3901 gataaaagca gctgataaaa gccatttgtt gtctgattcg tctgttccat tagattcgct
     3961 gcaaaataaa acggactggt gattgcatcg tgagtcgttt gcttttctaa ttctccttta
     4021 ctgtcgaaca taagaaattg tgtttcatgc aatgtttgtt tgtcattttt tgcttggatc
     4081 gtatctgaat agatccctgg aagatgatcc ccccaaaaaa caactaaagt ccttctagaa
     4141 agattcttca attcttccgt gaactgcttc agtgccgtgc tgctatagga aatatcttgc
     4201 agataattct ctaagtcaag tgtcccactt ccatctgaag tttttgcagg atagtctaga
     4261 ttatcatact ttccatcgta gggcatatgt gtctgcatcg tgactaaatg aatgaactga
     4321 ggtgtttttt cttccttgag aagtgtcatt acttctttgt aagctgactc atcagaaata
     4381 tatggattat tctctatcgt atccgtatag gtcatcgtac gctcgctgat gaactggtca
     4441 aatcctaatg tctgataaac atcctctctt ttatacatcg aagtattgta tggatggatc
     4501 gctgtcgtct ggtatgattg tgcgtttaac gctgaaacga tagaaggaag ctgatccatt
     4561 tttggtacaa gcatcgtgta aggggtggtg agttgcgcat taaataacgc catagaaaaa
     4621 ccagtcaatg cttcaaattc gatatttgct gttccaccgc catagttttg agaaagcata
     4681 ttgccactat atgtttgatc agctacttca taataatctg ccaatggctc accagatact
     4741 tctacgccgt ttaatcgact aggatctgag aaactttcac tcatcacgaa cacgatgttc
     4801 ggcgactctt cctctacagt tttatttttt tcatcggcta gcttctggta tttttcagtg
     4861 atttctttga tcttttcttt cgaatatcct tcaggttcat ccattggttc taccttcaga
     4921 ttgtacaaaa acccaccgat aaaaccagtg ttatagtagt tcattttctg gctgtaaggt
     4981 atccatagtg ccgttttatt atatgctttt cgcaacaaat tatcgggatt gttgaaatta
     5041 ctgatatacc ctagcagtcc aattgccaca agcagtgtcg ttaatcggat caattggaat
     5101 cgccttcctt ttttcaggct tcgaaacatg taccagcaaa acaatcctat cacggttcct
     5161 gcggcagcta agatcactgt aaacagcata gtgccggtca tatctttcag caatccgatt
     5221 tcagtgatca tcttcaaatc gtctggataa atcggttctt gccgataact cattttcata
     5281 taatttgcaa aacctaatac accgatggct acgacatacg taagggaacc taaagggaca
     5341 gatcctgcta gtgcaatcag aaaaataaga ataatcagca agacgagaca ggctaagaag
     5401 aatttttccg tatgccaaga aaaagcaaat ttcaacgcaa ggtctacgga tagatcattt
     5461 tgacaccact gcaaaaataa attactaaaa acaacaagaa aaaagatact gatgattgct
     5521 gctgttcctt ttaaaatctt tttctgtttg gtcgaaagtc tgcttatata tcttttcaaa
     5581 tgcttgctcc ttcatttcta tcaataaaag aatgttgctt ctcttatttt agttgtacga
     5641 aattcgaaag taaagaacgg aaagaagaat taatgaaatc caaatattct ctttctttat
     5701 ggcctttctg gtttctttaa taagcggacc tttcgtactc cagaaatttt ttctagctgt
     5761 tgtttcactt gattgagctg ttcaaaatca gtactgtcta tgtcaatcaa tatgtagaca
     5821 tgctcattct tatggttacg cgatatatct gcgatcgcta gctcgttctc attcagcaga
     5881 gaaaatatct ctgctaatat attggttttc ttttgataaa atatagtgaa tcgaaacggc
     5941 gcttggaaaa acattcgtgc ggatggataa ttgaccgctt ctctaaccgt accgaacaaa
     6001 agataatctt ttaaggcttc aactgccaaa cgtccgctgt ctgccaaagc ttcctgagtc
     6061 gatcccccta gatgaggcaa cataagtata cggtcatttt ctaagaactc ttcttctgga
     6121 aaatcagtca agtatttagc cagatgttgt ttttccaatg cttgcataac agcatattta
     6181 tcaacaattt cacttcttcc tacattgata agtacagcat cttttttcat tttttctata
     6241 ttcgcctgat cgatcaatcc ttttgtatct tctgtcaacg gcagcatgac tacgatgtaa
     6301 tccgaggtag aaagaactgt ttctaaatct gcttgccgaa cataatccaa ctgggcatcg
     6361 cggatagaat aacccaagac atccatccct aagctatagc aacttagcgc tacttttgtg
     6421 cctatcgctc caagaccaag aagaccgatg gttttgcctt gcaattctcg tcccacataa
     6481 gcacttcttt tgttttctgc ctgttctaaa atattaggac ctgttagtgt ttgtaccata
     6541 cgacttgctt cgattatcgg acgggaagat aagagcaaac agcacaaaac aagctctttt
     6601 actgcattag catttacacc tggtgtattc atgacgatcg tcccgttttc agaagctttt
     6661 tcaacgttga tcgtgttcac cccaacacca gctcttgaaa ttgctagcaa atctggtgta
     6721 agccattcat ctttcacttt cgtactacga ataactattc cttcggctgg caaatcatct
     6781 attattttga aatattcccc ttgtaacttt tctatctctt gttctggaat agcatcaatc
     6841 aaatgaacgt gatacataaa accccctcct tactaacaag tatctcgaaa tttctgcaat
     6901 tagacaactg tttaccattt gacttcctag aagcaaaaag agcagttaga gcagagtagg
     6961 ctgagtgata aaaataaggc tatgacaagt tactgccata agccttattt tcgagagcaa
     7021 aatattgagg gtgagatatc ttgataaatg gtgttccgtt agctgacatt gataataaga
     7081 ctaaatttca aaaaagttga aaagcaaatt tcgtcaatct cttcttattt cttaaaacta
     7141 agtggctttt tcacaacctc tttttatcta ataaccactg tttccaactc aactaatttt
     7201 gcccacgttt caatttgatc tgttgttaga tttagcgaga caacggtatg atgaccgcca
     7261 cctgcctgaa tccatgaatg gacaccttca tggaaattcg gtttagtttt ccataacaca
     7321 cgtgcaacag gtaaattagg tgcttcagtc gttggttcaa aggcttctac ttcattgatc
     7381 agcagtttat aatgcgtacc gaaatctgcc atcgatacga ctaccccttc tcctgctttc
     7441 ccatcaaata caagacgcgc cggatcttct ctatctccca ttgataaagg agaaacaacg
     7501 attcttggtt tcgtcgcagc aagcgtcgga tctacttcca tcatatggga ttccaagatt
     7561 gcttcttggc cactagctag ctcgtaagta taatcttcca tgaatcctgt atctttccct
     7621 cgagccatga ttttcagcaa ccgatcaatc gctgcagttt tccagtcgcc ttccccagca
     7681 aaaccgtatc cttctgccat caaacgttgt acagctagtc caggtagttg tttcattcca
     7741 tacaggtctt caaaattagt cgtaaaagcg ttatagcctc cttcttctaa gaaacggcga
     7801 ataccgattt cctgctgagc ttgtactttt acatgttcct cccatgtctt ttcttcataa
     7861 tcaccatagt cgaaggtata caattccttg tactcttcaa ataattgatc gacttcttcg
     7921 tctgatacgc gatcgattac ttgtacaaga tctccgatac caaaataatc aacagtccaa
     7981 ccaaattgaa tctgtgcttc cactttgtct ccttctgtga cacccacgtt tctcatgttg
     8041 tcaccaaaac gagccacttt tattccaaaa ctttcattat aggctactgc aacatccatc
     8101 caatcagcaa tctctttttg gatctcaggt cgtttccagt agccaacgac gattttattt
     8161 tgtttattta atcgagcatt gatgaaccca tattcgcggt caccatgagc ggattgattt
     8221 aaattcatga aatccatatc gatcgttttc caaggaatgc tttcgttata ttgcgtagct
     8281 aaatgaagca atggtttttg tagaagcttt gtcccgcgga tccacatttt tgctggtgag
     8341 aaagtatgca tccaagtgat cacacctgct acttcttctc gataattgac ctctttcatg
     8401 atttttgtga tcgtatcagc agtaactgct aattcttgaa ggcggattgg ataaggcaat
     8461 tggccatttt cattcagttc atcgaccatt tcttgtgcat gtttctttac ttctcgcagt
     8521 gcttcttccc catataaatg ctgtgatcct acaacaaacc aaaattcttt ttctccaata
     8581 ttcaacataa ttagccactc cttttatttg ttttgtccgt aatacgcatt ttttccgtgt
     8641 tttcgtaaat aatgtttatc taaaatccgt tgaggcagct cttctgaaaa tgaattgagc
     8701 tgtctggtaa ataaattcat cttgcatact tcatctagta cgactgcgtt cattaccgca
     8761 ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac ccctggaatt
     8821 gctaagggat caattttgcg ttctttgaat gtttctatga taacttttcc tgtttcatat
     8881 tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac cgatccataa
     8941 aaagtatctg catgcgtcgt ccccatcgca ggaacatcca atcctgcttg tgcccagatc
     9001 gtcgcccatg tcgagtgagt atggacgatt cctccgattt gaggaaatga acgatataaa
     9061 acagcatgag taggcgtatc cgaagaggga ttgagtttac cttcaatgat atgattgtcc
     9121 aagtccacaa cgaccatgtc gtctgctgtc atctcttcat aactgactcc gctaggtttg
     9181 ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt atatttgacg
     9241 agtccgtgtt taggcaagtc aagatttgct tggtatactt cttctttcag ttgttccagc
     9301 attttctatc actccttttt cttgcaattg atgaatcgct gttttttcaa tgtcgatccc
     9361 ctcacggtaa cgtctcataa atatctgata gcctgctatt tcttctgctg aaggtgtgat
     9421 ggtgattcct tggtcttgtc caaacacgtc ttctgccaag aagttttcta aagacttccc
     9481 atgtcgatcc atcatatagg ctgctaataa cgcgattccc cacgctccac cttctccggc
     9541 tgtgtccatc actgtaactg gtgcctccat agcagatgct agaatccgct gtccaacttc
     9601 tggtgtttta aaaatccccc catgccccac taaacgatca atttggacat tctcagattt
     9661 caatatatcc attccgattc tcattgcacc aaaagcactg gctaaatgga tccgcatgaa
     9721 attcgctaaa tcaaaacggc tacctggtgt acgtacaaat aatggccttc cttcatttac
     9781 tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt aatcccccgc aatccgtatc
     9841 gccttctagc gctttgttga ataataccgt gaataattta tctctgctta tctccatacc
     9901 caaagaattg gtaaattctt caaaaatact gacccaagca ttgatatccg aagagcagtt
     9961 attcgtatga accatgccta ctagttctcc tgaaggtgta gtgaccatgt cgatttccgg
    10021 atggacattt tctagttctt tttctaatac gatcatagcg aaggcggaag taccagccga
    10081 aatgttccct gtccgtttgg ctacactgtt cgtagcgacc ataccggttc ctgcatcccc
    10141 ttctggcgga caaaccggga ttcctggtct caaatttccc gtagggtcga tcagataagc
    10201 ccctgattcg cttaacttgc cagcttcttc tcctgctact agaactttag gcaaaatgtg
    10261 atccaagtca atcgctacgt gcgctttttc gattaactga ccaaaaatat ttttcatttc
    10321 ttgatggtag gttttcgttg aaggatcgat aggaaacatg cctgatgcat ctcctacgcc
    10381 tagtactttc tcaccagtaa gctgccaatg gacataacct gcaagcgttg tcaaaaaatt
    10441 aatttctttc acatgttctt ctttattcaa aatagattga tacaaatgag cgatactcca
    10501 tctctgcgga atattgtatt gaaatttttc cgttaatatt cgttctgctt cttcagtgat
    10561 tgtatttcgc caagtacgaa atggtactag taactgttcc ttgctatcaa atgctagata
    10621 accgtgcatc attgcactga acccaatcga cccgattgtc gtaagattct cgccatagat
    10681 ttcgaatact tctgctgcca tttttcggta actgacgcgc agtcctttcc aaatatcttc
    10741 taatgaatag gtccagatac cattttctag tttgttttcc cactcataac taccagcggc
    10801 aataggagta aaagatgaat caatcaatac agctttgatc ctagttgatc caaactcaat
    10861 ccctaaagat gtacgaccat ctatgacatc taacatactt tgcgcattta cttccttcat
    10921 aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta gtagcgctta
    10981 caatgattat tgtatacttt tgtacgtaca tgtcaagcgg ttatttacaa gaatacaata
    11041 aaaaacactt gttttgtacg tacataagtt ataaaatgaa attgcttaca aaatctgatc
    11101 aaccgccttc tcgaaattct agaaggagag aatacttatg aaaaaagaaa aaaaagtttc
    11161 ctcatcgttt atttattttt ttggcgcatt tggcggcatt ctatttggat atgatattgg
    11221 tgttatgacc ggcgccttac cttttttaca gcatgattgg ggtttggcag gtaaagcttc
    11281 tttgatagga tggatcacgt cttcggtcat gttaggtgcg atccttggcg gctctttatc
    11341 tggtcttctt tctgataagc ttggacgacg aaaaatgatt cttctttctg cacttatttt
    11401 tatggctggt tctgtccttt ctgcatctgc ccctcacaat ggttcttatt ttctgattgc
    11461 agcaaggatt cttctaggtt tagctgttgg cgctgcatct gccctagttc ctgcttatat
    11521 gtctgaaatg gctcctgcta gactaagggg aagattgtca gggatcaatc aagtgatgat
    11581 tgcttctggc atgctccttt cctatgttgc agattatttg ctaaaagtgt tgcctgaaac
    11641 gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt tatttttcgg
    11701 tgttttagct ctaccagagt caccgcgttt tcttatgcaa tctggccgct tggaagaggc
    11761 aaaaagagta ttgaactata ttcggacacc aaaagaagct gaacaggaat tcgaacaaat
    11821 ccagctgaat gtcaaacaag aaaagacaac cggaacttct tggcatactt tgtttttaga
    11881 aaaatatcgt tccttagtca ttgccggtat tggtgtagca gtattccagc aatttcaagg
    11941 agctaatgct attttttact atattccgct gatcgtagaa aaagcgactg ggcatgcagc
    12001 gagcgatgca ttgatgtggc cgatcatcca aggaatcatt ttagttgctg gttctttgct
    12061 ctttttggtt attgcagaca agttcaatcg gcgaacttta ttgaaaatag gcggtagcgt
    12121 gatgggactt tcattcatcc taccagctgt tttaggaact gtactggatg ctcatacaaa
    12181 ttcgttgttg attctcttgt ttttatgtat ttatgtcgca ttttattctt gcacatgggc
    12241 tccactgaca tgggtaatca taggcgaaat attcccacta gctgtccgcg ggcgagcatc
    12301 tggcctagcc tcttctttta attggattgg ttctttccta gttggcttgc tttttcctgt
    12361 gatgactgct tctatgtctc aagaaatcgt tttcggcatt tttgggatca tctgcttttt
    12421 aggggtttta tttattcaag agatcgtacc agaaactcgt gggaaatctt tagaggaaat
    12481 cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt tctatccatc attcttaaag
    12541 aggttgtgac agaagtggtc agcttcaaga aataagaagg aaccgcttag tttccagtca
    12601 caaggaacaa tttcaaaaaa cagcttctca tgtttgatga atattgtgac ttgtgaccag
    12661 ttggtaggag aacaccgttt agtggagagg tggtgaaaga aacgttcagc cttgagcgat
    12721 gacaaaagct ctcacaaaca ttgcgacgag taaccgtagg agcaataaga ggcggtaaaa
    12781 acccgtctct tatttttcgt tttctttcac tgtttcccgt tcaactagta caggttcata
    12841 gagaatattt ggctgtgcct gtcctttttc aatcgttgtg acgatccacc gtgcggcatc
    12901 ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc ctaaatggct
    12961 taaagaagaa tcgtctgttc ctacgatcga taggtcatct ggaatatgat agccattttc
    13021 tgtcaactta tctagcatta aactagctat ttgatcgtta tagcaaatga tgccatctaa
    13081 agcttgttct tctttcaacc gttgtactgt tttttcagcg attgtttctc gtgtttccgt
    13141 ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta tactgttcac acgcgctgat
    13201 aaaacctttc atcctgtatt ttccttgaag atcgtcgatt tttgtgatga acaacagacg
    13261 ctgatgtcct ttttttatca aatattctgc tgccataaaa ccgactttca catcatctaa
    13321 acaaatataa gaggcattta actcttcata agcagcattg atcatcacca tcggaatgcc
    13381 ttgttctctc aaagcaacat aaagtgctaa gtttgggtta tactgattac ttttcgtagg
    13441 ctcaacgatc aaaccatcta ccccataagc aatcattttc ttcaaacact ctttctccaa
    13501 ttcgtgatcg ttattcgtac tggctaaaag caatgaatac ccagattctc gtaattcatt
    13561 ttcgattcct cggataatcg acggaaaaat ataatcagag agatatgtgg tgatgacacc
    13621 gatcgttttt gttctgttca atggtgtttt ttctttctta tagctgtcat ctacatatgt
    13681 gcccgaccct ttttctttac gcagatagcc ctcatttacc aacacagcga ttgcttgccg
    13741 aatcgtatgt ctgctgacgt catatatttc ttgcagcttg aattctgagg gaatggttgt
    13801 atttacttcg tattcctcac gaaggatctt ttgttttatc tcttctgcaa tatcttgata
    13861 ttttgccatt tgccttgccc ctttttattt gtacgtacat atatcataac gagtctgcaa
    13921 ggtttattca agcaaaaatc aaggtttctt gtgctcataa ggcatacgga acaggttcac
    13981 caaattctgg aaatccttgc tcattccacg taaagcattg cgcgttcgcg tgccgattag
    14041 gattgtctaa cggatcccct ttttggttct tctctggtcg agcatgatag atcaaaatat
    14101 caaagcaccc gtcttctgaa accgtaaacg agttgtgacc aggaccaaat tgcttatttt
    14161 tttcagaaga aacgaatact ggctccgata atttatgcca tgattctttt aataaaagat
    14221 tcttgtgctc atcagcccac agcaggccca tggcatagtt ttcatctgtt gcactaccag
    14281 aatacgtaat gaatattttc ccattgcgag taaggactgc tggaccttca tttactgaaa
    14341 aaccccgttt ttcccattca tactcaggta tagaaaggag gatctgcttc ccctttaacg
    14401 tccaaggatt atccatttcg gatagataaa gattagaatt cccagtaatt tcaggatctt
    14461 gttgggccca cacataatat agctttcctt gagaagagaa agttgttccg tctaaactga
    14521 aagtttccct tttcgttttt atttgtccct tttctatcca atctgtattc attggatcta
    14581 tatgctgatt ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt atagttttat
    14641 gattgcttgc agcaaagtaa atataccatt ttccattgat ataatggact tcaggtgccc
    14701 agatcaattc actcatcggt ccttcttcat gagcctgcca tacaatcagg cttgcagcat
    14761 gttctaattc attcagtgac tttgcccttc ttaattcaat cacttggtag cctggaaccg
    14821 atcctgtaaa ataataataa ccatctgtat gtttatatac ccaaggatct gcccgttgcc
    14881 gaacgatagg attattataa agtgtcttca tcataacaaa tttcctcaca ttttctttat
    14941 tctaattaca ttccaagagt atttttctat ccctacattt aattcgtcat gtttttctat
    15001 tgaaaaatag tttatttcag gttttatttt ttctggttct tcacgtgtat ttttgtccaa
    15061 cagatcgcta ctggttaatg tgtagtgctc tccttgatct agcaaattga aatcatgttc
    15121 aatcgttatc ttttgttgtt catttgtgcg attgatcaag aaaatagtca gctgattgcc
    15181 gttatctacg atcacactgt ccacataagg aacatttttt atcttttcac aatcataaac
    15241 agggcaaatg atttgttgtt tcaatacttg acctttagca tatttcgata agtgttgcat
    15301 cacataataa gttggattcc tccatgcttg tcctttagca tctgtcagta ttaaaggtat
    15361 tgtgtttact aacaatgatt gacaactaat ctttacgcga tctgcgtgtc gtaaaatagc
    15421 cagccctaat gaaccaaata ataatgtatc ttccatagta aagtaacacc aatcgatggg
    15481 actaccgact tcaaaatccc tatgtctttt ttccggttgg gcatgtacat tccattcatc
    15541 aaacgctaga tacattgttt tctcacttct caaatatcct tttacataat cacaagtagc
    15601 aacgatttct tcaatttgac gatcgaatcg ataagctttt gcaaggtatg tcgctgtatc
    15661 atcacgctca tttttaggag gtttagtcaa gtcttcttca tcatacttat caatatagtt
    15721 atgcaaagct aaataatcta cattctcata agcttcttct aatgcgatac gatcccattc
    15781 agggtacgat ggtaaacgtg gcgtagagct tccaactaag attgtttcaa ttgaatcatc
    15841 cactaacttc atgacttttg aggcttcatt tgctaaccta ccatattcat aagcactctt
    15901 agcccccact tgccatggac cgtctagctc attccctaaa caccataatt taacgccaaa
    15961 aggctttttt tgcccatttt ttattcttaa atcactccag aaggttcctt ttggaaaatt
    16021 acaatattct actaaatttt ttgcttcatc aattcctttt gttcctaaat tcaccgtgaa
    16081 tatcggtttc gcttgtacat gactgatcca tttaaaaaat tcgtggagtc caaaagaatt
    16141 tggttctata cctcgccatg ctaaatcaat tttttttggt cgttgttctt tcctacctac
    16201 agtatccacc caatcataac cagatgtaaa gtttcctcca ggatagcgaa tgacacctaa
    16261 ttctaattcc tttacataag agaggacatc ttgtctaaac ccgtcaccat cagctgtttc
    16321 atggcccggt tcataaatcc ctgtatagac gacacttccc atatgctcaa caaatgagcc
    16381 aaacagccga gaactgatct ctcctaccgt aaaatcctct ctgattttta ctttcacttc
    16441 ttcgtccaac agctttccct acctttcatt tcctatagtc aagataatag atacaagttt
    16501 ccatacgcca ccagcaaaga taaaaataaa aggagggaaa agaaatatga gcagtacaaa
    16561 tcctaataac aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata ctttccatgc
    16621 actttgttca aaaatttgta aaaatgtgat atctttgttt tcagaaatca catatatcca
    16681 ggataaaaga aacatggaat aaataagtaa aactacatat ccactaattg aaagtacgat
    16741 tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta acagactaag
    16801 gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa tgatatttcc
    16861 tgatccctca cctttccatt gcgccattac ataaaaaaca gcacatgtag caggcacgat
    16921 agtaataaca ggcaaacaca cgattaccca gagaatacct aataagatgt aatcagaaac
    16981 cacaatcaac tgtccataaa atttagtctc aactattttc atcttcctca ctctttcatt
    17041 ccagacatag ctgttccttg aacaaaatat ttctgtgcaa aaatataaac cactagtaat
    17101 ggcagcattg aaataaccgt tccagccatt acaggtccat aaaaagaaat gttctgccct
    17161 gtaaataaag ctaaccctgc tggtaaagtt ctcatttttg aactacttgt caatatcaat
    17221 ggataaagca agtcattcca actattcatc agcgtgaaaa tccctaatga taaaagagta
    17281 ggtttgctca atggcaacat gattttcaaa aaaatattaa actcacttaa tccatcaatg
    17341 cgtgctgcct cttccaaaga atctggtaaa gtaataaaaa aggaacgcat catgaagata
    17401 ccaaatgcag ttgtcatacg aggaatgatc aaacctgcat aagtatcaag catgccaagc
    17461 aaattgactt caataaatag aggaatcatg aagacttgga atgggatcat cattgtcagc
    17521 aaaacgaaat agaacaaaac tgattttcct ttgaaacgca tacgagcaaa tgcatacccc
    17581 gcaagagaat caaagaatac agaagtgatc actgtccctc cagcaaaaat cactgtattt
    17641 ttgatgtaat ctaataaagg gatcgttgtc caaaccttaa tatagttatc tagggtatag
    17701 ctatctgcaa atattctagg cggaaatgag ataatgtctt tttcgtgttt aaaagaagaa
    17761 ataatcagcc acaaaaaagg aaacgcaata accaacaata taactagcat actaatcgtt
    17821 cgaatgattt ttttaccact aactgctttt cgactattca cagtaactgt aatcgagctt
    17881 tctggtgcca tacaggtttc cctcctattt catttgtttt tctttccgtg acatgtacac
    17941 attagtggag atagctatag ttgcaacaat gataaacaaa accgaagaca gtgctgatgc
    18001 atagcccaat tcataaggtg ctgtaaatcc tcgactgtaa atatattgaa cagctgtttc
    18061 tgtcttaaat tgtgggccac cagcggttgc aacatatact tggtcaaaca cttgcatcga
    18121 accaatcaga ttcgttaaaa tacaaaaacc taaggaggga acgatctgag gtagcgtaat
    18181 gttaaaaaac tgctgtcgat ttgttgcacc atccatttga gcagattcat acaaagaggg
    18241 agagattccc tgaatagcag tcaagagaat cgtcatagta acaccaaaat tcttccaaac
    18301 agtcatgatg gctaccgtcg gcatagcaag ggttggatcg cgaaaaaatt gagggatcgg
    18361 caagccgact tgtgaaaaca agtaaggaat cgcccctaca ttcggatcaa gcaacataga
    18421 aaaaataatc gcaattgacg tcaatgaaca aataactgga atgtaaaatg tcgaacgaca
    18481 aaaacgattg aatctggtat ttttagataa tgctactgct gctaataaac caacgataat
    18541 ttgtgttggc gtttcgagta cggtgaaata taaagaatga atcaacgaat taactgctcg
    18601 ttcatcacta aatatacgtg tgaaattttc aaaacttaca aaatttcttg atgtaaagaa
    18661 aatattcaaa tctgtaaaac taatagcaaa tgttcctaaa agcggtatga cagtaaataa
    18721 taataaaact aaaaaagatg gagttaaaaa caaatacgca gcacgatcag gacgattcca
    18781 atatttggtg aatccgttca tatagtccac ctcatctttc attttgataa aatatacaat
    18841 cttggtttaa acgaatctga ataagatatc cgaacaatcg ctgtgatttt tgcaataaca
    18901 cctatctgca aatactaggt cgatcattca gatatctgta tcttgccaac agtctatcct
    18961 gtataaagtt ctgtactatt tttgcagtaa ttgattgatg gcatcatctg catcgttcat
    19021 gagtgtctca ggatcttctc ctgctaataa tttttcaatc agtggattga tgatatcgtt
    19081 attgatcgca gagatttggg taaaacctgg taaatatggc tctgcatagt ccatttgttt
    19141 cgataattca gatacaatag gatcattttt tacttcttca tcttttgcta ctgaatccaa
    19201 gtaggcagga aaaccatttt cgatactcca ttttttacca atttcagttg tattccaata
    19261 tttcaaaaac tcatagacag cttctttttt attattatct gtacttgccg gaatggcata
    19321 tcctacgcca tctaaaatag ctgttttttt gtctgaatca ctcccttgtg gtaaggtagt
    19381 cacattataa tcaatctcgt ttgctttcaa tccgttattt aaccatggcc cattgatttc
    19441 aatggctagt tgaccagcat tcattaaatt gtccatttcg gcgccagaaa tcgattctgg
    19501 accagtcttg tctacatgga tcattttttg gatcgctttt aacgttttaa ggttctcagg
    19561 agacgcaaat tcagacttag tataatcatc gttcactaat ttaccgccat ttgctaacat
    19621 ccagttatat aacggtgcag ttccatcctt attgaagaca aaacctgata catttttact
    19681 actatcagtt aattttggtg ccatttctgt caattcttcc caagtagtag gtggttgttc
    19741 agtatctaat ccagcttgtt taaacaaatc attattccaa tacatataca tgccttgtac
    19801 ctgcatgggg acaaaatact gtttaccttc tacttcacct aattcaatag ccgtttcttt
    19861 aaaatcagat ttatctactc catcatattc ccagaaatcg tctaatggtt gtaccgctcc
    19921 gttttctaca tactgtgcaa aatctggata atttaatgca ataaagtcag gtgctttttt
    19981 agaagaaatc gctgtaggaa gtttttcatt caaattggcc caagtcatga tatccatttc
    20041 aattttgatg tttttatcat ttgttttatt aaattcatta acgatttctt gcaatatttc
    20101 accatcagat gcagtaaacc cattccaaaa agtcaaagtc acttctttat cagaatcaga
    20161 ttctgctgaa cttccgccac cgcaagctcc taaaatacct gttgcagcta ccaacaaaga
    20221 gccaattaac cattttttac ctttcatttt ttgttcctcc ttcatctgat aaacgacatc
    20281 tgaccacatt ccaagatagc ggtttcaata aacaattgat tttcccatct tcagctttta
    20341 catttgttga atgacttggt tttacttgtt cacttttagg aagattagtt gcttttgtat
    20401 caaatccact catttcagaa aaatcaatca ctccctctaa tgataattca gacaagtgaa
    20461 cagttagatt catttcttct tctccacggt taactgcaaa tatgacaagt tctttgtcat
    20521 ttattacggc tatactttct aaatacggca cttcttcaaa atcttccgtc gcatagtttg
    20581 ctgaacgaat ttgtgggctt aacacctctc cttgtccata cacagcagct tgcataaatg
    20641 gataatagat cgtctgtctc caagcttctc cgtttgtttc tgtcctgatt ggtgcaataa
    20701 cgtttaccaa ttgagcaaga caagcaatct ttactctatc tgcatgtttc aataaagtga
    20761 ttaatagaca gcctaatagt aatgcatctt caaaattata gttatcttct agtaaaggag
    20821 gagcaatctg ccaaggcgct gcttgctcat cttgttccgc tgaatgatac caaatattcc
    20881 attcatcaaa ggacaggttg atctgcttct tactatgttt cttcgccttg actgtatcgc
    20941 acatcgctgt cacttcagaa atgaaccgat ccatgtctaa ggaacgtgca agataatgag
    21001 aaagatcatt tttttggttg ccataatatt gatgcagaga caaataatct atttgatcgt
    21061 acgcttgatc aagaactgtt aactcccaat caccaaatgt aggcatttgc cgattcgaac
    21121 ttccgcataa aaccacctca attgaatcat ctacgagttt catcgcctta gccgtttcct
    21181 ctgctaaccg tccatattct tccgctgttt tatgtccaat ctgccaagga ccgtccattt
    21241 cattccctaa acaccatgtc ttaattccaa aagggctctc ttgaccattc gaacgcctta
    21301 aatcactcca ataagttcct ttaggaaaat tacaatattc gactaaatta cgagctgcat
    21361 ctactccacg agtacccaga ttgacagcca tattgatttc ggcccccaca tgctgacacc
    21421 agttataaaa ttcatgaatc ccaacttcat tcgtttcaat tgtccgccaa gctaaatcca
    21481 accttctagg tctatttgct atgggcccaa tactgtcttc ccaacgaaat cctgacacaa
    21541 aatttccacc aggatatcta atcatgggaa tgttcaattc ttttaccaag tgaatgacgt
    21601 cttggcgaaa tccactttta ttagctgatg gatgatctgg ctgataaatc ccttcatata
    21661 ctgctcgtcc catatgttca ataaatgaac tgtacaaccg cggatcaact ttgcctacac
    21721 gcttgacact actaatcaac attgtggctt ccataactga cctcctttaa tttatacatg
    21781 aataatacac ctcgttgaaa gcgttgtcaa taatataaat gttaaactta cttatttttt
    21841 atcgatttta attaaccaaa atattaataa tgattaaaac aaataattta atataacttt
    21901 tattaacaaa tatgttaata tataagaaaa accttttaag aggtgatata tatgcaacta
    21961 caaataaacg aagaatcctt accggtatat gaagcattag ctagtaaaac aaggatcaag
    22021 atcatccagc tactttccaa aaaaaagatg aatgtcaaag atttggcaaa agaacttgga
    22081 gtgagcagtg ctatcacgac gatgcacgtg aaaaagctag aggaagcaaa catcatcaaa
    22141 acagaaaaag taggacaaca aaaaatttcg agccttcgtg tcgataaaat cgatattagt
    22201 tttccagaaa aaatattcaa tgcttttgat acgaaagaaa catcgatacc tattggtcac
    22261 tatacaaatt atgcaattga accaacatgc ggtttagcta cgatccatga ttttatcggg
    22321 aaagtggatg aaccacgcta ttttatggat cctcgaagga tggatgcccg cattctctgg
    22381 tttaccagcg gatttgtcga gtatcaggca cctaattttt taaatacaga ggatacattg
    22441 gagatgttgg aagtttctgt ggaaatttct tctgaatttc ctttttcaaa tgacaattgg
    22501 ccttcagata tcactttcag cctaaatggt gtggagctag gaacatggac gagccctgga
    22561 gattttgctg atatccgcgg taaatatacg cctgattggt atccagataa tcttaatcaa
    22621 tacggcttgc tgaagacgat ccgaattacc aaacacttga cgaatatgaa tggagaaccc
    22681 ttgtcaaaca tcacgatcaa cgacatccct aaagagcaag acacttggca tttgcgaatc
    22741 gaagtgaaag acgatgccaa acatgtgggg ggctgtactt tatttggtaa aggattcggt
    22801 aattacgatc aggatatcaa attaaaagtt tactacagct gatatttttc ttttctattt
    22861 tataaaacaa aaacagccaa tccctatgaa gggacggctg tttttgcttt attgttattt
    22921 ccagaaatca tccaaaacgg aaattggcaa atgtcgttta tgttgtgtct tgttccacca
    22981 tgcttcgatt ttttcttgtg cttgagcggg aacttctttt ccttctagat aatcatcaat
    23041 ttcattatat gtgacaccta gtgcgacttc atctgcaatc attggtttcc catcttcaag
    23101 atctgcagtc ggaatttttg tgtataattt ttccggtgca ttcaatgctt gaagcaattg
    23161 tttcccttgt cgtttgtcca aacggaataa tggcaaaatg tcggctccac catcaccaaa
    23221 tttcgtgaag aatccagtaa tgttttctgc agcatgatct gtccctaata cggctcctgc
    23281 tctttctcca gctacggcat attgcgtaat catacgttga cgtgctttga tatttccttt
    23341 gttaaaatcg ctgattccca cacctgcttc gcttaatact tgtacttgtg catcaacagc
    23401 tggtttgata tttactcgca gactaacatc gggctggata aagtcaagag cagcttttgc
    23461 atcttcttca tctgcttgtt caccataagg aagacgaaca gcgataaatt gatagttttc
    23521 atcttttgtt tcttcacgca tttcttccat cgttagttgc gccaaacggc ctgctaaagt
    23581 agaatcttgg ccgccgctga tacctagtac aaaagtttta agaaacggat attttttcaa
    23641 atatgctttc ataaagtcaa tacttttacg tatttcttct tttggatcaa ttgttggttt
    23701 gacacctaat tcattgataa tttctgtctg caggctcatt tattctcctc ctatgcttct
    23761 gtatgaagtt ctgctacgct ttttttcatt cgttccatga tagccatttt atggttccag
    23821 cattcagtag acagatcgac tgggtatttt tgcggattca agtctcgttt atactcttcc
    23881 catagtgaat ctagattttc ttttgtatat tctttgattt cgtttagtgc aggtaattcg
    23941 taaactcttt ttccttcgat aaagatatct tgtaaaactg ggcgagctgt aaagtctcga
    24001 acagttttat tgataaacgt atgcactgga tggaacatga agatagcttc ttcttctcga
    24061 gggtcttcat cccataatgt cacgtagtct ccttcagatt tcccatcaaa atttcgagtg
    24121 atgcgccaca cttgtttttt ccctggagta gttacttttt ctgcattgct tgataacttg
    24181 atcgtatcca tcatttttcc ttcatcatct tcgatcgata ctagcttgaa tactgcacca
    24241 agggctggct gatcataggc tgtgatcaat tttgtcccaa caccccatac atcaatttta
    24301 gctttttgca tctttaaatt caaaatcgtt gcttcatcca agtcattcga agcatagatt
    24361 tttgcttccg taaatccggc tgcatctaat tgttcccgaa cacgcttaga tatataggcc
    24421 atatctccac tgtcgatgcg tacgccttgg aagttgattt tgtcacctaa ttctttcgcc
    24481 acgcgaatcg cacttggcac acctgatttc aacgtatcat aagtgtcgac taagaacacg
    24541 cagtctttat gggttttagc gtaggccata aatgcttcat aatcatttcc ataagattgg
    24601 actaacgaat gagcatgggt cccgctagct ggtatcccaa atatcttccc agcacgtaca
    24661 ttgcttgttg catctgctcc gccgatatat gccgctctag ttccccagac tgctgcatcc
    24721 agttcttgtg cccgtctagt tccaaattcc aatagtggat cttctccgat gacagatttg
    24781 atacgagctg cttttgtcgc gatcaacgtt tggaaattga ccatattcaa cagtgctgtt
    24841 tctactaatt ggcagtgcgc taaaggccct tccacttgga tcaatggttc atttgcaaaa
    24901 actagttctc cttcacgagc agaacgaact gttgctttaa attcaaaatt ttgtaaatac
    24961 tccaaaaaac cttcaggata gacttctaat ccacgcaagt aagcaatatc ggaatcattg
    25021 aacgtcagat tctctaaata attgactaac cgttccaatc ctgcaaaaac cgcgtatccg
    25081 tggttaaaag gcatttctcg aaagtaacat tcaaaaacag catgtaaatc agctcgacct
    25141 aattcccagt atgtctgcat catattgatt tgatatagat ccgtatgcag cgtcaaactg
    25201 tcgtctgggt aagtattatt catcttgtat tactcctctt ggcaagtttt catttcctac
    25261 ccattataac aaaaaaaatt aataggaaaa gaaatcttcc attttttctg aaaaagcaca
    25321 tgttttacgg atatttcttg ctaaaaatga actccatctg ctaaaactat cctttcttat
    25381 tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg agacacgaag
    25441 aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct actacaatcg
    25501 tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca acataatcaa
    25561 tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat ccccaaagaa
    25621 acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga caagatatac
    25681 cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat acaaagtaag
    25741 aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt ctcgccttgt
    25801 tttttattgt atacattaga tatggagcag gttaccataa agatgattat tagtcgttat
    25861 taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt tggacgtttg
    25921 aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt gacatcttct
    25981 cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt gatttttttc
    26041 ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag cagttgtgtt
    26101 cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa gaattccact
    26161 actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc gattagcttc
    26221 atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat gttaccatct
    26281 ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga tttacctttt
    26341 atcacttaat agtataacat aatttcatga gaaaaaaata caattttttc ttgaccatct
    26401 cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag tacacaataa
    26461 agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat gatgagattg
    26521 gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc aaaaaagaag
    26581 tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg gatgatatcc
    26641 aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag catcagcaaa
    26701 tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa cagacacttg
    26761 atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg tttaaacaac
    26821 aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa tacggaaaaa
    26881 aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg tatgaagaaa
    26941 tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa gaaccagaag
    27001 atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttatct tacagctggc
    27061 ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc gatgaacggt
    27121 tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta aatgaaatta
    27181 tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta aaatgaccta
    27241 ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg tctgctccat
    27301 tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa acgtataagg
    27361 tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt ctccgcaagc
    27421 ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca aattatcgcg
    27481 tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg ctgcaaccat
    27541 atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc ctgcttgttt
    27601 tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag atggaaaaag
    27661 ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac ttcgccccaa
    27721 cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt ctgtaattga
    27781 ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt cacaaaaaac
    27841 tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac gagacgtaat
    27901 gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct ctttttcttt
    27961 ctgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg cttcaatgat
    28021 cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca ctccaatgct
    28081 tttcgttttt ccataactta actgtctggc atttgcatta gggatataat cgacttcttt
    28141 catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc cattgattac
    28201 acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa tgtttgccac
    28261 tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac agctggaccg
    28321 cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg cccgccccaa
    28381 tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac tatattccta
    28441 ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac aaacgcataa
    28501 aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc aagccctcct
    28561 tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt gttttgccaa
    28621 agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat tgaatacatt
    28681 ataatgatac tgtaaatata tctttattat tagtgaatgg atatttactt ttaatagata
    28741 aaatattata actattaaaa ataactattt tcattataat tacaca
//
LOCUS       DAZKOB010000022        28603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000022 DAZKOB010000000
VERSION     DAZKOB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.022"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1877
                     /locus_tag="ACG8M6_001015"
     CDS             237..1877
                     /locus_tag="ACG8M6_001015"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0456702.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="ACG8M6_001016"
     CDS             2031..3353
                     /locus_tag="ACG8M6_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0456703.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="ACG8M6_001017"
     CDS             complement(3415..3846)
                     /locus_tag="ACG8M6_001017"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0456704.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="ACG8M6_001018"
     CDS             complement(3910..4746)
                     /locus_tag="ACG8M6_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0456705.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="ACG8M6_001019"
     CDS             complement(4936..5238)
                     /locus_tag="ACG8M6_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0456706.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8M6_001020"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="ACG8M6_001020"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0456707.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="ACG8M6_001021"
     CDS             complement(5692..5958)
                     /locus_tag="ACG8M6_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0456708.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="ACG8M6_001022"
     CDS             complement(6212..6937)
                     /locus_tag="ACG8M6_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0456709.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="ACG8M6_001023"
     tRNA            complement(7303..7373)
                     /locus_tag="ACG8M6_001023"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="ACG8M6_001024"
     CDS             7490..8242
                     /locus_tag="ACG8M6_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0456710.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="ACG8M6_001025"
     CDS             8258..8737
                     /locus_tag="ACG8M6_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0456711.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="ACG8M6_001026"
     CDS             complement(8988..10568)
                     /locus_tag="ACG8M6_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0456712.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="ACG8M6_001027"
     CDS             complement(10561..11304)
                     /locus_tag="ACG8M6_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456713.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="ACG8M6_001028"
     CDS             complement(11480..12256)
                     /locus_tag="ACG8M6_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0456714.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="ACG8M6_001029"
     CDS             complement(12504..13340)
                     /locus_tag="ACG8M6_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0456715.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="ACG8M6_001030"
     CDS             complement(13351..14280)
                     /locus_tag="ACG8M6_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0456716.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="ACG8M6_001031"
     CDS             14472..14825
                     /locus_tag="ACG8M6_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0456717.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="ACG8M6_001032"
     CDS             complement(14899..15555)
                     /locus_tag="ACG8M6_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0456718.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="ACG8M6_001033"
     CDS             15781..17715
                     /locus_tag="ACG8M6_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0456719.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="ACG8M6_001034"
     CDS             complement(17801..19024)
                     /locus_tag="ACG8M6_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0456720.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20889)
                     /locus_tag="ACG8M6_001035"
     CDS             complement(19018..20889)
                     /locus_tag="ACG8M6_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0456721.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="ACG8M6_001036"
     CDS             complement(20910..22028)
                     /locus_tag="ACG8M6_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0456722.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="ACG8M6_001037"
     CDS             complement(22111..22944)
                     /locus_tag="ACG8M6_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456723.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="ACG8M6_001038"
     CDS             complement(22937..23854)
                     /locus_tag="ACG8M6_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456724.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="ACG8M6_001039"
     CDS             complement(24081..25205)
                     /locus_tag="ACG8M6_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0456725.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="ACG8M6_001040"
     CDS             25388..26296
                     /locus_tag="ACG8M6_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0456726.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="ACG8M6_001041"
     CDS             26437..28050
                     /locus_tag="ACG8M6_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0456727.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5622 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta aca
//
LOCUS       DAZKOB010000023        27683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000023 DAZKOB010000000
VERSION     DAZKOB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.023"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(130..657)
                     /locus_tag="ACG8M6_001042"
     CDS             complement(130..657)
                     /locus_tag="ACG8M6_001042"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0456728.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(724..2226)
                     /gene="eat(A)"
                     /locus_tag="ACG8M6_001043"
     CDS             complement(724..2226)
                     /gene="eat(A)"
                     /locus_tag="ACG8M6_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0456729.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2484..3605)
                     /locus_tag="ACG8M6_001044"
     CDS             complement(2484..3605)
                     /locus_tag="ACG8M6_001044"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0456730.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3731..4672
                     /locus_tag="ACG8M6_001045"
     CDS             3731..4672
                     /locus_tag="ACG8M6_001045"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0456731.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4822..5718)
                     /locus_tag="ACG8M6_001046"
     CDS             complement(4822..5718)
                     /locus_tag="ACG8M6_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0456732.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5987..7327
                     /locus_tag="ACG8M6_001047"
     CDS             5987..7327
                     /locus_tag="ACG8M6_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0456733.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7545..7988
                     /locus_tag="ACG8M6_001048"
     CDS             7545..7988
                     /locus_tag="ACG8M6_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0456734.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8492..9910)
                     /gene="nhaC"
                     /locus_tag="ACG8M6_001049"
     CDS             complement(8492..9910)
                     /gene="nhaC"
                     /locus_tag="ACG8M6_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0456735.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10313..11536)
                     /locus_tag="ACG8M6_001050"
     CDS             complement(10313..11536)
                     /locus_tag="ACG8M6_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0456736.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11567..12406)
                     /locus_tag="ACG8M6_001051"
     CDS             complement(11567..12406)
                     /locus_tag="ACG8M6_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0456737.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12423..13103)
                     /locus_tag="ACG8M6_001052"
     CDS             complement(12423..13103)
                     /locus_tag="ACG8M6_001052"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0456738.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13105..14178)
                     /locus_tag="ACG8M6_001053"
     CDS             complement(13105..14178)
                     /locus_tag="ACG8M6_001053"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456739.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14234..14472)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14515..14748)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14825..15496)
                     /locus_tag="ACG8M6_001054"
     CDS             complement(14825..15496)
                     /locus_tag="ACG8M6_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0456740.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15583..18231)
                     /gene="mgtA"
                     /locus_tag="ACG8M6_001055"
     CDS             complement(15583..18231)
                     /gene="mgtA"
                     /locus_tag="ACG8M6_001055"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0456741.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(18573..18747)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            18920..19357
                     /gene="ndk"
                     /locus_tag="ACG8M6_001056"
     CDS             18920..19357
                     /gene="ndk"
                     /locus_tag="ACG8M6_001056"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0456742.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19379..20245)
                     /locus_tag="ACG8M6_001057"
     CDS             complement(19379..20245)
                     /locus_tag="ACG8M6_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0456743.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20405..22498)
                     /locus_tag="ACG8M6_001058"
     CDS             complement(20405..22498)
                     /locus_tag="ACG8M6_001058"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0456744.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23247..23870)
                     /locus_tag="ACG8M6_001059"
     CDS             complement(23247..23870)
                     /locus_tag="ACG8M6_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456745.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(24029..25426)
                     /locus_tag="ACG8M6_001060"
     CDS             complement(24029..25426)
                     /locus_tag="ACG8M6_001060"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0456746.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25458..26429)
                     /locus_tag="ACG8M6_001061"
     CDS             complement(25458..26429)
                     /locus_tag="ACG8M6_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0456747.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26803..27228
                     /locus_tag="ACG8M6_001062"
     CDS             26803..27228
                     /locus_tag="ACG8M6_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456748.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         7975 a         5729 c         4814 g         9165 t
ORIGIN      
        1 taaaataatt aaatttcatt tctaaaggtt gagtttatat tatcaaaaga attgtcataa
       61 cctaaaaaag gcactgctaa gcaaaagcag tgccttgaaa tattttattt aaataacaca
      121 aaaacataat tactcataag aaaccaattc tgtattatct accatgaatg taaagaaccc
      181 ctcattaggc atcggatgaa aataagcatt gatcactctt atcaaggcat tatggcaaac
      241 taacagatag acattttctt cgtcttcgat gcattctttc aataaaggaa cgatccgtgc
      301 atacacatct aagactgact ccccatttgg gaatcgtaca gcgaacgcta aacgtgcttt
      361 ttgaaagttc tcatctttgc ttggcagtcc atcataatcc ccaaaatcca tttctaccaa
      421 tcgttcatca acggtcagcg gcaagctgag acgatcagca actgcttgtg ctgtatctct
      481 tgctcgttga agaggggaat ggatgatttt cgtgattgga acaggcaaat ctactacctt
      541 ttctgctagt ttctgtgcct gcatctgtcc tttttcagtc agcggcacat ctgcccgtcc
      601 gcaaactaat ccttgtgcgt tccaagttgt ttctccgtgt ctcgttacat aaagcatgtg
      661 attctctcct tcgttggcaa agttcccctt aaccatacct tgttgttctt gagattacaa
      721 gggttagatt ctctcaagag aaataatctc agtagatact ttatccagga aggtttgatc
      781 atgttccact agtaacatgg caggtttcac gttcaagatc agttgttcta attgttcttg
      841 attgaagaca tccaaataat tcaatggttc atcccatgta tatagttcag ctggctgtga
      901 taaagattta gccaattcca cttttttccg ttggcccata ctcatctgct cgatcttgtt
      961 atgaaaaaca tctctttcca tcccaagctt tcggaggttg ttcaaaaatg cttggtagtc
     1021 tacttggttt ttctctgcaa attccgccaa cgttcctcga ttgtcttcat aattttggct
     1081 tgcataacta atgctcagat gttttggctg gtatttttct cctataacct tgccgttgaa
     1141 tgcccccaga agataatgga taatggatga ctttcctgca ccgttaggac ctgaaatcgc
     1201 cacccgctga tgaggctcga ttgtaaaatg aattggctcg aataacagat tttcataccc
     1261 taattgaaga tcttctacgc ttaaaagtcg cttatggtga gacgcttggc tattcatcgt
     1321 caaagaatcg atatattcga tatcttttaa tagtttttct ttctcgctga tctgggtctc
     1381 catccgattc acgattgctt tggatcgttt catcgtccgt gcagcatcag cacccactgc
     1441 tcctttattc actcgtctag attcagtatc gatgaatccg acttgtttct ttgttttatc
     1501 tccttctcgg gaacgagacc attcggcttt ttcagctgct gttttcttta gcctactgac
     1561 ttcttttttc aatttttcat tttgagccat ttcaaattca tcacgaagtt ttttctgttc
     1621 ttcatagata gagaaattcc cttgataaag ttccagttga cttttttcga ttgctaaaac
     1681 atggtccact acttcatcga taaatccccg gtcatggctg accacgatga agccttgttt
     1741 tttctttttc aaataagccg ctacttgttt tctaccagag atatccaaat gattcgttgg
     1801 ttcatcgatt aacgggaaat gagtgtcatc cacaaataaa agtgccagta ggacttttgt
     1861 cttctctccc cccgatagtg tgctgaattc tctccataag atttcaggat ctgtttgcat
     1921 caattggagc tctctttcga tttcccatat ctcaaaatcc gtaatatcat ttaacacgta
     1981 ataggttaaa cgttctttat cttttgtctg ttgcgggaaa taggcaaatt cttgctgatg
     2041 gattacttgc ccttgataag gtagtttgtt ttgtagaata ttcagtaaag ttgtctttcc
     2101 tcgaccgttt cgtccgataa gtcctagttt ccattgtgtg tcaaaattta gatttgcttg
     2161 ttcaaataat aatgtgcctt ggctgtcgta gccgaatgtc agatttttta tttcgatttt
     2221 agacattttc catcacctcg taaaaaaaga ccagtcctat tttcagaact ggctaaagag
     2281 gatgcaaggg ttggatacac acaaaaagac ccatacatcg aaaaaaccaa gccaatcccg
     2341 aaaatctgca cactgcttcc ttgtctccac aaggtacagt ctgaatgcag ctttcatcaa
     2401 tcgacttaat ttttcaatgc acggatccct cactttgtcc tatatttgaa attacgatag
     2461 cataaagaaa atgattttgt caactacttc gggaggatgg tgaaaaatct gctaagtgtc
     2521 tgtcctaaga tttttgtttt ttctaatgtt tgcctgtgtt ccattgcttc ttctaaacct
     2581 attggaccca aatgaatact taagacaatt gggagaaaat cagaaagtcc ttctagctct
     2641 tggtctctac ttccgcaaat agcgattatt ggttttcgat atttttttgc aagcactgct
     2701 acaccgtatg gaacttttcc gtggatcgtt tgctgatcga tccgcccttc tcctgtaatg
     2761 atgacatctg cttgttcgat ctttttttcc aatccagtga tttggctgac taaatcgaaa
     2821 ccagaaacca tcttcgctcc caacaaaaaa agggctccac caataccgcc tgctgctcca
     2881 cttcctggaa tatcatctaa atccacctcc cagaaatctt ttatttgctg agcaatcttt
     2941 cctgcttgtt tatctaagaa agctagcgtc gtatctattc ctcctttttg tcttccaaat
     3001 tgccaagcag ctccttgttg acctgtatat ggattggtaa catctgcagc gataatcaat
     3061 tttactttag aaacaagaac gtttcttcga tcaaaatgaa gttttttagt agataataaa
     3121 ggatttcctt ctgtgtaccc ttctaaagaa gcacccaagg attggagtaa tcctagacca
     3181 ccatcgctac acccgcttcc tcctaaagtg acaataattt gttctacttc ttgaagcagt
     3241 gcatctttaa taagttctcc taaggcataa ctggaagcaa gtcggatagt ttgatccgtt
     3301 ggtttatcaa gtagatctaa ccccaatgct tgtgcagatt cgatgactgc ggtgtttttt
     3361 ccttgccaag ttgtcaataa atagctgatt tttcgctgac ggaataagag atccatccca
     3421 tcaatggtac gccatgttcc cctgagcgcg taatagattg cttctgcagt attttctccg
     3481 ccatctgcaa tcggcacatt gatttttctt cctttacgat cttcccagcc atttagaact
     3541 gcctgattta gttcaaatga agaagcactt cctttaaatg aatcaattgc cgccaaaata
     3601 tccatctgta tccctcactt tcttcttaag taagacactt tttttcattt ttttcaaaca
     3661 aaacaatgct attttcttcc atttttctat atactaaaag ggaactcatt gaataaagga
     3721 gagaaaaaac atgaaaatcg caatagctgg ggccggagca atgggcagcc gaatcggatt
     3781 gatgctacat caaagcggaa atgaggtcct cttgatcgat cgttggcctg ctcacattga
     3841 agccattcgt acaaatgggt tgattgctga ctttaacggt aaagaagtgg tcgcaaaact
     3901 gccaatctat tcaccagaag aaatcatcga aagcaacgaa cacgtggact tgatcgtagc
     3961 attaacaaaa gccaatcaat tagatgatat gttttgttcg atccaatcca ttattactga
     4021 caatacttat gttttgtgtt tactaaatgg attaggtcat gaagatgtac tggaaaaata
     4081 tgtgccaaag aaaaatatct tatttgggat cacgatgtgg acagctggtc tagctggtcc
     4141 cggaaaagtc acattgttag gtgatggaga aatcgaacta gagaacttgg aaccagaagg
     4201 cgaagcattt acaaaaaaag ttgtagaagt ctttcaagaa gcaaatttaa atccgatcta
     4261 tagccataac gttcgttatt ccatttggcg aaaagcttgt gtaaatggaa cattgaatgg
     4321 tttatgcacg attttggatt gtaatattgc tgaactaggc gcgcaaaaag cggcagagag
     4381 catggttcga acaatcgtat ctgaatttgc ttctatcgca gcaaaagaag gaatcatact
     4441 agatcaagaa gaagtgtacc aacatattgc ttctacttat gatcctgaca acatcgggtt
     4501 gcattaccca tctatgtacc aagacctgat caaaaaccat cgtttgactg aaatcgacta
     4561 catcaatggt gccatttggc gaaaaggaca aaaatatgat attgctacac catattgtgc
     4621 ctttttgacc caattagtcc atgcaaaaga aggaatcctt ggcgcagaat aaaaggctct
     4681 ctgatgagaa tcagctgaat cagcagctgg ttctcttttt ttaagcccaa taaaagatca
     4741 aaaagaaaac acaactctca tgatgccata agaattgtgc ttgtttgttt cagtacataa
     4801 tctcttcaaa atgtaggttt tttatttgtt ttcaacagag gagaggtcat attcgtattg
     4861 ctcataaata gtggaatcgt tagtgataaa tcgttttgcc caattttctg catcataaaa
     4921 ttccaatagc tctgatagga ttggcttaaa tggtgtatgc agaaattccg gtaattcatc
     4981 taaatcacgt tctcgaagca aaaaaatatt ttcaggacga taaaaatgat aatgagcaat
     5041 cgcttgatta tccgtcacta gtttcttttg aaaatacatg gattcaaatg tccgcaaagt
     5101 cactccttgc tgaccctccc gcaacagttc cagaatcccg cgcgattccc ctgttttttt
     5161 caggtactct tcataagtaa gatatccagg ttgttcattc cctcgatgtg gaagaatgaa
     5221 atagttggtc gtaacatctc tttttataaa ttcctgctta tagctttctg ctcgttcttt
     5281 tctcccatta ttcgttgcac caaaaaatag atccgtcttt ggttcgctag aagaaaaatt
     5341 catatgtttc gaataaaatg aactattatg tttcagccca agttcttttg cttccaaaaa
     5401 atcaaaatga taaatctcat cgattgccgg ttcatcttgt gcctgcttca gcatcgcctc
     5461 gtgttctgct ttgaagtgat tccaaaaaaa taacacaatt tttgcattgg taaatgggcg
     5521 cacgcgtcga gccatctcca tatcaaatcc ataatctaaa aaaacgatca gctgataatt
     5581 tgccaactgt tgtctagctg atttaatgaa ttccgactgt agtaaatctt ttccggacca
     5641 aacatctaaa gagcggatgg aaggcaaaaa ataaagtgat ttattttttg caactagcag
     5701 tacgtcattt ttgttcatct tttccgcctc actttctctg tcactttttc ctatgatagc
     5761 ctgtttaaat agtaaaaagc cagtaaaacg tttcctatat ttgtatgaaa atttaacatt
     5821 tgtatgaaaa tatgatgaaa taaatcattc ttctattaaa attcgcctaa aagttttctg
     5881 aatagttgta attgtctgaa aatttgttat cattagtcat tgttaaatat tgctatttat
     5941 tcttaatata aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa
     6001 attgaaaaaa caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg
     6061 tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat
     6121 tgcttttttg attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt
     6181 tttaagcggt ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc
     6241 tcattctgct gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc
     6301 tgaagctgtt gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt
     6361 atttgttttt atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt
     6421 cgcagaaaca gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat
     6481 ttttgctact tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca
     6541 gcaattgact gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt
     6601 gcttgtagtt atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc
     6661 agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc
     6721 cttttacatc attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg
     6781 cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga
     6841 tatcgtcaat tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc
     6901 atcttcaaga attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc
     6961 tgttttaaac aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta
     7021 tcttggtgta gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc
     7081 cctttcgtat acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc
     7141 actaagaaaa ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg
     7201 attgattttc cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct
     7261 gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa
     7321 tgaatgaaat cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga
     7381 aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc
     7441 tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca
     7501 tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga
     7561 agtttttgtt gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg
     7621 tctatgcgct cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg
     7681 gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga
     7741 taaatgttat gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac
     7801 gaagccaaac tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta
     7861 tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg
     7921 aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa
     7981 tggaatgacc attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca
     8041 agaaataaga agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc
     8101 tgcatcaagt aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag
     8161 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg
     8221 ttctgacctg agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt
     8281 tgagctaata ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc
     8341 gcttagtttc gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat
     8401 ttggcttaat accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac
     8461 aaaagtcttg tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta
     8521 ctgcttcttg cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca
     8581 tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa
     8641 taccaaatgt acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta
     8701 ctgttccacc atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat
     8761 aagtttcttt aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa
     8821 tatttactcc gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct
     8881 ttcttgatac tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta
     8941 atgacaaagt tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc
     9001 taagcagtga atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc
     9061 cagccaactt cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag
     9121 cgatattcaa caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta
     9181 aaaataaagg aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca
     9241 cttcacgaat agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa
     9301 agaaagaaac aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca
     9361 aatcggcatc aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat
     9421 ctccaaaaac ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg
     9481 ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta
     9541 cagcgctacc tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga
     9601 tcaatttgaa cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa
     9661 tcaatgaacc gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga
     9721 tgattccttc gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca
     9781 ttccgatcgc tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg
     9841 caatgcctaa aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt
     9901 tttttcccat gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata
     9961 tcggattact ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt
    10021 tttaaactaa ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc
    10081 actttcaaac ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat
    10141 cggttcacag cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt
    10201 tgacttgtac gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt
    10261 tcatttttct gaaaattatt tttataactt tcacacacaa gattattttt tactagaaag
    10321 tagcgccctc ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta
    10381 atagctttgg ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg
    10441 caatagaagc aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg
    10501 caatatatgt ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat
    10561 ttcctacatc agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa
    10621 cacctgcttc ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat
    10681 catcaactgc attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac
    10741 ctcgtacgat attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac
    10801 gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg
    10861 taatgatccc atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat
    10921 taaacacttg gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat
    10981 gggaagcgac accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt
    11041 tcccataacg aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt
    11101 ctcgaacaaa gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat
    11161 atacacggat ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata
    11221 cggagtaagt accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga
    11281 attctgccaa aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg
    11341 ctgttcggtg tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa
    11401 tcaacgctgc tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta
    11461 gagcaagtgc ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg
    11521 agattgttgt tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga
    11581 atgtttccca tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt
    11641 cttctgtttg ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc
    11701 ttgcaacgat gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg
    11761 catcagaagc aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt
    11821 ctacatctga caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt
    11881 ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga
    11941 tcaatccagc agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga
    12001 ttgctacttt tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc
    12061 ccagcggtgc attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt
    12121 aattgtctaa aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag
    12181 ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt
    12241 tcaaacggtc tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta
    12301 cgatagtttc ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa
    12361 taattgctac aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac
    12421 tttcaatgtg taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag
    12481 attagaatca ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc
    12541 gatatagcaa gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg
    12601 atcacgttga tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa
    12661 tacacacgta aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc
    12721 ccaggatcaa cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca
    12781 aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta
    12841 gtcaatgcta aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc
    12901 ttatcaagta tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact
    12961 aacgtcacac ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata
    13021 tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa
    13081 tatgttttaa agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc
    13141 taaatcgttt ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc
    13201 ttttttccgc tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc
    13261 ttgtaataac tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag
    13321 atgagcgatt agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt
    13381 attggcgatc tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag
    13441 atgagtagct gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg
    13501 gatgctgcta cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat
    13561 ttctttgaca acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt
    13621 caattttttt aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc
    13681 ttcatcacat aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg
    13741 tttttgtccc cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag
    13801 ttccagtagt tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa
    13861 agaaaaatca acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat
    13921 cataccgatg gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt
    13981 ctgattgaca atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa
    14041 cgtacttttc cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt
    14101 taaatctaca gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac
    14161 atctcgcaat tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg
    14221 aaaatgtcat tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt
    14281 tcacacacaa ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc
    14341 atcgccttac atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc
    14401 ttgcttcttc tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt
    14461 caatgcaaat aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag
    14521 gacgacgtat cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc
    14581 aaaaagagag attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg
    14641 acaaattatt ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga
    14701 gcctctctgc aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt
    14761 gggattaatc tacatgagga aaactgaaaa gtcaagtacg aaattcaaat tattttttct
    14821 atatttattt ttctgttttt ttatttaaca tttcaataac agttcgaaga tcttttcctt
    14881 ccgcaagaag tttcaatttt tccttttctt cgacggtata atttcggaca aaactggaat
    14941 caggtatcaa aacaacgcct aatcctgctc gtgaagcagc tgttgctcct actaaagaat
    15001 cttccactgc aaaggctgaa ctagcttcta atcctgttcg ttccaaagct gtcagataag
    15061 gaatcggtga aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat
    15121 cagctaattt aaagtgatcc aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa
    15181 tgcctagttt catatgacgt tcttttaagt ctgccaatat ttcttttgca tatggtttta
    15241 agtggatggc tccgcttgtc agctgttgat aaaaatattc ttcacgcttc gctcggactt
    15301 cctgtacttt ttcagggcta cctgctgctt tcactaaaat atcaaaactt tgtttccagt
    15361 tttttccgcc ccaagcgtca acgatttctt ttgggatatg gatatcaaat tgctgaaatc
    15421 cccaaagcca gccatcacga tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa
    15481 atattgcacc tttcatctat acattaatcc tttccaaaac aatacacata gtttagcacg
    15541 actggtccaa aaagaacaga agcaaagaac cagccttctc gtttataaga gttcttggaa
    15601 tcgacggata tagattttct tcacagctgt cacaagcgcc agataagcga tgactgtagc
    15661 aaataaccat aaccagtaat tgccaggaag tggcatcaag cctaattcag caccaaaact
    15721 tgtaaaagga aggacagaac cgacaataat tcctaatgta gtgataacaa acatggcaaa
    15781 tgttgcgttg ctttggataa atggtacttt cggtgtacgc agtgcatgga gcactaaggt
    15841 ttgtgtccat agtgattcga caaaccatcc tgcatggaat aatgcgatga aaatcactcg
    15901 ctgtgaacta tccaatgtat ggaaacttcc gcccactaca gcaggacaaa taacaaaata
    15961 catcaacaag taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa
    16021 ttccccaata ctagatgcat tccatttttt tggctcgtcc aaatattctt tatccatccg
    16081 atcccatggc aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa
    16141 aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa acatattgcc
    16201 aaaattagaa cttgctgtag ccttgatata tttcatgata ttcccaaaag tttctcgtcc
    16261 agaaagaagg cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgcaga
    16321 ttctttcgca atatccacag ctgtatctac agaaataccg atatcagcca ctttcattgc
    16381 aggagcgtca ttgataccat ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc
    16441 tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg aatacttcat ggtcttcagc
    16501 tacttgtgct aattcatgat cactcatttc cgagattttc tcaccggtaa ccaattcatt
    16561 gatctctaga cctacttgac ggcacactga acgtgtaact aattcgttgt ccccagtcag
    16621 aacttttaca gctacaccat gttctttcag agctttcaac gcttgtttcg ttgtttcttt
    16681 tggtggatca aggaaagcaa gataaccaat caaaaccatg tctgactcat ctttgactga
    16741 aaattctcca acgacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccatc
    16801 ttcattaagt gactgtactt ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt
    16861 aacagtaccg ccaagatcaa taaatttaga aatgttcagc atttcttcaa tcgcaccttt
    16921 tgtgatcatt tgtgttttac cagctggatc agcaaccaca acactcatac gacgacgttc
    16981 aaaatcaaaa ggaatttcat ccactttttg ataattgatc ttatctgtat ctaaagtttc
    17041 tgttgcttca tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct
    17101 atttagatac gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata
    17161 ttcaagaatg attttatctt gtgtcaatgt ccccgtttta tccgtacata acacatcgat
    17221 tgcaccaaag ttctggatgg cattcaaatt tttgatcact gttccttttt tggccatcac
    17281 acttgctcct ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gtgttagacc
    17341 aactgctaca gatagtccaa aaagaaaagc ttctagccaa tccccttttg taagcccatt
    17401 gatgacgata actgctggag ccatcaatga cataaatttg atcagtagca tggatgtttt
    17461 atggatgcct aattcaaagc ttgattgaat tggtttttca gatagcgttt ctgctacatg
    17521 accaaatagt gtttcatttc caacagaaac aatgattcct tccgcacttc cactgatgac
    17581 attacttccc ataaagacaa gattttcgta acttgtttcg ctttgctgat cgtcttcaca
    17641 tactttcgca tgcttttcta ctggatagct ttcccctgtc aatgctgctt gggaaatgaa
    17701 taaatccttt gcggacaata aacgcatatc cgctggtatc atatcccctg ctgaaagccg
    17761 aacaagatct ccacagacga tttcttccat aggaatctct gtgtactttc cattacgttt
    17821 tacagctgcc gttactttca caagtgattt cagtttttca gccgcttgat tggaacgtac
    17881 ggactgaatc aacgtcattg tcccgctgac gataaccata gtgaagataa tggctacacc
    17941 gaaaagatct ttatctccag gagctgctaa tacatattcc gtgatgaatg aaataattcc
    18001 taatgcgatc aacactaatg taaatggcgt gatgtatgct ttcaatattt ctacaatgaa
    18061 tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat
    18121 ttgttcttct gtcaatccat ttgctgatgc atggaatagt tcaaataatt cttgtttcgt
    18181 ttttccagca aaagctgcat agattttttg tgtatctttc tttttttcca tctcaatctc
    18241 tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg tctttagcta ttcatcggat
    18301 cgatagagaa aaaactgaaa ggggctgaga acagatagat aatagccctg gcctttcctc
    18361 tagatccttg tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat
    18421 tttaattcat gacttccaat catttttcga atagttattt ttccaattag accacctcct
    18481 ttttattttt caccgatggg gatttgcggc aacaaaaaaa cctctcgcca aatactcagc
    18541 gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg agttttgaca
    18601 ctgtacaacg taaaaagcag atacctgctt ttagctacct tatgaaaagc cttatctcga
    18661 caattcctgt tctacccatt ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta
    18721 taggagcctc acctaacaaa tcttattcgg ttttccttcg ttaatataac aactaaaaaa
    18781 gaggatgtca aatcaatttt ccatgttttt catacttttt ttaaaataca cacgttatat
    18841 tcctgtctca cgcggtttta tttgtctcat tttccacctc ccgctataat agaaatatag
    18901 taggaaacga ggtgctgtta tggaaagaac acttgttatt atcaagccag acggcgtgag
    18961 acgccattta gtcggaagca tcatccaacg ttttgaagca aagggactgg ccattgctga
    19021 aatgaagttt gaaactatga ctcctgaatt agcaaaagaa cattatcagc acttgactga
    19081 gcgcagtttt tttgatgaac tgatcgatta catgacttcc ggtcctgtcg tttatcttgt
    19141 gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc
    19201 cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg ccggggactg aaaacatcat
    19261 tcacgcttct gattcccgtg atgctgcggt aaaagagatc gcccgctttt tcccgccatt
    19321 cgatataaac aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa ctgctggtct
    19381 agttgctggg ttcagcaatg gccagcagtt ttttctctaa ggacctccgt tgattctttc
    19441 ctaacatata ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt tcttccccta
    19501 aagcagcaga aatcaatctt tgataataat ctgccatcaa tgtttcatag atcactgtac
    19561 ttctttcttt atgtgctaag ctccgtttca tatcttcatt cacactacgt gcctttttca
    19621 accgaccatt ttctatataa aacgaagtca ggttcattaa aaaagtaaag agatactgct
    19681 catgattgtt caactgtgct aatcgtcgca ttcttttttt gattttcaaa taattgttct
    19741 caatgaaccg atcactgaat atccaaggaa aatcagctag aatagatagt tcaaattgac
    19801 catagtctgg tatctgcatc aagtattctt caatgattcg gcattcataa cgtaaaaatt
    19861 caaattctgt ctcagatact tctgtttttt cgaccatgtg aagattcaaa accaaataat
    19921 acagatcttc tgttttctta aaatgcctac ggttattgct gatctcttta taaggatcaa
    19981 agctgtcttc ttgtacccga tctagcattc gtcttttttt ctttaacttt attggttcat
    20041 ccagctggtt tcgcatccat tcatattctg caagtgtcat gttcattcgc tctaaaaatg
    20101 atctcaatgt tacaaatgac atttttttca ctgaatttgt ctcaatcccg actagagaag
    20161 tcttaaaaca gattccttcc gctaattggg actgtgtcaa cttcttgttg actcgaagtt
    20221 tcttgatcaa ttcaccatca tacataatag ttctcctttt taatttaaat ttttccacat
    20281 tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt
    20341 cataaaaaat accaaccttc aagcattaga atgttgtcta actactgaag gttggtttga
    20401 ttgtttattg attatttttt tgctgttcaa cgtctgttct tgttactgga tataagttgg
    20461 ttgttgtttt attaccattt tttgaaaaga cacttgtcga atgttcgcct ttttgctttt
    20521 cgattgcttc attacgttcc gcttcatttt tatagtcata ttttttcgga tcaatcgtct
    20581 caaatccgtt tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac
    20641 ttaacgcagt cttgactttt tcctgatctt tggagatttc ctcatcgatt cctgctgctt
    20701 tcgtatcgat gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt
    20761 tgggtgtcac aaagttccca tttcgaaaag caactacttg atcatgctga gaagataata
    20821 agtctgtacc aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg
    20881 gcaaaacatc gatttctcca ccgtattcat gattgactgt ccctttagta taaccaggca
    20941 tatggatcat caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg
    21001 gatctttacc aagagcgctt gctaaatctt tattatctgt attcgagatc ccaaaatgat
    21061 ccccgtaaat cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa
    21121 attgttccaa tgattgatcc agataatgtg ccgttcgaac ataattatct actgtgctat
    21181 tgccagtatt caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt
    21241 tggttacgga aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga tattgggcag
    21301 attctctgaa taaatcttta tctaatatcc cataacctaa cgtttcgtcg gattcatcaa
    21361 aataagatcg atcaaagaaa ttgtcataac ctaagttttt atatacatga tctctattcc
    21421 aaaaactagc tacattccca tggaaaacag cgcttgtata gccttgcttt tgtccaagga
    21481 tcgctggtgc ggcttgaaag acattgtctg acccaagttc tgtgaataat gagccttgag
    21541 gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc acttgtcttt ccctgtccta
    21601 cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa
    21661 acggtgtgac ttcttgtccg tctaccttca tattaatcaa aaactgctgg aaactttcta
    21721 aatggagaac gatcacattt ttccctttag cgatcccaaa tgtttcggga tttggctctg
    21781 cataatgttt ctttgtataa tctaaaactt catcaatccc attactgctt gcatgcgcgc
    21841 gaacactgct agtcattcct gttttgattc cgtcataaat agtatacgca tcgattccta
    21901 gatatttgac aatatatgaa cggtcaaatg ttcgctgcag caattgtgga cggttggctt
    21961 cgctcagcgc tagatttcct gaaaaaataa gccctgaaag acaggtaaca gcgatagcca
    22021 tcggtttgcc gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta
    22081 gcccaatgaa cacagcgata tccagccagt agatgacatc atggggcttc atcaaagaaa
    22141 aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc taatacagat ttgactgtaa
    22201 tgaaatctgt gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa
    22261 tatcaatgat caaaagaata gctaacgctg gctttggctt tttaatatac aaagaaaggc
    22321 caaaaaacaa taaggtagtc gcaattgggt tgatccacaa gataaagtac tggatcgtac
    22381 cttccacacc taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga
    22441 aaacagctgt caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt
    22501 atatcacttc tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct
    22561 aaaaaagttg ttatcttaag atttttttat tctttctaaa gattattccg tccacttttc
    22621 acagcataaa accgctgcga tgtaagcgat taaactaatg aagagataaa agtcttgtgg
    22681 tgaccgcttc actccacggt attcaaaagc cagtttctcg gtattcgtac aaaaaaagag
    22741 gctgtgacaa aattcttgtc gcagctcctt atttcttgat gcaggacagc tttttcacaa
    22801 cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca atatttataa
    22861 aacatgagaa gctgtttttt gaaattgttc cttgtggctc agagttaaac ggcaggctca
    22921 agacctcttg atacacggtg ttcaatcagc tgaccctcgg tattagatta tgtcaggacg
    22981 ctaattttac aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    23041 aaccaaacga aaaatataag gagctatttt ttgaaattgc tccttgtggc tcagagttaa
    23101 acggcaggct caagacctct tgatacacgg tgttcaatca gctgaccttc ggtattagct
    23161 caaaaaacgg caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa
    23221 aacgctgatt tcacaacctc tgtttttcat tgtccatagg attgatattg tccttgtgat
    23281 gactcattcg taataattga ggcttgattt tcttcacttc ccgttatcgt ttgtccactg
    23341 ccagaatctg aaggacttgt tcctgtcgac tcgttttttt gagaagagac agttgttttc
    23401 ttgacttgtt catttgtctt tttccattcc gctaaacgat cggcgaccac ttttttgtct
    23461 actttttcag aataagcggt gatcactttt ccatttttca aacgggctac tttgtcgcca
    23521 tcatgagtca aaagcaaaac ggtcaaatgc ttgtctttaa attgatattg tctgagagaa
    23581 atcgcactaa gtacgtcatc gatattaata aattgatttt cttcttttcc ttttggcaaa
    23641 taaatgtcca attcgccttt ttcgtaattg gcagttattt tatactccgc ccaccaataa
    23701 gttgagaatt cttttttgaa attatctact gattctttgc ttgctacgat tttttctgtt
    23761 tctttctgag caagatctgc tgtcattttt cctgaaaatg ctgcggataa aataagcagt
    23821 aagaaaaaac cgaataacca aaagagaaga aacggtcttt ttttcctcat gctttcctcc
    23881 actcctattt aattttgact attctattat atacaaaaaa tgaaatagaa agacaaaaaa
    23941 gttgaaaaag atcgtaaata attgaacttc tttttcacaa aatggtaaga atggagaatt
    24001 tcacgcattt gctctcaact attctttctt aagcctcaaa tccgttttta tctgatacgt
    24061 ctttgaacca atgacccgat tttttgattg ttctttttcc atcttgtgct aagtctactg
    24121 aaataaagcc atatcgattc ttatatgcat tggtccacga ccagttatcc atacatgtcc
    24181 acatatgata gcctaaacaa ttggcacctt cttgaatggc ttgatggaca tatttcagat
    24241 gagattggac aaattcgatc cgataatcat cgtggatcat gccatcttct ttgataaatc
    24301 gtgcttcgtt ttctacaccc ataccgtttt cagaaataaa gcagtcgata tttccataat
    24361 gatctcgtac attgatcaaa atatcataga ttccttgttc atagatctcc catccgcgat
    24421 aaggattcat tttacgacca ggcatttcat aattgtcgaa ataatcctca ggcattggac
    24481 cgtttttagt atcgattggt gtttcttttg cttttacccg gcgtggctgg taataattga
    24541 tacctaaaag atctactgta ttttctttga taaccgtcaa atcctctgca tggatcacag
    24601 gcatgagatc aagttctttt actatctcaa tcaattcact tggaaactcg ccttttacag
    24661 ctggatcaag gaatgaacga ttgaagaacg catctgctat ttgcgctgct ttaacatctt
    24721 cagggtttga ttcgtctctt ggatagcttg gtgtcagatt caagatgata ccaataacac
    24781 caccttgctc catttcatgg taagatttga tggctaacgc tgaagccaac gcttcatgat
    24841 agccaacttg gatcgctttt ttcatatctg actcttctgg ataatgccat cgatataaat
    24901 aacctgcttc gactggaacg attggttcat tatgagtaaa ccattttttt actttcgaac
    24961 caaacaattc aaaacaactt ttggcatatt tttcataggc ttcaaccgtt tctcggttca
    25021 accatccgcc tttttcttgc agatacatcg gcatatcgaa atgatacaga ttcacaaagg
    25081 gttcgatacc gttttcaatc aactcatcaa aataacgatg atagaaacga acggcttctt
    25141 cgttgattgt tccagattca tcaggtatca aacggctcca ttggatagat gttcgaaatg
    25201 aattatgccc agtctgtttc atcaactgta catcctcttg atacttcgta taaacttgcg
    25261 aggttttttc aggtcctact tgatcaaaga acttttgcgg ttcttttttg taccaataat
    25321 caaaaatgtt ctctcctttt ccatcgcctg aaatccttcc ttctgtttgt gggccacttg
    25381 ctgcagaacc ccaccaaaaa tttgctggaa attgatattt tttcatcaaa gcacctcttt
    25441 ttctttttta gttcgtttta gttgattcca tgtatgatat tttccatcac aatcactttt
    25501 tcattttctt cctcttcttt tttgattcgt tttaacagct gttctgctgc tcgttccccg
    25561 attttatctg ttggtggcgt gatcgtgatt ttcggaagga tactcagatc gtttttatct
    25621 aaataatcat agtgactgaa cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa
    25681 cattcgatgg caccaatcga catattgtaa ttgctgacaa aaatagcagt tgcccctttt
    25741 tctaataatg cttttgcccc gtaatagccg ctttttctag aatagtctcc tgtaaagacc
    25801 tctaattgga tcgatggatc attttggacc gcttctttgg ctcctgctaa acgttctctt
    25861 gctacgtagt ctgtttgagt ggcagcaata aatccgattt ttttatgtcc ttgttcacca
    25921 tgatgagcga tcacttttgc tacactttct cgatcattca ctaatattgt atcaatacct
    25981 gctggtcctt ctggacaatt caatgcgata actggaatac tcatttttgt gagcatttca
    26041 aatccagacc actcttcttc ggataagaag acaatcagcc catcgatcac atgcttcatc
    26101 aggtattcaa ttttttgttc aattttttct ggctgatcgg caaacccgct taataataag
    26161 ctatacccat tttcttcagc tactctttcg atccctgaga caacttccga cccaaaacgg
    26221 ctggaaatcc cgtttatcaa aagtccaata gagaagcttc gattattctt caacccttta
    26281 gcaatgatgt tttctttata gtccaacgct gaaatcgctt ctgctatttt tttcatgttc
    26341 ttttccttca gttgctgacc attcaagtaa cgggaaactg ttcccactga aacaccagcc
    26401 atcttagcca catcacggat cgatgccata cattctcact cctgccagtt acatttgatt
    26461 tctctttctt gttgttcgat tatgatgacc gcattctttg catagtgttg cttgtcatca
    26521 atatccatat aactaaagtc ttcagtagag caacttcctc tcactattaa ttatacttaa
    26581 acaagtattt gaaccggttc atttttaata tagcattttt ttaaaagatt gcaacacttt
    26641 tttaaaacgc tttctgaaag tggcggattt tttgcctttt taaaatttta ttctaaaatt
    26701 tactctattt gtgggtgttt tgtgcgtttt tctcttccat ccgtttgaag ttttacattc
    26761 caatggtata atttctttat cctatcaaat gggggaagta ttttgaagaa attaatcgta
    26821 ctatttattt tgtctgcggg acttttgaca ttgactggct gtgatttgtt tggcggctct
    26881 gaccaatcct cggacgaaac aaccgattct tctgtcttaa aagcggaaaa agcagatacc
    26941 accaacagtg tatatacgct aaaagtacgt aaagatatgg atttatccgc gggacagttg
    27001 acatttacag ataaaaaaat ggaatggaca cgcacgtatc agacacaagg aactgattct
    27061 tctgaatcat ctgaagaaaa aacagtgctg acagatatca agatcactac caaagatgac
    27121 gaatatatta tttcaggaaa agaaaataaa aaagaaacaa cagtcatatt tacaaaaatc
    27181 ggtaattacc gtttgaagga tgaggatggc aacatctaca gtctatagtg ccgtctgttg
    27241 gatcttcttt tgtttctccc cttcttttct gctatagtaa agaagcaaga tgattgtaaa
    27301 ggagacgaaa tggatgatcg atagtcctga ttattgtaca tgtaataaca acaaaaatat
    27361 ccatacagaa tttaatgacc aaggaagctt gagcatatgc gacaactgtc acaaactagt
    27421 caaagattca tttaaaacat ttgaatctta tcctgttcaa aattatgtaa aaagtagaag
    27481 aagataaaca aatccaagca aaactcacta tggctgattt ttatgaactt caaactcagc
    27541 ttcttccttt gatcaagctt catcagtaaa acaaacagtg gatcatttca gtttacaaat
    27601 caaagagtaa agatttattt atttcaaata tttgaatata aaataaccaa acgatagaaa
    27661 ggcttattct acatcacgta gta
//
LOCUS       DAZKOB010000024        27515 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000024 DAZKOB010000000
VERSION     DAZKOB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.024"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            293..1462
                     /locus_tag="ACG8M6_001063"
     CDS             293..1462
                     /locus_tag="ACG8M6_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0456749.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1935..2489
                     /locus_tag="ACG8M6_001064"
     CDS             1935..2489
                     /locus_tag="ACG8M6_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0456750.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2662..3099)
                     /locus_tag="ACG8M6_001065"
     CDS             complement(2662..3099)
                     /locus_tag="ACG8M6_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0456751.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3147..4094
                     /locus_tag="ACG8M6_001066"
                     /pseudo
     CDS             3147..4094
                     /locus_tag="ACG8M6_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4365..4800
                     /locus_tag="ACG8M6_001067"
                     /pseudo
     CDS             4365..4800
                     /locus_tag="ACG8M6_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4816..5649
                     /locus_tag="ACG8M6_001068"
     CDS             4816..5649
                     /locus_tag="ACG8M6_001068"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0456752.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5894..6919
                     /locus_tag="ACG8M6_001069"
     CDS             5894..6919
                     /locus_tag="ACG8M6_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0456753.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7057..7182
                     /locus_tag="ACG8M6_001070"
     CDS             7057..7182
                     /locus_tag="ACG8M6_001070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456754.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7207..8322
                     /locus_tag="ACG8M6_001071"
                     /pseudo
     CDS             7207..8322
                     /locus_tag="ACG8M6_001071"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8405..9301
                     /locus_tag="ACG8M6_001072"
                     /pseudo
     CDS             8405..9301
                     /locus_tag="ACG8M6_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9317..10141
                     /locus_tag="ACG8M6_001073"
     CDS             9317..10141
                     /locus_tag="ACG8M6_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456755.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10173..11483
                     /locus_tag="ACG8M6_001074"
     CDS             10173..11483
                     /locus_tag="ACG8M6_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0456756.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11631..12890
                     /locus_tag="ACG8M6_001075"
     CDS             11631..12890
                     /locus_tag="ACG8M6_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0456757.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12900..13772
                     /locus_tag="ACG8M6_001076"
     CDS             12900..13772
                     /locus_tag="ACG8M6_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456758.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13784..14614
                     /locus_tag="ACG8M6_001077"
     CDS             13784..14614
                     /locus_tag="ACG8M6_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456759.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14654..16837
                     /locus_tag="ACG8M6_001078"
     CDS             14654..16837
                     /locus_tag="ACG8M6_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0456760.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16898..18118
                     /locus_tag="ACG8M6_001079"
     CDS             16898..18118
                     /locus_tag="ACG8M6_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0456761.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18115..19575
                     /gene="gtfA"
                     /locus_tag="ACG8M6_001080"
     CDS             18115..19575
                     /gene="gtfA"
                     /locus_tag="ACG8M6_001080"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0456762.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19652..20758
                     /locus_tag="ACG8M6_001081"
                     /pseudo
     CDS             19652..20758
                     /locus_tag="ACG8M6_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21080..21289
                     /locus_tag="ACG8M6_001082"
     CDS             21080..21289
                     /locus_tag="ACG8M6_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0456763.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21282..21431
                     /locus_tag="ACG8M6_001083"
     CDS             21282..21431
                     /locus_tag="ACG8M6_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0456764.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21585..22250
                     /locus_tag="ACG8M6_001084"
     CDS             21585..22250
                     /locus_tag="ACG8M6_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0456765.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22534..23619)
                     /gene="xerS"
                     /locus_tag="ACG8M6_001085"
     CDS             complement(22534..23619)
                     /gene="xerS"
                     /locus_tag="ACG8M6_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0456766.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23953..24186
                     /locus_tag="ACG8M6_001086"
     CDS             23953..24186
                     /locus_tag="ACG8M6_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0456767.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24183..24590
                     /locus_tag="ACG8M6_001087"
     CDS             24183..24590
                     /locus_tag="ACG8M6_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0456768.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25727..26245)
                     /locus_tag="ACG8M6_001088"
     CDS             complement(25727..26245)
                     /locus_tag="ACG8M6_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0456769.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26612..26860
                     /locus_tag="ACG8M6_001089"
     CDS             26612..26860
                     /locus_tag="ACG8M6_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456770.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            27363..>27515
                     /locus_tag="ACG8M6_001090"
     CDS             27363..>27515
                     /locus_tag="ACG8M6_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0456771.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         9425 a         4179 c         5082 g         8829 t
ORIGIN      
        1 ttaataatat actaccattt gttggtagta taatttaaag gtaataagtt attcttttat
       61 tactatgttt gggttttata gcaaagaaag tttaataaga gtttggacct agcaaacaat
      121 attaatgagg tcattaaatc ttattatctt tagtgatata acgaaatatc tacacagaca
      181 aaatgatagc gtaaaatatt ttgtgtaaat agatatttag ggtagaaaaa aagaaagtcc
      241 attctgtaaa attaaagtta ccacaaccga aattttatag gaggactttc tcatgcccaa
      301 ttttactaca gaaattatgg aaacactaat caataaaggt gatttagatg aattatttcg
      361 atgtcattta gaattagctg ttaattcatt attgcaggct gaattaacag cttttcttga
      421 ttatgaaaaa tatgatcgtg ctgggttcaa ttcaggtaat tcccgtaatg ggaattattc
      481 acgttcattc agaacagagt atggagaatt aaatttggta attcctagag atagaaatgg
      541 taagttttct caacaaactt tacctgctta taaaagaacc aatgactctt tagaaaccac
      601 tattattcag ctatttcaaa aaggaattac tatgtctgaa atctctgaat tgattgaaaa
      661 aatgtacggg catcactata cgccacaaac catttcaaat atgactaaaa tagtttctga
      721 agatattatt gcctttaaag aaaggtcctt agaatccaga tactctgtca tttttatgga
      781 tgctactcac attcctttaa aaagacaaac tgtttcaaaa gaagcagttt atattgtaat
      841 tggcattcga ttagatggaa ccaaagaggt tcttgggttt agtcttgctc caactgaatc
      901 tgcttatgtt tggaaagaga ttcttcaaga cctaaaagac cgtggtttaa aagaggtttt
      961 attagtcgta actgatggtt taagtggcat taacgatagt attcatagtg tttatccaaa
     1021 tgctcaattt caacaatgtt gtgtccatat atctagaaat atcgctcata aggttcgtgt
     1081 tagtgatcga caagaaatct gtagcgattt caaattagtt tatcaagcaa gttcaaaaga
     1141 agaagcaaat aatcaaataa ggtttatgat agataaatgg aaaaagcagt atccacgagt
     1201 ggttaaattg ctcatgaatc ctgctatatt aaccttttat aatttccctc cgtcaatcag
     1261 aagaactatt tattcaacta atttgattga aggttttaac aaacaattaa agaaatatac
     1321 caaaagaaaa gagcaatttc ctaatgaaga atctctggag agatttctag tatctcaatt
     1381 caacaactat aatcaaaagt ttctatgccg agttcataaa ggttttaaag aaatacatga
     1441 tacattagaa tcaatgtttt aacttgatca acagaatggg cttttccatt tacacaaaat
     1501 tattgacgct cccgacaaaa ttatgatctt gcgtaaagtt ataaaatatt aataaaaata
     1561 ttttattaat gaaaagcttg gtagcacggt tattcatatg ctaatttatt tcactttata
     1621 gtaaggggag ttcttagtat attgttcaaa cttaaatttc atataattta attatgtaaa
     1681 ttagctattc taagaacttc tacggataac tgcccgtcta aaatttaaat aagagcttag
     1741 aatcttcata taagtaaggt taattttttt gtacaaaaaa gtcggaatat tctgagttac
     1801 tgaaatgata taaaatcata aactaaatcc cttgatttta ttgactttca aaagtttata
     1861 tatataagtt aatgattatg gtaaaatata tacataatca ttaactcaga atatttttaa
     1921 gaagggaaac aatagtgagc tataatgtac aaccgttaag gacaaatgaa gagatacaag
     1981 attttcagtt ttggctaaga agaacaagta atccggaaag agatagtttt ttatttttgt
     2041 ttggaatcaa taatgggtta cgtatgtcag atattatagg attaaaagtt ggagatatac
     2101 gaggcaaaag taagcctatt attgttgaac ataaaacagg taagcgaaaa ccgattttta
     2161 tagataattt gagagaagaa attttattat atactgaagg taaagaagaa aatgattggt
     2221 tatttccgag tcggcaacaa ggtcgacata taacaaggga tcgtgtctat cagatatatg
     2281 cagatatagc agaaaaatta ggacgagatg atataggaac acatacatta agaaaaactt
     2341 ttgggtatca ctactacaag aaaactagag acattgctac cttaatgttt atttttaatc
     2401 atagctcgca agcaattaca aaaaggtaca ttggtataac tgaagatgaa ataggagcat
     2461 cacttcgtgg atttaaacta ggtgtataat ttaaaaattt ttataaacag taaagacaaa
     2521 gattaaaagc tatttttatc tagaacgatt ttatttcata aagttcgcgg aataaaagct
     2581 ggggttactg gtacttggta taggttcgtg ttatataaac taagtaatta aataacattt
     2641 ttaggttgga aaaggatgaa attaaaaaaa gagaatcaat acggtctcaa aaacgcaaac
     2701 gctgcggaat aagaaaaata ctgataactc aacgtttagc agactatcta atagggatag
     2761 tctgcttttt tcgtttttga ccatactttt gaccttctgc aaacttcctt gcttttcctc
     2821 tgtagaactc atgaaatctg caaaacgaat tgccgtttcc tctgtctgct ttttcgcgat
     2881 atgggtgtat atatcatcca tagtggtttt aatatctgta tgtccaagtc gaacatacac
     2941 ttctttgaca gacgcaccag attcaaagag cagactacaa tgggtatgac gaaaaccatg
     3001 aagtgtgata tgttttaagt tattctcttt aatgatgatt ttcaaattgt gatttacata
     3061 gtctaaataa aggggcttat tttcttctga tgaaaacaaa agttgttcac catcttttat
     3121 aggtttgatt ctcattttta gatattatgc ccgcacgtat tcatcacatt tctattgtga
     3181 atcgctttat tcgaccgaca tttgactttt atcataatat actcggttta aaattactaa
     3241 tgaaaacaat taatcaagac gatcatacca tgtatcacct gtttttttca gataatcacc
     3301 accgtgtggg aactgaactg actttttttg aagtacaaga agggaacaac cagttttttg
     3361 gaaccaatac gattgaacga acgatactaa aagttccttc cgaagcatca ttgcattttt
     3421 gggaaatata ttttgaaaca caaggcgttt gccattatgg aatcgaatca ttttctggac
     3481 gacctatctt acgttttgaa gggccagatg caacacgatt agctttagtt cccttacgcg
     3541 aatttgaaga tattgatgat tatttcccgt atgtcacaga ccagatccct acagagcatg
     3601 ccattttagg aattgatgcc attcaactac gcgtacaata ttccgatgcg actgaacgag
     3661 agttattgtc ggtaggctgg tagcacaaag aatacgtcag tttcttttcg ggtcatcaac
     3721 gagtgacaat cttagaaaat caagacactc agttctatca agaagttcag attattcaag
     3781 atcgtgagaa tccagttgcc attgaaggga ttggtagcgt ccatcatgtt gcgtttggcg
     3841 tagaaaataa atctgattta caacgcatcg acaaacaatt acaagagcgc aattttataa
     3901 attcaggaat aaaagatcgt gaattttttg tatcacttta ttatcgtgac ccaaatcaat
     3961 tattgattga aattgctaca ggggaaggaa acctagatgc aaaagcgtat gaaaatcaaa
     4021 gtccacgctt tgaagaaatt cctttatttt taccgcagta cttaaatttc attcgtgaac
     4081 aagtggagca ataattagct aatcaaatcc atgacgaaga gtaagaggtt gactcataag
     4141 tcaaaaaata aagaaaaacg catcaaatcc attctaaaga atgttgattt gatgcggttc
     4201 ctatttttat aaactatcat tttcgactaa taaaactact tatgagtcag cctctctttt
     4261 tttcttgatt tttatacctc tttctaggta aactaaacat ctgccaaaaa taccgtgtct
     4321 cattgaaaca ttctcaacta tttcaaaata aaggggagta ccaaatgaac gagtatattg
     4381 aaattatcca cgcaagagaa acaacttaaa aatgtcagtc tcaaaattcc caaaaataaa
     4441 attagtattt ttaccagcgt atctggttca ggaaaatcat cgattgtttt tgatactatt
     4501 acccaagaag ctggtcgtca actgaatgac acctatgata gttttacccg tttattttta
     4561 cctaaataca gtcgtccaga ggttgatgag attgataatc tttctacggc gattgtggtc
     4621 aatcaaaaga agattggtgg cagtgcgcgc tcaacgttag ggacgattac taacatcaat
     4681 gcgttatttc gtgtgttgtt ttcatgtttt gcaacaccta gtttaggata tgcaaatgct
     4741 tattcattta atgatcctag tggaatgtgt tctgtctgtc agggaatcgg taagacgatt
     4801 gcatttttaa taggattgct ttatttttcg gctattgata atgctgattt tgtattttct
     4861 gatccattta ccgcagaagt tttgtctgga attgaggaac aggcaaagaa tcatggttat
     4921 tttgtattgg tgcataatgt taattcagca gaagatgtta aaactattca gagaaattgg
     4981 cgttttgagg ggttcattgt tgttggtgta cgtgtaaaag aatttttgga attaaataac
     5041 gtttttgatg gtcctgttgt ttatattgat acgcatcttt caaaagagac attaagagaa
     5101 cttgaacaac aaatgaatcg tttatttgta aatacaaatg attaccaagc tagtacattg
     5161 gcagtggaac atttgattca ttatggtcat gagaaaatag cgtttctttc ttttgactac
     5221 attgtaggtg aaccaagtgt tattcaagaa cgacatcaag cttatatcga tacattaaag
     5281 aaatatcatt taacctttaa atcgcattgg atatataaca actctgaatt tgagcgaatt
     5341 tatcaagaat tagctgaatt tactgcaatt gttgttactg gagattattt agcggctaaa
     5401 ttagtaaaat ttttaaaaaa taagaataaa cgagtgatgg aacaattatc gattattgga
     5461 tttgatgata ttacttttgc agaattaatg gatcctgcgt tgacaacaat acgactaaat
     5521 ccaggtaaca agggggcgat tgcttttcac caactgattg gactggccaa acaagaagaa
     5581 ataaaaaatc aagtactact attagaggga gaattgattt ctcgagactc tgttcacaaa
     5641 atcaaataaa aaagctctgt gaaataagta tagttaacaa taaaatatcc gaacgataga
     5701 gagaatgaac tgtgatctag cagttcctcg tcgcaaattc actagaatta gcattattta
     5761 ctctaaattg ttcggatatt ttttcttgtt tttaattatg attacagtgc ctatttttaa
     5821 cgaaatttgc ttattttcaa aatgtatgat tatatgttag taaacacttt caaatatgaa
     5881 aagaggagct attatggtag gaattaggga tgttgcaaaa cgggcgggtg tgtccattgc
     5941 aacagtatcg cgcgtgctaa atgaggatcc gacactttca gtaaccgatg aaaccaaacg
     6001 aaagattgca gaatcggtta atttttatca ttatgtcaaa aaaagtcccg ttgcaaaaaa
     6061 tttaacaaat attgcattaa taacgacagt ttctgaagta gatgagttgg aagatcccta
     6121 ttttcgaacg attcgcagag ggattcaagt cgaagcagaa caaagcaaag tcaatttgaa
     6181 gaaaattatt cgtttgtcag aaacaccgtt ggagcaagac gatttgctaa aatgtcaagg
     6241 tgtgttgatt attgggcagg tgctatcttc tgtgattgaa gaagtatcag caattaattc
     6301 gaatgtcgta gtgattgatg accctagtat tgaagctgaa gtagatgcag tttatactga
     6361 tttaacaaaa gcaacgaatc agcatttaga tcgattgtat aaaaaaggac accgaaacat
     6421 tgcgtttatc ggaggaggac gtgttcgttt agatagccaa ggaaataaac acgtgtcaga
     6481 tgatgattat cgaaaaatag cctatgagac gtggatgaaa caaaaaggat tagaaaaata
     6541 tagtcaaatt tatttagaag gctggaccac acttgacgga atgcaggcag cagatttact
     6601 tttgaaaaaa catgctaaag ataagttgcc gactgctatc atggtaggaa atgatcctat
     6661 tgctgtgggg gtttatcgtt cacttcaaaa aaatggtcta catattccag aagatatttc
     6721 gattgtgagt tttgataata ttgaagttgc tgaattttta acacctgcat taaatacggt
     6781 gaatgtgaat acagaagaaa ttggaaaact agcagttcgc atggttaatg aacgaataaa
     6841 agaagttaga aatgtttcga tcaaagtgat tgtttctaat aatattattg ttagagaaag
     6901 tgaaaaaaat atcgattaaa aaagtaaaca tccaaaaaag ggtgtttact tttttgttta
     6961 ctaatatttt tagtaaaaaa tctcgacaaa acattaagta aacagttatg gtgttgttaa
     7021 caaaaagaaa acgcattcaa aacaggagga attaagatga aacatttcaa aaaaatagct
     7081 ttaacaggga tagctgtttt aactgggaca ttactcgcag catgtggtgg gggagatagt
     7141 aattctaaag acgggcaagt aactttaagc tatgcgagtt gagatactga ccaagctcca
     7201 ggactaagaa aaatgttaga tgaatttgaa aaacaaaatc ctgatatcaa agtagaaatg
     7261 gagacaacgc cttgggatca atattgggtt aaattagaag ctgcaacaac gggaggtaat
     7321 atgccagatg tagtaaccat gcactctagc gaatcctata aatatatgtc acagaatgct
     7381 ttgatgggac ttgatgatgt tatcaaagaa aatgatattg acatgggaaa ctttactgaa
     7441 ggaattgctg atttctatac gcttgaagac gaactatacg caatccctaa agatgcttct
     7501 gtagtggggc tatggtacaa caaagaatta tttgataatg caggtgtcga ttatccagat
     7561 gaaacatgga catgggaaac actcagacaa gctgcaattg atttaacaga tgaatcaaaa
     7621 ggcatctatg gttttgcatc acatattgga acagagactg gctactggcc atttatttat
     7681 caaaatgggg gagaagtatt tgccgatgac aatacaaaat caggtttgaa taccccagaa
     7741 gcacaagaag ctttagcttt ttatcgagat attgcacttg ttgatcatgc ttcaccaaca
     7801 attacagaat tacaagatcc tgataagtta agtcgttttc aatcaggtag attagcaatg
     7861 atgattgaag gaaactggtt tgcaccggga tttttgaaaa atgactatct agttgaaaat
     7921 gttgcttctg ccgtgttacc taagggaaaa gttaatgcaa caattacaaa tggacttggt
     7981 tgggctgcca gtgcgagtac aaaataccca gaagaagtga aaaaactaat ggcattttta
     8041 gctagtgagg acgccaataa aatccaagcg gaaacggggg catcaatccc agcactaaaa
     8101 ggctttgaag acgagtgggc tgcttcttca gatacaattg atttgagtgc ttttggaaaa
     8161 atgttggagt atggagtccc tcgtccatac aataaagatg gattacgagc tgaacaagca
     8221 gaagttcaaa ttattaaccg tattctgtct ggtgaagtaa ctgttgaaga tggaaccgct
     8281 caaattgaaa aagaagtcaa tgcgatattg agcggaaatt aaattagtta attatacaat
     8341 atacaggcga aaggattcta tttcgtctgt atattaatgg atgaatacca aaaaggggga
     8401 tattatgaaa aatcgaattt ttggaaaata tcgtaaagat caagtgatat ggggctatat
     8461 ttttattttt ccaacggcat taggattaat tttattaaat atatggccag caattcaaac
     8521 agcaatttta agttttcaaa aaacggtagg gttcggtgat actgaatggg tcggattaag
     8581 aaattaccaa aaaatgtttg cagacggtga agtttttcag tctttattaa acacattaat
     8641 ttatgcagtg gtttcgatac ctagtattgt tgtattatcc ttgttggtgg cagttttaat
     8701 gaatagaaaa gttaaaggat tgtcgattta tcggacaatt tattttctac cagtcgttgc
     8761 tgcaccatcc gcagtcgcaa tgatttggcg ctggatgttc aataatgatt atggactgat
     8821 taataacatg ttggctaaaa taggtctcga tggaccagca tggttaacgg atccatcgat
     8881 tgcgatttat tctattatca ttgtaggagt ttggagtgct attggttata acatggtgtt
     8941 attactagcc ggcttataag aaattcctaa agattattat gaagcagcgg aaattgatgg
     9001 tgctagtcca atccagcagt ttttcaacat tacgttacca ttagttacac caacattatt
     9061 ttttgtagtt gtaacaacag ttatcaatgc tttccaagta tttgatgtta tttttatgat
     9121 gattactcct aatagtaccg ccatgtttaa atcacaatca ttagcctatt tattctataa
     9181 acattcattt attttaaatg acaagggata tggttcggca atcgttatgt tccttttagt
     9241 gattatatta attattactg caattcaaat gaagttgcag aaaaagtggg taaactatta
     9301 aggaggctat caaattatga aaaataaatt tttttcaaat gcgccaatcc atatcatgct
     9361 tattttaggt ggtattgcaa tggcattacc ttttatttgg atgattttaa cttcagggaa
     9421 aacgttggtt gaatctaccc aaattccacc tacaattttc cctgagaaat ttcagctaag
     9481 taactatcaa caagtacttg atttattacc attcatggct ttttatatca atacattttt
     9541 aatggttgtt ggacgagtga ttggctcggt tttattttca gcaatggctg catatgcttg
     9601 tgcgcgtcta agttttccag ggaaaaatat ccttttcagc ttagtactta ttcaaatgat
     9661 gattcccggt tccattttta ttactccaca gtatttatta gcacaaaaat tgggtatttt
     9721 aaatacaatt ccagcattaa tattaccagg ggtagttagt gcgtttggta cgtttttatt
     9781 aagacaattt ttcttaggtt tacctgatga attggaggaa gcggcctatt tagaaggtgc
     9841 caatatttgg acgatctttt tcaaaattat gttgccgctt gctaaatcag gattggttgc
     9901 acttggaata ttcacagcgt tgtttgcatt taaagattta atgtggccat tgattgtcaa
     9961 tacttcaatt gataagatgc ctttagctcc gggattagca aacttacaag gacaatatgc
    10021 cacgaacttc ccacaattaa tggcaggttc gttattagca atttggccaa tagttgtgat
    10081 attcatcgtt ttccaaagaa aattcattga aggaattgct acaacaggta gtaaattata
    10141 aaactattaa ttagtgattg gagagtaagt acatgacaaa aattacattt attggtgcag
    10201 gaagtactat ttttgcaaaa aacgtattag gcgatgcgat gttaactcca agtctacaag
    10261 atgcacatat tgcattgtac gatattgatg aaaaacgact aaaagaatca aatttaatgc
    10321 ttaaaaatat taataaaaat tcgaatgaaa atcgtgctga tattgtttct tattatgatc
    10381 gcaaagaagc gttaagagag gctgattttg ttatcaatgc tattcaagtg ggtggttata
    10441 aaccttcaac agtgattgat tttgatattc ctaaaaaata tgggttgcga caaactattg
    10501 gtgacaccat ggggattggt gggattttta gaaccctacg gacactacca gtaatgttag
    10561 actttgctaa agaaatggaa gaagtttgtc caaatgcttg gttattgaac tatacaaatc
    10621 cgatggcgat gttaaccatg ggtgttttaa aagcgacgag tattaagact gttggtttat
    10681 gtcatagcgt tcaagtatgc gtaccagaat tgtttgagca tttaggaata gccgatcaat
    10741 atgacttaaa tgactttcaa tggaaaattg caggcattaa tcatatggca ttcttgttgg
    10801 aaattacaaa aaatggggaa gatttctatc caacaattcg tcagttagct gcagaaaaac
    10861 caaatccaca tccagattct gtacgttttg aattgttgaa acgttttggt tactatatta
    10921 cagaatcaag tgaacacaat gcagaatacc atccatactt tattaaatca aaatacccag
    10981 aattgattga cgaattaggg attccgttgg atgaatactt acgtcgttgt gaacaacaaa
    11041 ttaatgattg ggaaagcatg cgagaggaca ttgtaaataa tggggagtta acacatacga
    11101 gaagccgtga atatgcttca tatatcatgg attctattac gacaggaact ccgtcagtga
    11161 ttgcaggaaa tgtcttaaat aaaggcttaa tcacaaactt accgacagat gtttgtgtgg
    11221 aagtaccttg tttagtcgat aaaaatggcg tgcaaccaac ctatgtcggt gaattgccaa
    11281 cacaattagc tgctttaaat cggacgaata tcaatgttca agaactgaca gtagaagccg
    11341 ctttaacact agataaatca aaaatttatc aagcagcgta tttagatcca catctatcat
    11401 ctgaattgtc tttaacagat atcaaaaatt tagtggatga tttaatcgaa gcacatgggg
    11461 actatttacc tgaatataat taaaaagaag ctgaaagagt aaggaatttt caattcctta
    11521 ctcttttctt agttaaattt agtaaaaata ttgacaaaac acttagtaaa cagttacatt
    11581 agtgttaaca aaaagaaaac gcattcaaat tgtataagga ggagcataaa atgaagtggt
    11641 ctaaaaaaat tattttagct tgtatagcac taggagcaac gatctctttg actgcttgtg
    11701 gaaaaaatca ggaagatgca agcggtaagg tagaaataga gttttttaat caaaaaaaag
    11761 aaatgactca aacgattcag gagattgcta aagattttga agcaaaaaac cctgatattc
    11821 atgtaaaagt tgtggatgta ccgaatgctg gtgaagtaat taaaacacgt atgcttgcag
    11881 gcgatgtacc agatgtcatt aatctttatc ctcaaagtat agaattgaaa gaatgggcga
    11941 aagctggtta tttagaggac ttaaccgaag aaccgtattt agagaatatc aaaaatggat
    12001 atgcacaacg ttttgctatt gaggatcgtg tatatagcat accattaacc gctaatgtct
    12061 atggttttta ctataacaaa actgcttttg aggaaatggg gattcaagca ccagaaactt
    12121 gggcagaatt tgaaaaaata gtagctgaga ttaaagatca aaacaaagta ccgtttgcga
    12181 ttgcaggagc agaaggctgg actttaaatg gttatcacca actagctata gcgactgctg
    12241 ctggtggtga agaggaagcg aacaatgttt ggcgtttctc agaagttaat ggaattaatg
    12301 ctgaaagcaa agaaatgcaa aaagatttta atcgattaga tttgttaaga gaaccaaaag
    12361 ctttgcaaaa caactggcaa ggtgccggtt ataacgatac agtagttact tttacaaaag
    12421 gtgatgcgtt gattatgccg aatggttcat gggcaatgcc gatgattcat tcacaaaatc
    12481 cagattttga agtaggaact tttccttttc cagcagatga agcaggtcaa agtctaacga
    12541 ttggcgcagg agacctagcc ttatcagttt ctgcaacttc tcaacataaa gaagaagcca
    12601 aacgttttgt agaatatatg acaacacctg aagcgatgca gaaatattat gatgtagatg
    12661 gtgcaccgtg tgctgttgag ggcgttatcg agaatactca ggattcacca ttaagtggtt
    12721 taactgaact tgcttttact gatcgtcatt tagtttggtt agccaaagat tggaatagtg
    12781 aaaatgattt ttatacctta acaacaaatt atttacacaa tggcaatcaa aaagccatga
    12841 ttaacgcatt aaatgctttc tttaatccaa tgaaagctga cggaaggtag gaaaataaaa
    12901 tgaaaataaa agaaatttta aatcgttact gggcaatcat ttttgtagca attccaatta
    12961 ttttgcagat gatttttttc tactttccaa tggttcaagg tgctttttac agtttaacta
    13021 attggactgg tctgacttat aattttgatt ttgtgggttt gaacaattat aagattcttt
    13081 tttctgatcc gaagtttatc aaatcgattg gcttcacttt aattgtgacg ctttgcatga
    13141 ttgctggaca aattggtttt ggtattttga ttgcgcgggc gttgaacgca aatattaaag
    13201 ggaaaacatt ctttagagca tggttctttt ttccagcggt cttatcgggc gtggttgttt
    13261 ctttagtatt taaacaaatt tttaactatg gtttaccaaa aattggtagt atgttaggca
    13321 ttgaattttt acaaaccagt cttttaggaa caacatgggg agcgattttt gcagtaattt
    13381 ttgttttact ttggcaagga attgccatgc cagtcatcat tttcttagcg ggttttcaaa
    13441 gtattccaca agaaattatt gaagctgcta gaatggatgg ggcgaatgat aagcaaattt
    13501 tttggaatat tgaaatcacc tacttattac caagtatttc aatggtattt attatggcat
    13561 taaaaggtgg cttaacagca tttgatcaaa tttttgcttt aactggtggt ggaccaagcg
    13621 atgccacaac atctttgggg ttacttgttt ataattatgc ttttaaaaac aatcagtttg
    13681 gttatgcgaa tgctattgct ttagtcttat ttatcttaat tgggattgtt tcactggcac
    13741 aattaaaatt atcgaaaaaa tttgaaatct aggaggtttc ccaatgaaaa catcaaaaga
    13801 aaaaaatatt tctaaatact tgcttttatt attagggggc ttcatgattt tagttccctt
    13861 agtttcaacg ctattcggtt catttaaaac aacacccgat attatgaata atttctttcg
    13921 ttttcctaat ccagcgactt tggaaaattt ccgaagattg ttagcggatg gaattggtca
    13981 ttacttttgg aattcggcaa tcatcacaat cattgcagta gttgctgtga cagcaattat
    14041 tcctatggct gcttattcac tagcaagaaa aatgactcgt gggaaagcat acggattgat
    14101 gtatttattt ttaattttag gaatttttgt tcctttccaa gtaattatga ttccgattac
    14161 cgttatgatg agtaaaattg gtttagcaaa tatgtgggga ttaatcttac tctatctcac
    14221 ttatgccgta ccacagacac tatttttata tgttggtttt attaaactaa atgtgcctat
    14281 cagtctggat gaggctgctg aaattgatgg ggctagtcac tttacaactt atttcaaaat
    14341 tgttttccca atgttaaaac caatgcatgc gacgactatt attcttaatg ctctatggtt
    14401 ttggaatgat ttcatgctac cattattaat tttaaatcgt gattctaata tgtggacact
    14461 accattgttt caatataatt atacgggaca atattttaac gactatggtc caagttttgc
    14521 ttcctataca gtaggaattg tcacgattac actagtttat cttgctttcc aaagaagtat
    14581 tatagctgga atgagcaatg gagcagtgaa ataaccaata aattattctc attataaagg
    14641 agttcataaa cttatgatta tatttgacaa tcacacacaa acatttcatt tacaaaataa
    14701 aaaaattagc tatctattag gaatcgaaga aaataattat ttaacgcatc tttattttgg
    14761 aaaaaagatt acgcactatt ctggtgggta tcgctacccc cgaacagatc gttccttctc
    14821 acccaacaat gcaaatgcgc aagatcgttt attatcttta gacacattac cattagaatt
    14881 tccaggatat ggacatggtg attttagaga accggcgcat aatattaaat taggaaacgg
    14941 tagtcgtgta aatgattttc gttatgatag ctacgaaatt caaaaaggaa agcccagctt
    15001 agcgggattg ccagccttat atacggaaga tgatgatgaa gctgaaacgc tgattattca
    15061 attgaaagac gcgattagtg gattgacttt acagttgttc tacacaattt atcaaaatca
    15121 cagtgtaatt attcgttcta cgcaattagt aaataatagt gatgaaacag tggagattaa
    15181 ccgcttagct agtcaatcaa ttgattttcc aaatcgtcca ctcgagttaa ttcatttaaa
    15241 tggtacctgg gcaagagaac gtcaaatgac tagagaaaaa attcatacag ggataaaagt
    15301 attagatagt aaacgcggat caagcagcca tcaacaaaat ccgtttgttg ctttagtaga
    15361 tgccaaaaca gatgaatttc aaggagaggc atatggattt aatttagttt atagcggtaa
    15421 tcacgaaacg gtgattcaaa aagatccatt tgaccaaaca cgggtcatta tgggaattaa
    15481 ttcatttaat tttggttggc aactaaaaac tggcgactca tttcaaacgc cagaagtcgt
    15541 gatggtttat agtaatgaag gtctgaatac gttatcacag acgtatcatg aattgtataa
    15601 tcaccgttta attcgtggaa actatcgttt aaaagaacgt cccacgttaa tcaacaactg
    15661 ggaagcaacg tattttgatt ttaacgaaga aaaaatcatg aatattgtcg atgatgcgaa
    15721 gaacttgggc attgaaatgt ttgttttaga tgatggttgg tttggcggtc gtgaaaatga
    15781 cttcacttca ttaggtgatt gggttgaaac agaaggcaaa cttgcttgtg gtttggaaaa
    15841 tttagccaag caagtgcatg ataacgggat gaaatttggg atttggtttg aaccggaaat
    15901 gatttctgaa gatagtgaac tatttcgagc acatccagat tgggcattgg gtattccagg
    15961 tcgtgaccgc tcaattagtc gaaatcaata tgttcttgat ttttcaagac aagatgttcg
    16021 tgataatatc tatcaacaaa tgacggcgat ttttgatcgt gtaccgattg attatgtgaa
    16081 gtgggatatg aatcgaaata tgacagaagt ctattctgtc ttgttggact caacgatgca
    16141 aggtgaagtg gctcatcgtt atatgttagg gttatatgaa tttttagaaa agttaacaac
    16201 gaattatcca actattttat ttgaaagttg ttcgggtgga ggcggtcgtt ttgatgcagg
    16261 tttcttgtat tacatgccac aaacttggac gagcgacaat accgatgccg tggctcgtct
    16321 gaaaattcaa tatggaacca gcttagtgta tccaatttca agtatgggtg cgcatgtttc
    16381 agcagtccca aatcatcaaa caggaagaga aacgagttta actattcgtg gcaatgctgc
    16441 gatggctggt gtttttggtt atgagctaga tctaacgcaa ttaacttctg aagaaaaagc
    16501 agaaattaaa gaacaagttg ccttttataa aaagcatcgc caacttttgc aatatggaac
    16561 tttttatcgc ttggaaagtc cgtttgaagg caatgatact gcatggatgt ttgtctcaaa
    16621 agaccaaaca gaagccatgg tcttttattt ccgagtatta gcagaagctg cgcatccatt
    16681 agtcacattg aaattagcag gattagcaga agaagcaact tatcaaatgg aagatgtaac
    16741 atttgctggc gatgaattga tgaatctcgg tttttatatt gactcagaac tacatggtga
    16801 ttatgcgaca caacgctttt atttgaaaca gatatagtag cttgaattta caatataagt
    16861 gcaacaccaa aagagactca ttccaaacga agaaaatatg ttttatttac acgcatttac
    16921 taaaaagcag cctgatttga attgggaaaa tgaagaagta cgtgaagaaa tctatgcaat
    16981 gattaactat tggttagata aaggactagg cggatttaga atcgatgcga tattaaacat
    17041 caaaaaaaaa ctagagtatg gcatctttga atctgatgga gaagatgggt tagccttcat
    17101 tgggccctgg attcttaatc agtcgggcat tgaagtgtgg ttaaaagaaa tgagagagcg
    17161 gacatttaaa ccacataata gtatgacggt agcagaagcc gatgttccta atgaacgttt
    17221 agatgaatat attggtgaag atggctatta ttcgatggtt tttgatttta gttatacgga
    17281 tattgatgta cctgaaactg gtgaatggtt taaagatagc caatggacat ggacggatat
    17341 gcgtaccaat atttttacga atcaattagt tacgcaagat aaaggttggg gcgccttata
    17401 tttagaaaac cacgatcaac cacgttcaat taataaatat attccagaag aatggatcaa
    17461 tgattatagt aaaaaaatgt tggcgacgct ttttatgtta ctaagaggta ccccttttat
    17521 ttatcaagga caagaactag gaatgaccaa catagaaatg gcatcattag aagattttga
    17581 tgatgtagct acgcattctc agtataaacg ggcattagaa tatggattgg caccagagca
    17641 agcattgaaa gctgtcaata aacgaagtcg ggataattct cgcacaccta tgcaatggaa
    17701 tacgcaaaaa aatgccagat tctcaacttc tgataatgta tggttaaaag taaatcctaa
    17761 ttatccagaa ctcaatgcag aagctcagtt aaccaatcaa cagtcggtat tcaattatta
    17821 tcatcaatta attaaattaa gaaaaaatag tgtatacaaa gacgtgctaa tctacggaca
    17881 atttctacca gtgaaagata aaaatgaaca tattttgctt tatgaacgcc aattagaaga
    17941 taaaatcgta ttagttatct taaacgttac agcaacagaa caagaatatt atgtagatga
    18001 acagtaccaa caagtattaa tcaataatta tgaagatgtt ttattagatc aaagtcgaaa
    18061 attgcgttta cgaccgtttg aaagtattgt attagctaat tatggaggac aattatgaaa
    18121 attaaaaacg aagcaatgct catcacctat gcagatagtt taggaaaaaa catgggcgaa
    18181 ttaaaacaag tcttagacaa acatttacaa ggtgttattg gtggtgtaca tctgttgcct
    18241 ttttttccat cgacaggtga tcgaggcttt gcgccgagtg attatacgac agttgatcca
    18301 gctcttggaa catgggaaga tgtggaagca ttaggtgaag attattattt gatgtttgat
    18361 tttatgatta atcacatttc tcgtgaatca aaatttttcc aagatttcaa aaaaaaccat
    18421 gagcagtcac catacaaaga aatgtttatc cgtattcatg aatttttccc agaaaatcga
    18481 ccaacacaag aagatattga tttaatctac aagcgtaaag acaaagcgcc gtttcaaacg
    18541 gtagaatttg cggatggtac gacagaagag gtttggaata cctttgggga agaacaaatt
    18601 gatttagatg tgaccaaaga agttgtgaaa gaatttattc gagaaaccat caaagatatg
    18661 gcgaaccatg gctgttcttt aattcgatta gatgcgtttg cttatgcgat taaaaaatta
    18721 gataccaatg atttcttcgt ggaaccagat atttgggaac ttttagacga agtgcgtcaa
    18781 gaagcagcga aatatgacgt cgaattatta ccagaaatcc atgagcatta ttccattcaa
    18841 atgaaaattg ccaatcatga ttattatatc tatgacttcg ccttgccaat ggtcactctg
    18901 tattcacttt atagtggaaa atcagaacgt ttagccaatt ggttgaaaat gagtccgatg
    18961 aagcagttta caacattgga tacgcatgat ggaattggtg ttgtggacgc tcgtgattta
    19021 ttaacagatg aagaattgga ttacacctct gaggaattgt ataaagtcgg ggcgaatgtg
    19081 aagaaggtct attcaagtgc caattacaat aatttagata tttatcaaat taacagcacc
    19141 tattatagtg cattaggcga taatgaccgt agctatctgt tagcacgtgc gattcaagta
    19201 tttgcgccag ggattccaca aatttattac gttgggttat tagcaggaaa aaatgatatt
    19261 gaactgttgg aagcaacgaa agaagggcgt aatattaatc gtcattacta tgatttagcc
    19321 gaaattgaag aagaagtgca acggccagtg attcaacaat tatttgcact tttaaaattt
    19381 agaaatgaat ctgcggcgtt tgatttggat ggaacgattg aagtagcaac gccatcagaa
    19441 tcagaaatag tgattacacg taaaaataaa aacggtagtg atagtgcaac attaacggct
    19501 aacttggata cgaaagaatt tatcattgag tctaaagata taaaaatttt cactagtgtt
    19561 gcattaaaat tttaacctat tccatagaac aaaaaatcct ttatactagc acttggatga
    19621 cttatccaag accaataaaa aggactcata catggataag tataaaacaa atacagcatt
    19681 taataaatgg ttttctgcca taaaattagg aaaactttct gtcaactccc aaaaatgtat
    19741 ttttgattac aattcgtatc gtaaaaaact gtcttttgaa gctgtgctca agctctttct
    19801 ctacgcagtt aatgaagaaa aagaaagttt gcgtgatttg agtacagctc ttatcgaccg
    19861 atcgctccaa ctagaaaccg atgtgtcatc gattagtcat acccaattgt ctcgagcatt
    19921 taatgcactt gaacctaaag tgttagaaga aatttttcaa caactgttag aaaaagttca
    19981 acatcaaatc aaaccaacca agcgaaatca tctgtatctg attgattcca gtacgttttc
    20041 gcttagttta aaacgtcata agtgggcgac tttccgtcaa acaaaatcag gtgtcaagtt
    20101 acacttaaac tattgttata tggacgatac tacgatgtat ccaactgatt ttaccttaac
    20161 aaatgcccat gaacatgatc tcaatcaact accagtattg gtgaatcaac cagaggcaac
    20221 ttacgtgttt gatcgtgggt attaggactt tgaaaagatg gatcagatgc attgggatgg
    20281 ctatttcttc gtcactcgga ttaaaaaaaa caccaaagtt cacgtgatag atactctgga
    20341 aacatcgcca gaaacagaaa ttctacgaga cgagctggtt cgtttaggat ctaaaaccta
    20401 tctaaccgcc aatttccgac tagtcactgt tcaagataag aacggacgtg tgtttcaatt
    20461 tatcaccaat cgaatggacg tctcttcaaa agaaatctct gatatgtatc acgctcgttg
    20521 gcaaatcgaa ttgtttttca agcacatcaa acaacacatg accattaaaa ccttcttttc
    20581 tcaaagtgaa aaaggcgtgc agaatcaatt gattttaacg atgatttccg ctctcctcac
    20641 gtttctcatc aaattggaga cgaaaacgga aaaatcagtg ttccaaatca aacgattctt
    20701 tcgttattta ctgtttcagc cctttgaatg ttgtctagaa aaattaattc ccacatgaga
    20761 aaaaagaaat ttggctcgtt acttttggtt ttcagttgaa taacagataa aaaattttct
    20821 ggaaaaagtc atcctctgcg tcagcacagt tttttttgac ttattttcag aggaaatagt
    20881 tttcagaata ttctaaaatg ttttatgcaa cactagtgat attttttaga attagatttc
    20941 taatgtgcat tttttgagta ttaaggagca agtatacaaa gatttataaa gctgattaaa
    21001 tttgaataaa aatatgcagg aacgctacta tttttagtag tgttcttttt tatatttgaa
    21061 taaaaaatgt ggtggttaaa tggtggttat aacaaaaaat gaattaatga aattaggtta
    21121 tgggaagtat cagtctgaga gaatcatacg acaggcaaaa gcaattatgg tttcaaaaag
    21181 ctatacctat tacgaaaata gaagattagg actagtacct gttgaatctg tagaagaagt
    21241 tttaggtatg aagatagagg tagaggagga ttttcagaat catggttaat tttaaaaaag
    21301 ataaaaatgg gaaatatcat tttactgcta gtttgggatt cgacaaacga acagggaaaa
    21361 gagtacaaaa aaacgtagag gggttaaaac agttaaagaa gaaaaaaagc gtataccgaa
    21421 cttgtttcta attatggaaa agaagcagaa acttctcaaa gtactatgtt gtttgaggag
    21481 tttttttata aattattcct accttattat caagggaaag tagaagaaag aacatataaa
    21541 aatcggttat ctacaatcaa aaaaactttt cttatttctt taaaatggag ttaagagaaa
    21601 tacgtccaat tgacattcaa aagtggcaaa ataatttatt gagcaaatat aaaaattctt
    21661 atgtaagaaa catgtatggg atttttcaga tgtcattaga tcgagcagtt gttttgggac
    21721 tacttgaaaa aaatcctgca aaagttattg gaaatgtgaa gaaaaggaaa actacagttg
    21781 atttttggac aaaagaagaa tttacaaaag ttatttcaac tttttatatt gatgatttgt
    21841 accaacattt tggttttatt tgtatgtggt tactttttat gaccgttatg cgtttgggag
    21901 aagccactgc attaacttgg aaagatattg attttgaaaa taaaaacttg cgtgtgaata
    21961 aaacactcta ttataagaga gccaaaacct atgagtttgt agaacccgaa acggcttcta
    22021 gtattcgtac aatcgaacta gacgacaata caattacctt tttaaaagct tggaaggagc
    22081 gtcaagtttc aataggaggc atgtcgtttg tactttctta taatggaaca cctactcaga
    22141 aatatacaat ccgtcatatt atgagaagac atgcaacgat cgttggtgtt catataatcc
    22201 gcatacacgg attaaggcat agtcatgctt ctttgcttat atcacaataa aaagatgcac
    22261 ttcgaattaa agaaaggtta aaacatgctg atgtacaaac gactttagga acttatggtc
    22321 atttgtatct aaattcattg agagagataa caaatgatcc cacagattat atccaaacta
    22381 atttttctaa aatcaaaccg acacctattt acgataataa aaaataaatg ataaacaaaa
    22441 gtaggatcac accaaaagaa acacactaga aaaactataa tacaggcttt tctagcgtgt
    22501 tgtttcttat ttccgcctat ttatacatta tactcataaa gcatttaatg catcgatcgt
    22561 agccgcattg tctatatggg tatataaagt tgttgtattg gtacttgaat gtcccaattg
    22621 ttgggcagtt aataccaatg aatttgtctg ttggtagagt ctagtagcta atgtatgacg
    22681 cagtttgtga gggctgactt gaattctaaa gccttttgaa tattttgcta ccatccgttc
    22741 gattgcaata ccggatatac gcttggcttc tccttggtaa attgctagaa ataaagcttt
    22801 ttcgttttct gttactttat atcgttcttt ccgaatagct agataattag ctaaatattc
    22861 cattgcgaat gctgctatat tgacagcatc tcttttattt ccttttctag ttactaccac
    22921 attattctta tctaaagata ggtcatctaa attaatattt ataacctcgg agagtctcaa
    22981 tccagatcct aaaatcagtg cattgatcgc caggtctctt tctacatctc gttgataata
    23041 gatcaaagct cgtttactca aagtattttt atattcattg gcaatataat ctagataatt
    23101 ttgtgtatcc ttatctagaa ataatttttt ctcaatggca ttggcacgtg cagctaacgt
    23161 agttttatct gtgatggttg ctacttgatc cataggatta ttgtctaagt atggttttcc
    23221 tagaggatta ttggaacttt tcgaaagata tttaaataga acctttaatg ccgtgatatt
    23281 tctattgata gtagaagtag atagacctaa gttattatta tctttgagct tagtttttgt
    23341 tgtctctttt tttacatttt cttttgtgcg acgtttcaaa tgagatttgt acaagtttag
    23401 gtcataggca gttaactgtc ttatcgaggt gatagaagta tcaggaacga tagactcact
    23461 cagaagccat tcataaaaga gcttataatt ttttagatat tctaaagtgg ttcgaggtga
    23521 ttgatcttta tcctcacgat cttgaacaaa atcttttata taatcgggca tatgaagtat
    23581 gattgtatct attttttgat atatttctgt atttttcatg tcattcatcc tttcaataag
    23641 taggggagaa gagtatctct ataattggac tataccaatt atagaatgtt ttgtcaattg
    23701 gtatgctcta aaatttatta atttgtttca aaaaaagcat ataaacaggt atacgtttaa
    23761 taatttaaaa taaatggtat acataaatag ggaaggaaat agaagggagc aatctaaatt
    23821 ttttgaattt tcttaattaa ttatatagaa agaacaatct ttctatagag ttagacatga
    23881 gagggactat gcttgattta aaatcaacaa gcgtatatac tgtatataca aagaaaaagg
    23941 agggagaaga ctatggcaat taaagaaaga aaatttaggg aagtggggaa ctcagtagta
    24001 ttaactattt caaaggagtt tcttaaagaa aatgggttaa atcccggaga tacggttttt
    24061 ttagatgaag aaaaacttcg agcggcaatt acaaaaaaac aagccttatc tgatgaaatt
    24121 gatttagcaa tatctcaagc attaactgaa tatgatacta cttttagaga gcttgtcgat
    24181 aaatgagtat tcattaccta gaagccaaag atattataaa aatgaatgta atccaaataa
    24241 aaaaatactc tcctaatgaa ccccttggtg taaaggatgc gaatgcttta gaaatgtgtg
    24301 tagggcaact tcgagcctca gcttttggtg aagaggttta tccttcgatt tatgaaaaag
    24361 cagctatcct acttatacaa ttgataaaga aacatccttt tcacaatgca aataaacgta
    24421 ctgctttctt agctactttt gtctttttga aaatcaatgg acagctttta gctattgatc
    24481 aaaaagatgc tgtaaattta gcggtatata ttgctacgta tgataaggat ttcgatcaac
    24541 taaaatatga agtgatcaac actataaaga cacatacgca gcccatttaa ggagttactg
    24601 tatgtgtctt tatagggtta ttagctgcta tagcgaaata attgaagtaa attacaaata
    24661 gctataattc aaatccaact atatagaaaa cgtaaatttt ctatatagtt ggattaagat
    24721 aacgactatt tattttatca tatttttact ataccctata cttttacttg gtatatttat
    24781 gaataatcgc aaatttaccc ctatactact agtttgtaag ttagattaat aaaaatacta
    24841 aagttatttg ttcttttatt aaatacacct acaacttata aattgatggt atttagataa
    24901 cacgaaagaa taaaaataac agctgatagt aaagtttgtt gacgattgct actcaatcga
    24961 aagtgagtta gtgcgtatga acttgcttct gaaaacttat gaaatgaaag accttttaca
    25021 tcttgcagaa atcgttactg ggattaaact aagcaataaa aactaatgtt cccctcattc
    25081 tttgaacttc tactatttga tttatttatt ctgataaatg acgatttcac cttcaggatc
    25141 ttcaaatccc gtatagaatt tatttaagat ctcttcagta tcagtaatag gataggcgat
    25201 aggccacata ccctttgatg gttagaataa actgctcaaa atctgtatcg tatgttggct
    25261 ttagcatatt aacaaaggct atctccaaat tataaaccat aagcgttgaa tcgttcttaa
    25321 caatttcaat tcttcctaac ccaaagtcag aagatataat taccggactt gccttaccct
    25381 tcatgtgggt tgttgagatt ccatatttaa agggaattgt cggtactatc ggcggtctga
    25441 aaaacatccc aaaaataaaa aaatctttcc cctgctagtc tcctaagtcc ctttgtagcg
    25501 gatatggcac cataatctca ttttatcgaa gtgagtcatg gtttatctgt tggccaacca
    25561 aaattgatac aatctaacga tgcaccctct acggcattct aggggagtgc acttgcattc
    25621 acccaataaa aaccggcagt tagcaaagaa cttatgtagt cgtatgttct tactgttaac
    25681 atacaagagg tctcctttcc acaattgctt agcagtaaac gggaatttac aaagctaatc
    25741 tcttccattt tcttatcttt gttctaaaag tcccaaatgg agcaactgta tttacatgaa
    25801 taaatttata tacttcccat acagccgttt tggttgcctc gtctgcccat tttcttttgt
    25861 gtgcagtgaa tagatcttca tcactcattg aatcaatcat catgttaata tcatcaatat
    25921 tacgattgag aatttcctct aattctttca atggtagatg tgcataggta tttgtaaacc
    25981 acttatataa ctctcctaac tgattccatt taaattgatc agaaggcgtt ttaacagata
    26041 atccatttct ttcatcattt tcccatttta gaattaaatt cgtccaacca acttgatatg
    26101 ctaaattttc agcaggtgtt ctatccactt cctcaactct tttatccttc aattcttcag
    26161 gaatagtttc aaattctaaa atatatttag tataagtcct atttatttct ttttttaatt
    26221 cttctttatt tttatatgtt ctcattatat tacctccata aattcctaat tgtctccttt
    26281 aattcatcta taatttcatc cacgatattt taatccgttc tacaatgcat atctgatatg
    26341 cattgactgt ctaactcata tgtgaatgat ttataaaata tcccaacgtc tcatctaaag
    26401 aaatgttgat cgtcagcaag gagctaaaaa atacgaatat atttcttttg tagtaaaatc
    26461 ggactggttt gcatatcatt ttcttcgtaa atatcaaaat catgttaact actatcaatt
    26521 actaataaaa actaccactt taagcgttaa gaactgttaa gtttagttaa gtgaggatag
    26581 ttcacaaaat ccattgttaa acttcacgaa agtggtaaaa ctcaaaatgc tttatatagg
    26641 attacagcat cgctctttct tcgattgcgc gttgggtaaa aaatattatg aagtaaaaat
    26701 tgacgatgac acgttcctta cggctcatca gatgaaacag ctacaaaaac gaaatgatca
    26761 actcgaagaa aatttaattc taaaaaagcg attgccatat tcacgcctcg ctcgacgaac
    26821 tattaaaggt caagatttac ctgaagatca tcctttttag gaaattgaag ctttttatgt
    26881 tgcacaatgc atttataata ctacaatgat gttttcacca gatattcttc tagggtgcgg
    26941 tgtaatgaaa cagatacact tgcaaaagaa aattcaccgc gaatttacta aaattatgaa
    27001 taattatgtt aatcttccga aatttttttc caaaattaag ggatcaagct ggtgttatag
    27061 attgtttaag gttggcaaaa aaataacttc tttttaaaca attatagaaa accgatactc
    27121 tgttaagttg gttgacacgg tgtcgatttt tatgactttc ttattctgaa acttcactaa
    27181 cggacagtac ctaaaaagtc tatagtatgg acgtttacga gtactatttt atcaaaaatg
    27241 ctgctcattt caacttatat gttttagtga actaccccca cttagcttgg ataggctaca
    27301 attaactaga cagaaaaatt aaggtgtgta gactagaaga aaacatacca ggaggaattt
    27361 ttatgtctaa gagaacacga agaacttttt cacaagaatt caagcaacaa atcgtcaatc
    27421 tttacttagc tggaaagcca cgtgtagaaa tcattcgaga atatgaacta acggcttcag
    27481 catttgacaa atgggtaaag caatctaaaa cgagt
//
LOCUS       DAZKOB010000025        26089 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000025 DAZKOB010000000
VERSION     DAZKOB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.025"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..731
                     /locus_tag="ACG8M6_001091"
     CDS             <1..731
                     /locus_tag="ACG8M6_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0456772.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="ACG8M6_001092"
     CDS             789..2828
                     /locus_tag="ACG8M6_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0456773.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2937..4088)
                     /locus_tag="ACG8M6_001093"
     CDS             complement(2937..4088)
                     /locus_tag="ACG8M6_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0456774.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4242..4772)
                     /locus_tag="ACG8M6_001094"
     CDS             complement(4242..4772)
                     /locus_tag="ACG8M6_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0456775.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4871..5221)
                     /locus_tag="ACG8M6_001095"
     CDS             complement(4871..5221)
                     /locus_tag="ACG8M6_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0456776.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="ACG8M6_001096"
     CDS             complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="ACG8M6_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0456777.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="ACG8M6_001097"
     CDS             complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="ACG8M6_001097"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0456778.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7624..8286)
                     /locus_tag="ACG8M6_001098"
     CDS             complement(7624..8286)
                     /locus_tag="ACG8M6_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0456779.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8470..10116
                     /locus_tag="ACG8M6_001099"
     CDS             8470..10116
                     /locus_tag="ACG8M6_001099"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0456780.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10107..11189
                     /gene="uxuA"
                     /locus_tag="ACG8M6_001100"
     CDS             10107..11189
                     /gene="uxuA"
                     /locus_tag="ACG8M6_001100"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0456781.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11297..12850)
                     /locus_tag="ACG8M6_001101"
     CDS             complement(11297..12850)
                     /locus_tag="ACG8M6_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0456782.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12998..14167
                     /locus_tag="ACG8M6_001102"
     CDS             12998..14167
                     /locus_tag="ACG8M6_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0456783.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(14293..15372)
                     /locus_tag="ACG8M6_001103"
     CDS             complement(14293..15372)
                     /locus_tag="ACG8M6_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0456784.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15481..15921)
                     /locus_tag="ACG8M6_001104"
     CDS             complement(15481..15921)
                     /locus_tag="ACG8M6_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0456785.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(15978..16388)
                     /locus_tag="ACG8M6_001105"
     CDS             complement(15978..16388)
                     /locus_tag="ACG8M6_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0456786.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16569..17003)
                     /locus_tag="ACG8M6_001106"
     CDS             complement(16569..17003)
                     /locus_tag="ACG8M6_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0456787.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17103..17951)
                     /locus_tag="ACG8M6_001107"
     CDS             complement(17103..17951)
                     /locus_tag="ACG8M6_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0456788.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18340..20106
                     /locus_tag="ACG8M6_001108"
     CDS             18340..20106
                     /locus_tag="ACG8M6_001108"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0456789.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20208..21467
                     /locus_tag="ACG8M6_001109"
     CDS             20208..21467
                     /locus_tag="ACG8M6_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0456790.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21614..22915
                     /locus_tag="ACG8M6_001110"
     CDS             21614..22915
                     /locus_tag="ACG8M6_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0456791.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            22918..23769
                     /locus_tag="ACG8M6_001111"
     CDS             22918..23769
                     /locus_tag="ACG8M6_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0456792.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23782..24597
                     /locus_tag="ACG8M6_001112"
     CDS             23782..24597
                     /locus_tag="ACG8M6_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0456793.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24748..25914
                     /locus_tag="ACG8M6_001113"
     CDS             24748..25914
                     /locus_tag="ACG8M6_001113"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0456794.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         8822 a         4730 c         4844 g         7693 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     2941 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3001 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3061 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3121 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3181 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3241 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3301 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3361 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3421 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3481 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3541 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3601 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3661 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3721 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3781 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3841 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     3901 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     3961 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4021 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4081 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4141 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
     4201 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
     4261 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
     4321 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
     4381 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
     4441 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
     4501 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
     4561 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
     4621 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
     4681 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
     4741 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
     4801 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
     4861 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
     4921 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
     4981 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
     5041 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
     5101 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
     5161 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
     5221 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
     5281 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
     5341 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
     5401 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
     5461 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
     5521 tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga
     5581 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
     5641 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
     5701 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
     5761 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
     5821 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
     5881 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
     5941 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
     6001 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
     6061 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
     6121 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
     6181 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
     6241 ctgcactact agctgctggt ccaccgccaa ttactaatac atcataaggt tctttttcaa
     6301 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
     6361 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
     6421 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
     6481 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
     6541 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
     6601 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
     6661 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
     6721 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
     6781 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
     6841 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
     6901 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
     6961 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
     7021 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
     7081 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
     7141 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
     7201 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
     7261 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
     7321 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
     7381 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
     7441 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
     7501 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
     7561 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
     7621 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
     7681 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
     7741 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
     7801 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
     7861 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
     7921 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
     7981 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
     8041 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
     8101 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
     8161 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
     8221 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
     8281 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
     8341 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
     8401 catagcgatt tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg
     8461 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
     8521 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
     8581 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
     8641 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
     8701 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
     8761 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
     8821 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
     8881 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
     8941 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
     9001 attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
     9061 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
     9121 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
     9181 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
     9241 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
     9301 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
     9361 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
     9421 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
     9481 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
     9541 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
     9601 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
     9661 ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
     9721 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
     9781 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
     9841 ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc
     9901 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
     9961 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    10021 gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac
    10081 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    10141 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    10201 cattgatgga tataccagta ggagaggtat ggcccaggga gaggatcaaa gaactaaaag
    10261 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    10321 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    10381 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    10441 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    10501 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    10561 aatttgaatt gccgggatgg gaacctgagc gcttagctca tatcaaatcc ttgcttgaag
    10621 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    10681 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    10741 actcgatttt tggattacct cgaatcgtaa agaatcgaga agatttagat tggttgtgcc
    10801 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    10861 cagaaaatga tgtatatggg attttagcag aattttgtca aagagatcga atcccatttg
    10921 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccgcataaat
    10981 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    11041 acgaatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    11101 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    11161 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    11221 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    11281 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    11341 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    11401 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    11461 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    11521 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    11581 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    11641 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    11701 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaaca
    11761 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    11821 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    11881 caggtataaa caaatcaaat acagtacaaa aggactccac catttctctt gaataaagaa
    11941 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    12001 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    12061 ttggcagatt aggtcgcaaa atatctacag agggatcaat aaaaaagtca atatattcat
    12121 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    12181 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    12241 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    12301 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    12361 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtacaatg
    12421 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    12481 gctgacttaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    12541 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    12601 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    12661 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    12721 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    12781 gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt
    12841 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    12901 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    12961 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    13021 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    13081 gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    13141 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    13201 catcaaagca aaaattgact attcaggaga cttagcacct atactacaat cagctgttac
    13261 atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca
    13321 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    13381 gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta
    13441 tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga
    13501 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
    13561 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
    13621 agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc
    13681 agaagaaaaa ccggatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc
    13741 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaactag aggtagatcc
    13801 agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc
    13861 agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa
    13921 tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag
    13981 cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgcta gtcctgaaac
    14041 gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac tgtggagcgg
    14101 aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac aaaacagatc
    14161 tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa atatccagaa
    14221 aacgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac tttatctttc
    14281 ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga tctttttcac
    14341 tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc cccaaacgcc
    14401 aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca gttctgtttt
    14461 gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa tcaaaatcgt
    14521 aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga aagatagagg
    14581 aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc
    14641 taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga gcaacgtgtt
    14701 taaaagagcg ataacaaatt gagcttctaa taccaaacca aatgtattga caaacttatc
    14761 ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa acaatcttga
    14821 gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga atgacatagc
    14881 aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg aagcgccatt
    14941 ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt gtaaaacagg
    15001 atctgcatct tttggctgat gctcgtaaaa atcaataacg gactggatca tatcaaatgt
    15061 ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt aaacgatcag
    15121 tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct gaatgatttg
    15181 aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg taatcatact
    15241 tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc gtattttttc
    15301 attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac aaaaaataaa
    15361 cacgttattc attatatgat ggaatatgaa aataagaaag agaaagagca aaaagaaaaa
    15421 ccgctaaatg acgttatctt atgagttggt tttcctcata aaatcgttca tctagcagtt
    15481 tcagcccttt ttctggtctt gatgaaaatt atctagcatt tttcgggtca gatgttgcag
    15541 tgtatccaat tcttcctgtt caagtggaag ataagacttg atcttttgtg gaataccttc
    15601 tgcttgtttt tgcaacgcag caccttgagg agtcagcgta acatttacga ttctttcatc
    15661 acccgtttca cgctgacgat taatataatt tttttctgct aactttttta gtaagggagt
    15721 cagcgtacca gaatccagac acaaatcatt tcctagctct ttcactgaag tctttttctt
    15781 ttcccataaa actaacatca caagatactg ggtataggta agattcagcg gcttgaggag
    15841 ggaagtgtag tgtctaatga tttccttaga ggcggcatat aaaggaaaac atacttgatt
    15901 aactaaatat agatcttcca tttttccacc tccgctgttt tactatagtg tatcatagcg
    15961 gatttacaca aaaaagtcta ttcactaact gctttcagag taacttctat atttccagct
    16021 gtagcttttg aatacggaca cactttatgc gctgcctcta ataattcttg cgtttcttcc
    16081 agagaaagac cttcgataga tccttcaatc tctacaccta agacaaagct cgtttcactt
    16141 tctgaataaa gggaaacttg tgcactgaca gtagaagccc cagagatatt tttttgctgt
    16201 ttgatcaatt ctaatgcact gttaaaacat gcactgaaac cagctgcaaa caattgttca
    16261 gggttggttg cattttcgac gcgtttacca ggagctgcaa tattcaattc gaatgtacga
    16321 tcgggattat gaacttctcc agagcgtccg cctgtgttga tcattttcgt agaatacatt
    16381 tttttcataa aattaccacc tttaattata ttttatacaa ttgaatttta cacaatataa
    16441 ttgtggaaag caaagaaatg gattggcata agataaataa gagaaagcac atgaaacaga
    16501 cgcgctttct cgaatagcaa acatatacaa aaaaatatca gagacaaaaa ctaatctcat
    16561 cataaacatt acatatattt ctcaaaaagt tgttttcttt cttcaaaact tacttcacga
    16621 tggacctgat gaagcatcca taaataacca aaaggatctc tgaacattga gttagtcaca
    16681 ccaaagtcca tcatttctgt catttcttga atctctacac agccatttcg taatgcttta
    16741 ttatgtgtgt cctcgatatc agagacgatg atattgaacc agatcgtatt tggatgttca
    16801 gcagtaggag cagtcaaacc aagagacgtg ttttcatcca gcatatggaa tctcgtgcca
    16861 tacaaggtaa aaatgacttc attattgcct ctagggtaat ctgttgcctc aatcagttgt
    16921 atttcatcaa agattgtttg atacagttct agtgctttca aactatcatt taatacaaaa
    16981 tctatttccg ttcctttatg cataaagatc tcctctcctt ttcagtcgaa cagttactag
    17041 attcttttat tataccagtt aaagaaaacg cattcaaaaa aatagcggat ttacttacca
    17101 actcattcga ttggatgata agtaaatccg cgtcccatta cttctgctac gctgctgaca
    17161 atgacaaagg cacttggatc gatttcttct atcaatggtt tcattcggga aaattcatga
    17221 tcagtcacaa cgatcaataa aacctctttt gactttcgat tatacccgcc ttttgcctct
    17281 agcagggtaa ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc ttccaaataa
    17341 tgttcactca tgaccataac agatttcttt cgattgatac ctgtttcgat ataagtcatg
    17401 acaattgaag tgattgcaat agataaaata gcataaagaa actcttctat tccaaaaaca
    17461 aacagattaa gagaaacaac gaatgcgtct gtaaaaaata agccaatcga agtattcaag
    17521 tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga agagttgttt
    17581 ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac agacaacaac
    17641 cgatctgaag taatcattgt ttctggaact attgcaatga ttaaaggaaa aaacaagctt
    17701 ccgaaaagta ctttaaaaaa gggtttttta cctaaaaata gaagagctag taccaacata
    17761 acgatattca acactaaaat aactatagct cggtcaaatc ctaaaacagc ttccagtaat
    17821 attcctagac cgctgacacc tccagcagca atatgatgag gccctaaaaa catattgata
    17881 gcgactccta atatgaagag ggcaaaaata atcttaatgt atgtccatat aaattgtctc
    17941 gtttctttca tttatccact tccttaattc attaataata ctcttatctt attataactt
    18001 acttttcacc ttaagttaac tttaattaat aaaaaaatta ggctagtcta aattattatg
    18061 tttcattctt ctagaatata cttctacgtt aaaaactgtt aaaaaacttt tactttagaa
    18121 aattggagaa attgatcgct taagataaat tcaggacact aaatagtaca ggaagtagat
    18181 taaaactttt aaaagtttta agtctatcta tttggcaagt ttaaaaaata aaaattcggt
    18241 atcaaaaaac tgttaccgat aacatttttg aaagcgttgt cttttgtttc tctatcactt
    18301 atagtgatga cgtcatatta aaacacaagg aggaaatcaa tggatacagc tgcaatttat
    18361 catcgaccgg aaagtgaata tgcatttcta tatacgaata caagatttcg tattcggctt
    18421 cgtactagga aaaatgatat caaaaaagta tatgtgcttt gtggagatcc atataccata
    18481 acaactgaaa aatggtatca aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt
    18541 catgattatt gggaaattga agtgacgagc gaaacaaggc gcttacagta tgcgttccat
    18601 gttgttgggg aagataacac ggactgtttt tacggtgatc aaggaatttt tccttatcga
    18661 gaaaatgtga ttactgaacc aaatttttat tttcgaatac cttattttca tcaaatcgat
    18721 cgattcacta ttccagaatg ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga
    18781 tttgcaaacg gggacaaagc aaatgacccc gaacacactt taccttgggg aagtaaagat
    18841 ccagaccgag aagacttctt cgggggagat ttgcaaggtg tgagggatca tttggattac
    18901 ttgacggacc ttggcgtcaa tggcatctat ttttgtccca tatttaaagc cacatccaat
    18961 cataaatacg atacgattga ttattatgag gtggatcccg cttttggcga taaaaaactt
    19021 ttaaaaaact tgattgatga agcacataaa agagggatac gtatcatgtt cgatgctgta
    19081 ttcaatcata tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa
    19141 tcgatctata aagactggtt ccacatccgt tttttcccag tagattctta tcagatgact
    19201 gacttaccag aaactgctga aaatatccca tatgatactt ttgcttttac tccattcatg
    19261 ccgaaactaa atacagcaaa tccagaagtg caaaaatatt tgctggatat cgcaacttat
    19321 tggataaaag aattcgatat tgatggatgg cgtcttgatg ttgctaatga agtcgatcat
    19381 catttttgga aaaaattcag agaagcagtt acagaaatca aaccagacat ctatatcctt
    19441 ggagagattt ggcattcctc tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg
    19501 aattatgcat tcacagattc gattaaagat tattttgcaa aaaagaaaat cacagcttca
    19561 caaatggtca gtggaatgaa tcatcagcaa atgctttaca gagatcaggt gaacgaagga
    19621 acattcaact tattagattc tcatgatact gccaggatct tgaccctttg ccaaggtaac
    19681 aaagaattga tgaagtctgt gatggccttt atgtttcttc aaaaagggtc tccttgtatc
    19741 tattacggga cagaaatagg gatgactggt gaagatgacc cggattgccg caaatgtatg
    19801 atatgggaga aggaggaaca agatctagag ctattcggtt ttataaaaga acttgtttct
    19861 ctaaggaaac agttgtctaa gattatttct gaaggttcta ctcagtggct aatagtatat
    19921 gatcgtgaag ataagctcta tttcacacgg gaactagagg gacagatcat ctatgtttac
    19981 tttaatcagt caaaagaacc ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa
    20041 aattgtcagt tgttagaaga cgggaaagca gagatacgac aatacggatt tttgatcatg
    20101 tgttgaaaac tgttaccggt aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat
    20161 tataataagg gtgtaacagc acgttgaata cacaggagga aatgaagatg aaaaaaagaa
    20221 gtgtgaaaca gttgggttta gggttaatct ctttaggaat cttaggcgga ttagcggctt
    20281 gtggcaatgg taactcttca gcagaagggg aagccgatga aggaaaaacg ttaacagttt
    20341 ccgttgattc tggatataaa gattatataa acgaaataaa aaacactttc gaaaaagaaa
    20401 acgatgtgaa aatcaagcta gtagaaaagg acatgttcga tcagttggaa tcattagcgt
    20461 tagatgggcc tgctggaaag ggacctgacg ttatgatggc agcatatgac cggatcggtg
    20521 cgttaggaca gcaaggacat ctggcggaag taaaactggg aaatgaagca gcttacgatg
    20581 aaacagacaa agcccaagtg acttatgatg gaaaaatcta cggagaacct gcagtaatcg
    20641 agacccttgt tttgtactat aacaaagatt tagtcgatac agcaccatca acgtttaaag
    20701 atttggagaa tctatccaaa gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata
    20761 caggattttt agctaaatgg actgactttt attattccta tggtttaatt gcaggttacg
    20821 gtggctatgt atttggtgat gatggcacaa atccttcaga tatcggtttg aataatgcag
    20881 gtgctgtaga aggaatctct tatgcaacag attggttcaa aaatgtttgg ccaaagggaa
    20941 tgcaagacat aaaaagtgca ggagacttcg ccagccagca attcatgtcc aataaaactg
    21001 cagcaatcat tgacggtcca tggcaagcgc aaacatacaa agaaaacaat atcaattatg
    21061 gtgtagccaa aattcctact ctaaataatg gacagcctta tcaaccgttt ggcggaggaa
    21121 aaggatgggt agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt
    21181 atgtaacgaa tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa
    21241 accaacaagc tagggaaaca gcaaaggcta aaaacgatga attaacaaca gctgtgattg
    21301 aacaatatga aacagctcaa gcgatgccaa acatcccaga aatgggcgaa gtttggacag
    21361 gtgcagaaaa cttgatgttc gatgcagcat caggttcaaa aacaccaaaa gaatccgcag
    21421 atgaagcagt aaaaacaatc agtgaagcca tagaacaaaa atactgatta aaatgataac
    21481 cgtccatttt tataaaaaat ggaacaggtg cttcagataa aagtggcaag agcgagatgg
    21541 aaaattatct tgctcttgtt gccattatct taaaagcagt ttgcggttta gcaagtttag
    21601 agggggcatt cagatgcagc aagataaaac aagcaatatc aacgaaaaaa aaatcacgct
    21661 ttgttcactt attccaggga tggggcagtt tgtaaatggc caagctttta aaggtatcgt
    21721 ttttttcgcc atattcgtcc tattcattgg tcagatgctt ctgggcggat ttcatgcatt
    21781 ggtaggattg gttactttgg gaagtatacc aatggaagat cattcattat ttattttgat
    21841 tgaaggcact ttgcaattca tcattacaat cattttcttg attttttacg gtttgaatct
    21901 tttagacgct tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca atcggacgat
    21961 gaaggaagtc atacataata taggtgatga aggttttcct tatctattga cacttcccgc
    22021 ttatcttctc atgattttta cgatcatttt cccagtccta gttacattgt tcactgcatt
    22081 tactaattat gacttcaatc atattccacc agccagttta attgattggg taggggctaa
    22141 aaactttttt agtatcttct ttttaagctc atatcgtaat actttttttg cagtcttttc
    22201 gtggaccgtg atttggactt tatgtgcaac aactttgcaa atcacccttg gtattctcac
    22261 agcggttgtt gctcaccaga aatttattaa attcaaacgt attttcggta tcattttctt
    22321 attaccatgg gcggtcccag cttttattac tatcatgagt ttttcgaata tattcaacga
    22381 tagcgtaggt gccatcaata ctcaagtgat tcctttcata aaccagcttc cttttgtaga
    22441 tatcagtgca atctcttgga aaacggaccc gaactggacg aaagtagcaa ttatcttagt
    22501 gcagggctgg ttaggatttc cctatatcta tgtgatggtt acaggaatct tacaatcgat
    22561 ttcagaagac ttatatgaag cagcaaaaat cgacggagca aatgcatggc agcgcttttc
    22621 aaacatcacg cttcctgctg tcttcctagt ggcagcgcca acatttatta cacagtatac
    22681 ggggaatttc aataatttct ccatgatcta tctgtttaac aatggtggtc ccggaagcgt
    22741 aggcggagga gctggttcta ctgatattct tatttcctgg atctataaac ttacgactgg
    22801 aacttctccg cagtattcta tggcttcagc aatcacgttg attatctccg tcatcgttat
    22861 ctgtgtgtca ttgctggtat ttaaaaagac aaatgcgttc aatatggggg attaacaatg
    22921 aaaaaatttc attcgagtgc tcgattccaa aaacgacttt ctcacttttt tacttacttt
    22981 tttcttttaa tactgtcagt cattatcatc tatccgcttt tgatcacagc aagtactgcc
    23041 ttcaagtcag gaaatattgc agcttttagt ttagatcttt caagtgactg gactttagga
    23101 aactttactc ggctattcag tgaaacactt tacgggactt ggtataaaaa tacattgatc
    23161 atttctgtcc taacaatgat cgtacaagtg actgtagtaa ctttggcggg ctatacttat
    23221 agtcgctatc gttttatcgg ccgcaaatca agtttgatgt tctttttagt gattcagatg
    23281 gtaccgacta tggcagcatt gactgccttt tatgtcatgg ctttcttatt agaagcgctc
    23341 gataagtact ggttcttgac aatgatctat ataggcggtg gaattccaat gaatacttgg
    23401 ctgatgaaag gatactttga cactgtacca atagatttag atgaatctgc taaacttgat
    23461 ggggcagggc atttcaggat ttttttccag attgtattac cgcttgtcaa accgatgctc
    23521 gccgttcaag cattatgggc ttttatggga ccttttgggg atttcatgtt agctaagttc
    23581 ctgcttcgaa cacctgaaaa tcagactgtt gctgtcggct tacaaacatt tatttctgat
    23641 gtgacaaacc aaagagtctc tctttttgct gctggggcaa ttttgattgc actaccgatt
    23701 tgtctattat tcttcttttt acaaaagaac tttgttaccg gcctgacatc tggcggaaca
    23761 aaaggatagg agcagagtat catgcaaaca tttccgttaa actatttttc cgcattacgt
    23821 acgccaactc aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt tttcttgata
    23881 tttgtatttt tagtttcctt gatggtcatg cctgtgactc ttttttatgc caatcagatc
    23941 caagcgattc cattagaaca atttctatcc gttcattcgc tgatcgatga acaaggaacc
    24001 caaaaatttt ctgaacttga attatctgaa actgggttac aagcatcgca acaaacgata
    24061 gctgtgaccc ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca atccgatcat
    24121 ggaactttta ttgattttga gaaggaacaa tgggtgatcc aacaaaagga taaatctggt
    24181 atacgccgct atacgatgaa ttatagccca tctttccaac cagattctgt acgtacgccc
    24241 gaagatttcc aacgattttt agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt
    24301 ttatcttata gtttaagtct tggtcttgtc ctatttgtca tgacgagtct cattttattc
    24361 ggggcttcct ttttcttgtg gatgacaaga aaaagccaac tttcatccat tcatacattc
    24421 aaagagagtg caaatttgat gctgaatgtt atgggaatag gttccatggc agcagcagtt
    24481 gtagggttta tccactttga ctttatcttg atgctaagtg tccaaacaac gataactgtc
    24541 ttgctgcttt tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa
    24601 gtttaagcga tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa
    24661 aaatttctga aaaaattaaa cttcttagtt gattcttttc ttttttagta ttaacgtaat
    24721 agggtagttt ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa tcaattattt
    24781 tcacgttctt tctcgctccc tgtcgaagta aattattggg acggtacaac gaaacaatac
    24841 ggagatacgg acactcctcc ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa
    24901 gagattacaa gtaacgcttc tttggcacta ggagaagcct atatggatca tcgaatcgag
    24961 atcgagggaa gtatccaagc attgatcaac gatgcttatc agcaagcaaa cagcttttta
    25021 cgaggaaaag attatctaaa atggctgcca aagaaagaaa aacatacgaa acaaaaaaac
    25081 aaagaagata tccatgcgca ttatgactta ggtaacgact tttataaact ttggctagat
    25141 ccaactttga cttattcttg tgcttatttc aatacacctg aagatacgtt ggaacaagca
    25201 caaatcaata aagtccatca tatcttagac aaactcttta tcaaagaagg cgaaacgttg
    25261 cttgatatcg gttgtggctg gggaacattg atgttcactg ctaccaaaga gtataatgtc
    25321 aaggcaactg ggatcacact aagtgaagaa caatacgatt tgattaccaa aagaataaaa
    25381 gaagaacatt tagaagataa atgccgtgta ttattaatgg attaccgcga actaaaaggc
    25441 gaaacgtttg atcatatcac aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt
    25501 gaaggttatt tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc tttgatccat
    25561 ggaatcagcc gacaacaagg cggtgcgaca aatgcttgga tcaataaata tattttccct
    25621 ggcggttatg tccctggtgt tactgaatta gttgataata ttacaaaaaa cgatttgcaa
    25681 ctgattgatt tggaaagctt acgtcgtgac taccaattga cattagaaca ttggacaaag
    25741 aattttcatc aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg
    25801 tgggatctat atttacaagc ttgtgcagct tcatttgaat caagtaatat cgatgtgatt
    25861 caatatttgc ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc ataattgagg
    25921 caaaagtact gtttaaagac tatttaaaaa gaagctaagt tttatgatgc tccagaagtg
    25981 aacttttttg tctaacttct aggattgtct ctaaagctta gtttcttttt ctttatattt
    26041 tcataataaa agtttggttc tttaattttt aaacatgaca gaaaatttt
//
LOCUS       DAZKOB010000026        25566 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000026 DAZKOB010000000
VERSION     DAZKOB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.026"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8M6_001114"
     CDS             133..3660
                     /locus_tag="ACG8M6_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0456795.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8M6_001115"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8M6_001115"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0456796.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8M6_001116"
     CDS             7448..7789
                     /locus_tag="ACG8M6_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0456797.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8M6_001117"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8M6_001117"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0456798.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8M6_001118"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8M6_001118"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0456799.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8M6_001119"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8M6_001119"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0456800.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8M6_001120"
     CDS             13000..13737
                     /locus_tag="ACG8M6_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456801.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8M6_001121"
     CDS             13751..14566
                     /locus_tag="ACG8M6_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0456802.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8M6_001122"
     CDS             14579..15226
                     /locus_tag="ACG8M6_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0456803.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8M6_001123"
     CDS             15242..15898
                     /locus_tag="ACG8M6_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0456804.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8M6_001124"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8M6_001124"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0456805.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8M6_001125"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8M6_001125"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0456806.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8M6_001126"
     CDS             18240..18758
                     /locus_tag="ACG8M6_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0456807.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8M6_001127"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8M6_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0456808.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACG8M6_001128"
     CDS             complement(19645..20565)
                     /locus_tag="ACG8M6_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0456809.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACG8M6_001129"
     CDS             20691..21743
                     /locus_tag="ACG8M6_001129"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0456810.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8M6_001130"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG8M6_001130"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0456811.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG8M6_001131"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG8M6_001131"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0456812.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACG8M6_001132"
     CDS             complement(23383..24144)
                     /locus_tag="ACG8M6_001132"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0456813.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ACG8M6_001133"
     CDS             24333..25427
                     /locus_tag="ACG8M6_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0456814.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8472 a         4432 c         5392 g         7270 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgat
//
LOCUS       DAZKOB010000027        25514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000027 DAZKOB010000000
VERSION     DAZKOB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.027"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG8M6_001134"
     CDS             complement(104..1336)
                     /locus_tag="ACG8M6_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0456815.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG8M6_001135"
     CDS             1591..2160
                     /locus_tag="ACG8M6_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0456816.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG8M6_001136"
     CDS             complement(2338..2778)
                     /locus_tag="ACG8M6_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0456817.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG8M6_001137"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG8M6_001137"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0456818.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG8M6_001138"
     CDS             3732..4655
                     /locus_tag="ACG8M6_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0456819.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACG8M6_001139"
     CDS             4731..5870
                     /locus_tag="ACG8M6_001139"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0456820.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACG8M6_001140"
     CDS             5863..6663
                     /locus_tag="ACG8M6_001140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0456821.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACG8M6_001141"
     CDS             6663..7490
                     /locus_tag="ACG8M6_001141"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0456822.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACG8M6_001142"
     CDS             7495..8202
                     /locus_tag="ACG8M6_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0456823.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8M6_001143"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG8M6_001143"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0456824.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8M6_001144"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG8M6_001144"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0456825.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8M6_001145"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG8M6_001145"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0456826.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8M6_001146"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG8M6_001146"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0456827.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACG8M6_001147"
     CDS             11787..13820
                     /locus_tag="ACG8M6_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456828.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACG8M6_001148"
     CDS             13864..15144
                     /locus_tag="ACG8M6_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0456829.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACG8M6_001149"
     CDS             15354..16160
                     /locus_tag="ACG8M6_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456830.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACG8M6_001150"
     CDS             16172..17392
                     /locus_tag="ACG8M6_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456831.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACG8M6_001151"
     CDS             17382..18968
                     /locus_tag="ACG8M6_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0456832.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACG8M6_001152"
     CDS             19007..21145
                     /locus_tag="ACG8M6_001152"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0456833.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACG8M6_001153"
     CDS             complement(21513..22496)
                     /locus_tag="ACG8M6_001153"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0456834.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACG8M6_001154"
     CDS             22874..24265
                     /locus_tag="ACG8M6_001154"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0456835.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="ACG8M6_001155"
     CDS             24281..25321
                     /locus_tag="ACG8M6_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0456836.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..>25514
                     /locus_tag="ACG8M6_001156"
     CDS             25340..>25514
                     /locus_tag="ACG8M6_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0456837.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGR"
BASE COUNT         8095 a         4314 c         5212 g         7893 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgta
//
LOCUS       DAZKOB010000028        25479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000028 DAZKOB010000000
VERSION     DAZKOB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.028"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..2151
                     /locus_tag="ACG8M6_001157"
     CDS             91..2151
                     /locus_tag="ACG8M6_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0456838.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2234..2803
                     /locus_tag="ACG8M6_001158"
     CDS             2234..2803
                     /locus_tag="ACG8M6_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0456839.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2978..3784)
                     /locus_tag="ACG8M6_001159"
     CDS             complement(2978..3784)
                     /locus_tag="ACG8M6_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456840.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3922..5436
                     /locus_tag="ACG8M6_001160"
     CDS             3922..5436
                     /locus_tag="ACG8M6_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0456841.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5839..6822)
                     /locus_tag="ACG8M6_001161"
     CDS             complement(5839..6822)
                     /locus_tag="ACG8M6_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0456842.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6827..7474)
                     /locus_tag="ACG8M6_001162"
     CDS             complement(6827..7474)
                     /locus_tag="ACG8M6_001162"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0456843.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7559..8191)
                     /locus_tag="ACG8M6_001163"
     CDS             complement(7559..8191)
                     /locus_tag="ACG8M6_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0456844.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8207..8758)
                     /gene="coaC"
                     /locus_tag="ACG8M6_001164"
     CDS             complement(8207..8758)
                     /gene="coaC"
                     /locus_tag="ACG8M6_001164"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0456845.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8755..9492)
                     /locus_tag="ACG8M6_001165"
     CDS             complement(8755..9492)
                     /locus_tag="ACG8M6_001165"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0456846.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9489..10478)
                     /locus_tag="ACG8M6_001166"
     CDS             complement(9489..10478)
                     /locus_tag="ACG8M6_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0456847.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10736..11278
                     /locus_tag="ACG8M6_001167"
     CDS             10736..11278
                     /locus_tag="ACG8M6_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0456848.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11295..12380
                     /locus_tag="ACG8M6_001168"
     CDS             11295..12380
                     /locus_tag="ACG8M6_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456849.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12392..13198
                     /locus_tag="ACG8M6_001169"
     CDS             12392..13198
                     /locus_tag="ACG8M6_001169"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456850.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13199..14026
                     /locus_tag="ACG8M6_001170"
     CDS             13199..14026
                     /locus_tag="ACG8M6_001170"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456851.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14023..15096
                     /locus_tag="ACG8M6_001171"
     CDS             14023..15096
                     /locus_tag="ACG8M6_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0456852.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15524..18220
                     /locus_tag="ACG8M6_001172"
     CDS             15524..18220
                     /locus_tag="ACG8M6_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0456853.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18285..18785
                     /locus_tag="ACG8M6_001173"
     CDS             18285..18785
                     /locus_tag="ACG8M6_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0456854.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18797..19291
                     /locus_tag="ACG8M6_001174"
     CDS             18797..19291
                     /locus_tag="ACG8M6_001174"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0456855.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19312..20121
                     /locus_tag="ACG8M6_001175"
     CDS             19312..20121
                     /locus_tag="ACG8M6_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0456856.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20118..20939
                     /locus_tag="ACG8M6_001176"
     CDS             20118..20939
                     /locus_tag="ACG8M6_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0456857.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            20990..23089
                     /locus_tag="ACG8M6_001177"
     CDS             20990..23089
                     /locus_tag="ACG8M6_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0456858.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23086..24204
                     /locus_tag="ACG8M6_001178"
     CDS             23086..24204
                     /locus_tag="ACG8M6_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0456859.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24243..25121
                     /locus_tag="ACG8M6_001179"
     CDS             24243..25121
                     /locus_tag="ACG8M6_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0456860.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25267..25464
                     /locus_tag="ACG8M6_001180"
     CDS             25267..25464
                     /locus_tag="ACG8M6_001180"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0456861.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8036 a         4440 c         5405 g         7598 t
ORIGIN      
        1 catcttcttt tgattttatt tatataatga aatcatctta tgaaaattac tacttcatgt
       61 ttagacaaga gtagggagga ctaagaataa atgtgttcaa aaagacgttc aaaacaacta
      121 aataaacacg cttgtcgttt agataaggaa cggtcgtgtt ctgaaaaaca ctacgttaag
      181 tttatgatgc gcacgatgct gatcagttta tctgtaggtg tctatatgca tgtacccgtt
      241 caggcaacga cacaaaccat caaggtcgta cgtacctctg tttctgagac cactgattcc
      301 cctatcacta ttactcatac tttcactgat gcaaatggaa aacagctact tcctgcattg
      361 acttcctata ataaagtcag ttatcctcca acgataaacc actactggtt aattcctaac
      421 aaaacattat ggacatcgga aaccggaagc agtgtgccgc tttctatctt ttccgatatg
      481 actgatccta caaaaggtga tcagttaaaa cagatcactg atattttgaa tcatgctttg
      541 acagatataa aggacggtgg aagcgcacaa gtttcttatg tatatgaaaa agattattca
      601 cagtttgaaa gaaagaaaat cgatgttctg gcagggacaa aagtttcgat atctgatctg
      661 gtagaaaggc tgaagaacgc agatggaaca caaggagata tgtccaaagt aatttcaaac
      721 aatgggaata tcgtggatac gactaataca ggaacatcta ctatttcctt gtcctatttt
      781 gatccaattg ctttaaaaac aatgactgca tctgctgtga tccatgttcg tgctgatcaa
      841 acagctatca aaggaaaaga tcttcactta agaacaggag agagttacgc ccctaaagat
      901 cttgttgaat atgtaacaaa aagcgatgga acttcaggaa ataaagaaga aatcaaaatg
      961 aacgggacag tagatactac aactccggga gaatatcccg ttgtattaac ttatatagac
     1021 tcgaaaacat caaatgtggc agaaacaaca gctgtagttt atgtagaagc atctgctgct
     1081 tcatcgactg tcatcactca ttttatagat agtcaaacaa ataaagaact tcaagcagaa
     1141 acacatatga gtagtgtaaa tcgtattcct gatttgcgcc tacctgctga attttctgat
     1201 cgagtgccaa atatagattt atcgacttat gaacaaaatg ggaaagtcac ttctatttct
     1261 gattggatgc aagcaaacgg attagacaga aaaagtcact ggtcggagat ccttgaagta
     1321 cttcactcag aactgatgaa taataggaag caatcaggag gggaaattac ctttacttat
     1381 gtttatatac cagacaatcg ttcattagaa ggtcatgatc ttattgttca tccaaacgaa
     1441 aaaataaaca aagaagagtt gatccaatca gcaacaaatt ccttaggtaa acccgtaccg
     1501 ttaaaggatg tatcggttac aatcaatgaa gtaccattgg aggatggtta tttgaccaaa
     1561 caaacaggtg attttacgat tatgtatagt tatcatgacc cgtattcact ccaagaactc
     1621 catgctcagt cactattaca tgttttacca ttaaacaaac agatagatca aaaagaacaa
     1681 aaaagacaag aagaaagctt gtcagaaaaa aaacaacaaa tggctcatgc agtttctcca
     1741 acagggaaag taaaacaaac aagaagccta gagttagcaa gaatatctag aaaaatctca
     1801 gaatctaata aaacgataag gcaagaagct catttggccc cacaagtttc tggattggaa
     1861 aatgaaattt tagagcctaa aataaaacaa caatcagaaa aagaatttgt tgctaaacag
     1921 tcggaagatc agaaggaatc agatgaccag aaggaaacga agaaaaaacg cttgtcaact
     1981 attgattcca ccgatgagca tctgcagccg caagtaccac ttcctagtcc gggaacaccc
     2041 gcacctggtg gaggtattcc ttcttctgct ttaggagatt tcatgcgagg tatggcagga
     2101 acatgggtat acgctaatcg aaatgaattt gacgcagatg actggttctg atttatatct
     2161 ctatctgtta tagtcaaata agtagctgag tcatatgtta tttggcataa tggagacgag
     2221 gagggataga aaaatgttag ataaaacgtt accttacgca gaaatctgga tgacaagacc
     2281 acttcaacaa aatgttccaa tcacgccttt agcggaaggt tttgaatttc gattttatca
     2341 aaaagaagat gaatctgctt gggcggagat tgagacttcc gttggggaat ttcaaacgac
     2401 agatgaagca cttgagtatt ttcaacaaaa ctttgctcct tatcctgaag aacttgaaag
     2461 aaggatgttt tttgtagaga caaaaaaagg agaaaaagta gcaacctgta ccgcttggcg
     2521 aaaaaaatca agagagcaag tgtatccggt ttttcattgg ttagcagtga aaccagatta
     2581 ccaaggaaaa gggctcgcaa aagcattgac tgccagagtc ttgcaagaat ttcctaccct
     2641 ccatacggac ggaccaatct atcttcacac acagacatgg agccatcagg cgattgctct
     2701 ttataaaaaa atgggtttta cttttattgc tgaaaatcta gacggcacac ctaatccaga
     2761 ttatgaaaaa gttatgcagg ttttaagtaa attagataaa taacgaaatg gtgaaataag
     2821 tcctcagtat tgggataaaa aacgaaatag tcattcctca tatcttactt ttttgagtta
     2881 atactatcgg ataactaatg gcatgccata cattcaaaaa agaaggggca gtgacaaaag
     2941 tattgtcact gccccttctc ttgtttttta gcatgtttta tcgtttgatt tttttaggaa
     3001 taaaattctt gatgaacgta aatttttggt aatcgtttcg actatagagg taaccaaata
     3061 cactgaaaac aactgctcca ataaaattaa caaataaatc cttcatagta tccatgattc
     3121 ccacgtctaa gtaaccattt tggatgattg ttttatctgt tttaccatct gcattttgac
     3181 tttcaatgat tgtccgagtg atatctggaa catgatagac agtgtttccg tctttactta
     3241 actccacact attgatttgc tggatgatcg tatctttttg catatcgaat tgcagtaatt
     3301 tatctgctga aaattccaca aactcccaca taacaccaac tgtcatcgaa aaacagaagg
     3361 aaacaactgc tagtaataaa ggagtcaagt tcatggtttt tgtattttta ttgagcaaat
     3421 aaacaaggga aaagccgaca cccccagcta aaaatccgct gaatgcatgt agaaaagtat
     3481 cccacattga aaaatagcca taaaaatcac ttaactctcc taaaactgtt gaactaaaaa
     3541 taaaagataa caaaatgatt tctagcatat ttggaatctg tatctttaat atcttttcca
     3601 gaaaataagg gatttcaaat aaaattaatg tcagtaataa aagcattgta tttccccagt
     3661 gttggttcca gacctgatcc actagcataa taacgacgat gatacgtaat aaaatataga
     3721 ttccgtttgt gattctacct gcagtttttg tactgttcgt aaaaaaggtt ttcaaatgat
     3781 tcataaaata actccttcat gtatatcctt aattatacta agaagcgtgt gtttaccaaa
     3841 gctatatgac ttaaagaaaa acagagttga tattgtgaaa aaaatcactt aatatagtat
     3901 aaggagaaaa gggggataca aatgaaaaaa ttagttgctg gtttattaac agccgttgca
     3961 cttcttgcta tgagcgcatg cggcgcagat cagaaaaata cagcgcagac aagttctagt
     4021 acgaaggaat ctgccgaagt atcatcaggc gcttctgaac aagtatatac agatccaaaa
     4081 gaaatgaaag aatcttatga tgtcgtgatt attggagctg gtggtgcagg gatggcagca
     4141 gctatcgaag caaaagatgc tgggttgaat ccagtaattt tggaaaagat gcctgttgcc
     4201 ggaggaaaca catctaaatc atcaagcggt atgaatgctt cagaaacaaa atttcaaaaa
     4261 gaagaaggaa tcaaagactc gaatgatgcc ttctatgaag aaacgctaaa aggtggtgga
     4321 ggaacgaatg ataaagaaat gcttcgatac tttgtcgatc actctgctga tgcggtcgat
     4381 tggctagatc aaaacggtat caaactggac aacttaacga tcactggagg gatgagtgaa
     4441 aagaggacac atcgtccgtc tgatggctcg gctattggtg gatacttagt ggaggggtta
     4501 ttgaataacg tccatgagcg ggaaatccct atttttgtca acgcagatgt gacagacatt
     4561 aagaaaaatg gggaaacggt caatgaagtg actgtacatg ttcaaggaga agaaccaaaa
     4621 gaggtcaccg gaaaagcaat cgtagttacc acaggcggtt ttggtgccag caaagaattt
     4681 attgaaaaat atcgtcccga tttagctgat tatattacga caaatcaaga aggatcaact
     4741 ggagatggta tcaccatgat tgaaaaatta ggtggacaaa ccgttgatat ggataaaata
     4801 caaattcatc caactgtgca gcaggatgag ggggtcttga ttggtgaggt tgttcgcgga
     4861 gaaggagcaa tcttggtcga tcaagagggg aaaagatttg tcaatgaaat ggatacaagg
     4921 gataaagtat ctgcagcaat tacagcattg cctgaaaaat cagcttattt gatttttgac
     4981 caaggcgtgc gtgatcgtgc cacagctatt gaattttatg accaaaaagg atatgtcaca
     5041 actggaaaga caatggaaga attagcagaa aaaatcgatt taccaaaaga aacactagca
     5101 aaaacagttg atggatggaa tacggatgtt gcggagaaaa aagatgctca attcaaccgt
     5161 gccacagcca tggaacatga cttatcgtct ccgaattatt atgcgatcaa aatcgcacca
     5221 ggaatccatt acacgatggg cggtgtgaaa atcaatacga acactgaagt attagataaa
     5281 gaaaaccagc caattagggg gttatatgct gctggtgaag tcacaggtgg cttacatgga
     5341 aacaaccgga tcggtggaaa ttcagttgcg gagatcatta tttttgggcg acaagcgggc
     5401 atacaagcag ctcgatttgc ttcggaaaat gaatgaaata gaacgtaaat aaaaagaggt
     5461 tgtgaaatca gcgttttgag ctaataccga gggtcagctg attgaacact gtttagtgaa
     5521 gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc
     5581 ttatgttttt aacttggttg gacttattac cgcagatgca agcttttgaa caccgtttat
     5641 tcggaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
     5701 taaacggcgg aagcagaaga aattccttcg gaaataagct gaaattcaca aaaatttgaa
     5761 aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
     5821 tttttgatca gcatgatttt attcagttat cttcaaaatg atttttccga tgtttttgtt
     5881 tgcttccata tattcatgcg cttgtttgac tgcgctgaat tggaatgtac ggtcaatgat
     5941 tggccgaatc ttgccatttt ctagaaaagg cccagctttt tgcataaaag tttgtgcgag
     6001 ctctgcttta tactgatcac ttctaggtgt caataatgta ccaagcagtt ggacacgttt
     6061 cccaatcaat acaccaaggt ctatctcagg cacgatggtc ccacctagca taccaatcaa
     6121 gacccaacgt ccatcaacgg ctatagaagc taaattcttt tcccagtaag aagcgccgat
     6181 gaaatccaaa ataacatcga ctccccgacc ttcagtgatt tttcgtacct cttcttgaaa
     6241 gtcttgtttt ttatagttga tcaattcatc tgcaccaagt tctttacaaa aggcgagttt
     6301 ttcttctgat cctgcagtaa cgatgatttt cgcgtgagaa agctgtctag ctaattggat
     6361 cgctgctgtt cccacaccac ttgctcctgc atggatcaag acagtttcgc cttcttgtaa
     6421 ttttccaagc cagtaaagtg tttgataagc agtcaaaaaa acttctgaga ttccagcagc
     6481 agaaacatag tctagcgtat cagggagaag gattgcacga tcggcaggca tcacagcata
     6541 ttcggcataa ccacctagat tgactaatcc gtatactttt gtcccaggag taaaagtgga
     6601 gatatcactt ttattctcaa ctacctctcc agctacttcg actcccaaaa tagggtaggg
     6661 aggtttcaaa gagactgatt ctcttttcat aatatctgtt cgattgacag ctgctgtatg
     6721 gactttgatc agtaattcgc cttgttttgg tttaggaaca ggcacttcaa tttgttctaa
     6781 tacatctaca gaaccaggat gcttgattga aatggcacgc atggatctat tcctcctttg
     6841 cccattcttg gaatgctgta ttcaactctt tgctaccttc agaaagtact agagtcgtac
     6901 gagaaatatc cgtttcttcc actagagcgg caagtacagc agcaactgta gagcgagtga
     6961 tcgtttttga tgccttctca ttaggcttag ttaattgaat ggaagtaact tcttcatcgt
     7021 tagtcaatgt gactggttga agaatgacaa aatctaagtt agttcgtttc agccattcat
     7081 ctgcataatg tttagtaata taataatctg tcatggactc aggccatttt gctggttcat
     7141 ctgcataaac agcactgacc atcagatagc gagagacatg cgcattttca gctgcgatca
     7201 ctgttttgat tgcgccatcc aagtctactt gcagaagatt ctttccttga gagccggcag
     7261 caaaaatcaa tacatcgatg cctttgaatg cttcggtcat tttttccacg gaccaagtta
     7321 aatcaaaaga gacatacgaa acattctctt tttttactaa tgtttgggaa gcaacatctc
     7381 tcacccctgc aaaaatttca tgggaacttg gagctaaatc ttttatcagg tgtcggccaa
     7441 tttgtccgtt tgcacctaca acaaaaattt tcatttggat catcctttcg acgtcaaatc
     7501 tttccattca atagtaacac caaaagagaa aaaaatcaga gatcggctac gatgactatc
     7561 agttagtaag agaagggtgc ttgaagtatc ttaacaagac attgcatact gctgcagtcg
     7621 caagacctgc gacgattcct tcaggtatac cacttgtaaa aataaccgct agaatagctg
     7681 aataaacagc atttaaattt gttccgatga ctttggcata atcttgctgg aataaaaagt
     7741 agatcatatt catcactaaa atcgtattgg tcaatgaacc ggaaagtcca gctaagaata
     7801 aagaaacgga atctgctttt tgtttcatta gtttgcgcag acctttataa acgaaataag
     7861 gaaccacccc aatcaagatg cgaggaataa acgcgatcag caaggctttc cagcttccat
     7921 gttctgttcc gatgactggg ataaacggag aaaagacaaa agacaaaggt gtttggataa
     7981 ctgtgctacg gatcatgctg atcagtccaa atgtaccgcc aagaaaagca cctagtcttg
     8041 gtcctaaaac gatcgaagca atgattacag gaatatgcat ggttgttgca ttgattggac
     8101 cgatagggat aaaacctaaa ggcgtaacag ctaataaaat caaaatagct aaaaacatcg
     8161 cagttaatgt aaagtttttt gtatttttca taagtactcc ttttttctaa gcatttcttt
     8221 cattaagagt cagcatgacc tgttcgatga tcgtctgaat atcagctaag gcaccttttc
     8281 caaaatctcc acaagctaaa agagattctc ttggttcaat ttcctgatag ccgacagatc
     8341 tcaatgtttc cagattacgc tgagtgatcg ggttttgata catattcgtg ttcatggcag
     8401 gggcaatcaa ttttggtgtt cccactggaa gcgccattgc gactgttgaa agaagatcgt
     8461 ctgcaatgcc gtttgctagc ttgccgataa tgtttgctgt agcaggcgcg atgagaaaca
     8521 agtcagcttg ttttgctagt tcgatatgat tgattacctg cggatttgat tcttgcatga
     8581 cgtctgtatg gactgcgcgc ttcgacagag attgcaaggt taatggcgtg ataaaatgtg
     8641 tgctgctttt agacataatc acatctacct gggcacctaa tttgacgaat tgacttgtaa
     8701 gatcagcgct tttgtaagca gaaatacttc cagtaacgcc tagtaagatc tttttcattt
     8761 tgattcctcc agtctaattt tctcagtaat caatgcagca atctcagatt tactttgagc
     8821 ttcttccact gttccatctt tagataagag atagccgtgg tgtgtctcgc catggatttc
     8881 cgtcaaatca tttgcgaaaa taaaatcagt tttgttattt atcagactat tttttgcgac
     8941 tgccagaaga gattcctttg gtacatctac taatagtttg aaaccaatga tttttgcttt
     9001 tggctgccat tcacgtagat aagaaatgat ttttggtgtt ttcttcaaaa cgagcacaag
     9061 atgctcggtg ttagaagaga tctttgaaac agtggtatct gtctgttcat tttccgcaaa
     9121 ccaattgtcc agttcttctt gcgttgaaga agtgggtagg ttttttgcca gctgttcttc
     9181 tgaaaaacta aaagcaggag taaaatcact gactgccata ctgtggataa tcgcatcgta
     9241 gtgttctttt ttcgttagtt cttctaattg taaaaataag tccagtgcag attcgatcgt
     9301 ataaatcgtt atattggagt gtcttttcgg tttcagtgct tgtttcgtag tcacgtaatc
     9361 gattgtggta tccggattgg cagctaaata atcagctaat gcttgcccta atcgtcctgt
     9421 cgaatgattt gtaatagcac gtacttgatc gattttttca gtcgttcctc cagctgtgat
     9481 tagaatcttc atttatggtc acctacattt actagtgttc ttccttggtg tgttccagca
     9541 agtagtttag atgctgtttc aggcaattga tccagagtaa tctcttggac gaccagttca
     9601 tctgcaatct gcaaatccgc catctgttgc cataaagaaa gacgttgatc tattggtaca
     9661 ttgacagaat caattccaat caactggatg tttcgcagaa taaaaggcaa gacagtcgcg
     9721 tttagtttga ttcccccagc attgccacat aaagctactg caccgttata agagatcaat
     9781 ggaagaagac tgcttagctg ctcgccacct actgtgtcga tgacatagtc gatttgctgt
     9841 tttcctaaag cttttgttgt ctctggcaga aattcatcag gtcgtacgac ttgcaaagca
     9901 cctagacttt ttagccatgt gacagactct tcttttctgg aaaaggtaat gattgactgg
     9961 aatcctagtt tatgtaacag ggctatcgct gtactgccta caccgccaga agcaccagta
    10021 acgacaactt ttttatcgga ggtcatacct tgacggatta aagcgttcac acacaacata
    10081 gcagtaaacc ctgctgtacc aaggatcatc gcttgacgca gcgtcatatt ttttggtaga
    10141 ggaactaacc agtctcctgg tactttttga tattggctat aaccgcctgg atgggtgacg
    10201 ccaagcccaa aaccagtagc aagtacaaga tcttcttttg aaaaacgtgg attgttactt
    10261 tggacgacag ttccggataa atcaatccct ggagtcatcg gatatgttcg tatcacctga
    10321 ccagactcgc tcattgccaa tgcatcttta taattgacat cagaataggc aatttttacc
    10381 aagacttcat tttcagccaa ctcagtaaat ggcagatcac tgattgctgt atggaaaaca
    10441 ggttccttta ttacctgaaa agatcgaaaa gcgttcattc gtgagacact ccttttaaaa
    10501 ttttctttta tcataccata aagcctactc ttttattctt gttcttccgt aaatttagac
    10561 aaaaattagg tgaaaaataa agaatcaaac aactagaatt tattccaaaa gataaagaaa
    10621 tattcagcaa aaaaaatttt acaaagggtt gcctttttca ataaatttcg tatactaata
    10681 cgagtggtag taaacaggaa gtttactatt agaaaacttc gattcggagg aaattatgga
    10741 aattggtgag aagttaagaa atttgcgcat ccaaaagaat ttgacacaag aagaactagg
    10801 cgaacgcaca gatttgtcaa aagggtatat ttcccagttg gaacgagatt taagttcacc
    10861 atcaatggaa acattctttt ctattcttga agtcttaggt gtgactccag aagaattttt
    10921 tcatcaagaa gcagcgaatc tgcaagtggt ttattcaaaa gaagatcata cggtttattt
    10981 cgatgaagaa aacgggtatg aattggaatg gctcgtggct gattcgaatg aaaaagaaat
    11041 ggagcctgtc ttgctgactt ttgaaggtgc aggagaatat aagacgtttg aaccttcatt
    11101 atctgaaacg tttatttttg tattagaagg ggaattagag ttaagcttag gagagacggt
    11161 ctatgtggca aaagcaggac agtcgatcta ttatcaagcg acaaaacaac atcaattaag
    11221 gaatcatctc aaaaaacgaa caaaagtatt gattgttgca acgcaatcat atttgtaata
    11281 tagggggtaa aaacgtgggg aaaccaatca tttcatttga tcaagtagtg aaacgctatg
    11341 atgatgaaac agtattaaaa aaagtaagtt ttgagatcga acaaggaaaa ttttatacac
    11401 tgcttggtcc ttccggctgt ggtaaaacaa cgatcctacg tattatagct ggattcaccg
    11461 aagctacaga aggggacatc tattttgaag ggaaacggtt gaatgatttg ccagctaata
    11521 agcgacaagt aaatacagtc ttccaagatt atgcactttt tcctcatatg aatgtttttg
    11581 ataacgttgc ttttggattg aagatcaaga aaatgtccaa atcagatatc gaaaagaagg
    11641 taaaagatgc tttgcgtatg gtccagcttc ctggatacga aaaacgggag attagtgaaa
    11701 tgtctggtgg tcaaagacag cgtgtagcga ttgcacgagc aattgtaaat gaaccgaaag
    11761 tattgctatt agatgaaccg ttatcggcgc ttgatttgaa actacgtaca gatatgcagt
    11821 atgaactgcg ggatctgcaa caacgattag gaatcacgtt tatttttgtt actcatgacc
    11881 aagaagaagc gttggcaatg agtgatgaga tttttgtcat gaataaaggg aaaatcgtcc
    11941 aaagtggtac acccgtagat atttatgatg agccaatcaa tcatttcgtt gctgattttg
    12001 ttggggagag caatatcgtg gacggtgtaa tgatcgaaga taatctagtt gagtttgttg
    12061 gtaagcaatt tgaatgtgtc gatggcggga tgcgtctaaa tgaaccagtt gaagttgttt
    12121 tacgcccaga ggatttgacg atcacgacac cagataaagg aaaactagtc gtaacggtcg
    12181 atactcagct gtttcgtggt gtccattatg aaatcatctg ttatgatgaa cagcaaaatg
    12241 aatggatggt ccattcaacg aaaaaagcta aagaaggaag caaagtagga ttggcctttg
    12301 agccagaaga tatccatgtc atgcgtttca acgagtctga agaagaattc gatgcacgtc
    12361 ttgacagcta cgaagaatag gggggacaag aatgaaaaca atgcgcagga tctattccgt
    12421 cccttatatt ttgtggctcg gtttattcgt gatcgcgcct gtattaatga tcatttatca
    12481 atcttttttt gatatgaatg ggcaattcac gctgaataat tatctatctt attttacgtc
    12541 ggggacatcg ctttctatga cgatcaattc tgtatggtat gcatttttga tcacgttttt
    12601 cacgctgatc atcagttacc cgactgctta ttttttaaca aaactgaagc acaagcaatt
    12661 atggctgatg ctgattattt tgcctacatg ggtgaatctg ctgcttaaag cttacgcatt
    12721 tattgggatt ttcagtgtgc acggcagcgt gaatcatttc tttgatttta tcgggatcgg
    12781 gccgaaacag atattattca cagacttcag ttttctgttc gtggcaacgt atatcgagat
    12841 tccatttatg attatgccaa ttttcaatgc attggaagag atgaatcctt ctttgatcag
    12901 tgctagtaga gatttaggcg cgaatagctt tgagaccttc cgacgagtga tttttccgtt
    12961 atcattaaat ggtgtgaaaa gtggtgtcca agcagtattt attccttctc tttctttatt
    13021 catgctgact cgtctgattg gcggaaatcg ggtgatcact ttaggaacgg ctatcgaaca
    13081 gcatttcttg gttacacaaa actgggggat gggttctaca atcggtgtga ttttaatcat
    13141 tgctatgttc atcgtgatgc tcctgacagg cgagaagaaa aaaggaggtc gctcataaat
    13201 gaaaaaattt aaatggtcgt atctttattt agggatcgtc tttttattct tatatgcacc
    13261 gatcttctat ctgatttttt attccttcaa tgaaggagga acgatgaaca gctttacagg
    13321 atttacttgg gaaaattatg aagctgtttt tgaagataca cgtttgatca ttatcgtatt
    13381 gaatacgttc atgctggctt tcttatctgc attgatcgcc acgatcatcg ggacatttgg
    13441 ggctatggga atctattaca cgaaaaaacg acgtgcacgg acagcgttgt tgagcatgaa
    13501 taacatctta ttagtctctc ctgatgttat tatcggtgct agtttcttga ttttctttac
    13561 actactagga ttcggattag gttttcagag tgtattgctt tcccatattg ctttcagcat
    13621 tccaatcgtc gtcttgatgg tcttgccaaa attgcaggaa atgaacgatt caatggtcga
    13681 tgcggctaga gaccttggtg ctaataattt ccaagtagtc aaaaatatca ttttgcctta
    13741 tctttctcca ggaatcatag ctggatattt tatggcattt acctattctc ttgatgattt
    13801 tgcggttaca tttttcgtca caggaaatgg ctttacgacg ctctcagtag agatctattc
    13861 tcgtgcaaga caaggaatca gtttagaaat caatgcgcta agtgcgttag ttttcttgtt
    13921 ctccatgata ttggtcattg gatactactt tattagtaaa gaaaaccgtc caaaacggct
    13981 gaagaaaaac agaagagtac gcgagggggt ggcacggctg cgatgaaaaa attacaatct
    14041 ctgtttttag gtattctcat cattattgcg gtgctgctgc ttggtgttcg tcaacttgaa
    14101 aaaacaagtg gcatgagtgg agcaaaggta cttaccatct ataactgggg agattatatc
    14161 gatcctagtt tgatcacgaa atttgaaaaa gaatatggat acaaagtgaa ttatgaaaca
    14221 tttgattcta atgaagcgat gttcacaaaa atccagcaag gtgggacaaa ctacgacatc
    14281 gctataccaa gtgaatatat gatccaaaaa atgatcaaag aaaaactagt acttccatta
    14341 gatcattcga agatcaaagg attaaataat attgatccac gatttttaga cttggatttt
    14401 gatccagata atcagtattc gattccatat ttttggggaa ctttagggat cgtatataac
    14461 gataaattct ttgatgcaaa tcaaattaag cattgggatg atttgtggaa gccggaatta
    14521 aaagacagtt tgatgctgat tgacggtgca cgagaagtta tggggctttc tttgaatagt
    14581 ttaggctatt ctttgaatag taagaatatg gcacagctga cagaagcagc aaataaattg
    14641 aatcatttaa caccaaatgt caaagcaatc gtggcagatg agatcaaaat gtatatgatc
    14701 aatgaagaag cctccgtggc agtgacattt tctggcgaag ctgccgatat gatgtgggaa
    14761 aatgaacatt tacattatgt gatcccacca gaaggatcca atctttggtt tgataatatc
    14821 gttatgccaa aaaccgtgaa aaataaagaa ggtgcctatg atttcatcaa ctttatgcta
    14881 gaaccggaaa atgcggcaca aaatgcagag tatattggtt attctacacc aaatgaagaa
    14941 gcaaaagcgt tattgccaaa agacatttcg tcggacgaac aattctatcc atctgatgac
    15001 acgatctccc atcttgaagt ctacaaagat ctaggatcga aatacctagg catctataat
    15061 gacctattct tagaattcaa gatgtatagg agataatggc aaaactcagt aagaatgggg
    15121 acttatcaac gttctaaaga gctaaaaatc gtcctttttc tatctgagtt ttccaatctt
    15181 ctaccaaaag cggatgaaac gccgcttttt ttgtgttatt ttattttttc taccacgttt
    15241 ttctaccaga gaaaagtgga caaaaccatt gtaaaatagg gcccgagacg ggctagtaaa
    15301 tctaagattg aaaaagttac agaaggataa agtaattgga aactatgcaa atatacttaa
    15361 cccttctgta atctattaat aaaactaata tatgaatcca gttcaatatt tgtagctatg
    15421 aaaaaacata aaaatcatat tagataaaaa ctaaattggt agataaatta ttttgtcaga
    15481 tatctgaggc aaatattcga ctatttgtgt ttcaaagtaa aaattggaat acaaatatag
    15541 aatagacata ttgcagccga tagtttatta tgcttttaga cacattaaac tggaggaaaa
    15601 aatgaaaaag agcgctaaag atcgagtttt tgatgagctg atacgactaa cagggaatat
    15661 gtcttctgtc actgccgaga aaatttcgaa gaatctaggt atctctcgtc agaatgccag
    15721 ccattatttg acgagattag tggaagataa aaaggtcgag aaattaccgg gaaagcctgt
    15781 tttgtggaag cctttagatg aatatgcggt tgtagataat acggagcaaa taaacgaagc
    15841 ctttcattct gtagttggtc atgacggaag tctgagagag gtcattcaaa aatgtatagc
    15901 cgctgtcaaa tatccgccta acggactcag tgtattaata aatggagcca cgggtgttgg
    15961 aaaaagtttt ttagccacaa aaatttttga atacgctatc catgagcaaa ttattgaaaa
    16021 agatgcacca tttgctatct taaactgtgc cgattatgcg gacaatcccg aactgctttc
    16081 ggcaacactg tttggataca agaagggggc ctttacgggg gctgaaaagg ataccgatgg
    16141 tttattggca acggccaaca acggctatct ttttctcgat gaggtccatc gcctatcgaa
    16201 agaaaatcaa gaaaagctct ttctatttat tgatactgga aactatcgtc cagtcggtga
    16261 aaatgtaaat tggcattctg ctaaggttcg ttttattttc gccacgacgg aaaaaggtga
    16321 aaattattta ttagacacct ttgatcggcg tattcaaatt agtgttatgt tgccaacttt
    16381 tgaagatcgc cccattcgag aacgattaga gctgattcaa ttattttttc aaaatgaagc
    16441 ggatgtttta caaaaagata ttattgtttc aagagaagcc ttgtctttaa tgttatctca
    16501 tccctttagt gggaatattg gtaagctgaa gaatatcatt aaaatcagtt gtgcggatgt
    16561 gtatagtcga acacaagaag aaccattaac gattgggaaa aatgagatct tgattcagtt
    16621 aaatatgctg gaatccgaag tagaaagctt gccgattgct agtttattga taaagaaaaa
    16681 tcaaaagata aaaacggatc gaatcaaacc gtttgattat gattcgattt tggaaaaaat
    16741 tactcagctg ataaatgaag gtaatttcaa tgaaaatttt tcttcgataa agaatgaagt
    16801 acagcatttg gtgcatcaca ttgataagga ttctttttta tcaatgagta gaaaattgat
    16861 gattcagatg tttagaaagg tctgggtatc cgttttagga aaaacgtacg gattgacgac
    16921 gagtggacct gttgcagaaa ttattagcag atcctatgtc atttccccaa gctataagac
    16981 agacttaacc gggataaatc gtacattagc tgcaaatcta cctcggacga cttacttatg
    17041 taatcagttc ttggagcatt taccaccgct ctcagaaaag gacgagaact ttctaagact
    17101 gcttattgtc gtgactttat ctgattatgt agatgaatcc atcgagctga agggcctgtt
    17161 gcttgctcac ggagatagta cggctagcag cattcaagca gtagtgaatc aattatgcgg
    17221 caattatgtt tttgaagcgt tggatatgcc agtagagagt aatgtgtcag agattgtcga
    17281 atcgacaagg aatttcgtga aacggcaagt gaatacagat agtctagtgc tgatcgtaga
    17341 tatgggctct ttaaatcaaa tttacacaca gatcaagaac gatttaaaag gggagttact
    17401 tcttctgaat aatttgacga cttccattgc acttgatatt ggcttgaaga tgacgatgaa
    17461 tgcgccattt aaacagattg cggaggatgc agcagacaag tattcaatca atgttcaata
    17521 ctttgaaggc ttttctcaaa gtagcaacat tatcatttcc tgtatgtcgg gtttggggat
    17581 ttcggacaaa ttgaaagatg tttttcgtca tcatttatcg gatgaatcca tcgaagtttt
    17641 cacgaaggac tacagagaac tacgtgaatt aatcgaccaa aatgatgaaa cttattttga
    17701 caagacaaaa ttggtcatca cgacttcgga tcttccacgt tccttttcaa ttcctaatat
    17761 taatgtttat gacatcttag acggtgatgg agcagccaat ttggagcata ttttgagtcc
    17821 tgaaatgaag ccggcagaat ttaaccgctt gattcaggag ctagtaaaat tcttttcgtt
    17881 ggaaggaatt gcggaccgtt tgaactttct gaacccaacg atcgtgatga gtgaagtgga
    17941 aacagtcatt tctaaatatg aaaattacta tcatttcact ttaaatggcc gtgtaaaact
    18001 aaatctctat atgcatacgg cattgatgat ggaacgttta ttcttggctc ggacagaaaa
    18061 atctgaagaa ccgaattatg atttgaaacc agatgaagat gaattttatt atgtttctag
    18121 aagtattttt caatcaatgg agatgaaata taattttaaa gtcaatgctt atgagctttc
    18181 gctgctctat gaattattag gaccgtatat tcaaaaataa agtgtctaaa aaagtgtcta
    18241 atctgttttt tttaggttag acactttttt taatgtgaat taacgtgatt aaaccaatgt
    18301 ttttaaaagt tggcacagga attgcttata taaaagtgaa cagaaagaaa ggaattaacc
    18361 atatgaagaa aattctaata gcgactcacg gtcacttagc ttcaggcttt cttagcagta
    18421 ttcaattact gacggggaag gttcaagaga ttacggtgat taatgcttat gtggatgagt
    18481 gtgattttga acaggagttg aatgtatttg taaaccaggt gaacgagcag gatcagattt
    18541 ttgtttttac cgatttattt ggcggaagtg tcaatcagaa gattaccaaa acctttttgg
    18601 agaaagagat tgctgtaact attttgtctg gctttaatct gccaattctg ctcgaaatag
    18661 tattggcaac caaggaatta acaaaggagg atattcagga actggtaaat aaatgccaac
    18721 aggaattaaa gatgaccatt cttgatgagg tacatgtagt aaatgaagag gaagattttt
    18781 tttaggagga agaaaaatga ttaaagcagt gcgaattgat gaacgattaa ttcatggaca
    18841 agtagcgatg acttggacaa agagtctaaa tttaaccggg ttagtagtag caagcgatga
    18901 ggcggctacc aacgatattc aaaagatgac attgaagatg gctgcaccat cgaatgtcaa
    18961 agtcattatt aaaacagttg atgacgcgat tcatttgtta aaagatccgc gggcgaagga
    19021 catgcgcttg atggttttag tccctaatgt caaagatgcg gtaaaagttg cgaaagcttt
    19081 cccaacggaa atcgaattaa tgaacgtggg gaatgctggg aaaatgacac cgccatcgga
    19141 agataagaaa accttatcaa aggaagtgat gttaacgacg tcagaaattc aagacttaaa
    19201 agaattagtt gaactttatc cagataccgt ttttcagcct acaccggcga tggaaaaacg
    19261 ttcagctatc tctattttga gtgattttta gaaaaaggaa ggagaaaaat tatgtatgat
    19321 gcatttgtag tcgctttggc cgtttttatt ggagttgctg gacatgaaat gttcgggatg
    19381 tccatgctga gtcgtccgat tgttgttgct ccgttagcag gagcgttgat gggggatttg
    19441 cagacggggt tgattgtcgg ggcgtcacta gaagccatct ttatgggtgt cgtcaatatc
    19501 ggaatctcta gtacggcgga gccttctttg gctgctgggc tggctacagc gttcgcaatc
    19561 aaaatgggag gaaacgtgga tgcgattatt cccattgtct ttccactagc agtattagga
    19621 ttacagttaa tgaatatgat tttttctttt gtatgtggac cgatggctgc taaatttttc
    19681 aaatttgcaa aatctggtga tcaaaaagga atcatcaatc tgcacttctt tatgtggttt
    19741 gtccactatg cgctttatgc tcttattcca ttctttgccg ttttatttgg agcagatgtt
    19801 gtcaaaagcg tcttggataa tattccagag gtcatcatga acggattaac agtcgctgga
    19861 aatctgctgc cggcagttgg gatggcgatg ctgttacgga tgctttggag taaaaatatt
    19921 gctgtgtggt ttttctttgg ggccgttgtt atggcgtatc tcgagttacc attgattgca
    19981 attgccgcag ttggaacgat tattgcaatt gttatagccc aaagagatct acaactaaaa
    20041 aatatatctg ttcatgcagt tcaaagcgat gctgctgtaa atgcaacgct tgaccaagaa
    20101 gaggaggatt tctttcaatg aaattagctg atggaatttc taaagatgag aaaaaattgg
    20161 ttcggaagat gttttggcgc tcgatgacta tgtatgcttc ggtgaatccg gtgacgatgg
    20221 gcggcggtgg tttcgcctat tctatgatgc ccttcattaa tcatttctac aaaagtgaag
    20281 aagatcgagc tgcggcgctg gaacgtcata cacagtattt ttcgacgacc attccttgcg
    20341 cgtcctttgt catggggatt gcggcatcga tggagaagga aaattctgaa aaagctggtt
    20401 ttgacgtgga aagtatcaac gcgatcaaaa cgagtttgat gggaccattg gctggaattg
    20461 gcgattcatt gttctgggga gtttggcgtg tcgttaccgc gggactagcg atcaacttag
    20521 ccaatgccgg aaatattttg gcaccaatta tctttttagt tatgttcaat atacctaatt
    20581 acttgatgcg atactatggc ggcgttttag gctattcatt aggctctaaa tatattgaaa
    20641 agctgtatgc gaacgggtta attgaaattc tgacaaaagc tgctagtatc ttgggtctga
    20701 tcatggtagg tgcaatgacg atcagtatgg tcaaattcaa taccgttttg agcttttcaa
    20761 tgaagggcga agaactaatg aatgtccaag aaatcctcga tagtattttt aaaggaattg
    20821 taccgttatt ggtaacattg agctgtttca aattactaga taaaaaagtc ggcgtcatca
    20881 aaattatttt tggaatggtc gtcttgggaa ttttattgtc attacttgga atcgcatagt
    20941 aggaagctgc agctactagg ctgcagtttt tttagcaagg agggaaaaga tgactattaa
    21001 tgaagtaatt tattcaccca aacacccatt ttttaatgct gagttaaacg acggaaatgt
    21061 gactacttca aacgaataca gctactatcg gaatattcct gatgtaaagg gattgacctt
    21121 cgatgtaagt aatcaaaaga cattagcaaa ccttgatgtt tgcgggacga ttcaaacgtt
    21181 gaccttttat cgagaaaatt tactaactga ggaaaaacca ggtgtttggg tcaataaaca
    21241 gctctctcaa accaattcgt tatccttaaa ggttgaagta aacgggattg tctaccattt
    21301 gtcagaatcg gatcatcgta ttgaagtcga tgtgattcaa gactgtttgc ctagatatat
    21361 tcatcattat tcgacgttta aggtcattgt agtatccttt gctcccatac tggctaataa
    21421 gcgtttaagt atgcttgttc aacaggttta cattgtgaat gaaacacacg aaagccttca
    21481 agtttcagtg aaagatgtgc cttcgtatca agagaagtat tcggatcagc aaaatgtgtt
    21541 gatttctcaa aaaggaaata aggacacgat cgcacaacag gaagtggcaa gcttcgccat
    21601 tgcttttgtg gacccaaatg cttttgaaga agtaatgact tttcaggaat ctgatatcga
    21661 aaaatggctg aaggaaacat tgaactattt tgctcagcta tttgggcagc tgtcaatgcc
    21721 aaacgataga gttgtccatc tctatcgtcg tgcactttat caaagctttt ctagtttcgg
    21781 tatgaatcga cagggacagg tcgtcggcag caattggggc agctatcctg ctactaatcg
    21841 aatttggaat aaggatatgt attacgcatc attgccattc accatgtttg aaccggagtt
    21901 atgtcaaaaa acggttctat ggtttgatca atatggcgtt aagtttcctg gttcaaaatt
    21961 tacaggaggt atcaatcatt ctctgagtaa ttcgttatcc agtgcgttac tggctagtct
    22021 ttattttgaa catactgggg atacaaaatt ctttgagcgg tgtccgcagg ttctactgaa
    22081 tgcttcaaga attattgagg acatattagc tcaacgtcgg tcaggggaac caatgctttt
    22141 tccatctgtt tggttatcag acgcctttgc acttggaaag tatcataccg gcagcaatct
    22201 ttgtctttgg aaggcctgta ttggacttgc agaaattttt gaagccctag aacaactgag
    22261 tcttagcaag cgatacaaaa acattgcctg taaactaaag gaatcaattt gtaaacaaat
    22321 gactatcgat ggtccttttg gtgagcaatt tttggaggga atcggtgatg aggagaaaac
    22381 aacctattca gtgaagcact atcaaaaacc gattcttgag caagggttaa tctttttaag
    22441 tgatgtgatt gtggatggaa aaattaattt attgatgcat gatggagaag aatcagacac
    22501 tacgttgatg cctttttatc aatttttaga taataaagat catttatatc agaacaccat
    22561 ggccttttct gctagttctt tcaatccaac ctatagcgag gaaatcaaag gcatcacttg
    22621 gggattagaa tccggcgcca cttttcctgg ctttatcact gttttaatga gcgagctgca
    22681 caaccctcaa gctttcgtaa cccgattaga agaactaatt tgtttggcag atttagacgg
    22741 ttcgtggtgg tggtggccgt atcgcttaga ggctcaacaa ggagaagtcg tgcgagactt
    22801 tggctgcggc aaatgcggct gggcatcggg tctttttgtc agcctaatga tcagtcaata
    22861 tttcggattg aaattcaaaa aaggagtatt gcaaattgat cctgttgata atttaacgtt
    22921 ttcttggaag aatttgaagt tcggtcaagc cttcatttcg attgaatgta cgcaatccga
    22981 gttgagtatt tcaaatttgt cagaaaagcc attgaaaata agtgatacga aaaaaattct
    23041 tcacgtagaa aagggaaaaa cgattcacat tcaaaggaga aaaagatgag tatactaaaa
    23101 aatattaccc aatttaaaaa aatccagagt cgagcaatca ctcccgaaaa tccaacaggc
    23161 gagaaaggta aagctgcgat gttgccaagc gagctaggac caagccgcaa aggtcgtggc
    23221 gcaattccgc tgacttctgg tgaagaaact gtgattgcgg atattactgg aacgggaata
    23281 attcgtcata tgtggatgac gattcgtgat cgaacagaat ttggcagttt tgttttacgt
    23341 gatttaatct taagaattta ttgggatggg tcggatagcc ctgctgttga aagtccttta
    23401 ggggatttct tttgtaatgg gtttggagag cgttgcgagg tgaactcgtt gccgatagtg
    23461 gttaatccta cagggggctt taattcctat ttcgaaatgc ctttccaaac atccgctaaa
    23521 attacgatcg agaatttaca tccgaaggat atgtcctcat ttttttatac gataaattac
    23581 gctttggtgg acgaattgga agaaaatagc ctatattttc acgcaaattg gcatcgggaa
    23641 cgcattacca aattacagga ggattataag ttaattgatc gattaaaggg tcaaggctat
    23701 tacgtgggta cttaccttgc gcttacctgc ctagaacgtt attggtgggg cgaaggcgag
    23761 tttaaattct acattgatgg ggatagtgag tatccgaccg taacctcaac aggttcagaa
    23821 gattattttg gcggggcgtg ggcctttcat gagtacgatg agttcggacg tccacatgcg
    23881 aaaaactatt caacagcctt tttagggtat ccttaccaat caaatagaga tgccacaaga
    23941 gatcgttttg aaacgggaaa attgaatgcc gttcatgcct ttggtgacga cgcaatgcca
    24001 cgacatggtc tttatcgttg gcatcttcaa gaccccatct gttttaccga agatttatcg
    24061 gtcactttac aacaaatcgg caacgatgat gttcgcttat ttgagcggca agacgatgtt
    24121 gctagtgtcg cttactggta ttcaacagaa gcttcaggaa atactaaacg tcatttcact
    24181 gatcgagaac gcttgccgcg ttaatgaagt gtttttcaca tattcgaagg gaggaaccta
    24241 tgatgatata tggggcaatt gaagctggag ggactaaatt tgtctgtgca gtcggtactg
    24301 aggaattaac gattttggaa agagtcagtt ttcccacaac aactccagtt gaaacaatga
    24361 agcaggtcat tgattttttt aaacctttta aggaacaatt ggcaggcatc ggggtcggct
    24421 gctttggtcc aattgatatt caatgcaaat caacgagttt cggtcatatc acatcaacac
    24481 caaaacttgc ttggcaaaat ttcaattttg taggaacgct aaagcaatat ttcaatattc
    24541 cgattgaatg gacaacagat gtcaatgcgg cttgctatgg tgaatatgtt gctggttctg
    24601 gcaaagggtt agcaggtgtt gcatacttca ctgtgggaac aggtatcgga ggtggtgcct
    24661 tgttcaatgg gactttcgtg gaagggttca gtcatccgga aatggggcat atgttgatca
    24721 agcgacatcc aaaggacaat ttttcaggaa actgtccctt ccaccaagat tgtttagagg
    24781 ggatggcagc aggaccagcg attgaaaaac ggttgggtgt aaaaggccag aatttattgg
    24841 ctgacgattc gttttggcag attgaagcct tttaccttgc ccaatgtgca tacaatacaa
    24901 cactgatgtt ttcgccagat agaattattt ttggcggtgg tgtaatgaaa caagaacaca
    24961 tgaagaaaaa agttcaggat aaatttgttg agcttataaa tggatatgtt gaaataccac
    25021 caattgatag ctacattatt acgcccgaat taggtgataa tgccggaata atcggcggat
    25081 tagcattggc cagaaaagcg gtaagaaata agcaaccata attaatgaat gtttcattga
    25141 tactcatgtc aaaagtattt aaccgagtaa tcaaatgatc aaatgaagtt ggataaagcg
    25201 aaaatgaatt caaataaaaa ggctctaagt caaatcgtgt tgttttcatt aaatcactga
    25261 aaggaaatgc ttctgcgttt ccttttttct ttttgttgtg cgctgtatac cttgaggata
    25321 ttcactggtg gcgtacactt aaatagtaat atattgtgtt actttatgtc tgtggtaata
    25381 gttatagttg cttaccaatg tatgttcatt tttcgaaatg ttgacttcat aatccttggc
    25441 ttaatgtttg aatttttttg gtgatagatt ttttcagcc
//
LOCUS       DAZKOB010000029        25340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000029 DAZKOB010000000
VERSION     DAZKOB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.029"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1583)
                     /locus_tag="ACG8M6_001181"
     CDS             complement(<1..1583)
                     /locus_tag="ACG8M6_001181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456862.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            1705..2289
                     /locus_tag="ACG8M6_001182"
     CDS             1705..2289
                     /locus_tag="ACG8M6_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456863.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2300..2545
                     /locus_tag="ACG8M6_001183"
     CDS             2300..2545
                     /locus_tag="ACG8M6_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456864.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2551..3273
                     /locus_tag="ACG8M6_001184"
     CDS             2551..3273
                     /locus_tag="ACG8M6_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456865.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3404..4147
                     /locus_tag="ACG8M6_001185"
     CDS             3404..4147
                     /locus_tag="ACG8M6_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456866.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            4153..4518
                     /locus_tag="ACG8M6_001186"
     CDS             4153..4518
                     /locus_tag="ACG8M6_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456867.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            4534..5064
                     /locus_tag="ACG8M6_001187"
     CDS             4534..5064
                     /locus_tag="ACG8M6_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456868.1"
                     /translation="MSQNISAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKIDFITQLTKINNLNILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFSNA"
     gene            5226..5594
                     /locus_tag="ACG8M6_001188"
     CDS             5226..5594
                     /locus_tag="ACG8M6_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456869.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            5785..6045
                     /locus_tag="ACG8M6_001189"
     CDS             5785..6045
                     /locus_tag="ACG8M6_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456870.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
     gene            6663..7463
                     /locus_tag="ACG8M6_001190"
     CDS             6663..7463
                     /locus_tag="ACG8M6_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456871.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            7500..7682
                     /locus_tag="ACG8M6_001191"
     CDS             7500..7682
                     /locus_tag="ACG8M6_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456872.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENVEDLRLIGE"
     gene            7872..8159
                     /locus_tag="ACG8M6_001192"
     CDS             7872..8159
                     /locus_tag="ACG8M6_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456873.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            8250..8489
                     /locus_tag="ACG8M6_001193"
     CDS             8250..8489
                     /locus_tag="ACG8M6_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456874.1"
                     /translation="MKEIEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHSPKVRFYN"
     gene            8617..8946
                     /locus_tag="ACG8M6_001194"
     CDS             8617..8946
                     /locus_tag="ACG8M6_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456875.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
     gene            8999..9412
                     /locus_tag="ACG8M6_001195"
     CDS             8999..9412
                     /locus_tag="ACG8M6_001195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456876.1"
                     /translation="MFSQEEIISKSQPDKNGTIIMKKKIPCYRCSGSGQYKFMSMGKI
                     TYGTCFKCDGDGFLVVTEKSFTPEYRTKLNVLNKKRADRKIRIAREKESQKNFDRYGF
                     EKGYIYVVIEKNSYTIKDELHHQGATYDPLLGWFF"
     gene            9492..9830
                     /locus_tag="ACG8M6_001196"
     CDS             9492..9830
                     /locus_tag="ACG8M6_001196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456877.1"
                     /translation="MKLEEYSKRQQEILSAKRREEGEFIGTIAQRLELDLKLTNTVTI
                     DTQWGNVYLHLFEDQNHNSLIWKTNKFLYYDEWDVGEIRKVKATIKAHDYYRDTKQTW
                     IKNVKIQKNE"
     gene            9845..10162
                     /locus_tag="ACG8M6_001197"
     CDS             9845..10162
                     /locus_tag="ACG8M6_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456878.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            10165..10308
                     /locus_tag="ACG8M6_001198"
     CDS             10165..10308
                     /locus_tag="ACG8M6_001198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456879.1"
                     /translation="MLSDLLILLIYLGGTEIIAYFLLVIGEYGISSAITQRFRLPVSE
                     LKK"
     gene            complement(10512..10988)
                     /locus_tag="ACG8M6_001199"
     CDS             complement(10512..10988)
                     /locus_tag="ACG8M6_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456880.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            11072..12445
                     /locus_tag="ACG8M6_001200"
     CDS             11072..12445
                     /locus_tag="ACG8M6_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFQ0456881.1"
                     /translation="MKKSKRKIATLLTLMVQFTGIAMSVITINPQVLHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRTVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQ
                     GQCTELTWAYMSQLYDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWA
                     VGSAGHTGVVIAVFDDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            12470..12997
                     /locus_tag="ACG8M6_001201"
     CDS             12470..12997
                     /locus_tag="ACG8M6_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456882.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            12984..13547
                     /locus_tag="ACG8M6_001202"
     CDS             12984..13547
                     /locus_tag="ACG8M6_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456883.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            13965..14702
                     /locus_tag="ACG8M6_001203"
     CDS             13965..14702
                     /locus_tag="ACG8M6_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456884.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(14719..15135)
                     /locus_tag="ACG8M6_001204"
     CDS             complement(14719..15135)
                     /locus_tag="ACG8M6_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456885.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            15528..16775
                     /locus_tag="ACG8M6_001205"
     CDS             15528..16775
                     /locus_tag="ACG8M6_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0456886.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSNPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            17045..19435
                     /locus_tag="ACG8M6_001206"
     CDS             17045..19435
                     /locus_tag="ACG8M6_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0456887.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            19484..21319
                     /locus_tag="ACG8M6_001207"
     CDS             19484..21319
                     /locus_tag="ACG8M6_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0456888.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            21394..22251
                     /locus_tag="ACG8M6_001208"
     CDS             21394..22251
                     /locus_tag="ACG8M6_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0456889.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNVNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            22241..23320
                     /locus_tag="ACG8M6_001209"
     CDS             22241..23320
                     /locus_tag="ACG8M6_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0456890.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSESVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLKVLILLIKEFRKA"
     gene            23659..24459
                     /locus_tag="ACG8M6_001210"
     CDS             23659..24459
                     /locus_tag="ACG8M6_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456891.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            24565..>25340
                     /locus_tag="ACG8M6_001211"
     CDS             24565..>25340
                     /locus_tag="ACG8M6_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456892.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQ"
BASE COUNT         9518 a         3437 c         4902 g         7483 t
ORIGIN      
        1 tattttattt tgtgaacata atttaacaat tctttctcgg aattgatttc ttttcttaaa
       61 tagtcttcag acttattaat aatattatct attatgtcta gatacatatc acttttcgat
      121 tcagctaatt catacagttt ttttaattct ttatccttta attgaaaacc ctgatccata
      181 gtcactgcat ttggatatag aagcccacta taatgtttac gtaaattcac ttggaatttc
      241 tgattataaa agtctaacag attagaaata aaaggcggaa aattactgtt tttgtaaaat
      301 tgattatttc catttaggtt gtatagtaat ttgttaaatt gctcctgatt taatttattg
      361 taaatccatg gtaatacttc catatcctta tctgctaacc tgaatgaact agcatcggta
      421 agatgttcga tcataaaatc tatgaacatc tctttttcac caaaatataa atattgatta
      481 tatattgttt ctaatgttga agatggtagc gaagaaactc tcattttttg ctggagtata
      541 ggatcaacta gactatttaa gtgattttgt aattcttcat tgttgtttcc tagaactgca
      601 agggaacgta aacggtagta atattctatt tctgttagta cttgttgttt cggtttaaaa
      661 tcatttattg atatttgtaa attttgaatt gtctcgtctg aaaaatcatg ttgtaattct
      721 ggatgggtag ctataaaagt aagtaaaaag aacccttttg tatacgtgat ctgttgattt
      781 tcaccaagtg atttattaat ttcctctctt gattcagctg tgaacctata ctttgcaatt
      841 tcttttttta gaatatttaa atagaattct ttattatcaa aaattagggc attaagtagt
      901 ctgaaagtac tttccctatt tctatctgct tcctcatttc cataagataa ttgaaaaaca
      961 aacttaaagg ctgtcttaca taggttttct ttggcttttt cgcttagttt atttagatag
     1021 ctatttttta gtgcagagtg aaattcttca ggtgtaacag acatagcatg tatgcgactt
     1081 gcatattgta taatttcgat cgttactttt tttcctaata ttggattttt taggaatata
     1141 atttcatagg acttccgtat aacatcctga caagtttctt ttgttatatc tcttaattcc
     1201 caagtattac cgttgtaagt gactacagga tgagctgaat aatttctttg atccttaata
     1261 tattttaaat cctcatattc atagctatca attagctcag ttgaactata agctttttct
     1321 agtagttcca tttcccattt tggagagttt ggatcacttt ttcttagtct agctatatct
     1381 tctaaaattt tcttagaagt attatcctcg taaatatttg ctaaatcaga taatttatca
     1441 attaaatcta aaataagcgc actgtaattg acagctattg cagaacgata atttccctga
     1501 taaaaactac ttctaacttc ttctaataat ggaataattc taggattttt aacttttttt
     1561 atttcatcat ctaatataaa catataaatc tccttttttt atttgtatga caagtttacc
     1621 ataaaaaaaa attaaaaagc gagacatatt tatgttgttg tgttatcata ataacataaa
     1681 tatgtagagt gagatgatga aaaaatggat ttgaaacaat cgttttacat ctggttatat
     1741 cctgataatc ctatgtcaat aatggctgat atttttgtag ttagcatctt attgtattta
     1801 ttttatctaa ttttctttca caatttaatc gggtacatga ttcttgattg gaaaggaatg
     1861 ttcgatatag aaagaaaacc atttgctaac aatcaaacct ataccgtctt cgaaagtgga
     1921 gattgggaag aaaatgaagt tgatgtgatc catgaaattg gattagcttt atcaggaata
     1981 caagatgctg atgttttggt atcaatgaca acggatactg gatacaacgc ttatgaggta
     2041 ttttatctta tgtattttat atggcgtgat ggtcaagaaa ttatcaatgg tcgtgctgaa
     2101 aaaacaatca attctaatac tgaaatttct ttttgtatgg gtgaagggga aatgattgta
     2161 tataaagatg ggtcagcaaa cattaaaaca gcctttggtg atattgaaac aagtattaag
     2221 gaacttaggg aatatgtgca gaacccatat aattttcaat atttagcagg aagaataaat
     2281 ttagtttagg agtaaaaata tgaaaaaaat tataaatcaa ccaacaaatc acttgataat
     2341 tgaaggtgtt attttcgtaa caatgtattt tactgtagga aatggcatct caaatacaat
     2401 cattcaaaaa aatccacaat acctattatt tagtctttat gcttggattg tacaaatggt
     2461 ttgggtttcc atattgatgc tgttgtttta ttggatagta cgggttattc ttttattgtt
     2521 aaacaaaaga aaaaaggata ggtgaataaa atgaaatcaa ctaaattgaa ggttgtattg
     2581 ttatcgacag cagcattaat aactcttaca gcatgtggta caactaatag tgctgataat
     2641 aacaaagaca caaagtctag tagtacggtg gttactaaaa acagctctac atcggcatca
     2701 acaacaattt cttcaatgac tgaaaataat gctgaaaagt atcaaaaata cgaagacatt
     2761 aataaaggtc aaaagccgga agaagtttat accgacttta ttaatacagt aaaaaatgat
     2821 gaagattatg ggaatgatcc attgcaaaat aaaaatctga ctatcgttct ttacaaacct
     2881 gattgtccag attgtcaaaa agcagaatca aaaatcatgc aagaaataaa agaaggattt
     2941 agttatgctc caacattaat gcatgatatt accataaata aaccagctta cgagtatcac
     3001 gcagttgctg tagatattac tgatggtgta cctgagtgga tacaaaacat tcctggtctg
     3061 gctgatgaga acaatcaata tcatacgcca acagttgcag tagtaacacc aaagaaagtt
     3121 aatggcacac tatactggga tgtaatggga atgacaacaa atacaactga tgaaatgatt
     3181 gaaagtacaa ctaaatatta tatggatttg ggagcatata aattatatca acagtcagaa
     3241 cctgttatgg acgatcaatc caaagttaaa tagttcttta gggcgtaagc cctatttttt
     3301 taaaattaag tgataacaaa taaatgattt ctgaaacaaa cgttcttact caactactcg
     3361 aaacacacaa gctaattaac tagattataa aggagaatgc gtaatgaaag aaatcagtgc
     3421 aaatgaagct catattccgc aaaagaatca ggttggtata tataaggtgc ttcatggtga
     3481 aggaatttat aaaatacagt tacttaccgg ttacaataac ggtgcaaatg ggaagtggtt
     3541 aaaggcaaaa agtattcgtg aacttaaaac tgaagtagaa aaaataagac ttgggttaac
     3601 aagggaagaa ataagtagac tgagtgaaaa ttcttctttt gtaatccaaa aagaaaagga
     3661 gaaacatcta cttcatcggg aactaataat tgaaaaatac tacgatgttg atttacctga
     3721 tcagttaaga attattgacg aattttcaga gaaaaagtgg aaatctgaaa tatacccaag
     3781 cttaattcaa tacaaggcat caggaaagcc catgtataac tacacaggct ttttaaacta
     3841 cacacaatat aaatatgcct ttcgcaaatt aaaaggcatt aaacaagctg acattgccct
     3901 gtatgagatg aaaaaaatgg atagattgaa attgattcaa gagttagtgc aaaatacatg
     3961 gaatcaagcg attgtcgaac tagaaaaaga aaatgggaaa attttaacta atgaatatct
     4021 gatcgtaaat gaaattgaag aattaattgg tttattttca tgtgaaaatg gtgaagtctt
     4081 cttgaagtca tcaccaatct attcaatttt ggacagtccg tatcagttta tacaggtaag
     4141 gagatgagtt acatgtctag cttgaaagat gactatatcc aagcaaaaca agagtatgaa
     4201 aaaatacaga tgttaaaatc tgagaaagtc gaattatttt taacacaaaa attagcagag
     4261 gatcatattg ctaacttaga tgctaaaact ttagaggaat atgatcagca gtttaatcag
     4321 acacatcaca atatcatttt gcttgttgat caggcaaaga gaagacttga aaaagcggaa
     4381 tatgaactat tagatcaagg aattatatgc ttggataaag ggattcgaag atattatagt
     4441 gcagagttaa aaaagcaaaa tgtagacaga catgcgttaa aacatgaaat cttagaattg
     4501 ataatgcaac gaatttgaag ggatagaaag taaatgtcac aaaacattag tgctgtaaaa
     4561 caattaatag aaaaagattt agagacgaca gttcagaggt tagccgaacg aatccatttt
     4621 gcaaattatc aaaccatctc taattggtca agcaggaaca gaaatccgga agaaattcca
     4681 atgaaatttt tttatcgaat cggaaaagaa tatggtgttg atccgataaa aattttcgaa
     4741 aagaccatga tttattacta tcatggaaca cctaacgaag tgttggaaca tcttttggaa
     4801 gaagcagaag aaaaatcatc aatattagag atatcacaaa atgacaaagc tattcaagtg
     4861 aaatgtgttg atccaacagg aaagattatt gttggatttg atattgaaag aaaatctaaa
     4921 atagatttta ttactcaatt aacaaagata aataatttaa atattttaac acttagacca
     4981 gatcatgaac agttgtttat gagttttaaa aaacaattga aagaaataca aaaaagatat
     5041 aacaaggtgt tttctaatgc gtgaaaagct aaaagaaatc agagtgtttt ggataattaa
     5101 caaagaattt attatcttgt atacacttgg atttatcagc atgatcttgt tttggtggtt
     5161 atatttaaaa aagtaattta aaagtgtcaa ggagattttt atccagagaa ggatgtggaa
     5221 tttgcatgtg gaataataaa ttaaaaacag gcttgttatt aatagttatt tcctgcgcta
     5281 tgatggttgg catgataata caaagggaac aatcttattt tgaagtttct gctaacaatg
     5341 taatagaaag atgccattat ggtcaacatt tttggactgg ggaagtcaga gaaaatatag
     5401 atcgtgagta tgttcaaaga atcgtttggg atgcatattc tatcaaagat tatcctaaaa
     5461 gtttaacgag tagattattt tacagcgaaa aagataatca aaaactctcg aatttgatga
     5521 tgaaaaaagt aaggaaactt gcacaaaact atttggaaga aaaagaaggt aatagtccaa
     5581 ttgacaaaaa ttgatgacac tgcaaaaaaa tatagagttc gaattagctt ttttcggagc
     5641 attgtttttt aggtgaacta tatttcatca ctaaaatcag agataactaa cttgaaacat
     5701 ggtcagatgt gttacttgaa gtctctgaat aatcaatgtt atctagtaga attaagcaac
     5761 taaaaaagaa agaagtaaga aaatatggca tacgaaaatt tacaattagt agtcgcagaa
     5821 gttagtgggg atatatacct tacaaaagta aaagatggaa ttatggacac gaattatagg
     5881 agagttgcta cagatgatgt gctaagctca tcgacagaat ggtttcgaaa aaatgaagaa
     5941 tctgctgttc actttgaagg aattgatgga aacattcata gtttgttcta tacaaaagac
     6001 aaagaaaaag caaaaaaaat tcttgatatt ttgaaaggaa ggtagttaat taaccaatga
     6061 tataagagaa tgtgagacca agtaaatact tggtctctat aattttagcc actagtcaat
     6121 taaatagcta aacaatttta ataataataa aatatgactg cttcataatt aagaagtgca
     6181 gaatttttct aagaacgata tttccttgtt gaaatgagta ttgctaacaa tttgatcacg
     6241 aacagcatcg ttacaaatgt tgtatcaata tttgatagat tacatattct attaaaaaag
     6301 aaaagaaaat tattgatatt atacacatta gtgtgtataa taaaagagag ggagtgatta
     6361 gaactttgag caattcacta attgtttaat aatagaacga ttcctaagaa gttcattgtt
     6421 cattggcaaa ttgctttatc agattgaaag ttggcgaata aggtttgtag aataacacct
     6481 tttcaagcaa agcgattttt gaaagctatg aaatttgttt aataaaaaaa ttcaccagat
     6541 aatctgatag agactaaccc aattactaac agttgtaatt ggtgttagct tacaaattaa
     6601 ttattgtgtt tagtcaatta caaattgtga aacggcacta ataattggaa aggaaaaatg
     6661 aaatgtctaa agatttacga gagcttaaat tgcgaaaaaa gcaatataat caatattttt
     6721 ctagtttgtt gaataaacat attgaagaag gacttgatca tgattttgga aaagatgaat
     6781 attgtttata ttgcgaacgt tacaatgaaa aaatgtctga atatctacag ctgaaaaaag
     6841 agatacagaa attagagaaa aaagtacatg cttggaagta taaagattcc aggtctggtt
     6901 atgttgaatc tcaaaataag ccaagaaaaa agaagcaagc gtatgttcta aagatggtaa
     6961 atggggaaga gttaattgta ttagcgtaca gtgaagaaga ggttcgagaa ctcgttgaaa
     7021 cacctgtcag gaaaaaggtt gaaacaataa atgagataga cgacagccaa tacaatagca
     7081 tctatattga aacaagtcat ggtggatata agaaattagc aactttcctt agaagttcta
     7141 aagttgggat aatttctaaa aaagacataa gtgctgttac cgatagtttt tgtactttat
     7201 ctgttcctaa agcttatttc caatacgata gtaaaagatt tgaacgctta gagaaattct
     7261 gtagaccgta tactgtattg aaaactaaaa aatttaataa aactaaaaga gtttcgatac
     7321 agttaccgac tggtcaaatc tcattaggtg aaaaagacta tctgataaaa gtaggaaaag
     7381 agttaattat aaaagtaccg gaacacttat ttaaagaaaa ttttgagata cgtgaaacct
     7441 tggtatggaa agaagaaaaa tagtttggaa gacatagaat ggtataaggg gaaattgaaa
     7501 tggcagtaaa taaaatattg caggtattaa tcatagacga atttggcgga ggagaatggt
     7561 ttgatgtgca gtccatagac tttgaagaag aagtcgtagt tatagaaaat atttacggcg
     7621 aagtatactc atattcgctt gaaggtgaga atgttgagga tctacggttg attggtgagt
     7681 agaaaaaagc agaaatatca aaatggagat aaaaattaat agcatgtaaa gatagtcatg
     7741 cttacatgct ttatccaaaa tggtatgcag acaaaaatat agctgaaata aacgattatc
     7801 taactagtat aaaggaacgc aatgcaatta attaacaata gacgaaaaat gaaattaaag
     7861 gagataggaa aatggtagaa gttgaatttg gatcaaaaga atggtttgaa gagttaaact
     7921 caatgattaa gttcccagaa aagtttactt tttactacaa taaaagagac aatatcattc
     7981 aagtagcttt acttgaaact aagggaagat tagaaaatac tcgatggagt atgataaaac
     8041 catcggcact tagatttcat cctaaagact tgacagcggt tgtagcggta aaagaggttc
     8101 aagactcaca agaatattat gaagtagtaa aaaatctgaa ggatatttgg tgtaaataat
     8161 tctaaattga ccagttattt ggtcaaaaat agtatgtgat tatttcatcc aatgtggcat
     8221 cataattggt atggaagggg ataaattatt tgaaagaaat agaaggtatt aagaaaagaa
     8281 atcaggaaag attggaactg ttaaatgaaa atagaaatag aatgtttagt tattatgaat
     8341 ctatgaggtg tttaaataaa tctagaagtg aaattataga atcatcaaag tataaacaat
     8401 tgatggaaga gagagtttct ctcatccaag aaatagaaac agagtctatt tttccagatc
     8461 attctcctaa agtccgattt tataattaaa cattaacaaa tgaaacgatg ctaaataggc
     8521 atcgtttttt atctatttat aaaattaatt tatttacaaa atgtgtatcg gtatgtatgg
     8581 tgaatttgta gataagttga gaaagggtga tcttaaatga aattagaagg tagcttacaa
     8641 aatagaatga tggaaggcag tacaacaaac aaggagattg tagttggaat gggagcaacc
     8701 gagcttctat ataccgacag aaatccttat acagtaattg aagtaaaaag caaaaacaga
     8761 attttggttc aagcggatgg agcaatcaat aaagctactt ttccagatca agagtgggaa
     8821 tatagcagaa atcctgaagg tcaaatattg gagttagtta agacaaaaaa tggatggaaa
     8881 gtgcttaaag aagatattca tttttatata ggtgatagag aaaaatacta cgatcctagt
     8941 ttttaaacaa cctgaagatg agcgaggttt tatcctcgct cagaaaggag tatggaggat
     9001 gttcagtcaa gaagaaatca tcagcaaatc gcaaccggat aaaaatggaa ctataatcat
     9061 gaagaaaaaa atcccttgct atcgttgtag cggatcaggt cagtacaagt ttatgtctat
     9121 ggggaaaatt acctatggca catgttttaa gtgcgatggt gatggatttc ttgttgtaac
     9181 tgaaaaatcc tttacgccag aatatagaac taagctaaat gttttaaata aaaaaagagc
     9241 tgatcgaaaa ataaggatcg caagagagaa agaatctcag aaaaattttg atcgttatgg
     9301 ctttgaaaag ggttatattt acgtggttat agagaaaaat agctatacga tcaaagatga
     9361 actgcatcat caaggagcta cttatgatcc tttattaggt tggttttttt aaggaaaaac
     9421 aagaacaata caaaactcaa aaagtttttg tagaagaatt ttctgtaatc aatgatgaag
     9481 gaattattga gttgaagcta gaagaataca gcaaaagaca acaggagatt ttaagtgcga
     9541 agcgtagaga agagggtgag tttattggta caatcgctca acgattggaa ttggatttga
     9601 aacttaccaa taccgtcacc atagatacac aatggggcaa tgtttacctg catttatttg
     9661 aagatcaaaa ccataattca ttgatttgga agacaaataa atttctttat tatgatgaat
     9721 gggatgttgg agaaataaga aaagttaagg cgacaataaa agcgcatgac tactacagag
     9781 ataccaagca gacttggatt aaaaatgtta aaattcaaaa aaatgagtaa gaagggataa
     9841 aagtatgaaa agagaagaat acaaacaaag attaaacgaa ctgcttgaag aggacgaaac
     9901 attaactcat ggttcgccgg atgaaattct atacatgatt gataatatgg taatttttgg
     9961 tggatatgaa cttggtaata ggtcagttga tcataatatt ttagaatttg atgatgtatc
    10021 atgggaagaa attcttgatt ggggaatttt ggcagtacct gaaactaaaa cctatattag
    10081 tgacactatg gttccttttt ttgaggaatt ggattataaa agacttccca aaaatgaaaa
    10141 tcatatactg ggaggaaact aacaatgtta agtgacttac ttattttact gatctatcta
    10201 ggtggaactg aaattattgc gtattttttg cttgtaattg gtgaatatgg tatttccagt
    10261 gcaatcactc aaaggtttag attaccagta agtgaactaa aaaaatgatg agattatcag
    10321 catatctttt aatgtttttg aatgtggtca tgttaatctt tgtagtaatc ggtgtgactt
    10381 tcaatttaac ggaatagagg gaaatctata atggaaaaaa gagaaaagaa tgaacatatt
    10441 ttttgagtca atattgttga tggtttttgc gatcagcgct taccagaaat ctaaaaaaga
    10501 gtaatgactg tttagtcatc actctttttt tgtagggaaa ttgaaatatg tggagcttgt
    10561 attggtttag ttaggattgc ttggctataa ggagctagtt ctttatcaaa accatcaaag
    10621 taggttttca gtagcgattt aactgatcgc tcatcttgtt tgatttggat aatttcagca
    10681 tccaaatcgg ttgtgaggtc aggagctttt gaagcgatta cctgattttt tttatcttcc
    10741 aactttctta ggctgtttaa caagttgtaa aaataatcat tttgactgtt cattccaacg
    10801 tgagatgcaa ttttaacaga agtttcatcc gtattggcat caagtatttc atcaaaagaa
    10861 ctcattggaa tatcaagtag taacgaggat gtttccttta cagcattttt agtttttcca
    10921 tcttttttgc tcttaacttg agatattttt ggatctagtt taggtaaatc acttgtttta
    10981 tcagtcattt attagctttt cctttctatt aatttatgtt attatattat cataataaca
    11041 taaaaaagaa cactttttgg gggcgtagtt attgaaaaaa tctaagagaa agattgccac
    11101 gttgcttacg ttaatggtgc agtttacagg gattgcaatg agtgtaatca caataaatcc
    11161 gcaagtgtta catgctgatg ggatagctac ttatgatgca caatttaact gtactactga
    11221 aggtagtggg tcaggaacag atagcgatgg aaacgatact ggaagtggcg ctgtaacagg
    11281 tgatgtagcc aaaaatattg aagatattta caaatacatg aatggagaat atggcttatc
    11341 aggtgctagt attgcaggta ttttaggtaa ctggaaacaa gaatcaggta ttgatcctcg
    11401 aacagtagaa gggtattacg gcgatgggca gagagaagtt gacttcatca aacaatttac
    11461 tgatggtcta atgggtattg gctatggtca atggacagcc gggcgacata cagcattagt
    11521 tgattggtct aagaaaaata atggcggttc ttggtggact acagaagccc aattggactt
    11581 tatgttcaaa ggtgatgggg gattcgtagg aatattaaaa gattatgctt taaatgcttc
    11641 tgatgatgta gtagaaaatg cagtaacttt tcataaaaac tgggaaattt cagctgattc
    11701 cgaatcagct gtaatagctg tacgtggtga aaatgcaaaa gaaatctgga agtacatgaa
    11761 aagcaagggt atggacggtg caaaagatga aagtaagatc aataaaatat ccggaggttc
    11821 aagtaccgga gatggatcga ataattcatc aagttcagca tcaggaacag tagaagatgc
    11881 ctgtgcaact acggatgatg ctaattctgg ttctggatca attgggaaag aaggaaaaat
    11941 taacggtaaa aaaggtcaag taatagcaaa tatgacttat gaagaagcat taaaaaaata
    12001 tgggaaagca atcacgctac caaaatttaa agaaccagac tggtcaacat caccttttca
    12061 aggtgaatta caaggacaat gtactgaatt aacatgggct tatatgagtc aattatatga
    12121 tggaaatcaa ccaactttgg gaaatggcgg ttacgtttgg gaaagctaca aatcggcagg
    12181 agcaaaggtg acgaacaaac caattgtagg ttatgcgttt agtgctttag atggatggtt
    12241 atgggctgtt ggatcagcag gacatacagg tgtagttata gcggtgtttg acgatggatc
    12301 atttttgtgc gctaatttca atgttccacc tacccaagca ccaacaagag gtgtttcagt
    12361 aactttgatt gatggtgtgg atgggtcaga taatgtgcat ttctttagcg gtgtaggcaa
    12421 agctaaattt gaaattgaaa aataaaaatt tgaatggagg gcgacagaga tgaatagtaa
    12481 aacgaaagat gcaattatca tttctttgct ttctggtatg ctattagcaa tgcttgttgg
    12541 aattctatat ttcaataagc agaatcaaga catagaagct gaaaagcaca gtgtgcaaat
    12601 tgaaaatgaa ttgaccttct ttaaagaggt taaaccaaat gaagtggatg gattgcaaag
    12661 agatttagaa aatgatattc aagagatgtt taaaggatca gaccgaagca aatttgcgaa
    12721 tcagtcaggt gaagaatttt tgcgaaagtt atttattgtg acaggtaagc caccagttac
    12781 taacgagagt agcgagcaag aaaaaataga atactataaa ccttttactt ttaagctaac
    12841 aaactttgga attaagacat ctagtagtgg gttgtatgaa gcattgactg atttcgaagt
    12901 tcaatacaac ggaaaaaaag tgtatgatac cacattaacc atcacactaa ataatgataa
    12961 gaaagtgact gggggtgctt tttatgcaaa atcatgaaat tactactgct aaaaaggcga
    13021 tcggaatact ttccataatt ttgatccttt gttgtgtggt actatttcaa aagaaaaatg
    13081 cttatgataa taatgtcaaa gaattggaag accttaaaag taaacaaatc gaagtagtca
    13141 agaataacca gaaagtgaaa gctatgcaag aagcccaata caaagaattg ggaaatgctg
    13201 aaatcaagga acaaagtagg aaattcttta atttatttta caactggaat tcatgggaaa
    13261 aatatacgaa gaatatgaag gaaatacaaa aagaatttcc tcagatagta aagtctagtc
    13321 ctgttgatat ctctggcaca atggtaggta ctggtaatag tccaatatct agctatactg
    13381 ctgattacta tacaacaaat caaaaaggta aaatggttga attgattaca cagacaagaa
    13441 atactccaac aacagcaaca acaacaattt ggcgtggagt aacaagcata ggagaaaatc
    13501 aattgtttta cttggaggat ttagatagct atgtaagagc agagtaaaat aaaaaaagag
    13561 ctaacggtac caacgttagc tctttgtgct gacgatgata acaaaaccta ccgaaaaagg
    13621 attgtttcat cacaaacaca aacagaaaag aattgataaa aaacccgaac ctcagaattt
    13681 ttattaatca attcattttg attataataa attgagctga acattacaat acgtatatgt
    13741 tgtaaaacgt aaaaaaagtc aaaagtaaat actttcttaa aaagtcaaat aaaactatta
    13801 aagacaatat atatggttta tttgacataa gaaaatggtc tagatgataa ttagaatata
    13861 gaattgtaga cttaaataaa tcataaaaaa ccttctatga taagttatag ctcttaattt
    13921 taagcagttt tagatataag aaagtaaagt gggggagaat aataatggca gatcgtaaat
    13981 tagaaaagtt attagaagaa acgtgggatc caaaagaatt tagtgaattt tttatggaga
    14041 attttgaaac tgacttagcg gtgattgtta aagatgctct tagagagcaa gggtatcctg
    14101 aaactgcaaa ttatataaac attaatttta ctttgtatac tgaaaataga ggaacttggg
    14161 atttttgggc taacttagca aataaggagt taagtgataa atcagataca ggaattcgta
    14221 acttttttga atcaaacaga gatgattata tgtacgctaa caatcaaaat aaattgaact
    14281 ttagagtaga gtttgatgaa acacctgaag aatttataga aagacaaccg cctaaagaaa
    14341 atgtagcaaa ggtattggaa gatcgttgga attcagatga aatagtttca acaattagcg
    14401 aacaagacgg acaatatgaa ccgctagtag aagcggttag agaagagcta agactgaata
    14461 agtttcctga tgttcaaaat attgatgttt ctcaaattga aataaacgtc aatatcacca
    14521 atagacttga ttatgatagt tgggcagata tagcattaga aaaatatatt tactcaacgt
    14581 taaaagagtt tatcgaaaat agaatgaata tcatgtatct acagcatcca cagtatttaa
    14641 actttggtgt tgaaatagct acacctcttg aagaatggaa aatggagcaa gggcttgact
    14701 agcctttgct ccattttttt attttacaaa tgaatacagt tcttcgtctt taccaagaat
    14761 gattgtttgc ccaactttga gattatcagg tgatccttca gcggtttgta aggagaacgt
    14821 tccattgaac ttatcggata ccgttatagg tagctttaat tctgatcctt cctcatcttt
    14881 gagaattgct gttttccgtt catcaaaata aacagtcttt attagataat ttttagtagt
    14941 ggtttcatca gatttgtctt tgtttttagt agtactaatt tctttagaat tagatgtatt
    15001 ttctatgact atttcgtttt tggtatttgc gcttgtattt atttgattga aaattttaat
    15061 cgacaagatc ccactaagaa tagccaacat agaaacaagt gtaattaata ctgataattt
    15121 cctttttttg ttcatttctt cttaacttcc ttttttgttt tgattagtta gatattcctt
    15181 atctaataag cctttttagg ctattaaatg aagataaacc ccaaaaaatt attgcaataa
    15241 tgatagtgca atggtactat tattataaaa taaattttgc gaaaggggta ggtctaaatc
    15301 gcaaataaaa agaaattgaa cctagacaaa tacaacttgt ataccgcaag ggaagcaagt
    15361 ctaattttgg gtaaaggtag aaattatgtt acaaacataa tcaactatta tccaaatcgt
    15421 ttgcctaaag gttcgttcaa gaaagtgggt gacaaaacct atattataac ggaagaagga
    15481 atagaagcat tgcgatcaca aacagaaaat aaaaacaaaa agaaggaatg aatatgaaga
    15541 aaaattcgct tgctatggtt ttatttgcat cagctgtact tgcggggtta tcagtaccag
    15601 taacctcaca tgctgatgaa tggactgcaa acacaccgga tcaaatccaa attacaacag
    15661 gacaatcaag ttatgcgttc aaaaaaggag atactatttg gtcaactgta gagaagctta
    15721 aaagcttagg ttacaagact actgttgctg agtttgccga attgaacaat gttgatttgg
    15781 caaaaggaga acaacggact atcccgattg gacgaacttt caacatcaat aaagatacat
    15841 taacagttaa ggaagcagat ggcgaagtag ttagtcaaac tgttcaaaaa gataaacctg
    15901 atcctgttga aaatacggag caagagaaca aaaaatccga gactacaaaa gaatcggaac
    15961 caacagcaaa cacttctgat aagacagaaa caagtgcttc aagtgttgat ggagcaagtt
    16021 catctaatac aaataattct acaagtgaga aagaagcatc acagggaaca agtgaaagta
    16081 gcactacgga agctgatact ggaacatcaa caaataactc acaatcaaca tcaaatgatg
    16141 caaatgaatc aaccagtaca tcttctagtg atcaatcaca aacaggttca agtgatgaaa
    16201 ctgacagttc tacttcacca agtaaccccg aacaaccaac taatgttgtg ctaggcgttc
    16261 catttattag tcaaggtaac acaatgcttt gcgaaggaac atctttgcta gaagcgttac
    16321 attataaagg tgttgcaaca aatcaagatt taatgacttt tgttaaatca atgccactat
    16381 ctcctgataa taatccaaat aatggttttt ctggtgaatg gagacacaat gtggatggaa
    16441 cttatcaagg tatgaatccg gctcctgtag tttcatgggg tgttgctaat ggcggaaatg
    16501 ttgtagatat ttctggttca tcggtacaag gattaaaaga tgagattgta aatggaaatc
    16561 ctgtagtagc gtgggtaact tatgcctttg aacctgctca acataaatca atgttttggg
    16621 gcgatgctat ttggaatcga catgtagtag ccgttgatgg atttaaagac ggtttttacc
    16681 atgttgtcga tcctgtgttt ggggcaagtt ggattaatgc aggatcattt gaaacagcat
    16741 ataacgtatc tcaattagcg gtttcggtac gttaataaaa gagatcgttt atatactaag
    16801 tagttaagta tagtctttat ttgtccttgc ttaactactt agaaaacttg ttgctcatgg
    16861 ttggtagaac taaaaggggt gatgctatga gtactagagc gttgaaacag cttgctttca
    16921 acgaaaaaag tgattaatag gtgtggtgat tttaacttag gaatgggcgc gaaggtcatt
    16981 accacaccga catatcgtgg ctcattttct tcgcctaact ggtataaaga aggagtaacg
    17041 agagatgaag catgaaaaaa cgataatttt aaaatcaaaa ataatttatt gttttgcgac
    17101 tttagtaatg gtgttatcta cgcttataat gcctttttca gcaataagag ctgaagcaga
    17161 tgtcacaaaa ccgacaagtg caaaatttac agaaactggc gtaaaaacag acggttatat
    17221 tagggttaat atgccgaatc atccaaatga atggatgatt tcaagtcaat ttaaagattc
    17281 tcatggaaat attggatatt gtatggattc agaattgcca tccccaacag gaagtggagc
    17341 cggaagcttg aagtacaagg gagctggcag tgatgagttc tatcgtatgt ttaaaggtgg
    17401 ttttccatca aaaacagcaa aggaactggg tgcaggaaat gatacagaag catggtatgc
    17461 aacacaatta gtttcatggg ttttagccgg aaactttaaa gtttcacaga ttgtatggtc
    17521 acatccaaac cataccgccg cagaaacagc tcgtgtaaaa aaagcatttg aaaaaattta
    17581 tgactatgct aagaatggaa aagatacgcc caacactgag ttttctatca cagcttctaa
    17641 gacagctgac gaaggaaaat atcacacttt cacatataaa acagcatcaa ataagactgg
    17701 taatgcgaag ctcactttta catcagcaaa accagctggt atgaagattt atgatgcaga
    17761 tggtaaagaa attactaaca acactgtgaa attaaattca agttttacta ttaaagtacc
    17821 tgtgactaca ccaagtggta ctttgtcatt taaaggtact gctaatgtta gcacaaccaa
    17881 tccttttact tttgatggac gaggtgtgta tcaagatgcc gtagttatga tcactacttc
    17941 agaaacaaag gactctaaat ctttgagtgc taaatggaca agagctgttg gtcaggtaac
    18001 agttaagaaa gttgatcaag atggaaatgc tttaaaagga gcggagttta ctctaactga
    18061 caataacaat gctaaatctg ttaagacaac cggagatgat gggttagcta cgtttgattt
    18121 gcaacaaggc ttaacataca gcttagaaga aacaaaagtt cctgaaggtt atcaaggttc
    18181 atttaaaaag gatggtatta ctgttaatac cgatggacaa gaatttagtt acactgctac
    18241 gaacacaatt aaaaaaggta aagtaaaagt taaaaaggtt gaccaagatg gaaaaccatt
    18301 agctaatgct gaatttacct tgacggatga ttcatcccaa acacaagcag aaaaaacaga
    18361 tgaaaacgga cttgcaagct tcgatattgt agcaaacaag acctacagcc ttgaagagac
    18421 cggagtacca actggttata caggcgtatt caagcaagaa ggaattaccg ttaaagatga
    18481 tggacaaact tttgaatata cagctacaaa cactttgaaa aaaggtttag taaaagttca
    18541 taaagtcgat caagatggaa aacctttggc aaatgcagag ttcactttaa ccgatgaaaa
    18601 aggtgcaaca caagtagaaa aaacagatga aaatggactt gcaagcttca atattgtagc
    18661 aaacaagacg tatagcctag aagaaactgg agtaccagta ggttacacag gtgcatttaa
    18721 gcaagagggt atcaccgtta aagacgatgg gcaaactttt gaatatacag ctacaaataa
    18781 tttgaaaaaa ggtgtagtaa aagttcataa agtcgatcaa gatggaaaac ctttagcaga
    18841 tgcagaattc accttaacgg atgaaaaagg tgtaaaagaa gttaaaaaga cggatgcaac
    18901 tggtctggct acgttcaaca ttattgcgaa caaaacttac aacttagaag aaacaaaagt
    18961 accaacaggt tacacaggcg cattcaaaca agaaggaatt accgtcaaag atgatggaca
    19021 agtctttgaa tatactgcta aaaacacttt gaaaaaaggt gtgatcaaag ttcataaagt
    19081 ggatgatcaa gggaaagcat tagcaaacgc agaattcact ttgacagacg acaaaggtgt
    19141 aaaagttgta caaaaaacgg acagcaatgg cttggcaaca tttaatattg ttgccaacaa
    19201 aacgtacaaa atcgaagaaa cacaaaaccc taatggttat acagggaaat ggtctaaaga
    19261 aggcatcacc gttgctgacg atgaacaaac ctttgaatat acagtaatta atcaccttgt
    19321 acctgaaaca gtaagaacag gcggaatcgg tacatcagcc ttccttcttg tatttctaat
    19381 tgcaggattg agtatggtgc tactgtatta tcgagaacag cacaaagcaa aataaaaaag
    19441 tgttaatgga aaagaagtaa atatgaaaaa agaaggctga cgtatgaaat caaaaaaaat
    19501 tttttcaaat ttaatgctat ttggtacttt gctaggagtg ggattgggcg caagctcaat
    19561 ctcttctagc cttggactag gggggcaagt agcatatgca caacaaaact ataattatca
    19621 aggaactgga acgattcata tccataaagt aacaaatact aatggggatg ggaaccctgt
    19681 ctcagatatt gataacaaag ggaaagaaat agctttacca aatggtgtaa aacctcttga
    19741 aggcgttgag ttctcttatg tgaaacttac taaagatgaa gctaaatcct tgggatggac
    19801 ggatagtgat actacaaaag gtcaacatat tgatgatgct aaagcacgat tattactaaa
    19861 caaaaaatac agtgaagtaa gtagaatgaa gactgattca caagggaata tagacatacc
    19921 tatacaagta aatacagacg atttaacaga taacttgtat ttgattgttg aaacaggaac
    19981 agtcaaaggt gtagttgaag aagcgtatcc tatgattgtt aacgtacctg aactaaacgg
    20041 atcagatgat caatacttta attatgatgt acatgtctat cctaaaaact atttccaatt
    20101 aaactcagca gagcttgata aaaaagacga agatggaaag gctcttgccg gaactgtttt
    20161 tgaattgcga aaagatgatg gatctgatca ggttgttgga acttataaaa cagacagcac
    20221 aggtaaatta actgtagatg gtttggaatt aggtgactat tatttcctag aaaccgactt
    20281 gggaacagat gctaataaaa caacctattt actagatggt aaaaaattca aatttagcgt
    20341 taaacgtggg gagactgcta aagtagttct tgatgttacc aactacaaac gacctgtaat
    20401 tactaaggta attgataaag aaaccgttga tactggcgaa gatgttcaat atacattgaa
    20461 accatcaatt cctggtaata ttggtaatta cgaaaaatat gttgtcaaag atgttctagg
    20521 tgcagagtta acttatgatt catcagaagt gatcgaagga atgactttag atactgatta
    20581 cagtatcgaa cgtgacggac aaacgacaaa ctatgtcttt actgaatcag gtatggataa
    20641 gcttcagaaa caatttgatg caggggatcg cttctttaca attttgatga acgctcatac
    20701 taatagtaaa gctaaaatgg atacaccact taagaatgat gtttcaacga tctacaacaa
    20761 tggatatgaa cccgaagacg aaacaccagt tgatcacgct tatacaatca ttggtgatca
    20821 ccaattcatt aaaacagatg gagatacaag caaagcattg agtggagcaa aatttgtagt
    20881 gaagaacaaa attgatccga ataaagttac ttacctaaaa caagatccaa caacgaaagc
    20941 tgttacttgg attgcagata aatctaaagc tactgaattc gtttccggat cagacggtaa
    21001 gttccaaatt gaaggcttgc aatataccta tgatgtagat tttgataaac aaggtaatcc
    21061 aacaatcaca gataaaaaaa tcaatgatta tgctcttgaa gaagttgttg ctccttcagg
    21121 atatacacca actagaaatg atgttccatt tgtggtatcg aatgatgatg cttctagcga
    21181 agtaacagac atcaagaacc ttaaaacacc tgaaacagtt cgtacaggtg gaattggaac
    21241 agtagtattt ctagtggttg caggtgtggc aggaacaggg ctatatgttg tctacaaacg
    21301 agaacaacac aaagcttaat gaaataaaat tccctagagt gtttttggct ctagggtttc
    21361 tttttaaaaa gtgtgtgaaa agtaggtatg agcatgaaaa agaagaatat caagttgatc
    21421 atcttaaaaa ttgggatgtt gctatgttta ggagtaggtc tatatcctgt aattactcag
    21481 gcttatacct actaccaaac tcaacaaaca ctaaactcct ataaggaaaa atatgatgaa
    21541 gcttctgaaa aggagaatga agaagtcttc aagagatatt cagaagttaa taggaaacga
    21601 aaagacacag aacaaacctc agtcacgatt cctgaagata ataacaatat tccgtcaaat
    21661 gtaaatcttc agaaggataa attaggtgaa ttagtgggtg aaattcttat accaaagatt
    21721 aatgtggatt tgccaattta tgatggtgta aacgattttc aactatcagc aggagcagga
    21781 atattaaccg gaactgatta tccaactggt gggattggaa accattctgt tatatcagct
    21841 cattcgggca ttcctaataa aaaatatttt acagatttag agttgctcaa acttggtgat
    21901 aaatttttta ttgatatttc agggaaaaag cttgcttatc aagtagatcg gataaaaaca
    21961 atcctaccaa ccgaatttga ggatttgctt cctgtggcag gaaaagatta tgtaacactt
    22021 atgacatgta cacctgttgg agataattct cataggttac tcgttagagg ggttagcata
    22081 ccttaccaaa aaagtattgt tgagaaagaa aagaagaaag aagatttaaa ggtaaaggcg
    22141 cagatgaact ggtatcctat cgtagttgta ggggttttat taataatagc tgtgatagtc
    22201 attattaaaa aaatagtagc aaagagacga ggaatgaaaa atgaagaata aaatactact
    22261 ttttttgact cttatttgca ctttggtttt aacaatttat cctgcaaatc agctctatca
    22321 tcaattcaaa acaaaagaga atgtggcgga aatccaagag aaaattgaga aaaaacaaac
    22381 tccttcaaaa gaggaagtgg caaaaaaagt cgaacaaata aaatcgaaca gcggtaacca
    22441 aggattagac aatactcaga aaacagacgg cggatcattt gatattggtg aagctgaagg
    22501 aaaggtcaat ccttacacag gagatgtttt ttcaaaaaat caaacgaaag attttgaacc
    22561 atctacctat tcaggggcgg ttggttatgt ctatattcca aaaataaatg tgctagagct
    22621 tttgtttcta ggcgcttcac aggaacattt atatagaggt gtggcacaga tgagtgctac
    22681 tagtctacct ttatcaggta aaggtcatca aacagtaata gcaggtcatc gtggcggtta
    22741 tacgtcacca caatttcttt atgttgatca attagtagcc ggagaccaca tttatataga
    22801 ttatcttggt gaaaaagcag tttatacggt tttccgaaca gacgttatta gaaattacgg
    22861 tcagaattca acactgactg atattcctga tttagatatg ttaacacttt atacttgtca
    22921 tccatatcct cagaactacg atcgtttatt agttagaagt ctaagagatg aaaaagcaaa
    22981 atacatcacg ccacaagtaa cagttggcaa taccgcatac gctgatgcca ttgatagcga
    23041 gtccgtttca gataagacag aaactgttac atcttctaat gatttatttg aggatgttga
    23101 tcgtgccaac aaggaattag atggaaataa tgatcttaat attccggaac aaggtagcgt
    23161 agagatgcaa gacagaacat ttgctgaaaa ggcaagttca atattcacag gaattaccaa
    23221 taaacatcag aaaatgcttt tagtcatttg gactgtaata ggagcattag caagtctaaa
    23281 ggttcttatt cttttaatca aagagtttcg aaaagcataa tgaagattat aaaaattgat
    23341 agagtacagt aaaaagggat tgatactatt attgatctct tttttatttt gaatagagct
    23401 agattcactt ttaaataaaa tcaaaactga ttttaattct aaagaaaaag tcaagaaaaa
    23461 aatacaacaa atttttttat cagaatattt atcagaatat tattagaaaa gataaactca
    23521 aaagcttaat ttaatagggt tattggttgt atttagaaat aatgtatgga aaagctataa
    23581 agaaatagat aagatgtaga aaatcttatt tgtgaatgaa aaacaagtca tcgtaaaata
    23641 ttaataggag gtgtagtaat ggtgggaata tcaaaaacag ctttagactc attagctaaa
    23701 ctatttagta gcaggtttga tagtgcagaa tttgttgaat atctaagcca aaacgaaagt
    23761 ggaaaaactc tgatcgaaac aatgaaaaaa gactatttga atgaaaatga ctttccgaca
    23821 tggacgaata ttgaagatgc tacatgggat gtagtgtgta cgatatattc tccttgggta
    23881 acgattgctg attttgtttt agataaaatt ggaggggata cataccctga tgccaaatct
    23941 cttgctacaa gtgactttaa aacgttctat ggttatctaa taggtctaat aggcaatgga
    24001 atagatttga aagttacgcc aaaacaaaca cctgaagaaa ttgaaaaaga aaaaggagag
    24061 gttcaaatgg cttatgcaaa tgacagagaa aaagtaaaat tattggaagg taaattatct
    24121 gaagaagaac ttggaaaaat ggtgcaagaa atcaatgaag acagcaacta ttttcctgaa
    24181 ttgaaggtat atcaaaatga cgaagatttc tttaatgaaa tgtttagtaa tagtccatgg
    24241 gaagcagtga gtaaaacaaa tttaggagaa tggaactttc aagatgatct agttgcattt
    24301 agcaatgatg ggtatttacg atcatattca gaaagtgagt atagagctga attaaaaacg
    24361 tatgaaacgg atattctgat gagctattta aatctattag agagtggtca gggagagtta
    24421 agagataaag ttgaagaact attaaaagaa gagctatagg gattatttca acataaacac
    24481 aagcagaaaa ctaccaaaca aaacgtacca tgcaaaaagt cggttaaacc ggctttttgc
    24541 tctattatga gaggagatca aaccatgagt gcaacaaccg atatggcttt agaagcagta
    24601 ataaaagata atacaggaaa tgtcgaaaat gtagaagact ttagcgatat tgcaatcaat
    24661 ctttcagcat ggatttcagc tgtaggagaa gaaaatatcg aatttgagat caagaatatg
    24721 gatgacttaa aagcaaaatt tccttttatc gcttcgaaac taagtcaatt tgccgaggat
    24781 aaatcgtgga atgaattagt tagtttttcc gaaaaaattt atgatattac gcaagaagat
    24841 gagtgtgttt catgggaaat gaataatcaa tacaagaaaa ttgatgcttt cgtcaaatca
    24901 cgtatagaag gaacagcttt tgagtttgaa ggtatagata ccgcagataa ttggttaaat
    24961 aaagaatcct ttattaaaag tatagcggat gatcttgaaa aacaatggtc taaggaagac
    25021 tatgcagagt acctaaaaac agaaagagat acagaggtga aagaaaaact cactgaggta
    25081 tgtttggatg aacatattga tcccaatatc gtaaaacaag aagactatga agtggttatt
    25141 gataaagttc ctactgattt tgaggaagtt gtaaagtata tagttgaaga agcattatcg
    25201 caatactctt cagatgagga agtggacgaa gaaactgttc gtaccagagt ttggcaactg
    25261 gtttttgatg acaaagatta taaatatttg agtgatcaaa actcggttaa ttggagtgta
    25321 cgattattaa ttgatcaaaa
//
LOCUS       DAZKOB010000030        24747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000030 DAZKOB010000000
VERSION     DAZKOB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.030"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            296..844
                     /gene="aac(6')"
                     /locus_tag="ACG8M6_001212"
     CDS             296..844
                     /gene="aac(6')"
                     /locus_tag="ACG8M6_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0456893.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(1092..1211)
                     /locus_tag="ACG8M6_001213"
     CDS             complement(1092..1211)
                     /locus_tag="ACG8M6_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0456894.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            1390..3051
                     /locus_tag="ACG8M6_001214"
     CDS             1390..3051
                     /locus_tag="ACG8M6_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0456895.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(3121..3468)
                     /locus_tag="ACG8M6_001215"
     CDS             complement(3121..3468)
                     /locus_tag="ACG8M6_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0456896.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            3552..4493
                     /gene="opp3b"
                     /locus_tag="ACG8M6_001216"
     CDS             3552..4493
                     /gene="opp3b"
                     /locus_tag="ACG8M6_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0456897.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            4494..5537
                     /locus_tag="ACG8M6_001217"
     CDS             4494..5537
                     /locus_tag="ACG8M6_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0456898.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            5543..6613
                     /locus_tag="ACG8M6_001218"
     CDS             5543..6613
                     /locus_tag="ACG8M6_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456899.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            6603..7556
                     /locus_tag="ACG8M6_001219"
     CDS             6603..7556
                     /locus_tag="ACG8M6_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456900.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            7568..8578
                     /locus_tag="ACG8M6_001220"
     CDS             7568..8578
                     /locus_tag="ACG8M6_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0456901.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(8863..9684)
                     /locus_tag="ACG8M6_001221"
     CDS             complement(8863..9684)
                     /locus_tag="ACG8M6_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0456902.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(9781..10059)
                     /locus_tag="ACG8M6_001222"
     CDS             complement(9781..10059)
                     /locus_tag="ACG8M6_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456903.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            10327..12336
                     /gene="metG"
                     /locus_tag="ACG8M6_001223"
     CDS             10327..12336
                     /gene="metG"
                     /locus_tag="ACG8M6_001223"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0456904.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(12815..13402)
                     /locus_tag="ACG8M6_001224"
     CDS             complement(12815..13402)
                     /locus_tag="ACG8M6_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456905.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            13577..14899
                     /locus_tag="ACG8M6_001225"
     CDS             13577..14899
                     /locus_tag="ACG8M6_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0456906.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            15054..15830
                     /locus_tag="ACG8M6_001226"
     CDS             15054..15830
                     /locus_tag="ACG8M6_001226"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0456907.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            15823..16392
                     /gene="rnmV"
                     /locus_tag="ACG8M6_001227"
     CDS             15823..16392
                     /gene="rnmV"
                     /locus_tag="ACG8M6_001227"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0456908.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            16385..17269
                     /gene="rsmA"
                     /locus_tag="ACG8M6_001228"
     CDS             16385..17269
                     /gene="rsmA"
                     /locus_tag="ACG8M6_001228"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0456909.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            17720..18349
                     /locus_tag="ACG8M6_001229"
     CDS             17720..18349
                     /locus_tag="ACG8M6_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456910.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(18473..19582)
                     /locus_tag="ACG8M6_001230"
     CDS             complement(18473..19582)
                     /locus_tag="ACG8M6_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456911.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            19791..20213
                     /gene="mgsA"
                     /locus_tag="ACG8M6_001231"
     CDS             19791..20213
                     /gene="mgsA"
                     /locus_tag="ACG8M6_001231"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0456912.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      20400..20498
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            20536..21072
                     /locus_tag="ACG8M6_001232"
     CDS             20536..21072
                     /locus_tag="ACG8M6_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0456913.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(21112..22875)
                     /locus_tag="ACG8M6_001233"
     CDS             complement(21112..22875)
                     /locus_tag="ACG8M6_001233"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456914.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(22878..24593)
                     /locus_tag="ACG8M6_001234"
     CDS             complement(22878..24593)
                     /locus_tag="ACG8M6_001234"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456915.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         8008 a         4478 c         5022 g         7239 t
ORIGIN      
        1 aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt
       61 taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg
      121 ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag
      181 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
      241 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
      301 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
      361 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
      421 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
      481 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
      541 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
      601 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
      661 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
      721 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
      781 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
      841 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
      901 aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa
      961 caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt
     1021 gaaaatggct ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga
     1081 acaccgttta ttcagataag ggactgtgac aagacttttg tcacagtttc tttttgttta
     1141 ttctttttta tctttgtatg gcaataatag acaatataac gaaaattatt taatggcttt
     1201 tggaagacca tggaaaacct tgtacaaagc tattttttct gaaaaattcc ttgaaacctc
     1261 acgaaaaaat cattatttta tgaaaaagac aaaaaaaagt gttgattttt taataattat
     1321 tcattaaact cttaaacaac ttcagaaaat attaattatt ctgaaaattc caataaaggg
     1381 gtacgtaaaa tgaaaaagaa atttacattt ggtttggttg cggtatgcgg ccttgtactt
     1441 gctggatgtt atggcggcag ttcagccaca agcagtagtt cagcaaaaag cggttcagca
     1501 gatggaggag gcgtatttaa cttagtcgtg cctcaagaaa tgcctacagc tgacttatca
     1561 gttgcaacag atacaatcag ttttacagca ttgaataacg tttatgaagg aatttaccgt
     1621 cttgatgaag acagcaaacc acagccggca ggagctagcg agttggcaga agtcagtgaa
     1681 gacggattaa cttacaaaat caaattacga gaagacgcaa aatggtcaaa tggtgatcct
     1741 gttacagcag cggattacgt ttatggctgg caacgaacag tagacccagc tacagcttct
     1801 gaatatgctt acctgtatgc gccagttgaa aatgcagaag acattacagc tggaaagaaa
     1861 gataaatcag aactagggat caaagcagtt ggtgactatg aattagaaat caaattgacg
     1921 aaacaaacac catatttcca atatctgctt gctttcccat catttttccc acaaaaacaa
     1981 tccgtagtgg aagaaaatgg ggatgcctat gcatctgcaa gtgataaagc tgtttataat
     2041 ggaccattca cattagaaga ttttgatgga ccaggaacag atacagaatg gacttataag
     2101 aaaaatgatc attactggga caaagataca gtaaaacttt ctgaaatcaa agtcagtgta
     2161 gtaaaagaat cttctactgc attgaactta ttcaaagatg gccaagcaga tgacgtgatc
     2221 ttgtctggtg agttagcaca gcaaaacgca aacgatccag cttatacttc tgttaaagaa
     2281 gcgcggacaa gctatatcga attcaatcaa cgtgaaaaga attcaccatt caacaatgtg
     2341 aacctaagaa aagctatttc ttattctatc gatcgtgaag ctttagtaaa acaagtacta
     2401 ggtgacggat cagttgtctc tacaggattg atcccatcag atatggtaaa aagtccaaca
     2461 acgaacgaag actttgcaaa agaagctgga aaattagttt cttatgacaa agataaagca
     2521 aaagaatatt gggaaaaagc gaaaaaagaa ttgggcgttg attctttaga atttgatttg
     2581 atggcttctg atgatgattc atcgaaaaaa gtaatcgaat atgttcaaaa ctcaatccaa
     2641 gaaaacttgg atggcgtaac agtcaaacca acacctgttc cattctctgt acgtcttgac
     2701 cgctcaactt cgggtgattt tgacgcagta ctaggcggat gggctgctga ctatgcagac
     2761 ccaagcagct ttactgattt gtttgtcaca ggtaattcat acaaccgtgg tcgctggagc
     2821 aatgcagaat atgacgaagc agttaaagca gcttcatcaa cagacgctgg cgatgaacaa
     2881 gcccgttggg atgatttcca aaaagcaatc aagatcatct ctgatgatat gggtgtcgtt
     2941 ccggtgtacc aaaaagcaga aggtcaccta gtgaatgaaa aaatcaaagg tatcgttcac
     3001 catgcggcag gcgcttcatg ggattacaaa tggacatacg ttgaagaata atcgttaata
     3061 ccgtgagcat gagaaaaaac gaggctgcgg caaatgtcgc aacctcgttc tcatattcac
     3121 ttatcctaac aaagaaaaaa gcatgaaaat agcagaaaga gcaattagta ttccggcaat
     3181 gatcagccaa aagctgtaca taccgtagct ggcagacgag agcgaggaaa attctggttt
     3241 tttctttcgg ttccttgttc gtgcaagatg gaccgatcgc tgaaaatgat cgaagaaacc
     3301 cgaagaaaag acccataaaa aaccgccgat aatcagaaac ggcaatgacc ataagaatag
     3361 atcattagat aattgaatga gtgtaaagga atggttgatg agatttttta ttaaaataac
     3421 cagaatcagt agaaaagcta ctgcgtaggg gatagatttt tttttcattt gattgctcct
     3481 ttatttttgg ttcattatct aataatgtag cgaaacatct gtcagaagtc aaaagctagg
     3541 gggaaacatt agtgaatagt tatatcaaat atgtattaaa acgtgtcttt ttcatgatca
     3601 tcacgctttg gttaattgca acaatcacct ttttcttgat gcaattgctg ccgggcacgc
     3661 catatacgaa tcaggaacgt ttgagtcctg aaacaatcga gatgctaaat aaacaagtcg
     3721 gtttggataa accagttatc gtacaatatg ggatttatct ttctaatttg cttcaaggag
     3781 attttggaat ttccttccaa tttaaaaacc agccagtcgc acatttgtta gctggaagaa
     3841 tcggaccatc cttgcagtta gggttacagg caattatctt tggaacagtc ctagggacga
     3901 ttttaggaac gatctctgcc atgaagcaaa atacatgggc agatacatct tctacactcg
     3961 tagcgatatt aggtcgttcc attccaaact ttgtatttgc tgttttatta caatatatct
     4021 ttgcaatcaa attacaagtt ttgccaatcg caaaatggga tggctttgtg tacacaatct
     4081 taccaacaat tgcgttggcc atgtcgccgc ttgctgactc tgctcggttt atccgtacgg
     4141 aaatggtgga agtactgcat agtgattatg tggaattagc aaaagcaaaa ggattgagcc
     4201 gttgggaaat cgcctttaaa catggtcttc gaaatagttt gattccattg atgactttat
     4261 taggaccgtt agctgtagca ttgatgaccg gatcactggt ggtagaaaat atctttgcta
     4321 ttcctggtat cggggaacaa ttcgttaagt caatcacaac aaatgattat cctacgatca
     4381 tggctgtaac gatcctttac tcatttatgc tgatttttgt tattttgatc gtggatctat
     4441 tgtacggtct agttgatcca agaattcgtg tttcagaagg gagccgagga taaatggaaa
     4501 tgataccaaa gcgttatgaa acagttgcag atatccctgc ggatgaattt cttccattac
     4561 aaaaaaatac cgaagaagaa cgtgaacaga ttactgcacc ttctttgaat tttatccaag
     4621 attcatggcg ccgtttaaag aaaaacaaag ctgccgttat ttcaatggtt ttactattgg
     4681 tcatcatttt gatctctgtg atcacaatct ttgtttctcc tcatgatcca actgcccaaa
     4741 acgtggctta tatcaatttg cctcctcgta ttccgggaat caatatcgat ggattgaatg
     4801 gaaaagcaat ggttggtggt gaacttgttg ataaatatgc tcaagcaaac gtcccttcaa
     4861 acgtaaattt ctttttagga acagacggtt tgggacgtga tgtattaagc cgcttattta
     4921 tgggaatgag aatctcgttg ctgatcgcat ttattgctgc attactagat gtaacgattg
     4981 gtgtagctta cgggttggtt tctggtttgt taggcggacg tgtcgataac gtgatgcaac
     5041 gttttttgga aatcttatca ggaattccaa acctcgttgt catgattttg atgttggtcg
     5101 tttttgaacc aggaattttt tcaatcgtgg ctgctatggc catcacaaac tggataccaa
     5161 tggcacgtat cgttcgtgca caaacaatga aattaaaaga tcaagaatat gttttagctg
     5221 gattgacatt aggtgaatca agaatgaaaa ttgcattcaa acatattttg ccgaatatct
     5281 ctagtgtcat tattattcaa atgatgttta gtattccatc tgctattttc ttcgaagcct
     5341 ttttaagctt tatcggttta ggtcttacgc ctccgtcagc ttcattagga acgatgttga
     5401 gcgaagggta caagacattc ctgtatctgc cttatctatt atggattccg gcagtaactt
     5461 tatcggttat catgattggc tttaacttac ttgctgatgg attgcgtgat gcctttgatc
     5521 ctaaaatgaa agagtgaaga cgatgacgaa aattttagaa gtgaaagact tagaaatctc
     5581 ttttgatacg tatgctggaa aggtacgagc gattcgcggt gtcaattttc atttgaataa
     5641 aggagaaaca ttggcgatcg ttggggaatc tggaagtgga aaatcagtga cgaccagaag
     5701 catcatgcgc ttgctgtcaa gcaacgccaa tatagatgct ggtcaaatcc tctttaaagg
     5761 taacgatatc gttggaaaat cagaaagaga gatgcaaaaa atccgtggta aagaaatagc
     5821 gatgattttc caagatccaa tgacctcttt agatccgacg atgcccattg ggaagcaagt
     5881 cgctgagtcc cttagaaagc ataataaagt ttcaaaaaaa gagggacaaa aagctgcatt
     5941 agaattgctt aaattagttg gaatacccga agctgagaaa cgtatcaaca gttatccgca
     6001 tcagttctca ggaggtcagc gtcaacggat cgtgatcgcg attgcactgg tatgttatcc
     6061 ggaaatactg attgctgatg aacctacaac agcgcttgat gtgactattc aagcacagat
     6121 tttggagctg ctgaaagaaa ttcagacaaa aatcgataca tcaattatct ttatcactca
     6181 tgaccttggt gtagtagcga atgtcgctga tcgtgtcgct gtcatgtatg gaggaagaat
     6241 cgttgaagtt ggaacatcgg aagaaatttt ctacaacccg caacatccct atacttgggg
     6301 actgcttggt tcaatgccga cattagatag tgcgaacgat cgattgtatg ctattcctgg
     6361 ttctccaccg gatttattga acccgccaaa aggagacgca ttctatccaa gaaatgaatt
     6421 cgcgatgaaa atcgatgcag aacaagaacc accatttttt gaactatcaa aaacacacaa
     6481 ggcagcaact tggctacttg ctccgcaggc acccaaagtg acaccgccag aagaaatcca
     6541 acgtcgctgg gctatcttta aagagaaaca aaaacatact tacggtcaag gaggaattgc
     6601 tgatgccaaa taaactactt gaagttaaag gattgaaaca gtatttcaat gtaggaaaaa
     6661 agaatgaagt acatgctgta aatgacatca gctttcacat ttatgaagga gaaacattcg
     6721 gtttagttgg agaatctgga agcggaaaat ccactactgg ccgtacgatc attcgcttga
     6781 atgaacctac tgatggtgag atattattcg acggtcaaga tataacgaag atcaaagata
     6841 aaaaaggttt gacgaaattc cggcatgatg ttcagatgat ctttcaagat ccatacgctt
     6901 cgttgaatcc tcgtatgaaa gtcagagata tcattgccga aggaattgat gttaatggac
     6961 tagctaaatc atctgaagag cgtgcaaaaa aagtcgatga tctattgaga actgttggat
     7021 tgaatccaag ccacggtact cgttatcctc atgaattttc aggtgggcaa cgccagcgta
     7081 ttggtattgc acgagcattg gcagtaaaac cgcgttttat catttgtgat gaaccaattt
     7141 ctgcactaga tgtgtcgatc caagcccaag tggtcaattt gttgcaagat ctccaaaaag
     7201 aacatcaatt gacctatcta tttattgcac atgatttgtc gatggttaaa catattagtg
     7261 accgaatcgg tgtaatgcat aacggcttgt tattagagat gggaacaagt gatgaaatct
     7321 ataatcacgg tgttcatcct tatacagaaa gtttgttatc tgctattcct ctaccagatc
     7381 cagaccatga gagaaaacgc agaagaatca aatatcaacc tgaaccagat gacggacaag
     7441 tacgtcaact tcgtgaaatc gcaccagaac attttgtcta tgcaactgag caggaagtcc
     7501 catattacgc aaaaaaacta aaacgacaaa aggaatctct tctggttgct aactaggagg
     7561 gacatagatg ctaaaagctt ttaaattccg tatgtatccg acagaagaac aaaaacagca
     7621 attgattcgt acttttggat gtgctcgatt tacctataac catttgctga aaaagcggca
     7681 gaaaagttgg caacaaacag gagtagccaa tttttcgctc acacccgcta cattaaaaaa
     7741 agaataccca ttcttaaaag aagtagacag tcttgcttta gcaaatgcac agcttaattt
     7801 ggatcgggct tttcgcaatt atttcaaggg acgtgccagc ttcccaaagc ttaaaacgaa
     7861 aaaaagtatg tggcaatctt atacgaccaa caaccaaacc cgtacgatct atctggaaaa
     7921 tggctactta aagttaccaa aacaaaaaga actaatcaag ataaatcagc atcggccagt
     7981 tgaaggatcg attcgctccg ctacgatttc tgctcgatat aatgaagaat tttatgtcgc
     8041 actactttgt gatgtttcgc cagtaaaaaa agaatcttta gctaaatgga tcgggatcgc
     8101 ctatcatccg aagacattga tccaaacatc tcgacctcta gaagtaactt taccaaaatt
     8161 tcaccagaca gaagaaaagc tgcaacatgc acagcggaaa ttgaatgtga aagtccgctc
     8221 cgctcaccat agaaagatca ggttagatca ggcaagcaat tatcagaaac aaaagagaaa
     8281 agtaatggat ctttatctga aacaaaagaa ccagcgagaa gattatcttg aacaattatc
     8341 tgggaaattg gtcaaacaat atgactactt atttgtagaa tcttttccaa aagaagaggc
     8401 acatgctgat ttttctattc acgactggca taaattaata acaaaattgc gttataagtc
     8461 ccaatggtat aataaaaaat ttcttttgat caataccgac ggagcagaag aaagcaattc
     8521 cgtaagaaag agccaagtag tagaaaaaat gggcagacat tcggttatca agggataaaa
     8581 aatgaatgaa actgatagga gcgttagggt tagccttgtt aataaaaagt cttaatgact
     8641 tttgtttcta ggaatttttc ttttgaagaa aagctcacta attatgacaa accaataaaa
     8701 caatagcaga aaaagttcgt acgagctttt tctgctattg ttaatttttt attcagaaag
     8761 tttacgaata catcaaaatt ctgaaacaaa aaacgtaact cacaaagagt tgtttttgcg
     8821 ttttttcttg aatgattagg tctgaatctt ttgctcacaa ccttattcat ggtgccccgg
     8881 cttgagctga tagatttttg aaggacggcc gattcctaca ggacgttcac cgatctcatc
     8941 gaaatattgg agcatagctt tcttaaaatt ggaatgatcg atcgttttat aatcgatccc
     9001 taagaattta gcaaatactt ttcgagcttc cgtaatcgtg aaatctttac ctaaaacttg
     9061 tagaacttgc ggctcgtgtt ccattttatt gactacacgg ttgaatgctt tcaagatgat
     9121 ttgactatgg tcaaaagcca aagtatcttt gccagaagac tctccagtga ctaaatcaag
     9181 cgagatagcc acttctccgc tagataaatg gatcttgtta ctatgacgtt ctagtgtgaa
     9241 ccagcgtact tccttggcat catctccggc gattagcggt tcttctccaa taaaggctaa
     9301 gtagctgact gtgacgaccc agcctcgcgg atctcgatta ggcgtactga atgtatgcag
     9361 ttgctcaata ttttcttttg aaataaccac accagtttct tcctttgttt ctcttaagac
     9421 gctttctcca gttgactcat ccttttggac aaagccacca ggcaatgccc atgaatttcg
     9481 ataaggatgc ccttttcttt ggatcaacaa aactttcagc tgatcttctt ctttgttata
     9541 gcacatcagt acgatatcga cagtcaaaga gggcttgtca tattctggac gttcctgttt
     9601 ttgataccag ttaagaaatt cttcttcact tgctttatgt tcataatata atttttcttc
     9661 ttctttagaa gcaaaacggt tcatctgacc acctccattg acagtaaaag tactttttac
     9721 cttattgtag cagactctga aaaaaaacca aggatttact tgaaaaatct aggttatcat
     9781 ttaaatatga aaaataaatg gaagtatgag attgaataaa aagaaaagca gataaaacgg
     9841 caatacttta taccgctctt cacgaatggc aaagaagcca caaataacga tatatacgtc
     9901 aatgataaaa cgcaaaatcc aagctgctgt cataaaactg ttcaaatgcc catggaggtt
     9961 aaaataacca tacatacgaa tataaaaata gctgaatatt aaatgtgcta atgaaagaaa
    10021 agcaatacta agtgtaaaaa aaagatgttt cttctgcatg attgttccag tcccttacta
    10081 agatagatag tctttattat accacgtgtg aggggagaaa taaaaagccg ttttttagta
    10141 gatatgaaac aaaacaaaaa cccgcattta ttcaacctat tcatcctttt aaaggataga
    10201 ttgaacgtag atagaagtcg tttatccagt ctttttcatt ttgaaaaaaa gctaacagca
    10261 tgcttttttt atggtatact agcaaggaaa cgaattcgca tcatgcgata aaggaggaag
    10321 agccccgtgg cagaaaagga aactttttat atcactacgc caatctatta tccaagcggc
    10381 aaacttcata tcggcaactc gtatactacg attgcatgtg acgcaattgc tcgttataaa
    10441 cggttgatgg gctttgatgt gttttatctc actggagtgg atgaacatgg tcaaaaaatc
    10501 gaaacaaaag cagaagaact aggtgtaaaa ccgcaagaat atgtagataa aatggcggct
    10561 gatgtcaaga aattatggaa aacgcttgat atcagttatg ataaattcat tcgcacgaca
    10621 gatgattacc ataaagcagc tgttcaaaaa atatttgatc gtttattaga acagggagat
    10681 atttacttag gtgaatatga aggctggtac tctgtttctg acgaagaatt ctttacagaa
    10741 acacaacttg cagaagtgta caaagatgac gaaggtaacg tgatcggtgg taaagcgcca
    10801 agtggacacg aagtagaatt ggtaaaagaa gaatcatact tcttccgcat gagcaaatac
    10861 gcagaccgtc tattggaata ctatgaagaa catcccgaat tcatccagcc agaatctcgt
    10921 aaaaacgaaa tgatcaacaa ctttatcaaa ccaggattag aggatcttgc agtttcacga
    10981 acaactttta cttggggagt accagtcaag agtgatccga aacatgtggt gtatgtatgg
    11041 atcgatgcat tatccaatta tattactgca ctaggatatg gctccgaaga tgaaagtcta
    11101 ttcgaaaaat actggcctgc agatgttcag atggtcggta aagaaatcgt tcgctttcat
    11161 acgatctatt ggccaatcat gctgatggcc cttgatttgc ctctacctaa aaaagtattt
    11221 gggcatggct ggctattgat gaaagacgga aaaatgtcta aatcaaaagg aaatgtcgtt
    11281 tatcctgaaa tgcttgtgga acgttatggt ttagatgcac tccgttatta tttgcttcgt
    11341 gctgttccgt ttgggtcaga tggtgtcttt acgccagaag attttgtttc ccgtgtcaat
    11401 tatgatctgg caaatgatct agggaactta ttgaatcgaa cgattgctat gatcaacaaa
    11461 tattgtgatg gactcgttcc tgactatgcc tcacttgtca ctccatttga tagtgaactg
    11521 tctacgacgg ctgctaacgt tgtcggacgt taccatgatg caatggaaaa aatggaattc
    11581 aatacagcat tggctgaaat ctgggtgctg atttctcgtg caaacaaata cattgatgaa
    11641 acagaaccat gggtattagc aaaagacgaa gaaaagaaag ctgaattgaa tagtgtcatg
    11701 atccatttag ctgaatcttt gcgtatcgca gcgatattac ttcagccaat catgacagaa
    11761 acgccagaga agatcttcaa tcaactagga ttggactctg aaacaatgaa tcttgaagga
    11821 ttacactttg gcgaatttcc ttcaggaaca aaagtagttg caaaaggtac accaatcttc
    11881 ccacgtttag atatggaaga agaagttgct tttattcaag aaaaaatgtc tgaaggtact
    11941 caaacaaacg aagatacagt caaatgggat ccagaagaaa cagaacttgt ttcaacgaaa
    12001 gaaaaacaaa tcaaatttga tgtatttgaa aaagtagaac taaaagtagc agaagtcatc
    12061 aactgtcaaa aagtagaagg ggcagataag ttgctgcaat tccgactaga tgctggagac
    12121 agccaagacc gtcaaatctt gtcaggaatc gctgaatttt atcctgatcc aagcgaattg
    12181 atcgggaaaa aagtcgtcat tgtagcaaat ctgaaaccac gaaagatgcg tggacaaatc
    12241 agtcaaggaa tgatcctttc tgcagaagca ccagatggct ctttacaagt aatcgaagca
    12301 ccaaaatcca tgccaaatgg ctcagaaatc gcatagaaat tcaaaggggg tatgacacta
    12361 gttgtgtcat accccctttt tccgaaattt gagaagcaat tttcgtaaat tccttcttat
    12421 tacttgaagc caatcatata atacagaaac agagaactat ccaaaataaa tcctagaaaa
    12481 agaagtacga gcttcgctga tttttgttaa tttagaataa tactaatagg aatcgtaaaa
    12541 acagaaaaaa agcataatga taaagggtcg ggcagatttt aattgattca tctggtactc
    12601 ttttttattg tccttacatt cataaagttt gaatgcaatg actttattac atctatattt
    12661 atatctaaca gtagcgttga tgtgttaatt ataagttcta tcactttatg ttcaattagt
    12721 ttgtgctctt atatgtgtga ttgaaaatat agataaaaaa acaatcatcg gctttgactt
    12781 cgatgattgt tttctttatt ttgtaaaaat cctatcactt gattttatgg taacctttta
    12841 tttttgtctt caaacggctt ggtaagagag cagtaagcag tgcaaatggg atcgaaaaga
    12901 cataccagaa aatcgtacgt ccgataccat aatgttgata gagctttgtc aacatcacgt
    12961 gtaacaagat caaccctaaa agcaaggagc cagctataaa ccaatgatta tcccaatgca
    13021 tgacttcact aagtgccatc actccaaaag gatagaaata aaaatagatc ccgactaagg
    13081 tcatccgcca gttctttgta aatgttccga tattcaataa aaagaaaatc gctgaaagca
    13141 aaataccgat aggcggaaaa agcccaacca aaaaggaata caacatcccc cacaagatca
    13201 tcacaggacc tttgatcaat gcaacgattg cgatgaacaa agtcagcaat attagttttt
    13261 gtgataacgt tgcaacagtc attaatccaa ataaaattaa tgtgaaccaa tgccaatggg
    13321 actgctgttg ttctttacgg gaaaaatcga cccggttgga taaataatct gctaatttcg
    13381 gataagaacg cttaaaatcc ataaactgtt cctccttcgt ggaaaaggta ggtttagtat
    13441 acgaaaaata aaaaagaaat acatccgtct taagagggag tttttcactg aaagtggaga
    13501 aaaaagaatg gtaaattaaa atattctcag ttatactgaa aattatacca aatttcaaag
    13561 aagaaagagg atgggaatga aaggcaaact aaaacgagaa atcaatctgt ttggcgcatt
    13621 agctaccgtt atgggaaccg tgatcggtgc aggcgtcttc ttcaaaactg ctgctgttac
    13681 tgctagtact cagtctacaa gtttgacatt gctagcttgg cttttgggtg gtttcttaac
    13741 gatctgtgct ggattgacag tggctgaatt agctacagca atcccggaaa ctggtggagc
    13801 tgtaaaatat attgaagctg cgtatggaaa attgcccagc tttttattag gatgggcgca
    13861 aagcttgatt tactttcctg ctaatattgc tgcattatcg attatctttg cgacacaatt
    13921 gacgaactta ttacaattat ctaccgatta tcttttgttg attgcagtga tcaccgctgt
    13981 ttctgtgaca gggttgaatt tgttagggac aaaagtcggt gcttctgtac aatcagtgac
    14041 gttgattgtt aaattaatac caattgctgt gattgtcata tggggattat tgaccccagg
    14101 acaaggaact gtccagttat ttccaatcga agctggaaaa gatgttactt ttgtcgaagg
    14161 attgagcagt gcgttgttag caacgttgtt tgcttatgac ggctggttag gtgtcggtgc
    14221 aatggcaggg gaaatgaaac ggccggaaaa agatttgcct aaagcaatta ttcttggttt
    14281 gagttttgta acagtcgttt atctattgat caattttgtt tttttgaaaa ctttgccgat
    14341 tgatcatctt gccggtaatt tgaatgcagc ttctgaagca tcagacgtca tttttggagg
    14401 aatcggtgga aagctggtca ctattgggat cttgatctcg gtatacggtg cattgaacgg
    14461 ttatacacta actgggatcc gcgtgcctta tgcgatggct ttagaagacg agctaccttt
    14521 cagtaaacag ctgacaaagc tttcaaagaa gtttacggtt ccttatgttc ctgcggtttt
    14581 ccagttagca gtagcttgta ttatgatgag ccttggttct ttcgactttt taacagacat
    14641 gttgattttt gtgatgtggc tattcagttt actgattttt atcggtgtct ttattttaag
    14701 aaaaaaagcg ccggaattgc cacgtccata taaagtacca ctttatccaa tcgttccgat
    14761 tattgcgatt ctcggtgcta ttttcatttt aggtatgacg atgatgacgc aaacgaaatt
    14821 agcattgatc ggtataggtg ttaccttgat aggtatccca gtctattatc aaaaaaagaa
    14881 aaaacgctca gaagagtaaa atatttcaac aacagacaga aaaagtacga actaggtcct
    14941 ctagatcttc gtgctttttc tatttcaata aaacaagttc ttctattttg atttttgcta
    15001 cttctgcaag tagtgcttat gtgctaaagt aagaatgaaa aggagagata aacatgatat
    15061 ttgattctca tacccattta aatgctgaac agttcaatga agatattcca gaaacgattc
    15121 agcgagcaca agaaatgggg gtaacgaaaa tggcggtcgt gggcttcgat acgccaacga
    15181 ttgaaaagtc tttgcaatta agccatgact atccaaatat atacagtatt atcggctggc
    15241 atccgacaga ggctggcagt tatacgaaag aaatcgaaaa taaattacaa gaacaattga
    15301 ctttaccaaa ggttgtagca ttaggggaaa ttggcttgga ttatcattgg atggaagacc
    15361 cgaaagaagt ccaagataaa gtatttcgca gacagattgc tatcgcaaaa gaaatgaacc
    15421 taccaatcag tattcatact agagaagcac ttgaagacac gtatcaaatc ctaaaagaag
    15481 aagatgttcg agatatcggt ggaatcatgc atagtttcag tggtgattat gaatgggcaa
    15541 aacgatttct tgacttaggg atgcatattt catttagcgg tgtcgtcact ttcaaaaaag
    15601 cattagacgt ccaagaggct gcagcaaatg taccaatcga acgattactt gtggaaacag
    15661 atgcccctta tctggcacca gttcctttta gaggaaagcg aaatgaacca ggctatactc
    15721 gttatacagt agaaaagatt gctgaacttc ggaatcttcc gacagaagaa gtggctgccc
    15781 aaacatggac aaatgcgcac cgattatttg gtctaagcga acatggataa gatgaagatc
    15841 caagagatta tagtcgttga aggaaaagac gatacaagaa ggctgcggga agtagttgat
    15901 gcagatacga tagaaacaat agggtctgca atcaatgaag agatcctgat gcaaattgaa
    15961 catgcccaag agactcgtgg agtgatcgtt tttaccgatc ctgatttttc aggagaaaaa
    16021 ataagaaaaa tcattatgga agctgttcct caggcaaaac atgctttttt gccgcgcaca
    16081 caagctaaac caaagaaaaa gggaagcctt ggtgtcgagc atgcaagtga tgctgctatt
    16141 ttagaagcat tgaaaaaggt cgtgacgcca gacatttccc aaacggctgt ttctgaaatc
    16201 actagacaag accttttaga ctatggttta atagctgggg cacaagcaaa agaaaaacga
    16261 gaaaaactag gtgacgagtt gcgcatcggt tacacgaacg gcaaacaatt ggaaaaacgt
    16321 ttgaagatgt ttcgtattac gccgaaagaa ctcgcccaag caatgaaaat agtggaggaa
    16381 caacatgagt gaatatcgtg aaattgcgac accctctaga acaaaagaga ttttattgaa
    16441 acatggtttt tcatttaaaa aaagtttagg acaaaatttt ctgactgaac caaatatttt
    16501 aaggaaaatc gtagaaactg cgcaaataga tgaacatacg aacgtgatcg aagttggacc
    16561 aggaatcggt gcattgacgg aacaattagc caagcatgca aaacaagtcg tagcatttga
    16621 gatcgatgat cgtttgatcc ctgtattggc tgatacgatg caaccctatg acaatgtgac
    16681 tattgtacat caagatattt taaagaccga tttgtcaaca gctgttagag aaacatttca
    16741 tgaggagctt ccgttaaaag tcgtagcgaa tttaccttac tatattacga caccgatcat
    16801 gatgcatttt cttgaatcag atttggttgt ggatgaacta gtcgtcatga tgcaaaaaga
    16861 agtagctgat cgtatctctg ctgaaccagg aacgaaagct tatggttcat tatcgattgc
    16921 tgttcaatat tatatggaag ccagtttagc attcatcgta ccaaaaacag tatttgtccc
    16981 tcagccaaat gtagattcag cgattttgaa actgacaaga agagatatac cggcagtcga
    17041 agtgacagac gaaaaagcat ttttccgatt gacaaaagcg gcatttcagc aaagaagaaa
    17101 aacgctttgg aataacttgc agcattcgta tggcaaagat gaccagacaa aagcatggtt
    17161 agcaaaaagc ttggaaacag ctggaatcga tccaaaacgt cgtggggaga ctttgtcatt
    17221 acaagaattc gccgcattaa gcaatgcgat gtcagaaaat atgcagtaaa aaaagaggtt
    17281 gtgacagaag ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
    17341 agttcctcat gtttttcgtt tgtttgggct tattaccgca gatgcaagct tttgaacacc
    17401 gtttaagaaa gaggttgtgg gcctgccgtt taactctaag tcacaaggaa caatttcaaa
    17461 aaacagcttc tcatgttttt gagttagttg gtcttattac ttgatgcaag cttttgacca
    17521 ctattatgcc gaacaaggag atgtgacaca aattttgtca catctcctat ttttgtttct
    17581 tgatcaaaaa attcttatac ttgatactca cactcatttt gtatggattt agaaaagaat
    17641 atgtgtcttg acagttatta gtaagtgagt tatactaagt cgacatttat gtgtcaagac
    17701 atattaggag agagaaaaaa tgagaaaaaa ttcagttcga aaactaacga tttctgcttt
    17761 attgattggg atgggggtca ttatcccaat ggtcatgcct aaaatcatga ttccaccagc
    17821 ttcctttact ttggcgtctc atgtgccatt atttattgcg atgtttttta caccaggagt
    17881 tgcagtggct gttgcgctag gtacgacatt tggtttcttt ttaacaacgc cggttatcat
    17941 cgcattgcga gcattgtcac atcttgtgtt tgcgttgatt ggggcttggt acttgcaaaa
    18001 gcatccgagt atcgttttaa aaaatggaca gtttactttt gctaattggc gtttccaagg
    18061 gttcaatttt gtgatcggtg tgattcattc tttagcagaa atgctagtag tcagcatctt
    18121 ttactttatt ggtaatatgc ctgaaacata ttattctcaa ggatattttt atacagtatt
    18181 catcttgatg gggattggtg ggcttatcca cagtttagtt gattataata ttgcttattt
    18241 tatagcagga acactcagca aatattttga tatccccgta tttacggcag caaaaaaaga
    18301 aaatcggaaa gagtcgattc aagggtacgc gaagaaaacg acatattaga catgatctct
    18361 caaaaaaaaa actctcatct agtaatggat aataaatcct ctaagatgag agttttttgt
    18421 cctaaaaaga gctgcaccaa catcttctgg cacagctcta acagggggca atttatggaa
    18481 gtttgatgac ttgttctgtt tctttatcaa agaagtggcc tttgctgatg ttgaaggcaa
    18541 ggtcgactat ttcgcctggt ttatggaaat ctcttgcatc tacttttgag atgaactcag
    18601 tagaaccagt ttttgtgtaa agcatcgttt cagctccaag taattcggaa acgacaactt
    18661 ctgatttcac tacggcttca ggtgaagctt caagggctac ttgttcactg tggatgtctt
    18721 ctggacgaat accgaagata acttcttttc cttcataacc tttttcaact aatactttgt
    18781 tttttccttc aggaatacga agcttaagcc catgtccatc tgtgataaca ccatttttca
    18841 atgtcacatt aaagaagttc atggcaggag atccaataaa gccggcaacg aacatattga
    18901 cgggtgtatc ataaacttct ttcggtgtac cgatttgttg gataaatccg tctttcatga
    18961 taacgattcg gtcagccata gtcattgctt ctgtctgatc gtgagtaaca tagatggtcg
    19021 ttgtttcaag acgctggtga agtttggcaa tctcagcacg catggctaca cgtaatttag
    19081 catctaaatt tgataatggc tcatccatta agaagacttt tgcatcccga acgattgccc
    19141 gtcccaatgc cacacgttgg cgttgtccac cagataaagc agctggtttt cgtttcaagt
    19201 attctgtcag acctaagatc tctgctgcat tgtccacacg ttttttgatg tctgctttat
    19261 catatttacg taatttcaag ccaaaagcca tattatcaaa aacagtcata tgaggataca
    19321 atgcgtagtt ttggaaaacc atggcaatat cacggtcttt aggtgccacg tcattcatga
    19381 cttttccgcc gatatttagt tctccttcag agatatcttc cagtcccgcg atcattcgca
    19441 aagtagttga ctttccacaa ccggaaggac caacaaaaac gataaattca cggtctttga
    19501 ttgatagatt aaaatcagtt acagaataat tttctgcatt gtcatatttt ttatatacgt
    19561 ttttcagagc catttctacc ataatacgtt ccacactttc ttttttagtt ttattaacta
    19621 tttaaagtat aaatgaaagc gttaactaac ttcaatgtca tgttgaacaa gaattttttc
    19681 ttttttttgt caatgtgcta gttttaagct ttaataaata tccataagaa ataaaaactg
    19741 atacactgga gtaaataagt atgttaacaa tatcaaaagg agtgtctctt atgaaaatcg
    19801 ctttgatcgc tcatgaccga aaaaaaccta tgatggtgaa attagcaaca gcgtataaaa
    19861 caatattgga aaaacatgaa ctttttgcta ccgggacgac tgggcagagg atcacggaag
    19921 caaccggctt gccgatccat cggtttaaat ccggaccgtt aggcggggat cagcaaattg
    19981 gagccatgat ttcggaggat cagttagatt tggtgatttt tcttcgagac ccgctggctg
    20041 cacagcccca tgaaccagat gtaacagcac ttatccgttt atctgatgtc tatgagattc
    20101 cgttagctac gaatatcgga acagctgagg tgctactaag aggactggaa gccggcttcc
    20161 tagattttcg aagaatcgtc catgaaatag acaagcatcc actttcattt tgatggataa
    20221 gcgtatggaa aaataacgtt taatgttcgc ctatatgtag ctataaaagc taaacaaact
    20281 gataatatgc gatataatac gaacgtattg gataatataa acaataatat tagtttttcg
    20341 tatttgcagt aaatgtgaaa atatgctata gtttctgtgg gaaaagatcc caaaaataat
    20401 acagagtaga aaataaagcg ttaagtgcta agaggatggg atgttgcctc ttggacgaag
    20461 gacgtaatgt tcgcggtttt attttcgcat tcgctgcatg atacgatgta gcagctctta
    20521 aaggagatgc taaaaatgaa gaaacaccgg ttagacgcac gtatgattgc tattatgggc
    20581 ttattgattg cattgatggt cactttgtcc cgactggtag caatcgagac cccatttatt
    20641 aaaatcagtg tcacatttat tccacaagtg atcatgggca ttctttttgg tcctttttgg
    20701 tcagggatag gcgcagtttt agctgattta gtaggaatgg ccctcttctc gaaatctgca
    20761 ttctttattg gatttacatt gaatgcattt attgaaggag caatctacgg attctttttc
    20821 tatcgaaaag agatcacttg gaaaaatgcc attctggcta cgcttagtgt cacgctgatc
    20881 atcaacttat ttttgacacc attatggctt gcgttgatgt accatgtgcc attgttcagc
    20941 tgggtcgttt gggcgccccg cctgttaaaa acagtaatat ggttaccgat tcaatcaatt
    21001 gccatttatt acgtaggacg tagtattcct tacaaaaaaa ttttaagaag tttagcgatc
    21061 cacgctaaat aaaaaacaag cagtcttccc atcccagaag actgcttgtt tttattgtgc
    21121 tgtgaattgt gcttgataga gccgtcggta ataaccatct ttttgaagca gctcatcatg
    21181 ggtgcccatt tcgacaatag ctccctgatc catcactaat atctggtccg catttttgat
    21241 tgtggacagg cgatgggcaa tcacaaaact ggtttttcct tccatcattg ctaagaaggc
    21301 ttgttggatt tttttctctg tcaatgtatc gactgagctg gtagcttcat ctaagatcaa
    21361 cataggggga ttactgatca tcgttcgagc aatcgtcaac agttggcgtt gtccatctga
    21421 tatctttaac ccttgaccgc caatcttagt atctaatttt tccggcaaac gttctacaaa
    21481 ttcatacata taagaggctt ttagggcatc attgatttct gcgtcacttg cttccggatt
    21541 cccaaaacga agattttctc gcaatgtact gtcaaatagc caagtatctt gtaacaccat
    21601 tccaaaactt ttacgtaatg tatcacgttg gatagagcgg atgtcatggt catcgactgt
    21661 aattgttcct tttgttgtgt cataaaaacg catcagcaaa ttaacaagtg tggattttcc
    21721 ggcccctgtt tttcctacta tagcaatggt ttgacctgga taagcagtaa aattgaaatc
    21781 ttcgatcaaa cgctgcttgg ggtcataaga aaaagaaaca tgatcaaaag agacttttcc
    21841 tttgatattt ttgggttcca aggcataggc atctggcaaa tcaggctttt cttctggctg
    21901 atcgatcaaa tcaaatgctc gttctagacc agaaaaagcg gtttgcagtt gtgtcgtgat
    21961 tccagaaagt tcgataaatg gtttagaaaa ctgacttgag tagatcgtaa agctggaaat
    22021 cacaccaaca gtgattgacg aaccactctt caatgccaat aggccgccaa ctaaaccaat
    22081 cgataaataa gcaaggtgat ctacaaaacg agaagacggg tttgtcaagg aagaagaaaa
    22141 ttgtgcccgt tgtccttttt catatagatc agcatttagc agttcaaatt gctgttgact
    22201 agcttcttct tgctgaaaag ctttcacgat tttctggtta ccgattcgtt cggtcacaaa
    22261 gcccgagata tccccaataa tcctttgctg tctatcgaaa tttgtttgtg aagcacgagc
    22321 aacgatccaa ctcacaagga aaataattgg tgtagaacaa agaacgacca atgtcagaag
    22381 tggacttaga cgcatcataa aaataaacga aagaatgatg attgccactc cagaaaataa
    22441 ttgattgaag gccgcagaaa ttgcaatgga aatattgtcc atatcatttg taaatcgact
    22501 gacgatattt ccgtgggagt tttgatcata ataacgaagt ggcaatcgat tcagatgagc
    22561 aaatgcatct tttcttaatt ttgcaacgga taagtaagct aaccgattgc ttaacacttg
    22621 gatgagccat tgactgacta cgttgattaa aacgatacca ccgaatagaa taagtaaatg
    22681 gaacagtcga ttgaagcgga cctgtccttc tccaatcatt tgatcgatac tttgaccaat
    22741 ttgtaacgtc atcacaactg ttgcaatccc attgatgatt cctagtatca cagctagcca
    22801 aatgtctttt gcataggcag taaggtaagg aatgaatcgg cggagcgtct gcaaggataa
    22861 tttttttact ttcataacta ttcttcctcc tgtgatgcga cgatttcctg atattctgct
    22921 gattgagcaa gtaattcttc atgggtaccc tgagcagcaa tcttaccttg atccatcact
    22981 aagatatgat gtgcttcttg aatagagcgg acacgttggg agataataat cactgtactg
    23041 ccaagataag tctgtagcgc cttacgcaaa tccaagtctg tttgataatc caacgcactt
    23101 aaagagtcat ctaaaatcag taatgctggc ttggcaatca aagctcttgc aatcgtcagt
    23161 cgctgttttt gtccgccaga gaaatttttc ccattttcca tgacttttgt atctaaacca
    23221 tttggaagct gacggacaaa ttcttctgcc tgagcgattt tcagagcttc ccaacattct
    23281 ttttctgtgg cgtctttctt accccattga aggttttcac gaatagtccc ggtaaatagt
    23341 acagaagttt ggggaacaag agcaatttgc tgacggagat gatccagctg ccaagaacga
    23401 acatccattc cgtccattat aactgtaccg gaactgacat catagaaacg cggaatcaat
    23461 tgtgtcaaag tcgttttccc gcttccggtt ggaccaatga ttccaaggac tgtatttcgc
    23521 ttcagctgaa atgtaatatt ttggagagaa agaccagaat caggagtata acggaagtca
    23581 acatggtcaa aagcgataat tgcttcagaa gaaaaatcgg gagtagtact gacctcagct
    23641 tcaagaatcg aattttctgt gtctagcacc tctttcaccc gatttcctga agcttcggca
    23701 cgtgtgaaga tcacgacgag gtttgaaaca acgatcagtg ccaaaagcat ttgattcata
    23761 taattgatca aagcaagaac ttgtccttgt tgcaagctac caatatttac tttgatcccg
    23821 ccaactgtca gcaaacagat gatcccgaca ttcatgatca aggttgtagc aggtgataat
    23881 agggcagaga tattggaaac acgtaaataa ttgttcgcta gatcatttgt gcttttatcg
    23941 aacttctctg tttctttttt tgtctgcgca aatgcgcgga tgacacgaac accgctaaga
    24001 ttctgactaa cgtgttcatt gagctgatct agtttttctt gcactttttg gtagagagga
    24061 acggtttttt tgatgataaa aaataaaatg attgagaaaa taggcaataa tgctaagaaa
    24121 aacagaccta cttcccagtc gatcacaaaa gccatgacca ctgacccgat acttaaaaac
    24181 ggtgctctga tcaataaacg aatgaccatt gctaaagcta gctgaagctg attgatgtcg
    24241 tttgtcattc gggtgatcaa ggtatctgtt ccaaaatgat tcaactcgtt gtgagagaaa
    24301 gtattgattt ttttgattag ctgattacgt aactctgtac caaatccttg tgaagctact
    24361 gaagcatagt attggcaaat gatagcacaa atcagaccaa tcacagacat agcgaccatc
    24421 cataagccca tttcaatgat ttttcctcgg tcattcattt ttagtccatt atcgatcaag
    24481 gaagccatca ttaaaggcag gacaagttcg aagacagctt caagaaattt gaagaacggg
    24541 cccaaaatga tttgcttacg atagtctttt gcataacgta ataactgaat catatgattc
    24601 ctcctattat ttatttgtcc acaattgaaa aaacaagtac taccacattg tagcttagga
    24661 aatttagaat gaaaagagaa caagcctttt cataaatgaa agttttatta tgaagaagct
    24721 ttagaaattg aatagactat ttttttt
//
LOCUS       DAZKOB010000031        24338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000031 DAZKOB010000000
VERSION     DAZKOB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.031"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..2478)
                     /locus_tag="ACG8M6_001235"
     CDS             complement(133..2478)
                     /locus_tag="ACG8M6_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0456916.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2757..3659
                     /locus_tag="ACG8M6_001236"
     CDS             2757..3659
                     /locus_tag="ACG8M6_001236"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0456917.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3870..5645
                     /locus_tag="ACG8M6_001237"
     CDS             3870..5645
                     /locus_tag="ACG8M6_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0456918.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5711..7297)
                     /locus_tag="ACG8M6_001238"
     CDS             complement(5711..7297)
                     /locus_tag="ACG8M6_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0456919.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7548..7772
                     /locus_tag="ACG8M6_001239"
     CDS             7548..7772
                     /locus_tag="ACG8M6_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0456920.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7914..9665
                     /locus_tag="ACG8M6_001240"
     CDS             7914..9665
                     /locus_tag="ACG8M6_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0456921.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9665..11449
                     /locus_tag="ACG8M6_001241"
     CDS             9665..11449
                     /locus_tag="ACG8M6_001241"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0456922.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11784..12636
                     /gene="licT"
                     /locus_tag="ACG8M6_001242"
                     /pseudo
     CDS             11784..12636
                     /gene="licT"
                     /locus_tag="ACG8M6_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            12733..14619
                     /locus_tag="ACG8M6_001243"
     CDS             12733..14619
                     /locus_tag="ACG8M6_001243"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0456923.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14660..15562)
                     /locus_tag="ACG8M6_001244"
     CDS             complement(14660..15562)
                     /locus_tag="ACG8M6_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0456924.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15792..17195
                     /locus_tag="ACG8M6_001245"
     CDS             15792..17195
                     /locus_tag="ACG8M6_001245"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0456925.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17195..18628
                     /locus_tag="ACG8M6_001246"
     CDS             17195..18628
                     /locus_tag="ACG8M6_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0456926.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18773..19699
                     /gene="trxB"
                     /locus_tag="ACG8M6_001247"
     CDS             18773..19699
                     /gene="trxB"
                     /locus_tag="ACG8M6_001247"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0456927.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19912..21639
                     /locus_tag="ACG8M6_001248"
     CDS             19912..21639
                     /locus_tag="ACG8M6_001248"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0456928.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21655..22005
                     /locus_tag="ACG8M6_001249"
     CDS             21655..22005
                     /locus_tag="ACG8M6_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0456929.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22099..22959)
                     /locus_tag="ACG8M6_001250"
     CDS             complement(22099..22959)
                     /locus_tag="ACG8M6_001250"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0456930.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23051..23479
                     /locus_tag="ACG8M6_001251"
     CDS             23051..23479
                     /locus_tag="ACG8M6_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0456931.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23641..23817
                     /gene="rpsU"
                     /locus_tag="ACG8M6_001252"
     CDS             23641..23817
                     /gene="rpsU"
                     /locus_tag="ACG8M6_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0456932.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23844..24290
                     /locus_tag="ACG8M6_001253"
     CDS             23844..24290
                     /locus_tag="ACG8M6_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0456933.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4353 c         5187 g         7090 t
ORIGIN      
        1 ggtttttcac atttttacat aaatttgtct taatatcgta gatgtaagct tttgaacacc
       61 atttattcgg aaaaagggag ctgtgacaag atttttgtcg cagctccctt taattctgat
      121 taatctatta gatcaatcat ctttatcgtc tgattctttg gtctctggat tcgctctagc
      181 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcagtaccgc ctacgccgaa
      241 tttaaaggcg cttttcttcg gaccattttt tgcccaaagt gactccactg tttttcctgg
      301 cgtttggatg gattttttat tgacagtaac ttttccagct gtttgtcctg tgaacccaga
      361 tacttttttc ttcatcacat cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc
      421 agggtttgct tggtaagctt gattgattag attggcaaga taattagccg aacgtattct
      481 tgcttggctg tccattccag tattgtcatc gtgtcctgcc caactactga tagtgataga
      541 tggagttgag acgatcagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
      601 ccaatctgca tttcctagat ttccgtttaa gcttgagatg acatctttga atggtgtagt
      661 gattttttca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttcgga
      721 ataaatacgg acagaattac tttcatgttt ataaatcaca ttacctgcat tatctgtgat
      781 ggaatcaatg atatagcctt cttcgtagac tccaccattt gctaaggcct ggaatccatt
      841 tgtctgttga agcgtcgtta catctactgt ccctaaagga gccgattcaa cgccccaatt
      901 atcatttgct gggtagttca tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc
      961 accggaattc aacacatctt gatataagtg ataagctggg atattgttgg accattccag
     1021 tgactcacgg acggtttgga aggtgttcga cccttcattg gtcgcattga cgattttctc
     1081 accagcattt tttccttctt gccaagtcgt tgcataatct gatacacggg attctgatcc
     1141 gacaagtccc atatcgattg caggaccgta aacaagaacc ggtttgatgg atgaaccagc
     1201 ttgacgttct gtatcaaagg cgtggttatt ttgattctgg ctgtaattcc gaccacccac
     1261 gaagccatag atacgtccgg ttctgttatc catcaataca tttccagtct ccacttggct
     1321 accagcacca tcatctaaca tgtagccata attttctacg ccggtttgta atgcagcata
     1381 gatatcttta tcgatcgtcg aatggatcgt atatcctttg ttttggatcg tttgctgtgc
     1441 tttttgataa taagcttcac tgaccgatga atctgacata tcagcattat ctttttctgc
     1501 caattgttgt gcaataatcc ttgttgcttc gttcatcacg gtataataca agaattctct
     1561 gtcagtctgt tccgcgattt gttgtgggag aaagtctttt gtaagatcat aggctttcgc
     1621 ttcttcgtat tgttctttcg taatttgtcc ttcacggtac atactgaata aaacaaaatc
     1681 ttttctggca agaccggcag aaagatcttc ttttagcgcg cctgtattcg tgtatggact
     1741 atacgtgata gggctttgag gcaaaccagc gatataagca gcttgtggca gagtcacatc
     1801 ctttgcatct acaccaaaga ttcctctagc agcttcctgg actccagcaa tattttgccc
     1861 cttattattc cgaccaaatg gtgagacatt caaatacgta gtaatgatct cattttttga
     1921 aaagtgtttt tctacttgtg cagaaagaac gatctcgttt gctttacgtt tgaatgttgt
     1981 ttcatcggtt aggatctgtt gtttgactaa ttgctgtgtc aaagtggatc ctccagacga
     2041 gccgatgcca gtcgcttctg aaactagtgc tcgtaaaaca gctttaggga cataaccttg
     2101 atgtttatcg aagtattcat cttctgtact gataatcgcg gttttcaata aaggcgaaat
     2161 cttatcagat gaaatagtcg tccgcatcag atctgtctgg atttttgata tctctgtatt
     2221 atctgcataa acaatctttg acgtttccgt aatgtttcct agttctgtct gcagttcttt
     2281 tttagtcggc aattgtgtgt cttcgacaag ataggcaaag tagccagcac ctattccgat
     2341 acctagagct gcgaacaaca aaagaagaac agtaataaaa acagtcaatg attgaaacac
     2401 tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct gtttttttcg
     2461 agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc taattatagc
     2521 aaataatagg acaaaaaaac agacaaggct gtttttgaca ggaatttctt tttcgaatga
     2581 gataaaacgt gaggaaatga tcttttttat gaaagagttc ttaagctttt ctttagggaa
     2641 agtttcagtt actatggaga acagggcaaa gaaaaacaaa ccattttctt tgtacatcat
     2701 cttatcaata tacaggaatt gttcgaaaca attgcctttc aaggaggaat ccatccatgt
     2761 tattaggctc acatgtcagc atgagcggaa agaaaatgct gttaggcgcg gcagaagaag
     2821 cagccagtta cggatcttcg acctttatga tctatacagg cgctcctcaa aatactagaa
     2881 gaaaagctat cgaagagatg aatattgatg ccggaaaagc atttatggca gaaaacggac
     2941 taacaaatat cgtcgttcat gcgccttata tcattaattt gggaaatacg atcaaaccag
     3001 aaatgtttcc atttgctgta caatttctga gagaagagat tgtccgtgcg gaagctttag
     3061 gtgctactca aatcaccctt cacccaggcg ctcatgtagg agcaggggca gaagctggga
     3121 ttgctagaat tattgagggg ttgaatgaag ttttgacaaa agaacagacg gcacagatcg
     3181 ctttggagac gatggcaggg aaaggtacgg aaatcggtcg aacatttgag gaattagcgc
     3241 aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgat acatgtcata
     3301 cgaatgatgc cggatataat ataaaagaag atttttcagg tgtaatggaa gagtttgata
     3361 aggtcatcgg attagatcgg cttaaagtac tgcatatcaa tgattctaaa aatccacaag
     3421 gttctcataa agatcggcat gccaatatcg gttttggaac aattggattt gacacattga
     3481 acaaaatcgt ccatgatcca atgtttgaca acatttcgaa aatattagaa accccatatg
     3541 tcggtaaaga taaaaagaaa gcttacgcac catatggaaa agaaatttcc atgttcaaag
     3601 cgcaagcatt cgatcccgaa gtgttccctg agttattagc agaagaagtc acactttaag
     3661 ttttctacct gacagcaaca aaaaaggatt gggcagtctg ccttgtcctt ttttgtttaa
     3721 gaaacaaagg agtgcttttt gcattcgttt tcgccatgct ccttttgccc cctcttttta
     3781 aattcagaca aacagggtat actgatagaa aagtaaaaaa cgaaaagttt cacaaacaag
     3841 tagcaaaacg tgattgaaag gacgaaaaga tgctgaacat tttaaacccc ttgatcgacc
     3901 aactaaaaaa gaaacataca tactttattc ttggcatagc cgtattattt acaattattc
     3961 ggttgtattt atatagtaaa gcaatctatg gaacagatat gtcggcgacc tatgatgatc
     4021 agctgctgat tcactatgct caaaatattt tggatggtaa atggctaggg gagtatacga
     4081 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
     4141 atctttctta tgcagttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
     4201 tcgcgtttaa accgattgtg aagaacaagt atcttctttt aagtatctat ttttattttt
     4261 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
     4321 tgattccggc tgttgagatc attatcgctt gtttattagc tatttattac cgcaaatacg
     4381 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
     4441 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
     4501 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
     4561 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
     4621 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
     4681 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
     4741 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
     4801 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
     4861 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
     4921 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
     4981 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
     5041 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
     5101 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
     5161 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
     5221 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
     5281 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
     5341 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
     5401 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
     5461 taatactgac acaattggtt tttcttttcg gtgtctcatg gttttgttct tatgcgccag
     5521 aaaaaaagga ctatttttta agtgtctaca ctggtgcagg cgtgccatta acgcaattca
     5581 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaagct ttatcttcta
     5641 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
     5701 aaatcagttt ttattcgatt tcttctaccg tttcctgcaa aagctgttca tattttttgt
     5761 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
     5821 cataagcgat gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg ccgaagaaac
     5881 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
     5941 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
     6001 tggtcatcat cagtaaagga atcatcgaaa atggcaacgc aaaagccaag aacacctggg
     6061 attcattcat taagttattc aaagcggcat gttcttcgat cgttcctttt gtacttgtaa
     6121 acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc
     6181 aaatcgggat acgcatatgg atgaatcctt ccatgatgac ttgaccggtc aacgtaccag
     6241 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gacaagatac
     6301 cagattttgc tacagcaatc aaaataccat tgctcatagc tgatggatca gataaagctt
     6361 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
     6421 tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta gaatcccaag
     6481 tagaaaaacg tacagcttgt gctacatctt cttcgttttg gtgatcgatc ttccgagttt
     6541 gtgaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
     6601 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
     6661 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
     6721 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc
     6781 cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca
     6841 ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcgatgt
     6901 ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat gtcttacttg
     6961 tccgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat tttgctgcca
     7021 tatactgtaa aagcatagca attagactag acatcaaaat aactgacatc aataaatatt
     7081 ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata taaccaactg
     7141 ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt tttccctttg
     7201 gtacatccac tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc
     7261 gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct ttcctattta
     7321 tctacgatta cattctacac ctcagaaaga aaaaattaaa gaaaaagatt cggtatgcct
     7381 aatttttttt cgtggtgtgt cttgctggtt aatgaaagaa attttgttct tctctaaaaa
     7441 aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt
     7501 aaaataaatc attgagagca attcggaaat agaaaggaga gatttatatg gtatcaacag
     7561 gtattgtcgt acttattgct attattgctt tgctaatcgg cgcagtaggt ggctttttcc
     7621 tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt
     7681 tgcgatcaat gatgatgtca atggggcaaa aaccatctga gaagaaaatt cgccagatga
     7741 tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaaagactt tttgcgggct
     7801 gattttttca gtccgttttt tatttcgtaa tttgttagac tattcagact attcagcaaa
     7861 aaagacaagc acagacaatc ctatgctctt gataaagaca aaggaggagt aaaatgtcga
     7921 tttttagaaa attaggttgg ttttttcgtc aggaaaagaa acattattta attgggattt
     7981 tctccctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt gggatcatca
     8041 ttgatgagat cgccgataag aatttatcaa taaagatcat ttcctggtgg atcttattat
     8101 tgatccttgc agctttctta caatatcttt ttcgttatat ctggcggacg aatatttggg
     8161 gaagtgcagc tcggttggag agagaacttc ggcagcagct attcaaacat tttacagaaa
     8221 tggacaatct cttttaccaa aaatatcgga ctggagattt gatggctcat gcaacgaatg
     8281 atttgaatgc aatccagaat gtagcaggag ctggaatctt gacctttgca gattcagtga
     8341 ttacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc ttaacactga
     8401 ttgcgctgat tcctctgcct ttgctagccg ttacttcacg agtgctgggg tcaaaactcc
     8461 acgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag acacaagaaa
     8521 gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat ctcgcggatt
     8581 ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt ttagactcct
     8641 tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca attattttag
     8701 gtggaaagta tgtcttggaa ggcacgatct caatcgggca gatggtctca tttattagct
     8761 atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac gtattggaaa
     8821 gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaagacg catatcgtgg
     8881 aacaactggg tgcagtgcag accccggcaa aaggcgagct gaatgtagct atttcctctt
     8941 ttaactatcc gaaagaagaa tcaccagcat tagaacaaat caaatttgcg atccaagaag
     9001 gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacaatc atcaaattac
     9061 tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt tcgatcaaag
     9121 aatattcatt gaatgcttta ttaggatcaa tcggttatgt gcctcaggat cacttcctgt
     9181 tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca gaagaagaag
     9241 tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaattg ccaaaaggtt
     9301 atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa cagaggattt
     9361 cgattgctcg agcattgatc aaaaatcctg aattgctgat cttagatgat gcactttctg
     9421 cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata agaaaagaga
     9481 aaacaaccat cattgcagct caccgtttaa gcagcgtgat gcatgcaaaa gaaatcattg
     9541 ttgtcgatca agggaaaatc attgaacgag gaacccacca cgagctgctt gcattaggtg
     9601 gctggtatga acgaatgtgg gaaaaacagc aattagaagc gaagatagaa ggaggcgagg
     9661 cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta aagaacagaa
     9721 gaagatcatc aaacgactga tgaagttcgc caaacctttt agaagaacgt tcatcgttgc
     9781 tatcttattt gcgtttgccc tgtctgtgat caatgtccta ttgccaagga tcatccaaac
     9841 ttttatggat gatcatctgg ctaaacagtc agctacgaca caagttattt tgttttttgc
     9901 tggtgtgtac ttgtttggag tcatagtcaa aagtatcatt tggtttttcc aatggtattt
     9961 gtattccatg gcttcattga agacctatca gtatgttcgg gtaagattat ttgaaaagct
    10021 gcatacacta ggtatgcgtt attttgacca gacaccggca ggatcgaccg tttctcgagt
    10081 gacgaatgac acagaaacgc tatttgaatt ttggtatgtc tttttgatgg tattgactgg
    10141 gatctttgca gtcgtttctt ctttcgttgc catgtttcaa atcaatggag aaattgcctt
    10201 gtggactttg gtgttcttgc cgattttagg aatcgtcatc tggtattacc agaaattcag
    10261 ttctcgtatt taccgaaata tgcgggaaaa attaagtcag ctgaacacga aattgaatga
    10321 atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt tagctaaaga
    10381 atttgaacaa gtgaacaacg agtatttaga cacgcgctat gcgatgatca aaacgaattc
    10441 gattctgtta agtccaatca tcaattttct ctatgcattg gcgattgcgt tggcgttgac
    10501 tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct atgcatttac
    10561 gacctatgtc caggaatttt ttaatccaat gtctcaaatg atggactttt tgagcacctt
    10621 tacagatggg atggttgccg gtagtcgtat tttgaaaatc atggatacag aagaatatgc
    10681 accgcaacaa aatcctggag cagatcaagc gattacttca ggtaagatcg agtttcgcaa
    10741 tgtaagcttt tcttatgatg gagagcacaa tgtattgaac aatatctctt ttgtcgctaa
    10801 tccgggtgag accgttgcat tagtggggca caccggaagc ggaaaaagct cgatcatcaa
    10861 cgtattgatg cggttttacg aattttatga aggccaaatc ttgattgatg atcatgacat
    10921 tcgagaatat ccaatggaag agttacgtaa gaaaatggga ttggtcttgc aagatgcctt
    10981 cttgttttat ggagatattg caggaaatat ccggatgatg aatccagcta tcacagacga
    11041 acagattcgc catgcagctg agtttgttca agcaaacaag ttcatcgatg aattaccaaa
    11101 aggatatcac tcaaaagtga tcgaacacgg tgccagctat tctagtggac aacgacagct
    11161 gatcactttt gcgcgaacaa tcgtgacaga tcctaaaata ctagtattgg atgaagcaac
    11221 agcaaatatc gatactgaaa ccgaaggatt gatccaagaa ggattggcaa aaatgcgaca
    11281 aggacggaca acgatagcga ttgcccatcg gctgtccacg attcgggatg cagaattgat
    11341 tttagtttta gacaaaggtc atatagtaga gcgtgggaat catgaaacac ttctagcgca
    11401 aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtgtgtaaa aagaggttgt
    11461 gacagtagca tagtagcatt ttgccctaag cgctagagtg aaaataaaac agagcttcgc
    11521 agattcttca tctgtgagcg ttagtggcgt agtcacccga tttaaaaaag acgacgacag
    11581 ctagtcaaaa gcggctaata accactaaga aagcgttgac aaaagcgttt gctttcgata
    11641 atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag
    11701 tctcccatgg ttccatggac ggctttttgg gtttttcttt ttgttttgga tacagcccta
    11761 tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa
    11821 taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa
    11881 agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag ttgacaaatg
    11941 acgggctttc acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa
    12001 tttcttgtga aatcattgaa tatacaaaag aggctctaca agtccagttg aatgattctg
    12061 tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa atgggaatat
    12121 ttgtaccaaa tgtattgctg tatgatatca aacaactatt tcctaaagag tatgccatcg
    12181 ggaaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagatgaa
    12241 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca acaagacaat
    12301 acctacctgt tcactataac aatacaggat attctaaata tcgtccgcta ttactataag
    12361 attacatttg atgaagattc agtccatttt tttcgattcg tcacacactt acgttttttt
    12421 ctgacacgcg tcctagatgg agaaaaatca gaagtgggag aagtttctga ggaattgttt
    12481 caggttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
    12541 ttagccgaga cgttcaacta ccagatgtcc acggacgaaa aaatgtattt aacgatccat
    12601 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaaggagg ataaattaac
    12661 aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg
    12721 gagggaatag aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc
    12781 gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa
    12841 aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg
    12901 cgagcgggag ggcagtatca agttgtcatc gggaatcatg tgccagtcgt gtttgaagag
    12961 gttataaaag caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg
    13021 tttgatatgc ttattgatgg aatatcgggc tgtttccaac cattccttgc tatcctagct
    13081 gctgacggga tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc
    13141 ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct
    13201 gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg
    13261 cttggcgcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa
    13321 gcaccactaa cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta
    13381 ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt
    13441 gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg
    13501 aatttcttcg ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt
    13561 ggaccagtat tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca
    13621 ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt
    13681 ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa
    13741 aatattatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc
    13801 tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt
    13861 attgtgggga ttacagagcc agctatttat gggtttacat tgccacgtaa aaaaatattt
    13921 atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact
    13981 tcttggaatc aaggtggact aggcatcttt acaattccca actatattag accagatggt
    14041 gatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt
    14101 gcgctgactt ttttcttttg gaaggaggaa gcagatgaaa cagatgatag acagaaaaga
    14161 tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact gaatgcagca
    14221 aaggatgcag cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa
    14281 ggagaagtac gtgccccttt tgacggaaca gtcatgtccc tgtttccgac gaaacatgca
    14341 atcgggatgg tttctgaagc aggtctggaa ttgctgattc atgtcggatt ggatactgtt
    14401 cagttggaag ggaaatattt tgaaagcttg gtgcagcaag gagaaagagt gaaacaaggc
    14461 gatctgttgc ttcgcttcga caaaaagaaa atcgaagagg aaggatacag tttagaaaca
    14521 cctgtgattg tcatcaatta catggattat ttggatattt tggaaaatca aggggaaacg
    14581 gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg aaagaatgga
    14641 ctaggcttaa ctattctttt tagtccactt ctttattttc aaaattgaaa ggctctttca
    14701 tgatggctga gctgataggg cgtttatcaa atttttgtcc tacagaaatt agatggacta
    14761 aattctgttg ataattttct gcaaaaacac aactatataa cacgtctaga agataacaaa
    14821 tcgatgtatt gatcgtaaaa ttaccaattt tcgaatacag tcgttcgcgt gtcgtaagtc
    14881 gcaaagcggc tgtactcatt gaagatagcg ttgaatcacc gatactggtc aaagctaaaa
    14941 ttggcacatg attttgtttc aatatttctg cggtacgaag gatcacattg ttttcacccg
    15001 tataagaaat cagaatccca cacatatcac ttgtgcaatt gtacgcttca tagaacgatt
    15061 caccaaaagt ggtgacaatc gtgacatcca ttttgatgcg cttcattttc agcatgaaat
    15121 cttgagaaat caaagtattc gagttactgg cgaaaatcac gatttttttt gcttgaagca
    15181 ttagttgttt ggctttttgc agttggtcat gatgaatgag agaaagtgta tcttgaatcg
    15241 tcatctgttc taaagaagcc attttatttg cgatcgtcat aataccgtct gtggtggaaa
    15301 aaggaagatt cgcatctacg tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa
    15361 aagcattacg aaaactgacc cagccatcaa aacccatttt ttttgctaca cggatgagtg
    15421 tggacgggtg gacataacat cgctcagcaa tctcttggat ggtcatttcg ccaagtgctg
    15481 gagaatcgct taagagaaac tgaatcactt cattttctga aggagaaaag gagatttctt
    15541 tgattttttc acgtaataac atgtagatac cttcttcttg tttttttacg taaatcataa
    15601 cttttttgaa aatgatttgt aaacggattt atgttatata gcttaataaa gacatttttt
    15661 gacaagatat ttacaaaaat ccagaagaaa acactaactt ttgtaaatgt tactggaaag
    15721 aaacgaattt ctattgtttg ttttgtttac aaaaaagata ataaaagtgt aaaaagtaaa
    15781 ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca
    15841 cccccgagtt gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct
    15901 ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat cgtcagtcag atgtctcagc
    15961 gtatgtggga tgcttctccg tatgaagtca aaatctatgc aactctggat cgggaagaag
    16021 cattgaaaga tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa
    16081 tcaaagacga gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg
    16141 gcgggatatt caaagctttt cgtaccgttc cagtggtttt ggcaatcgtt gaagacatga
    16201 agcgtttatg tccaaatgct tggctgatta atttcgcgaa cccaagcggg atggtgacag
    16261 aagcagttat cagatatggc aaatgggata aagtgatcgg attatgcaat gtgccagtaa
    16321 tggcgatgat gacagaacca acaatgcttg gcaagaaaaa agaagagctg atctacaagt
    16381 ttgccggtct aaatcacttc cattggcaca aagtagcaga taataaagga aatgaccgca
    16441 cggcagaatt gattgatcgt ttatacgatg aagacaatgg attgccgaaa aatatctttg
    16501 acatcccgtt tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc
    16561 gctattacta tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg
    16621 gtacaagagc gcagcaagtg aaagaaacag aagcagagct gtttgaatta tacaaagatc
    16681 cagaattgga tcataaacct gaacaattgc aaaaacgagg tggtgcttat tattcagatg
    16741 ctgcctgtga aacgattgct tcgatctatg gaaataaaga aacacagatc gtggtatcta
    16801 caaagaatga tggagctgtt cctgatctgc cggcagattg tgtagtagaa gtcactgcct
    16861 atgttggcgc acaaggcgca cgaaatgtag cgtttggttc ccttcctgct gctgaaagag
    16921 gctggttgca agtaatgaag aatatggaat tgctgacgat cgaagcagcc gtgactggtg
    16981 attatggaac agctttgcaa gcgtttacga tcaatccgct tatacgatca ggaaagacag
    17041 caaaacggat catggatgag ttgtttattg cacatcagca gtatttaccg caattcaagg
    17101 aaacaatcag ccggttaaaa gcagaaggaa tcgaagtcca agatgaactg gcaagagaat
    17161 tatcaagcaa tacacatgta atggaggaag tatgatggga ttcagaaaag attttttatg
    17221 gggcggagct acagcagcca atcaatgcga gggcggttat gatgaaggcg gacgtgggtt
    17281 agcgaacgta gatgttgtgc caatcggaga agaccgtttt cctgtcatta ctggaaaaaa
    17341 gaaaatgttt gaatttgatg aaaatcattt ttatccagca aaaattgcga tcgatatgta
    17401 tcatcattat aaggaagata ttgctttatt tgcagaaatg gggttcaaga cttatcggat
    17461 gtccattgct tggagtcgta tttttccaat gggcgatgaa aaagagccaa atgaatctgg
    17521 gttgaaattt tatgaggatg tctttaaaga atgtcaaaag tacgggatcg agccgcttgt
    17581 gacgattaca cattttgatt gtccgatgca tttgatcaaa gaatatggtg cgtggcgttc
    17641 cagagaactc gttggttttt atgaaaatct gtgccgagtc atcttcaatc gctataaagg
    17701 acttgtcaaa tattggttga cattcaatga gatcaatatg atccttcatg caccattcat
    17761 gggcgcaggg ctgtattttg aagaaggaga aaatgaagaa caaatcaaat atcaagcggc
    17821 acatcatgag ttggttgcaa gtgccatcgc aacgaagatc gctcatgaag tagatccgga
    17881 gaacaaagta ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga
    17941 agatgtttgg gcttctagaa agtcagatcg agaaaattat ttcttcattg atgtccagtc
    18001 acgaggagaa tatcccgctt atgcattgaa agaaatggaa cgaaatggca ttcagatcaa
    18061 aatggaacct ggggatgccg cattgctgaa agaacatact gttgatttta tctccttttc
    18121 ttattattct tctcgagttt cgactactga caacgaactc ttggaacaga ctgcaggaaa
    18181 tatctttgct tctgtcaaaa atccatatct ggaagcaagt gaatggggtt ggcaaattga
    18241 tccattaggc ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccactatt
    18301 tattgtagaa aacggcttag gtgcagtcga tacgcccgat gagaatggtt atgtggaaga
    18361 tgattatcga atcgactatc tggcagcaca tatccaggca atgaaagatg cagtagaatt
    18421 agatggagtc gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg
    18481 tacaggtgag atgaagaaaa ggtacggatt catttatgtc gatcgtgaca acgaaggaaa
    18541 tggcacatta gcccgttcga agaagaaatc attcaactgg tataaaaaag taattgattc
    18601 aaatggagaa aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag
    18661 cggatttttc gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt
    18721 tcgcattggc tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga
    18781 tgtaatcgtt attggcgcag ggcctgctgg gatgactgct gctttatatg cttcacgttc
    18841 aaacttatcc gtattgatga tcgaacaagg cgctcctggc ggccaaatga acaatacggc
    18901 agaaatcgaa aactatcctg gtttcgattc aatcatgggt cctgatctag cttataagat
    18961 gtatgaaggt gtttcaagat ttgggacaga aaatgcttat ggaatcgtgc aagatatcaa
    19021 ggatcacggc agccacaaag aagttatttg tgaagataaa acttacgaag gaaaaacagt
    19081 cattatcgca acaggctgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc
    19141 tggacgtggc gtttcttact gtgcagtttg tgatggtgca tttttccgta acaaacgtct
    19201 agtagtgatc ggaggaggag actcagcagt agaagaagcc atctatttga cacaatttgc
    19261 ttctgaagta gtcatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga
    19321 ccgtgcattt gcgaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt
    19381 tggaaatgaa atggtagtga caggtgtgaa agcacgtaat gtcaaaactg gcaatgagtt
    19441 tgatttggca gcagacggag tattcatcta tgtaggattg gatccattaa ctgcaccatt
    19501 tagagaagct gggttgacga acgaagccgg ctggattcca acagacaacg aaatgaagac
    19561 gaatatacca ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac
    19621 tactgctgtt ggtgatggtg ggattgctgg acaacaagtg tataaatact tggaatcttt
    19681 agaagaaaca actgcataag tttaaaaaga gtgaaagaac ggtttcaatt gttcttttac
    19741 tctttttttt gcgtgaaatc tactgaatcc gatttatgcg aatacttgta accgtgcttg
    19801 ataaaagaat cttttcttga tttaagcgtt ttttggtcct catactttca tatctaataa
    19861 gtgaatgcta tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg
    19921 gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa
    19981 ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa
    20041 ttcggtactg caggcatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat
    20101 acggtaagac aagcaacaga aggacttgca cgtttcatgg atacacaagg tgaagaaaca
    20161 aaacgccgag gtgtcgctat cgcttatgac tcacgccatc aatcaccaga atttgccatg
    20221 gaagcagcca aaacattagc acatcacaat atcccttctt atgtattcga aagtttacgt
    20281 ccaacaccag agctttcttt tgcagtccgt catttgaaga cgtttgcggg aatcatggta
    20341 actgcatccc acaatccagc agcttacaac ggatataaag tctacggaga agatggggga
    20401 caaatgcctc ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg
    20461 ttaaaagttg aagtattatc tgacgacgaa gctgaacata gcggattgat cacgatcatc
    20521 ggagaagaag tggatgccgc ttatctagaa gaaatcaaag cagtaacgat tgatcatgaa
    20581 ttggtggaaa caatgggtaa agatttgaag cttgtgtaca caccacttca cggaacagga
    20641 aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttcca
    20701 gaacaagctg tcgcagaccc ggatttcact acagtcaaat caccgaatcc agaagaacac
    20761 tctgctttcg agtatgcgat gcgacttggc gagaaagaag gcgctgactt actgattgca
    20821 acggatcctg atgcagaccg tttaggcgca gccgttcgtt tgccaaatgg ctcctatgaa
    20881 gtattgacag gaaaccaaat cggagccctg tttgctcaat atattttgga agcacaccaa
    20941 tcgcgaggca ctttacctga aaatgcagcg gtattgaaat caatcgtttc tagcgaattg
    21001 cctacagcaa ttgcggaaca ctatggggct accatgttca atgtattgac aggattcaaa
    21061 ttcattgctg aaaaaattca acaatttgaa gaagatcatt cccatacttt tatgttcgga
    21121 ttcgaagaaa gttacggtta tttagttaaa cctttcgttc gcgacaaaga cgcgattcaa
    21181 gcattgctct tattagcaga agtagcagct tactataaaa aacaaggcaa aacattgtat
    21241 gacggcttgc aaacgatttt tgaaacatat ggctactacg cagaaaaaac gatttctgtg
    21301 acaatgagcg gacaagaagg ctctgcaaaa atctctgctt tgatggaaaa attccgtgaa
    21361 gaagcaccga cagaatttgc cggagcaaaa gtcatccaaa cagaagactt caaagagttg
    21421 acgagaacag accgtgaagg aaacgtagaa aaactaacga caccgccatc tgacgtattg
    21481 aagtatacgc tggaagaaaa tggctggatc gctatccgtc cttcaggaac tgagccgaaa
    21541 atcaaatttt atatcggtgt aaaaggaaac tcttcagaag acgcagctga aaaagtagcc
    21601 gcttatgaag cgattatcaa aaaaatgaca gaagaataaa aaaacttgag actgatggaa
    21661 aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac
    21721 acgcaaaaaa caaaagtaga gaaagtcagc caactattca aaatgttgag tgaccccact
    21781 cgtctcaaaa ttctgctgta tctgaaagac ggggaacaaa atgtgacagc aatcacccaa
    21841 gcagtagaaa tggaacaatc tgctgtatcg catcaactgc gtttattaag agaaaaccat
    21901 gtcgtcaagt cccatcgtga gggaaaagcg attttgtata gtcttgatga ttctcacgta
    21961 ctggatattt tgaaccagac gctcaaacat gtggaacatc aataacgata ggtgcaaata
    22021 gagacaaact ggactgtatc aacgaatctt cttcgctgac acagtccagt ttttttaaga
    22081 aaaagacaag aaaaactgtt agtctgtttc ttcggtggtt tcagttccat aataactatg
    22141 atcttctagg tctaaagcat tcaaaataag tgaagctgtt tcttcgatag ataaagaagc
    22201 gacattgatt actacgcagc ccaatttttc ataaagatca ttcgcaaaat cgagttcagc
    22261 tcgtattttt tcgatatcgg aataagcagt atcaggattt aatccgtaag agcgcatccg
    22321 ttctttacgg atattgttta aaatatctgg gttattcgtc agtcccacga tttttttagg
    22381 atcgatctcc caaagctgtt tgggaatatg tgcttgtggg atcaaaggta gattggcaac
    22441 ctttaaattt ttgttagcta aaaacaagct caaaggcgtt ttggaagttc tggatacgcc
    22501 tagcaaaacg acatctgctt ctagaaaccc acgaggatct ttcccgtcat cgtattttac
    22561 agcaaattcc attgctttga tccgtttgaa atagttctca ttcaataaat gaagggcgcc
    22621 aggttgccga atgggcttga taccagttaa gcgctcgatt tctgatatcg gcggagtcat
    22681 cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa cgcgtcatct cgatcaattc
    22741 ttgattgatc aatgtgtgta ggacaagggc tttttctttt agtgcatctt gcagtgcctg
    22801 catcaatgtt ttctcatctg tcacaaatgt tctgcggaat aaattgaact caacggtagg
    22861 atattgggcc atagtggctt gcgctaattt tgtcgcagtt tctccagcag agtcagagat
    22921 gacaaaaaaa gtaatgattt ctgtagaatc ggaagacatg gataaaacct cacttgcatt
    22981 ttttttatgt ttattatacc atactctaga atgtgtactt cgtaatcatt ttgatttgga
    23041 ggaataagtc atgaaaaaac agacattgat tgaacaatca ctagaaacat tgaaaaataa
    23101 cggttttaaa tatactaaaa aaagagaagc attgttaact tatttagtga agcgcaatcg
    23161 ctacgtatcc gctaaagaag tttttgacta tatgaatcag gaattcaaag gagtaagtta
    23221 cgatacgatt tatcgaaatc tccatgattt tgcagaaatc gatttactgg aagaaaccga
    23281 tttaaatgga gagatgaagt ttcgttttca ttgctgtcaa ggggaaatcg gccatcacca
    23341 tcactttatc tgtacggtgt gcggaacaac gaaagaaatc catatgtgtc caatgaatta
    23401 tttcgaagaa cagctcggtg gatgtacgat cgaagggcat agattcgaga ttttcggaag
    23461 atgtgaaaat tgtcaataag gatttaaata aaataacatt tgataatttt aaaaaaaagc
    23521 aagatttcct tgacggtcaa aacagcatta gatataatat ttacaggaca gtcttgtttt
    23581 ttgagatata tttcaataac aattacggtt actttttaag ctcggaggga gggaattaac
    23641 atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg atgctcttcg tcgcttcaaa
    23701 cgttccgtgt caaaagcggg tactttacaa gaatctcgta aacgtgaatt ctacgaaaaa
    23761 ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa aacgcaaaaa attctagttc
    23821 tgtgatgaat tggagttggt agtgtgtcac tactaagtag attaaacgat gacatgaaaa
    23881 cagcgatgaa agcgaaagat aaggaatctt tacaagtcat ccgaatgatc aagtcatcta
    23941 tacaaaacga gcagattaaa gaaggtcacg acttgacaga agaagaagaa ttaacggtgt
    24001 tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga gtttgaagaa gcaggacgtg
    24061 acgatttagc tgaaaaagta aaaagtgaaa ttgtgatagt cgaaaaatac atgccggaac
    24121 aactttccga cgaagaaatt cgacaacttg ttcaagaagc aatcacacaa actggtgctt
    24181 catcaatgaa agaatttggc aaagtcatgg gtgcgatcat gcctaaagtc aaaggaaaag
    24241 cagatggaaa ccaagtaaat gccattgtga aagaactatt acaatcttaa ttttatcatt
    24301 taaaggctct tcgtcaatga gtgttggtga agaaattc
//
LOCUS       DAZKOB010000032        24228 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000032 DAZKOB010000000
VERSION     DAZKOB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.032"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..743)
                     /locus_tag="ACG8M6_001254"
     CDS             complement(9..743)
                     /locus_tag="ACG8M6_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0456934.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1070..1480
                     /locus_tag="ACG8M6_001255"
     CDS             1070..1480
                     /locus_tag="ACG8M6_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0456935.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1473..2174
                     /locus_tag="ACG8M6_001256"
     CDS             1473..2174
                     /locus_tag="ACG8M6_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0456936.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2174..3760
                     /locus_tag="ACG8M6_001257"
     CDS             2174..3760
                     /locus_tag="ACG8M6_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0456937.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3779..4000
                     /locus_tag="ACG8M6_001258"
     CDS             3779..4000
                     /locus_tag="ACG8M6_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0456938.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            3997..4758
                     /locus_tag="ACG8M6_001259"
     CDS             3997..4758
                     /locus_tag="ACG8M6_001259"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0456939.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5138..5647
                     /locus_tag="ACG8M6_001260"
     CDS             5138..5647
                     /locus_tag="ACG8M6_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0456940.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5722..6261)
                     /locus_tag="ACG8M6_001261"
     CDS             complement(5722..6261)
                     /locus_tag="ACG8M6_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0456941.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6401..7012)
                     /gene="rpsD"
                     /locus_tag="ACG8M6_001262"
     CDS             complement(6401..7012)
                     /gene="rpsD"
                     /locus_tag="ACG8M6_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0456942.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7274..8125
                     /locus_tag="ACG8M6_001263"
     CDS             7274..8125
                     /locus_tag="ACG8M6_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0456943.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8153..8788
                     /locus_tag="ACG8M6_001264"
     CDS             8153..8788
                     /locus_tag="ACG8M6_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0456944.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8808..9581
                     /locus_tag="ACG8M6_001265"
     CDS             8808..9581
                     /locus_tag="ACG8M6_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0456945.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            9989..10786
                     /locus_tag="ACG8M6_001266"
     CDS             9989..10786
                     /locus_tag="ACG8M6_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0456946.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10764..11177
                     /locus_tag="ACG8M6_001267"
     CDS             10764..11177
                     /locus_tag="ACG8M6_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0456947.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11161..13806
                     /gene="mutS"
                     /locus_tag="ACG8M6_001268"
     CDS             11161..13806
                     /gene="mutS"
                     /locus_tag="ACG8M6_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0456948.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13824..15932
                     /gene="mutL"
                     /locus_tag="ACG8M6_001269"
     CDS             13824..15932
                     /gene="mutL"
                     /locus_tag="ACG8M6_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0456949.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15954..16514
                     /locus_tag="ACG8M6_001270"
     CDS             15954..16514
                     /locus_tag="ACG8M6_001270"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0456950.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16599..18587)
                     /gene="treP"
                     /locus_tag="ACG8M6_001271"
     CDS             complement(16599..18587)
                     /gene="treP"
                     /locus_tag="ACG8M6_001271"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0456951.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18810..19523
                     /gene="treR"
                     /locus_tag="ACG8M6_001272"
     CDS             18810..19523
                     /gene="treR"
                     /locus_tag="ACG8M6_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0456952.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19600..20046
                     /gene="msrB"
                     /locus_tag="ACG8M6_001273"
     CDS             19600..20046
                     /gene="msrB"
                     /locus_tag="ACG8M6_001273"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0456953.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20216..20818
                     /gene="ruvA"
                     /locus_tag="ACG8M6_001274"
     CDS             20216..20818
                     /gene="ruvA"
                     /locus_tag="ACG8M6_001274"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0456954.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20831..21832
                     /gene="ruvB"
                     /locus_tag="ACG8M6_001275"
     CDS             20831..21832
                     /gene="ruvB"
                     /locus_tag="ACG8M6_001275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0456955.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21861..22439
                     /locus_tag="ACG8M6_001276"
     CDS             21861..22439
                     /locus_tag="ACG8M6_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0456956.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22491..22973
                     /locus_tag="ACG8M6_001277"
     CDS             22491..22973
                     /locus_tag="ACG8M6_001277"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0456957.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23090..23464
                     /locus_tag="ACG8M6_001278"
     CDS             23090..23464
                     /locus_tag="ACG8M6_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456958.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23839..>24228)
                     /locus_tag="ACG8M6_001279"
     CDS             complement(23839..>24228)
                     /locus_tag="ACG8M6_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0456959.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT         7890 a         4346 c         5192 g         6800 t
ORIGIN      
        1 gcttttgatt aagccataaa taataattta tataagaata ctgctacgac accagcacag
       61 ataggtgcta caactggtac ccatgcatat ccccattgag aagaaccttt atgtttcaat
      121 ggaagaagtg cgtgtacaag acgtggagca aagtcgcggg ctgggtttag tccaggacct
      181 gtaggaccac caagtgaagc aactaatgtc catacaagga aaccaagtgc taagtgagct
      241 gtaccaagat tatcttggaa gaatggtgct tttgtaatac ctagggcacc aaagaataaa
      301 acgaatgaac caaagaattc attgacgaaa ccattgaatt tagaaccaac gctgttgatt
      361 gttgagaatg tacctaacac atggtttacg tcttctgttt ggtcgtagta aggtttgtaa
      421 caagctacta cgatcaattg tccaatcgct gcaccaatca attgtgcaat gatataagga
      481 acaacttcag cccaagggaa tagaccatta gatgctaaag caattgtaaa tgctgggttg
      541 atgtggttac cgctgattcc accaaaaatc attgctggca tcataacccc gaatccatac
      601 ccgacagcaa tcagcatcca gtcactgcca tatccttttg taccttttaa atctacgttt
      661 gctacagcac cgttacctag aacgattagt aatgctgtac caatcatttc cgccgcaaga
      721 cgggtcatta gtgagtaatc cataaatacc tccaaaagtt ataaaaatgc aaaaaaaaaa
      781 gaaacatcaa cacgaaaagc atcggtgcag tgtttcgtgt cggaaggatt tctgcagtct
      841 taacaaatgt gaataaaaaa atgaattcca ttacttataa gaaaccagac caacagtaaa
      901 actccaactc tttttaaaag ttctccacaa atcaccaagc ttaactataa atataccaaa
      961 aaaaaaacgt tttttttctc aaaaattgta tcttagaaac agcatgtttt gaaactttca
     1021 gaaaaggttt gttagtaaaa tgaaagctat acgtaaggag tgttgtttga tgaaattttt
     1081 tcaagagttt ttcattatta ttttcttttc tgtattagga gaaggcatac ggatttttgc
     1141 tcatttacct attcctggaa gtatcttagg tattcttttt ttatttttag catttgaaat
     1201 gcatcttgtt gatcctgaaa aaatcggagc aactggtgat tttttattga ataatctagc
     1261 cattttattt gttcctgctg gtgtaggttt attggaatat tttgatgata ttgcttcgat
     1321 atggcctgtt ttactgggag cagtcgtcgt gtgttccatc gtcacgatgg ttgcagcggg
     1381 aaaaacggca gaaggtgtag aagctttgct gggctatgtc cgtaagaaaa aagcagaaag
     1441 ggtatctgta aacgaaatgg aggagaatca aattgactga gttttttgtg aatccattat
     1501 ttggcttaag tttgacttta ggagtgtacg tattatctga caaatttttg cggaaatata
     1561 aactgcctgt tttgaatcct ctcgtattat cgattgttgt tattatctta tttttgcatt
     1621 tttctgggat cagctataaa gactataata tcgggggcaa ttttttgact atgctgatta
     1681 ctcctgcgac agtggcactt gccatccctc tttataaaaa cttccgttta ctgaaagaga
     1741 atttttatcc tgtaatggct gcgattttag caggaatagt cgcaaattgc ttggttagtg
     1801 tagggttcgg ttacttcttc tctcttcata aagagatggt gatctcatta ctacctaaat
     1861 ctgtaacgac ggctatttct gtcgatttat ctcatacgat gggcggggtg aatgccgtga
     1921 cacttgctat tgttgtctct acaggtattt ttggttcgct gatcgccact catattttcc
     1981 gcctattcaa gatcgaaagt ccagttgctc gcggtgtggc gttaggaagt acttctcatg
     2041 ctattggtac agcaaaagca atcgagattg gtgaaataga aggaatcatt tccggacttg
     2101 cgatatgcgt caacggaata ttgaccgttt tattgctacc gttattcttt cagccttttg
     2161 ctggcttgtt ttgatgcgaa aaccactatt ttatgctaca ataaccaaaa cgttcgagca
     2221 taaaaaagga gggaaagacg tgtatttgtc ttccgagtat cttaaacaat ttgataaaga
     2281 tctatattat aagattctcc tcctagaaag ttttgaggat caagcttggc ataccgctgc
     2341 acaattagcg caagttgttc agctggatgc ccgcagcgtg tcgaaatacc tgaacgagct
     2401 gtctaaaaac tatcagcagt ttagcggtaa gacacaccct ttgtttacaa aaaatcaccg
     2461 ttctggttat aacttttatg atacgctcga ttcaattgaa catgagcgtt ttttgatcta
     2521 tctcgtgcaa agtacgctga aattccagtt gctgcacgac attttttttg aagaattcca
     2581 tacaatgtac caatttgcgc aaaagcatta cattagtgaa tctactgctc atcgaaaaat
     2641 caatgaatgg aaacagcaac tgcaaactta cggaattcgg ctgcaacgag gtacatatat
     2701 cgctcaaggc gaagaagaaa tcattcgact ttacttgcat atgacattct ggcagttgtt
     2761 tcgagggaaa atctggccat ttgaaacgat ttctcaaatg gacgtaaaga atatggcaga
     2821 acatatcatg gcttttttta atgttcgtct gaacgagatc aaaaaaagac gattggaata
     2881 tatgctaggt gccttttttt taagaaaatc acagaagcat tatgttgtat tgaatgagaa
     2941 aaaaagacgg ctgatttctg acaatctgtt gttccagcga ttttgccagg tcatggagcc
     3001 ggtatttcct aattattttc aagtagaaga cgagttaggc gcattatttc ttgtactgat
     3061 gactcgtgaa gaatattata gtgatcctaa gatacggaag aaaatttttg attttcatca
     3121 acaagcaaaa acaccgccct ttacagccct ttcagaagca aaagccgctc tttctcttta
     3181 tcaggaagag caggggctgc cagcagaaaa tctaaccttc gaggcagaaa actatttatt
     3241 cagcagtcat tttttcgcct atctttttcc taatgcgaaa gaaacgattg atggcaatag
     3301 tagtgatttt attaatcatt tagttattga aaacaaggaa ttgaaacaat ggctcgtaca
     3361 tttttttgaa agtagacaca aacatcccaa tcatctggca tttaaaaatc atgcgttttt
     3421 gatggggcgg tatctgactg tgtttaaaac attaggagcc tttactccac agcttccaaa
     3481 gatcacgatc cttttaatga cagatttccc attatttgaa gagcaactgt tagaggaggg
     3541 attacggaca tttttccgta atgagtatca gctgattttt ctgccaacag attatcgggg
     3601 gagagaagtt gatcttctga tttcgacaag taaagtccac cgcaaaccat gggcggatct
     3661 tgactatttt attgtgactg aagaactaaa attaattgat tacatccaac tatctcaaaa
     3721 atttgaaatg attcaaaaac aaaaactatt aaaacaataa caaagaagga gaaaaaagat
     3781 gacatttgaa gaaattttac cagaattgaa aaaaggtgca aagattattc gtaaaggatg
     3841 gagtggattt gaactttatg ttgttctcgt atccgatgat gaatttgatg gctgtccggt
     3901 gacgccgtac ttattgatca aaacaagtga cgaaggtttt tccagcttcg ctcctacagt
     3961 ttgtgatatt ttagcagatg attgggcgat cgtagaatga aaaaccagta tccagatcta
     4021 gctaaacagg ttgtatttat cacaggagct gcttctggaa ttggtgctgc gcaagcccga
     4081 gcgtttcttg aacaagatgc ttttgtattt ggattagatc aaaagttcgg taaaatggaa
     4141 gaaatttctt tgcgatttcc tgaaactttt gcctacgcac taggtgatgt tcgacaaatg
     4201 aaagaactcc aacaagcggt atcgaaatgc caaaatcatt ttggtgaagt tacgatttta
     4261 ctgaatacag cggggatatt ggatgcctac aaacccttga tggaaactga cgaatcgctg
     4321 tgggatctga tttatgagac gaatgtgaaa agcatgtatc agctgacaaa aatcatcctt
     4381 cctgatatga tccgccaaaa aagcggaacg atcatcaata tggcttcgat tgctggcttg
     4441 attgctggtg gaggtgggat cgcttataca agcgcgaaac atgcgattgt cggatttacc
     4501 aaacaactcg ctctggatta cgcaggacag ggtatccgag taaaaggaat tgctccagga
     4561 gccatccaaa caccaatgaa tgcggctgat tttgaaggga aaggtgagat ggcagagtgg
     4621 gtggcaaatg aaacaccagt caagcgatgg gcacagccgg aagaagtagc tgagctgacg
     4681 ttatttcttg cctctcctca agcaagctac atacaaggaa cgatcatccc aatcgacggc
     4741 ggatggttgt taaaataacc ttgtgttttt ttcaagcttc tgtatgataa aactaaagag
     4801 agaaacaacg gttgatgata aaaaaagtcg atgactgttc ccgacattcg atgccgaaca
     4861 gccagttcaa aaaattcttt ttaataatcg ttaatattcg aaactaagcg gcttttcacg
     4921 acctcttgat ataaacgata ttcaaaagct tgcaactgcg gtaacaagtc cagccaaatg
     4981 aaaaatataa ggagctattt ttatttgacc attcttatta cttgttgcag ggcagctttt
     5041 tcactacctt ctgcgacact tcagttggaa ttttcttccc atcctttggt cgcggtcatt
     5101 accaagataa aattatcttg caaaggagaa atagaacatg gaaaaaaata atacccgttc
     5161 agccaaacgc tggacaacga cagatgtgac tcggatggcg ctagtcactg gtttatatgt
     5221 ggcagtgacg ctggttttgt ctgtctttag ctttggagtg attcagattc gtctctcgga
     5281 gatgttcaac tatctgtcct tgtataacaa aagatacatc gtcgcggtaa cagcgggagt
     5341 agctttggct aatattgctt ctcctcttgg actgatagat gttgcagtag gaagcgtcag
     5401 cacgctgctt gtattgctta tcaactataa aatcactagt cgcatcaaaa acatgaaaat
     5461 caaaatggcc gtgacagcat gcgtatttgc tttttccatg tttacagtag ctggtcagct
     5521 aacgatttta tatcaactac cttttttctt gaattggtgg gtgattgctt taggagaatt
     5581 ggcatcaatg attgtcggtg ggataattat ttattggatc ggtaaaaaag tggatttgtc
     5641 aaaataaaga tctgtttaat ttaaaatagg gcagcatgga agagactgaa tgttgagtct
     5701 tcttccatgc tgcttttttg attaatttgt atgaagaaat cgatttagcc gttttttcat
     5761 caagataccg atataagctg ctccagttgc cttgattgct tccggcagta aaaacggcag
     5821 tagtccactg gcaaatgcag ttgtaaaagt aagatcacca cttattttca gccagatcgt
     5881 tccgcagatc aatgcagcaa gagagcccat tagattggca aagattgcat ataagtaatg
     5941 agcatccgtt ctttcgatca tccaaccagt tatccacgtt tgtaagagaa atccaatcaa
     6001 atatccgcca gttggcccaa ataaaacagc tatacctcca gtcatccctg aaaatacagg
     6061 aagaccaatc aagccaagta aaagataaac tccaacagat atcaatccta gttttcttcc
     6121 gagaaaagtt gctgttaatc caatagcaaa tgtttgccct gtcaaaggaa ccaaaccaaa
     6181 aggaatggtc atctgagaaa ggacagaaat aatcgcggca aattccgcag caagtatcaa
     6241 ttctttcaat gttcggttca taacgccctc ttttcgttaa cttaatattt ttatttggtt
     6301 aacgaaattg taaaacaaag tgattacttt gtaaatagaa aatataaaaa aagaccagct
     6361 atccaataga tagcggtctt tttcaagaaa gcttttgaaa ttataagctt tggttgtagt
     6421 attcaacgac aagagcttcg tcgatttctg gatatagttc gtcacgttct ggtaaacgag
     6481 tcaatgaacc ttccaatttt tcagcgtcaa agcttacgaa tgctgggcgt ccaacagttg
     6541 cttctacagc ttcttttact gttacgatgt tttgtgattt ttcacgaact gagatcactt
     6601 gacccacttc tacgtggtat gaagggatat caacgcgttt gccatcaact aaaatgtgac
     6661 catggttaac taattgacgt gcttgacgac gagtcgttgc aagtcctaaa cggtaaacaa
     6721 cattgtctaa acgttgttct agtaagatca tgaagttaac accatgttta ccttctttga
     6781 ttttgctagc ttttacgaac aagttacgga attgacgttc gttcattccg tacatgtgac
     6841 gaagtttttg tttttcagtt aattgcatgc cgtattctga ttgtttgcca cggctattag
     6901 gtccatgttg acctggtgcg taagggcgac gtgctaattc ttttcctgtg cctgacaatg
     6961 agatgcctaa gcgacgagaa attttccatg atggtcctgt ataacgagac attaaaattt
     7021 cctccaatga atataagttt ttggagtaaa ataatccgtt gaaaaattca taatcatata
     7081 gtttgttctt caatcttcac ctttgcagcc gcggttacac aatcgaaccc gagacggggc
     7141 aacaaactag ggatgagtta ttatttttct gctgcattat tttacacaaa ttacagtata
     7201 tgatagatca tccacgaatg tcaagataat tagtacataa aaacgcttta caaacctagg
     7261 ctgtctttat aaaatgagag ggtatgtgca aatggaaaaa agcgaaggaa gtttaaggag
     7321 aaaatcaatg aatccggtat caccattatt tgagaaaatc gatgcttcaa ttgaaaagaa
     7381 ggcgaatctt tttgaaaaca gcttttcacg ttacgctgtt cgagcggttc tcgcctgttt
     7441 gtttttgact ttaggaacgg ctgtggcatt tgcgatcgcg atgaaggggg aagatatcgc
     7501 gcatggtcta ggaaagattt tatacgcatt tatgttcagc tggggtctgg tcatgattct
     7561 atacatgaac gccgaattag gaacatcgaa tatgctttat atgaccgtgg gtgtttttcg
     7621 gcagagactt gctttaagta aggcagccaa gattctattt gcctgtattt ttttcaatat
     7681 gattggcggt atattatgtg gatttctaat ttctatgact acgccatttc aagatttaac
     7741 gttggacaac tttatgttag aatcaatagc agtaaaacta gcaaaacctt cagcaacgat
     7801 tttggtggaa gggatgtttg cgaatatcgt tgtcaatacc gctgtactga tcagcatgcg
     7861 gatgaaagag gatgtcggaa aagtgatcac agtcgtattc atcattttca tttttgcttt
     7921 tttaggatat gaacacgtga tagcgaattt tcctgccttt acgcttactt actttgcttc
     7981 tcacggacag atggatggaa tgacagtcag caatgtgtta cataatttgt tttttgcttt
     8041 ggcaggaaat tatattggtg gcggtttagt aatgggactg ggatatgctt ggctgaatca
     8101 gtcaaaatca tcctatgtgg actaaaaagt cgttcattaa aggggaagtg ccatggaagt
     8161 tttagttttt ccaaaaaatg aggaaaaagc gttacaaatg gaaaagtaca tgaaaaatct
     8221 tttttccttt gctggagtac ctaagccgga acgcgctctt ttacaacaac cgttactgga
     8281 tgagagcaaa aatttatcta tttctgaagt tttagctttt gttcattatt attatgaaca
     8341 aatcgaaaga gagtatcagt atgtagcgat cgatttagtc cagaaaaaca tcaaacggct
     8401 tacatttgaa gacttagtcg tattgttgcc actagttcag aaaaaagaat ggtgggacag
     8461 catcgactct tggagaaaag tatacagcac atggtgtaaa gcctacccag agcagcgtga
     8521 agaagtcttt caattgtttg aaaaaaagga agatttttgg ctacgtcgaa tagcaatcac
     8581 gttgcaatta ggttataaac agcaaacaga cacaggacta ttgatccgag caatagacga
     8641 agatcgtcaa acaaaggaat tctttatcca aaaagcaatt ggttgggcat tacgcgatta
     8701 tagtaagacc gatgccgatt gggtaaaaag acagctgaca acagatttga gtaagttagc
     8761 tgttcgagag gcaagtaagt atttataaga aagcgagacg acaagagatg agacgcaaat
     8821 tatttgcttt tgatattgat ggaacgttgt tagattcaaa caaacaagct ttagatagca
     8881 caagagaagc attggaaaaa ttacgcaaag ccggtcatct ggtcacgatt gctacagggc
     8941 ggagccgatt tcatgcgcaa gagatcattc gcgatcttga ttttacgaac tacattttat
     9001 gtaatggagc ggccggtttt ttagaccatg aacaagtata taagaattta ctagatcgcg
     9061 accagttgga tgcgttcgtt caagaagcgc atcatgagca gatcgataca gcttttgtca
     9121 gcttagacaa tatcaagcgt tcttcttcca atgatgtaac ggtgatggaa gaagcgatgc
     9181 attctttcgg tgctgtcttg cctgaattag atcagttttt tgcagaggaa caagaaatat
     9241 accaagcgtt agcatttttc gatcaaacat atgatgggaa gttctcttca tatgataaaa
     9301 ttcgatttgt ccgctggcat gagaacagtg tagatgtcgt acctaaaaat ggttctaaag
     9361 cagcgacaat tctgcactta gcagaacaag taggcatccc tcatgaagac atcatcagct
     9421 ttggcgatgg acaaaacgac cgtgaaatgt tgcgcatgtc aggaattggg atagctatgg
     9481 ggaatgctgt gccagaagta caagcagaag ctaagatggt cactgataca aacgatcagg
     9541 atggaatttg gaaggcattg aaagagttaa gcgtgattta aaagaggttg tgaaaaagct
     9601 gttctgcatc aagtaataag cccagccaaa caaaaatagc tcctcctatt tttcgttttt
     9661 ctgggcttat taccgcagat gcaagctttc gaacaccgtt taaggaagag gtcgtgacag
     9721 aagtggtcag tttcaagaaa taagaaggga tttacgaaaa ttgcttctca aatttttgta
     9781 aaattacggc ttatttctga agaagtagat gaaacaacgt ttattcggga aatgggactg
     9841 tgacaagatg tttgtcatag tcccattttt ttattttatt tttcaaaact aagaaatacc
     9901 aggaaagagt tccttttgtt tgaccttttc tttcttattg aaaacaacta taatatagag
     9961 tgatgttaaa agagaagagg aatttagtgt gcgggtattg tttattggtg atgtagtagg
    10021 ttcaatggga cgagagatga ttactgaata tcttcctaga ttgaagaaga agtaccgtcc
    10081 gcaagtaacg attgtaaatg gtgagaatgc cgcttctgga cgtggaatca cagaaaaaat
    10141 ctataaaaaa tttttgcaag acggcgtaga tgtcgtgacg atggggaatc atacgtggga
    10201 caaccgcgga atctttgagt ttgtaaatga agctaaaaag atggttcgtc cggcaaattt
    10261 tccggaaggg acgcctggac aagggatggt ctttgtcaaa gtgaaccaag tcgaactggc
    10321 agtgatcaac atgcaggctc gttcttttat ggtggacttg gatgatcctt ttcgtaaaat
    10381 gaatgagctg gtagaagaag ccagaaaacg tacgccgatc atttttgtag attttcatgg
    10441 agaaacaaca agtgaaaaac aagcaatggg ctggttttta gatgggaaag tttcagcagt
    10501 ggtcggtaca catacacacg ttcagacaaa tgatgcgcga attttgccaa gaggaacagc
    10561 gtatctgaca gacgttggga tgacaggtcc ctatgatggt attttgggta tgcgcagaga
    10621 acctgtaatc gaaaagtttc tgactgcatt accaaaacgt tttgaagttg tggaacaagg
    10681 ccgatcgatt ttaagcggct gtatcttgga attggatgat acgacaggtc atgcaaaaga
    10741 aatccagctg attcagatca acgatgatcg accgttcatg gagtagaaag agaggcagtt
    10801 ttttgcagca ggagccggaa attcaaaaag aagtaaggaa actgacaaaa cttttaagag
    10861 agaatgaaac gatcattcgc tacaaagaac ttgaagaaaa gatccagcaa aaccagtatt
    10921 tagctgagtt gagagaaaaa atcaaacaag cgcaaaaaga tgctgttcat tttgcccact
    10981 atgacaaacc agcagcagaa aaagaagcaa tcaaacaagc ggatcaattc atgcaagaat
    11041 ttgatcagca tccgcttgtt gtagcttata gaaaacagct gttggaagca gatgatctgc
    11101 ttcatcattt aacaactatg atacaagaag aaatcaatgg acaaatagag gaggaaaaac
    11161 atgcctcaaa aaactaaaaa tactcctatg atggaacaat atctaagcat caaggcccaa
    11221 taccaagatg cttttttgtt ctatcgttta ggagactttt atgaactatt ttacgaagat
    11281 gcgatcaaag cgtcacagct tttggaatta acgttgacga gcagaaatcg aaatgcagaa
    11341 gaacctattc ctatgtgcgg tgtacctcac catgcagcac aaggatatat cgatgccttg
    11401 attgaaaaag gctataaagt agcgatttgc gagcaggtag aagatccaaa gacgactaaa
    11461 gggatggtca agcgcgaagt gatccagcta gtcactcctg gtacagtcat ggacagcaaa
    11521 ggattgtcgg caaaagacaa caatttttta acagctgtag ttagtcaagg aaatgaattt
    11581 ggctttgctt atgccgattt aagtacggga gaattgaaga cggctcggtt gcatgatgaa
    11641 gaagctgttt taaatgaagc ctctgcattg cagacaaaag aactcgtatt aggcagtgag
    11701 atcactgata cccttcgtga acagctaagc gggcgtttag ggctagtctt ttctcaacaa
    11761 aatgagatgg aagaaaacgc ggaatttagc tttttgacaa gcgaactagt cgatccgctg
    11821 gaaaaagaag caaccggcaa actattgact tatttatcgg tgacacaaaa gcggagttta
    11881 gctcatatcc aaaaagcagt agaataccaa ccagatcatt ttttgaaaat ggattattat
    11941 tcaaaattca acctagaatt aagccaatct attcggactg ggaaaaagca cggcactctt
    12001 ctatggttgt tagatgaaac aaaaacagca atgggtggcc gtttactaaa acaatggcta
    12061 gaccgtccgc ttatccaggc aaaacaaatt aaagcaagac aagaaatggt cgcttctttg
    12121 ctagacagct attttgaacg tatcgatttg cagtcagcat taacgaaagt ctatgatctg
    12181 gaacgcctag caggtcgagt agctttcggt aatgtcaatg ggcgagactt gatccagctg
    12241 aaaacttctt tagaacaagt gccaatgatc agagggttga ttgaaggaat cgatcaaggt
    12301 caatggcaaa gtctgctttc agaaatgcag ccgatggacc atctcgtcca attgatcgac
    12361 caagctatcc aagatgatcc gccgttacaa ataacggaag gaaacatcat caaagatgga
    12421 ttcaatgagc agctcgatat gtaccgttct gctatgagaa acggaaagaa atggttggca
    12481 gaacttgaag caaaagagcg tcaagaaaca ggaatcaaga acttaaaaat cggatttaac
    12541 cgcgttttcg gttattatat agaaattaca aaagcaaatc ttggaaatgc agaactggag
    12601 aagtatgaac gaaaacaaac tttagcaaat gcagagcgtt tcatcacacc agaactaaaa
    12661 gaattggaga cacaaatcct cgaagcagaa gaaaaatctg ttgatttaga atatcagttg
    12721 tttttagctg tgagagaaga agtcaaaaag gctatccagc cgctacaagt acttgcaaaa
    12781 gcaatcagtg cagtagatgt gctacaaagt tttgcaacga tcagcgaacg ctatcagtat
    12841 gttcggccag aacttgtcag tgacaagcat cagcttctga tcaaagacgg acgacatcct
    12901 gtagtagaaa aagtattagg acatcaggaa tatatcccta acagcgtaga aatggcagaa
    12961 gacgaaatga tcttgctgat tacaggaccg aatatgtctg gtaaaagtac gtatatgcga
    13021 cagttggcat taactgttct gatggcgcaa atgggctgtt tcgttccggc aaaacaagca
    13081 gttttaccta tttttgatcg gatttttacc cgaattggtg caagtgacga cttaattgct
    13141 ggacaaagta cttttatggt agagatgatg gaagccaatc aagccctgcg ttatgctacg
    13201 ccaaacagtt tgattttgtt tgacgaacta gggcgaggaa cagcaaccta tgacggaatg
    13261 gcgttggccc aagcaatcat cgaatatatc catcgtcatg tacaagcaaa aacactcttt
    13321 tctactcatt atcatgaact gacggtactt gagaaagaac ttccgcagct taaaaatgtc
    13381 catgttggag ccgttgaaaa agacggagaa gttgtcttct tgcataaaat gatggatgga
    13441 cctgcggata aaagctacgg catacatgtg gcaaagatcg caggattgcc ttccaatcta
    13501 ctagaacgag cagcagatat cctgcatacg ctagaaaatg gcgataacgg acaccatact
    13561 gttccagctg aagtagaaga gaaaactaaa acagccaaac aggagcaaat aaaaccacct
    13621 gcagaaaaag taaatcaaga ccatatacaa gaccgtgtaa aagaagaaaa ccaacagctt
    13681 tctttattca gtgaattatc cgaaaatgaa acagaagtca ttgaatcact gaagcaaata
    13741 aatttactag aaatgacgcc aatggatgca ctaaacacat tatatgaatt acaaaaacaa
    13801 ctgtaggaag aaggcggata tcgatggcca aaatccaaga attatctgaa cgtttagcta
    13861 atcagatcgc tgcgggagaa gtagtggagc gaccagcttc cgttgtgaaa gaattagtgg
    13921 aaaatgcaat tgacgccggc agtactcaga ttgatatctt agttgaagaa gcgggactgc
    13981 ggacgatcca agtgattgat aacggagaag gcattttggc agacgacgta gagaatgcct
    14041 tcaagcggca tgcgacaagt aagatccata atcgcgacga tctttttcgc atacgaacgc
    14101 tgggctttcg aggggaagct ttgccaagta ttgcctctgt ttctgaaatg actctggaaa
    14161 cggcgaatca ggaagaaaaa caaggcacct atctgtcgct gaaaggcgga gaagttattg
    14221 agcatcgacc agcaccactg cgtcaaggaa cgaagattac cgtcagcaat ctgtttttca
    14281 atacgccagc ccgcttgaaa tacgtgaaaa cactgcacac agaattagcg aacatcgggg
    14341 acatcgtcaa tcgactggca ttgagtcatc catcgattgc ttttcgttta gtacatgatg
    14401 gaaacaaaat gctggcaact gctggaaatg gcgatctgaa acaaacaatt gcgggaatct
    14461 acggattatc taccgcaaaa aaaatgctga aaatcgaagg aaaagatcta gattttgagt
    14521 tatttggcta tgtttcattg cctgaggtga ctcgtgccag taggaattac ttatcgacga
    14581 tcatcaatgg acgttttatc aagaactttt ccttgaataa agcaatcgtt gcaggttatg
    14641 ggtcaaaatt aatggtcggt cgtttcccta ttgctgtatt ggaaatcaaa atggacccgc
    14701 ttttagtaga tgtaaatgtt catccgacga agcaggaagt ccggctgtcg aaggaaaaag
    14761 aactaacaga gctaattagt aacgccattc aaaaagcatt gtctcaagaa aatctgattc
    14821 caagtgcagc ggataatcta cggtttaaga aaaaagtacc tgatcagcct aaaacagaac
    14881 aaatcgaaat cgatttgtcc tacgcttttg aagaagaaaa acctaaaaag ccaagtagtt
    14941 tgaattttga tcgagagaca ggtacctttt tcgtagaaga actaaagaac acatttgttg
    15001 acagaattgt tgataagtct gtggaaaatg ttgaaaacta ttctggtttt tctgaagaaa
    15061 acaccactta tccagaaaaa acagcgaaaa cggtggataa gtttggtgaa aatgttgata
    15121 actttgtaga aaaaacagtg gaaaaatctg aaaagaaaga atttcccatt gtggataaca
    15181 tggagaaaac aaatgaagaa caagcagata aatccgtaga agaggatgaa aagatttacg
    15241 aggaagaatt gacactccat cctgaatttg atttcactca aggaaacgac aaaacattag
    15301 aacaagccat caagaaatta gaaacggaaa aaccagtaca acgttttcct cagttggaat
    15361 attttggaca gatgcacgga acgtatcttt ttgcccaaag tagcgatggg ttgtacatca
    15421 ttgatcagca tgctgcccaa gagcgcatta agtatgaata tttccgggaa aaaattgggg
    15481 aagtatctga tgatctgcaa gaattgcttg ttccatttgt gttagactat ccaaacagtg
    15541 atgctttgaa attaaaagag caaaaacact tgctagaaga tgcagggatt tatttagaag
    15601 aatttggtca aaacagcttt attgtacggg cccatcctac ttggtatccc aatggagaag
    15661 aagaatcgat tattcgggag atgatcgata tgctgctgac aactggaagt gtgagtgtaa
    15721 aaaaattccg tgaagcaaca gcaatcatga tgagttgcaa acgatcgatt aaagccaacc
    15781 attatttgaa tgaagcacaa gcacgtgtgc tgctgaagga tctggcacga tgcgaaaatc
    15841 ctttcaattg tccccatggc cgtccagtat tgattcactt taccaattcg gatatggaaa
    15901 ggatgttcaa acgaatccaa gacccgcatt aagtcaaaga gaagagggaa aacatgaaga
    15961 ttgtattagc atcccaatca ccaagaagaa aagagttatt ggctcatatc gtttcagaat
    16021 tcgagattga acctgctgat atcgatgaaa cgccatttcc agacgaacag cccgtcgatt
    16081 atgttcgtag aatggctgaa gaaaaagcac gaactgtctg ggaacaagat gaaaaagaag
    16141 accatttagt gattgccagt gacacgactg ttgttctcaa tcaagagatc atgggtaaac
    16201 cagaagatct ggctgaggct gaattcatgc tgaaaaaact aagcggggaa acacatactg
    16261 tatatacagc tgttgtttta aaaacaaagg aacgagaaga acggatacta gcagaagcac
    16321 atgtcacttt ttatcctttg acagacgaag agatcaaacg ctacttggag acgggggatt
    16381 atgcggataa ggcaggggca tacggcatcc aaaatgctgc gagtgtattc gtcaaagaaa
    16441 tttctggaga ttattacagt atcgtaggat ttccgattgg tgctgtcaat caggcactga
    16501 aaacattcat ttaaacaaaa aagcggacta tctgattttc gattgaaaat ccatgatagc
    16561 ccgttttttt tattgaaaag attacttatc tactctgact aagattttac ttcagcttta
    16621 ccgactgctt cgcccgggcg aacaagtcct gattggtcaa gagaaagtcc tgcaactttt
    16681 tcattattgg tcaaagcaac aataatgtca gttttcttgc cagcttgttc tatttctggc
    16741 aaatccattt cagcaatcag ggtctctggt gtgacaaggt atccttcttt cacgaataca
    16801 ttgaaaggaa caccctttag ttcaacagta tctaatccca tgtgaacaag tacttccagc
    16861 ccctcattag aaagaatacc aatggcatgt tttgtttcaa agacactcgt cactttaccg
    16921 tttactggtg catagacttt gccgtttgac ggttcgacag cataaccgtc acccatcatt
    16981 ttttgtgaga aaactgggtc atttacttca gttatttcct tgatggtccc tgcagcgact
    17041 gcgaataatg tttcttttgt tgcaactgcc gtaccagttg ctgaaacagg ttcgaaagac
    17101 tgtggcgttg ctgttgcgcc atttgctgtt tgaagttgta catcagcagc attttcagga
    17161 acagcaggat caatcttatt caaaattccc tgacgtctaa agatgatcgt caagccaaat
    17221 ggaatgatga cagcaatgat cattgcgatg atgaaaggta cccatgtttc tgcttggata
    17281 gctagtattc ctggcaaacc gcctactccg atcgcgttag ctcgaacacc catcaagtta
    17341 gcaaacatgc cagctaatcc agagccgacc attgcagcga cgaaaggata gacgtatttc
    17401 aaattgattc cgaacatagc aggttcagtt acgcctaagt aacaagaaat cattgctgga
    17461 atcgagattt gttcttcttt tttattccca cgatgtaaga agatgatggc taagactgca
    17521 gagccttggg cgatatttga taaagcaatc atcggccata aattcgttga tccgaaatct
    17581 gcaatcagct gcatatcgat ggcattggtc atatggtgga gaccagtaat cactaaagga
    17641 gcatacagga atccgaatac ggcactaaac aaccagctga tagaagacgt caatccagta
    17701 tttacgatat ttgagatcca gcttccgact gtccagccga taggacctaa aataacgtgt
    17761 gcagctaaaa cagttggtaa caatgaaaac aatggaacaa aaatcatcga gatcgactga
    17821 gggatatatt tgcggaagaa gatttctaga tacgctaaca taaaccctgc cagcatggct
    17881 ggaataactt gcgcttgata accaatcatc tggacttgag caaaaccaaa atcccaaaat
    17941 ggaatatctg ctgcggctgt ggaagctaca ctataagcat ttaataattg cggagaaact
    18001 aacgtaattc ctaaaacgat tcctaatatt tgtgtagtac ccatcttttt tgcaatactc
    18061 caagtgatac ctactggaag aaaatggaaa attgcttcac cgggtaacca caaaaaggca
    18121 ttgacaccat tccaaaactt cgagtgttca acgattgttc caccaagact ttcaaactgg
    18181 ataccttcaa gtacatttct gaatccaagg atcaatccac cgacaatgat tgcaggaatc
    18241 agtggtgtaa agatttctgc taaaacggca atagcacgtt gaacgggatg aaggttttgt
    18301 tttgcagctg cttttccttg ttctttggaa accccttcaa ctccagatac tgcaacgaag
    18361 tcgttataaa aagtagaaac atcgtttcct atgatgactt ggaactgtcc tgcattcgtg
    18421 aacatccctt ttacgctagg tatatcttcg atcgcttttt cgtccgcttt tcctggatca
    18481 ttcaggacaa aacgcatacg agtcgcacaa tgactaactg cagcaatatt ttcttttccg
    18541 ccgatatcat gtaacagctt ctcggcatct gcttgatatt ttgccatatc aataaacccc
    18601 tttctcttaa cttgtctatg caagttgttt cttcatttac tatacttttt ttcggagaga
    18661 attgcaagcg ttttattaaa aagtacgaga gaaaatgata tcgttttaaa aagcttaaaa
    18721 taagcttttc ttaaaaatag gaacattgaa atacaaaaag agctgtagta tagtgaagaa
    18781 atgatggaag aacgagtgag ggaaaaaaca tgaataagtt caatgaaatt tttttagatt
    18841 tggaacagaa aattttagcg aaagaatatc ctccgcattc gcttttaccc agtgagaacc
    18901 aattgatcaa aatttatggt gtttcacgag aaacaattag aaaagcgctg aatcttttga
    18961 ctacggaagg ttatattcaa aaaaaacaag gaaaaggttc gatcgtactg gatcggaatc
    19021 gcttcgactt tcctatttct ggattgacta gctataaaga gctacaggag acccaacgta
    19081 ttccaagtga aacgatcatc catacgttag aagaaacaga agtgacaaaa gcattgaacg
    19141 agatcactgg ctgggagatc ggtgcgcctg tttggcattt gatacgcgaa agaaaaatca
    19201 atggcgaagt agttatatta gacaccgatt atctattaaa ggaaatcgtt cctattttaa
    19261 ctccagcaca agcacaagga tcgatctatg aatattttga aaatgagctt tctttgacga
    19321 ttgcttacgc gcaaaaggag atcacagtag aagaagtaac ggaaatcatg aaaacagcga
    19381 tggatcttca cgatgacact tatgcagtag tagtcagaag cctcgttcat ctggaagata
    19441 cccgctgttt cgagtattct gagtcggttc accgattaga taaattccgt ttcgttgagt
    19501 ttgcccgacg ccgaaaagtg taaatagttc aaaagaaagc aaattgttgg catctgtcca
    19561 ctttggcgct atgataggat cacaaagggg gcatttttaa tggataaacc aataagacca
    19621 gaaaaagaag aactgaaaga aaagttatca cccatggctt atgcagtcac acaagaaaat
    19681 gcaacagaac gaccatttag cgggaaatat gatgattttt acgaaaaggg gatctatgta
    19741 gatatcgtca gcggtgaacc gttattcagc tctgcagaaa aatatgatgc aggttgtggc
    19801 tggccatcat tcagcaaacc aataacgaaa agacaagtgc gcgaaaaagc cgatttttct
    19861 catggcatgc atcgaataga agttcgaagc aaagaagcag attctcatct cggacatgtg
    19921 tttaccgatg gaccagtaga tcaaggtggt ctacgttact gcatcaattc tgcctcactg
    19981 aaattcattc catatgaaaa gatggatgaa ttaggttatg gggaattcaa atcacttgta
    20041 gaataatgga aatacacaaa gagcggtaca atacggggat ttactcgatc gtccgctctt
    20101 tttttattca agcgattgct atttgaaaga aacgctccct agactccttt tttcacaagg
    20161 tgaaatgtgc tacaataaag ggaacgtatg tgtgcaaaag aaagaggact agaatatgta
    20221 tgaatatctg acaggtgtgg ttacctttat caacccctat tacatagtag tagaaaccaa
    20281 tggtatcggc taccagatcg cactaggaaa tccttaccgc tacagtagca aactaaatca
    20341 aaaaataaaa atttatgtcc atcaggtaat acgtgaagat gcgcatcttc tgtatggatt
    20401 tgattcgcta gaagaaaagc aactgttttt gcgattagta agtgtttcag gaatcggtcc
    20461 gaaaagtgcc cttgcaatca tggcaagcga agaccactcc ggtttgattc aagcagtaga
    20521 atccagtgat gtcacttatt tgacgaaatt tccaggagta gggaaaaaga cagcacaaca
    20581 aatgatcttg gatctaaaag gaaaattcgg tgagctgagt atcgatacac cttttagtct
    20641 atttgatgaa gcaaacacaa aagacgctac agctctatct gaagcaatgg aagctctctc
    20701 ggctcttggt tacagtgatc gagaaatcaa acgtgtagaa aaacaattaa aagaagtaga
    20761 taatcagaca acggatgagt atctacgcca agcactcaag ctaatgatga aaaaataagg
    20821 aggagaccaa atgacagatg aatacttgtt atcaccagaa tctggcgaaa atgaactgag
    20881 tttggaaaaa tctcttcgac cacagctgct ttctcaatat atcggacaga gaaaagtcaa
    20941 acaagaactt cagatttaca tcgaagctgc cagaaatcgg gaagaacctt tagatcatgt
    21001 tctgttgtac gggcctcctg gattaggaaa aacgacgatg gccatggtca ttgctaatga
    21061 gatgaccgta aatattcgta cgacgagtgg ccctgctatc gaacgtccgg gtgacttagt
    21121 agcaatcttg aatgaactgg aagcagggga tgtcctgttt atcgatgaga tccatcgatt
    21181 acctagagtg gtagaagaga tgctttattc agctatggaa gatttctatg tagatatcat
    21241 ggtaggacaa gggccaaccg cacatccggt tcatttccct cttcctccct ttactttgat
    21301 tggcgcgacg acacgggcag gtatgctttc tgcaccttta cgagatcgat ttggcatcat
    21361 ttctcatatg gaatattatg acgaacagga tctgcaggag atcgttttac gttcttcgga
    21421 tatttttcaa acagagatca ttgaagaagg ggcattggaa attgctcgcc gatcacgagg
    21481 aacgcctaga atcgctaatc gcttgctcaa acgggttcgt gattttgcgc aagtccaagg
    21541 agacgggaaa atcgatcgtc ctatagcgga tcaggcactt actttgctac aggtcgatca
    21601 ggctggattg gattatgttg accagaaatt gttgcgaaca atgatcgagt tatatggcgg
    21661 cggaccagta gggttaagta cgctttcagt aaacatagga gaagagcgtg agacggtaga
    21721 agatatgtat gaaccgtatc tgatccaaaa aggattttta aagcgtacac ctagagggcg
    21781 aattgctact gcttatgcct atgaacattt tggttacgac tatcatatat gatttttgct
    21841 tttagattgg aggaaaatga atgagtgata caacacgtac gaaacaattg atcaaaaacg
    21901 tattgattga tttgtgccaa acgcaatctt ttcgaaaaat cagcgtccaa aaaatcgcaa
    21961 acgaagcaaa aatcaatcga caaacatttt attatcattt tacagataaa tacgatttgc
    22021 ttcgttgggt ctattgtgag gatgcattgc attatttaga tccacaatgt ctttcacttg
    22081 ataattggga agagcaagca ttgaaaatgc tgaaagcaat tgctgagaag aaagaatttt
    22141 atgcaaatac tgtgctttct gacccagaaa ttttacaaaa agagttttct caactgatcc
    22201 aaaaatcatt tatcaagatt tttgaacaaa tggatgaaga aagacaactt tctgcaagag
    22261 acaaactatt ttatgctcgt tttttttctt acggttgcag tggtgtatta gtcaattgga
    22321 tcacgaatga atatgaagaa acaccgatgg aaatcgctat gcagcttttc cgtttagcca
    22381 aggatacgga attatattct tatcgtcttt atgcaaaaga agagttggaa gaacagtaat
    22441 agaaaaattt tttgttggaa aagcaataat cgctaaaagg agatggggaa atgacaaaaa
    22501 ttttatttgt ttgtttggga aatatttgcc gttcaccgat ggcagaaggc ttgctgagaa
    22561 aacgaatagc attagaagga agggaagcag aattttttgt ggattctgct gctacaagca
    22621 cgtatgaagt tggaaagaca ccgcaccctg gaacgaaaaa aatattaaac caagaaaaag
    22681 tagatatgac gaatatggtt gctcggcaaa tcacacccca cgattttgaa acttttgatt
    22741 ggatcattgg catggatcaa gaaaatgtag aagaactgaa acgaagagca ccacgctcag
    22801 cgcaggggaa aatccatttg tttttaagta gcattcctgg aaaagaaaaa gaaaatgttc
    22861 ccgacccata ctataccaat aactttgatg agacatatca cttgatcaca gaaggcctga
    22921 atcattggtg gaatatttgg attgatgaaa ataaaataac aaacaatttg taaagttaac
    22981 gctttctttt taaggcgatg ttagagcatt tccttgtttt tttgtaaaat gttaggtaaa
    23041 ataaaattaa ttaagtaaca ctttcttagc taaaggagga gtgcattcga tgaacgaaga
    23101 gacagtatcg ccaatgaaga agcttacgaa gttagttact catacttcgt atcaactttt
    23161 tgattctttt gtaaatgaca ctgaagaaca tgctaagaat gtacgattgc aaggaaagtt
    23221 tattcttgag ctgcaaaaag ctgctactga acatagtcta gttatcttac aagtaagagc
    23281 aaatcgtttg agtagcaagt atgaaacgat ctcaggctgg gtggcgacaa aaacggtcga
    23341 aaaagaccga gtgatgatcc gcctacaaaa cgaagagcag cagatccgga tggttcctgt
    23401 agaacaaatt gaaaaaataa caatcctttc accagcaggg gaacaggaac ggttatcaaa
    23461 ataatttata ccagatagaa aagcacgtat gagcttgtac catcaatcat tactaatcaa
    23521 aaataaccga atgctttaag ctgatatcag taaaacggaa cgttttagga tgagtatcac
    23581 agcatgacag cattgggttg attagttgaa tcgattggat ggtacaagtt tttttgataa
    23641 aaaaaggtcc tgctagccgg ggagctagca ggataaggag ttaaaaatga aaaagtgttg
    23701 ttagggttgt ttgttggtat gcttatataa tagtcactag ttgtgaatat tttgtgacga
    23761 aagttttcga aagttgttaa gtttttggga gcgtcaataa ttttgtgtaa ataaattgtc
    23821 ctcctgcaaa ataattagtt actcagtaaa cattgaaact aatgtatcgg ttacctgttg
    23881 aaaaccttta tggcttctgt ttagaaattt ttgattgtat gtatcaaaaa tgctgactag
    23941 aaagcgttct agtgattctt cattttgaaa ctgctctttt ctacggctgt atcttttaat
    24001 ttgcttattg aaagactcga ttagattggt tgagtaaatg gttctacgaa tgctaggtgg
    24061 aaaatcataa aaagttaata agtcttggtt ttctatgagt gactgcgtca ctttaggata
    24121 gtttttcttc catttctcaa tcatgccgga taagaaggta ttcgcttctt cttttgagtt
    24181 agcttgataa acagccttaa agtcatcaca gatttctttt cggtcttt
//
LOCUS       DAZKOB010000033        24096 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000033 DAZKOB010000000
VERSION     DAZKOB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.033"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..433)
                     /locus_tag="ACG8M6_001280"
     CDS             complement(65..433)
                     /locus_tag="ACG8M6_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0456960.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            604..1560
                     /locus_tag="ACG8M6_001281"
     CDS             604..1560
                     /locus_tag="ACG8M6_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0456961.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1633..1749
                     /locus_tag="ACG8M6_001282"
     CDS             1633..1749
                     /locus_tag="ACG8M6_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0456962.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1804..3486)
                     /gene="recN"
                     /locus_tag="ACG8M6_001283"
     CDS             complement(1804..3486)
                     /gene="recN"
                     /locus_tag="ACG8M6_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0456963.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3504..3953)
                     /locus_tag="ACG8M6_001284"
     CDS             complement(3504..3953)
                     /locus_tag="ACG8M6_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0456964.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(4060..4878)
                     /locus_tag="ACG8M6_001285"
     CDS             complement(4060..4878)
                     /locus_tag="ACG8M6_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0456965.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4887..5777)
                     /locus_tag="ACG8M6_001286"
     CDS             complement(4887..5777)
                     /locus_tag="ACG8M6_001286"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0456966.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5779..6003)
                     /locus_tag="ACG8M6_001287"
     CDS             complement(5779..6003)
                     /locus_tag="ACG8M6_001287"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0456967.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(6008..7345)
                     /gene="xseA"
                     /locus_tag="ACG8M6_001288"
     CDS             complement(6008..7345)
                     /gene="xseA"
                     /locus_tag="ACG8M6_001288"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0456968.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(7346..8200)
                     /locus_tag="ACG8M6_001289"
     CDS             complement(7346..8200)
                     /locus_tag="ACG8M6_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0456969.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(8297..8746)
                     /gene="nusB"
                     /locus_tag="ACG8M6_001290"
     CDS             complement(8297..8746)
                     /gene="nusB"
                     /locus_tag="ACG8M6_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0456970.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8739..9164)
                     /locus_tag="ACG8M6_001291"
     CDS             complement(8739..9164)
                     /locus_tag="ACG8M6_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0456971.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(9410..10474)
                     /locus_tag="ACG8M6_001292"
     CDS             complement(9410..10474)
                     /locus_tag="ACG8M6_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0456972.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10646..10891)
                     /locus_tag="ACG8M6_001293"
     CDS             complement(10646..10891)
                     /locus_tag="ACG8M6_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0456973.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(11030..11404)
                     /locus_tag="ACG8M6_001294"
     CDS             complement(11030..11404)
                     /locus_tag="ACG8M6_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0456974.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(11487..12401)
                     /locus_tag="ACG8M6_001295"
     CDS             complement(11487..12401)
                     /locus_tag="ACG8M6_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0456975.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(12422..13225)
                     /locus_tag="ACG8M6_001296"
     CDS             complement(12422..13225)
                     /locus_tag="ACG8M6_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0456976.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(13258..14247)
                     /locus_tag="ACG8M6_001297"
     CDS             complement(13258..14247)
                     /locus_tag="ACG8M6_001297"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0456977.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(14350..14841)
                     /locus_tag="ACG8M6_001298"
     CDS             complement(14350..14841)
                     /locus_tag="ACG8M6_001298"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0456978.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(15032..17839)
                     /locus_tag="ACG8M6_001299"
     CDS             complement(15032..17839)
                     /locus_tag="ACG8M6_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0456979.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(18273..18566)
                     /gene="rpmA"
                     /locus_tag="ACG8M6_001300"
     CDS             complement(18273..18566)
                     /gene="rpmA"
                     /locus_tag="ACG8M6_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0456980.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18579..18923)
                     /locus_tag="ACG8M6_001301"
     CDS             complement(18579..18923)
                     /locus_tag="ACG8M6_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0456981.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18950..19258)
                     /gene="rplU"
                     /locus_tag="ACG8M6_001302"
     CDS             complement(18950..19258)
                     /gene="rplU"
                     /locus_tag="ACG8M6_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0456982.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(19278..19354)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            19540..20181
                     /locus_tag="ACG8M6_001303"
     CDS             19540..20181
                     /locus_tag="ACG8M6_001303"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0456983.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(20243..21874)
                     /locus_tag="ACG8M6_001304"
     CDS             complement(20243..21874)
                     /locus_tag="ACG8M6_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0456984.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(21997..22356)
                     /locus_tag="ACG8M6_001305"
     CDS             complement(21997..22356)
                     /locus_tag="ACG8M6_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0456985.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(22515..22588)
                     /locus_tag="ACG8M6_001306"
     tRNA            complement(22515..22588)
                     /locus_tag="ACG8M6_001306"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22552..22554),aa:Arg,seq:tct)
     gene            complement(22897..23580)
                     /locus_tag="ACG8M6_001307"
     CDS             complement(22897..23580)
                     /locus_tag="ACG8M6_001307"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0456986.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(23708..>24096)
                     /locus_tag="ACG8M6_001308"
     CDS             complement(23708..>24096)
                     /locus_tag="ACG8M6_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HFQ0456987.1"
                     /translation="SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAV
                     ENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIA
                     FCLTGITAWNTFVELKKKRRRTQKFL"
BASE COUNT         7035 a         5278 c         3988 g         7795 t
ORIGIN      
        1 atttttttaa aaatcaagct gcaaaaagcc ttttaaaaga ctttttacag cttttctatt
       61 tttttcatag gtaagaaagt atgttcacaa tgatcaagac agcgtacaag acgattgtca
      121 acagaaaaac caaacgccaa gtcatcttga aataacgcgg ataaataatt tcttcataat
      181 aatatgcttg gaaaacagca atacaaattc ccattagcaa aacagaaata aagaaaaacg
      241 gtaagataga aattgtccct gtatttctag acaatgcatg cataccgccc cacaaaaaag
      301 gaatcgccaa atcaggggct tttattttaa aacgacgatt taacgaaaat aattttacaa
      361 gaaattggca accaaacaaa acgattaccg ggtaaatata ccaaaacaag ataagtggtg
      421 tgaatgtgcc catgaacgta aactcctttt ttatcgttgc ttcaagtata ctgtatatac
      481 tttacacaac tgtttcattt tatttaaatt tatgcagtta tattctatat cttacaaaca
      541 cattccatgt attccaattc cctatccgtt ataataaaag aaacaggcaa caggaggaaa
      601 atcatgatca aatattttac attagataat gagaaactgc gtaataccgg aaaggaaact
      661 gaccaaacga tctggtattg tgtagaacgt cctaatcaag aagagattca tcagctgaca
      721 catcaatttg aaattccgtc agattacatc acttccatat tggacgatgc agaaaattcc
      781 cgtacagaag aattcaatca atcaaagttg acaaagccag cgttattatt gttacaatat
      841 ccttttgcta aaacaagtcc tagcggttat tttcaagtgg atacataccc gctctctatt
      901 attttgacgc ccaaaaagaa gcttatcaca attacgaatc atgaaccact gtttttaaag
      961 aaagtcttta gccagacctt tccaaaaagt gatatttcgc ctaatctcaa tatcttcttt
     1021 caattcattt ggcaggtcac actctcttac aacagttatt tagctacgct tcaatctcaa
     1081 tgcgatactt tggaagccaa acttcaagta tccactgaaa acaaacaact ttatcaaatc
     1141 atggatatcc aaaaaagcct cgtttatttc agagaagcga cttctgctaa tttagatgca
     1201 ttgaaagaac tagcgcaaaa taaacttttc caagaaaatc atgctttgaa aaatcattta
     1261 cgtgatgtat tggtggaaac agagcaggca atgactactt ctcgcattca attaaaacta
     1321 gtagaacaaa tgaatcagac tttttcagca atcgtatcga ataatctgaa taatattatg
     1381 aaaattttaa cttctttaac gattatttta actataccga caatcgtcgg cagtatctat
     1441 ggaatgaaca ttaagttacc tattgctgaa agggatgatg cctttatatg gctagttcta
     1501 ttaacattat ttatttgtat cgtaactacc tattatttaa aaaaagggaa gtttttataa
     1561 aaaagccctc ttgtggaaat ggaaaaaaga ttgtacactt gaatcaacta attaatgacg
     1621 aggttgaaaa caatgcgtga aaaaaaacaa acaagtactt ttgctaaagt aacaaaattt
     1681 gtcatttgga caatgctgat tttaacgatt ggatctgttg tattgactgc tgtaatgagt
     1741 gtcatgtaaa aataaaatat agctatcagc tgctgaaaag gacctgaacc actgcgaggt
     1801 ggctcaggtc ctttttgcga gtttcaatag ttcttttgca tgttcgagtg tgagttttgt
     1861 aatctcactt ccagccagca tacgtgcaat ttcttcttct ctttcttttg ttttcaactc
     1921 tgaaacagaa gtttgcgtac gtccatcaat cactgctttc acaatataat attgatagtc
     1981 ggcgactgca gcaacttgag gcagatgagt gatgcacaaa acttgagagt atttcgatat
     2041 ttgagaaatt ttatctgcga tggcctgtgc aactcttccg ctcacacctg tatccacttc
     2101 atcaaaaata atacttgtca caccttgctc agaagaaaaa atagttttca aagcaagcaa
     2161 catccgagac agttcgccac cagaagcgac tttcaccaaa ggcttcagtg gctctcctgg
     2221 attggtcgta atgtaaaatt cgatttggtc aaatccatca tgattcaatt gtctattttc
     2281 ttcttttaag aaacgtacct caaactcagt gttttccata tacagacttt tcaattcatg
     2341 cagaatggat ttttccaatt cttttgcgat ttttttacgc gcttcatgaa gatctgcggc
     2401 ttgttgctga agtagaagct ctttttgctc tagttcctct ttcattttgt ccaattgacc
     2461 ttctgaaaaa tcagaagaag ctagctcttc ggtgatttcg tcatagtaag ccaatattga
     2521 ctcaatggat tcgccgtatt tacgtttcaa ctgtcggatt aattctaaac gttgcgtaac
     2581 ttcttctaaa cgactctcat ctaactccaa taaatcaatc tgacgactca tatcgccgat
     2641 tgcatcttgc agcaaatagt aagcactttg gatattttca taaattgctt catactccac
     2701 atctagatgg gcgatccctt gcagttctga aacggccaag ccaacaccat ctacactgtt
     2761 ttgttcatta ttcgtcagtg ctccgtaacc tgcagctaat ccatcagcaa ttttttgata
     2821 attgctaagt ttttctcttt cttcttttaa tttctcttct tctccgactt gcaaatcagc
     2881 agcagctatt tcttcttgct ggaaatggag catatctatt ctctgaacat aggatttttc
     2941 attttgctgc acttttcgta cccttttttc cagctcacga taagagatat attcttgctg
     3001 ataactcttt ttcttctgtt ggaaagctac atctccaaaa cgatccaata aagcaagatg
     3061 tgattcaggc tggagcagtt cctgatgttc gttttgtcct tggatatcta ctaaataact
     3121 gccgattctt ctcagattag ctaatgtgat aatatgacca ttcactcgac aaacattttt
     3181 tccagttaag gacatatccc gtcgaacaat cagattgtcc tcatctgttt cgatccctaa
     3241 ttcaaccatc agttcagaga atccttcttg ttttggtagt tcaaacaacc cttcaagcac
     3301 acatttttct gctccttgac gtatataatc gcttgaacct cttcctcctg ctaacagacc
     3361 taaagcatcg ataataatgg atttacccgc accagtctct ccggtcagcg cggtcattcc
     3421 ttcgtgaaaa gaaagccgca attccggaat aatcgcaaag ttagtaatac tgatttcttg
     3481 tagcatcaca ttcgctcctc aacttaaagg taatgcagga agtctttctt caataaaact
     3541 gcctggtctt cagttcgtgt aatcatcaaa acattatcgt catcattgat aatcgaaaat
     3601 aattctttct tgtaacgttt atcaatcaaa tttgctgctg ccgaagcatt tcccggcaat
     3661 gttttcaaaa taacaaattt ttccatttgg tcaactgcca cgaaagcgtc ttttaatagt
     3721 ttttccaatt tagtatttac atcttcgttt gtttcagctg gtatgctata acggtagccg
     3781 ccatctgaag ccggtacctt gatcagtttc agttccttta tatctctgga aatcgtagct
     3841 tgagtaacag atattccttt gcttttcagg atttcaacaa attcttcctg ctttcggata
     3901 tcttgctcac tcaacaatcg agtaatcaaa cgatgccgat ctttttttct cattacgtct
     3961 tcgctcctta gcatattctt cttaatcaat gatagcttat ttccttggta taataaagaa
     4021 gaatccgcat acttctcact tgattttcac agttacaatt tatttatggt caaactgttc
     4081 gtgtgcattt gaaaccactt ctgccatatt aatagctgaa ttgattgttc cgctttcggg
     4141 tactttcctc aagtgagcca agaactcgat atttccttct ccccccgtga tcggagaata
     4201 acttaattcc agcacatcat atccaccttg tgccgcaaat gacaaaatgt cttctaaaac
     4261 tttttgatgg gtctgagggt cacgaacaat tccgtttttc cctacagctt ctctgcctgc
     4321 ttcgaattgg ggtttgatta aagcaatcac ttctccctct tcttttaaaa tagcatgtaa
     4381 tggaggcagc atcagtttta gtgaaataaa agaaacatcg ataacagcaa cttctggaat
     4441 tcctaaggta aagtcttcgg gttttgcata acgaaaattc actctttcca ttacgacgac
     4501 tctaggatct tgtcttattt tccaagctag ctgattgtag ccaacgtcta aagcataact
     4561 catttttgcg ccattttgca aagctgcatc agtaaatccg ccagtggatg caccaatatc
     4621 taacaatgtt tttccttcaa cagatagatc aaatgctttg agtgcttttt ctaattttaa
     4681 tccccctcga gaaacataag gaagtttttt acctttgact cttaattcac tactcactgg
     4741 aattttttct cccggtttat caaaccgctc gtttttttcg ttgtaaatca aaccagccat
     4801 cactgaacgt ttggcttgtt ctcttgtttc aaataatcct tgtttgaccg ctaatacatc
     4861 tacgcgttct ttttccattc tttccctcat ttctccacat caaattgctt gatcatctgg
     4921 cgcatcagcc cgccatcaaa agaaaaaacg tcctcttcta atttgtctat gatcttgttg
     4981 gcggataaca gctcaatctc caacgcctca cgtgtctttt ctaggcctaa taaacgcggg
     5041 taagtatttt tttccatacg ttcatcttta ccagctgttt tccctaatgt tttcgttgta
     5101 ccaatcacat cgagtagatc atctcttatc tgaaaagcta aacctaaatg ttcggctaat
     5161 cgttgaagta gcaataagat ttcttcaggt tgcttcgcta atatgccacc agccagcaag
     5221 gcataacgaa tcaaatgccc tgtctttctt tcatgaataa atgctaattc ttctaaagaa
     5281 agctttttcg attcgccctc gatatctgca gcttgcccag caaccatacc ttgtgatccc
     5341 gcacaaacag ctaattgctg aagcagcaga agcttaggag aattacctaa gtgtgccata
     5401 ctgattagct ggaatgctcc tgtaagcaaa ccatctccgg ccaatatagc taatgcttcg
     5461 ccgaatactt tatgattggt aggttttcct ctacgaagat catcattgtc catcgcaggc
     5521 aaatcatcgt ggatcaagga atacgtatgc accatttcta aagcagctgc tacttgatag
     5581 acgggaacat caataggctc atcaaaggaa gcaacggccg ttaaaagtaa taagggacgt
     5641 attcttttcc cgcctgcttt cactgaataa ctcatcgcat catagagtcc tctatccgtt
     5701 gtatgctcat ctaaaaaaga caagatttct ttttctacag caggtagatg aatagctgaa
     5761 aagtcagtta atctcatctc agctttcctc gtttccgtca aaatcaactt cttcatttga
     5821 ttctgtcatc attttcgtca aggtctcttc tgctttagct aatgtatctt ggcaatgttt
     5881 gcttaattcc attcctcgtt taaacgaatc tagagctgat tctaatggaa cgtctccttg
     5941 ttctaactgc atgacaattt tttctaattc ttgcagtgac tcttcaaaag ttggattttc
     6001 catattttta ttctcctttg tctatctctt gtacttgtcc tttaacgatt ccatcttgat
     6061 aatggatcgt cagcaaatcc ccttgatgaa tctcggtgac actttttact acttgatcat
     6121 ttaacgtcgt atagctgtag cctcttccca tgattttcaa tggactcaac aaatcaagag
     6181 attgaacagc ttgagcaaaa cgctgctgct gttgtttcat atattgttgg attttctctt
     6241 ctaagcgttt ttctagataa gcagtttctt ggcgagctgc tttaactcga taaagcggag
     6301 ccacttgttc caattggtgg cttaatgcga tcgtttgttt ttctttcgca tgataaattt
     6361 gctgtgtagc ttgatataat cgctgtttca actggtctaa tttgatggtc tgaccttcat
     6421 acaatctttc aggctgccga aatacatagg aacgtcgagc acgctcgaac cgttcttgtt
     6481 tgcgctggat ctggcgtaca aacgcctgct gcaatctagc ttgccgttct tgaatgcgca
     6541 tcaactcttc tgacaaaaca ggaacagcta attctgcagc tgctgttgga gtggcagccc
     6601 gaacatctgc tactaaatca gcgatcgtgg tatctgtttc atgtcctaca gatgaaatca
     6661 ccggcgtatt agcttgataa atcgcccgag caacccgctc ttcattaaac ggccataaat
     6721 cctcaatcga accaccccca cgaccgatga tcattgtatc gaaattgccc atctcttcta
     6781 ctctctggat attcctgaca acattgtccg ctgcttggtt tccttgtaca actgttggga
     6841 acaaaaccaa ttggacaatt ggatatcttc ttttcaccgt cgtgatgata tcgcggataa
     6901 ccgctccgct tggactggta aggaccgcga ttcgctttgg gaaacgtggc agcatttttt
     6961 taggcccttg aaaaaggcct tccttcatca gcttttcttt tcgttcttcc aattcttgat
     7021 ataaagcacc tattccatca ggctccatgt gctctacata gatttgataa gaacctcccg
     7081 cttcatataa tgaaatacgc ccaacgacaa ggactttcat tccttctttt ggctggaatc
     7141 gtaatttttg gaatgctcct ttgaacataa tagcagatat ttttgcatgg tcatctttta
     7201 agctgaaata ttgatgggca ttggcacgta aacggaaatt cgatatttcg cctgttaaat
     7261 atacgcgttc taaataagga tcggcatcaa attttctttt caaatacttc gttaatgtag
     7321 tgacagttaa atattcttgt gccatctatt cacctgctcc aaaagcttcg gcagatttta
     7381 cggtttgata catcagcatc gtgatagtca ttggccctac accctttggc acaggagtga
     7441 tccagcctgc aataggctcc acttcatcaa atttcacatc accgatcagc ttaccgttag
     7501 catcacgatt catccctaca tctatcacta cagcacctgg ttttacgaat tcttttgtga
     7561 caaaatgtcc acgtcctatt gctacgacta aaatatctgc ttcttgtgcc agttcagcca
     7621 gattttcggt tttagaatga gcaacagtaa cagttgcatc tgccatcaag agtagctgag
     7681 ccatcggttt accgacgata ttgcttcgtc cgataactac tgctcgttta cccgccggat
     7741 cgatagaata agcttcaaac attttcatga tgccgtatgg agtacaagga atcttgtccg
     7801 gtttacccgc aaggagtctg cctaaattca tcggatgaaa accgtctacg tctttttcag
     7861 ggcggatagc taacaggatc ttttcttcat ctatatgctt tggcaatggt aattggacca
     7921 atataccatg aaaactagga tcttgattgt atttttcaat ttctgcaagt agttcgtctt
     7981 cagaaatcgt ctcaggataa cgctctacca atgaatggat tccgatttct tttgctacac
     8041 gttctttatt tcttacatag atttggctgg ctggattttc ccctaccaat aaaacgacta
     8101 atccgggatg gataccatta tctttcaatt tttggatttt ttcagcaatc tctgcctgca
     8161 ttttttcagc taattctttt ccgttaatca attctgccat ctaaccactc ccagatattt
     8221 tttgtgtttt cattctacca tagaattttc ccgatttata acaggtttat gatgattcat
     8281 agaaaaaagc taggacttac gccccagctt ctaattcttt caatacattg gataaaacac
     8341 cgttgacgaa tttacgtgaa cggtcatcac taaatgtttt tgccaactcg attgcttcat
     8401 ttaaagcaac agccgctggt acatctgtca cataagtcat ttcaaaaatc gcgatacgca
     8461 agatcaccaa atccattttt gagatccgat tgatgtgcca attgtttttt aaatgttttt
     8521 caatcacttt atccaattca tctttttttg cacagacacc gccaactagc gtatcaagat
     8581 aaacaggaac aaactctgat tcatcttcgt tgatcatgtc gcggtggtct aattcgattg
     8641 cgttgaaaat cgcatcttct tttgttaggt ctgcattgaa gtctaatgga aataaagcct
     8701 gcaatgccat ttcccgaatc tcgtgtcttg ataattcttt attcattctc ttcctcttcg
     8761 tcttcaaaaa gttcttcgat tgttggttgt tctaattttt caggaacaac agctactaca
     8821 tggatattga cttcagataa atcgatatcc gtcatgaaaa ggacttgctg gctgacccgt
     8881 tcctgcattt ccattgctac tttaggaact gaaacaccat attctagata acagtaaaga
     8941 tctactttca agccatcttc ctcgtttgct aagtaaacac cttttccgtg agctgcacgt
     9001 cctaataatt cagtgacatt gctggcaaaa gttccgcgca ttccgtatac gccttctact
     9061 ttagaagctg caatcccgat aattacttcg atgacttcag gagcgatcac gatctctcct
     9121 aaatcttggt ttgcgtttaa aatcaaattt ttttcatctg ccatttattt attcctccct
     9181 ttgcctaagc acgagataca tccgaattct ctatgatgcg gcatagtgat cacgtggtaa
     9241 gaaaactgcc gccgatcagc tgcaaactgc tatgaattgg agaaaacacc caactttatt
     9301 atctcttaca gcttaaaagt tcttctatag tgtatcatta aatcgagtga ttttctataa
     9361 caaagtctgt gaacactgat ttttacgctt acttcttcta ttgatgaaac tataagatga
     9421 tcaattcttt tggtgaacga gtcaacacac gatttccatc ttctgtgatc aacaagtcat
     9481 cctcaatacg tactccaccg attcctggta agtagatccc tggttcatca gtgatcacat
     9541 ttcctggtac aaattgcttt tctgcacgag aagaaacgtt tggtccttcg tggatctcta
     9601 aaccgatccc atgtcctgta gaatgtccaa atgcttcccc gtaaccatgt tttgaaatat
     9661 aatctctagc aactgcatct aattggacac cagtcattcc tggttttgca gcatcaataa
     9721 ctgctaattg agcatctaag actatttgat aaatttcttt taatttttcc ccaggatcac
     9781 caatagcgaa tgtacgtgtc atatcggaaa cgtacccttc gtaatagcaa ccaaagtcca
     9841 atgtgatcaa gtcaccttgt tcgatgattt ttttgcttgc tacaccatgc ggcatagccg
     9901 aacgcaatcc gctagcaacg atcgtatcaa aagaaacacc tgaagcgcca agagaacgca
     9961 tataaaagtc tagttgattt gcgacttcaa tctcagtcat gcccggttgg atcattttca
    10021 ggatgtgatc atatgccata tccgcaatat gacatgcttt ttcgataata gcgatttctt
    10081 cttcatcttt cacttctctg agttcttcga tcatctgtga aacaggtact aattccgcat
    10141 cgatgatttc ttctaaaacc gaatattcta aaaatgagac tgttgtttcc tcaaaagcta
    10201 atgagtgcag ttcttctttg cgtactaagt ttgctacctc atcaaaaata ggtccgacat
    10261 ttttaataat ttcaaaccct tgagcctgcg ctgccgcctg ctcggtataa cgaaaatcag
    10321 taataaaaaa tgctttttcc aaagtaataa cagctaatcc tgtcgtacct gtaaagttcg
    10381 ttaagtaacg aagattgtac ggactggtaa ccaaaaatga atccaaattt tcttcttgca
    10441 tttttttgcg caatttttct acacgtagca tcataatgaa actccctctt tctttgcacg
    10501 cttttttcca ttatagcaaa cttttcttcg tacacgacag ttaaataaaa atttccggaa
    10561 aacttctttt tattttaaat aaataaagcc aaaaaggagt cacagtctcg ctgcaactcc
    10621 ttttagttta ctattttgta aaaacttatg atcgttttcc aaagaaaaat gagacaactg
    10681 ctaccaaaat cactgcgcct aaaatagatg gaataagtgc catacctgca gcttttggtc
    10741 cccattcgcc gaaaatcgct tgtccaatac ttgaaccgac tagtccagca atgatattcg
    10801 ttacacagcc catcgaattt cctttactgg tgatcgcacc tgcaattgct ccgatcacgg
    10861 ctccaacaat caatgtccat aaccaactca taatataacc tccttctttg ctaatcaatt
    10921 ttagcatatg ggatttaaag gggacaaata attggttttg ctatttcata ttttcttctg
    10981 caaagtaaaa aaataaagaa aacctcctct tagaagtttt ctttattttt tacttgcgtt
    11041 taaacatact acgaatacca cgtttcacta catcaaagaa actcaatgat tgtaccattc
    11101 tatcttcatc tacatattct ctgatcgcac gaagcacttt tttaggttct ttggaagaaa
    11161 atgtgtatgt accatttttt ttcgtttgaa tggcatatcg aggaatccat tttcccttta
    11221 gcatgactga tgctagtaca taatccactt cttcccatgg aatttggata tatttgcgtc
    11281 tatctcgcgc atggtaaaac tcaaaaccat tattacctat catgatttgc ccgtaatctg
    11341 tcagacccgt aaacgaagta gcatcaatta ccaagtctac ttttgtattg attgattgaa
    11401 ccattttctc cctccagata tttgtttatg aacagtatac tatataaaaa aagctcggga
    11461 caacgcccga gctatatttt tatggattat aaaagtccga tgacgtgtcc aacaacaccg
    11521 actacgaata aaccaaggat gataacgatt ggagaaacct ttttcttaag caaccacata
    11581 cataacaatg ttaatcctaa tgcagctaaa ccaggaatca attggtctaa gttgtcttgt
    11641 aatgttgtta ccttttcagg agaaagggct aagccattat taacttgttc aaatgcactc
    11701 ttgattcctt caccacctga aggcaattta tcccattcga tgtaagcacc tttatctaat
    11761 tttacagttg atacaactgg taagaatttg attgataccc atctttgtac aagggcagct
    11821 aatacgaaca tccctaaaat tgaagcacct tttgttacgt cttgtaacaa tccgccagaa
    11881 aggtcatcag taattttaga accagctttg tagccaaatt cttgtgtata ccacatgaat
    11941 gcccaacgaa taatgttcca acctaagaag aagatgattg gtccaaggat atttcctccc
    12001 atagctaatg aagcacctag tgcacctagc attggacgta ctgtaaacca gaacactggg
    12061 tcaccaacac cagctaaagg acccatcata cctactttaa ccccttgaat cgctacatca
    12121 tcaacaggcg cgccattcgc acgttcttct tccagagcca atgtaacccc taaaattgga
    12181 gaagcaatgt atgggtgtgt attaaagaat tccaagtgac gttttaatgc tgcagaacgt
    12241 tcttcttttg ttttgtataa ttttttgatt gctggaatca tagaaaatgc ccagccaccg
    12301 ttttgcatac gttcgtagtt ccatgaacct tgaatgaagg tagagcgcca cgcaacagct
    12361 aaacgatctt tttttgataa tttgattttt tcttctgcca ttttattttc ctccttcaag
    12421 attaatagtc attcaaaatg tcgcctaaag ggtcaccaga gttgcttccg cctccattat
    12481 ttgatgaacc acccatttta gaaaggttca agtaaataag agctaatgca acacctaaag
    12541 cacctagagc aatcaatgtt aattgagaaa ttgcagctac aacgaaacca ataacgaaga
    12601 atggccatac ttcttttgta gccatcatgt tgattaccaa agcgtaacct acagctacga
    12661 ccattccccc accgatagcc ataccatctg ttaaccatgc tggcattgat tcaaggaatg
    12721 attgcacagt ttgtgctggg ataaatagca acgctgctgc tgggaccgcg atacgaaccc
    12781 cttgtaaaca tacagcaatg atgtgccaca tttcaacctt acggatgttt ccttcttcag
    12841 cagccgcatc catcaaatga acgattggaa cagcgattgt acgtacgatc attgttaaga
    12901 acaaccctgc aacagccaaa ggaacggcaa tcgcgatcgc agaaggaaca cctttaacac
    12961 cttgtccacc taaaactaaa ataattgctg atgctactga tgctaatgca gcatctggtg
    13021 ctacagcggc accgatattt gcccaaccta aagcgatcat ctgaagcgta ccgccaagta
    13081 cgataccagc ttcgaggtta ccggtaacta aaccgatcaa tgtacatgcc actagtggtt
    13141 gatgaaattg aaattcatca agaatacctt ccataccagc tagaaaggca actaatacga
    13201 ctaaaattat tgaaataata gacatgataa gcctcctata aattttaata ttatttatta
    13261 cgcttttgct aattcagctt ttgctttttt caaaatatca tccatgttat cgcgtgaatc
    13321 attaggtact ttacgtacat cgaatttcac acctttttgt tcaagcttct caaacgcttc
    13381 aacgtcttct tgtcccatag acaatacttt acttacaacg actttaccga ccgaatgcgc
    13441 cattgaccca acatttaatt ctttgatgtc tacgccacct tcaacagctg tcaatacgtc
    13501 ttcaggattt tcaaataaaa gcaatgcttt ggtatttcca aaacgagggt cttttgctac
    13561 ttcgatcatt ttgctaatag gaataacatt tgcttttaca cctggaggag cagcttgttc
    13621 gattagtctc ttacgcagat catcctttga tactgcatct gaaacaacga taatacggtt
    13681 tggttgcact gcttttgtcc aagctgtcgc aacttgtcca tgtaacagac gtgagtcaac
    13741 acgtgctaat acatatttga tttttccgtc tccgaccaca gttccttctg gaagtgctcc
    13801 tttaggttgt gcgtcttcaa ctgctgcttt cggtgcttct gccggttcta attcttccgg
    13861 ttttactttt acaccgtctt ttgctgtttc aatgatgtgt gctgcaattt catgagcaga
    13921 ttccattgaa aagcgtgatg cgtaggcttc aatcaacatt ggtaaattca aaccgctgac
    13981 gattgcccat ttatctttgt gctcttcgaa taatgtgttt gattgattga acggtgttcc
    14041 gccccataaa tcaactaaga ataacacttc gtcttgatta tcaaaagaag caaccgcttc
    14101 ttccaatttg gctctcaagt catctggacc ttcacttggt ttcaaaatca cggctttgac
    14161 gttttcttgt tcgccaaaga tcattgaacc ggattgtaag attccttcag caaattgtcc
    14221 atgacttgct aggataatcc ctaccatatt tatacctcct atttactttt gttgtaacaa
    14281 attcaaaaat attaataatt gctaaccatt acaacagaat tatttgacct tttccaacaa
    14341 ccattcttgc tacacctttt tgattttcgc tagtagttcc atcaaaagga ttttcctgtc
    14401 agcaggtact tttcgaatct ctaactccac ttcttgactg ttcaagtaac gaaaggaatc
    14461 aatatcttgt tgatttactg aaacaaaatt ggtaatcatt gatttgcctt cagtaaaact
    14521 cataccgccg atattcaacg acttcaactg tacaccgcag cggacaaccg cttcaacatc
    14581 ttggggattg gtgaataaaa gcatcacttt tgctcgtgat aataatccac tatgatagat
    14641 ttgaatcatt ttctgcacgg ttattacatt tgctgtgatc cccggcggtg cagcttcttt
    14701 tagtagaaac tttctcaatt tatcttgagc gactacatcg ctgatcacaa tgatacgttc
    14761 aacacctgtt tgcttcgacc aaactgttgc gacttgcccg tggattaaac gatcatctat
    14821 acgtgccaat cgaatatcca tctgcaaaac tccttcttcc tcactacttc acagaaaccc
    14881 ctatcatttc tgtgtgaaca accacctcct agcacacact actataagca aaaagcgtgc
    14941 caactttttt acgatacact aattgaattt aaaggattct taaccaatac acaaaaacga
    15001 tacactataa aagtgtatcg tttttgtgta ttcatggcac agtgtattct tttttatcta
    15061 actgattatg gactagacgt agcagataat acatttcttc tatcggcact tttactttta
    15121 tctgatcttc caacagaaca actgaggctg ccaattggct ataaagagga tcattcgttg
    15181 tatttgtcaa ttcgttttct tccacagtca atggttcatt ccgaataata cgttcgatca
    15241 ttcctgctgt gtgcatcact aggttgattt tgaatccata ttcttcatcg ccgattcccg
    15301 tcgtctgaga gatttgtgta gaaaattgcc acattggatc gattaactta ctaccattaa
    15361 taaagatgaa gttatcagag ataaactcaa cacacgtatt tttagctgac tcttcatcta
    15421 gggagatgaa ttcagtttcc tccaattctg attccatcag caactgatcc aaaatgactt
    15481 cgatattttg atcaataaaa cgttctaaag gaataaatgg ggcttggata ttcaaatcag
    15541 taatcccagt agtagctatg atctgatatt ctttttggat aactggcagt tcttctttca
    15601 cttccacaat cgacaaagct agaacttcta attctgtttc ttgacgcttg tttaccgctc
    15661 gttcgatcaa ttctttgatt ctttgtgctg taccttcgcc agatgcgcag atagccaaaa
    15721 cagctttttt cttatgaata ggttttgctt cctgaacaga ttctacagaa gatatttctg
    15781 cgtaacctcg aaaatttttc aatgattcat gcaacatatc tagatctgtc cctaatactg
    15841 agacctttcg tacagtttcc aaaactagag aagttgttac catatcaaca gtacgtactg
    15901 gaattccagt gtctctttga atttgcgaat taaacgacgc aagggacccc atatctacta
    15961 agagtatgac cccgcttcct tcatctactt cttgtacatt gcgttcaatt cggtccaatg
    16021 ctgttttcgg atccatatcc aaaggcatgt ctactgctcg tacgttctct gcatctaaca
    16081 actgttggac aacttggacc atactgcttg ctgtgctgtt tccgtgagct gcaacgacta
    16141 caccgattct gccgcttgct tgatttgccc gtaacgagac gagtagaacg gtcaagtaat
    16201 agtcttcact ttctggaatc tccacttgaa aatacgtttc gatataacta cgcacttctt
    16261 tcgcaacagc atattcatct ggaaaatcaa aagccatctc acggatgtta tcattcgtat
    16321 gccgttcttc tcctaagttg atttttttca aaaaagaact gatatgcaag ctcatagcat
    16381 aaataaagtt ttgctgaaac tcatatggta aagatctttt gaccattaca taaatctgat
    16441 tggtcacttc tatgattttc ggatctacaa attctgctaa tttgctgtct gcattaaaag
    16501 aaaaaccatg gtctttatag aaagacttca gatggatgtt gatatctgta gaaatgaaat
    16561 gattgattgc ttcttgatcc aaaccatccg acttcagtaa agctgcttta tctccgatga
    16621 tatcatatag attataaggt aactcatagg agtccgattg gattttcatg atcgtatcat
    16681 ttggtgctac agttatcttt ggctccaata ttttagaaag ctctgccatt gcactgcggt
    16741 tgcttgctaa ttgaatcaat ccgctacgaa tcccttctgt caaatcatcg attgtgatcg
    16801 agatttcggg tgattgcata tgatttagaa atcctcgtgc acatactaac tggatatttg
    16861 acttcaattg tcctatattt ccataggtca cactgccgat caatgctttt gctacatctt
    16921 ctgtcaaaga aatctttcgc tgcgtgcggt ttgcttcgtg ggcaatcatg atttttacta
    16981 aatcgacttt ctcatttgca ggacgttttt caaaagccgg aagtttgata ttgattggga
    17041 tacgccgaac aaaagtttct aatagagaag aacctgggtc ttctgtggtc gcacctacga
    17101 tccgtacatt tgcttcatgg gatttcgttg tttccccaag acgactgtaa gttccatgat
    17161 ccataaagta aaagatcatt tcttgacctt caggcggcaa ccggtgaatc tcatccaaaa
    17221 acagcatacc gccatccgct tgatcgataa tccccatttt ttcttcatct gcgcctgtaa
    17281 aagcaccttt tgcgtaccca aataaatgac tcatcaaaag ctcagggtta ttggcataat
    17341 cagcacaatt gaaaacaatc agttccgttt cctcatctat tacatgatgc gcttttgcaa
    17401 aatgatacat cgcgtgtgca aaataagtct ttcctgatcc ggtaggtcct gtaatcaaac
    17461 aatttaatcc tcttggcgga tacaaaatcg cagcttttgc ctgttccact gaatttttca
    17521 tgctgccgtt agctccaatg atttttgcaa agatatcctt agtatctatt tggagtgtat
    17581 ttttttcttc agagaccggc gtttctttgt aactgactac atgttttgta actggcttta
    17641 cgtgcgtaac gagtgtttga tatatgtaac gtactggtcg tccgtctgtc tttatcagtt
    17701 ttccttcgcg cactagctga ttcagatctt tactgctatt tgttcgctga atccctaaca
    17761 cttcagcaat ttcctgtgtt gttactcctt gatcatattc tgcctttgtt aaatgagcgg
    17821 ttttttcttt gacatacata taaattcggt caatgcgttt tgccaatggt atcgctcctt
    17881 ttctatagac tattgtactt aggcttcaaa acataatact atgtatttat ttctgtttcg
    17941 tcaactattt acctgatttt gttttgttga taacacgcgg atagatagaa atgttcaatt
    18001 taaaagaacg aaaaatcccg atacgatttt tataatggaa acaagaaaaa gaacactgtt
    18061 taccatataa ttatttcact gatagcaaac aatatagctt ccatcaattg aaaacgatag
    18121 ttatcaaaac atcctgattc caaattggca aaaaaatatt aagtgtaaaa agacaaagta
    18181 aacttcgtag cctccactac aacttttatt tttataaaca aaaaaggctg tgacaaaagt
    18241 cttgtcacaa cctctttaat cataagattc aattaagctt cttgagctac tggataaaca
    18301 gacacttgtt ttttgttacg gcctttacgt tcgaaacgta ctacgccatc aacttttgca
    18361 aataaagtgt cgtctccacc gatacccacg tttacacctg gatagatttt tgtaccgcgt
    18421 tgacggtaaa gaattgatcc acctgtaact gtttgtccat cagcacgttt agcacctaga
    18481 cgttttgatt ctgagtcacg gccgttagat gtagaaccgc cacctttttt gtgggcaaat
    18541 aattgtagat tcatagataa caacatggtc tgcacctcct atttatcatt aattgtttta
    18601 atttggatat attctgagtt ttcagcttct atcgattgta atcctaataa aagattttct
    18661 aatagaatct cagcaatatt gttttgttct tgtgtcattc ctgaagtgac ttcggtataa
    18721 agataaccgc cttcttcttc attcatttca acaatcggct caaaaccggc taatgaggca
    18781 atcccgttga cagtgctgat cgctaacgca gaaacacccg cacaaacgat atcacttcca
    18841 taaggaccag catcagcatg cccggtcaaa gtgaatgaaa cgatctgacc tgcgtcattt
    18901 cgcttaaatg ttcctttgat catatatagg ccttctttct caaacgctat taagcgttga
    18961 tcgcgtcgat aaccactttt gtatatggtt gacgatgacc ttgtttacgg tgtgaatgtt
    19021 ttttaggttt gtatttgaaa gtaacgactt tcttttgttt gccgtgtttt tcaacagtac
    19081 cttcaacagt tgcacctgca acagttggag cgcctacttt tgtagattct ccgcctacta
    19141 agataacttc gtcaaaaaca actttttctc ctgcttcaac gtcaagtttt tcaacataga
    19201 ttgcttgtcc cacttcaact tttacttgtt taccaccagt tttaataatt gcgtacatgc
    19261 tataaagcac ctccttctga tatacttaga ctcgccatcc gaagtgacag catatgctgt
    19321 gcttattact tgttctgagc ggttgtagct gtggagtcca caattacaac agtactagct
    19381 taccagaaca gcaaccgtta gtcaactaaa tttaaaatca aaaagtaaaa caaaaattca
    19441 agcaatactt ctgcttttct atcctcacag ttctaccaaa taaaatagtc gcattgtgtt
    19501 tcttttattc ttataataaa agaaaaagga ggagcataaa tggaataccc aaaagaactg
    19561 atacaagaag cttctttaag aatgaacggc cgtcctgtag aaggatttat cgctgggcaa
    19621 ggacctcttc atcctaaact gatgctcgtg ggagaagccc cagggaaaac agagattgat
    19681 acgcatgttc cttttagcgg tcaagctgga aaagaattga tgcaggcact ttcttcaact
    19741 ggcttaacac gagaagaggt gtatatcacc agtgctgtaa ggagccgccc gtatcgcgtg
    19801 actcatcgga tcaataaacg gacacaacag tcagaaactg tttatccaaa tcggacacct
    19861 actcgttccg aagtatttgc gcatgcacca atattggatt acgaactgat gaaggtccag
    19921 ccaaaattaa ttgcgacact tggaaacatt ggtcttcaac gtttacttgg caaagaatat
    19981 acggttacca aaaatcatgg tcaactctat acagggcctg tcttacaatt aacagaacaa
    20041 aaggatgcct acgaatggtc agaaaaaaac tatcgaattc ttccactttt tcatccagca
    20101 gctattttct acaatcgctc ccttgtacct gttatccatc aagactggca agtactagct
    20161 tcacttttac agcaatatta aaaagaacag acaagaccat aaaatcttca gtttacagtc
    20221 ttgtctgttc tttttcattg ttttaatggc taattgcagt atatttcgca tgtaaagctg
    20281 tcaacgcatg agcaactgtt tgatgttgat gcgtgaatgt atgttcccac aagtgagaaa
    20341 gttgtttttc tacttctggc tgcaaggaag tatagataat cgtttgtcct ctttgctctg
    20401 ctttttcttg aaattcatca aaaactgcac gtgccgtcaa atcaatactt ggtacgcctc
    20461 gcatggagaa aatgacacca tcgtgatgat ttagttcttc caatttattt ttgagttttt
    20521 ctgaagacat aaaaaacagt ggaccactaa tatacactac tgtccagtta ttcagctttt
    20581 ccgtttctgg cagccccatt ctttgccaat cgatcgtttc taatgaaatc gtgatagcag
    20641 cgcttttgac aacaaaaaat acacagccgc cgatcacccc gatcacgata gcaatactta
    20701 aatcaaagat aactgtacaa atcatcgtaa ggataaacaa tagaactgct gaccaatatt
    20761 tattagtaaa tagctccttg atcgtttccc attcattcat acgccaagcc gtcacaatca
    20821 aaacacctgc aagtgctggc attggaatct gtgacatcac tggcgcaaaa acaagcatgg
    20881 agagcagtaa gacaactgca tgtatcatac cagctaatct agtttgtgca ccagatttga
    20941 tggccacgct tgtacgggcg atggccgcag tagcaggaat cccgccaaag aaaggaagca
    21001 acagatttcc aattccttgc gccattaatt cttgattgct atctaagggc ttatttgcca
    21061 ttcggccagc cgaagcacca catagcaagc tttcaatcat tcctaataaa gcaatgctaa
    21121 tagctggaac tgccacttgt tgcaaggcag aaaagctaaa atctcccata ttcaaacgat
    21181 tactactgat caatgtttgt ggaatcttac ccacagttgc tactggtaaa tggaacaata
    21241 gcataactgc tgttgccaaa atgatagcca gcagcgaact aggcattcgt ttcgcccatt
    21301 tttttggata gataaacatc cctaaaatga ctagccctcc catgatcaaa gaggctgtgt
    21361 ttatcggaaa tcctatcgtt tgataactac ttagtttttc aataacgttt gtaccttccg
    21421 agtgaacacc aaataaatta tcgatctgac ctaatgcaat aatgatagca atacctgaag
    21481 taaaccccgt aattacgggt gctgggataa aagaagtcag tgttcctaat cggaaaagtc
    21541 ctgctgcaag taaaaataga cccgctaaaa aagtagcaag caatacacct tgcattccct
    21601 ttgttgccgc aatagacatc aagatagctg ccatcgcacc tgtcggacct gatatttgat
    21661 aaaaaccgcc tgataaaccg ccaataacga taccggcaat gatagccgta atcaaacctg
    21721 cggccgcatc agctccgctt gataccccaa aagccaaggc taaaggcaat gctacagcag
    21781 ctactgtgat cccagccaaa agatctttct gaaatcttga aagatcataa cctgaaaatt
    21841 ctttttttag tgaggaaaca taatttttga acacctatct aactcctttt tcatgaaatt
    21901 ttctaaaata ccattcatta gaatatcata tttcatgaga taaagtaata tttttcacaa
    21961 agtttcctgt tagataaatt cttatttttt ctatatttat aagaaaatag aaagcaatgc
    22021 caacagagct agcccacctt gtttgagtaa aatccaacga tcgctagtca aactgccata
    22081 taatgcaaca aaaaaaatag atattaatag catacttgtg atttctttag atgcggaaga
    22141 aagataagct ccataaagta gtcccactcc taatagtcca ttgtacactc cttggttctt
    22201 aaacaatgtt tgaacagatt ttctgcttaa ctcttcttta ctcattttaa aaaccctact
    22261 tgtcgtgtca gaatcagttc tcaccgtttc gatatacatg atataaaaaa attcaattgc
    22321 aactagagtc actaaaattc tagatagtat cgtcatatct tcttcctctt ttctgatgag
    22381 ttttattttt ttgctcctta taattgtact cgataaggat gtttttttat tttatttgcc
    22441 tttgaaaaca aacaatattg gcacaaaaaa caaagaaaaa ccgttagaaa cctcagtttc
    22501 taacggtttt ttagcgtcac aggagggatt cgaacccccg accgtccgct tagaaggcgg
    22561 atgctctatc cagctgagct actatgacaa gaatcagcgg aacatcaata attatagata
    22621 attaaattgc ttctgtcaat acgaaatagt tatttaatta gcaaataatg aattattttg
    22681 gcccaactac taaatcaatc ttttaaaacg agacattctc cttcagctgt tctaaggtta
    22741 tcaattgaaa acaggagcta cattgacttc atgctactcc tgtttattta gatttcctgt
    22801 atcctttaat tgacttacaa atatagaaac ttttacatca agttcgaatt gagccctcga
    22861 ttcaatcgat tatctacagg ctgccataat catgtatcac aactcgattg tttctgattc
    22921 ctttgtcatt aagatcacac catcacctaa tggcaagatt gtcgatgtca aatcgggatg
    22981 atccatcaca acacgaagaa attcatttaa tttacgatgg atagctcgtt tgccacgggg
    23041 aatctcttca tctgggagca agatcgttcc tccttggaag atgtcatcta ccatcaaaac
    23101 gccacctttt cttaacaaac gtaagcattc aggtaaaaat tcaatatatt ttgattttgc
    23161 actatccata aaaataaagt catacggacc agacaactcc ggcaagatat ctgctgcttg
    23221 accttcaagc aaagtcactt tatctgtcag tcctagacgt tcatagtttt ttttagcttt
    23281 ctcgatcatg acatcgaaac gatcaattgt cgtaacatga gcgttttctc cgattgtcgt
    23341 ggccatgaca cttgatgaaa aaccgatagc tgctccgatc tccaagattc gttcaggctg
    23401 aagctgtcca agtaaaaatt tcaaaaatac aactgtttca tggggaataa ctggtacttc
    23461 tgctacatga gcttcttctt ccagtttgcc tagctcaccc ggtaattttt tttgctgggt
    23521 acgcataaaa tccaatagtt cttctttgac tacaggacga tgcatcattt cattgcgcat
    23581 actactcctc ctcttcacac attatacgaa caactgacta aatctacaag ctgcatgagt
    23641 gttttctcgt gtttcatgtt cagaagagtc gttggatctc tggaatacat ttatgttttt
    23701 aacattctta caaaaatttc tgtgttcgtc tccgcttctt tttcaactct acaaaggtat
    23761 tccatgctgt gattcctgtt aggcaaaaag caatcgtaaa gaataacccc tgaatccatg
    23821 caaatgccgt ttttgaaagg actagtccaa tatatggaat ctgaaagatg ggactaccga
    23881 taatatttct cttttttact actccaggct caatataagt ctttgtatca gcttttgtat
    23941 aaaaatcttc gtggttgttt tcaacggcta ttacgcgacg cgtcgagatt ttttttcctg
    24001 aattttcata atatgtaata atatctccga ctaaaatctc atttgtattt ttcggtcgta
    24061 catatatcag atttccttgt gcataatatg gactca
//
LOCUS       DAZKOB010000034        23986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000034 DAZKOB010000000
VERSION     DAZKOB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.034"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            293..457
                     /locus_tag="ACG8M6_001309"
     CDS             293..457
                     /locus_tag="ACG8M6_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456988.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            454..684
                     /locus_tag="ACG8M6_001310"
     CDS             454..684
                     /locus_tag="ACG8M6_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0456989.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            869..1414
                     /locus_tag="ACG8M6_001311"
     CDS             869..1414
                     /locus_tag="ACG8M6_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0456990.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1511..1732
                     /locus_tag="ACG8M6_001312"
     CDS             1511..1732
                     /locus_tag="ACG8M6_001312"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0456991.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1889..2053)
                     /locus_tag="ACG8M6_001313"
     CDS             complement(1889..2053)
                     /locus_tag="ACG8M6_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFQ0456992.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2155..2676
                     /locus_tag="ACG8M6_001314"
     CDS             2155..2676
                     /locus_tag="ACG8M6_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0456993.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2663..3103
                     /locus_tag="ACG8M6_001315"
                     /pseudo
     CDS             2663..3103
                     /locus_tag="ACG8M6_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3143..3301)
                     /locus_tag="ACG8M6_001316"
     CDS             complement(3143..3301)
                     /locus_tag="ACG8M6_001316"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFQ0456994.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3394..4155
                     /locus_tag="ACG8M6_001317"
     CDS             3394..4155
                     /locus_tag="ACG8M6_001317"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0456995.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            4342..5694
                     /locus_tag="ACG8M6_001318"
                     /pseudo
     CDS             4342..5694
                     /locus_tag="ACG8M6_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            5710..6930
                     /locus_tag="ACG8M6_001319"
     CDS             5710..6930
                     /locus_tag="ACG8M6_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0456996.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            6965..7642
                     /locus_tag="ACG8M6_001320"
     CDS             6965..7642
                     /locus_tag="ACG8M6_001320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0456997.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7639..8832
                     /locus_tag="ACG8M6_001321"
     CDS             7639..8832
                     /locus_tag="ACG8M6_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0456998.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8847..9173
                     /locus_tag="ACG8M6_001322"
     CDS             8847..9173
                     /locus_tag="ACG8M6_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0456999.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9177..9512
                     /locus_tag="ACG8M6_001323"
     CDS             9177..9512
                     /locus_tag="ACG8M6_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0457000.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9509..9904
                     /locus_tag="ACG8M6_001324"
     CDS             9509..9904
                     /locus_tag="ACG8M6_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0457001.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9901..10272
                     /locus_tag="ACG8M6_001325"
     CDS             9901..10272
                     /locus_tag="ACG8M6_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457002.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            10288..10848
                     /locus_tag="ACG8M6_001326"
     CDS             10288..10848
                     /locus_tag="ACG8M6_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0457003.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            10973..11284
                     /locus_tag="ACG8M6_001327"
     CDS             10973..11284
                     /locus_tag="ACG8M6_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457004.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            11308..11499
                     /locus_tag="ACG8M6_001328"
     CDS             11308..11499
                     /locus_tag="ACG8M6_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457005.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11499..15347
                     /locus_tag="ACG8M6_001329"
     CDS             11499..15347
                     /locus_tag="ACG8M6_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0457006.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            15348..16220
                     /locus_tag="ACG8M6_001330"
     CDS             15348..16220
                     /locus_tag="ACG8M6_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFQ0457007.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16217..17266
                     /locus_tag="ACG8M6_001331"
     CDS             16217..17266
                     /locus_tag="ACG8M6_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457008.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            17270..18094
                     /locus_tag="ACG8M6_001332"
     CDS             17270..18094
                     /locus_tag="ACG8M6_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457009.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18112..20172
                     /locus_tag="ACG8M6_001333"
     CDS             18112..20172
                     /locus_tag="ACG8M6_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0457010.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            20189..20338
                     /locus_tag="ACG8M6_001334"
     CDS             20189..20338
                     /locus_tag="ACG8M6_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457011.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            20335..20781
                     /locus_tag="ACG8M6_001335"
     CDS             20335..20781
                     /locus_tag="ACG8M6_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457012.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20783..20920
                     /locus_tag="ACG8M6_001336"
     CDS             20783..20920
                     /locus_tag="ACG8M6_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0457013.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            20955..21197
                     /locus_tag="ACG8M6_001337"
     CDS             20955..21197
                     /locus_tag="ACG8M6_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFQ0457014.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21213..21410
                     /locus_tag="ACG8M6_001338"
     CDS             21213..21410
                     /locus_tag="ACG8M6_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0457015.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21421..22437
                     /locus_tag="ACG8M6_001339"
     CDS             21421..22437
                     /locus_tag="ACG8M6_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0457016.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            22552..23760
                     /locus_tag="ACG8M6_001340"
     CDS             22552..23760
                     /locus_tag="ACG8M6_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457017.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            23744..23914
                     /locus_tag="ACG8M6_001341"
     CDS             23744..23914
                     /locus_tag="ACG8M6_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457018.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT         8370 a         4033 c         5260 g         6323 t
ORIGIN      
        1 atgattggat ttaaaaatgg gcaaaataaa agatagtagt aactaagaaa gaaattttta
       61 tattgtcact gtggcggaaa gggtagacgc taagcatgtg tgctaggtca atgcttcggc
      121 aactatgcaa tgttcgattc attgccagtg actttggcaa ccgaggcatc ggcggtttaa
      181 aaatataggg gtgcgcaatt tcgtacgcgt tttgtgcatc gtgtaggttg ctattacata
      241 ttagatcact cgttgagtgg tctttttatt ttacataaag gaggtaacaa cgatgtatag
      301 accacaatac ttagaacaga agtatgaagt aatcactgtg caaaacggta acggtgagat
      361 agtacgaaag tatagaagac caataaagag cgatacatat aaacgaaagg aaaacaatga
      421 agttatttct tttcgcagaa ggagaaaagc caaatgagaa actactggta tgtatcgcta
      481 actaatgaat atcctcgaac cattgatgat tgttcagtgc gtgttgtgcg ttctgtacaa
      541 atcaaaggga agtactctat tgtcgaaatg ctaagagaag ctacaccaaa cgaagtggat
      601 aaatgcaagc tgatatattg cggtcatggc tattggaaag acgagtatat ccaacaaaac
      661 attgagaggt ggatagatag atgagttacc tcgaacattt gaaacgttgc tacatgcatt
      721 ctaagaataa acttcctgac agctacactg tagatgatgt tgctatacat gtattgaaga
      781 ctgagagcca tagcagtcct gatggtagca gtaaggagca gacgctcgcg tggttcaagt
      841 tctttaagtg gattaaggag gaagagtaat gcacatgcac ataggagaaa agatatataa
      901 attgagacgg cttaataacg aaacaatgaa cgacttagtt aaacaaactg gagtgtcaaa
      961 atcatggtta tctgatattg agtctggaaa aaagcaacgc gttgatttta ataagatatg
     1021 tgcaatagcc aaacattata aagttagctt agaatacttt cgggatgatt atttggatag
     1081 tgatgaacac gaggatatta ttcctttgac aggcgaaaca agagttaaat cagtgaaatc
     1141 gcttgaacaa agcctaaagt atctgaataa caaacaagaa cctaaccttc tagagatcaa
     1201 actaaaagat actgactcag taccagaggt ttggtacaag ggtgagaggt tggatgaatt
     1261 gcctaaagga ttagtagatg tctcgtatca ttggaagact gatgatttta ctaatgatga
     1321 tagaggagcg aacgacatca cgattcaata cttttctagc tttaatgata agtatccaga
     1381 taagaaaaca atcggacaca agagagatat gtaaatgaaa gaagttagac ctagagacga
     1441 aatagacaaa ctatacaaga ccaaacgatg gcgagaccta aggcaagtag taatagctag
     1501 ggacttcggc atgtgccaag agtgcaagcg tcgagggcgg aacacaaggg gcacgatcat
     1561 ccatcacata gtcgaggcga gggaagacct gtcactgttc tggtccgtag ataaccttga
     1621 atgtatctgt gtagcttgtc acaacagaga gcatccagag agatcaggcg ggaagaagaa
     1681 accaaaacct aaatcacata tcgttaaaat gtattcaact cctgaaagat aagtttgcag
     1741 cgaaatgaag gtagcccccc tactctaaaa gattaaagag taaggcttga gaagaacggt
     1801 gctgtccttc cttcgtaaaa agaccgcttt tcaagttttt tggagaaaaa ggaaaaagcc
     1861 gaccaattta agccggcttt ggacgaagct atttcttagt ccatttgttt cctttttgag
     1921 aagtaggggg taatcggtcg cctggatcaa tttttacttc ttgtccgcct tggacatttc
     1981 caccacgagg acccacttct ttgtaggttc cttttggttt gttgtcttcg ccgggtttat
     2041 agagttctcc cataggaatc cctccttaac aaatttcggc acagcactgc cgataaccta
     2101 attataagga ttgtgataac gatttcaatc tatcttttga aagaaggtga tattatgccg
     2161 caaccagcga agagtgcaaa attacaatta ttaaacggaa acccaaataa gaagaatacc
     2221 gaagaactcc gcaagcgagc ggccgcagaa gacaaattaa aaatggctac tgacaaaatc
     2281 aaaccgccgt tatggctaga ttcgctagga aaggatacct ttgagtttat cgccgatgaa
     2341 ttgctgtctg tggatttaat cagtaatccg gacgtccata caatggctct atactccaat
     2401 tggtattcgc aatacgtttc tttagaaaaa cagcttcgaa aactacaacg agagtacaag
     2461 ttgaactatg cgcttgcgaa aaaggaggca gaggcgagag gtgagccgtt taatgaacct
     2521 aatgaattaa ttggtaatcc gctctctcgg cagatggata cagcgtctcg gaatctccgt
     2581 tcttttggcg ctgatttagg actatcacca gcagccagag ctaagttagc tattaagatg
     2641 gctgatgatg gtggtgatga cgatgacgac ttctaatatt ttggatatgt cttacacaga
     2701 acgtgtggac tattggcaaa gctatcttga ggagcaagct tcttggggtg gctttttaaa
     2761 atgtccatat ccggaattgt taactacttg gtatgcggaa cgattaatcg atggaagcat
     2821 accagccagc aaagaaaata ttcaagctgc taaacggcat atgcgtgatt tgcagcgcca
     2881 aggaacagat gattttcctt ggatctttga cgaagaaaaa ggtcaccggc ctattagata
     2941 tatcgaaaaa aaatgtaaac caactgaagg cgactttggt tcgtttgttt tgcaaccttg
     3001 gcagcatttc ataattggat ccatgtacgg atgggtacat cgtgatacag gagagcgtcg
     3061 cttccgcgag gctcttattt ttgttggacg taaaaacggt aagttttgcc gtttaaaaat
     3121 cgggcaaaat cggtgaaaac ctttattttt ggctttcttt tagtaataat tcgatagctt
     3181 tatcaagcaa tttagacata ggaaccatag tttcttccga catttttttt aatccttcgt
     3241 ataaatcttt atcaattgcg ttagaaattc taattctgtt ttttagtccg tatttgtcca
     3301 tgtgtgacac tcctttttat tttactataa catccgattc caccgcttgc aactaccatc
     3361 aattgatggt agtatataaa agaggtgagt agattgaaaa aatacattat ttacaaacac
     3421 ttaaataaaa taaatggcaa aatttatatc ggtgtcacaa atgatattgg cagaaggtgg
     3481 aggagcggtg ggattgaata taaaccacca aagaacgaaa cccaacacca cagaagtttt
     3541 tggaatgcaa tacaaaaata cgggtgggaa aattttgatc atttgattat tgaagaagac
     3601 ttaacgatga aagaagcttt tgagaaagaa aagttttata ttgaacttta tgactcaact
     3661 aataagaaaa aaggttataa catcgcaaaa ggcggaaatg gcggaataat atataaagtt
     3721 caccccaaag gaatgctagg gaaaaaacaa tctaaagaat tttcttcaaa tcacagtaaa
     3781 tgggcaaaga accataagaa taattgtatg acaaacggtg atgttgtatg gggtgttact
     3841 cacgaacacc caaaaggcat gttgggaaag caccacagca aagaatcaat tatgaaaaag
     3901 aaagcatata gtggtgaaaa tgctgtaacc tcaaaaccaa ttgtcgcaat tgaacgggat
     3961 ggaactaaaa gagaatttca cagcgctaag ctttgcatgg cttactattc gattagtaca
     4021 tgtgtttttt accgactttt aaaagatggc gccccttatg tgataaatcc taaagcgaac
     4081 tacagaaata aagaaaaaat attagctatc gaaggaatta tgttcaaaca taaagaagat
     4141 accgaggtaa gttagtagat tgcgaaaggt tactaactac cgtaacgcgt aggagttgaa
     4201 taaatataat gctcccaaga gtgtccgaca gcagataatt tgctgaaaat gtacgctaaa
     4261 ctgggccaga actgactgac cgatgaaaat gagggtgacc tccagagtgc gagataaaaa
     4321 gctcgcagat aataacaatc gaaaacatca ctaatttcag gcctttccac atacatggtc
     4381 gcttatgatg atgaacaagg cgccaacgtt tacgtattgg caaatgctcg tgatcaagca
     4441 agcttgttgt ttgataaggc cgcagaaatg gtcaaacaat cgccggcgct ctttaagaaa
     4501 tttggtaagc ctaaacgatc aagtattaat tatgctcccg ccttttctaa aatggaacca
     4561 cgcgcctcag atagccggaa attggatggg ctaaacactc actttggtat ttttgacgag
     4621 atccacgagt ttacgaatta caatctgatc aacgttatca agaaatcaag aggaaccaga
     4681 aaacagcctc tgatagttta tatcacaact gctggatatg tattagatgg tccgttgatg
     4741 tcttattttg agcaaggtgt ggactgtttg gaacatttgg aagatgacat cgatgaacgg
     4801 actttctatt atctggcaaa acttgacagt gcggaagagg ctgatgaccc aagattatgg
     4861 atcaaagcca atccgaatat ttgtctaatg aattttgttg gcatgctaga tgactatgtt
     4921 aaggataaaa aagatccaaa agaatatgct gactggatta ccaagcaatt taacttgttt
     4981 tccgatatcg atgagctgtc atttgtcgat atgcctacca ttaaacgaaa caataaaacc
     5041 atcgatattg aaacgctcaa aggtaagaag tgtgtcggtg gttttgactt gtccgaaacg
     5101 gaagacttta ccgcagccgt tttagaattt ccgcttgaaa caggcgaggt attcattttg
     5161 caacacacat ggatcccaca agctagattt gatcgagata acaatcaaga gcgtatcaaa
     5221 gcgtgggaga aggtgggaga tctaacgatt attcctggtg attacgtcaa ttatgaatac
     5281 gtcttaaatt ggtttgtaga aaattcgaaa atctatgaca ttgtaaaaat caattatgac
     5341 aaggccaagg cgctacgatt aaataaagaa ctagaaaatg caggatttga aaccaacgag
     5401 attcggcaag ggtttctatc attaggtggg ccaatgcaaa acttcaagga aatgctattg
     5461 gacggtaagg tgattttcaa caattccaag ctttaccgat ggtatctatc caacgtcaag
     5521 ctggtgatgg atcgcaactc aaactggatg ccgtctaagc agtccaagag tagaaaaata
     5581 gatggttttg cagcaagttt gaacagccac gccgaagtgt tgaatatgtt ggttaatcct
     5641 gtcggaaccg ggaaagtaac ctattactcg atttccgatt taatgaatat gtaagaaagg
     5701 tgtggaggaa tgagtatttt agatcgtttg cgttcttttg gccgagcgaa gccgaaagcg
     5761 agcaaacaag agtatttttt gaatgacccg ggattgatac cgtatttagt cggaaaagat
     5821 gaaatatcag aagggatttt ttccgtaatt agccgtgtat cgaacgtttt tgcgtctctc
     5881 cctctcaaaa tgatagatgt ggagtttggc caaccggacg actgtcctgc atacaacttg
     5941 ttgagcgaag gccctcgata ttttacaaag tttgattttt tccgggacgt ggaagttttg
     6001 agaaactacc aagggaatgc gtatgtgcag attttccgaa atatcaatgg agaagtagca
     6061 gatatggcgt tagtaaaacc tggtgcttgc catccagtga ttgatatgga tagcggggag
     6121 ctttactacc aagtaactgc gactgacaaa ggcagttaca agcaagttat ctatgtacat
     6181 tacatggaaa tgcttcactt taaacaaccg aggtttggcg gcttggaagg tacagacccc
     6241 acaaaagtat taacgaatac cctcggatat gatcgagaag tccgaaaaat ctctttaagt
     6301 cagcttaaag gaagtaatga agggctaaaa gttaagtttg ctagcaatat ggatgaagaa
     6361 gctaaaaaag ctacagttaa aaacattgct gatttttatc gacaaaacgg tggactactt
     6421 gtggaagaaa acggtgtaga aatcgaacgt ttacaacgag agctggtaga cagcaagctt
     6481 ttagatactg ataaagtatc tcgctccaga atcgctatgg tctacaatgt gccggagcat
     6541 ttcatcggga ataaccagtc gagttactcc tctcaggaac agctcaatat ggaatttttg
     6601 acgtacaatc tagtaccgac cgttaatcaa tatgaagcgg aactaaataa gaaaacacta
     6661 tcgagaaccg aaaaagcaaa aggttatcga tataagttta atgtcgcaag tttgctaaga
     6721 gctgacactc aggcaagggg acaattctat caaatcatgc gacgaggcgg agcatactca
     6781 gcgaatgatg tccgaagatt tgaggacttg caaccgataa ataaaaccgg tatggatgat
     6841 taccatattt ccggagacct atatccaatc gatatggatc caacattaag aaaaacaacc
     6901 tcgtctaaaa gcgtagccga aaacggttag gtttttttag tttgcaccga agggaggtgg
     6961 aaaaatgaaa aaagtgacgt taagcggcga tgtcgtggat aacgataccg cgtggcttta
     7021 tgactggttt gggatcaatt gtatctcacc agggaaaatt tctgccgctc ttacagaagc
     7081 agcgggggat gaagtagaac ttgatatctc atcgaacggt ggggatgtcc tagcggcaag
     7141 cgaaatatat accgctatcc gtgcctatcc gggaaaggta tccggaaatg ttgtgagtat
     7201 tgcagcaagc gctgcaagtg taatcgcttg cgcttgcgaa ccgcttagaa tctcacctac
     7261 ggcacacatc atgattcata acgcatgggt gaccactagt ggcaacgctg aggaattaaa
     7321 agccaatgca gaaatgttaa gcagtgtgga tgagtctatt gttaatgctt acgagatcaa
     7381 aacaggactc gatcggaaaa aacttgctga tttaatggcg aaagatactt ggttaaatgc
     7441 tcaaacagca gtagcagaag ggtttgcaga tgaaattatg tttgcagaag caccagtaac
     7501 ggtactcaat gcctctcaac cggttattcc aaaaaacgca gtaactaagt tgaaaaattt
     7561 aatactcaaa gcggaagcac cgcaagaaga aacactctta cagaaaaaac taaaagcctt
     7621 aaatggaggg aaaaacgaat gaatttagaa caattaaaaa atgcgtgggt cgaggcggga
     7681 agtaaagtct ctgacttaaa tgcacaactc aatgcagcat tggttgacga tgaaaaaaca
     7741 gaagaagatg tagtaagttt gcaagcacaa gtaaaagcag cacgggctaa acgggacgga
     7801 ttgaaagagc aagtggcaaa tatggaagcc gaacaagtct taaacgtcaa aaaagaacca
     7861 ttagataaaa aagatgaaaa cttgaaaaac aagtttatca aagactttaa agcgatggtc
     7921 aatggtgatc ctgctattat ggctactttg acatctaata cggatgaatc tggtaatgct
     7981 atcggattga ctattcctgt agatgtgcaa acgactattc atactttggt tcgtcggttt
     8041 gactctttgc aagaatacgt aaacgttgaa aaagtgacca ctaccagcgg ttctcgcgtt
     8101 tatgaaaaat ggtctgatat tacaccgttg actgctttgg atactgaaga cggtgaaatc
     8161 ccagcaaatg atgatcctgc actttacttg atcaaatact tgatcaaacg ctatgcaggt
     8221 atttccacag taactaacag cttgctaaaa gatactgccg aaaacatttt ggcatggttg
     8281 tctaaatgga tcgcgaaaaa agtagttgtt actcgcaata caaaaatctt ggcagctatt
     8341 gatggaatca aagcggcaca aaagaaagat gttacagatg ttgatggaat taaagatatc
     8401 gtaaacgtcc aacttgatcc agctatcgaa gctacatcta tgtttattac aaaccaagat
     8461 ggcttcaatg ttttagataa agtgaaacgt gctgatggat cttacttgtt acaaaaagac
     8521 gtagcttctg caactggata taccttctta ggtaaaccaa tcaagaaaat tgcttctcgt
     8581 ttcttgccaa acaaaggtac gcaagctact cctaaatatc cactgtacat tggtgatctg
     8641 aaagaagccg ttacattgta tgatcgcgaa aacatgagct tgctgacaac gaatattggt
     8701 ggtggagctt ttgaaacaga caccactaaa gtacgcgtca tcgatcgctt cgatgtgcaa
     8761 ctagttgatg atgaagcggt tgttttggct acttttacaa ctattgcgaa cgagacaccg
     8821 gcgaaagttt aaggagctga ttccttatga ttcttgatcc taaaatggat ttaggcgaaa
     8881 tcaaaaacgc actaaagatt gataccgatg atgacgatgt ggaagtaagc cgtgcggcac
     8941 aagctgcaat tgcatacatt agaggggcta tcgggaatga taagccctct ttttataagc
     9001 aagaaaatga aacggttgat ctgattaatt tagctatcct gcaattagca gatcattatt
     9061 acaacgctcg atcagcaacc gtgagtggga acttgcgaga gtacgattta ggttttacaa
     9121 gcctaatctt gcaactcaaa gcaagttatt tgctttttgt ggaggaggag tagcgtatgc
     9181 cccttatcca aacaggaaat ttaaatcaac gcatcaagtt tgtccgagat acgacggtta
     9241 aggatgagga cgggcaagtt gtcccgactt ctacaaccat tcttacttgc tgggcaagtg
     9301 tgcagacaca acgcctgaac gatattaaga cgtcgattgg tacggctttg gaaggaacac
     9361 tgacgttcat tatccgctac caacaaaaat cagagctgac caatgatatg aaagtgcgtt
     9421 ggaatggaaa aacgtttgaa atcattacga ttacgaaagg cgagtttgcg aaggacttca
     9481 cgacaatcat tgcaaaagag gtttcaaaat gagtgtagaa gtcgatgcaa ccgaagtgta
     9541 caaagcgctt agggaagtaa aagcaaacgt tcaacgagtg gaaagcccag cacttagaaa
     9601 agctggggag tacgctcaag aagagttacg acaaaacaca ccttactggg atggaacgaa
     9661 gtcaaacggt aaacgtggtt cgtatatgca agaacatgct aagaaccatg tggttacaag
     9721 ctcgataaaa aacggattgg tagaagtcgg ctatgacaaa gatgtttctt ggcggatgca
     9781 ctttatcgag ttcggaacaa tcaaacaacg tccaaaaggt ttcgtacaaa aaacacaaaa
     9841 gcaaatcgaa aaacaagtaa cacaaatcat tgctgacgaa gtaaaaagga ggctaggact
     9901 ttgaaaacgg cagtatcaca agtctattca attctgaata gcaatgaaaa aacaaagaac
     9961 attgattttt acaccaatag tgttccggaa tcagctcaaa cagtacctag ccttccagtt
    10021 ggcagaatta cagagatatc cggcaactat gaagattttg caagcaacaa tcctttgacc
    10081 attcaattta acgtacaggt agatgtatgg gtgtcaacca tgaaagaggt tgatgccttt
    10141 tattttgccc ttgatgaggt tatgaggggg aatggttggc aatgcgcata cacggaacaa
    10201 acagatgacg aggacttgga aggtgcaaag cggattatca aacgatatgt agcaaatatt
    10261 tcactaaatt aaaaggagag aaaatagatg gcaacagtag gattcgagag cgtcattttt
    10321 ggcgtaaaaa caggtgcagg cggcactcta aaagaattag tagcagataa gtcgaaaggc
    10381 ggagcgatcg aagctaaaat tactggatta ggcgcaactt ctaacacaac atacgcttca
    10441 aacgtaccgt tcttcattgc aagtaaaggg gtttcgtcgc caaaagttac gcttgacgtg
    10501 gcagacttaa tggataacgg catttacagc gaaatcattg gtgctaaaac cgtggatggt
    10561 gcaaatgtaa ttggttcaga aactgaagcg ccttacgtgt cggtagtcat ggttacagcg
    10621 aacaaagaag gaaaacgctt gttcatggga ttgacaaaag gaaaattcag tcatccagat
    10681 atcgacatga aaacagctga agacaaaggg gtagaattgc aaaccgattc catcgaaggg
    10741 gaattcattt ctgatgaacg tggctatgta tacttaacag ccgtagaatc agaaggaatg
    10801 accttacaaa aattcaagga cttggtaaat aacaaagagg gggagtagtt aaccctgcat
    10861 ctacaccaac gacagataca gggacaccaa aagaaccaga accaaaaatt gatacacaag
    10921 gttagccatt tttggctagc cttatttttt gtaaaaacaa ggaggaaaac aaatgattga
    10981 attgcaattg aaacttgacg gaaagaaaaa aacattcaaa caacaagata tttccgcacg
    11041 tgcaatgcgt gagtgtatca aattttacga gaaagcggaa aaagcagacc taactgattt
    11101 agaagcgatt gattcaatga ttgcaattac agcagatatt ttccaagatc cagcagttac
    11161 atttgatgct attttagacg gtttgactgc gagcgagtta gtaccggcat tagaaagtgt
    11221 ttttgaacaa ataaatgaac tgggaaacaa tgaaaaaaag cagacggcga gcaaaaagaa
    11281 ataagttttt ctgaagctag gaaagcaatg gatcaaatct acaaagattt aatcgaagca
    11341 ggttggacga tgagagatgt ggacgaagcc gactatcatt atttgttaca cctttttgga
    11401 gaagtggaga gtggcgaaga atatgtagat ggtgctgatt tcatcaaaca atttttatcg
    11461 gctgaagact tagtaaaact tgaggaagga ggtaaataat ggcaggaaaa ggacaaccgg
    11521 caggaaatat caagctaggg attagtttag atagcactag ttttggtaac acgctggacg
    11581 aaatcaatgc gaaagtcaaa caagctgagt cgaatatgcg tgccaatcta aaggcttatg
    11641 attcagcagg acgttcatac gaagcactta gtcaaaagac gaaagacttg tctacggtta
    11701 tggaagggca aaacgccaaa gtaagagaat taacaaagcg ccgtgatgaa gcgattagca
    11761 agtatggcga ggaatcgaaa caagttgcta accttaacac acagataaac aatgctaccg
    11821 caaaatataa tgcttacagt cgccagttga acgacacaaa aaaagaattg gtgtattcca
    11881 aaacagccgt caatgattta tctaatgaaa tcaaagaaaa tgaacgacaa atgaacgccg
    11941 aagtaaaagc gttgaaagcc gctggtgatg aatctggtgc ttttgaagca aaacaaaaag
    12001 ggctagccaa acaaacggaa ttatccgaga aagctatcga agaacagcgc aaagttgtga
    12061 aactgatggc tgatgagttt ggcgattcag caaatgaaac cgaagatgca aaaagggcat
    12121 tagaaaagtt agaacgacaa agccaaatat ctagcaggca attagaagca ctcaaaagct
    12181 ccagcgatca atcaggaaaa gaaatagaag attttggcga caagtccaca aggtcagcta
    12241 ggaaactgga cggactaaaa gacaaattaa gctcgctaaa aagcgcattt tcgtttggtg
    12301 cagttgctgg attagcgcat aacgctatta gcagtgtagt aagtggcgtg caaggtttgg
    12361 ttggcgaagc agtaaacgca tcggattcat tgatgaagtt ttccaaaacc atggagtttg
    12421 ctaactttgg gaagtcacag atagaaagct cgaaaaaaga aatgaaagac tacgccgata
    12481 agacggttta tggtttagaa gaaattctga acacaaccgc acaattggca tctaatggga
    12541 ttcctaacta tacagaacta accaaggcgg caggtaactt gaatgccgtt gcaggcggtt
    12601 ctagtgatac attcaaatcc gttgccatga tgctaacgca gacggcagga gctgggaaac
    12661 taacaactga aaactggaat caattagcag atgcgatacc gggtgcttca ggattgttac
    12721 aagacgctat gttgaaaaac ggagcttata caggaaactt ccgtgatgca atggcgcaag
    12781 gtcaaatcac ttccgacgag ttcaaccaag caattgtaca gttaggtatg aatgacggag
    12841 cagttaaggc agccacttct acagacacat tgagcggttc ttgggagcag atgaaatcca
    12901 ctgtaataaa tgggctacaa agtattatag aaaaaatagg cgttgaaaat atcactggtt
    12961 tcatcaacag agtaacaaaa gggattgaaa attctattcc taaaattact caatttatag
    13021 gttggttgag agatattgga acgtggatcg ttgaaaatag agagccacta acatggattg
    13081 tcggaatcat aggcggaatt acattagcag taaaagcatt gaacgtagca agtatgttgc
    13141 tggcaattac tggcggaaca ttggcagccc cttttgtggc gattggtgta gcattaggcg
    13201 cactagcagg tgctttggtg atagcttata caaaatctga aacatttaga aatatagtca
    13261 atgcggcttt tacagctgtg aaaaacgtag ttatgagcgt tatcaataat ttggtggaat
    13321 actacaaaat gttgtggagc gtgttgcagt ggctttggga aaaaataaaa gaatgggctt
    13381 catggattgg caataaattc attgaaatga agaacagcgt tgtgaatacg gttcagaacc
    13441 tatggaatgg cgtgaaaaac ttcttcagca atggcgttgg agatacgtgg aataaggtag
    13501 tcggttgggt aaaaaacatt ttcaacaaag caactgaatt gaagaacaac gtttctgatg
    13561 taatcggtaa cctgtggaat ggtatcaaag acacattccg tagaggtatc gatacggtat
    13621 tcaattggtt ttcagaacta ccaacgaaga tgaagaatgc cattattggc ggtaaaaacg
    13681 ccattgttga tgcgttcaaa agtattttca acgcagcact taaagcgata ggtaaaccag
    13741 ttaacgcaat catccatgga gcttcatggg tactagaaaa attgggcgca gaacctctac
    13801 aagaatggga tgtaccacaa tacgctacag gtacaccagc aggtgggcac ccaatcaatg
    13861 gtccaatgat ggttaatgat ggacgtggag cagaaacagt tatcacacca gatggtagag
    13921 ccttcattcc taaaggacgc aatgttgttc taaacgcacc aaaaggaacg catgtcttga
    13981 cagcagaaga gaccgcgcaa cttcaaggtt ctaaagctcc taagtatcgt tacaaaaaag
    14041 gtactaactt ctttggtaac atgtgggata gtgtgaaaaa ggttgctggt aatgtaggtc
    14101 acacaattaa aaacgtagta ggtgacgtgt gggactttat ttcagacccg ggagcgttag
    14161 ctagaaaagt acttgggggg ttagatgtat taggtggatt gacaaagtat ccattagaag
    14221 taggtaaagg catcctatct aaagcaacaa gtgcactgac tgaaaaaatc actgggttgt
    14281 tctcatctgg taacttagat acctccgtag gaacaaatgg cgtctataaa tatttggctg
    14341 atgttgctaa gtctgtgatg aagaaattcc caggatttgt ggcaactagt gggtatagac
    14401 caggtgaccc ttattcacat ggtaaacgta atgccattga tattgcatta ccaggcgtca
    14461 caggaggctc acctagatac acagaagcag caaactacgc ttttgacaaa ttcgcttcca
    14521 aaattggtta cgtaatcact aatgggaaag ttcgtgaccg ttcaggacaa tcaggtcaac
    14581 cagcaactgg tgcatgggag ccatggcccg atggagatca ctatgatcat gtgcatttaa
    14641 acggtgtgaa agacccacaa aacactcaaa tttcaggaga tagcgtggga ggcagtgggg
    14701 tagaaagatg gcgcaatgta gcaattagag cgttgaaaat gaccggtcaa tacagtactg
    14761 caaacttaaa tgcattacta aatcaaatgc gtacagagtc aaatggtaat cctaatgcag
    14821 ttaacaattg ggatattaac gccaaaaatg gaacaccatc aaaagggttg ctccaagtga
    14881 ttgacccaac attcagacag tatgcaatgc caggattcaa cagcaatatt tttgacccac
    14941 tatctaacat cttagcttca atcagatacg cactatcaag atatggctca ctaacaaatg
    15001 cctatcgtgg agttggttac gcaaacggtg gaattgtaaa ccaacatcaa attgcggaaa
    15061 tcgcagaagg aaacaagcca gaaattatta ttccgttaga taaggctaaa cgatcaagag
    15121 cgatgcaatt attgtctatc gcaatggaca aactaggagt ttctcctaaa acttatggaa
    15181 atgcgacaac tgtttccagt gattacgaaa tcctagaagc cattgaaaaa caggcggcaa
    15241 caacgaatca gctgttatcg ttattgctgg catttttcaa aggaaatagc cgaactgata
    15301 gagatttagc tttagatatt cagaagatct tggttaggag gatgtagatg cgaactgtat
    15361 tactgagaaa tagaataaac gaagaaattg atttgtccac agaagactat tttgctactg
    15421 ggttgagtaa tatggggttt gaagtaaaaa aagaacatgt gggacagtgg gggaatttca
    15481 gagaaagcag tgagagcgtt gaaatatctg aatttcagtc atctgtaatc atttctgtgc
    15541 atgggtttcg agaaaaagaa ctgtacaatt cacttgtgca gttcctatcg gaaggtccgt
    15601 ttgaactgga atttgctttt gacggcgaaa caatggtaag aagatgcagt ctaaaatctt
    15661 taagtaagac tgaaatcgat ccgaaaacat cgttactaac agacacttta gaactatatt
    15721 tcacatcaaa ctggtattcc gtaaaacgcg aaaaattaat tcagcgaccg aatgtagtga
    15781 aaacacgtgg taaggttttt ccgtataaaa gatcttatat ctacactcaa aacttgtggg
    15841 agaaaaaagg tgtattcaaa ttcaataata attctgtata cctaacgaat agcaaggaac
    15901 ggatgtcccc gctaaaaatt cgtgtgattg ggaagtgttc aaatccgtat tgggaagtaa
    15961 tccaaaactc acaaatcatc gcaacggatg gatacttcat agatatgact gaaacacaga
    16021 ctctagaagt atcaagcctt tttgaagata caacggcgat tttaaaagat attgcagggg
    16081 tagaatcttc tgtctaccaa caacaggatt atacaaaaac taattttgtt caggccccaa
    16141 ctggagaatt cagcattgtg tttcatgttg ggggggcaga cgtagagatt gagttatacg
    16201 aggagcgtga tctgttttga ttttagcagt cacgcttttt catcgtgatt taatgcttta
    16261 taacgaacat ttattctcta acagttttga atttggtgtg gacgagatca acgaagaggc
    16321 tagtagcttt acgatggata agtacgttcc tgtaaaaaca ggcgattttc tactagcaaa
    16381 atatattcct agtggaaaat ttgcatattt tggtgtaatt acgtcgcagg aagacgaaaa
    16441 aatcagttgt aaaagcttac ttagtttagc tgatagtgag ataccgactg cacgtgtgtc
    16501 aggagataac tacgaagagc atatccggcg gttgattgaa tactatttgc tgaatgatcc
    16561 aacgaaacaa ctaaaagata ttttagacgt caaagcagaa agtgcgacct ctcattcgta
    16621 tcaagctacc gatacgaata aacacaagtt aagtgcgtat attctcaatg gtttcaaaaa
    16681 atacaacgta aaatggtatt tcaagggaat tcaaaacaga aaaatttata caggcatacg
    16741 tgctgtaaat gaatcaattt acattaaaga caattcttct gaatttagcg attgggatgt
    16801 gtttgttcag gcgccgggcg ctggaaacga gaacaagcta ttaatcgttg ataaagcaat
    16861 gaaagatata gagaaaccga taatactgtc aacatggtat ttggacgaag aaaacaattt
    16921 gacacaagat ggatcgaaag aaaatatcac gaaaccaact gtcaatttgg ttaatatcta
    16981 cgatcaaacc gcagaagata aagcatccta cgaagatgtg gcaaaatcag agttgaaagg
    17041 caatacgtat tcgcatgaaa tcaaagtgaa cgttgtaaga aatgcaaaaa atttgaatgt
    17101 cgaaacgatt gaaacaggga tgtttgccac gatttcttat aaaggaaaga tatacaagtc
    17161 ggttttgaca gcttggcgaa tatcaagtga taaggagttt gtggaattga ctttcggaaa
    17221 cataagaagt cgttttatgg attattttga agataatggg ggataaaaaa tggttagcaa
    17281 tgtggatgga tatcaatttg aaaacgtgaa agtaagcgca gaaaatgatg ctagacttta
    17341 tcacgtttta tataatcgga aaaatcaggt tattgatggt tacgatcagt ctatgaattt
    17401 atcttcaagc ggattaacag taaaagttgc tgcaggagca gcgattattc aaggtcgtat
    17461 ggtcgttgtt cgacaagaag aaagtataac agttccagca aactcaagtg gttatatagc
    17521 attaacagtg gatttgacac aagaagttat acctggatct attcttccag aatcggaaga
    17581 atatgaatgg actaataatc aagtcaagct agaatttata acaaaagtta taaaaggtaa
    17641 tttaaacaat ggtgataagg tctataattt accactatgc tcagttaatt ctactggatc
    17701 aactgtttca atctcaaaga tatcggatag ttacgagctg actctctcta aaggagaaat
    17761 tttgtggagg gggactgcgt taatgcatga tactcaaact gtccaacctt caaaaaaaat
    17821 ttggcagaca gtttcaggtt ttttgttagt atggctccca tacgaaaacg ggcaagcaat
    17881 tgaggataga tatgtaacta cgccttttta taaggagcgt gtaactttta ctaatgtttt
    17941 aggagaaatt gtttcaggat ttgatgacta tcacaaaaag tggtttagta aacgaataaa
    18001 ctataattcg aatactaata tatttacagg tgcagcaagc aatgcaagtg gagataacgc
    18061 aaatatggtg cttaggtata tagtttcttt ttagttagga ggtatggaag agtggcaaat
    18121 ttagaaatta aattatctgc aaataagaga cagccttatc tacgtcaccg tgttgttggt
    18181 agagttggtg atggggggct cacaacaatc aatgtacaac ttcttgagga agatgaaatt
    18241 acaccttttg taattaatct aaacggtact ttgaaatttg tgggcgaagt ttcaaacggt
    18301 aactataccg agggagaacc agaaataatc gattcgacta atgggttaat tagttacacg
    18361 ttcactaagt caaatttcag cacgagtcat caattcaaac aagcatattt tgaatatgta
    18421 gatcctaacg gcaaaaaagt aacttttcag aacttcatca tagacgtgtt agaacgagtg
    18481 gatattaatt cggagcaagc gaaatactat atttcttcat tggaaaaatt acagagtgaa
    18541 atgcaaacca ctttcaatca gttcattagt gataaacagg tccaatacga tcaaatctac
    18601 tcgaaatata acgaattagt aagattgata aatgaatcag ataagcaagt aaatgatcgt
    18661 attgatcaaa ccaatcagca aatcggcgat ctcggcaagc tgaaaaagat gtacagtaac
    18721 agcatcgact ttgggggcta tgattattcg gggaatccga atttgttaag taagctatca
    18781 tacgacttaa ttgaaaatca aaatacttca gctggaacac tttctaaggg tgaaaactcg
    18841 tttaaatata ataagatatc agctgaagtg gaaggtggag tagagttata ttataaacga
    18901 agaggtatag ctaactggtt accctctaat aaaacgcttg taatgactgt taagcttaga
    18961 gctggagcgg actatagtcc agttgacgga aaacttatac tgattagata taggtatgtt
    19021 gacagtggaa ctggcaagat tgttttagac ttacctatta acagtaattc gataactcag
    19081 gaatggaaag agtttagtat tactggaact actccaacat ttagcccaca agcataccat
    19141 ccttggatac aatttagggc tcaagatggg gtacttgggg aaatagaaat gagctatgac
    19201 atcaaaatcg aagaaggctc tacagctaca ccatatcagc ctaacttact tgatgactct
    19261 tactggctag gtaaaacacc gttgggtgag aatttgataa cgaatgccaa gttcccaatt
    19321 ataacacgaa gcaatcctat ttctggtttt gatatttcag aagaattaat aataggtgaa
    19381 acttatacgg tatctttaaa agggactaag ccagctacta aagaattcca cttatactac
    19441 ggagaagcaa attaccagca gttgcagtat caggcaactt tacttcctgt ggaaggatta
    19501 acagatgtgt ggagtgccac ttttacagcc agaaatacta gtgaaacgac aaatttactt
    19561 agagtagcgt tatggcaaaa acctaattct gcaacatatg atactgttca aattgactgg
    19621 ctcaagattg aaaaaggcga cacacgcaca ccaaatatca gtcagtttaa atactttggt
    19681 gaaggcttga aagacagtaa caatccgaac gactacagct gggatgtcac accagagtat
    19741 actgaaaaaa gcttgaataa tacggttagt ctgactgaac cgcaatccat cgaaggattg
    19801 aaaaatttcg aagatggtat tcaatcaaaa ggaaaatctg tattgacatc agacgacaac
    19861 aaatatgaag tcgtaacctt aacagttaca aacgggaata ccggatcagc aaagctttat
    19921 cgtgaaggaa aaaccgtcac tatttatttt tttgcgttga atgggaaaag cagtggtgga
    19981 aatgattcaa cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa
    20041 ctggttggct cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat
    20101 ggagccatta aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact
    20161 tacacgattt agaaaggcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta
    20221 cggattcgag caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt
    20281 cgaaccaccg attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa
    20341 aacatttgga aatatggccg tactggcgga gagtatgcag gaaaagtatt ggacgacatg
    20401 cttgtatccg ttccttacac agatcagcca ccgctcgaag gggttcgttc agatggcgaa
    20461 ccgctaacga ttgctgatca gatgtttgat cctaaattga accaatggat tattttagcg
    20521 aacgcgttag atcacaacga tttaaacaat ctcaaagcga tgtacgaggc tcttgaacat
    20581 gaaaacggca acctaaaaca gctaaatgcc aaactcatgc taagcgatgt agcgattaaa
    20641 caggaaaata ctgcattgaa agaaaaagct gacagtttag cacaaatcaa ttcaaaaatg
    20701 atgcttgctt cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca
    20761 gcttcaaagg gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg
    20821 gggctgtttt actgacgatc aagttcgtct attcgttcca ctatgtatca cagatgaaga
    20881 agcagataga attattagca aagaagagag cgcatcttaa gtgatgcgtt tttttgttgg
    20941 aaagttggtg gaacatgaaa gaagaagcgc tccaagacgt tgtggagaga ttagtaagaa
    21001 ttgaaacaaa attagacaac tacgaatcac ttagagaaaa agctgatagt gcaaaagatt
    21061 tggcagacaa agcctactca gtagcgctaa acaacgcaga agatatcaaa gaaatgaaag
    21121 ccaataataa atggtcgtgg ggttacatga tcggtttagg cattacgatc attggctatt
    21181 tcttgactaa attgtaaagg agttaagaag aaatgatttt acccgataag tattatcaag
    21241 tcattaaatg gacggtttta acagtattac cagctgcatc tgttttagta gccacgttag
    21301 gaaaagcgta tggatggaat ggaacagata tgacagtact cactatcaat gcagtagcaa
    21361 catttttagg cgttatcact ggtgtgtctg catataactt aaaaaaatag gaggaaaaaa
    21421 atgaagaaga aaatcatttt atcattgagc ctaataatgg ctcttttttt attgccaatt
    21481 aacgggttcg cctatacgat caacaatgaa tttaatttag gtgtaaatga aggtagctca
    21541 caagtagcaa ataaccaata catcctattg catgaaacag caaacgaaac tgcgacagga
    21601 cgaaatgaag cgcagtacat gaaacgttct tggtacaatg cctatacagc gtatattgtt
    21661 ggcgatggtg ggatcgttta ccaagttgga caacctggtt atgtacaata tggcgctggt
    21721 tcatatgcta atgcaaatag tcctgtgcaa atcgaactgc aacatacgca cgataaagta
    21781 acgtttgaaa agaactacaa ggcatatgtt gaattagcga gagattccgc taataaatac
    21841 ggtattccac ttacattaga cacaccgtat aatcaaccag gaatcaaatc acatttatgg
    21901 gtaactcaaa acatttgggg cgatcacact gatccgtatg gatacttatc agaaatgggt
    21961 gtaagtaagg aaaaactggc ttacgattta gctcatggat ttacagacga aaacccaacg
    22021 acttctgaga acaagcctgt cattgatcca acccgagcag gtgcagcaaa tccaacttta
    22081 tcagatggaa cgaaccattc tcacattgat cagtttggag aaatcgaaaa tgcgaactta
    22141 cacgtcgctg gatggcacat cgctaactat aaatacgagt atattttcat tatggactac
    22201 aatactggga aagaattagc tcgagtaaga gctgatggaa tttatagacc ggatgtaaat
    22261 caagcttata atacttctgg aaacgttggc tatcatgtat cttttaacat gcgcaatttc
    22321 cctaataaga aagtatacgt catgatgcgg gcaacgaatg atccagaagg aaacactaaa
    22381 ggcggtgcgc aagatttcca tgacaaacgt tggtatttaa atattcctaa acgataaaaa
    22441 tagctcctcg ttgaggagca gtacatagaa ttgaaaatta acgttaaatc aaaaaaatat
    22501 ttacaagatg tttatttaag tagataataa aagaaaaaag caggtgaaat tgtgacagca
    22561 gaaattggta ttatgaataa aagtggaatc gtgttagctt cagattcagc atctacgatt
    22621 ggagatagca aagtatataa tactgcaaaa aaattattta ctttggattc catgcattct
    22681 gtaggtatca tgatatatgg taatgctgaa tttaatggta ttccttggga aataatcatt
    22741 actcaatata agaaaagcat tggtagttct gtttttaata ctttggaaga atacgcggat
    22801 aattttatag aatttgtcaa aacagcatct tttattagaa gtgaacagac cgagcaagaa
    22861 caaatgattg gggtttttca aaaaataatt tcaggtttat ttgagagtat agaagtagat
    22921 attaactttt taataagcca aggtacacaa atagacaaag atgtcctagt gaagttatta
    22981 caaacaaaaa tgaataccaa tttgtctcaa cagtcccaga cattcatttt agatattgaa
    23041 aaggcgttat ttcttagtaa ctatggagaa atattaaaag atattcttaa tagtatttct
    23101 acaatggaag gtgtttcaga agctatatca gaggaaattc aaagttacgt ttatgaaatt
    23161 ataattagag acgatgttta ttcttcccct acaggaatag ttatagcagg atatggaaga
    23221 atggatattt tccctaaact atattcttat aatatgtttg gatttgttat gaatattctg
    23281 aaatactcag aatatgaaag tgctcaaata ggaaatgaca atggttcatt aagatctact
    23341 atattaccat ttgctcagtc tgatgtggta aatactgtag ttcaaggggt agatccacaa
    23401 attactaatt atctgtcgag tcaagtcgat agttttgacg ataacggaaa aaatacctat
    23461 attaatatta taaagaatat ttcagagttt caacaaaatc aattcatatt tccgttacta
    23521 aacatgattg ctttactccc agttgaagaa acagcaatta tcgcagagac tttattaaat
    23581 ttaaccagtt ttaaacgaaa atatactact tctgtagaaa cagtaggtgg cccaattgat
    23641 gttttagcta ttacacctaa tgatggccct atttggatta aaagaaaaca ttattttgat
    23701 attgataata atattggata cagattgcga aaggagagag cgaatgatta caataattaa
    23761 acctcaggaa aaatcccctg ttttgaaatt taatgagagc aatctagccc ataataaaat
    23821 gagtataact gatacagtat ttgatgtaat ggttgagcaa caaaaaaaaa ttaataataa
    23881 caatagacaa agaaaagaaa ttaaccaata ttaatgtttt ttctgttgcg ttttttgcag
    23941 tcataattta aaacctcgct caatcttggg cgaggtcttt ttttat
//
LOCUS       DAZKOB010000035        23798 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000035 DAZKOB010000000
VERSION     DAZKOB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.035"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG8M6_001342"
     CDS             complement(164..388)
                     /locus_tag="ACG8M6_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0457019.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG8M6_001343"
     CDS             complement(586..2166)
                     /locus_tag="ACG8M6_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0457020.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG8M6_001344"
     CDS             complement(2567..3943)
                     /locus_tag="ACG8M6_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0457021.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG8M6_001345"
     CDS             complement(4071..5159)
                     /locus_tag="ACG8M6_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0457022.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG8M6_001346"
     CDS             5331..5765
                     /locus_tag="ACG8M6_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0457023.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG8M6_001347"
     CDS             complement(5843..6376)
                     /locus_tag="ACG8M6_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0457024.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8M6_001348"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG8M6_001348"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0457025.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8M6_001349"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG8M6_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0457026.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8M6_001350"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG8M6_001350"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0457027.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG8M6_001351"
     CDS             complement(10101..10679)
                     /locus_tag="ACG8M6_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0457028.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG8M6_001352"
     CDS             11001..11471
                     /locus_tag="ACG8M6_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0457029.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG8M6_001353"
     CDS             complement(11579..12805)
                     /locus_tag="ACG8M6_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0457030.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG8M6_001354"
     CDS             complement(13559..13759)
                     /locus_tag="ACG8M6_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0457031.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG8M6_001355"
     CDS             complement(13893..14501)
                     /locus_tag="ACG8M6_001355"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0457032.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG8M6_001356"
     CDS             complement(14586..15056)
                     /locus_tag="ACG8M6_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0457033.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG8M6_001357"
     CDS             complement(15104..16018)
                     /locus_tag="ACG8M6_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0457034.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG8M6_001358"
     CDS             16164..16967
                     /locus_tag="ACG8M6_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0457035.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG8M6_001359"
     CDS             17145..18092
                     /locus_tag="ACG8M6_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0457036.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACG8M6_001360"
     CDS             complement(18160..18453)
                     /locus_tag="ACG8M6_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0457037.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACG8M6_001361"
     CDS             complement(18481..19146)
                     /locus_tag="ACG8M6_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0457038.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACG8M6_001362"
     CDS             complement(19286..19918)
                     /locus_tag="ACG8M6_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0457039.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8M6_001363"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG8M6_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0457040.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8M6_001364"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG8M6_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0457041.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8M6_001365"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG8M6_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0457042.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8M6_001366"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG8M6_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0457043.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         7062 a         4833 c         3828 g         8075 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactat
//
LOCUS       DAZKOB010000036        23642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000036 DAZKOB010000000
VERSION     DAZKOB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.036"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8M6_001367"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="ACG8M6_001367"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0457044.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="ACG8M6_001368"
     CDS             complement(1171..1866)
                     /locus_tag="ACG8M6_001368"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0457045.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="ACG8M6_001369"
     CDS             complement(1879..2595)
                     /locus_tag="ACG8M6_001369"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0457046.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3533
                     /locus_tag="ACG8M6_001370"
     CDS             2925..3533
                     /locus_tag="ACG8M6_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0457047.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="ACG8M6_001371"
     CDS             3858..4181
                     /locus_tag="ACG8M6_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0457048.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="ACG8M6_001372"
     CDS             4363..5733
                     /locus_tag="ACG8M6_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0457049.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="ACG8M6_001373"
     CDS             complement(5863..7164)
                     /locus_tag="ACG8M6_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0457050.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7518..9509)
                     /locus_tag="ACG8M6_001374"
     CDS             complement(7518..9509)
                     /locus_tag="ACG8M6_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0457051.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9653..9919)
                     /locus_tag="ACG8M6_001375"
     CDS             complement(9653..9919)
                     /locus_tag="ACG8M6_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0457052.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8M6_001376"
     CDS             complement(10429..12555)
                     /gene="pnp"
                     /locus_tag="ACG8M6_001376"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0457053.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8M6_001377"
     CDS             complement(12743..13012)
                     /gene="rpsO"
                     /locus_tag="ACG8M6_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0457054.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13263..13826
                     /gene="def"
                     /locus_tag="ACG8M6_001378"
     CDS             13263..13826
                     /gene="def"
                     /locus_tag="ACG8M6_001378"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0457055.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14079..14600)
                     /locus_tag="ACG8M6_001379"
     CDS             complement(14079..14600)
                     /locus_tag="ACG8M6_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0457056.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14603..15445)
                     /locus_tag="ACG8M6_001380"
     CDS             complement(14603..15445)
                     /locus_tag="ACG8M6_001380"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0457057.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15438..16163)
                     /locus_tag="ACG8M6_001381"
     CDS             complement(15438..16163)
                     /locus_tag="ACG8M6_001381"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0457058.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16339..16635
                     /locus_tag="ACG8M6_001382"
     CDS             16339..16635
                     /locus_tag="ACG8M6_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457059.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16891..17065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17203..18795
                     /locus_tag="ACG8M6_001383"
     CDS             17203..18795
                     /locus_tag="ACG8M6_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0457060.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18808..19548
                     /locus_tag="ACG8M6_001384"
     CDS             18808..19548
                     /locus_tag="ACG8M6_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457061.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19716..21065
                     /locus_tag="ACG8M6_001385"
     CDS             19716..21065
                     /locus_tag="ACG8M6_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0457062.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21193..21783)
                     /locus_tag="ACG8M6_001386"
     CDS             complement(21193..21783)
                     /locus_tag="ACG8M6_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0457063.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8M6_001387"
     CDS             21930..23282
                     /gene="brnQ"
                     /locus_tag="ACG8M6_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0457064.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6770 a         4761 c         4502 g         7609 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcaaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattctg tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 cttacccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat aatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttatcacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aaaacggtat tcaaaagctt gcatctacgg cattaagccc
     7381 aaacagatga aaaacatgag aagctgtttt tatctgctcg ttcttactgc ttgttgcagt
     7441 acagcttttt catgacctct gataaacggt gttcaagtag ctgacattcg acattagttc
     7501 aggtcaaagc gctactttca caaccgctct ttcaaccatg ttttccgagt atttccaatg
     7561 ataagcggaa cagtttcatg ttttacttcg ctgtactcta gcccaaacca tgtttctggc
     7621 gaggtagcga tttttttgat tgccagtttt gcctgcatga tttgacgttg ccattttcct
     7681 agagttgtca cattggaaac tacttcatca atcaggacaa attggaaatc gccaacttct
     7741 cgccctggtg tcagagagta tctttgtggc tgttttggca aacggccttg tttcatcaaa
     7801 tcatggatga cttggcgaac atatacgttg atttcctgtt ggaccctgaa acctaagaac
     7861 agattcactt ggacaataaa atcagtgtcc atcatatcta cttgatattc tttcgtatat
     7921 ggctcatccg tcacttccac atgaacaaac caatagactc gtgctctttt aggccgttta
     7981 tctaaaatcg aatagatgaa ctgtcggcca actttatctt cagccatatc aggaaccaag
     8041 aagaccacat ttgtctgata gattggtaaa gaagtatcat cacgtaattc tctaagctgc
     8101 gggatataat ctctcaaaga cacatcctcg gttgacgatt cgcgaataat gttcccttgt
     8161 tcccaaataa acatcacgac taagatgatc aaaccaatca gaacggctac aaaaccgcct
     8221 cggaagaatt tgacaacact tgagataaag aaaacgactt ctatcgcacc gaagaaaaag
     8281 gtaactaaat atgcaagcca acgagaatgc ccattttgga taagataaaa gtaaagcagt
     8341 gctgttgtca tcaacatcgt cacagtgatc gatagaccgt aagcagcttc catatgatga
     8401 gacgttcgga atgtgacaac cactagagaa caggcgatcc ataaaatcag attgactgct
     8461 gggatataca tttgcccgat actgttccca ggatacatga tctttaaccg aggcagcagt
     8521 ttcaacttga ttgcttctga aactagcgta aacgaacctg taatcaatgc ttgcgaagca
     8581 atgactgcag cgactgttgc aaaagcaacg ccgatcacga tccaactttg aggcatcatt
     8641 tggaagaatg gattcaggtt cttgatttgc tgtgcttcag ggttttgata aacattcaac
     8701 aaccacgcag cctgcccaaa ataattgata accaaacaaa ttttgacgta aggccagctc
     8761 agacgaatat ttcgtttgcc gacatgtcca agatcggaat aaagcgcctc tgcacctgtc
     8821 gtagccagga aaatatttcc aagaatgaac aagcctaatt tgttatctgc actaaaaagc
     8881 agctggatag cataataagg attcagtgca cggataactg tccagtcttg actgaagttc
     8941 attaatccca ttacgcctaa aaaagtaaac caagcgaaca tgattggtcc gaatgctttt
     9001 ccgacaatat ctgtaccaaa gcgttggacc atgaataaaa caaaaataat aactaaagtg
     9061 ataatgacga tcacgttctg gttattaccg aaccggtcaa aaaactgcgg aattcctcgc
     9121 aagccttcaa tcgcagtcgt taccgtcaca gcaggggtca ataccccatc tgctagtaag
     9181 gcagctcctc cgatcattgc aggaatgatc aaatatttcc caccttttcg gacaagtgtg
     9241 tagagtgaga aaattccacc ttcaccatgg ttatctgcat tcaatgcaat cacgacatat
     9301 ttgattgttg tcagtagagt caatgtccaa aaaatcaatg agactgatcc gataacaaaa
     9361 tcaggattga ttcctgacaa tccgccatta tcttcaacaa tcgatttcat aacgtaaagc
     9421 ggacttgtcc cgatgtctcc gtagacgact cccatcgcta caagcaggcc agtcattgat
     9481 atctttttgg tattgtgcgt tgttgccacg tttttccctc atttcttgtt atatggatat
     9541 cctttaggta agcttgaatg atagatgata ttcttatcca tgacacccgc tttcctaaat
     9601 tgcttctaat tatccaagta acacactgaa gtgtcaactt atttgaataa aattattctt
     9661 tctttggacg ttctctgata cgtcttgcca caaatgaata aagagcaagg caaaaaattg
     9721 cacccaataa tgctggtatc aaataaaaac cgccgagttc cggtcccagt ggtcctagta
     9781 aaaattcgcc gagccaacta cctaaaaaac cgataatgat atttccagat acgcctcccg
     9841 ggacttcctt tcctaaaatt gcgccagcta tcaaacctaa tataccgccg ataaataacg
     9901 accataaaaa attcagcatc tcctttccct cctttatcca tatttctcac ttttatttta
     9961 cgctttattc agaaggactc caaaaatatg cgccaagaaa ctccagaaaa aaagaagttg
    10021 taacaaaagt gttcagctta tttccgtaga agctgcttct ggaacaccgt gaatcagagg
    10081 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    10141 attttgccgt tttttgagct aatatcgagg gacagctgat tgaacatcgt ttattggaga
    10201 ggttgtaaaa aagctgttct gcatcaagta ataagaacag ccaaataaaa acagctccta
    10261 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattc
    10321 gaaaaaaaag gactgtgaca cggcttttgt cacagtcccc ttttttcgaa taattattgt
    10381 ccccctgtca ccttcacgat cacaagtaca ttattcctat gaattaaatt atttttcttc
    10441 atttttcgtt gaaccatctt cgttcaacat tgcttttctt gacagattta cgcgtccttg
    10501 tttatcgatt tcagttactt tcacaaggat ctcatcacct agtttaacaa catcttcgac
    10561 tttattgaca cgatcgtttg ctaattggga aatatggaca agtccatctt ttcctttgat
    10621 caagttgacg aatgcaccga atttttcgat acggacaact ttccctagat aaacttcacc
    10681 aactttgact tctttggtca aatcttcgat gatcttgata gcttttttga tcatttctgc
    10741 atcagctgaa gcaatactta cttttccatc ttgatcgata tcgattttca caccagtttc
    10801 atcgatgatt ccgttgatcg tatcgccgcc tttaccgata acatctttga ttttagcagg
    10861 ttcgatctgg atcatttcga tttttggtgc atattggctt aattcttcac gtggagcagc
    10921 aatcgttgaa gtcaattctt ctaagatttc catacgtgct tgttttgctt gagttaatgc
    10981 ttcagttagg atttgttctg tgattccttg aatcttgata tccatctgta aagcagtgat
    11041 accatctttt gtaccggcaa ctttaaagtc catatcgcca agatgatctt ccagcccttg
    11101 gatatcagtt aggatcgtat agttttcacc atcagaaaca agtcccatcg caataccggc
    11161 gactggcgct ttgattggta cacctgcatc catcaatgct aatgtgccag cacagatact
    11221 tgcttgggaa gaagaaccat ttgattccaa tacttctgct actaaacgga tcgtataagg
    11281 aaattcttct tctgaaggga ttacttgtgc taacgcacgt tcacctaatg cgccatgtcc
    11341 gatttcccga cgccctggag agcctgcacg tcctgtagaa ccaacagaaa attgagggaa
    11401 attgtagtgg tggatgaatc gtttgctgac ttccacccct aaaccatcaa tgatttggtg
    11461 ctctcctaga ggtgcgagtg tacaagctga taaagcttgt gtttgtcctc gtgtgaacaa
    11521 acctgatcca tgtacacgtg gcaataagct gacctctgat gacaatggac ggatttcatc
    11581 caatttacgc ccgtcaggac ggatcttatc gatggtgatc aattcacgta cgacatcttt
    11641 ttcaagatct tctgtgattt gtttaacttc tttcaacaag tgatcttcat cttcatgacc
    11701 tgcaaatttc tcagcataag cttcgcggat atcgatcttc acctgctcga tattatcttc
    11761 gcgagcaagt ttttcttcag tcattactgc tgctttcatt gtagcgtaag aagcatcaaa
    11821 gatttctttt ttcaatactt catcaacttg cagtaaagtc acttccattt tttctttccc
    11881 tactgcttga acgatttctt cttggaaagc aactaactct ttgatagcgt caaaaccaaa
    11941 cagcaatgca ccaagcatgt cttcttctga aacttctttc gcgccgcttt caaccatatt
    12001 gatggcttgt ttcgttccgg caacagtcaa ttcgatatct gtttgttctg cttgttctac
    12061 tgttggattc aagacgtact cgccatttac acgaccaact tctacgcctg cgattgggcc
    12121 gtcaaatggg atatctgaga ttgatagtgc taaagaagaa cctagcattg ctgccatagc
    12181 tggcgagcag tctgtttcta cactcatcac gatgttcgtt acttggactt cattacggaa
    12241 gccttctgca aacatcgggc gaattggacg gtcgatcaaa cgggcagtca atgttgcttc
    12301 cgtacttgga cggccttctc gtttgatgaa acctccaggg attttccctg ctgcatacat
    12361 tttttcttca taattgatcg ttaatgggaa aaagtctgta tcttttgctt ctttagaggc
    12421 cacagctgca cttaagacta ctgtatctcc ataacggact aaaacggctc cgtttgcttg
    12481 ttttgctagc tgaccgactt cgatttgtaa gggacggccg ccccatgtcg ttttgaatac
    12541 ttgtttttct gacataactt ctccttttcc ttctcgtgaa agcaagacgc tactaaacaa
    12601 atgaaaactg ccgaaagccg tcttttgagc agctttcatt tggttagtag gagtccatct
    12661 agcttgatca cggaatttta cttaattgat atacgagtaa aaaaagtgag attcatgtga
    12721 atcccactct ttttgactaa aattaacgac gcaagcctaa acgtttgatc aattcgcggt
    12781 aacgttgaac gtcagtttta cgtaagtaag ctaacaagtt acggcggtga ccaacttttt
    12841 tcattaggcc gcggtatgaa tgatggtctt ttttgtgcac gcgtgcatgt tcattcaagt
    12901 ggttgatttc ttcagttaat acagcgattt gtacttctgg agaaccagta tccccttcat
    12961 ggcgagcgta ttcgttgatg atttcattct tgcgttcttt tgaaattgcc atttctttca
    13021 cctctttcga ttattcccct gcgactgagt aaaacgttgg tgattcgctt aaccaagtgg
    13081 caggtcatgt gcctagagca cacagctata ctttacctga aaccgggttg aaaatcaagt
    13141 agttaatcat taagaaaagg tagttttcaa acaatcttac tactactttt ccatttttgt
    13201 ggtaaactat aatgcgaaaa tagacttgaa acacagtctt ttaagaaagg ggtacatatt
    13261 ccatgattac aatggatgat atcattcgtg aaggaaatcc gaccttacgc gaagttgcaa
    13321 aagaagtttc tcttccatta agcgaagaag atatctcttt aggaaaagag atgcttgagt
    13381 tcttaaaaaa cagccaagat ccaatcaaag ctgaagaact acacttacga ggcggtgttg
    13441 gcttagctgc tcctcaatta gatatttcta aaagaatcat tgctgttcac gtaccaagtc
    13501 ctgacccaga ggcagatgga ccttctctaa gtactgttat gtataatcct aaaattttga
    13561 gccattctgt tcaagatgct tgtttagggg aaggtgaagg ctgtttatct gttgatcgag
    13621 aagtaccagg atatgttgtt cgccatgcca aaatcactgt gtcttattat gatatgaatg
    13681 gtgaaaaaca caagattcgt ttgaagaatt atgaatcgat cgtcgttcaa catgaaatcg
    13741 accatatcaa cggtgtgatg ttctatgacc acatcaacga tcaaaatcca tttgcactaa
    13801 aagaaggcgt attggtgatc gagtagagaa ttggcaggtt ccttcgatac tatgctacaa
    13861 gaaagcttta taaaaaaggg gagtgctcaa aagctgcact tccctttttt atgtttctcc
    13921 actaatcgaa atttagatag acatctagaa atcgcaattc caaaaaattc tgtgtattgg
    13981 atacgcatct tttcttattt tttcttattt cttccatatg ggtgtttatc tcagagatga
    14041 tagaatagtt tctaggataa aaagtagcag ctacgatgtt atccttcgta gaggattcct
    14101 gcttcagcta gtttttgttt gatgtttttc agcatatgct tttctgggta gcggatcgta
    14161 tgcagatgga tgccgcctgt taattcggat aacaaagtag cattactttt ttgataacgt
    14221 ctcataaagg aatcgatatc ttgtggcgtt ttgatgtgca aagttcctgc taactcaccg
    14281 taaaggggat gttccacaat tacatcaatg atttcaccac cattttcaac gattagtctc
    14341 agttcttctt ctgtttgatc ttttccatgc tggacagcga ttttactgat gtgcccagta
    14401 ttggtttctt ctgtttcgag gatataacct cttgctgttg ccacgatact tttacctgca
    14461 gctcgtagta aagcaatatc gcctacaatg atctgacggc tgactccaaa agttttcgca
    14521 aacttgctag cactcactgg tttttctgta tttgccaagg tttgcatgat ttgttttcgc
    14581 cgtgtctctc cttcgatcac acttattcct ccttcatttt gttttggtat tcgcgcgagt
    14641 gtttcatcaa atcaacatac gtatctgtta ttgtttcctt atatccctta tttttcagca
    14701 gactaacaac tttatccaat acttgctgct ctctttttgc atcataaact gcttggtttt
    14761 cttctttttt cacttgtcct attttagtta ccgtatccat tcttttttct aataaagcaa
    14821 tcaactctcg atcgatcata tcgatcttat gacgtaatgt ttcaagagac tcttctgtta
    14881 gttcttcctc tactgtcttg atgatacgag caggatttcc tgcaatcact acattgtcgg
    14941 gaaaagattt ggtcacaacc gaacctgcgc cgactactac gttgtcccct agagtgacac
    15001 ccggcgtgac gataactcct ccgcctaacc aaacattatt accgatttta atgggttttg
    15061 cgtactccag accgctgtta cgtttcactg gatgtagtgg atgcgtcgcc gtgtaaagct
    15121 ggacattcgg agcgaacatg cagttatcac cgatttcaat cgtgctgaca tctaaaaatg
    15181 tacaattgaa atttgcataa aaattctccc caacaaatat attatatccg taatcaaaat
    15241 tgataactgg ctccatatag atttttttcc ctgtacggcc aaaagtctct ttcaacaatt
    15301 gggatcgcag ttccgctgat tctgcttggt tgatgatttg gctttgactt cttgcaaaca
    15361 tacggtcggc aaccagctca ggatcattgg caaaataaag ctcgcctgca atcatttttt
    15421 ctttttcggt tttgaattca gtcataatct gctcctaatt cttgtatttt tttgaaaaca
    15481 accgctccgt cttcttgaat aaacgtataa ggctccgcta aataaacagg cgtttgattg
    15541 attactaaga ttttggctga cggctggcga aactgtacca gatcacaaaa cggatgaact
    15601 tgaaaactcg ttccaacaat gacgatcagc tctgcttggc tgacggcata agccgcctgc
    15661 tccatgactc catcttgaaa cccttcacca tataacacga tgtctgggcg tatttgtcct
    15721 ccgcatgctt gatgtttgtc gctgtgaaga tattctttcc agtctaccgt ttgttgacat
    15781 tttcgacaat gacattgata caaacttcca tgaaaattga cgagttgcgg gcttttagcc
    15841 tttgcgtgca aaccgtcgat gttttgggaa acaacccaaa catttttttc tttcgctaga
    15901 tgtgccattt ccaaatgaat gatattgggt tttgcttcag gatgataaat ctttttgaca
    15961 aattgataga atttatccgg ttcattcacc attgcttgat gactcagcaa gtattccggc
    16021 tgctccatcc cgtggtagat tcctgacaaa gatcgatagt ctggcactcc agaaggtgtg
    16081 gatacccctg caccggtcaa aaatgtaatt tgctgataag ctaatatctc ttttgctgct
    16141 tcgtctactg ttaattgttt catgttttgt cacctcactt tccattatag cccatactcg
    16201 aacaaattta ttgttgaaaa gtcagaaaaa tgggaataaa aaagtgttat gggcatttga
    16261 acaatttatc acaatgttca ctgacaaaag aatgaaaacg tgttacaata ctgctgtata
    16321 aaagtgaggt gaatcgatat gttaccattc tttatttatt ggggcgttat tctggcttgt
    16381 atcgcctggt tagcgttaag cgtttatttc tctgtttttt atcttgtacg caaagaaaat
    16441 ggaaatcttt gggcttttgc tttattcaac gtgattgccg caattgtttt agccattaca
    16501 ttagccgtat atcgaacatg gggctgggga atcacccaat actcaagttt gatttatcta
    16561 atattagcca tttatggcgt cgttgttctt ttgcaagcga tcctaggacg tgagccaaag
    16621 aaagcggcag cttaaatgat cgagatggaa ctggacacag gaaattttct gttctagttc
    16681 tttttttatt gtattacata catttttgtg tataattata ctatcgtttg caatggatag
    16741 atgattcaca ttttgattgt tttttcggta aaagtaaaga tttctatatt tatcagtatt
    16801 ttttcaaaaa acagcagtta tagattgaca aagcctctcg agtatcgtaa actttttttg
    16861 tataaataac tgaatacgag atgcctcaat caatgaggta gaggtcgcga gaatcattaa
    16921 ccctgtggag tgaagcaaac atggagaagc aggaataggg aggatcgccg aaatgtgcac
    16981 ttttttgctt ggagtgtatg ttgggacgca agttaagagc ttgcggactg tcttagcagt
    17041 gatgctaagt tgcgctatgt tttgtgagag gtttatcacc ttatggaatt ttagcggcct
    17101 ttttatatct ataaatcggt cgctttttat tttttcagat aaagctttac cgcatttcat
    17161 ctagaccgct cgtgtatcat ataatcagaa agggagaaaa aaatgaaaaa aaatatggtt
    17221 tcagttgccg tactatttgc ttttgtcttc tcactattca gtgtgggatc tctggcaaat
    17281 gcagatgaaa aaacaccgag cgatgagttt cgcgtaggta tggaggctgg gtatgcaccc
    17341 ttcaactggt ctcaacaaac agatgagaat aatgccttgc caattcaagg ccaaaatagt
    17401 tacgccggcg gttatgatgt tcaaatcgct aaaaaagtag cagatggttt agggaaaaaa
    17461 ttagtcatcg tccaaacgaa atgggatggt ctggctcctg cccttcaatc aggcaagatc
    17521 gacgcgatca tcgccggaat gagccctact gctgaacgac gaaaagaaat tgattttacc
    17581 gatccttatt acgaatcaca attagtagta gtagttcaaa aagatagcaa atatgcaaaa
    17641 gcaaaaagtt tagcagatct ttctggagca aagattactg ctcagttaaa tacattccac
    17701 tatactgtga tcgatcagat accagatgtg cagaaacaac aagcaatgga taatttctca
    17761 gcaatgagaa cagctttagc ttctggaatg atcgatggtt atgtcagcga acgtccggaa
    17821 ggtgtaacgg caactagtgt gaacaaagac ttggaaatgc ttgaattcag cggtgcagat
    17881 ggcttccaga caaacccgga agatgttcag atttctgttg gtatgcgtaa aggcgacccg
    17941 gatatccaaa aagtaaacca gattctttct ggcatctcta acgatgaacg aaccaaaatt
    18001 atggaccaag cagtcaaaga tcagcctgct gcaactgata gtgaagaaaa acctggcatc
    18061 atttctgatt tcactacgat ttggaaacaa tacggcaata tgttcttacg aggtgctgga
    18121 cttaccttgt tcatcgcatt gatcgggaca gttgtcggta ctacacttgg attactgatt
    18181 ggtgtcttcc gttcggtacc tgaatcagga aatgcactga ctcgcttctt ccaaaaaatc
    18241 ggaaatgcac ttctttctat ctatgtcgaa gtattccgcg gaacaccgat gatggtacaa
    18301 gcaatggtca tcttctacgg tctggcactc gcttttggca tttcattgaa ccgaacgatt
    18361 gccgctttgt ttattgtctc tctcaatact ggagcttaca tgacagagat cgttcgtggg
    18421 gggatttttg cagtagatga agggcagttc gaagctgcgc aagccattgg tatgacccat
    18481 ggccaaacga tgagaaaagt tgttattcca caagttttac ggaatatctt gccagcaaca
    18541 ggaaatgagt ttgttatcaa tatcaaagat acagcagttt taagtgtaat cggcgtagct
    18601 gatttgttct tccaaggaaa ctctgcttcc ggtgcgaatt tccaattctt ccaaacattt
    18661 acgatcgtcg ggatcatcta cttagtcatg acctttacga tcaccagaat cctgcgggcg
    18721 gtagaaaaga aaatcgatgg tccttcagct tataccaagc ttgaagaaat cgacaattca
    18781 aatttgcagg aataaggagg aagaatcatg aatgaaaaaa ttattgaaat caaacatcta
    18841 agaaaaagct tcggtgaaaa tgaagtattg aaagatatca gcgtaacagt caataaaggt
    18901 gaagttgtga cgatcatcgg ttcttccggt tctggtaaat ctactttctt gcgctgtatc
    18961 aatttactgg aaaaaccaac aggcggcgag atcatttata atggagaaaa tgtattagct
    19021 cctaaatata atttacctaa atatcgaacg aatctaggta tggttttcca atccttcaac
    19081 ctgttcaaca atatgaatgt cttggaaaac tgtatgtctg gtcaaacaac tgtcctgaaa
    19141 cgagataaag aaactgccaa aaaagtagca atcgaaaatc tggaaaaagt tggtatggat
    19201 cgttatatcg aagccaaacc agcacaatta tctggtggac agaaacagcg tgtcgcaatt
    19261 gctcgggcat tatcaatgaa tccagatgta ttgctattcg acgaacctac ttcggcgtta
    19321 gaccctgaaa tggttggcga agtgcttaat acgatcaaag acttagcaca tactggcttg
    19381 acaatgctta tcgtgacgca tgagatggaa tttgcaaaag atgtatctga ccgtgtcatt
    19441 tttatggata aaggtgtgat tgcagaagaa ggtacaccag aagatatctt tgtccatccg
    19501 aaagaagaac gtacaaaaga attcctttca cgtattttaa atgcttagga tcaatagctg
    19561 attgggacaa agagctgcgg tgtttaccgc agttcttttt tatgaattac tcataaaagg
    19621 taaatttctg aaaattttgg aatcttagtt gtgtttttca gaaaactctt ttaatatgga
    19681 agtaattaaa cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc
    19741 gtggactgaa aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt
    19801 tgtttttagg ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc
    19861 ttattactgg atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa
    19921 acaccaataa ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt
    19981 tcatcactgg ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag
    20041 ccatcggaat gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga
    20101 ttgcattagg tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag
    20161 agttttggtt tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt
    20221 atatgatcct cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt
    20281 ctcatggcgg attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa
    20341 cctttgcttt tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg
    20401 aaaaagtgtt gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag
    20461 gatctctttt tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc
    20521 catttgtaga agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag
    20581 tcgtattaag tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc
    20641 ttcgttcttt agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc
    20701 atgtgccagg gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga
    20761 attacgtgat gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct
    20821 tgtttatctg gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca
    20881 aaaaagctca ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt
    20941 acatggcctt tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac
    21001 taacacctgt gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga
    21061 agtaagaaag tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa
    21121 aattgcttct taaatttttg tgaaatttcg gcttatttct tggagttaaa agcttctgtc
    21181 ccagcctctt ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata
    21241 ggaaaaaatc caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa
    21301 aaagacaccg gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt
    21361 tgacaaaact ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa
    21421 gataccaaaa gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa
    21481 agcttcaggg ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag
    21541 caaaaagata ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg
    21601 atgatggttc acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt
    21661 atttcggatg acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac
    21721 aagaactctc aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt
    21781 cacgatgatt cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata
    21841 accgtcgttt tatataggaa attttctgaa aattgttgca tatttttcga aattttgtta
    21901 cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc
    21961 tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg
    22021 ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta
    22081 ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg
    22141 tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt
    22201 acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg
    22261 gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg
    22321 tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg
    22381 gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt
    22441 tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt
    22501 ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg
    22561 ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta
    22621 aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc
    22681 tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca
    22741 cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta
    22801 tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat
    22861 tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc
    22921 cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga
    22981 tgttcatcta tccattagca atgacactga ttttactagt gttagttggt cctttgttta
    23041 agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg
    23101 atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat
    23161 atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag
    23221 ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt
    23281 gataaaagag gttgtgaaag agacgttcag ccttattgcc gataggctgt ctcttgaaca
    23341 ctgtttattg agaggtcgtg aaaaagccgc tcagtttcga gtattaagaa atattttttg
    23401 aaaatggctt ctcacatttt tgcataaatt tgacttaata ccgagaaact ggcttttgaa
    23461 caccgtttat tgagcgtcta taaactgacc tgagtattag agaaacaaaa cggcaaagta
    23521 gctccatata ctttgccgtt ttgttttgaa ctaataccga agagaagctg atcaaacaac
    23581 atttctttta taatatagat tttagacata tatatgatat aaaaatagtt ttcgcaaaaa
    23641 aa
//
LOCUS       DAZKOB010000037        22823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000037 DAZKOB010000000
VERSION     DAZKOB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.037"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACG8M6_001388"
     CDS             3..371
                     /locus_tag="ACG8M6_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0457065.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACG8M6_001389"
     CDS             551..1492
                     /locus_tag="ACG8M6_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0457066.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACG8M6_001390"
     CDS             1561..2292
                     /locus_tag="ACG8M6_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0457067.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACG8M6_001391"
     CDS             2436..4616
                     /locus_tag="ACG8M6_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0457068.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACG8M6_001392"
     CDS             4636..5118
                     /locus_tag="ACG8M6_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0457069.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACG8M6_001393"
     CDS             5134..5895
                     /locus_tag="ACG8M6_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0457070.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACG8M6_001394"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACG8M6_001394"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0457071.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACG8M6_001395"
     CDS             7667..8152
                     /locus_tag="ACG8M6_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0457072.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACG8M6_001396"
     CDS             8145..8828
                     /locus_tag="ACG8M6_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0457073.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACG8M6_001397"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACG8M6_001397"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0457074.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACG8M6_001398"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACG8M6_001398"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0457075.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACG8M6_001399"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACG8M6_001399"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0457076.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACG8M6_001400"
     CDS             13125..13457
                     /locus_tag="ACG8M6_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0457077.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG8M6_001401"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG8M6_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0457078.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACG8M6_001402"
     CDS             14283..15656
                     /locus_tag="ACG8M6_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0457079.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG8M6_001403"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG8M6_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0457080.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACG8M6_001404"
     CDS             16711..17151
                     /locus_tag="ACG8M6_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0457081.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="ACG8M6_001405"
     CDS             17295..17996
                     /locus_tag="ACG8M6_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0457082.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="ACG8M6_001406"
     CDS             17993..19114
                     /locus_tag="ACG8M6_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0457083.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="ACG8M6_001407"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="ACG8M6_001407"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0457084.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="ACG8M6_001408"
     CDS             20493..21401
                     /locus_tag="ACG8M6_001408"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0457085.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="ACG8M6_001409"
     CDS             21436..21708
                     /locus_tag="ACG8M6_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0457086.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="ACG8M6_001410"
     CDS             21719..22765
                     /locus_tag="ACG8M6_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0457087.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7697 a         3981 c         5011 g         6134 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    22801 agaaaaacgt tatggataag ata
//
LOCUS       DAZKOB010000038        22719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000038 DAZKOB010000000
VERSION     DAZKOB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.038"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACG8M6_001411"
     CDS             complement(11..1942)
                     /locus_tag="ACG8M6_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0457088.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG8M6_001412"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG8M6_001412"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0457089.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACG8M6_001413"
     CDS             complement(2871..3623)
                     /locus_tag="ACG8M6_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0457090.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACG8M6_001414"
     CDS             complement(3782..3991)
                     /locus_tag="ACG8M6_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457091.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACG8M6_001415"
     CDS             complement(4002..5330)
                     /locus_tag="ACG8M6_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457092.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACG8M6_001416"
     CDS             5466..5621
                     /locus_tag="ACG8M6_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0457093.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG8M6_001417"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG8M6_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0457094.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG8M6_001418"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG8M6_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0457095.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG8M6_001419"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG8M6_001419"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0457096.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACG8M6_001420"
     CDS             complement(9305..10237)
                     /locus_tag="ACG8M6_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457097.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACG8M6_001421"
     CDS             complement(10394..11053)
                     /locus_tag="ACG8M6_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0457098.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG8M6_001422"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG8M6_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0457099.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACG8M6_001423"
     CDS             complement(12094..12384)
                     /locus_tag="ACG8M6_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457100.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACG8M6_001424"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACG8M6_001424"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0457101.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG8M6_001425"
     CDS             complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG8M6_001425"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0457102.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="ACG8M6_001426"
     CDS             complement(16049..17218)
                     /locus_tag="ACG8M6_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0457103.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG8M6_001427"
     CDS             complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG8M6_001427"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0457104.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG8M6_001428"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG8M6_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0457105.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="ACG8M6_001429"
     CDS             22182..22703
                     /locus_tag="ACG8M6_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0457106.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6326 a         4932 c         4041 g         7420 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata aggagcgtg
//
LOCUS       DAZKOB010000039        22440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000039 DAZKOB010000000
VERSION     DAZKOB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.039"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(260..712)
                     /locus_tag="ACG8M6_001430"
     CDS             complement(260..712)
                     /locus_tag="ACG8M6_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0457107.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(725..1135)
                     /locus_tag="ACG8M6_001431"
     CDS             complement(725..1135)
                     /locus_tag="ACG8M6_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0457108.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1410..1622
                     /locus_tag="ACG8M6_001432"
     CDS             1410..1622
                     /locus_tag="ACG8M6_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0457109.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1625..1837
                     /locus_tag="ACG8M6_001433"
     CDS             1625..1837
                     /locus_tag="ACG8M6_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457110.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            1977..2132
                     /locus_tag="ACG8M6_001434"
     CDS             1977..2132
                     /locus_tag="ACG8M6_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457111.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2129..2353)
                     /locus_tag="ACG8M6_001435"
     CDS             complement(2129..2353)
                     /locus_tag="ACG8M6_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0457112.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2431..2619
                     /locus_tag="ACG8M6_001436"
     CDS             2431..2619
                     /locus_tag="ACG8M6_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457113.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2616..2789
                     /locus_tag="ACG8M6_001437"
     CDS             2616..2789
                     /locus_tag="ACG8M6_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457114.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            2758..2886
                     /locus_tag="ACG8M6_001438"
     CDS             2758..2886
                     /locus_tag="ACG8M6_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457115.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2945..3280
                     /locus_tag="ACG8M6_001439"
     CDS             2945..3280
                     /locus_tag="ACG8M6_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457116.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3513..4454
                     /locus_tag="ACG8M6_001440"
     CDS             3513..4454
                     /locus_tag="ACG8M6_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0457117.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4456..5346
                     /locus_tag="ACG8M6_001441"
     CDS             4456..5346
                     /locus_tag="ACG8M6_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0457118.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5389..6189
                     /locus_tag="ACG8M6_001442"
     CDS             5389..6189
                     /locus_tag="ACG8M6_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0457119.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6204..7055
                     /locus_tag="ACG8M6_001443"
     CDS             6204..7055
                     /locus_tag="ACG8M6_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0457120.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7121..7255
                     /locus_tag="ACG8M6_001444"
     CDS             7121..7255
                     /locus_tag="ACG8M6_001444"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457121.1"
                     /translation="MDELSQQESRMVRKISKLIIYRDTERFLTVLGNVYENPELLEVE
                     "
     gene            7256..7717
                     /locus_tag="ACG8M6_001445"
     CDS             7256..7717
                     /locus_tag="ACG8M6_001445"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0457122.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7743..8195
                     /locus_tag="ACG8M6_001446"
     CDS             7743..8195
                     /locus_tag="ACG8M6_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457123.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8301..8495
                     /locus_tag="ACG8M6_001447"
     CDS             8301..8495
                     /locus_tag="ACG8M6_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457124.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8526..8819
                     /locus_tag="ACG8M6_001448"
     CDS             8526..8819
                     /locus_tag="ACG8M6_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457125.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8750..9289
                     /locus_tag="ACG8M6_001449"
     CDS             8750..9289
                     /locus_tag="ACG8M6_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0457126.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            9364..9831
                     /locus_tag="ACG8M6_001450"
     CDS             9364..9831
                     /locus_tag="ACG8M6_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0457127.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10578..10808
                     /locus_tag="ACG8M6_001451"
     CDS             10578..10808
                     /locus_tag="ACG8M6_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457128.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            10967..11800
                     /gene="terS"
                     /locus_tag="ACG8M6_001452"
     CDS             10967..11800
                     /gene="terS"
                     /locus_tag="ACG8M6_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0457129.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11793..13208
                     /locus_tag="ACG8M6_001453"
     CDS             11793..13208
                     /locus_tag="ACG8M6_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0457130.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13220..14749
                     /locus_tag="ACG8M6_001454"
     CDS             13220..14749
                     /locus_tag="ACG8M6_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0457131.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14667..15719
                     /locus_tag="ACG8M6_001455"
     CDS             14667..15719
                     /locus_tag="ACG8M6_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0457132.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15721..16038
                     /locus_tag="ACG8M6_001456"
     CDS             15721..16038
                     /locus_tag="ACG8M6_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457133.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16165..16788
                     /locus_tag="ACG8M6_001457"
     CDS             16165..16788
                     /locus_tag="ACG8M6_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0457134.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16803..17693
                     /locus_tag="ACG8M6_001458"
     CDS             16803..17693
                     /locus_tag="ACG8M6_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0457135.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17716..17931
                     /locus_tag="ACG8M6_001459"
     CDS             17716..17931
                     /locus_tag="ACG8M6_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457136.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            17943..18284
                     /locus_tag="ACG8M6_001460"
     CDS             17943..18284
                     /locus_tag="ACG8M6_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457137.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18284..18655
                     /locus_tag="ACG8M6_001461"
     CDS             18284..18655
                     /locus_tag="ACG8M6_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457138.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18648..19043
                     /locus_tag="ACG8M6_001462"
     CDS             18648..19043
                     /locus_tag="ACG8M6_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0457139.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19045..19422
                     /locus_tag="ACG8M6_001463"
     CDS             19045..19422
                     /locus_tag="ACG8M6_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0457140.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19423..20031
                     /locus_tag="ACG8M6_001464"
     CDS             19423..20031
                     /locus_tag="ACG8M6_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0457141.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20031..20372
                     /locus_tag="ACG8M6_001465"
     CDS             20031..20372
                     /locus_tag="ACG8M6_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457142.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20614..>22440
                     /locus_tag="ACG8M6_001466"
     CDS             20614..>22440
                     /locus_tag="ACG8M6_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0457143.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNI"
BASE COUNT         8049 a         3301 c         4603 g         6487 t
ORIGIN      
        1 ctgatagtgt ttcttcctta cttaatataa atctttctct atcatgtaat ttatttagtt
       61 tttcatcatt tttttgttct aattcataag cttctttttt tattgtgtta gctttcgaca
      121 gtttagtgat tcctataatt aataaaataa gtccaatcaa taataacatt caaaactcct
      181 catttctttg atataattat tgtagagttt actcagaaat gaggaaagag tccatgtgaa
      241 agcatgggct tttttatttt taatgttgta atactaaatg ttcaagatta tgaggaagcc
      301 ctaaatattc aataatttgg aactgactca tatcagataa atcttgttca tctattgttc
      361 tagttaacat ttcaattgca aacatattag cttcagcttc tataccataa atcatacaac
      421 taaggttgtt tgctctaaaa gctgctgtgc aaataccttt atggagtcta acgtgggcta
      481 tttcatgcca taatacaaat cttttcatag aaggtgatag gttttgatta attaatatca
      541 cataacatct tttattgtgt atcgtttgtc ctaatgtttt attccctaaa tcagtttcaa
      601 ttaaatctat atcagaatat ttgattattt tatatggatt aatcgcatca gatccaaact
      661 ctacaaataa tttttctatt tcttctaata tccagtattt gtttaaatgc ataccaaagc
      721 ccacctattg acgatatttt ttaggagtga attttttctt tgctttaact ttggttaagg
      781 caagtgcaag ttgaatggca tcgtataatt tttccttatc ttcatcgtct aaaggttcac
      841 cataataatt tgtttcagca tctgaattca agccttcaag taatttttca gcttgtaaag
      901 caatatcttg tttatctttt tctgttaaat cataataatg gcgtttatca gtacgtccta
      961 aaagataatc agttgataca ttaaagtaat ctgcaacttt ttgtatttta tctccagatg
     1021 gagtactttt attccatcta gacatgcttc catttccgaa atttaacttt ctttcaagtt
     1081 ctgccaacgt cattgaacgt tcttttaata agatttttat acggtctaat agcataggat
     1141 atgcattcct ttcaaaatac cgacaaaaaa agtatttttc tgcaaattat ctatttacaa
     1201 atagagaatt tgcagttata ctagttttgt aagcaagata tttacggcat acaaagacta
     1261 aaagcaataa actaatagtc tccccagaca tcagtaagct cgctttttgt gcgctgtatt
     1321 ttgtatgctt acataatagc atatttgcag tttttgtcaa acttattaga caaaaatatt
     1381 agaaaatatt cgataagggg gtatgaagca tgactttaaa acagcaagta ttaattgctt
     1441 tagtaaagaa aggttggagt caaagagagt tagctcgacg aatgggtata tcaattactt
     1501 atttacgaga tatcttcatc gaaaaaagaa aacctaagga acgtttaaaa caaatcgaag
     1561 aacttttgga tatcaagtta gaagtagatt ctaatgatca gccatcagaa caggaggcgt
     1621 aaaaatggta caacgtttga gcaaagcgga gtttaaagag aaatataagt attctgaaag
     1681 tacttatcaa agaagaatga aggaatttaa aaaatcacgt tttagtgagg ggtataacgc
     1741 agttacttcc aaagaaataa caattgatgt cgatttatat gaacagttta gaacatggcg
     1801 ttctaaaaat cgatttagag ttagagctgt ttgttaatag ctaaaaaatc taaattcacc
     1861 acttctgaaa agaggagttg ctgctctcct ttcaagtttg ccaaagtcca cgttttttca
     1921 ttttggcaac tcctcttctt ggaggtggtg acggcgagag acgaaggagg gatgacatga
     1981 aggaagaatc aaaaataggg attttgaatg agaacaatta cacaagatta attattgaaa
     2041 cagaagaagg gaaaaaagta gcagaagtta cattgaccga tgcaactcct gctgacggtt
     2101 atcgtattcg attaactcct aaatatgatt aaccttttgg tgggtgtgga tcgtttccgt
     2161 gactatcacg gctttgaatt tttccatctc tgccatgaat aaatagctca gatccttgat
     2221 ttttagaaat ctctctagcg atttttactg cttctgattt tgtattagta tgtgcagtcg
     2281 ctttagaatt tccagcacct ttgacattcc aaccaccatt ggaattaggt acaacatgtt
     2341 gattttttcc catagttttc acctccattt ctatagaaat attttatcaa atagaaatgg
     2401 agaaattcaa taaacgaaag gagaagtgat atggcatata ccttgcaaca agaacatcaa
     2461 attctcggtt tgattaaaca acgcaggaaa caattacaag atgaccgtgc agcgcttaga
     2521 aaagccgatg agctatcaga taggcaagct gaactaattg cttctgaact tgaggatttg
     2581 agaatgctag aaataaaaaa tagggagatt agattatgaa gaagacagac acacttttta
     2641 taggattcat tttggggtta ttagtgattg tagcgcacca aagtattatc gggggaagct
     2701 tgttcgcagc attgatggtt ttaatcaatc tgcttgattc aaaagaaagg agcaactatg
     2761 gcacgagaag aagcgctaaa aatcggtaaa gtgattgctg ataatcggtg gggaaatagc
     2821 cgccctatta ttttaagcaa gcaacatatc gaaaagcaaa aagcatggca acaaataaaa
     2881 aagtgactct gccagccagc aaagagtcac atacaaattt ataaaactag gagaattata
     2941 tcacatgaat aagaaaattg aaaatcttat cgaagaattg aaacgtgaat gccaaaagca
     3001 aggtgtttct attatttgta cagcccaaaa agaaggggaa cttaaaagtc tcgtttatgg
     3061 tgagacaaca gaaattttac tttgtcttgc tatgcaagaa gaacatttag atgaaaatct
     3121 cccattgtca gcacatatta tgcgtagaat tgctgtggat gcttacgaac aagcgaaaaa
     3181 tgaagaagaa aatcaacctt caaatcatac ttttgttatt aataacaagg aagatttagc
     3241 tgatgtgatg actcgtattc tcaaggggga atttcaatga tgaatgaaaa aacaattgat
     3301 ctactcatta ttttattttt cacgttagtg attgtagcga aaagttatgt aatctttggc
     3361 ttgttattcg caattaccat gcttgtgctt tgcgtgttag caagcagaaa agaggttaaa
     3421 catgaacgag ttagcaaatc ttgacaacta tttaactgat cctgattatg ctaaaccacc
     3481 atatgaagca ccaattgatg aggaggatga agatgagtaa atctacctta gaaatgagcc
     3541 atcaagaatg gcttgaagac cgtaagaaag gcatcggagg ttcggatgtt ggaacaattt
     3601 taggattgaa taaatggaaa tctccttatc aactatggct agaaaaaaca ggacaagtag
     3661 tacttgaaga atcaggaagt gagcccgctt attggggtaa tgttttagaa gaagtggttg
     3721 ctaaagagtt tcaggaacgt acaggcaaaa aagtacgcag aagaaaccaa gtatttgaac
     3781 atccattaca tccattttta agagcaaata ttgatcggga agtagtggga gaaaatgcca
     3841 ttctggaatg caaaacagcc aatcaatttc tcggtaaaga atgggaaggc gaagaggtac
     3901 cgcttagcta tctctgtcaa gttcaacatt acatgaacgt tctaaacaaa gactattgtt
     3961 acatcgctgt cttaatcggt ggtcaaaaat ttatctggaa gcggattgaa cgagatcaag
     4021 agctgatcga tacaatcact gaacaattag tagagttttg ggaaacaaac gttcttggag
     4081 gtatcgagcc aattattgat ggtagtcaag cgacagctga tttcttgaaa gagaagtatg
     4141 cagatgttga agacgttcaa acgacgttac caattcattt cgatgaacta gttgaacaga
     4201 aaaacgaact caaacggact aagaaagaaa ttgaatcagc tatccgtcaa gtagacaacg
     4261 agattatcag cgagctaggt aaacgtgaag ccagtatcgg tatcactcaa aaaaacatca
     4321 tcagttggaa atttgtccgt acgagacgta tgaactcgaa gaaactagca gagaaatatc
     4381 cagatgtcgc aaatgatgaa gagatttata acgttactga atcaagaagg ctaaccgaaa
     4441 gggagatcaa ataatatggc aacaaatgaa tcgttaaaaa atcaattggc agcaaagcca
     4501 cagaaacaag ttgcaccagg tcaattagga cttaaagctt tgatgaatac accaacaatg
     4561 agaaagaagt tcgaagaggt gcttcatgac aatgctaatg cttttatgtc gaatgttatg
     4621 actcttgtat ctaatgacag ttatcttgca gatagtgaac cgatgtctat catgagtggt
     4681 gcgttaactg ctgcaacatt aaatcttggg ctagataaga atttaggtta tgcatattta
     4741 gttccattca atagtaaaaa caagcaaaca ggaaaatggg aaaagaaagc tcaattcatg
     4801 cttggctata aaggatatat ccaattagcc caacgatcag gtaaatacaa agcattaaat
     4861 gtgatcgaag tttacgaagg agaactaaaa agctggaacc gactgacaga agagtttgag
     4921 tttgatccaa atggtagaac gtctgatgaa gtcattggat atgttggcta tttcgagtta
     4981 ctgaatggat tcaaaaaaac tgtctattgg accaaacaag aaatcgaagc tcatcggatt
     5041 gctaacaata aagatcgcga taagacaaag ttaagtggtg tgtgggcatc tgattacaat
     5101 gcaatggcac gaaaaactgt tttgagaaat cttctttcta aatggggaat cttgtccatc
     5161 gaaatgcaag aagccaccac atcggatgag agagtccaaa gggttcaaga agacggcagc
     5221 attattgctg aaacagaagt tgaagaagat attcctgaaa gaaaagaagc agaggttata
     5281 tctgaagaaa atgaagatgt acaaactgga ttatttgatg catctaatcc gccgttaaac
     5341 aaataatgag ggagctttct cccttgcttt tctagaggag aaatacgaat gaacacagga
     5401 tatgtcaaat tacatcgaaa agtgatgaat tcattcgttt ggaccaatcc ttatatgtat
     5461 aaattatgga acttgtgttt gatgaaagcg agtcatgaga atcgcaagat actttttaat
     5521 ggaaaagaga tacaagtgaa cagcggagaa ttcgtcacag ggcgcgatgc tatcacatct
     5581 gagatgaacg aaggcgtgaa acgtgaacat caagtgaaca gcggttctgt atggagatgg
     5641 ctaaaacaat ttgaaaaaaa cggaatgttg aacatcaaat caactacgaa atacagcgtt
     5701 atatcaataa aaaactggtc tttgtaccaa agcagtgaac aacaaatgaa catcaaacgc
     5761 acaacaagtg aacaacaagt gcacacaaac aagaatgaaa agaattataa gaatgaaaag
     5821 aataataaaa catcatcgtc gcaaccacgc aaaaagcgtg tttacgacac cgactcagtt
     5881 tactacattc tcgcggagga gttattcaaa cagatttgtc agaatcagga aatcaaaaag
     5941 ccgaatctgc aaagttgggc agatgacatt cggaaaatga tcgagatcga caagcggacc
     6001 gaaaaccagg tacggggaat gattgaatgg agtcagcaca atgtgttttg ggcatcgaat
     6061 attttatctg ctaaaaaatt gcgagaaaaa tacgacacaa tggcagcgca agcaaatcgt
     6121 gattataaaa caaaacaaac taaaacgctt gaatacgaga aatttagcac agatgagttg
     6181 cctatttgag aggaggcgta ggcatggaaa ctgttgggga aatcatggaa aagctgatac
     6241 agaaagtgct tgtccaacgt ggcgaatgtc ctgaatgcgg acagcctttg tatggatggc
     6301 gtacgaagaa ccctgatggc tcagaacgtt gtaagccaac ctgtatgcaa tgtgggtata
     6361 aagcattgcg agttcaggaa gacttacaaa cagaacgaat ttacaacgag agcctgaaag
     6421 caagagcaat caattttttc aaaggtggtt ctgttgtgcc taatcaagcg ttgtttgatt
     6481 gcacattgca gaattatcaa attgtcgatc aagaaacaag acaagcggtt gaagtaacca
     6541 aacgctttgt taattcagtc ttgttaggaa atccaagtca ccttgtttta actggaaaac
     6601 aaggaacagg taaaagtcac ctagcgatgg cagcggcttg ggaagtcttg aagcgatcaa
     6661 actacgataa aaaaatctta tttatcgggt tacaggaaat gctggatcaa atcaaatttt
     6721 cgtacaacaa tcctgaactc agaaaaacga ttgagggatc gttgattgca gatatcaaaa
     6781 cagcggattt agtcatcatt gatgatatcg gttcagaact aggaaaagat gcatcagata
     6841 gtcgagcgtt tggcataaac acgctaaatt cgttcttgga tgcacgacag aacctagcaa
     6901 cgattatcac aacgaacttg cttggtgaag aactgaaaaa agcttacggt acgagaacaa
     6961 tatcaagaat gtttgtcaac tctgatggat ttacgatggt attttctcaa acagcagaca
     7021 agcgcataaa accagtcaaa ggagcgatcg catgaatttg actaaacaag aaaaaagaat
     7081 ctgtcgtcag ataatgttgc aaattatagg tgatcgtgac atggacgaat tatctcaaca
     7141 agagagtcga atggttcgga agataagtaa gctaattatt tatcgggata cagaaagatt
     7201 tctcactgtt ttaggtaacg tctacgagaa tccagagtta ttggaggtag agtaattgcc
     7261 aaaaatatta gatgcatgtt gcggtagtag attgttctgg tttgataaga acaattctga
     7321 cgtaacattt atggattgtc gtcagcagta tgaagaatta tcaacaggtc atgtgatcaa
     7381 tgttgatcca gatgtggtcg cagactttcg cgatatgcct tttgaagatg atgtgttcga
     7441 tatggttgta tttgacccgc cacacttaag acatgtcggc gaaaactcat ggcttgccaa
     7501 aaaatatgga aaattagacg aactttggcc agaagacatc aggcaaggtt ttgcagagtg
     7561 tatgcgagtt ttacgacctt caggaacatt aatttttaag tggaacgagg aacagatacc
     7621 actttctgat gtgttagagg ctattggaga acagccattg tttggtaata aacgaagcaa
     7681 aacacattgg cttgtgttta tgaaaaaatt cagctaacgg aggaaacaaa ggaggaaaag
     7741 taatgaagat tgtattaaat aaatgttttg gtggctttgg gttgagccat gaagcaaaaa
     7801 tggaaatttt taaaagaaaa aatattgaag tattcccata catcaataac tttagctatg
     7861 actcagatga tgaatacacg agatacacag gccaaaagct aggttcgatg gattttatct
     7921 actatttcaa aaaagacccg aaaatcgaca aagttacagg aatttatagc gaaattgacc
     7981 gactgtacgg aattgctgat gattccagtt tttcaagtga ttcaaacaga ggtgataagg
     8041 acctcgttgc tgttgtcgaa aaactaggaa gtgaggccag tggaccatat gcaagtctta
     8101 aagtcgttga tatacccgat ggggctgagt gggaaataag tgattatgac ggcgttgaaa
     8161 ctgcacatta tggttttcaa acaggtagta tttagtccac taatgacaac taaagaggag
     8221 gcagaaggat gagtgaagat gaatactttg aacagtagtc tattaacgac agagattttt
     8281 aactggaaag gggtgtagtc atggacgcat cggaagtagt aaataaaatt aatagtctca
     8341 aagaggtttt cggagacgtc gaagtagtga taaacgcaca aggctatgac aacgaatttt
     8401 ttaagcagat taccgacatt agcattcagc aaggaatgga agacgaagac ggaaacttta
     8461 tcgatgaagt agccatttta gtgacttgcg agtaagtaat tgacagctaa agaggaggaa
     8521 gcggaatgaa actaaaagac ggattttact ccagcggcca cggcatcggc ggtttaatgc
     8581 tagatatgcc gacaaagaat cctaaaacac gtaagaaacc aaaattcaaa gtcggtgaca
     8641 tggttcgctg cgaagcagaa ggattcatct atccatttcg tggatatgta gaacacgtct
     8701 ataatcactc agcaatcatt cgcattgaaa acacgatgga atgtgataag tggttagcga
     8761 aaagcaaaga gaatttagca gtggtgagat tggtggacat ggaagttata aaatcttaat
     8821 tttgcttttt tacaactatc ctacaacaat gagcgttatt ccacaactgg aaggagaaga
     8881 ggcaattgtg aacattttag agaatataga tattaagcaa acaaggaaaa atgctagacg
     8941 attactaaaa agatacagaa atttagaacg tttggtcgga ccagtgaaaa tagacttttc
     9001 cgtgatgact gttaccaaaa atttgaaatt cacaattgac agtcaaaacg aagaaatcat
     9061 tgaagcgata agtactcgag attcggttat cgaagcatta acacggctaa gtagaatcca
     9121 tttccaggta ctttattata gttattgttt tcctaataag atgtcaatgt atcaaatagg
     9181 agaaaaatta ggatattccg atagaactat cgagagaatg aaggcagtag ctttggttga
     9241 atttgctgag gcgtataaat caggagaact catttcacgt acaaaataaa aaaagtcgga
     9301 ttcctccgac tatgaataat atttccgaca taagtattat atcataattg ggggaatcag
     9361 aggatggtac ttttcgatgt aaagaaatat gagacaccaa gcgcgaaaga tgttgatatg
     9421 gaacgcacaa aacataatgt cgctgtgttt ctttcagcat atctatcagc tagatgtaga
     9481 gtagggcaac atcgagaacc aaaagtgaca gcatcttatt ccttggttcc accttctaca
     9541 gctgatcata tatttgaagc agaaagaatg ctgatcgata aagaagaagc acaggaagaa
     9601 tttgagtatc tgcataaatt gtttgttcga ggctattcag cgatacagca tccgcataag
     9661 cctgatgtga ctgaaaggcg caagaagata ttctatgatc gttatatcaa tggtctgccc
     9721 atttatgtaa ctgctcaaag gaataatact agcgaagaat cggttaaagt agaatcaaac
     9781 agaattatca tccaatttgc ttcatcgtta gaactggttg ctttcaagta gccagttttt
     9841 acacttttta taccctttta ttaccaattt ggtttccatt ttataccttt tttataccaa
     9901 tcacttacct attcaacgtt gtattatgat agtgtcgaaa aattaggaaa caggatcgac
     9961 aaaataaaat gtaagggagg aaatctccct catcgtttaa ttaagcttcg ataggcagca
    10021 acggaaatat taagaataag gatgtgaatt tcaactcctt ctaaattgtt cttattatct
    10081 atcatccgtt gctgtctatt gtttttttaa ttattcacac gataactaaa ggtaagtgaa
    10141 gagaaatgat tccattaata atttcaattt ttgcgctttg tcttaatgta tatatgattg
    10201 gatttaaaaa tgggcaaaat aaaaaatagt agcagccaag aataatttta tagtgtcact
    10261 gtggcggaaa gggtagacgc taagcatgtg tgctaggtca atgcttcggc aaccatgcaa
    10321 ggttcgattc cttgccagtg actttggcaa ccgaggcatc ggcggtttaa aaatataggg
    10381 gtgcgcaatt tcgtacgcgt tttgtgcatc gtgtaggttg ctattacata actggttagg
    10441 ttagattgag atttgggatt cggtacaaat gaatcgtcaa atgtctcaag cacaggatcg
    10501 gaaacgtccc tgcctgtgca ttacatatta gatcactctt tgagtggtct ttttctttac
    10561 ataaaggagg ctacataatg agaaactact ggtatgtatc gctaactaat gaatatcctc
    10621 gaaccattga tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact
    10681 ctattgtcga aatggggaga gaagcagaac catgtgagat tgatgcgtgc aagctcgttt
    10741 attgcggtca tggtttctat gatgaaccaa acattcaaaa taatattaac aagaatttga
    10801 gggattagaa tgcaagaaat ggcttaaaaa tctccaatca ggacaaatca agttagacaa
    10861 agtttccgat ttaaagatat tgattgaagc tgatttaatg ctaaaagata ttgaaaatta
    10921 gaaaacaaaa ctcaacctaa acagaatgtg aggtggtgtg tattgaatgg caagacaacg
    10981 tgatccaaga cgtgatgaag ccaaaagaat ttggttagaa tccaacggag aaaagcagtt
    11041 aaaggaaatt gcatctgaat taaatgtttc agattctcag gttagaaaat ggaaatcgca
    11101 agacaaatgg agcgctgaat tgattagtaa cgttaccaat ggcaaaagta acgttactaa
    11161 tcaaggtggc gctcctattg gtaatcaaaa tgctaaaggt aacaaaggaa atagccgagc
    11221 ttctccgcca gtgggtaata aaaacgcttt gaaaacaggt gagtatgaaa caatattttt
    11281 tgacacgtta accgataatg agaaggacat ctattctagt ttgaatgatg atccttcttt
    11341 tgctttgtcc gaagaaatac gacttcttaa aataagacaa cttcgaatga tgaaaagaat
    11401 ccaacaagct gaagctggac taaatgatga agaagtcgaa cgattgcagc agctaagaaa
    11461 gattaaaact ccgattgaaa aaaatggtaa aaagctagaa atcaagcgtg aggttatgca
    11521 agatgtgcag attagcagaa aaaaacatcg caaaattgat gatattcttt caattgaaga
    11581 ttcattgact cggattagca accagttagc taaagccatc aagcaaatga atgaacttta
    11641 tatgaatgaa tacagaactg atttaattaa agctcagact gataagatcc aagctgagac
    11701 aaatgaaatt ggcggaaata attcaggtga agaaatagaa gaatggaaac aggcagtttt
    11761 aaatgccgca aacaaacggg cggtgaaaga aaatgaataa tgaatttatt ccttttgctg
    11821 atattggttc tgccattgat tattactatg ataaaccagt agctttttgc caagatattt
    11881 tgcatttgaa tcctgatgaa tggcaagaaa atgttttaaa tgatttagct gaattttcaa
    11941 aggtttctgt tcgttctggt caaggagttg gaaaaacagc attagaagca ggagcaatac
    12001 tttggttctt aacgtgtcga ccctacgcta aagtaatagc aacagctccg acaatgaagc
    12061 aactttacga tgtactttgg gcagaggtag ctaaatggtt aaatgatagc ttgatcaaaa
    12121 acttactgaa gtggacaaag accaaaattt atatggttgg tgattcagag cgttggtttg
    12181 ctacggctag aacagcgact aaaccagaaa gtatgcaagg ttttcacgag gaccatatgt
    12241 tgattgtggt agatgaagct tctggtgtgt ctgatccaat tatggaagct attcttggta
    12301 cgctatcagg ttttgataat aagctgttga tgtgtggaaa ccccaataat attgaaggtg
    12361 tcttttatga ttcccataat tcagaccgtg ataaatacag agttcataaa gtatcaagct
    12421 atgatagtaa acgtacaaac aaagacaata tagaaatgat tcttaaaaaa tatggaaaag
    12481 aaagtgatgt tgctcgtgtc cgtatttttg gagaatttcc gaaaggtgcg ttggattcat
    12541 ttatcagtct tgaaacagtt gaattggcta cagaaaaaca aattagtgat tctttagtca
    12601 ataaaacaaa ggttgctcat attggtgtcg acgtagctcg atatggtgat gattctacga
    12661 ttctctttcc tagaattgct accagggcat tggagtatga gaagtattca aaacgtagca
    12721 ccatggaaac aacaggatat gtcatcaaca tggccaagaa tctaatgagt caatatccga
    12781 gtattgataa agtgatgatt aaagtcgatg acactggtgt cggaggtggt gtaaccgacc
    12841 gcctagaaga acttatagaa gacaaacatt atccttttga ggtgtttgga gtgaataacg
    12901 gttcaacatc agaagacgat ttttacgata atttaggtac tcaactatgg ggaaacatca
    12961 aggaaatgtt agaagaaaat atgacagcaa atcttaacgg agaacagcct gttattgaat
    13021 tgccttctga tagttcgtta atcaaagaat taagtactcg caaattcaaa atgacaagta
    13081 gaagtcgtat acgtttagaa agtaaagatg atatgaaaaa gcgaaatatt ggtagtcccg
    13141 atattgctga cgcactggct ttagcgtttt atgagccacc aagtcactat caatttattc
    13201 aattttagga ggtgagcttt tgattagtac agtattatct ttagagagat ataagaagct
    13261 acgtgttaaa tatgcaacac aaattgaaga tggaatgttt gatccgaatg gttttataga
    13321 agatatgaaa ccattttttg ctgatcgcga aagaaaatat ctagcttata ctagcgaaaa
    13381 gaatgaaata gataatagac caaagcctaa cactgatatt gtaaaagtta ataataaact
    13441 tcatgctggt atgtattcaa ttgtcgtaga ccaagcagtc aatcatttta ctggtatacc
    13501 tatcaaatgg gattacgatg tatcggaaca aaaaagaaca ctcattcaaa gattaaaaga
    13561 taaattctta aaaaatgata tagaacttcc gacagttcca gaagctttta acaaattaac
    13621 aagcaatctt gattccatga gattcgcaat gcttgattct gaaactgcaa cttttcaagg
    13681 agcttgtgga gtagctttcc gattgctaga gcctgtggaa gaagatgatg gttggaagtt
    13741 aagagcaagt aatattgaac cttggagagc agaaagatat ggaaatgccg gaatctatat
    13801 caaagaaaag tatgactctt accagaaaaa attttttcaa gaaatgaaag ttatgacaag
    13861 aaataaaatt ctgacatatg cttgctatgg tgatttgaat tttgttacaa gcggaacgtt
    13921 caaaaaaatt gatgagaccg ataacccttt ggggacgatt accttggcag aatttaaaaa
    13981 taatacgaat cgttattgtg attttgaagt agctgaggaa attggagatg caattgatcg
    14041 tgctttgtct gatcaacaaa acgaaatcga acaatttaag cttgcgtaca tgcttgttac
    14101 tggcacaaca atgagcaaag gcactgctaa agaaatgatg aatcagctag ggattatcaa
    14161 cttaaaagcc cctactgcaa aagctgaata tgtcacaaaa aatttagcaa aagatttcaa
    14221 tgaatatcat atggatctat tgaaaaaaca gttttacacc atttgtaaag caatcgattt
    14281 taacgatgag gtgtttaaat caaacagttc tggagaagct cgcaagtggc aaatcattag
    14341 cctagaagcg aaaacaaata ctaaagagca atattttaga gaaggattga aagaatgcgc
    14401 agaaacaatt gctgcttttc ttaagtttca tgacaaagta gaaattgagc ctgaaaaaat
    14461 tatttttact ttctctagat ctttgccaac tgatttaagt tatttagctg aagctttacc
    14521 taaacttgca ccatacgtat ccaaacgaac tattcagagc caaattccat ttgtaacaga
    14581 tgttgattat gaaaatgaaa tgatggaatt ggaaagtgga agtgcttatc cagatagtga
    14641 atacaatttt ggcggaggtg gcaatagtga cgatagaaac gaagtattgg accaaacgtc
    14701 gagaactgga ggatcaagca aggctcaaac aagaaaatca gacacttaaa aaattaacta
    14761 gtgtatttcc tgaagcactg aaagagatac aagcaaaact attatcacaa gctgacttac
    14821 acaatatcac ttatccagaa atgatggagt tttacagtac aagtaatcag aaaaaatatc
    14881 gtgaatatgt ggaaaaaaat tataagtcat taaaaatgta tgatgcaaaa tacaaagagt
    14941 ttatcgatga attttttcca ccatttgact atgcaaaagt caatcgctta ttacaaatac
    15001 gatcagatgt atttaaaatt cttgcagaat atgcgatgga tgcagatgtg aatcaatatt
    15061 tttctgatcg cttagaggaa attcttcaaa gaacatattc ttctaatgct aatgtttttg
    15121 ttcaactttt aaacgttgat ataccaaatt atttaccaga aaatgaaatt aaacattatt
    15181 tgaactatcc atggtcagga aagacatttt caagaagact ttggggaaat atttcatcgc
    15241 ttgaacagaa actctctaat gctattgtaa aaagtgttgc tagtggagaa ggcgttatac
    15301 acgcattaaa caccatgaga ttagactcag aaatttgtga catgtttaag ttagaagaat
    15361 caaagtataa caaagcgata gaaaatctcg ttcgaacgga gtatgcaaaa tttgcacaag
    15421 atggtattga aaaatcatat ttagaaacag gtattgagga atacaacgta ttgactgcaa
    15481 aagatgagag agtttgccgg atttgtggag gaaaggcaag taagaatccc tataaactga
    15541 aagatgctgt cataggtgaa aatcgagcac ctttccatag tcgttgtaga tgtacggatg
    15601 ttcctaattt accaaaatta ggaaaggata ttgatgaaga atatgaccgt ttatttggcg
    15661 atttattaga tgagtttgca catgattctt ttggaattaa tttgaaacgg aggaagtaga
    15721 atgaaagatt tttttgaagc agtactaaca attaatgtaa atgctgatat tgcagaagcc
    15781 tacaaaacag ctattgagtc tgagaaccat cctaatggct tgagagacca ttggaatggc
    15841 aattatgcct acgtggttat tggcgatcaa actgttaatt atcaagataa tactccagtt
    15901 gataagaata ccgttaattt aacgattcaa ttattatctc attcattacc aaatttaaaa
    15961 gaaacagttg attggtatga aaagatggga tgtattgttg ttagaactga ctacaaagaa
    16021 ggaaagtcta gtaattaggc ttttttattt tgtccgaaat gacgttaaac tagcgcaata
    16081 ctgggcttgg ttgaatggtg gggcgcaact attaaaactc aaagcaatgc ggggcgtgaa
    16141 aacgaatcgt ggggcgaaag gagaatgagc atgaaacaca aatcattaat gccaatgaat
    16201 ttgcaatact ttgccgaagg tgacgatcaa aagttttctt ttgatgactt caaatctttt
    16261 gtggaatcca atgaagaagc tcaaaaattt attcagtcac aatctcaaag tgttgccgac
    16321 aaacaattgg aagcttggaa acaaaataac ttagataaaa ttaaacagga taccatcaag
    16381 gaatatgagg aatctaagaa aaacaagtca cctgagcaaa ttcaactgga aaaactacaa
    16441 gctgaatttg aagcagaaaa agcgttgcgt gtgacaagtg ataataaagc atttgttgca
    16501 gaacaaattg ctggattaga actagatgga gagttaaaag agtctatttc tcaatttatg
    16561 ctaaataatc ttgttagttc ggatacagat ttcactaaga atgctgttga aggttttaca
    16621 agtgttttga atgcaattaa tgagaaacat gcagatgcat taaaagaact acaaatgaag
    16681 tctgcatttg gtggaactca acaatcgaat aaccaggttc agcagaacaa tgaaacatta
    16741 acaaatccag aagaacaatt aggacaaatt cttcaacaat ttaactagga gagtgaaaaa
    16801 ttatgaaaaa aacatcttta aataatttag agtatttgga tatttcacca gcgattaatg
    16861 ctatgcaagt accaaataca ccttttttaa gctatttact tggtgctgga aaaacagagc
    16921 cagcaaactc gacagaaatt aaatggcgtg aatatgatat caacaacgat gattcttctg
    16981 aaaaacttga gggcggagaa tatccagatg ctgaatcagg tcgaacttgg tttaacaact
    17041 atactgaaat ttttagaaaa tcaacctctg tatctggtac attagatgct attaatgtga
    17101 atggagtcgg aaatgaatta actaatcaag tagcactacg tggtatggaa atgaaaattg
    17161 atttgaaccg aaaattgatt actggtgtaa aagctgatga aaatggttct aaaggtcgtc
    17221 gaatgaatgg gattttgaac ttgatcaatt cagcaaataa ggcagaaaca gccactgcgg
    17281 gtgcagtaac aagaaaagat atcgatactt tatttaaatt gatgtatgaa aaaggttata
    17341 tgggagaaaa actatgcttg atttctccag atatgcagga gttaatgact gatgagttag
    17401 atggaaaatc aacaaaaatt gttcagttcg gggaaagagt aacttttgga ttgcaaattg
    17461 gaaatatcgt gtctaattac ggtacaggta ttgctctact agaaccatca ttgccaaaag
    17521 gaacaattgc cgcaatcgat actaattatg tgaaactacg tccattacgt gaatggagag
    17581 cagaagaact tgcaaaaaca acggattcta gacgtatcgg tcttgtaggt gaatactctc
    17641 ttgaatacaa tgcttcaaac tctggggcaa ttttaaattt aaagtctgaa taaaagggag
    17701 ttagtactcc cttttttgat aggaggaatt acagtggtaa aaaaagatga aactaaaaaa
    17761 gatgaagtcg tgaaatatag agtaggtaaa actaaaaatt ttgttgggtt tgttcatcct
    17821 aaaactcgta gatttatcac agcagattca aataacgaat ttatcatttc tatagatgat
    17881 aaagaagcaa ttgcaatttt ggaagatgca attgatgtta atgaaattta ggaagtgatc
    17941 tgatggatga atcgctaaaa acggaaatca ttgagtctac aaaagaagat tttccagatt
    18001 tgagtgaaga acgcataact aatttattag aaataatttt gctagaaatt gaatcataca
    18061 acacttgtaa aaatgatatt tcatgggaaa agttaaaaag cgtgattagt gaagtgttgt
    18121 ataaaataat aaaaaatgaa tcagaaaaaa cagtatcttc agttagacgt ggcgatacaa
    18181 cgattagtta tgcttcaaca acaaatgatg ttagtgagtt acttctaggc tatggtgatt
    18241 tgatacgaag agttattggt tgtggaggat tggagttttt ttaatgaatg aagcagatat
    18301 tttagaaact acttatgaag atagttgtat tattgaaaga ctgacggaca ttgaagattc
    18361 taatacaaat attactattc aagattacaa aaaagtatat gataatccta tttcttgcgc
    18421 tctttcacaa ggtcaaatcg atggactagc agtcatagaa gatggagaga tggtaaatgt
    18481 ttcaactgac acatataaat tatttgttca tcctaagatt aaactaaaaa aaggagatcg
    18541 aataacaata actcaaaagg ccagtggctt aattttttct ctatttgcta ctaagccttt
    18601 ttactatcct agtcattgtg aagtgaattt gataggaagt gagaaaaatg ggtaatctca
    18661 aatatgaatc aaatgcagaa aagatcattg agaattttaa aaatatgact gtaattgctc
    18721 aaaaagaagg aatatccttc gttaacgatt caatgaataa agtcgttagt ttagctaaac
    18781 cattaactcc tgtaaaatca ggtaatttaa ggcgaggata tcgtgtagta aaagctagaa
    18841 aactatcaag cggtcgaatt gttggagaag ttataaacaa tgaacattat tttaaatatg
    18901 tagaagaagg tcataggact aaaaatggtg gctttgtcaa agggaaattc atgttaactc
    18961 gtgcaacgaa tcttgcaaat atgtcttata ttcctcgaag atttaaacaa atggcaataa
    19021 aaatcgttaa gaaaggaaag taacatgaaa gatgaaatca ttgctgcaat cagtagcaaa
    19081 ttaaaagaaa tctatcctga tgggacaata tatcttgatt cagttatgca gtcaactaaa
    19141 gatttttatt tcgttttatc agtaatggaa tctggaactg aaaatgtagg aattgatatt
    19201 caaaatgttt ctttcttaat tgatattgca ttgattgata ataaccctaa tagaaattta
    19261 attaatgaat tagtctcacg ttgtgggact ttttttaata cgattacaat cgatgagcaa
    19321 atattatttc caaaagccta tttacctgat gaagcagatg gtgttcaaca tattcgtttt
    19381 acattagaat ttccacaata tattgaatgg agtgaaagat aaatgggaga aaaaagaagt
    19441 aaaaccggaa ttatttctgt cgaaaaacct acttggtttc ctttggaaga tgagacaggg
    19501 aattttccgg tatatggtac tgcgttgcca atgggaacag cagtaagtat caaaccaaca
    19561 gctaattatg aaacaacaca agattatggg gattcagtgg ttcaagatca atttacagct
    19621 tttggtggtg cagaagttga actggaagca aatggatata ctcataaagt tttatctaca
    19681 attactggag ggaaaattgt taaaggtggt gccttgcgtt ctggagaaga tattgcgcaa
    19741 gatggtgcgt ttgcatatag acgtaaaaaa tcgaatggga aataccgtta tacggtcttt
    19801 tataaaggac agtttgctct agattcagat gaaacatcaa cgattgaagg aagtaaagta
    19861 agttttactc atccaacttg gaagggttca tttgtagatg taccaggact tggatatatg
    19921 tattcagttg atgaagatga tgaaggcgtg gataaagcaa tgatcggaaa ttggtttaca
    19981 aaagtagcaa ttccaattga agaagcagaa ctttcaggag gtacagaata atgtcaaaat
    20041 atcaaacaac gattaaatta acgaaaaaag acgaagaagg taaatatgag caagtacaat
    20101 ttaaatctgc tgaattttta ccaggaacag ttgtagaaga tgctgcagga gttatggaag
    20161 aaatgcaaac agcgactgat aagcaatcag ttaaaaaagc tttaagtcgt gcttattcat
    20221 ttattgcaga tactttattt gagggacaat ttactggtga agattattgc aaaggaattg
    20281 atgctcgtga gattgcctca ttgacaggaa aattattgaa gtctgttact gcaggttttg
    20341 atgaaactta tacagagacg aaaaaaaagt aagtgaggcg ctcaagtcac cttcatttaa
    20401 gtattcgatt acttaccgag aattagatat aaagacgcaa ttactcgaag caggttggac
    20461 gttaccagaa attgaacgta ctgacttgga tgagttaatg cgtctttttg cttttagaga
    20521 tgcggtaaaa gagcatgaag atgttgagta ctatgataat ttcactcaat tttaggaggt
    20581 gatactttga ataacgatga cctaattctg aagatgatac tagatgaatc tggttttact
    20641 gccggtatga ataatgctgt caaaaagttg aactcttttg atgaaacaat cgaaagaaaa
    20701 agtagaaata gtggcagttc attaggtagt atttggaaaa tatttgctgg aagttttcta
    20761 gctagcggag taactagaat cgtaggagct ggctttgatc taatcaaggg ttccataagt
    20821 ggagcggttg atcgagtaga tacgatgaat aacgctctac gaaatttcca aaatatggga
    20881 tttagtaatt cagaaattat gaagaatatc ggaaagaatg ggcttttatc tcaaggtatt
    20941 caaggacttc ctactgcttt gaatgatgcg ataagtcatg ttcaacttct tgcttcttct
    21001 acaggtgatt taactcgttc aactcaaata tttaaagctt taaatgatgg aattcttggt
    21061 ttcggtggtt caactgatca agttaatgaa gctgttattc aattatctca aagtttctca
    21121 aacggaaaag tagacgcaca aacttggaac tcaatgatca acgctcaact tggacctact
    21181 ctttctgcta ttgctaaaaa gatgggaatt acaatgggag atctgaaaga aggtttatct
    21241 caaggtaaga tttctgttga agaattccaa aatcaattaa tagaaatgga taccaaaggt
    21301 ggcggaggac ttaaatcatt aagtcaaatc gctaaagatt ctactaaggg aattaaaacc
    21361 tctatacaaa atgcaaaaac agctgttaca cgtggtgttg gtgaggtaat agaaggatta
    21421 aataaagcat tagtagattc agatttaggt ggatttaagg gaattattga taaagtgggc
    21481 agttcaatgg aatcgttctt aaaagtaatt gctgcaaata ttcctacagc agtatcattt
    21541 ttaagtaatt tatttaacga aattcaaaaa tttggttctg ctttgaaatt catgatgccc
    21601 tttcttgttc ctgcagctac cgcttttgga gcgttcatgt ttcaacttaa aggtatacca
    21661 gcaataataa aaagcttcaa taattttaag aatgccataa ttggcgttgg aaattcatta
    21721 aagattatgg gggcaatagc tgctgctaat ccatttgttt taattattgg tgcagtcgtt
    21781 ggtgcaatag ctgttttcgg ttactttatg gcaaccaatg aagagcttag aaacaaggtt
    21841 atatctattt ggaatgatgt gaaagattcc gtaattggtg tattaaaaaa tataaaggac
    21901 tggggaattg atacttggaa ttctgctaaa gaaatggcat caaatgcagt tgagggtgtc
    21961 aaagatgctt ggtcaggaac aaaggaatgg ttttctaaca cctggaaagg tattaaggat
    22021 ggggccacag gtttatttga taaaacagta gagacttcaa gaaatgcagt cgatagtgta
    22081 aaaaatgcat ggtctaatat gaagaaatgg ttttctgata cttggcaaag tataaaagat
    22141 tcagccagag aaaaatggaa tggaatcaaa ggttctatta tgtcagttgc tggtccattg
    22201 attacaggta taaagaatgc atttttacat gttacttttt atctagagac cttatggaat
    22261 aatttagttg agattggtaa aaacgtcttt gagattttaa aaaatgttat acttgcgccg
    22321 gttttgttta ttacctccat gatttctggt ggatgggaag aaacaaaaaa taatatgatt
    22381 gctgtttgga ataatattaa agaaagtgcc ataaatattt gggaatctat aaagaatata
//
LOCUS       DAZKOB010000040        22336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000040 DAZKOB010000000
VERSION     DAZKOB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.040"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG8M6_001467"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ACG8M6_001467"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0457144.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG8M6_001468"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ACG8M6_001468"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0457145.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG8M6_001469"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ACG8M6_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0457146.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG8M6_001470"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ACG8M6_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0457147.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG8M6_001471"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ACG8M6_001471"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0457148.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG8M6_001472"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ACG8M6_001472"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0457149.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ACG8M6_001473"
     CDS             complement(8557..9027)
                     /locus_tag="ACG8M6_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0457150.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ACG8M6_001474"
     CDS             complement(9073..9498)
                     /locus_tag="ACG8M6_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0457151.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ACG8M6_001475"
     CDS             complement(9565..9834)
                     /locus_tag="ACG8M6_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0457152.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ACG8M6_001476"
     CDS             complement(9845..11440)
                     /locus_tag="ACG8M6_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0457153.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG8M6_001477"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ACG8M6_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0457154.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG8M6_001478"
     CDS             complement(14993..15553)
                     /gene="pth"
                     /locus_tag="ACG8M6_001478"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0457155.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="ACG8M6_001479"
     CDS             15906..16880
                     /locus_tag="ACG8M6_001479"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0457156.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="ACG8M6_001480"
     CDS             17050..18450
                     /locus_tag="ACG8M6_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0457157.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="ACG8M6_001481"
     CDS             complement(18528..19250)
                     /locus_tag="ACG8M6_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0457158.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="ACG8M6_001482"
     CDS             complement(19329..19835)
                     /locus_tag="ACG8M6_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0457159.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="ACG8M6_001483"
     CDS             19958..21757
                     /locus_tag="ACG8M6_001483"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0457160.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21825..>22336)
                     /locus_tag="ACG8M6_001484"
     CDS             complement(21825..>22336)
                     /locus_tag="ACG8M6_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0457161.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6176 a         4872 c         4033 g         7255 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ctgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttgattaatc attcttacat gctgacagca
    21841 aagtcagggg caaaccattg aactgaacca acttttacgc tttcgcctgg ttgaggagca
    21901 tggatatatt gtcctccgcc taaagcaatc gctacgtggt aagttccgcc ttgtgacccc
    21961 cagaataaca agtctccagc ttttgcttga gaaactgaaa tttttgttcc agctgattct
    22021 tgaggaactg tccaaccacc gatgtcacga ccagttactt gcatgtaaac atagcgtgtg
    22081 aaccctgagc agtcaaagcc acttggatct ttaccgcccc aaacatatgg agtaccaata
    22141 tatttgtaag cttctgctac gattgctgca ccattcacac ttccagaagg agctggagca
    22201 ggtgttacag gttttggttg ttcaggtgtt ggtgttggcg ttggagccgg agtactactt
    22261 cctgtgccgt ttcctgtatc aacactttga tctgttgatg gtgttgtagg cgcaggtgtt
    22321 gttgattctt ctgttg
//
LOCUS       DAZKOB010000041        22160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000041 DAZKOB010000000
VERSION     DAZKOB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.041"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="ACG8M6_001485"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="ACG8M6_001485"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0457162.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="ACG8M6_001486"
     CDS             2370..3848
                     /locus_tag="ACG8M6_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0457163.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="ACG8M6_001487"
     CDS             3814..5145
                     /locus_tag="ACG8M6_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0457164.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="ACG8M6_001488"
     CDS             5259..7811
                     /locus_tag="ACG8M6_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0457165.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="ACG8M6_001489"
     CDS             complement(7818..9257)
                     /locus_tag="ACG8M6_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457166.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9232..9414
                     /locus_tag="ACG8M6_001490"
     CDS             9232..9414
                     /locus_tag="ACG8M6_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457167.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9603..9965
                     /locus_tag="ACG8M6_001491"
     CDS             9603..9965
                     /locus_tag="ACG8M6_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0457168.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="ACG8M6_001492"
     CDS             complement(10152..11009)
                     /locus_tag="ACG8M6_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0457169.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="ACG8M6_001493"
     CDS             11129..12040
                     /locus_tag="ACG8M6_001493"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0457170.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13693
                     /gene="mdcA"
                     /locus_tag="ACG8M6_001494"
     CDS             12047..13693
                     /gene="mdcA"
                     /locus_tag="ACG8M6_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0457171.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13795..14094
                     /locus_tag="ACG8M6_001495"
     CDS             13795..14094
                     /locus_tag="ACG8M6_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0457172.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14084..15760
                     /gene="mdcD"
                     /locus_tag="ACG8M6_001496"
     CDS             14084..15760
                     /gene="mdcD"
                     /locus_tag="ACG8M6_001496"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0457173.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15764..16357
                     /locus_tag="ACG8M6_001497"
     CDS             15764..16357
                     /locus_tag="ACG8M6_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0457174.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16381..17457
                     /locus_tag="ACG8M6_001498"
     CDS             16381..17457
                     /locus_tag="ACG8M6_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0457175.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17482..18351
                     /gene="mdcB"
                     /locus_tag="ACG8M6_001499"
     CDS             17482..18351
                     /gene="mdcB"
                     /locus_tag="ACG8M6_001499"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0457176.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18462..19226
                     /locus_tag="ACG8M6_001500"
     CDS             18462..19226
                     /locus_tag="ACG8M6_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0457177.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19715..21808
                     /locus_tag="ACG8M6_001501"
     CDS             19715..21808
                     /locus_tag="ACG8M6_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0457178.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21833..22135
                     /locus_tag="ACG8M6_001502"
     CDS             21833..22135
                     /locus_tag="ACG8M6_001502"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0457179.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7177 a         3786 c         4721 g         6476 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaccagt
    13381 gatgatttat agcgaagata ctacacatat cgtgacagaa gaaggaattg cttatttgta
    13441 caaagcaaaa acaaaagaag aacgacaagc agcaattgca gcaattggcg gtgttacacc
    13501 tcttggaatg actagcacga aagaatcact agatagcttg cgtgcagccg gcatcgtgaa
    13561 attaccagaa gatctaggca tcaaacgtag tgaagcgaaa cgttcattat tagctgcaca
    13621 aaccatcgat gatttgatcg aatggtcaga tggcctgtat gaaccaccaa tgaaattcag
    13681 aacttggtca taaaattggc gagaaaaacc ttttgtcgcc atttacccag atggaagaag
    13741 aaagacgaat aagcagaacg tgtatgctct gttttacaaa aaggggagag atccatggaa
    13801 caattagctt ataaatatcc aacgaaacag ccgatcaata agaaaatcca tgtaggagtg
    13861 gtcggttcag gagatttgga aatcttgatg tttccaacag aaaaaacaga atctactgta
    13921 aaaatttgta caggaagcga tggcttcggc gaagtatgga acaatgtatt aacgagattt
    13981 tttgatcgtt atccaatttc agcagatatc gtgatcaatg acttcggtgc tacaccagga
    14041 gttgtgtacc tacgattgac gcaagcaatg gaggaacttt cagatgaaaa atagttttgt
    14101 agaattaaac gccagagaac gagtgaacgc tctacttgac aatggacgtg aattgattga
    14161 tccattcgat caaatcatcg cgccaagttt accagcacaa ggaatcgtcc ctgaaagtga
    14221 tgacgggatc gttgtcagcc gtggcacgat caatggcaaa caagcagtag taatcgctat
    14281 ggaaggaaaa ttccaaggtg gcggtatcgg tgaagtttcc ggtgcaaaaa tcgttgctgc
    14341 acttgaacat gtgttagaag aaaataaaaa aggaaatgag atctttccag tcattgtact
    14401 agatacaggt ggtgttcgtt tgcaagaagc aaactacgga ctgttatcta tttcagaaat
    14461 cggaaatgta atcattgctc taaaacaata tgtaccagtc atcggtttga ttccaggacg
    14521 tgtaggatca tttggcggta tgtcgattac ttcagcatta atgagttatt taatcgcaac
    14581 aaaaaaagcg agagtcggtt tgaatggacc agaagttatt gaacaagaag caggcgttcg
    14641 agaatttgat tctagtgaca aagacttgat ttggaataca ttaggatctc gtcaacgggt
    14701 agaatcaaaa atcattgatg aattagtgac tgatagtgta gaagcaatca aagctgctgt
    14761 catcgcagca atggacgaac gaaaagacac gcatcgcagt gaaagctcag cattttattt
    14821 atcattactt gattctttag atctttcaaa accaatggcc attgacgatt acaatgaagc
    14881 aattgctaac catcaagcaa aatcaattga cttaccagaa gtagcagaag gaaatgaacc
    14941 agctgcagca agtgttggtt acgattggtt tgcggcacta actggcatga aaaatccaac
    15001 ttcttctatt agtactgtgt attatggtaa atcaagtaaa ttcatagaag aagccatcgt
    15061 gacatcaatc gtacctgatc ctaaaaaccc gatgtaccgt gtacgaaacg gtgaagttgg
    15121 tttagttgaa ggtttccgca tgggacaaat tttggatcac gtttacgaag aagataaaga
    15181 caaaaaagtg aaacggccaa tcatcgttgt gatcgatgta ccaagccaag catacggcta
    15241 taacgaagaa ttgatcggta tccatgtggc tttggctaat agtgctgctg cttatgcaaa
    15301 actccgtcaa gctggacatc cagttattgg attgattgca ggaaatgcta tttcaggtgc
    15361 atttttagca catggattac aatcaagccg cttgattgct ttaaacaata aagaaattac
    15421 cgtccaagcg atgtctaaag aaagtgctgc acgtgtgaca aaacgttcgg ttgctgaaat
    15481 cgaagcagct gctgaaaaag ttccagctat cgcttacgat atcaataatt atacaaaact
    15541 aggagcgatc tatcatttcc tagaagatat caccgaccaa gctgcttcag aacaaaatgt
    15601 cagcaaagtc atcgaagcaa tcaatgaagc cattcaagat gtccgcaata caaatgacac
    15661 gatgctgaca aatcgataca caaacgaaac agccaaaaca ttcggacgtg tcgaaacaaa
    15721 taaagtccgt gccaaaatgg acgaagaatg ggatgcataa aaaatgattc aagcacatga
    15781 tattgtcctt tttgataaac atgaaataga agggttcacc ttgcctgaat gggtcgttct
    15841 aactgaaaag atgttcggaa cagttcgtcg tgcgtcgacg gatcacgcag aaatactacc
    15901 gattggttta cgtggacctg cccgcaatca gagatggtca acagaaatct caaaacatgc
    15961 cgtcaaacaa gtcattcatc cgtgggacat catcgaacaa gaatcgtata gacaagagaa
    16021 aatcatggat tatcctgttt atcagcaatt tcatgctgct agaaacatat tatcaggatg
    16081 taaatgggga atcggcggca gcttaggatt tgaattatcg actgggattc cggcagtaaa
    16141 agaaaccagt gattttgatt tgctgctgta cgcaaattca ccgattgaat tgccaattca
    16201 ggtaattcaa tcgcatcctg ctttttttga gcaattcgat acccaagtag tgactcggca
    16261 aggcggtttt tcactaaaag agtatttgag agagccagag aaaaaaattc tattgaaaac
    16321 agtcgaagga cctaaattaa ccaaagaact ttggtaataa agaaaaagag agagaaagag
    16381 atgtttgaaa acaaaaaaat caaaatcaac gtaagtgact tccagaaagt atcagctgta
    16441 acggctgtta tgatgcaaac gatattgagc tttgctttgg caaacagcca cagcgcaaca
    16501 aatacagctt ttcttggtac cttttacgtc atcgttaaat acacagcacc aatgtttatt
    16561 tttgcaattg tttataatat ggtaaaaaca agtcagcatt tgtcttattg ggaatttttg
    16621 aaagaaaagt tttttgaact agtcgttccg tatcttctat ggacgacggc ttatctttgg
    16681 ctatttccag cagtccaaca acaaactccg tatacggata ctgcaagctt tttactgaaa
    16741 tatgtcacag gtgatggagc gcctcattta tggtataccg ttatgatgct gcagatccaa
    16801 ttgttcatgc cgttttttgt ttggctcggt tacaaagtat ttgctaataa aaaatatgta
    16861 tggccagtat tgattggttc aacgatttta tatgtcgcat ggtatctgtt ctatcaaaca
    16921 caagtattga ctgggcctta tcatgacagc tggtacttat tagatcggtt tgttgctagt
    16981 ttcatgattt atggggtagc cgcatttgtt tatcacgaaa aagtctatca atatttggac
    17041 agagtacgtt atcttttctt acctgttgcc ttagttatcg cgttcttttc tgtccgttca
    17101 ttgctcgcac atccaggaga cttgagcttt gcgaatgcac cttacttgaa tacgatccaa
    17161 agtttataca gtttagtcat catctttgct gtcttcatag gcgcttccaa aatgattgta
    17221 aacgattcgc caaaattgcc attgttcaaa tggttgtctg tttatgctta tcgtacgtat
    17281 ttagccaatg tatttgtttt ccaagtactg ttattattgt ttaaagatag ttggctgcag
    17341 ttaccttcag gagtcatgat tcttgtagct tatctaatga cggcaagttg tgcatttgct
    17401 ttaagctggt tacttcatat catttgggta gctatcaaaa aaggtttcag caagtaatag
    17461 taggaagaag gagtgagtgt catgataaat gacgaacgaa aaaaagcagt gactcagcaa
    17521 ctcgctgcgt tggtagagga agcattgatc gcagaagtaa cattaagtcc aaaacctgga
    17581 ttagtggatg cactggatgc gggtgcacat tccgatatgg actatgcttt atttcttaag
    17641 tctgctaaga cgctaactcc tttttttgaa gaaatggctc aaatagcatg gcatcatgct
    17701 attgatcagg agttaagaga aagaattgct gagattgggc gaaaagcgga aaaggcaatg
    17761 ctgattgcta caaatggcat caatacccat aaaggagcga tttgggcaat ggggctattg
    17821 atcagtgtca ttgcacaaaa aatcagtatg gaacagcctt tttctttatc gaaaatactt
    17881 gatgatgtca gcaagcttgc tgcttttcct gatacaaaat atcgtcataa aaaaaccact
    17941 catggtcata gggtgaagca aaaatatggc gtaaatggtg catatgaaga agcgatatta
    18001 gggtatccgc atatcaggct tgcaattcaa gcagctaagg agctagataa tgattcatat
    18061 gtacagaaac aaatgcatat gttgttagtt ttgatgagtt cgctagatga cacatgcgta
    18121 ttgcatcgca gtgatcagaa aacgttagtg gaaatgcaaa aacttgctca tgctgctagt
    18181 caaagtgctt taccaaatcg tccatttaca gcactgttgc acttttgtca ggaaaaccaa
    18241 atctctcctg gaggaagcgc agatctcttg tctgcaagct tgttcatgct gaagatagag
    18301 gaattatgga ctgccgacac atacatttgt ccgatgttac tcaatgacta aataagacaa
    18361 aattgctaaa aaaacagttt atgcatctgg ttattgactc ttccttttag caaggctatc
    18421 ataaaaatag ttttttgtaa taaaagataa cgaggaagac aatgaaaaaa atcaattatt
    18481 tagcaatcat tttgttagtg ggcctatgtt tgtttgctct gccgacccaa ggattcgctg
    18541 tcgcgggtgg tcatggtgga ggtacatcta gcggtcgaag ttcgggcaga ggttcatttg
    18601 gtggtcgttc atatgggggc agttattttg gcagtcgttc ttacggcaga gattcttatg
    18661 ggtattatgg tccaggtaca ggtccttcta gttatgagca aacgggattt cttgcattag
    18721 ctgttttgtc agtaggaggg gtttctgctt tttctatcgt caagaataaa agacaaaaga
    18781 aaaggctgaa agcggagata ttgtcccgca tgtcaggaac ggctaaagaa aagaaagcac
    18841 agtttctgga aatccaaaag atattcatga agatccaatc cgcttgggaa gaagaaactc
    18901 ctgaaaaagc cagaagctgt tatacagaca agctgtttgc cgaacatcag aaagttctgc
    18961 aaaaaaacaa agcagacggt gtaagaaatc atactaaaaa aataactatt caggaagtag
    19021 gaaattatcg acagatccac gaaaatagtt ttgccatccg aattgatttc agatgctgtg
    19081 attattcagt cgatcgaaaa gatgggcgat tgatctcagg aagtaagaca caacgacagt
    19141 atttttctca gatatggtat tttgattttt ctgagaaaga aaaaagatgg caagccgatt
    19201 ttattcagcc gattatgctg gattgacagg aaatatttat gaacggagtt gtgacaaaag
    19261 ccttgtcgca gctccttttt ctgaataaac ggtgtgccag agatagccta tcggcaataa
    19321 ggcacgattt tgtaaaatat gggaagcgat ttttcaaaat cgcttcttat cgctcaaggc
    19381 tgaacatctc tgtcacaacc tctccactaa atggtgttta aaagcttgca tctgcgataa
    19441 taagaacaac caaacgaaaa ataggaagca ctattttagt ttagttgttc ttattacttg
    19501 atgcaggaca gctttttcac aacctctttt ttgtatagat gggcaaaatt tttgtaataa
    19561 taggcgtatg tctttttcgt tgaggagatg aaaaagcata tggcttattt tttgtttgct
    19621 ttgcttactt tctttccact tcaagtggca gaaatatcta ctttttatta tgtaagcgga
    19681 accatatact tgaaatcaga aggagatcat ttctatgaaa acaagaacaa ttgaaatact
    19741 tcacagaatc atgacagccc aatatccagt aaaagcaaaa catctagcaa gtgagttcaa
    19801 tgtcagtcag cggacgatcc gtcaggaagt tcttgaagcg aatatctggt tgagcagtaa
    19861 aaagcttccg gagatccgta caattcgcaa caaaggtttt ttgttgaagc tggaaaagga
    19921 aaagcaagct cgattagaaa ctgaaataaa agatgtgaaa gacggctttt tgaatcggcg
    19981 cgaacgtatt tttgatctag ttttagccgt tgcttacagc cgaatcccga tccatctaaa
    20041 caaaaaagaa gaagaatttc aaatctctaa aagtacatta gatgaagata tgagacggtt
    20101 acgagcaaca ctggtcagtt atgggataga gatcgtcagt tatggaaagc aaggactggt
    20161 ttataaaggt tcagaacgat ctatccgtac gatgatctat gattttatca atcagaatct
    20221 gggaatgacc gacttcttta tgaatcaaga tgcacaatta acaccggctc aaaaaatctt
    20281 tctccattat tttcctttgg tggaaatcaa aaaaatacag gagatctatt ccaataagtt
    20341 gttgaaacaa gaagatgata tttataaaaa acaaatcctg ctttttacat tgatctggat
    20401 ccagcgaatc aggaaacatg aatttatctc tgccattaat tggaaaaata cagaaataaa
    20461 agacagtagc ttttctgcat ttattgacca catgatcgag gaatttcaat tggacaatgt
    20521 cccgcaagta gaaagaaatt atcttcgttt cactattgag acgttcaatg ctagagatat
    20581 ctccaattca atcgagtggg tacaagctca attactaacg atccaattga tccaatttgt
    20641 agagaacgag acacacgtac ctttccatct aaaggaagaa gctttattcg aaaatctcta
    20701 taagcacatg gctgcactga ttgtaagaat caagaatcat atccaagtca tgaatccatt
    20761 gaaagaaaac atccgaacaa attatttccc aatctaccgc gcagtcactc attttgttcc
    20821 tacgatcgaa gaagtaattg gtggcaagat actggaggac gagatagctt ttcttgtcat
    20881 ccacttttcg acgatcgcca gtgtaatcaa gcgagacttg aattatgttt ataaatcagt
    20941 cgttgtctgt aaccatggga tggctactgg aaatctgtta gcagagaatc tgaaagaaaa
    21001 gtttcctcag atcgagatta ctgcagtttt aagttcaaaa gaaatcgaat tagtagataa
    21061 attggatgtc aatctggttt tcagcacctt tcaattgaat tatcgaaata agccgctttt
    21121 agttgtcgat ccgattttaa ctgagacgaa tatcttggtt atagaagact ttttgaaaag
    21181 aaatgaagaa ctacagcggt tagagcctaa cagtcatgaa tcgacggagt tattcactca
    21241 aataatagat gtcatgaaag aaagtggtgg ccgagtagat cgaatcatct atggcaagtt
    21301 agaaaaatta ttcgaaacaa acaatttaga aatcaacaaa agggagatcc agcctatgtt
    21361 aaaagatatt ttgacggaca gcaatatcat gatcaaagaa aaagccacta cttgggagga
    21421 atcgattgaa ctagctgcta gaccattggt taaagaaaac attgtcgaag agcgctatgt
    21481 cgaagcgatg attcgtgcag tgaatgatta cggaccgtat atcgtgattg gcaaacacat
    21541 ggcactagcc catgcccgac cagaagatgg ggtcaataaa ttaggagtca gcgtagctac
    21601 gattgagcaa ccaatcgatt tcggtaatcc tgaaatggat ccagtaaaga ttattttttg
    21661 tttggcagca gtagactcct actcgcatct gaacatcatg aaagaactaa ttgaattgat
    21721 caacaacgaa gagaagttgg atcgattgat tgaatgcaca aatatcgaag atttcaaaca
    21781 actgcttttt tcagtagttg agaaataata aatgatacgg gggagaacaa agatgaacaa
    21841 attgaacatt ttattcgtat gcggtgcagg tttaggaagt agttttgcag cacagatgtc
    21901 tgctgaggat gttttgaaca aattaggagt agaagccaaa ctggaccatt gtgatatttc
    21961 ttccgcagtt tccatgaaac cagatgtgat catcacagca gaaaattttc gttcgcagtt
    22021 cgaaaagttc gcagttgatc ctaaaacggc gatgatctat ttgaaaaata ttgtttcaaa
    22081 aaatgaaatt gaagaaaaaa tcaccccagt tctgcaagat aagggagtta tctaaataca
    22141 aaaagaaaag agggtaaaaa
//
LOCUS       DAZKOB010000042        21814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000042 DAZKOB010000000
VERSION     DAZKOB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.042"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1432)
                     /locus_tag="ACG8M6_001503"
     CDS             complement(41..1432)
                     /locus_tag="ACG8M6_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457180.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            1689..5078
                     /locus_tag="ACG8M6_001504"
     CDS             1689..5078
                     /locus_tag="ACG8M6_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0457181.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            5081..6502
                     /locus_tag="ACG8M6_001505"
     CDS             5081..6502
                     /locus_tag="ACG8M6_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0457182.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6499..8376
                     /locus_tag="ACG8M6_001506"
     CDS             6499..8376
                     /locus_tag="ACG8M6_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0457183.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8468..9310
                     /locus_tag="ACG8M6_001507"
     CDS             8468..9310
                     /locus_tag="ACG8M6_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0457184.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9462..10337)
                     /locus_tag="ACG8M6_001508"
     CDS             complement(9462..10337)
                     /locus_tag="ACG8M6_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0457185.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10544..11574
                     /locus_tag="ACG8M6_001509"
                     /pseudo
     CDS             10544..11574
                     /locus_tag="ACG8M6_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            11663..11968
                     /locus_tag="ACG8M6_001510"
     CDS             11663..11968
                     /locus_tag="ACG8M6_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0457186.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            12147..14162
                     /locus_tag="ACG8M6_001511"
     CDS             12147..14162
                     /locus_tag="ACG8M6_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0457187.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            14164..14448
                     /locus_tag="ACG8M6_001512"
     CDS             14164..14448
                     /locus_tag="ACG8M6_001512"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0457188.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14474..15847
                     /locus_tag="ACG8M6_001513"
     CDS             14474..15847
                     /locus_tag="ACG8M6_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0457189.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15930..17945)
                     /locus_tag="ACG8M6_001514"
     CDS             complement(15930..17945)
                     /locus_tag="ACG8M6_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0457190.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17967..19181)
                     /locus_tag="ACG8M6_001515"
     CDS             complement(17967..19181)
                     /locus_tag="ACG8M6_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0457191.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19401..20345
                     /locus_tag="ACG8M6_001516"
     CDS             19401..20345
                     /locus_tag="ACG8M6_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0457192.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20641..21585
                     /locus_tag="ACG8M6_001517"
     CDS             20641..21585
                     /locus_tag="ACG8M6_001517"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0457193.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7412 a         3990 c         4117 g         6295 t
ORIGIN      
        1 ttttatcagg gtggtttata catagctgtt ttttgattat tcataactcg aataagcgga
       61 tgagttcttc ttcatttgcg aatgacacac tatcatcaat caatatatga ggaacatggg
      121 cattcaaaat gggttgattc gtaacgatca gatcatactc gagaagattg tatttcgaca
      181 gctgatcttc agtaatagca gttgtctcag agtgaatttt atgcacagca agaatatcac
      241 tgatatattc ttcaatatat tgactatgtc gaagacttaa gtcgctgata atcagtattt
      301 ttttatcaga agtataatgg tgaatttcag gattaccgat aagcatccaa aagatatagc
      361 tagataaata ataaatcaga ttctgattca gtagagtgga ataaaattga agttctgcac
      421 gaaatccagc atatagacgt atctggcggt gttgcatctg ctgtgcaaag taagttgcac
      481 ggatcgtcaa atgagtcgta tcgtatggga aaatacggga gaaaaaataa atttgcgtta
      541 ataattgaac aacatattct tcgttttttt ctttgcaatc agcggatgaa aacgaacagt
      601 aggacatatt ttttcgtatc tgctcactga tttcattttt ttctttctcc gaaaattctg
      661 aaaaaatcgt attgacaaaa tagttcacta cttttatata gctgctgtac ttcaattttc
      721 ctaagagatt tttcaagatt aagaagtctt ttttatcgat atttatcaac tgtacataag
      781 agtctccagc tttccattca aatggagttc cttgggattc tctcatgaga tgcacaacaa
      841 aatacataat aaagaaagat tgctcatatg ggctttcttc ctgaaacaaa ttcatctgac
      901 aattattctt gaccataatt tctttgatct tctttttttc gattggaaat ggcaacgtgc
      961 gtcttccgta gtaacttaaa aagaaaaagg taacaaagat acgcaactgg agctcacttt
     1021 taaggctagt taactgaaaa ccttggtcaa cgatcacttt taataaatag ctttctaaaa
     1081 tttgattgca ttgtttcaac ttacgtgtaa aagaagaccg gctgatggaa attttttcac
     1141 aataatactc aatcgtttta cccggattga caataatctc atttaataaa cgactgtttg
     1201 tagaactttg cgacatttcc atcagaacat ttaaataagc tggtacattc aaatgttcaa
     1261 gacgataccc caatttttgt gaagattgaa tcgttacatc cgttccccag cggattttga
     1321 tctcttcgat ataccttaca aatgtagatt ctgaactacc aattcgtttg gcaagttctt
     1381 ttttagtcat ccacttgtca gaaatggaca gaattcgaat gattttttcc atctttctat
     1441 agtttaattc caacaacctg atcatatgtt tttctccctc aacccttttc tttcaatgaa
     1501 acgctcatta atcgaaggaa tcaacttcat aaatgaaacc aattggtatg aaaattggtg
     1561 tgattccctt tagcgccctt cgtataataa ttatcatatt aaaataaata aaattatgta
     1621 acgcctgttt attttaataa ttttcactat tcacgtcaaa acagaaaaaa agaaaaagga
     1681 ggggaaaaat gacgaccaca gggaagaaac tgaaagttat tttcatgctg ataatattga
     1741 gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac tacagatgat ccaacagttt
     1801 tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg aaaaaaagca ctgaacatca
     1861 agctaaatgc gaataacacc agtgctgaaa agatagaaaa agaaattggt ctagtcgaaa
     1921 attacttaag tgatgtggaa agaaaagaag gagatggcta tgcttatcag gtaaatagcg
     1981 ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac tatcgatctg agttttccaa
     2041 tcgatccagc actttaccac agccaggcaa acaagctgat cgtcgataat aaagaatatg
     2101 acattattga tgagacagaa aataagaaag atacagatgt gtcagtacca aagccagacg
     2161 aaatagaaga agaatcatca aaagaaaacg aaaattctgt cagcccattt acattgccta
     2221 cattatcctt gccagctgtg agtgtgccat ctaatcaaac gattcctaca gaatatacaa
     2281 cagatgatca gggcacttat cctaaagcca gttggcaacc tacaggaaat acaaatgttc
     2341 ttgatcatca aggcaataaa aacggaacaa atcaatggga tggtataaat tcttggaatg
     2401 gagatcctaa tgatcggacc cattcgtata tcgaatatgg aggaaccggt aatcaagcag
     2461 actatgcgat acgaaagtat gcaaaggaaa caagtacacc cggattgttt gatgtttatt
     2521 tgaatgctcg tggaaatgta caaaaagata tcacgcctct tgatctcgta ttggtcgtag
     2581 actggtcagg aagtatgaac gacaataatc ggatcggtga agtaaagatt ggtgtcgatc
     2641 gttttgtcga tactttagca gatagcggta tcacagacaa aatcaatatg ggatatgtcg
     2701 gctactcaag cgaaggatat agctacagta acggtgcagt acagatgggt tcatttgatt
     2761 cagtgaaaaa tcaagtaaaa tccattacac cttcacggac aaatggtggt acttttacac
     2821 aaaaagcact aagagatgca ggaagcatgc tatccgttcc aaatggacat aaaaaagtga
     2881 tcgttttgct gacggatggt gtaccaacat tttcctataa agtacagcgg gtacacgcac
     2941 aatcaagcag caattattac ggaactcagt tttctaatac gcaagatcgg ccgggaaata
     3001 cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa caatctatcc agaagaatcg
     3061 acagtacgtt tatcgcaacc atcggagaag cgatggcact caaagaacga ggaatcgaaa
     3121 tacatggtct tggcatccaa cttcaaagcg atccggcagc tggtctctca aaagcagaag
     3181 tagagtctcg tatgcgacaa atggtttcat cagatgaaaa aggcgatctt tactatgaat
     3241 cagctgatca tgcaacagat atctctgaat acctagccaa aaaagctgta cagatctcag
     3301 caactgtaag caatggacaa ataaatgatc caatcgcaga accattcatt tatcagcctg
     3361 gtacactttc agtcaagagt gtggggacaa gtcctacaac ggtcactcca tctatttcca
     3421 tagaaggaaa taccatcaag agcaatcaga tctatttagg aaaagaccaa gaaatccaaa
     3481 tccattacca agtgagaatc caaacagaaa atgaggactt ccatccaaat ttctggtatc
     3541 aaatgaacgg caggacaact ttccagccaa acattgatac caatgaatta gctgaattcg
     3601 gtataccatc tgctaaagct cccggagtca gtcttcacat caaaaagtta tgggaagaat
     3661 ttgacaacaa tctagctgat cgtccagatc aagttacttt tgagattcaa cgggaacata
     3721 cgacaaatgc tgcagcttgg aaaaacggat atattcgaat cattaaacca gctaaagata
     3781 caacaaatac gtgggaacgt gcagacattg acaaattatc tgcaaatagc ggagaaagtt
     3841 atcaagagat attatcacta cctcaataca ataatcaagg tcaagcattc agttaccaaa
     3901 caatcaaaga attacctgta ccaggatacg attctcaaca aatagatgca atgacatgga
     3961 aaaatactaa acaattcaca ccgttaaact tgaaaataac gaaaaattcc tctacaggtg
     4021 aaaaggatct tattggcgct gttttcaaat taacaggaga ttctattgat actttactaa
     4081 cagatcatgg cgacggaacc tattctcttc cagaaaatgt caaattgcaa aaagaaatga
     4141 cctatacgct gacagaaaca aaagctccag aagggcatgg attaagcaaa aagactactt
     4201 gggaaatcaa gatcgcttct gatggtacgg taaccattga tggaaaaaca gtcactactt
     4261 ccgatgatac gatccagttg actattgaaa atccttttgt tgaagttcct gtagcagtac
     4321 gtaagtatgc gatgcaaggg acggacaaag agataaatct taaaggagca gcattttccc
     4381 tacagaaaaa agaagcaaat ggtacttatc agccaattga cagccaaaca acgaatgaaa
     4441 aaggtcttgc cagttttgat tcactcacac ctggtaaata tcgagtcgtt gaaacagctg
     4501 gtcctgccgg atatgatact tcgccgggaa attatgaatt ccaaatcgat aaatatggaa
     4561 aaatcattta cacgggaaaa aataccgaga tgacaaataa tgtatggacg ctcactcatc
     4621 aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa agaagacaac ggacagacat
     4681 taaaaggagc aaaattcaga ctgcagggac cagaaatgga cttagaatcg ccaaaagatg
     4741 gacaagaaac agataccttt ctattcgaaa atttaaaacc tggaacttat acgctgaccg
     4801 aaacttttac accagaagga taccaaggtc taaaagagcc agttactata gttatacacg
     4861 aagatgggtc aattcaagtg gatggacaag atcatgaatc tgttctgtca ccaggagcca
     4921 aaaacaacca gatttcttta gacatcacga atcaggcaaa agtaccatta cctgaaacgg
     4981 gaggaattgg ccgtttagga atctatctag tagggatgat tggttgtgcg ttttctattt
     5041 ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat atgaaaaaac ttggttggct
     5101 tagtatgtgt ctcttcttgt tactatttaa accagctttt actcaggtag caacagaaac
     5161 agaaacagaa atggttcaga ttactttaca caaattgctt ttcccaaacg ggcaactgcc
     5221 gaaaaatcat ccaaatgacg gacaagaaaa agctttatta caaacgtatc gaggattaaa
     5281 tggtgtcaca ttccaagttt atgatgtcac agattctttt taccatctac gggaaaaggg
     5341 caaaacggta gaagaagcac aagcagagat cgcaaaaaac ggtgcgtctt ccggtatgtt
     5401 taccgcagaa gcaacaacta caactcttaa caacgaagat ggtatcgctt ctttttctct
     5461 ggccgctaaa gatcaagaaa aaagagataa agcgtatctt ttcattgaat ccaaagtacc
     5521 agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt cttcctgtac atggacaaaa
     5581 caatcaaaaa ctttcaacta tccatttgta tcctaaaaat gaagaaaacg actaccctga
     5641 tccacctttt gagaaggtat tagaagagcc tagaaatgat tttacgattg gtgaaaaaat
     5701 cacttattcc ttgcatacga caattcctgt aaatatcctt gactatcaaa agttcgaatt
     5761 gtcagatagt gcggatgaag cattaacgtt tttacctaat agtttaacga tttcatcgaa
     5821 tggagaaaag ctgacagaag gctttgtcat acacaagaaa cctcacggat ttgatgtttt
     5881 attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa ctgaccattt cttatcagat
     5941 gcagctaagc agtacagcac aggcgaacaa ggaaatcaac aacaacggaa cactggattt
     6001 tggttttggt gtcagtacaa agaaagtctc tgtatataca gggagtaagc aatttgtcaa
     6061 aatcgagaca aataaaccag ataaacgatt agctggcgca gtattcctta ttaaaaacaa
     6121 agcaggaaat tacctccagc aaacagccaa cggatacaag tggacaaaga acgaatcaga
     6181 tgcgcttcac ctgatttccg ataaaaatgg cgctttttca atttccgggt tgaaaacagg
     6241 aagttatcga ttaaaagaga tcgaagcacc ttctggttat attttaagtg aaacagaaat
     6301 tccgtttacc atttcaactt ttctttctga ggataaagag gcggacagta tattgaaagt
     6361 agtcaataaa aaagaaaata gccgtccatt tcttccaaaa acaaacgaaa cgaaaaatac
     6421 acttttaggc gttgttggta tggtattcgc aagctttgca atctggttgt ttatcaaaaa
     6481 aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa acgttatgtt aggagtcctt
     6541 ttccttattt taccattact cacaaacagc ttcggcgcaa aaaaagtgtt tgcagaggag
     6601 acagcagctc aagtcatcct tcataaaaag aaaatgactg atttacccga tcctttaatc
     6661 caaaacagcg ggaaagaaat gagcgaattc gatcaatacc aaggattagc cgatatttca
     6721 ttttcagttt ataacgtcac tcaagaattt tatgcgcaac gagataaagg agcgtccgtg
     6781 gatgcagcaa aacaagcagt ccagtctttg actcctggta caccagttgc ttcaggaacg
     6841 acagatgctg atggaaatgt cactttatct ttacctaaaa aacaaaatgg gaaagatgca
     6901 gtctacacga tcaaagaaga accaaaagac ggagtgtcag ctgccgcaaa catggtttta
     6961 gctttccctg tatatgagat gatcaaacaa gcagatggct cttataaata cgggacagaa
     7021 gaactagata ctatccatct ctaccctaaa aatacagtcg gtaatgatgg aacgttgaaa
     7081 gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg gagcagaatt tattatttct
     7141 aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa gtgtcacaga tggattgtac
     7201 acttggacaa ctgatcaaac caaagccaaa catttcatta ctggtcattc ttatgacatc
     7261 ggcaacaatg actttgccga ggcatctatt gaaaaaggcc agttgatcgt taatcattta
     7321 gaagttggaa aatataattt agaagaagta aaagctcctg ataatgcgga aatgattgaa
     7381 aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc aaacaccagt agaaaagacc
     7441 atcaaaaatg atacgtctaa agttgataaa acaacacctc aattgaatgg aaaagatgtc
     7501 gcaatcggtg aaaaaattca atatgagatt tctgtcaata tcccattagg tatcgctgat
     7561 aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta tcgatactca tgacgctgct
     7621 ttaacatttg ataatgattc ttcaggaacg tatgcttatg ccttatatga tggaaataaa
     7681 gaaatcgacc cagtaaatta ttctgtcact gagcaaacag acggattcac ggtttcagtt
     7741 gatccgaatt atattccttc attaactcct ggcggtacat tgaaattcgt ttactatatg
     7801 catttgaacg aaaaagcaga tccaaccaaa ggattttcta accaagcaaa tgtcgataac
     7861 gggcatacaa atgatcaaac accaccgtca gtcgatgtcg ttactggggg caaacgattt
     7921 gttaaagtag atggtgacgt tacatcagac caaacacttg ctggagcaga attcgtcgtt
     7981 cgtgatcaag atagtgacac agcgaaatat ttatcgatcg acccatccac aaaagccgtc
     8041 agctgggtat cggcgaaaga atcagcaacg gtttttacaa ccacaagtaa cggtttaatc
     8101 gatgtgacag gtctaaaata tggcacgtac tatctggaag aaacgaaagc gccagaaaaa
     8161 tatgttccat taacaaaccg tgtagcattt actatcgatg aacaatctta tgtaacagca
     8221 ggacagttga tttctcctga aaaaatacca aataaacaca aaggtacact tccttcaaca
     8281 ggcggtaagg gaatctatgt gtatatcggt gcaggagtag tccttctact gattgctgga
     8341 ctgtactttg ctagacgcaa gcacagtcag atttagtctt tttgcaagaa ttggaaaaag
     8401 gagcagcgga ttacgcaaat gctccttttt cttttattat caaacagtca tcacaaggag
     8461 aacacaaatg aaaccaaaga aaaggaaact tctagatctg ctgatgcttt tcttgcttct
     8521 ctcaggaata gggatactag cttatccatt tgttagcgac gcgctgaata attatctgga
     8581 tcagcagatc atctctcatt atcagcaaca agcggtcaaa gaaaatgaag aagtcatggc
     8641 taaaatccaa gaaaacatga caaagaaaaa ccagcaatta gcaaaagaag gtggaaatcc
     8701 aggtgcagat ccctttacta agaaaaaaga acctgcgaaa aaggatagaa cctattttga
     8761 gaaacatacg atcggtattt tgacgatccc tgagatcaat gtccatttgc caatttttga
     8821 tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta ttggaaggaa cgtcttatcc
     8881 tacgggggga gaaaatacac atgccgttat ttccagccat cgaggactgc ctcaagcaaa
     8941 gttgttcaca gacttaccga aactgacttt aggtgattct ttctatatcg aaatcaatgg
     9001 acggacactc gcttatcaag tcgatcagat caagacggta gaaccgactg atacaaaaga
     9061 tctgagaatc gaaaaagggc aggatttcgt gacactccta acttgtacac cttatatggt
     9121 caacagtcat cgcttacttg tcagagggca ccgggttcct tatcatgcaa aagaagttca
     9181 aaaagaagta cagaaagtat ccgagaacaa acgatttttc ctgtatggat tgttagctgc
     9241 atgtatactg attttcctcc ttcttatata tctttttcgt caatcgttca aaataagaaa
     9301 cagaagttag ctattcctca tttctataat atatctattc gaatcgttcc gtaaacaaac
     9361 aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat gatgatatca aagagagcct
     9421 atcaaacact atctttctta ttactaaatg aggatctttt cttaagtttt ccgatattct
     9481 ttaagaaggc ctttttgcat caattcttgt ttgtttttca agttgcggat attctcttca
     9541 aaattttgag aatatatcac tgagaataac gtatttaata cgtaatcgaa ggcaatttga
     9601 gaggagaaag tcgctacttt agcaaaatca tattcctcct gcgatataac aagacacata
     9661 tcagcctgtt tggccatttc tgaatgctga tttccggtaa tcagcaaggt aggggcctct
     9721 acatgcttca aataggtcat tattcgttca taatgatgga tgcgaccgct gtagctaata
     9781 aaaatggcgc aatcctcgct agtcaaattg gctgcattcc aagaagaatc actgtattct
     9841 tcagccatga tcaaaaactt attcaacttg accattttat tctggaattt ccttgccgtg
     9901 atttgagaat caccttttgc aaaaagaaag attcgctgtg ccttgttcaa tactttaccg
     9961 atttgatcta aatcttcatg ttcaatctgc aattgtgctt tttttattga ctggatcgat
    10021 aaatccgcca ttttctttgc aatttgctga gtgctatctt cacttgaaaa aggaaaatta
    10081 ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa cttcccgccc cagctcgaaa
    10141 cggaattctt taaatccgct gaatcccatt tttttgcata aacgaatgat agctgaatgg
    10201 gaagtatacg tagcttttgc taattcttca atcgtcatgt agactgcagc ttcgatattc
    10261 ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa acaactcttg ttttgttagt
    10321 ttttcttcaa taagcatgat ctgacctcca aattctatct tactgataaa cacttggttc
    10381 cgctactatc tttttgattt ttatccttac tttgaacatt aatttcacca aaccgaatga
    10441 taacaagtgt ttttctgaac ttttttacca gaatagctat tataaatact ttctgcgctt
    10501 ttctaggata tactgaaaac atcaataaaa ggaggcacca ctcatgctta caatcgctta
    10561 tcttggcaat ggaaagagta caaatcgcta tcatttgcct ttttcaagaa aacttacgaa
    10621 caaaatcaat atcaaaaaaa ttttttcccc atctgaacca gtgtggaaaa cattcgaaga
    10681 catcgaatat actgatcagc tgtctgatct ttgggatgat cctgagatcc aattagttgt
    10741 catcactaca cctagtcaat ttcattatga atatggaaaa atggcactcg aacatggtaa
    10801 gaatgtacta gtagagaaac cttttgcaga aacgtcaaag gaagcagaag agttatttgc
    10861 attagcaaaa gaaaaaggtc tatttatcca atgttaccaa aacagacggt tcgattctga
    10921 cttcttgaca gtccaaaaag taatcgaaag cggaaaatta ggggatattt tagaagtaga
    10981 aatgcattat gattatttca gaccggaagt accagaaaat acgcatgaat tttctactgc
    11041 gaacagctat ttatatggac atgcttgtca tacagtagat caagttcttt cttatttcgg
    11101 cgaaccagat gatatcagct atgatgtacg tcaactttta gggaaaggac gaatgaatga
    11161 ttacttcgat ctggattttt tctatggaaa tatgaaaatt tcgataaaat ccagttattt
    11221 ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa aagggatgtt cgtgaaacaa
    11281 acaaaagatc gacaagaaga acatctgaaa atgttttaca tgcctgatca tgaagacttt
    11341 ggtatcgatt tgcccgaaca ttatggtgta ttgacctata tagatgaaca agggagttat
    11401 catgaagaaa aagtaatttc cgaggttggt gattacagcc gtgtctacga agggatttac
    11461 gagacattag ttaatggcgc agaaaaagtc atcaaagatg aagaaacaat cttacaaatg
    11521 aaaattttgg aagaaggaat caaaagcatt caagaatcag aaggaaaaat atgacatatt
    11581 tgtaagaatc gggtttgttt tttgacgcat aaactgtctt attgtacgat gaaaaagtat
    11641 agaactacta ggaggaacca acatgacaaa ttatgacggt gaaaaacaca aacgattaag
    11701 aggttcaatg gcccctaaaa cagaagaaga agtggcttta gcaggaacta attctggtga
    11761 aacagaagac aaagaagaat cattagctgg cacaaactct ggtgaaacag aaaataaaga
    11821 aacagcttta tctggttcaa attctgtaga agaggaaaca aagcctaatg agcccaatca
    11881 ctttgaaaaa gatatcgaaa aagcggttga agatgtcaaa gaaaaatttg aagaactgac
    11941 ctcaaaaaat aaacatgatc atcattgatc ggacgaaatc aattcataaa agcaggagac
    12001 atttggtcac ctgctttttt tctgtatcca ttcgatagaa atcgttcggt acagtcgtaa
    12061 aaaaaatcca actgtaagtt gtaccaaaca cccctcgatt taaaagcgct accatactag
    12121 aatataagta ggaggtgaac agcgctgtga actacaaaat tagttatctc ctgaaaacac
    12181 caagagaaat cagtctagaa aaagcagcag agatcatcga agtaaataca agcgaggcga
    12241 tcaaacaatt aactcctttt gccgaatatg tggatgatac ccgtatccgt ataaagaaat
    12301 tccgagcaat cgactgggca gaaaaatttt tggatattac tcgatttgaa gctgtgtata
    12361 ccgaacaaga gcgaagagat cttctttatt tgatggttta ttcgactgca acgtctgttt
    12421 ctgtttttca ttttcaagat tttcttcagg tttctaaagg gacagtcctg tcagatatca
    12481 aaaagataag aaatgaatta gaagtggaag attgtcagct gtcgtatact aggaaaactg
    12541 gttttaggct gctagggtca gaagcaatat taagaagaga agcaaaaaat cgcactgctc
    12601 gtttgatgca gtcacttgct ggaagatttg ggctcacgta tttaaccgca accctttcca
    12661 ttgattatta cgccaaagtt agagatttgc tagataaaac gatttcacta tcagatcttc
    12721 gattcgcacc aaatcgaatg gatgaattag cttattattt gagtttgaca accattcgaa
    12781 ttggaaaaac atccatggaa ctggtaaaag atgctgaact gttggcttct cttgcagtca
    12841 ctgacattag tcgacaagta ttgactggcc ttggctattc cgaacatcaa aattcggaga
    12901 ttatattttt ctctctttgc ttagcatcga tcgttgaagg agatgtccag gagcctgcac
    12961 ttgatttttt gctggaatgt tcttcagaag tcatccatcg catggaattc ctgatggccg
    13021 tagaatttgc ttcgtttcgt gaattattga tgaatgtctt ttatcacttg gtccctgcct
    13081 attttcgtat ttcctattct ttttatttac ctaatgtgat gatcgatcaa attaagcatc
    13141 aatatgcttc catctacgaa atgacaagaa aagcattact tccgttagaa agacgaatag
    13201 ggaagagcat ccctgaagaa gagattggtt ttttcactat actatttggc ggagagatca
    13261 gaaaagcgga cgcagaagaa agaaatagaa aaataagagc cgtgatcgtc tgtccaagtg
    13321 gcattagctc ttcattgatc ttaaaaagtg aacttcagca attattcccg atgatctttt
    13381 ttacagagac taattcagct gaccgcttac acgaggttga cgaaacttct tacgatatta
    13441 ttttttctac tgtacctctc acacttactg ataagaaaaa gtctctctat gttctgcatc
    13501 caatcatgac tgacttggaa aaaaatcagc tgatcaatca agtacaaaga gattggctga
    13561 taccaggatt ttcaatgcct tctgcccaag aattgttgtc ggcgctactg ccttatatcg
    13621 aattaaaaga aggcgtagac gaagaaaagc tctatcaagt attaaatcgt aaaatgaaca
    13681 aacttttaga aaagaaggag gatcaacgtc ctatgctatc tgaattatta acagaagaaa
    13741 tgatccaatt atcggatcaa ccaaaaaact ggcaagaagc aatcacattt gccgcacagc
    13801 cgctattatc agaaaataaa atagaagaaa gttatatcaa agctatgatc gagcgcgtgg
    13861 agcagtatgg tgcgtttatc catattggcg atcacatcgc attacctcat gctcgtccag
    13921 aagatggcgt aaacgaagta gggatgtctt tgctgaaact tcaacaacct gtggatctgc
    13981 ttgataatcc agcccatccc attagtttgt tcatctgttt agctgctatc gataatgagg
    14041 cacatcttcg ggctttggca aatttaacaa agctactttc taataaacaa aatcttcaag
    14101 atttattaca cgcatcaaca aaagaagaaa tattagcgat cataagaaaa ggagacgaat
    14161 gatatgaaaa tttttgcagt atgtcaaagt ggattaggaa caagttttat ggtagagatg
    14221 aacatcaaac aagcacttgc agatcttgga gcagatgtgg acgagtttga agtaagtcat
    14281 acggatgttg gaagtgtatc tagcgatatg gctgattatt tcttcattga gcattctttg
    14341 aaagatacgc taacttctat acctgatgaa aagctcgtac ctttgcaatc gatcattgac
    14401 agcgaagaag taaaagaaaa agtaacgaag atcttgtcaa aagaataaaa atgattcaag
    14461 ggaggaacta tcaatggata aattgttgaa tttgtttgtg tcaatcgtca gtcagccctc
    14521 catcctcgtt tctttgattg cactgcttgg tctcgttctt cagaagaaga aattttcaga
    14581 agtgatccaa ggaactatca aaacatttgt cggattccta gtactgatcg gcggtgctgg
    14641 tttaatcagt aattcactaa ccccttttgc ttcgatgttc caaacagctc tgcatactca
    14701 aggagtagtt cctagcaatg aagcagtagt ggcgattgct ttgcaacaat acggtacacc
    14761 tactgctttg atcatgctag tagggatgat cgtgaatatt ttacttgcta gattcacaag
    14821 atataaattc atttttctta caggtcaagc aatgctttat gtttcttgtc taacagcagt
    14881 cattttagta agcgcggggt ttactgcaaa tttgtggatg atcttattag gcggggtctt
    14941 cgaaggaaca ctattaacta ttacgcctgc tctttgtcaa ccgtttatga agaacatcac
    15001 tggaaacaat caagttgcgt tagggcatac cggaaatatc ggttatgctt ctagtggatg
    15061 gatcggaaag ttttttggaa acaaagaccg ttctacagag gacataaaga ttcctaaaag
    15121 tttcagcttt ttgcgagatt ctactgtatc gatcatgctc ttgatgtcga tcgtatatgt
    15181 cgcacttgct ttgttagctg gtcctggatt tgttgaaaga gaactaagca atggagacaa
    15241 cgctgtaatc tttgcactga tccaagctgg aacattcaca gctgggtttg ttatcgtcct
    15301 tcaaggggtt cgaatggtgt tggcagaaat cgttccagca ttccaaggga ttgctaaaaa
    15361 actcgtaccc gattctaaac cagcattaga tgttcctatc gtttttccat atgcacctaa
    15421 tgctgtatta attggctttt ttgttagctt ctttgtagga acactatcca tgtttgctat
    15481 gataggcttg caaactacag ttatcattcc tggggtagta ggacatttct tctgcggagc
    15541 tgcttctggt gtcttcggca actcgactgg cggtagaaga ggggcggtat taggttctgc
    15601 tttcaatagc ttattgatta gttggctgcc attatttatc cttccagtat taggagatct
    15661 taaaatcgct tcttctacat ttgccgatac tgattatcta gtaccaggac ttgtacttgg
    15721 gaaactagga gaatacggtc aaatagcctt gacagttgga atcatcgctt tctttttact
    15781 tgtcgtatta tctagctttc tttttgccgg aaaaagaaca aacattgttt ctaaagaaca
    15841 gaattaagca aacttcaaaa taaaaattgc ttggacaata tcagaagaag atttaaagtc
    15901 ttctgatatg tcgcaagcag ttttttgttt tatgcattta tttttgaata gcttgttaag
    15961 acaagtggct gttttgctag aagaggataa cttttaccat tcattttcac tgttccgcct
    16021 gttctatcag aagtaatcac gatcttatct tttgtttttt caagcgaaat ctttccatct
    16081 ttgataggta gcgtacaatt cagtgtttca aatgaatcta gattaggttg tatcataaat
    16141 tcttcataac ctggctttgt tggatacaaa ccgataaaat agcgtcctaa aagatagatt
    16201 gggcttgctc cccatgcatg gcataagctt tttccataag gatctccata catactgtac
    16261 ttttcatttc ctgtttgagt agggtcgtat tcttcccaga aagttaccgc atcatgagat
    16321 aacatccctc cccaataatc aagaatcgtt tggtagacga tattcgtctg tccgagtttg
    16381 cataaagcat cttgttcata gaatttgaaa tatggagtag taatctgtgt gatagtgtca
    16441 ttcaataaga cattctttaa gatcgattgc tgttggtctt tgttagcaaa atcgaataaa
    16501 acagcgaaaa tattcgcatg acgcgtcaca tttcttctcc cagattcata gcaatcgata
    16561 aaagcgccaa gttcttcatc ccaaaagtag tcaaataggt tttgtttcaa tagttgatat
    16621 ttttcttgat agagatgagt atgataattt tcgttctctc catatgtttc agctaagata
    16681 tctccgcaag tgatcatgga atgataggct ttcagtaata agacttgttc tgcagcaagc
    16741 gttccttctt tatccatttc agaccaatca ataaatatcc aatcttttgc tctcccataa
    16801 ataaacccta attcattcgt ttgctccatc aagtatctca ttaatgattg catcttaggg
    16861 tagacgatgc gtaaaaattc atagtctttc gtcatcatat agtggttttc gataccaatc
    16921 aaccacaaga tcgaatagtc aacgatagta ttcatatgct gcttgatttc attttgtcct
    16981 cttaatgcta atatcgttcg tttattgatt tcctcgtcaa aaaaaagata ttgattgatc
    17041 atgaatgatt gatacgcatc tccagaccaa atccagcgat ctcgtttgat accgtcgata
    17101 aaaaacaaat cactacataa cttgaaggct tcttctgata ctgaccagat ctgattcagt
    17161 aaagaagaat cgcttgaaaa agcagcttgt ttcgaaagag ataaaaattc atgaaaaact
    17221 tgaagttgaa ccattccagt agaaacctct ggtataaaca aatagcgaaa agcgcgttta
    17281 cgagctttca tttttcttgt cattcctatt tgcttatagt agcaataatc gatatccata
    17341 gcttctacat cggactctcc ataacataga gtgatagatt cgatatggtc atccagagat
    17401 attagttcta cacagccatt cactgctcgc ccaaaatcga ataagattcc tccgccaatc
    17461 tccgtttcag tgactggatg gacgcattct ttttgataat agatttgatt tggatctttt
    17521 gcgatatcga cataaagcgg actccagcca gctggatatt cttcaataaa attacttgct
    17581 gtccagccaa tatctgattt tacttcgtct ccctcgataa acatcgctgg tagccctgat
    17641 gtatgtccta caaagatacg aatttttgct ttaccagctg gacaagtcag ccattttcca
    17701 catggatgtt tttgtccatt aatttctaca tacccaactc cttggatcgt cactttaaac
    17761 atggaaggtt gatcaagaaa gtaatatctc ttaaacttga cgttccgatg gcaatcatcc
    17821 atgtaccagt acgctggcca gtcaaaacct cgttcttccc gttggaaatt ttgtagcagt
    17881 ccatggcgga tctcaaagtc tccaggatac caaagccatc tatctttatg tttttcattt
    17941 gtcattttac acgacctcca tcaaatttat tttgttgtca attgttcttt tatttcttca
    18001 tctactctta tcactgtctt tctattcgtc ctgtgtacta ctagaaggct aataagcgcg
    18061 ctaatcaaca gcaagatact gccattgacg aaaacagcaa gtgttttact cccaagagaa
    18121 gcaaaagaga gaaagagcaa accatatggt gcaatgaaag tacctaaatt agcgccaacc
    18181 aataagattg aagtattgaa actctccgcg tacttgggcg cttggatact tacttggttg
    18241 aataagtaag gaatgaataa cgaaaaagaa attcctgtta ggatcgcacc aaacccaatc
    18301 acatacaggt ttatcacagt ggcaatcaat acaaagccga ttcccatcag tagtaaagaa
    18361 agtgtaagca tatagcgatg tgcttttgtc tgaagccagc caaaacaaac tccggcagcc
    18421 atacttgcca attgcatgat tgttaagata ttcgctgcat tagtttcgtt tcctagagaa
    18481 taagtcgtca ggactgtggc taatttaatc gatacagcat tatataccac gatgaccagc
    18541 aagagaaaaa taccataact aatcatttta ggattcgttt tgattacttg tcttgttggg
    18601 gctgtgccat gcgattgaag ttcagttgtt tcaggtgtag gaacaaacaa aacaaacaag
    18661 acaagaatag gtaagctgat ggcgtagact aaaaaggtat attgccaacc gaactgtact
    18721 agctgtcctg caacaaatgt cattaaagct gcaccaaatc cttgaaatgc actttgaaaa
    18781 ccaatcatag aggctcgttc atctccgtga taaaaggcag caatcatgct gactgccaaa
    18841 gaattgatca atccaaagcc aatacctagt ccagctcttg aagctaagac aatactgtaa
    18901 ctttgtgaga agagggggag gataccacaa atcgatgcaa tcactaatcc gatgatcaca
    18961 gtttgttttt gtccgatttt cctagcaata aaactactaa tcaaaacaaa aatagtgacc
    19021 ataaaagagg gagcagtcgt taataattca acagcagata aagaacgcgt ggcataagta
    19081 gctgacatac tgggaattgc agacgtgact gcaccagcag aagtcaaaat aagagagact
    19141 gacagcagag aaaatttcat catcttttta ttcataagca tgttcctttc atttttgtaa
    19201 gcgcttatca aaaatatagt atgaagtggt tattctctca atagcggaat cacaggaagt
    19261 tcatgtccca accatagaaa gtctttatga aaccaaaaat agctttgcat ataatagcag
    19321 tacagcgtga actaataagc gaaagaaacc gcttacaaaa aggagggagt caattggaac
    19381 tagctgaatt agacattttt ttgcgtaacg aagaatcaat cgaaactctg cagcgtgaac
    19441 aaggaatgaa tatcaatgat ttaaacttag cttaccatct tcagacaccg ccaaaaatcc
    19501 accgagggga tctatttcaa cagggaaata ttcatatcag taaacaccgt cgatatgcgg
    19561 aagttcccga gcatacacat gattttgttg aaatcaatta tatgtatagc ggtacatgtt
    19621 tacaaattat taatggagaa aggatagaga tggaagaaag tgacgtggtg atcattgata
    19681 aagaagctac tcaaagaatc gaatacacag ggcaagagga tattttaatc aacatcttat
    19741 taaaagaaga aaccttatct acagagatat tgaatcattt ggcaagcaca aataatctac
    19801 tttcttcttt tttgataaat gcatcaaaag aaaaagggga acatacacaa ttcattcatt
    19861 ttcgcggcgg aaaaacaagt cgtttgacgc atttgattga aaatatgctg ttgatttatt
    19921 ttgatacata cagtcataag atccgttcgt taaatctcta tacatcacta ttactgatcg
    19981 aattgactca tttattagaa caacaaagct tttcatcagc agatggatgc gcagaaaatg
    20041 agatgcttag agtattagat tatatcgata agcattttac ttcttgtacg ttaaaagaaa
    20101 cagcagctca ttttggctac aatccggtct atcttagtaa taaattgaaa atgtattttg
    20161 atgcttcctt tcaagaactg gttctaaaga aaaaattcga ctttgcgttg gaactgattc
    20221 aagaaacgag tttttcgttg gaagaaatcg ctaatcgtat cggatataaa caaacgactt
    20281 ctttattcaa attattcaag aagtattcaa catgtactcc caatgaatgg agaaataaac
    20341 attaagaaga aaatctttca ctaaagcata tgaaggaaaa ttgttttagg tgcgattgct
    20401 gtcttattat atcgttgcag cgaatttttt tggatttatt ttcagttcta aacttttggt
    20461 tgtattatca ggggatatag gtgtaaacat ttgtcattta tctgttatgc gtattcagga
    20521 attcgtttcc atcaaaaatt ataataacaa ctattataat taacatgttg ataaaatgaa
    20581 taaacaagac tatacttttc atgaaagtaa caaatagtta tgaaaaggag ctgtttattc
    20641 atgaaaaaaa caagtcgtaa agtagtaatc gtcggtacag gttttgttgg aacaagtatc
    20701 gcttatgcaa tgatcaacca aggaatctct aatgaattag tattgatcga tgtgaatcaa
    20761 gaaaaagcag aaggggaagc attggacctt ttagatggta tggcttgggg cgatgaaaat
    20821 gtggctgtat ggtcaggtgg ctatgaagaa tgtaaagatg ctgatatcgt tgttatcaca
    20881 gctggtatca accaaaaacc tggtcaatct cgtttagatt tagttaaaac aaatgcgtca
    20941 atcatgcgcc aaatcgtaaa agaaatcatg ggatcaggat tcgacggtat tatcgttgtt
    21001 gcttcaaacc cagtagatat tttgacatat atcgcttgga acgaatctgg tctgccaaca
    21061 tcacgtgtta tcggtactgg aacaacattg gatacaactc gtttccgtaa agaaatcgca
    21121 ttgaaattaa aagttgaccc acgcagcgtc cacggctata tcttaggtga acatggtgat
    21181 tcagaagttg ctgcatggtc acatacaact gtcggtggta aaccagtgtt tgaaattgta
    21241 gaaaaagacc atcgtattgc ccaagatgaa ttagatgtga ttgctgataa agtccgtaat
    21301 gctgcttatg agatcattga tcgtaaaaaa gcaacttact atggtattgg tatgagtact
    21361 gcacgtatcg taaaagctat cttgaataac gaacaagctg tattgcctgt atctgcttat
    21421 ttaactggtg aatacgacga aaaagatatc tttacaggtg tgccatcaat cgttgacgaa
    21481 aatggtgttc gtgaagttgt tgaattatca atcaacgaag aagaaaaagc aatgttcagc
    21541 aaatcaacaa gtgctttaag agaagtattg aacactgtat tataagaaac aatgaatatg
    21601 aactaaaaat cctaaaactg cttaacagct gaaatagctg ttaagcagtt ttttattgat
    21661 atctaatagg taagcaaata aatagaatgg ttaaaattaa tcggtccgaa aatgtatccg
    21721 tacatatgaa catatgaaat ttaatctgtt gattacaata agcgaatcgt tatcaatgtt
    21781 tatctgtggt taattaaaaa atccttccgc aacg
//
LOCUS       DAZKOB010000043        20908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000043 DAZKOB010000000
VERSION     DAZKOB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.043"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            202..1071
                     /locus_tag="ACG8M6_001518"
     CDS             202..1071
                     /locus_tag="ACG8M6_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0457194.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            1091..1438
                     /locus_tag="ACG8M6_001519"
     CDS             1091..1438
                     /locus_tag="ACG8M6_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457195.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            1451..2713
                     /locus_tag="ACG8M6_001520"
     CDS             1451..2713
                     /locus_tag="ACG8M6_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457196.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            2793..4169
                     /locus_tag="ACG8M6_001521"
     CDS             2793..4169
                     /locus_tag="ACG8M6_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0457197.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            4166..4795
                     /locus_tag="ACG8M6_001522"
     CDS             4166..4795
                     /locus_tag="ACG8M6_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457198.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            4807..5277
                     /locus_tag="ACG8M6_001523"
     CDS             4807..5277
                     /locus_tag="ACG8M6_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457199.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            5274..5756
                     /locus_tag="ACG8M6_001524"
     CDS             5274..5756
                     /locus_tag="ACG8M6_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457200.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            5753..6415
                     /locus_tag="ACG8M6_001525"
     CDS             5753..6415
                     /locus_tag="ACG8M6_001525"
                     /inference="COORDINATES: protein motif:HMM:NF019467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0457201.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            6402..6770
                     /locus_tag="ACG8M6_001526"
     CDS             6402..6770
                     /locus_tag="ACG8M6_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0457202.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            6851..7297
                     /locus_tag="ACG8M6_001527"
     CDS             6851..7297
                     /locus_tag="ACG8M6_001527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457203.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRITEQAIEKQKDKEKKEIMSIYLSSGASFFHEID
                     SMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            8006..8605
                     /locus_tag="ACG8M6_001528"
     CDS             8006..8605
                     /locus_tag="ACG8M6_001528"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0457204.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            8621..9097
                     /locus_tag="ACG8M6_001529"
     CDS             8621..9097
                     /locus_tag="ACG8M6_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457205.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            9114..10016
                     /locus_tag="ACG8M6_001530"
     CDS             9114..10016
                     /locus_tag="ACG8M6_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457206.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            10089..10280
                     /locus_tag="ACG8M6_001531"
     CDS             10089..10280
                     /locus_tag="ACG8M6_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457207.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            10444..11031
                     /locus_tag="ACG8M6_001532"
     CDS             10444..11031
                     /locus_tag="ACG8M6_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457208.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            11031..11930
                     /locus_tag="ACG8M6_001533"
     CDS             11031..11930
                     /locus_tag="ACG8M6_001533"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFQ0457209.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            11930..12844
                     /locus_tag="ACG8M6_001534"
     CDS             11930..12844
                     /locus_tag="ACG8M6_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457210.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            12896..13111
                     /locus_tag="ACG8M6_001535"
     CDS             12896..13111
                     /locus_tag="ACG8M6_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457211.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            13296..13550
                     /locus_tag="ACG8M6_001536"
     CDS             13296..13550
                     /locus_tag="ACG8M6_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457212.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            13729..14772
                     /locus_tag="ACG8M6_001537"
     CDS             13729..14772
                     /locus_tag="ACG8M6_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0457213.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            14945..15169
                     /locus_tag="ACG8M6_001538"
     CDS             14945..15169
                     /locus_tag="ACG8M6_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457214.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            15172..15429
                     /locus_tag="ACG8M6_001539"
     CDS             15172..15429
                     /locus_tag="ACG8M6_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457215.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            15531..15884
                     /locus_tag="ACG8M6_001540"
     CDS             15531..15884
                     /locus_tag="ACG8M6_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457216.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            15937..16731
                     /locus_tag="ACG8M6_001541"
     CDS             15937..16731
                     /locus_tag="ACG8M6_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457217.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            16760..17878
                     /locus_tag="ACG8M6_001542"
     CDS             16760..17878
                     /locus_tag="ACG8M6_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457218.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            17911..18231
                     /locus_tag="ACG8M6_001543"
     CDS             17911..18231
                     /locus_tag="ACG8M6_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457219.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            18486..19175
                     /locus_tag="ACG8M6_001544"
     CDS             18486..19175
                     /locus_tag="ACG8M6_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457220.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            19212..19379
                     /locus_tag="ACG8M6_001545"
     CDS             19212..19379
                     /locus_tag="ACG8M6_001545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457221.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            19399..>20908
                     /locus_tag="ACG8M6_001546"
     CDS             19399..>20908
                     /locus_tag="ACG8M6_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457222.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNH"
BASE COUNT         8020 a         2682 c         4306 g         5900 t
ORIGIN      
        1 taaaaaaact agctattttt ttaaaaataa tttggttcaa aatttatttt tttaacttga
       61 taattttttg ccatcctttt ttaaattggc tatatatcaa ggtttatagt ggggataaat
      121 aaaaatacat ttgacaaaat ttacaatata gtttattata tatttatctt attatgataa
      181 cacaataaaa ggaatgatgg tatgaaaaag aagaagttta gtcgatcgtt gttaggaatt
      241 ttagttttag ttgcggttgg tcttagtggc aagggttact ttcagaataa cagcactcag
      301 gaacatgatt ttcagatcaa aagtagtgtt caggtaggta ataaagcaga cagtgaatca
      361 aaagaatttt tagggatacg ttatgatcca aacaaatatc caaataatat cgtaaacatt
      421 aatgatggga aatatacact gacaaaagaa gaccatgaag cattatcaga aaaaggcagt
      481 caaacctttt gggtaaatta tgcagattta gatcaacttg gaagagcagg agatgttgaa
      541 gcgttgatca cctatgatgc tataaattct cactcatcta aaaatgtgga acgtccatct
      601 ttcaaaagtt ctgtccatat tgcaggagaa tacaaggatg gagtatttga tcaagagaga
      661 gatacttggg tcggcaaaga ttcaaacaat gaaatcactc agctagaagg atataaaggt
      721 tatctctata acaaaagtca ttcattagca tggtcgctag gaggagatat ggaggtacac
      781 aatttaacac ttggaacgag agcgcaaaat gttgggaaaa atgatcagaa tggagggatg
      841 gcttactcag aaacgattgt acgtgatgca atctataaaa ataagaatga aaaagtattg
      901 tataaagttg atccaattta taaagggaat gaattggttc ctcgtggttc tcatgtcgaa
      961 gctttctcag ttaacgataa tggaaaaacg gtaaacctta atgtttatgt atttaacaat
     1021 caaaaaggaa tcaacatcaa ctatcaaaat ggtacttggg taagagatta gaaattgaga
     1081 aggtgaagaa ttggaaacaa taagcatcaa tggattagat aaatcagttg caaatcgaat
     1141 aaaaaaagga aatggctact atcttctgaa gaagggtaaa tattcagttg ctagagagtt
     1201 agaaacaatg agtatcgtgt atgtagctaa gaatcatttt gacgtagaac aggctatttt
     1261 tgatccagat tctgaaatag ggaaatgtgt tgaattatca gagaaaactg ttagtacaaa
     1321 aggggcgatg atcattgaga gagaaatgtt agacgaaatc agagtaatca ttcctaaaga
     1381 tacgcagatg tgtgaggaca gcataagtga cgatgaacca aaatcagaaa gaaaataagg
     1441 agatattcag atgagcgaaa aatccattga attaaaggaa tataagcgaa gcgatggaga
     1501 aaaatattat cctgtagcca gtttttctaa atcagtatat ctggttggac gtgcattggt
     1561 agttggtgca attggcatat atattttgca ttgggtaaca aaggttgtac aactgcttgt
     1621 agatacctta gtaagtattc ttgtctcgtt aaatgatgtt aacggaacag atgatttagg
     1681 attaaaagaa atgtttaaag agttactcaa tgcgtttttt cccaattatt taagttattc
     1741 atataatgta caaagctttt ttgcaaagct acacttggta gacatctcta ttttagtatt
     1801 agaagcagga acggtattct ttgttggata tttggttact ttgatgttca ggcatcacta
     1861 tggagaacaa agaccatttt ttgatgatat ggaaagtaga aggttgaaaa gagaagctct
     1921 agaagctatg gatgtgaagt atgatccaaa caagattata ggagatgaaa cgaacaacaa
     1981 aaaaagaaaa cgaaacaaaa cgcaattaga atccgatgtg ctatcaacgc ttaaaaatat
     2041 caaaatttct gttcatacaa gacgtgatct aagtgtcaat gaagatattc gagaatatat
     2101 cgtaaaaatc ccgcaaccaa aagggaagaa agttagagaa acagttttac gacaaattca
     2161 agatttaggg tcgatcctga caagagcaac aagcggacga gccaaatttg gtgaaaaaga
     2221 agatgaagtt tcaggcgatt atttcatttt ccgagcatca gtaaatattg aaagagaacg
     2281 taaggaaaga gtaagtacca aaaacaaaga aacggaaaga gcaacatctt caatcgcagt
     2341 ttcaaattca caattttctt ttccaatcaa tctctttgcc gataattcca aaaatatcca
     2401 aaaccaaacc gaaaaagcta gaaaatatgc cgaaactcaa aagcaatctt tagaacttta
     2461 tctatcttct gctgacgtac ctgcaagtct aacagatatg cacgttggga atacatctat
     2521 tgaatatttg tttactttat ctcgtaccag aaaaattcaa ctaagctcaa tgaaagaaga
     2581 aattgaaact tacttgcata tatcaggtgt aatcgtgcgg atcaacgcag ggcgtattca
     2641 aattactcta ccattaggag aaaatgatcg tattccaatt gatgtaccat caatgatcaa
     2701 tagcgtgttt taaaagacat cacaaataga aacagaaaat acaaactacc agttcacaaa
     2761 cagaaaagta ccgagaaaag tgaggaatag aaatggctat gaaattatta gagaaagtca
     2821 aaaagagagc cgaaaaagct gaatctattt tgctagataa agaacctaat ataaagctta
     2881 atggaaaatt cagtgtcaat atgttgaaca caaaggctaa agacccgaca gatgctattt
     2941 ttggaattaa gcctgatggc acaattgctc atttttgcat cagcgaaatg ccacacatgt
     3001 tggttgcagg aacgacagga tcagggaaat cagtattact gaatgaaatt cttgttacag
     3061 caatgtgtca ctcaacgcct gatgaattaa aaataggaat tgtagaccca aaaagggttg
     3121 aatttggaag atacaaaaaa ttgccgttca tgttggctga tccaattaca gatatggatg
     3181 aagcttatga tttttttgaa tatcttgtga tcttgatgca tgaacgttac aaactgatgg
     3241 aaaaagcagg gttacaaaac atcacgctat ataacgaata tgccgaaaaa aatggattag
     3301 atagatttcc ctatgtgatc ttattagttg atgaatattc acagttggtt ggaacacata
     3361 aagaagtaga aggtttgatt gttcaattag gacaaatggc aagggcagca ggtattcatg
     3421 ttatcttagc aacacaatct cctcgatcaa cagttgtcac tggtattatc aaagccaatt
     3481 ttccgtcacg tgtttgcctt atggtagcaa gtgatttaga aagtaggatt gtattagatg
     3541 aaggcggtgg agaaagtcta agtccaaagg gagatatgct tattaagctt gttgatggaa
     3601 gtatggttag agcgcaggga gcttacatca gtaacaaaga aattgaaaca atcttcaatc
     3661 accttagaaa tactatgcca gaaccagaat atgttgatta ccgagcaatc gtagctgaaa
     3721 ctcgtggtga gggcggtgaa gacgaggatt ttggtggaat tggtaaagct cctgtaagaa
     3781 aatctgctaa aagaaatttg aacacacctt tagcaaatcc atcatctaaa gagccatcaa
     3841 ttgaaaagaa gagtgcgttt gctattaagc ttgaaaaaaa taaagaagaa aaagctaatc
     3901 agggagctaa gtcactagaa aaacttcagc aagaaaaatt agaaaaacta gaaaaagcat
     3961 tatcatcatc aacttctgaa actcaatctt caaagggtat agataatgga caatcaatta
     4021 caactaaaaa caagcctaaa aatagtaatt caaatctctc ggtaaatgtt gttatcacaa
     4081 ctgataaatc tgaaaaaaaa caagtgaacg gaactacttc acccatcatg caaaaaatga
     4141 taaaggcaaa aaaacaggtg cagaaatgat tatcattagt tcctttttga gatggctaac
     4201 accatataaa tatacagatg aaatagttga gaagcgagcg aagaaaaaag ttaaaaaaac
     4261 gatgttaact cctaaagaaa tgctagatcg tggaatattg ggagtgaagg ttttagaagt
     4321 aaaccaaccg tttgaattgg ataatgcgag agataggttg cttagagctg aagttgatgt
     4381 cgcatacttt tatcatgata aaagcaagca tcaaatagct atcaaattca atccaaaatc
     4441 ggcatggcga taccaagcaa gagatgacct aaagcgtagg aagctgaaac cgatgctcta
     4501 tcctgaagga aaagaggcat ttgatagaac ccatattgtt cccattggat ttcatggtac
     4561 agagtcagat aaccgcctat tggtagggtg gaacagctca caaaatagag ttcagatcaa
     4621 taaatttgag aagaaggtaa gaaaatttaa cgatcggaaa ataatacttt ggtttgccaa
     4681 tattcagaag caaccagatg ggacagcaaa gtgggaagcg gtcatatttg atgaagatgg
     4741 caaaaaagta atgtctgaag tatggcatga taaagctaaa tttgtttggc agtagaggtg
     4801 aaaattgtgt taactaacct aaaactatat agagagggat ttaacgaaaa agtaagtatc
     4861 ggtgaatcta ttgaatattt aggaaagaag tacattatta ttcgtattct tgaaattaaa
     4921 aaactttatt ttagtaaata taataattta gcagttagag ttctcgtcca aaaggttgga
     4981 aatccaagtg actatagaag tgattaccat aactataaaa cagaatcatc tgtaattaag
     5041 cgttataatc aatcaaaaaa tgatgagaga atattcaaag taggagaaat tattatttct
     5101 aagtatggga tagcatacga aatagtttct attaaaaaat tttattatga atttgttgat
     5161 ttggttgtag aatattctgt tcgattgatc gttccgtgga gtaaagaaga aattaatgag
     5221 gcattaaaac atgaacgaaa aagtacattc aaagttttag aaggtgaaaa aaagtgaaaa
     5281 cagaccttac taggcaagct gagaagtgct tatggcatga aacaaatcgc atgggtgtat
     5341 ttggttgttt cgaagtcact atcggttggg atggcagtga aagagtagat tatataactt
     5401 attcagtaga taacacagtt cgatgttatg agatcaaagt tactttagct gatttaaaaa
     5461 gcaaggcgaa acagactttt atcggtgatt ataactattt agttgttact agtgatttgt
     5521 gggagaaaat gcaacaagac gataaactta tgcggaaata ttactatcaa ggcgtatacg
     5581 tgttcaatga aaaatatcct agcataataa gtgttaagaa agcaaggaaa caaaaagtat
     5641 cgctaggtac aagatcaact gtattagaaa gcatggttcg ttctatgaat cgtgaagtta
     5701 agaaactcta taaagtaaaa ccattttggg agatagcaga ggtggaaaaa ggatgattca
     5761 aacatttgca aaaatgagta gaagtgcttg gattgaaaag ctggaaaatg atttgagtgt
     5821 tatgcgatta gctccaaaga aattcaaacg agagattgca gaaaaagaag caattttgtc
     5881 ggtattgaat gcggtagaac tggacgaacc gaaaaagtca gttgatgtac ctgaaaatgt
     5941 agcagagtta ctagattatt atcgtaattc gtcagatgtc gatttgttag ctctgatatt
     6001 aacatttcac gattggtatt actgtcaaaa tgaagatact cagcatgata aacatgataa
     6061 agcagttgac tggttaatag agaatccaga aaagttcatg cgtgcttggc tagatggcta
     6121 tgatagcaag aaaaagccac tggaaagtaa tgaggaaacg tatttaacgg cagaacaata
     6181 tgactatgct agaaaaacag gagattggga atctggtaag actttcaatc acagaaaaga
     6241 tcaaccaaaa attagttttt cagatatagc cgcaattatc gaaggtatct actatggaca
     6301 agccgatgaa gagatctttt ctatacaaga tttattagaa tttttgaaca aaaataagga
     6361 tagctttgta gaactaactg attatgagga ggatacagca aatggaaaat tatgatccaa
     6421 atatgcgatg gggtacacat acgcttaaag tttcttttca acgttgggat tacaagggat
     6481 ttgtgacttt tagaaaagct ggaaattgta aaggattaga tgtcttagcg ttggatgaag
     6541 actatctata tgaccatcca ttgacggata atccaatcgg atttggattg cttcctgaag
     6601 atgatgaggg caatgaatgg ttcaaaatga ttcttacaaa cgataaaggt gatcaattat
     6661 ttgtagaaga tacttggtca tatttgagtg aatatattgt tagcattaaa atcattgatt
     6721 ttgtagcaga taaagaaaaa gagattggag aaggtaaatc taattactag ctaatgaata
     6781 gcaactttgg aggataagtc aaaagaataa aaaaagaata ctgtgtgtca ttgcagggat
     6841 gtatctttgc atgatagtat ctgcggttac catttattgg ttaggaaatc agcaaaacag
     6901 tttaattgtt gatctggggg agttaaaggt tggattacga atgactagat ggcttttatt
     6961 agctcaaaca gttggtttag ccatattgac ttggatatgt gtggaaaaaa taacatctaa
     7021 taataatcaa gagattgttg aatcaacaaa cctaagaata actgaacaag caattgaaaa
     7081 gcaaaaagac aaagaaaaaa aggaaataat gtcaatttat ttatctagtg gagcatcatt
     7141 ttttcatgaa atagattcga tgaaactgag tagtcaagaa atcattgata gatggaaaaa
     7201 catgcctcta ggtggtgagt taatcgttga agatagtgat ggttggtcag cttataagaa
     7261 ggacgagatt gtagctatca cgattttgtt tgaatagtaa tatattacca ctagataatc
     7321 tgaatatgtt gatctgctag tgacgtacca aatgcgttta atagaacctt ggtcacaggc
     7381 agaaattgat aaagcagtaa aattgaaccg attatcaaaa tttaacgtat taggaaatgc
     7441 cttttaaaaa tcgcaataca caaacagaaa aataccaaaa aattagcaat gaacgtaggt
     7501 aagaacatga caataaataa taataaccct aacaaatata atgctcttgt ccaatgctat
     7561 atcgaaaaca aaattacaat gcaagaagtc gctgatgaat taggcaaatc aaaaagccta
     7621 gtatcacacg actttaagca caatgttttt ccgaacccga ttgagctgag gaaaaaattt
     7681 caaaagagag tttatcaaga aatagggaag tggattgatg aagggaatgc aattgaccct
     7741 ttaattgaaa aatatccttt tttaaaaagc aaaaataaaa ttcaaaggtt aattcgtcaa
     7801 ggcgatattc aagctaaaga acctgttttg acaaccaatg atttggctaa aataaatctt
     7861 cataaacaga ttattgaaga ctttgaaaaa aatctttcaa caactgaaat tttaaaaaaa
     7921 tacaatattc cgagaggaaa gctaacatcg actcttttta gagaatatgg agaatcggca
     7981 gttactaaag caaaggaagt agcgaatgag agaaaaaaac aaaaatagta gtcgatactt
     8041 agtagcgact gtagtttttg tttgtgtatt gattttcctg tttgattttt tacagattgg
     8101 aaaaagtata attggaaata cattgagtgc aaaggaaatg gttgctttag gtggtttgta
     8161 tgaaggacat ttgttcccga atatattcat ttctatcgtt tctcatatta acttgtacca
     8221 tttaatttct aatttattgt tcttagtagt tcttgaagaa gcactgaatg atcagtatga
     8281 gtggttaaca ttattgatcg ttttaatcat atcaggattt ctaggaagct tagttccttt
     8341 tctgctacaa agcaacatcg taagtatggg gcttagtgga ggtatttttg gatggattgc
     8401 tttatttgcg attgcataca tccattttga tagcaaggaa catttattac taagtgtttt
     8461 tgtattattg ataatcggca ttttattggt atctactttt gaatcaggcg aggcaaatgt
     8521 cttgtcacat gtaatcggat ttgtctgtgg aatgatcttg ggatttattc taaagaacgt
     8581 ttcattgatc aaacaaaaga attaaaaggg agtgctaaaa atgccaaaaa gaaaagaaaa
     8641 gaaaattcca gtgtttttgt ctgtctcaga aatgttatca cttcaaatac tgttaagaaa
     8701 aaagattgaa acaatagacg aagaagttag aaataaaagg gctatgtacg aacaacgtga
     8761 agcgctaaga gaacgaatcc actattttga tgatatacac actaaaatct atgaagcacg
     8821 tacaaatcac gaagacgaaa gtaaattagg attagctcaa caacggatac ttgaatggct
     8881 taatacgaat actgatcaag aaccgttaag agtgattagt aatctttttg aatttggaac
     8941 gaagcattgg tttaatgctg tagagaagga tgctgactta caagaagcgt ttgatacgct
     9001 aagtaaggat agcaaatcca tgcgagattt attgattact tatctaaatg agcatgagtt
     9061 tgaacaaacg aaaaccaaaa agaaaaataa agaatagaaa gttgtgatga atcatgctat
     9121 taaccctgtt aggttattta tgtttaattc ttgctttttt tggggcaatc gctataatta
     9181 ttggtcaatt tcaaacaaat aatccgatta tgcgtaacat aggcattgga attgtgattg
     9241 taacctttat tgtgtttaat gccactaaga tttttggaag taacgataaa caatttggta
     9301 tatcgggagt taataatagt tatgctccag attctttggg aagggtagta atagatggaa
     9361 catttgaaaa tgtaacgagt tataaaaatg tttcgctgac agtgaaggaa gatggcaaga
     9421 aagcatcaga cgacgttctc gctactatta agccgtctca atcacaattt gaagtgtttt
     9481 atacagttaa agagaatagc cctgtacaca agctgacatt ggtttttaac gttgatggag
     9541 aagaatacta taaagatgta aaaataaata aaacatcaac gaaaaagatt atcgaatcag
     9601 cgaaaaaaga agtagctaca gaagaaagta caactgaatc aagttctact gtcgaagcgg
     9661 aactaccaga taaaattgat tttgatcctg aaaaagtcaa atcgtttgaa tctgtttctg
     9721 gttctatggc tgatcggttt aacactgaag tcttcaaagc tatcaaaact gttaaattgg
     9781 atactgaaaa gcaaatgatt aatgttacct tattcaattc agttgaatct atggatcagg
     9841 ataaaaaagt tacggttgca agagaactag gaaaaagagt aattggtttg tatcgtggta
     9901 ctgctaaacc agattcagac ggtgcagatt tgccagttca tgtagtttat gaagacggta
     9961 aagtttttgg agattctaca gcaaacgaag cattaacgat taaattggct aaatagccag
    10021 aaagttataa taaacaaatg gtgtcacacc aataatatat aggtgtgaca ccttggagat
    10081 gatagctgat ggtagtaaat aaagaaaaag ttagaaagta tgtgacttta aaaatagctt
    10141 ctgttaaata tttagaaaag cgagctaaag aagaagaaag agcaagaggt aagaaagtaa
    10201 cagtttcaga gttgttagaa gaattgatcg agaatgatca aacaataaga cgtgcattta
    10261 ctaataggtc tatcagataa caatataatt aataaaaaag aaagagtgat ttggtcttag
    10321 actaaatcac tctttttata taaagtttct ttacactcat tccgaataat gttaaaattc
    10381 tttatatgat atatagttcc ttgtcagagg aagagataac cagcaatatt ggagggataa
    10441 taagtggaaa acaataatga tattagacat tttagcattg gggaaagagt tacaacagta
    10501 agtgaattat caaaggatta tagagataaa gagggaacga ttatttctct taataagaat
    10561 cgaagaggcg tttggcaata tgttgttgat ttaggtgatg gcgttacagt tgagtgtaaa
    10621 gatagtcaac tgatcagaaa taatggcagt cattccattg aaaaagcaat tcttgtatac
    10681 ccaaaagtat caagtaagat catgaatgac tggttgcttg gaaaatttgg tgagtacaat
    10741 aaaaaagaaa tcgcagaagt gatcggcaaa tcttatccaa cagtattagg gtatctaaaa
    10801 aagataacga gtaaagaagc aaaaattgag gatgttttaa cagtaagaga gcttcaatca
    10861 ttaatcgagt atcggaataa acaaataaca aatcctgagt accttagaaa agaagatggt
    10921 ctattgaaac aattggaaca agtaaataaa atccggaaga tgcaaaaggt agctccttta
    10981 agccttgata cctttaaaaa aatctatttg aaagagatca aaaaagacta atgtggactg
    11041 taactttacc gttccaacct gtatctgcac caagaccaag tgcaagaagg aatgctgctg
    11101 atgaaactaa tgacgtagca gagaaaagga tttcaaccta ctatgataaa aagtatcttg
    11161 attatttgaa aaaaataaag agctttatcg aagatcaaga tttattagat gatgaatttt
    11221 actcgattgt aaatgatcct atgggcgcaa ttatggaagt ggacttttac taccagatac
    11281 caaaaagcta taaaaaagtc tataacatta tgaagacaac agcgccagat ttagataatt
    11341 tagtaaaaag tgctatggat ggtattttta acatctcagc tatacgagat agccatatca
    11401 caggcttaat tgcttttaaa tataatgtgg cgaacgaagg aaaaacagtt gtgaggatca
    11461 aacgatattc agaatttgag tgggatactt ctgaaggatt gaacggagat atatgggaag
    11521 ctacatttga ttttaaacca gtagcaacgc caagaccaaa gtctaaattt agaggaaatg
    11581 ggattatcac ttactatcca aaagagtaca acgactattt ggaaaatatc aagaatacgc
    11641 ttaaagagaa agatttggta aatgatcaac tgaaaaaggt aatgaatgct cctatggggg
    11701 ttatagctca gatagattat ttttaccaag tgagaaaaga taagaaaaag cttgattcac
    11761 tgatgcgtac ttcacagccg gatattgata atttagtcaa aggaacattg gacggaattt
    11821 ttaacaagga aataggcatc aaagatagca gggttgtagg gttaattgct tttaaataca
    11881 atacttttga aaagtcgaaa acaaacgtga gactacagga gatgggctga tgaataataa
    11941 tgaaaaaatt cttcatgtac ttgatagctt tgaaataatt caagaagaac taaaaaaata
    12001 tcgggatgtt ttggaacaaa gatatgattt tgtcaatcag caaaagagta atcacatgga
    12061 tttcattctc aatatgaatg acttgaagaa gaaattagtt gaacgaaaag aacaagaaaa
    12121 gttgatcaaa gcttactttg aactcggtga aaaagaagtt aaaaatgcaa tggaactgaa
    12181 tgaagagcgc agggttttag atcagctgtt agaacaatta ttggtgatgt ttcaaaaagg
    12241 acgaatagat gaggatttaa ttgaagaagg attgagaaaa tatccttcaa actcaggtat
    12301 cggaatagtg ctgaaagcga ttgatgaaga agaaattgaa gctttcatac cgccagagga
    12361 ttttgaatcg gcaatggaat atataaaata ttatagccaa gggatcactg cttttagaga
    12421 gtttgatcct gaagatgtta ttcatgactt gaacaaccta aaagaatggt gtgaaagtta
    12481 tggagtcgat gattccggat tggactactt gatcagtatt atggaaatcg aagaagagat
    12541 gccggataag cctgatccaa cagatatttt agaattgatc catgaagcta gaaatccaat
    12601 tgcctatatt tctagaggat atactgtatt ggaatactat aagccttata tatcagcgat
    12661 gaaccattta agacgtgtat taagggaaaa acgtgaatac cgctcagtgt tgaatgcaac
    12721 gaatagatta gaaaaagctg tttcagagct agatgcttat tatcgtgaac attacctgca
    12781 agcaggtggt atgccaagaa atacgaaagc aaatatcagt aggtatataa aaaaagctga
    12841 gtaggcggag ttagcccata aaatggcaaa aggtaacctg aaaggggcat tttgaatgaa
    12901 aagggatgtt tttgaattgt acgtattaga acgtctagaa gacggcatga tggcacaaga
    12961 gataattgat gaaattgatg atatgatcag cagatttggt caattggagt tgaatgatgg
    13021 aaacaaaaat tcggtagtgc taaaacaatt ttggttgaac aatttttcgt ataaggattt
    13081 tatcaaagaa aaggcaaata cattgtactg actaaattca cgaggagcag tgatgagagc
    13141 ttattgctaa gagcagttag gctctttttt gtttgtgtaa aattatttgt tttataatac
    13201 acatttatac acaaaaaata ttgacagata gtacacaaat gtggtaggat atatttgtaa
    13261 gcaagttaga ttaacaagtt aaaggaagag cagttatgaa aaaagaagaa ctaatagata
    13321 tatttcaaat tgttgaacga gcaaataata tgggaattat gttttttgat cgtatttctt
    13381 taaagatgga tttaagcgtt gcccaccaag aatttaatct gcgattaaaa gccttgttga
    13441 attctgatga tgttaatttc gctcatgatg ttgtaggtat acagaaccat atagatagag
    13501 aaaacaaacg tatgggtgat gaattcttac caagatattc aagtttatga tttggcgaaa
    13561 gattcaagag tatgtgtcat tattgatagg tactcttgaa ttggaaagga ggaatgaatt
    13621 taagacaaca acgattatga tcttaaaaca atcgtttatg taattggcaa tcttacgaat
    13681 aattttcatg caataatgac aataaaaaat gaaaaggggt aaaatcaaat gaaaaaattt
    13741 ttagtaggaa ttctattcac aatggggcta gtattattat caccttcagt ttcagcagaa
    13801 agctctattt ccatgtatcg tatgtataat cccaatagcg gagagcatct atacactaga
    13861 agcgttggag aaagagacaa tctgaaacgt tatggatggc attacgaagg tgtcgcatgg
    13921 caagctccaa caagtggtac tcctgtttat cgtttgtata atcggtataa cggtgaacat
    13981 ttttacacag ttgatgccgg agaaagagat aagatttcac acacaggatg gacgtacgaa
    14041 ggtgttgcgt tctattccta caacggagca aatagagttc ctgtaacacg tttatataac
    14101 aaatacgtga attggcatca ctacacgtta gatcaaaatg aaaagaacgt aattagtaaa
    14161 caaggttgga agattgaagg tacaggctgg tatgcaatgc caaacagcgt accaaatcct
    14221 acacctgctc caagcactga aaaagtgttg aacgttcctg ttgtatggca aggaaataca
    14281 atgttatgcg aaggagcttc tttattagaa ggtttacact acaaaggtgt agccacaaat
    14341 caaagcttaa ccagttttgt aaactatatg ccaaaagcta atgacaataa tccttatcat
    14401 ggttactcag gagagtggcg tcataacgtt aatggaacgt atcaaggcat gatggctgac
    14461 ccagtagtta aatgggcaaa aaacgttggc ggtaatgccg tcaatatcac tggttcagga
    14521 acgaatggaa tcaagaatga aattcgcaat ggtaatccgg ttgtaacttg gattacttat
    14581 aactatgcta cgccagagtt caaacaaatg ccttggggtc gtgcagtatg gaatggacat
    14641 gtagttctcg tggatggctt cagaaatggt gcataccatg ttgttgaccc tgtatttggc
    14701 gcaaaatgga tcaatgcaag cacgtatgag agagcattta atgtgactgg aatggcggtt
    14761 gctgtacgtt aattgataga attatcaact aaatgaaata agagcttaat tactgaaatg
    14821 gtaaataggc tcttattttt ttagaggaaa tttttgtaat acgaatctat aacaaaaaat
    14881 attgaatgta gtacacaaaa gtggtatatt aaaatcgtag agattatgcg aagaggtgat
    14941 tttgatgaac caaagaagaa gaaaaaggtt agaagaaata agcgagcgac tttccgagct
    15001 aaaggatgag ttagaagtaa ttcaagaaga agagtacaaa gaaaatatac ctaaaaatct
    15061 acagggatca gaaagatatg aaaaagcaga agaatccgtt gatcaacttg aagaagctgt
    15121 tgatggttta tccgacatat tagaattgat tgaagatata gctagctaga gatgattaat
    15181 gaagatttca ggagtgagaa taaaatgatt gatatttcac tgatagagaa gttacttttc
    15241 tcaaataagt acaccgcaaa agagctggct acaagatggc aaatatccga atcagctatt
    15301 aaagcctatc gtcaaaataa gggaagcgta ggatggcgtg attggaaagg gatcagcatt
    15361 ggaaaagcag aagacataat gaaacgtgaa ggacttcgat cctataccac ttctgaaaaa
    15421 gacaaataac ttaatgtttg aaaagataaa atatctgtct taaagctatg tcaaattcaa
    15481 atggtgaatg tttgctaaat ataatgcaaa aaaaggaagg gaaatattaa atgataagcg
    15541 aaaatttgtc ttatgagaat aataaacatc tagttaaaga aaataagaaa gtagaagatt
    15601 ataagaacat gtatatagaa atgaaaaaat caccactgtg tattcaatat aaaaatgata
    15661 attggaagca agtagtcgaa tttgtaaaac ctcatagtat acgaagagta cgaaggggga
    15721 agaaaaaaag tgaacttgta aatagcggaa gagcggtaat tagagttata accaaagatt
    15781 atccagttga atatggagat tatattttaa ggttaagtta tcatgctttt gttgtgttga
    15841 aagaagagca tttcaacgag tttttcaatt taaaaagtaa gtaatcatat ttttttgtag
    15901 acgtgtataa agttaactaa attgcgaggt gatgacatgg tagcaaaaaa gaatactcta
    15961 aaagaagctt ctgaaaaata tcaagatatg aaaagacgag gattaatacc gaaagacaaa
    16021 aaaggtatat ggagagaatt tgttaaggaa gtcagtagtg atcgaagtgg cggtgacttt
    16081 gttggacgat atacagcaaa aggctatagc aaacgattaa ggatcactgt tgaatatgga
    16141 acgaatgaat cagctacaga gctgaagcaa aggtacttag atacacgaag ggaagcttca
    16201 aaaaaatata atgatttcaa gagcagaggt ctgattccta aaaagaaaaa gggaatttgg
    16261 cgtgagtttt tcaaggaaga agatagcaat cggaataagg gagacttcat tggacgatac
    16321 acagcaaaag gctacactaa gaatttaggg atcaaggttg aatacggaac tggtgaaagt
    16381 tcatgggaat tgagaaatcg ctatttaaat acattgttag agaaatataa agcagaatta
    16441 ttggagttgt tcgatgttaa tgattttatc gagatggctg aaaagtatgg tcatgaagaa
    16501 ggttttatga tcaatgacat tatggttatt gataatttta cggatttatt agcacttgcg
    16561 aaaaaagcaa gggatttaag agaaacgcag atgtatctat tcagaaataa aaacggaaga
    16621 aaccggagaa ctgaatcgag tgagattgaa gattactacg atgcctttac acacgatctg
    16681 ggagcctttg tagacgagat aagtggtgta atgaacaaat atagggtcta gaataaaaaa
    16741 gccggagatg agcagataaa tgggaaacaa tagcatatac acacctgaaa tgcagaataa
    16801 aattaaaaga cttgtcggat tagggtggat gacaagagga aaattagatt cttttagtac
    16861 gatcaaatat cttttgatgg acatttaccg tcagaacaag caaacaggaa tacatttttt
    16921 gggctatgat aagccggaag atgcaccttt ctttgttcca agttcagcct atagagccag
    16981 tacctccggt agcatgtata acttggattt gtttgaggaa ggagcaaaga tgatcgaata
    17041 tgatttcaac gaagcctatt cgaggatcat gagaaattat cctttgccgt caaacactta
    17101 tttgaaagca aaatataata cagatcaagc tattttgaga gcgataaata gaccaaaagt
    17161 aagaccgtat gaaaatttaa gaacgtatat gttctttttg gttaattttg aaggaacagc
    17221 caaagaagga acatatgcta atccaaaatc acagtcgatg tttgcggatt atggcgaaaa
    17281 tcccaaaaca gctaatgagc ctatttggtt atcggagatt gagctaacac ttatccttga
    17341 ttattatgat attaaagaga tggaagtcct agatagccac acatttttga cgaaaaaagg
    17401 attactcaaa gaatattttg aaagaatcga gccattaaaa actgatcctg atacgttgaa
    17461 gctttataaa aatcttagaa cgaaagtatt tggtgctatc ggacaaaaat cattaagaga
    17521 aggagaaaca agcaccttcg ataaatacag tatgtataat cgagcgtatt cctccatggt
    17581 agcaggcgta tttagagatc gtatggttag atatgaacag aaatacgttc atagcgcata
    17641 tgggctatta aaaattcgta ctgatggatt gtatttcaga tccgaagtac cagagtttga
    17701 agagctgtat aaacttggag tagttaagaa aaaagttcat acaatatcca aagaagaagt
    17761 cgaaaaagct ttagaattta aaaacaaagg atttgaaaat aagggctcct ttgggaaatc
    17821 taaagatgaa gttaagggtg ttgtgataga cgatgatttc gagtttgatt ttgactaaat
    17881 actctttgga aaagatggta ggaggtaaat atgaagaaga aaattgtgga aggaaatgta
    17941 ggtgttatta ttctaaaaga ttcaactgac tatgctaaat caatggcact aaaagacgaa
    18001 aaagttgcac gcatggataa agatttagtt gaatttctga tggatgatac agaaagatta
    18061 aaaggggatc agtttttgag tttaatgaaa gacaaaggct taaacacata tggtttattt
    18121 gattataaga aattagctat agagtgggta aaagaaggag aaatattttt tattgaagaa
    18181 tgggaaggta ctgagaactt agttcttcat cagtatcttt tgaaagctta atctaagtaa
    18241 aacagttctg actagttatg cttgtcagaa tttcaaagga agttttccat aaccatcatc
    18301 attcaaaaat aaaggatttg aaaataaggg ttcctttggg aaatctaaag atgaagttaa
    18361 gggtgttgtg atagacgatg atttcaagtt tgattttgac tagatttttt tgaaaggttt
    18421 aagagtaaac aaggtaagaa ctaagatcat gaagattgag agtaaagaag atagaggacg
    18481 aatgtatgag aacacactat atttatttag aagataaaat tgtagatgaa attccggcaa
    18541 aaagtcgaga acaagctaga aaattattta ttgaaaaata caaagagatc aatggagata
    18601 gtgaagttcc aaaagtggtc gtaacagtta gaaaaaggca aacaaaaaag agaagtacgt
    18661 ttcatgtcag ccctgatata tataatctta tagagcgaca tagaagtgaa gataatattt
    18721 ctatctctca atatattgct acagttattg atgaattgat ttcaggaaag tatgactatg
    18781 cttttgattt tgagggtgaa aaggattaca aaagtgttgc tgtctttcaa tctgattttg
    18841 agaagctaaa aaactatgct ggcaggagaa atgttaattt tgcaagacta ctagaagagt
    18901 tctactccga agagttggga gtaggaaatg aatacaagag agaaacttat gcgaaattgt
    18961 ctcataagtt atcccaagcg cttaaagaag tgacagaagt taaagcggaa ctagatgcta
    19021 aacttgaaga agtgaaaaaa tatcaaacag agaggaagaa caacatgaag aatcaagtgg
    19081 aatttgacga acagaaagaa tttgaaaaag caagaaaacg ctataacaaa attatgaatg
    19141 aacttagcta tgaacactta acagttcatg aataattgag tgagggtaca gaaaaatata
    19201 cgatcgcaga tatggttaat gaagtggaaa ctcttttagc ttctttcttt gaaaatggtc
    19261 acgtaaacga tgaatggcga aaagaagaac ctaaaagggt taacgcttat attgctcgat
    19321 taaaaaggtt tattgataaa tataaggaga atagtgacaa attttctaaa gataaataag
    19381 gagagagtgt gattacattt gccagaaatc gaaggaatat tttcgccaaa aataggtaaa
    19441 aaacagttag atttcagcag ttttccgagc gtaaatagag attataatct aggtgatgag
    19501 gtacagatac taaatattga tttcaaagaa ctagacttcg ctgttgctgt ttccaacaaa
    19561 cccaaggcat tggaacgagt gaaaaaaata taccaagaga atgatcaaaa tatcaaaaat
    19621 atgatcaaaa agtatagacg agataattca aaagttgaag acgttttatt caacggtacg
    19681 gcaaaacatg atttgaatgt ttatatggca ttaggagtgg ctttgtactg taattcaatt
    19741 aatcagacag atattttggt taatatcgtg aggaatagct atcaaaaaat attaaaaata
    19801 agtagcgaaa caaaccctta ttatgcaatg aaatatcttg gggataattt attaaatgat
    19861 aaaaaattga tgcacaacac tgaagagctg ttatctaatt tggctgcgat taactttcta
    19921 ttcaaaacag gttctgaaga ttattttata gataacgtac taaaaggtgc ttacggaaca
    19981 gcattttatc cgctttacaa tatgaaacag cataaagccg aacaaaagga taacaaactt
    20041 gaaattaaaa agatgaaaga actattaggt ataggatttg taaaacccaa ttcaaaagtc
    20101 actattgctt cgttagcaac tactaacaat gatgggaaaa tagtaaatgg gtttgacgat
    20161 tcagatatag atatattgtc aaaatcatat ttttctttgt atggagattt tgcagatttg
    20221 atgggtattg atagttactt agtcaataat tttgagatca agggtagcga gctgaatgat
    20281 attgtgtatt tcgttttaat ctctctatat gcaattgatc agaataccga gtaccgacaa
    20341 attgactatt tgctagtttt ttatagtttg ttatatcagt tcgttttaac tcggttgttt
    20401 aaaaatgcta aggaagaatt gatcctgaat tctgattatg aaatagcaaa acaaattgca
    20461 agtattagaa aagaaaacaa atctgaagta atcaaattaa gaaaagaatt aagtattgcg
    20521 agtaaagaaa acggtcggtt agaatcttta gtcaaatcgg caaaaaaaga agaggaactt
    20581 ttgaagcaag agattaaaga attaaaagga atcagaaaag aacttgaaca gctgaggttt
    20641 gaaaataaag tattaaagca attgcaggag caagaacata gacaatcatt aaatggtaaa
    20701 actgaagagc cttcactgtc tgaaatgata gcttatctaa aacataaaaa cattctttta
    20761 gtgggaggac atgtaaattt tcaaaataaa ttaactgaac tattaccaaa tattaaaatg
    20821 tttgatgtaa gcgaaaataa tggaaagata cttaaaaatt tgaatacgtg tgatgcgatt
    20881 tttctatata cagattactt gaatcatg
//
LOCUS       DAZKOB010000044        19760 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000044 DAZKOB010000000
VERSION     DAZKOB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.044"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(787..1740)
                     /locus_tag="ACG8M6_001547"
     CDS             complement(787..1740)
                     /locus_tag="ACG8M6_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0457223.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1763..2518)
                     /locus_tag="ACG8M6_001548"
     CDS             complement(1763..2518)
                     /locus_tag="ACG8M6_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457224.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2515..3474)
                     /locus_tag="ACG8M6_001549"
     CDS             complement(2515..3474)
                     /locus_tag="ACG8M6_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0457225.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3467..4417)
                     /locus_tag="ACG8M6_001550"
     CDS             complement(3467..4417)
                     /locus_tag="ACG8M6_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0457226.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4567..5019
                     /locus_tag="ACG8M6_001551"
     CDS             4567..5019
                     /locus_tag="ACG8M6_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0457227.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5108..7375)
                     /gene="gshAB"
                     /locus_tag="ACG8M6_001552"
     CDS             complement(5108..7375)
                     /gene="gshAB"
                     /locus_tag="ACG8M6_001552"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0457228.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7562..8560)
                     /gene="ftsY"
                     /locus_tag="ACG8M6_001553"
     CDS             complement(7562..8560)
                     /gene="ftsY"
                     /locus_tag="ACG8M6_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0457229.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8571..9380)
                     /locus_tag="ACG8M6_001554"
     CDS             complement(8571..9380)
                     /locus_tag="ACG8M6_001554"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0457230.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9389..12970)
                     /gene="smc"
                     /locus_tag="ACG8M6_001555"
     CDS             complement(9389..12970)
                     /gene="smc"
                     /locus_tag="ACG8M6_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0457231.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12990..13676)
                     /gene="rnc"
                     /locus_tag="ACG8M6_001556"
     CDS             complement(12990..13676)
                     /gene="rnc"
                     /locus_tag="ACG8M6_001556"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0457232.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14024..15805)
                     /locus_tag="ACG8M6_001557"
     CDS             complement(14024..15805)
                     /locus_tag="ACG8M6_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0457233.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15831..16745)
                     /locus_tag="ACG8M6_001558"
     CDS             complement(15831..16745)
                     /locus_tag="ACG8M6_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0457234.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16772..17734)
                     /gene="opp4B"
                     /locus_tag="ACG8M6_001559"
     CDS             complement(16772..17734)
                     /gene="opp4B"
                     /locus_tag="ACG8M6_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0457235.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17734..18678)
                     /locus_tag="ACG8M6_001560"
     CDS             complement(17734..18678)
                     /locus_tag="ACG8M6_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457236.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18679..19683)
                     /locus_tag="ACG8M6_001561"
     CDS             complement(18679..19683)
                     /locus_tag="ACG8M6_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457237.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5462 a         4257 c         3519 g         6522 t
ORIGIN      
        1 gattgatatt aagcgagtat aaaatcaaag cagaagagag ttatttctat caattactaa
       61 aacggtggag taagcttccc gccgattttt atcgaaaaat gcagatcatc aactatcaac
      121 cgcttgctga gacacatgcc taaatagagg ttgtgaaatc agtgctttga gctaataccg
      181 aaggtcagct gattgaacat tgtttagaag agattgtgac aactgcgatt tgctttgagt
      241 aataagaacg ctggacagaa aatagcttct catattttct agtctagtgg gcttattgcc
      301 gagaagcagc agatggaaca tcgtttagaa gagattgtga caactgcgat ttgttttgag
      361 taataagaac gctggacaga aaatagcttc tcatattttc tagtctagtg gacttattgc
      421 cgagaagcag cagatggaac atcgtttaaa gaagagatcg gtacaaaata aaaatatcta
      481 aatttaagtt ttatctaagt ttcgctttgt acctttgatt caaatcaaag gaggcgatct
      541 tgatgctgaa aaaatggatc acactatttt ttgccactcc ccattctaga atcaacccgt
      601 atgatgtaga taatgaattc acgttgaaaa aagatttctc aaataaataa aaaccatccg
      661 taaaatcatc aatacgattt tacggatggt ttttgataac ttctttgagg ataggctctt
      721 gctatttaag aatgtaagaa acattattgt tacatagtat gagagtttaa tcctctgtta
      781 tttatgctat ttaaatgctt gattcacatc ttcgatcatc agtttcattg attctaaacc
      841 gcccccgctt aaataccaga catctggttc aagggagatg atctgctgat ttttaccagc
      901 attcgtttgt gctaccagtt cattggcaga aatatcatct ttgctgtcgt ctccaccaat
      961 agcttttgtt ctgtctacta caaataagat atccggattt ttttccagca catattcata
     1021 agaaacactt tgaccgtgag tggatgcttc gatttgatca tctgcttgtt caaagccaaa
     1081 taaatcatgg atgaaaccga atctagaacc agatccgtag gctgataatt gcccttcatt
     1141 cacaagtgtg actagtgctt ttttgtcagt agcttctgct ttctttttcg tctgatcgat
     1201 tgcactagat aattcctcta attgagtttt agcttcttct tgcttaccaa agatctcccc
     1261 taaagcagtg acattttgct ggaaagattc ccatggattt ttcgcatcta aagaaaggaa
     1321 aatggtcgga gcaatttgac ttagatcttt ttgaaaatct ctttgtcggc cagaaatgat
     1381 gattagatct ggctgaagct gattaatttt ttctaagtct ggttctttta ttccacctgc
     1441 tgattctact tttttatagg atgaaagata ctcaggaagg ctgcttgtag ctgctccaac
     1501 taccttatct ccaacaccaa gagcatccat cgtatctaaa gaaccattgt cgaatacgac
     1561 gactttttct ggattatttg gcacctcgat ttggtctcca ttactatctg ttatcgtcaa
     1621 tgtagtttca gtttgtgtgc tgcttttttt tgtgtctgcc tgactggtgt tgttgctatt
     1681 tccacatgct gctaaaccaa atgatgctag aatcaagata cctactacta attttttcat
     1741 ctcttctctc ctaatgttag ttttagaaat aaagacagaa tctgcggcct tcaatctcac
     1801 aaatccgtat gtccatttcg tataattcat ccaatacatc tttttttatc atttcttggg
     1861 tcgttccttg cgtaaataac tgtccatctt tcattgcaac aatttcgtct gcataacttg
     1921 cagcaaagtt gatatcatga agaacaataa caattgtttt cccgatttct tgtactaaat
     1981 tttttaaaat ctgcatgatt tgaaccgcaa aattcatatc tagattgttc aaaggttcat
     2041 ccagtaaaat ataatccgtg tcttgcgcta gcaccattgc aatatacgca cgttggagtt
     2101 gaccgcctga aagagtgtca atcgattgat ccgctatttc ttctaaccca atgtgttcca
     2161 gtgattcctg aacgatttcc tcgtcttttg tcgtcaattt tcctttcgta taaggaaatc
     2221 gtccaaattc tactaattct ttcacggtga tttggacatt tatttcattc gattgtcgta
     2281 ggatcgataa ttttttggct aattcattgg atttccaagc ctttacttcg ttatggtcta
     2341 aatagatttc acctgtatct tttggtatca aacgactgat catcgacaac accgttgact
     2401 ttccagcacc atttggaccg ataaaagcag tcaacttctt ttcaggaatc gttaactgta
     2461 catctttaac cactgatttt ccatggtaca gtttattgac cttttgtaaa tcaatcatag
     2521 gatactcttc ctttcgcgaa tcaattttgt gacaaaataa attcctccga caaattcaac
     2581 gacgacactc actgttgtat tccagccgaa aatctgctct actgcgagct gtcctcctgt
     2641 cagaaacagc atagctacca gaaagcttcc gataaatagc tgaccatgac gataagtaga
     2701 aaagacacga taactgatat ttgcgacaat aaaccctaaa aaagtaactg gacccacaag
     2761 agccgttgat gtccctgtca aaagactgac acaacataaa atcaaaaatt caaaacgatc
     2821 tgtgtctatc ccgaggttta ctgcttgttc atttccgaga tggaaaacgt ctagcacatg
     2881 tcgatatcga aataacaggt accagcagcc gataatgatc aaagaagcag gcaaaagata
     2941 ctgactctgg atattgccaa agcttgcaaa taatctgcct tgaagcaaat catattcatt
     3001 cggatccatg atgacttgta aaaaggtact gacactacca aaaagcgtac ctaaaatcat
     3061 ccctgacatc agtaataaaa aaagattcgc tctcattttt ttcaaaagaa agaaaaataa
     3121 caatagactc agcccgacca tcagcccaac gttcaataag aaaaaaactg tacctgtctg
     3181 ctgaagcatc gtcactcctc cgataaagaa gaaaaggacc gtttggacaa ggacatataa
     3241 cgactcaagt cccaatactg aaggagtcaa aaactggtta tgcgtaattg tctgaaagct
     3301 gattgtggca ctgctgacag ctagcgagac aaggataaat gcaaaaattt tcttactcct
     3361 caatgcacat gcaaaatgcc agtttccata tgtatggatc gtcaagtaca ttcctgtaac
     3421 aaataaaaca agaacaagca taccaataaa aatagatggc ttttttttaa gcatatcgtc
     3481 gtccccttat cagaagataa aggaagatag ccccaccgat gattccaacg atcacactga
     3541 ctggtatttc ataaggaaaa atcaacacgc gagcgacaag atcgcaaaat actaagaaca
     3601 aactacctgc taaagcagtt acgccaattg ttttgtgcac ttgatctcca tatattctag
     3661 agacaagatt cggaatgatc actccgataa aaggaatgct tcctaccatg atcaacatga
     3721 ctgcactagc caatgatact aagccaatgc ctaaaaactg catcttctgg taatctattc
     3781 ccagatttac tgcaaacgat tcgcccagac caatgatcgt aaatcggtaa gcaaatagcc
     3841 aaaccaagag caacaatgga acaacagcat acaataactc ataatttcct cgtatcacag
     3901 tcgcaaaatt cccttgaagc catgaagtca tattttgtac caactggaat tgataagcaa
     3961 aaaaagtagc aactgaacca atgatattcc ctaacatgac tccaatcaac ggaagcatca
     4021 attgattttt ctggaggaac aatcgcgtaa gggataaaaa aagcaatgtt cctgcaaaag
     4081 caaagcaaaa tgctgtcaag gatcggacaa tcaaggatgc accaggtagg aaaatcatag
     4141 ccactaaaat acctaaacgc gcactatcca tcgtgcccgc agtcgttggt gaaacaaatt
     4201 tattttgggt caaatgttgc atgatcaacc cacaaatact actggtcgct cccacaagga
     4261 tcaaacaaat cgttcgtggc aaacgagtca ctagcaagat ttgtaagctt tgcccatcta
     4321 actggagcag ttctgacaat tgtacattcg atactccgat gaacaatgaa atactactta
     4381 aaataactaa taatgctact agtcgtccgt agcgcatcca atccaccttt ctaattgaga
     4441 atgattttca ttcttatttt aagtgaatcg ttgaacaaaa gcaaccgctt tttcatatgt
     4501 tttaggtatt attgggaata tgctataatc gtaagaaaga caaagagaag aaggagggta
     4561 ttttttatgg cacaagatga tgtaaaaagc catttggata agggacttta tggtacacct
     4621 ttagtgaacc ctgaagaaca gcataaatat ttagggacat tcagggaacg ctgctatctt
     4681 agtatgactg ttgctgaaat gatccaacca aaaaacaaag aaaacttttt aaaagaactc
     4741 aaaaaacatc cagatgcttc catactcttg aatggggctc tacccctttc tgtccaaaac
     4801 caatatatcc agctagcaac gaaaaacaat agccgtttca ctgtagtcaa cgattacgtc
     4861 actgcaaatc ctgaggaatt cggattgctc ttaacagcaa aagaagcagt aaatgaacct
     4921 gtgattgata tcgaaaaaaa atacccagaa gaaaactctt cgccagctga aaaagaaaaa
     4981 ccaaaaaaga aaaaaagctt ctgggatcgt ttattttaaa acgaaaaaac acataaattt
     5041 ttacatgcaa aaaaagcttt tgatcagatt gacatcaaat aggacaatct gtcaaaagct
     5101 ttttctttta aaatacttca ggatataaca atttcaatac attcatcgtc aatcttctgc
     5161 cttttcctgc aaaaggataa acatgcatat gcatggctgg attgaaattt gcttcgatga
     5221 tcccatatgc ttttttatca gttgtaggat cgatttcttt atctggtata atcaaatcga
     5281 taccactaat cttagctcct agtgcttcta ccgcagaaac ggcgattttt ttgtaagctt
     5341 cagaaaattc atcagtcata tcgatcgaat caccacctgt actgatattt gagttctccc
     5401 ttagataaac aatttgattg gcttggggaa ctgattcgat cgtcaaccct tgttctttca
     5461 acatcaattg ttctaattca cctaattgga tcaattctaa aggtgcacga tgattcgttc
     5521 cacgcaaagg gtcactattt ttttcttcaa caagttcttt cactgttcgg atactgtcac
     5581 caataacatt agcagggact cgcaacatga tggcttggac ttctccgtcg atcacaaaga
     5641 aacgatattc cgttcccggc ataaattctt ctactaaaac cgaactgtct tcacgaaaag
     5701 caatggctaa acctgcttga taatcttcca gactagctcc ttccttgaaa atcgtgatac
     5761 ctaacccgta attggttgat ttaggtttga tcacaaatgc ctgctcggca aatctcggat
     5821 aagcttttac cgcttcttcc atggatgaaa attctgcgcc tcctggtacc cggaacccag
     5881 cttcttttaa gacttttttc gttactgttt tgttttccat gataagtggg acgatatagc
     5941 tgtccttact tgtcatgttt gcatttttca catattctac atggtcttgg aattgcagac
     6001 gcaaaaattg atccgactca tctaatactt tgacttggac acctttttgg atggcatcga
     6061 acataaatat ctgagtagaa agttccattt cgcgaaaacc ggccaactga tagggacgtt
     6121 catggctttc tttataatat tgatgtccca atgccacagc aacttcttgc tgactcgata
     6181 gctgtgcttc ttgatagagt ttcgctgcta acgtttgttc aggatgatcc atttgagtca
     6241 atgcttcctc tactaaataa agagaatcca accaatccaa ttcaaccagc atttgcttca
     6301 ttccttcaag aatttctcta ccttcttgat gaaattctgt ttgatctgat ggttgttcta
     6361 gtgctacttt gttatttcga gtcgtacctt ctgctaccca ttggtcacag tcttcttttt
     6421 cttctgtcca taacatgtac atcaggaata attgtaagaa ccgaacttgc tctggattga
     6481 tacccaatct tgcataagga ttcaaatcga tattccgcaa ttcaatataa cgaacacctg
     6541 ttgttgctaa atctgcgact ttctttccgc ctcggaaacg aagcggtgta taaaattctt
     6601 tctcctccga aagtttccct tcttcgatca ttcgctcgat atctgctaaa tactgtttca
     6661 tagaagcata ggaaactttc acatttggat gattcgtata gcccagcgca ctgttgcgaa
     6721 tactgcgaac aggttcttgc ggatgtgatt catctaaaaa gtagttcttc tcactcattg
     6781 gtgaagcacc gaataaataa gtgatcatcc agcgatagcg cataaagttt ctcgctgttt
     6841 tcaaataaag ttctgttttg aattcggaaa aatcttgaaa ttcttcctga tggctgaaca
     6901 atgttcttag caactcatcg ccaaattcaa aattgaaatg aataccgctt accatctgtt
     6961 ttcgcttccc gtattctttt gctaaataac gtcgatacaa gacatcttcg aaatttttta
     7021 attttgcaat aatgatctct tcgtcttttt ccggtaaggc aggtggcata cttaatggcc
     7081 agatcatctc ttctttcggt atagaacgag cagtcacatc ataaacagca gcgagatact
     7141 gaaataattc gggaatagaa tcggtaacag gggtgatcat ctctgtttgt gtttcgctaa
     7201 aatctgtttg gatatagggg tgataggatc gatcgccaaa gattgctgga tgatcggttt
     7261 ttgctagatt tcctgcaaga tcaacccgct gtccttcccg ttctatacca aatcgagctt
     7321 ggtcgataaa agggcgcgca ttgacatgca ataataattg cttaaaattc atcattaagt
     7381 gacctccaat tcatatgaat ctaattataa tcaaaaagtc taagaaaaag aacgaaattg
     7441 tttgagaacc gcttacaaat aagtataaaa atgaaggcat caaaaacaca gctatgttaa
     7501 aaacaatcat tcaaacaagg agctgcaact tttgtcacag ctcctgcttt ttagacatgg
     7561 gttattcttc ttttagtaat cctttgaaca atccaacgac aaaatcattt ggatcaaacg
     7621 gctccaagtc atcgattcct tcaccaagac cgaccaattt cactggtaaa tgcaattcat
     7681 tacggatagc aagtacgata ccgccctttg ctgtaccatc taattttgtc aaaacaagac
     7741 cggtcacatc tgttgtttct ttgaattgtt ttgcttgaac catcgcgttt tgaccagtgg
     7801 ttgcatctaa tacaagaagt gtttcttggg gtgcttctgg taattcccgc tggattactc
     7861 gttttatttt ttctaattcg ttcatcaagt tcactttatt ttgcagtcgg cctgcagtat
     7921 caactagtaa aacatctgcc tgctctgtct ttgctctttc caaagcatcg aatacgacag
     7981 ctgctggatc gcctcctgca ttgccacgaa caacttcaac accagcgcgt tcgccccaga
     8041 caactaactg gtcgattgct ccagcgcgga aggtatctgc tgctgccatc aatacttttt
     8101 taccttcgtt tttgaactga tgggcaagtt taccaatact tgttgttttc cctacaccat
     8161 taacaccgac aaacaaaata actgttagtc cgtcttcttg gatatttagt tcatttactt
     8221 cattgattcc ttccgcttca tagagatcca caagtttctc gatgatcgca ttttggactt
     8281 ctgccgattt tttcgcattt ctgagtttca cttcttgacg taatgcttcg gtgattcgta
     8341 tcgctgtttc aaaacctaca tctgcaccga taagtgtttc ttctaattct tcaaaaaagt
     8401 cttcatcgac tgttcgaaaa ttggcaaaca actcattcat ccgttcacca aatgttttac
     8461 gcgtcttttc taaacccttg tcgtacttct cctgtacttc aacttcttct gttttctctt
     8521 cgccagtaaa agcacgcttg attttatcaa aaaatcccat ttgactcccc ctactctaat
     8581 acaaattttt ggatcacttt ggctacgcca ttttcttcat tgctggctgt cacgaaatct
     8641 gctgcttctt tgacgaaagg cacagcattc tccatcgcta cgcctagtcc agcatattgg
     8701 atcatcggta aatcattttc ctcatcaccg atcgccatga tttcttcttg tttcaaacca
     8761 agatcttttg ccaataaaga aataccataa gcttttgtaa caccttttgg cataaattcc
     8821 aaaagcatac ttcttgtttt gatgatctca tacatttcat ggaattctgc tggtatttct
     8881 ttgattttag gatctaattc ttcttggtca taagcgatta ctgccttatt aagcacaaat
     8941 tcatctgtaa tatcgcttat atccgctggt tggaattgca gcagcttatt caacacattg
     9001 tacaaggatt gcttccttgg ggcactaggc agctgtaaaa ccacattgtc agacaaaaca
     9061 tctagtggca gatccaattg ctgagccagt ttgtataagc gttggatatc tgatacttcc
     9121 atcactttct tttccatgat ttcaccagta tcattttttt gtaccaaacc gccgttgaaa
     9181 gtgatgctgt agtcaccttc atctaccagt cctaattctt gcagataatg cgccatcgca
     9241 gctaaggggc gtcctgtaca tagaacgatc ttgacccctt gctgttttgc ttgtgctaat
     9301 gcttgtttat tctcatctga aatcagcttt tgttcattta ataatgtacc gtccaaatcg
     9361 attgctacta atttgatcat tttcgttcct acactttcgt ttcgttgatt ttcgcctctt
     9421 cattgatatc ttccatccgt acagaaacga tttttgatac accggattcc tgcatcgtca
     9481 ctccgtacag tacatctgct gcgaccatcg ttcctttgcg gtgtgtgact acgataaatt
     9541 gtgtgtcatc ttggaaatcg ctcaagtaac gaccaaaacg tttcacgttg gcttcatcta
     9601 acgctgcttc tacctcatct aagatacaaa acggaacagg acagacacgg ataatggaaa
     9661 agagcaacgc gatggctgta agtgctcgtt ctcctccaga aagcaaactt agactttgca
     9721 gttttttgcc aggaggttgt acttcaatct caatacctgt tttcagcaaa tcagttggtt
     9781 ccgtcagtac taattcagct ttaccgccgc caaacatatt tggaaagaca attttgaatt
     9841 cttgacggat agcttcaaat acttctttga atcttgtccg cacttcatca tccatctcat
     9901 ccatcgtttc aaacaattgg tttttcgctg ctaacagatc atctcgctgt gttgctaaga
     9961 atgtatgtct ttcactgact tgttcgtatt gttcgattga atttaagttt actggtccca
    10021 gtttttcaat ctgttctttt aaggatgaaa cctttgttct actgtcttcg atatcggttg
    10081 tttcttgata gtcttgggaa gctttctcaa atgtcagctg atattctgac tgcagatagg
    10141 tcaaatgatt atctaaaatc atttcagctc ggtctttttg gacttctaat ttggattgtt
    10201 ccgacagtcg tgctttttgc tcacgattac gctctgccaa gactgcttca agcttatcga
    10261 tctccttttg ccttctatct cgctgctctt tggcttttgc caactcggct tttaatgttt
    10321 ctcgctgagc agataattcg ttaatttgtt tttccaaact ttcttctgtg acttcatgat
    10381 cagaaaagtc tgctgtcaat gtcgccaact gcttttctat cgcttcttga cgttcggttg
    10441 cctctgcttt ttggactcgg gcaccgcgga gctggatctg cagatgatta aactgctctt
    10501 tcaagacagc aagatctgct tgttcttgtg ctttttgact ttgtacttgt gctcttctgg
    10561 cttccatctg gtcgctttct tgacttaacg atttgatttc ttcgtcgatt ttttgtcgtt
    10621 gcgattcaag atctgtttgt ttgtctttca attcgttcgt ttgcttttcg taatcttcga
    10681 taaactgctg cagttcacgt gtctcaaaag ttgagatttg cttttctttt tcaaaacgtt
    10741 caagttcatt tatgatgttt tgtagctgat ttgtcgcttc ttgttcttca aaacgtaatt
    10801 gttcgcctcg tgtacgcaaa acttcttgtt cttcggctaa acgagctgtt tcttgttgca
    10861 gctcctgaac ttttttttct tgtgcttgga gttgtttatc tgcttcttca aattcactag
    10921 tcaactgttt caattcttga ttttgtacaa acaaactgcc ttggtttcct cgtttattcg
    10981 ctccacctgt catggatcct ccggcattca tcacatcacc ttctaaggaa accactcgat
    11041 attgatagcg gatcgtttgc gcgattgcat tggcactagt taagtctttg gctaaaagga
    11101 tcgttcctag caggttttga accacagttt ggatttgatc gggataactg acttgttcac
    11161 tggcaatccc tataaaccct tctacagctg ctgcttgtgt caatatatga gccggtaatt
    11221 gtcttggttt gattgtcgtc agtggaagga atgttgctct tcctcctctt tgctgtttca
    11281 aatacgtgat ggcttgccga gcatcttttt cattttcaac gatgacatgc tgggctgccc
    11341 cacctaaagc tgcttcgata gctaacgtga aatctgcagg aacatcgatc agttcagcca
    11401 ctgctccgac aatacccgaa agctgctgtt tatgctgcaa gaccaatcga accccttggt
    11461 aaaaaccaaa atagttttct tggatttctt gaaggctttt cttacgagca cgtagctgct
    11521 ggacttgatt cattaattga tacattttag gctgttcttt tgctaatgct tcttggaact
    11581 ttttcccgtt ttgttggatc atttctaact tctcttttgt ttcagttaaa gatgatttta
    11641 gctgcgcctg cttttgtgtc aattcttctt tttgtaagat caatcggtct actgatgctt
    11701 ctacttccga ttgcttggct aacgtctgct tgctttttgc tgtttcttgt atatattgac
    11761 gttctagata cttcagttcg tttcctacag ctgcttgttc ttgcatcaaa tcaacatatt
    11821 gatctcttaa ttctgccaat aattctttcg ttgatttttg gtatttttct aattcttgct
    11881 gtgtttttat gagatttgct tcagcttttt ggatctctgt ttctttttct gctgctgctt
    11941 tcatcaagct ttcttgaagc ttttcaaaat gtttaacctt tttttgtgct tctgctaatg
    12001 aagtttgata ttcttgcgag cttttttgtg tatgctttgt ccgctcctgc aaaacatctt
    12061 tttgtccttc tgtctgcttc agtttttccg aaagatctaa taatacttgc tgattttttt
    12121 cgatcagacg atcggcttgc gcattttctt tacgctgctt cgccaaaatg gattcttgtt
    12181 cttggatcga ctcagatagt ttgcctaatt ctagattgaa tttcgcaagc tgtgcttgtt
    12241 tgttgtccca atcttttttt gctgtcttga tttcagcaac catcaaagag acatcggttt
    12301 gagtcagtgt ttcttttagc cgtaaaaact cctttgctgc ctcactctgg gcggccaaag
    12361 gagttaactg ttcttccaat tcgtggatga tgtcttggac gcggctgagg ttgtcttctg
    12421 tttcaaaaag cttctgttct gctttctttt tccgctgctt gtatttcaag acaccagctg
    12481 cttcttcaaa gatcccacga cggtcttccg gtttactact gaaaatcgct tctacttttc
    12541 cttgagaaat gattgagaac gattcttttc ctaatccaga atctaagaac aattcttgga
    12601 tatcttttaa tctgcagctt tgtttattga tgaaaaactc gctttcccca gtccgtcgat
    12661 aacggcgggt tacactgatc tcattgtact ccaatggcag ataatgatca ctattgtcta
    12721 aaatcactgt gacttctgca atattcagct gcttacgggt atcggaaccg gcaaagatga
    12781 tatccggcat ttttccgcct cgcagacttt tagcagattg ttctcctaat acccagcgga
    12841 tagcctctgt aatattgctt tttccgctgc catttggtcc aaccactgct gtgacgctat
    12901 tttcaaaatc aatgactgtc ttgtctgcaa atgatttaaa accggctatt tcaattcgtt
    12961 tcaaatacaa agctttccct cacttacttt tacagtgctg ccaatgcatt ttccgccgca
    13021 gcttgttctg ctaatttttt cgatttccct tgtccagtgc caatcaattg atcatctaca
    13081 taaacttcga tccaaaatac tcgttcatga gctggacctt cttcattgat caatcggtat
    13141 tcgattgata catcacctga tttttgcaag acttcttgta gttttgtttt atgatccatc
    13201 tcatgtgaaa aagcacctgc tttgacttta ggaaaaatca ctttttcgat gaatgcatgg
    13261 gtcgtgtcga atccctggtc aagatataac gcgcctaaaa aagcttcgaa taggtcgcaa
    13321 agcaatgctg gtcgcgtacg tcctcctgaa ttctcttctc ccttgcccaa caaaacatat
    13381 tgatcaaagt gacattcttt ggcaaatttc gacaagctgt cttcgcgaac gatcgcagct
    13441 cgtgtttttg tcaattttcc ttctggcaaa tgaggatata aacggaataa atactgagaa
    13501 accatcagtt ctaaaacggc atctcctaaa aattcaagtc gctcattatc cgataattgc
    13561 agattgcgat gctcattcac ataggatgaa tgggtaaaag cttgttcaag caaatttaga
    13621 tcgtgaaaaa cgatgttata gttttctttt agttcttttg tcagctggtt gtccattttc
    13681 cacattcctt ttatggatat tttttcgata cttccttatt atactggttt tttgtcaaat
    13741 ttgaaatggt tcggtcaaaa ggtaagtaaa gctttaataa tcctagtgat tgacgagaag
    13801 ttgtgaaaaa ttcgtttagt ttcgagtatg aagaaaaaat tttgtgaaaa tggctttcca
    13861 tatttttgca taaatttgac ttaataccga gaaactggac tttgaacacc gtttattgga
    13921 gaagtcatga aatcagcgcc ttgacctgaa tattagagaa ataaacagaa aaatatcaaa
    13981 aaaccgaaaa aggacatcgc cctttttcgg tcatttttag tgattatgaa cgtgaatctg
    14041 ctgtaacaga aacatttgcc catgggtttg gtacatcata agaccaatcc cagtctttga
    14101 cacggtcgtt gactggcaat acttcattac ggaacaacgt tggaatcgca aatgcttctt
    14161 caaatgcata ttgttgccat ttttgatatg cttctttttg gtactcttca tcaaatgctt
    14221 tgcttgaact gatatcgttg atcaattttg tattttcttc tgattcataa cgagtgtagt
    14281 tgaacgcagc atttggtccc catagtccac ttggatttgg atcagttcct gtattccaag
    14341 cagcttggta aacatcaatt tcaggatcat cgttttctaa tttatcatag aaagaattga
    14401 attcgatcag acgacctgtt gtgtattgga catccaaacc aatttctttc cattgttgga
    14461 tatagtaatc tgccaatggt tgcgctgttt ccccaccgga catcgaagca aagttgattg
    14521 ttagtttatt tccatctttg tcttctctta aaccatcgtc atccacatct ttgtagccag
    14581 catcatctaa tagttttttc gctttgtcta gatcttgtgt gaatcctttc atctcatcat
    14641 catggtatga ctggaatact ggaggaatca atgaagtcgc attgcttctc aaacctgcat
    14701 agaaacgttc acctactgca ttattatcta atgcatagcc cattgcttga cgtaatgatt
    14761 tatcagccat ttttgaatct ggattgtatt ccacactctt tgtatctgca ttccatttac
    14821 ctaacttgaa tcctagataa gtataagaag tttgttcacg gcctaagttt gtatagccag
    14881 caatttcttt ataggtatcg taagtatctg ttggcatttg gaaaatcata tcatataatt
    14941 tagagttcaa tgcttcagtt gcacttgcca tcggtacaga tttcatcgtg atctttttca
    15001 atttaggcgt accattccag tagtgctcgt ttggcaagta ttctactgat tctcctgcaa
    15061 cgatattact catgtaataa gggccaaaag tcactggatt tttacgtact tgatcgcttg
    15121 attccatatc tttgattgct acaccttcta acgtatgttt tggtgctgca tatgaccaga
    15181 taccaccacc cgattgaagc atgcttgggt ttacttcttt atattcgatt tcaactgttt
    15241 tgtcgtctat ttttttgatt ccggagattg tgtcggcact tccatcatga tattcttcca
    15301 tacctacgat gttacggaaa gtgtcgtcat aacggatacc agtgtaatct ttattcccaa
    15361 tgatttcgta agggaaaatc acatcgtccg ccgttacatc ttcaccatct gaccatttga
    15421 cgttgtcttt tactttgatt gtcgcttttt tcgtatcttc atctagatca agcgttgcag
    15481 ctccgtcgtc tgtgattctg aaatcgtcat cgtttgcaaa tagcggttca tgagaaggag
    15541 ccataaaagc tgcatcatat gaatcttggt aaaactccca ctggaacaat cctttaaact
    15601 gactgtccgt tgcgaccgca gcttctaatt cgccgtcttc gattgcatca tcattatttt
    15661 ttgttgcaat tggaaaagtt aattcctctt tgttattgcc gttgcctcca gagttgtttc
    15721 cagaggtatc tgtacctccg cccccgcatg ctgcaagaac gacagcagac atcagcgctg
    15781 caaatccaaa aatcgttttc ttcattccta tttccccctt cgcttctata ctagcctaac
    15841 cgttggcgcg catctgcaga acgcttgaat gcttgtccaa cataattgat actcaacatc
    15901 aaaactaaaa taaggattga tgcgggtaac cagatccatt gccgattcac taagacttct
    15961 ccgttacttg cataaccgat caatgtacct aaactaggta catttgccgg caagccgaaa
    16021 cctaagaaag tcaaagttgt ctcgataccg atgtttgccg caaagttcat cgtcaaatta
    16081 gtaatgatta atgaacttag attcggcatg atttcgcgga acatgatttt aaaatcattg
    16141 gttcccattg tttttgaggc actgacataa tctcttcttg cttcagataa tgtcttgctt
    16201 cggaataatc gggctttagc tacccaataa aatgcactca tgatgaatat aaaggaccag
    16261 acattgtatc tggaaataat cgatacgaag acgataatga tcagcatgat cggtagaatc
    16321 atgataaaat caacgatccg catcaagatg ttgtcaatga tccctccgta gtagccagag
    16381 atgatcccta atccaacccc gatgatcgaa gtaaggatcg tgatcgcaaa tccgatcgta
    16441 acagagtttc tagcacctat gatcaattgt cctaatacat cacgtccgcc ttcatctgct
    16501 cccagaatga acttttgtcc ttgtgtcgaa actgttcctg gtgcagcgta tttgtcgaag
    16561 atactaacat acatcacttt gtcttgatcg gtcagcatcg caccgataaa gatcactaat
    16621 aataaaacca ctagcaaacc taatgaaaaa atagcaattt tatctttttt aaattctcga
    16681 gcaatcattc gtatccccat aggaggaata ctttcttgaa ctgctgtatt gtttttttca
    16741 ctcatcgatc cattctcctt tctgattggt tttattgaat ccgaatacgc gggtcaacga
    16801 tactcatgat gatatctgaa agtaatgtcc ccactagcgt cgcgattcct aaaatcaata
    16861 ctaaggatgt gatcacggta tagtcccgtt ggccgatgga atcaataaat agttttccga
    16921 ctcccggata accaaaaatc ttttcgataa ctacagaacc agcaatcaac gaagtgattt
    16981 cataccccat ttgagaagcc atcggcaacg ctgcatttcg gaaaatatgt cgactgaata
    17041 ctttattcgt cggtacccct ttggaacggg ctgttctgac aaaatcgagc gatttcgaat
    17101 cgattacttc acttcgcaga tattggattg tgacggcagt cgccaaaaat gcttgggtaa
    17161 ttgatggtaa cagaatatat ttcaaacggt ctatccattg agtgataaat ccaccttgca
    17221 aacctagtgt ttgtgatcca cttgttggga accagcctaa acgataacca aaaacaaata
    17281 gcatgatcaa gccgaagata aataaaggaa cagcaaaact aaagaagttg tagattacga
    17341 caatcttatc caagattgaa ttttgatacc ggccagcaag catccccaga ggcacagcaa
    17401 tcaaataagt aaggacaact gtcaagatcg atagatataa agtgttgatg atccgttcgc
    17461 cgatcaagtc agcaacagag cgtttgtata agaaactttg accaaaatcg ccttgcaaag
    17521 catttccgat ccaacggaag tactggacat accatgggtc attcaagcca gctgcttgtc
    17581 gcatctgttc gatcactgcc ggatcctgat ttggattgat caaaccggta aatggatccc
    17641 ccggcatcgc ttgggctaat aagaagatca gaacacttaa aataataact tgcgggatca
    17701 tcaataaaac acgtcgcaga atcgttttcc acattagctt gcacctccat ctttcaacgc
    17761 cactttgtgt gtagttgtga tcgtacgcaa atcgtagact cgaccagtcg catcatagta
    17821 ttctttttgg ttttggatat actcttgttc tactcttcgt cgattttctt tgtggctttc
    17881 cctattttct acatcgattt gcggaatagc tgaaagcaga cgtttcgtat agatatgtcg
    17941 cgggtcatta taaatgtctt ctcttgtacc gatttctaca aatcgacctt tgtacataat
    18001 tgcgatattg tcgcacatgt gttttactac acctaaatca tgtgaaatga ataaatagct
    18061 gaggccgtat tcttgctgga ttcgtttcat gaaattcaat acttgcgctt gaacagatag
    18121 atccagagca gatacaggtt catcggctac gatcagtttt ggattggtag caacagcacg
    18181 ggctactcct agacgttgac gttgtccgcc tgaaaattca tgaggatatt tatatagcgc
    18241 atcttccggc atcccaacga tatctagcag tccttttacc tttttctttt cttcttgatc
    18301 gcttaaccgt tcaaaatttc ggatgggttc agcaatgata tccaacactc gctttttagg
    18361 attcaaactg gacattgaat cttggaagat catctgcaca tccttgttat aacccatttt
    18421 tttgcggttg cttcgcttcg ttacatcttt tccttcatac atgatttgtc cggctgttac
    18481 cttttctaat cccacaacgg cttttcccgt agtagatttt ccagagccag actctcccac
    18541 taaaccatat gtctttcctt tttcgataat aaaatccaca ccatctacag catatacatg
    18601 atccgttact cgattgaaga atccactgcg aatagggtaa tgaactttca ggtctttgat
    18661 tgtgattaat tctgtcatct acacctctcc tttttcgtct ctaaagtgga aatgcttata
    18721 gcaggagcaa cgtaccaagt ggttcggtgc gacttcatga aggataggct cttcttcatg
    18781 atcgcttgct ggtatccatg gaatacgtgg tgcaaaccga caacctttac gaggcatgtt
    18841 tttcagagaa ggaacgacac cttcgatcac atggagttct gcatcatgat ttccttcttg
    18901 tggtatagag ttcagtaaag agcgtgtata aggatgctta ggatgatcaa acagttcttt
    18961 tacacctgct acttcaacga actgtccgcc atacatcact gccactcgat cagccatttc
    19021 tgctacgact cctaagtcat gagtgatcaa gatgatccct gtttttgttt cttcctgaag
    19081 gtctttcagt aaatcaagaa tctgtgcttg gatggttaca tctagggctg tcgtcggttc
    19141 atctgcaatg acgattggag gtttgcaggc gattgcgatt gcgatgatga cacgttgtct
    19201 cataccgcct gatagttcat ggggatattg gcgtccaact ctcgcaggat taggaatccc
    19261 tacttgttct agcaattcta gtgcacgagc ttgacgctgt tctgcattca tctttgtgtg
    19321 ataggaaagt ccttctttga tctggtcttc gattctcatg agaggattta atgcagacaa
    19381 cggatcttgg aaaatcattc cgatatcatt tccgcggatt ttattataga gcgtttcgtt
    19441 caggtcgatt aaattcagat cgttatataa aatctcacct gtaatttttg tgttattagg
    19501 atcaagtaat cccataatcg tagtagccaa ggtacttttc ccgcacccag attccccaac
    19561 gattgctaaa atctcgtttt tgtctaaagt aaatgaaacg tcatctactg catcgtaata
    19621 gtcatctttg agtcggaatc ctgtatgtag gttctgaact tctaataatt ggttatcgga
    19681 caatcgaata cccctctctg cttcctaaat ggaagttttt cttaataaaa aaggacatta
    19741 cacagaataa atcgttttat
//
LOCUS       DAZKOB010000045        19693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000045 DAZKOB010000000
VERSION     DAZKOB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.045"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG8M6_001562"
     CDS             complement(<1..210)
                     /locus_tag="ACG8M6_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0457238.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="ACG8M6_001563"
     CDS             complement(216..446)
                     /locus_tag="ACG8M6_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457239.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="ACG8M6_001564"
     CDS             1063..1725
                     /locus_tag="ACG8M6_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457240.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="ACG8M6_001565"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="ACG8M6_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0457241.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="ACG8M6_001566"
     CDS             5145..5459
                     /locus_tag="ACG8M6_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0457242.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="ACG8M6_001567"
     CDS             5472..5846
                     /locus_tag="ACG8M6_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0457243.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="ACG8M6_001568"
     CDS             5856..6200
                     /locus_tag="ACG8M6_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0457244.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="ACG8M6_001569"
     CDS             6271..7623
                     /locus_tag="ACG8M6_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0457245.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="ACG8M6_001570"
     CDS             7683..8825
                     /locus_tag="ACG8M6_001570"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0457246.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="ACG8M6_001571"
     CDS             8850..9104
                     /locus_tag="ACG8M6_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457247.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /gene="mobT"
                     /locus_tag="ACG8M6_001572"
     CDS             9360..10544
                     /gene="mobT"
                     /locus_tag="ACG8M6_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0457248.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="ACG8M6_001573"
     CDS             10541..10678
                     /locus_tag="ACG8M6_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0457249.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="ACG8M6_001574"
     CDS             10683..10904
                     /locus_tag="ACG8M6_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457250.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="ACG8M6_001575"
     CDS             10917..11417
                     /locus_tag="ACG8M6_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0457251.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="ACG8M6_001576"
     CDS             11514..13361
                     /locus_tag="ACG8M6_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0457252.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="ACG8M6_001577"
     CDS             13364..14260
                     /locus_tag="ACG8M6_001577"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0457253.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="ACG8M6_001578"
     CDS             14310..14699
                     /locus_tag="ACG8M6_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0457254.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16185
                     /locus_tag="ACG8M6_001579"
                     /pseudo
     CDS             14686..16185
                     /locus_tag="ACG8M6_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG8M6_001580"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG8M6_001580"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0457255.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18872..19693
                     /locus_tag="ACG8M6_001581"
                     /pseudo
     CDS             18872..19693
                     /locus_tag="ACG8M6_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         6655 a         3219 c         4307 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aac
//
LOCUS       DAZKOB010000046        19635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000046 DAZKOB010000000
VERSION     DAZKOB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.046"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..656
                     /locus_tag="ACG8M6_001582"
     CDS             9..656
                     /locus_tag="ACG8M6_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0457256.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            740..1684
                     /locus_tag="ACG8M6_001583"
     CDS             740..1684
                     /locus_tag="ACG8M6_001583"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0457257.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1819..3270
                     /locus_tag="ACG8M6_001584"
     CDS             1819..3270
                     /locus_tag="ACG8M6_001584"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0457258.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3291..4127
                     /locus_tag="ACG8M6_001585"
     CDS             3291..4127
                     /locus_tag="ACG8M6_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0457259.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4127..4786
                     /locus_tag="ACG8M6_001586"
     CDS             4127..4786
                     /locus_tag="ACG8M6_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0457260.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4912..5601
                     /locus_tag="ACG8M6_001587"
     CDS             4912..5601
                     /locus_tag="ACG8M6_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457261.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5607..6563
                     /locus_tag="ACG8M6_001588"
     CDS             5607..6563
                     /locus_tag="ACG8M6_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0457262.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6607..7119
                     /locus_tag="ACG8M6_001589"
     CDS             6607..7119
                     /locus_tag="ACG8M6_001589"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0457263.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7375..7923)
                     /locus_tag="ACG8M6_001590"
     CDS             complement(7375..7923)
                     /locus_tag="ACG8M6_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0457264.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8012..9193)
                     /locus_tag="ACG8M6_001591"
     CDS             complement(8012..9193)
                     /locus_tag="ACG8M6_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0457265.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9429..10937)
                     /locus_tag="ACG8M6_001592"
     CDS             complement(9429..10937)
                     /locus_tag="ACG8M6_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0457266.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11096..11287
                     /locus_tag="ACG8M6_001593"
     CDS             11096..11287
                     /locus_tag="ACG8M6_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457267.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11490..12407
                     /locus_tag="ACG8M6_001594"
     CDS             11490..12407
                     /locus_tag="ACG8M6_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0457268.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12400..13473
                     /locus_tag="ACG8M6_001595"
     CDS             12400..13473
                     /locus_tag="ACG8M6_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457269.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13621..14139
                     /locus_tag="ACG8M6_001596"
     CDS             13621..14139
                     /locus_tag="ACG8M6_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457270.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14120..14512
                     /locus_tag="ACG8M6_001597"
     CDS             14120..14512
                     /locus_tag="ACG8M6_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0457271.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14588..15523
                     /locus_tag="ACG8M6_001598"
     CDS             14588..15523
                     /locus_tag="ACG8M6_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0457272.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15656..16435
                     /locus_tag="ACG8M6_001599"
     CDS             15656..16435
                     /locus_tag="ACG8M6_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0457273.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16428..16745
                     /locus_tag="ACG8M6_001600"
     CDS             16428..16745
                     /locus_tag="ACG8M6_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0457274.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            16989..18599
                     /locus_tag="ACG8M6_001601"
     CDS             16989..18599
                     /locus_tag="ACG8M6_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0457275.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18756..19604
                     /locus_tag="ACG8M6_001602"
     CDS             18756..19604
                     /locus_tag="ACG8M6_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0457276.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6343 a         3513 c         3952 g         5827 t
ORIGIN      
        1 tcgaatcaat gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg
       61 cttgtcaatc aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc
      121 gctcttcaca ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa
      181 aaagttctag tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg
      241 aattgttcca agataaatac ccagatactg caattactag tttggaatta gatagtgatt
      301 ggggatctta cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa
      361 ttgatgctca aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac
      421 aaaatggtca aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg
      481 aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg
      541 ataaagattt aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat
      601 cagtcaagat caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat
      661 ttcttcggaa aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt
      721 gtttgaagga gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa
      781 tcctttgatg aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc
      841 tcattcagaa gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa
      901 tcccgaacca cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa
      961 cttaggtttt tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca
     1021 aaatcaaccg ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct
     1081 tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa
     1141 tgtgcctgga aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga
     1201 agtattcagc atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc
     1261 tcattttgat cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat
     1321 caacagtgtg ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc
     1381 aaaagaaggg ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt
     1441 tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg
     1501 gacaggacaa gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac
     1561 agagcttcat aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat
     1621 tatgtcagaa aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc
     1681 atagcaaaga gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct
     1741 gttcattcct gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga
     1801 ggcaaaaagg aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa
     1861 gaatttagtt ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat
     1921 gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt
     1981 ccccagtcag aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa
     2041 gaaaaaaagg gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct
     2101 catacatcta attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc
     2161 aatgcgccag agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga
     2221 aacccttgga accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt
     2281 tcatcaggaa tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc
     2341 gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca
     2401 tatgcttgga gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga
     2461 gacacggagg ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta
     2521 tcgccagttg agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt
     2581 gattcaccga tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta
     2641 gatttccttg gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt
     2701 aaactgatca cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg
     2761 attgaaacag gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact
     2821 ttgttccaat atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg
     2881 gatgaagcag caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca
     2941 acgactgcat ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca
     3001 cagatggaac acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg
     3061 tttgaggaga gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca
     3121 gcaatcagct tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg
     3181 gctgccagag gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat
     3241 cagttccatt taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa
     3301 aaagattgtt tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg
     3361 ccgctaatca ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag
     3421 cgacttctgg cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt
     3481 atgatgtaga tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag
     3541 aatacaacgt ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga
     3601 acgattttgg tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat
     3661 attcatttga cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag
     3721 atcttaaagg gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa
     3781 aacacggagc taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg
     3841 tagcaaatgg tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat
     3901 ctgctttacc agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa
     3961 gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa
     4021 aagagatgaa agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg
     4081 tttcagtcaa accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcccct
     4141 aattgatttt tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac
     4201 attagggatt ggcttcgtgt cgtttctttt aggaaatttg gtagggttgc ttttgacagt
     4261 tttaggtctt ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt
     4321 tagaggcatt cccgcactag tcctgctgtt tctgttatat ttcgggttac cttatcaatt
     4381 aagtgcctta acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga
     4441 aatctatcga ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc
     4501 tggtttttct ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc
     4561 agtaccggca ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac
     4621 aatcacagtg ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta
     4681 tttttctctc tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa
     4741 agtgcaacag tatttagaaa aacgttctat ccatcagtca atctgaaaaa tagaaatggt
     4801 gctagcgtgg ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta
     4861 aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc
     4921 ccgctaacca tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg
     4981 gttgtagaag aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg
     5041 acaaaatttg atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat
     5101 tttcgctcta cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt
     5161 aaactatatg aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt
     5221 ccgtacacgg ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca
     5281 gcaaagaaag agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta
     5341 ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaaaagat
     5401 caattgtaca aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga
     5461 tattatgaac ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa
     5521 gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg
     5581 cctacagagc cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg
     5641 acctattagc attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag
     5701 tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa
     5761 agcaccatcc aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc
     5821 agcatgtgca cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa
     5881 cgccagaaaa tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac
     5941 agcactaccc aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt
     6001 tgataccaga tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg
     6061 actcttttca aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta
     6121 gtgatgacta tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg
     6181 aaggtagccg ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt
     6241 atattgatgc ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg
     6301 ctaaagacca agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc
     6361 gtcaccatca tcaactcttg attacttcag gaatgattga attattggct ctttaccagc
     6421 aacaaaaatt ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac
     6481 ttattagtca cctgctagat catccaaaag attggcttaa atattattca aaagttcagg
     6541 aactatatga agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga
     6601 aactggatgt atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt
     6661 aatgccgcag cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa
     6721 ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg
     6781 gaagatcaaa ttgctggaac agctgcatta gtctcaggcg tggatcccgt ttacacaaaa
     6841 atagatggta gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta
     6901 gcagaaagta ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt
     6961 gcagccagca aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg
     7021 gaaaagacca tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa
     7081 cacggcgaaa gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa
     7141 gataaagaca ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag
     7201 aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg
     7261 aattttagcg tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg
     7321 acatagcttt tgtcacgatc tctcggctta tcctacgagt attattcgat acatttatat
     7381 caataatcct ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac
     7441 taaaagtgag gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc
     7501 cagtctggct gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt
     7561 ttctttagag atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg
     7621 ccaaaaacca tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat
     7681 ttctgcttta ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt
     7741 tcccttgatc atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt
     7801 ttccccaggt cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat
     7861 agcttctgcg agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt
     7921 cattgtcatt tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa
     7981 gcttttaaaa gtaactttgc ttttttgtat tttataccag caagccagtc acaatagcac
     8041 ttaaaaagct gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat
     8101 ttcctttttc ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt
     8161 tggcaaatga aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat
     8221 taccatttgc taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga
     8281 tactaccggc atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa
     8341 agatgtaacc taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga
     8401 tcatggcaat caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga
     8461 aaccatcaag aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa
     8521 tgatcagctc atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat
     8581 aaccgccaaa taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca
     8641 tataagatcc gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca
     8701 aacgtttctc agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc
     8761 ctaaaatagc agaagctact ccattataag attccaattt gcccatcccg ttgattttac
     8821 ttaatcctag accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac
     8881 cgataatagc agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc
     8941 ctttatttgc tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat
     9001 gatcaaacat ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata
     9061 cgaaaccaag aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt
     9121 ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta
     9181 ataaataact caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa
     9241 ataaaatact aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt
     9301 tccattagaa aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag
     9361 tctataaaaa aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta
     9421 aactttttct attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg
     9481 attcctagtt ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct
     9541 tgtgctccct caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact
     9601 gcactgattg ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga
     9661 agtgtctttg atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg
     9721 tcatctgcga tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc
     9781 atcaatgaac ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca
     9841 gcagaaggat cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg
     9901 actaccacgt ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga
     9961 ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga
    10021 cggatgattt cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct
    10081 acacgattca cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt
    10141 tcaataatac gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact
    10201 ttattgtagg catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg
    10261 gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt
    10321 tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag
    10381 caagtagcga gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt
    10441 gtttcaatat actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact
    10501 ggatagtctt cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg
    10561 ataaagactt tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat
    10621 aagccataac cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca
    10681 taatcaccac ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca
    10741 gcttgatctt tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt
    10801 acacgtttcg catgcttgtc gtcaactaac ttcccaccaa attctaaata taatttgtca
    10861 tagtggttca ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca
    10921 aaaccaattt ttttcactag gttccctcct taagtactaa agcatttttt catttttttg
    10981 aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca
    11041 acttgtctag atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa
    11101 aaaaagacag aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta
    11161 tgaaagaatg cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga
    11221 aacgcagcta actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag
    11281 taaataaaaa ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact
    11341 agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc
    11401 cgctcttttt tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa
    11461 aaacagatga gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg
    11521 agccagacga actagaggag acgaacgcgg atcaggatac aggacaagaa tcgtcttctt
    11581 tttacgatat caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg
    11641 cttttgaaaa agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc
    11701 gattgaatga agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga
    11761 tgcgtaagat ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca
    11821 tctcgccaac gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg
    11881 gtattgatgc aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga
    11941 aatattccca accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg
    12001 tgatcactgc tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac
    12061 aaatgactga tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa
    12121 ttcaagaatt agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt
    12181 atattgagac gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag
    12241 cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg
    12301 gtagcaaaga gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg
    12361 agaatcctaa taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca
    12421 gaatggacaa aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa
    12481 gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa
    12541 atacatgaaa aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct
    12601 tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc
    12661 gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg
    12721 atcgaatcag attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa
    12781 ccaatctcca cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa
    12841 ccgttgaatg atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga
    12901 catttttcga tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta
    12961 ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag
    13021 acaatggaaa tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg
    13081 gatcgagtat tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta
    13141 gcccatgcaa gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca
    13201 cttcgtttga atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt
    13261 atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa
    13321 gtacgattag ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc
    13381 ttgtcgatct catctggaga ctacccacga tctgtcattg atttctttat caattacgaa
    13441 ttcttggtcc cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca
    13501 ttcagataat tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct
    13561 tttctgtatg tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga
    13621 atgtccacaa ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg
    13681 atcgcttttc aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta
    13741 ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt
    13801 ttttattttg gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg
    13861 agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga
    13921 gtctgttttt ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg
    13981 ttatatcgtt tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg
    14041 cacagtatag gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac
    14101 aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag
    14161 tatactcatt ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga
    14221 ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc
    14281 ggaaaaagaa gtggtcttca aaaaaggcga tgatttactg agtattttga aacgaaattt
    14341 tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa
    14401 aaatcaactc tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga
    14461 gatcaaattg aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata
    14521 aaaaactgtt aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga
    14581 aaataacatg gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc
    14641 taatattcta gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat
    14701 gctaaacgaa agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg
    14761 tgataaacaa gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa
    14821 atactttttc agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt
    14881 gatggaagct tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt
    14941 tatagcaata ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa
    15001 agaaatcaaa gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg
    15061 acacagtgaa atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt
    15121 ggctttagca ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag
    15181 cggattgctg atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta
    15241 tagtctgatc atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt
    15301 tgaacgtcct gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga
    15361 gattttagtt gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt
    15421 catcaatgat attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata
    15481 cattgaaaca gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat
    15541 tgaagcaaaa atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga
    15601 atttatgtta caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa
    15661 caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag
    15721 ctatatacca gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct
    15781 cttatcagga ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc
    15841 tatgcttacg acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt
    15901 acgctataca ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt
    15961 tttggctgtc ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt
    16021 ttcaactgac ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac
    16081 tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc
    16141 tgaccttgtc cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa
    16201 aagcgcacta ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt
    16261 attattccaa aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt
    16321 tatgctggag aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga
    16381 tgtcaatgag ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat
    16441 atttgctact tttagttgct tgtctgttca tcgtggcata tatcccgcgc ttgttcccga
    16501 tgctttattt tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac
    16561 cggtggcatt atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg
    16621 atattgcttg gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa
    16681 cacgttcgat ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc
    16741 tttaaactgt tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct
    16801 atccgaactg catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa
    16861 gattagtgtt ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt
    16921 ccttctatga ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg
    16981 gaatttaaat gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc
    17041 tatgtaaagt tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc
    17101 aagatggtat gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc
    17161 tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa
    17221 aagaaggaga taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg
    17281 tcatcggtat cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca
    17341 gtccgattgc taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga
    17401 ttgctatccc aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc
    17461 cttatggaac ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta
    17521 cttattactt gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag
    17581 tcgcttcttt cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa
    17641 aagacttctt cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc
    17701 gatctgcctc tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca
    17761 ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc
    17821 cagagatcgc agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata
    17881 aattgacatt gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat
    17941 taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt
    18001 cgattgcgtt attgattctt cttccttcat tgactggaat gtctttatta gctggaccac
    18061 tatacacatt gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc
    18121 tagcttcttt gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc
    18181 accatcgttt tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag
    18241 taatcggatt aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg
    18301 gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag
    18361 caaagatcag caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat
    18421 gttttgccgt tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa
    18481 tgatttattg tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg
    18541 gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg
    18601 ttaactaatc gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg
    18661 tttttctatt ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt
    18721 ctggaaaaat tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata
    18781 gttcggcata tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc
    18841 ctttgattat ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt
    18901 atggactttt aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag
    18961 ttttgggttt atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc
    19021 tttcttcagg gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg
    19081 gagtaacggt cattccttat gactctaaga tcactagtat gccgatggga cacatggtag
    19141 aagctgcctt agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg
    19201 atcgaatccg agataatacc tatgcttatc tgattgtaga tgatttgaac aatttagtac
    19261 gaggaggacg tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga
    19321 tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag
    19381 catttgctcg agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca
    19441 aactttacgt gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc
    19501 agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac
    19561 atttaggtga aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa
    19621 aacataccaa aaata
//
LOCUS       DAZKOB010000047        19499 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000047 DAZKOB010000000
VERSION     DAZKOB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.047"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1125
                     /locus_tag="ACG8M6_001603"
     CDS             <1..1125
                     /locus_tag="ACG8M6_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0457277.1"
                     /translation="SSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTE
                     GQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDL
                     YASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQS
                     KFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQY
                     YLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAA
                     TQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQI
                     IGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="ACG8M6_001604"
     CDS             1474..2106
                     /locus_tag="ACG8M6_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0457278.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="ACG8M6_001605"
     CDS             2694..4133
                     /locus_tag="ACG8M6_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0457279.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="ACG8M6_001606"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="ACG8M6_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            4895..5110
                     /locus_tag="ACG8M6_001607"
     CDS             4895..5110
                     /locus_tag="ACG8M6_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457280.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="ACG8M6_001608"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="ACG8M6_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5770..6681)
                     /locus_tag="ACG8M6_001609"
     CDS             complement(5770..6681)
                     /locus_tag="ACG8M6_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0457281.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="ACG8M6_001610"
     CDS             complement(6700..7704)
                     /locus_tag="ACG8M6_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0457282.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="ACG8M6_001611"
     CDS             complement(7701..9824)
                     /locus_tag="ACG8M6_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0457283.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="ACG8M6_001612"
     CDS             complement(9829..12276)
                     /locus_tag="ACG8M6_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0457284.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="ACG8M6_001613"
     CDS             complement(12263..12652)
                     /locus_tag="ACG8M6_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0457285.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="ACG8M6_001614"
     CDS             complement(12712..13215)
                     /locus_tag="ACG8M6_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0457286.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13452)
                     /locus_tag="ACG8M6_001615"
     CDS             complement(13228..13452)
                     /locus_tag="ACG8M6_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457287.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="ACG8M6_001616"
     CDS             complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="ACG8M6_001616"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0457288.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16212..16349)
                     /locus_tag="ACG8M6_001617"
     CDS             complement(16212..16349)
                     /locus_tag="ACG8M6_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0457289.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16346..17530)
                     /gene="mobT"
                     /locus_tag="ACG8M6_001618"
     CDS             complement(16346..17530)
                     /gene="mobT"
                     /locus_tag="ACG8M6_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0457290.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(17789..18079)
                     /locus_tag="ACG8M6_001619"
     CDS             complement(17789..18079)
                     /locus_tag="ACG8M6_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457291.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="ACG8M6_001620"
                     /pseudo
     CDS             complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="ACG8M6_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19287..19451)
                     /locus_tag="ACG8M6_001621"
     CDS             complement(19287..19451)
                     /locus_tag="ACG8M6_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457292.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
BASE COUNT         5696 a         3995 c         3454 g         6354 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    13441 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    13501 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    13561 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    13621 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    13681 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    13741 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    13801 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    13861 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    13921 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    13981 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    14041 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    14101 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    14161 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    14221 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    14281 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    14341 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    14401 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt tcttgtgata
    14461 cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct ttttcagcac
    14521 tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata tacatttcct
    14581 ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta ccgcttttat
    14641 tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt tctacccaat
    14701 ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc ccttgcggag
    14761 ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct tttaatatcc
    14821 tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc tgttttatca
    14881 gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc acataatgta
    14941 gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact gacctattag
    15001 gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct aatacttgtt
    15061 ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt ctggttttac
    15121 catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca tgtggactgc
    15181 ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg ccaatatctt
    15241 taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg atatttcgat
    15301 atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg aaaatatctc
    15361 cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa tattcagcgt
    15421 gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg cttcactcct
    15481 ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa acaacataat
    15541 acttttctaa tatatacacg acttgagggc attcctccat tctcattaca agaatttctt
    15601 aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg tcagtttatt
    15661 ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata catatatatc
    15721 cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta tatggcattt
    15781 cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt tgcatttcta
    15841 caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata gaacattcta
    15901 accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc tggatagttt
    15961 tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct ttaccgagct
    16021 tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag gcgtttcaga
    16081 gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca aactttttcg
    16141 ttcttttatt agtgccttag cttttcacta aggaacgagt cgcacataaa ttttgtccga
    16201 aattcatctt gtcactttcc tttcttttcc attgctcgat ccgctgctgc acttgcattc
    16261 atcaagcaca tcacagtaac accaagcata ctgccaccaa ataccaaaaa aatatctttt
    16321 acaattccca tgacgattcc tcttttcata taatcaaatt ttcaattgcc atactttgtt
    16381 gctccaaaat ctttttatgt cggtctgata agcgtgcatt ctgtaccata tcctggatat
    16441 aggttgtccg attcaatcga tccaactcct tagccatttt ccacgtaggg gcgacttgtc
    16501 ggcctagcca attcaaggtt cgttcaatgg tatacggttt tggctcggaa gttaattgaa
    16561 tctctttccg attccgtcca ataaaacgcc cccattgttc attcaagggc cagttggttc
    16621 gccttttacc ttctacttta tccgcaaatc gcaagtaacg attgataatg gaaaacgtgg
    16681 tgctctcaat gctacgacct tttaacaaat cttgaatcgc atgaaaagca cggtcatttt
    16741 tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc taatggaatc cctaacttga
    16801 tatattgttc atagtctttt tcatacaaac agaaataaac ctcactcttg agactgccga
    16861 tatataaggt attgcccatg ccgtcctttt catcagcctt taaaagttcc ccggaacgat
    16921 aacttttaaa ggtacgaaac aaggagatac attcttcctt ctgacatttt ttcgttagct
    16981 caggtatatc caatagcccc actcgatcat tgattgccaa gtccaagcgt ttcatcacgc
    17041 caccggcctt tagacaatcc tcgaaaaagt cgtaccagct acgcttttga gcgagtaaaa
    17101 aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa aagcacacct ttatcttctt
    17161 catgagacaa catcaccaca atatcaccca tgacatactg ttcttgatag gagtaaaagg
    17221 cataatcttc atggagcata tactcaaatt tcaaatggag aatttcttga ataatttttc
    17281 gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa cagtaacgtc aaaggcaaat
    17341 cgggattaaa gctttccagt tggtcaaaaa ttttcctcat ggtttttata gatgcggtta
    17401 cgccaccatt ttctatctga tttaagtacg gccgactaag tccacaagcc gtagccaatt
    17461 tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa tagccgataa tctaaattcc
    17521 gttgtgccaa gttctcacct ccaaattcaa ggtgttaact ttttcaaatg agttaacacc
    17581 tatacaaatt acttactatc tttgatacaa caagcattct taatggttgc ttacttattt
    17641 tttgtgtttt tgtacccccc tgttagatac gggggtgaga catcatggtc ggctaacgcc
    17701 gtccatgaac aggcacagca ccacggagga tttctgcttc gcgaatcctc cgcggtcagc
    17761 gcctaacagt cagttgtgtt ggtttaggtt actcatctgt aataccattt acttccaact
    17821 ctgctttcat ttctaacaag tattgacgaa tatctggttc ttttgcttgg tccgcaatcc
    17881 gctcaaattc tgcaattact tgatttattt caattcgagc atccgtctcc tcattcatta
    17941 aatatggatc gccagcgact ttgacaccat tcttgcgaat atcaaatttt aaccgtcgca
    18001 attgttcttc taattctggg tctgactgaa tctttccaat cacccaataa atttctccaa
    18061 tcaactcatc ataaaccatg tacatccaac tcttttaatc ttctggcaat atcaaaaatg
    18121 accggaactg ttacagagtt tccggcttgc ttatacaact gactatctga attgacttga
    18181 gaatctcgat caaaagccca atcgggaaag ccctgaagtc gccaacactc tctaggtgtt
    18241 agcttccgaa tttgatactg atccgtgacg atcgcttgtt ggtctcctgt tagtaaggta
    18301 tgaacactct gttttcccag acgacctcgg cgacttgtgg aattaggaaa agccagattc
    18361 acactgtctt ctggcttcac ctcttgatat tctgtttcat tttttcgaat catgattttt
    18421 gtaccacctt gtttcgttga caaggtgggg gcggttccag taacttcata aactcgatta
    18481 ccttgttcaa actttcccgg taatagtcct gctacagaga ttccatccct tgctggcagt
    18541 gcgattttga ttccttcatc acctgttaag gttggcgcta cgccatctga accatagact
    18601 tgtccaccca ttcctcgatt ggaaggattg atatttacca aatttctgat gttttgatca
    18661 gatgttgaag acggtccaat gataggaaat acttgttcgg tacatgcgtc tcgagaatgt
    18721 ccgataagga atatccgctc ccggctttgg ggaatgtagg cctttgagtt aagcacctac
    18781 cactccacgt cataccccaa tccatccagc gttcggagga tcgtctcgaa cgtagcccct
    18841 ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata aaagcttagg tcggagaaga
    18901 gtggtgaacc gagcgatttc aaagaataaa gtacctcgag tatcggaaaa tctttgccgc
    18961 tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc aaaggatgtc cactggtccg
    19021 agggattgaa taaactcatc tgatacactt gtgatgtcat gcatttccac ctccttactt
    19081 gtatcatgaa ttgctttata actacgtcta gcaaatttat caatttcaca aaaaccgata
    19141 caatgatggc tcgcctgttc catacctaaa cgaaagccac caataccggc gaataaatct
    19201 aaaaatctca tacattacct cattcattgc aaagaaaaaa gccaagaaca ttttgctctt
    19261 ggctcataca ttattaattt gtcctactat tttttattta atccaactaa tttccgttgt
    19321 ttttcattgg cctgatcggc tacatattta atcaccgttt tgaactcttt tgcagtagca
    19381 ttttccgata aatccgcaat gctcttattg tatttcttta caattttcgt tacttgttct
    19441 ttctcagtca tgtaattgaa cctccaaatc tttcctacta taccctagtg gtaaaaata
//
LOCUS       DAZKOB010000048        19184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000048 DAZKOB010000000
VERSION     DAZKOB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.048"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1483
                     /locus_tag="ACG8M6_001622"
     CDS             56..1483
                     /locus_tag="ACG8M6_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457293.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1575..1940)
                     /locus_tag="ACG8M6_001623"
     CDS             complement(1575..1940)
                     /locus_tag="ACG8M6_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0457294.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1937..2302)
                     /locus_tag="ACG8M6_001624"
     CDS             complement(1937..2302)
                     /locus_tag="ACG8M6_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0457295.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2494..2742)
                     /locus_tag="ACG8M6_001625"
     CDS             complement(2494..2742)
                     /locus_tag="ACG8M6_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457296.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2741..2869
                     /locus_tag="ACG8M6_001626"
     CDS             2741..2869
                     /locus_tag="ACG8M6_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0457297.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2926..4620
                     /locus_tag="ACG8M6_001627"
     CDS             2926..4620
                     /locus_tag="ACG8M6_001627"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0457298.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4720..4893
                     /locus_tag="ACG8M6_001628"
     CDS             4720..4893
                     /locus_tag="ACG8M6_001628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457299.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5271..6404
                     /locus_tag="ACG8M6_001629"
     CDS             5271..6404
                     /locus_tag="ACG8M6_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0457300.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6435..6755
                     /locus_tag="ACG8M6_001630"
     CDS             6435..6755
                     /locus_tag="ACG8M6_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0457301.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6784..7248)
                     /locus_tag="ACG8M6_001631"
     CDS             complement(6784..7248)
                     /locus_tag="ACG8M6_001631"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0457302.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7380..8633)
                     /locus_tag="ACG8M6_001632"
     CDS             complement(7380..8633)
                     /locus_tag="ACG8M6_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0457303.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8662..8979)
                     /locus_tag="ACG8M6_001633"
     CDS             complement(8662..8979)
                     /locus_tag="ACG8M6_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0457304.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9005..9325)
                     /locus_tag="ACG8M6_001634"
     CDS             complement(9005..9325)
                     /locus_tag="ACG8M6_001634"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0457305.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9789..11204)
                     /locus_tag="ACG8M6_001635"
     CDS             complement(9789..11204)
                     /locus_tag="ACG8M6_001635"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0457306.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11221..12537)
                     /locus_tag="ACG8M6_001636"
     CDS             complement(11221..12537)
                     /locus_tag="ACG8M6_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0457307.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12687..13385)
                     /locus_tag="ACG8M6_001637"
     CDS             complement(12687..13385)
                     /locus_tag="ACG8M6_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0457308.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13674..14663)
                     /locus_tag="ACG8M6_001638"
     CDS             complement(13674..14663)
                     /locus_tag="ACG8M6_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0457309.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14743..15576)
                     /gene="msrA"
                     /locus_tag="ACG8M6_001639"
     CDS             complement(14743..15576)
                     /gene="msrA"
                     /locus_tag="ACG8M6_001639"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0457310.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15751..17385)
                     /locus_tag="ACG8M6_001640"
     CDS             complement(15751..17385)
                     /locus_tag="ACG8M6_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0457311.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17372..19183)
                     /locus_tag="ACG8M6_001641"
     CDS             complement(17372..19183)
                     /locus_tag="ACG8M6_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457312.1"
                     /translation="MKIDSFLVYHTSAMKNLTQNLLKKDNSSILKIELKKENNKSMKI
                     SKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFN
                     NYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILISFYYGEKFFKKI
                     DAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSV
                     SISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSY
                     FWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPL
                     FILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMI
                     ITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRY
                     TTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKV
                     TVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISI
                     LTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT         6089 a         3752 c         3190 g         6153 t
ORIGIN      
        1 acatgtcata atatttccca tattgtgcaa aaaaatacaa ggcggaggct ctactatgat
       61 tgaagaatat attgagaaaa atattttaag acagctgttt ttatgtgggc aattctatgt
      121 aaataaagaa gtcaatctag aaaagctctc caatctcctg catgtttgca aaaccacttt
      181 actcaatgat atcaataaca taaaaaaaga atttgaagaa cagatcgctt atacacatcg
      241 ggaaaaggac cgttacactt tatatttttc agaacatata cctcgatgca agatcatgca
      301 gcaattgtct caaaattcgt tgtttttaaa aacttgtctg ctttatttgg aagaagatga
      361 acctgattat cttcaattaa ctgaatgtga attcatctct gtcagcaaag cttattcatt
      421 aaaaaaacaa gtattagctt attttaatga ctgcgggatc gaaatcgacc gctactcccc
      481 tcgattcact gaaatggaaa gacgtctgct gcttcttaat gtgtcgtatc gtttaggcgg
      541 ttttaattct tgggagctac ctgaatcgtt ttttgaacgt gccgatcgtt tcattgaatc
      601 cgtaacggaa aatagtggca gattttatga taaagaaaat aaagaaatct tatcgattgg
      661 ttttgctatc agtttcctac gccagcaagt gtgtgctgtg acaatagatt caaaatttat
      721 cgaagaaatc aaaaaacgtc ctatttacaa ttatgtcgaa tcagcatggg aaaacactga
      781 tttccaaacc tattataaaa aagaagaatt cgcctttatt ttgacactat tcaatctttg
      841 taattatggt ttccattctt atcagttgat tgcggaagac tttcaacagc tacaccaagt
      901 gtttattgac aatactcccg aaataaaaga actagttgct acctttgaat ctcacttcaa
      961 tcaagaatta ttcggaaacc aaccgttcga acgtgcgctg attcatctga tgcgttcggc
     1021 ttgggataac tatcaattat tcatgccaga aaaattttat cttttaaatg aggaacagac
     1081 aaatttatat aaagaagtcc agacaatttt ttccagctgg tccagccagt taccttatga
     1141 tctacggttg aatcctaatt gcatgcgggc ttttgtgatc gaattgagcg gaattctccg
     1201 tctgacaaaa gaacatctga ctatctatat cgtcacaaat tcggatgttc attatctgat
     1261 ttatcgggaa gccttggaag ctgtaactac tttcgatttt caagttgcac caacaattta
     1321 ttcaagtata tctgatataa agaagtatgc gcagcagtcc tcaaaccggg tattatgtga
     1381 acgtacatta tatacaccag atgcggtcca atatgaaaat attattccta tttcgatcaa
     1441 tacgatcgat cgagctatca tttcagctgt gcaaaacaaa taaggggctg tgacaaaaat
     1501 catgtcacag cctcttattt gttgaagatg cccatttctg taacaatctc ttgatacacg
     1561 tcaggctcac aacctcactt attttggaca tctacgggtc cgatctcttt atttaaaaaa
     1621 taagaaatga tggttcggat aactaccaac gccgctagtt ggaaaatatc ctgcaaggta
     1681 ggtttgataa tcgattcgat gatcccagct gaaacaagaa tttccaaact tagaagtata
     1741 tagcctccca acatcttttt aatagcattg ttctcgctga ctcggtcgct ttgcactttt
     1801 gtatggacaa ataaatattt caaaaaatcc ttgattgata gacaaacacc ccaaaccaat
     1861 aaacagatag caaagatatc aagtcccagt acaagttttt cgaaatatgg aagaagaaac
     1921 tgcatcaact ctgtcgtcat cctacctctc ctcttctttg tttttctttg tcttctattt
     1981 cctgattcaa gaaataagaa atgactgttc gaatgataat aataagtgca agcttcaaaa
     2041 tatctgtcca agtcggcttg ataactgact cgatgatatc agcaacgact agtacttcca
     2101 agcttaacaa aatataactt gctagcagtt ttcttgtatc tgcattacga gtattacgtt
     2161 ctgcatagtt tttcttgaca tgagaccaat caaaaagttt gaatagaaca agaacgatcc
     2221 cgacaattaa gataccgata gaacaaatat ttaaaatagc aatgatccat tctagaaatt
     2281 caattaaatt tgagcgaaac atatacgcta cctactttct tttaatttgt tcctattctt
     2341 ttattgtaaa agaaagttac cttgttcgtc tatttaaaag ttgttctaat gataacggtt
     2401 cgcttaaatt tttctttttc actctcccat ttttctccct ccatccataa atcgtggaag
     2461 tatccaaaac aacgtagcaa gttaaaataa ggcttagtcc gtcatcagct tttcaaagaa
     2521 gcactgtctc ggtgcttttt ttgtacccaa atttctaaaa atagaaccat acacctcctc
     2581 aaatcttttc ttcttaccta ccctattttt atttacagga aaatcagaca cttcttcttt
     2641 tttgtcaaaa tttccttttt cttctcgtcc tatcatttgg cattctaata aaaacgaact
     2701 atccttggat atagaaagag aaaacaagga ggcgtttttc atgagtaaaa aattaatcaa
     2761 agtcggcatc ggtttaggtt tgcttgccct tggcgctgca tatctaggaa aaaagacagg
     2821 actattcgaa gatgacagtc atttatatga tgaattcgaa tcaatctagc atcctattta
     2881 gttgaatcac tacggacata cttggaagac attggaggaa tcacaatgag aaaaagtaaa
     2941 gcaataatga ttggtgccgg tattgccaat atggcagcgg ctgtttattt gattcaagaa
     3001 ggcaaatgga aaggggatca gatcaccttt tattcattgg atgatcacgg ttcaaatgat
     3061 ggtgcaccga ccgacactgt agtggatgaa tattggaata aaaaccatcc attagaaaac
     3121 caaaaaggct atattgctcg aggcggtcgc atgcttaatt accgtaccta tgtagatttg
     3181 atggatctgt taagtcgaat accttctgtt actgagccag ggatgactgc tgaagaagat
     3241 acgagagatt ttgatgcaaa acaccgaacc tttgacaaag cacgtttgat ggaaggtggc
     3301 aaaggaatca ttgatggcgg aaaactggga ttcaataata aagaccgtat tcttctgact
     3361 aaactgattg ctatgcctga ttcagaagaa gaaaaactag acaatatcac tatcgaagaa
     3421 tatttcaaag atgatccaca cttttttgaa acaaacttct ggtttatgtg ggaaacaaca
     3481 tttgctttca gaacacgtag ttctgctcaa gaattgcgtc gctatatgca tcaaatgatt
     3541 tatgaattca ctcaaatcga acatctagtg ggcgtcaatc gaacacgtta caatcaatac
     3601 gaaagtatta tgcttccttt gatcgagtac ttgaaagaac aaggatgcaa aatcattttg
     3661 aaccgccgtg tcactgactg ggaattcaaa gaaacaccga tgcaagatga aatcactgtg
     3721 actggtttga aaattaccaa tacactgact caagaagaag aatacgttgt ggtcgatgaa
     3781 gatacagcag ttattttcac taatgggtcc attacagatt cagctacttt aggagattac
     3841 gaaacaccag ctgtagaaaa tatggattat ggtgcagctt ctagtctctg gaaaaaagcc
     3901 agtgaacgtt tctacaactt gggaaatcct gacaaattct ttgctgatcg agatgcaagc
     3961 gaatgggtca gctttaccct gacgacgaaa aaccatttgt tgttgaatga aatcacccgt
     4021 atcacgactc aagaaccagg aaatgccttg aattcattta tttcaaccac accgatcacg
     4081 ccgttaggcc aaaaagatgt caatatgtct attgtcgtgc atcatcaacc gcattttacg
     4141 tctcagaaac caaatgaaac agttatttgg ggttatttcc tttatccaag acgacgtggc
     4201 gaatttgtgg ataaacagta tattaaaatg aatggaaaag aaatgctaga agaactgatt
     4261 ggacaacttt caaaagtaga tcctggacca gtgaatattc gtgaaaaaga aacagaaatt
     4321 ttcgacagcg taataaataa tatccctgtc tatatgcctt atgcttccgc gttgttcaat
     4381 aatcgtgcca aaagcgatcg accaaaagtc attcctaaac attcgaccaa tctggccttt
     4441 acaggagaat ttgtcgagca gccttatcag atgatcttta ctgaacaaag tgctgtacgt
     4501 tctggagaaa tcgctgcttt ccattttgcg ggtattccta tgtcccgtct tgtcaaaaca
     4561 ccgcgttacg acaaagatat tcctactcta gctcgtgccg caaaaaaaat gtttgaataa
     4621 tcaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacaaccag gctaaaacag
     4681 cttcttatgt ttttgatttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt
     4741 ttaccaagag gttatgaaaa agctgccctg catcaagtaa taagagcagc caagctaaaa
     4801 cagcttctta tgtttttgac ttggttagac ttattaccgc agatgcaagc ttttgaacac
     4861 cgtttaccaa gaggttatga aaaaggcgtt tagtttcgag tatgaagaaa aattttgtga
     4921 aaatggttcc tcacgttttt gcataaattt gacttaatac cgagaaactg atcccttctg
     4981 tcataacctc tttttattta tgtctgtttc tttttgaaaa caactatttt ttcaacgttt
     5041 actctcttgc caatccaaca tcatctaacg gatacaacta tttcaatgat taccattaca
     5101 ctaaaaaatc ggaaacacac ctctccgaac acaaatacat aagactatag aatgtttcat
     5161 ttaagaaaaa aagcgtttca ttatcaaaaa ggtaagatct cctttaaaat gaaattatct
     5221 gtagtactaa taatgcagac aattttatga atctgaaagg agcatctcca atgaaagcag
     5281 tcacttggca aggaaaacag aaaatggaaa tcagaaacgt agatgatcca aaaatattag
     5341 aaccaacaga tgcaatcatc cgtatcacag caacagccat ttgcggctcc gatctgcatc
     5401 tttaccatca tggtgaagca gttttggaaa aagggtatgt tgtaggacac gaaccaatgg
     5461 gaatcgttga agaagtggga tctcaagttc aaacattgaa aaaagggcaa cgtgtggtta
     5521 tcccttttaa tattagttgt ggtcattgtc atttctgcct gaatcacatg gaaagccaat
     5581 gtgatgaatc aaatccagat cagttatatg gcgggctttt cggatttggc aagttgaacg
     5641 gtaatcattg gggtggtcaa gcggaatatc taagagttcc ttatgcagac tctacttcat
     5701 ttgttgtacc agataatgat cttcctgatg aaaaagtttt atttctatca gatattttgc
     5761 ccacagctta ttggagcgtt gaaaacgcag gagtaaaaaa aggcgataca gttgtgattc
     5821 taggttctgg tccagtcggt ctattcgctc aaaaattcgc tgttatggct ggagcagagc
     5881 gcgtaatcgc agtagatcca gtacagcacc gtctagataa agcggctaat tacaatgggg
     5941 ttgaaactta tttcttagaa gacacggcac aagctggaac agaattgtat gaactggcta
     6001 aaggtggcgc agatgtcatt atcgattgtg taggaatgga cggattagag cctgtcaaag
     6061 aaaaagcgaa aaatttagta agcttacaaa gcggcacaat ctctccatta cagatggcat
     6121 cgcaagctgt caaaaaattt ggaacagtcc aagtaactgg tgtttatatg actccagctt
     6181 cttcttaccc gctgcaagaa ttttttatgc gggatatcgc tgtcaagcat ggacaggcac
     6241 caattatcca tctaatgcca aaaatttacg caatgattgc agaaggacta ttcgatccta
     6301 cacaaatcat tactcattcg ctgccactat cagaagcagc acaagcatat gagatctttg
     6361 acaaaaaaga agataaaaat ataaaagttg ttttgaaacc ttgaattcag taaaaaatac
     6421 aggtgatttt ctttatgccc aaaatcaaac gaaataccca tgagatcgaa ataggaaaag
     6481 aagaagatat cgaaatacga ggacgtcgct tccgcttcta tttccaaaat cgttacacat
     6541 tgatctcact ggctgtcgat ttattaaccg ggattttcta tatttttgga agccttgctt
     6601 ccttaactaa aatccccgat cgatatggca cctattttta tttggctggt gctattttct
     6661 tgacgattcg tcctattctc agaatcgtaa ggaacatttt tatttacgac gaaaaaaaag
     6721 caatgaagag aacaagcaaa aaaaaagagg catgagaatt tttcaaatct catgcctctt
     6781 ttttcaccct atcgtatcac tttgatacag acgagcggtc gtcccacttt cttccttgac
     6841 cattgtcaaa aatgtccact cgtatcgttc ataaaacgtg gtatgatctg taattaagta
     6901 aagtttttcg tacccaagtt gatgcatttc ttttctgatt tccgctaata tccgcttggc
     6961 tatcccctgc ttacgctttt ccggttcaac aaacagggca caaagatttg gtgtcagatc
     7021 agggcggtta tggaagtcgt tttcaatgac gcccgctcct ccgacgatgg tttctttttc
     7081 atctaagacc acataccatt gaggaatact ttccttatta gcgattccag tacgaatact
     7141 ttcctcatac acaacagcag gaactcccca ttttgatgaa aaccaattcg cgctttgttt
     7201 tactaattct gggtgttctc ttaatttgat ccagttatat gtaaccaatg tttaaacctc
     7261 cgactaaaat aaaacgttta caaaaattat acaagtcttt atgaaaatta gcaatgacaa
     7321 caatagtgat gtaaaaaaaa aaacggaaaa caatcacttg ttctccgttt tttgattaat
     7381 caagcatcta aagcttcttt tcttctttct tggatcgtag ctaccttgac gaacggtaga
     7441 tagatcaaaa tagaaagaac caaattgaca gcggctagta caccacctgc aatacttttt
     7501 gttgccaata caccgccaat aattggtgga gttacccatg gaggcataac ggtagctgct
     7561 ggcactaaac ctgtagaagt cgcaaagtaa gcaacactaa ctaagaccat tggtgtcaaa
     7621 ataaatggaa tgaacatgat cgggttcaac acgataggta acccaaacat cgttggttca
     7681 ttaatattga agacacctgg cgcaatcgac aagtttgtta caaccatata tggtttgttt
     7741 ttacgtccta ctaaataaat agctaacagt aaacctaaag ttgcacctgt tcctcctaag
     7801 ttgacaaagc tgtcaaagaa aggtttgttc acgataaacg gtgcgctatg tccagctgcg
     7861 attgcattaa cgtttgcttc aatggctggc gcattgattg tttgcatcaa aggatcgacc
     7921 atattcgctc cgtgtaaacc aaagaaccat aagaatggtg tgataaatgc aagcagtaag
     7981 gcagatggat aagagtttgc aagtcccata aatggttctt ggaccactgt atagaaagaa
     8041 cctacgatat catcaacacc gaatgcagca aaaattgcag caactaaagc aaacgcagag
     8101 atagtgatca ttgctggtag taatgaagca aatgcttttg caactgccgg tggcacacca
     8161 tcaggcattt tgatgatcaa tttttcattt ccgcttaaac gtacaaagat ttctgttgaa
     8221 ataatcccaa caataatagc aataaacaaa ccgtttgagc tcatacctaa tgctccgcct
     8281 aaagcgaaga aagagcctaa agaaatcaca cctgcggaaa caccgtcttt tcggtagccg
     8341 ttagctaaat gataagcaag caagaaagca atcaagacag aaagaatggc aaatgtaccg
     8401 ttccaagcag cagccccgaa tcctttccat gattcaccac caaaaattga gttcatcaat
     8461 tcttggaacc cgggaatcgg taagttattg atcattactg ctaatgctcc taagatcatt
     8521 aacggcatac tcaccataaa tgagtcccga atcgcaacta aatgccgttg tgcaccaatt
     8581 tttgatgcta caggtataaa atgtttctcc ataaatgcaa caaatttatc cataaatctt
     8641 ccctcttttc tttactgtag attacgcgtt caatcgacga tacagatcaa tgatctcttt
     8701 tgctaaatct gtaaaagcta tagatgtcat cagatgatct tgactgtgga ccgtcaaaag
     8761 cgtgacttca acgtgatttc cttgtgcttc ttgtgtcagc atctctgttt gagagtgatg
     8821 tgcttggact aaagattcct ctgcatcttt gattttttca tctgctaaag tgaaatcacc
     8881 ttttttcgca gcagcaatcg cttccattgc atcgcttttt gcatttccgc caaacatgat
     8941 cagacccatg atggcttcta gatttttttg atcttccatt tattgtatcc tccgataatt
     9001 tttattattt gtttattagt ttttctgctt ggtcgagtac tttttcacca ttcatcatgc
     9061 catagtcagc catattgatt acatctaaag gaatgccttt aggtgctagc ttttcagcaa
     9121 attgattttg cataaagcgt acttgcggtc ctaaaagtaa aacgtctaca tttttagatt
     9181 ctagttgatt atccgcatct gaagcagata cagcaaagat gtctgcttcc attccttttg
     9241 cttctgctgc cttttgcatt tttgtgacta ggagacttgt actcattcca gctgaacata
     9301 caagcatgat tgttttcttt tccattttaa acacgttcct ttctatactt acacttgtat
     9361 ataagcaagt agcgtgccaa ctttaaaagc gctttaaaaa gttgatatca tcacatttaa
     9421 ggtctatacc aagttgcata cacactaaaa aagaaagcgc acactaaaca cacaaattaa
     9481 tttttattac cttttttctt ttaacgtgca caaaaaaagt tgtgacacaa aacttgtcac
     9541 aacttttttc aaataaacgg tattcaaaag cttgcatctg cggtaataag tccaaccaag
     9601 ttaaaaacat aagatgttgt ttttactttg ctattcttat tacttgatgc aggacagctt
     9661 tttcacaacc tctcaataaa cggtgttcaa tcagctgacc ctgggtatta gctcaaaacg
     9721 ctggattcac aacctctttt atttcaaata accagtaaat acaatgaaca tctacaaaaa
     9781 acttctattt atttatagat ttcgtcaggc aaatccaatt ctctcttatc aatcaaagtc
     9841 tgataccacg aagcagattt tttcaatgtt ctgcttcgtt catcgttcaa ttcgattcga
     9901 actaatccat atcgattctt aaatgcattc attggtgaca cacagtcagt gaatgcccat
     9961 aacatatagc cttcgcaatt tgcgccttct tctgttgctt tgatcaagta atgcaaatgc
    10021 tgtccgataa attcgatacg gtaatcatct tgcacgattc ctttctcatc catatactgt
    10081 tcttcatatt ctcttcccat accgttttct gttatcagcc aagggatatt atgataattt
    10141 tcttttagat acatggctat atcatacatg attttcggat agatttccca tcccctggaa
    10201 ttattcattt tcttcccagg taaatcaaag acttcataat atcgttcagg atggaaaggt
    10261 gtctcttcat tccattgata acgaggggcg cgaacgcgtt taggataata ttgattgatt
    10321 ccaaggaaat ctactgtatg atgctgaatc gtttctaaat cctctgttgc aggatcgaaa
    10381 taaatcgatt gtttttgaca caactccagg aactcttctg tgtagtatcc ttttaccatc
    10441 ggatcaaaaa atactcgatt ataaaacaaa tcatacatat aagcagcttt ttggtcttct
    10501 ggagaattgg atcgagcata gaccatttca ggatttaaaa tacagccaat ccttccacca
    10561 taatttccgc tgcggtaaat ctctactgct ttagccgttg ctaaaacttt atgataattc
    10621 cacatcatcc acttttttgt gttttgttca tgcggccagc gaacagcatc caaatagcaa
    10681 cgcgtttggg gaacgattgg ttcgttgaat gtaaaccagt tttttaccct atcaccgtaa
    10741 cgatcaaagg caatttgagc atacttagca taaaaatcaa ctacttttct tgaagaccag
    10801 ccatcatatt tttccaaaag aaaagctggg acttcataat gttccaaaca aatcatcggt
    10861 tctacgtctg cttcgatcaa agagtcgatc atttcactta tatgctgagc atattcttca
    10921 tctactatac agttctcata atcagtaaaa aaacgagccc aattgataga agtccgataa
    10981 tgtgtcagat ggatggattt catcagatcc acatcctgct ggtattgttc cataaaatta
    11041 gttgctacgg ctgggccata cccattatgc caaacaaaat gttcattttt ataccagcta
    11101 tctataaacg aatcctgtcc ttcttttttt cctttccaac cttccgtttg ccaagcagaa
    11161 gatgcagcac caagaataaa attttttgga atagtaattt tcatgatttc ctcctgtttt
    11221 ttagatccca tcattttcca cacgatttgc tacttttaca aatggcagat agatcaaaat
    11281 agaaacaata atacaaacaa gttgcgtaat aactgcaccg atatttcctg ctgttgccag
    11341 ataagcattg acgatcggcg gcattggcca agggaccatt acaactgctt tcgccgcaaa
    11401 tcctaaagcg gttgcaaaat aaccaatcgt tccagtgata agaggcgtta agataaacgg
    11461 aatagctaaa attggattca gcatgatcgg cataccaaaa ataactggtt cattgatgtt
    11521 gaagatccca ggagcaaggg ataacttggt gatttgtttc atatcttccc gttttccaac
    11581 taacaatact gcaatcagta aaccgatcgt gactccagac ccgcccatac tcatatacat
    11641 atcccaaaat ggcatagtaa taatgttcgg tacttctttt ccagcttcaa aagcttccgt
    11701 atttacagca attgccgcta aaagcaacgg ttcccgtatc ggcttcacca tttgatttcc
    11761 atggatacca atcacccaaa agacttgcgc aataaacatc aatagcaaaa tacccggtaa
    11821 cccttgtacg acaccttcta atgggcgttg tacgacattg taaatgatat cataaaggta
    11881 cattcctgta agttctttga tgataaatcc cgcagccgcg atacccgata cagtcagaat
    11941 cgtcgggaat aaagcagaaa aactagcaga aacattcgtt ggcacggtat caggcattct
    12001 cagctgtagt ttcttctgtt tcctcaacca gcaataaagt tcaatagaaa ggatggagac
    12061 caacattccc aaaaataacc ctttcgtatc tgtatattgt ttagccaata cgttttctac
    12121 tataacagtc tgattttcat tcactaattc ataaacagtt ggattaacag ataaataaga
    12181 catcactgcg atgataccag ggaatacacc atccacttta ttcagcttgg ctaattcgat
    12241 ccctatcaga aaaacagcat agatggtcaa gaaacttagt gtgacatagc tgatggcaga
    12301 tgcgataggc tttaattctt ctaaaaaacg caagaaactt atctgtgcta agccattttc
    12361 cgagtcaaag accattgctg aaaacaatgt gccaaaagca cccgtaataa ttaccggaat
    12421 caatgcagca aatgcgtttt ttatcaccat gatatacctt aaattattga ttctcgttgc
    12481 cacgttccct aaaacattgg ctaatttatc cataaaactc attccagatc ccctcattcc
    12541 tgcttcttct ccttgacgtc atctcttttt gtctattagt cagcgcttac atttcgatga
    12601 acagaaacta tttactcctc ttttttacca aatataaaac aaaaattcat ctgatgtatt
    12661 tatcataaaa tacatcagat gaactgtcaa ttagtttctt tttctcctat tattttctgt
    12721 aattctctcc gatttattgc catatggaca gtcattgcat caaacgcagc aagtgcattc
    12781 cgcctttcga tggcctgaac gatctctgta tgaatatcga tcgtctcgat tttcagatgc
    12841 tggatatcat aatttttatt aaacaaagca attgattcat taatgattgg gataatatgc
    12901 agcaaggcta cgtttccgct agcttctgca atcatcgtat gaaaatcgat gtctaattga
    12961 acatgttctt gtgtgccttc tcgaaaagaa gtttcaatct tttgcgttaa ttttttcatt
    13021 tggacgattt gattcttcgt tgcatattcc gctgccaaac ctgccatctt aggctccaat
    13081 aaatatctca gttcaaataa gtctcgtatc atccgatcct gatcttcaat caaagaaaaa
    13141 ccgagtgggt cattggcaat ccctcgctta tcactgacaa atgtccccgc tccttgccga
    13201 acttcaagga tattcctgga agctagagcc cgcacagctt ctcgcaccgt gcttcgcccc
    13261 acctctaaat aatcggacaa ttcatattca ttcggcagtt tatctcctac ttgcaactgc
    13321 atttccttta aatatctaag gatcttgtca gtagttactt ctactagggt tttattttct
    13381 tccaaaccac atacctcctt ttcatctgat gtattttttc gtaattatta taacacgttt
    13441 gaatcatctg atgatttttt gtatacaaaa ttgattaatc cgttcataga catacaaacg
    13501 aagaagatat ttaccataaa aaagactagg ataaaattca ctcctagcct tcgatcattt
    13561 ttattttgaa cgacaaaaaa tatgatgttc aataacagac gatcttcatt cgattcttga
    13621 tgtcatcttt tgtttttttt aatatatgga taaaaagttt gattacatcg tttctattct
    13681 agaatttggt cgattctcaa ttcttcttct ggtgagaaat ctaaattatc catcattcga
    13741 acactttctt gtaactgact taaccgactt gctccgacca gtacacttgt aacagatttt
    13801 tgtcgcaaat tccatgcaag ggccatttgc gctaatgatt gtcctcgtga tacggcgatt
    13861 gtttgcagtg ctttgatttt ttccagagtt gatccaactt gttcttctga aaggaatggt
    13921 atttccttac gatgtgctct agaatcttca ggaataccat gtagataacg atttgtcagc
    13981 aacccttgag caagcggact gaacgtgatt gcccctagcc cttcttcttc taatactgga
    14041 gatagatcgt cttctagtag tgcacgagaa aacatattgt agcgcatctg atgaataatg
    14101 aacggtgctt cctttctttt taaaatttca gtcatttttt ttgtatcttc tccattatag
    14161 ttggatatac caatatataa tgcttttcct tgtcttacaa gttgcatcaa agcttctgct
    14221 gtttcttcca aaggtgtatc agggtctggg cgatgatgat agaagatgtc aacataatct
    14281 agccccattc tttggagact ttggtcacaa cttgaaatga tggatttacg agagccccat
    14341 tccccatatg gcccttccca catatgataa cctgctttgc tagaaataat cagttcatca
    14401 cggtaggctt gaaaatcttc tctaaaaata cgcccaaagt ttttctctgc agctccagca
    14461 gggcctccat aattattcgc aaggtcgaaa tgggtaattc ccatgtcgaa tgcgcctctt
    14521 aaaatctctc tttgattatg gataggatca taatcaccaa aattttgcca taatccgaga
    14581 gataaaatcg gtaatttcaa acctgaatga ccggatttac gatattccat tttttcataa
    14641 cgtgcactat ctgcaatata caatttttct cctcctcatc aaatcttttt aaaacgcttt
    14701 cttttttcta tttttagttt actacatttt aaagaaaagg agttaatcaa aatgattttt
    14761 tcgtatattt ttttgccatt tgttttttac tcgttgatag atcagaaatt gattacgtgc
    14821 ttgctggatt tttttataac gtttcggttg ttttttgtaa aattgctgat gatagttttc
    14881 tgctggccaa aacgtaccgg ctggttcgat ttttgtgacg attggttggt ggaaagttcc
    14941 tgaactgtct agtttctgtt ttgattgttc cgctaattct ttttgtcgtt cgacttgata
    15001 aaaaatcact ggacgatatt gcttccctct atcttgaaat tgtccaaatg tatctgtggg
    15061 atcagtaatt tgccaataga ttgtcagcaa ctcgctataa cttatttctc ttgtgtcata
    15121 aatgatctcc acagcttcca catgacctgt gtaaccacca cttacttgat cataatttgg
    15181 tttttcaaca tgtccgcccg tgtatcctga caaaacagat aggatccctt ttttcgattc
    15241 aaacggctcg accatacacc agaaacatcc ccctgcaaat actgcacgtt cttgatagtc
    15301 agcatcttgc atggcatgct tggaaaagtc atatttttgc tccttatcaa atccttccgt
    15361 aatcaataaa taaaagtcca ttacatcagg cgtcagattc attcggatag ctaatggacg
    15421 tagtgttgct tctagttttg ataactgttc tttaacggat atattttctc ttatgtcatt
    15481 ctttgtctga atcaatactt ttcgttccca atctcgtgta ttcggattca aaatcagatt
    15541 ataaagtgtt tgaatgaata tttgttcctc atccatagat tctcctcctt tccgaagaag
    15601 acaagctagt gactttagct gtaatttatc ataaagtgag gaaataagcg attacttcat
    15661 ccccctacag atttttcgta taaaaaagag agaatcctta tcgacagatt ctccttttta
    15721 atcaatttat tttataaatg aaaagttaat ttacctttta tggaaaacta ttttaaaaaa
    15781 taaacataaa tatgcagtta atgtaacaag ataagaaata atagtcgtta ctgtataact
    15841 tgtgcttact ttaatatcag caactccttt gactccgctt atttgtacca atccattagc
    15901 attcacctgc tcctttacag gttgatgatt tatttctaca ttcgttccta aataagcata
    15961 tacaggaagt gtgattactt ggctcttctc attagttact ctatatatga tagaactacc
    16021 agttgtttta taaggaaggt ttacccattg actgccaatc tttgcttcgt gttttattat
    16081 atgctcctta ttctccaatg aagcgatagg agaataatca tataaaaaat aatccattcc
    16141 tttagtggta tacgcctcta ctgtttcttt gtttacaaca atatgtcccg gctgagaaat
    16201 tattttttga ttgaaatttt ttttactaga aacagttaaa gtgattaata acaaaacagt
    16261 cataaataaa taaaatagtt ctcttgtctt attgtatctt ctatttttga aaaatatcat
    16321 cgttaatatc agagaagaga atattatttg caagcttaag atccgccaag gaaattgtaa
    16381 caatttggct ggcgtatttt gcagaagata ccatggcacc aaactagttg tagctagcca
    16441 agtcaaacac gtaccataat aaatcttttt ttctatgttt gttaattttt taaataaaat
    16501 caggggaata cataaagata acagaccaac tagtcccatt gagtaagtag ttaagtcgtt
    16561 gttcatagct tcagtcagac tatcgaacac attagatgca tgagcatata aatttgtcac
    16621 gctaggtttg ttgatttttt ggtagagcat ttgttcaaac atcggatacc aaaaagcact
    16681 cgtcagaacc aatgtcattc ctgccgcact aaataatttt ttaaatatag caataaaatc
    16741 tgcattcttg aaacctttag aaaccaaacg aataaaaata aaaatgataa ttaatgctgt
    16801 actcattaat gttgatagta catggctgta cactagcaaa ctcatactca atgctagctt
    16861 tacagacgga tacttttctt cttttccaga gatgatttga taagcataaa ataatacagg
    16921 gataacaagt gttattgcga tagcttcggc aattgcggaa cgatagtaga tgtctactgt
    16981 ccgataagca gagaaaatat agagacaact aaataaaaat gccgcttttt tttgttttaa
    17041 aaaccgctcg ccataatgat aagctacgag tgttgtacac aagttcaata accaaacgta
    17101 aataatatag cttacaatca aattttttgt catccaatat aacattacag ctggaaaaaa
    17161 agtcagaaag ggataaaaat aatttacacc atatccagta taattgaatg tctcataatt
    17221 aatcggactg gtaaaaatag tcgataaccc cttgattcga gataagtgaa aagaaagatc
    17281 tccagtggta taaatactct gtgtattttt gaaaaagatg aaagaatata tactactaac
    17341 tataattaat ccactcaata ttactacttt tttatttatt ttcattcttc ttgtttatct
    17401 cctttttagt cacatatcga gaatctttca acttattagg tgcaaatcgt ttaaaacttg
    17461 aatacaccac taaacaataa gtcagaatac tgatccataa agccatgaga gcagttggag
    17521 tatattttgt tgtaatttct atcttatttt ctccagcatt tacatccaca ttcactaagc
    17581 catgtttttt ataagagtca tttacgactt taccgtttac ttttacctgt gtacctaaat
    17641 aagcatatac tggtactgtc actttttgct ttttgttgct tgccactgtg taagtaatgt
    17701 tattatctgc agctttatac ttcccttttt cccacgattc tcctactttt acttcatgtt
    17761 tttctaagtg aggcctttct tttatcgctt ctgttggggc gtaatcgtat aatcctccca
    17821 tatctgatgt tgtataacgt gggagtgttt cttcattgat aacaatatga ccgtgttttg
    17881 attggataac ttttgcgtaa ttttcttttg ctgctataga cgtgacgagt agaaacacca
    17941 cagtagcgcc aatgattaac catgaatatt ttttattcat agtctttttc ataaaaacaa
    18001 gcgttatgat cattgaagaa aaaaagattt ggatacctaa gatacgccaa ggaaactgga
    18061 gtaagcttat cggtgttttt tgaaacaaaa accatggaaa taaattagtt acgcataccc
    18121 atgtagaaac tacgccaaca tagatgattt tttctttttt ctccatttct ttcagtatga
    18181 ataaaggaaa aatcaacgca accaatccta ataaccccat agtgtatgtt gtaatatcgt
    18241 tatttaacgc ttgaatgata ctatctgaaa tagaaagtgc ttctagctgc aaatctgttt
    18301 tgaagggact gtgtatttct tgataattca tctgctgcaa cataggatac caaaaatagc
    18361 tagttaatgc acaagtcaaa aaaacagcct ttactaaatt cacgaccaca aattttatat
    18421 cgttttttat ttgcttgctt aaaaacaaac gaactataat catacaagca atcaatgcac
    18481 tacacaatag tgtagttaag acatgagtat acacaagtag acttatactg acagataaag
    18541 cgatgacttt tttttctcta ttgaatatca atccataagc ataatataaa acagctggga
    18601 taatcgtaag agcgattgct tctgcaattg cggatcgata atagatatca actgttcgat
    18661 aagttgcaaa agtatataaa caactaaata aaaaagcagc atctatcttt ttaaaaaact
    18721 tttctccata ataaaaacta atcagaatcg tacaaatatt taataaccaa atatatagaa
    18781 cataacttaa tattaagtta tttgaaattc cataaaaaac gactgcagga attattgtaa
    18841 gaaaaggata aaaatagttc agtccatctc cataattgtt aaatgttgta tagttgattg
    18901 gcccttcaaa aatagaagat aaccctttga ttcgtgaaag atgaaaagat aaatcacctg
    18961 ttgtataaat ttcttgagca tttctcataa aatttactga atataaagta ctaagtaaga
    19021 taagaactac taaaagaaat gcttttttac ttattttcat agatttgttg ttctcctttt
    19081 ttaattctat tttcagtata ctagaattgt ctttttttaa taagttttgc gttaaatttt
    19141 tcatagcact agtatgatat actagaaaag aatcaatttt tatt
//
LOCUS       DAZKOB010000049        18978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000049 DAZKOB010000000
VERSION     DAZKOB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.049"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..2185
                     /locus_tag="ACG8M6_001642"
     CDS             32..2185
                     /locus_tag="ACG8M6_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0457313.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2154..3569
                     /locus_tag="ACG8M6_001643"
     CDS             2154..3569
                     /locus_tag="ACG8M6_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0457314.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3602..4636
                     /gene="sppA"
                     /locus_tag="ACG8M6_001644"
     CDS             3602..4636
                     /gene="sppA"
                     /locus_tag="ACG8M6_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0457315.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4638..5159
                     /locus_tag="ACG8M6_001645"
     CDS             4638..5159
                     /locus_tag="ACG8M6_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0457316.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5156..5542
                     /locus_tag="ACG8M6_001646"
     CDS             5156..5542
                     /locus_tag="ACG8M6_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0457317.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(6038..6110)
                     /locus_tag="ACG8M6_001647"
     tRNA            complement(6038..6110)
                     /locus_tag="ACG8M6_001647"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6075..6077),aa:Lys,seq:ctt)
     gene            6491..7060
                     /locus_tag="ACG8M6_001648"
     CDS             6491..7060
                     /locus_tag="ACG8M6_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0457318.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7047..7907
                     /locus_tag="ACG8M6_001649"
     CDS             7047..7907
                     /locus_tag="ACG8M6_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0457319.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8097..8801
                     /gene="yycF"
                     /locus_tag="ACG8M6_001650"
     CDS             8097..8801
                     /gene="yycF"
                     /locus_tag="ACG8M6_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0457320.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            8806..10641
                     /gene="walK"
                     /locus_tag="ACG8M6_001651"
     CDS             8806..10641
                     /gene="walK"
                     /locus_tag="ACG8M6_001651"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0457321.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            10638..11954
                     /gene="yycH"
                     /locus_tag="ACG8M6_001652"
     CDS             10638..11954
                     /gene="yycH"
                     /locus_tag="ACG8M6_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0457322.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            11955..12824
                     /locus_tag="ACG8M6_001653"
     CDS             11955..12824
                     /locus_tag="ACG8M6_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0457323.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            12879..13688
                     /locus_tag="ACG8M6_001654"
     CDS             12879..13688
                     /locus_tag="ACG8M6_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0457324.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13791..14420)
                     /locus_tag="ACG8M6_001655"
     CDS             complement(13791..14420)
                     /locus_tag="ACG8M6_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0457325.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            14590..15882
                     /locus_tag="ACG8M6_001656"
     CDS             14590..15882
                     /locus_tag="ACG8M6_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0457326.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(15958..16620)
                     /locus_tag="ACG8M6_001657"
     CDS             complement(15958..16620)
                     /locus_tag="ACG8M6_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0457327.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            16845..17129
                     /gene="groES"
                     /locus_tag="ACG8M6_001658"
     CDS             16845..17129
                     /gene="groES"
                     /locus_tag="ACG8M6_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0457328.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            17180..18805
                     /gene="groL"
                     /locus_tag="ACG8M6_001659"
     CDS             17180..18805
                     /gene="groL"
                     /locus_tag="ACG8M6_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0457329.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6461 a         3060 c         3930 g         5527 t
ORIGIN      
        1 aaaatttttt agaaggagtt gtgttagata agtgataaaa tcatttcctt ttgtgcagca
       61 acaagatgaa aaagattgtg gtgttgcttg cctttcgatg attttaaaat attttaaaac
      121 tgatatcccg attcataaat tacgggacct atctgggact gatttagagg gtacttcagc
      181 atttggattg aaaaagacat ttgaaaaatt gtcttttgat tgtatggcta ttcaagcaga
      241 tagtgagatc tggaaagaaa gagatcttat cttaccatta attgcacata tattggttga
      301 taacagctat atgcactacg ttgtaattta taaaacgaag ggaaacgtac tatggattgc
      361 agatcccgct aatggaaaaa atagaaaaac gattgaagcg tttcaaaaag aatggacagg
      421 tattctttta ttacctatgc ctagagaaac ttatattcct attaaagaaa aagtagctgg
      481 attaagttct tttttgccta taatttggaa acaaaaagga ttagtattcc acatcgtttt
      541 agcatcgatt ttcattacgt tttttggcat aggaagctca tattattttc aaggtattct
      601 tgattttttc atccctaatc aagcacgttc aactttaaat ataatttcca ttggcttgat
      661 cgctgtctat ttatttcggg tattgtttga atatagtcgt agttatcttc ttattatttt
      721 aggacagcga atgagtatgg caattatgct tcagtatttc aaacatgtgt taactttacc
      781 aatgaatttt tttgctacga gaaaatcagg ggaaattatt tctcgttttc ttgatgccag
      841 taagattatt gatgctttag caagtgctac gttatcaata tttttagata ttggtatggt
      901 gttgcttgtt ggggttacgt tagctattca gaatagtacg ttgtttttaa tcactctatg
      961 ctctttgcct ttttatgtag ttgcaatatt cgctttcgtg aaaagctatg aaaaatcgaa
     1021 cagggaagaa atggcagccg gtgcagctgt aaattctagt atcatagaaa gtcttaaagg
     1081 aatagaaaca attaaagcat atagcggtga agagaaagta tataaccgta tagaccatga
     1141 atttgttaaa ttaatgaaaa aatcatttcg tacggttacg cttgataata ttcaacaagg
     1201 aattaaacat ttgattcaat taattagcag tgcattaatt ttatggctag gttcttattt
     1261 tgttatggat aatagaatta gtctaggtca acttatcaca tacaatgctt tactagtatt
     1321 tttgacagat cctttacaaa acattattaa tttacaagtg aaaatgcaaa cggctcaagt
     1381 tgcaaataat cgtttaaatg aaattttcgc gattgattca gaaaaatcga accagtcttt
     1441 tgaatatatg ctttctgaac aattatttca acaagatatc actgtaaaaa acgtttcatt
     1501 ctcgtatagc atgaaggccc caacgttgaa cgaaatttca tgtacaattc cttcgcacag
     1561 taaaacggct atagttggag ttagtggttc tggaaaatca actcttgcaa agttatttgt
     1621 tcgtttttat gaagcatcag aaggatcgat ccattatgga aacattaatc acttagacat
     1681 tccttttcag atattacgcg atcgagtgac atatttgcct caagagtcat ttttctttag
     1741 tggaacaatc attgagaatc ttatattcgg tttagatagc ccaccaagtt tcgaacaaat
     1801 aatagaagtt tgtgagatag tccaactaaa tgaatttatt aatcaacagc cattacgtta
     1861 cgagacattg ttggaagaag gtggaaataa tctttcagga gggcaacgac aacgattagc
     1921 cttagcacgg gctttattaa aaaatccgga catattaatt ttagatgaag caactagtgg
     1981 attagatacc ttgttagaac atgcaattat gaattatctc ctcaatttaa aagaaaaaac
     2041 gattattttt attgcgcatc atttaccaat agcaaaagca tgtgatcaaa ttttagttct
     2101 ccatgaaggg catttggtag aaaaaggaac gcacgatgaa cttcgttttg ccgatggtat
     2161 ctatcaaaaa ttatgggaaa tctaggagag ggaaacaatt tatgcagaat aataattggt
     2221 tcgaacattc tagtatctat actaaacaac atcatagctt ctaccgttgg gtgttatacc
     2281 ctgtaattct ttttttatgt attattggat tgtttttaac ctttgctaaa aaagaggttg
     2341 ttattcggac aacggctcaa ttgaaccccg aaaaaataga gaagttacaa gtgccattag
     2401 aagctaaaat aatggaaaac ttcctaaaag aaaatcaatt tgttcataag gacgatgtgt
     2461 tggttagatt ggattgttct ttaatagaga atgaaaaggc tcagatagaa caagaaaacc
     2521 aacgtattac tcaacaaata aagatggctc agctatttat tgaaagtata agtaaaggaa
     2581 aaaatttgtt ttcaacggat gacagttttg gctacagtaa tcaattaaag agcatgttgt
     2641 cagaaaaaga atcactccgc tacgctttga agcaaagtga attaaatgat caaaagcaat
     2701 tagaagtata cgaaaagaca aaaagacaac tagaaaaaca aattgagagt tcagatagta
     2761 aattacaaga atggcaacaa gtacaggtag cttggagtaa taatcaatca ttaaaagatt
     2821 tttcaaaaga aatgatggca aactatgaga attggcaaga acaactaaat aatgtttctg
     2881 atgatcaaaa aaatcaagtg aaactgacaa tttcagcaag cataaatgaa caaattgagc
     2941 aactaaaaaa agaagtagaa cagtatcagt cagaaaaagc taaattagtt aaaccaacta
     3001 cttctgagaa tgacagaatt agtcaaacgg aaaaaggaaa gcaagagcta gaacaagcaa
     3061 taacaactgt aaaacaaaca gttacagagc taaatgaaaa acaagagaag aaccagtcag
     3121 ttatcaaaag tttggacgat caactttcaa aaggaatact aaaagctcct gtatctggaa
     3181 cggttcattt aaatgaagaa acaaagggac aagcagaact agccaaagga acagtgcttg
     3241 cagaaattta tccgatccac gaaaaatcaa aaatgaaatt tacagcactt ttacctgcga
     3301 atgaaagtac ctatattaaa gaagggatga aggttcattt taaattggat caaaaaggtg
     3361 gggctccgat tacgatcgac ggaactttag atgaaatttc agaaaattct actgccactg
     3421 agaaaggtgt tttttatgca gtgaaaggaa cgttaaaaac aactcaatcg tttccgtttc
     3481 gttatggatt aactggagag ttgtctttaa ttgtaggaga aaaaacatat tggcaacaaa
     3541 ttgtcgatgt gtttctaaat aaaaaataat tatttttact aagaatatag gaggagttta
     3601 tatgaataaa agaagatgga ttgctgtggg agtagcgtgc ggattattgt tactatccat
     3661 tatcgtcgcg ctaattccag gaaaagataa agaggaagca tcaaatacaa ccctaacagg
     3721 aataaataag attttttacg gatctaacga aatcacagaa gaaacgttag aagaaggcgc
     3781 atcaaataaa aagattgtca aactatccgt caatggagtt attgcagata caggtgaaag
     3841 caatcttttt agtagggaac aatataacca tcagaatttt ttaacacagc taaagaaaat
     3901 tcaagaagac aaagcggtta agggagtgtt attagaagta aattcacctg ggggaggaat
     3961 atatgaaagt gctgagattg ctaaagaaat ggcaaaaatt aaaaagctag atattcctat
     4021 ctatactgca tttaaaaata cagcggcaag tggagggtac tacatttctg ctggttcaga
     4081 taaaatattt gctacggaag aaacaacaac tggatcaatt ggtgtcatta tttctggttt
     4141 aaattattct ggcttattag aaaaacttgg cgtaactgat gcgacgtata aaagtggtgc
     4201 attaaaagat atgatgtcac ctcaacacaa accttcagag gaagagaata aagttataca
     4261 ggaatttgtg atgagtgctt atgatcgttt tgtcaatgtt gtagcaaaag gaagaaatat
     4321 ggatactaat gcagtgaaag aacttgcgga tggaagaatt tatgatggta accaagcagt
     4381 agaaaatggg ttggtggatc aaattggtta ctctgaagat gcattagatt ccctaaaaaa
     4441 agagaagaaa ttaactgatg cgacggttat agaatataaa aatgacacaa ctggctttgc
     4501 ttcttcttgg ttaggtaata agattgctga atggcaaggg ctaaaggcaa ctacatcaga
     4561 tcgtgtaatt tcagtttttg aaaaacttgg attttctgat actccaaaac caatgtatta
     4621 ctatggaggg ctataaaatg aataagaaga gtggcattga aactttgcct aattatgtat
     4681 ttgcgggatt ttggttacga ttttttgctt ttcttattga tttaatttgt attagcatca
     4741 tcacccatgc tactataggt ctcttttaca aaatcatggg actggaatct gacaaatcca
     4801 ttattacttt ttatggtata gcgtcattag ctatttattt aacctatttt gtacttttga
     4861 ctaagctgaa ttcaggacaa acaattggga aaatgatttt tgggataaga gtagtgagct
     4921 ttgaggaaaa agagctaagt tggcaaacta tctttattcg tgagtgtata tgtaggttta
     4981 tattaaaaaa ttttgtattt caaattgggt atttggtggc tgctttcacg cccaagaaac
     5041 aacatattgg ggattatttt tctaacacgt cagtggttgt tattcgttca ttagaagcta
     5101 ttaactatgg tcaaaaaatt tctaattcta aaacacttaa tctggagaga attaaatgaa
     5161 taagagaaaa acagagttta ttttgatttt aattagtgga ataacaggga ttttatgtag
     5221 tcttttagca ttgatgggtg gtatgtttct atcttctatt gattcaacta ctttaaatac
     5281 tacagaacta accagtaagg atatagatat gttagtcaat ctaagcggga atgttgtttt
     5341 atttggagga attaccctag ttttttcgat aatgattttc ataataggtt ttttagtaag
     5401 aaaaaatcaa aaaacagtgg tctatggtat tagtttactc attttatcta ttattccatt
     5461 ttttctttta acattattgt gggtaattcc tggagtttta ggtattattg cgggattgat
     5521 gcttctaata agaaaatctt aattaggcaa tgtgtagaca acgtgaaacg attaatcaat
     5581 taaaagctaa atacgttttg agaaatgata acttgtagtc gatggaagac atgtagataa
     5641 aaaaactacg tgtcttttat ttattctaat tttttcgaaa aaaagacact ctctgattgt
     5701 tgtattaatg gaaaagggga aagcagcaag taaatttagt ttttctatta aatattcttc
     5761 actttttaac cttttatagt acttagattt aatcctatat gtttagagag ttcattggca
     5821 gcatgatttt gcaaagcttc tcgaattatt gttatattag aaactttact gttcataatt
     5881 atgcttcttt tttaactcaa agtccaaagg gctacagtgg actttgagtt aaaaataaaa
     5941 agccctagtt attttgaaaa ataaaaaaag aacagcgtaa atgttttcga aatgctttcg
     6001 caccaacatt tacctgttcc gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc
     6061 gcgacccgct gattaagagt cagctgctct accaactgag ctaacggccc gtacaaaaag
     6121 tacttaataa tagtagcact aagaatttta aaatgcaaga ctacatttaa agaaaatcag
     6181 aaaaatcttt acttccttat aggaaatcat tcatagatta ttcacatttg ctcgctatta
     6241 tataagcata ccaacaaaca aagttcttgc aagagaacaa accctaacac ttttttcatt
     6301 ttttactcct ttcccgccgt ctccccggcg ggtctcctgt tcttaacgat aaaagacaag
     6361 aaggcttagc cttcttgttt tttgttttca tggaatcccg ctaatcaaat cacctagtta
     6421 aagcaactag attgaatgtt tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg
     6481 agcggttatg atggaaaaaa tgcgtcagca attaatggag tgggggaagg aactagaaac
     6541 attccgtctg cctcactggg aagaactccc tgacttagaa ctttatatgg accaagtcag
     6601 aacgttagtt gatcgttatc tttcacctgt gatacaagga gaaaagcatc cattgcttac
     6661 ctcttctatg gtaaacaatt acgttaaact aggtttgata ccagcaccag ttaaaaaaag
     6721 atataacaaa gaacacgtag catttttact tgcaatcact acgttgaaac aagtattgac
     6781 tattcctgaa atcaaagaag ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc
     6841 ctataatcta ttttgcgatg aacaagaagc agctgtgtgg atggtcagcc aattagcaca
     6901 aggtaaatct catccgcaaa aattcgacca ggcaacccct gtagaatata ttgctgtaag
     6961 agcggcaact ttatcgtttg caatgaaact tcttgcggaa aaaacgattg ttctagaaac
     7021 cgaatattta aaagaggaga aaacaaatga aaaacaataa aatcgcaatt ttagtagatt
     7081 caggtacgga tgttccagaa gaattgataa aaaaatacga gatgtatatg atcccattga
     7141 aaattattta taaagatcag gtatatacag ataaaattga cattacccct gaagagatct
     7201 atcaacgttt gccagaggaa atccctggta catctttgcc ggatggtgag tcgatcacag
     7261 aaatctttga gcaaatcaaa aaagacggtt atgaaaaggt acttgcggta acaatttcca
     7321 gtggattgag cgggacatat aatgtggttc gtttaattgc acaacagcaa gaagaattgg
     7381 aagcatatgt actagatacg aaaaatattg gtattggtgc aggtttttct gcgatccaag
     7441 cagcaaaatg gctggaagaa ggagtagaat gggatcaact gatcagtaat ctgaatgagt
     7501 tagtcgaaag aacaaaagtt ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg
     7561 gacggatcgg tttagttgct tctattgtag gaaccgcttt aaagcttaac ccaatcatct
     7621 catgtaatga agaagggatt tattacacag taggaaaagc taggggacgt aaaaaaagtt
     7681 tggataaaac ggttgcttac gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag
     7741 ctgttgccca tggggatgcg aaagaagaag caattgaaat ggaagcgaga cttaaaagag
     7801 agttcccaca agcaaaccaa atctactttg gccagatttc tccggcatta gtagtccaca
     7861 ctggtccggg attattgggt gtcggtgtac aagttttaga aggataaatc aaaaaaagga
     7921 aacactgagg tgaaaatcag tgttttcctt ttttattgaa gttagaacaa aaaataataa
     7981 agtattttgc actttcattt taattttctc ttttgtttta taggcaaaga tgaaacaaaa
     8041 gcagaaatac ggtataatga gaaggtatga caataatata taagaggtgg ctctttatga
     8101 aaaaagtttt agttgtcgat gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag
     8161 ccaaagaagg ctatgacgtt tatactgcat atgatgggga agaagccttg gaaaaagttg
     8221 ctgaggtaga acctgatttg attttacttg atttgatgct acctaaaatg gacggcttag
     8281 aagttgctcg tgaggtacgc aaaacttatg atatgccgat cattatggta acagccaaag
     8341 attctgaaat tgataaagtc ctaggcttag agttaggcgc cgatgattac gtgaccaagc
     8401 cattttccaa ccgagaatta gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg
     8461 ctgcgaagga accagaagag gcagcacctg cggaattgac gattggtgat ttgacgatcc
     8521 atccagaagc ttacatggtg acgaaacgag gagaaacaat cgagttaaca catcgtgaat
     8581 tcgaactact cttttacttg gcgaagcacc ttggacaagt gatgacaaga gaacatttat
     8641 tacaaacggt ttggggttat gattattttg gagatgtacg aacagtggat gtaactgtac
     8701 gtcgtttgcg tgaaaagatc gaagataatc caagccatcc aaattattta gtgacacgtc
     8761 gtggtgtcgg ttactatttg cgtaacccag aacaggagta attggatgaa aagaaaagtc
     8821 cgcttttttc gatcagtcaa ttttaagatc gctattacct ttattctgat tttattgatc
     8881 tcgatcgaaa ttattggtgc ctactttata cgtggtttag aacgttccac gatcaataca
     8941 tttattaaag atatgaatca aacggtggaa tcattagcaa caacaatcag cccggaattg
     9001 aatcgaaaag ataatgcaga tgatgaagag gtaaatgcga atatcaaaag atttattgaa
     9061 aacagtgcga ctagcgatat catcgaaata cgggtcgttg acgaaaaagg gatcatccga
     9121 ggaacaaccg atgtcaatga acaaagcgct gtgggaaaga aaaatgatta tgttgatatc
     9181 aatgatttca cgactaaacg ttatgttgct ttagataacg acgataaacg ggtgaatatc
     9241 aatgttcaac cgatcctgtc cccgaccggc gatgccgtta tcggggctct ttacgtcaaa
     9301 agtaatatcg aacaaaaata ttcggaaatc aataacacag cagtcatttt ctttactgcc
     9361 tcactaattg cggcatttat ttcaatgatc gtatctgttt tagttgctcg ttcgattaca
     9421 cagccgatcg gtgaaatgag agaacaagcc attcgaatcg ctagaggaga ctacagtcgg
     9481 aaagtcaaag tgcatggaca agatgaactt ggacagctgg ctgatacctt caatcagtta
     9541 gctgaacgta ttgaagaagc tcaagataca atggaagctg aacgcaatcg tttggatagc
     9601 gtgctatccc atatgacaga cggcgtcatt gcgacagatc gcaggggtaa agtcattacg
     9661 atcaatgata tggctgtttc cttgctagat atcaaaaacg aggaagcaat cggacaatcg
     9721 attttgacac ttttagatat cgaagaagaa tatacgttgc ggaaattatt agaagatcca
     9781 aacgaaatgg tacttgaccg ttcaaccagt acattggaaa gtgatcagat gatcttgcga
     9841 gtagactttt ctatgatccg tcgcgaatca ggttttatca gcggattagt agctgtcttg
     9901 catgatgtga cagaacaaga aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt
     9961 tcccatgaac ttcgtacacc gttgacaagt atgcgtagtt atatcgaagc attgagcgaa
    10021 ggtgcgtgga aagacgagga agtagcacca aattttttga aggtaacgct agatgaaaca
    10081 gaccgaatga ttcgaatgat caatgacctc ttaaatctat ctcgcatgga tacaggtaat
    10141 acccagcttc aattagaata tgtcaatttc aacgagttag tgaattttgt tcttgaccgg
    10201 tttgatatga tggtgggaaa tcaggaaaaa aattataaga tccgtcgtga atttacgcaa
    10261 agagatcttt gggtcgaagt agatacagat aaaatcatcc aagtagtcga taatatcatg
    10321 aataacgcga tcaagtattc accagatggg ggaacgatta cttgtcgctt attagaaacg
    10381 cataataacg tgattttaag tatcaccgat caaggattgg ggattcctaa aaaagatctg
    10441 aaccgagtgt tcgagcggtt ctatcgagta gataaagcac gtgcaagagc acaaggtggg
    10501 acaggacttg gattagctat ttctagagaa gtgattaagg cgcatcgagg cgctatttgg
    10561 gcagagagca aggaaggaaa agggtcgact ttttatatct cgctgccata tgaaccttat
    10621 gaggaggaat ggtgggaatg agaatctctg aaaaagtcgt tcgtatagga ctcattttga
    10681 tggttgcgtt aagcatatac ttctcttatg caatctggct gagcccagca ggaaaaagtt
    10741 caatcaacgt agatgagaca aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt
    10801 ccgaagtctt tcttccgctt catgttacat ggcaccattc gggaatgatc caagaaacca
    10861 acagtgaaaa tctcgtgtac cgattgcaat tgatcatcga taaagcaaga tttggaaagc
    10921 tttctgaagt agtttcagga gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg
    10981 gttttgagtt ggcctataat gcgccttttt tgctaagaga atacaaggaa gcatttgatt
    11041 tagatttaga tgtgtcgaac ttggcaaaag aaagcgaaaa tatgtatttt actagaatac
    11101 aattcgatca aggaaataaa aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg
    11161 aagcaaatat ttcgttgaat tggacagata tcaataaaga attaacagca aaagacacaa
    11221 aatggacaga aatgacaaca gaaccatcga cgatcgacac gcaatatgct acgaaacatt
    11281 cgatcaaact gaagaagtat agttatatcc tgtcacagca accctataca ctttttagaa
    11341 atgctttttt ccagaaacca gatagcgtga aaaataatga tgaaagcaat gaattgattt
    11401 tttacggtgg gaatgaaaca atgtctatcc attcggaaac gcaagtagtt gattttcgtg
    11461 gagagctgcc agagaaaaaa gaaaatgcta gtgtattttc tgaaagtttc ccctatatta
    11521 gcaaattagg cagttcttta gggaacctca gatattttga ccgcagtgac aatacgatcg
    11581 attatcggat attcgtggaa ggcttcccgg tttttgggac agacagcaga ggacagattg
    11641 gtgtggaagt tggcggtgaa acgaatggtt ctgtgcaagt gaatatccaa accagtttga
    11701 atacgattca agtgccgata ccttcagacg aagaagtaga acttgcaagt acgaatgaaa
    11761 tcatccagca gttaaaagct aagggagcag atagcaacaa gattggttca gtgatcgttg
    11821 gctatacatg gcaaaatgtc aaagaaacga atcgtgtcgt tgatctctta cctgaatggt
    11881 atgtgaaata taaagataac tggtattcag ctaacgacct tctggaaaaa ttacctggat
    11941 tggaggcgaa ttaattggat ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag
    12001 ggttgaatgt gtttttattc agcagttatc atgacgcggt ataccaagaa aacaatgtca
    12061 ttcgctctaa tcagactgaa tcaatccaac aacgtctggc tagcgacgat atcacttaca
    12121 cggggaagat ttcgtctgag aacaagcaag gctattatct tagcgcggag cagaccaatc
    12181 ttagcacttc attagcgaat tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg
    12241 tgtttttaga cggtgatgtt ttgacgaaat ctttatccag ttcagatgct gaaaagaacg
    12301 tgatcgatcc agataaaatt agtagttcct tggaagattt tttatctaac aaagacaata
    12361 ttttattcgg taatgattat gtgtacacga aaaacttttc tcatttagaa gaagaaccac
    12421 tagaaatcca agcaagccaa gagtatgaag gcatcccatt caatgatgat acagcaaaaa
    12481 tcgttctttc tttagaaaaa aacgataatg attatagcat cacgaaatac atgcagacgc
    12541 atttatctga tatcgagcag ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa
    12601 tcaatacatt gtacatcaat aacaaaatac caagaggatc aaaaatttta tggcgtcagc
    12661 tagcttattc ctgtattctg aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg
    12721 tagcgatcga aacaagcgat aaagtggtcc aaattgagag cgtcaacgct ttcagcaata
    12781 cgatcgtgac aaacaatact gtaccaaaag tggaagatca ataaaaaagc gagtataatg
    12841 aaaaaagttt acaaagtaag aagggaagta ggagtcaaat ggctgaagaa aaaccagctt
    12901 ttaaaatcag tatcctagca agtggaagtt cgggtaattc actttatatc gaaagtgaaa
    12961 aaaaacggct tttggtcgat gcaggactta gcggtaaaaa aatcacttcc ttgatggctc
    13021 aaattgatcg gacaccggac caattagatg gaatacttgt gacgcatgaa catcgagatc
    13081 atatacatgg cgtgggtgtc ttggctcgta agtatcattt ggatgttttt gccaatcagc
    13141 agacttggga agcgatggaa ccactgattg gaaatgtacc agtggaacaa aaacatctat
    13201 ttgagatggg gaaagtcaga acattcggcg atatcgatat tgagagtttc ggcgtctccc
    13261 atgacgcagc ggctccgcaa ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt
    13321 tgacggacac cggatattgc agtgattata tgcgaggtgt cattgaaaat gccgatggtt
    13381 atttgatgga aagtaatcat gatcttgaga tgttacgcat gggaccttat ccgtggaatt
    13441 taaaacagcg tattttagga gatagaggac atctttccaa cgaagacggt gcacttgtca
    13501 tgactgatgt gatcggtaat cggaccaaac gaatctatct tggccatctt agcaaggaaa
    13561 acaatatgaa agaacttgct catttaacaa tggaaaatgt tttgaaagaa aaagattttg
    13621 gcgttggaca tgattttgaa atctatgata cagatccaga tgaagcaact gagctatttt
    13681 cgatttaaac agttcaatca ataaaaatat aaaaagcaga agataaaatc aatcagggtt
    13741 ggttttatct tctattttta tttttttagc atattaaaaa aataaccgta ttatcgactg
    13801 actacactga tgtgctgctt ttcatgtttc tcatccagta tctcatctac taatgcaatc
    13861 gcgtaatctg ccatgctgat cgtactgtcg ctagcagcgt ttctttgtaa ataatcatca
    13921 cttaacaaat aagtacctgt acgtttgcca tgtggatcaa ataaagaagc tggactgata
    13981 taagtccaat gtagattttt agattgtttc aattttaaca atccttcgta catattgaaa
    14041 gctgttggat agaagggtgc atttttaggt acttggtcaa tcatacgagt cgtcttttct
    14101 gtatctaaga ataacgaaga ggcaccccca acgacaatta agcgtgtatc ggttccttca
    14161 agcatctcaa tcaaatggga caaagaagtt tgatgcattt cttcttttcc tttgggagcg
    14221 ttgaaagcat caacgacaac atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa
    14281 tccttttgta taaacggaac atctgatggc aatttttctg gatgacgcac gattgccgtc
    14341 acgtctaaat ctctggacaa tgcttcgtct aaaataaatg aacctgcatg gccagtagct
    14401 ccaatgagtg caattttcat aaaaaacctc cttttttatt cctgtccatt gtaagcagtt
    14461 gctgaatatt cgtcaaagaa tccgattttt gaaaagctca gtgcgcattc ctttcatggt
    14521 tcgtgatcat tgggtatagt taaattaaga aaactgattg ataaaaatag aagttgaagg
    14581 agataagaaa tgaaagagtg gttagatcgt gcctttttac caatgataac atctgttgtg
    14641 aatcttagac ccttaactgc tttgcgtaat ggaatgactt acacgatccc aataattatt
    14701 gttggctcgc tgtttttctt atttgctcat tttcctgtgg attacatcgc tcagtggata
    14761 gacaaaacag gattttctat gtattttaca caagtatacc aggctacatt tgatgtgata
    14821 gccttttgga tcgtttttgc cattgcatac gcttatgcaa aagaagaaga catcgatcgc
    14881 ttaccagtcg gcataacagc cttgtctgct tatctactgc ttattcagcc actaaatact
    14941 cagatagata ctgtctggac aggtgctgaa gggatactag gagcagttac aactgggctc
    15001 atcatcggat ggggctatgc acgtttagta agacttgcga aagggtggaa gcaaagagag
    15061 aagatacctg aaaatgtttg gcagtctttt acttcattga ttccaatcat tgttatagtc
    15121 acttttacgc ttatcggttc agcggttttt caacatttgt tcagaatgac tgttgttcaa
    15181 ggagtatgga catatgtcca gcttcccatt caatcgttca tagacacgtt gtttggtgtg
    15241 cttttattag gtttttttgt tccgttttta tggttatttg gtcttcatgg atctgcgatg
    15301 gtcaatgggt tggttagtcc cgtcttgcaa gcgaattcat tagcaaatgc agaaatctta
    15361 gcctcaggac aagagttgac ggtggcaaat ggcggacata tcgttactca gcaatttctc
    15421 gatcagttca tgacggttac gggtgctgga ttgacgttag gggctgtatt ttttatgacg
    15481 gtttttgcga aatcgaagaa atacagagaa ttaggaaatc ttgctctttt accagccttt
    15541 ttcaacatca atgaatcaat tattttttcc acaccgatcg tgatgaaccc gatgatggct
    15601 gttcctttta ttttcgcacc ggtcgtttca ggattgatta cctattccgc tttatatttt
    15661 ggattcgttc ctttatttac agcagtccaa gtcccttgga caacaccgcc gatcctctcg
    15721 ggtttcttag taggcggagt gcctgcagcg attttacaag gtatcgtgtt gtcaattagt
    15781 tttttcattt atttcccatt tttgaaaaaa atagattctg caaactgcaa aaaggaaaga
    15841 cagacaaaaa atgatggagc agcagaaaag atgaacgact aaaaaacaaa aatactcctg
    15901 tcgctttaga ttgggaaaat caccaatcta ggatgacagg agtatttata gtcatgttta
    15961 tactagccct ttttggacag ctaattgaac aagaacaact aatgtgttca ttcctacgtg
    16021 tgtgatcatc gatgtccaga tccgaccagt tgctttgtac tctaggctga agaagaaacc
    16081 taggaaaaag tagattagca gatgaccatc attatgagca aacgcaaaca gcaaagagct
    16141 gatcactgct ccgacccaaa aatttgaata tcgactgatg atccccagta tactgcggcg
    16201 gaatacgaac tcttccatga tgggtccagc aacagttgtc gccaaaataa aagcgggtgc
    16261 ttccatgatc atctggatga tattttgtgt attctcggaa ggggttgctt tgccaaacaa
    16321 tacagattct aacatgacag cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa
    16381 tccataaata acaatctttg gagcagaagc tttttttacc gctgattcga tcgctaaggg
    16441 ttcacgttgc ttaaaataaa aaatagctag gacaacggcg cctagtatat acatgactgc
    16501 agttgctgtg atcaattcag acgtagattt tacaagtcct atcgaagcta ggatcaaggg
    16561 tgagataaaa attagaccat agcaaaaaat gctgaggaag ctatattttt ttaaagacat
    16621 gtaattcctc cttttaaggg ataacaagca tgcaaggcaa gcttatatcg attgatttca
    16681 attatatcat aatttcaata agcagctgtc agaataaaat ttcttttctt tgatttaaaa
    16741 gcttgcaaaa aagagggaga atgagtatta ttaaaaatgt aggttagcac tcgaaggtta
    16801 agagtgctaa cagattaaaa aaagagttat cggagggatt tatcgtgtta aaaccattag
    16861 gtgatcgcgt cattatcgaa gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt
    16921 tagcttctgc tgctaaagag aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc
    16981 gtttgttaga aaacggtgaa aaagttcctg ccgctgtaaa agcaggcgac caagttatgt
    17041 tcgaaaaata cgccggtaca gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag
    17101 gaaaagatat catggcgatt gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa
    17161 tcaatgaggt gaatcaatca tggcaaaaga attgaaattt gcagaagacg cacgcgcagc
    17221 tatgttacgc ggggtagata aattagcaga tacagtgaaa gtaacattag gacctaaagg
    17281 ccgaaatgtc gtactagaaa aatcttacgg ctctccattg atcacaaatg atggtgtgac
    17341 gatcgcaaaa gaaatcgaat tagaagatca ttttgaaaac atgggtgcaa aacttgtatc
    17401 agaagtagca tcaaaaacaa atgatattgc aggagacggt acaacgacag caactgttct
    17461 gacacaagca atcgttcgcg aaggtttaaa aaacgtaact gcaggtgcta atcctctagg
    17521 cattcgtcgc gggatcgaat tggcaacaaa agcagcagtc gaagaattgc ataatatctc
    17581 aactgttgtt gattcaaaag aagccattgc acaagtagca gctgtttctt cgggttctga
    17641 taaagttgga catttgattg ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac
    17701 aatcgaagaa tcaaaaggga tcgaaacaga actagatgtt gtagaaggta tgcagtttga
    17761 tcgtggttac ttatcacaat atatggtaac agataacgac aaaatggaag ctgttctaga
    17821 aaacccatat atcttgatca cagacaaaaa aatctctaac atccaagata ttctgccatt
    17881 gttagaacaa atcttgcaac aatcacgtcc attgttgatt attgctgacg atgtagatgg
    17941 tgaagcattg ccaactcttg tattgaacaa aatccgtgga acattcaacg tagtagctgt
    18001 aaaagctcct ggtttcggcg atcgtcgtaa agcaatgctt gaagatatcg ctattttgac
    18061 aggtggtaca gtaattactg acgatctagg tttagaattg aaagatgcaa caatcgaaaa
    18121 cttaggaaat gcttctaaag ttgttgtaga taaagacaat acaacgatcg tagaaggttc
    18181 tggagaaaaa gaagcaatcg aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac
    18241 aacttctgat tttgaccgcg aaaaattaca agaacgctta gctaaattag caggtggtgt
    18301 agctgttgtt aaagttggtg cagctactga aacagaattg aaagaattaa aattacgcat
    18361 tgaagatgca ttgaatgcta cacgtgccgc agtagaagaa ggaatggtct caggtggtgg
    18421 tacagctcta gtgaacgtta tcagcaaagt ttctgctgta gaagcagaag gagacgtggc
    18481 aacaggtatc aagatcgttg ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa
    18541 cgctggttat gaaggatcag tgattgttga taaattgaag aacgtggaac taggcactgg
    18601 tttcaatgct gctactggag aatgggtaaa catggttgaa gcaggtatcg tagacccaac
    18661 taaagtaaca cgttctgcct tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac
    18721 agaagcagtc gttgcagata aaccagaacc agcagcgcca gcagctcctg caatggatcc
    18781 atcaatgatg ggcggtatga tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta
    18841 gtgaaagaca gataaaaaga ggctggaaca gaagcgttta actccaagaa ataagaagaa
    18901 attcacggaa attgcttttc aaatttttgt gaatttcagc ttatttccga aggagttgct
    18961 tctgcttccg ctgtttat
//
LOCUS       DAZKOB010000050        18872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000050 DAZKOB010000000
VERSION     DAZKOB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.050"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..582)
                     /locus_tag="ACG8M6_001660"
     CDS             complement(7..582)
                     /locus_tag="ACG8M6_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0457330.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(587..1561)
                     /locus_tag="ACG8M6_001661"
     CDS             complement(587..1561)
                     /locus_tag="ACG8M6_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0457331.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1585..1629)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1844..2812
                     /locus_tag="ACG8M6_001662"
     CDS             1844..2812
                     /locus_tag="ACG8M6_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0457332.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2925..3203
                     /locus_tag="ACG8M6_001663"
     CDS             2925..3203
                     /locus_tag="ACG8M6_001663"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0457333.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            3321..3557
                     /gene="secG"
                     /locus_tag="ACG8M6_001664"
     CDS             3321..3557
                     /gene="secG"
                     /locus_tag="ACG8M6_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0457334.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            3660..4415
                     /locus_tag="ACG8M6_001665"
     CDS             3660..4415
                     /locus_tag="ACG8M6_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0457335.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4418..6775
                     /gene="rnr"
                     /locus_tag="ACG8M6_001666"
     CDS             4418..6775
                     /gene="rnr"
                     /locus_tag="ACG8M6_001666"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0457336.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6797..7261
                     /gene="smpB"
                     /locus_tag="ACG8M6_001667"
     CDS             6797..7261
                     /gene="smpB"
                     /locus_tag="ACG8M6_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0457337.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7563..8282
                     /gene="atpB"
                     /locus_tag="ACG8M6_001668"
     CDS             7563..8282
                     /gene="atpB"
                     /locus_tag="ACG8M6_001668"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0457338.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8335..8550
                     /gene="atpE"
                     /locus_tag="ACG8M6_001669"
     CDS             8335..8550
                     /gene="atpE"
                     /locus_tag="ACG8M6_001669"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0457339.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8641..9165
                     /gene="atpF"
                     /locus_tag="ACG8M6_001670"
     CDS             8641..9165
                     /gene="atpF"
                     /locus_tag="ACG8M6_001670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0457340.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9152..9694
                     /locus_tag="ACG8M6_001671"
     CDS             9152..9694
                     /locus_tag="ACG8M6_001671"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0457341.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9717..11273
                     /gene="atpA"
                     /locus_tag="ACG8M6_001672"
     CDS             9717..11273
                     /gene="atpA"
                     /locus_tag="ACG8M6_001672"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0457342.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            11287..12189
                     /locus_tag="ACG8M6_001673"
     CDS             11287..12189
                     /locus_tag="ACG8M6_001673"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0457343.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12212..13618
                     /gene="atpD"
                     /locus_tag="ACG8M6_001674"
     CDS             12212..13618
                     /gene="atpD"
                     /locus_tag="ACG8M6_001674"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0457344.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13632..14054
                     /locus_tag="ACG8M6_001675"
     CDS             13632..14054
                     /locus_tag="ACG8M6_001675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0457345.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14523..14753
                     /locus_tag="ACG8M6_001676"
     CDS             14523..14753
                     /locus_tag="ACG8M6_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0457346.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14850..16151
                     /gene="murA"
                     /locus_tag="ACG8M6_001677"
     CDS             14850..16151
                     /gene="murA"
                     /locus_tag="ACG8M6_001677"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0457347.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16164..16340
                     /locus_tag="ACG8M6_001678"
     CDS             16164..16340
                     /locus_tag="ACG8M6_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0457348.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16475..16771
                     /gene="yidD"
                     /locus_tag="ACG8M6_001679"
     CDS             16475..16771
                     /gene="yidD"
                     /locus_tag="ACG8M6_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0457349.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16835..17218
                     /locus_tag="ACG8M6_001680"
     CDS             16835..17218
                     /locus_tag="ACG8M6_001680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0457350.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            17387..18271
                     /locus_tag="ACG8M6_001681"
     CDS             17387..18271
                     /locus_tag="ACG8M6_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0457351.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            18385..18621
                     /locus_tag="ACG8M6_001682"
     CDS             18385..18621
                     /locus_tag="ACG8M6_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0457352.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18709..18789
                     /locus_tag="ACG8M6_001683"
     tRNA            18709..18789
                     /locus_tag="ACG8M6_001683"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18743..18745,aa:Tyr,seq:gta)
     gene            18796..18867
                     /locus_tag="ACG8M6_001684"
     tRNA            18796..18867
                     /locus_tag="ACG8M6_001684"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18828..18830,aa:Gln,seq:ttg)
BASE COUNT         6250 a         3296 c         4109 g         5217 t
ORIGIN      
        1 gtttattcag cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac
       61 taatgaaaca acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa
      121 gatcacactt gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga
      181 tcctgaggag gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc
      241 aggtaccgac aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag
      301 atatcttggc aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata
      361 cagagaaaaa atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat
      421 tccaggtaca ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat
      481 tggcaaacga agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc
      541 catcactgta agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta
      601 acctttccaa atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact
      661 ttcggaaaaa aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc
      721 aaaattcata acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag
      781 tgaagctttt aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat
      841 cattgatgac acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag
      901 tgatcgcttt tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag
      961 tcggtgtcaa gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga
     1021 gcttttcgaa tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg
     1081 gtgaacagtt accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt
     1141 tcccaacaga tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa
     1201 tgactgatgt atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg
     1261 attctgcctg tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat
     1321 gagtgtcttg cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag
     1381 ggatatctaa acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta
     1441 cagggacatt cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag
     1501 ctagtgataa agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca
     1561 ttatttccaa tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac
     1621 cagtccaaga cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg
     1681 aatgtaaatg ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat
     1741 gaaaaagaaa tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt
     1801 ataataaata gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt
     1861 cattcttatt ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa
     1921 gaaattagta agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg
     1981 cttgatggat cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga
     2041 gtgtgaaagc ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa
     2101 actagctgga cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca
     2161 tgattatcga agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa
     2221 aggattcaac gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat
     2281 cggaatggga tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga
     2341 acaaccagat gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat
     2401 ggcaagtggt gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt
     2461 ttctgtttat gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat
     2521 gatcatgaga tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc
     2581 atcagctaca agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag
     2641 agatcatttt gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa
     2701 agcattactg cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac
     2761 ctattggaca gtgatttttt catttattga acaaagaata acgatggatt gattttctct
     2821 tcctttttgt aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat
     2881 aaggtaggaa taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg
     2941 aaaaaaatcg tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt
     3001 cggatgcggg gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg
     3061 gtcaaaaatt agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga
     3121 aaaagattat cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc
     3181 atgacgtctt acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt
     3241 tccatttata tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct
     3301 tttgggaagg aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga
     3361 ttgttatggt cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat
     3421 ttaccggcgg tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca
     3481 tgcagcgatc aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat
     3541 ttttatcttc gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg
     3601 caaagagaac gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg
     3661 tgaaacgagt gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt
     3721 tgctgcttca tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag
     3781 aaaaagcaga ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat
     3841 atgatatctt gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt
     3901 ttttacagtc tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct
     3961 ttgctgtacg ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc
     4021 tgtctcctgt agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga
     4081 agatagcagg caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag
     4141 ctgaacatca attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag
     4201 atatccaaca gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg
     4261 gtgtatttga aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg
     4321 aaagcggtca tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg
     4381 aatttttaga aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga
     4441 gaaaattctc tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc
     4501 tgaaggtcta ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca
     4561 aatggaaaga gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa
     4621 accgatcttg gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat
     4681 tgatcccgaa gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg
     4741 ggataccgta gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga
     4801 gggaaaagta gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc
     4861 ttattcagac gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag
     4921 aaagcttaac caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag
     4981 tattgtattg atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg
     5041 gataatcaag caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt
     5101 cgtggctaac ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt
     5161 tccagaccag atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat
     5221 cgttacgatt gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt
     5281 agaaaatggt aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca
     5341 aggcagtcaa ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg
     5401 agtgattccg atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt
     5461 tccacgactc accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca
     5521 tgagattttt ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca
     5581 aatcttagaa gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt
     5641 ccaagaaatg ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat
     5701 ttcttttgag gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat
     5761 ccagctaaga agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa
     5821 cgaaacggta gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga
     5881 acagccaaaa gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct
     5941 ggtccgagga acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt
     6001 agaagacaaa ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc
     6061 acgctattca gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt
     6121 tacatctcct attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag
     6181 tgaagataaa agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga
     6241 tcatagttct aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa
     6301 gaaagcagaa ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt
     6361 tgtgaaattc ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa
     6421 tgaattgaaa caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag
     6481 aactggtctg acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc
     6541 agatgaacga gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg
     6601 tccaaaagca cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc
     6661 agaaaaaaat ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa
     6721 aaaagggaaa aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga
     6781 gttggaggaa acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc
     6841 tcgccatgat tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga
     6901 gatcaagtcg atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa
     6961 cggggaagct ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa
     7021 tcatgatccg ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga
     7081 aaacgaattg aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg
     7141 ctatgccaag gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga
     7201 tttgaaacga aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata
     7261 atttcattat cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt
     7321 tagcatttaa tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt
     7381 gcatggggaa aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa
     7441 aatgttttga attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga
     7501 aaaaaccaga tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg
     7561 gattggatga acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt
     7621 gtataatggt gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa
     7681 atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc
     7741 gaggaatcgt aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat
     7801 ttacattgtt tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct
     7861 tacctaacga aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt
     7921 tggcatttat aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag
     7981 gatatttcgt aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg
     8041 aagaatttac aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg
     8101 aagttttgct aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat
     8161 tagcaattcc attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag
     8221 catttgtctt tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat
     8281 aatactcggt taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat
     8341 tatatcgcag cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt
     8401 caagttatct ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc
     8461 cgtacaacaa tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt
     8521 atcgccttga ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc
     8581 gtttgttact gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct
     8641 atgctgaatc aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt
     8701 gtcagcggct catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc
     8761 agcgatattt tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa
     8821 tctcgcatca actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt
     8881 tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat
     8941 attttgaagg atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc
     9001 actgtagaac gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt
     9061 caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc
     9121 aatcaataca ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag
     9181 acgttacggg aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta
     9241 tcaagatctc gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt
     9301 aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg
     9361 ttatgatgga atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga
     9421 ccttttgttc atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt
     9481 gggaagtgtc acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa
     9541 tgttgcaaaa acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat
     9601 tattggtggc gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca
     9661 attagaaaaa atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg
     9721 ccatcaaagc agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg
     9781 tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac
     9841 atggtttaga aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa
     9901 tggcgcaaaa cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca
     9961 ttcgtgaagg agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg
    10021 ccttgatcgg tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg
    10081 ttactgacaa agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg
    10141 ttaacgaacc aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg
    10201 gacaacgtga attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata
    10261 cgatcatcaa ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag
    10321 attctacagt tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa
    10381 tcgttgtgaa tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg
    10441 gtactgcaat gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg
    10501 atctttcaaa acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag
    10561 gtcgtgaagc ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag
    10621 caaaattaag tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac
    10681 aagctggaga tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa
    10741 tcttcttaga aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat
    10801 ctgtctctcg tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt
    10861 tacgtctaga cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt
    10921 tggatgcggc aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac
    10981 aaaaactgca tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac
    11041 atggattctt ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg
    11101 aatatctgga taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc
    11161 cagaagaaga aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa
    11221 aaggaaataa ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg
    11281 agatgaatgg gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg
    11341 agccaaatca cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct
    11401 tcttcacaga agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact
    11461 gctactcagt taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc
    11521 atgctgctca gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt
    11581 ctggtcggtg gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac
    11641 caccaatcac ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt
    11701 aaagcacgcg gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt
    11761 gatgaagtcc gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat
    11821 gaattatacg tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa
    11881 aaaatgctgc caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat
    11941 atttttgaac cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt
    12001 ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca
    12061 atgaaaacag caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac
    12121 cgtgcaagac aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca
    12181 ctagaatagt attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat
    12241 cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc
    12301 attagttgtt tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga
    12361 attaggggac ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg
    12421 aatggaagtc attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg
    12481 tcgcgtattc aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc
    12541 ggaaagaagc gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga
    12601 aattttggaa acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa
    12661 agtcggacta ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca
    12721 taatatcgcc caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg
    12781 tgaaggtaat gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat
    12841 ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt
    12901 gacgattgcg gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa
    12961 cattttccgt ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc
    13021 tgcggttggt tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac
    13081 atctacgaaa aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta
    13141 cactgacccg gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg
    13201 taaattaaca gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc
    13261 tttagcacct gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt
    13321 attgcaacga tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc
    13381 cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa
    13441 ctttaacgtg gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac
    13501 agtgaaagga ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt
    13561 ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa
    13621 aggggtgccc catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct
    13681 atgatcacca tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc
    13741 ctaaacatgc accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag
    13801 attcagatac ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata
    13861 atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg
    13921 cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata
    13981 cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat
    14041 ctaagcactc ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg
    14101 ttatgacaga agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa
    14161 atttttgtga aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag
    14221 aggttgtgaa atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc
    14281 atattttgcc gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg
    14341 aataagggac tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt
    14401 atttcctaaa aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat
    14461 acattttatg gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca
    14521 ttatgcaagt atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct
    14581 acttagcctt ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata
    14641 atacgcaaat tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta
    14701 attttttctt agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa
    14761 aaaaaggtac tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat
    14821 gctcattgga attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc
    14881 aattaaatgg aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg
    14941 ctagtttgtt agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg
    15001 tcttcactat gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa
    15061 agaatcaagt aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg
    15121 ttagtcaaat gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg
    15181 caaaagtagc tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa
    15241 aaggatttca agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg
    15301 cggatgaatt gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa
    15361 atatcatgat ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg
    15421 aaccagaaat cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag
    15481 ctggtactga aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa
    15541 tcgtacaaga tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca
    15601 atgtgctgat tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg
    15661 aaatgggtgc agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata
    15721 tcttacctac agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc
    15781 aaatgacagc tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg
    15841 aaaatcgttt ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg
    15901 gaaatgtagc aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag
    15961 acttgcgggc agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg
    16021 tgcgtaactt gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat
    16081 taggtgcaga cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa
    16141 cgattgctta aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa
    16201 agattttagt ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat
    16261 atggtgtcat cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata
    16321 ttcgagactt ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag
    16381 ccatgaagca gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc
    16441 tttttttagt aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt
    16501 tagaggatat cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac
    16561 ctgttccaat tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat
    16621 ggggactgca cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc
    16681 attgacattc cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt
    16741 tgtgaaaaat acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta
    16801 acaactccgc aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga
    16861 tttaccatgg tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt
    16921 ttccagaggg agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta
    16981 tgtggcaagg gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg
    17041 aaaggggatt ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc
    17101 aaaccaaact cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta
    17161 tcaaaaaatg ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc
    17221 tgagcccatt tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt
    17281 acctaaaaat agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa
    17341 tagatacatt acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt
    17401 ttttgatttt ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg
    17461 taagggacaa tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt
    17521 ttataaaaaa atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt
    17581 agtgaataat gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt
    17641 taatattttc cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc
    17701 caactgtgcc ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc
    17761 ttttgttgtg gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa
    17821 aggggcacat ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt
    17881 cagtaaactt ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga
    17941 taaaaatgct tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc
    18001 aaaagaagaa gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt
    18061 ttcgtttgat ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat
    18121 tgatgagatc aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga
    18181 accgcagatt ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa
    18241 ctatctcgct gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa
    18301 aagaagagaa tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta
    18361 tgataaaata tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt
    18421 atagaagaag tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata
    18481 gggaaaaaaa tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt
    18541 ggtgaagaag attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga
    18601 cactggaatc tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa
    18661 tctggaaaat taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg
    18721 aagtggctaa acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac
    18781 tctcctccat ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat
    18841 gcgttggttc gaatccagct accccagtat ac
//
LOCUS       DAZKOB010000051        18753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000051 DAZKOB010000000
VERSION     DAZKOB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.051"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..200
                     /locus_tag="ACG8M6_001685"
     CDS             <1..200
                     /locus_tag="ACG8M6_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0457353.1"
                     /translation="RGLNEHSNGLLRKDGLPKEMEFNQVNQGFISSVASKRNHIPRKS
                     LNYQTPLEVFLSYVNGKFCLA"
     gene            complement(197..778)
                     /locus_tag="ACG8M6_001686"
     CDS             complement(197..778)
                     /locus_tag="ACG8M6_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0457354.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFIDYYLSN"
     gene            complement(965..1615)
                     /locus_tag="ACG8M6_001687"
     CDS             complement(965..1615)
                     /locus_tag="ACG8M6_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457355.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(2158..3093)
                     /gene="whiA"
                     /locus_tag="ACG8M6_001688"
     CDS             complement(2158..3093)
                     /gene="whiA"
                     /locus_tag="ACG8M6_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0457356.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(3127..4125)
                     /gene="yvcK"
                     /locus_tag="ACG8M6_001689"
     CDS             complement(3127..4125)
                     /gene="yvcK"
                     /locus_tag="ACG8M6_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0457357.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(4122..5006)
                     /gene="rapZ"
                     /locus_tag="ACG8M6_001690"
     CDS             complement(4122..5006)
                     /gene="rapZ"
                     /locus_tag="ACG8M6_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0457358.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(5141..5872)
                     /locus_tag="ACG8M6_001691"
     CDS             complement(5141..5872)
                     /locus_tag="ACG8M6_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0457359.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5876..7141)
                     /locus_tag="ACG8M6_001692"
     CDS             complement(5876..7141)
                     /locus_tag="ACG8M6_001692"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0457360.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7404..10220)
                     /gene="uvrA"
                     /locus_tag="ACG8M6_001693"
     CDS             complement(7404..10220)
                     /gene="uvrA"
                     /locus_tag="ACG8M6_001693"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0457361.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10230..12224)
                     /gene="uvrB"
                     /locus_tag="ACG8M6_001694"
     CDS             complement(10230..12224)
                     /gene="uvrB"
                     /locus_tag="ACG8M6_001694"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0457362.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12483..13985
                     /locus_tag="ACG8M6_001695"
     CDS             12483..13985
                     /locus_tag="ACG8M6_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0457363.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13987..14724
                     /locus_tag="ACG8M6_001696"
     CDS             13987..14724
                     /locus_tag="ACG8M6_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457364.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14895..16088)
                     /locus_tag="ACG8M6_001697"
     CDS             complement(14895..16088)
                     /locus_tag="ACG8M6_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0457365.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(16167..16373)
                     /locus_tag="ACG8M6_001698"
     CDS             complement(16167..16373)
                     /locus_tag="ACG8M6_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0457366.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16366..17508)
                     /locus_tag="ACG8M6_001699"
     CDS             complement(16366..17508)
                     /locus_tag="ACG8M6_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0457367.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            18020..18655
                     /locus_tag="ACG8M6_001700"
     CDS             18020..18655
                     /locus_tag="ACG8M6_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0457368.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
BASE COUNT         5430 a         3976 c         3261 g         6086 t
ORIGIN      
        1 aacggggatt aaatgaacat tcaaacggac tcctccgaaa agatggacta ccaaaagaaa
       61 tggaattcaa ccaagtcaat caaggattca tctcatccgt tgcgtccaaa agaaaccata
      121 tccctagaaa atcactaaat taccaaacac cattagaagt ttttttgagt tacgtaaatg
      181 gaaagttttg tctcgcttaa tttgacaaat aataatctat aaacatcacc ccaaataaaa
      241 cagaaaacag tataagcatt ataaaaatta tcaacaacct tgatccttct tttatagatt
      301 caggaaacag tataatttta gtactagata aattgtttac accgattagt gttaaaatat
      361 ctttattctt cttgctaaca taacccttta tgcacaaaag aacagctata cttaaagtaa
      421 caaataaata acgtattcca atagaaaaat cactataaat actctctagc ataggaccga
      481 caattgaaga agtcactact gcaaatgctg gacctatcca agcaacatta gaatgttctt
      541 tctctctttt cctttgtaaa tctttgttga gtattaatct atttatttct tctgcaggca
      601 agttaatttt cataacctta tgggggatta tccaattaaa aatcggaaaa aggtagcctc
      661 cccaaaaagt atagtgatct gtgtcaatca gataataacc tgattttgat tccagaataa
      721 catatctcaa agccgaacga aaatttttca tgaatatcca taatctcact gtttccacac
      781 ctaattcctc caattccaat taaatggatt tatagcatca ccaattatgt taactgcctg
      841 accaacctca ttgataaaat tggcaccact atcccatgct tcaacaatgc tatatatcta
      901 tcaaacaaaa aatggaaaac taaatactcg cgcacataaa ttttacattc tgttttttat
      961 aagactaata ataatcattg aaaaaatttc ttatcggtgg cgcatattcc caattgatta
     1021 atgtgatccc atattctgca agtattttac gagcttcttc taagtcatag tgagaaaaat
     1081 gcataaaacc tgcaatacga tcacggactg tgagctgtgg cttgcttcca ccatcattca
     1141 taaaatatcc agcagagcca ctacttaaag tgattgttac atcttcactt tctattatta
     1201 agaattcata atgaaatgct ttcaactctc cactattatt taaaagtttt atactccaac
     1261 ttcgtctagg ttgagttgtc acattccctc taaaacttcc tataaattct gcaatcaccg
     1321 tatcttcaat caaaattctt tcctcatcta tttcatattc aagactaaac ggaaattctc
     1381 catatgtaat ctctggcaat ggtggtgctg tagttctcca aataaataca ataactgtga
     1441 atagaaaaaa tccaacgatc gatataaatg ttatgagacc tgctcgaagt aaaacccgat
     1501 ataataaaaa tgctgtattt cgctttgcta tatctagaaa aataaaaagt actgcaatca
     1561 taatgatagt aaaagttgta aaaaagcaca ttttttgccc tccctttata aacatcgatt
     1621 ctcaaattct aaaccaaatc ttgatagttc aagttcaggt ataacgtata ccaaccatac
     1681 atcccaatta ctatttatga acttatgatt atcactgaat aaatcactcc atccccttgt
     1741 taaaacttct acattaatta cacctggttg ttttcgacta gctaatccaa tgaacttatc
     1801 aaccatcctt atccaccaac cacgagaatc ttgaccagaa gtaagaaaaa cagttctcaa
     1861 ggttgatcga gcagttcctt ccagttttta attaatctcc agttatagat aaatacttga
     1921 tcctatctat atcaggactt ctatattaag caaaagttta tgtataaaac taaaatcttc
     1981 attcagttgt ctccccgctt tttatacgat tgtattaaaa aacaatagaa gcctttcaac
     2041 attatgttac tccagtaaag ttggaattat agtttgacgg aagaaaagca agtatagctt
     2101 ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt ctttggatta
     2161 aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc ggtgattaat
     2221 tcccgatttt gatattgcac ctgagggaat catttcccct aattctttca agctgacctc
     2281 tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg catgtagacc
     2341 gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat caatcgtctt
     2401 gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca tatcccgtac
     2461 gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg ttaagaaatc
     2521 agcaattttt tctgctcctt ttaaatacga aatatagcca ttgcgccgtt caagcgttcg
     2581 agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct gttcataaat
     2641 agaaaatatt tccaaatgat aacgactcgt ttccggatta ttgactgaac ctgaggccat
     2701 aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct cgtccgaaac
     2761 gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat cttttgtgcc
     2821 ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt tacgaacgag
     2881 tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc tggcaattgc
     2941 agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta aagagccgtt
     3001 cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt ccaaaccagt
     3061 tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc tccaacaata
     3121 aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta ctttgtctcc
     3181 atcgtggaag acaccaccat ctttcaattc taaaaagtcg gtggaaatca ctcgacatcc
     3241 ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat catagatctc
     3301 tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat cgataaaagg
     3361 ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat gttccgtttc
     3421 ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct tgagcattgc
     3481 ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac ttccgggtcc
     3541 caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg ctttggcttc
     3601 acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt tgatcgcgat
     3661 tttagattct cctactgcag tcgttccgtc tttaaaaaca gcatgcaagg tcaaaggagt
     3721 ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata attgaatcgc
     3781 ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac cgatcgtatg
     3841 attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt catagaattg
     3901 tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag ccatagcgat
     3961 cgattctctg attgcaccgc tgcttccacc atcatctgca acagtcacta ctgctgtgat
     4021 atctgctcct tgatttcgta aacttttcag gataactggc agaccagtgc caccgccgac
     4081 cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg gttcaccgtc
     4141 tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt ccctaaagca
     4201 tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca gccgattgca
     4261 actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc cagaagatcc
     4321 aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc atagactggc
     4381 tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg caaaaaacgc
     4441 acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa tgatacaaat
     4501 tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt ttcacgtaat
     4561 tgtcgtggtg tcaatgttgt cgtatcaatg atcacggaag cttgggcttt caagtcgtct
     4621 agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc cattggatgt
     4681 gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc taaaaataag
     4741 atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg gatttcttca
     4801 aagaaggaac gagaacgcaa atccactcct aaagccattt ttgtgacttt acctgattct
     4861 ttgatcaatt cccagaattt tggaatcaat cgaggcggca tattatcgat acaaaaatag
     4921 cccatatctt caaaactttg gatagctact gttttccctg caccactcat acctgttatg
     4981 atgactaatt gcaaattatc tgtcatattt acgaacccct ctcacattgt gcataataaa
     5041 attatatcat cccgggcgta tcattaccag ttttcttttt cttttacaaa aagatttgga
     5101 ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc gatgctgtat
     5161 ccaatgcctc gcttctttca gctaatgcac gttcgatcgt tcgatccgct aaaatagatt
     5221 gagcatcgat cactgggtaa tgattatctt cagcaaattc atgcatcaaa gataattctg
     5281 tgcaacctag gatgactttt tcacagttta ggcgctctac cgcttcctct aagatttcgt
     5341 aatagagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc aggtaattga
     5401 ttttttcttg aagcgcagta tcaggaatca ctgtttcgaa accaagattt ttgacttcgc
     5461 gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact ctccctgtcg
     5521 tatgctgacg gacaagttca tttgctgctt ctcttggcat atgcaagata ggaatatccg
     5581 ttgccgcttg taactcttca aaaaagtagt gtgcggtgtt acaagtcaac acaatgaagt
     5641 ttggtcgcaa taaattttgt ttctcaatat catccagcag aaacggcatt ggattttctt
     5701 ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg aataagacat
     5761 aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag cgaacaaaac
     5821 tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc atgaactagc
     5881 cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac atatatttca
     5941 tcaacaacca gcgcttgatt gatcggtctt tttcataaaa tacagtagta ccgtaacgat
     6001 tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca tattctaaaa
     6061 agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc gttccatagg
     6121 tcgtttcaac aaagggtttg ttgaacactc gatcttctgt gacaaaacga gccagattca
     6181 agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt tcaaataatt
     6241 tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct gtatattcga
     6301 ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct ggcatgatca
     6361 caacgtaatt accaatcgat gctggtgtag gatcttctaa tagtggatga ccaaggcaca
     6421 tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata ttactatcat
     6481 cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg tatatccggc
     6541 ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt cttccttcaa
     6601 attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg aattcaaaag
     6661 gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc aatgttttag
     6721 gatacggaag atcatattct tcacaaactt cataaaagct tactttattg atcagccttt
     6781 caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct tgtttatgtt
     6841 gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt ttactcttat
     6901 cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaatacaggg tctttatcaa
     6961 atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct aactgatccg
     7021 aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc ataccataaa
     7081 cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata ctgttcatca
     7141 ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa ggaatatcat
     7201 accataaaga aaaaagcaat caaaggaaag tcctccgatt gctcaattct tttttgaacg
     7261 gattgaactt ataggaagtt tttatctata ccaatcagtg tagaaagaac taccatgtaa
     7321 tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta tgttttttta
     7381 actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg acctgtataa
     7441 ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt tcctccacct
     7501 tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac atctagattg
     7561 tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac atgcaacaag
     7621 cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata gaaattcttt
     7681 ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc accaccggac
     7741 aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac aatcgtttgc
     7801 agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcctc tactgtcatt
     7861 tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc agaattatat
     7921 cgtttaccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg catttcgatc
     7981 ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt gaaactaaaa
     8041 cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa cccacggata
     8101 tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc gattgggctt
     8161 tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt gaatttacct
     8221 ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat actattgacc
     8281 aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc taatggaaat
     8341 tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat tgctttccca
     8401 ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag atatttccct
     8461 gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac gatttgtccg
     8521 ccttgttctc cggccccagg tccgacgtca atcaaataat ctgctgccca catcgtatct
     8581 tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt taatgagcca
     8641 atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa aatatacaaa
     8701 actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc ttctccccca
     8761 gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt ttccaagaat
     8821 gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc ttgttcagat
     8881 aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt tgtttgcccg
     8941 atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata acctttacat
     9001 gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa atcactgttc
     9061 gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt ttctacatcg
     9121 cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt cccattcaag
     9181 atgatctcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc gataccgaaa
     9241 ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg attccatgga
     9301 acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc ttgatcgact
     9361 tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc attgaatgag
     9421 aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc atagtgttcg
     9481 ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc tgctaatcgc
     9541 agggccgcct ctaaagaatc aaataggcga gaacgaatcc cttctttcac aatgatccgg
     9601 tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac ttcacttaaa
     9661 tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat tcgttcgaat
     9721 atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg gatctttgtg
     9781 cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact ggtaatctcg
     9841 atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg taaatagtca
     9901 ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt tttctgatca
     9961 atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc catttgtcct
    10021 aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc ttcagcgtac
    10081 agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac gaccacgaat
    10141 ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc accatgaatc
    10201 gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc taaaatcata
    10261 tctcgcacat tcgcggctgt ttcaaaatcc aaagcttttg ccgcatcttt catttctttt
    10321 tctaatttca tcaataacgt gtctttttct tccttcgtca tttcttcgta cgagacttgt
    10381 accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc tcggatttct
    10441 ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg aatcgtacga
    10501 cgtcttgacg tttcatccat cgctagacgc atagaatctg ttactttgtc tgcgtacatg
    10561 attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa ggaacgttca
    10621 ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac ttcaggtacg
    10681 tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc taatcgaaga
    10741 tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata tttgactttg
    10801 atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt caatgtcgtt
    10861 acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag atcatcgatt
    10921 tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg acggatgatt
    10981 tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc agagacataa
    11041 atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt gtctaaagca
    11101 gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc accattatac
    11161 attcctctga tttgaggcat ggttacgtgc gattcgtccg ccacgatcaa aaaatcttca
    11221 ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc catatgccga
    11281 gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat atcgtaattg
    11341 gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa gacagttaaa
    11401 cgctgttcta attcctcttt gatattcgct acggcatgct ccatatgttc atcattcgtc
    11461 acaaagtgag tcgccgggaa gattgctacg tgttccgttt ctcctaaaac ttcacctgtc
    11521 agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac acgtaaggca
    11581 cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg aaaacgaccg
    11641 cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg aatcaactga
    11701 ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta ctcaaaaggc
    11761 gagcctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt tctttctaat
    11821 aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact tgaatctttt
    11881 tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc ataataagaa
    11941 acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta cagctgacca
    12001 gccaatgttt tgttatgggc aataatcagt gttggtttat tcactttctc aataagattg
    12061 gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc ttttttcccg
    12121 ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc cggttggtat
    12181 ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg tcctcctttt
    12241 cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag gcattcctta
    12301 ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat tatacatgtt
    12361 atgttttata acataaaatg ttagaaactc gcattttttt cattttttcg agcttacagt
    12421 cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt aggaggttga
    12481 ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga cttgtgacac
    12541 ttgcgtttgg tcatgttgca caagctgaca acactacaac ggcagagtca agcacaagcg
    12601 agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt gactctgctt
    12661 ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat gtaaaattga
    12721 tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt ggtttctctg
    12781 ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga atgactgtaa
    12841 cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc gggatccaat
    12901 tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa ggaaaaacag
    12961 tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa tctgaatatg
    13021 gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta aaagttggcg
    13081 caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa caaggacaag
    13141 aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc gttaaaaaag
    13201 gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta aaaagaacag
    13261 gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact tccagtgagt
    13321 cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat caagcattgt
    13381 tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg gctatagtaa
    13441 tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc acaattgcta
    13501 gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc tttattttct
    13561 ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt gttatcacct
    13621 tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc aacgccgttc
    13681 ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg acaatgcaaa
    13741 aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat cagttcgtta
    13801 tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg ctgcaaactg
    13861 gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc attgcgatca
    13921 tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa aagaaggtga
    13981 agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg cgataacaca
    14041 gtattaaacg acatcaatgt atccatcaac gaaggagatg tcgtctgtat cattggccct
    14101 tcaggttcag gaaaaagtac attcttgcgt tgcttgaatc gtttagaaga accttctgac
    14161 ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat caacttggtg
    14221 agacaacata tcggaatggt attccaacac tttaacctat tccctcatct ttctattttg
    14281 gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga agcagagaaa
    14341 aaagcaatca aattactgca gacagttggt ctagaagata aaaaagatat gtatcctgaa
    14401 atgctttctg gtggacaaaa acagcgtgtg gctattgctc gtgctctagc gatgaaccct
    14461 gacatcatgc tttttgatga gcctacttct gcactcgatc ctgaaatggt cggtgatgtc
    14521 ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt tacgcacgaa
    14581 atggggttcg ctaaagaagt ggccaatcgc gtcatgttca ttgacgacgg aaacttttta
    14641 gaagatggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac aaaagatttc
    14701 ttgaataaag tattaaatat ttaaaataaa ttatatatgt ctttttgact atctttttat
    14761 atcttcttaa atgtccgcaa agccttattt tataggctct acgggcattt tgcttttgtg
    14821 gtaaacctca catatctagg tctatcttct tatattttca ctatcaagcg tggttaaaat
    14881 cgtggtaaat acttctatgc aattagacgc tgaacctctg attttgcggt atctatggac
    14941 gcatgagcat accagttcat tgtgatactg atatttgaat gccccatgat atactgtaaa
    15001 tcttttgggt tcatgttctt gcttgccagt cttgtgcaga atgtatggcg tagcgtatgc
    15061 ggtgtgatat gtggcaatgg attgtccttg tggtgcttgt tgtatttctt taccatacgg
    15121 ataaataagg cgttataatc aatcgcaact ttgggcttgc ccttatgatt gacaaatagg
    15181 aagttgcccc gtccgtctat cacaaatggt tctgcctttg gacgtttttt cataacctgt
    15241 tgaaatgcct gtatcgtttc tttgcttaat ggtacttgcc ttattccgct ttttgtttta
    15301 ggcgtttcaa tataatagcc ctgttccttg ctctttagta actggtggtc gataatcaca
    15361 acttcattct tgaaatcaat gtcggctact gtcagtccgc acagttccga aatacgaagt
    15421 cctgtcttta acagtatcag cacatcatca taatgcttgt gatacacatt gtccgtcttg
    15481 atgaatgaga gtaaggcttg ttcctgttcc tctgtcaatg cgactttctt tttggtatca
    15541 ttttctagga cttcactcaa cttgaaatca aagggatttt tcctcacata atcatcttga
    15601 atggctatgt agaatgacgc ttttaacgag cgtttatggt tgttaatggt attgtaggaa
    15661 aagcctttgt ctttcatgcg taacgcccat tccttagcgt cagagggttt gattgtatca
    15721 atgctcctag cacctaactt gtcctctttc aataaccgca tgagttgttc ccgttgtttc
    15781 tgtgtgcttt tcttcacatt tgccctctgt gcgttctgtt tggcataaag ttggcaaagc
    15841 gtcattttct tgccagtgct gtcgataccg tcctcaatat cccgtcttat ctgctgttcc
    15901 agttctcgaa gtgagggttt ttcccgtttt ccctttggtg tcgggtctgt gggtgtcaat
    15961 ctccaagcat acacatattt tgtgtttcca aatgcgtcta catatttata taagtatttt
    16021 ctgtctgttc gttggctctc tccagtatgc aggatacgac ctttgttatc ccgtcttttt
    16081 tctttcatgg tgtctgctcc tttccttgtt ggaaagagcc ttgatatgac ttgtgttcat
    16141 cataacacat acaaggctca tttgcattag atagcgtcca atttatcaat gacctgttca
    16201 aactgtcggc gtttaatctg tatgcggttg ccattcataa tgagccagcc agcgtccttg
    16261 ttttcctctg ccaatcgtct taacttgttt tctccgatac ggaaatactt tgacgcttct
    16321 tcaatggtaa gggtgtattt ttcccatacg ggaatatcct gtgcattact cataaaacac
    16381 cccctcactt tcgattacgt ctgctatgtc cgtacacatc tgttccacaa gcatttcctg
    16441 cttttcagag aggtgggtac attcatcaat cattttcatg tagtttgagc ctgtcagcct
    16501 gtcgatttcc tctatcatca cgattgacgg catgacctgt ctttccagcc attgcagagt
    16561 gcgttccata ttgacaggta cggggttcat ttcaaaaggt aaactgtcat gggaaatgaa
    16621 tatctcccac agcggatagt cgggaaattg cacaaaatca gtaataaggt aaaacaccag
    16681 cccatgaggg ttatatgtca ataacaattc ctcaactgcc tgtactgctt ttgtaccacg
    16741 cagacgaatt tcaaaacgat tgataatatc cgtatgtttc tttttggttg cctgttcctt
    16801 ttgtttttca tacaggcaga agtattttgt gcttgccttt gaaccgatat aaagggtgct
    16861 tgctaaatta cggttttttc cgcttagttt tccaccctgc acattttcat aatttttaca
    16921 gcgacaatcc gcaccgccat ttctgtattt ttcagacaaa acgggaatat ccaacagacc
    16981 acacatatca ttgattgcca agtcaaaacg tcggataaca ccgtcacagt caagacagcg
    17041 gtttaaaaat gaataccaat ctctgttctg tgcctgcaag acatattcca tgttgcgtga
    17101 gcctgcccct tttaattcca agaatacgcc catgtgttcg tcatcagaag ccatgacctt
    17161 gatttctccg taggcgtaat gctctttata gccataatac ccatagtcat aatggataaa
    17221 ataatctgct ttcatgccaa gcacctttcg gataatttcc aatgcgtcag tcgttggata
    17281 acggacagac agataatcaa cgagcagata aaaaggctta tcgcagatat aataattcag
    17341 acaacgcagg ataatatttt gtgtttcctt gctggcgttg gcttttccgt tctcaaagcg
    17401 gttgatacta tatcttgata catgggtaag acttgcaagt tctccttgtg aaagaccgct
    17461 tgcaagacgt ttgttcttca tttcaagtgc aaattctaaa ttgttcattg tattcctcca
    17521 atcccacaat agccttgaaa tacctgtaaa cctctattca aaaagtgtga tatgcactat
    17581 cttcttaaaa cttctcatta ctactcacaa ctttgggatt gtatggtttt tgacttgtct
    17641 tttgtgaaat agtgcccccc tgttagataa cgggggcaca gacaaggctc gcaggctcgc
    17701 cagccgacaa agcatttctt ccccttgaca caaggtgtca tggcggtaag cctgagaggc
    17761 ttccgccacc gtgtcagaaa tactttgtca gcaagttctg ttttgccttt tctaacaagt
    17821 tccatttcaa ccgatattca tatctacgga tttaatcaga taggtatttc tttttgaatg
    17881 tttttatatt tcattctatt gtgcttattc gattacaaga acatagtaat tcttatatgc
    17941 ttatttgtca atatcttttt ttgcctttac aaatcatatt aagcatgata gacttataat
    18001 agcgaagata taacgagata tggagggtta tgcaatgaaa ataagccatt taggaaataa
    18061 tatacagacc ataagaaaat ttaggggaat gaaacaacag gaacttgcgg acaaaatcgg
    18121 tatcaatatg cagagccttt ccaagattga aagagggttg aactatcctg cctatgaaac
    18181 gctggaaaag ataatggaag tgctggacgt tacgcccaat gaattgttgt cgggagaatg
    18241 gaagtatgtc aatcaatctg aaaaggaagt ctgtcagttt ttaagagccg aagaacgtct
    18301 aaatgcagaa ctgaaacatg gacactatga taacttcttt gacagcgagg aagaatggct
    18361 ggaatatgag ttagaaaagt tacgggaata tattacagac tatatcaacg ggaaaagcat
    18421 tgaagcgtcc gacctttacc caatcaaaga atttatccag catttgaagt ttcaaaaatt
    18481 attagaccgc tatgatgatt tatacagtat ggatatgttt ggggaaagca tagagggaca
    18541 taagtataag acaccttatc aagtcgtgaa aatgataaac ccgaactcaa aagaagatat
    18601 ggaactgtta cgagaagtat taaggaataa caattttgat gatgaggacg agtaactttt
    18661 ttcctcttgc gagaagatac agcaaacaaa aagcccgatc aagagtgcaa agcaccacca
    18721 atggtggcaa agctcttgac agggcttttt ctt
//
LOCUS       DAZKOB010000052        18528 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000052 DAZKOB010000000
VERSION     DAZKOB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.052"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..770
                     /locus_tag="ACG8M6_001701"
     CDS             123..770
                     /locus_tag="ACG8M6_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0457369.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    899..1115
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1163..3808
                     /locus_tag="ACG8M6_001702"
     CDS             1163..3808
                     /locus_tag="ACG8M6_001702"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0457370.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4118..5431
                     /locus_tag="ACG8M6_001703"
     CDS             4118..5431
                     /locus_tag="ACG8M6_001703"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0457371.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5421..6074
                     /locus_tag="ACG8M6_001704"
     CDS             5421..6074
                     /locus_tag="ACG8M6_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0457372.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6129..6821
                     /gene="radC"
                     /locus_tag="ACG8M6_001705"
     CDS             6129..6821
                     /gene="radC"
                     /locus_tag="ACG8M6_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0457373.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6971..7171
                     /locus_tag="ACG8M6_001706"
     CDS             6971..7171
                     /locus_tag="ACG8M6_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0457374.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7425..9104)
                     /locus_tag="ACG8M6_001707"
     CDS             complement(7425..9104)
                     /locus_tag="ACG8M6_001707"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0457375.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9106..9318)
                     /locus_tag="ACG8M6_001708"
     CDS             complement(9106..9318)
                     /locus_tag="ACG8M6_001708"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0457376.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9711..11441
                     /gene="efrA"
                     /locus_tag="ACG8M6_001709"
     CDS             9711..11441
                     /gene="efrA"
                     /locus_tag="ACG8M6_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0457377.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11438..13198
                     /gene="efrB"
                     /locus_tag="ACG8M6_001710"
     CDS             11438..13198
                     /gene="efrB"
                     /locus_tag="ACG8M6_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0457378.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13293..13490
                     /locus_tag="ACG8M6_001711"
     CDS             13293..13490
                     /locus_tag="ACG8M6_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457379.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13643..14128
                     /locus_tag="ACG8M6_001712"
     CDS             13643..14128
                     /locus_tag="ACG8M6_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0457380.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14232..14825)
                     /gene="clpP"
                     /locus_tag="ACG8M6_001713"
     CDS             complement(14232..14825)
                     /gene="clpP"
                     /locus_tag="ACG8M6_001713"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0457381.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15005..15532
                     /locus_tag="ACG8M6_001714"
     CDS             15005..15532
                     /locus_tag="ACG8M6_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457382.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15623..16360
                     /locus_tag="ACG8M6_001715"
     CDS             15623..16360
                     /locus_tag="ACG8M6_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0457383.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16364..17281
                     /gene="miaA"
                     /locus_tag="ACG8M6_001716"
     CDS             16364..17281
                     /gene="miaA"
                     /locus_tag="ACG8M6_001716"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0457384.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17283..18512
                     /gene="hflX"
                     /locus_tag="ACG8M6_001717"
     CDS             17283..18512
                     /gene="hflX"
                     /locus_tag="ACG8M6_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0457385.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5990 a         3204 c         4026 g         5308 t
ORIGIN      
        1 atgcaagctt ttgaacaccg ttttatttgt ctcacaccct tttccttcct acttgtaacc
       61 ttgctcccgt gttagaaaca tgttacacta aatatgtgaa attattaaca aggagcgtcg
      121 ttatgcgaga aaaaacagat acactgccaa aagcaactgc gaaaagatta cctttatatt
      181 tacgctactt gaagatgctg gatgactcag gtataagtag aatcaaatca aatgagttca
      241 gcgaaatcac ccaaattcct tctgcaacga tccgcagaga cttctcgcaa ttaggcgagc
      301 taggcagaag cggatatggc tatgatgtcc cttttcttat cgatgtattc aataatatcc
      361 ttaatactaa agaagaaaaa cgaatcgcat tagtaggata tggaaatctt ggcaaagcat
      421 tgaaacacaa caattttaga agaaatgaaa atctaaatat cgtgtgcgtc tttgataatg
      481 atccagcact gatcaatcga gtaattgatg gagaaatgat ctatccgatt gatcgtttcg
      541 cggagatagc caaagcaaaa aatgtgacag tagcaatctc tacggtacca agtaaatatt
      601 cacaatctgc tattgatgaa attgtcaaag gaaatgtaac cgctatactt aattttgcgc
      661 ctgacagagt aactgtccca gcttatgtca atgttcaata cattgatttg acaaccgagt
      721 tgcagacgct tatttacttt gatgagaatt actcagaagt tttttcttag gaaaatttga
      781 gtaggcttat tgatcttgcg agaactcgtc gtaattcttt caaaaatttt ttagtactaa
      841 atgaagtggt tttttcacga ccacttgttg actaaaaaaa aaaaccattg tagaatgaag
      901 cataacgctg aacaagagga gtaactggca cattcattct tagagagaga actatctgct
      961 gaaaggttct caatgacaac cagtgaaggt agcttgggag tttctgtctt gaatggagta
     1021 gagatagacg agtcgtgatc gttaactcac ttgagaggca ataatgattg attattgcaa
     1081 tttaggtggt accgcgtaaa tcatacgtcc tagaatacga aagtatttta ggacgttttt
     1141 tagtttaaag gaggaaaaca gcatgacgga agaaaataac ctatcaacaa aatataatcc
     1201 gcaagaagtt gaagctggac gttatcagca ttggttagaa caagatttgt ttaaaccgag
     1261 cggagataaa aaagcaaaac cttattcaat cgtaatcccg cccccaaatg ttactggaaa
     1321 acttcattta gggcatgctt gggatacgac cttgcaagat atgctgattc gtcaaaagag
     1381 aatgcaagga tatgatactt tatggctgcc tggtatggac catgcaggaa tcgcgacaca
     1441 agcaaaagta gaagcgaagc ttagagagca agggatttct cgttatgatc taggtcgtga
     1501 aaagttcatc gatcaagtgt gggaatggaa agaagagtat gcgagccaca tccgcgaaca
     1561 gtgggctaaa atgggcttat ctttagatta cagtagggaa cgttttacat tagatgatgg
     1621 attatctgaa gcagtacgta aagtttttgt taccttatac gaaaaaggtt tgatttatcg
     1681 tggtgaatat atcatcaact gggatcctca agcacgaaca gctctatctg atatcgaagt
     1741 tattcataaa gatatcgaag gcgcctttta tcatatgagc tatgaactag cagatggatc
     1801 aggcgtagta gaaattgcga caacccgtcc agaaacgatg ttgggagata cggcgattgc
     1861 tgttcatcca gaagacgagc gttatcaagc gctcatcggc aaaaaagtga ttctaccttt
     1921 agtcaataaa caaatcccaa tcattgcaga tgaatatgta gatatggaat tcgggacagg
     1981 tgtagtaaaa atcactccag cacatgatcc aaacgacttt gaggtaggta atcgtcatga
     2041 tcttccacgc gtaaatgtta tggatgatga tggtacgatg aatgaactag caggaaaata
     2101 tgctggaatg gaacgttttg ctgctcgtaa agcaatcgtg caagatctga aagaaatcgg
     2161 tcgattgatt aaaatcgata cgatgatcca cagtgtgggt cattccgagc ggacaggtgt
     2221 cgttgttgaa cctcggctgt ctactcaatg gtttgtaaaa atggcgccgt tagcgaaaaa
     2281 agccattgat aaccaagaaa cagatcaagc agtggaattc ttccctccaa gattcaatca
     2341 aacgttttta cgttggatgg aaaatgtcca tgattgggta atctcacgac aattatggtg
     2401 gggacatcaa attcctgcat ggtatcacaa agaaacaggt gaaatgtatg tagggatgga
     2461 agcaccagcg gatagtgaaa actgggtaca agatcctgat gttttagaca cttggtttag
     2521 ttcagctttg tggcctttct caacaatggg ctggccagat acggaaagcg aagattataa
     2581 gcgatacttc cctactagca ctttagtaac cggttatgac atcattttct tctgggtaag
     2641 cagaatgatt ttccaaagct tggaatttac tggcgaacgt ccatttgaga atgtcttgat
     2701 tcatggattg attcgtgacg aacaagggcg gaagatgagt aaatcattag gtaatggaat
     2761 cgatccgatg gaagtaatcg aaaagtatgg agcagatgct ctccgctggt tcttatcgaa
     2821 tggttctgca ccaggacaag atgtccgctt cagctatgaa aaaatggatg cttcttggaa
     2881 ctttatcaac aaaatctgga atgcttcacg tttcgttatc atgaatatcg aaggaatgac
     2941 cgtagaagag atcgatttat ctggagaaaa atcagtggct gaccggtgga tccttactcg
     3001 tttgaatgaa acgatcgaac gagtaactga gctatttgat cgttttgaat ttggtgaagc
     3061 aggtcgacaa ctgtataact ttatctggga tgatttttgt gactggtaca tcgaaatgag
     3121 taaagaagta ctttatggag atgatgctgt ttcaaaacaa accacccgta gcatcttagt
     3181 ccatacactt gatcagatcc tacgtttatt gcatccaatc atgccattcg ttacagaaga
     3241 aatatgggaa catattcctc atcagggaaa ttctctagta gtggcagaat atcctgttgt
     3301 acgtccagag tttgatgatg aaacagcttc taaagggatg gaagtattga aagaattgat
     3361 tcgagccgtt cgaaatattc gttcagaagt gaatacacca ttgtctaaac caattacgtt
     3421 gctaattaaa acaaacgatg ctaaaatcga tcaattttta gttgaaaata caagctatat
     3481 cgaacgcttc tgtaatcccg aagagttgac gatatcaagt gagatcactg cgccagatct
     3541 agcgatgtca gctgttctga caggtgcaga aatcttcttg ccgttagcag gattgatcaa
     3601 tattgaagaa gaaatcaaac gattagaaaa agaactggaa aagtggacaa gcgaagtaaa
     3661 acgtgtccaa ggaaaacttt ctaatgaacg ttttgtttct aacgcgccgg aagaagtagt
     3721 agaggcagag cgtgcaaaag aaaaagatta tttagagaag caaacggctg tcaaagaacg
     3781 aatcgaaagt ttgcgtacta ttcaataaga atagaaaaag agagtgtgaa atcagctagc
     3841 tattactaaa agctagctga ttgaacatcg ttacaggaaa gagtttgagc ctcactctta
     3901 gcttcgattc ataaggagat gtgacataaa ttttgtcgca tctctttttt agtctactct
     3961 tattatgtag gagaaggagg gaggtggatc atgtatcaag agtcaataaa gtagttgata
     4021 aaatcgttcc tctctctgtt tcttttctct ttgtcatact tttatttttc ttatgtgttc
     4081 tatttgttat aataaaagag aaatggggga attgttgatg acgattgaag aagcaataga
     4141 atggatccat agtcgattac catttggttc acgtcctggc cttgaccgag tcgaagcttt
     4201 attagaagtg gtagggcatc cagagagaaa agtaaaaacg atccatatcg ctggaacaaa
     4261 cggaaaaggt tctactgtag cttatttgag aagcctatta gaagaggctg ggctgacagt
     4321 aggtacattt acttcacctt atatcgaaac gttcaatgaa cggatcgcaa tcaatggcaa
     4381 aagcattcca gacaatgatt tgatccagct tgtaaaaaaa tatcagccac tgatcaaaca
     4441 acttgatcaa tttgaagcag tagctggtat tactgaattc gagacattaa cagcaatggc
     4501 atttgattat tttttgatga agaaagtgga cgtagcgatt atcgaagttg gcttaggcgg
     4561 cttattggat tctaccaatg tagctgtacc aatgctgacg ggaataacaa cgattggact
     4621 tgatcatacc gaaatacttg gagatacaat tgaagaaatc gctgcacaaa aagctggaat
     4681 catcaaacaa ggcgttccag tagtaacagg aaatattgcc ccagaagctt ttactgtcat
     4741 ccaacaaaca gctaaagcaa aaaatgcacc gctttatgta tacggggaaa attataaaat
     4801 aaactatctg catcctgatg agatgtgggg agaagtattt gaatttttta atgaaatagg
     4861 aaagatccct caattaacca ctccgctttt agggcaacat caagctgaaa atgcaggagt
     4921 agccatcgaa ttgtattttg tttactgtca attggaaaag ctgcctttcc gagaaaaaga
     4981 tgttttgaag ggattgaaga aatctcgatg gccaggaaga atggagcgtt tgagtaacga
     5041 gccattagtt gttttagatg gtgcccataa tacgcatgca atgaaccggc ttgttgagaa
     5101 tttgaaaaaa gaattcaaag gatatcaaat caacattttg ttttccgcct tacgaactaa
     5161 aaatgttaga gaaatgctag aacgattgca gcaagtacca aatgtccata tttatttgac
     5221 gacctttgaa catccaaaag ccatcgaatt aagtgaagta tcagatttag caagtgagag
     5281 agtatcaatc gtttcattgt ggcaattcgg attagcagat atattggaaa agatgacaaa
     5341 tgaagacgta ttagttgtga ccggttcact ttatttcatt tcagaagttc gccatttatt
     5401 aatacaatta ggaggagatc atgaagtata atgcagtgat ttttgacatg gatggtttgc
     5461 tgttcgacac agagttagtg tattacgaag ccacacaagt agtagcagac aagatgggtt
     5521 ttccttatga taaagacctg tatttaaaat tcttaggtgt ttcagatgaa gaagtatggg
     5581 cgaattatca tatgattttc tcggaatttg gcaaaacaag tgtacaaaga ttcatagatg
     5641 attcatacca ggaaacgctt gaacgttttt cgcaaggagc ggtacaaatg aagcccggtg
     5701 tgttggaact cctctctttt ttagaagaac atcagattcc caaagttgtc gcttccagta
     5761 accaacgagc agtcattgaa cttttattga agaagaacaa tctgtttgaa cgatttgaga
     5821 cgattgtttc tgcagaaaat gttcaacgag caaaaccaga cccagaaatt ttcttgttag
     5881 ctaatcagaa gctaggtaca gcgaagaaag aaacattgat tttagaagat tcacaaaatg
     5941 gtattttagc agcttctgcg gctgagatcc cggtagtcat ggtaccagat ctgcttccac
     6001 catcccaaga attgatcaaa aaaacagctg gggttgtttc ttctttacat gaaatccctg
     6061 cattgttaaa ataaaaatcc atctcttttc gtatcttatt ttagataaga tagaaaggag
     6121 atgttttttt gggaaaacga ttgatcaaag aagtacctac tagctcttta ccaagggaac
     6181 gtatggaaat ttatggcgcg gaagctttat cggatcagga gcttttggca atcttactgc
     6241 gtaccggtca acacccttat agtgtgatgt cgattgctgg aaatctgtta aaaacgtttg
     6301 gaggactggc ctctttacga caggctactt tgcatgagtt agaagagatt cgagggatag
     6361 gacgcgtgaa agcaatcgaa atcaaagcct tgatcgaatt aggtcgacga attcagacgg
     6421 atcatgaaag agcggctcca ccggttcgta ccagctacga attggcccag caattgattc
     6481 tggaaatgaa agatcacaaa caagaacatt tggtttgtat ttatttggac acgaagaatc
     6541 aagtgatctt aaaaaaaaca gtgttcatcg gttcgctgaa tcagtcagtc gctcatccta
     6601 gagaaatttt ccattatgct gtacgttatt gtgcagcaag gatcgtttta gcgcataatc
     6661 atccttctgg taatgttatt ccatcgcagc aggatatgaa ttttaccaaa aggatacaaa
     6721 agtgtggaga aatgatgggg atcactgtac tggaccactt gatcatcgga cgcaaacgat
     6781 attttagttt gagagaagaa ggaatgatgg aagagaagta agttgctttc ttgcaaaaaa
     6841 aatgaagcaa gtgtacaatt atattgtaat tttgtttggg aatagaagtt gaaacatctg
     6901 ttgcacctct atcgtacacg agcaatgtta caatttaaat tagtgctttt ggcacgagga
     6961 ggatattatt atggaaaacg gaacagtaaa atggttcaac gctgaaaaag gatttggatt
     7021 tatctcacgt gaagatggaa gcgacgtatt cgttcacttc tcagctatcc aaggtgacgg
     7081 attcaaaaca ttagaagaag gtcaagcagt gactttcgac gttgaagaat cagaccgcgg
     7141 cccacaagct gcaaacgttg taaaagctta attttatctg taaatcaaat tgatggtaga
     7201 catcaatttt tacgagagaa caaagcgagt aacaaaaata cctgccacaa gagacaattt
     7261 ctcttgtggc aggtattttt ttgtatgaaa aagtaaataa atttaagagg ctatgaaaga
     7321 gccaccttgt atcaaggaat aagaacaaac aaaaatggct tctcatgttt ttcatttgtt
     7381 ttctcttata ttcaggacaa ggtgctgatt tcataacctc ttatttattc ttcatctggt
     7441 gtcattacca ttggcaaaat cattggatgt ctttctgtac gttcgaataa gaaaggctga
     7501 agcgcgccag tcatcgcttc tttcaattta tattctgaac agtcaggtcc attcaaacat
     7561 tctcttaaag cgtggaataa gattcgctga gcttcattga tcatttctcc tgattctcgc
     7621 atgtagacaa atccacgaga gagaatatct ggaccagcca agatttcttt tttcttgata
     7681 tccactgtcg cgacagctaa tacaagacct tcttctgaaa gcaggtgacg gtcacgtaaa
     7741 acgacattac caatatcacc gatcccgcta ccatcgatat acacatctcc agccacaaaa
     7801 tgcccagcag gacgcgcaga atcgctagtt aacgctaaaa catcgccatt ttccatgatg
     7861 aagcagtttt ctgcaggaac acctacatct tgtgctagag aagcatgtac tttcaacatg
     7921 cggaattctc cgtggacagg aataaaatat tttggcttca tcaaacgaag cattaatttt
     7981 tgttcttctt gtccaccgtg accggaagta tggatgttat tgatttttcc gtgaatcact
     8041 tctgcaccag cttcagacag caaattgatc aaatgattga cacttgttgt attaccagga
     8101 ataggtgaac ttgaaaagat cactgtatcg tctggctgga tttggatttg acggtgagta
     8161 ccattcgcga tgcggctcaa cgcggccatc gcttctcctt gagaacctgt acataggatc
     8221 atcgtttcat tggcaggcaa gcgattgatt tcgtgtgcat ctacaaatgt gccgtctgga
     8281 actttgatat atcctaaacg ttgaccgttg acgatggcat tttccatgct tcgtccgaaa
     8341 acagcgattt tacgaccagt tgccacagcg gcatctgctg cttgctgcaa acggaagatg
     8401 ttagaagcaa agctggcaaa aatgatacgt ccttcgattt ttgaaaagat cttcaaaata
     8461 gaatgcccaa tcgttttttc tgatttagta aatgttggaa tctcagcatt tgtactatca
     8521 gataaaaggc acaagactcc ttcttccccg atacgagcca ttttatgcaa gtttgctggt
     8581 tcgccaacag gagtgaaatc gaatttgaag tctccagtcg ctacgatatt tccagaaggt
     8641 gttttcacca cgatccctaa tgcatctggg atactatggg tcgttctgaa aaagctgaca
     8701 gaagtctttc taaaacggat gatcgtatct tcattgatct catggagctc tgcatctcga
     8761 agcaagccat gctcatctaa tttgtttgtg atcaacgcta atgcgagagg accagcatag
     8821 ataggaatat ttgcttgttt cagcaagtag ggtattccgc cgatgtggtc ttcgtgcccg
     8881 tgagtaatca ccaaggcttt tactttgtgt aagttttgta tgatgtagct gtagtcggga
     8941 atgacatagt caattccaag taaatcgtct tccgggaatt tgatcccggc atcaatcaaa
     9001 atgatttcat cttggaattg taccccatac gtatttttcc cgatttcacc taatccacca
     9061 atagcaaaga cgcctgtctc gttatttttg atggaaagtt tcatattaaa actccactaa
     9121 tgagaaatca gcattttctt tttcgtactc tagatgactt ccttctagaa gttgaacgaa
     9181 ttcaatattg tatggggtat tttcttccac acgttggcga gcgtctacat cgctttttgc
     9241 ctcaatgtaa agagattttg tttcttcccg tttagggttg cgtacttttg tttcttgata
     9301 ataaacttta tagatcattg ttttgtctcc tttatctgcg taaagcagtg atttagctag
     9361 ttgatttttt tagacaacac aaaacgaggg gacaagccac ctggtttttc atatgctttg
     9421 actagaatac tggattcgag tcaaagagac aagcctcgtt tttgcttatc tgttcggata
     9481 ttttttcaac cgtaccttct gtataaaaaa tatacaacaa ttaattgtta ttctagcata
     9541 aaacagcaaa taagtataga aatcaactag attgaaagaa aaaatcaatg tttatcatcc
     9601 cgctataatc ataatgactt ttttatgaga gaagtgttct attaataggc tcgttgtgct
     9661 atcattttta gtaatgaaaa tgtaacaaaa gattaaaagg agatgagaaa gtgaaactaa
     9721 tgtggcatta taccatgcgt tataagaaat tcttgttttt caatttcata tgcgtatttg
     9781 gttttatttt gattgaatta ggacttccta cgattttagc acgtatgatc gatgtaggaa
     9841 tccgcaatga tgattttgat tatgtgaagc aacaagggat atttatgatc gttattactg
     9901 taatcggtat tattttgaat atcttgttag gttattttgg ttcgaggatt acgactaata
     9961 tcgtagcaga tattcgagat gatttgttcg aaaagatcca aagttattcg catcaggaat
    10021 acgaaaatct aggcgtatct tcattgatca ctcgtacaac aaacgatgct tatcagatca
    10081 tgttgttcct gcaaaatatt ttacgtatcg gttttatggc accaatgatg ttcattgtca
    10141 gtttgtacat ggtcatgcgt acaagtgtga cactcagttt gtatgtagta ggtgcattac
    10201 ctttactatt attggcagtc gtagcgattg ctaaggtgtc tgaaccgtta tctaaaaagc
    10261 aacaaaaaaa cttagataaa atcaacagta tcttgcggga aaatctttca ggattgcgtg
    10321 tgattcgtgc attcgtcaat gaaaagtttg aagaaaaacg ttttgctgta gtcaatgaga
    10381 attatgcttc tagttcaaaa agcttattcc gtttgatggc tgttgcacaa ccgggttttt
    10441 atttcttatt taatatcgta atggtattga tcatctggag cggggcagtg caaatcgatg
    10501 caggcgagct tcgtgtagga gatttgattg cgtttatcga gtatatattc catgccttgt
    10561 tctcattcat gttatttgct agtgtgttca tgatgtatcc aagagcagct gtttcagctg
    10621 gacgtatcca agaagcccta gatgccaaac cttcgattgt agaagatcca aatggtatat
    10681 ctgatacaaa aactaaaggg tatatcgaat ttcaaaatgt aacatttgct tatccaggcc
    10741 attcgcaaga gccagtgata cgaaatgtca gttttacagc ctctccagga gagaccgttg
    10801 cttttattgg aagtaccggt agcggaaaat ctacgttgat ccaattgatc cctcgctttt
    10861 atgatgtgag tgaaggaaag atcttgatcg atggagtaga cgtgagagat tatcggctaa
    10921 gcaagttgcg agataagatt gggtttattc cgcaaaaagc gctgctattt actggaacga
    10981 ttgcagataa tttgcgatac gggaaagaag atgctacgca agaagaaatg gaacgtgcag
    11041 ctgagattgc tcaagcttca gaattcattt ctcgaaaacc agatggattt aatgaacatt
    11101 tatcagaagg tggttccaat ttttcaggtg gacaaaaaca acgattggca atagccagag
    11161 ccatcatacg ccgtccggaa atctatattt ttgatgacag cttttctgca ttagattatc
    11221 aaacggatgc gaagttacgt gctcgtctga aaaaagaaac gacagaatct actgtattga
    11281 tcgtagcaca acgtgtggga acgatcatgc atgcagataa gatcatcgta ttaaatgaag
    11341 gagaagtcgt cggtatgggg acgcatcggg agttgttgga aaaatgcccg atttattatg
    11401 atattgccgc ttctcaatta tcaaaggagg aattagcatg aaaaaaacct tttcttcctt
    11461 aaaacgttta gctcgatata tcagaccgta tcgtgagact tttcttttag tgatcctatt
    11521 tacagttctg acagtggctt tcaacgtggc tttgccatat gttacaggac ttcctactac
    11581 agaaatcagc cgtaatattg ccaatggcga atcaatcaat ttttcttata tattcaaatg
    11641 tctcatttgg atcacagttg ttggaatagg gtattgtatt tcgcaattac tttctggtgt
    11701 tttgatgaca aatgtcgttc aaagtgccat gcatgatttg agaagagaca tagaagaaaa
    11761 aatcaatcgt cttccggttt catatttcga taaaaaccag caaggaaata ttttgtctcg
    11821 tgtgacgaat gatgtagatg cagtcagcgg tgctttacaa caagctttta ttggtatcgt
    11881 aaatgcgata ttgggaatta cgttggctgc agcaatgatg ttttatatcc agccgatgat
    11941 ggcgctgatc tctatgatca tgattccaac atcgatctgg atctcaaaac gagtgatcaa
    12001 tgcttcgcag aaatattttc aaagcatgca gaattctctt ggtgagctaa acggctacgt
    12061 ccaagaaaat atgacaggtt ttagtgtatt gaaagtatat ggacgcgaaa aagaaacatt
    12121 ggaaggattc aaaaaagtta atcacagctt gaaatacttt gggtttcgtg cagcattcat
    12181 ttctggcttg atgatgcctc tagttcaatt aactgcctat gctacttata ttggtatggc
    12241 tgttctagga agcttttatg ttattactgg ggcgatcgtt gtcggtcaac tacaagcatt
    12301 catccaatat atttggcaaa tcagtcagcc aatggggaac gtgacacaat tgtcttcagt
    12361 tttgcaaagt gcatcagcgg caacgaaacg tgtattcgag attttagatg agccagaaga
    12421 aaaagaaaac aaagtagatg cttcactgcc agaaacgatt ttaggagatg tgacattcga
    12481 tcatgtcgat tttgcatatg atccgaagaa accattaatc caagatttga attttgaagt
    12541 gaaagctggt caaaccgtag ctgtggtcgg tccgaccggt gccggcaaga cgacattgat
    12601 caatctattg atgcgttttt atgacgtaga caaaggagca ataaggattg atggaatcga
    12661 caccaaatca atgagtcgca gtgatgtgcg ttcgctattt ggtatggtct tgcaagatgc
    12721 atggctttat caaggaacga ttgcagataa tatccgcttc ggaaagcttg atgcgacaga
    12781 ttatgaagta gttgatgctg cgaaaacagc aaacgtagat cattttatcc gaacgatgcc
    12841 agatggatat gatatggcca tcaattctga gggagacaat gtgtctttag gacaaaaaca
    12901 gttgctgacg attgcccgag cagtggtctc cgatcctaaa atcttgattt tagatgaagc
    12961 aacaagttca gtagacaccc gtttagaagc gttgattcaa aaagcaatgg atcgcgtgat
    13021 ggagggacgg acaagtttcg tcatcgctca ccgtttatcg acaatccgtg atgcagatct
    13081 gattctagtg atggatcaag gtcagatcat tgaaaaagga acgcatgaga gtttattggc
    13141 acaaggtgga ttttatgaaa aactgtataa cagccaattt gctgaggaag cagaataagt
    13201 catataagaa taaaacagtt taaaacttat tttttgttct ctattcttga taatggtaaa
    13261 gtaaagttat caagaaaagt gaggacacgg agatggaaca gtttttaaat gaaaaaatta
    13321 ctgaagccaa aagagaagtt gatcgtttaa cgcacgtctg tcagacgaag ccttttgatt
    13381 cagctgattt ttttgaaaat gagtttcaaa tcgaacgctt ggcagcacaa atagaagtgt
    13441 acgaattaat tttaaaaaaa gtaatagaag aaaatataga tgtacaataa aaacaggtaa
    13501 aacgtcagtt accagatact gtaaatagac atctggcctg agcaggtttt acctgttttt
    13561 tttcttatga aataaatata tgctatgata cctttattca ataggattag tttctacaaa
    13621 aaatgaaagg agaaaaataa atatggaacg gtttatagca tcaaatgcca cagtgattgg
    13681 tgatgtgaca ttgtcagaag atgtgacgat ctggtatcaa gctgttttac gcggggacag
    13741 taattggata aaaataggac aaagaacgaa tatccaagat ggtacaatca tccatgtcga
    13801 tcatgatgca cctgtagata ttgcggagaa tgtgacggta ggacatcagt gcatgctaca
    13861 tggctgtaca atcgaaaaag gggcgctgat cgggatgggg accacgatat tgaatcatgc
    13921 ggttattggt gagaatagtt tgattggtgc tggatcatta gtaacagaag gaaaagttat
    13981 tcctcctaat gtcttggctt ttggtcgccc agctaaagtg atccgtcctt tgacaaaaga
    14041 agaaatacaa aaaaacaaag aaaacatcca gcattatgtc gagataggtc ggatgcatgc
    14101 gcaaggaaaa ttcaaggagc aaagataggc agtagcatcc ataaaataaa aaaggatggt
    14161 cagtttgagt tgaccatcct tttttatttg gtttagataa gagagcttaa aaaagcttcc
    14221 tttgaaacag attaatttaa tgaagaactg ttttccatga cttcatcgat caatccatat
    14281 gcttttgctt gttcagctgt catatagttg tcacgatctg tatctttttc aatcacttca
    14341 agcggttgtc cagtgcgttc agctaaaatt ttgtttaagc gttcgcgtgt ttgcaaaata
    14401 tggcgtgccg caatttcgat ttcagttgct tgcccttgag cgccgccaag aggttgatgg
    14461 atcatgatct cagcatttgg taaagcaaat cgtttacctt ttgtaccagc tgtcagcaag
    14521 aagcttccca ttgaagcagc catacccata acaattgttt ggacatctgc tttgacaaag
    14581 ttcattgtgt cgtaaatagc catacctgct gtaactgaac caccaggaga attgatataa
    14641 agatagatat ctttttcaga atcttgagcg tctaagaaaa gcaattgggc gatgatcgaa
    14701 ttagcaagat catctgttac ttggccgctt aacatgatga tacggtcttt caataaacgt
    14761 gagtagatat cgtaagctct ttcacctcgt gaagattgtt caatgactgt aggaattaaa
    14821 ttcataatag agttcctcct tgatttcgtt tagaaatagt gtaccataat ttagtttcta
    14881 atacctaaaa tgtagtatac atttttggtc aaaaaaggtc aaataaaaaa cttaggcaaa
    14941 ttagtcgcat aaacccgctt tatggtatga taagcagaga aacaaaacaa ggaggaacaa
    15001 cagattgata gttggtatta ctggccttat cggagtgctg acaggtgctg tagttgtcta
    15061 tatcggcatg atactgcaaa tgcgtcactt ggaaaataaa gaacagcgta gacgtgaatt
    15121 ggaattgaaa gtcaaagaaa tcgagacgct gaatcaatta aataaaaaga tcaacgagat
    15181 cttgcaaaaa cggacaacgc tgatggaaga atatgtcagc tttgatgcat ttgatgattg
    15241 ttatatcacg attgacgatt ttgcgtatct ccaatcattt tcagcgcaaa atagttttta
    15301 tctgccaaat tatattttag acgagctatt taagaatatc tcccatcgaa aagtgatttt
    15361 atctccagat gaaacaatga agattggtgg atatgcttat aaaggcggac gcgtgatttt
    15421 agagaatttt acggatagtc tgactgaaat gatcaatgaa aaaaaagctc agttgaagac
    15481 tatgacgcgc cagccgattg gtttcttctc gaaagatgat gtttggagct gatattttga
    15541 agcattgccg ttgcacatga gagcgtggta atgctttctt tttttcttaa tgatacaata
    15601 gaactgaacg gagtgaagaa agatgttaca aatctatgcc catcgcggaa gcagcggcac
    15661 ccacccagaa aatacgcttc ctgcatttgc agaagctgta cgagtaggtg cagatggtat
    15721 cgaactagat gtccatctaa gcaaagatgg ttatttgatc gttatgcacg acgaagaagt
    15781 ggaccggacg actaacggta aagggctgat tcgagaaaaa acattggaag aactcaaaaa
    15841 attgaatgca gggggcaaat tcagcaaaga atttccagca gctaaagttc ctgaattgag
    15901 agaagtgtta ggactgctcc tccaaaaaaa ttaccgaggg atgctaatga ttgaaattaa
    15961 aactgatcag tatgaatatc ccggtatcga agaaaaatta gccgaaacat taaaaagtag
    16021 agattggcct tttcaacatg catattgcag ctttcatttg gattctttgg aaaaaatcgc
    16081 attactggaa ccggagatac agctggactt attgatgagt acttctgata aaaagatcca
    16141 actagcagca gaaacccctt atatagaagg agtacatcca aaaaatactt ggttgttcga
    16201 acatccagtc caggcagctg attttccaaa agcaattcgt gcatggacga ttaacgatga
    16261 agaagaaatg gccaaatgtc aagaactcaa aatagatgga ataataactg attttccgga
    16321 gaaagctctt cggtggaaac aatcaacgaa aagtaggtaa aatatggaaa aagtattagt
    16381 gatcgtcggt ccgacagcag tcggtaaaac agcgctaagt gtggaactcg cacaactatt
    16441 tggaggagag atcatcagtg gtgattcatt gcaaatctat aaaaatctgg atatcggtac
    16501 agcaaaaatc aaagaagaag agatgaataa tgttccgcat catttgattg atgtgatcga
    16561 acctactcaa cgttactcag ccgttgattt tcagcattca ggtcgacaat tgattaccga
    16621 gattgcgaac cgagggcacc tacctatcat agctggtggt acgggtctct atatccaatc
    16681 ccttctatat gattatcagt taggagcgac agaagaagcg aatcctgaga ttagaaaaaa
    16741 gtatgaaacg ttagctgaaa caattggttc ccaagcatta tggaatcagt tgaaagaaaa
    16801 agacccggca gctgctgaga aaatccactg gaacaatcaa cgcaaagtga taagagctct
    16861 ggaagtttta gagacgacag gacatagtat cacggcacct aaagaagagc caaagcaatt
    16921 gtatgattac ctcatgattg gattagatac gaagcgtagt attctttatg aacgaatcaa
    16981 tcaaagagtc gatttgatgc tagcagaagg tttagtagca gaagcgcgta aggtgtatga
    17041 gcttggagaa gtccaggcca gtcaaggtat tggatataaa gaatttttcc cttattttga
    17101 agggaaagaa acattggaag aagcaacaga acaagtcaaa ctacattcga gaagatacgc
    17161 gaaacgtcag ttgacgtggt ttcgtaaccg tttggatgca cagtggtttg atcttataag
    17221 tgagccaaat caaaaaagtc agattgagca aacaatcaaa aaatggctgg aggcggaata
    17281 aaatggaaga aaaagtgatc atcgtagggg tagaaaccga agcaaaccag cgttactttt
    17341 cagagtcaat ggaagagcta gttcagctga cgaataccgc atcaggagaa gtcgtattca
    17401 caattacaca aaagagacca caagtggatc ggcaaacgat tatcggtaaa gggaaattac
    17461 aagaattagt tcaacaagcg gatgcgcatg aagcggattt gatcatcttt aatcatgagc
    17521 taactccgag acagagccaa ctgattaccg atgctgttgg tcttccggta attgatcgcg
    17581 tgcagcttat cttagatatt tttgcgatgc gtgctcgttc gaaagaaggg aagctgcagg
    17641 ttgaacttgc tcagttagaa tacctgctgc ctcgtttagt agggcaaggg aaaacattat
    17701 ctcgtcttgg cggtgggatc ggaacaagag ggccaggtga aacaaagtta gaaacagacc
    17761 gccgacatat acggaataaa atttttgctg ttaagaaaga attgaaagaa gtagaagctc
    17821 atcgagagcg caatcgccaa aaaagaaaaa atagcgagat ttttcagatt ggacttattg
    17881 gctacacgaa tgccgggaag tcaactatct tgaatctatt gacacaagcg gatacttatt
    17941 cgaaagatca attgtttgca acgttagatc cattgactaa gagatggcgt tttgctgaag
    18001 gatttgagat tactgtaacg gataccgtcg ggttcatcca agacctgcct acgcaactga
    18061 tcgatgcttt ccattctaca ttagaagaaa gtcaaaatat ggatcttttg ctacatgtag
    18121 tcgatgctag ttctccagat cgtattttac aggagcagac tgtcttaaaa ttgatggatg
    18181 aactgaatat gaaagaaatg ccgatactga ctgtttacaa taaggcagac cagatcgacc
    18241 cagcgatgtt cacgcctacg ctattcccaa atgtattgat ttctactcag acgaaagaag
    18301 gtaagatggc gttgatcgaa gcaatcaaac gtcagctgat ggaactaatg accccttata
    18361 cactttctgt gccaagcaac gaaggacaga aattaagcga gttgagaaga caaacaatgg
    18421 ttttggacga agcgtacatg gaagaaacaa acgaatatga agttaaaggg tttgcaaaaa
    18481 ccaattcaaa atggttaaga aaaaaccaat aaaattttat ttttttaa
//
LOCUS       DAZKOB010000053        17416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000053 DAZKOB010000000
VERSION     DAZKOB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.053"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..978
                     /locus_tag="ACG8M6_001718"
     CDS             289..978
                     /locus_tag="ACG8M6_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0457386.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            965..2173
                     /locus_tag="ACG8M6_001719"
     CDS             965..2173
                     /locus_tag="ACG8M6_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0457387.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2467..3738
                     /gene="serS"
                     /locus_tag="ACG8M6_001720"
     CDS             2467..3738
                     /gene="serS"
                     /locus_tag="ACG8M6_001720"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0457388.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4287..5771)
                     /gene="guaB"
                     /locus_tag="ACG8M6_001721"
     CDS             complement(4287..5771)
                     /gene="guaB"
                     /locus_tag="ACG8M6_001721"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0457389.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6276..6971)
                     /locus_tag="ACG8M6_001722"
     CDS             complement(6276..6971)
                     /locus_tag="ACG8M6_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0457390.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7061..8161)
                     /gene="ychF"
                     /locus_tag="ACG8M6_001723"
     CDS             complement(7061..8161)
                     /gene="ychF"
                     /locus_tag="ACG8M6_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0457391.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8170..8361)
                     /locus_tag="ACG8M6_001724"
     CDS             complement(8170..8361)
                     /locus_tag="ACG8M6_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0457392.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8382..9272)
                     /locus_tag="ACG8M6_001725"
     CDS             complement(8382..9272)
                     /locus_tag="ACG8M6_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0457393.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9259..10026)
                     /locus_tag="ACG8M6_001726"
     CDS             complement(9259..10026)
                     /locus_tag="ACG8M6_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0457394.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10038..10751)
                     /gene="rsmG"
                     /locus_tag="ACG8M6_001727"
     CDS             complement(10038..10751)
                     /gene="rsmG"
                     /locus_tag="ACG8M6_001727"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0457395.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10983..12884)
                     /gene="mnmG"
                     /locus_tag="ACG8M6_001728"
     CDS             complement(10983..12884)
                     /gene="mnmG"
                     /locus_tag="ACG8M6_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0457396.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12901..14298)
                     /gene="mnmE"
                     /locus_tag="ACG8M6_001729"
     CDS             complement(12901..14298)
                     /gene="mnmE"
                     /locus_tag="ACG8M6_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0457397.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14597..14980
                     /locus_tag="ACG8M6_001730"
     CDS             14597..14980
                     /locus_tag="ACG8M6_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457398.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15148..15906)
                     /gene="jag"
                     /locus_tag="ACG8M6_001731"
     CDS             complement(15148..15906)
                     /gene="jag"
                     /locus_tag="ACG8M6_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0457399.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15924..16742)
                     /locus_tag="ACG8M6_001732"
     CDS             complement(15924..16742)
                     /locus_tag="ACG8M6_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0457400.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16739..17095)
                     /gene="rnpA"
                     /locus_tag="ACG8M6_001733"
     CDS             complement(16739..17095)
                     /gene="rnpA"
                     /locus_tag="ACG8M6_001733"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0457401.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17213..17347)
                     /gene="rpmH"
                     /locus_tag="ACG8M6_001734"
     CDS             complement(17213..17347)
                     /gene="rpmH"
                     /locus_tag="ACG8M6_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0457402.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
BASE COUNT         4848 a         3636 c         3190 g         5742 t
ORIGIN      
        1 ataataatat atcaacatca catgtgaaag tcaataagaa aatgcttatt tatcaacatt
       61 tctagcttac atgatcattg gttagttctg gtcctttttc gttcgtcttt ataactccga
      121 gagacgtaaa atccttttgt tttcagttta atgttcatgt tcttgtctta ttcacaccaa
      181 ctagatgtct caccttctaa aattttcctt aacaaagtgc tgcttttatc tatttctgct
      241 tttttttgct agaataatag taacaatatg actctggagg atttatttat gaaaattttg
      301 attgctgacg atgataaaga aatcgttgaa ttattaagta tctatgttca caatgagggg
      361 tatgaagcag taaaggctta tgatgggaaa gaagcattat ctaaaatcag aacaatccct
      421 gatattgaat tacttatttt agatatcatg atgcctgaga tggatgggat gcaagtagtg
      481 aaagagctac gaaaagaatc ccaaatacca atcattatgc tgacagcaaa aactactgat
      541 atggataaga tcaaaggact ggtcgctggt gcagatgatt atgtgaccaa gccatttaat
      601 ccgttagaag taatggctag agtaaagtct ttattacggc gtacaaaaat gcagattgcc
      661 ccagatcagc ctgatgtatt agaagtaaat gcattaatca tcaataaaga ctctcatgaa
      721 gttaaaacaa tcgatgggaa agaaatccag ttaacagctt tggaattcgg tatcctctac
      781 ttgcttgcta gtcatcctaa tcgagtattt agcgcggatg aaatttttga gcgtgtttgg
      841 aaacaagaaa gtattgtctc tgctaaaact gtcatggttc acgttagtca tttaagagat
      901 aagattgaag aagcaactgg tggtgaaaaa gtcattcaaa ccgtttgggg agtgggctat
      961 aaaattgacg caagatagta aacgagcaac tgaaaaacgc cgtcggatca atttgacttc
     1021 aaaagaaatc agcgaattgt tagcggaagg aatcatcacg attatccttt tgcttctatt
     1081 aaatgtttct attctggttg ttatcagttc ggtgatcaac agttcacctt ctttgacgaa
     1141 tgcgatttgg gattcaaaga atatttttgc agaacgattg aacactgatt tgttctggaa
     1201 cggccggaat tttattattc cctttttctt tctattagat attggtgttt tgtattggcg
     1261 cttgattcga agatatcgcc aaatgcaatt gcggcatatc atcagcgaac ttcactacat
     1321 cgcaaacgga aattatgatc atcgtatccc ttttgaactg agtggtgact taagccgagt
     1381 ggtaacaagt atcaatggat tggtggatag taccgtagca gcgattgaag acgaacgtaa
     1441 aatcgaaaaa tcaaaagatg aattgatcac gaatgtcagt catgatatcc gtaccccact
     1501 cacgtccatc attggatacc taggactgat agaagatggc cagtatcact cagaagaaga
     1561 tcttttaaag tatacgcata ctgcttatat caaagcaaag cagatgaaat ctttagtgga
     1621 cgacttattt gaatacacta aggtacgcca gcctgctgtc ccggtgaatt tttctgcctt
     1681 tgacatgatc cagttgatcg aacagttagc agctgatttt gagttggaag cttctaaaaa
     1741 aaatatccaa atacttgttc aatcaaaagt agactccctt atcatggatg gcgataccga
     1801 aaaacttgta cgtgtcttca ataatctttt gacgaatgcg ttgaaatacg gaaaaggagc
     1861 aacgaagatc gtaatcgaag tagaacgtat cggttcagaa gttgttgcaa cagtcaaaaa
     1921 taacggagca atgatcccac aacaggcaat cgataatcta ttcgaccgtt tttatcgtgt
     1981 agaagaatct cgttcacaag caactggagg aactggactt ggtttagcaa tcgctcaaag
     2041 cattgtggct ttgcacggcg gatacattta tgcaaaatca gataagcaat ggacttcttt
     2101 catcatgcac cttccaatca agaaaaatga aaaattacct attgatacac aggaagttat
     2161 tgacgaaagc tgaaatattc gctattctta ttacaattat tagaataaat gaaaaacaca
     2221 gaacccagta cactttattc tttttcagag agctgatggc aggtgtaaat cagcaaagga
     2281 taatttggaa gttccagttc gtttacgtgc cctttcgaaa taagaaaggt cggttcaaca
     2341 gccgttatgt gttcaacaag tgcagctgac gctgaacttg ggtggtaccg cgaatttcag
     2401 actgattcgt cccaagaata atgggatgga tcagtctttt tttattttca aaaaggagga
     2461 aatcatatgt tagatgtaaa aatgatgcga caaaatattg aagaagtaca agaaaaattg
     2521 atgactcggg gcgtaaaaaa agaaacatta gctgaattca tggaactaga tgaaaatcgt
     2581 cgtaagctac tagtcaaaag tgaagaatta aaaaaatatc gaaatgatgt ttctgctgag
     2641 atcgcccaat tgaagcgtgc aaaagaagat gcttctgcta aaatcgctga aatgaaagaa
     2701 gtcggtggca acatcaaagc attggatact gaaattgctg aagtggatga aaaattgaaa
     2761 caaattgcaa ctactttgcc aaaccttccc catgattctg tgccagttgg aacagatgaa
     2821 gaggacaatg tagaaatccg tcgttggagc gaaccacgta catttgcttt tgaaccaaaa
     2881 ccacactggg aaattgcaga aaatcttgat attttagatt ttgaacgtgg cgcaaaagta
     2941 gccggtagcc gttttgtcta ttacaaagga ttaggtgctc gattagaacg tgctgtctac
     3001 aacttcatgc ttgatcagca tatttacgaa cacggctata ctgaaatgat cactccttat
     3061 atcgtcaaca gtaaagcgat gtttggtacg ggacaatttc caaaattcaa agaagatgtt
     3121 ttccaattgg agaatactga tctaacatta atcccaacag ctgaagtacc attaactaac
     3181 tattataatg atgaaatttt agatggtgca gatttaccta tttatttcac tgcactaagc
     3241 cctgcttttc gttctgaagc cggaagtgct ggtcgggata cacgaggact gattcgtctt
     3301 caccaattca ataaagtaga aatggtcaaa ttttcggatg tagagcattc ttacgaagaa
     3361 ttggaaaaaa tgacagctga tgcagaagat atcttgaaaa aattgcattt gccttatcgt
     3421 gtgatggcct tatcaactgg agacatgggc ttttctgcag ctaaaactta tgacttagaa
     3481 gtatggatac ctgcacaaga tacttatcgt gaaatcagtt cttgttctaa ctgtgaagat
     3541 ttccaagcac gccgcgcaat gatccgttat cgcgataatg atggaaaaat ccactatact
     3601 catactctga atggttcagg acttgcagtt ggccgtacag tagctgccat tttggaaaac
     3661 taccaaaatg aagatggttc tgtcacagtt ccagaagcgc tcgttcctta tatggggaat
     3721 ctgaaagtta ttaaataaag aggctgtgaa aaagccgctt agtttcgagt attaagaaaa
     3781 attttgtgaa aatggctttc cacatttttg cataaattgg acttaatacc gagaaactgg
     3841 cttttgaaca ccatttatcg agaggttgtg aaatcagcga attaacctga gtattagaga
     3901 aaaaaatgga aaaatagtcc ctcatatttt accgtttgtt gaactaatac cgagggtcag
     3961 ctgattgaac accgtttagt ggagaggtcg taagcctacc ccccagctcc tagttacaag
     4021 gacaaattca aaaaacagct cttcatattt tatgaatgtt gtacttgtga ttcggaggtg
     4081 gcaagggaac actgtttatt caaaaagagg ctgggacaga agcgtttaac tccaagaaat
     4141 aagaagaaat tcacgaaaat tgcttttcaa atttttgtga atttcttctt atttccgaag
     4201 gagttgcttc tacttccgcc gtttatacgt gtttagagcg tgagacaaaa gtgttcttta
     4261 cttttgtccc acgctcttta atgtttttaa gattcaactg aatagtttgg tgcttctttt
     4321 gtgatttgta catcatgagg gtgtgattct tttaatccac tgccgctcat ttggataaat
     4381 tgcgcttcat cacgtaattg ctgtagattg gctgccccaa cataacccat tcctgaacgc
     4441 aaaccgccga tcatttggaa aataatatct gcaacacttc ctttataagc aacccgacct
     4501 tcgattcctt ctggtactaa tttatttgct tcattgacac taccttggaa gtaacggtct
     4561 tttgaacctt tttccatcgc gcctaatgag cccatacctc ggtatgtttt gaatcgacgt
     4621 ccttggaaga tttcgaattc acctggtgat tcatctgtcc ctgccaacat acttcctagc
     4681 atcactgcat gtccgccggc agctaatgct ttgacgatgt caccagaata tttgatccct
     4741 ccgtctgcaa taatcgcttt gccatattga cgagcaactg atgctgcatc ataaatagct
     4801 gtgagctgag gtacacctac accggctacc acacgtgtcg tacaaatcga tcctggtccg
     4861 ataccaactt ttacgacatc tacacctgca tcatataaag cttttgtcgc ttcagccgtc
     4921 gcaacgtttc ctgcaatcaa tgttgcatct gcaaaagttg aacggatttc ttgaattttt
     4981 cggatcacac ctgcactatg tccatgagct gtatcgatga tgattgcatc tgcccctgct
     5041 tctaataaag cattcgcacg ctcaaatgta tcgcttgtta caccaacggc tgctgctaca
     5101 agcaatcgac catgttcatc ttttgctgca tttggaaatt cgatcacttt ctcgatgtct
     5161 ttgatcgtga tcaatccgct taaacgacct tcgttatcta cgattggtaa tttttcgatt
     5221 ttatgttttt gcaaaatctt ttctgcatct tttagagagg tacctactgg ggcagtcact
     5281 aagttgtctt ttgtcatcac atcagcaatc ggcatgtgat aatctgttac aaaacgcatg
     5341 tcgcggtttg taattatccc taccaatttt ctgttttcaa gtgtttcaac gattggcaca
     5401 ccactgatac gatatttgct catcaattct tctgcgtctg ctacaagatg ctgtggcgtt
     5461 aagaagaacg gatcaatgat tacgccactt tcagaacgtt tgacttttct tacttcatcc
     5521 gcttgctggc tgatcgtcat gtttttatgg atcacaccta gtccgccttg tcttgccata
     5581 gcaatggcca tcttgctgtc ggtaacagta tccatacttg cactgatgat tggaatattc
     5641 aacttaatat ttttagctaa ttgtacgctc atatccactt cgtttggtaa cacgtgactt
     5701 tctgctggta tcaataagac atcatcaaaa gtaaatcctt ttttcgtgaa ctttgtttcc
     5761 cagttggaca tttttagtac cactcctcta atttagttta tctattaaaa taattgattt
     5821 tttcatttaa gtcaatgatc tacttggaat caagttagct ttctcataga ataccaatat
     5881 aaagcgttta catctgtgtg tattaaaaga gattgtaaaa aagctgtcct gcatcaagta
     5941 ataagaacag ccaatcaaaa acagcttctc atgtttttga attggttggg tttattaccg
     6001 cagatgcaaa cttttgaaca ccgtttagtg gagaggctgt gaaaaagctg cttagtttcg
     6061 agtagtaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat
     6121 accgagaaac tggcttttga acaccgttta tcgtaaaaag ggactgtgac aagacttttg
     6181 tcacagtccc ttactattat ttaactatgg ataaacaaaa tgaggttctt tactacccct
     6241 agatatctag caaaatagct gatctttttg ctagtttatc ttcttgggac ggataaactt
     6301 ccaataatcc cgtatttttt cttcaagtag taacggattg ctaaagcagt tgccccgata
     6361 atgataacta ctactggatc taatactgga ttgataactg gtggcaatag tgttgtcgca
     6421 gaaaacagta cgacccaaag aagtgtcgtt ccacctagga taagcaatgt cttacccatt
     6481 cctggtttct tgcttcgatc tgctccggga tattcatatt gatagatgta tttgtacatc
     6541 atataaaaga cccagccacc gattgcagaa cctagaacga gtgtagaaaa gccataagct
     6601 ggcgttgttc cacgtgaaaa caatccggta atcgcaatca tgatacttaa tagaccaagc
     6661 aacagcaacg tattatctaa ccacatcaat aaaggggtgg atgaattttt tacttctgct
     6721 ggcttattga tgattgcttc cgttctttca gagactgtcc caaacaattg acgggctgtt
     6781 cttccgcctt tttgttctgc aactaaagta ggcagaatgt catgaagggc ttttactttt
     6841 tcttcttcag acaaattcgc tgcttctaaa gattttttca aatcaaaaat gtactgttga
     6901 tttcttttgg ttaattttgt ttctaaacta ttgttttctt ttacgattgc tcgtaattgt
     6961 tctggttcca ttatctaata gtcctcctta gccgttcctt ttctcaacgg tcgtcacaaa
     7021 agacattcta ccatattcta cagctagttt gatagccctt ttacacatta aagcggaata
     7081 acattacgtc gccgtcttgt acgacataat cttttccttc taatcgaaca cgtccagctt
     7141 cttttgctgc atgcatgttt ccgtaatgat ccaagtcttc aaaagagaca gtttctgcac
     7201 ggataaatcc gcgttcgaaa tctgaatgga tgattcctgc tgcttgtggt gctttgattc
     7261 cttttttgaa tgtccaagca cgtacttctt gttccccagc agtaaagtaa gtagctaagc
     7321 ctaacaaatc ataagcagca cggatcaact gatccaaacc tgattcttcg attcctaaag
     7381 cttctaaaaa ttccgctttg tcttcttcat ctaattctgc gatttcttct tctgcacgag
     7441 cactgaccac gatcacttct gcattttctt ctgcagcaaa gttgcgcact tcttgtacat
     7501 aatgattcgc gtctgcatct gctacgtcat cttctgagac attggctaca taaagaattg
     7561 gttttgtcgt caataaaaat agtgatttaa cgattttttg ttcttcttca gtaaattcga
     7621 tcgttcgggc agattttcct tcttccagta caggtttgat tttatccagt acagctagtt
     7681 ctgccactgc atctttatct ttggttttag caacttttgc tacacgagta tagcgtttgt
     7741 tgatagattc taagtctgct aacactagtt ccaagttgat cgtatcaata tcttctagtg
     7801 gatcaacacg tccttctacg tgagtaatat tatcatcatc aaaacagcga acaacgtgac
     7861 aaatagcgtc tacttggcgg atatggctta agaattgatt tcccaaacct tctcctttac
     7921 ttgcgccttt tacgattcca gcaatatctg taaattcaaa agtcgtcgga acagtttttt
     7981 tcggatgtac aagttctgtt aagcgttgca aacgccagtc aggtacttct accatcccta
     8041 cgttggggtc gatcgtcgca aagggatagt ttgctgcttc tgctcctgct tttgtgatcg
     8101 cattaaacaa agttgatttt cctacgttgg gcaacccaac aattccagca gtcaatgcca
     8161 taaatttatt cactctttct ctgcattttt ttcaattact ttttttaatt tcttttcaaa
     8221 atctcgacgg gtcatcatga cgatatgtcc gcaattcata catctgattt tgatatctgc
     8281 gcccattcga atgacttccc agcgattcgt ctggcaagcg tgaggttttt tcatttcaac
     8341 gatatcgtgc aagtcgtaca taaaaatagc ctcctttttg tttattcttc atcaaattga
     8401 atattcaaga tatcaagaat tcgagtcaaa tctttttgag aaagatattc aatctcgatc
     8461 ttacctttcc catttttttc ttggatagct acacttgtac caaatttatc catcaaacga
     8521 tcttcgcttt ctcggatata atatggtttt tcctttgcta gccttgggat ttttttcttt
     8581 tcttttccat cattttgatt taactcagaa acaagctgtt ccagttggcg gacagttaaa
     8641 ttttcagtta ctgcacgatt ggctaatttc aagatcaatg ttttattttt taaacctaac
     8701 agcgtacggg cttgtcccat agataaacgc tgatcttgta ccatttcctt gacaagatcc
     8761 ggtaaagaaa gcaagcgcaa ataattggcg atataaggtc tgctctttcc taaacgacta
     8821 gctacttctg cttgtgttag tttcaggttt ttcatcagca tctcatatgc ttctgcttct
     8881 tctaacggat tcagatcttc tcgctgcaag ttttccagca ctgctacttg catcatcgct
     8941 tcttcgtcaa agtctctaac gatagcaggg atcgtttctt tttcagctag tttggacgct
     9001 cgaaaacgtc gctcccctgc aataatctca tatcctttta ctgcggaacg gcgtacgatg
     9061 atgggttgaa agacacctga ttgttcaata gaattagcta gttcctgcaa agatacttct
     9121 tcaaatgttt tacggggctg atacggattt ggtcgcagct cattcaatgg tagctggaca
     9181 acggtatcat cttttacatc caccgtctct aagttttcta aatcttgaaa cagtgcatcg
     9241 attcctcggc ctaatccttt acttttcttc acgacttacc acttcctttg ccaatgcttg
     9301 gtagacttca gctcctcgag aacgtggatc gtagtcgatg atcggttttc catgactagg
     9361 tgcttctgat aaacgaatat ttcttggaat gatcgtttcg taaacttttt cgcggaagta
     9421 tttacgtact tcttctacga cttcatttcc cagatttgtt cgtgcatcat acattgtcag
     9481 taagactccc tcgatctcta attctggatt aaagtgtttt tggactaaac gaaccgtatt
     9541 cagtaattgg cttaatcctt ccaatgcgta atactcacac tgaacaggta tcaaaatcga
     9601 atcacttgct gtaaatgaat tgattgtcag atggccaaga gaaggcgggc aatcgatcaa
     9661 aatataatca tactgactgc ttacttctgc taaagatcct tttaatcttg attctcttgc
     9721 catcattgac gtcaattcga tctctgctcc tgctaattgg agagtcgcag gcacaatact
     9781 caaattttcg tgttcagtga tcaaagttgc ctcatcaatt ggcaattcat tgactagaac
     9841 atcatagatg tctcttgtca catcaggttt tctgatcccg acaccactgg tagcatttcc
     9901 ttgtgcatcc atatccacta gcaatacttt tttgccaagg ctagcaagac aagcacctag
     9961 attcaccgtc gtcgttgttt tgccgacgcc acctttttgg ttcgcaacag atattatttg
    10021 tgccatctct tctcctccta tttgattggc tgcttattag gcagtccggg ctttcttggg
    10081 tatttctttg gggttggttt ctttttttga ataacgatga tatgccgttg atcagagaca
    10141 ttcggcaagg taaccgcctc ttctttgagg aatttaccac ctaataaagc aatcgcttgt
    10201 tttgcttctg tcaattcctc ttcacttttt gctgctttca atgcataaaa atagccttct
    10261 tgtttagcca agggcaaaca caattccgct aacacattca aacgagcaac cgcacgagca
    10321 gtgacaaggt caaatgatgc acgaaagtga ggattttgcc caaatgtttc tgcccggtca
    10381 tgatataagg aagcatcgat tcctaataat cgggttaact cagataagaa ggttattcgt
    10441 ttattcagag aatcaacgat cgttatctgc agttctggaa aggcaatttt taaaggtatg
    10501 ctaggaaacc ctgcccccga accaacatcg cataaagata aaggttgcgt aaagtctact
    10561 tcaaatgcta atgtaagtga atcgtaaaaa tgcttcagat atacgtcttt ttcatccgta
    10621 atagctgtaa ggttcatctt ttcgttccat tcgactaatg tccgataata aatagcgaat
    10681 tgattcattt gatgatcggt aagctctatc cctttttctg ccaaggattg gcgaaattct
    10741 tctggtatca taaatattcc tttcattgtg aaacagcaat tgtttcacag attctctccc
    10801 tactttaacg caaaatctct agacatgcaa tccttccata tggattctta aaaatttgaa
    10861 aagtaagaaa agccgataga ctttttcttt ctagtatgac tcataaaata tacacagaag
    10921 agagaactag ctttgagcaa tttctgcaaa aactagttct ctcttctatt tattattacg
    10981 tattacgctg gttcactgac tttcgcaatc ttcccttgtt cgatatagac cattaggata
    11041 cttaggtctg cagggtttac gccactgatt cggctagctt gggcaattgt ttctggttgg
    11101 attttctgca atttttgttt tgcttctgtt gctaatccgt tgattgctgc gtagtcgatg
    11161 ttttctggaa tctttttcgc ttccatgcgt tttaattttt ctactttttc catggctttt
    11221 ttgatgtagc cttcatattt gatttggatt tctacttgtt cgatttcttt tcgtgtcaat
    11281 tcttcatttt ctggaataaa ccgtagaagt tctgcataag taatctcagg acgtcttaaa
    11341 aagtcgcttg ctagtacacc atcttttaat ggtgccgcat taatcgttgc taaaaagtct
    11401 tggacttctt ctgttggttt gattcgtaca ttcatcaaac gtttgatttc ttgttcaact
    11461 gcttcttttt tcttcaaata attcttgtat tgatcttctt ttactaagcc tacacggtga
    11521 cccatttctg ttaatcgcaa atctgcatta tcatgacgta aaagcagacg gtattcagca
    11581 cgtgaagtca gcaaacgata aggttcgttc gttccttttg ttaccaaatc atcgatcatg
    11641 acaccgatgt aaccgtcact tcgtttgatc actaaaggtt cttttccctg aactttcaaa
    11701 gctgcattga taccggcaat cagcccttgt ccagccgctt cttcataacc gcttgtacca
    11761 tttgtttgtc cagctgtata aagattttca acgactttag tttctaatgt cggacggagc
    11821 tggtgtggct ctactacgtc atattcgata gcatatcctg tacgcatcat ttctactttt
    11881 tctaatccag caatagaatg aagcatgttg acttgaacat cctctggtaa agaagtagat
    11941 aaaccttgaa catagacttc ttccgtattt aagccttctg gttccaaaaa taattgatga
    12001 cgtggtttgt ctgcaaaacg cacgatctta tcttcgatgg atggacaata gcgagcgcct
    12061 accccttcaa cgatccctgt aaacattggg gcacgatgta agttgtctcg aataatttgg
    12121 tgagtcgtct cacctgtata ggtcagccag caagattcct gatctagttt ataggcacta
    12181 tctggtgtgc tgaagctaaa atgattcggc tctttgtcac caggctgctc ttctgtaaca
    12241 gaatagtcga tcgtacttga ttttactcga ggcggtgttc ctgttttgaa ccgttcgata
    12301 gcaaagccaa gttcttttag atgattcgct agtccaatag aaggttgaga gttattcgga
    12361 cctgatgaat atttcaattc accgataatg atttctccgc gaagtgctgt tccggctgta
    12421 ataacaactg ctttggcccg gtagctcgct cctgtggacg tcactactcc atgacaaaca
    12481 ccatcttcga cgatcagttc ttcgacgatt ccttgtctta gcgtcaaatt tggttctttt
    12541 tcgatcgtat gtttcatttc tgttgaataa gcatgtttgt ctgcttgtgc tcgcaatgct
    12601 cggacagctg ggccttttcc tgtgttcaac atacgcattt ggatataggt tttatcgata
    12661 ttcttaccca tttctccgcc taacgcatcg atttctcgaa cgacaacgcc tttagctggt
    12721 ccgcctacag aaggattaca tggcatgaat gctaccatgt ccaaatttat tgtcagtaat
    12781 aatgtttttg atcccatacg tgcagcagca agtgcagctt ctgaaccggc gtgtcctgcg
    12841 ccaacaacga tcacgtcata cgcatctgct tcataatgtt ccaatcgttt gttcctcctt
    12901 ttattttcct aaacaaaatt gactgaataa ttgagtgatt agctcatctt gtacactgtc
    12961 gccgacaact tctcctagat agtcccagca acgcgtcata tctatttgaa cgagatccac
    13021 aggcattcct gaatcaatac ctgcaatgac ttcttcaagt gagagggaag ctttttctaa
    13081 caacgcgata tggcgggtat tagaaacata agtagcgtct ctttctcctg tttctccgct
    13141 gaagaataaa tcagaaatag ctgattccaa acgatctagt ccgtcgtttt tagctacgga
    13201 aattgaaaaa actggttcat tttcgatcaa tttcttcaat ttttcttgtt ccagctgtgc
    13261 tggcaaatct gttttattta gaagaatgat ccgtttcaat ccacttgtcg cttccaataa
    13321 ttgttcatct tctgctgtca gtggttcgct ttggtttaga acaagtaaga tcaagtctgc
    13381 ttctgctaat gcttttcggc ttctttcgac cccgatccgt tccaccacat cttctgtttc
    13441 ccgaattcca gctgtatcga ttagcttgag cggtaccccc cgaacattga catactcttc
    13501 gattacatct cgagtcgttc ctgcaatatc cgtaacgatt gctttttctt ctcttaacaa
    13561 atggtttaac aaacttgatt tgccgacatt tggccggccg atgattgccg tacttaaccc
    13621 ttcccgcaga accttccctt gcttggatgt agcaagtagc gcttggattc tttgttggat
    13681 catctgcgct ttttcaagca ataacttcgt agtcaattct tcgacatcat catattctgg
    13741 ataatcaata tttacttcta cttgtgctaa tgtctctaaa atctcttgtc tcaatgagcg
    13801 gatcaaagca gacagattgc cgtctaactg attaagagct agtcccattg ctttgtctgt
    13861 ttttgcccga atcaaatcca tcacagcttc tgcctgtgaa aggtcaacac gaccattcaa
    13921 gaatgctcgt tttgtgaatt cgcctggctc agctaatctt gcaccttctc ttaacaatag
    13981 ctgcaaaatt tgattgacta caacgatccc accgtgacaa ttgatttcta ctacatcttc
    14041 ccgcgtaaat gtctttggtg ctctcatcac ggagaccatg acttcatcga caagctgatt
    14101 gctcttggga tcaacgatat gtccataatg gatcgtatga cttggtactt ctgataatcg
    14161 tttgtttcca gattgataaa ctttatctgc tagttgcaca gcttgatcgc cacttaaacg
    14221 aacgatactg attgcacctt ctccaggtgg ggtagaaatt gccgcaatcg tatcaaattc
    14281 taaagtaatt tcagccattt tttcacttcc tttttcattt tgcgcacaaa aaaagtgccc
    14341 actccaccct ttatgcacaa taaacggtga acttcgcact ttgactgctt gttcttgatt
    14401 aaattctggt catagattat cacacttgct ataaggaatc aagtgtttaa aacaattaga
    14461 tgaagttgag cctgttctgc ttccattacc ttatatttaa ttgtccttgg atgtctcttt
    14521 gcgttctttg gtttattcga agagaagata ctgtataaac aaggctttat ctttcttaaa
    14581 aaaaacggta cactaaatgg tagcgctatc taaactatac acttttgaaa ggagaaaata
    14641 tgtgagcccg ttcagatctg ctgcattgga agaagaaagt gaccctcgga tcaacgatgt
    14701 cgttttatct acagggaata tcaaccagaa atatatcgtc cgcgatatcg tttttgttgt
    14761 ggaatgctta gaagctgatt tgtttgaccc atcttttccc aaagatgacc ttttcttaac
    14821 aacgaagagg aaattaaaga cgaaggtact tgaatatggt gcgaatgcag taatcaactg
    14881 tcattttgag tatcagcaaa aaaaacgcga cggaaagtat tttctggaac tttttgctta
    14941 tggaacagtg atccaattta tccaaacaac gattggttaa atgaaacaga caaaaaaagc
    15001 ggatcaaaac tgaagacagt ttcgatccgc ttttttctat caaagttagt gactacttca
    15061 tcgtatactc tgataagaat gatttctagt aaaatatact tggtttgaaa aactttgtca
    15121 acgtgattta ttcaaaaagt aatggaatca ataatatttt ttagcaggtt ctactactaa
    15181 ataacgataa ggttcctcac cttccgaatg tgtttttaca tgttcatctt tgcttaatgc
    15241 agcatggatt tgtttacgtt caaatgcagg cattggttct aagaaaacag gctgccctgt
    15301 ccgttttact ttttcagccg tgcgatcagc taaacgctga atgatctctt ggcgcttttc
    15361 acggtaattg cctacattga ctactacaga cagcttattt cttgctaaac gatggatgaa
    15421 gacttgtgcc agatattgca atgcattcag tgtttttcca tgtttcccaa tcagtatgcc
    15481 ttgcttttgt gtttctaaat ggaaaacaac catattattt gattctcttt ccattttgac
    15541 aagagctgga gcatcaagct gttttgaaat ttcagtaaga taaactgcca attccgttaa
    15601 agctgcttca tcacctagct cacttacttc ttctgttttt tcttcaagaa tcgtttcgtt
    15661 gactacctcg atctcagctg ttggttcttc ttccaaaatt tcttctacag tctgttctac
    15721 tgtttcagaa actttttcgc tgatacttgg ctggatcgaa agccgtgcat tctttcgacc
    15781 cattcctagg aaaccttttt ttccttcttc cagtatttcg atttcgactt ggtctttggc
    15841 caaacccaaa tcagccagac ctagttggac agcttcgtct attgttgcag cttcataaat
    15901 cggcattagc attacctccc ctattatttc cgttttttct taggattcat tgctttctta
    15961 agagcacgtt ctctttcgcg gattctccgt gcttcttctt cacgctcttt tcgaattttg
    16021 aaagggttgt tgatcagcag cgtttgtaca acttggaagg cattggaaac tacccagtat
    16081 aatgaaagac cgctggctaa attgacaccc atcactaaaa tcattacagg catcacgaaa
    16141 ttcatgatct tcattgatgc attcgattca atttgactca tgctagataa atacgtactt
    16201 gcaaaagtaa agattgctgc taaaatcggc aaaataaatg ttggatccgc gttccctaag
    16261 ttcaaccata agaaatgccc ttgtttcaac tctggtacac gtgaaataga ttgccataac
    16321 gccatcaaga ttggcatttg tactaataaa ggaagacaac cagcatatgg attgacgcca
    16381 ttttctgcat ataaacgctg ctgctcttct cttaacttac tttgtgtttc cggatctctt
    16441 gaactatatt gttgctgcag tgcttttaat ttcggttgta attcttgcgt tttacgcatg
    16501 ctctttgttt ggaaatgcat caacggcaat aggatcacgc ggatgatcag tgtaaataaa
    16561 atgatcccta tccctgcatt accaaatgac aacgccttga tcgcctcagc aaagtaataa
    16621 acaatataac gatcccaaat tcctgtactc tgtgcgctga cttccccagt tccgcaaccg
    16681 gataagacga tgaccaaact aacgataccg gccatcaaaa gtaaacgttt gtatttcttc
    16741 acttactctt ttcctctctg tcgattattt ttgcaagttt taagacgtgg actaaattgg
    16801 agcgaacctc atcataagtc aaccccttca cacttggtct agcaatcaca ataaaatcca
    16861 actccggatc aatttcttct tttatagaat aaactgctgc tcggattttt cgtttgactt
    16921 cattgcggtt taccgcgttg ccgacctttt ttcctacaga taaaccaact cgaaaatgag
    16981 gctgtcccgg tttttccaaa cggtaaacga cgaatttgcg gttagcaaaa gaatctcccg
    17041 tatggaacac ggcttgaaac tctcgttctt tttttactcg atacgctttt ctcattagtt
    17101 ttctttcctt ttctatctct cacttacgct cataatcata ccataattac gattgtcctt
    17161 tcatcatttc cctaaaaaag agtataaaaa aaaccactga aaccccagtg gcctaagctg
    17221 aaagaacttt tcttcctttg cgacgacggc tagctaacac acgacgtcca tttttagtac
    17281 tcatacgttt acggaatccg tgaacttttt gacgtttacg tttatttggt tggtatgttc
    17341 ttttcatata ttccacctcc aaaatgttct tatctatttt ataaacacag acatactttg
    17401 atagtataac gacaaa
//
LOCUS       DAZKOB010000054        16923 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000054 DAZKOB010000000
VERSION     DAZKOB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.054"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACG8M6_001735"
     CDS             288..695
                     /locus_tag="ACG8M6_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457403.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACG8M6_001736"
     CDS             709..1110
                     /locus_tag="ACG8M6_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457404.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="ACG8M6_001737"
     CDS             1112..1483
                     /locus_tag="ACG8M6_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457405.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1818)
                     /locus_tag="ACG8M6_001738"
     CDS             complement(1519..1818)
                     /locus_tag="ACG8M6_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0457406.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1824..2054)
                     /locus_tag="ACG8M6_001739"
     CDS             complement(1824..2054)
                     /locus_tag="ACG8M6_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457407.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(2490..3056)
                     /locus_tag="ACG8M6_001740"
     CDS             complement(2490..3056)
                     /locus_tag="ACG8M6_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0457408.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(3056..4012)
                     /locus_tag="ACG8M6_001741"
     CDS             complement(3056..4012)
                     /locus_tag="ACG8M6_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0457409.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(4012..4668)
                     /locus_tag="ACG8M6_001742"
     CDS             complement(4012..4668)
                     /locus_tag="ACG8M6_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0457410.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            5038..7056
                     /locus_tag="ACG8M6_001743"
     CDS             5038..7056
                     /locus_tag="ACG8M6_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0457411.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    7126..7332
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7483..9897
                     /gene="leuS"
                     /locus_tag="ACG8M6_001744"
     CDS             7483..9897
                     /gene="leuS"
                     /locus_tag="ACG8M6_001744"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0457412.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            10245..11798
                     /locus_tag="ACG8M6_001745"
     CDS             10245..11798
                     /locus_tag="ACG8M6_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0457413.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(12179..13963)
                     /locus_tag="ACG8M6_001746"
     CDS             complement(12179..13963)
                     /locus_tag="ACG8M6_001746"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457414.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(13978..15699)
                     /locus_tag="ACG8M6_001747"
     CDS             complement(13978..15699)
                     /locus_tag="ACG8M6_001747"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457415.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            15799..16560
                     /locus_tag="ACG8M6_001748"
     CDS             15799..16560
                     /locus_tag="ACG8M6_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0457416.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         5590 a         3012 c         3282 g         5039 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcaca tcagtcacga tatcaaacaa
     1621 aaaatatacg tctttcttca ttctagtttt cgcagcaggg attttaaagt agttcttatt
     1681 agaatagtag agattaatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatc
     1741 ccacacctta taaaaatctt tgacaaatct attcgaagga tcaaatttaa accataattg
     1801 cgtctcatta aaaagcataa ccattactcc gatctatttt taaacttagt ctctacctct
     1861 aatatatatc gagtttttat tttgccttca gagaatactg tttctttttt tgcagttaca
     1921 ggttgtttat tttcggaaaa agctaatata gctaaaattg aaacatccat ctggaattta
     1981 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
     2041 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
     2101 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
     2161 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
     2221 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
     2281 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
     2341 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
     2401 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
     2461 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
     2521 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
     2581 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
     2641 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
     2701 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
     2761 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
     2821 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
     2881 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
     2941 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     3001 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     3061 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     3121 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     3181 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     3241 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     3301 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     3361 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     3421 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     3481 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     3541 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     3601 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     3661 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     3721 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     3781 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     3841 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     3901 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     3961 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     4021 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     4081 gcacagccaa gaaagactga gtaagtaacc gccgatgata tcagtaggaa aatgaacgcc
     4141 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     4201 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     4261 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     4321 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     4381 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     4441 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     4501 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     4561 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     4621 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     4681 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     4741 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     4801 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     4861 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     4921 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     4981 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     5041 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     5101 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     5161 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     5221 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     5281 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     5341 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     5401 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     5461 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     5521 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     5581 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     5641 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     5701 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     5761 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     5821 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     5881 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     5941 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaattcttc aggaaatgct
     6001 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     6061 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     6121 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     6181 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     6241 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     6301 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     6361 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     6421 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     6481 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     6541 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     6601 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     6661 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     6721 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     6781 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     6841 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     6901 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     6961 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     7021 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     7081 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     7141 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     7201 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     7261 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     7321 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     7381 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     7441 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     7501 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     7561 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     7621 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     7681 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     7741 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     7801 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     7861 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     7921 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     7981 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     8041 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     8101 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     8161 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     8221 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     8281 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     8341 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     8401 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     8461 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     8521 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     8581 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     8641 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     8701 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     8761 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     8821 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     8881 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     8941 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     9001 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     9061 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     9121 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     9181 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     9241 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     9301 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     9361 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     9421 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     9481 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     9541 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     9601 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     9661 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     9721 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     9781 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     9841 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     9901 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     9961 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
    10021 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
    10081 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
    10141 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
    10201 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
    10261 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
    10321 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
    10381 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
    10441 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
    10501 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
    10561 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
    10621 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
    10681 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
    10741 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
    10801 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
    10861 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
    10921 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
    10981 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
    11041 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
    11101 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
    11161 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
    11221 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
    11281 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
    11341 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
    11401 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
    11461 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
    11521 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
    11581 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
    11641 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
    11701 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
    11761 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
    11821 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
    11881 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
    11941 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    12001 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    12061 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    12121 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    12181 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    12241 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    12301 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    12361 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    12421 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    12481 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    12541 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    12601 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    12661 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    12721 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    12781 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    12841 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    12901 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    12961 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    13021 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    13081 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    13141 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    13201 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    13261 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    13321 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    13381 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    13441 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    13501 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    13561 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    13621 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    13681 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    13741 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    13801 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    13861 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    13921 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    13981 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    14041 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    14101 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    14161 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    14221 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    14281 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    14341 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    14401 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    14461 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    14521 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    14581 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    14641 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    14701 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    14761 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    14821 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    14881 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    14941 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    15001 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    15061 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    15121 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    15181 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    15241 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    15301 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    15361 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    15421 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    15481 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    15541 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    15601 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    15661 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    15721 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    15781 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    15841 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    15901 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    15961 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    16021 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    16081 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    16141 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    16201 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    16261 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    16321 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    16381 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    16441 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    16501 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    16561 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    16621 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    16681 tttttagagg aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga
    16741 tgtaattgtc ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc
    16801 atggtgaact gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag
    16861 atgagaatga taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt
    16921 aca
//
LOCUS       DAZKOB010000055        16324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000055 DAZKOB010000000
VERSION     DAZKOB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.055"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG8M6_001749"
     CDS             complement(<1..1835)
                     /locus_tag="ACG8M6_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0457417.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG8M6_001750"
     CDS             complement(1832..2536)
                     /locus_tag="ACG8M6_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0457418.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG8M6_001751"
     CDS             complement(2548..4857)
                     /locus_tag="ACG8M6_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0457419.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG8M6_001752"
     CDS             complement(5052..5516)
                     /locus_tag="ACG8M6_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457420.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG8M6_001753"
     CDS             complement(5516..6142)
                     /locus_tag="ACG8M6_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0457421.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG8M6_001754"
     CDS             complement(6149..6514)
                     /locus_tag="ACG8M6_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0457422.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG8M6_001755"
     CDS             complement(6504..6842)
                     /locus_tag="ACG8M6_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457423.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG8M6_001756"
     CDS             complement(6832..7158)
                     /locus_tag="ACG8M6_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0457424.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7136..7417)
                     /locus_tag="ACG8M6_001757"
     CDS             complement(7136..7417)
                     /locus_tag="ACG8M6_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0457425.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(7419..8783)
                     /locus_tag="ACG8M6_001758"
     CDS             complement(7419..8783)
                     /locus_tag="ACG8M6_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0457426.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG8M6_001759"
     CDS             complement(8796..9365)
                     /locus_tag="ACG8M6_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0457427.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG8M6_001760"
     CDS             complement(9337..10524)
                     /locus_tag="ACG8M6_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0457428.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG8M6_001761"
     CDS             complement(10588..12315)
                     /locus_tag="ACG8M6_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0457429.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG8M6_001762"
     CDS             complement(12312..12764)
                     /locus_tag="ACG8M6_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0457430.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG8M6_001763"
     CDS             complement(12876..13256)
                     /locus_tag="ACG8M6_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0457431.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG8M6_001764"
     CDS             complement(13253..13639)
                     /locus_tag="ACG8M6_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0457432.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG8M6_001765"
     CDS             complement(13641..13919)
                     /locus_tag="ACG8M6_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457433.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG8M6_001766"
     CDS             13967..14695
                     /locus_tag="ACG8M6_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0457434.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14841..15317)
                     /locus_tag="ACG8M6_001767"
     CDS             complement(14841..15317)
                     /locus_tag="ACG8M6_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0457435.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15277..15633)
                     /locus_tag="ACG8M6_001768"
     CDS             complement(15277..15633)
                     /locus_tag="ACG8M6_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457436.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15611..15796)
                     /locus_tag="ACG8M6_001769"
     CDS             complement(15611..15796)
                     /locus_tag="ACG8M6_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457437.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15797..16069)
                     /locus_tag="ACG8M6_001770"
     CDS             complement(15797..16069)
                     /locus_tag="ACG8M6_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0457438.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16066..16266)
                     /locus_tag="ACG8M6_001771"
     CDS             complement(16066..16266)
                     /locus_tag="ACG8M6_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457439.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT         4571 a         3455 c         2539 g         5759 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 ggtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta catagctaca atgaccgaac
    14821 atttagtgaa cagtgtaatt ttagaataaa gagagttgtt ctttgtactt ctcgctattg
    14881 taaaaatcaa tttcgttttc ttttttcaat cggtcagctt gtctgtgttc atactcatct
    14941 aaaaagttca atgtctttct aatttctgca tgtcgctgtc tgacatacga tgagctatac
    15001 ccaacagatt cagcaatatc ttctaagctc atttgatcaa catatttcat cttaacaatt
    15061 tgattatcga taccagaaaa gctatctatc agcaataaca tttcttcttt ttgctcgatc
    15121 aacaattcca attcattttc tatcttttgg atattctctt ctaaagaaga agttctcgaa
    15181 ttcttttcga tgcggacgtt tgctaaatca ccacagaccc atcgattcaa ttcaagctta
    15241 cttttattaa gattccactt taagtaaaga atctgctcat ctagttcttg gtaatctttt
    15301 aaccaatgaa atctcacaaa cgccacccct tatggtagaa tagtattgcg ctgctatctc
    15361 gaaaggggta gcttttttat tttttcttag ccgttccatt ctggattgcc ttttgttcca
    15421 aacgtcgttt tttcttctta atctttgatt ttgtttttcc catacaattc tcctttccga
    15481 aaacacattt tgttcgattt ttaatactaa ataaaagttg gccagaattt ttcgttcgtc
    15541 tttactcatc tttttttgaa taagattact agcataaaaa tagttagtac cacgaaaatg
    15601 aacaggtaaa tcaattacgt tcaccttctt catgccagtt atcagccact agaatctttg
    15661 cgttagtgcc gccaccgttt tcttttttgt atttttcaaa tgctttataa gcagctgttt
    15721 ttgatttata gatttttccg gaaccgactg ggaacatcca tgtgccctct gtgatagcta
    15781 agatgtatac tttcatttaa ttcacccctt taatccccaa cactacatat ccggacttct
    15841 gcatgtaatc agtaatataa gtgatctctg ctttgtatga gtcaccagtg taactaaatg
    15901 ttggtctcgt tagtccaggt aattcatgtt taatgtattt tttatattca cgtagtatta
    15961 actgatcacc aactttgaag tcacgatcat tcttgcggat ctcgaattgt ttgcgtccgc
    16021 tcgtcactgc ttcaaaatat tctggtagta tttttagttc gtggatcatt ctgtttcctc
    16081 cttcaaaatt cgctcatcat caaaaagtga atctccacaa aatccttctc ccaaatattt
    16141 atcgttcatg tataagtcaa ccaaatattt gtgatctgct tctcgatatt cattaatatt
    16201 aacgatcttt attttgtaaa cattaccttc gtcgctcact atcttgaatt gttctctgac
    16261 tttcaccacc gttcctcctc tttttgaaaa aattttagtg tttttgtaag accccctaaa
    16321 tcag
//
LOCUS       DAZKOB010000056        16194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000056 DAZKOB010000000
VERSION     DAZKOB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.056"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..451
                     /gene="scm"
                     /locus_tag="ACG8M6_001772"
     CDS             <1..451
                     /gene="scm"
                     /locus_tag="ACG8M6_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0457440.1"
                     /translation="STDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTS
                     ASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKT
                     SNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(606..1592)
                     /locus_tag="ACG8M6_001773"
     CDS             complement(606..1592)
                     /locus_tag="ACG8M6_001773"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0457441.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1615..2181)
                     /locus_tag="ACG8M6_001774"
     CDS             complement(1615..2181)
                     /locus_tag="ACG8M6_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0457442.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2195..2611)
                     /locus_tag="ACG8M6_001775"
     CDS             complement(2195..2611)
                     /locus_tag="ACG8M6_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0457443.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3068..5002)
                     /locus_tag="ACG8M6_001776"
     CDS             complement(3068..5002)
                     /locus_tag="ACG8M6_001776"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0457444.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5304..6239
                     /gene="pplA"
                     /locus_tag="ACG8M6_001777"
     CDS             5304..6239
                     /gene="pplA"
                     /locus_tag="ACG8M6_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0457445.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6329..7432
                     /locus_tag="ACG8M6_001778"
     CDS             6329..7432
                     /locus_tag="ACG8M6_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0457446.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7445..8398
                     /gene="menA"
                     /locus_tag="ACG8M6_001779"
     CDS             7445..8398
                     /gene="menA"
                     /locus_tag="ACG8M6_001779"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0457447.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9212..10558)
                     /gene="gor"
                     /locus_tag="ACG8M6_001780"
     CDS             complement(9212..10558)
                     /gene="gor"
                     /locus_tag="ACG8M6_001780"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0457448.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10692..11945)
                     /locus_tag="ACG8M6_001781"
     CDS             complement(10692..11945)
                     /locus_tag="ACG8M6_001781"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0457449.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11951..12874)
                     /locus_tag="ACG8M6_001782"
     CDS             complement(11951..12874)
                     /locus_tag="ACG8M6_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0457450.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13095..15587)
                     /locus_tag="ACG8M6_001783"
     CDS             complement(13095..15587)
                     /locus_tag="ACG8M6_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0457451.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15594..16061)
                     /locus_tag="ACG8M6_001784"
     CDS             complement(15594..16061)
                     /locus_tag="ACG8M6_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0457452.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4757 a         3386 c         2880 g         5171 t
ORIGIN      
        1 cagtacggat acatctacca caacaagtga tagcacggac acgtcagctt caagtgacag
       61 tacagatact acctctacca caagtgatag ctcagattca tctaccacta caagtgatag
      121 cacggacacg tcagcttcaa gtgacagtac agatacatcc tctaccacaa gtgatagctc
      181 agattcatca acttcaagcg atagtacaga cacgtcgtct accacaagcg atacacgtca
      241 tgcaagtgtc tccttctcaa aaacaagttc ggataacagg acgaacttta acttaactaa
      301 taaaacaagc aatgacaatg gaaacaaatc aaaaagagca cttcctaaaa ctggaagtca
      361 atcgaataat tggataacac tcgcaggcgt tatattactt gtaatcggtt taagaatcac
      421 ttttatgagt tataagaaat cgaaaaggta aaaaaatatt caagataaaa aagagcaacg
      481 tcgctaacgg acgttgctct ttatttttgc tttttatcat aaatacaaga actacaattc
      541 aatttgtcaa aaaacacgta gatttccttc tgagaaattt atttacgtat cgaacctttc
      601 cctattcagt ctttgcgatc caaaatcagt tgggtaaggg aaaagagatt ctcttttgtt
      661 cgaagagagg tggcaggtaa tttctttatt tccttcaaag ccttttctgt gtaagaggcc
      721 gctaacttcc tagcttcttc cacgcctcct gtatgttcaa cgattttgcg tacggcgtcg
      781 acatcttctt ctgtcattgc cgctttcttc tctaaataag gtagtagctt tggtttgttt
      841 ttttgcaaag agtagatcaa cggtaaagaa tagacccctt gacgcatgtc ttctagtaca
      901 ggtttgccga tttcctggct ctcttttgta taatctaaaa tatcatcgat aatctgaaag
      961 gccatcccga tagaaagtcc gatatctcct gctttttttg cgaagcgctc gcttgttcca
     1021 ctttcaaaag cgccaacaga acaacttaat tggaacagtt cagctgtctt acctgaaata
     1081 ttttccaaat attcttttac agaaagttca taattatagc gatcatccat ctgccctaat
     1141 tcgccattca atactttttc catactgcga gaattcaatt ggagactttt caaggaagtg
     1201 gagtaatcag acaatagttt aaaacagcag acaaataaat agtctcctgc atagacagaa
     1261 acgacattac caaactgact acggatatta ggctgaccac gtcgcagatc cgcttcatca
     1321 acgatatcat catggatcag tgtggccgta tggagcattt cgatagaagc tgccaaagcg
     1381 atggctttgt ctcgatcttg ctctggtcca aattgtgaga acaatagctg ataggcagga
     1441 cgcaacagct ttccgccggc atggatcgtt tctttgattg cttgttcgac aggtttgttt
     1501 ttcaggcgga cagtttcttc catccgtttt agtgtttctg tcaattcttt acttaaagca
     1561 ggatagtttt cccacattgg atgtagcttc ataaaaaaac tccttgtgtg acgattattt
     1621 tttggtaaac attgggctcc atttttgttt ggttccctgc tcataatctt tttggaatcg
     1681 ttgaagtgtc tgcacatgtc gtaaaagata attcgctgcg atcccgattg caatccctga
     1741 taaaatccct aaaactgaaa ggactggcaa gtacaacatg actgtccatg attgtgcaat
     1801 gaaagaagcc atcaaaagct gtccgacatt atgcatgaaa ccgccggccg cactaattcc
     1861 gatgatactg acacgttttg gtcctaattg cttgaccaat aacattccaa aataactaag
     1921 caaagcacca ctcatactat aaaggaatgt ggaaatcgtt ccgcctagca aagtagtcag
     1981 gaatagtcgt agccaaagta gtaagaagct gtctcgtttt ttcatggtaa aaatggcaac
     2041 gatcgtgatt agattggcta agcctagttt tgcaccaggc gcaaaagaaa aaggataagg
     2101 gatcatattt tccaataaac caatgatcac tccttgtgca acaagtagcg aaatataaat
     2161 cattttttgt aatttactca tggtgtgtga aggtctaata aatcagactg tcttcgtcac
     2221 ctccatctgt tgaacggact tcgatcacta atttatgtgg caaacagacg atggtttcgc
     2281 catttttcgt tgcccagcct cgacgtacgc agatctggtc atcgcaatct gcttctttta
     2341 tccgtactcg atcgccatct atctcgatca agttataatc tccgtgttca tctttgtaag
     2401 tatatgtgtt gctctcttct ccagccatca aagggaatgt tttgatttct gtgccatcca
     2461 cacgcagtac cgcttctttt tctgctgttg cggtgttttg ctgaagacca aaaatcatga
     2521 ctggtaaaaa agaaagaaca gtcaaaagca aaataataaa aacatcccaa ggtttgagca
     2581 gacttttttc agcaaattct ttaaggctca tttttgttct cctttttcca gattaactca
     2641 cccaatttta gcataaaaac aagaggttgt gacacctgtg tcttgctttg agtaataaga
     2701 acgaagaata gaaaacagct ttccatgttt tctaattctt tgggcttatt accgagaagc
     2761 agcaggtgga acaccgttta taacaagagg ttgtgacaga agtgggcaac ttcaagaaat
     2821 aagaaggaat ttacgaaaat tacttttcaa gtttttgtga aatttcggct tatttttcga
     2881 agaagctact tctggaacac cgtttataac aagaggttgt aacagaggtt gtgacacctg
     2941 tgtcttgctc atggaggctt atctcgggca tcttattcct ttaattttac ataaaaagga
     3001 ggctgtgacg gtttgtctca gcccctttct attgaaatct tgcattcaaa taacgttctt
     3061 tatctattta tttcttgccg tactgggaac ttggtctacc gtaccaccaa ttacctagtg
     3121 tacgttgtag ccatggaatt acccagcgat ccaacccaat cgcacgtccg gaaccgttca
     3181 tcaatgcgaa agcaacaaaa atgaaccaaa tgtttaccca gtaaaacatg cctgataagc
     3241 agaacgtgat caccaataca atcgttgtcg cactgcatag ccatgtgaat aatccagcaa
     3301 tcaatgctaa tgccaaggca atttcaaaga ataccatgaa tttttggaag aacattgcta
     3361 catcattgtt tggcatgatc acttgcatga tctttgtgaa ccagtctggt gacttctcaa
     3421 tcacagccat tggttcttca ccgtatgcat aactcaaacc aaaatgtgca gcttgagtcg
     3481 ctgcttctcc ggcacttgcc cctgctccgc tggcagctgt ggttgcatca ccagcagcag
     3541 aagcaccact ggcaacatct gttacattgt attcccatgt ccaagggaac acactgtttc
     3601 cagtgaacca tgatccttca ccaaaccaag tacttggttt gaacacttca ccattcccaa
     3661 tgattttttt ggaagcttca acgaaccaaa ctaaaccata gaaaatacga agtggcacac
     3721 tccataagac attgccgtaa cgagaagaat gtcctcgagt cacgttgcga tcatctttga
     3781 tatggaaaaa ctcatgcatt aaatattgga acatataata accagaacga atatcaaaga
     3841 agtatttcaa attcacgata tgtttcatta cgatagctaa aaatccgctt aaatggatct
     3901 tatcaaatag gttagctacc ccccatttgg caccaataga aaccataaat ccttgatagt
     3961 ttcctttaaa tttatgcttt tctccgcctt tgatcgaagc cacgatattc gctgcggctg
     4021 tgtgtcctgt ttgttcagca gcttgaacga tctgcggtgt cggtgttcct gggaattctt
     4081 cataaaagac taaatcacca atgatataga tgtttttatc ttcgtagcct tttgcctgca
     4141 tatattcatt ggcgatcaac cgctgtccgc gtgctgattc taatccaaag tcagcagcgt
     4201 cagaagttcc tttgacacca gctgtccaaa tcagtgtgtg agtcggaatg ttgcgtccgt
     4261 ctttcaattt gatatgatca gcagccactt ctacgattgg tgcattcaat aataattcca
     4321 cgttgtgctt acgcaaatag cgttcagctt ttgcagcatc atttcgatcc aacatattca
     4381 agatcgtcgg cattgcctct acgaccatca gtgtgatttc gtttggatct aatttataat
     4441 ctttggctag gcgatttttc cagtcgatca gttccccaat catttcaata ccggtaaatc
     4501 cagaaccaca gacaacgaaa gtcagcatgg ctttacgcag ttcatcatct ggttccaatg
     4561 cagcttttgc tactgtcttc tcaatatgtt cccgaatcct tacagaatct tcaaatgacc
     4621 aaagggtgaa tccgtgtgct ttgacaccag gtgtaccaaa gtcattcggc tctccgccca
     4681 ttccaaggat cagctgatca aaaggatagg aacctaattt ggtcttgacg atttttcgtt
     4741 ctttatcgat accgattaca gtgtctgtga caagttgtac attcttttga cgagcaaaca
     4801 aatgctgcag atcgtattgg attgcttctg gttctacacg tccgccagct acttcatgca
     4861 actcggtcat catagtatgg tacgaatgac gatcgatcag agtaattgct acatcttctt
     4921 ttttcagttt ctttgccaag tattttgtag ctgaaacacc ggcatatcct gctcctacaa
     4981 cgacaatatt ttgctttgcc attgatcatc cgctccttat agactatcta tataatgaat
     5041 atatgataat tcttttagtt tgatacttat tacacaatct ccatatatta tatagatgtt
     5101 ctgttctttt gtctaactcc ttttggattt ttttcaaatt ttaaaataaa gcgttttttc
     5161 acgaaatttg ctgaaagaaa acgtttgaaa tattacatgg gttatttatc aaaaatagtt
     5221 gacttatagg ctaaaaagat tagaatgtat aatgaatcac aaagaaaaat tgtggactag
     5281 aaatggaatg gtggggtatt aaaatgaaga aaaaacgttt tgtaactgga tttgccgtat
     5341 tagcattttc agctctagtg ttaggcgctt gcggcgcaga tgacaacgga agctcaaact
     5401 cttcttctga atcatctaca gcacaatctt cgactgcaaa aagcacaaca gaatcatctg
     5461 caaaagttgt agctggcgga gatttgcaag acggcacata taaattagaa gaaaaaaatt
     5521 attcaaatgg ttaccgtgca gtctttgaaa tggttgtaaa agacggcaaa atcactgaat
     5581 caaaatatga taacgtcaat gaaaacggcg aatcaaaaac aaaagatgca gaatacaaca
     5641 aaaacatgga agcaaaatct ggcacaaacc cagaaaaata cattcctgag ttgaatgacc
     5701 aattcttaaa agcacaaagc gcaagcggtg tggaagtcgt aactggagct actcactcat
     5761 ctgaaagctt ccaaaactac gcacaacaat tgatccaagc tgctcaagct ggtaacacag
     5821 atacaatcga aatcgacaat ggtgctgatt tgaaagacgg cacttacaag cttgaagaaa
     5881 agaactactc taacggctac cgtgtgcaat tcgaaatgac agttgctggc ggcaaagtaa
     5941 ctgaatcaaa ctttgattat atcgacaaag acggtaaatc aaaacaagat gatacagaat
     6001 ataatgaaaa tatgaaagca aaaagcggta cagaaccaaa aacttacatc cctactctga
     6061 acgacgaatt cgtcaaagca atgggcgaag aagatggctc acctgctaat gtagaagttg
     6121 ttacaggtgc tacacacagc tcacattcat tcatcatgta cgcacaacaa ttagtgaatg
     6181 cagctgaaaa aggcgacact caaacaatcg aagtagataa tatcgtaaca gaaaaataat
     6241 cacataatca tactaataaa agttggaggg tgggacgata gccagattag gcagtcgtct
     6301 cacctttcaa aatgaagaga ggaaaaagat gcggaaaaaa ctactatttt ccttaatcac
     6361 actgggcatc tcttttatgg tgctaactgg ctgcagcaac tcagcaaata aaacaaccac
     6421 tgaaagttca gcagctacca agatattgaa agatccatat tctgatgaac agttcttgtt
     6481 agggacttac gttcgtatcc gtgtgtatga tgaaggcaaa gaatcagcat tgaaacctgc
     6541 cttcgatcga gtaaaagaac ttggtgataa aatcacgatc aatcaaaaag gttctgagat
     6601 cgatgaagtg aatgagcaag ctgggatcaa accagtaaaa gtttctgatg acgtctacac
     6661 gttagtcaag cgggcctacg aatatagcca agattctcaa ggcggattcg atatggcgat
     6721 tggtgcaatc acacaattat ggcgaatcgg ttttgatgac gccagaaaac cttcccaaga
     6781 agaaatcgat caggcattga aattagtgga ttatcataag atcgaactaa atgacaaaga
     6841 aaaaactgtt tatctaaaag aaaaaggaat gatcatcgat ttaggtgcaa tcgccaaagg
     6901 ttacattacg gacgaagtag tgaaagtctt gaaaaaacaa ggagtgacca ctgccatcgt
     6961 tgacttgggc ggtaatgtct atgtcttagg ccacagccca agaggcgaaa accaagactg
     7021 gacagtcggc atccaagatc cgaacatggc tcgcggatct gttttaggtt ctattaaaga
     7081 acggaacaaa acattagtta cctctggaat ttacgagcgt tatttagaag tagatggcaa
     7141 aaaataccat catttgtttg atccaaaaac gggttatccg ttcgacaatg atatcgcaag
     7201 tgtcacgatc ataacagata aatcaattga tggtgacggg ttgtctacag cagtcttttc
     7261 aatgggtgtg aaaaaaggac tggaatacgt cgaaagcgaa ttgaataacg ggacggaagc
     7321 gatcttcgtg acaaaagacg ataaagtata tgtgaccgat ccaatcaaag acaccttcaa
     7381 attatcggag gactcccatt acacgatggg tgaccgttct gaactaaaat aaggagtacg
     7441 aaccatgaca ctaaaaatct ttttagaagt agttgaaata cgaacaaagg tggctagtgt
     7501 atttcctttt gcggtagggg tattattctc tatcgctttt tttcatgaga tacattggct
     7561 aaacactttt ttatttttta ttggaatgct agtttttgat ttagcgacta ccgctatcaa
     7621 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
     7681 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
     7741 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
     7801 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
     7861 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
     7921 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
     7981 atttgctttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
     8041 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
     8101 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
     8161 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
     8221 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
     8281 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
     8341 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
     8401 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
     8461 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
     8521 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
     8581 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     8641 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     8701 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     8761 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     8821 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     8881 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     8941 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     9001 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     9061 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     9121 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     9181 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     9241 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     9301 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     9361 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     9421 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     9481 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     9541 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     9601 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     9661 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     9721 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     9781 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     9841 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     9901 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     9961 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
    10021 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
    10081 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
    10141 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
    10201 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
    10261 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
    10321 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
    10381 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
    10441 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
    10501 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
    10561 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
    10621 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
    10681 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
    10741 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
    10801 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
    10861 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
    10921 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
    10981 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
    11041 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
    11101 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
    11161 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
    11221 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
    11281 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
    11341 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
    11401 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
    11461 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
    11521 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
    11581 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
    11641 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
    11701 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
    11761 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
    11821 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
    11881 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
    11941 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
    12001 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
    12061 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
    12121 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
    12181 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
    12241 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
    12301 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
    12361 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
    12421 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
    12481 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
    12541 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
    12601 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
    12661 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
    12721 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
    12781 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
    12841 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
    12901 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
    12961 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
    13021 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
    13081 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
    13141 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
    13201 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
    13261 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
    13321 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
    13381 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
    13441 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
    13501 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
    13561 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
    13621 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
    13681 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
    13741 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
    13801 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
    13861 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
    13921 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
    13981 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
    14041 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
    14101 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
    14161 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
    14221 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
    14281 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
    14341 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
    14401 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
    14461 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
    14521 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
    14581 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
    14641 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
    14701 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
    14761 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
    14821 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
    14881 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
    14941 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
    15001 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
    15061 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
    15121 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
    15181 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
    15241 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
    15301 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
    15361 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
    15421 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
    15481 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
    15541 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
    15601 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
    15661 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
    15721 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
    15781 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
    15841 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
    15901 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
    15961 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
    16021 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
    16081 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
    16141 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DAZKOB010000057        16155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000057 DAZKOB010000000
VERSION     DAZKOB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.057"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG8M6_001785"
     CDS             226..894
                     /locus_tag="ACG8M6_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0457453.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG8M6_001786"
     CDS             complement(1094..1414)
                     /locus_tag="ACG8M6_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457454.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG8M6_001787"
     CDS             complement(1451..2833)
                     /locus_tag="ACG8M6_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0457455.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG8M6_001788"
     CDS             complement(2989..4176)
                     /locus_tag="ACG8M6_001788"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0457456.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG8M6_001789"
     CDS             complement(4183..5214)
                     /locus_tag="ACG8M6_001789"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0457457.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACG8M6_001790"
     CDS             complement(5230..6684)
                     /locus_tag="ACG8M6_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0457458.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="ACG8M6_001791"
     CDS             6859..7764
                     /locus_tag="ACG8M6_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0457459.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG8M6_001792"
     CDS             7855..8532
                     /locus_tag="ACG8M6_001792"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0457460.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG8M6_001793"
     CDS             8529..8699
                     /locus_tag="ACG8M6_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457461.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG8M6_001794"
     CDS             8813..9709
                     /locus_tag="ACG8M6_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0457462.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG8M6_001795"
     CDS             complement(9764..11134)
                     /locus_tag="ACG8M6_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0457463.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG8M6_001796"
     CDS             11372..12118
                     /locus_tag="ACG8M6_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457464.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG8M6_001797"
     CDS             12115..12954
                     /locus_tag="ACG8M6_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0457465.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACG8M6_001798"
     CDS             12955..13905
                     /locus_tag="ACG8M6_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0457466.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACG8M6_001799"
     CDS             14046..14798
                     /locus_tag="ACG8M6_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457467.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACG8M6_001800"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACG8M6_001800"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0457468.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5242 a         3011 c         3243 g         4659 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatggatt attgcagtga atgttacaga atctgcgttt ttcattcaaa
    16141 aataaaaaaa catag
//
LOCUS       DAZKOB010000058        15949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000058 DAZKOB010000000
VERSION     DAZKOB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.058"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..550
                     /locus_tag="ACG8M6_001801"
     CDS             <1..550
                     /locus_tag="ACG8M6_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457469.1"
                     /translation="VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQ
                     FFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAK
                     KRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLK
                     AEIIKIRNEKNRSILEKLDFVN"
     gene            complement(576..1016)
                     /locus_tag="ACG8M6_001802"
     CDS             complement(576..1016)
                     /locus_tag="ACG8M6_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0457470.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1176..1682
                     /locus_tag="ACG8M6_001803"
     CDS             1176..1682
                     /locus_tag="ACG8M6_001803"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0457471.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1850..3763
                     /locus_tag="ACG8M6_001804"
     CDS             1850..3763
                     /locus_tag="ACG8M6_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0457472.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3768..4769
                     /locus_tag="ACG8M6_001805"
     CDS             3768..4769
                     /locus_tag="ACG8M6_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0457473.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4971..5177)
                     /locus_tag="ACG8M6_001806"
     CDS             complement(4971..5177)
                     /locus_tag="ACG8M6_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0457474.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5823..6671)
                     /locus_tag="ACG8M6_001807"
     CDS             complement(5823..6671)
                     /locus_tag="ACG8M6_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0457475.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            6835..7737
                     /gene="gnd"
                     /locus_tag="ACG8M6_001808"
     CDS             6835..7737
                     /gene="gnd"
                     /locus_tag="ACG8M6_001808"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0457476.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            7739..8740
                     /locus_tag="ACG8M6_001809"
     CDS             7739..8740
                     /locus_tag="ACG8M6_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0457477.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            8737..9021
                     /locus_tag="ACG8M6_001810"
     CDS             8737..9021
                     /locus_tag="ACG8M6_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0457478.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            9054..9851
                     /locus_tag="ACG8M6_001811"
     CDS             9054..9851
                     /locus_tag="ACG8M6_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0457479.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            9865..10698
                     /locus_tag="ACG8M6_001812"
     CDS             9865..10698
                     /locus_tag="ACG8M6_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0457480.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            10716..11573
                     /locus_tag="ACG8M6_001813"
     CDS             10716..11573
                     /locus_tag="ACG8M6_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0457481.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            11595..12104
                     /locus_tag="ACG8M6_001814"
     CDS             11595..12104
                     /locus_tag="ACG8M6_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0457482.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            12141..12542
                     /locus_tag="ACG8M6_001815"
     CDS             12141..12542
                     /locus_tag="ACG8M6_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457483.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            12654..13007
                     /locus_tag="ACG8M6_001816"
     CDS             12654..13007
                     /locus_tag="ACG8M6_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457484.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            13041..13484
                     /locus_tag="ACG8M6_001817"
     CDS             13041..13484
                     /locus_tag="ACG8M6_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0457485.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            13501..14586
                     /gene="uxuA"
                     /locus_tag="ACG8M6_001818"
     CDS             13501..14586
                     /gene="uxuA"
                     /locus_tag="ACG8M6_001818"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0457486.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            14579..15292
                     /locus_tag="ACG8M6_001819"
     CDS             14579..15292
                     /locus_tag="ACG8M6_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0457487.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(15499..15648)
                     /locus_tag="ACG8M6_001820"
     CDS             complement(15499..15648)
                     /locus_tag="ACG8M6_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457488.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         5533 a         2487 c         3365 g         4564 t
ORIGIN      
        1 tgtcaatttg gcaatgatcc atttgtattc aagggttttt gggacaaaaa agaaggtgga
       61 tgcttttgga aaatcagcag aagacagaga ttttcagcat atatttccag tgatgtatcc
      121 aatcatgcaa cagtttttta gtggggtagc tcgcagaatt ccagagctta atacttatta
      181 tgaaaagaac agacggttga tttttcaata ctgtatgtta gtcagggata tttttataaa
      241 acacgaacaa cctgtcatat tctatattca atcaaagtat ggtaaaacac aggaattatg
      301 ggcaaagaaa aggatattat ctatgactga acgcccaata aaatatagtg catccgctga
      361 tcagttacct gatattgtca ttagcgatta tcctatcgac aaaaaggcat atgatcaaac
      421 gaatacacag atattttact ggaatggaca acctacaaaa aatgattggg cgcgtctgaa
      481 agctgaaatc ataaaaatca ggaatgagaa aaatagatcc atattggaaa aattggattt
      541 cgttaattaa gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt
      601 tctttaaaat cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta
      661 gttttagaaa ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat
      721 tggtcgtaga taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt
      781 tcactcaaac tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta
      841 gagagcattt cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg
      901 gttagtggtt gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa
      961 cgttttgcgc gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat
     1021 tcctccaata gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat
     1081 ttagaggtgt aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata
     1141 ctattaaatc aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag
     1201 tagtgatcga tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa
     1261 cagtgacagc attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta
     1321 ttttcgttca acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt
     1381 cggaattatc tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt
     1441 ataagactaa tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg
     1501 gggcacaagc agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc
     1561 agcttatgat gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg
     1621 aaacaatcgc tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat
     1681 aagttgtttc ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt
     1741 acgtaaaatg agaaaataat cctgttctat ttttatggga taaaccattt attttttgta
     1801 taaaaaatgt ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt
     1861 gagaacaaaa caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt
     1921 cccgcaatgg acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga
     1981 aaccacagaa actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac
     2041 aaatactgaa acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac
     2101 cacagataac caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc
     2161 ttctgctaaa gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga
     2221 aaatttcaat ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa
     2281 tggaccaaca gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt
     2341 taatgattat ggtgtagata aagagattat caaacatctg attgctactt atctattgta
     2401 cgatatcgct tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg
     2461 gacaacgcct acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt
     2521 actaaacaaa caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc
     2581 agataaaaca gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac
     2641 agaagaatac aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc
     2701 gactggtaat agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct
     2761 ttactggata aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg
     2821 ttcgatggga gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa
     2881 aggaattatt gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag
     2941 ccagacagta gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga
     3001 tcaagagcat gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat
     3061 gcatttggat acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt
     3121 gattatccat ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga
     3181 gttatcagga gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca
     3241 ggaagttgac catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca
     3301 tgatcagata aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga
     3361 cgaaatccag ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga
     3421 aggatacgaa ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc
     3481 aactattatt ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac
     3541 agagaatgca gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac
     3601 gcaaccgatt cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt
     3661 gccgaaaact ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt
     3721 tagcacgatt atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca
     3781 agcgaatagt gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga
     3841 ttaatcaaat cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg
     3901 aaaaagaaca agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata
     3961 taaaaaaaga aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac
     4021 cgaaagtgga aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag
     4081 gaggggataa aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa
     4141 tcatcgtaaa tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc
     4201 aattgaacga agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga
     4261 atatttcgat tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac
     4321 ttcaaaatga agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga
     4381 ttacgaagtt tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg
     4441 ataaaatctc tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact
     4501 ttgaacaatc agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc
     4561 aaaacacatt cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa
     4621 cttctgtatt tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg
     4681 ctgttttgaa ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc
     4741 aaaagttagt aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg
     4801 tcacttagct ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa
     4861 tattgtaact tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg
     4921 ggaatatcac acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt
     4981 tgttttgatc gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa
     5041 aagccgagtg gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa
     5101 ggctgggaac aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa
     5161 atatggatag ctttcataag cagcacctcc ttttttataa ttgtatgata aatagattaa
     5221 gaatctataa atgtgacaag tttgttagga tgtgaataaa atgatcatct agcgaacttg
     5281 aaacaaagat ggctaatgat aagcaattaa tgattcagca atcagatagg tacaaagttt
     5341 accatcatac gtctagacca ataatagaaa atatgggaat ataatagaaa agatttagga
     5401 tacccccaaa agaatttccc caaaaattcg atgtatccta aactttttta ttttacataa
     5461 caatcatatc aactgtgaaa taaaattaca aatgataacc taaaatgagg ccaaaagcca
     5521 gtgttatcaa ggaaatactt aattcaccaa aatttttttt aattttttag aaaataagaa
     5581 acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat
     5641 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
     5701 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
     5761 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
     5821 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
     5881 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
     5941 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
     6001 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
     6061 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
     6121 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
     6181 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
     6241 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
     6301 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
     6361 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
     6421 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
     6481 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
     6541 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
     6601 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     6661 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     6721 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     6781 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     6841 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     6901 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     6961 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     7021 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     7081 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     7141 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     7201 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     7261 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     7321 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     7381 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     7441 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     7501 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     7561 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     7621 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     7681 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     7741 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     7801 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     7861 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     7921 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     7981 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     8041 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     8101 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     8161 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     8221 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     8281 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     8341 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     8401 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     8461 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     8521 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     8581 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     8641 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     8701 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     8761 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     8821 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     8881 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     8941 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     9001 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     9061 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     9121 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     9181 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     9241 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     9301 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     9361 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     9421 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     9481 tagttggagg attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg
     9541 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     9601 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     9661 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     9721 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     9781 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     9841 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     9901 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     9961 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
    10021 gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga
    10081 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
    10141 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
    10201 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
    10261 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
    10321 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
    10381 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
    10441 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
    10501 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
    10561 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
    10621 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
    10681 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
    10741 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
    10801 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
    10861 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
    10921 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
    10981 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
    11041 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
    11101 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
    11161 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
    11221 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
    11281 agcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
    11341 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
    11401 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
    11461 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
    11521 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
    11581 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
    11641 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
    11701 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
    11761 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
    11821 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
    11881 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
    11941 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
    12001 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
    12061 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
    12121 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
    12181 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
    12241 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
    12301 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
    12361 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
    12421 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
    12481 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
    12541 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
    12601 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
    12661 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
    12721 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
    12781 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
    12841 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
    12901 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
    12961 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
    13021 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
    13081 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
    13141 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
    13201 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
    13261 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
    13321 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
    13381 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
    13441 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
    13501 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
    13561 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
    13621 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
    13681 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
    13741 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
    13801 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
    13861 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
    13921 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
    13981 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
    14041 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
    14101 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
    14161 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
    14221 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
    14281 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
    14341 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
    14401 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
    14461 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
    14521 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
    14581 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
    14641 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
    14701 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
    14761 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
    14821 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
    14881 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
    14941 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
    15001 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
    15061 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
    15121 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
    15181 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
    15241 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
    15301 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
    15361 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
    15421 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
    15481 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
    15541 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
    15601 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    15661 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    15721 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    15781 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    15841 ttcgaagaca atagaagaca atgaaattta tatttcattg cattatcctt gtgttattag
    15901 aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttga
//
LOCUS       DAZKOB010000059        15742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000059 DAZKOB010000000
VERSION     DAZKOB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.059"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..722)
                     /locus_tag="ACG8M6_001821"
     CDS             complement(24..722)
                     /locus_tag="ACG8M6_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0457489.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ACG8M6_001822"
     CDS             complement(715..2067)
                     /locus_tag="ACG8M6_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0457490.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ACG8M6_001823"
     CDS             complement(2290..3690)
                     /locus_tag="ACG8M6_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0457491.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ACG8M6_001824"
     CDS             3851..4588
                     /locus_tag="ACG8M6_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0457492.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ACG8M6_001825"
     CDS             4713..5192
                     /locus_tag="ACG8M6_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0457493.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ACG8M6_001826"
     CDS             5189..5956
                     /locus_tag="ACG8M6_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0457494.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8M6_001827"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ACG8M6_001827"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0457495.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ACG8M6_001828"
     CDS             complement(6859..7461)
                     /locus_tag="ACG8M6_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0457496.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..7797
                     /locus_tag="ACG8M6_001829"
     CDS             7567..7797
                     /locus_tag="ACG8M6_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457497.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7788..8036
                     /locus_tag="ACG8M6_001830"
     CDS             7788..8036
                     /locus_tag="ACG8M6_001830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0457498.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8142..8726
                     /locus_tag="ACG8M6_001831"
     CDS             8142..8726
                     /locus_tag="ACG8M6_001831"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0457499.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8M6_001832"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ACG8M6_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0457500.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8M6_001833"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ACG8M6_001833"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0457501.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ACG8M6_001834"
     CDS             10627..10806
                     /locus_tag="ACG8M6_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457502.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ACG8M6_001835"
     CDS             10828..11853
                     /locus_tag="ACG8M6_001835"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0457503.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8M6_001836"
     CDS             12028..13272
                     /gene="glyA"
                     /locus_tag="ACG8M6_001836"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0457504.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ACG8M6_001837"
     CDS             13425..14372
                     /locus_tag="ACG8M6_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0457505.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ACG8M6_001838"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ACG8M6_001838"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0457506.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ACG8M6_001839"
     CDS             complement(15274..15516)
                     /locus_tag="ACG8M6_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457507.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ACG8M6_001840"
     tRNA            15637..15709
                     /locus_tag="ACG8M6_001840"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5349 a         2823 c         3195 g         4375 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt tt
//
LOCUS       DAZKOB010000060        15585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000060 DAZKOB010000000
VERSION     DAZKOB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.060"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..222
                     /locus_tag="ACG8M6_001841"
     CDS             55..222
                     /locus_tag="ACG8M6_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0457508.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(284..2095)
                     /locus_tag="ACG8M6_001842"
     CDS             complement(284..2095)
                     /locus_tag="ACG8M6_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0457509.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2092..2457)
                     /locus_tag="ACG8M6_001843"
     CDS             complement(2092..2457)
                     /locus_tag="ACG8M6_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0457510.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2620..3015)
                     /locus_tag="ACG8M6_001844"
     CDS             complement(2620..3015)
                     /locus_tag="ACG8M6_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0457511.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3033..3995)
                     /locus_tag="ACG8M6_001845"
     CDS             complement(3033..3995)
                     /locus_tag="ACG8M6_001845"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0457512.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3995..4207)
                     /locus_tag="ACG8M6_001846"
     CDS             complement(3995..4207)
                     /locus_tag="ACG8M6_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0457513.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4228..4926)
                     /locus_tag="ACG8M6_001847"
     CDS             complement(4228..4926)
                     /locus_tag="ACG8M6_001847"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0457514.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4946..5488)
                     /locus_tag="ACG8M6_001848"
     CDS             complement(4946..5488)
                     /locus_tag="ACG8M6_001848"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0457515.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5620..6624)
                     /locus_tag="ACG8M6_001849"
     CDS             complement(5620..6624)
                     /locus_tag="ACG8M6_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0457516.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6621..7610)
                     /locus_tag="ACG8M6_001850"
     CDS             complement(6621..7610)
                     /locus_tag="ACG8M6_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0457517.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7607..8413)
                     /locus_tag="ACG8M6_001851"
     CDS             complement(7607..8413)
                     /locus_tag="ACG8M6_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457518.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8579..9535
                     /locus_tag="ACG8M6_001852"
     CDS             8579..9535
                     /locus_tag="ACG8M6_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0457519.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9612..10130)
                     /locus_tag="ACG8M6_001853"
     CDS             complement(9612..10130)
                     /locus_tag="ACG8M6_001853"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0457520.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10218..10367)
                     /gene="rpmG"
                     /locus_tag="ACG8M6_001854"
     CDS             complement(10218..10367)
                     /gene="rpmG"
                     /locus_tag="ACG8M6_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0457521.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10595..11041
                     /locus_tag="ACG8M6_001855"
     CDS             10595..11041
                     /locus_tag="ACG8M6_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0457522.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11236..13131)
                     /gene="asnB"
                     /locus_tag="ACG8M6_001856"
     CDS             complement(11236..13131)
                     /gene="asnB"
                     /locus_tag="ACG8M6_001856"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0457523.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13456..14430)
                     /gene="bsh"
                     /locus_tag="ACG8M6_001857"
     CDS             complement(13456..14430)
                     /gene="bsh"
                     /locus_tag="ACG8M6_001857"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0457524.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(14764..14851)
                     /locus_tag="ACG8M6_001858"
     tRNA            complement(14764..14851)
                     /locus_tag="ACG8M6_001858"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14817..14819),aa:Ser,seq:cga)
     gene            complement(14996..15562)
                     /locus_tag="ACG8M6_001859"
     CDS             complement(14996..15562)
                     /locus_tag="ACG8M6_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0457525.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         4853 a         3267 c         2616 g         4849 t
ORIGIN      
        1 tttttctctt ttgttccata atagatataa caaataataa atgaggtgga ttcaatgaaa
       61 aaatttgtat ctggaatatt agtaggaagt ctggcaacag ccgctgccgt tgctggtttg
      121 gttgcttctg tgaaaaaaac agtgatcgat ccaattgacg aaaaagaagc aatgatcgaa
      181 gaaaaccgta aaaaagcaat gcgtaaacgc gtttctcgtt aataattatt taaaaatacg
      241 catgaagact ttctaaaaaa cagataagtc atcatgcgta tttttatttg tcagtcaata
      301 taagttgtgc tattttatct gcctgtctca gattccatgg atctgtataa ggtctagcta
      361 ctgctgcagt tagccaatcc acaccttcaa caggtattcc atagcaataa aagtcagact
      421 ggatttcccc tgaagcatca atcacgcgat tcgtacctcg ctcaacatct atagcttctg
      481 aatgataact tcctgtatta tctgaaaatg aaaaagggcg aacaagtttg ttttccttca
      541 aattttttat gacaggatta cttgaacgag aaaagcttgt ttgtggcaat cgagcttcaa
      601 tcaaatagcg actgctgatt tctttatctt gtagagaaat aacatagcgg ttgtgctttt
      661 gctgaatatc aaatggcggt tcgatcaaat gaaaaatacc cgcatctatc agtgctgcta
      721 attctttcgt ccgaatgatt ggcggaccga tcgttaaaaa gctatttaga tgagtaaacc
      781 atccccatag tatttctttg tattccttag cagaaaaaac gtgccattca atagcctgcc
      841 ggattggatc acgccagtct tttaatgcat caaatgttga agcaaatgct cctgtgcaat
      901 tgcctttttc cgcttctttt atctgccctt caataaatat tcggctagct tctgaaaagg
      961 gctcattctg ataggcaaga ggagtttcta aatacgacca atcccatcta taagggatta
     1021 gtgctggatg ctttccgaga cactcctctg aagagaggga gagaaaatca tgctcaaaat
     1081 ttttctttgg aatcggcaag tgatattctt cgatgatttt cttgtaataa aacaactcaa
     1141 cgtctttttt cagatagtca aaaaatgttt cgccagtcaa aaatcccgac tgatgccatt
     1201 gctccaggtt ttcatttgtt agtaaccgtg gccatgccat tgctccgcct tgcttttgat
     1261 ttcttcctcg agggtaataa gggagccccc ttcttgatcc actgtataca atcggttcct
     1321 tgcctgatgg atgatagacc agttttccct tttcgttctc gaagcgccct cctcttgata
     1381 ctgtaaacaa tccgacataa tcaaaaaaac ttaagcctag acctcttaaa aagacatttt
     1441 ctttaggcgg tatcttcgaa agatctacat ctgcaggatt agaaggaagc tgatacagta
     1501 aaacattttt ctttgcgtag tctacccaca ctctttcttc ttctgtctca tcattttccc
     1561 agtgaccgct tgctaaaaca agctgatcga tagaatagct ttgctgatct gtctgtaaaa
     1621 gataaccatc ttttcttttg cttacttttt ttactaaaga ataaacaaac tgaatagaag
     1681 ctggaaattc ttcttgcaac tttgagaaaa accagcgttg atagagtccg tataaacatc
     1741 gagtagattg ctgatctttt tccagacgat tgatttcttc tataattagt tgcttatttt
     1801 tggttaccgt ctgttcgata taatctgtag caggtccttt actccattga aacaagttgg
     1861 gtccctgtgt taccaatcct ccactggaca atgtttcatc tgtaaaaagc gtcacttgtt
     1921 gagaaacaga attcattaat aactccggtg gctgattcaa ccgccaaact cgacctccag
     1981 gaccattggg atcgaaaaga aaaagttcaa gagaatgtct gtcctgatta ttatggagaa
     2041 tcctttctgc tacacttaat cctctaggtc cggcaccaat gatccctatt ttcatttgca
     2101 tgcctccatt tcgataaatt ttttcaataa aacagcatgg ttctgatgtt cattttttgc
     2161 accataaaca aaagtaatga ctggatgttc tttcataagt tgactgagtt taaaaatagc
     2221 ttgcttcttt tcttcttcga aaatcaattc ttgctggtat ttctttgcaa atgaagcaaa
     2281 tcgttctggt acatggtcaa aagttttgcg tgcttcatta ctaggtgcaa tttctttcaa
     2341 ccacagatct aactgcgctt tttcttttga aattcctcgt ggccagaatc tatccacgag
     2401 tattctatag ccatcctctt tttcagcatc aaggtaaaca cgttttaatt tgatcatagt
     2461 tgtttccttc tttctacctt ttcgcttagc ttatatatgt agataatagc acaaaataaa
     2521 aaaaatgaac ggcacaacta tccattgcgc cgttcatgtg atcttggtca aaatctaatg
     2581 attgattcgg tttttaaagc tgtcatacaa tcatctgttt tatacttttt tacttttgat
     2641 ttttccagac catccgtcat atccaccctt aagtaaataa atctcttgat aacctgcttt
     2701 acgtaattta tttgctgtac ggatgctcaa acttttcttt tgatcgtaaa tatagacagg
     2761 ctggtctttt cgaatagatc ctaatgaatc tttcaaaaca gtgtaaggga tgtttcttgc
     2821 acctaaaata tgcccagcat caaacacatc tttttcgcga acatcgatta cttgtgcttt
     2881 tctcattcct tctcgaaatt cttcttctgt caaagtcgtt gctgaacgtt tggccattac
     2941 tcgtagatag acttcccaaa ggatgattgc aagcacaatc aatagcaaaa tgatattaat
     3001 tacccataaa accatgtaat gcctccgttt ttttaagcaa atttcaaggc taatgaggca
     3061 gcaccaatga caccagcttc attaccaagc tctgctaatt tgatttttgt tgattctctt
     3121 acttgcggga aagtaaattc tttaaaatat ttctctacac ggctacgtaa aaattctcct
     3181 gctgccgata ctcctccacc taacacgata cttgacggat ttaatgtatt ccccaagttt
     3241 ccgcaagcta aacctaagta gaagcatacg cgatcaacaa ccatcaatgc aaacggatcg
     3301 tcttcttgag ccatttcaaa gatatcttta cttgttactt cttctccgtt gtctagcatt
     3361 gctttcagtt ttgaatcccc tgcgtattct tctgccaaga aacgacctag gcgtaccaca
     3421 cctgtagcgc tagatacagt ttctaaacat ccgcgtttac cgcaagtaca ttcgaaccct
     3481 tcaggatcca cagtgatatg tccgatttct cctccagctc cgctcacgcc gtgaatcaaa
     3541 tttccgccag caacgatacc tccgccaaca cctgttccta gagtaacgaa tacaacgtct
     3601 ggatcgtttt ctccagcacc tttccatcgt tcacctaaag cagcaacatt cgcgtcatta
     3661 tcgatagcga atttgattcc tgttcctgcc tcgatcagtt ttttcacaaa ttgaacttct
     3721 gtccagttta agttatacgc accaataacc gttccagctt cgctgtccac gcttcctggg
     3781 gtacccatgc cgattccgat aaagtcttcg gctttcatat tatataaatt caaacgatga
     3841 ttgattgatt caatgatttc aggaacgata tgcttaccat catccaaaat attggtatcg
     3901 atgctccatt tttgctgaat ctctccttct ggtgttaaaa ttgcaaattt tgctgttgtt
     3961 ccacctaaat caatcccaat aatcttcttg tccattacaa atttcctttc tttcggcttt
     4021 cttcaattcg gtgctctctt tttaatatgc gccacgctgt catgtatgta tcatgatcaa
     4081 tcaaacggct atcatacaga ttacgcaatt cgatagtcat taattcaatg tcatagatgc
     4141 gtgcacccac atagataaag atgccaaatt ttttgaacag ctgctgtaca tcatataagc
     4201 tctccatctt taagcacttc cttcatatta tacaagtaaa ccatattttt ttaaacccag
     4261 taaaatacat attacaaaaa gaaatacaaa aactagtgta gaaagaatcc tttggtgtat
     4321 cgaatagctt gtctgttgtt tcggtagacc caaaacattc cctaacacta atccgccaat
     4381 tagcccaccg acatgtcccc aaacatctac agaggtatcc agaagaccaa aaacaaatgt
     4441 caacacaata aataaaagaa aatgtctgac tagctgctgg atcgctgggt tatgtttaaa
     4501 atgaaaccct aagataaaca acgcaccgaa caaaccgaat atcgaagtac ttgcaccacc
     4561 agacagaaca ttcgcttcgt taaaagcaaa gcttgctgta tttcccatta tcccgctgaa
     4621 catataaatc aagaaaaaac gccaatggcc atagattgcc tcgatttgct gccccataaa
     4681 atacagtatc agtgagttca ttacgaaatg catcagtcca aaatggatga agattggtgt
     4741 cactaaacgc caccattcat tgaagtgaac aagataagga ccatacattc cggcataata
     4801 agggatctca agtcgcggaa ataattccat catgaggaag accgctgtct gtatccctaa
     4861 aaaagcatag gtccataacg gtcgtcttaa ccatacacgc aattttattt gctgttgata
     4921 attcatgaaa acctcccatt tcattttagt caataaacaa ttgttggatc ttctgatcaa
     4981 agctctccgg ttgccattgt tcattcaatt gttcacgaaa aacaaggcta catgtttttt
     5041 tatgaaaatt atttaaatac cggtcgtaat aaccaccgcc aaaaccgatt cgataaccgg
     5101 ctctattgta gataatacct ggtacaatga ggagatcgat catctccggc aaatagagct
     5161 tctctgattg tggctcaaga acaccaaaac tcgaacgtac aagaacggac tgttcatcca
     5221 tttcataaaa cgccatctga cgtttcggca aagttttagg tacaactatt ttcttttgtt
     5281 ctttttctgc tcgttggata accggctgtg tatccagttc aaattcattc gataaagtcg
     5341 tccctatcac ttttgctgac ttccagtact cgctggcgaa tagctgttta tagattcgct
     5401 gttctttttc ctttttcttg ccacttttgg ccagattttt cagcttttcg attcctttcc
     5461 ggcgtaatct tactttcaat tggtccaaaa actcaccctt ccttctctca taaaatggta
     5521 taggaaaaaa agaaaaatag caatcgaact ttcaaataaa tgcataaata gaataaaaag
     5581 aagacctaga aatgattggc cttccttttt taatgaattt caactgttca gcgcgtgttt
     5641 ttgtctgata aaaagtgcat acagaaaata tggcgcacca aaaagggcaa tcaaaattcc
     5701 tgtaggtact tccccactta gtagaatcac tctagcaacc atatctgcac taagcagaaa
     5761 aatggaaccg aacaaaccgc tcagaacaaa ggtccagtta ttttctttac gtacgactag
     5821 cttcgcagca tgaggagcta gtagtcctac aaaagataaa ctacctgtca ccgctacact
     5881 accgcttgcc aaagcaacag ctaacaacaa aaggattttt tgacttttag ccacgttaag
     5941 gcctaatcct tgcgcgcgtt cttgaccaaa tgatagcaat acaatcctct gtgcataaag
     6001 tcctcctagc ggaattaaga taaccgccca tggaagcaat gcccaaacat aaggccagct
     6061 cgcaccccag attgatcccg ctaaccatgc attcacaaat ccataatttt ccttagataa
     6121 ttggatcgtg catagcaatg ttaatgcaga aaggccggca ttaacggcaa taccggacaa
     6181 taagattcga ttcataccca aaaagccatt tcgatcatag gccgcaagat agacacagca
     6241 agcagctaaa acaccaccta gaaaggctac aactagcaat agaaacggca gcgaatagtg
     6301 cgaaaagaaa cctaaataaa gcatcactaa aaatcctgct ccggcattaa tgcccaatat
     6361 actagaatct gccaattcat ttcttgttac tccttgcaag atataaccag aaacgccaag
     6421 acaagctcct gctaataaac tgatcagtaa tcgaggaact cgaaattctc taacaatcag
     6481 cgccaaagaa gatgattctt tccctatgaa cgtggcaaaa agctgatcca ggtaaataaa
     6541 aggcgtacca atcattaaac tacctataga caggatgaca agtacaaaaa gcaagaaata
     6601 gaacgtccaa tatatttttt tcaccgttgt tccctcctta cttgtatcag taaaaacgga
     6661 acgccaatca atgcaatgac taatccaaaa ggtgtttcaa aagggggatt gatcatcctc
     6721 gctgtaaaat ccgccaacat aaccaatcca ccgccaacta aaaaagagac aggtagaaca
     6781 taacgatagt ccgttccaac cattccgcga acaactggcg gaaccaacag cccaataaat
     6841 gaaacaggtc cgactaacga aacggcaatg gctgctaaca ctgcaactag acagaaaaca
     6901 atcaaccgcg ttcgtttagg atgacttcca agagctaatg catgtgtttc actcaaatga
     6961 atcaaagata ttgcttttcc taatgaaaaa gaaaccaaaa cagcaagtat atataaagga
     7021 aacaatactg ctaattgttt ccatgtgata ttggcagctc cgcctacaaa ccaaaaagca
     7081 agatcctgct gcagattaaa gaattgggta agtgcttggc tgatagcagt aaaaaaagaa
     7141 ctaatagcta cgccagctaa aatcagctgt aaaggattca tgcccatttt cgaaaactgc
     7201 aaaaaaccaa atacgagcaa agttacagca aaagaacctg ccaatgacat tagaaacaca
     7261 gaaaaatcag aagctttagg ccaaaaagca aaactaaggg ccattgccaa cgaagcaccc
     7321 gcgttgattc ctagtaagcc tgaatctgct aaaggatttc ttgtcactcc ttgcatcaat
     7381 gcaccgctca atgcaaaaca actccctaca agaaatgctc ccgccacacg aggcaaacga
     7441 atatttcgta tgatttggtg actttgtttt gatctatcaa aatcaaacaa cgcctgccaa
     7501 aaatctgctt ttcccacagt cgctgcgcca tttgaaagag caacaaaaaa aataatcaaa
     7561 agaacaaaac agaccaaaag caggagtcta tttttcttca tagtcctcat aattgatcct
     7621 cctgctcttt tggcaacaaa tcataggtta gaaaaactgg tttatttgtt cttggaagcg
     7681 tgacaatcgt tgcatcaatc tcaaatattt ttttcataca ctcttttgtg atcacttctt
     7741 caggtgtacc ttctgtgatc aacttaccgt ttttcattgc taataaataa tctgaaaaac
     7801 gtgaagcatg attaatgtca tgaatcgaca tcaagatcgt tgtttgtttc atttgattga
     7861 tttcagtcaa taactgtagg atctctaatt ggtgtgccgg atctaaaaat gttgtcggtt
     7921 catccaagat caagatatct gtttcctgag caagcgccat agcgatccag acacgctggg
     7981 tttgtccacc agacagcgtt gcgatatccc gcatacgaaa tccagctaga cctgtagcat
     8041 ccaacgccca ctcgattgct tcatgatctt gcgtgtccat gccactgaac agtttttgat
     8101 aagggtatcg tccgtaagat actacttctt ctactgtcat acctgtaggg tgttcactag
     8161 tttgtgctaa taatgcaatt ttttttgcaa gttcttttga actgtatgta gagatatcag
     8221 agttattgac aatgatcttt cctgatttca caggtagtat acgggtaaaa cttttcaata
     8281 aagtagattt tccacttcca tttggcccaa tcaaactagt gattttccct tcaggaatcg
     8341 taacagacat gttttcaatg atttttttgt tttcgtagcc ggctgcaatg tcagctgctc
     8401 ttaatctcgt catttcaccc atcctttcgc tgaaaatcat tctcaattat gacgctgttt
     8461 caacatttcg tcaagcgcta ctttcatatc tatagccaaa tgaagcggtt gacagcgttc
     8521 gaaatatcag gaataataac caatgagaaa gattctcaat tatgttagga ggaaagaaat
     8581 gaaattaaaa acaaaagtat tcacattatc tagcatattg ctgctggctg gagtactgag
     8641 cgcttgctcc ccctcttcag ataaagaaac agctacaact gccaattcga cgacttcatc
     8701 caatagtact catacagtca cagatacact tggacataaa gtagagattc caaacgcgcc
     8761 caaacggatc atcggcagtt acttagaaga ctatctgatt gcactagggg aaaaacctat
     8821 cgcccagtgg actgttggca gtggttccat tcaagattat ctgcaagatg atttaaagga
     8881 tgtcccaacg atttcttatg accttcctta tgagaaagtg ttaagctttg agcctgattt
     8941 gctgttgatt tcttcttccg ctactgtcga aggcgggaaa tacgaacaat atagcaaaat
     9001 cgcgccaact tatgttgtaa aaaacggaac agatgtcaca tgggaagaac aactaaaaga
     9061 tgtcggcaaa gctttggcaa aagaaaaaga ggcagaaaag atcatttccg attatcaaaa
     9121 atccatcaag aaaactcgag aagaactagc agataaaatc gagaacaaga cagctgcagt
     9181 gttgtgggtg accaacaact cagcctttat ggtcagcaaa aatcggtcaa gtggaagaat
     9241 cgtatacgac gatctggaat ttggtgttcc tgatctagtg gaagaggtct ccaaaaaagc
     9301 cacttctgac tggtctgctg tttcctctga aaagctagct gaattagatg ctgactacat
     9361 catccttgtg aacagtgaca aagatgctgc catgttcaat gaaccaagct ggaagaatct
     9421 taaagcagtg acagacaatc atgtactgca attcggacct gaatctagtt ggctatataa
     9481 cggacctatt gccagtcagg aaatggtcaa tgatatcaaa gaaaaactaa attgatttta
     9541 actaaaaaaa gcatcttcac gattagtcgt gatatgcttt ttttcttatt aacaaactag
     9601 acgcatagat tttattcttc ttttgaagca accctatttt ttactctttc ccatttttga
     9661 aaacgatggg catatagatt ttcttgatcc gttatcccct catactcctc tataagggcg
     9721 aagtctgaaa aatcaatatt cccaatatac gtatcgcctt caaacgtgct atcgatcaat
     9781 gttcgccaga tccgaccagt gtaaggaaga agttcttgaa aaatccgact accacccgaa
     9841 acatagatat cttctgagat cgtttgacta tacgacaaaa gttcatcgat cgaatgcatt
     9901 acttccgcat tttctttcgt gcgaaaattt ggctgcgttg tcaacacgat gatatggcga
     9961 taaggaagtg aaagatcccc cattccttca taggtctttc ttcccatcac taaaattcga
    10021 tccattgtat gttcgcgaaa gaaacgcata tcatttggca atcgccatgg taacaaacca
    10081 tttttgccga tcagaccatt tttatcttgt gcccacatgg aaataaacat attatccgct
    10141 ccttatgatt ttagatgttt tgagataaaa aaagtctcaa ttctttgacc agcaaggaat
    10201 tgagaacttt aaaaacatta ttttgtttca cggtgcaaag taactttgcg ttcgcgtggg
    10261 caatattttt gtttttccaa acgatcagga ttgttacgtt tgtttttgct tgttagatag
    10321 ttacgttctt tacaagaagt acactctaaa gtgatgttta cgcgcatgtt tttccctccc
    10381 atttttacaa gaatcctcta agatgactct cagacttgat tatcttacca tgattttttc
    10441 tagattgcta gtgtttttca cgattttgtt aagtaaaact acttgacgat aaatacaacg
    10501 gttttacttc tcaaacaaaa tctttttctt tttgtataaa tacccttaca cttgtttata
    10561 tagattatgt tttttaataa agggggatac taatatgcca tggaacatga aggactttcc
    10621 tgcatcaatg aaaaacttag ataaattgac aagaaaaaaa gcaatcgaca tcgcaaatgc
    10681 attattagat gaagggtacc ctgataaccg tgcgatccca attgccattg atcaagcaaa
    10741 aaaatggtat gaagatgcta gtgaatcaga aaaacgaact ttcgaaaaag aaaaaaatcc
    10801 aagcaagacg gatgaacacg atacgaatcc tcgtgctgga aaactgcttg attcagatgt
    10861 gatcgtagaa tatgaggaag aacaatggat cgtgaaatct aaaggggcaa aaaaggcaag
    10921 caaccatttt gatacaaaaa aagaagcaat cgaaaaagga aagcaagtag cacaaaacaa
    10981 agaatcaact ttgttcattt ataaaaaaga tgggacaaaa gaaaaagaaa tcgattattg
    11041 aacatatgta aaggggttgt gacgaaaatc gtgtcacaac ccccttatgt tcgaaacaat
    11101 ggtgttcctc tgccaactcc gaaccataag tcataatatc tataaaacgg gaagagctgt
    11161 tttttgaaat tgtttcttat cactcagtgt taaacggtag gagaaaacct cttttttatt
    11221 gatatttgat tttcatcaaa cagtcacata gtcgattgct tctgcttgcg gaatcgtttc
    11281 atctacaaag tagactttat accatgtcaa gaaactgaag attgtccaga tttttctttg
    11341 ctcttcttta cgtccttcgt gatggtcatc tagcaacttc atgatttttt cttgatcaaa
    11401 aaattctttt gcaaaatctg cttcgaacaa tgctttgact tgttcgtagc cgtgttcttc
    11461 tttcaaccaa gctttgattg gcacagggaa tcctaatttc acacgattcg accatttttc
    11521 cggaagatgt ttgttcgcag cttgtctgaa aatatctttt gtgttgtttt gattcaataa
    11581 aaacttactt ggtatttgtt gagccaattt catgacttcg atatccaata aaggtactcg
    11641 gacttccaaa gaacttgcca tagaaagttt atctgccttt aacaagatat cttttggcat
    11701 ccattgatga agatcgacat attgcatctt attgacttca tctttgattc cttctgtttt
    11761 ctcgtaatga acagtcatga tttcatccac agatggtgct gtttgatatt ttggtgttaa
    11821 cacttctgat gcttctgatt cttcgaacac tcgagcatga ccgataaaat attcttttgc
    11881 aggagcaagt gattcatata aatggatccg tccatggaaa tttttcattt ttccgatttt
    11941 acgtcctaaa ttatagcgtg ttccttttgg taattttttc aatccttgag caatcacacg
    12001 gatgaatttt gaattcgtat ggaatccata cgtttggtat cccgcaaaca gttcatccgc
    12061 cccttctcca gattggacta cccgcacact ttgtgaagca agatttgcta aaaagtacaa
    12121 aggtacgcaa gaaggatttg aatcaggttc atctaagtga tactggatca aagggaatgc
    12181 tttgaacgac atgtctccgt cgatcactgc tgctgtatta tctaagtcca atagttcggt
    12241 tagttttctg gcttcaatcg cttcattata ggttttatca tcaaacccga ttgagaaaga
    12301 atgatctggt ttcaatacag aggttacata actggaatca accccgctgg ataagaaaga
    12361 accaacttct acatcactga ccgtatgtgc ttcaatagaa gcttgaacgg tttcgtcgat
    12421 tttttcgatc cattcttgtc gggaatgcgt ttcttttgtt tcaaaatctg catcccaata
    12481 ttgctgaatg tctatttttc catctttata cgtataataa tggccttcag gcaaacgata
    12541 gacatctttg aagaatgttt cgccgtttaa tggagaatat tggaaagtca tataaggttt
    12601 caacgcttct ttgttcaatt ctttgttaaa atcaggatga ggcagaaaac ttttgatttc
    12661 tgaaccaaac ataaatgttc cgttcatttc tgcataataa tatggtttga tcccaaagtg
    12721 gtctcttgca ccgaataact cattcttttc attatcccaa atagcaaaag caaacattcc
    12781 gcggatcttt tgcaataatt ctgttcccca ttcttcgtaa ccatgcaaaa ggacttctgt
    12841 atctgcatgt gtttgaaaaa cgtggccagc tgcaatcaat tcttctctca aaggctggta
    12901 attatagatt tcgccattaa aaataatgat ctttgtccga tcttcattgt aaataggctg
    12961 agtaccgccc tctaagtcaa taatacttaa tcttctgaat cctaaagcaa catgtttatc
    13021 gatatattct cctgaactat taggtccacg gtgaacgatt cgatccatca ttgtattaat
    13081 aattgttttt ttgttgtcct tgtcgttaac aaaacctaca ataccgcaca tttaactttc
    13141 acatcctaat ataaaattac tgtttatttc attcatatgc atcttatcat agaataaata
    13201 gcggacgaat attttctctc ttacaatttc gtcacgtgaa aacaaaagat gaaagtttct
    13261 gtttttacag aaaataaagt cccaatatct aatttaccat gttttctttt ttgaggctac
    13321 cttattctct tcgtgttaaa aaaatattag tggatgctat ttccaaaaaa accagcaaaa
    13381 aaagacacga cctaaagatc gtgtctaatt atgactgtat cagaaaatat gataccaaac
    13441 ggatctattc attaattaat tgacatagtt tatctgttgc tcttttatga ctggataact
    13501 gaccaattca tgacgatcca agtcttcttt attcatattt acagcagtaa tttgactgtc
    13561 ttcatacgta cgataataat aaatcccttt atcaacgtta cagcaagaag aataaatcgt
    13621 atattcgtat tttctttctc cagtatcgca tactcctttt tgttgctcga ctgagcctag
    13681 tatatgaaaa aattgactga tactttcaga ttctgaatca cctgatgcag cattcatttt
    13741 agtaaatgtg gcttttacaa atcgagaaac agaagagaga tctccaggta agcccaatcc
    13801 tcccatccca cgactataaa cattcaagct gatctggttt gaaaaatgat tttctggtgt
    13861 tccatttgat agcgagcgat aattgttcag attaaataat tgataatcaa atggcggatt
    13921 atttgtcaac acaccgacag gattatcata taaatgaagt ccatctttca tactttcaat
    13981 tactatcgat ttttctttat cagctaataa ccagtgtaaa ggtgacaagg gtagtttttc
    14041 actaaaatcg atattgacta gattgatagt agccaataat tctttagctt cgtctatggt
    14101 tgagcattgc cctagtatcc atggaataaa ctcaaatggt gcaacgttat cttttccttc
    14161 ttgtaattct ttataatcag cattgccagg aaaattcagt cctgccatac tcaacccttt
    14221 ttcatttgta gcctcataat agagtggata gttggatacc ccagctgcta ttcctatcat
    14281 tgcataatgt ttatctaaat tttttacctt tctaaaatcg aaacgataat ttcttggagt
    14341 aatagtgacc acttcattat aagacatctc ataatcaaaa ttccttccaa aataatggtc
    14401 tttcgttgca taagtgattg cggtacacat cgtttcccct cctaaaacaa atcgttttta
    14461 cacttttatc ttacatctag tatttttttt atcaaaaaaa ggccaaagaa gattgattta
    14521 ctagcattta taaaaaatag tactcagggg gaatcaaaaa atattgtagg ttaaaagtac
    14581 aacaataagg ggctgtgaca aaagtcttgt cacagcccct tattccgaac aaacggtgtt
    14641 caaaagcttg catctgcggt aataagccca atcaaacgaa aaaataggaa aagctatttt
    14701 tgtttggccg ttcttattac ttgatgcagg acagcttctt cacaacctct aatctttgat
    14761 ataaggaagg tacaggattt gaacctgcgc gccggtatta gccggttcgc cggatttcga
    14821 gtccggtgca ttaccactct gccaaccttc cgtaagtact atttttaaac ttttttcacg
    14881 aaatgtcaag cctatttcta atagataaat aacagaaatc gcttttttta gcaaaaaagt
    14941 ccattgacag tttgcagcca acggacttac cgatcaaatt catactgata gaacactatc
    15001 tgattatttc aggattatct aatgctaaaa tcgagtcaat tgtccgtttg atcagtgcaa
    15061 tgatttgttc ttctgttgga tgcatgatgt ttgatgacat caatgacgct aagccagtca
    15121 ctgctaccca tgtaccagta tgcagagcat cgacatattc atccgataat tgattgtatc
    15181 tttcatcttc tttaactatt tcacggaaat aattgtatga aaaatcatgc attttctttc
    15241 cgtccccatg ttcatcaatg tacaatgcca tgtaaagact atgatgcttt tttgcaaagt
    15301 agatatagtt caaaccgaga tcaatgattt tatcaccagt acgttcaata gggaaaacat
    15361 cttcttttag ttgttcaaag atagaatcca gcaacgtatt ttttaaatct tccatgtttt
    15421 tgaattccag ataaatcggc tgtgtcgaaa tccccatctt tttagcaata tttctcgcag
    15481 taaatccttt aaacccttcc tttgcaacaa catcataagc agcttttaga atttgatcct
    15541 tggtatagac ttttcttctc attaaaactc ccccacttct ttact
//
LOCUS       DAZKOB010000061        15383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000061 DAZKOB010000000
VERSION     DAZKOB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.061"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..410)
                     /locus_tag="ACG8M6_001860"
     CDS             complement(45..410)
                     /locus_tag="ACG8M6_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457526.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            618..1100
                     /locus_tag="ACG8M6_001861"
     CDS             618..1100
                     /locus_tag="ACG8M6_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457527.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1297..2187
                     /locus_tag="ACG8M6_001862"
     CDS             1297..2187
                     /locus_tag="ACG8M6_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0457528.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2512..4983
                     /locus_tag="ACG8M6_001863"
     CDS             2512..4983
                     /locus_tag="ACG8M6_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0457529.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5086..6822)
                     /locus_tag="ACG8M6_001864"
     CDS             complement(5086..6822)
                     /locus_tag="ACG8M6_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0457530.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6819..7523)
                     /locus_tag="ACG8M6_001865"
     CDS             complement(6819..7523)
                     /locus_tag="ACG8M6_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0457531.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7654..8157
                     /locus_tag="ACG8M6_001866"
     CDS             7654..8157
                     /locus_tag="ACG8M6_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0457532.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8117..8455
                     /locus_tag="ACG8M6_001867"
     CDS             8117..8455
                     /locus_tag="ACG8M6_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0457533.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8476..9894
                     /gene="ffh"
                     /locus_tag="ACG8M6_001868"
     CDS             8476..9894
                     /gene="ffh"
                     /locus_tag="ACG8M6_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0457534.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10006..10584
                     /locus_tag="ACG8M6_001869"
     CDS             10006..10584
                     /locus_tag="ACG8M6_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0457535.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10588..11109
                     /locus_tag="ACG8M6_001870"
     CDS             10588..11109
                     /locus_tag="ACG8M6_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0457536.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11188..11742)
                     /locus_tag="ACG8M6_001871"
     CDS             complement(11188..11742)
                     /locus_tag="ACG8M6_001871"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0457537.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11995..12270
                     /gene="rpsP"
                     /locus_tag="ACG8M6_001872"
     CDS             11995..12270
                     /gene="rpsP"
                     /locus_tag="ACG8M6_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0457538.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12282..12533
                     /locus_tag="ACG8M6_001873"
     CDS             12282..12533
                     /locus_tag="ACG8M6_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0457539.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12658..13449
                     /locus_tag="ACG8M6_001874"
     CDS             12658..13449
                     /locus_tag="ACG8M6_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457540.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13619..14140
                     /gene="rimM"
                     /locus_tag="ACG8M6_001875"
     CDS             13619..14140
                     /gene="rimM"
                     /locus_tag="ACG8M6_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0457541.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14130..14891
                     /gene="trmD"
                     /locus_tag="ACG8M6_001876"
     CDS             14130..14891
                     /gene="trmD"
                     /locus_tag="ACG8M6_001876"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0457542.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15027..15374
                     /gene="rplS"
                     /locus_tag="ACG8M6_001877"
     CDS             15027..15374
                     /gene="rplS"
                     /locus_tag="ACG8M6_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0457543.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
BASE COUNT         5115 a         2783 c         3172 g         4313 t
ORIGIN      
        1 aaaaagaaga aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct
       61 tttttataag ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata
      121 cctgcaagaa gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca
      181 gtaaaattgg cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa
      241 gatataatac gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat
      301 gtgatgaacc cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa
      361 ggaacaaaag ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac
      421 cacctaacaa gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa
      481 ttactttttc attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa
      541 aaagtttttc cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt
      601 agtttggggg aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt
      661 ggttttttcc tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg
      721 agatattagg cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga
      781 ctgttttcta tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact
      841 acaattggca gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc
      901 tgcctcgctt gattatcggc cctaatggtt tgagcgagag tggattgaag gattcagctt
      961 attcctccat gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta
     1021 ttcttgcaac atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat
     1081 ctataaaaaa taactattga tggtggatca tcagtaaaaa agcatatgag ctgcgtaaaa
     1141 tgcatgtttc tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg
     1201 atttttacat aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg
     1261 ataaacttta gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta
     1321 ggtatgatgg gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct
     1381 gccacacaaa ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg
     1441 acagcattac agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat
     1501 tatcaaatct ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga
     1561 gcagttacta tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca
     1621 aaattagtgg atcataaagt agcagtggtc gggatggcgc caatcgtcaa taaagataca
     1681 gatgtcaaag atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac
     1741 tggaaggaag ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc
     1801 ggtacccgcg cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa
     1861 gaacaagatt catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc
     1921 tatttagctt tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa
     1981 ccaaaagaag agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac
     2041 acgaacggaa aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa
     2101 atccaagaag gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa
     2161 cgtgatcacg aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact
     2221 attaaggaaa aatcaactat gtttcccaca tatacatttt tcctttaatt gtgaaaaata
     2281 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt aaacaccgtt
     2341 tattcgaaaa aaagagctac gacatagaac tgtcgtagct ctttttttgc tatatgacag
     2401 gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt ccatatctgt tctatactgg
     2461 ataggagaag tttggaaata tccagaaaac gaaatcaatc gaggtagcca catgaagaat
     2521 aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc
     2581 cgtctttttc catcgtataa agaaggatta acggcagatc aagtcaagga acgaatagaa
     2641 aaaggtgcag caaataattc agtcgatccc acattcaaaa caaatcagca aatcgtattg
     2701 gaaaacatat ttacgtattt caatttgatc tttcttatct tagccatttt gctttgttta
     2761 gtagaatcct ataagaactt gactttttta ccagttatca tagccaatac aggaatcgcc
     2821 atttttcagg aaattcgatc caaaaagatc ttggacgaac taaatgtttt aaatacagca
     2881 aaagtcaatg tcatacggga cgggatagaa caaacaatag atatagaaga ccttgtaata
     2941 gatgacattg ttattttaga gactggacat caaattccag cagatgcaga ggtcgtggaa
     3001 ggaaaactgc aagttaatga ggctttattg acaggcgaag cagatgaaat aaccaaagaa
     3061 ataggcgatc cattaatgtc cggcagtttc attgtttccg gaaaagctta cgcccgatta
     3121 gacaaagttg gagcagattc gtatatttcc cagctgacag tcaaagccaa gacaatggga
     3181 gaaggagaac agtcggagat gattgcttct cttaatcaat tgatcaaatg gattgggatc
     3241 atcattattc ctatcggtat cctgcttttt tctcaaagtt acttttttaa tggaaacacg
     3301 atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg gtatgatacc tgaaggactg
     3361 tacttgctga cgacgattgc attagcatta agtgcgacta aactagcaaa gcaaagagtt
     3421 cttttacaca atatgaaaag cattgagaca ttggcaaggg taaatgtcct ttgtgtagat
     3481 aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa aagtgatcgt ctctaaaaaa
     3541 caagaaataa tcagtcagtc agatcaattg gaagcatgca ttgctgacta tgcaaaagcg
     3601 atggcaggag acaatgcaac aatggaagct gttaaagcat attttacaaa aaccactgat
     3661 caatcctatc accatgttat tcctttttct tctgtccaaa aattcagcag tgtagcgttg
     3721 gcagacaagg tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca attgccggaa
     3781 tatgcggaag agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg
     3841 tacgatggga ttcttgaaga accagtattg acagaagcag tcacaccatt aggctatatt
     3901 ctgattgcta atcctatcag aaaagaagca agagccacat ttgattactt caaaaaacag
     3961 caagtagcca tcaaagtgat ttctggagat aatccgctca ccgtatccaa tgtagctgtc
     4021 caagcaggga tttcacatgc tgatcagtac gttgatgcat ctaaattatc agaagaagaa
     4081 tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga acaaaagaaa
     4141 atcttcgttc aattgataaa gcgtaacaat acagtagcta tgacaggtga tggcgtgaat
     4201 gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg aaatgaagcc
     4261 gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat gccagaagtt
     4321 gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt gtttttagtg
     4381 aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct gacctatcct
     4441 ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc atctttcttg
     4501 cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa
     4561 aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt gatctgcggg
     4621 gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct tttgattgca
     4681 gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag aagtcagatc
     4741 attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa gctgttttca
     4801 atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt ctcggcagat
     4861 tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa
     4921 gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa acgagaaaat
     4981 tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc
     5041 aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca ttttcggaat
     5101 ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg taccattaag
     5161 gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg aatgtcggct
     5221 tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct caatgtcgat
     5281 acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat agcggataat
     5341 aatctcgctg tcgctttttg aatattggac agcattttca atcagatttt tgataattgg
     5401 ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa
     5461 ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat aaggttctgt
     5521 ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga tgatttcccg
     5581 aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt
     5641 ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca gtgaagtcac
     5701 aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct tttctaaacg
     5761 tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt ctgaattagt
     5821 aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga tctcctgatg
     5881 gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg gctcttgaat
     5941 gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta gctgcatctt
     6001 tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc caatcatcag
     6061 ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat acatcttact
     6121 catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat cgggtgtttg
     6181 catgattact tccgcctgat taggattttg caccattttt tttagtacgg gcagcacggt
     6241 tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa gaacaaggaa
     6301 gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg gtaaaaaacc
     6361 gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt tgactggtaa
     6421 tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc tacctaggtt
     6481 cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat tcaatgtttc
     6541 attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc gttcgttcga
     6601 ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt tttcgacatc
     6661 taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct gttccacgat
     6721 ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac taactaaaag
     6781 aataagaaag aagagacgtt cataaatttt tttccatttc atcgttttgg ttcctgaaac
     6841 ttatagccaa atcctctaat agtcaccaag tactttggat gttttggatc tcgctcgatt
     6901 ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa
     6961 ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa
     7021 taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc tacggtcact
     7081 tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg ctcagcttca
     7141 tcttttgatt gcttacgagg ttcaatccgt ctgaatattg ctttcatacg ggctaatact
     7201 tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag gccaatgatc
     7261 cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt tttttcttga
     7321 cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc taaaatgatg
     7381 aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt ggcactggtt
     7441 accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga aggttcatca
     7501 tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt ctgtttatat
     7561 tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt ataataggaa
     7621 ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc atgtttaggc
     7681 ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat ggaactagtt
     7741 gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc aattcctaga
     7801 gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta
     7861 tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat
     7921 caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag cccttcatgc
     7981 ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaaatgg tgccggagtg
     8041 gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa
     8101 actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt
     8161 ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt atgcagatga
     8221 tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag tatatgacaa
     8281 tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaactccatc ttttttctaa
     8341 ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag ataagtaccc
     8401 ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag aataaataaa
     8461 aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca ggcgatggga
     8521 aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc
     8581 cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt tactaagcgc
     8641 gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc
     8701 gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc agagttaaat
     8761 aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc aggtaaaacg
     8821 acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg tccgctccta
     8881 atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt aggtcaacag
     8941 ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat cgtccgtcaa
     9001 gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac ggcaggacgt
     9061 ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca
     9121 aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa cgtagctgat
     9181 agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga tggggatact
     9241 cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa attcatcggt
     9301 tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc tagccgtatc
     9361 cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta cgacgagaaa
     9421 aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt
     9481 gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa aatgatccca
     9541 ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc
     9601 aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga tctgttgaac
     9661 ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga agtgaatcga
     9721 atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa aggggatatg
     9781 aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg
     9841 gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa
     9901 aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta tcagcaattt
     9961 ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag atgtccgtgg
    10021 agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt tcctgaatat
    10081 gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg attgagctgg
    10141 cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt cgatatcaaa
    10201 aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa aggaattatt
    10261 cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt gaaaattcaa
    10321 gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa aatcgttgat
    10381 tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga tgaattagca
    10441 aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta tgctttttta
    10501 gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga agaggtcgta
    10561 cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg tgctataagt
    10621 ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga cactccaaca
    10681 ggtaagcaaa ctacttatga agctttttat gaaacaatcg atacgaccat catgggcaga
    10741 aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc agagaaaaag
    10801 aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt cgtaaacgaa
    10861 gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat ctgggttgtg
    10921 ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga atggtatatc
    10981 cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg aacatatgaa
    11041 gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt gcattatgta
    11101 cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc ctgtacatag
    11161 ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa aatctagata
    11221 cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac cattttcatc
    11281 aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg gcacattcaa
    11341 aaagacaagt gattggcgta gatggtggtt tgcgccaaag ccgccgatcc cacctggtga
    11401 tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag gtcttgagcc
    11461 tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag gagtaacgaa
    11521 aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag caggagcctg
    11581 ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta caaatacact
    11641 ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt tgtaagaatc
    11701 ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt tcctccttca
    11761 aactatctat aataatctta caaaagataa gtaactggag caaataaaaa cacttactga
    11821 caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct aaaatcttct
    11881 attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt acagggtaaa
    11941 aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata aataatggca
    12001 gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg tatcgtcgta
    12061 gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac ttacaatcct
    12121 ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg gttatcaaaa
    12181 ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt tatgcaaaaa
    12241 caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat ttaagcgact
    12301 tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag ttagacgtcg
    12361 tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat attggccgta
    12421 tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac ggtgttcgga
    12481 tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag taaataaacg
    12541 tgctgtactc gaattttcga gtgcagcttt tttgtttttt ttattaattg aagaaatgat
    12601 tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga acagccaatg
    12661 agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag ttcggttact
    12721 caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac cagtccagaa
    12781 aaaagtagta ccaaaacggc ttccaccact gaacaaacaa tggctacctc aaataatgag
    12841 aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat gcctctagat
    12901 gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca aggttattct
    12961 attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa
    13021 acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga aacaatccaa
    13081 gttttgcagc catttgagat cgacacaaat ggtcagcaag tggacttagg ttctcgaatc
    13141 acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga aggaaactgg
    13201 cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt agctaaagaa
    13261 atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg taaaattact
    13321 gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat
    13381 gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt ttctatgaag
    13441 aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc acaggaagta
    13501 atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa aagaaaacta
    13561 gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg gatataagtt
    13621 gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg
    13681 tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg tgctaacact
    13741 attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa
    13801 ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa aatatcggga
    13861 tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg aattctatta
    13921 ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta aagtaaaaga
    13981 aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat
    14041 attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg
    14101 tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact ttatttccaa
    14161 gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat aaaggattat
    14221 tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag acagtagatg
    14281 attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc tatgataaca
    14341 tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt cttgacccag
    14401 ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa cacttagtat
    14461 tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg acagatgaag
    14521 tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc atgatcgatg
    14581 caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa acggattctt
    14641 attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat aaagggatga
    14701 aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg caattaaaag
    14761 aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt gaatggacac
    14821 ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa acagcagatg
    14881 aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg ttagtatttt
    14941 atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa gctcataaga
    15001 atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta acaaaagaac
    15061 aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag
    15121 ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc aaacgccgcg
    15181 gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg ggcgttgaac
    15241 gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc tacggtaaag
    15301 tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct cgtatcaaag
    15361 aaatccgtcg ttaatcatcg tta
//
LOCUS       DAZKOB010000062        15249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000062 DAZKOB010000000
VERSION     DAZKOB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.062"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1023
                     /locus_tag="ACG8M6_001878"
     CDS             <1..1023
                     /locus_tag="ACG8M6_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0457544.1"
                     /translation="KISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLI
                     TGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFF
                     FTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGM
                     IISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRS
                     VFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNI
                     LLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLT
                     VRRLKR"
     gene            1020..1787
                     /locus_tag="ACG8M6_001879"
     CDS             1020..1787
                     /locus_tag="ACG8M6_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0457545.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1887..3455
                     /gene="liaX"
                     /locus_tag="ACG8M6_001880"
     CDS             1887..3455
                     /gene="liaX"
                     /locus_tag="ACG8M6_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0457546.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3752..4072
                     /locus_tag="ACG8M6_001881"
     CDS             3752..4072
                     /locus_tag="ACG8M6_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0457547.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4072..4422
                     /locus_tag="ACG8M6_001882"
     CDS             4072..4422
                     /locus_tag="ACG8M6_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0457548.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4589..5527
                     /gene="hprK"
                     /locus_tag="ACG8M6_001883"
     CDS             4589..5527
                     /gene="hprK"
                     /locus_tag="ACG8M6_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0457549.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5542..6372
                     /gene="lgt"
                     /locus_tag="ACG8M6_001884"
     CDS             5542..6372
                     /gene="lgt"
                     /locus_tag="ACG8M6_001884"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0457550.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6416..7441
                     /locus_tag="ACG8M6_001885"
     CDS             6416..7441
                     /locus_tag="ACG8M6_001885"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0457551.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7456..8394
                     /gene="galU"
                     /locus_tag="ACG8M6_001886"
     CDS             7456..8394
                     /gene="galU"
                     /locus_tag="ACG8M6_001886"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0457552.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8519..8959
                     /locus_tag="ACG8M6_001887"
     CDS             8519..8959
                     /locus_tag="ACG8M6_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0457553.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8962..9564
                     /locus_tag="ACG8M6_001888"
     CDS             8962..9564
                     /locus_tag="ACG8M6_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0457554.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9627..10730)
                     /locus_tag="ACG8M6_001889"
     CDS             complement(9627..10730)
                     /locus_tag="ACG8M6_001889"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0457555.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10940..11941
                     /gene="ccpA"
                     /locus_tag="ACG8M6_001890"
     CDS             10940..11941
                     /gene="ccpA"
                     /locus_tag="ACG8M6_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0457556.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12296..12691
                     /locus_tag="ACG8M6_001891"
     CDS             12296..12691
                     /locus_tag="ACG8M6_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457557.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12691..12942
                     /locus_tag="ACG8M6_001892"
     CDS             12691..12942
                     /locus_tag="ACG8M6_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457558.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12985..13380
                     /locus_tag="ACG8M6_001893"
     CDS             12985..13380
                     /locus_tag="ACG8M6_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457559.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13377..14633
                     /locus_tag="ACG8M6_001894"
     CDS             13377..14633
                     /locus_tag="ACG8M6_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0457560.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14645..14968
                     /locus_tag="ACG8M6_001895"
     CDS             14645..14968
                     /locus_tag="ACG8M6_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457561.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14961..15227
                     /locus_tag="ACG8M6_001896"
     CDS             14961..15227
                     /locus_tag="ACG8M6_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457562.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5240 a         2580 c         3223 g         4206 t
ORIGIN      
        1 aaaattagca tcatcactat attttttatt tgtgtgctta cttcatgggt tattcagcat
       61 caagccgctt tatatgaagt acctgtcatc gagatagaaa aagtacgaat aaaagaggat
      121 catcaattga ttacaggaaa actgatgaac tcggatcaaa aaggagaatc gatttattta
      181 tcggttcctt atcaaaaaaa tcaaatagaa acagcaaata taaaagcgag acaaatgtat
      241 ctagtccaaa tgcatggaga tgaatggcag ctagtagcaa aaaaaaatga tggctggctt
      301 ttcttcttca caagtatctt cattggcgcc atgttgctta ttggcggaaa acagggtcta
      361 tttgctttag gaagtctcat catcaatatc gttttacttt tacttttgtt gctcatcttt
      421 actaaaacag aagggatttc tctattaaat aaatctatcc tgttcgtcgt tatagggatg
      481 attattagca tattggcttt agatggatgg aatagaagca gtataatcaa aatttcagct
      541 gcagttacga gtgttttttt agcatttttt atttgtcttt tgacaatgaa tcattggaaa
      601 gaccaaggac tgcgctatga ggaactgaat tatctgacac gaccgtatcg ttctgttttt
      661 ctttctagcg tactgattgg aacagctgga ggaacactcg ataccgttat cacagtcatt
      721 gcgacattag aagaattgac taagcgacaa ccagaaataa cagcgaaaca gttatggctt
      781 tcaggaaggc aagtgggtcg agatgtgatt ggctcgatga ccaatatcct tttgttcgtc
      841 tatatcagtg ggtcagttcc atcgcttctt ttatatttag gaaatcaatg gtctttcaaa
      901 gaaacgatag aacagcattt atcattggaa tttttacgcg tcattgctgg cagcttagga
      961 atagtattat cgatccccat ctcggtcctc ttttttctga ctgtaaggag gctgaaacga
     1021 tgaatgtact tattatactt gttgggattt tagcaatcag cgtgctactt attggcggta
     1081 cgcaaggtat gtatattctt ttaggcttat tcatcaattt agggatcttt ttcctgttgt
     1141 tattcggtta tcatcagaag tggccgatac tggcattgtc ggtcatagga tttttattga
     1201 ttgctgtggt tatcttattt tttatcaacg gttataatct aaaaatgcgt gcagcttttg
     1261 tgagtatact gatcttttta ttttgtttcc tactattgct tcctatcact gattttttag
     1321 cgatccaagg ttttacatcg gtagaactag aagaattatc tggcttagat aagactttag
     1381 cgattgattt tagattactt acgagatcgt tgcttctgat tagtcttagt ggtgctgttt
     1441 tagatgcgag tgtagcaatt tctagtggga cattcgaagt atatcaggca aatccgcaat
     1501 tatcttttta ccaattgcgc cattctagtt tcgctattgc taaaaaaata ttggcgtcaa
     1561 cagtaatgac acttcttttt gcattcatgg gaagttcttt agcgttgatc ctctggttta
     1621 ttgatttaga cattcctttt cagcaagtga tcaatgaaaa atcatttgtt ctagaatata
     1681 cgatggctat tcttaccaca ttgtccgcac taattgttct tcctttaacc tgcgtgacta
     1741 cagcatatct ctttacaaaa aagaaagaac aactcaagat ggagtaaaag ttacaactta
     1801 agtcctatga catttttact gaatcagtgc ataatatgga tgtaaggaaa agcttgcaaa
     1861 caattttgct taaggaggca atttccatga aagaaagaga acgtatttta gatttagtga
     1921 aaaaaggaat cctttcgact gaagaaggct tagatttatt agaaagcatg gcaactgaaa
     1981 aagacgaaaa gcaaatcaaa aaagaagcag acaaggtaac tgctagtcat aaagaaaaag
     2041 ataaggcaag tcaattgatc gataaacttg aaaacggcga agaagaaatt tctgagccag
     2101 ttgatccaaa ggaaagagaa agacaagatc aagaaaactt agaaaagatc ttagatgaac
     2161 tagcaactga agcgaataaa acttcggcgc gtctagatga agtaaatgct caattcgctg
     2221 acaataaggc agcacgcaat gagaaacagg aagcattgat gcagctgaat acaaaagaag
     2281 aattaggcga gttgacagaa gaagaattag cacagcgtca ggcgttagag gcagaaatca
     2341 aagagttaga agctagtggc gatacactgt tagaagaaca attgaaatta gaagcagaat
     2401 taaaagatat ccgtaagaat caatggtctg agaaaaaaga aacgtttact gataagtttg
     2461 aattgccaga tgattggaaa gatcaagcaa cagatacact gaatcaagta ggcgaaaaaa
     2521 tgtcagaagc tggatcacag ttaggcaaat tcttgaagaa aacattccaa actgtttcag
     2581 aaacagtgaa tgacaacatg gaatggaaag atgtcagctt acgtgtccct gggattgcga
     2641 caacgaaatt tgagcatgaa ttttactatg aagctcctgc agcaagtatc cttgatatca
     2701 aggcagcaaa cggaaacgtg acgttaaaaa cgtgggacag caacgatgtg aaagttgaag
     2761 ctaagatcaa actttacgga aaaatgggtg ctgaaccatt tgaagcattc tctgaacgga
     2821 gccagattga ggttaatgaa gatcatatct ccttccaaat tccaaataaa cgtgtgcgtg
     2881 cagaccttgt tttctactta cctaaacgag tatacgacca cgcagctatt aaacttctaa
     2941 atggcaacat catgattgaa acattggaag ctaaagatat ttatacaaaa tctacgaatg
     3001 gtaacatcat cgtggaccaa ttaactgcaa cgatgctgga agtagaaggt gtaaacggca
     3061 atatcgatat tcgaaacggg aacatcttag attctattat cgaaacagta aatggtacag
     3121 tgacatttgg tgcgacagca gaaaatcttt ctgtgtcttt agtaaatggt gatgtacgtc
     3181 ttacgattaa agaagataat ttgaaaaaag tagaagcaag ttcagtgaac ggaaatgtca
     3241 aagtcgcttt accagatggc atcggtttgg aaggacatgc taaaacaagt cttggaagta
     3301 tcaatagccg tctgtccaat tacgaagtag ttcgtgagaa aaaagaacgg acaaaccaaa
     3361 tgttgcaatt ccgtcgtgtt tcagatgatg agatcgcgca agtgcaactt tcaacgacaa
     3421 caggaagtat ctatttaaaa gatacagata aataaagagg ttgtgaaaga gatgttcagc
     3481 cttgagcaat aagaagcgat tttgtaaaat agcttcccct attttacaaa attgtgcctt
     3541 attgccgata gtctatctct tgaacaccgt ttaccaagag attatgaacc tgccaattat
     3601 cgccgagtca caaggaataa tttcagaaaa agctcgtcac gttttatgaa tatggtaatt
     3661 tgtgaccgag aattttgcag aaagacaccg attaagaaga aggttgtcac aacgattttc
     3721 ttcatttaaa atagtagaac agaggtgaga catgatgaaa aaaaagttga caaaatctaa
     3781 taaaaatgtt gttttgacag ggactttagc aggaatagga gaatacatcg ggattgatcc
     3841 gactgttatc cgtgtgcttt atgtattcct gagcttagtt gcttttggaa gtccaattat
     3901 tttatacatt ttattagctt tgattatccc atctgcgcgt tctgcacgaa caaactatgg
     3961 acataacaat gcatattatg aaaaaaacaa ttatcgtgaa caaactcgtg aacaaacaaa
     4021 aaaacgcaaa gaagcggaaa aaatcgatga tgacgaatgg agtgactttt aatgtcgtac
     4081 tttcaacgta ttttagttaa tacgttaaca ttcatatcat tggctgttct ttttcctgcg
     4141 atggtccatg tgagcagtat ttggacggcc gttcttgcca gcttcgtatt gtctgtgttg
     4201 aatatgtttg tcaaaccgat tttgacgata ctgtccctgc cctttacatt agtcacattg
     4261 ggattgttta cgtttgttat caatgcagcg attcttaaaa tgacctcctt ttttgtggga
     4321 gcagaacatt ttagtttttc ttcattcggt gcagcgttgt tagtagctat cttgatgtcg
     4381 cttgtcaatg taattgtcag cgaacataac agtagaaaat aaaagaaagc tgtgccgaac
     4441 tatcggcgca gtttttttat ttagaatcag gaatgcgttt tctattacat tggtaattaa
     4501 ataaaaacgt tttttttaat tggtagtact tttggctaga caatgataaa atgatagaga
     4561 acactctaat agaaatgagg tagcatgaat gtcagaagtt gtaaaagtta gccaattagt
     4621 gaaaaaatta tctttggaaa tcgtgacagg cgacgagaaa agcttagacc gtgtcatcgt
     4681 aactggggac atctcacgcc caggactaga gttgactggt tattttaatt attattccca
     4741 taatcgttta cagctatttg gcagtaaaga aatcagtttt gcggaacgga tgatcccaga
     4801 agagcgtttg atcatcatga gaagattatg tagtgaagat atgcccgcat ttattatttc
     4861 aagaggatta ccagctccag aagaaatgat ccaggcagca aatgaaaatg ggatagctgt
     4921 actgcgttct cccatctcaa cttctcgttt attaggagaa ttatccagtt atttagatgg
     4981 tcgccttgcc cctcgaacaa gtgtccacgg tgttttagtg gatgtttacg gattaggtgt
     5041 actgattcaa ggtgacagtg ggataggaaa aagtgaaaca gcgttggagt tgatcaaacg
     5101 cggccatcga ttgatcgcag acgatcgagt agatgtttat cagcaagatg aattaacggt
     5161 gatcggagaa ccgcctaaaa tcctggaaca tctgattgaa atccgaggaa tcgggatcat
     5221 tgatgtaatg aatctttttg gtgcgagtgc ggtacgtggc tctatgcaag tccagttggc
     5281 tgtttatctg gaagcatggg caaaagataa gaaatacgac cgtctgggct ctgaagacac
     5341 gaccgttgaa atcgcggaag tgggcattcc acaaataaaa atccctgtca aaacaggacg
     5401 aaacgttgca atcatcattg aagtagccgc gatgaatttc cgtgcacgta caatgggctt
     5461 tgacgcaacg aaaacatttg aagaaagatt aagtcgcttg attgaagaaa actcaggcaa
     5521 cgactagcaa ggagtgacaa tatgttagca caaatcaatc gagttgcttt tgagcttgta
     5581 gggattccta tctactggta tgcattgatc atcgtatcag gtatcattat cgctatgtgg
     5641 cttagcagca gagaggctgt tcgggtagga ttaaaagcag atgatgtgac agattttatg
     5701 ttgtggggat tgcctatctc cattattggc gctcgcttat attatgtttt atttgatctt
     5761 ccccagtata tagctgatcc aatccaaata ttcaatatcc gttcaggagg cttagctatt
     5821 tatggcggac tgattgcagg cgcgatcacg ctgtattttt ttactaaata tcattttatt
     5881 tctttgtgga cttttttaga tatcgctgca ccaagtgtat tattagcaca ggcaatcggt
     5941 cgttggggaa atttcatgaa ccacgaagca tatggaccag acacgacgcg aagcttctta
     6001 gaaagtctgc atttaccgca attcatcatt gataatatgt atatcgaagg gatctatcgt
     6061 caaccaacat ttctttatga atcactttgg agtttgattg gatttatttt gcttttgttt
     6121 ttacgaaaga aaccacacct tttgaaaaaa ggcgaagtcc ttttatgtta tctaatgtgg
     6181 tatagtttcg ggcgtttctt tattgaagga ttacgtacag acagtcttta ctttttaggt
     6241 gtgatccgta tttctcaatt attatctgct gtgttatttg tcggtacatt gattctattt
     6301 atttggcgca gaaaaaaagg aaatttaccg gattatgacc gctcattagg aaaaaatcaa
     6361 caaattatct gatttttaga gcgttaaaaa tgaaaaaaat ttcaggagtg gtaaaatgaa
     6421 ttatagagta gcggttttag gaccaggctc ttggggtact gctttggcac aagtcctcgc
     6481 cgaaaacgga catgacgttc ggatttgggg acatcgtgct tcacaagtag aagaaatcaa
     6541 cacccaacac acgaatcaac gttatttacc agatcattat ctacccgatt ccatccaagc
     6601 attcaccaat atggaagatg cagtaaaaga cgcagatgcg gtgctattcg tcattccaac
     6661 gaaagcaata cgttctgttg caaaagaact agtgcctaaa ttgaatacaa agccagtgat
     6721 catccatgca agtaaaggat tagaacaggg aacacataaa aggatctctg acatcttgtt
     6781 agaggaaatc ccagcagaaa aaagaaaagc tgttgttgtt ctttctggac caagccatgc
     6841 agaagaagta gcaaaacatg acatcactac gatcacagct gcaagtacag atgcaaaagc
     6901 agcaaaatat gtacaagagc tgttcatgaa taattatttt cggatctata ccaatcctga
     6961 tgtgatcggt gtagagatgg gggcagcttt gaaaaatatt attgctattg gtgctggcgc
     7021 tattcacggc ttagggtttg gcgataatgc aaaggcagca atcatgacgc gcggattagc
     7081 tgagatcagc cgtttaggtg tagcaatggg tgccaacccg ctgaccttta tcggattaag
     7141 cggaataggc gatctagtcg ttacttgcac aagtgttcat tctaggaact ggcgcgcagg
     7201 caacttgtta ggacaagggc agcccctaga agaggtgcta gccaatatgg gaatggtcgt
     7261 tgaaggtgtg aatactaccc aagcagcgat ggaactagcc caaatgatgg aaatcgaaat
     7321 gcccatcact caaacaatct atgaagtgct gtatgaacaa aaagaaatca aaacagccgc
     7381 aaaagaaatc atgttacgtg atggaaaaaa agaaaatgaa ttctcattaa caattttttg
     7441 aaggagcgaa tgtatatgaa agttaaaaaa gcagttattc cagcagcagg attaggaaca
     7501 agatttttac cagcaacaaa agctatggca aaggaaatgt tgccaattgt tgataaacca
     7561 acaatccagt tcatcgttga agaagcgttg aaatcaggta tcgaagacat cttgatcgtt
     7621 acaggaaaag ctaaacgacc aatcgaagat cactttgatt caaatattga attagaaaca
     7681 aacctaagtg aaaaaggaaa gaccgaatta ttgaaattag tagaagaaac gacagatgtc
     7741 aacttgcatt tcatccgtca atctcatcca aagggtttag gacatgccgt tcttcaagca
     7801 agagccttta ttggaaatga accatttgtc gttatgctag gcgatgatat aatggaagac
     7861 gaagtaccat tgacacagca attgatcgat gactatgagc gtacacacgc ttctacgatt
     7921 gccgttatgc aagttcctca cgatgaaact tcaaaatacg gaatcatcaa cccaggggaa
     7981 gtactggaag atgggttata caatgtcaaa aactttgttg aaaaaccaga tccaagcaaa
     8041 gcaccaagtg atttagctat tattgggcgt tacttattga ctccagaaat ctttgatgta
     8101 ttatcagaac aacaaccagg tgctggaaat gagatccaac taactgacgc aatcgatacg
     8161 ttgaataaaa cacaacgcgt atttgcccgt aaattcacag gtaaacgtta tgacgttggt
     8221 gacaaatttg gttttatgaa aacaagtatt gaatatggtc tgacacatcc agaagtaggg
     8281 gcacctttgc gtgaatacat catgtcttta ggtaaaacat tagaagcaga aggaagcaag
     8341 atcaatcata atccaaaagg aataacagaa atcaaagaag aaaagaaaaa ataatctttt
     8401 tttctaacga gcttcagctg ctaattgaat aaaaagtagt tgaagctcgt ttttatttgt
     8461 ttaaaaaaca cgaaaatgct atccttatag aaaagagaga caagaaggga gtttgatcat
     8521 gtcaggagga gaaattgcag gattgatcgc agcaatcgcc tttgcgatac ttgtcgtttt
     8581 catcgttcgc gtattgttgc aagtttccaa aactttagaa aaagtagacc agactgtcgc
     8641 agaagcaaat acgacaattg agatcgtcac acaagacgta gatcttttat ctagacaagt
     8701 agaaggcttg ctagtcaaaa gtaatgagct gctcttggat atcaatggaa aagtcgcaac
     8761 gatcgatcca ttgttcacag cagttgctga tttaagtgaa agtgtatcgg aacttaatca
     8821 ttctagtaaa aacatcgtga cgaaagtcgg cggaatcgga aaaacgacag ctaaagcgac
     8881 tgttgccgga aaagtcggtg gaacagcgat gaaatttttt aaaaacaaaa aacatactga
     8941 aacgacagga ggaaaataag aatggctaaa aaaggtggat ttttattagg tgcagtagtt
     9001 ggaggtacag ccgcagcaat cgccgcatta ttgttagcac ccaaatctgg taaagaactt
     9061 cgggatgaat tggctgctca agcagatgat ttgatggaca tggcttcaga ttacacagat
     9121 atggcaaaag aaaaatcatc tgagttaaca ggtattgcaa aagaaaaagc gcaaggtctt
     9181 tctcaacaag caggggatct agcaggcaat gttaagcaga aggtaagtga atcagcagag
     9241 gaagcaggaa gtgaatcaga tgatatcttg ttaaacctca aagaacaatc agctgattta
     9301 tctaatcgtt tcaaaaaaac agcagaagat ctgaaaaagc cagcagacga attaggtagt
     9361 attgctaaag atacttcaga agatatcatg atcgatgtaa aagatgctgc agataaagca
     9421 ggagagacgg tcaatgaagg aatgtcagaa gcaaaaccag ttgcagatga cacaaaagaa
     9481 atgttgaaaa tggcaaaaga agatgcaaaa gaagctaaaa aagaaatgaa agatacaatg
     9541 acagattcga ctaccaatca ctaagattac tcatttctag aataaaaaag gtgagagcgg
     9601 accagctctc acctttttta ttttcattac tctaaaacaa gtaattcttt tggtgtagtc
     9661 gtgaatggca cacaaccatc acttgtcaca tacacacaat cttcgatacg tacccctact
     9721 ttttcaggaa tatagattcc tggttcaaga gagaagcaca tcccttcttc aatcactaag
     9781 tcgtttcctt ctacaagaga agggaattcg tggacggttg ttccgattcc gtgacctaaa
     9841 cggtgattga aatattcgcc gtagccatag ctgttgatca cgtttcgtgc gatttgatca
     9901 agttcacctg ctgtcacacc tgggcgaact gcctcttgtg cggcaagctg tgcttcaaga
     9961 gtgatgttat agattttttc ttggaactca gttggttttt gataagcaac tgtacgagta
    10021 gcatcactgc aatagccatt ccaaataacg ccaagatcga ataacactag ttcatttggt
    10081 gaaaccttcg tgcttcctgg tgtaccatga gggcttgcag catttgctcc tgcaaggact
    10141 aatgtgtcga aagacatttg tgaaacacct tgttttttta attggtattc gatttctgcg
    10201 ataattgctt gttcagttac gccttctttt actgttttga agccaatttc aaaggcaaca
    10261 tcggcccatt taccagcttc gatcagtttg tctttttctt ctaccgtttt gatcagttgc
    10321 agacgctgga taatagaagt tagatcggtg taagaatctg tacctgtcaa gatatcagag
    10381 atagcatcga agcgatcgac agtcagagca ttcttttcaa tacctacatt accaggttgc
    10441 ccatagcgtg cgcgaagctc ttttccgatg atctcccaag gattttcgct gtctaagtag
    10501 ccacgtacat cgtacgccca gctgctgttt tgagcatctt caacttccag agcaggagtg
    10561 aaaaggaaag gatcttgttc cagagggata aaaagagcta gtacacgctc gtgaggatca
    10621 ctttcatatc cggaaaagta agcgatgtgg ccagggtcgc tgatataagc caattctact
    10681 tggtgttgtg ccatccattg acgtagttca ttgattttta cgttattcat ttggcaacct
    10741 actttctaaa ttaattttgt attcaataat tgtaccactg attgattaaa agaccaacgt
    10801 tcttaaaact gaaaaaagta aaaattttca tgaaaacaga gataaaacgg ttgcaattgt
    10861 tgagattatt tgatatccta ttttagaaat gtaagacgta ttttcagaaa acatataaaa
    10921 tatgaatagg agaacacaaa tggaaaagca aacaatcact atctatgatg tagcgagaga
    10981 ggcaaatgtt tcgatggcta ctgtttcacg tgtcgtaaat ggcaatccaa acgtcaaacc
    11041 agcaacaaga aaaaaagtct tggaagttat cgatcgcttg gattatcgtc caaatgcagt
    11101 cgcacgtggc ttggcaagta agaaaacaac aacagttggg gtaatcattc ctgatgtcag
    11161 caatatgttc tttgcctctt tagcacgtgg tatcgacgat gtggcaacaa tgtacaaata
    11221 caatatcatc ttagcaaact cagatggcaa cgatcaaaaa gaagtcaatg tattgaataa
    11281 tttattggct aagcaagtgg acggcgtgat ctttatgggt caccatatca cagacgaaat
    11341 ccgtggagaa ttttcacgtt caaaaacgcc tgttgtacta gctggttcca ttgatccaga
    11401 tgaacaagta ggtagcgtaa acattgatta tgtgtcagca acaaaagatg cagtaaataa
    11461 attagcaaaa agcggcaata aaaaaatcgc ttttgtcagc ggtgcgctga ttgatcctat
    11521 caatggtcaa aaccgtctaa aaggctataa agaagcatta agcgaaaaca atctaccata
    11581 tagcgaagga ttgatttttg aggcacaata caactttaaa gatggtctgt ctctagttga
    11641 ccggatccat aatagcggtg caacagctgt ctatgtttca gacgatgaat tagccattgg
    11701 tatcttggat ggtttattag accgcggcgt aaaagtacca gaagaattcg aaatcatcac
    11761 aagcaacaat tctttactga cagaagtagc tcgtcctcgt ttgacaagta tcacacaacc
    11821 attatacgat ttaggagcgg tatccatgcg cctattgacg aaaatgatga ataaagaaga
    11881 agtcgaagaa aaaacgatta tcttgcctta tggaatcgaa gaaaaaggat caactaaata
    11941 ataaaagatc aatcatttaa aggttgttcc aaaagtggtc aacttctgga acaacgttta
    12001 tacgagaaag ggggttatga caaaattttg tcataacccc tttttttttg tgactttaaa
    12061 aaaagagaaa atggcggaaa caaaccttac gtccatcgta tacgacacaa tctgtgcgta
    12121 gatgctacat tttgttcact actatcaaaa ttactttaaa atgaaaacgt gataaaaaag
    12181 aaagtagaaa agtagataag taactctaaa aaatccaaaa aaatccaggc tcgattgcgt
    12241 acttatctta aaggagaaac aatccatcat atataacccg aaaggagact tctatttgcc
    12301 taagaaaaga cgaaagacca tagtcatcat tttgaccatt cttactatag gaatcggagg
    12361 gaaattttac atggacaaaa gagaaatgca aagacagaaa gatttactcg cagtagaaaa
    12421 acagtccgtc aaagttctga aaaatacctt tgcggatatc aaggaagtga agattgaaaa
    12481 atctgctcga aatgaaatga cgggttcata ccggatagtt attctaatga ctaataaaca
    12541 agatcaatcg atttattttt cttatagttt ttggaaagaa agaaatgaga taggaagtta
    12601 cggaatagtt gatgaaaaaa agcaaaaaga aggaaatacg ctcaataaag ttaaagtaac
    12661 ttactctaat ggaaatgagg aaagtattta atgattactg acaaacaata tagtgaatta
    12721 agtgatgctg tttactggct agatcctaaa catagagact atgtacctga aatgcaagaa
    12781 aatttaagtt ttaaaattaa cggcactatg tataaaatcc taaaaatcaa aaactccttc
    12841 gacggcatgc aggcgatggc tgtagcgcca atcgtccata gcaaattgga gaaaaatttc
    12901 aaaaataaaa aaattcctgc aaactttcgc gtacttaaat aatgaaggtg tcaatactta
    12961 cataaaaaga aaggaggcac gttcttgtca aggaaaataa gaaaactcag cttagccatt
    13021 ttattaatcg tcatattagg gatcggaggg aaagtctata tggacaaaag agaagcacaa
    13081 aggcagaaag atttactcgc agtagaaaaa cagtcagtca aagttctgaa aaataccttt
    13141 gcggatatca gggaagtgaa ggtagaagaa tttaaaaaaa accctgttac cggaagttat
    13201 ggtgcacttg tgacaatgac aaataatgaa gggaaatctg tttattttga ttatagtttt
    13261 tggaaggaga gaaatgaact tggtggtttt ggcattgaga acagagcggt acaaaagaca
    13321 ggagtcacta taaaaagagt aaaagtaatt ttttctaacg gacaggagga gttagtatga
    13381 caataaacga taagcattac aatgatatta gtgagaaggt ttattggcta gatcccaaat
    13441 atccccgcta caatgaggga tacaaaaaga actctgttaa agaatttgct ggtatggaat
    13501 ttcaaatcct acaaatcaaa gactcattgg acggcatgca ggcgatgtct gtagcgccaa
    13561 tcaagaacgg taaagtggat ctttctcaag tagtgattgc ttttgcagga acgaacttaa
    13621 gtgattggaa agatttggaa acggatgcaa gaagtatcat gaatctctac gggaatagac
    13681 caggagcaag tttttctgaa aaagcaacaa caaacaagat tttatccgtt agtggattga
    13741 aaacagcggg gcaaatcaat tcagcgattg catttgctaa tgaagtaaaa aaggaatatg
    13801 gagaagctgc tatttctact accggccatt cattaggtgg attcttagca ttatatgtag
    13861 cagcagagaa acaatggaaa aatgtcggct tcaacgggcc ggacccgtat gatctcttat
    13921 caccagaagc aaaaaaatgg gtggaagaaa atcccggtat gctcaccaat taccgaaatc
    13981 acgccgattt tatcggtaat tttggtggta atggcacagg tgctgaagtt cggatcagta
    14041 tggaaacagc attcttgaat ctgcttgctt cccacagttt aacctcttgg aagtttgatg
    14101 aaaatggaaa gctgattatt cctgaaaatg aaaacaatgt taaagctcga aaacaacaga
    14161 aagaaaacca agcgacccag ttgttttcct atcagttgca agaactgaac aacatacgta
    14221 aaaaactacg attaagtggc ggccggctga cagttaatga acaaatttac ttagatgaca
    14281 cacaagctag gttagtagtg gaaaagttat cggcaaaaat gaaaagcgct atggaagaag
    14341 ttatccgcat caatcaaaga ggaatttttg agctagagaa aaaatggcaa gaaggcttgc
    14401 ggcaagcacg tgttgtagcc ccttcgttga ctgaacagga aatcatagaa gcattagcta
    14461 gtgtgggcgc cacaaaagaa aacttagtcg attctgcgaa agaagtctat ctgctacgaa
    14521 tcaataaggc aagacgtatg ggggaggcct ttgatacatt ggcgaaggag atccaagaca
    14581 aaatcaatga aatcgtggca cgagatcgag aattagcaca gcaattcaat taaaggagaa
    14641 gtagatgaaa ctagaagaat tggaaagaga ttatcgaagt gaacgaagac aattagaagc
    14701 tcaagaagat gaattgatcc actttaagaa tcaaggatta cgattgatgg agcaagagga
    14761 gcagcgactg catcatgtat taaagaactt agctttagat aatgagcctc tgtttcaagc
    14821 aagacgaagt tattcgaatt atcaggagca ttatcaacaa gccctacaag tagaacgacg
    14881 aaaactctat cagcagcaag atgaactaga acgttcctat cgtcaaaaaa tcagacaaat
    14941 aactatggag aaatcatctt atgaatgaat ttttaatgca attactaaaa gaattagaga
    15001 aaaatctaca tcgatcaaat gtacgttcag ttggtatggg gatatctcag caaatttggg
    15061 cagaaaaacg attgacagat caaaactacc aaatgactgc agaagaagct gctcaagaat
    15121 tttctaaccg acatgtacac ttgaaaggat cagtaaaagg gcaatttgca gaaaaactaa
    15181 ctgaattatt ccaaagacaa gaaaagaaat taaaacagtt tcgttagtaa agttatatag
    15241 aaaaatttt
//
LOCUS       DAZKOB010000063        15072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000063 DAZKOB010000000
VERSION     DAZKOB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.063"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG8M6_001897"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG8M6_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG8M6_001898"
     CDS             706..1734
                     /locus_tag="ACG8M6_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0457563.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG8M6_001899"
     CDS             1731..2489
                     /locus_tag="ACG8M6_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0457564.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG8M6_001900"
     CDS             complement(2575..3315)
                     /locus_tag="ACG8M6_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0457565.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG8M6_001901"
     CDS             3376..4629
                     /locus_tag="ACG8M6_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0457566.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG8M6_001902"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG8M6_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG8M6_001903"
     CDS             5300..5626
                     /locus_tag="ACG8M6_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0457567.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG8M6_001904"
     CDS             5694..6407
                     /locus_tag="ACG8M6_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0457568.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG8M6_001905"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG8M6_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG8M6_001906"
     CDS             7690..9555
                     /locus_tag="ACG8M6_001906"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0457569.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG8M6_001907"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG8M6_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG8M6_001908"
     CDS             11120..11821
                     /locus_tag="ACG8M6_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0457570.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG8M6_001909"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG8M6_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACG8M6_001910"
     CDS             complement(12857..13066)
                     /locus_tag="ACG8M6_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0457571.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13307..13882
                     /locus_tag="ACG8M6_001911"
     CDS             13307..13882
                     /locus_tag="ACG8M6_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0457572.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="ACG8M6_001912"
     CDS             13882..14322
                     /locus_tag="ACG8M6_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0457573.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="ACG8M6_001913"
     CDS             14338..15039
                     /locus_tag="ACG8M6_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0457574.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5083 a         2501 c         3179 g         4309 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaaa tt
//
LOCUS       DAZKOB010000064        14855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000064 DAZKOB010000000
VERSION     DAZKOB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.064"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..739)
                     /locus_tag="ACG8M6_001914"
     CDS             complement(26..739)
                     /locus_tag="ACG8M6_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0457575.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            914..2338
                     /locus_tag="ACG8M6_001915"
     CDS             914..2338
                     /locus_tag="ACG8M6_001915"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0457576.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2637..3344
                     /locus_tag="ACG8M6_001916"
     CDS             2637..3344
                     /locus_tag="ACG8M6_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0457577.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3331..3654
                     /locus_tag="ACG8M6_001917"
     CDS             3331..3654
                     /locus_tag="ACG8M6_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0457578.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3743..4780)
                     /locus_tag="ACG8M6_001918"
     CDS             complement(3743..4780)
                     /locus_tag="ACG8M6_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0457579.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5101..6084)
                     /locus_tag="ACG8M6_001919"
     CDS             complement(5101..6084)
                     /locus_tag="ACG8M6_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0457580.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6499..7269)
                     /locus_tag="ACG8M6_001920"
     CDS             complement(6499..7269)
                     /locus_tag="ACG8M6_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0457581.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7262..8197)
                     /locus_tag="ACG8M6_001921"
     CDS             complement(7262..8197)
                     /locus_tag="ACG8M6_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457582.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8513..10162)
                     /locus_tag="ACG8M6_001922"
     CDS             complement(8513..10162)
                     /locus_tag="ACG8M6_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0457583.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10279..12882)
                     /locus_tag="ACG8M6_001923"
     CDS             complement(10279..12882)
                     /locus_tag="ACG8M6_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0457584.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12894..13943)
                     /locus_tag="ACG8M6_001924"
     CDS             complement(12894..13943)
                     /locus_tag="ACG8M6_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0457585.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13969..14283)
                     /locus_tag="ACG8M6_001925"
     CDS             complement(13969..14283)
                     /locus_tag="ACG8M6_001925"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0457586.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14299..14745)
                     /locus_tag="ACG8M6_001926"
     CDS             complement(14299..14745)
                     /locus_tag="ACG8M6_001926"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0457587.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         4401 a         3076 c         2498 g         4880 t
ORIGIN      
        1 ctctgaaaaa ataattttcc cgagcttatt tttcaacaaa aacagcttca aaaacaaagt
       61 cttgatataa atgtctcgat accgaatact cgaaaggttt accagacgat agagcaacaa
      121 ctttgtctat ctcaatcata aattcacctg gatctatttt taatattttt gcgtcaaatt
      181 cattcgcagt atctcctttg atacgtatgt gcgaagtctt tatttccaga cccagttcat
      241 ttgtgatata agcatataca gatttcttta aatgttttgg ttccaagcct ggtatgactg
      301 ctaatggcat gaatgtttgc tcaatcgagt atggttgaca atcaataaaa cggcttcgta
      361 caatattata tacatagtct tttacactga tacttaattt atcagctact tttttcgatg
      421 gaagttcaat tgagaaagta tggatttctg aatctacgtg ttcttcacca accacgctaa
      481 acaaaccttc gtttggacca agaactcgtg agttatcaat tggtgttctg acgacttcag
      541 tcccataccc acttctactt cgcaaaactc cttcggaaac aagtaagtct acgcctttct
      601 ttactgttaa cttgcttgta tgataaataa ctgccaattc atctcctgta ggaagtttct
      661 caccaatttt atactctcca tttacaattt ttttctttat atctaagtaa atttccatat
      721 acttcgcaat ttttttcaat ggtgaccctc caatcaaaaa catatcttca tctatacttt
      781 aacataaata aaccgataaa atccacatca aaattataca cacaataaaa aagatatata
      841 tatttaaaca aaactattta ctttaagttg taagcggtgt tatcattaag tggaaacagg
      901 aggtaaaaaa attatgacaa ctatgaaaaa gctaactttt ccagatgatt tttggtgggg
      961 ttccgcttgg tcagctgagc aagcagaagg aaaaggagaa acaggaaaag cagagactat
     1021 ctgggatcgc tggtttaaag aacaacctta ccggttttat caacgaatcg attcctctat
     1081 tacaaccgat cacatccatc aatataaaga agatatagaa ctaatgaaaa aaactgggca
     1141 taactccttt cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg gtaccggaaa
     1201 agtaaatccc aaagccattt ctttctatcg aaaattattt tccgaaatga ataataacgg
     1261 aatcaaagta tttgccaact tatatcattt tgacatgcct gctactcttc aagataaagg
     1321 aggatgggaa tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa cttgttttaa
     1381 agaatttggt gacttagttt gtcattggtt taccttcaat gaaccacttg ggcctatttt
     1441 aggtacttat cttgaagatt ttcattatcc aaacctcatt gactttaaac gcggagcgca
     1501 agcagcattc ttcactatac ttgcacacgc aaaagcaata caagtattta aagaactagc
     1561 tcttcctagt aaaataggcg ttattctaaa tttgagtcct acttttcctc gtagtcaaaa
     1621 tcctgctgat gtgaaagcag cagaaatcgc tgatttattt tatacgcgga gctttttaga
     1681 tcctatggtt aaagggaatt tttctaaaaa gcttatcctg ttattacaag aatataatca
     1741 gcttcctgaa gattatacga aagaagatct tgtatgtatc tacgaaaata ccgctcaaat
     1801 tcttggacta aactattacg agccacgtcg cgtcaaatct cgttcttcta tggtggatac
     1861 atctagtcca tttttaccag agtggttttt cgaaccacat atcatgcctg gacgacgaat
     1921 gaacgaatac cggggatggg aaatttacga aaaaggaatt tatgatttgt gcatggatat
     1981 caagaaccat tatggaaaca tcgaagcatt tatctcggag aacggaatgg gcgttgcgga
     2041 tgaagaacgt ttcatggatg atacagggca aataattgat gattatcgga ttgagtatat
     2101 caaagatcat cttgcttact tatggaaggc aataaatgaa ggatgtaaca ttaaggggta
     2161 tcatctttgg acatttattg attgctggtc atggatcaac gcctataaaa accgatatgg
     2221 gctggtttca ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg gcgaatttta
     2281 caagcaatta agtgatacaa atggatttag atacgatgta gacttgttag ttgaataata
     2341 ttctaaaatt ccacttgaag tgaacacatt agaaaacaag aataatgaga aataccaata
     2401 acaaatactt tttcttcata cttcatttat acttgtcagt ataagagact gtgacaaatg
     2461 tagtgatcct cagaagttag gtctggaaat ctaacttgag aagatttttt taaactacgt
     2521 ccagtctttt ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa atttagaata
     2581 ttcacaagtt tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt ttgttattgg
     2641 atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt tttggttata
     2701 tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cgggtttcat ccgctggtcg
     2761 tcacgttgat gtccctgctg atctatgctg gttctgccca atttatcacg gtcagcatgc
     2821 ttgctagcca tagtccactg ctctccatcg ttctctcgac ctttctagtc aattcccgaa
     2881 tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg cttcaaaatc
     2941 tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat aaattgaact
     3001 atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt tcttatttga
     3061 cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact gatccgaaaa
     3121 agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat cttcaagtca
     3181 tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc actttgattc
     3241 tggtatatgt cggactgatt tttatcccaa gtaacttagt gattttagtt gtaacactga
     3301 ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac attgctgact
     3361 attttaggtt gtactttagc aacttggctt tctaggatcc taccatttgt cttactgaaa
     3421 aaattcgatt tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat cgtcattatg
     3481 tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc gcagatcaat
     3541 ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc aaaaagctta
     3601 ttggtgatcg tcttggtagg aattctttcc ctttcgttga ttcgcttatt ataaataaaa
     3661 aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa gtcaaataag
     3721 aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg acttttccta
     3781 atatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca ttcatcccat
     3841 ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa gcagcaatgt
     3901 gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta gcgaacatat
     3961 actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac ggctctttga
     4021 tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt tgatcaaagt
     4081 agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct aattcacgaa
     4141 aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt tttttcagcc
     4201 attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa tgcgctagtt
     4261 gattcaaaat cgaaccgacg cccccagctc cgttgatcac taagattttt ttcccgcggt
     4321 tggcattttc ttctggagtc agaccaaatt tttcaaataa caattcataa gcggtcaatg
     4381 aggtcaacgg caaggctgcg gcttcttcat ctgaaagatt ttttggtgca agagcgacaa
     4441 tacgttcgtc aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt gtacctgcgt
     4501 aaaaaacgcg atcgccgaca gaaaattttt gcacttcatc acctacagca acaactttcc
     4561 cgactccatc aaaccctaag atacgtaaag catttcttat cccgttatct tggcgaagtt
     4621 tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc cgtgaactgg
     4681 gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc gacaaccctt
     4741 cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct ccttagcttc
     4801 atcaagttgg agcatccata cagttttcat ttcaatatat atagtatgct atttgttcca
     4861 cttttattca tcataattat tctcttactt aaagtaaact atttgcactt ttttggttaa
     4921 aaaagaggtt gcgacaagac ttttgtcaca acctctttta aacggtgttc aatcaactga
     4981 ccctcggtat tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt tggctgttct
     5041 tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc aagcaagaaa
     5101 tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga tctctcgtac
     5161 ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc ctggaaatcc
     5221 tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga tgatttctga
     5281 ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt tttctgtatg
     5341 ttccctcggt tctttttcaa atgcataagg attcttgaaa atcccccggc caatcatgat
     5401 gccatctacg ccatattttt cggccaattc ttctcctgtc tggcgatctg ggatatctcc
     5461 gttgatcgtg atcagtgttt gcggagcgat ttggtcacgt aaagcaatga ttttagggat
     5521 gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat ggacagaaag
     5581 attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag acatttccgt
     5641 atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagccg cttcaatcaa
     5701 ttgtgccgct acttctgggc gaaggatcaa gccactgcct tttccacgtt ctgctacatt
     5761 agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg ccatagcaat
     5821 gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca tcggctgttc
     5881 atcttctgta aaggtcaatc ttcctcgtac actgtctttt ccatctggat gacagaaact
     5941 gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct ttacgacatg
     6001 gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag gctttggcag
     6061 ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa gcagtgaagt
     6121 cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa gcaaagtgaa
     6181 cagcttaaaa gatgtttctg attgagcaaa aaaagactgt gacaaaaatc ttatcacaat
     6241 tccttttccg gaataaatgg tgttcaaaag cttgcatctg cggtaataag tccaaccaaa
     6301 cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt
     6361 tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt agctcaacaa
     6421 acgacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg tcaaaacgct
     6481 gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca aataccaagt
     6541 aacggcgtac aaaacaacga tgaatagcac aagtacgatc atcgatgttg ctactggcac
     6601 atcgattgtt ccaaggaatc cgtaacggaa accggagatc atataaacga tcggattgac
     6661 tttcgaaaca gcttgccaaa atggcggcaa catagagatg gcatagaaaa cgccacctag
     6721 ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcgtcat aagactgagc
     6781 aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt gccgtcatca ataatgtaat
     6841 gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa tgatcgttac
     6901 gagagagccg acgagaatac tgcgacccac tccgccaatc acaaagcccc aaatgatcac
     6961 atgcgtagga acaggcgcta ccaagatttc ttcgatgttt ttctggaatt tttgagagaa
     7021 gaaggacgaa gaaacgttgg catacgaact agtgatagca gacatcataa tcaaaccagg
     7081 cacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgaccac cgatcatttt
     7141 tccgaaaata acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg tttggaccca
     7201 gatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag taaaataaag
     7261 attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaaa aacagttctt
     7321 ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt gtcagctgat
     7381 cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc tgttcatctt
     7441 caaatgtaca agcgtatcct tcaatcacag gtttggtctc atatggcgct aaatcaaaga
     7501 taaatgtttc aaattgcagt ttggatagaa gctctttcat actagtattc tcgatcagtt
     7561 cacctgattg gatgatcccg atgttgcgac aaagcatttc agcttcttct aaataatgcg
     7621 tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg gagaaactcc cacatctcgc
     7681 gtctcaattc aatatcgact cctgctgttg gttcatccaa gatcagcaaa cgtggttcat
     7741 gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc atacgtgcac
     7801 ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg cgtttcatcg
     7861 cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc tggaccgttt
     7921 caaagggatt gaaattgaac tcttgcggga ctaatccaat ctgttgcttt gctcggacta
     7981 gatccgtatc cagatcataa ccgaagacat tcacttttcc agatgtttta ttgactaaag
     8041 aagtaatgat tccgatcgtt gtagacttcc cagctccatt tggtcctaat agcgcataga
     8101 aatctccttc tttcacggaa agatcgattc ctcgcaatgc ttcgacccca cttccataaa
     8161 cctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct ccttatagac
     8221 atgattcttt attgtatcat ggaatacagc agatgaaaaa acaacttggt tccagcgaaa
     8281 aatcaggttg ctgggctcag gaaaatctcc taatgaaaga catcaatcgg acgaagtcag
     8341 acaacctttt cagaaaaaac aaatcgaaga taaaggatta ctattcatat gtgcaacaaa
     8401 aggagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
     8461 cggaagcaaa agccaagatt tttgatccaa taaatacttt cgatcacttc gcctatccca
     8521 atacatgcca ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg gtttctacta
     8581 aagtctctgg gtaatccaaa actttcagca aggcaatcgc atttcgggtc cttgaaggtc
     8641 cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt tcttcaaagt
     8701 ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa
     8761 atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa gcaatccgtt
     8821 cgatcgtatt tgtccctttt aaaatctcat ctacgaagca gtagcagcgt tctttggaag
     8881 ccacttggtc caacaaacgt ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt
     8941 caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc agggtaaact
     9001 gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct atacttttga
     9061 catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc tgccagttga
     9121 ccggattcac taccgcttct tttaacaatg gatgatagat atctttcgct gtcacaccgc
     9181 cctcagtaaa ctctggtcgg caagtgatgg gcatataagt acggtaattc aatattgcag
     9241 cggctacttc taattctccc agcaattccc acagcttgat ggcttcagtt gaatgctttt
     9301 ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg atgttgacat
     9361 attcaaaaat caactctgct tcactcccac tcttcatacg aaatgaaaaa cctaatttag
     9421 ggatgtctct taatggttta gtttgctgtt tcagctcttc ttgaagtggt gtcggcagtt
     9481 ttgctagctg attggcggct gaaatcgttg aaaccaaata actcatgcta ttcagctctg
     9541 tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaac ccaccgattg
     9601 ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc caaacccacc agcggaagca
     9661 agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca tccgctagat
     9721 attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat tggatttttt
     9781 ctctcagttg aggattttcc tgataaaaag caatcagttt ttctaactgc tgatcttcac
     9841 caaaatcaaa gctgcgtaaa cgttgataca acgcttctga acctacacta gaataggtac
     9901 tgttgattcc ttcgaatact tcgaacatat ccaggtcata ccaagtaaga tcatcgattt
     9961 cgctatccca tgaacggaac ttcttctccg tgagccatgc ttccttcaag ctttcttctt
    10021 tatcaaatct cggttgataa ggaatttttt cccatttgct acgaactgtt tcctttaatt
    10081 tcaaacgtga aaaaatgtct aaagcaacta ataaaataac agctgcaata ataattaaaa
    10141 cgataaattc tctttgaccc atatccctct ccgttccttt atttgttagc tttagtatag
    10201 cactgttgcg ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa aggtgttttt
    10261 ttcttgtata ttacatattt atctctcatt tatcaaaatt gacaaataat catagggagg
    10321 aattgttgtt aattcttgtt tattctccag tgcattgaca actgtcccaa gggaaaggat
    10381 agcctcaggt actatttttt cttgttctgt tggattagct aagcgaagaa caaatccttg
    10441 ttcatgaaaa taagaaggat aaatcgatga tacaattaag tcttcaggta attcaatcaa
    10501 tgaaaattta cgaggaacat caagttcatt ttgtaatggc caaatcttgt tatctaacct
    10561 gtgaataaat acattcagtt tctgcttttg ataagaaata gattgttcta accgtttttc
    10621 tgcctgtttt gccactttga actcattaaa tcttccttct tctacattaa atccaaaagt
    10681 gactttatac tcacctatct cttgagctag cggtgtttcc atcatgacat gcccttcatt
    10741 tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc caagttcccc
    10801 tgttgttgaa aataaggtta tgattaattg attttctata cgttcatact cttttaaacc
    10861 atctgcataa aacgtaaaat aatgatcatt ttctgccaca gagacactct tatcgaatat
    10921 ttctaaatta acaggtttct ctaccatagt ttctgaccaa ttcggaggaa tgacatcagg
    10981 ggtattttca ataaatccgt tttgaatttg tgcaattgat ttttcagatt tatctaaaac
    11041 attaattgct aagcgcatac gatgcgagta aacagtatta tgaattttga gtgtgccttc
    11101 aacgatttga ctttctccca gactgaacga tatttcataa gatatttctt ccatttcggg
    11161 tttttcagat aaacgatctt ttaaatcttt aggaagttga gcttttccgt aaacaactaa
    11221 tgtttcttgt aaagaatctt tatatacttt agctgtttct aattttagag atagttctgt
    11281 atctccttct aatggtgaat aatcatacgt atcgccatca tttgctgaat cgatcaaatg
    11341 aacgaaatca agatattgtc tatctccaac tatcaaattc aattgcccat ctttaaaaga
    11401 aagtttatat ttattgttac ttatctcagt tttgtctatc tcgattgctg tttctaattt
    11461 ttcattattt ttctcaaatt caacaacagt gtatcctagt gctggcacat ttacatttat
    11521 ttttatgtca agttcataat aagctggttc atcaaaatat tcgaaacctg tagcaatcat
    11581 ccgataagca tttttccgtt ctggaaaata aacttctttt tcaatgatat tatagggact
    11641 acctttgaag tgtattttct ttgaatcagc tactacatgc acgattttcg atccactata
    11701 attctcagtc gttgtgttga aaacaaggac ttcattcgat tttagcttta atgcatcagc
    11761 catccgtttt acaataaggt tttcgattcc gtcagctatc tcattagctt ctttgattcg
    11821 atggaaaata tcttccgcaa cattatctga aacacagcca ccaatactat catgagcctg
    11881 gttttgcaaa atctttttcc ataaccgacg aagtaggcct ttactgatat ggataccatt
    11941 tctttctgca atcagcatga gcggctcaac tcgttttaga attttttgtt ccaatttaaa
    12001 attttctatt ttcatttttg ttcttactga gccaatagaa cgatgaacac gagagtaaac
    12061 cggctgacga agttcacctt tatattcagg taattctttt ttgtttctga taacttctac
    12121 ataagaagga taatctgaaa taatattgtg gaatttactg tatttattca acttctctac
    12181 gacttgatca aaatttaaaa tcattgactt ttgatctaca cccgaaggta ctaaaatatc
    12241 tttgtttttt ccttgcatat gaagcatttc aactgcttca tccaactttt cttttgcaaa
    12301 ttcaggaata tctttaattg cgtctaaggt catcattcca gtcatataac caaatggaaa
    12361 attcatggca taaacaaatt tatctcccaa tcctttccag ataaaataag gagatggaac
    12421 catcgtttca aagtttaatc ctcgccaaca catgaaatta tcgatttcta cttgatgcaa
    12481 aagtgtaggc aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta ccatcattgg
    12541 atctccatat ttaaggatcg tatcactgat tccaatcatt aagtttctta aaattgattc
    12601 agcatccact aataaggcat ctgtttgtgc ataccacggt cccacgaata gttgtcctgt
    12661 tttcatcagt cgttggataa ccttaagctt ttgcggattg atctccaaat actcatcgac
    12721 gattgaagtt tgtccatcta aacaaaagtt tactttaggg ttcctttcca attcatctag
    12781 aacttcagta aagacctgat cagacaatac gatggcatct tcatttgtaa aataccattc
    12841 tctgtcccag tgcgtgtgtt gaattatatg tgcatttacc atatcagatt ctcctacttt
    12901 ccttcttcta ctgaatttac ttgttgttct tttttatact tgatggattt tagtgcattg
    12961 accattaacg ctgtaaccac tcccccaata acaattgata ctaaatataa aggtatattc
    13021 gtaacaaaag gaattgccat aaaggttgaa agcattgttt cttgagtaac accaaaaaat
    13081 ccatttagac cacatgctac tgcagtgccc accacgatgc ttggtattac tcgtaaagga
    13141 tctttcacgg caaacgggat cgtcccttca gtaatcccca caaggcccat aatcatagct
    13201 gtctttgcat tatctcgttc cgattcatca taaagttttt tgtttaaaat tgttgctaaa
    13261 aacattccaa atggcggaat agcaattcct aataacatgg ctgtactagg tccaaagctt
    13321 ccgcttgaaa aagaagcaac cccaaaagca tatgcgattt tattgatagg tcctcccata
    13381 tcaaacgcca tcattcctcc taaaatagcc gacatcaaag cagcatttgc tcctgacatg
    13441 ccttctagcc aattagtcag tcctgttgtc attgcttgaa taggcgtagc tagtacatac
    13501 cataatatga ttccaacaac taatgtagta acaaaaggaa tcagcataat tgattttaga
    13561 ctaataactt gcatcggtac ttttactttt ttcaatgcat tagctaaata gccacaagct
    13621 aatccagcaa ccattcctcc aataaatccc ataccaagat tttgacacat catcccagtg
    13681 ataaaagctg gtgctagacc gggacggtca gcaattgaat atgcgatata ggctgaaatc
    13741 atcggtacga taaactgtaa tcccgtctga ccaatcattc gcaggactcc ccagaatgta
    13801 tccgtctggt cccaaacacc tgtgccacca aaaatgactg caatagaagt tagtaaccct
    13861 gctgctacga caagcgggat catataggaa accccacttt ggaaatgatt tagaatttct
    13921 cttccttgtt tcttgaattc cattagtaac tcccccacga tatatttttt atttgttaat
    13981 taatgcaatt gcttcatcaa tcgtctcttc accatctttt actacctctg aggtccctac
    14041 tgttaaaatc ggtttaccgt caaagcgacc tcgttctgaa acatttgtat ctactgcaaa
    14101 tatgacaaca tctgcctcgg aaacatccga aattgaaagt ttgttttcta tccccatagc
    14161 cccctgtgtt tctactttaa tatctaaccc tttcttcttc gcaaactttt ctaaattagc
    14221 ttttgccata taagtatggg caatccctgc gatacatgct gttactgcta ctatttttgt
    14281 catatgaaat tcctcctttt aaaatgatag agcttttacc aattcttttt tatttctcga
    14341 atcttttatt cttttaacaa actcgtcatc catcaatctt ccagataatt ctgctaaaat
    14401 acgtaaatga ttagtacctt tatcagtgtc ttttattgct aacaaaaaga cgagatcaac
    14461 tggttgatca tctaatgcgc cccattcaac agaccgcttt aacttggcta cagccactgt
    14521 tgattcaatt actgctaggc ttttgccatg aggaatggcg atatttttcc caattcctgt
    14581 ggtcatatgg ttttctctgt caaatacgtc tttttgaaat tgtttgtggt cagacacaaa
    14641 cccattagtc tctaacaatt tcgataattt ttcgactaca ctttttttat cagatgcatt
    14701 taaatctatc actatattat ttatattgat tttttcactg atcatttata agccttcttt
    14761 caatatataa actaattttt caaacgacga actcgttttt aaatttgcta ataactcatg
    14821 attcgaagcg atttcattga atgtttcata aattt
//
LOCUS       DAZKOB010000065        14625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000065 DAZKOB010000000
VERSION     DAZKOB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.065"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG8M6_001927"
     CDS             4..816
                     /locus_tag="ACG8M6_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0457588.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG8M6_001928"
     CDS             813..1613
                     /locus_tag="ACG8M6_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0457589.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8M6_001929"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG8M6_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0457590.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG8M6_001930"
     CDS             3110..4819
                     /locus_tag="ACG8M6_001930"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0457591.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACG8M6_001931"
     CDS             5031..9389
                     /locus_tag="ACG8M6_001931"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0457592.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8M6_001932"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACG8M6_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0457593.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8M6_001933"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACG8M6_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0457594.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACG8M6_001934"
     CDS             11227..11520
                     /locus_tag="ACG8M6_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0457595.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACG8M6_001935"
     CDS             11517..11828
                     /locus_tag="ACG8M6_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0457596.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACG8M6_001936"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACG8M6_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0457597.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8M6_001937"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG8M6_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0457598.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4983 a         2598 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DAZKOB010000066        13978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000066 DAZKOB010000000
VERSION     DAZKOB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.066"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACG8M6_001938"
     CDS             140..460
                     /locus_tag="ACG8M6_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457599.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACG8M6_001939"
     CDS             912..1112
                     /locus_tag="ACG8M6_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0457600.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACG8M6_001940"
     CDS             1259..4048
                     /locus_tag="ACG8M6_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0457601.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACG8M6_001941"
     CDS             4095..4622
                     /locus_tag="ACG8M6_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0457602.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACG8M6_001942"
     CDS             4643..5833
                     /locus_tag="ACG8M6_001942"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0457603.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8M6_001943"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACG8M6_001943"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0457604.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACG8M6_001944"
     CDS             7966..8640
                     /locus_tag="ACG8M6_001944"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0457605.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACG8M6_001945"
     CDS             8637..8978
                     /locus_tag="ACG8M6_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457606.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACG8M6_001946"
     CDS             9008..9367
                     /locus_tag="ACG8M6_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0457607.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACG8M6_001947"
     CDS             9627..9749
                     /locus_tag="ACG8M6_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457608.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACG8M6_001948"
     CDS             9891..11975
                     /locus_tag="ACG8M6_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0457609.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="ACG8M6_001949"
     CDS             12286..13752
                     /locus_tag="ACG8M6_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0457610.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4635 a         2430 c         2974 g         3939 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct acttctggaa
    13801 caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    13861 aaaaatagct cctcatattt tgccgttttt tgagctaatg ctgaaggtca gctgattgaa
    13921 caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgttttt
//
LOCUS       DAZKOB010000067        13964 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000067 DAZKOB010000000
VERSION     DAZKOB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.067"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            452..499
                     /locus_tag="ACG8M6_001950"
                     /pseudo
     CDS             452..499
                     /locus_tag="ACG8M6_001950"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            515..2434
                     /gene="tet(M)"
                     /locus_tag="ACG8M6_001951"
     CDS             515..2434
                     /gene="tet(M)"
                     /locus_tag="ACG8M6_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0457611.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2553..2720
                     /locus_tag="ACG8M6_001952"
     CDS             2553..2720
                     /locus_tag="ACG8M6_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0457612.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2780..3133)
                     /locus_tag="ACG8M6_001953"
     CDS             complement(2780..3133)
                     /locus_tag="ACG8M6_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457613.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3637..4056
                     /locus_tag="ACG8M6_001954"
     CDS             3637..4056
                     /locus_tag="ACG8M6_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0457614.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            4082..4288
                     /locus_tag="ACG8M6_001955"
     CDS             4082..4288
                     /locus_tag="ACG8M6_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457615.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(4516..4689)
                     /locus_tag="ACG8M6_001956"
     CDS             complement(4516..4689)
                     /locus_tag="ACG8M6_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457616.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            4701..5330
                     /locus_tag="ACG8M6_001957"
     CDS             4701..5330
                     /locus_tag="ACG8M6_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457617.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(5412..6578)
                     /gene="dnaJ"
                     /locus_tag="ACG8M6_001958"
     CDS             complement(5412..6578)
                     /gene="dnaJ"
                     /locus_tag="ACG8M6_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0457618.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(6729..8558)
                     /gene="dnaK"
                     /locus_tag="ACG8M6_001959"
     CDS             complement(6729..8558)
                     /gene="dnaK"
                     /locus_tag="ACG8M6_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0457619.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(8609..9172)
                     /gene="grpE"
                     /locus_tag="ACG8M6_001960"
     CDS             complement(8609..9172)
                     /gene="grpE"
                     /locus_tag="ACG8M6_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0457620.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(9266..10309)
                     /gene="hrcA"
                     /locus_tag="ACG8M6_001961"
     CDS             complement(9266..10309)
                     /gene="hrcA"
                     /locus_tag="ACG8M6_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0457621.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(10543..11708)
                     /gene="hemW"
                     /locus_tag="ACG8M6_001962"
                     /pseudo
     CDS             complement(10543..11708)
                     /gene="hemW"
                     /locus_tag="ACG8M6_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(11724..11951)
                     /locus_tag="ACG8M6_001963"
     CDS             complement(11724..11951)
                     /locus_tag="ACG8M6_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0457622.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12435..13042
                     /locus_tag="ACG8M6_001964"
                     /pseudo
     CDS             12435..13042
                     /locus_tag="ACG8M6_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            13215..13928
                     /locus_tag="ACG8M6_001965"
     CDS             13215..13928
                     /locus_tag="ACG8M6_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457623.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         4175 a         2685 c         2508 g         4596 t
ORIGIN      
        1 ttcttttctg ttgtgggcaa tcaagaggaa gaaagggata aaatctgatt tgtggtaaat
       61 caggcaaagg caacgcaggt atctcaattt gatttggtac ttgaaaagaa cgggagtaat
      121 tggaagatta tagaataaca aatattggta cattattaca gctattttgt aatcacgtac
      181 tctctttgat aaaaaattgg agattccttt acaaatatgc tcttacgtgc tattatttaa
      241 gtatctattt aaaaggagtt aataaatatg cggcaaggta ttcttaaata aactgtcaat
      301 ttgatagtgg gaacaaataa ttggatgtcc ttttttagga gggcttagtt ttttgtaccc
      361 agtttaagaa tacctttatc atgtgattct aaagtatccg gagaatatct gtatgctttg
      421 tatgcctatg gttatgcata aaaatcccag tgataaaagt atttatcact gggattttta
      481 tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt
      541 agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata acagtggagc
      601 gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata cgcttttaga
      661 acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg aaaatacgaa
      721 ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat atcgttcatt
      781 atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac aagcacaaac
      841 tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt ttatcaataa
      901 gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag agaaactttc
      961 tgccgaaatt gtaatcaaac agaaggtaga actgtatcct aatatgtgtg tgacgaactt
     1021 taccgaatct gaacaatggg atacggtaat agagggaaac gatgaccttt tagagaaata
     1081 tatgtccggt aaatcattag aagcattgga actcgaacaa gaggaaagca taagatttca
     1141 gaattgttct ctgttccctc tttatcatgg aagtgcaaaa agtaatatag ggattgataa
     1201 ccttatagaa gttattacta ataaatttta ttcatcaaca catcgaggtc cgtctgaact
     1261 ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg catatatacg
     1321 cctttatagt ggagtactac atttacgaga ttcggttaga gtatcagaaa aagaaaaaat
     1381 aaaagttaca gaaatgtata cttcaataaa tggtgaatta tgtaagattg atagagctta
     1441 ttctggagaa attgttattt tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga
     1501 tacaaaacta ttgccacaga gaaaaaagat tgaaaatccg caccctctac tacaaacaac
     1561 tgttgaaccg agtaaacctg aacagagaga aatgttgctt gatgcccttt tggaaatctc
     1621 agatagtgat ccgcttctac gatattacgt ggattctacg acacatgaaa ttatactttc
     1681 tttcttaggg aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt
     1741 ggagatagaa ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga
     1801 atataccatt cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt
     1861 atcaccgctt ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt
     1921 aaatcaatcg tttcaaaatg cagttatgga ggggatacgc tatggctgtg aacaaggatt
     1981 gtatggttgg aatgtgacgg actgtaaaat ctgttttaag tatggcttat actatagccc
     2041 tgttagtacc ccagcagatt ttcggatgct tgctcctatt gtattggaac aagtcttaaa
     2101 aaaagctgga acagaattgt tagagccata tcttagtttt aaaatttatg cgccacagga
     2161 atatctttca cgagcataca acgatgctcc taaatattgt gcgaacatcg tagacactca
     2221 attgaaaaat aatgaggtca ttcttagtgg agaaatccct gctcggtgta ttcaagaata
     2281 tcgtagtgat ttaactttct ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg
     2341 gtaccatgtt actaccggtg aacctgtttg ccagccccgt cgtccaaata gtcggataga
     2401 taaagtacga tatatgttca ataaaataac ttagtgtatt ttatgttgtt atataaatat
     2461 ggtttcttgt taaataagat gaaatatttt ttaataaaga tttgaattaa agtgtaaagg
     2521 aggagatagt tattataaac tacaagtgga tattgtgtcc tgtatgtgga aataaaacac
     2581 gattaaagat aagggaagat actgaattaa aaaaattccc cctctattgt ccgaaatgca
     2641 gacaagaaaa tttaattgaa ataaagcagt tcaaagtaac tgtgattaca gagccagacg
     2701 caaagacgca gagccgataa aatgagatta atacaatctc attttatcgg ctctttccgt
     2761 tatgtatgga ttcttttaat tagtcttcga tgtttcttgc ttcgttgata ccgctggcta
     2821 aagattccat taaggatagt tctttgtctg taaagctatc catgtatttc tctatctgta
     2881 atcgtcgggt gctttttacc aagttattag caggtaagaa aaattcatca acggaaacat
     2941 gaagtaacga tacaaggtca taaagaactt gtatgctggg gtgttgccct ttattttcaa
     3001 tattagttaa gtaccgtggg tcaatttcaa tcaatgctcc cacttgttca cgagttaaac
     3061 ctcgtttcaa tcgagcttct ttaatggcta aaccaaaggc tctaaaatca tatttatctt
     3121 cttttttacg catagtagac cacctctata cattttattg ttcctactga attaaaaaca
     3181 ggtatagaaa aacgtgttat atggtttata ggtttatatt taataaaaag cactactaaa
     3241 cgccaataaa aaaaaccgtt atatggtagt gctatttacg ctgttaaaat attgtatatt
     3301 acttccaaat ggcggtttgt tggaggtcaa cgtcgccatg aagtacatca tatacaataa
     3361 atttccttac attgggttct tgtcaaaaaa agtcgtctat ctgcaataga taagtacgtc
     3421 caccaatgtg gttttataaa tcatatagat agaataacag aagcatgtaa acagagaaat
     3481 aaatctgttt atatgctttt ttggctattc agaacttttt tacaaagttt atttatcagt
     3541 aatgcaacaa atcccccttt cacattggga ctaagagtga aaggagataa acgagcaagg
     3601 ctcacttcct ttcctagaca gaaagggggt gagattatga ggacatcttc tttcgagcat
     3661 atcgtcagat tacagttcaa ttctttaatg ctgattgtca ttaagaacaa agtgaaaagt
     3721 cgaaacagac agcttgcaag acattctaaa cgtgaagtat tgttttgtga aatagccgaa
     3781 acgaaacaag ttgaacgtgg aacgattgat acctgttttt atgattgtgt atcatttaaa
     3841 gtcttgcact ttacaattta tgtatctgat gaaacacttg gtacagtttt acatagacta
     3901 tcagaaaaac agcgtagtgc gattttatta cgttattttc aaggaatgaa cgacagagaa
     3961 atttcagaac tgtaccatgt atcacgttct gctatttgca gtagaagaac tagaggacta
     4021 aggaagttaa aaatgctttt gagcgaaagg aagtaaagat tatggctatc cctcttttga
     4081 attgatttgc aaagcgtcaa gcggagatga aatggcagtt aaagagattt tgaagtttta
     4141 tgacggttat atttctaaat tatgcttgcg tcctttttac cactctgaaa gtggtaaaat
     4201 cattatgcag gttgatgaag aactaaaagg agaaattcat acagacatga tgaaagcaat
     4261 attgaaattt gaaatcggag tgaagtaatc aactgtataa aaaaatcggg agatacacag
     4321 tttcgtgtta tctcccttag tagttttacg gtcatatcaa ggctcattca atcgtggtaa
     4381 attcgtggta agatgagtgt ttttctatac ttcaaaatgc ttgaatgtat agtgtttatg
     4441 cggataattg aaaattagat ataaaattta ataaaaacca aaaatgcacg gtcgccatga
     4501 agggaccgtg tatttttatc ttcttatttt ctcacctaat cgtatcagcc gcatcgcttg
     4561 ttttcctgca caaacaagca atcgctctac ctgttccatc gcataagaaa gtaccatcgg
     4621 ttcattgatg atatcaagta catagtcgat accaccctca cttaattgag cttgattttt
     4681 cttctccata ttatgtagtt gtgggacaac tttctgacaa tcagcaattg tgtcaagaac
     4741 ggatcaatcc tttattggag ttgctcgaaa gggtcttctc ttattacgga caggctttgc
     4801 tcactgtaca tagacaacaa atcattatcc tagtcaatcg gatttccaga gcctcattgc
     4861 tatcattgct tgataaaatc cagacaaagt ttaataaaat gtatcagtta cagctaaact
     4921 ttggaattgg gtcgctttgt tatacagagc aagagacacc gcaatctttc cttcatgcca
     4981 aacaagtttg cgaatggata gctttccatc aatctgtgaa cgagatccgc ttttttgaag
     5041 atttagacct cgggatcgtt ttaccagcaa tcccgtcaga tcaacgaaca ttatatgtaa
     5101 aacgtatttt gaaaagcctg acagaagaag aagtacattt gttcaagaag actttggcat
     5161 gcttcagtaa aaataacgga agcatcaaaa attgctcaga agaattattt attcataaaa
     5221 acaccttgca atatcgattg aacaagttcc attctctcac tggttatacc cctagaaact
     5281 atgacgatta tcatattcta aagctggctt ttttgttggt acaaacatga caactacaaa
     5341 aaaaccaggc gaacatatga ttgttcgtct ggtttttaat tattaatgac ttagcgtttt
     5401 tactagaaaa actacttccg ctttttcccg ccaaaagcat ctttaaactt atcgaagaaa
     5461 ccgtcttctt gctgttcttc aactttgatg ccgccagctt cagcgaatgc acgtaatgct
     5521 tctctttgtt gttcatttag atttttcggt gtgatcagtt ttactttgac atgttggtcg
     5581 cctgttgcac caccacgtaa cttcggagcg ccttttccac gtaaacggaa gttcgtacct
     5641 gtctgtgttc cagcagggat cttcaatttc acatctccat ggacagttgg tacttggact
     5701 tcatccccca aagctgcttg tacaaaactt aaaggcaatt catagtaaat ttcagaacct
     5761 tcacgatcaa agatatcact ttcttctaca cggaagacca catacaaatc accaaatggt
     5821 ccgccgtttt ctccagcttc tccttgacca gctaaacgca tttgttgtcc ttcttctaca
     5881 ccagctggta cattcacttt aactgaatgt gctttcttct catgtcctga gccatgacag
     5941 gttggacaag gttctttgat ttctttccct gtcccatgac aaacatcaca agtttgacgg
     6001 ctcatcactc gtccaagtgg tgtttggcgt tctacattaa tcgtacctgc accatgacat
     6061 ttatgacaag tagttggttg tgtgcctggt tttgcaccgt tcccaccaca agtatgacag
     6121 atttcttccc gattatactt gatttctttt tctacaccaa aaatcgcttc ttcaaaagac
     6181 aagtcgaccg tatattgcaa atcagcacct tgacgaggcg catttggatc gactgaccga
     6241 ccgcctcctc caaagaaaga gtcgaagata tcttcaaaac caccgaagcc gccaccagag
     6301 aagccgccga atccaccaaa tccgccacca gctccaccag caccgtaatt tggatctgtg
     6361 cctgcatgcc cgtattggtc atatgcagcg cgtttttgcg ggtcacttaa aatctcatat
     6421 gcttctgata cttctttgaa tttttcttcc gcatcaggtt ctttattgat gtcaggatgg
     6481 tattttttcg aaagcttacg ataagctttc tttatttcat cttctgatgc tccttttgag
     6541 agccctaaga cttcataata atcacgtttt gttgccatag gctttcctcc tattggttct
     6601 catcattttc acgtcgcttg ttatcatatc atattttgtt tgaaacaaaa accgatactt
     6661 caaactattt ttcaaaagaa aaagccaaag tagcaacttt ggccttctct cgaaattcag
     6721 tgaactgatt atttatcgtc gccatcgact tcttcaaaat cagcatcaac gacatcatct
     6781 gctccgcctt gagctgcttc tgggttttct tgagcttgtt gttgtgccgc ttgttcgtat
     6841 agtttaacag tcaagttttg aacgatttcg ttcaatgcat cgcgtttagt tttcatttct
     6901 tcgatgttgt ttgcttcaac agcggctttc aattcatcgc gagctgtttc tgcttttttc
     6961 acttcttctt cgtccacttt gccttctaat tctttcaatg ttttgtccac agagaatagc
     7021 aatgcatcta catcattacg taggtcaact tcctctttac gagctttatc tgcttctgca
     7081 tttgcttctg catcttttac catacgttcg atttcttcgt ctgttagacc tgaagaagat
     7141 ttgatcgtga ttttttgttc tttttgtgta cctagatctt tcgcagatac atttacaatc
     7201 ccgtttttat caatatcgaa tgttacttcg atttgtggaa taccacgagg agctgctgga
     7261 atatctgtca attggaatct tcctagagtt ttgttgtcag cagccattgg gcgttcacct
     7321 tgtaatacat ggatgtctac tgctggttga ttatctgccg cagttgagaa tacttgtgat
     7381 ttacttgttg gaatcgttgt attgcggtca atcaatttag tgaacactcc acccattgtt
     7441 tcgataccta atgacaatgg tgtaacgtca agtaaaacaa catctttcac atcaccagtg
     7501 atcacgccac cttggatagc agcacccata gcaactactt catcagggtt aactgatttg
     7561 tttggttctt ttcctgtttc tttacgtact gcttctacaa ctgcagggat acgagtagat
     7621 ccaccgacta aaataacttc gtcgatttct gattgtgata aacctgcatc ttttagcgct
     7681 tgacgtactg gtacttttgt acgttctact aagtcagctg ttaattcatc aaattttgca
     7741 cgtgtcaatg tcatttccaa atgcaatggt ccagattctc ctgctgtgat aaatggcaag
     7801 ctgatttgtg tgcttgatac gccagataaa tcttttttcg ctttttcagc agcgtctttc
     7861 aagcgttgta aagccatttt gtcttgagac aagtcaatgc tgttttcttt tttgaattct
     7921 gctaccatgt agtcgatgat tttgttatca aagtcgtccc cacctagatg gttgtcgcca
     7981 gctgttgaca atacatcaaa gacaccgtct cctagttcaa ggatagatac gtcaaaagta
     8041 ccgccaccaa ggtcgaatac taagattttt tcgtcgcgat ctgttttatc taaaccatag
     8101 gcaagagcag ctgcagttgg ttcattgacg atacgttcta cttctagacc agcgatctta
     8161 ccagcatctt ttgttgcttg acgttgtgca tcattaaagt aagctggcac agtgataact
     8221 gctttttcga ctttttcacc taaatagtct tctgcgaatc ctttgatata ttgaaggatc
     8281 attgctgaga tttcttgtgg tgtatatgat ttaccttcta catctacttt gtaacctgct
     8341 tcaccgatat ggcgtttgat tgatgaaatc gtatttgggt ttgtcacagc ttgacgtttt
     8401 gcaacttccc ctacttggat ttctccattt ttgaatgata caacagaagg tgttgtacgg
     8461 tttccttctg ggttagcgat gatttttgct tcgtttcctt ccaatactgc tactgcagag
     8521 ttagtcgtac ctaagtcaat accgataatt ttactcataa ttaacgtctc cttctttaat
     8581 tgaaagtgta agttttatat ccgcacaatt attgtgcaac aatgaccatg gttggtctta
     8641 acacgcgatc atgtaatttg tagccttctt gtagtacttc tacaatcgta tcggctggtg
     8701 tatcttcagt cgctggaaca gtttgtactg cttgatgaag attaggatca aaggcttctc
     8761 ctttagcagg gattttttca attccttctt cttccagtgc gttacgcaaa ctttccaaga
     8821 ccatttccac gccttttttc aaacctgccc cttgatcatc tgacacttct gtggctaatg
     8881 cacgttccag gttgtcaatt gatggaagta attttttggc taaatcttgt gaacggtatt
     8941 tttgtaattg ctcacgctca ttttttcctc gatttgccat atttgcgatt tcagcacgag
     9001 cccgcaagta tttatcttcc atttcttcta actcagcttt taatttcgct gtttcgaatt
     9061 cttccgcttc agcttctgaa atacccgaat cttctgcagc ttcgacatcg atttccggtt
     9121 ctggctgagc atctgtcatt tctttatcta gttcttcttg attttctttc acgatacagt
     9181 cttcctttca ttattcagtc agctgtttga ttgctcttac taagaaagag agaacgatca
     9241 gctgcaaata gcttcgttat tcacattacg agtttgacaa gtcaagcgaa cggtagtaat
     9301 cagcaagttt agctgctagt tctcttcgat aaacatcgac tagaccgaac atccgagaat
     9361 aaggcatact agtaggccct aacaaggcta ttgtacctct tccatgacca tctatctcgt
     9421 aacttgcttg aatcatactc atattctgca ataaatcatt gcctaattca gcaccaatct
     9481 tgatatgaat attgctatcc atcggagtga tcatttgtgt aagtccgtct gagtctttca
     9541 taaaagaaaa catcgatttg aactgatcaa gatcttgacg gggctcataa tccaagagat
     9601 tcatttgtcc actgacataa accttatctt caaaaacatg accaagaacg gtgtcaaata
     9661 actgagacat cccttctgtc gtttggaaat atttatgaag aatcatcgga atctccgtac
     9721 gaagtttatg gtacaccgtc atcagaggat ctccgactag acggtcgttt acaatctgta
     9781 ccattttttc tagttcctga cttcccatat attcgggaag agtgaacact tggctttcta
     9841 cattccccat atctgttacg acgatagcaa taatctgtcg attattcaag ggtacgatac
     9901 gaaagccagt cagtcggcgg tcttttattt caggtcccaa tgaaaaagtc gtatagcttg
     9961 tcagctcaga taatatctca gcagattgct taataatctc attgatttca tgaaattctc
    10021 tgcctagaga ctgacgaatc agcagcaaat catcttctgc tacgcgagca ggttttaaca
    10081 aatgatccac gtaataacga tagcctaaag cagatggaat ccgcccagaa gaagagtgag
    10141 tcttcaacag caatccttca tcttctaatg ctttcatatc attgcgaatc gttgctgagc
    10201 tggccttgac tccctcagac atcaatgttt tggaaccaac gggcacgcca ctactcgtat
    10261 agtgttggat gatcagacgt aaaatgtcgt tttgtcgctt tgttaacatc ttcctcacct
    10321 ctcattagca ctcatatatt ctaagtgcta atccacttat aaatatagca agaagccctt
    10381 ctcctgtcaa gagaaagact ccatttttta gcactctaaa cacaagagtg ctaattctaa
    10441 ataattgatg taaatccagt tatatcaacg tttcaatagt cctttattgt atttttgaac
    10501 tttttttatt taaattcaga taaaaaacaa atattcttta aactattttt caaacaaaaa
    10561 gcgctcaaac acattgttcc ccaaaaaggt ccctcttggt gtcaaataaa cacggtcttc
    10621 ttcattaaca agtaaccctt cttgctctaa ttcccttagc acatcaccgt acaccgattc
    10681 aattgttttt tgatagcgat catagaaatg ttttttagta atccctgttt ttttcttagt
    10741 cctaagaaca tttcttcttc tatttgattt tttcgtgtca atttttcttc ttccaatacc
    10801 gggcgttcat ttgctcgtaa tgggcgtaaa tagtgttgga ttggtccttt gttgcggtaa
    10861 cgaatatctc caagataacc gctagcacca gctccaaacc caaaatagtg gtcattgttc
    10921 cagtacatga ggttatgttg agattcatgt ccggagagcc caaagttact tatttcatat
    10981 tggtgacgtc catgcttttc cattgcttca atcgtctctt caaacattct ggtttccgtt
    11041 tcctgatcag gcagttcgag tcttccttgt cgtacccagt tcatgaacat tgttttgttt
    11101 tcgaggatca atgagtacaa agaatagtga ggcagatcca aagccaaagc tttttccaaa
    11161 gtatccagat atccttctag tgtttgtccc ggtaatgcat aaatcaaatc gatgcttaca
    11221 ttggtaaaat tttctgcttc taaaaattgc atagtttgat acacatcttc tgcggtgtgt
    11281 ttacgtccaa tttttttcaa taagcgatta tcaaatgtct gcactcccat ggataaacga
    11341 ttgacgccat agtttttcat tacttgaagt ttttcacggg tcaaatcacc tggattcgct
    11401 tctacagtaa actcattacc attttgaaaa ggcaggatct ctctagcacc tgataacagt
    11461 cgatcaagtt gtttagcaga taaagaagtt ggtgtcccgc cccctacgta aagtgtttct
    11521 aaattcgctg tcggatgact ttctagcatt attttcattt cattcagcag catttccaca
    11581 tactcatcta ctggctgccc ttctaaaaaa actttattaa aatcacagta atagcagata
    11641 tgctcgcaaa atgggatatg aatgtacgca gaaaccttgt tttctgcggg atttattgtt
    11701 tctatcattg aaattctttc tccttacgtc gaaaagtcgt gcctgttttc gaccttctta
    11761 aaatcgcgtt caagcaccaa ctcattgaaa taagccgcat atttagaatc aaggaaacct
    11821 agctctaaaa atgcttgctt attgtcatcg gagctacacg gtgcttttac agcctctcga
    11881 taaacggtaa tcgcaaagct gaccttcggc attaagtcaa aatctgcaaa aatatgaaaa
    11941 gctattttca cagattcctc cttaagtgct cagaccacaa cgctttgctt acaacctctc
    12001 atatacgcat actaaacgta tttggcgaac aaatccacta aaaaatccaa gaatcgactt
    12061 cttggatgat tttttctttc attaaactgg ttacctagtt atatatctct agggttgttt
    12121 tcatgtctga gtgcccgata tgatttattt ttcgtttcta taattacttt ttgccttcca
    12181 gtttctttta atattgtact caagcaagtg ggaattggtt cttttgtcat ccatgtatgc
    12241 aaaatgttat aatgctggta aacaaaaatg attacctgtc atttttattt agttcgtatt
    12301 taattaagtt gactatctgt atactctgtc taaaacttaa gaattgttgt tttcgctgtc
    12361 gctacccaat tttaatgaat gtctgtaggt taaaactgtt gtccttgtta tagatagtaa
    12421 aggagagctt gaaaatgaaa actgcgatta cgtttctatt agaggaatat gccgattggg
    12481 aaggtgctta tattactagc acactaaacc aaaatgagaa atggcaaaac aaaactgctt
    12541 ctatccaatc agaggtcact tccattggag gattaaaaac aaaaatcgat tacttaatcg
    12601 aacaaatacc tgaaaaagtt gaccttttta ttatgatcgg tggcaattca tgggacataa
    12661 aaaatcatca tttatatcaa ataatcgata aattgttaaa aacagacgta ccagtagctg
    12721 ctatttgtgg ggcagttgat tatttagctc agagcggatt attgcaaggt tacaaacaca
    12781 ccggaaatgc gcaatttcct tggttagaat cggaaaatta tcagaataaa gaagatttta
    12841 tagaacaaca agctgtgaaa gatcgtaatt tgattactgc gaatggaaca gctcctatag
    12901 aattcacaga cttgattctt caagcagtcc attttgaaac agaagaaata agagaaaaaa
    12961 cgattcgtct atacaaactt ggattctatg attactataa aaagtacgga gatcctttct
    13021 cctaatcatt ctcttcaatt aaatactcct tatcaatagt gtgacaagta attttactga
    13081 tgattatttg tcacactttt tctgacattt atcaaagaag atacactcta tttatattca
    13141 tagacaaggt tttacggatt caaaaattca ggattatgct atactggtat taaaaaaata
    13201 atgaggtatt ttatatgaaa aagttaggtt ttattttatt gttggtctct ttattccttg
    13261 taggatgtac gtctcctagt aaagaaaata gtgatccatc tgcgaccaca gacaaaagtt
    13321 caatcagaca aaccagttca gctgaaaaat caagtactca gaaatcagct tctactgaaa
    13381 cgaatacatc ttcaacaagc agtaaagtct cagagcccaa tacttcttct acatctagta
    13441 gtacagttga gacaaatatt tcttcttcaa caatagacac aacctcctca cagtcttctc
    13501 agtcaataga tccgttagca ggatattctg atctacaaat tgagtacgca cgtgtttggc
    13561 tagctattag aggtaatact tttagagata catcgggtga tttcgaatta catgttattc
    13621 attatcctgc aggaacacct atcgcccctt atgatgcagg gagtgcagtc tatccgaaag
    13681 atgtggttat gctcactggg aaatacagtt accagggagt tttagtgtat tcaagtaacc
    13741 atgacgggac aatcactagt tatccagttc cttcacattg gcaaatgcct gcagatcaaa
    13801 catcttatcc agcgttcatt agagaaacaa ctcaagaaat cattgataat gcatctgtag
    13861 tagctattcc tacaggaaat cccaatgacg tgaaacagtt aatcgatgtg acagttatag
    13921 ataattagac tttctaatat gttatacaaa aagaagctga cagc
//
LOCUS       DAZKOB010000068        13724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000068 DAZKOB010000000
VERSION     DAZKOB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.068"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1364)
                     /locus_tag="ACG8M6_001966"
     CDS             complement(45..1364)
                     /locus_tag="ACG8M6_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0457624.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="ACG8M6_001967"
     CDS             complement(1361..2014)
                     /locus_tag="ACG8M6_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0457625.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="ACG8M6_001968"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="ACG8M6_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2820..3539)
                     /locus_tag="ACG8M6_001969"
                     /pseudo
     CDS             complement(2820..3539)
                     /locus_tag="ACG8M6_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(3564..3749)
                     /locus_tag="ACG8M6_001970"
     CDS             complement(3564..3749)
                     /locus_tag="ACG8M6_001970"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0457626.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="ACG8M6_001971"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="ACG8M6_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="ACG8M6_001972"
     CDS             complement(5356..6387)
                     /locus_tag="ACG8M6_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457627.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="ACG8M6_001973"
     CDS             complement(6394..7224)
                     /locus_tag="ACG8M6_001973"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0457628.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="ACG8M6_001974"
     CDS             complement(7221..8036)
                     /locus_tag="ACG8M6_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0457629.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="ACG8M6_001975"
     CDS             complement(8033..8857)
                     /locus_tag="ACG8M6_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0457630.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="ACG8M6_001976"
     CDS             complement(8847..9947)
                     /locus_tag="ACG8M6_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0457631.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="ACG8M6_001977"
     CDS             10125..10910
                     /locus_tag="ACG8M6_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0457632.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="ACG8M6_001978"
     CDS             10943..11326
                     /locus_tag="ACG8M6_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0457633.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="ACG8M6_001979"
     CDS             11402..11533
                     /locus_tag="ACG8M6_001979"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0457634.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="ACG8M6_001980"
     CDS             11502..11738
                     /locus_tag="ACG8M6_001980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0457635.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11750..11836
                     /locus_tag="ACG8M6_001981"
                     /pseudo
     CDS             11750..11836
                     /locus_tag="ACG8M6_001981"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11816..12787)
                     /locus_tag="ACG8M6_001982"
     CDS             complement(11816..12787)
                     /locus_tag="ACG8M6_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0457636.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13439..>13724
                     /locus_tag="ACG8M6_001983"
     CDS             13439..>13724
                     /locus_tag="ACG8M6_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0457637.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT         4163 a         2983 c         2379 g         4199 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
    13381 gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag tgatagctat
    13441 gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga ttgatgatct
    13501 agttcctcaa gatcatctcg ttcgaaagat tgatgccgcg cttgattttg aatttattta
    13561 tccaatcgtc gaagccactt actccgattt aggtcgccca agtatcgatc cagtgattct
    13621 gatcaaattg gtgtttattc agtatttatt cggtatccgt tccatgcgac aaaccattaa
    13681 ggaagtcgat accaatgtcg cttaccgctg gtttttaggc tact
//
LOCUS       DAZKOB010000069        13685 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000069 DAZKOB010000000
VERSION     DAZKOB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.069"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..303
                     /gene="yaaA"
                     /locus_tag="ACG8M6_001984"
     CDS             61..303
                     /gene="yaaA"
                     /locus_tag="ACG8M6_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0457638.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            290..1414
                     /gene="recF"
                     /locus_tag="ACG8M6_001985"
     CDS             290..1414
                     /gene="recF"
                     /locus_tag="ACG8M6_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0457639.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1411..3357
                     /gene="gyrB"
                     /locus_tag="ACG8M6_001986"
     CDS             1411..3357
                     /gene="gyrB"
                     /locus_tag="ACG8M6_001986"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0457640.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3377..5848
                     /gene="gyrA"
                     /locus_tag="ACG8M6_001987"
     CDS             3377..5848
                     /gene="gyrA"
                     /locus_tag="ACG8M6_001987"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0457641.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6084..6383
                     /gene="rpsF"
                     /locus_tag="ACG8M6_001988"
     CDS             6084..6383
                     /gene="rpsF"
                     /locus_tag="ACG8M6_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0457642.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6433..6945
                     /gene="ssb"
                     /locus_tag="ACG8M6_001989"
     CDS             6433..6945
                     /gene="ssb"
                     /locus_tag="ACG8M6_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0457643.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6971..7207
                     /gene="rpsR"
                     /locus_tag="ACG8M6_001990"
     CDS             6971..7207
                     /gene="rpsR"
                     /locus_tag="ACG8M6_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0457644.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7350..9323
                     /locus_tag="ACG8M6_001991"
     CDS             7350..9323
                     /locus_tag="ACG8M6_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0457645.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9329..9781
                     /gene="rplI"
                     /locus_tag="ACG8M6_001992"
     CDS             9329..9781
                     /gene="rplI"
                     /locus_tag="ACG8M6_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0457646.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10055..11422
                     /gene="dnaB"
                     /locus_tag="ACG8M6_001993"
     CDS             10055..11422
                     /gene="dnaB"
                     /locus_tag="ACG8M6_001993"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0457647.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12202..13494
                     /locus_tag="ACG8M6_001994"
     CDS             12202..13494
                     /locus_tag="ACG8M6_001994"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0457648.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4561 a         2477 c         3013 g         3634 t
ORIGIN      
        1 tattgcgaaa aaaaaggtat aatagagtat acttttaata atgaaaatga gggatgggca
       61 ttgaaacaac aagtcattct aaatacggaa ttcatgacgt tgggccaaat gttgaaagaa
      121 gtcacagtta tcggtagtgg cggacaagca aaatggtatt tggcagaaaa cactgtgtta
      181 gtcgatggcg aaccagaaaa tcgtcgtgga cgcaaacttt atcccggcat gatggttgaa
      241 gttccagaag taggaacttt ttttatggta aaaaaaggaa acgacgataa tgaggctgaa
      301 taagctgtat ttaaaaaatt atcgaaatta cgaagagctg aacatcgaat tttccaagaa
      361 cctcgtcatc tttttaggag aaaatgcgca aggaaaaacg aatcttttgg aaagtatcta
      421 cgtattagct atgacgcgaa gtcatcggac aagtagtgag caggaattga ttggctggac
      481 tgacgatcag gcgatgattc aaggagagat cacaaaaggt tcatccactc ttccattaga
      541 aatcttatta tctaaaaaag gccgaaaaac caagatcaac catatcgaac agaaaaagtt
      601 aagtacttac gtgggacaaa tgaatgtcat tttatttgca ccagaagatc tttctttagt
      661 aaaaggcagc ccgcagatca ggcggaagtt tttagatatg gaattaggcc agatcaatcc
      721 aatctatctt tatgatcttg tccaatacca agcaattttg aaacaaagaa accagtactt
      781 gaagcaattg aatgaaaaaa aacaaactga tttgctttat ttggatattt tgtccgaaca
      841 actagcagcg tttggcagca aagtattgaa agcaagagca cagttcgtca agcgattgga
      901 gttttgggct aattctttgc atcagcaaat cacccatcaa aaagagcagc tggaaatcga
      961 gtatctcacc gcagtcgatt ctttagaaac acatacgcaa gagcaaatcc aagaacaatt
     1021 tctcgcactt ttgaaccaaa ataaaaagaa agaccttttt cggggaacaa cgacagttgg
     1081 tcctcatcgt gacgatcttt ccttttttat taatcagaaa aatgtgcaga cttatggatc
     1141 gcaaggacag caaagaacaa ctgcactaag tgtcaaatta gcagaaatcg atttgattaa
     1201 agaagagaca ggggaatatc caatcctttt gttagatgat gtgatgagcg agctggatga
     1261 taatcgtcaa ctccatctgc tagaaacaat cgaaggaaaa gttcaaacgt tcttaacaac
     1321 aacgacactt gaccatgtaa aaaataaaat gactgttgaa cctgagattt tttatgttca
     1381 acaagggaaa ctaaaaggag aagaaactga atgacagaag aaagaagttt agtagaacgc
     1441 gctaaagagt atgatgccag tcagattcaa gtacttgaag gacttgaagc cgttcgtaaa
     1501 cgacctggca tgtatatcgg ttcgactagt tctgagggtc tgcatcacct tgtttgggaa
     1561 attgtcgata attcgatcga tgaagtatta gcaggttttg caacgaaaat ccatgtgatt
     1621 attgaaaaag acaacagtat caccgttatt gacgacggtc gggggatacc tgtagatatc
     1681 caggcaaaaa ctggacgtcc tgcagttgaa acggtcttta ctgttttaca tgctggagga
     1741 aaattcggcg gtggtgggta caaagtttct ggtggacttc acggagtagg atcttctgtt
     1801 gtaaacgcgc tttctacaca gcttgatgtc aaagtataca aagaaggaaa tgtgtactat
     1861 caagaatatc gtcgtggagc tgtagtggac gacttaaaga ttatcgaaca aacggatcgc
     1921 catggaacaa cggttcactt tacgccagac cctgaaattt ttacagagac aactgagttt
     1981 gatttttcaa aattggctac ccggatccgt gaacttgctt tcttgaaccg cgggatgcgt
     2041 atatcgattg aagacaaacg agaagaagaa ccagtcatca atgaatacca ttatgacggc
     2101 gggatcaaaa gttatgtcga atacttgaat gcaaacaaaa ccgtgatttt tccagaacct
     2161 gtatatttgg aaggtgaaca gcaagatatc gcagttgaag tctctatgca atatacagac
     2221 ggctaccatt ccaatatcat gagttttgcc aacaatatcc atacgtatga aggtggaaca
     2281 catgaatcag gattcaagac tgctttaact cgtgtgataa atgattatgc ccgaaaacaa
     2341 aagctgatga aagaaaatga cgacaacttg accggtgaag acgtgcgaga aggattgaca
     2401 gctgttattt ctatcaagca tcctgaccct caattcgaag gacaaacaaa aacaaaattg
     2461 ggcaattccg aagtacggac agtcactgat cggttattta gcgaacattt catgaaattc
     2521 ttgctggaaa acccaagtgt gggccggcaa attgttgaaa aaggattact agcttccaga
     2581 gctcgtctag cagcgaaacg tgcgcgtgaa gtaacgagac gtaaaggtgc tttagagatt
     2641 agtaatctgc caggaaaatt agcggactgc tctagcaacg atcctgaaaa atgcgaattg
     2701 ttcatcgtcg aaggagattc ggccggcgga tcagctaagc aaggtcgtaa tcgagagttt
     2761 caagccatct tgcctattcg tgggaaaatc ttgaatgttg aaaaagcaag catggacaag
     2821 atcttggcaa atgaagagat ccggtcttta tttactgcta tgggtacagg gtttggagaa
     2881 gattttgatg tatcgaaagc tcgctaccac aaattagtta tcatgactga tgccgatgtc
     2941 gacggcgcgc atatccgtac attgctgctg actctttttt atcgttacat gcgtcctatt
     3001 gttgaagctg gatacgtcta tattgctcag ccacctttat acggggtaaa acaagggaag
     3061 aatattacgt atgtacaacc aggaaaaaat gcagaagaag agttgaaaca agtcgttgct
     3121 tcacttcctg cctcaccaaa accaagcgtt cagcgttata aaggacttgg agaaatggat
     3181 gatcatcaac tatgggaaac aactatggac ccaagcaatc ggatgatggc ccgcgtcagt
     3241 gtggacgatg ctattgcagc agatcagata tttgaaatgt tgatggggga tcgagtagaa
     3301 ccccgtcgtg cattcatcga ggaaaatgca cactatgtga agaacttaga tatttaatca
     3361 tgaaggagga aaaagaatga gtgaagaaat cagagaaaac atccatgatg tcaatctaac
     3421 cagcgaaatg aaagactcct tcatcgatta tgcgatgagt gttattgtcg cacgtgcact
     3481 tcctgatgtc agagatggtc tgaaacccgt tcatcgccgt attttatacg gcatgaacga
     3541 attaggtgta acaccggata aagcccataa aaaatctgcc agaatcgtcg gagatgtcat
     3601 gggtaaatac catcctcatg gagacattgc gatctatgag tcaatggtac ggatggcaca
     3661 gccattcagc taccgttata tgctagtgga tggtcatgga aactttggtt ctgttgacgg
     3721 tgacggtgcc gccgctatgc gttataccga agcacggatg agcaaaatcg ctacagaaat
     3781 gctgcgtgat atcaataaga acactgttga tttccaaagc aactatgatg atacagaaaa
     3841 agaaccagtt gtactacctg ctcgcttccc gaatctttta gtgaacggaa caacggggat
     3901 cgccgtaggt atggccacga atatcccacc tcataacttg tctgaggtag tagcggctat
     3961 cgatttgctg atggataatc ctgatgtgac gaccaatgaa ttgatggaag tattacctgg
     4021 accagatttt ccaacaggtg gtttggttat ggggaaatca ggaatccgaa gagcgtatga
     4081 aacaggtaag ggttctatta ccgtacgtgc aaaagttgag attactgaga tgccaaatgg
     4141 taaagaacgg atcatcgtga cagaattgcc ttatatggtc aataaagcaa aattgatcga
     4201 acggatttct gaactgcaca gagacaagcg tatcgaaggg atcacagatt tgcgggacga
     4261 atcttcccgt gaaggtatgc ggatcgtcat tgacatccgt cgtgacgcca gcgcatccgt
     4321 tatcttgaat aacttatata aattgacatc cttgcaaaat tcatttggat tcaatatgtt
     4381 ggcgatcgaa aaaggtgtgc cgaaagtttt gagcttgaaa caaatccttg aaaactatat
     4441 cgaacatcaa cgcgaagtca tcacacgacg gacggtcttt gaaaaagaaa aagcagaagc
     4501 aagagcacac atcttagaag gtttgcgtat tgctttggat catatcgatg aaatcattgc
     4561 gattatccgc gggtctaaat cagatgacga ggcaaaagca acaatgatcg aacggttcga
     4621 tttatccgat cgccagtctc aagctatttt agatatgcgt ttacgccgat tgaccggttt
     4681 ggaacgggaa aaaatcgaaa atgaatacca agaactctta aaactgattg aagatttaac
     4741 tgacattctg gcaagaccag aacgtgttac agaaatcatc aagaatgaat tagcagactt
     4801 gaatcaacga tttggtgata aacgacgcac agaattacta gtcggtgagg tcttaagtct
     4861 ggaagatgaa gatttaatcg aagaagaaga aatcgttatt acattgacaa acaatggtta
     4921 catcaaacgt ttggcaaata atgaattcag agctcaacga aggggtggac gtggtgtcca
     4981 aggaatgggt atccacaatg acgacttcgt caaaaatctg gtttcttgtt ctacccatga
     5041 tacattgtta ttctttacga ataacggaaa agtgtatcgg gcaaaaggat acgaaattcc
     5101 agaatacggg cgtacagcca aaggaatccc aatcatcaat ttactaggta tcgattcgag
     5161 tgaaagcatc caggcaatca ttgcggtgac gggagaagca gaagaaggcc actatctctt
     5221 ctttacgacg cgtcaaggta cagtaaaacg tacttctgtc actgcttttg ctaacatccg
     5281 aagtaatggt ctgatcgcaa ttggtttgaa agaagacgat gagctcgtca atgtgctact
     5341 gacagatggt caatctaata tcattattgg aacacataac ggctactctg tcactttcaa
     5401 agaagaagca gtccgcgata tgggacgtac agcttccggg gtcagaggga tacgcctgcg
     5461 tgaggaagat tatgtcgttg gggcagcctt aatgaaggaa aatcaagaag tattagtgat
     5521 cacagaaaaa ggttacggaa aacggacaaa agtatcggaa tatccagtga aaggccgcgg
     5581 aggtaaaggg atcaaaacgg cgaacatcac tgaaaaaaat ggtccactag ctggattgac
     5641 cactgtttct ggagatgaag acatcatgct gattacagat aaaggtgtca ttatccgttt
     5701 caacgtatcg actgtttctc aaactggacg ttcaacatta ggcgtacgtt tgatgaagat
     5761 ggaagctgat acgaaagttg tcacgatggc agcagtcgaa ccagaagctg cagaacaaac
     5821 agttgaagaa caaggatcag aagaataacc atcaagacta agtgtgtaag ctagcaagaa
     5881 taaccgatta aaaaatctgg ttattcctgc tggctttttt gattagttga atttctctta
     5941 aaatattcga aactgttcat tctgtattgc ttttgaccaa aatagagtgt ataattagag
     6001 cttgtgagta gtctaattaa ttagataact ctccttgctc ttaggaacct aagagccgaa
     6061 agtccacagg gaggtgaata tcaatggaaa acacgaaata tgaaatcatg tacattattc
     6121 gtccaaacat tgatgaagaa gcaaaaactg ctttaatcga acgtttcgat gcaatcttga
     6181 aagataacgg agcagaagtt atcgaatcaa aagattggga aaaacgccgt ttcgcttatg
     6241 aaatgaacgg ttaccgtgaa ggtatctatc acatcgttaa agtatcttct ccatcatcag
     6301 caaatgctgt caatgaattt gaccgtcttg ctaaaatcaa cgacgatatc attcgtcaca
     6361 tgattgttaa agaagaagct taattgttgt ttcatgtgaa acaatagaaa acgatgagag
     6421 gagatgacta ttttgattaa taatgttgta ttagttggac gtctgacgaa agatccagat
     6481 ttgcgttaca cagcaagtgg tacggcagtc gcaacattta ctttggctgt gaatcgtaat
     6541 tttacaaacc aaagcggcaa tcgcgaggct gattttatca actgtgtgat ttggcgcaaa
     6601 tcagcagaaa ctttagcaaa ctatgctcgt aaaggaacac ttttaggtgt tactggacgt
     6661 atccaaactc gttcttatga taatcagcaa ggacaacgtg tatacgtgac agaagtagta
     6721 gctgacaact tccaattgct ggaaagtcgt tctgcatctg aaaatcgcca acaaagcggc
     6781 ggattccaaa gttctggtca atcagctggc ggatttggcg gaaacaataa ctctaatcaa
     6841 acatctcaat catccaacgg tatgcctgat ttcgaccgtg atacttctga cccattcggc
     6901 tcaagttcaa cgatcgacat ttcagacgat gatttaccat tctaaacgaa tttgatagga
     6961 gggaaataga atggcacaac aaagaagagg cggacgtaaa cgtcgtaaag tagactacat
     7021 tgctgctaac catattgaat atatcgatta taaagatatc gaattactaa aaagatttat
     7081 ttcagaacgc ggtaaaatct tgccacgtcg tgtaactggt acaggcgcta aaaaccaacg
     7141 taaaattact gttgcaatca aacgcgctcg tatcatgggc ttgctacctt tcgttagcga
     7201 agactaataa atcctaggaa ttgaatgact gatccttttg atcagctatt caattccttt
     7261 ttttctgtaa aaataacctc tattcgttta gtcgctcgaa tgacgaaagt tatggtaaaa
     7321 tcaaattagt catatatggg aggaaaatta tggaaaataa aaacaatcat ttatccccga
     7381 aacctctctt ccttatcctg tttatattaa tcgaagtatt ggtagtctta attttaccat
     7441 ttcgttttgt tagcgcttcg gtcattctat tgatgaattt atggggtgtt cgctattggt
     7501 tgaaaatgaa acgttggctt attttaaata aacgtgaaaa aatccagcag gcttcgactg
     7561 tagctgaaac caatctagct tatgtctcag aagtcatgcc tgtaggagtg attgcttatc
     7621 agccatcaag ccaaaaaatc gaatggctca atccattagc attgaaaatc aaagatcaaa
     7681 gtggattaac agaggaagaa ttgctagata ctttttttac tgcaaaagaa aaaaagaaaa
     7741 accaagtcta tctagcagat aaaacatttc agtttgatat ggatacagta aagggtgttg
     7801 tttattttaa tgatattacc caaaaccagt tgttaaaact ccatcagacg aacagtcagc
     7861 cggtgattgg tattttatct attgataact atgcagatgc cattgataaa ttagatgaca
     7921 aagagatttc ttatttgaac tctttcgtca ctacactagt ttctgactgg atgaatgaat
     7981 accatgtctt tttcaaaaga atcaacgcag aacggttttt cttcgtagca cagatgatcg
     8041 atctgaaaaa gatgatggat aaaaaatttg atttgctgga tcgctttcga gcagaagctg
     8101 aaaaacagag ctcgcctatc acccttagta tggggattgc ttatggctca agaaccctcg
     8161 atgaaatcgg tggagaagct cagaataatt tagatgttgc tttagtccgt ggaggcgacc
     8221 aagttgtcgt gaaagaggta aaagaaggag caaaacctgt ttattatggg gggaaatctg
     8281 ctaagaatgt caaacgcacg cgtgttcgtt ctcgagcgat gagtaccgct ttgcgtcgga
     8341 tttttgatga aacaggcgat gtgtttatca tggggcacaa gtttccggat atggatgctt
     8401 taggctctgc ttttggtgtc agttgtctgg cacgcttcaa ccagaaaaaa gcttacatcg
     8461 ttatcaatga gaacgagatc attccagatg tagaacggtg tcttgaagaa atccataagc
     8521 atcctgaatt agaaatgcaa ctaatctctc cagaacgtgc catagagctg aaaaaagaag
     8581 aggacctcct cgtgatggtg gattaccata aaccttctct atcgatctct cagggcctat
     8641 atgagcaatt tgataagatt gtgatcattg atcaccatcg tcgtggggaa gagtttccaa
     8701 gtaaacctct gctgacttat attgaatctt cagcttcttc agcttcagag ctagtttctg
     8761 agctgatcca ataccaaagc agcaaaacaa atcgcttaga tcgttttgag gccacattat
     8821 tgcttgcagg gatggttgta gatacaaata gtttcaatgt ccgtacaact gcacgaactt
     8881 ttgacgttgc cagctattta aggtcttgtg gagcagacgc atccttagta caatatttat
     8941 tgagctcgga tttagcgaca tacttggaga tcagcaattt gattgctgga agcgactatg
     9001 tgacttctga tattgttgtg gcggcaggga cagacgaaaa agagtataat agtgttatag
     9061 cagcaaaaac agcagataca ttgctttcga tgatcaatat caatgcagct ttcgtcatta
     9121 caaagcggac agacggcttg attgggatca gtgccagaag taccggcacg atcaacgtcc
     9181 aaatcatcat ggaatcctta ggtggcggag gacacttcac aaacgcagct actcaactgg
     9241 aaaatacgac agttgaggaa acaaaacaga agctatttgc tgcaatcaaa gaaaatatga
     9301 atcaaatata tgataaggag taatccaaat gaaagtaatt tttttacaag atgtaaaagg
     9361 aaaaggtaaa aaaggggaca tcaaagaagt tccagcaggt tatgcacaaa attatctaat
     9421 caaaaataac ttggcaaaag aagcaacgaa aagcagtatc agtcagttag caggacaaaa
     9481 gaaagcacaa gaaaaacacg aagctgaaat cttggaagaa gctaaaaagt taaaagaatt
     9541 cttggaaaaa gaagatactg tagtcgaatt gaaggcgaaa gcaggggaag acagccgttt
     9601 gtttggttcg atcccttcaa aacaaattgc tgaagggtta aacaaacaat acaaaatcaa
     9661 attagataaa cgaaaaatcg aattagaaaa tccgatccgt tcactaggct acacaaacgt
     9721 acctgttaag cttcatcatg aggtgacggc tacgatcaaa gttcatgtcg tacaagaata
     9781 acaaatcttg atagcgatgt tcagctggta agtataacca gaaggaaaaa agacaaaccg
     9841 aaatttatct gagaaaacag ctgcagcaat gaaagcaaaa gctgcaaatc gatttcagat
     9901 tgatttcggt ttcttttttg ggaacagcaa aatagcataa agaaaacgag tataataaaa
     9961 gagaactatt tttttattac actttcggta cgaatgatga cttttctctc attttcttgt
    10021 aaaatgaagc aattgaagac aaggagcgat acgtatgaat gaagtgtggc aggatcgaat
    10081 cccaccgcaa aatattgaag ctgaacaagc ggttttaggc tctgtttttc ttgacgcaga
    10141 aacgatcatt gatgccatgg agtacataga gccaaaagac ttttatcgtc gtggccatca
    10201 aattattttt gagacaatga tcgagttaaa tgatcgtaat gaagcgattg acgtggttac
    10261 tgtcaaagac cggctggaac aagccaattt gttagaagat gccggtggtt taagttatct
    10321 ctcagacttg gcattagcag ttccgacagc agcaaatatc gtctactatg cgaaaatcgt
    10381 tgaagaaaaa tcattgctgc gtacattgat ccagacagca acaggaatcg ttacgaaagg
    10441 attcgagcaa ggagaagaag ttcaaacaat tttagatgat gccgaacgca gtatcttgga
    10501 ggtttcggaa aaaagaaatc gaagcggctt tttatctatt gctgaagtac tgaactccac
    10561 gattgaaaat atcgatcgac tagctcaaaa caatgaagaa atcacaggtc taccaactgg
    10621 ctatcaaggt ctggataaaa tgacagcagg tttccagccc gaagaactaa tcattcttgc
    10681 tgcacgtcca gcggtaggta aaacagcgtt tgcattgaat attgctcaaa atgtaggaac
    10741 aaaaacagat cgttctgttg cgatatttag tttggaaatg ggggcagaat ctcttgtaaa
    10801 tcggatgctt tgtgcagaag gttcgattga ggccagccat ttacgtacag gtcagctttc
    10861 ggaagaagaa tggtcgaatc tgatcgttgc gatggggagt ctttctcgag caaatatttt
    10921 tatcgatgat acaccaggga tcaaaatctc tgaaatccgt gcgaaatgtc ggaaactagc
    10981 gcaagaaaaa ggcaatctag gcttgatatt gatcgattat ttgcagttga ttgaaggtaa
    11041 cggtaaagag aaccgtcaac aagaagtatc agatatctct cgccaactga aaaaactggc
    11101 gaaagagctg aaagtacctg tcattgctct ttcacagtta tctcgtggag tagaacagcg
    11161 acaagacaaa cgaccagtgc taagtgatat tcgtgaatcc ggatcaattg aacaagatgc
    11221 ggacatcgta gccttcttgt atcgagatga ttattatgaa agagaaggcg gagatgacga
    11281 tgatggaaac gagccaaaaa atgacaacgt catcgaagtc atcatcgaga agaaccgaag
    11341 cggtgcacga ggaacagttg aactactgtt tatcaaagaa tacaataagt tttcgtcgct
    11401 ttcgccaaga gaagaatttt aaagaagagg ttgtgagcct tgagcaatcc cgagagctct
    11461 cgtaagaaga tgcaaggagt aaccgcagga acgactagag tacttaattt aaacagatct
    11521 ccaaagggat tgtgacaaaa gtcttgtcac agtccctttt tccgaataaa cggtgttcaa
    11581 tcagctgacc cttggtatta gctcagcaaa cgtcaaaata tgaggaacta ttttgacgtt
    11641 tgtttctcta atactcaggt caaaacgctg atcttacgac ctcttttcct taaacggtgt
    11701 tctcctgcca actcctcggt cacaagtcac aatatcatca aacatgagaa gcagtttttt
    11761 gaaattgctc cttgtgactc agagttaaac ggcaggctca caacctcttt cttaaacggt
    11821 gttcaaaagc ttgcatctgc ggtaatacgc ccaatcaaac gaaaaatatg agaagctatt
    11881 tttgtttggc tgttcttatt acttgatgca ggacagcttt ttcacaacct ctgattcacg
    11941 gtgttccaaa agtagcttct tcgaaaaata agtcgaaatt tcacaaaaat ttgagaagca
    12001 attttcgtac attccttctt atttcttgaa gctgaccact tctgttacaa cctctttttt
    12061 aataaccatt ctaaaaagat aaagttcgtt ttttaagcgt ttttataaaa taaaaataaa
    12121 aatcattcga ttttatttga aatatgtatc tattattgat acaatggttc aggattaaat
    12181 aatatagaac gaggtgttca catgtcatct gtagtagtag taggtacgca atggggcgat
    12241 gaagggaaag gaaaaatcac agactttctc agtgaaaatg ctgaagtgat tgcacggtac
    12301 caaggtggcg acaatgcagg gcacacaatt aagtttaacg gtgtgactta taaactgcat
    12361 ttgattcctt ctgggatttt ttataaagac aagatcagtg tgatcggaaa cggcgttgta
    12421 gtcaatccta aatcattggt taaagaatta gcttatttac atgaaaaagg tgtagaaaca
    12481 agtaatttgc gtatttctga ccgcgcgcac gtaatcttgc cttatcatat caaattggac
    12541 caattacaag aagatgctaa gggcgaaaat aagattggta caacgattaa aggaatcgga
    12601 cctgcctata tggataaagc agctcgtgta ggtatccgta ttgcagactt attagataaa
    12661 gaaatttttg aagaacgtct tcgtttgaat ctagatgaca aaaaccgtca atttgtaaaa
    12721 atgtttgatt cagaaccact tgattttgac gatatttttg aagaatatta tgaatacgga
    12781 caacaaatca aacaatacgt cacagatact tccgttatct tgaatgatgc tttggatgca
    12841 ggcaaacgcg tgttgtttga aggcgcacaa ggtgtaatgc tggatatcga ccagggaact
    12901 tatccttttg ttacttcttc taacccagta gccggtgggg taacaatcgg aagcggtgta
    12961 gggccttcaa aaatcaataa agttgtcggc gtctgcaaag cctatacgtc acgtgtcggt
    13021 gatggaccat ttccaactga attatttgat gaaacaggag aacaaatccg cgaagtagga
    13081 cgagaatacg gtacgacaac tggtcgtccg cgtcgtgtag gctggtttga ttctgtagtt
    13141 atgcgccatt ctaaacgagt ttcaggtatt accaacctat ctttaaactc gatcgatgta
    13201 ttaagtggat tggaaacagt aaaaatttgt acggcttatg aattagatgg cgaactaatc
    13261 taccactatc cagcaagctt gaaagagttg aaccgctgca aaccaatcta tgaagaattg
    13321 ccaggctggt cagaagacat tacaggatgc aaaacacttg cagaactgcc agaaaacgca
    13381 cgtaactatg tacgtcgtat ctcagagctt gttggtgtac gtatctcaac attttctgtc
    13441 ggacctgata gaacacaaac aaacatctta gaaagcgtct gggcgcaaat ctaaggataa
    13501 aaacgacgac tgaatcagga cgttcttacc agagcgagtc gaagtgttta tctatacgat
    13561 aataataact tgaagaggta gtgaaagaga cgtttagcct tattgccgat aggctgtctc
    13621 ttgaacactg tttattcaga ataagggact gtgacaaaaa ccgtgtcaca gtcccttttt
    13681 ccgaa
//
LOCUS       DAZKOB010000070        13645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000070 DAZKOB010000000
VERSION     DAZKOB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.070"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..461
                     /locus_tag="ACG8M6_001995"
     CDS             123..461
                     /locus_tag="ACG8M6_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457649.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(666..1228)
                     /locus_tag="ACG8M6_001996"
                     /pseudo
     CDS             complement(666..1228)
                     /locus_tag="ACG8M6_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(1960..2541)
                     /locus_tag="ACG8M6_001997"
     CDS             complement(1960..2541)
                     /locus_tag="ACG8M6_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0457650.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2724..3350)
                     /locus_tag="ACG8M6_001998"
     CDS             complement(2724..3350)
                     /locus_tag="ACG8M6_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0457651.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3372..4703)
                     /locus_tag="ACG8M6_001999"
     CDS             complement(3372..4703)
                     /locus_tag="ACG8M6_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0457652.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4858..5259)
                     /locus_tag="ACG8M6_002000"
     CDS             complement(4858..5259)
                     /locus_tag="ACG8M6_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0457653.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5345..5551)
                     /locus_tag="ACG8M6_002001"
     CDS             complement(5345..5551)
                     /locus_tag="ACG8M6_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457654.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5646..7148)
                     /locus_tag="ACG8M6_002002"
     CDS             complement(5646..7148)
                     /locus_tag="ACG8M6_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0457655.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7166..7621)
                     /gene="argR"
                     /locus_tag="ACG8M6_002003"
     CDS             complement(7166..7621)
                     /gene="argR"
                     /locus_tag="ACG8M6_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0457656.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7719..8336)
                     /locus_tag="ACG8M6_002004"
     CDS             complement(7719..8336)
                     /locus_tag="ACG8M6_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0457657.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8557..9930
                     /locus_tag="ACG8M6_002005"
     CDS             8557..9930
                     /locus_tag="ACG8M6_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0457658.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10005..11117
                     /locus_tag="ACG8M6_002006"
     CDS             10005..11117
                     /locus_tag="ACG8M6_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0457659.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11189..12826)
                     /locus_tag="ACG8M6_002007"
     CDS             complement(11189..12826)
                     /locus_tag="ACG8M6_002007"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0457660.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(12994..13602)
                     /locus_tag="ACG8M6_002008"
     CDS             complement(12994..13602)
                     /locus_tag="ACG8M6_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0457661.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3670 a         2878 c         2448 g         4649 t
ORIGIN      
        1 aagaaacatt gaacctatgg atccaaaagc tacccaaata ctatagctaa ccccccgcta
       61 ccttcaattt tgcagataaa acgctttggg agaaaatttc aaagacaaca ggccatccta
      121 tcatgcccga ttataaacaa aaagctggct attccttttg ctctgacaaa tttcaattgg
      181 gaaagttcac gaaactagca ggtgaagaag tttcaactgt tcggatcaaa gaatgcccgc
      241 ttcaaataga aacattggtt caacgaattt caactagaca agactttgct attgtagaac
      301 gtgagatcgt agctattttt gtagaagaag ggattttaca tgatccaact catatcaatg
      361 tggataaatg gcaacctctt atctataaat tcagagaata tacaactgcc aaccagtcgt
      421 taactttgaa ttttagattc caagaattca aagagtcata atgataaaca tcgttaggta
      481 taaataaaaa agactcgcag cacgagtaaa aaacaaatag aagctataca ttagaaaggt
      541 cgactgtgac cctatagatg cagtatatgc agcttatgag catttcttct gttctctttg
      601 tttttgggat ctgtgctgaa gtctttttcg ttttttgccg ctctttcttt gaaaaatcga
      661 ttatgttata ccttctccat tactaattca ggcatctgct gagtaatgat ttttcgcgca
      721 aaagaaatat ctgttaccca aggttcgatt tgttcttcag tcaagacaag cggcattcgg
      781 tcatggattg gtgcgactgt gcggttaggt tcagtagtca ttatgattga ttctgccatt
      841 ttttcatcct gatggctacg ataaaaaccg ccaatataca ctattttttc cttatttgaa
      901 aacataaatt tgtctttttc ttcattccac tcattgggaa aacacaacga ttttgcaaaa
      961 atggttgatg aaaggtcttt ttttcttcta ctgtctctgc acgggcatta atcatcaatt
     1021 gtcctttttt aaatccggta aatccccact tcatgatttc cggagtgagg atgttttctt
     1081 cctcttttct taacgtcaca acaagatttg acggaaaaac ttctccagct gctaattctg
     1141 ctgcctgctt cggttgtttc gtctgggctt ctcgataata attctgaagg agctctgact
     1201 ctagatcaaa aaaataacga ccacacatcc taatccctcc caatcttttt ttctatcata
     1261 cactgcaaca agaagttcgc accaacaaaa cgctttcaat ttttgtagaa ctaaaaaagt
     1321 tgtgacaagt gacaaactct ccagtgaatg gtattctcct gccaactcct cggtcacaag
     1381 tcacaatatt caacaaacat gagaagctgc tttttgaaat tgttccttgt gactcggagt
     1441 gaaacggcag gcttacgacc tctgggtaaa cggtgttcaa aagccagttt ctcggtatta
     1501 agccaaattt atgcaaaaat gtagaaagct attttcacaa aatttttctt aatactcgaa
     1561 actaagcgac tttttcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt
     1621 aataagccca accaaacgaa aaatatgagc agctattttt atttggctgt tcttattact
     1681 tgatgcaggg cagctttttc acaacctctc cactaaacgg tgttcaatca gctaaccttc
     1741 ggtattagct caaaaaacgg caaaatatga ggaactattt tgccgtttgt ttctctaata
     1801 ctcaggtcaa agcgctgatt tcacaacctc taatacacgg tgttccagaa gtagcttctt
     1861 cgaaaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
     1921 tttcttgaag ctgaccactt ctgtcacaac ctctaattat taaagaacct tttgcgaagt
     1981 taacaatctt tctttgatca attgaaaatt ctgcctaagt agtgatagat cctgtgtgac
     2041 tttcaacaat aagatacttc cctggatttc agcaatcaac gctttggctt gacggtctgc
     2101 ttcttcatcg gacagttttt cttcagaaag tgccaggata ttttgtttcg tatacttgac
     2161 ccattgattg actaattcta gtagcaacag acggtattct tcatccactg tactcaactc
     2221 aataatcaaa ttacctacag gacagccatg ataagtgatc tgtgtctcat gatcagcgaa
     2281 aatccagtcc aacatttctt ctaatgcttc ttctggctgt tttccagcat ttaagatgtc
     2341 ttcgaacaat tctgtctgcc atacgtccat atgcgcatgg atcaccgcta gacaaacttc
     2401 tttttttgaa gaaaagtaat aataaaattg accttttcct gtatcagaag ctgctagaat
     2461 atctcggatt gacgtttcat gatagccttt tttataaata agatctttgg tcgcttccaa
     2521 gatcttttcc tttgttttca atgatattct cctttttcat ttctcatagt cctatattat
     2581 acagtaaatt catcattttt tcttcttaat cgtttaaaaa agactgtgcc aaattatctg
     2641 acacagccct cactataaaa ctgttctcat atagctagat gttcattgat tcaagaactg
     2701 cgaggacaaa agcaattcct atcttatgat agttgctcta atacttcctc tgttgtcatc
     2761 acttgatctg caataaagcc aaagttcgtc aacgctgcat ccattgcttc ttggccagga
     2821 gcaccaattg catctgtcgc taccacaaca ttgaatccac tctctaccaa ttcacgcata
     2881 tgggaatcgc tacacaagtt tccgttcatt ccaccaagaa ttactgtgtc gattccattt
     2941 tttctcaatt ggagcgccaa atcattgctt tctggtccaa agattttatg aggagagctg
     3001 atgatcgtat tttcatccaa ataaggtttc aattcttcga taaaatcggc tcctgaacct
     3061 tcttccactg attcatattg actttttcta gcaaacatct gctgctcaag catcatatgt
     3121 tctcctgcac cgccaaattg ccatttatgg tcatgtgggt aaaaataatg aggagaaata
     3181 aagagtttgt atcctttttc ttttgcttgt tttaccattt caatcatatt ttcgatcgta
     3241 ttgtattttt ctaagatatc ttttgtcaaa ccaaagccac cgcctgctgg tttcaagaac
     3301 tcgacttgcg ggtcagtgag aactactgct gtatttttat tattgaacat ggtggattct
     3361 ccattcgtta tttattttaa tacatcattt agagcttcac tcattgtcgg atgagaatag
     3421 atttgatctc tcaataacgt ataaggaagt tttccattca tagctagcga aatcaagttg
     3481 atgacttcat gagaatcttc cgcatagata cttgccccta gaatcaagtc agtttcaggg
     3541 tcgactaaaa ctttgaatac acctttagga tcttctaata cttgtgcttt agggacaccg
     3601 cttgtcatat acttgaataa tttgtaattg acgccttgtg ctttggcttg tttttctgtt
     3661 aaacctacat tggataaagt cggagtgata aatacactgt aaggcacagc tttccggtta
     3721 ttcgttgttc gtttattctc gcccttcaac tgatccataa tgattcgata atcatctaga
     3781 gagatataag tgaactgcaa tccgcctttg acgtctccaa tcgcccaaat gttgtctgca
     3841 tctgtacgaa ggtattcatc cacagcgatt gctccgcgat ctgttagttt aacatctgta
     3901 ttttctaatc caagtgatgc tgtacttggt tttcttccag ttgcagccaa aacttttgac
     3961 gcttggattg ttacttcttg gccatcttct gtaaaggtga cggcagctgt tgattcttgg
     4021 tctgctactt gatctacaga tacacctaga tggaattcga ttcccgcatt tgtcatgtct
     4081 tctaaaatca tttgcgcgat atcttcatct tcacgtggaa gaaattcaga atgggcatca
     4141 agaacgacaa ctttagatcc gtattcattg aacattgatg caaattccaa gccaatatat
     4201 ccagccccga tgatcactaa tttttccggt aattgttcct gatccatcgc ttgcgtagaa
     4261 tcaatcaagt agcgacttgt attcaatcct ttgattggta aaatgattgg ttcagaacca
     4321 gtattgatga aaatccgttc gccttcaaca atcacttttt caccattatt cgtcacttcg
     4381 atttgatggt tagaaagaaa acgtgctgta ccgtccatca ctgtcccagt tgcctcatcg
     4441 ctgatcatat gataattttt ggcgcgaagc atgcccgtca atttttcttt ccgagcaaca
     4501 gcctctgtaa agtcgattcc tctttctcca ttgaaaatca atgatttaga aggaatacag
     4561 ccgatgttga tacatgttcc gccatacata cgtgttgact tttcaacgac taaaaccgat
     4621 tcaccttttg aagctaacgc cttcgctaaa gtcttaccgc cctttccaaa accgatcaca
     4681 atattttgat atttttccat catttttccc tcgcttcttt tttacctacc gattggtaca
     4741 aaaatatgat aaccgataat tgtctattct ccaaatcatc tgctttattt gtcaaaaaaa
     4801 accagacaaa aagcggtcct ttcacgtttt tagtctggtt cgatttcctt tattcttcta
     4861 aacaatcgtt acttctacat cgatatttcc tctagttgct ttggagtaag ggcagaattc
     4921 atgcgcatct tctgcaattt tttgggcttc ttcttcgctg atgccatcga tatgcacttc
     4981 taatactaca ccgatctgat agccgccatc atctaaatta taaagagaga cacgtgcttt
     5041 tactgtggac tctgctgtca ttcctgcttc tttgatcata tgttgtaccg ccccattgaa
     5101 acaagaagca taacctgctg caaataattg ttctggattt gttccttctg catggattcc
     5161 tggaggagtg attttcatat gcatcgagcc atttggtgcc tcgacttctc cattgcgccc
     5221 gccatggttg atcattgctg tttcgtatgc ttttttcatt gtactgctcc tttcgattga
     5281 ccatttgcta cttggctata attatagtgt atgacaagta aggcgctttc tacaaaaatt
     5341 ctgcttattc gaataccaga atgccgtctt ctactatttt tccaaaaaac aaaaaaatac
     5401 tactcgtgaa caacagcgtc atatttgttc caaaataaat agcaatcttt tctacataat
     5461 cttttatatg attgatttta agatacatat ataccagaaa gttgacccca taaaccaata
     5521 ctgttaaaaa aataaccata ttcgttacca ttttttcctc cttttgctgg tctccctacc
     5581 agcactcgat ttaaccatac cacttttttt gccgtccaag caactagcct ttcaaaaaga
     5641 ctggtttatt ctgctgttaa tgattcaaga tcagaaaaat gtccgaataa ttttgctaat
     5701 tttactaacg ccaacttgtt ctttccgaat tcatcatttt ctatctgtaa tactagtaac
     5761 ggctcatgta agcttaaacg gatcaaaaac cagccgtttc cgtatggctc ttctagatca
     5821 tatcgaatac cttcttcatt ttccgggtcg aagttcatcc ctttttcttc ttccaacagt
     5881 cgcttcattt cttctaatat cttgtttcct tgttcgcgtg gttcatcgct gaacaatttt
     5941 aatcggattt cttgtacttc tacgggctgt ttcaactctt tgatttgttc atctaatgtg
     6001 ataccttttc tttttaattc tggcaaaagc atcaggatct ttgcgatgac atatgcacca
     6061 tcatccagaa aatagttttc cttgaatgca gcgtgtccgc ttgtttcgat ggctagctgt
     6121 gtatctttcc caagacggtt aagttctaat gctttattga ttacatttcg atagccggaa
     6181 atataacgaa cttggatgcc tcctttttct tcaatgaatc tttgtagatg agaagaagta
     6241 ggggaattcg tcacgattct tgttcctgga tgctctttta atacgattgt acttaatact
     6301 gcgatcaaat tatttcgatt gatcactgtt ccagaagcac tgacaactgc agaacgatcc
     6361 acatctgtat cgaaaatgat cccaagatct gcttcatttg ctaaaacagc ctgcttgata
     6421 ctttccatcg cttctttatt atcaggattt gggatatgat taggaaatcg gccatctggt
     6481 tccaaaaatt gggaacctgt tgaatccgca cctaagctat tcaatacttt ttcagcaaag
     6541 aatccaccag caccatttcc ggcatcaaca atgattttcc aaccatttag tggtttgctt
     6601 tctagtgttc caatcccctt tttaattttt gtgactaaat cttgcgcata aggtgtcaaa
     6661 aggtctgctt tttctacgct tccagtttgt tcgcttttta ttttttgctg ctgtgatagg
     6721 ataaaagcaa tatcttcttt ttctgctcct ccttctgagg taaagaattt tattccatta
     6781 taataatagg gaagatggct agcagtcagc ataaccgcag catcgcacga gaactgttca
     6841 aattgagtgg acataaacat agcaggcgta gtagctaatt cgaaatcgat gacatgtatc
     6901 ccttgttctt tcagactttg gatcaacgcc tctttcagtg ctggtccgct tatcctactg
     6961 tcatgaccaa tcccaatcgt ccatttttta tttggatcgg ttgatttctt ctttttcttt
     7021 atccagtttg ctagtcccca gccgatcttt tttaccatgt ctgtcgtcaa gtttacttca
     7081 tatccttcaa ctggtaatgc gattcctcga atatccgatc cattttgcag cgctttcata
     7141 tctttcatat aaaagtcctc ctcatttatt ccctcatttt ttccagcttt tctgcaaatc
     7201 gctgtgcttc ttcttctgag cggcaaataa taatgaatgt atctacaccg gccatcgttc
     7261 cgacaacttc agggtaagcc acttcatcta gccaattcgt taccacatcg gcaccatttc
     7321 gttcagtcaa tacgataacc atgaactgaa cagactggat acgcaaaacc tcgcgtctaa
     7381 ttgctgtacg caaacgttct tcatttaaag aaacagatgg ctggttaaat aatgtatagc
     7441 gtactatttt gttttcattt tgacttttta ctaacttcat ttctcgaatg tctcgagaaa
     7501 tcgttgcttg agtcacatcg gcgcctgctt catgcagccg cgcaagcagc tcttcctgtg
     7561 tcccaatagg ttgttctgta atgatctggc ggatcagtgc ttgtcgctgt ttctttttca
     7621 tattagacac ctctcaatgt tgattatagc aaaagataag taatatttct tcatattttg
     7681 gtatttttta cttttatttt ttgttttcat tcaggttgct aacctttttc tattttaaca
     7741 cgcaaaatcg tcttcatttt ttcaggtgct gattttcttg gatcgctgat gtaaatctct
     7801 ttgtgttctt tagaagctct ttcatagcct tcttttgcga tgaatgcttc taatttctca
     7861 aacgttgctg gttcttcggc atagggcccg acgtgtaaga tttgggcaca aagaccttct
     7921 tccatctttt tgaatactac ttgttctttc aagtcttccg gaagtttgct agaacgctga
     7981 atggcttctt gtgctatcgc ttctgtaaca aagtcaggct gtcggatcat caattgataa
     8041 gaaaaatgtt cttttttcat cactggttca tgcaaatact tttcttgcag tccccattgt
     8101 ccttccagtg gatagacagt atacatctga taatcaggaa tcagccaatt ttttttaggc
     8161 gacatgcgta ttgcatagct gactgcatat agacaggcta ctcttcgttt aaagtcttcc
     8221 tgattcgggt cgccgatccc tctgatgcta aagaatgatt ttttaggaac ctctagtatt
     8281 tgcgggcttt ttttcatact atagctcttg tctttacgcc attcaatttt ttccattttt
     8341 cttcctccgc gaaattccat ttagttattt attatcatac ctctttttga gtgacttggc
     8401 taatcgtgtt gacactattt ttcagtttgt gctagaataa gtgaagtaaa taaatcgtac
     8461 agataacacg tttcggcgtg tcgagagaga ggcggtttct atttttataa tagaaatcac
     8521 tctctttttt tacgaaattt tttagaggtg atgtaaatga caaaacaaaa acagctgctt
     8581 ttagtacctg cagtaattgc aacagggatg atgtcttttg cgggcgtatt gatcgagacg
     8641 gccatgaatg taactttccc aactcttatc agacagtttg atttaacgac tgcacaagtc
     8701 caatgggtaa caacgattta tcttttgatg atttcgatca ttgttccgct gtctacttat
     8761 ttgaatcgca attttagtct caaaaaactc tttttagctt caaatctgct tttcttggca
     8821 ggtataatga tcgatttctt ctctcctacc ttcatggtcc tattgtttgg tcgattgctc
     8881 caaggtgcag cgacaggtat cgctcttccg ttgatgttcc atattatctt gacatttgcc
     8941 cccctcaatc gacgaggcgc gatgatgggt gtcggtactt tgacaacggc cattgctcca
     9001 gcaattggcc ccacttatgg aggcattatg acttcacatt ttacatggaa tcatatcttc
     9061 ctgtttcttg tgcccgtttt gtttctttct ttgattatcg gctggtttgc tattcccgag
     9121 atggcagtac aaaaaagtgg ttatttggat tggataagcg ttttaggaat ttgtcttttg
     9181 tttagtggaa gtctaacatt tttgagtacg ttgggaacag tttccagctg gattgcttta
     9241 atcatcggcg gtattggctg gttccttttt tactatcgaa gtaaaaaagt ggagcatcca
     9301 ctgatccgac tgtctattct ttcaaataaa acattccgct tgtttttgat tggcttctta
     9361 gtttatcagt ttttattact tggtgtatct tttgtcttgc cgaatttcct tcagatcgtt
     9421 caaggaagta gtccttttgt cgctggatta gcgatgcttc ccggcgcagc ggttggtgct
     9481 ttgctttctc ctttttccgg gaaaatgctg gatcaatatg gtccgaagaa gccaattttt
     9541 gctgggctga ttctttcttt gattggttgg ctagcattat tcctactcat aggacacgct
     9601 tcagtgggtg tattgattgc ttgtcatgtg ttttatatga tcggtatcgg attatcttac
     9661 agcaatatga tgaccaccgg aatgaatgca ctgaatgaag aactacaagg agacgggaac
     9721 gcaatgttca ataccttgca gcagttttct ggcgctgtgg ctacttcatt agtagcggtc
     9781 attatcaatt acgtccagca tcatacttcc cataactatg aagtgtctac tacattagga
     9841 tcaaaagcag cattaggcgt cttgttgctg ttgcttctcg taagctttgc tcgattcgcc
     9901 tattatctgt tctttgctaa aaaagcgtag attccatata cggcaggtgt tgcatctgcc
     9961 gtttttttgt gtgaaaatgg ttgtttgtaa aaggaggctt ttttatggaa aaaattgcca
    10021 ttatcggtgg cgggatcatt ggtatgaccc tagccagtta cttagataca tcaaaattca
    10081 atgttgcttt atttgatgaa gggacaggac aggcaactaa agctagcgct gggattatct
    10141 ccccttggtt gtcgaaacgc agaaacaaac aatggtatcg tctggctaaa gacggcgctg
    10201 cttttttccc taaacttata aaagatttcc aacttgatca aacgatttac gcacaatcag
    10261 ggacactttt gcttcgacct gaaaaagatt tagctgaatt ggctgttctt gccgagaaac
    10321 gaaaagaaga agcgccagaa attggaaaca tcgaatggct ttcacctgaa cagacatctc
    10381 aagcacttcc gttattgaaa ccagcagttt cactgcgtat cagtggtggt ggtcgtctgg
    10441 atggaaaagc atatttagaa catcttggtc agcgtttagc acaaaaacaa atggagcaga
    10501 tccaggcaaa agtcactttg cataagaaga gcgattcttg gctcatcaag ggaccgtttg
    10561 gagaatacac agctgatcgt gtaattttga cacctggccc ggcacttaag cctttattac
    10621 aagaattagg ctatgctgca gatatacgtc cgcaaaaagg gcagctctta gcatttcaga
    10681 catcttataa gatgagtcaa gactggcctg ttgctatgct tgacggagaa gctgatttca
    10741 ttcctttcat ggatggaaag atccttttag gtgctaccca tgaaaacgat caaggctggg
    10801 atttggctcc tactagcgaa gcttataagg gattgactaa gagtacagaa cagttcttga
    10861 ctgatccaga tcagataaaa aactggccat acgagttccg tgtaggtacc cgtgcttata
    10921 cgtcagattt ttccccattt ttcggcgtac tgccagatga tccaagctta ctggctgcta
    10981 gcggtctagg ctcttctggc ttgaccacag gtccttatat cggctatcta ttagccaact
    11041 tcttaaatac tggtcattgg cctacggaag attatcaaaa ggacatctcc acctatatta
    11101 cgaaaaatcc atcatagaaa aacagctttt tcaagtaagg atcccgagcg atattgcgca
    11161 tggattactt cttgaaaaag ctgttatttt agttcaattt ctgaatcaaa ttctccaaac
    11221 tttcttttgc ttcatctggc aagagatctt ttcctatctg agcttcaatg aatcgtaaac
    11281 gaatatcaag tcgttgtttc accagatttt tataggacgt atcctcaaaa gaaacaggaa
    11341 aatctggtat aggtaaatag tggatgtctt caaatggacc aaacgacttg aattctgccg
    11401 tttgttgcac gattctttca acgattgaca atgtcgtttc aggtttgatt tttttatcat
    11461 cgaaatatcc agtattcagg acatagcagg ctgtttgctg tttcaaaaac aactctcgaa
    11521 aagcttgata gtcttcccta agaggataga cacggaaagg attagcaaat ggttcaatga
    11581 ctagctcatc tagctgtctt tttgtagtga tattttccgc gtctgatcgt tttgttgcta
    11641 atgttgcacc aaaagacgct gctaaaactg ggtcattgat cttgataatt ggcggaagcg
    11701 aatcatcttt catgatccaa aaaacagcat cgatcttttc gctaagatga tcgactcggt
    11761 cgggtgtcgc ataacgtgat ttgactgtcc gcccatttcc gtttcgtata tctcctgtta
    11821 ctaggacttt ttctccttta cttgtacatg tcacgcccac attctgacaa gtaaggaaag
    11881 aagaaatggc cggatcatca gacggatagt cttgtgtttt atcaaagtaa gaaggttcca
    11941 aggcagtagc aaaaccattt tttcgagaaa taacgaaagc atcgtcatgc aagatcgaaa
    12001 cagagtgacc ttccgtatgt tttgctaagg taatcgttga ttttccagaa ccggacaacc
    12061 caaatacagc catcgtatag gtctggtcat tactagcata ttgtttcatc ccaccgtggc
    12121 aggcaataaa gccattgcga tttcccaatg cccaagcaag cgtcaaagtc gcttttttta
    12181 attctccaaa gtaacgtaat cctagcacag ccgctgtatt atggagagca tcaaacaaaa
    12241 tgagtccatt tggaaatgtt tcatgtgtcc attcgggatc acaaaagata taaagatctc
    12301 cctcatcaaa ggaaacagac ttttgataag tctctaagat cttcgaattc gctggctgaa
    12361 aattcagcaa ataagaatac aaattgttct caaatccttc tggaatcagc aaatgacttt
    12421 gcacagaaaa ttcttcagat aggccaacga ttacttttgt atggtaaaac tggcgcttgc
    12481 gactttgata cacagcttct cttaaaattt tctcgtattt gctttgttcc ttgtctaagt
    12541 cctcaaagat ccgccgagca cttgctgttc ttcccacaat gcttccatca ttgaatacta
    12601 gtgtcctaac atcagctggt aaatctagtt cttcagcatg tgagattgtt aaatctgtga
    12661 tgatcgttcc tggactccct tttgctagtt gataagctgt cttcctatca aaaacttcat
    12721 gaacattatt accgtaaaat gtcgtttcta caatcgatcg aatactggat aacagtggat
    12781 ttccacttgt gatttcgttt aattcgagac tctcttttgt actcatggct cctcctcatt
    12841 ccctaaacaa aaaaacgttt tctttattat acattattcg agtttttatt tgtactgctg
    12901 cgctgtggga agcaaacgag ttatttttca cacaaaaaaa ccgagaactt gaataaacaa
    12961 gtttctcggt ttcatcattt tccttttatc ggcttagtac cagccgtttg ctaaccagaa
    13021 tgttttagca gcatcccatg aaccataacg agatgttaca tattgttctg ctacacgttc
    13081 ttgattggca gctgaatagt cgccgtttaa gtatgaagca tctaattgat aacggccgat
    13141 gtaacgtccg ttagtagctg tgtatgaacc gcctgattct ttttgtgcga tccattcttt
    13201 tgcagagctt gaagttggcg ctgttgcagc agctggggca gcaggagcta cttcaactgt
    13261 ttcaacaggt gcaacgtatg tatgttcttg cgttgctgtt tcagttgtag atgctgtatt
    13321 ttctgttgtt ttgttttctg ttgttgctga agaatcattt gaagttggga ttgttaattg
    13381 ttcaccaaca taaatgcggt tgatgtttgc gatcgaattt ttttcagcaa ttgcatcgat
    13441 cattgagttg tctcctgcaa atttttgtga gatcttagat aatgaatcac ctgattttac
    13501 tgtataaact tcatcagcgt gagcatttgt agttcctaca aacattgcag tagcaccagc
    13561 agcaaaagtt gtacctaaaa caattttctt aaatgaattc atattaaact cctttttttc
    13621 tccgtttttt attctttcga cgcta
//
LOCUS       DAZKOB010000071        13624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000071 DAZKOB010000000
VERSION     DAZKOB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.071"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..705
                     /locus_tag="ACG8M6_002009"
     CDS             106..705
                     /locus_tag="ACG8M6_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0457662.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
     gene            705..1280
                     /locus_tag="ACG8M6_002010"
     CDS             705..1280
                     /locus_tag="ACG8M6_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0457663.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            1305..4976
                     /gene="brxC"
                     /locus_tag="ACG8M6_002011"
     CDS             1305..4976
                     /gene="brxC"
                     /locus_tag="ACG8M6_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0457664.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            5040..8672
                     /gene="pglX"
                     /locus_tag="ACG8M6_002012"
     CDS             5040..8672
                     /gene="pglX"
                     /locus_tag="ACG8M6_002012"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0457665.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            8788..11310
                     /gene="pglZ"
                     /locus_tag="ACG8M6_002013"
     CDS             8788..11310
                     /gene="pglZ"
                     /locus_tag="ACG8M6_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0457666.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            11428..13461
                     /gene="brxL"
                     /locus_tag="ACG8M6_002014"
     CDS             11428..13461
                     /gene="brxL"
                     /locus_tag="ACG8M6_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0457667.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT         5003 a         1836 c         2641 g         4144 t
ORIGIN      
        1 tataataaat atgtattgta tagtagatac taaaaaatga atgctaacga tgagcaaaaa
       61 acacttgttt acttattgaa ttatagatga ttaggaggca atctattgaa taaaaaatat
      121 tctgctggtc tagtttctaa acgcttttgg ttttcagagt tcaagttata tattcaatta
      181 cgactagaag gatatacaga tatagaaatt aaagagaaaa atgaacatga aaatattttt
      241 tttgcagtga gcagtaaccg tcaaaaagaa atctatcaga acgtgaaaag gagagttagt
      301 caactggatg aagctggatt gcaacttttc aatcagttaa atatagataa tcaaaagtta
      361 gtgaatttga tttccatcct tatgattgat gatcttttta atgaatttat gactgaggta
      421 tatgctgaaa atctacgtaa aggaaatttg caattaacac cagttgatta ccgttctttt
      481 tttacagaga agcggagaag ttcagaagat atttcaaaat ggaaagatta tacgattaaa
      541 cggcttattg ccgcttatca aacgaatcta attgaaacag gattagtcag aggggaaaaa
      601 gaatatggac gaatgactcc gatattatta gataaacgat tgattcaatg gttagttgac
      661 aagaatcgta aagatattgt actagcactg ggaggaattt tataatgtca ttatcgattc
      721 aagagcattt ggaaaagttg aatgaagtaa ttcaaaaaga agatttttta ttgaatcgtg
      781 gtttaggtaa tgaagtaggc tattatatct ttgactacga tccgaaagac gagttaattg
      841 taagagatta tattaatgat atatctggaa catctgttgg tgataattat gaagtgaaag
      901 tttataatct ctatgtgatt atgttagagt atctagaaaa acgcggtttc actgaaaaag
      961 cgattaagat ggaagaacga cgtggtttac aaaaattagt ggaaagcatt ggtcgtttat
     1021 tgaaaatgga tttacctgag gaagaaaatg tattttttga atatgtggat gcccatacac
     1081 cagaaaaatc agttgtgttc ctgacaggaa ttggcgaatt atatccatta attagagcgc
     1141 actctatttt aaataaaatg catcaggtat ttgatcgcgt accagttgtg atgatgtatc
     1201 caggggaata tgatgaatta agtttgacaa ttcttaatac caataaagat gataattatt
     1261 atcgagcatt tagaatttag ttgggatgga aggtaggaga ttaaatgcta attaaagata
     1321 tttttgcaaa accaattgac cgaaatattc aaggagttat taaggtcggt caagcaaagg
     1381 atgaaaatgt tcaacaagaa ttagaagaat acgtggttac gaaagaacta caaaaacact
     1441 ttaaaacaat ttttgatgcc taccaacgga gtatcaatac accaacagat aaaatgggtg
     1501 tttggattca ggggttcttt ggttcaggta aatctcattt cttaaaaatt atttcttact
     1561 tgctcgcaaa tcgagaaata aatgacaaga aagccattga ttacttcaaa gaagatggga
     1621 aaatcaaaga tccactgact ttggcaacga tggaacggtg ctcacaaata aatacagatg
     1681 ttattctgtt taatattgat tcaaaaagtg atgcaggtgg taaaaaagac aaaaacggaa
     1741 tcgtgaatgt ttttttaaag gtatttaacg aaatgcaagg attaagcacg attccacatc
     1801 tagcagattt tgaacgtcgg ttaattaaag aagaaaagtt tgatgcattc aaagctgctt
     1861 ttgaagagat taatggggca aaatggcaag aagagcgcca tgagtttgat tggatccaag
     1921 atgatatcaa agaagcggca cttaaaatcg attttatgag tgaagccgct attgataatt
     1981 ttttagaaaa agcatcagga aattatttaa taagcattga tgactttgca aatctagtta
     2041 aaagctatct tgattccaaa gaagcagacc atcatattgc ctttctagtc gatgaggtcg
     2101 gacaatatat cggcggtaat cgtgagttga ctttgcagtt acaaaccgtt gtggaagatt
     2161 tgggtcgaat ttgtgaaggt aaggcttggg tattagtgac aagtcaggaa gcgattgatc
     2221 acgtaacaaa ctttgataac aattctgata atgatttttc taagatcaag gatcgttttg
     2281 ccacaactgt gagtttatct tcagcaaatg ccgatgaagt aattaaagaa cgtattttga
     2341 agaaaaaaga gtttgccaat gattctttag ccttacaatt tgaaactgtt caaacagaat
     2401 tgaaaaataa attactcttt gttgatagtc ctgaattaaa gctcttccaa gatgctgaaa
     2461 attacgcaga agtttatcca tttattccct atcaattcaa tttattgggg caaattctaa
     2521 ccgagattcg taatcacagc tatcaaggat caaacttatc gtctggtgaa cgttcattac
     2581 tagcaatgtt taaagaagcc gctgaacgaa ataaggaaaa tgatacaacc agtttggttt
     2641 ctttagatca gttttattac tcgttagaac gttggattga tacagcaatt agccgagtga
     2701 ttacacaagc tacaaataat tctcatattc aaagaccaac tgatatagat aactttaacg
     2761 taaatgtctt acgaatttta ttcatgatta aatatgttga tcaagccatc aaaccaaatg
     2821 ttgaaaatat tacgagcttg atgataagaa atatcaacga agaccgcctg gaaactaaaa
     2881 aacgagtaga agaagcattg gaccttttaa ttaaggaaaa ctttgtgcgg aagaacttag
     2941 atagttatat tttccaaact gatgaagaac aggaaattac tcgtgagatt aaaaatcaaa
     3001 atattacaga tagtgatttg atcgtagaac tatctaaacg aatttttgac ggcgttctag
     3061 accgaaatag ttttgcctat gaaaaattag gcatgtcagg tggaaataaa tttaaaaacc
     3121 ggtatacgtt tacttatgca caagtgttag atcaacgtcc atacaagcaa gctggaaatg
     3181 ctgaaatgac agttaatttt atttcaccat actctgattt agcaggtcaa gaggcagaac
     3241 ttgcgcttaa gagttcccgt aacttattat tcgtagaatt gccatcagat aaagaattta
     3301 aagatgatat agcaatgtat gctagaatga atctttattt aacaacgact tattcgagtg
     3361 tagtaaagaa ctttgacgcg attcgttcgg aaaaatctgg tgaattacag gaactaagtg
     3421 caagtatcaa cgatcaatta aatgaactgt tgaaatctgc aaaatttttc tataacggtg
     3481 cagaaattca actgacaggc tccgacttta aagcaaaatt ccaatcagca cttcagaaaa
     3541 tggctgaaga agtataccat aaattaaacc aaattgatgc gcctaaagaa aaagaagata
     3601 tcttatattt agtgaaagaa agcaaaaatg aaattattga agatccatca aatgagcaag
     3661 cagttcagga aattttcaat tttatcaatg atcaaaagag aaatcaccta acgattacga
     3721 tgaaaacaat tcgtgatcgt tttgaaacaa aacaaccgta tggattcaca gaacttgatg
     3781 tggaatggtg catcgctaaa cttttcatgt ctggtaaaat tgatttactt tataacaatg
     3841 tacaaatttc acggatggat gatccaaata gtattgtaat gattattcaa aaacgacgag
     3901 aaagtgaaaa gataaaaatt gaaccaagcg cggtgatttc gccaaaagca aaaaaattgg
     3961 ttgacgaaat cggtagagaa ctgtttcaaa gtcgtaatat tgttgatgaa aataacaatg
     4021 aacatacaat ggaaaatttc aagaaatatt taaaaggtca attagacaat ttacgctctt
     4081 attttgattt acaaaacact tatacctacc ctagtgctta tgaatatgag caaataaaaa
     4141 aatatcttga agtaattgtt caaacgtcag atgttgaaga ctttttttat acaatcaaaa
     4201 aatatcgcga tgaattattc gactgggctg aaacatatga taaaattaaa aatttccaca
     4261 agcaaaatag tactcagaaa aaaatctggg agaatgcaca agataaaatt agattaatcg
     4321 aaaaaagtcg gcgtcaaact tctagccaag aaattgatca attgattcat gagatgaaaa
     4381 ccattttgaa aaaggctgat ccttattcag atatgatgaa actaaatgag ttgattcaag
     4441 aatttattga acaatataat caactgttag agttggaaag tcaggaaata ttaaataaat
     4501 taacggaaga aaaacaagca acatttaaat tattagaagg aaaagatttt aaggatcaat
     4561 ttgtaccaat agttgaccaa gaatttcaag aactgaaagc aaaaattgaa caagcgaata
     4621 ctatagatga tcttgttttc ttatcattaa acgtagcaga acagtatttg atctttaatc
     4681 aacgtttcga taatgaagaa aaccgcagac gtcaagaaga acaagcacgt aaacaaattg
     4741 aagcagcaaa cttgattcgt gaaactgaaa aagatgaaat agactctgca gcaaaagctg
     4801 aagaaatagc gatagtaaaa cctactattc aacagaaaac agtaccaatg agaacactta
     4861 acgttcatgt taaaactatt aagtcagcag cagatattga tgagttaaca gctgaaatta
     4921 atcaaaaact gaaggaacaa cttcaagaaa atacagaaat cacgattcaa ttctagtgaa
     4981 aagacaggca taaaaaggtg cctgtctttt caaaggttac atatagaagg gaaagaaaaa
     5041 tggataagaa agcgataaag aatttcgcgg tcaatgcccg tagaaaatta attgatgaag
     5101 taatgactaa agcaaaacgt ttaggaattg atgaatcaaa aattgaagat gtgaagaaaa
     5161 ttgatagcaa tttacaagaa atcgaaaaat caggaataag aattcaagga aaagaaatag
     5221 cgcaaagaaa caaattgatt gcagaactaa aacaacgtgc ttctgtcact tcttattcag
     5281 aagcttttca agagttaatc gaagaggttg cttatacgtg gtttaaccga ttgattgcca
     5341 ttcggtttat ggaggtaaat gattatttac ctgacacgta tcgtgtatta tctagtgaaa
     5401 taagtgggaa aattgagcca gatattatta cagatattta tgatgccgat atctttgaag
     5461 aattatctag taatgaacag caacaagtca gaacttggaa aacagataat tcagcagaag
     5521 caatggacaa gctgtatcaa ttgattttta taaaaatctg taatcaattg aatgattact
     5581 tacctgagct atttgaagaa gtagaagatt atacagaact tttgtttaca gcttcttata
     5641 ttaaaaaaga tggtgtaatc gctgatcttt taagtattcc agaggatgat ttctatgttc
     5701 aagctggtgg acaggtagag attattgggt ggttgtatca gtactataat agtgagccac
     5761 ataatcaagt ggtgaatatt tataaaggga ttgtcaaaaa acaagatatt ccggcagcaa
     5821 ctcaactatt taccacagat tgggtcgttc gttatatggt tgacaatagt ttaggaaagt
     5881 attggttaga tcattatccc aatagtgaat taagaaataa gttgaaatac ttaattccag
     5941 ataaatatgc ttttgaacaa gcaacatctg taagtaattt aaaaccagaa gaactaaaaa
     6001 ttatcgataa cgcaatgggg tctgggcata ttttagttta cgccttcgat gtattgatgg
     6061 aaatttatct tacgttagga tattctgagc gtgatgctgc acgtgcaatc gttgaataca
     6121 atttatatgg tcttgaaatc gataaaagag cgtatcaatt gtcttatttt gcaattatga
     6181 tgaaagggcg tcaatacgat cgtctttttt tgaaaaaaag tctatcgccg aatttaagag
     6241 agtttattga ttctcctaaa gtgacagacg agtatcaaga gcgtattagt gaattgaaca
     6301 atgataagta tagtgaacgc tggaatgaat acaaagagtt aatttttgat caatttgaaa
     6361 atgggaaaga acttggatca atcattaagt tagtcgataa aaataatcag gcaataacta
     6421 aagaatactt aggaaaagta agagagtata tatcaaaatt caacgaattt acgaatatgg
     6481 atattcttta tgggatgaag gaaatacaag aaaaatactt gcacattctt gacgtggctg
     6541 aaatgctagt tgataaatat gtagcggtag tcactaatcc gccttatctt aataaaatgg
     6601 ataagaaatt aaaaaaatat gtaaatgata actattctga tgtcaaaaaa gatctatttt
     6661 ctgtgtttat taaattgaat agccaaatgc tgattgataa tggatatgct tcctttatga
     6721 cgccatttgt ttggatgttc atttctagct atgaaaagtt acgctcattt ttaattaatg
     6781 aaagaaacat ttctagtttg attcaaatgg agtattcagc ttttgaagaa gcaaccgttc
     6841 caatttgtac gtttacgata aaaaattcaa atgaacaaga tggaagctat ataaaattat
     6901 cagattttaa aggtggaatg gaagttcaaa aaattaaaac gttagaagca attcatcagc
     6961 atggtgaatg cgattatttt tatagtacta atcaagacaa cttcaaaaaa atcccaggaa
     7021 tgccgattgc ttactgggca agcgataacg taatcactga ttttgctcaa ggaaaattaa
     7081 ctaacgatgt tgcagatgca aaacaagggt tggcaaccgg aaataacaat aaatttttgc
     7141 ggtattggtt tgaagtagaa tttaacaaaa taaagtttga tgctaaaaca ttagaggacg
     7201 ctaaaaagtc tagaaaaaaa tggtttccta caactaaagg tggtcgtttt agaaaatggt
     7261 atggaaacaa tgaatacgtg gttaactggg agaatgatgg agatgaaata aaaaattatg
     7321 ttaatgaaaa tgggaagtta aaatctagac cacaaaatct taattactat tttaaaaagg
     7381 gaattacttg gtcgacactt tcatctggac aagtatcttt tagattgcag aatcaggcaa
     7441 tttttgaaac taaaggatct gttctgttct caaaggatat aactgacttt gaagaaatat
     7501 ttggctatgt aaactctaaa ttagttaatc actttttaac ttttatttct cccaccttgg
     7561 attaccatga aggaccaatg ggaaaactac cttttcttaa agttacatct gagagaaaat
     7621 taaaaaaaat aagagaaaaa gtggatgaaa atatcgatat agctagaaaa gactataatg
     7681 ctcttgaaac gtcatacgag tttagaagaa acattttgtt aagttatatg tttaaaaaag
     7741 atctctcaga ggcatttaac tgtctaagta ggcagttggt taatcaattt aatactctaa
     7801 aatcgaatga agaggaactg aacgacatat tcattaatca ttattctctt gaaaaagaat
     7861 taacaaaaga ggttcaagat gaatatatct ctgcaaggtt acctgataag gcgagggaaa
     7921 ttaaggattt tatttcttat tcaataggtt gtatcttcgg tcgttactcc ctagacgaag
     7981 atggtctcat cttcgctggc ggcgaatggg atccttcccg ctatcaaaaa tttaaacctg
     8041 atactgataa tgttttactc atcaccgatg atgagtattt caaggatagc caaattgaca
     8101 ttgtcaatcg tttcattgat ttcgttgaga ttacttttgg taaagaaacc cttgaagaaa
     8161 atctccagtt tatcgctgac actttaggtg gcaaagggac aagtcgagaa attattcgtc
     8221 gttacttcgt acaagatttt tacaaagacc atttaaaaac atactccaat cgaccaattt
     8281 attggcaatt ggactcagga aaacaaaatg gctttaaagc gttaatgtac ttgcatcgct
     8341 atacaccaga tcaattagga aaagtacgta ctgaatacct acacgaacta caaaaagcgt
     8401 atgacaatcg aatcgaattg cgtcagcaac aaatttcaac aagtgataac ccaaaagaaa
     8461 gaaatgcaat gacaaaagaa attgtaaaga ttcagaagca attaaaagaa gttcgtgagt
     8521 atgacgagaa gttgggtcac ttagcattaa aacgaattga attagactta gatgatggtg
     8581 taattgtcaa ttatgataag ttgcagcgtg atcctaaaac cggcgagaaa ttccaaattt
     8641 ttgcaaaagg ggtcaaagaa ccaaaaaaat agttatctaa gaaagttgtt ttctaacaac
     8701 tttctttttt attcttaaat tgcgtgcgaa attatccaaa ttatgtcaat ataaggggta
     8761 tagaaagaga acgattaggt gagattgatg acagatataa atacgcaaca aattgaagaa
     8821 aaattaaatc aacgttttcc taattatgga tgtaggaaaa tcattttttg gtttgattca
     8881 aatcaggatt ttcttgaaga aattgactca ttggtcattc aaaatgcaaa aatctatcga
     8941 ttagaaaagg acgcacaatt taaaactaag cgtttattgg aatatgaaga tcccgaaact
     9001 aactatttaa tctatgcgcc atttcctcgt tgggatgata aagatgaaga taatcatttg
     9061 ttgagtgtat tgaattattc agaggaattt agtgcagacc gcttagcgat tatcatgaat
     9121 gaattggcta ttccacttag atttcatcag acagttaaac agtatcgtaa tttctttaat
     9181 gcaaaggatc gcatgagaag ttttggtgaa tttagtacgc atatgacaat taataaatca
     9241 gaaacgattg aattaatgat aatggctgtt ttgttgaaat cgaaaagtat tcgattaaat
     9301 gatttactgc gtgtagttct aaaaagcttt acagaaagta gggagaatcc tttaagggaa
     9361 cttgataagt atcagttaac agctaaattt tgggaattta ttcaaatgaa atttggcttt
     9421 acggatagcc agccaacatt ggaaaagctg acgatgtgtc tttatttgaa ctatttttat
     9481 tatcaaattg atcaagagat gccgaagaag ttcaaaggat ttgaaatact acatttgaaa
     9541 gcaaacatta ttactttcat ggatcagttt atgaatgata cacgttacat ggaaagcttt
     9601 gaacagctat ctaaaaaagt atatcaggct attgatggtg atggattatt agacagtata
     9661 gaaattgatg atctaattga agcagacgta ttttctgaga ttcatgcaaa aatattaatg
     9721 ttttatgcag aacgattaaa ggcttctgac actacaacga aaattgcagg acaaacaatt
     9781 tttgatagtt taaagcagcg cgaaaaaatg catttcgcat cggattatat ttatgagtat
     9841 caattattga aacatgctta ttatcttgtg gataaaaaca gattgtgctt gccgatgaac
     9901 atggataatt tgctaactca atatgagaag gaaatgtatt gggttgatac gcattatcgg
     9961 aaatttattt ggaatagtga tcatatagaa gaaaagcaaa aatatggcca attgtatcga
    10021 ttagttgaag caaattattc gaagtttctt gatcaatcag gacaaatttg gaatcaaact
    10081 tttgaattta agaatcgtcc atccattcga aaattttata atcaagctgt aatgaaacgc
    10141 tctgttaaaa ctgtagttat tatttcagat gcttttcgat atgagttagg gatgaattta
    10201 gaacgtaagt tagttagaga aaagaagtat tcaacaaaca tggctagctt tctttctgta
    10261 ttaccttcag ttactgaatt cggtaaagcc gccttattac caaacaatca actaacttac
    10321 aatgaaggaa cagaagtact gattgatggg aaaaaaacga atggtttatc caatcgaaat
    10381 gcgattctcc agcaacgaga tgcaaatgct gtagctttaa attatgatga tgtggttaag
    10441 atgaataagg ctgaggtacg agaagtattt aatggcaaat cagtcattta tatttatcac
    10501 gatcaagtgg atagaacagg tgatcactcg aatgaaaatc gagtttttga ggcctgtgat
    10561 ttagcgataa gcgaaatttc ccgacttatt aatcgcttgc ataatgatgg cagtatctat
    10621 cgttttatag ttacggctga tcatggattt atttataaaa gaagccacgt tgaagaagta
    10681 gataagattg aaaatccatc tagaaatgaa aaagatcgtg tagaacgtcg ttttattctt
    10741 agtgaagaag aatacgaaat aattggtacg aaaaattact ctttgggaga ttctttagga
    10801 aatgatgaca ctagaaacat ttgtgtcccg attacttcaa gtatttttaa gaaagctggc
    10861 gggggacaaa gctacgttca tggagggtca tctattcaag aaatgctagt ccctgttcta
    10921 gaaattacgg tagccaaagg tagcaatgca aaggaaccag tagcaattga attaatgact
    10981 agcaaccgca gaattacagg attatcaacc acgttagaat tctatcaaaa aaatgcaatt
    11041 agtgatgttt atgaccccgc atctttctgg ctttattttg aagatgaata tggtgaacgt
    11101 atctcaaatg aagaaatcta tgttgcggat tcaaaaacag aagatgcaaa tcagcggttt
    11161 actaaatttg tttttgaatt tgtaaatcgt cgatatacag ttgaagatac ttattattta
    11221 gtgataaaaa atcaaaaaaa tgatttgacc gagcgaataa aattcattat agataatcct
    11281 tttgcagaat catttgattt tgatatataa aaacgaggta tccacaattt tttgtggata
    11341 ctttttcttt tgtattatca agcctactgg ttaagtatga tatactttaa ttgctattat
    11401 aatgactgaa cacatggagg aattctagtg gaacaacagg aaattttaaa gttatctgaa
    11461 ttctttcccg gaaagattgt cagaaaagat ttaacaaaga aaattaaaga aggtgcgaat
    11521 gtacctgttt atgtattgga atatttactt ggtatgtatg cttcgtcatt ggatgaaaca
    11581 ttggttgaaa aaggtgtgga aaatgttaaa aatattcttg ctcgaaacta tgtgcgtcct
    11641 gatgaagcag aaaaagtaaa agcaaaaatt cgtgaattgg gtggttatac agttattgat
    11701 aaagtaactg tacatttaaa tgaaaagaaa gattgttatg aagcagaatt ctctaacctg
    11761 ggattaaaaa atgttcaaat tagtgaagag tatccgaaaa aatttgatcg attattaggt
    11821 ggggggatct ggtgtattgt ttcgatggaa tattggtatg atgagaatga tcgttggaat
    11881 agccctttta ttgcaaaaaa ggttgatcca atccaaatgc ctggtttaga cattgaggct
    11941 tacaagatgg ctcgcaagga gtttactaca aatgaatgga ttgacttact tctacggtcc
    12001 attggtatgg aacctacaca atttaataaa agggtaaaat ggcttcattt agcaagactt
    12061 attccattaa ttgaaaataa ttttaatctc tgtgaactag gaccacgtgg tacaggtaaa
    12121 tcgcatgtat ataaagaaat ttcaccaaac tcaatccttg tttcaggtgg acaaacaaca
    12181 gtagcaaacc ttttctataa tatggctagt cgtacaatgg gacttgtagg tatgtgggac
    12241 tgtgttgctt ttgacgaagt tgctgggatt aaatttaagg ataatgatgg cgttcaaatc
    12301 atgaaagatt acatggcttc aggatctttt gctagaggca aagaagaaaa gaatgcttca
    12361 gcatcaatgg cttttgtagg aaatattaat caaagcgttg atgtaatatt gaaaacttcg
    12421 aatttatttg agccatttcc ggaggttatg gggacggatt cagccttttt ggatcggatg
    12481 cattgttatt taccaggttg ggaaattcct aaatatcgac cagatttttt tacaaatgac
    12541 tatggcttca ttacagatta tttttctgaa gtcatgcgtg agcttcgaaa agttagtttt
    12601 ggcgatagtt ttgataaata tttcaaactt ggttctcaat taaatcagcg ggatacgatt
    12661 gcagttcgtc ggattgtatc tgggatgatg aaactgttgt atcctgatgg tagatttgaa
    12721 aaaagtgatg tcgaagaaat actaagattt gctcttgaaa tgcgtcgtcg tgtaaaagaa
    12781 caattaaaga aaattggtgg aatggaattt tatgatgtca atttttcata cattgattta
    12841 gaagaagagc gagaagagta tgtttctgtt cctgaacaag gtggaggaac tttgattcca
    12901 gaagggttag ggaagccagg gcatttgtac acggtatctt atggcaaaac tgggcaatta
    12961 ggtacctata aattggaaac gcaaatgact tcgggaaatg gaaagtttga acgtactggg
    13021 atcaattcag atgctggagc aagagaagcg atggattcgg cataccgata tttaagagct
    13081 aattatggaa gtgttagtca gtctattagt ttgacgacaa aagattttgt cgttcatgtt
    13141 caagatatga atggcattgg tatgacaaaa acattatcgt tatcaactat aatagcttta
    13201 tgctcagttt ccttaaataa acctgtatta ccaagtttag ttattttagg ggatattaca
    13261 attggtggtt ctatgattaa ggtagataat ttagctaact gtttacaagt agcaaaagat
    13321 gccggagcga aaaaagtact tgtacctatg ataagcgctg ctgatattgg aactgtaccc
    13381 ccagaattaa tgggtacctt taatttgatt ttttatcaaa cgccggaaga tgctgtcttt
    13441 aaagcccttg gggtggaata gacaactttt ccatttgctg gtttgactac gtaccaatct
    13501 atattactta ctagattagt cgttaatcta ttcggatcga atgggcaaaa tatttttgtg
    13561 tatgtccgtc cttgatcatg attccaaaaa gaatcattgg gggctgtggc gtaaatatta
    13621 tact
//
LOCUS       DAZKOB010000072        13272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000072 DAZKOB010000000
VERSION     DAZKOB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.072"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2236)
                     /gene="glyS"
                     /locus_tag="ACG8M6_002015"
     CDS             complement(161..2236)
                     /gene="glyS"
                     /locus_tag="ACG8M6_002015"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0457668.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2238..3155)
                     /gene="glyQ"
                     /locus_tag="ACG8M6_002016"
     CDS             complement(2238..3155)
                     /gene="glyQ"
                     /locus_tag="ACG8M6_002016"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0457669.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3541..4359)
                     /gene="recO"
                     /locus_tag="ACG8M6_002017"
     CDS             complement(3541..4359)
                     /gene="recO"
                     /locus_tag="ACG8M6_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0457670.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4502..5401)
                     /gene="era"
                     /locus_tag="ACG8M6_002018"
     CDS             complement(4502..5401)
                     /gene="era"
                     /locus_tag="ACG8M6_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0457671.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5416..5811)
                     /locus_tag="ACG8M6_002019"
     CDS             complement(5416..5811)
                     /locus_tag="ACG8M6_002019"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0457672.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5795..6271)
                     /gene="ybeY"
                     /locus_tag="ACG8M6_002020"
     CDS             complement(5795..6271)
                     /gene="ybeY"
                     /locus_tag="ACG8M6_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0457673.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6300..8492)
                     /locus_tag="ACG8M6_002021"
     CDS             complement(6300..8492)
                     /locus_tag="ACG8M6_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0457674.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8516..9487)
                     /locus_tag="ACG8M6_002022"
     CDS             complement(8516..9487)
                     /locus_tag="ACG8M6_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0457675.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10192..11571
                     /gene="brnQ"
                     /locus_tag="ACG8M6_002023"
     CDS             10192..11571
                     /gene="brnQ"
                     /locus_tag="ACG8M6_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0457676.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11853..13193
                     /locus_tag="ACG8M6_002024"
     CDS             11853..13193
                     /locus_tag="ACG8M6_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0457677.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         3668 a         2839 c         2441 g         4324 t
ORIGIN      
        1 tttcattttt atttcctcac ttttcatgag cgttttgaaa attcgaacaa agaataaaat
       61 aacaaaaatt attaaaatag aattaataac agctatttat aacaaaaaaa acagagacac
      121 agcacgtttc gtgcatgctc tctgtttttc aaacaaaaga ctattttgtg atgatttgat
      181 ccaagcttgc taatgacaaa gccatggcag ctatttgact tagttgtgtt agtcggttct
      241 tacgtactgc tggttcatca gccatgacca tcgtttgatc aaaataattt tcgatcagtg
      301 gacgaagagc gacaagctgg tcgtagtttt cgtggatcgt ttggtcagca aaacttgctt
      361 tcattgcttc tactgctttg tgtaattctt tctctgcgtc gttttcgaac agctcagggt
      421 ctacttctga ttgtttttca agctgcgctt ttttcgctaa gttcacgaca cgtgtcaaag
      481 cttccattga aggtttgaag tcttcatctg caagatgttg tttgaacaat tgtgctgctg
      541 caaatacttt tgtcagatct ttttgttccg cattcaaaac agcatcgatg acatcatgac
      601 gaatattctt cgttgtcaat aattggcgca gacggccttt gataaagtcg atcacttgtt
      661 gttgcccttc tgataaacca atgccgaaac gatccgtatc tttactgata acatcgctaa
      721 tcgtttcttg cagaacagac aaaggaaagg cccatttttt cgattcgatg atccgaacga
      781 caccgtatgc ttggcgacgt aaggcataag gatcgtttga cccgcttgga atcatcccaa
      841 ctgcaaagaa agaaaagatg ctatctaatt tatctgcaat cgctaaaaca gaacctactg
      901 tagactgagg tagctctcct tcactggata taggcatgta atgctcacga atcgctgtcg
      961 ctacagcagg tttctcgcct ttcagaagag catatttttc gcccatgatc ccttgtagtt
     1021 caggaaattc ccctaccatg ttggttacta gatcaaattt atagatttca gcagctcgtt
     1081 gcaaatccat caattcttcc tcagatagtc ctacttgttc gccaatgata cgggcaatga
     1141 cttgtacccg ttgcattttc tcatagatac taccgatttt ttcatggaaa gtaacagttt
     1201 ttagtttttc aacgtattgg tcgattgaag ctttcttgtc ttcattataa aagaattccg
     1261 catcttctaa acgagccgtc aatacttttt gattgcctcg aaccacatct tccaatttta
     1321 cccgatcacc attacgagca gaaataaaat gaggaagcag cataccttga tcattccgta
     1381 cctcgaaata acgttgatgt tctttcatag aagtgactag tacttcttca ggaacagaaa
     1441 gatatttttc ttcgaagtcg ccagaaaaga cagttggata ttcgactaaa ttgttgactt
     1501 cttctagtaa atcttcgtct agatcaataa cccagtgatt tttttctgct aattcgtttg
     1561 cttgtgtaac gatcatttct tgtctttttt gcggagacgc aatgacgaat tgttcattca
     1621 atttttcttc atattctttt gcatgcgcaa atgtcacatc ttcgcctaag aaacgatgac
     1681 ctcgagaggc tctgcctgtt ttgacatcca agatttcgaa tgggattact tcgtcatcta
     1741 acaatgccac gatccagtga atagggcgga tatattcaaa atcgtattct gcccaatgca
     1801 tcgttacagg gaaagtcaaa cttttgatca cccctgctaa gcctgtcaat acttcttttg
     1861 cttccttacc atgagtgtat ttcgttacat agacatattc cacacctttt aattctctaa
     1921 aagtgatagc gtctgtcgtc agaccttgtc cgcggacaaa tccttccgct gctttcgtcc
     1981 agtttccatc tgcgtccaaa gcaatttttt tggctggtcc tttgacttct tcttcactgt
     2041 ccgcttgtcg ttctggaata tttgttactt tgattgctaa acggcgcggt gtggaaaatg
     2101 tttcgatcgt atcataagtc aattggtttt catctaaaaa ttttgctgtt ttttcttcta
     2161 attgtttcat gcttggtgtg acaacatggg caggcatttc ttccagcccg atttcaagta
     2221 ataagtcttt tgccatctta ttcagcctcc ttcacggttt ctggatttgc tgtgttttta
     2281 ttcaacaaag ggaatcctaa tttttcgcgt tctgccacaa agattttggc tacagcacga
     2341 gccatgttgc ggatgcgaga aagataaccg gcacgctctg ttacggatac tgcaccgcgg
     2401 gcgtctaata agttgaatgt atggctacat ttcaagatat aatcatacgc cggatgaaca
     2461 agattttcat cgatacagcg tttagcttct ttttcaaaac gatcaaagtt gcttagtagc
     2521 aagtcttggt cgcttacttc aaaagaatat ttggaatgtt catattctgg ttgtttgaag
     2581 atctcgccat atttcacgcc atcagcccat tctaaatcat agacactttc aacttcttgg
     2641 atataagaag caagacgttc taatccataa gtgatctcag aagttactgg tttacaaggc
     2701 agtccaccaa cttgctggaa gtacgtgaat tgtgtgattt ccattccgtc aagccatact
     2761 tcccaaccca caccggcaca gcccatggat gggttctccc agttgtcttc aacaaaacgg
     2821 atgtcgtgtt ctaatggatc gattcctaaa aggcgcaagc tttctaaata aagctcttga
     2881 atattctcag gtgaaggttt catcaccact tggaattgat gatgctgata caaacggttt
     2941 gggttttcac cataacgtcc atcagctggt cggcgagatg gctctacgta agcagcattc
     3001 catggttctg gtccgattgc ccgtaaaaag gtatatgggc tcatcgtacc tgctcctttt
     3061 tctgtgtcat aagcttgcat cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt
     3121 aaaatcatat cttggacggt caatttttta ctcatctgtt ttgccccttt caattttaaa
     3181 gcgttgctgt caaataagaa tacaggaaag aaaacgacaa aaaatcccta tgtctgatta
     3241 cagacatagg gacgaataat tcgcggttcc accctacttc cgaataaaga ttcggcagct
     3301 tcatttatcg tactcgcaag tgcctcttca aaaatcgttt gggtccggct cccactctcc
     3361 cgaactcgct aaaccacgtg ttttttactg atcttggtca atgtacgtat tcattttgca
     3421 gttttcttgt ttctataata gatcctatca ctgaacgatc tatttaatct atggttaata
     3481 atagacggaa ctccctgtgt ttgtcaaatg gttttgattt tttgttgtat gttttattta
     3541 ctattcttct ttttcaggtg tctctttttc tttttcagac atgtctttct ctgacgtctt
     3601 taatacgtgt gcccaagact tcatttgatt gatgaacttt ttgcttttca aatggatacc
     3661 gacatattca tcatacagct ggtcgatgag ttcacggatt gcttgtttgg tttcttcttt
     3721 cagatggatt ccactaattt tatcataaga aatagctgaa aacatccgaa cgaaataaac
     3781 agctcgagga tctgcatgat aacggtgatc gtccatcgac caatgtcttt cgcatatcac
     3841 accaccatat ttggaagaat agtcaaattt tccttttgtt tccccgcata cgacgcagtg
     3901 tgtccagatt ggtgcaattc cgaagcgttg cagtatttgg acttcaaaaa tattcgtgat
     3961 gatttctgca tcgtctcctt ggtccatccg ttgcagtgct tgttgcgtaa actggaacaa
     4021 atgcgggtcg tactgatgat cttcgattgc cgcatctact agattcaata aatatgtgcc
     4081 atagccgctc aaaaagatgt cttcttggat gtgtcgaaac gcatggacat ctttgacact
     4141 gcttaaaaat gacaaacctt cacttcgaaa attcccgata taaactgctt cagtaaaagg
     4201 gagcaaagcc ggagctaagg gctgattttg ccgatggatc tctttgatat aaaacatcat
     4261 tttgccagaa gattctgtaa agattttgac taatttatct ttttctttgt aattacggct
     4321 agataaaatc aagccttttg attctgcaag ctggctcata ttctccctcc taaaaaataa
     4381 atctggaaaa gcagattctg ccgatccaga tcatttatgt tgcgatcttc cgcagctttt
     4441 ctcaacacgc ggatttttat tcagtttatt tctataccta atttacaaga agctcagaat
     4501 atcagtattc gtcttttcgg tagccgaagt cttgcagata gacttttttg tctcgccagt
     4561 ctttttgaac tttgacccaa agttctagaa agactttgtc tcctaaaaga ttttcgatat
     4621 cttttcttgc tttcgttcct acttgcttca gcatttttcc gcctttgccg ataatgatcc
     4681 ctttttggct atctctttcg acaataatcg tagcctggat atggatttta tcattctcgt
     4741 ttcgcttcat cgaatcaaca acgactgcga cagaatgagg cacttcgtct cgtgttaaaa
     4801 acaagacttt ctcacggatc aattcagaaa cgatgaagta ttcaggatga tcagtcactt
     4861 gatcatctgg aaaatattgt gggccctctg gcatttcact gactaataca tccatcaaac
     4921 gttcgacgtt gtttccttct gtagcagaga ttggaatcac ttgtgcaaaa ggcatctgcg
     4981 ttgaataatc ttcgatgatt cccaacaact cgtctggatg gattttgtcg attttattga
     5041 taatcagata aacaggagaa tgcacgtttt ttagacgttc aataataaaa tcgtctcccc
     5101 gaccgcgttt ttgatcagca ctaatcataa ataagaccgc gtccacttct ttcaacgcac
     5161 tgtaagctgc ttctaccata aaatctccta aacgatgctt tggtttatgg attcctggtg
     5221 tatcaataaa aatcaactga gcttctggtg tagtgtatac tccttggatt ttatttctag
     5281 ttgtctgtgc cttgtcactc atgatggcaa ttttttgacc cacgattcga ttcaataatg
     5341 tggatttccc tacatttggg cggccgacga ttgcgacaaa ccctgattta tgttctggca
     5401 tatatgttct cctttttaat taaacaacaa atgatatatt ttagggacaa ataaaatcaa
     5461 cccaacgatg acggtaaaac aagcggtcac taaaactgct ccagctgcca tatctttgat
     5521 ttttttccca atcggatgaa aatgaaaatc cgtaaacatg tccacgacat tttcaaaaac
     5581 agtattgatg atctctacta tccatacgag aaaaacagct aaaagcagcc ataaccattc
     5641 tatccgatca agttggaaaa tcacgcccat aaacaaagcg aggattccga acgcaacatg
     5701 tttcctcatg ttgcgttctt cctcaaaaac agttttgact ccttggacgg caaattccac
     5761 ggacatcagg aaatgtttgt ttttctctac gtttttatct tttaagtcca taggcatcta
     5821 agatctcttt ctgtaaacca aacatttctt tctcatcctc tggtgtcata tggtcatacc
     5881 cgttgatatg caagaatcca tgaaccgcta aaaagccaag ttcccgatca tacgaatggc
     5941 cgtaagcttc tgcttgctct gcagcacgtt cagtagagat catgatgtct cccagtacac
     6001 gaggcaatgg attctctcca tcttcaaaaa tcactgggat ttcgtcttcg ccctcttctt
     6061 ccaaggcaaa actgatgacg tctgtcggtg catcttttcc acggtaatca cgattgatta
     6121 cttggattgc tgcattgtcc atgaaagtaa cagacatttc tgtttcttct ggcaactcta
     6181 aatatgatgc tgcaaattgc agcaagtcgt cgatttcttt cattttctct tctgagactt
     6241 ggtttgtttc atccatgaag gtaatatcca ttatacatcg cttctttcta ttcttcttgc
     6301 tattcttctt gttcttcttt tatcttctca acttccgatt tcattttagg atatttgatc
     6361 cgagaatgga aggtactgct taaaccgctg atcaatgatt tttctacttt acggatttct
     6421 tttaacgtca atccactgtc gtccaattgt ccatcggaaa tacgctcttc aataagatta
     6481 tggacaaaac tttcgatttt ttcgctcgtt ggatggtcca ttgcccgtac tgccgcttca
     6541 caactgtcag ctatattgac gattcctgct tcacggcttt gaggttttgg tccaggataa
     6601 cggaattgct cttccgtaac ttccgggttg cgttcctttg ccttaacgta gaaataacgc
     6661 atcagcgtgg ttccatgatg ctgtctacaa atatcgatca ccatttgcgg catattataa
     6721 tcttccaata ttttggcacc atctgtcaca tgtccaaaga taatatgttt actgtcttct
     6781 ggcaataaga aattgtgcgg attttccgct cctgaaggca agttctcaac gaaaaagctg
     6841 gcgtgtttga tcttgccgat atcatgataa tagcaagcta ctcgagttag taaagaacgt
     6901 ccaccgattt ctgctacagc attagcactt agattggcta ccatcatgct atggtgatac
     6961 gtacctggtg cttcttccag caactgtttc aataaaggat gattgggatt gctgagttca
     7021 tttaatacga tcacactatc atcattgacc atcaattcaa tataggggtg cagacctatc
     7081 gtcagcaaga atgaaaacag tgcacctgtg aatccgcaga ctagcgttaa ccaagtcgtt
     7141 gcatccccaa agctcattcc ttggtagata atcaaaatga catccatcat cacaggaaaa
     7201 ataatgaccc acatagcagc ggataaccct tgttctgaga tccgtttgcg tctgacgatc
     7261 gttgccatga gaccagaaaa tacataggac atcaaaataa ctgtcagaaa gttcgtgcca
     7321 atcgaatcat aaaaaatgaa aagtgcggat actgcttgaa aaataccagc catgatccct
     7381 gctcttcgat tcaagaaaaa gcttaaaaca agtggagcaa aagccgcggg gtaaaacagc
     7441 ggaatataag cagcttgttc tgtttggaag agttggaaaa atttcattag tacgacgctt
     7501 actgacattg ccgtcacgta gaaaagtacg tattttgttc tttctcctgc attttggaat
     7561 tgaagtgaat tataaatcag tacaccgatt tgaagcaaaa cagctaaaac catggcaatg
     7621 agtgggaaaa tagaagtcgt ttgattcgtc aatcctagta attccaattt tttcatcgct
     7681 gaagcatcaa tttgatttcc ttcgcgaaca ataatttctc cttggaagat catcactggt
     7741 tgtacagctt ctcgtgcaga ttgttttaat tcttctgtct ttttctcatt caatacatca
     7801 ttaactacga tgccttgatc cactaagtag cgaatcattt gttgctgctc tgttgtgaca
     7861 ttcaaatatt gaatctgatc ttctgcttct tgtttaaaag cttccaattc gctttcacga
     7921 acttgtttac tcatctgttc gtcaatgagc ttcaaacttt catccctaac tgtgtgaatt
     7981 tccgtttcag tcatttcaaa tatcgtcgaa taaaaattgt tcggcagttt ttggtaaaac
     8041 gcaacgtttt ctgcattcac gttttcaaaa tttttcttca gtgtagcaat ccgctcttct
     8101 gtagtaggtt tcgggatatt ttcatcttcc ttcgccttat ctaccttgcc attataagat
     8161 ttgttgatgg tatcattcgt cttatcgatc aattcgaata attgcttgat ccgattgttc
     8221 tggtcatctg ccagatcttt ttgataagta tattctggag tcactgcttc tgctgccagt
     8281 tttcttcttt gttcggtttc ttccgtgttc tcaatcgttt tattcgcacg gatactctct
     8341 tctgctactt ggccttctct gaaatcaatg gccttttgtt tgacactact aaatgtaacg
     8401 ccgcagacaa tgataaaaaa caagaacaga aggaaaggca atgctgcctt tccgagtttt
     8461 tcttgtagtt tctgcagtgt tttattcaac atgtgcttat cctcctaagc aaagcttatt
     8521 cttgatgaag atccgccttt tcataagctt tgatgatttc ggcaactaca gggtgccgaa
     8581 caacgtcact tgcttcaaaa ttaacaaaat cgattttttt gatttgcttt aatgttcgtt
     8641 ctgcatgaac aagaccactt gtcgtcccac gaggcaagtc tatctgactt gtatccccat
     8701 tgacaatcat ttttgattga taaccgagac gggtcaagaa cattttcatc tgagcgactg
     8761 tcgtgttttg tgcttcgtcc aagataacga atgcatcatc caatgtccgc ccccgcatgt
     8821 aagctaaagg tgcgatttcg atcacgcctc gttccatcaa tcggttcgta tgatccaatc
     8881 caaaaatttg gtataaagca tcataaacag gccgcagata aggatccact ttttctttca
     8941 gatccccggg tagaaagcct aggttttccc ctgcctccac agcgggccgc gtcaaaatga
     9001 ttttttgcac ttcgcctttt tttagggcag caattgctaa tactacggca agaaacgttt
     9061 tccctgttcc ggctggccct acaccaaata cgatgtcatg tttggctacc gattcaacgt
     9121 atttcttttg accgctgttt tttacccgta taggttttcc attacggtct ttgacgatct
     9181 cttcctcata catctcaaca aaataatcta atgtaccttt ttgccccatt ttcaatgccg
     9241 tcacaacatc tggcgtactt acgtggattc ctcgtttgat caattcctgt aatgtccgaa
     9301 tgactgaggc agccaaggaa atctgggatt gctcaccaat gatctgaatg atttctccac
     9361 gtgtatggat ggttgtttcc gttgtttcct cgatcagttt tatatgcttg tcgtgtgcac
     9421 ctaaaagcat gctgacatca tcagcagctg tcaatctaat ctctaaagaa ctagactctt
     9481 ccgtcaaaaa tagacatccc ctttttttcg attcattctt tcctatttta ccatactcag
     9541 aagaaactat atagcaaata agaaaaggtt gtaaaagagt cgcttaacca tacgtaccaa
     9601 gaaacgttaa aaaatagccg tttccattcg ctaaaggtct cgttttgata aaaagaagct
     9661 gagaatgtaa gttatatgaa agtggagaac aaaatatttc gtttcattca acaagtattc
     9721 tctaggtatt tttcctgttt caaaaaaaga aaacaaaata gttaaaatat tcttgcattt
     9781 caaaaaaaac aggtgtatta ttacgtcaac aaataaacat acagcaaagc gtcgaggaaa
     9841 acagtaggct attcaacgat ttattcccag tgagtcatgt aagtgagaaa tgacaataaa
     9901 tcgaatagac gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt gtggctgcct
     9961 ccattatcag gctagcggat aactttatca aaaagcgtcc gtgaatgaag aagctttttt
    10021 aagcgaaaaa aggtggtacc gttctagaat ctagagccct tttcccaagt ggaaaagggc
    10081 ttttttttat tttaaggtgg tggttttcat ggaatctgat attagtggca ttactaattg
    10141 ctgcaacgtt tatatcgtca atcaaaatga taattattgg aggaaaaatt tatggaagaa
    10201 acaaaaactt attcatggaa acaactattg atggtcagtt cacttatctt cgggatgttc
    10261 ttcgggtcag gtaatctaat ctttcctgtc cacttaggtc aaatggctgg cgcaaactgg
    10321 gtcagtgctg gcgtaggatt tgctatctca ggtgctcttt tcccattact agctatctta
    10381 gctgtagtag tgacaaaaag tgatggtctg tatgacttag ctaaacctgt cggaaaatgg
    10441 tatgctgcct tgttcttaat ccttgttcat ttgacgatcg gaccattttt cggtacacca
    10501 cggacagctg ctactgcttt tgaaatggct gctaaaccat ttttgcctga aaaatataat
    10561 tcattaggta tgtttttatt ctctgctctt ttctttttac ttgcttattt ccttactgtc
    10621 catccaacac ggttggtcaa atacgtcgga aagtatctaa actccttgtt tcttgcatta
    10681 ttagcaatca tttttgtcgt ttctttattc catccaatgg gaaatctaaa tcaatctgct
    10741 gcagatactt atcaaacaaa cgctttaaca aatggtgtcc tagaaggtta taacacggtt
    10801 gatgcagtag ctttgcttgc cttaagtgtc acttttgtcc atgcagtccg tgaccttggt
    10861 ttcaaagata aaaaagtcac agaattaacg gcaaaagctg gtagcctgag cgttgcgcta
    10921 gaagtcatga tctattttgg acttgttctt ctcggtgcga tgagtttgaa taagatgtct
    10981 ttatcagaaa atggcggaac tgcactttct cagatcacta atttttattt aggtaacttc
    11041 ggcgctagct tcttaggagt gatggtcaca ttaggtgtct tcactaccgc tatgggactc
    11101 gttgtttctt ttgcgcaaga tttccataaa ctgttcccta aagtcagtta tatgacatgg
    11161 ctgcggttaa cgactttcgt ctcttttgtc gttgcaaatg ctggtttaga caatatcatc
    11221 caatggtctt taccagtatt gatgcttttg tatccattgt cgcttgcgtt gatcttgctt
    11281 tccttaacag caaaattttt ccaaaagaca ccatttgtct atcaagtaac gatgttgttt
    11341 gctgctgttc ctgccgtatt ggatatgctg gcaaattcgc ctgctttagt gagtcaacaa
    11401 agagttgtag caagcatgct cgaattttat catcatcacg ttccttttgc tgcattagga
    11461 cttggctgga tggtcccaac gttacttggt tacgctggca gcctactgtt ttattacgcc
    11521 tatcgcctat cagggtataa acaagaagca aatgaactac cagaagaata accaaatcaa
    11581 acacaattta acataagaaa tattaaacag gaaaaaacca gctagatgaa agaatcctat
    11641 tctttcgtct agctattttt tagttctctt ttttacgcgc acaaaaaaac tgcggacaat
    11701 gattgtccgc agtttaaatg ggctctataa tatctagtgt tggtgacgcc ttctgttgcc
    11761 aatacttttt cttttttcta ataaacgcaa acagacacct tgaaatcctt tagaataaag
    11821 tcgacgaaaa ccattcaaag gagtgtttca agatgtccta ttcagagttt accaaagagt
    11881 tactagatat tctagaccta aatcttatct ttcatgaaga tgcctttcgc aaagagcgaa
    11941 tcaatgacga aacatgtttt gtttttgatg gcacattgac ctatcaacca gaggaatgct
    12001 tccattgtca ctatcaaaat aagcaaacga tcattaaatg gggctggaaa aaagtatcca
    12061 tcttattgaa tgatgtcagt aactacaaga ccattctgcg gattaacaaa cagcgcttca
    12121 aatgtaaaca ctgtggaaag acttttttag cagaagattc agttagtgat cgtcgctgct
    12181 ccattgctcg cagagtcaaa caagccattc ttgagctatt aagcgaacct ctttctatgt
    12241 ctttgatcgc acgtatgaag cacatttctc cgaccactgt cattcgaata cttcgcagcc
    12301 ttcggcctaa aaccgtttct ttgaaccagc cgttaccaga agtggtgtgt ttcgatgaat
    12361 tcaaatcggt gaaaaacgtc tcgggagcca tgagctttgt catgatggac ggcaagaccc
    12421 atcaactgat tgatattgtg gaaaatcggc aactaaatcc tttacgcgac tatttcttgc
    12481 gctaccctag aaaagtccgt gaacaagtac gtctagtggt ttcagatttc tatacaccct
    12541 atcgcacatt ggtgaaagag tgctttccga atgcccgaat cgtcgccgat cgctttcata
    12601 ttagccaaca catcggtcgg gcctttacca accaccgaat tcaagtaatg aagaccttta
    12661 aaaaaaggga tcgtcggtcg aagcacttaa agaaatactg gagactactt caaaagaatg
    12721 cgtgggaatt gaaaggtcag catcgctact ggcgtccgtc atttcgagat catctgacag
    12781 aagcagagat agtcgatcgg ctattatctt atgatgactc cttaaagcga ggctacgaag
    12841 tctaccagga ttttctttcc gctatcagaa gacaagatgt tcctgaattc gtcgcgctcc
    12901 taaaagaaga ctacaaggag ttgccagagc actaccaacc agtgttcacg accttcaaga
    12961 aatatcgaac agaaatcaaa cgagcgttgc gagttcccta ttccaacggc cctatagaat
    13021 gtttgaacaa ccatatcaaa gtactaaaac gaattgccta tggctttaga aactttcaaa
    13081 actaccaaga acgaatcttt ttatatcgag gaaaatattt taagaaaact aagaacacta
    13141 cccaactgac taaagctaga acaacaacac ggctagacaa gatagctgtc tagccgtgtg
    13201 agtatctcga ttattctatt gaatttcggc tctatacttt ttgatgttgg tgaacttcac
    13261 caacatcttt tt
//
LOCUS       DAZKOB010000073        13209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000073 DAZKOB010000000
VERSION     DAZKOB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.073"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..1013
                     /locus_tag="ACG8M6_002025"
     CDS             144..1013
                     /locus_tag="ACG8M6_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0457678.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1222..2247
                     /locus_tag="ACG8M6_002026"
     CDS             1222..2247
                     /locus_tag="ACG8M6_002026"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0457679.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2286..3236
                     /gene="manA"
                     /locus_tag="ACG8M6_002027"
     CDS             2286..3236
                     /gene="manA"
                     /locus_tag="ACG8M6_002027"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0457680.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3330..5033
                     /locus_tag="ACG8M6_002028"
     CDS             3330..5033
                     /locus_tag="ACG8M6_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0457681.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5035..7068
                     /locus_tag="ACG8M6_002029"
     CDS             5035..7068
                     /locus_tag="ACG8M6_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0457682.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7079..7519
                     /locus_tag="ACG8M6_002030"
     CDS             7079..7519
                     /locus_tag="ACG8M6_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0457683.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7719..8846)
                     /locus_tag="ACG8M6_002031"
     CDS             complement(7719..8846)
                     /locus_tag="ACG8M6_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0457684.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8849..9526)
                     /locus_tag="ACG8M6_002032"
     CDS             complement(8849..9526)
                     /locus_tag="ACG8M6_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0457685.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9790..11556
                     /locus_tag="ACG8M6_002033"
     CDS             9790..11556
                     /locus_tag="ACG8M6_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0457686.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11571..12008
                     /locus_tag="ACG8M6_002034"
     CDS             11571..12008
                     /locus_tag="ACG8M6_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0457687.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12021..13172
                     /locus_tag="ACG8M6_002035"
     CDS             12021..13172
                     /locus_tag="ACG8M6_002035"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0457688.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4489 a         2293 c         2731 g         3696 t
ORIGIN      
        1 ataagagttt ttactcatta atttacaaaa aacaagcaaa atgcgaattt tctcacattt
       61 tttttaacaa aatcaaagtt attgcttgaa agaagaagaa tccatcgtta aaataaagct
      121 tgacgtagag gggagcaaga aatatgaaag attttcaatt gggggctgcc acctatttaa
      181 aaagtgtagc cattcgggtg aaagaccgag ataaaatgat tgatttttac aagaacgttg
      241 ttggctttga tttgaaaaga gaagaaaacg aattagccat tttaggaaca gaagaagctg
      301 gaagtgaaat gttgctgctg gaagaaagtc ctagagcgaa tgatcacatg ggggtgatca
      361 aaaagctgaa ccgtttttca ttgattatcc ctagcacaga agaaatggca gatatccttt
      421 gtcgtatccg taaaatgaat tatccaatca aagaagcttt agaagatcat ggacgtatgg
      481 ggattttgct cgtagaccca gaagagaatg aattggaaat ttaccatgaa gagaataaga
      541 acgaccaaag agaaattcca gaaacaatgg atcaagaagc attattaaga aaatctgaag
      601 aaaaattcag taaactatca acaggttctt attttgaaaa aatccattta aatgttacta
      661 acttaacaaa agaacgccaa tttcttaatc atgtacttgg tttgaaggta cataatgaag
      721 aagagggcat ccatgtattg aatgaaggag atttccatgt tggtttgaca gaagctagcg
      781 gaggcgcaat tgatttgcct acagatgaag tgattggact tgattttctg aaattcatga
      841 tcgatactga aagcatggaa ggactgattg ctcacttgca gcaactatca gttgatttct
      901 tcttagacaa aaagcacaca gtactcacaa tctatgatgc tataggaatc gaatggtggt
      961 tcgtcagtga gccaaaagaa aagaataaaa ctgacattcg atgcgacgct taactttttc
     1021 tttcttaacg attgtcgcat gtctcgtcat tttatgaacg gtcatactat cgcttattct
     1081 agagatagaa agagaaaata gtataccggg ggaaactgcg cggtttttcg cgcagttttt
     1141 ttgtgtaaaa aaggttgatt cctcatagat taagctttaa gcttggactg taagaatctg
     1201 atagaaaagc aggagaaaaa gatgtatcca cacacgaaac agcttatttt gaaaaagatg
     1261 gaagaaggat tttatccagg tgtagcagtc gccttcataa aagaaaatca agtagaaaca
     1321 tttacagccg gctttgccca agtcgttcct gaaaagcagc tgatgacaga agatctgttg
     1381 tttgatgcag cctcactgac gaaagtcgtt gcaaccactt cattagtttt acgtctatta
     1441 gaagatggaa agatagaact cgatcaaccg cttcataaat atcttcctga atttgaaaat
     1501 cagacaatca ctttgcgtca tttactgaca catacttctg atatcaaaac atggatacca
     1561 aacagagata agctatctca ggaagaattg atcgccgctt atttgaagct agaaccaggt
     1621 gagcaactag gaaaagaagt aaaatataca gatgccggta cgattcttct tgggttgatg
     1681 ctagaggaaa tttaccaaga aactgcagtg gaaatctttc aagaacaagt attggaacca
     1741 ttaggaatga tgaacagctt atttcttcct tttccttcca aaaaaatcgt tcccacagaa
     1801 caactgcctt cgggtgaggt tttgcgtgga caaactcatg atccgaaagc aagagttttg
     1861 gctgaacatg caggtaacgc gggtctcttt attgatttga aagacagcat tacttttgtc
     1921 caaatgtatt tgaacaaggg aaaaatccgt aatggtcgtt ttttagaaga agagacgatt
     1981 gattctttac tgcaagatca aacaccgaaa aaaaatggct atcgttcttt aggatgggac
     2041 ttaaaaaagg atgtagaaga cggtcatgcg attctttttc atactggtta tacaggtacg
     2101 tttttcttga ttgatataaa aacacaagaa gcgttgatat ttttgtcaaa tcgcgtacat
     2161 cctgtcgatc atcgtgcgga gtatctcata tgtcgagatg aaattgttga aaactttttg
     2221 aaagagaaaa cggtattacc gaaataatgc tataatagct atgaaaaatg agaggagttt
     2281 gaattatgca ggaacctatg tttttaaaac cagtttttca agaaaaaatt tggggaggaa
     2341 gtcgtttacg ttcagtcttt ggttttgata tccctaacga taaaatcgga gaagattggg
     2401 cgatcagtgc gcatcctcat ggtgtcagtg tagtagaaaa tggcgaattc aaagggaaaa
     2461 gattagatga actatggtca gaacacaagg aattgtttgg ccatccttca gaaccagtgt
     2521 ttccattatt gatcaagatc ttagacgcag aagatgatct atctgtacaa gtacatccag
     2581 atgatgccta tgggatgaaa catgaaggtg aattaggaaa aactgaatgc tggtatatca
     2641 tcgatgctga acctggtgca gaaatcatct atggtcatca tgcaaagaca agagaagaac
     2701 tagctgagat gatcaaagat gggcgctggg atgacctttt gaagaaagta ccagttaaaa
     2761 aaggtgattt cttttatgta ccaagcggaa cgatccatgc tattggtaaa ggaatcatga
     2821 ttttagaaac acagcaaagt tctgacacga cttaccgcgt gtatgactac gacagaaaag
     2881 atgcgcaagg acaaacaaga gagctgcata tccaacaatc gatcgatgta acaactgtcc
     2941 ctgcgaaaac accagaatta caaatcaaag aagtccgaaa agggaattca tcgatcgtca
     3001 cttatttaga aactgagttt ttcaatgtat atgagtggga tattaaagga atcacatctt
     3061 tcaaaaaaca agccccttat acattgatga cggtaatcga aggagcagga gaccttgtcg
     3121 ttgacggaaa aacatatcca ttagaaaaag gaacaagctg tattattcca aatggcgttt
     3181 ctgagtgggc ggtccaaggt gaactagcaa tcattgcgac tgaaccaggc aaatagttcc
     3241 cagatagctg aaagcagcag acggtttttc gtttgctgct ttttctacat aaaatccgtc
     3301 catcaataaa tagaagtatg gtgaaaaaga tggaatacaa atggaaagca aacaacagca
     3361 gtcgcgtgtc tatgaaacaa ctgagaaaag ctggacatca cttaaatgaa atgatcatgc
     3421 caaatctaag tctgtttatc gcttggggaa tcttgtcatt agcaacaaaa ggtgtgtctg
     3481 gcgatttgca caccacatta gaagaggtat gccattggat gatccagctg cttttaccca
     3541 tcttgatcag ttattcaggc gggaaatcgg tagggggaca aaaaggggca gtcgccggtg
     3601 caattgcttc tttagggttg atcgctcgtt ccaatgctcc acaaatcgta ggagcgatga
     3661 ttatcgggcc gcttgccggc atcgtatatg aacaatttgt ttctagattc cacgtgaaat
     3721 tcaaggctgg atatgaaatg cttgtcagca acctactagt aggactgact ggtagcgtta
     3781 tttgtataat tggaatcgtt tttattgaac cagttttaaa tagtgttcac ttgttgctcg
     3841 cctccgttgt gtcttggcta gtcacaatga atctgttacc acttgtcagt ctgatcttag
     3901 aacctctaaa agtactattt ttcaataatg caatcaatca tggtgtattg acacctttag
     3961 gaatcgaatt ctcacaaaca gtcgggtact cttccttatt tttgatggag gcaaatcctg
     4021 gacctggttt agggatctta ctttctatcc tttgttttgc agaaaaacag gaaaaagtaa
     4081 atgcgagtgg agcattgatg atccatgcga ttggcgggat tcacgaaatc tattttccat
     4141 ttgtcttgct gcgaccgtat ctatttttag ctgtaatggc tggtggggca agcggcacgt
     4201 tgatatttca atggttagat acgggattag ctgcacctgt atcgcctggg tcactactcg
     4261 tgatttttgc caatgcgcct tatccacaac tagctggggt aagtataggg atagcaatca
     4321 gtacatttgt gaccttcatt tgtgcagcga tactattgaa attaccaaat aaagaaaaag
     4381 agaacaaaac ggtcaaagaa aggggagaaa tcttggaacc atctatcatt cattccgtca
     4441 tttttgcttg cgactcaggt atgggatcaa gtgctatggg agccgccctg ctgaaaaaga
     4501 aattagcagc agaaaaaatc gatctttctg tcggatacag ctctatctat gaattgaata
     4561 accaagtaaa tcagctggtt atcgtgcaac atgaactagc gggtcttgca agagaaaaag
     4621 tacctcaaag tcaaatctat gtactggatc actttttgga aacagatatc ttgctgtcac
     4681 gaataaaaga agcgatccaa tttccaaagc gggaagaaaa aaaagagata gcagatatca
     4741 cagaacaaaa aaagtcgcct gctaatattg tatttttgta taaaaacaac agacgcggct
     4801 cacaaactat gggcatgacg attttaagtc aattagcaaa aaaagcgaaa aaacagtttg
     4861 ttatctcaaa agaatcaata gaaaaaatca aaatccaaga agaaacgatt tatatcgcac
     4921 aaagagaact agctgtaact gaacagctag aagaaaagat ccctcggcta gttctagttg
     4981 aacattgggt agctacgaat gaatacgaaa aactcgtgaa gggagaaata taagatgtat
     5041 ttatcgccca gagaaaaaca attattagca gaatttctca gtagcccgtc acctgtatct
     5101 atccaaaaaa tgatgaattt attgaaggtc agtaaacgga ctatttaccg cgaattagat
     5161 aatcttgaag tctcattgaa atcggtaaat gcttctttga aaaaagcagc tagaggctca
     5221 tttgtcattg aggcatcaga agaagtatta gagcaattaa aagaaaatac agatgggaaa
     5281 agtaatgact tatccactgc taaacgacaa cacgcgattt tagccgagtt gttactgacg
     5341 acagagccgt tgagtcttgc tcatttcatg gaatcatatc gcatcagtaa taccactttt
     5401 tatgcagata tcaaacagtt ggaagaaagt attcgccaac tgcctttgga aatcattcgg
     5461 aaccagggat acgagatcga aggaccggaa aaataccgcc ggcttctgac agcgaatgta
     5521 ttagaattag aaatcaatga atatgaactt tttcatagta tttctttcga tgcatcactg
     5581 aattattttt ttcgattcgt tgatccgcaa catctatctc ttgcacgcaa agtaatcggg
     5641 gaagaattga tccaaaaaaa gaccaatcta agtgatagaa agcttgaaca tttggttttg
     5701 atgctgacat tgacgatgga gcgtgtgact aaaaatcatt tactgacaaa tgagacatat
     5761 aatagtctgg caaacaaaga attactacag acagctaaac gattgttttc acggatcgct
     5821 tctgagacaa aacaacttta tccagtaaat gagatcgtct tttttgcagg attattaaat
     5881 gacttcacga actcatttga tcagaatttc tttgaagaga cgttcgatac acagcttgct
     5941 tatttagtaa aacaactgat tcaagaagta agtgaagaga cgaatgtcca tttttatgaa
     6001 gatgaaactt tgtacaaaat gctgttgact catttatctg gtgtgttttc aagagccgtt
     6061 ttacaagaag agacactgac caatccgatt ttagaaaaaa tcatggttca atacgaggaa
     6121 gttgcgaatg caatccgtaa agcagtagag cggatttttc aagaaaaaaa attatccgaa
     6181 gaagagatcg cttatatggt ccttcatttt gcgaattcat tggaacgaag tccgaaagaa
     6241 agcaatatca gtattgcagg attttcgcca agtggattag cttctactag catgttggaa
     6301 ctacgattga aaaggtattt tcccttcatc caacaaattc atttctatcg gattgctgat
     6361 ttaaaaaaaa tcgatttaca agaagaatat gatgttgtta tttctacatc tattttacca
     6421 ggatacggtg gaaaatatat tttagtctcc ccgctactgc tagaagatga agtcaagcag
     6481 ctaaaagaag ctttttcgca tattgagaag aaaaaaaggc aaaaaattac gtataacagc
     6541 aataacgagt ggatgataga agatacttat gaggaaacaa tgcatttcat ggatcaaatc
     6601 aatcaattac tcggtcgttt ttttatccaa gaactggata atcctgagac gatttccgaa
     6661 acgattcgca gcttatttcc ttattttcct gaaggactag taacggatga agaggtggtt
     6721 catcgccggc tgcttaagcg atatgaacaa gcacctatcg gtattcctca tacaaatatg
     6781 gggttattcc atacatccag ctcattgatt tctaaaccga tcttttgtat tttcaatttg
     6841 aaacatccgt tgaccattga agggatggat agacagccag tcgatttgtc ccgtatgtta
     6901 gttatgctgg cgccttcgcc aatccaagaa aaagacggga ttcttttagg aaagatcagt
     6961 ggagcaatca tcatgaatga cttgaacacc gagatcttcc gctcaggaaa taaagaaatc
     7021 gtttatcagt tgttagcgaa aatcctgatt gaagaaataa agaaatgaag gtgaaaagat
     7081 gatacagcta ccagcagaaa aaatattgct cgatcaatcc tatcaaaatt ggaaagaagc
     7141 gttgaccgag atcggccagt ggatgaccca acaaggggga atcaaaccgg catatatcca
     7201 atcaatgatc gaaagacaag aaaaagcgag tgtctatatc gggaatttcg tagccttgcc
     7261 acatgggact gacaaagatg aagaagtagt agaagaagga atctatttag tccaagtgcc
     7321 tgatggtgtg gactttggaa caaaagagga accaaaaatc gcgacactct tatttgtagt
     7381 ggctttaaaa aaagaacatc agttagcggt gctccaagat ttagcctttt tctgttcaga
     7441 catcgaccaa gtaatggcgc tcagtgatgc gaaaaccatt gaagaagtcc aaatgatttt
     7501 tgaacatgga catatataac caatgttcac agtaaaaaaa gctgccttgc atcaagtaat
     7561 aacttcaacc aagataaaaa taagaggagc tatttttgtt gggaggttct tattactgcg
     7621 gatgcaagct tttgaaaact atttattcta gaaaaccgtt tatttgaaat aggggacggt
     7681 gacaaggttt ttgtcaccgc ccctattatt tttgtgtttt aatagctctt ttttatatag
     7741 atagtgccca tacctatgaa taataaaacg atactccata taccaagtgt caagatacca
     7801 ttcatgaaag taacctgttc tgaagctagt ggcgagaaaa tatctcctcc tatgatcaca
     7861 ttttgaaaat caacataaga agttggcaag taatgactga ttgattctat ccatccaatc
     7921 gaagaaaaca agcgatactc tgctaagaaa agacaaagaa gtgtacctaa aagattgact
     7981 agagaatgtt caaataaagc agatagaaga aaactgattg tacttaaaaa gagcacccac
     8041 aacaacagga acagaaagct ctggaaaaga tactcataca tcggaaggat tcgtatctcc
     8101 tgaccaatga tcgttcctac aggataacgg aagtctccca aaccattttt gattattact
     8161 agtatagaga cgatcaaaaa tggcatcacg agacttagca tagctgcacc ataataaatg
     8221 gtaaaacgga tgctatgttt ctttaccata ctatccggta atagattgac tagagagatt
     8281 acctgctttc ttttttcata agagatgatg gaagaaagaa acaaggcggt tatacataaa
     8341 atcaaaaaac tagaaatcat tcctgaaaaa atattttcgt agtaatttgc taaagggaga
     8401 tgcgctggca agtcaaaaat agaatatctt tggatctgat gttctttcat ataacggagc
     8461 aactcgacac gtttttgttg ttcaatgatt ggaattcctt gtctttgacc agaaatcagt
     8521 cgatccagta tatctttttc ataatgataa gttgcttcga ccatttgctg gacattccct
     8581 gtgttttttg catgtaagat tgcttcgatc agtttgttgc ttttctctaa actttccaca
     8641 aattctttct cattctcatc ttctttcaac gtttcaatca aacgttgcat ctcttcatag
     8701 ttatgattca gttcatcgaa ttctgtataa gcaagtttat gatcaaaaaa aagtgggaat
     8761 gcaaagagca cgaccaacaa tagaggaaat aaaaagttta ctttgttttt tagtaagaga
     8821 ttgcgttcaa atgtaagtaa agacatagtt acacctcata taatttacga taaatagcta
     8881 aaatctcttc catatttgag gattctgtga cgatttctcc attttttaga aaacatgctt
     8941 tagaacaaat cgattggata ttcgcgatag cgtgggaaga aagaagaatc gtcttcccct
     9001 ctttctgcca ttgtctgatg aggctattca agatatcaat acttcctggg tctaatccgt
     9061 tcaacggttc atcgaataac atgacagatg aatcactgat caagtaagca gcaagcaaga
     9121 catgttgttt cattcctaga gacatttttc caatcggcaa agtaaggtac tcagtcatct
     9181 tcaatcgttg gaaaacagtt tggcattcta ctttgctctg ccaatagtat ttgatcaaag
     9241 tcaaataatc aacagccgat agatagtcgg ataactgatt cgttgattct agaaaaaaga
     9301 aagaccggcg ataatctgta taagcagtag gagagatgtt gtgaagggac aattgatcat
     9361 aagttgttgg cagcagttgg gcaatgccat tcagcaaagt tgttttgcca aatccgttcg
     9421 gggcaatcaa agcagctact tctcctgatt ctactaaaag attcaattgt cgaatcacac
     9481 gttttttacc acgtttcatg ttcatgtttt tgatcagtaa tgtcatagat aggcctccaa
     9541 tataaaattt aataacataa tagaatggaa aacatctaaa ttaaatgcta acaaaagtta
     9601 ttgtttgcat attataaaaa aattaaaata acagttttat ttaaaattga agttttctga
     9661 taccgaaatc tttttatttt gctgacatac tcaaaccttt ggcacaaagt ttcgtgccaa
     9721 agtacgacta tttctctttt tagtaagcgg atacaattag agtatcaaaa ggagagaggg
     9781 agagacaaaa tggaacggac gattcccaaa aacaacattt cgctaaaagc gagggtacaa
     9841 aaattaggca gtactttatc aagcatggtc atgccaaata ttggagcact gattgcctgg
     9901 ggtgtattaa cggctctatt tatcccagat ggttatttac caaatgaggc tttggctacc
     9961 atggtaagcc caatgctaac ttatctgatt cctttattga tcggttatac tggcggaaaa
    10021 gtaattgctg gtgaccgcgg atcagttgtt ggggcaattg caacgatggg tgtgattgtc
    10081 ggaacagata ttccaatgat gctaggcgcg atgatcatgg gacctcttgg tggatatgcc
    10141 atcaagaaat ttgatcagct attccaaaaa cggatcaaat ccggttttga aatgctagtc
    10201 aataattttt cagcaggtct tatcggtttt ggattagcgt tattaggttt ttctgcgatt
    10261 ggtccagttg tcgatgcatt gactcaagca atggcaaaag gtgtagagat cattttatct
    10321 gcccatttga ttccattgac aagtattttt attgaaccag caaaaatctt atttttgaat
    10381 aatgcaatca atcatgggat tttgacacca ttgggaactg aacaagtagc agaggctgga
    10441 aaatctattc tattcttgct tgaagcaaat cctggtccag gacttggcgt attacttgct
    10501 tttactttgt ttggcaaagg agcagcaaaa tcttcagcac caggagccat gatcattcac
    10561 tttttaggcg ggatccatga aatctatttt ccttacgtta tgatgaaacc gttattattc
    10621 ttatcagtta taggcggagg aatgtcagga agtttcattt tccaattgat ggatgctgga
    10681 ctaagagcgc cagcttctcc agggtcgatc attgcgattc tagcgatgac accgttcaat
    10741 tcttaccttc ctgttatttt gggcgtgatt gttgccgcag ctgtttcgtt tggtatttct
    10801 gcagtaattt tgaaagcgga tgcgaaagat acaaatgaag acttcgaaaa atctgttcaa
    10861 gagacaaagg cagctaagca agcttccaaa ggtgcccaag gtattcctca aggagctaca
    10921 ttctctggcg tacaaaaaat catttttgct tgtgatgcag gaatgggatc aagtgcgatg
    10981 ggcgcttcga tcttgagaaa aaaagtacag gaagcagact tgcctcaaac ggtgaccaat
    11041 caagcaatca gccaactgac agatgactcg aatacattga tcgttactca ggtcgaacta
    11101 caagaaagag caaaacaaaa agcaccgagc gcgcgttttg tctcagtaga aaactttttg
    11161 aattcacctc gatatgatga aatcattcaa gaattaagca gcaagcagaa aacatcagtt
    11221 tctgaaattg agaatgagcc agcagaagag tttctttcag caaacatcaa acaaatcaaa
    11281 gagattctac ttgtccacga tgatcgttcc ggatcagcaa caatggggat gaccgtgctc
    11341 aaagatatat tagacaagaa ccatttaact atcccgttaa gaaaagtcaa tatccaagat
    11401 ttaaaagatg aacctgattc actgatcgta gccaaagaaa gtttagcgga aaaagtacag
    11461 atggctgcac ctcaatccat tcatttatca gtcaacagca tggtcaatcc gcaaaaatac
    11521 gaatcgattg cacaatattt gaaacagaca gcttaaggga ggaaaacagc atgcaaagtt
    11581 tgccattcga catgatcgag ctcaatcgat cttttaaaac caaagaagaa gcgatccgct
    11641 attgcggtca gatgcttgta gaagcagggg gcgttgatga ggaatatatc gatgccatgg
    11701 tagatagaga caaaatgtta tctgtttata tggggaattt tatcgcgatt cctcatggaa
    11761 cagatgaagc aaaaaaatat gtgaaaaaat ccggcatctg tgtcgttcaa gtaccagatg
    11821 gtgtagattt tggtaccgaa gaagaagaaa agattgcgac tgttttgttc ggtattgcag
    11881 gagtcggaga tgagcatttg caacttgtac agcaaatcgc actttattgc agtgatatgg
    11941 acaatgttgt ccagctagca gacgcgctca gcaaagaaga agtaacggaa aatctagcaa
    12001 ttgcttaagg agagagaaaa atgagagcag tacattttgg tgcaggaaat atcggtcgag
    12061 gatttattgg agaggttttg gcagctaatg gatttgagat tactttcgta gatgtgaatc
    12121 acacgatcat tgatgcttta aatgaaagaa aagaatacga aatcgaatta gcagatgaat
    12181 caaaagaaaa aatcactgta aaaaatgtca aaggaatcaa caatcaaaca aatccagaaa
    12241 aagtaatcca agaaatcgct gaagcagatt tagttactac cgccattggc ccgaatatct
    12301 taccttttat tgctgagttg attgcaaaag gtatccaaga aagagaaaag gaaggaagca
    12361 caacatcttt agatatcatt gcctgtgaaa atatgattgg cggttcagca tttctagaaa
    12421 aagaagtgta taaatatctt ccggaaacat catttactga caaatatatc ggctttccaa
    12481 atgcagcagt cgatcgaatc gtcccattac aacaccatga agatccttta ttcgtccaag
    12541 tggaaccttt caaagaatgg gtcattgacg atagccaaag aaaaaataaa aaaatccaat
    12601 taaaaggggt tttatatgta gatgatctag aaccatatat cgaacgcaaa ctatttagtg
    12661 tcaatactgg tcatgcgact gtcgcttata caggtgcctt gttaggttac cagaccattg
    12721 atgaagcaat gcaagatgcg ttagttgtgg cacaattaaa atcagttttg caggaaacag
    12781 gaagtttgct gattgccaaa tgggggtttg atgcagagca gcatcatgcg tatattgaaa
    12841 aaatcattca tcgcttccaa aacaaaaata tctctgatgc gatcacacgt gtcgctcgga
    12901 caccattacg taagttaggt tatcaagaaa gatttacacg tccagtgaga gaactacaag
    12961 aacataactt gacttgccca catttgacgg caacaatggg gattattttt aattactatg
    13021 atcctgaaga tgaacaaagc cgtcaattgc atgaaatgaa gatccatgaa aacttggaac
    13081 aattgattca agaagtcaca ggaatcaatg atccgaaaac aatcgggaat atcaagcaaa
    13141 acgtgaaccg ttatgccaag caagtcgctt aaaataggat acgaaaaagg ggaatgtgac
    13201 gaaattttt
//
LOCUS       DAZKOB010000074        13192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000074 DAZKOB010000000
VERSION     DAZKOB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.074"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACG8M6_002036"
     CDS             complement(105..629)
                     /locus_tag="ACG8M6_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0457689.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8M6_002037"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACG8M6_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0457690.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8M6_002038"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACG8M6_002038"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0457691.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACG8M6_002039"
     CDS             complement(2351..3031)
                     /locus_tag="ACG8M6_002039"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0457692.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACG8M6_002040"
     CDS             3157..4026
                     /locus_tag="ACG8M6_002040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0457693.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACG8M6_002041"
     CDS             4052..4567
                     /locus_tag="ACG8M6_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0457694.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACG8M6_002042"
     CDS             complement(4614..5177)
                     /locus_tag="ACG8M6_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0457695.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACG8M6_002043"
     CDS             complement(5232..5411)
                     /locus_tag="ACG8M6_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457696.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACG8M6_002044"
     CDS             complement(5488..5997)
                     /locus_tag="ACG8M6_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457697.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACG8M6_002045"
     CDS             complement(6093..6473)
                     /locus_tag="ACG8M6_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0457698.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8M6_002046"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACG8M6_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0457699.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7873..9090)
                     /locus_tag="ACG8M6_002047"
     CDS             complement(7873..9090)
                     /locus_tag="ACG8M6_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0457700.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9348..10625)
                     /locus_tag="ACG8M6_002048"
     CDS             complement(9348..10625)
                     /locus_tag="ACG8M6_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457701.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="ACG8M6_002049"
     CDS             complement(10704..11348)
                     /gene="pcp"
                     /locus_tag="ACG8M6_002049"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0457702.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11547..12491)
                     /locus_tag="ACG8M6_002050"
     CDS             complement(11547..12491)
                     /locus_tag="ACG8M6_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0457703.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12493..13179)
                     /locus_tag="ACG8M6_002051"
     CDS             complement(12493..13179)
                     /locus_tag="ACG8M6_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0457704.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3735 a         2764 c         2390 g         4303 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct atttcctacg
     7741 attagtcaag taaaaggtgc aagaaaaata gctcgtaaac gaaagcgaca aaatcaaagc
     7801 acaccaaata aagccccgtt cgtaaaaaat tttcgatctg ggcaaagaag tggtatactg
     7861 taagtggttt agttaatggt ccactgagac cgcgtacgca tagtcgtact ctgtatgtgc
     7921 cctaaccata gcgtgcatac atttcagaag cttgttcata caagcaactg tggcaacctt
     7981 atctttctta gggataggtt gcttttttaa tttgtagtaa taatcgacaa tatgattagg
     8041 agctgcgcgt tgctggcgaa tcatatttcg aatggtgaag aaaataattt tccgtgcttt
     8101 gggattacca cgcttattga tgcggtcttg gccagtatac ttacctgaat gataccgtcg
     8161 gatgtcgatt ccgacaaagg cattcaattg tttatgggtt ttaaatcgtg tgatatctcc
     8221 taattcaccg agcaataaag cggctgtgag tgcgccaatc ccagggaacg tttggtacaa
     8281 caaaaagcaa ggagagcctt cggctttttt gatcatttgt gtcgccagtg cttttttttg
     8341 ttcgagtaaa tcttggagaa gttcggcgta atagatcgtt ttttgacagt ggatactgtc
     8401 tttttcaaca gctggatagg cacactgtgc atagtggatg atttgatctg ccttttgctc
     8461 ggcacgattc gcggagattt ttttccgtgt ttcgttgatc agtttatttt ttatttttgt
     8521 ccgagtagat gccaatacaa aatctggatg gggaaaaaga cggatcaatg tcaatgcgta
     8581 aggggtcagc ctgtttgaaa agaactgttc aagctctgga aaagtcaatt gaaggcagtt
     8641 gtgtaaatcc atgcgcagac gagtgatttt cttctcaatt tcttgataaa aacgggctaa
     8701 atcgcgcatc tctaagtaaa aattgctttg tacttctttt ggctgacgtt gatttttaag
     8761 atgagtctgt gcgagccggt gggcatccga tttatctgtt ttccaactgc gtaaggtgct
     8821 ttcttccatc tgttttttcg cctctaacgg gtttaacaaa cagtaagaaa agtgatgatc
     8881 ttgaaagaac ttttcgactg gtctggaata aatccccgtt gcttcaaaaa caatcaatgg
     8941 acgctcaggc aagtgctgga tctcttcgag taatgcatga aatcctcttt gagtgtgttc
     9001 aatctcgtct tcaaataagc aggtgtcgtc tttgtatagg acactgtagc tgtgtcccat
     9061 tgaaacatct aatgcaagaa tgtaaggcat ttgttttcct cctttttaag ttttgaaaga
     9121 actttactgc ttcatttcga ttccaatttc ctatacacgg gctcatggcc ccaacatacg
     9181 taaacctgat tctaaaatga cagtaaagaa gttcggtttt tgatagggac tcgtagtcct
     9241 caaggctttg ttcgaacttt ctttcactct tcactataat ctaaaaatag aaaatagagg
     9301 agaacatcat tcgtcaatga tgttcccctc taatcttagt atgtttttta tagtaattcc
     9361 ttcagatact ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat
     9421 tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt
     9481 gtctcttgca catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag
     9541 actgtacggt ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt
     9601 aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag
     9661 cgacaatatt gcttccaaat aaatagatag atttttggtt ggatgaatcg ttggtttttc
     9721 gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac
     9781 acggaaataa atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg
     9841 gattgccaat ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt
     9901 actaattctt ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc
     9961 ccattgatgt gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg
    10021 taaatcggca tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt
    10081 aaatatggct gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt
    10141 ccttcaggaa aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca
    10201 acttttgtca ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg
    10261 gcaaagtggt tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat
    10321 tgcactttta attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga
    10381 aaaaacggat tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct
    10441 tccataaaac gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt
    10501 ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg
    10561 atgggatagc ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct
    10621 tccacggctg tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca
    10681 accagccagt gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct
    10741 ttattttcta ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac
    10801 atagaaggcg tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg
    10861 aacccacctc gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata
    10921 tcattgcaga caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt
    10981 acattttttg tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct
    11041 tctaaagccg tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga
    11101 ttgattgcga cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc
    11161 acgacatctg gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat
    11221 acggtcggta cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt
    11281 actgcttcat aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt
    11341 actttcatct gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc
    11401 attttccttg aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac
    11461 ttaatatatt cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat
    11521 tattctgtca caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa
    11581 actagcataa ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc
    11641 tccagcatcg ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca
    11701 caaaaaccgc aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat
    11761 acgaatggtg caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg
    11821 attaccgtaa atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct
    11881 tctggaacga tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat
    11941 accgcaccta gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc
    12001 atatctgctg catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag
    12061 attagcatgg ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat
    12121 agagcaaaca agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct
    12181 agtttgtctg acattttttc catatattct tttgatggaa gccggctttc tcctttgctg
    12241 actttcttag caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc
    12301 ggaagccatc ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca
    12361 taacgatgag gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt
    12421 cccatcacga tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta
    12481 aaaaagtcca tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt
    12541 aagatagaag tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa
    12601 agcaagctgt cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa
    12661 cgcccctgct cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc
    12721 tgaagccata ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc
    12781 gatggtcccc aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa
    12841 actcacgtta aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc
    12901 agcagatact ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct
    12961 ttcaagcgtt cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac
    13021 aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt
    13081 aacaactaaa gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat
    13141 aacaatcaag acgcctaaaa ggctaagata attttccata tattttcctc ca
//
LOCUS       DAZKOB010000075        13085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000075 DAZKOB010000000
VERSION     DAZKOB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.075"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..1788
                     /gene="yfcC"
                     /locus_tag="ACG8M6_002052"
     CDS             49..1788
                     /gene="yfcC"
                     /locus_tag="ACG8M6_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0457705.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1945..3129
                     /gene="iadA"
                     /locus_tag="ACG8M6_002053"
     CDS             1945..3129
                     /gene="iadA"
                     /locus_tag="ACG8M6_002053"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0457706.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3240..3443
                     /locus_tag="ACG8M6_002054"
     CDS             3240..3443
                     /locus_tag="ACG8M6_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0457707.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3544..4206)
                     /locus_tag="ACG8M6_002055"
     CDS             complement(3544..4206)
                     /locus_tag="ACG8M6_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457708.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4219..5721)
                     /locus_tag="ACG8M6_002056"
     CDS             complement(4219..5721)
                     /locus_tag="ACG8M6_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0457709.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6269..7948
                     /locus_tag="ACG8M6_002057"
     CDS             6269..7948
                     /locus_tag="ACG8M6_002057"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0457710.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8075..8554
                     /locus_tag="ACG8M6_002058"
     CDS             8075..8554
                     /locus_tag="ACG8M6_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0457711.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8773..9453
                     /locus_tag="ACG8M6_002059"
     CDS             8773..9453
                     /locus_tag="ACG8M6_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0457712.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9461..10792
                     /locus_tag="ACG8M6_002060"
     CDS             9461..10792
                     /locus_tag="ACG8M6_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0457713.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10807..11769
                     /locus_tag="ACG8M6_002061"
     CDS             10807..11769
                     /locus_tag="ACG8M6_002061"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0457714.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11880..12218
                     /locus_tag="ACG8M6_002062"
     CDS             11880..12218
                     /locus_tag="ACG8M6_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0457715.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12297..13004
                     /locus_tag="ACG8M6_002063"
     CDS             12297..13004
                     /locus_tag="ACG8M6_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0457716.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4157 a         2315 c         2875 g         3738 t
ORIGIN      
        1 cattttttat tcatcaaaat tactgaaaaa agaaaggaag aaataaattt gaagaaaaca
       61 aattcttcag gaaaaccgaa acgcgacttc actaaattat cgacacccca tacgtatgtc
      121 attatttttg gtgtagtcat ctttgcatgg atactgactt ttgtcgttcc agcaggaaag
      181 ttcagcacgc aagatattga atacaaagat gcaaatggcg aaacaagtac tcgtactgtt
      241 ttacgtcaag attccttccg ttatgcttac gagctggata agtcgtatgt gtttgatcaa
      301 ttagaagaac tgcaagatca tccagcagaa cgtgaaaaac tggatgttcc ggaaaaagga
      361 ttggaaaaag tcattgctga cggagaaaaa aatcttacgc aggaaaaact agatgaaatc
      421 tccttgacag atgatgtgct ttacgatcag tacggcgaaa atatctatga tacttctaaa
      481 aaattgcata aaacagccaa aatatgggga acagatgatt ttggcggctt tggtttctta
      541 aattttgttt ttgaaggact agtatctgga gataagtatg gatctgctgt agggatcgct
      601 gcattgatcc ttgttgttgg aggtgctttt ggtattatta tgcgtaccgg agcgatcgac
      661 gcaggtatct atgcgtttat cagtaaaact aagggactgg agcgtttagc tttaccgcta
      721 ttattctttg ctttttcatt cggtggcgct acattcggga tggcagaaga ggtaattcca
      781 ttctcaatgg tgatggttcc atttgtcatt gcgttaggat atgactcgat cgtagcagta
      841 accgtgactt atgtcgcatc tcaagtcgga aatgcgacct cttggatgag cccgtttagc
      901 gtagcggtgg ctcaagggat tgctggaatc cccgtgttgt ctggtgcgac attccgattg
      961 attatgtggg tagttgttac tgcattagcc gctggttata tgatgatata tgcagaaaaa
     1021 atccgtaaaa aaccagaaag ttcgttgact tacaaatcag atgattattt ccgttctcat
     1081 atcaaaaaga catctgatga aaataaaaaa ttcatgcttg ggcataaatt gatcttatta
     1141 gagatgttag ttgtccttgt ctggatcgtt tggggcgtga ctcaaaaagg ctattacatt
     1201 ccagaaatcg cttctcaatt ctttgtgatg ggattagttg ctggaatcat tgctgttctt
     1261 ttcaaattag atggaatgaa agtcaatgac atcgcttctt ctttccaatc tggtgcagct
     1321 gatttagctg gtacagcaat cgtagtaggt atggccaaag ggattcttct tgtcttggga
     1381 ggatctgatg catctgttcc ttcagctttg aatacgattt tgcatgggat cgggacggcg
     1441 ttgaccggtg ttccagctgt cattggtgct tgggcgatgt acatcttcca aagtttgttc
     1501 aacttggttg ttacatcaaa ttcaggacaa gcagctttga ctatgccaat catggctcca
     1561 ctggcagatt tagtaggtgt atccagacaa gtagcagttc ttgcttatca gcttggttca
     1621 ggttttatgg acgcttttac acccgtatca gccagcttga ttggtgtgct aggtgttgca
     1681 cggatcgaat gggcaaaatg ggcaagattc caaatcaaga tgcaaggatt cctgtttgtt
     1741 ttaggaacta ttttcatcat gattgccatt gcaataggtc tgcagtagta ctggagacct
     1801 gtaaataaaa aagtaatcca aacagtagat aaggactcca agaaccgtca ttttacggta
     1861 aaatcaaagg agtaaagatc ctatcgtttg gattattttt taagatccta tcatcagtta
     1921 tacttaaaga aagaggaaaa atgtatgaaa atcattcgac aaattgaagt gtttgcacca
     1981 gattacttag gaaaaatgga tgtactgatt gccggtggga agattttggc tgtagaagaa
     2041 cagctagaag gcggctacga aggagtggaa gtagaagaac tttcaggtga gggaaaagta
     2101 ctaaccccgg gtttcattga ctgtcatttc catattttag gtggaggagg agaaggcggt
     2161 tatcaaaatc gaacgccaga agtcacattg agccaattga ctacagctgg tgtaacgaca
     2221 gttgtcggtt gtttgggaac agacggagaa ggtagagata tgactgcgtt gatcagcaaa
     2281 gcgaaaggtc tggaagcaga aggaatctcc acttatgtat acgaaggttc gtaccgattg
     2341 ccggtaaaaa cagtaacaga ttctatcatc aaagactttt tgacaattga taaaattatc
     2401 ggaatcggag aaatcgcagt atccgatcac cgttcttctc agccaagttt tgaagagttc
     2461 gctcgtgcag tagcagatgc tcgtgtagga ggcatgttat ccggaaaagc tgggatcatc
     2521 aatgtccact taggcggagg aaaaagaaag ctagagctat tgacacgaat cgtagaagaa
     2581 acagaaattc cgatcacaca atttttgcct actcatgcaa atcgcacacc agaattattt
     2641 gaagcttgcg tggcatttgc caaacgtggt ggcacgattg acttcactgc tagcgaagat
     2701 cctgattttt gggagaagac agatggtgaa gttcgcttca gcaaagcact gaaacgattg
     2761 atcgaggaag atgtttcttt agataatttc acgatgacct cagatggaca aggaagcttg
     2821 ccttattttg atgaaaacaa tcatttccta gggttaggag taggaagtgc caaggcattg
     2881 ctagtaggga tcaaagaagc tgtgcaaaaa gaaagtatcc ctttggaaat cgccttacga
     2941 gctataacca gtaatccagc acgtatattg aaattggata aaaaaggaaa aatcgaaatt
     3001 ggtgctgatg cagatctgtg tatcttggat aaagaaacgc ttgatattga tacagttatc
     3061 gctaaaggag aaataatggt ccaagaaaaa gaagtcaaag tatgggggac tttcgaaaaa
     3121 agtttttaat caacagctct aactatcttt tttagaaaac gagcgttctg tttgtctgta
     3181 tctgttgttg atgttaccct gaaggtagat caataaacaa actaagagga ggaagcacaa
     3241 tggcagattt aaaaggacgt tctaaagata tgaaagataa agtagtcggt gaaggaaaag
     3301 aagcatacgg caaagcaaca gacgacaaga gcaaagaaac agaagggaaa gcacaatcac
     3361 taggtgctga catcaaagaa aaagctcgaa atttaggcga cgaagtcaaa gacaagtttt
     3421 ctcgaaaaga cgaaaaaaaa taaaaaccac ttattactaa ttgaatcaag caaataaaaa
     3481 atccgaacca tatctgcgag acagatatgg ttcggatttt ttatttgatc attatcctgt
     3541 tttttaaact tcgatttttt gaaatttttt attttttagt tcaaagactt catcacagct
     3601 ttttgctact tcaatctcat gagtcacgat gataatacag cgattttgtt catgggcgat
     3661 ttcttggaaa agtttgacga tatcttgtga agttgtttca tctagatttc cagtaggctc
     3721 atccgctacg attagttgat gttcacagca gatagcacga acgatagcga ctcgctgctg
     3781 ctgacctccc gaaagtttgg tcacctgttt ttttgctaat atttcatcaa ttcctacacg
     3841 agccagtcga tctaaagcaa cttgtcgata attctcttgc ttggtcttag tgatttccat
     3901 tgctgctatg acgttgtcta gagccgataa ataaggaagc aagttgtaag cttggaaaat
     3961 aatcgagaca tcatttttac gataacttct taacccgatt ttgttcagtg aacgatcttg
     4021 atagtatacc tttccttctt ttggtgcatc tagtccagaa ataagcgaaa gaaaagtcgt
     4081 ttttccagaa ccacttgctc ctaaaatagc gtacattttt ccagcttcga aattcagttc
     4141 tacatcttta taaagcgcat cttgcggatc ttgataccaa aatcccaatt gttctgtccg
     4201 taacatcaaa ttcctccttt agtttaatag gatttttctt gggtttaatc ttaatatccc
     4261 tatagaagct aaaaagacag aaaacagact gattccaaat gctactccgg ctaatagtcc
     4321 caattgtgca ggtttgacat tgatttccaa ttcgctgact tgatcagaaa cagcaaatgg
     4381 attattcgtg gttccttgtt gaccatcgcg ttgaccgcct tgttcttgtc tgttacctgg
     4441 catttgtcca ccgccaggag cttgctggtt ttgtgtcgtg gtttctgtct gttggtcaag
     4501 tagctgctgt cccacggcat tcgcgacgac gtttccgcta agcgaagcaa ctcctaatgc
     4561 aagaatcata caaatagcca cttcggtaaa caattggaga atgattttac ttctggattc
     4621 tcctaaagat agcaagacgc ctaactcatg ccgacgctcc cgaatggtaa tcataatgat
     4681 cagagtaagt atgataatac cggctacagc cactagtaaa acaacatttt tagaaaaact
     4741 tgccacgttg tttaaaggtt ccaacattga ctgatacatg gaatcatttg attgtaagct
     4801 aaatgtatct gtgtcgatca gcttctcagc ttctttgaga aactcatcca ttttatctgg
     4861 atcagataga gtgtaaatag cagaatctat cgtgttatcg tcagatgtcc catttagttc
     4921 atttgcaaaa gtgtaagaag tgtaaagtgt attcgacgga ttcatgaagt tgaatttcat
     4981 acccatactg ccgcttgttt ctctggattc ataaattcct ttgatcgtca tttcataagt
     5041 ggtatcttca tcttcagtac tcgtgattgt gaaagtatct ccgactgcca ggtcatttgt
     5101 ttctgctaga gtagaatcaa tgactacatt gtttgtgcct ttgtcgctgt catctattcc
     5161 ttcaccatcc gtgatttttg ctgttccatc agaaaaatta ctattttgtg cagttgctga
     5221 cacaccactg atttgaaagt ctgcttgagt catttgcgga ttcccgccca ttcctccagc
     5281 aggttcaccg cctgcatcag tagtttctga actgtcttca cttgaatcag aactagtgat
     5341 ggcagttatt ccatcttttg ccatagctga ggcggaactt tcaaaggaat aactctttac
     5401 attatctaat tcagctattt tttccgcgtc cgttaaggaa actggtgtaa gagaaaaact
     5461 gcctggatct ggacgttctt cagtggtaga accgcttgaa gaagtttgtt cctgtttttt
     5521 aaatgaagct tctcgattgg cacttaaagt cactgttgct ccaacttctt tttgtgcttg
     5581 attcgctgct tcttctgcag cgcttcgaat cgtgaggcca gctagcacaa aaataagaat
     5641 ggctgtgaac acggcaaaca ataacaatga acgtccccat tttacttttg tacttttcca
     5701 tgcacgtttc ataaaattca tattttgcat ccctcctagt agatgcattt gataatagac
     5761 aacctatctt aaagctagct taaatcttat gctgtttgca caccatcaac atgaggttct
     5821 gtatatataa aaagtcacta tagttattgc aaaaaatgtg gtaaaatgaa aaaagtatgt
     5881 ttcaaaccga cgaagaagaa atgaaagagg aatagcaaaa gaaatcctag atgctacaga
     5941 gggattttta cgtgcatagt tgttctaaca attgctgata ctagcgattt agtttatttg
     6001 ttgtataata atggggaaca gctgaatact tatataagta ttctggctat agcgtttcgc
     6061 aaaagatcca ttttgttaat gaagtatatc caaaataaaa tgcccatcca actgtcagaa
     6121 cgcggatgtg ctggtaattt tcctgctgat tctccataag ttgagaaata gtgattttgc
     6181 atatgcttca ttggaatggc tcatatattt agataaagaa gaaaatcgac atctttaaac
     6241 ttggatgtca taagaagagg tgaagtttgt gagtaccatt aaaattatcc cattaggcgg
     6301 cgttcgtgaa aatggaaaaa acatgtatgt cgcagaagtg ggcgaggata tttttatttt
     6361 agattgtggg ctaaaatatc ctgaaaatga gttgttgggg atcgacgtcg taatccctga
     6421 ttttacgtat ttagaagaaa acattgatcg tgtagctggt gtctttttaa cacatggaca
     6481 tgcagatgcg atcggggcac ttccttactt gttgtcaaaa atttctgtac cagtattcgg
     6541 gacagagttg acgatcgagt tagcaaaatt gaatgtcagt cgtaatgaag cagccaaagg
     6601 atttaaggat ttccatgtgg tagatgagca tacagaaata gactttggtg aaacagtagt
     6661 gagctttttc cgtaccactc atacgatacc agattcagtg gggatcaatc tgaaaacaaa
     6721 agaaggtagt atcgtttata cgggagattt taaatttgac caaacggcta tcccaatgta
     6781 tcaaacagat tatgcacgat tggcggaaat cggcaaagaa ggagtcttag ccttactaag
     6841 tgaatcggca aatgcagaaa atcctcagcc tgtagcttct gaagcccaaa tagcagatga
     6901 agtatttgat acgatcaaat attgggaagg gcgtatcatc gtcgcttgtg tagcaagtaa
     6961 tttgcaacga gtgcaacaag tattggatgc agcagaaaaa gcacaacgaa aagttgtctt
     7021 gactggtcaa gatttcgaac gaattattcg cacagcgatg cgtttagaaa aacttcaatt
     7081 accaagtgaa gatctgcttg ttacgttgaa agacatgaaa aactatgatc cagaagaact
     7141 gatcatttta gaaactggcc gaatgggtga acccatcaag tctctgcaaa aaatggcaaa
     7201 cggtacacac cgtaatgtca agattgaaga aggagatttg gtttatatca ctactacgcc
     7261 aagcatcgcg atggaaacca tcgtggcgaa aacggaagat atcatttatc gagctggtgg
     7321 aacagtgaag ttgatctctg agaatatgcg cgtttctggc catgccaatc ctaatgactt
     7381 gcaattaatg atcaatttga tgaaaccaac atattttgtg ccagttcaag gagagtatcg
     7441 tgaattagct gctcatgcag atttggctca tgcagtaggg atgccataca aaaatatcta
     7501 tattactgga cgcggagacg tgctggaata taagaacaag cgtatgagtg ttgcaggaac
     7561 tacaagtgca gaaaatgtaa tgattgatgg attaggcata ggtgatatcg gaaacattgt
     7621 tttacgtgat cgaaaaatct tgtcagaaga cgggattttc gtagcggttg tcactatcag
     7681 tcggcgagaa aagaaaatca tttcaaaacc tcaaattact tcacggggat tcgtttacgt
     7741 aaaagctagc cgtgatctga tgaaagaaag cagcaatatg attgaaacaa tcgttgagaa
     7801 acatttagaa agcaatgatt ttgagtggag caaattaaag caagacatcc gagatcaatt
     7861 aggccgttac ttatttgaac aaacaaaacg ccgcccagtg atcttgccag tcattatgga
     7921 agctactcaa cgtcgaaata aaaaataaac aaaaagagta agatgaacct ctctatagag
     7981 tgctcgtctt gctctttttt tgcgaaaaac aaataatttt tgaatacagc atccctcgct
     8041 ttataataaa cgatagaata aagggagtga atcaatgatg agtcaaacaa aagtcgaacg
     8101 ttatttagaa gaacaaaaga tcagctatca accacttgat tttagtcatg tggtagaaga
     8161 atctttcaca gaagaactac aaaaaagagg gattgatcca gctttgatat gtaaaactct
     8221 cgtagtgaaa ggaaacaaaa ctggggtgat catcgcattg attcctttga acgatcactt
     8281 agattacaaa aaaacgagac aagtgacgaa agatcgtaaa gttggttttc ctggaatgga
     8341 ttttgtatta atgcatacag gatatcccca cggagcaaac acccctattg gaataaaact
     8401 cgcacatcct gattattgtt ttttagcaga cagttcttta aaagaaaaag aagaagtaat
     8461 tgtgtcaagc ggtgaaatcg ggagaagcct tcggataaaa gtagccgatt tagaaaagat
     8521 cgtccatcct atttacgctg atttaattag ttaagggacg agaagagaag ctaagcagag
     8581 cagagacctc aaacttgaga ttatatctgc ttggtacttg gtgcatcctg tattatctgt
     8641 tacaattgct ttgtacatac tgtactatca atccaatgaa aatatattta gcagctatat
     8701 tttttcattg atgtgcaagt gatgaaatcg tttttaataa aaaagataca gagaaaacgc
     8761 gaggtgtaaa atatgaaaat tacgtatcat ggacattcct gtttaacaat tgaaatggaa
     8821 aatggccaac gcgtgatgat tgacccgttt attacaggaa atccattgac agacttaact
     8881 gcagaagaag taaagacaga ttggttgctg attactcatg gtcatagtga ccacatagga
     8941 gatatgattc ctattgcaaa agaaaatcaa gccacaatta tcagtattgt tgagattgct
     9001 gattatgcga acagcagagg agtagattca ttcggtatga atatcggtgg aaaacatgcg
     9061 tttccattcg gcgtggtaaa attcgtacat gcacagcata gttcaagcta tgaagtagac
     9121 ggtatcgttc agtatatggg cgagcctagc ggaattatta ttcaagcaga aggaaaaacg
     9181 atttatcatg caggggatac tgcttatttc agtgatctag gattgcttgc agaagaattc
     9241 gatattgatg tcgctttctt gccgattggc gataactata cgatggggcc agaagatgca
     9301 gcaaaggctg cgcaaacaat tcgtgccaag aaagtagttc caatccatta caatactttc
     9361 cctgtcattg aacaagatcc cgaatctttt gtagagttac tggaagatag tgaaggaaaa
     9421 ataatgagcg tctcggaaac gctggaagtg taggcagaaa atggcgacaa aacatgattt
     9481 gattttagaa tatattgaaa gtttacctgt tggcaatcga atttctgtaa gaagcatcgc
     9541 aaaagaattg aatgtcagtg aaggaacagc gtaccgtgca atcaaagatg cagaaaatgt
     9601 cggactagtt tcaacaattc aacgagtagg gaccatccgt atcgaacgca aattaaaaaa
     9661 acatattgaa cgtttgacat ttggcgaggt cgtcaggatc attgaagggg acgttttagg
     9721 cggggcggca ggattagaca aggtcttgaa taaattcgtc atcggcgcga tgactaaaca
     9781 tgccatggaa cgctatatta cgccaggatc actaatgatc gtcggcaacc gggcggaagt
     9841 ccaaaaatta gcattggaag atggcgccgc agtattgatc acaggaggat tcaatacgac
     9901 tgctgagatt gcaaagctag ctgatgatct tgaaatgccg atcttacgca cctcttatga
     9961 tacatttaca gtcgcaacca tgatcaaccg agcgttaagc gaccaattga tcaaaaaaga
    10021 tatcatgctt gtcagtgata tctatatgcc agctgaaaag actcattatt tgcagcagac
    10081 agatacgatt tctgattatc agcgtttgtc agaagaaacg cagcattcgc gttttccagt
    10141 cgtcaaccgc catcatcgtt tgatggggat tgttacggca aaagatgttt tagggaaatc
    10201 gccaaaccag atcatcgatc gggtgatgac gaaagaacca atcagtgtaa agaaaacaat
    10261 gagtattgcc tctgttagcc atcaaatgat ctgggatggg ctagaagtca tgccggtcgt
    10321 ttccgatgac ttgacattgg aaggcttgat tacccggcaa gatgtaatga aagcgatgca
    10381 gcttgtccag cgacaaccgc agattgcaga tacgatttcg gatcagatat ctggaaatat
    10441 ccatacgatt gatactgacc gtgaaggaaa tcgtttggaa aaccctaagt tcaaattcgt
    10501 agtagcacca caaatggtca atggcgttgg aaccatttct tttggcgtac ttagcgaaat
    10561 catttctgat gtcgcacaaa aaacaatggt gatgaaccaa aaacgaaata tcttgattga
    10621 acaagtgaac ctacattatt tgcggctgat ccaattggaa agcgaattag acattcgtcc
    10681 acgtgtattg gaaatcggca gacgttccgc taaactggat atcgaagtgt ttattgaaaa
    10741 caccatcgtt tccaaagcaa tcgttgtttg tcaagtaatg gaacgttctt agaaagcagg
    10801 agtgaaatga cagtacagca agaaatagta gatacaataa aaaaatacga taaaatcatt
    10861 atccacaggc atttgcgccc tgatcctgat gcattaggat cacaagtagg attagccgaa
    10921 ctattacggg aaagtttccc agaaaaagaa atacgtcaag taggtgaaac gattgaaggg
    10981 cttcgctttt tagcagaaat gcaagaagtt gaagatgtct tctaccaagg agcgctagtc
    11041 atcgtgactg atacagctaa ttcacccaga atcagtgatc aaagatatca gttggcagaa
    11101 aaactgatca aaatcgacca tcatcccaat gatgaccctt atggagatct tgtgtgggta
    11161 aatacaaaag caagtagctg tagtgagatg attgcagaat ttgctttgat gtttcctgaa
    11221 tctttaaaga tgaacgttaa ggctgcacgg ttactttatg caggaatcgt cggagataca
    11281 gggcgctttt tgtatccctc cacgacgtcc cgtacattag agattgcttc gatattgaga
    11341 aattatccat ttgatgcaag tgctttgaac agagagattg aacaaatgcc gatgaaagtc
    11401 gctaaacttt ccggttatct ttatcaaaat atccaggtgg atgaaaatgg tgcaggaaga
    11461 ataatccttc cgcaagaaat tcttgataaa tatggaatcg atgattcaga gacagcttcg
    11521 atcgtttcac ttcctggagt gattgacgaa gtgctggctt gggggatttt tgttcagcag
    11581 ccttcagggt attaccgcgt acgtttacgt tctaaggggc cgatcatcaa tgaattggct
    11641 aaacgccatc atggaggagg ccatccatta gcaagtggtg cgaatgcaaa agatttagca
    11701 gaagtagagg aaatctataa agagatccaa gatatatgcc aatcgtataa gagcagaaaa
    11761 acagaatgat acttcgttcg aaaacgtgcc ttttgtagta aaaatcacaa atgtatcttt
    11821 tcaagatctt ctttttccgt tacaatagtg ctcactaaag attttaaagg agaaagaaga
    11881 tgacattacc aaattgccca gaatgtggct cagagtacac atatgaagat cgaggcttgt
    11941 tgatttgccc agaatgcggg aacgaatgga acaaagaaga acaagaaact gtcatagaag
    12001 acggattagt ggtattagat gcaaatggaa accgtctgtt agagggcgat agcgtgacag
    12061 tgattaaaga cttgaaagta aaaggtgcgt caggtgccat taaacaagga acaaaagtca
    12121 agaacatccg tttagtagaa ggcgatcata atatcgattg caaggtagaa ggatttggac
    12181 cgatgaaact gaaatcggaa tttgtgaaaa agaattaaag aatattcaag gtcttttcac
    12241 attcttttcc tactactaat gataaaataa agtaaacgaa aaggagtgaa acgaaaatgt
    12301 atggttatgg aatgtatggt tttttagatc cgacatttct tttggtcatt cttggattag
    12361 ttatttccgg aattgcgtca gcgtacgtca atagtacttt ccgaaaatac gatcaagtga
    12421 gaagcaaaaa caatgtgact ggtacacaag cagcacaata tatattacaa agtcaacaga
    12481 taaataatgt cggtgttcaa caaattgccg gagatctcac agataactac aattcaggta
    12541 ataagatgtt gagtttgtct caagcaactg cacagtctac ttcagttgct gcgataggtg
    12601 tagcagctca tgaatgcgga catgcggtac aagatgcttc gggttatgtc ccattgaaga
    12661 ttcgtgcagc acttgttcca gcagctaact ttggttcgat gatttcgttc cccttgatta
    12721 tggtaggtgt attgcttagc tggaactcta cgttaatcaa tatcgggatt ttcgcttttt
    12781 ctttagcatt acttttccaa ttggtgacat taccagtcga atttaacgcg tctcggcgag
    12841 caatccaaat tttgagtgaa ggcggactac tgacggaaga agaagtaccg atggcaagac
    12901 acgtattatt tgcagcagca ctgacttatg ttgcagcagc gctttcgaca ttcctgcagc
    12961 tgttgcgctt gatcttattg ttcggcggca accgaagaga ttaaaataaa gaagttgtga
    13021 aatcagcgct ttgatctgag ctaataccga gagttagctg attgaatacc atttatttgg
    13081 aataa
//
LOCUS       DAZKOB010000076        12747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000076 DAZKOB010000000
VERSION     DAZKOB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.076"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8M6_002064"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8M6_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0457717.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8M6_002065"
     CDS             complement(323..892)
                     /locus_tag="ACG8M6_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0457718.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8M6_002066"
     CDS             1078..1980
                     /locus_tag="ACG8M6_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0457719.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8M6_002067"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8M6_002067"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0457720.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8M6_002068"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8M6_002068"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0457721.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8M6_002069"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG8M6_002069"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0457722.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG8M6_002070"
     CDS             8554..8739
                     /locus_tag="ACG8M6_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0457723.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG8M6_002071"
     CDS             complement(8801..9997)
                     /locus_tag="ACG8M6_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0457724.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG8M6_002072"
     CDS             complement(10167..11603)
                     /locus_tag="ACG8M6_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0457725.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACG8M6_002073"
     CDS             11896..12705
                     /locus_tag="ACG8M6_002073"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0457726.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAZKOB010000077        12617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000077 DAZKOB010000000
VERSION     DAZKOB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.077"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..316)
                     /locus_tag="ACG8M6_002074"
     CDS             complement(62..316)
                     /locus_tag="ACG8M6_002074"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0457727.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(720..1502)
                     /locus_tag="ACG8M6_002075"
     CDS             complement(720..1502)
                     /locus_tag="ACG8M6_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457728.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1567..2403)
                     /locus_tag="ACG8M6_002076"
     CDS             complement(1567..2403)
                     /locus_tag="ACG8M6_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0457729.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2421..3830)
                     /locus_tag="ACG8M6_002077"
     CDS             complement(2421..3830)
                     /locus_tag="ACG8M6_002077"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0457730.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3844..5667)
                     /locus_tag="ACG8M6_002078"
     CDS             complement(3844..5667)
                     /locus_tag="ACG8M6_002078"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0457731.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6510..6836)
                     /locus_tag="ACG8M6_002079"
     CDS             complement(6510..6836)
                     /locus_tag="ACG8M6_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457732.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6882..7244)
                     /locus_tag="ACG8M6_002080"
     CDS             complement(6882..7244)
                     /locus_tag="ACG8M6_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0457733.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7281..7495)
                     /locus_tag="ACG8M6_002081"
                     /pseudo
     CDS             complement(7281..7495)
                     /locus_tag="ACG8M6_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7623..7958
                     /locus_tag="ACG8M6_002082"
     CDS             7623..7958
                     /locus_tag="ACG8M6_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0457734.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(8034..10031)
                     /locus_tag="ACG8M6_002083"
     CDS             complement(8034..10031)
                     /locus_tag="ACG8M6_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457735.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(10129..10404)
                     /locus_tag="ACG8M6_002084"
     CDS             complement(10129..10404)
                     /locus_tag="ACG8M6_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457736.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10636..11370
                     /locus_tag="ACG8M6_002085"
     CDS             10636..11370
                     /locus_tag="ACG8M6_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0457737.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11409..12551
                     /locus_tag="ACG8M6_002086"
     CDS             11409..12551
                     /locus_tag="ACG8M6_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0457738.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         4092 a         2509 c         1913 g         4103 t
ORIGIN      
        1 cgacataaca aagaaacagt gtaatcctta caccattttt caataaattg aacaagcgaa
       61 tttaggtttt ccgagagaat atggctgcgg ctttttttaa gatttcaagt tcctcattta
      121 aacgagcatt ttcttttctc agttcagcta cttcttttcc agtcagtcct gtggactgat
      181 caggtaaata taaattcttc catttgtaaa tcgttgcttc agataagcca tattctttcg
      241 ctaaccctcg aacagaacgt ccagtttgat tcagttcaac aatcatttgt ttaaaatttt
      301 cttcatatcg ggtcataaaa attcatcctc tcgtagataa gtttaattta ttcttgtctc
      361 tacgaaaagc tgtctaagat ttgatactaa caccaagtta tctttagaac catttcagat
      421 acatatgaaa caattacata tgattttttc ataaaaaaac agtactgtct agtagtataa
      481 aatatacaaa tttcttaaaa attttttcac taaaatcact acctgaaaca gtctaaaaca
      541 cttatatatc aacgtttaat gaaactatct ccgtttcatt tcagatacaa ataaatatag
      601 tcgggctcca aatccgaaaa aaaatacccc cagtagctta tcctaagcta ctggagatgt
      661 ataagtacgg tcatataaca acggggtagg gtaaataaat gaaaaagatg gaagaatcat
      721 tattcaataa cttttttaaa tacttcgttg taatcagtta cctgtccgtt cttaaatacc
      781 acagaataat cttttttccc attttcaagt aaccgaagtg gaatgtcttt accaacttgc
      841 tctgtcactg cattactgat acctgttaga ttagctaata aatctgataa agattgagca
      901 tctgtagaaa tctctgtatc agtatttatc gtgattcctt tatcttcatt tacaagcttc
      961 caagtaggtg gtatagctgt gtatgtttcg atgaatgaat caattctttc atctatcgtc
     1021 ttcccagtat ctacttctgt ctcattactc ttagatgaat cgattctttc atccatcgtc
     1081 tgctccgtaa ctgcttctgt ctcattattc tcaccggaaa ctctcttcac acccaaactt
     1141 atatattgtc catctttagt gtgcatagtt aaaattccca cgcttttact aataaattca
     1201 ctataaccag ttctttcaat agagacttca atgtatttca cactatcata ctcaaaagaa
     1261 aaatagacat gtaaaagcat tttgttgttt acatctacat aataatcctt tgactctact
     1321 tgagaccttc ctatacggaa tacagcttta ctgtctgata tctctatacc agcatcctta
     1381 ttattaatct gccaatctcc aagtagctct tctggcaagc tagtagtttc ttgtgaacac
     1441 tttaccactt cactggtaga ggtcgaaaca ttcacctccg cactgttaga agaattttcc
     1501 aaggaatcag gagccttagc acaaccaaca agtctttata tattgtgcag ccgatatttt
     1561 cttcattcag tacacccttg aaatatgcag tgttagataa agcagttcgt cctgatttgc
     1621 tgaggcaccc atctcttctt gaacgtactc cttgattttc aaggccgccc gatactcttt
     1681 cggaaaacgc tgcttcactg aagccagcaa aaaatcatcc ttctccggca actcttcctt
     1741 cttaatcatt cgggcaataa aaaactgtaa atgcgtcaca aatcttaagt aaagcatcga
     1801 atctttatca accttttttt cgggaaacag atatcctagc atagccaaga tatcctttag
     1861 tgctcttacc gagagcatgg tttcttccat atttgcttgt tcactttggg catttacaat
     1921 gtgaaacgca atgtttccag cctcctcttc tggcaactga acattcattt ttttgttgat
     1981 caattggata gcatagcagc cgatctcaaa ttcttttggg taatactttt gaatttcaat
     2041 cagcaggcga ttttgaaaaa caatgccttt ttgactgcgt tcaatggcat gcttcaagtg
     2101 atcaaccaag gtaaaaaata gacttttatt cagcttaaag tcaaacttgg cgttggcata
     2161 atcgataatt cgcttgacgc aaataattag atcatccgga gcagcctcaa acagtttaat
     2221 gtaatccttt tttagagcat cagaaagtga atctaattta aatacttttt ggattttgtc
     2281 agccggaacc aaatcgcctt tctttagttg aaatccaatc cctttcccca tcaaaattac
     2341 ttctttgcct tgcttatcta agcaaagaat taaactgttg tttaacgttt taattactct
     2401 cattagattc accgttctct ctaagctaag ccatttcttg cgattacttt ttgataccaa
     2461 aagaagcttt ctttttttat tcgttttaat tctttaagat tctcatcggt tcggtcaaca
     2521 tatataaaac cataacgctt attgaagcct tgatgggtac taacaacatc aatcgctgac
     2581 caaggtaaat aaccaaaaac atctacacca tctgttaaag ctgcttgaat agccagaata
     2641 tgtttggcta gataatcaat tcgatcctgg tcattaattc gtctatcttc ctcaactatg
     2701 tcgggtgccc catagccatt ttcagtaatt aaaataggta aatcatagcg atcgtacaat
     2761 ttcctcaatg ttaatcgtag tccagtaggg tcaactaccc agccatatgg cgttttatct
     2821 acataaggat tttcagctgc ccggtagaca ccttcttctc ccaacatgat ctgttgatcc
     2881 cccgctctag cggaaacatc tgatccgtca tttttgctgg cagcgatggt ggcagttgaa
     2941 taataattaa tcgcaataaa atcgggttta ccggaggata aaattgcctg atcctctgcc
     3001 tcaattttag gatataatcc acgatcattc aaatagtttt caaacaaata attgtatttt
     3061 ccccgaactg ctacatctaa gaaagaccag cctctcaatg tttcccaatt atgggctgca
     3121 attgcatctt ctggactgga ggtagcttga tacatagccg ttaaattgat tgctggaccg
     3181 atttttcctt taagattcaa ctgatgatac aagtgaatta ctctagcttg agctaataaa
     3241 acatggtgat taatttgaaa gagttctttt tttgaaggca gctctccact tggtggcaaa
     3301 ccaatcgctc caggatgaag gatcatcgtg ttttgctcat taattgtcag ccaatagttc
     3361 accttgtcac catactcagt aagcaatacc tgtacatagt ccacaaaata atcgatagtt
     3421 gctcgattta accaaccgcc ctgctcctct agaaaataag gtaaatcgaa atggtacatg
     3481 gtgacaatcg gttcgattcc ctgattcagg cattcatcaa ttacttgatg ataaaaagaa
     3541 attccctttt gattggtttt attattttca atatccataa ttcgcgtcca agcgatggaa
     3601 aagcgaaaag cttttaagcc taattcttta aacagctgta tatcttcttt atagtgatgg
     3661 taaaaatccg aggcaacttc aaaattagct accccttctg tatgctgata cttgtcaata
     3721 atagacggtt gtttcccatc ctcctgatag gcaccctctg cctggtaggc actaatggag
     3781 gcaccccata aaaacttctc tggaaatgct gaaagctgtt tgtattgcac aaaaatcctc
     3841 tccttaacta atttgcatca atggttcgtg ttcctttaag cttccgcttg cttcactaat
     3901 tcctgcgtaa ttttcactgt tggtcactac cacaatcact gtatcatcgt agccagcgtt
     3961 tctaatggca gcgaaatcgg cgttgattaa taattgtcct gctgacacct gatctcctgc
     4021 ttttacaaat acttcaaagg gttccccttt taaattcacg gtgtcgatcc ccacatggat
     4081 caataattca ataccttctt tagaacgtag accaatcgca tgtttagatt ctaaaatagt
     4141 aatgacttct ccctcaacag gtgaataaat gctgttttct tctggatgaa tggctacgcc
     4201 ctttcccaat agtcccttag aaaagacagc atcgttcact tcagaaatag gacacacggt
     4261 tccttttacc ggattttcaa caatgactgc ttcggctatt gccgtttgct ctgattcatc
     4321 agaagtatcg tcaaatccaa ttagataagc aatgataaag gttgcaacaa aggcgattac
     4381 acacgtaata attgcgtgaa caatattcat tggattttct ccaataaagg ctggtaaggc
     4441 tgctaagcca ggggaaacaa aggcatatct gactaaaccg ctcaagccag catacaaacc
     4501 agcaattgca ccaccaatca tggaagcaac taatggtttt ttcagcttca aggttactcc
     4561 atagagtgaa ggctcagtaa ttcccattaa agcagtgatc ccagtggaca cagctagctg
     4621 cttcaactct ttgtttttag ttttcgcggc tacagcaaag gtagcacctg attgagcaat
     4681 attagaagcc agcattcctg gcccattgat catttcagaa cccgtactgc tcaattgcat
     4741 agtagcgatt ggcgtcattg cccaagctgt acccgtaacc accaaaaatg gctgcaaacc
     4801 acccatcaaa aatggaatta accaagctgc tttgccgtta atccattcag cccccattgc
     4861 tataagatcg ttcagcaacg ttccaaacgg tccaatgaca accaacgcta aaggccccat
     4921 aattaaaaat accagcatcg gcttagtaaa gaatttaata aacgaaggcg aaattcgatc
     4981 cgcaaagggc tctacatagg acatgatcca aacagtaaag ataatcggta aaactgatcc
     5041 cgcataatct gcagctaata ccggaattcc tagaaaagaa acggcatcgc cagtagaaaa
     5101 aagctgtgaa atatttggat gaagcaaaat gccagcaaca gtaatcgcca aaattgggtt
     5161 gacatcaaac tttatggctg ccccataggc taatagtact ggcataaaat aaaaggctgc
     5221 atcagcaata gtattcaaaa tgatgtaagt ttgactactg tctgatagta caccagtcaa
     5281 agtcaagacg gccaaaatgg cttttaccat cccggcacct gttatagctg gaatcatcgg
     5341 tgtaaaggtg gtagaaatga cactaatcaa tcttgaaata atactttctt gattgtctga
     5401 agaggggccc tttttaattg aagttcccac ttccttttga atttccttga aggctgtttg
     5461 gacttcattg ccaatcacca cttggtattg tccaccgcta tccaccacac taattacacc
     5521 aagtgttttt tctaaggctt tcgtatcggc tttcctttta tctttgagtt caaaacgcag
     5581 tctggtggca caatgggtta agctgaccac attttctttt cccccaacct gttgcaatac
     5641 atttcttcct acttctctgt aatccattat tctctctcct taaagcagta aaaaagacca
     5701 agtcaaacca cgccccaatc aacattgaag tgttagtttg tcttggtctt gcctgcataa
     5761 tcagtaacat gccaataacg ttattttgct gtaatccaat aatagcacat tatgatagcg
     5821 caatcaacag ggaatttaat tttttatctt ttcgtttgac cgtatagggt aatataacag
     5881 ttagatcaca tcaactaggg tatatctgcg tatacttact gctaaaataa gtatatctaa
     5941 tattaactat accagtaaaa ccattcctac ataaccagcg ggaaattccc aaaatcgtga
     6001 aagcatcaaa aatgcatata acggatcaca caacttcaac aattctgctc ttgggaaaaa
     6061 ttgatgaatt tggtgagtaa aggatggaat aaacagcatt ccaacaatat aggcaacaat
     6121 agtgatactt gtttttttgg taaaaatcag taagcaatag ccaatttcaa tcattccgag
     6181 atataaaggt atcgatagaa tgatttgggc tagtattctt aaaaattaac tagcaaatgt
     6241 ttaaactgat ttatttctaa tctacatgta gtaagtattt caattccacc aattatcaat
     6301 aaaaatacca aacatatcca taataatttt ttctttttca tttcttttat attgttataa
     6361 cagtgaataa cataaaaatt cccgacaatc attgtaatat ttttttgtca cctgtattag
     6421 tatttaattt ataggaaact ataaaaactc cctagtgaat ttacaccttt gggagttcct
     6481 gtttggacgg tatgcataat acatttaaat caatttaaca ttaacacctc agcatatggt
     6541 atctccacat atatacggtc tttctttgga tagtactcaa tatttatgtt gatagcactt
     6601 ccattgtcta atatagcttt agcccggtat gtgttatctc cattttcatg agtaatcgtc
     6661 acatcatcac atgatcgatt taagttgtac tgttcttgta aaatctgtgt taccaatggt
     6721 ttcgatgact tctctaatcc agatgtacct gtcatttttg aaaaaatgat acctataata
     6781 gccaagacaa cgataactat aatgataaac ttcttgttca tatttacttt attcaacttt
     6841 ttatctccta tctccaaaat aatttgctct tatccctctt atcagtacat tgacatccct
     6901 aaaagtacac caaagattgc tccagctacc cctgctatca tcaaaatcgt acccatttgt
     6961 tcatctttat ctcgatataa gtagcccata ataacagcaa cagcaccaaa tacaacagga
     7021 ataaatgcta acgctactac catagaaatc caaccaataa tcaaatacag cttccctgtc
     7081 tcttgtccat gtacgatttg ttccttcatt tcattaattg tctgctcagt acttttcttt
     7141 actgcgtctg ctccagttgt ctcatctgtt tcttgtagtg ctccgcaaaa ctggcaatac
     7201 ttactttcat ctgttatttc tttgccacat tttctacaat acatgtcttc tcctcctaaa
     7261 aacattcaat tgtttattag ttaactgttt actttattaa atacatcaga aaacagctca
     7321 ttcattggta cacttaatat ctcagaaagc atcttcattt gatagggttt aggaatcgtt
     7381 ctccctgttt gccacgaact tacacattgc tgtgtaacat tcattttttt gctaactccg
     7441 tttgagaaag tcctttttct tttctgtatc ggctgagatt acttactttt ttcatgattc
     7501 tcccctcctg ttttattata tacaacaata tgatgtattt caactcataa aatataaaaa
     7561 aacttcaaat accattacaa atacaacaac ttgttgtact atactgagat gggggtgtat
     7621 taatgaaaca gaaacaatat gcaaaggtca ttagagaaaa gcgaaaagct ctcgggtgga
     7681 cacaaaaaga attagctgaa aaaatcttct ctacacaaca ggcagtagca agatgggaaa
     7741 attctgtaac agagcctaac ctcgatagct taaccgctct ttctagagcc ctaggaaccc
     7801 ctgtaagcca ttttctagac aatgttgtcg tagattatga ggaagagttt ttggctctct
     7861 atcgttctct tagcactgaa gatgcagttc gaacaattga ctatatgaag ctattaaaaa
     7921 ggcaagaaaa tgaacgcaat cagctattga aagattgaaa aataaataac ccaccgaact
     7981 ttaacggtgg gtgtattgag atgaaataag attaagagct atggataacc tgtctagttt
     8041 aatacaattg ggtcttttct cttccttact ctccgtccat catctcttgt tacacttgta
     8101 aattgataaa gaggaactaa ttcaactcct gataatatca attcttttat tacttcatct
     8161 atccctgctc cttttgctag gtagactaaa aaactactac ttgctttcac attgtatagt
     8221 ctcttaatat ccttagaagc tcgttcaaga gtgaatcctt gtttaacaaa gtagcctctg
     8281 ttttctttta aagcattttc cacgctgtta ttcccaacat tacgtagacg ttcacaaaca
     8341 gtgttagtaa gttctttctc tgtcattaga tatgttcctt gccacgataa gccctgtaaa
     8401 acttcatgtg cttcatctaa aatcaattgt ttttccttgc taagttctga tcgggaattt
     8461 tgaggaaata ctcttttagc ctctttttcg ttaaaacata tcttaaccca ttcataagaa
     8521 aaatattgat atgtaaaatg aaatttttcc agaagtggaa ggatatcttc attcaatttt
     8581 tgaatcacag aagttgcatt aaaaacgtct aatcgataga cattgcttct cttatgatta
     8641 aagtttccta acgctttctc ttcctttaac ctatttaggt attcgagcat cttcaaagga
     8701 acatcttcat catctaattt ttttatcaat cctataaaag tcataagtga tagtaactta
     8761 ttacatttat ctatgtcata agttttacca tagtaaaaac tcattttttt agctaattgt
     8821 tcccccgaca aaatacaagt cacactttcc ttactatcat caaaaaaatt aacatcagaa
     8881 agcaagacat catagacacg tcgcatttcc tctagataat tccttattgt cctgaaaagt
     8941 ttgtctttca ttgattcaag agtgtcaaaa agtacttttg attcctcttt caattttcga
     9001 aaatgtgtag gatacaagtc taggttctct aatagccaat taaatgtttc tacccgactg
     9061 tctttcttta aaattagcat cacaactcca ataaaatttt cactgatatc acaattactg
     9121 gaatgacaat aaaaaatgga atgcccagtt ttactggtaa aaatactagc agagggatta
     9181 tgatcgttgt gaaacagaca tcttaagttt ttacttgact taagtaattt attcatatca
     9241 atggttaaca atctttcata aatctcatta aaagacatgt cggaggaatc aaacaggcag
     9301 tctttaaacc actgtttcat ttcttctttg tcgtcatttt ttatcaactt aacaaaatcg
     9361 gtttcattga ctagttcata ctctctccct gaatcacttt gacaattttt agaacatgtc
     9421 atggtaaaat caatcggaag ctcagtaata tctaacatgt tgtcttcatt aattattatc
     9481 actgcatctg acttatatcc tccaaaaaac attctattcg aagcatttac attgtcatca
     9541 cacccgggga tgttcttttt caagtattcg taaacagcaa ccattttacc atagtcagta
     9601 attgcttctt ttaatagaaa gacaatcttg tatttttcta gattttcaga gtaccgaata
     9661 cttttttgta tcgcaaacgc agaattatga actaaattga tagataaaac cttttctatg
     9721 cttaagtatc tactatcatc tttactcaaa tctactacct tccccttctc atctttaaca
     9781 gtgttatcga catccagaat aattactctg gtttccacaa aactctcctt gtcattgcct
     9841 ttattctttg tcttgcttgg aataaatgat tgtgatgaat ttagtatctc tacaaactca
     9901 tctcttgtaa cttctctata gttatctaaa cacctatctc ttacatcagt tttttctttt
     9961 tttgtaactg gcttctctaa taatttttct ttatctacat aaattggaat attaatcatt
    10021 gatgtttcca tttgtgtttc ctccttataa aattctgtta aagactaaaa catacagata
    10081 ataaaaacaa actctttatt actatatgtt ttagtcttat ttgtttactc aacctttata
    10141 ccgttccatg aaactatcaa tatcagattt tttaaatcta atgaccgtat caatttttat
    10201 aacaggtaac ccgtattgag aaacatattt tctaatcgtt cctactgata cacctagata
    10261 ttcacttgcc tgctgatagt ccatatactc tagagtgact ccagaaataa tgtcttctag
    10321 cataggcttt agcactgtaa ttatctcttt cacttgttgt tcaacttgta tagaaacaat
    10381 gctatcaatg atttgtagag acatatttcc ccctcctttc gtgattgctt gaacaaattt
    10441 tctaagaatt atcttcgtat tttttgctca aaataaaagt attttgttat taaccgatta
    10501 atcaaacaaa aaatgattac cagttcatcg acatacacat aatacctcga taaaaaagag
    10561 gtgtcaatat ctttgtaaaa tttatttaca acaccccttt ttcgaggtat actaatttta
    10621 ggaggtgtgg tcaatatgat aaaacgtcca ccaataaatt atttagaacg aaaaaagata
    10681 ctcggaacaa aaataaaagc tataagaaag agtaaaaaat taacacaacc agcatttggt
    10741 cttatgatta acaacggtca attaattgac aaaaaaacta tctacgaatg ggagaaggga
    10801 acctatttac caataccaga gcgattatcc cgaattgctg atttgggaaa tatgagtata
    10861 gaggagttag tttgtggcaa tgtagaagaa tatattttgg gaattattct ttatagagat
    10921 agcattgtac tagatggtat aacatttcca gataaaaacc tgttccagca tcttcgtcaa
    10981 cagttcccac cagtacactc taatttagat acatggctgg atagatattc caaattagaa
    11041 ccagagatgc aagaatttat tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt
    11101 agtcttttca atatcttaaa aatagaagaa ttattcatca atgctattgt agaagaattt
    11161 gacaataaca ttcttttcct cactagcagt atagaagaac tactagaaag aatggtagat
    11221 gaatggctac ctattcaatt gaaagacatg tcttatcccg aagaagctgt gagagaaatt
    11281 actgataata taaacaagct agaacaaacg atttcatcaa ttgggaaaaa atacactaag
    11341 aaaaaaatga agggaggtga taccatctag tggtaagtac tccttgtaaa ctaagaggag
    11401 atgatttcat ggcaaaaata tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt
    11461 ataaaggaca cattgccacc gatccacgaa ctggtgaaaa aataaatact acgagaagag
    11521 gttttagtag caaacaagaa gctcaaaaag attatgacga gtacagacat caaatattat
    11581 acggtgttaa aaagaaagca cctgatatga catttgaaga cctgtataat gaatggataa
    11641 ctcatcagcg taccagtgtt aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata
    11701 atcaaatact tccagcattt ggtaagctta aactatcgaa tatctctgtc ccatattgtc
    11761 aaaaagtggt agatgaatgg cacagtaaat atgaaagtta cgattatatg agaaaacaga
    11821 ctgcacaaat tttacgctac ggggttgcaa tgcaatatat tgacaataat cctatggaaa
    11881 aaacacttct tccacgaaaa aaagagtatg agaaaaatcg taaattttac tcaaaagagg
    11941 agcttaataa tttactggat gcttttaaag attttggtaa catgaaacaa tacgccttct
    12001 tcagattatt ggcatacaca ggtatgagaa agagtgaagt actcgcttta cagtggaaag
    12061 acatagacac gtttaataaa gaactacatg tcaataaaac tcttgctgta gatgaatttg
    12121 gtaaagttat aattcaatct ccgaaaacaa gagcttctag acgagttatc tctttagata
    12181 ctgagactct ttctatcctc aacaactgga aattacagca aaaagaagag tatttaaaac
    12241 ttggatacaa tactagctct aaagagcaac atgtttttac aacagttaaa aacactctat
    12301 acattccaaa cactgttaat gattggttaa gatatatttt gaaaaaatat aacttgcctc
    12361 gtattacacc tcatgggttt agacatacac atgctagttt attattagaa gcaggagaat
    12421 ctgtcaaggt agttcaacaa cgattagggc atgaaaatag taaagtgaca ttagacatct
    12481 atgcacatat tacaaataat gcacctaaga aaacaggtca agactttgct gatatgatgg
    12541 ctcatcagta aaagagctgg tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa
    12601 agctagaacc cttatat
//
LOCUS       DAZKOB010000078        12587 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000078 DAZKOB010000000
VERSION     DAZKOB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.078"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..861)
                     /locus_tag="ACG8M6_002087"
     CDS             complement(124..861)
                     /locus_tag="ACG8M6_002087"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0457739.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(928..1497)
                     /locus_tag="ACG8M6_002088"
     CDS             complement(928..1497)
                     /locus_tag="ACG8M6_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0457740.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1625..2497
                     /locus_tag="ACG8M6_002089"
     CDS             1625..2497
                     /locus_tag="ACG8M6_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0457741.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            2839..3711
                     /locus_tag="ACG8M6_002090"
     CDS             2839..3711
                     /locus_tag="ACG8M6_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0457742.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            3708..5666
                     /gene="nagE"
                     /locus_tag="ACG8M6_002091"
     CDS             3708..5666
                     /gene="nagE"
                     /locus_tag="ACG8M6_002091"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0457743.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(5998..6849)
                     /locus_tag="ACG8M6_002092"
     CDS             complement(5998..6849)
                     /locus_tag="ACG8M6_002092"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0457744.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            6972..7439
                     /locus_tag="ACG8M6_002093"
     CDS             6972..7439
                     /locus_tag="ACG8M6_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0457745.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(7702..8184)
                     /locus_tag="ACG8M6_002094"
     CDS             complement(7702..8184)
                     /locus_tag="ACG8M6_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0457746.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(8284..9147)
                     /locus_tag="ACG8M6_002095"
     CDS             complement(8284..9147)
                     /locus_tag="ACG8M6_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0457747.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            9745..10620
                     /locus_tag="ACG8M6_002096"
     CDS             9745..10620
                     /locus_tag="ACG8M6_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0457748.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(10765..10917)
                     /locus_tag="ACG8M6_002097"
                     /pseudo
     CDS             complement(10765..10917)
                     /locus_tag="ACG8M6_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(11185..12516)
                     /locus_tag="ACG8M6_002098"
     CDS             complement(11185..12516)
                     /locus_tag="ACG8M6_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0457749.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         3882 a         2525 c         2207 g         3973 t
ORIGIN      
        1 atttgctttt tctgcataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta
       61 aattgatggt cgtttgaata actatttaac aagtaattct ctctggatat tatccgttat
      121 ggattatact tcctgtgtcg tcggacggat aatcatgttg ctgacagaca tccggtctgg
      181 cgtttctatc gcaaatgcta cagcagaagc tatatcttca gatgttaatc cccattcttt
      241 ttgcgcttca tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt
      301 ttgaacagct cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg
      361 taatccttcc atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg
      421 ataaacaaca tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc
      481 catgattgga agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat
      541 attttgccat tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt
      601 attgtacaat acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac
      661 ttgtgcaaaa tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc
      721 ttttattgct tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc
      781 tgctaatagc ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac
      841 tttttctgat aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac
      901 tcactatctc ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt
      961 ggcagggatt cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc
     1021 tgcaacaatg gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat
     1081 ccaagcattt ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc
     1141 ggttagatgg ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa
     1201 acagatgcca cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat
     1261 actaatatgt ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac
     1321 tttgtcacta atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt
     1381 attcagcgct gctaaaggct cttcgttttc cgcttggatc tgatggatca cttcaaagag
     1441 cggatccgtt actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt
     1501 gctccctcct tttatcctta ctttataacg gacggcttat gatgtctaat acttataaaa
     1561 catacttaac tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga
     1621 aaaaatggat attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag
     1681 ccgagcagca aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt
     1741 agaagaagaa cttggtgtga cacttttcac tcgaggctcc cgagagatac agttgacgga
     1801 ttctggtcga tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc
     1861 gaacaattta acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac
     1921 taaagcaatc tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat
     1981 ccagtttcat ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt
     2041 acttgatttt ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact
     2101 gccagcccaa gatcgttggg gacttttgat acatcggaat catcctttag caaagcaaac
     2161 agtcatccat ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt
     2221 ggacagtcaa ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac
     2281 atataactta ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc
     2341 tctagatggg atcatcaata cttatgggac ggaaaaagta ttcatcccac tcgatccgcc
     2401 attacaagct tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg
     2461 taaattttta gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt
     2521 cagcttttta acaacgaata agttaagcaa aagatccaat gattaaataa cataagtttc
     2581 ttaatttgtt ccttcctttt atttctgata attccagtaa attactataa ttataaaaaa
     2641 agaattgaca aaacataact gtctatataa aattgattac gttaacaata attcgtttca
     2701 tgtgactaga tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt
     2761 tttcgtgtct atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc
     2821 ttcaaaaagg gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta
     2881 gatgacggtg gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga
     2941 aatgatttga tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa
     3001 atcaagttgc aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg
     3061 atcattgatc atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct
     3121 ttgaccgacc acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac
     3181 aaacttttaa gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca
     3241 gtagagtatc tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc
     3301 gcaatccata ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa
     3361 gtgacgattg tatctgacat catccattta gtggaatcag aattggaaac agatatccat
     3421 tcagaaccat tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa
     3481 cgaacccatg cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga
     3541 aaatacccag aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac
     3601 cagctcgcga tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga
     3661 agcgcaatcg tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc
     3721 tacaacgaat gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac
     3781 tagtagggat cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac
     3841 aagctggcgg taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac
     3901 tagggatgtc agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag
     3961 ttccagtaaa tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat
     4021 cagttgatcc atcttttgct cagatcagta acgtatttat tggtattatt gccggattga
     4081 ttgcggcagc tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct
     4141 ttagcggcaa gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag
     4201 tgctactgct cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt
     4261 tgagtttagg ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta
     4321 caggattgca tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata
     4381 ttggtaaatt tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat
     4441 ttttcccaat catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac
     4501 gtccagaaaa caaagcaaaa acagcttcct tgatgttagc agcagctttt gcttcattct
     4561 ttactggtgt aacagaacca cttgaattct catttatgtt cgttgcttgg ccattatatg
     4621 tcttgcatgc aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag
     4681 caggatttgc ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg
     4741 cgaatcagcc attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg
     4801 gctttaactt tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc
     4861 tttcagatga cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca
     4921 aaatctacgc tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac
     4981 gccttcgttt gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg
     5041 gcgtacctgg tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag
     5101 tacaattcgt tgcagatgag atggcaaaac tacatggtgg tgctgcaaca cgacctgcac
     5161 aaacaaacac cgtggttaaa acagaaacat ttgccggtgg aacgatcgaa caagatattt
     5221 acgctatcgc aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg
     5281 aaaaaatgat gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa
     5341 tcgctggtac gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg
     5401 ctggtatcga agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat
     5461 tcactttata tgtaaaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg
     5521 atcttgccgc tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg
     5581 gcgacaaagt ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa
     5641 tcggctctgt ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa
     5701 caaacaggag ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt
     5761 ctcctgccaa ctccgcggta ataagtccag ccaaacgtaa catatgaaga gctatttttt
     5821 atttgactct tcttattact tgatgcagaa cagctttttt acaacctctg ataaacggtg
     5881 ttcaaaaacc agtttatcgg tatgaagtca aatttatgca aaaatgtgga aagccatttt
     5941 cacaaaattt ttcttcatac tcaaaactaa tcggcttttt cacaacctct ttatacatta
     6001 ttcctgatcc aatatttctt ttaatgctgc ttcaagagaa gttaatggtt ttcccaatac
     6061 tttttctaaa tcatcagagg aaatagctaa ctgatccttt ttaatatcat gttggatcgc
     6121 taaaaacatg tcagctgctt cttgagggaa tccggcttct tttagatttt cgacaaacgc
     6181 tgcatcactt gcttcaataa tttctaactt tttacctgtc gcacgctcta atgccttggc
     6241 taattcttca tagtcagtta gattgccgga taattctaag atttctggag agtcactttc
     6301 tactagtact tttgctgcag cttctgcata ttcacgtttc aatacccaac ctgtttttcc
     6361 atttcctgca gcataaacga attgaccagt ttttaaagcc tgcccaatga taggtaattc
     6421 attctctaca taccaattat tccgtaaaaa tgtatgggaa atacctgaat ccttaatcat
     6481 tttttcagtg aattggtgat ctggcgctaa tacactgatc gagtgatcgg catctgcaaa
     6541 acttgtataa gcgatataag ataccccagc tgcttgtgct gccttcacta cattcttatg
     6601 ttcggcttga cgatttcctg gaacgccaga gacaaaaagc aatcgatcta ttcctttcaa
     6661 cgcttctttc attgagtcta aatcatcata atcaccaata cgtatattaa atccagcttc
     6721 tttcagtgct tctccttttt cttcactacg tactagtaca tagatttcat tttgtggaac
     6781 catctttttt aagaattcca gcgcataacc accaaaacct ccagttgctc ctgttactaa
     6841 ataatgcata tttgccactc cctattcttt ttatttgtta agatttacag ctgtacattt
     6901 ataaattaca cctttatcat aaagagtgtg gtaagataag tcaagcaaaa aaaatgaaag
     6961 gatgacaact gatgaagtat tctctgcaat ttagtgatgc aatccatgta cttgcctata
     7021 ttgaaatgtt tcaagatacc cgatcacttt ctagtgagat gattgccagt agtatcgaaa
     7081 caaatgcagc aaatgtacgt aaaatcatga gtcagctcaa aaaatccggt ttgatcttta
     7141 caaaaaccgg aaaaccgcag ccttcccttg ccaaaagtcc agatcagatt tctttactag
     7201 atgtctatcg cagcatcgaa ggtaatacac agcttatcca agtcgatcca aaaacaaatc
     7261 caaattgcgt cgttggtgcg aatatccaag aagctttaga agaaagctac gctcggctgc
     7321 agaaaaaggc agaagaagaa atggctaaga tcacacttga tagtctgata cataaaatag
     7381 ccgttttgga aaaagaaaaa agaccagaaa acacaggcct cgtagagaga tttttataat
     7441 tctctctact gtgcctgcat ttttctgatc ggaatcgtct acataaattc ttttattctc
     7501 cgggggacaa aagccttgtc atgccccttt ttcctctcta atgctcaggt caaagcgctg
     7561 aagtcatgac gtcttgataa acggtgttca agtagctgac cctcggcatt agttctgaaa
     7621 aatgcaaaat atggaaagct atttcccatt tttcctctct aatgctcagg tcaaagcgct
     7681 actttcacga cctctatatt atcatggttt attgactacc ggtatccaga tttctcccat
     7741 atatttcccg ttggtaactc ccatttcgac tacagcattt ggacccccaa cataggcgta
     7801 atcttctact tctcttaaaa catgcccaaa ggccgtattc gtcaaatcat ttcttaattc
     7861 ttcaggcgat tctgctaaag cgattacaac aagatacgtt ccttttggga atgggatgat
     7921 tcgggtagtg tttggaacat cctgactcgt ttgaacaccg acatagtaca tcattttccc
     7981 gttatagggt tcgttgactg taaagaaata atgattttca gcagcttgtt tcaatgcatc
     8041 tattgttcca ttaccgataa ctttttgctc aaatgcttgt ttctctgcag ccaaaccgac
     8101 aaaatccgta ttgctactct tcaattccac accaataccc gtcaccgtaa aatctggttt
     8161 ttcttcgatc ctatactttg ccatttctcc caccctttct attatattaa gttcatctta
     8221 cataaaatta aagggaaatg acacgattac gctctttttt ctagggatat ttttattttt
     8281 taactatttt tttgctttca ctgcaatcca aatctcactg taaacattac tgaaatcctc
     8341 tccccatttt gtaaaagaga tctcgggagc ttcgactaac tcataatctg aagaaggaag
     8401 ccattctgca tagattttcc cccaagtatc ttgtaaagtt tggggaaatg gccctttatt
     8461 aggaaaaatg gcccaagtgt gttcttctat agcaagcatt tctaaatcag cgtacggatt
     8521 ttcttttgtc gtagcaaagc caatcatgtg catcaaactg cccttttctt ccaagcgggc
     8581 tccatcaaat ccataagaaa cgttgattac ttgatttgga tataaatctc ctaacttatg
     8641 catctcttcc ctttgagtct ccgtgatcgt ctgcgctaac ttttgtattt cttgattgac
     8701 tccttcaaat ttgacaggaa cttttttcgc cacacctgcc acataaaact gttctttctt
     8761 ttcgatacga aattccatcg attttcctcc tctgacatca ataaaaaatg tcaagcgtgg
     8821 aaaagatttt tgtatgccgt atctgctgat ttctgaaggc agatatcctg accaatcacg
     8881 aaaagctctt gaaaaacctt cgactgtttc atatccatat ctaaacgccg tttctgtcac
     8941 tcttgatcca tctaggagat cgcgattagc caaagccaat ctacggtttt tgatatagtc
     9001 cattagcgac atccctgcaa taaacgtaaa gatgcgtttt aattgatatt cagagattcc
     9061 tgtattttcg gctattattt tataggaaag tccttctgtc aaatgttctt cgatgtaatc
     9121 gatcaattgg ttcagatctt taagcatatt tatcttcctc cttgcttgcc tttttatcat
     9181 attatcaaag aaagtcaatt acttgatgaa tcaggaacca atttagtcag atgtagaaaa
     9241 tgttgtatgg atctaaagaa aattctcaag cagcgtttcg taacggataa ttggtattat
     9301 ctgcctatct atgttttacc tttagaaaaa taaattttgg ggaagacaca ttctaagtaa
     9361 acaaagcggt aaaaatatct tttattccct tagttaatcc gcttactacc tactgtacaa
     9421 agcaaattgt tgttcaaact gctgatcaga acgctgcttg gatttttctt tgcgatacat
     9481 catacctctt ttatcaattg gaacaattat ttatttctaa caaaatcact tcatttttgt
     9541 cagataaata aaaaacaaaa aaagttccat ctttaaaaca cgctattctt ttactttttc
     9601 tcacattctc taagaaaaga aaaggagaat gaatttttga caattcgagc aaaagtgatt
     9661 atcaaatatg taaaatcagc tacttttttc ttaaacgttt ctttatacta aatataaata
     9721 agtgtttcat gaaaggagaa ctagatgaat aattggcttg agtattttcc tgaaaacgtt
     9781 ttggaacgcg gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc
     9841 aagtacttgt ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat
     9901 gaaaagacca acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac
     9961 atggctgctg tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt
    10021 tccgaactct cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca
    10081 gagattctta tagaacatcc gcaatttatc gaacgcttca aagttaagat gccatttcat
    10141 gcaataaatt attcagataa attaacaact atcatccata aatacgatca tattatcaag
    10201 aaaaacaaaa acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa
    10261 gcagttgaat cgctcattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa
    10321 gtgattgcta ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg
    10381 atagaggact gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa
    10441 aggcaaatgt tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa
    10501 cgctactcca taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag
    10561 aaaaaaatag ataaaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag
    10621 caagtaaacg ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa
    10681 acacgaatca tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt
    10741 tgactattag gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctgggta
    10801 gattttccgg cattgttgat agattttatg atacttttct acattttgta agagaggttc
    10861 cactgcatct gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccactc
    10921 taatctttaa agataaatat taaaataacg attaataata ttaacagtct ttatttttca
    10981 ccaccaaaaa cctatctttg catatactaa atagacgtat atacacagat aggtttgtct
    11041 tgtgaaaaat aatgattatc cttaccaaaa aatgtttgtt tttctacatg ttcgtactta
    11101 tcaaaaaata tgatccttat tgaaattact ggctatttat attccaacga cttcttgtgt
    11161 taatttttgt tatctcatag taaattatgt ttcattttca cttaatttag ttgtcacgtg
    11221 ttcgtcaatt cgtaaaaacg gtaaatatat aaacacactt gcggctataa ttaatatttg
    11281 taaaacagct gctcgccagt ctccggctgt tgctaaaaat cccgatataa ctggtggcgt
    11341 agtccaagga atttgcacta ctacatgatt aattaatccc acagcagttg caaaatatgc
    11401 tactgtcaaa gaaaaaatcg gtgcaagcac aaagggaata atcagactga tattaaatac
    11461 aataggcaat ccaaaaataa taggttcgct tatattgaat atagcgggag ttaccgataa
    11521 tttagcaaag tccttataat cttttctacg actgaagata aatattgcaa tcaataaagc
    11581 aatcgtattt cccgatccac ccattaccgc aaaggtatct ttaaaggcca tattgataat
    11641 atgtggaatt tgctctttgt ttgcataagc tgccatattt tcttgcatat tctgaatcaa
    11701 aaacggatct aacaatgaac ctgatattac tgcttgatgg ataccaaatc caaaaaatag
    11761 attagcaata gatgtaatta acaaaaatcc tggtaaacca gttgtaattt tacttaatgg
    11821 ctctgtaact atcgtggtaa ttagcttatt tatatccata ttaaacaatt gaatgattat
    11881 aaatgacaat aaagcaaacg aggtaatcgt aataatgatt gggattaata cattaaaaga
    11941 ctttaaaaca gcgggaggaa tatttccgga tagctgaatc tgtagtttct tattattaga
    12001 aagtttaata aataattctg tagccgctaa tccaacaaat atacctacaa acattcctga
    12061 agctcctgta taggatatag gtatcacacc cgtcacttca acagctttat ccgcattttc
    12121 aggagtaaac atcatctgca taggtgttaa tacaacaaat gtcgaaatag caactaatac
    12181 cgctgcaata tgatttgtat aatttctgct tgcacacata tgataagata ctgctgctac
    12241 aactaataat gtagttatgc ttaatgtacc attggcaatt gaagtaccaa tttgttgtaa
    12301 cgtggtcaat gtactagtat cgacaatttc ccccatgaat cctgttggtt ctaagatcac
    12361 tgaattaata agtgtcacaa atcctgctaa catcataaat ggcataaatg tagcaaacgc
    12421 atctcgcaaa gttcttaaat ggatctgatt ccccaactta gtcgcaacta taccaaattt
    12481 ttcaaataca atgtccttcc tgctactttt gcccatattt tttctccttt atgcttattg
    12541 taatcgatta ctttttccac ccaaataata catcacttat caaaaaa
//
LOCUS       DAZKOB010000079        12519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000079 DAZKOB010000000
VERSION     DAZKOB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.079"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1105)
                     /locus_tag="ACG8M6_002099"
     CDS             complement(<1..1105)
                     /locus_tag="ACG8M6_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0457750.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVK"
     gene            complement(1221..1787)
                     /locus_tag="ACG8M6_002100"
     CDS             complement(1221..1787)
                     /locus_tag="ACG8M6_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFQ0457751.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(1923..2576)
                     /locus_tag="ACG8M6_002101"
     CDS             complement(1923..2576)
                     /locus_tag="ACG8M6_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457752.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(2626..3054)
                     /locus_tag="ACG8M6_002102"
     CDS             complement(2626..3054)
                     /locus_tag="ACG8M6_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0457753.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(3059..3433)
                     /locus_tag="ACG8M6_002103"
     CDS             complement(3059..3433)
                     /locus_tag="ACG8M6_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0457754.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            3737..3922
                     /locus_tag="ACG8M6_002104"
     CDS             3737..3922
                     /locus_tag="ACG8M6_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457755.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            3935..4183
                     /locus_tag="ACG8M6_002105"
     CDS             3935..4183
                     /locus_tag="ACG8M6_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457756.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            4214..4384
                     /locus_tag="ACG8M6_002106"
     CDS             4214..4384
                     /locus_tag="ACG8M6_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457757.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(4486..4953)
                     /locus_tag="ACG8M6_002107"
     CDS             complement(4486..4953)
                     /locus_tag="ACG8M6_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457758.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            5112..5429
                     /locus_tag="ACG8M6_002108"
     CDS             5112..5429
                     /locus_tag="ACG8M6_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457759.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            5362..5841
                     /locus_tag="ACG8M6_002109"
     CDS             5362..5841
                     /locus_tag="ACG8M6_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFQ0457760.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            5858..6034
                     /locus_tag="ACG8M6_002110"
     CDS             5858..6034
                     /locus_tag="ACG8M6_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457761.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            6291..6722
                     /locus_tag="ACG8M6_002111"
     CDS             6291..6722
                     /locus_tag="ACG8M6_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457762.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            6728..7045
                     /locus_tag="ACG8M6_002112"
     CDS             6728..7045
                     /locus_tag="ACG8M6_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0457763.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            7164..7850
                     /locus_tag="ACG8M6_002113"
     CDS             7164..7850
                     /locus_tag="ACG8M6_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0457764.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            7825..9174
                     /locus_tag="ACG8M6_002114"
     CDS             7825..9174
                     /locus_tag="ACG8M6_002114"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0457765.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            9165..9638
                     /locus_tag="ACG8M6_002115"
     CDS             9165..9638
                     /locus_tag="ACG8M6_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0457766.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            9640..10140
                     /locus_tag="ACG8M6_002116"
     CDS             9640..10140
                     /locus_tag="ACG8M6_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFQ0457767.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            10155..12467
                     /locus_tag="ACG8M6_002117"
     CDS             10155..12467
                     /locus_tag="ACG8M6_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFQ0457768.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
BASE COUNT         4152 a         2141 c         2529 g         3697 t
ORIGIN      
        1 cttttactcc ttttgtcaca tgcaaataaa ttttttcagt gatatcacta ttttcgtgac
       61 caacgcgatc ctgaatggca tacagaggag ttcctagttc cgctaatttt gaaatatggg
      121 tatgtctaaa aatatgtgaa ctaagttttt tatcaatctt catgtcagct ttatgacttc
      181 tcaaataggt gttaattgct gttagttgga aaggagttcc tttcgttgtt tgaaatagaa
      241 attgaccatt tggatttaat tctagaagct cattgtaaat agctatcgct ttttttggta
      301 aatcaatttc tcgcattccg gcagctgttt ttgtagaatc agattttttc atatcagcta
      361 ttgaacgttc tctatacatc atcgtcccat ttataactac tgatgcatta ttattggtaa
      421 tgtgtacatc atctttactt aatgcgattg cttcaccaga cctcatccca gtcaaatata
      481 gccactggaa aaggagagaa tatcttttat tgtgtgaagt tgtgaaatct actagtctat
      541 tatattcatc atcttctaaa aatttatctt taatttttat cgtttttgac tctcttttat
      601 aatcaataac tacttcatcg ataggatttt tttctacata accttttttc atagcatatg
      661 aaaaaagaag attcaatttg gatttaatta cactgacgta cttatttgac aagtcatctt
      721 tatatatcat gtcttcaaaa atattattca aatctattgt attaatacca gaaacgatgt
      781 aagtctcagg tatcttcttc tttatagtgt ttaatatatt attcgtagga taatatgtag
      841 attccttaac ttgtcttttg tatataacta accattcttc aacaagctca tgaaatgtta
      901 gatcaggctt atgaagtgtt ttttcgttta attttatatc aattttttta tttaactcta
      961 gcaatgccac tttctgagtt tctcgagatt tatttttata agtaatgctc actttttttc
     1021 tttttctagt ttttggatca atatatcgct cattatattt atatactttt tttccgtttt
     1081 tatcagtttt agtttcgatc cacattgttt tcatctccta tttgttgcta taataggaat
     1141 agataagtaa gccgattata gcaggtttat ttttcatcac gtccacaaac tttggcgagg
     1201 gaggggcgtg tttttgttag ttattgtggt aggttatcta aagcgtattg tgcttgctca
     1261 tttgtaaaac cttcaaatat aagttgatcg tacaattgcg aatctgacat tgatgcccaa
     1321 tcatcgtaac tttccgcttt agccaaagct tgttcgttgt aatcgacatc aacattagcc
     1381 agcgcataat cgattgcatc acttggatat tcttcaaact ctagttgttc acgcaaacct
     1441 tgttcagaca taccagccca acctagatag ctttcagctt tgctcaatgc atttctgtat
     1501 tcacgaggaa cactttcttc ttgagtaact tcttctatag tagtttcagt agtttcagtt
     1561 tcttcttcaa cgactgtaga ttctgctacc gattctgcag aagacacttc tgtcacgctt
     1621 tctttagttg tttcagcgac ggtattttta gcatcatcac tgcctccttg cgaacctata
     1681 gcaataaaca ccacaacaac cgctaacaac caaaaccata cccgtttata aaacggtttc
     1741 ttaactttat acattttccc atcttgaccc ataacttttt ttgccattta aatatccctc
     1801 gcttcttgtt ataatatatt tgtgatctca gaaatgaggt atgagtccgt gttgcagcac
     1861 gggctttttt ctttataact tttttagaga ttataggcaa aatagtaggg cataaaaatg
     1921 tattattgaa ttccgtattt agaaaatcct aattgaactt caccggaagt cttttgctgt
     1981 gttgtacgca atgcttcttc ttcagacatt ccattctgta ctttccatgc aacaggcgac
     2041 atcccgtatt tgttaacaaa atcagtaagt gataaagtgt cagcgtcttg ctgagcgctt
     2101 gtttgttggt cttctggatt ctgttgagat gctgcttgtt gttctttctg atcttgactg
     2161 ataatattgc cagcatcatc tgtagtcaat ccattttcat aaagggccac gccgaaagct
     2221 tcccactctt tgttggacca atttgcacga tcagctggag ttgactgtaa agtgcgttgt
     2281 ttcatctgtt catatgtttc ttcttgaggt gcggtttgga ttgtactctg actggagctt
     2341 ataactgttg ggctaggttc cgccgtagct tggttggagc ttgtaactgt tgaactagaa
     2401 tctgttttag atgtagattt gctagtagat gaactggttt cagttgtttc ttttgtttta
     2461 cttatttttg tttcttggtt agaagtggta tctgttgatt cagctttttt attatttgaa
     2521 caagctgaaa gtagcagagc agtacttaac aacaacataa cgctaacttt tttcatttta
     2581 taattcctct ttctcgttgt aatatgtgtg ctaacacggg cttttttata taagaaaacg
     2641 ataagcgctt tctggaagcc cgtaaagatt ctttaattcc tcgatttttt taggatattg
     2701 atcattatct tctttataaa gagaaacaat gagattagca gcaaagcaat tagcttcgct
     2761 ttcagatttg cttctagatg ttcttgttga tacataatag ctggataagc cacgatgaaa
     2821 aatagcgtga cctaattcgt gagcgcaaat gtagaatctt tcctcagagt ctcgcagttc
     2881 atcatttaaa aagattatcg cacgatctct aatttcttga aactgcccct tgggattctc
     2941 gataaaagga acgtattgaa ttttaatgcc catcttttca caaatataaa aaggattagc
     3001 ggactggtat ttccgcttca actcctcgac taaattaatc gtatccatct ccataagctc
     3061 acatcttttt gcctttttct ttgtcttctt tcacaatatc ccagaaagtc gctatcagga
     3121 tatcttttac gcgctgtatt tgttcgggtg tcaatgtttc cccaccataa gacatattaa
     3181 catttgagtc tagtagttta tcaagttcaa ccacttcctc ttttgtagcc cacttgggaa
     3241 cattattatt tcccaataaa taatcagttg tgacaccaaa ataatcagca actttcttta
     3301 agttctcaga ttttggcgag gctttatccc atcttcttat ttgtccatta gagatgccca
     3361 cctgtctttc tacttctgct atagtcacat gcttttcgtc tgctaattct ttaatcttag
     3421 taactaaact cattattatc aacctttcaa agctgaaaag aaaataaata gcttaaaagt
     3481 tatatttttg gttgacaatt agcttttaag ctagtatatt taattcgtaa gctaaattgt
     3541 tagctaaata agagcaacaa aaaactctac taatttaaaa cattctctcg gtcgccaaac
     3601 ttagaaatgt tattttagag gctttttata agtcttattt aactatgtat tcataatagc
     3661 ttaaaagcta ataggtgtca acgatttagc taatttttta gcttacaaat tatttgttta
     3721 gaaaggagct atttttatgt ctgagaattt agacttaaaa attcgagcgg agatgagaaa
     3781 aagaagaatg actttcaaag aactagctgc gcttatcggt atttcaggag cttatttatc
     3841 agatattcta aacggcaatc gtgatggaaa gaaagcacaa cagcatatcg aaacagtgaa
     3901 aaaaatattg gacatccgat agggggggta ggcgatgaca aaactaaaaa aacaagattt
     3961 tgtaaaaaaa tataattatt ctccatctac ttatcaacgt cgaatgtcgg aactaaaaaa
     4021 tacagcaatt ttctcagcgg cgtatgaacg ggtcacagga caagaagttt ggatcaatac
     4081 agaattatac gataaatttt tgtctttcaa atcctataac aggttacgca caagaaaggt
     4141 aacacctaaa gaatttatcg agaagcattt agttgattta taaaaaacag aacattttga
     4201 gaggtgaagg ttaatgggta aattcaacag agcattagta ttcagcgcac cgctaatcgt
     4261 ctatgcttta gggctttggg gaagtagaca agcgttgata ggaacgattg tgtacatggt
     4321 ttggattttt atagggctgg atgaagctga gtacaaaaca aaaaagccag tcgggaggga
     4381 ctgactaatg agtaaggacg atattgtatt tctgttaatt atgttttgtg ggattggatc
     4441 aataataatt aatttgtatt tgattattaa agaaaaattt ttcaattaaa atgagtcttt
     4501 tagctttaac ttaaattttt tttgataatt agctgttctt atgaccatta caagtttttc
     4561 accaggtaat atatctttca tatctaaaac ttctgaaaaa gcgactgagt agccaggaga
     4621 gattgcttgt gccaacggta taggagataa aaggctactt ttatgaatgc ttcccatatc
     4681 aaaaccattg gcgtgataat gtgaacaatt aattttctta ccgttgtaaa gatacgtaca
     4741 ttcgataatt gagtttggca tttttgatgg gtttgcaatt acgccagtaa gaatcattcg
     4801 attacttcta tcatgcttaa ctaaaaaata ggagagcgta atttttcttt tatctttaaa
     4861 taagttatag agggataaaa aaaatcctga aatagaaatg attaaagtaa tagcgacttg
     4921 gtaatcgctc aaaattttta tcaagtaatt caccaccttt ttactcaaat tatatcaaaa
     4981 aggagagaag aaataatgca agaattagta attttgaaaa ataaaaaaag acacgaacgc
     5041 cggcaagcat gatcgtgtca gggaaataaa ctatctttct atattttacc acagaaagga
     5101 atgaaccgta aatgctaatt gcaacggata cactagaccg catcttttta aaagacgaat
     5161 acaaactgcg caatatagat gcgtcaggaa ttttagtatt tgatctttat gacaatggaa
     5221 aaattggtat ctatcaagca agtgatattg aagaaacaaa ccttgctttc gaacaaattg
     5281 atgattctgt ggaattggat ttagatgagg caatcctagc ttttgaacaa attgcaaaat
     5341 tattaaagga ggcacaaaag aatggcaact ctttaccaac tcagcgagtc atatatcaaa
     5401 gtcctggaac tggcagaaca attggatgaa gaaattcttc gcgatactct cgattcgatt
     5461 aatgaagcga tcgagtataa ggcagaaaac ttagcaaaaa tagttaaaga agtagaaggg
     5521 aaagctgagt taatcgatag tgaaattaaa cgtctgcagg aacgtaagac atcgcttttg
     5581 aacaatgcta agagtatcaa gcactattta caagaggaaa tggaaaagac tggtaaaacg
     5641 aagattaaag gtgaattatt caacattgga attcaaaata atccagtatc ggtcaatgta
     5701 atcaatgaga acttaattcc aaaaggattc tttacccctg tgcctcccaa attggataaa
     5761 aagcaattga aggaggaact gaagcacgga gatattcctg gtgctgaact cgtccaaaca
     5821 aaaggtttga ggataagatg atgaaggagg agaaataatg gaacacgaaa acgtaaaaga
     5881 cgctttgaaa gctgctatcg aaatcgcgga agctaaagga ataaaggttg acggcaaacc
     5941 agcaactgtc catgacattc aaaatttgac gaaagaacat ctttatttta tcgccgactt
     6001 gcttggtttg tcggaattgt atttagacaa ataaccccac acggaaggag tggctagggt
     6061 ggttgtatta caagtaatag tctgcgtaac attcatttgg tcgtgcatac cggtttgtat
     6121 atatatcatg aagaagcttc gccctgagtc ttacaaatta tacgcaattt acagcataac
     6181 agtagctctt gcatttacat tgtttgcaat ttctaggctt ccgttttaat cgtgggaatg
     6241 aatttttaat gaactaaatt atctataaag caaaaaaagg agaaacaaca ttgaaaaaca
     6301 aactagcaaa aacaacagca atcatcggac tagcactagg tagcggcgtt atcggctacg
     6361 cagcaagcaa tgcgtttcaa gatttggaca cgatcaaagc aaatttcaac acagtcctac
     6421 aatacggtca aacgaaatcg caacgtgtgt cagaacttga atcacagcta tccaacaaca
     6481 ctcgcacaca ggagcagctg aaagccgaaa ttgagcaaat caaatcggac aagcagaagg
     6541 aaattgaagc caagcaacga gaagtcgaac aaaagcaaca ggagatcgct acaaagcaac
     6601 aggaagccga tagcttgcgc caacaactat ccacagtgca aaacgacaag gagcagttag
     6661 aacagcgcgt gagcgagtta cggcagtata cggatcaaaa ggtaggggag ttggggaaat
     6721 gaaagagttg actatttatg acccaaatat ttcatgggct aaacacacaa ttaaagtttc
     6781 ttttatgatt tggggttaca aaggatatgt tacttacaaa gtgggcggta acactaaggg
     6841 tttatcatta atagctattg attcagatga tctatatgat gctaatttcg aggataaccc
     6901 agttaatttc cgtgaccttg atgaagattg gttctctatg gaacttacta acgataaagg
     6961 cgacagtaca cttgtagaag atgaattcga cagactaggt gattatattg tcggtgttga
     7021 aattattgcg cacgaaccag agtaggagag tgaatacaaa atgattaaaa gaatcactat
     7081 atcagttgaa gaagatagtg gatcaacaat gcagcatacc gacaatcgaa aaactttttt
     7141 aaccaataga aaggagaatt ttgatgcaga ttaagaaggc gacggatatc gaacaaacca
     7201 agggaacata tttgatctac ggtgctccag gaaaaggtaa aacttcaacg gctaaatatt
     7261 ttccagggaa aacactgatc cttgatattg atcggacttc aaaagtcctt agaggtgaga
     7321 aaaatgtcga tatcgtatat atcgacaacg aagatacctg gaacgactgg ggcaacacgt
     7381 tggctgattt aactaccaat tatgtaggtg tttatgacaa cgtggttgtc gataatgtca
     7441 gcgaattgga acggtgtatt ctgtccagtt taggcgcaga aggaaaaaac aacggcgttc
     7501 cttcgcaagg cgattatcaa tatatgcaat ttagaatggt gaacagccta cgctatatga
     7561 aaaatttaga cagcaacctt atctggaccg catgggaaga aatcgatttg tggacggatt
     7621 cggatggatc ttcttacaac atcgctttgc cgcagatcaa tcgaaagatt cgaaacaata
     7681 ttttgggatt atgtgacgtt gttggtcgat tgatggtaaa ggaagacgat gaacgcggat
     7741 ttattttaca agccacagat tcaacctatg ccaaaaatca attggataat agatctggct
     7801 gtaagcaaag tgagctgatt ttagatggaa ttgtacgact accagaatga tttagtcgat
     7861 cgtgcaagac aagcctatgt tgatggttac aaggcaccat gtatcgtatc accgtgcggt
     7921 tctggaaaat cagtcatggt tgcagagatc gctaaacgaa ccacacgaaa aggcaacaga
     7981 gtgttgttct tggtccatcg tcgtgaattg attgatcaga tcaaagatac gtttcaaaag
     8041 atgggcgtag ataatcagct tgtaaatttc ggcatggttc aaacagtcgt acgacatctt
     8101 aaaaccatca agaaaccaca actgatcatc actgacgaaa accatcacgg gttagcagcg
     8161 tcctacagaa aaatctatga gtattattcg gacgttccgc gattaggttt taccgcgaca
     8221 cccatccggt taaacggtag tgggttgggg gatgtgaacg acttgctaat tgaaggagta
     8281 tcggctaaat ggttgattga gaatcataga ttagctcctt atgaatatta tgcacctaaa
     8341 ttgatcgata cagccgaatt aaagaaagct tcgactggag attttacaaa gaaatcaatg
     8401 gataaggcag tgaagaatac tatttatggc gacgtgttga aacattataa aacgttagca
     8461 gaaggtgaac aggcaatagc atactgtcac agtatcgatg caagtaaaca cactgctgaa
     8521 atattcaacg atgctggtta taaagcaaca catatcgatg caaaaactcc aaaagatgaa
     8581 cgtgcagata ttatcgaagc gttcagaaat catgaaatta aaatcctatg caatgttgat
     8641 ttgatcggag aagggtttga tgttcctgat tgttcgactg taattatgtt gagacctact
     8701 caatcgttat cattgtacat tcagcaatcg atgaggggga tgcgttaccg accagataaa
     8761 acgtcaatca tcattgacca tgtgggtaat gtgaaccgtt ttggattgcc tgatatggat
     8821 cgtgaatgga gtttagatac aaaaaagaaa acaaaaactg atagtgattt atctatcgtt
     8881 caatgcgcgt tttgttttgg tgcttacgaa cggccaaaag gcgataatat ttgtccgtat
     8941 tgcggagagt tgcagccgat tgaagaacga aagtcagagt tagaaatcga tgaatcggct
     9001 gaattaatga aagtcggaga aacgaaaatt actttgaatt ttgagaacaa taaatattac
     9061 aacatgacgg aagatgaagc agaaagtata gaggacttat atgccatagc aagagctaaa
     9121 ggatttaaac ctggttgggc ttatatggct gctaagagga aggggtggtt gtaggaatgg
     9181 gcggtatttt tggtaaaaaa gcagaagatg ttcaaggttc aataataaat ggcgtaacaa
     9241 taataggtga tactggaaaa agagatagtt caggcaatca aattgtaatc gctagaaata
     9301 aaaataacga agtttttgaa tcttctctat ctaatataaa aagaggtgtt gttaacggga
     9361 caggcgaaga gagtggctac gttaatttaa aaaaagctaa tgaaaaatac catgttgaag
     9421 gaacttacgt taaggctttg tctgtcggtg gaacaaaagg cgttaattgg gataaacgaa
     9481 gaaataaatg gaaagcacga ttatatttca aaggagaata tgtcttacaa aaggatttca
     9541 acaccaaaga agaagcaatc caagcacgac ttgcagcaga agaaaaatat ttcaaaccta
     9601 tcttagaaaa atatgaaaat aaaaaggaga atgattaaca tgacaggatt taacttagac
     9661 ttttcaaaag cgcaacaagg gaacgaaatc aaagacggta cgtacgaagt ggtcgtaaac
     9721 aaagctgtag aaaatgcaac taaatcaggc gcagaattta tcgatatcga tttgatcgtt
     9781 cgtaacgatg tggatcaacc gttccaaaat aaacacattt tcgcaaaaat ctggaaagcc
     9841 aaagctaccg gcaaatataa cgaaggtatg atcatggcaa ttgcacaagc gctacagttg
     9901 gaagacggaa aatcatataa cgggtttgat gaattgctgg cggactttgt tttgaaaacc
     9961 gcctcagtac gcgtgaaaac tgaagagtca aatggatata aaaacgtcaa tgtgaagtct
    10021 tgggataaaa cgaacacacg gggcgtaatg aatcaccaat tcaaaaatgg tgatgaacca
    10081 tccttcggac cggaacgttc aagagcgaca acggttagaa atgacaatct tccattttaa
    10141 ttgaggtgta atcaatgtat gaaaaaattc caaaagagct taaaaatctc aaacaatggt
    10201 gtgtttataa gctagtttgg gatgaaaagc ggcagaagca cacgaagatc ccttataacg
    10261 ccaacaacgg gcataaagcc aaatccaatg acgagagtac ctggtctgat tttcagaccg
    10321 ctctcgctgc tattgatcac tacggaatga gtggactggg gttctttttc aaaccacctt
    10381 atttcggcat tgatatcgat aatgctgaag gtgaagttga gcgatttaaa tcaggtgata
    10441 tagaagaaaa tattatctat gaatttatcg aaagcatgag atcctatgct gagtattctc
    10501 aatcaggtac tggcatccac attattgctc gcggagaatt gcccggcgga agacgcagga
    10561 aaggtgacgt agaaatgtac cagaacggcc gattcttcgt tatgactgga aatgccgcat
    10621 caaaatattt agaaataact gagccgaatc caaaggatat caaacgccta tatgatcgct
    10681 acgttggaga tgaaaaaatc atccaattca aagaagaaaa tccgctgatg aataccgttg
    10741 atttaccggt tgaagaaatt atcaaacgtg ctgaatcgtc ttcccaaggg gcaagattca
    10801 aagtctttat gaacggcggt tgggaagctt tttattcctc gcaatccgaa gcggatatgg
    10861 cgttcgccaa cgacttggct ttttggaccg gtagagactt tgaaaaaatg gatgagattt
    10921 tcagagcgtc tgcaatgatc cgtagcaaat atgaccaaaa aaggggatcg gcaacttacg
    10981 gagaatctct actaaataag gcaattgctg atacaaattc cgtctacaac cccaaacgga
    11041 aatctgattt taagattttt atcaaagacc aggaacagcc aaaagaggaa aaatactatt
    11101 cgtacgacga caccggcaac gcggatcggt ttactgatat ctacggaacg ttagtcaaat
    11161 attcgtacat tgataaatct tggtattact acgatgggaa agtttggcta caggacaata
    11221 ccggcgaagt tcggaaaatg atcgatacga cagtagacat catgggaaaa gaaccgttaa
    11281 cgatccctga aggcgcagac gatgaaacca aagaagcttt gatgaaagcg aaagaaaaac
    11341 acgtcaagcg ttctcgcagc aatgccggaa aaaacgctat gatggacgaa ttgaaacatc
    11401 gactatccgt attgccggaa gagttcgaca aagataaaac gctgttcaac acacaaagcg
    11461 gctatctgtc tttgcatgac ggtcttctac acgagcatga gatagacaaa atgtttacac
    11521 gtgtttcaaa tgtcgagtac acagaatcgg tcgattgtcc tatgtgggaa gaatttatta
    11581 atcagatttt tgacaatgat caagaattaa tcagatatat ccagaaatgt gtcggatatt
    11641 ccctgaccgg atctaccaga gagcaatgta tgttcatcct ctacggtcat gggtcaaatg
    11701 gaaagtcggt atttttggaa attatttctg aattgatggg aaactatgcg atgactatgc
    11761 aggcacaaac gatcatggtc aagcaaagcc aatcatcagc aaacagtgat attgcacgat
    11821 tgaaaggtgc gcggttggtc acttcgtcag aaccgaatga aggcgtccga ctggacgaag
    11881 gtctggtcaa acaattgact ggtggtgata aagtcacggc tcggcacctt tatggaaaag
    11941 aattcgagtt cgaacctgaa ttcaagttgt ggctggcaac gaaccacaag cccatcatcc
    12001 gtgggaccga tgacggcatc tggcgacggt taaatttaat tccattcact gtccaaatcc
    12061 cggatcacaa aaaggataaa aatctgaaat ttaaattgca gacggagttg caagggatcc
    12121 ttaaatgggc aatcgatggt tgcctgttgt ggcagagaga gggcttggag aaaccaaagt
    12181 cggttgttgc agcgagtcag gattatcgaa acgaaatgga tcaaataggt acatttatcg
    12241 aaacgtgttg cgaaacaggt ccaggactaa aaatatcagg cggagaatta tacaaagttt
    12301 atcgtgaatg ggcatcagat aatggcgagc atactttcac taacacgaag tttggcagag
    12361 agatttcaaa gaaatatagc aaagaaaaag ctggtggatt tatggtttat aaaggaatca
    12421 cattaaaacc aagaaaatac gataacgtta gagaactttt taaataaatc tggagggttc
    12481 tggagggttg agaacgaaac cctccaaccc ttagaaacg
//
LOCUS       DAZKOB010000080        12491 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000080 DAZKOB010000000
VERSION     DAZKOB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.080"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG8M6_002118"
     CDS             <1..375
                     /locus_tag="ACG8M6_002118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457769.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG8M6_002119"
     CDS             complement(324..893)
                     /locus_tag="ACG8M6_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0457770.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACG8M6_002120"
     CDS             complement(967..2256)
                     /locus_tag="ACG8M6_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457771.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACG8M6_002121"
     CDS             2514..3458
                     /locus_tag="ACG8M6_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0457772.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACG8M6_002122"
     CDS             3472..4545
                     /locus_tag="ACG8M6_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0457773.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACG8M6_002123"
     CDS             4535..6706
                     /locus_tag="ACG8M6_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0457774.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8M6_002124"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG8M6_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0457775.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACG8M6_002125"
     CDS             complement(9009..11195)
                     /locus_tag="ACG8M6_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0457776.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8M6_002126"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG8M6_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0457777.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACG8M6_002127"
     CDS             complement(11417..11857)
                     /locus_tag="ACG8M6_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0457778.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACG8M6_002128"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACG8M6_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3519 a         2630 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa a
//
LOCUS       DAZKOB010000081        12444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000081 DAZKOB010000000
VERSION     DAZKOB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.081"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..177)
                     /locus_tag="ACG8M6_002129"
     CDS             complement(<1..177)
                     /locus_tag="ACG8M6_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0457779.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
     gene            319..933
                     /locus_tag="ACG8M6_002130"
     CDS             319..933
                     /locus_tag="ACG8M6_002130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0457780.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1122..3002
                     /locus_tag="ACG8M6_002131"
     CDS             1122..3002
                     /locus_tag="ACG8M6_002131"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0457781.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3081..3308
                     /locus_tag="ACG8M6_002132"
     CDS             3081..3308
                     /locus_tag="ACG8M6_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0457782.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            3313..3855
                     /locus_tag="ACG8M6_002133"
     CDS             3313..3855
                     /locus_tag="ACG8M6_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0457783.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4082..4798
                     /locus_tag="ACG8M6_002134"
     CDS             4082..4798
                     /locus_tag="ACG8M6_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0457784.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(4858..6693)
                     /locus_tag="ACG8M6_002135"
     CDS             complement(4858..6693)
                     /locus_tag="ACG8M6_002135"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0457785.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6889..7887)
                     /locus_tag="ACG8M6_002136"
     CDS             complement(6889..7887)
                     /locus_tag="ACG8M6_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457786.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8106..9326
                     /gene="sstT"
                     /locus_tag="ACG8M6_002137"
     CDS             8106..9326
                     /gene="sstT"
                     /locus_tag="ACG8M6_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0457787.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            9473..10108
                     /locus_tag="ACG8M6_002138"
     CDS             9473..10108
                     /locus_tag="ACG8M6_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0457788.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            10299..11039
                     /gene="truA"
                     /locus_tag="ACG8M6_002139"
     CDS             10299..11039
                     /gene="truA"
                     /locus_tag="ACG8M6_002139"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0457789.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11184..>12444)
                     /locus_tag="ACG8M6_002140"
     CDS             complement(11184..>12444)
                     /locus_tag="ACG8M6_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457790.1"
                     /translation="FSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRD
                     YQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTF
                     FLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLI
                     GAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFI
                     AYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAI
                     AENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVL
                     TTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSI
                     VTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4132 a         1991 c         2452 g         3869 t
ORIGIN      
        1 atcatataga tcatagatat ccttataatg tgtataaaaa gtcgaacgat gtacatctgc
       61 cgtttctgtt agttcgcgta tagtgattgc ttgaatcttt ttatttacta ataatgtgga
      121 tagtgtttca gccaaagctt tttctgtttt tttgacgcgt ctatctgtct ttttcattta
      181 cctatctcct tatccgacat atcaaagcaa agctgtcgct taatcgacaa acttatagat
      241 gattgattat agatagtaga cactgtgtct attataatgt aagtataaca caatcattga
      301 agttgaggag gcgaaatgat gaaactaact tttaaaccct tagaaaagaa agattacaaa
      361 aaagctatct attttgctgt tgaaggaatg aactttgaga gatatatgga aaatgaactt
      421 ctgctgaatc tttacggaag atattttttg tatatggagt tgaatcgagc aactcaaatt
      481 cttgctttgt atgcagagga cgagttggca ggtgttttgc tggcggagat aaaaggagaa
      541 aaaaagaaac atcgttcttt tggacagcaa atatatgtta agatattcga ttttttacaa
      601 aatatatttg ctaaagaaag taaaggagct tacgagacag caaatgaaca aatgcttgca
      661 gcatatttaa agaagcattc gccagatgga gagatcattt ttttagctgc aaatcctgat
      721 ttgaaaacaa aaggcatcgg taccaagttg ctaacagaat ttgaaaaaag agagaaagga
      781 aaagaaatat tcctttttac agatagtggc tgtacgtatc aattctatga acgacgtgga
      841 tttgaaagac taaaggaaaa aattagcaag ataaaaatct tagataaaga aatagatctc
      901 cgatgtatgc tgtacaggaa aatcattcaa tgaaaatttc gatagaaacg acaatagttg
      961 gcaagatcac ttttagtgag tcttcttgct aactattttt tgtagtgtat tctaccaaaa
     1021 gaaaaccgcc tttttgacta aacttgatag tggtcaattt tttatgatgg ataagtatct
     1081 atacttagtc tcgtaagaat gaaaggaaaa gagggagaaa gatgaaatta caaaagtgga
     1141 tccaagagca tcgaaatcat atcaccgcct tatcagctgt gttgattatt tttggtttta
     1201 tcggaaaatt tactggtttt gtcttgttct ataacattgc tctagccata gcttctatta
     1261 ttgcggtcat acctattatg atccatgcgt atcaggcagt gaaagtcaaa gttatctcaa
     1321 ttgatttact cgttactatt gctgtgcttg gtgcctttat gatcggagaa tacaaggagt
     1381 cagctatcgt tacattcttg tttttgtttg gtagctatct ggaacaaaaa acattgacca
     1441 aaacgcggga gtcaatcaag gctttgaccg agatggcccc atctactgct agtgttgtcc
     1501 aagaagatgg aacgactgaa gaagtggaaa tcgatgacgt ggaagaaggc gatatcgtct
     1561 tagtaaaaac tggtggagct attcctgtgg acggacgagt cattgacggg aaagggtatg
     1621 cggatgaagc cgcagtgact ggtgaatcac gacaagtcgt caaaatcagt ggagatgaag
     1681 tcttctctgg aacgatttta gcagatggct acgtgacgat tgaagcaact aaggtcggag
     1741 atgatacgac ctttgccaaa attatcgaat tagtagaaga agcacaagat accaaatccc
     1801 atgcggaaaa attcattgat cgattttctc agtattatac tccaaccgtt ttacttattg
     1861 cgttggttgt tggtctgatt agtcgtgatt ttcgtttagc aatcactgtt ttggttcttg
     1921 gttgtcctgg cgctttagtc attggtgcgc ctgtttcgaa tgtagcagga atcggtaatg
     1981 gagcgaaacg gggcatctta gtcaaaggcg gtgaagcaat ggatactttt tccaaaatcg
     2041 atacttttgt ctttgataaa actggtacat tgaccaaagg aactactgct gtatccacta
     2101 tcaagaacta tacagaggat gaaggggaca gcttagcttt agtggctcga ttagaaacgt
     2161 tatccgatca tcctctgggg cgcgccgtga tccattatgc tcaggagaaa gacattgatt
     2221 ttcaacaatt agcagtcaaa gataatcaga caatcaaggg acaaggaatc acagctgaga
     2281 ttgatggtca tacagtttgt gcgggcaaca ccaaattgat tgccgctcac aaactttctc
     2341 tcacaccaaa gcaagtacaa gatttacatc aattacaaca aacaggcagc tcggtcatta
     2401 ttgtagcaat cgacaaaaaa atcactcaaa tcataggtgt gtcagatgtg attcgaccag
     2461 aagtcgctga acaattagct aagttgaaac aagcaggagc aaaacagtta gtgatgctga
     2521 caggtgataa tcagatgact gctgattatg tagctgatat gttaggcatt gatgaagtcc
     2581 atgctgaatt attaccagac gaaaaggttc aatttgtgaa aaaataccaa gaacaaggat
     2641 tgcgcgttgc ttttattgga gatggaatca atgattctcc ctctttggct gcggctgata
     2701 tcgggattgc tatgggatca ggaacggatg ttgctatcga aacgagtgac gttgtcttga
     2761 tgcaatctaa ttttgaatcg ttggttcatg cctaccgctt ggctaagaaa actgttttaa
     2821 atacccgtga aaatgtagtg attgccattg gtgtcgtctt atttttactc aacggcttat
     2881 ttgccgggtt tatttacatg gctagtggga tgttcgttca cgaagctagt atcttgatcg
     2941 ttatctttaa cgcgatgcgt ttattgtatt ttggacacga aagcaaagaa gaaaaatcgt
     3001 aaaacttgat aagaatcaat ttttttatgg aagctgtaat ctagaataag actatcaaga
     3061 aaaaggaagg aagaactacg atgaaaaaaa ttattatgca attagataca ctagcttgtc
     3121 caagctgtat gcaaaaaatc gaacatgcgg tttctcaaca gcctggggta agcaatacga
     3181 aagttttgtt taatgcgagc aaaatcaaag caaactttga tccagatgaa gtccaccctg
     3241 aacaattagc tgcagttgtg gaaaaattag gttatgaagt ccaatcagca aaagtaaaag
     3301 aggcgtaaat caatgaatat agtggaagaa aaatttgttc aagaaacaaa acaagcagat
     3361 atcgaccatc ataaaccaac tgctggcgcc atggtggggc atgtattagc aaattttaaa
     3421 ttagaagaaa tgaaattgat ccaagcacaa ttgtatgttt tgggtacatc ttcgcaagaa
     3481 ttaagtaccc ttttttcaga atatgctgat caagaagcga gatggttcaa taaaatctct
     3541 catgagttag tgattgaaaa tgaaatcgtt ccgtctactt tagaagaagt catgcgttat
     3601 ggaaaaattt acgaacatgg tcaaaacaaa tatttaccag cctcaacaat gttggaaaac
     3661 tttgcaaaag attttgattt ccaaaatgtc tttatcacac gggcaatcaa attagctgaa
     3721 aaagaagaaa aatatggctt gcaacaattg cttgttgaac ttttaagttt caataaagaa
     3781 atcatctacc aagtacaaag actccaagga aaaactgtcc gacaagattt ggatgaggaa
     3841 gacgatgatg actaaaagtc tatgtttctt caaatacaaa tataaagcgt gaggtaaatt
     3901 ggaaaatcac gaagattaaa ataaaagaag cacccgctga tgtattacag acaacatcag
     3961 cgggtgcttc tcagttttta gaagatttga aagattgatg agaaaatacg aaaaatcaat
     4021 aaaatcacat tgcttatttg atagaataaa gaagtgggag taatcgaaag gagtttttta
     4081 attgaaaaaa aggattttag taacaattgg tctagctttt ttgttagctg gttgtaccaa
     4141 cgggaatgaa tcgaaaaaag aatcaacaaa agaaacagcg aaagcaacag ttgcaactac
     4201 tacaacaact agcacagaga gcaagtcaac tgcagcaagt acagaaacag cagatacctc
     4261 tacttcgtca gagtcttcca aaacgactga aacaactcaa acatctactt caagttcttc
     4321 tgtagaggct tcatcttcct cttcagaaag cagcgaacaa gttgaacaaa cactgtggaa
     4381 tgcagacaaa tccgcacaat tagaggcatt tatgaccaaa tggggccaaa caatgggaca
     4441 acaatacaaa agttatacga atcaaatgag tgtggactta tatggtctta aagtccctca
     4501 agctattttg aacggggagt ggaagatggc tattggtgga gtgcctgttt ctgcagaatg
     4561 gtcagaaagt gggacaggtc aagcagacta tcaaatcaca gccgtctatt ccgatgcaga
     4621 gacagaacca tatctgaaaa agcacgtcta tctattcggg tttcagcaaa atcagccaaa
     4681 agtattggtg acacaacaaa atcaggggaa tcccgataat tatctttact tcaacgagac
     4741 agcgaataat gaattgaaaa atggctttaa tcaaatcgtt tatggacaag caaattaatg
     4801 agataaacaa ggggttgtga catgtctttt atcacaaccc cttgctcagt ttatttgtta
     4861 gtcttttatt ttttgtgtcg cccatgttgc actgtactgg ttacctgcaa caagatcata
     4921 ttgtatcaga cgataatctt ctagaatttc tttttgtttc tcagttaaat ttttttcttg
     4981 gataatttga ctatcctctg taacaaagac ctgttttcca ttaaaacgat tagacaagct
     5041 ttctgtagga tcaattgtta atgcaggtag atgattagat acttctgtca acaacgcata
     5101 gtaaggagtt actttgatat tcgcatgctc aagagccata gcaccaaagt tattaggagc
     5161 aacaacttct ttatttaacg tagctgcttg ttcacgacta tacttgttgt tataaatgaa
     5221 ataatctgtc tggtgctgtt ctaatccata ttttttcgaa tcattcccac tatagattgc
     5281 gggtaaatgg tcaccataaa aaacaaaggt aatcggttta tcgattttgt cgatttcttg
     5341 gatgaatttt tttactgctg tatcggtata gtgaaggcct tgcatataag tttgtaattc
     5401 ttttcttcta ttaggaatga cagcagttcc ttcagcagtg tagttcaatt cactatagta
     5461 atcatcataa ggcatatggt tttgcatagt ggataattgg atgaattgtg acgtttccgt
     5521 attgctttgt aaagctttca aggtttcttt gtatgctgaa tcatccgata tgcgtgggct
     5581 ttgttctatt ttatccgtat aagttaattt atccggactg tctaaataat agaacctgtc
     5641 aaaaccaaat ttatcaaaca cttgttttct gcgataaaga gcagctgtaa aaggatggat
     5701 ggctattttt tcatcgaata gatcggtaat atttggactt attttctgac gctccactag
     5761 ctgggtataa ggtgtaacta acgaagcaga caggttactg atactcaagc ctgtcagtcc
     5821 ttcccattcc atgttagctg ttccgccacc ataaccgacg gataacatca atcctcctgt
     5881 tccttctgtt ataatgcttt tgatgtaagg aatagggtct gtaggtactg tcagattagg
     5941 gacacgtgca ggatcactga agctttcact taaattgaga acgatcgtct gattgtttaa
     6001 ccagtcactt cttgtttggt tgatttcatc tgcctttgtg ttgtactttt tcattattcc
     6061 attaattttt tctttcgaat agccagcagg ttcttccatg attttaatgt cgatattgtt
     6121 taggaattga atgactggtc cgttatcttt tacggcgcct tgctggttaa agaaagtctt
     6181 gtttacttta aataaattaa acagcaagaa agaaggcgaa tttttgtgat taatgaaaaa
     6241 gacacccgaa aaacatccta ataatacagc gatactgata aatcgacgtt tccaatgaat
     6301 cttcaaggaa tactgttttt gtagtctacg ttgtacaata atactagtaa tcaataatag
     6361 gagaaggatc aatccgccca cgattaataa gactgggcta accatcgata atagttctga
     6421 caatccggtc agcattttta aatctgaagg aaagacaggc tcttcccgca tttccatttt
     6481 gattactgtc gaaatagtga gcagtaagtt aatgatcaag gtagcagtca aggcgtacca
     6541 aaaacgattg gaaatcaaca atagtaagaa gaaaaatccc ataataatga tgattgtcag
     6601 gatcatgggt gcttgtcgct gagtaaatat ttttaaggca acctggatag tttctttcca
     6661 accttctttt ggttctaaaa cgaaaatctg cacgaaggta aagcagtaaa aaaatgctgc
     6721 aactaaaaaa taacttttat gaacaaaaag ccaatgaatt ctttttttcc aacgctgagt
     6781 taattgttca atcgaatcaa aagcgaagta ttcgatgaaa cgatgaatga aacggatttt
     6841 ttgtatataa aataaaaaga taactaggag ggacactgct aataaatatc aaataaaaaa
     6901 ctgcccgata tttatcagcc atgccttccc tgagtttgga aattcacgtt tcataaatgt
     6961 agatacgcga taagaaacta aagggaaatt acatataact tgagtgacaa tcaaaaatac
     7021 agcaagaaaa tttcctcttc cttttatacc taatttatca gatagcattt ctaaaaataa
     7081 aaacgaagag aaagtataat acattgcgaa gagagaatat ggtttagaaa acctcagcgc
     7141 tgtatctgta tttcctctga tgatgagtga aatttgtgtc atcaaaataa tcaaaccgaa
     7201 taaaacaatc aagctgataa atacttgagg aagcttatag cggacctttt ctttccagtt
     7261 ccattctttc aaaccataac ccattaaaaa taaataaaaa atccataaaa tactctgtcc
     7321 attctgtaat gcaaacgggt ctttattaaa tagagtagag cagccaacta atccaattgt
     7381 taaaaataat agtagatatt taattgttgt tttcgataat ttatttaaaa atccaactgt
     7441 ataaggaata ctacaaaaaa gcaaagtaca agaaacagca taagtaaagt aattctgact
     7501 gataggtgtt aggataagcc aataatcacg aatattcaag ctgccagctt gtacgtaaaa
     7561 aaagatattg caaactagtg tggctattag gaatataaag gcaaaacgac ttaaataatg
     7621 taaaactttt aaacgggcga ttttttgata ttggtaaccc atgtaaagtg ggatcaaatt
     7681 tgcaccaata acaacggaag tttgtaaaaa tgtatagatg tagaatcgac cagggtttgt
     7741 tgcatatgta ggaatagtga ctgctgaaat caatatacgc tgtaaaacca tcattataca
     7801 agcgacaaag attgtcagga agccataact ctccttcttt atttttttca agaataaaca
     7861 cctcacaaaa aaattggaca aaaacataag aaaacgtttc ctgataagtt gcccattaat
     7921 aacatcctaa cttagaatat ctcaaaaaac aacgtgtaga cagaaaaagt acaaaattag
     7981 caagaaatgg ttgtttgtat tgttagaatt tttagaaaat tataccttta gtttctttgc
     8041 ataaaatgat acaatgattg ttaaaattgt gaaagggtgg tcatttgtta gagaagtttt
     8101 tggatatgtc gttagttcaa cgtattgttg ttggtatttt agtcggaata ttgtttggta
     8161 tctttttccc cacatggaca tttatttcaa tattgggaac gttgtttgtc agttgcttga
     8221 aggcaatcgc acctattctg gtttttttct tgacgatggc gtcaattgcc aagcataaaa
     8281 ttgggaataa gacattcgtg aagcctattt tgatcttata tttagtggga acattgcttt
     8341 ctgcacttgt agcagttatt gtaagttcaa tttttactgt tcctattact ttacaagaaa
     8401 cagtatctga aaaagcaccg caaagtttgg aaagtgtttt atctactatg ttgacgaatg
     8461 tcacacaaaa tcctgttcaa tctcttattg atgcgaatta ccttggcgtc ttattctggg
     8521 ctgtattgtt gggactagct tttcgagcac gttcagaatc gacaaaagag ttggtggatc
     8581 agatatctat agcgttgtca caagtcgtac aagtgattat tgcatttgct ccgataggga
     8641 ttttaggatt ggtttatcaa tcaattgcta cgactggaat cgctggattg gcagagtatc
     8701 tgcagctttt acttgttttg atcggtacga tgctgtttgt cgcattagtt gtgtacccat
     8761 tcctcacgtt tttgtttatt aaagaaaatc cctatccatt gattttcttt tgtttgaaag
     8821 aaagcgcact tcccgctttc tttacccgaa gttctgccgc aaatatcccg ataaacatga
     8881 tgttggctga aagattgaaa ttgacaaaag agtcttattc catatcgatc ccgttaggtg
     8941 ctacgatcaa tatgggcggt gccgcgatta ccatttcctt gatgacgttg actgctgtac
     9001 attctttagg gattgaagca ccttttgttt tgaaagtcct tttatgtatt ttgtctgctt
     9061 tagctgcttg tggagcttca ggtgttgcgg gcggttcact gttattgatt ccattagcat
     9121 gtagtttgtt tggtatctct aatgatgtcg cgatgcaaat cgtcggtatt ggatttatta
     9181 tcggtgttat ccaggattca atggaaactg ccttaaattc gtcaagtgat ctgttgttta
     9241 ctgcaatagg agagctaggc gcgcgtaaaa gaagtgggga aaaaatcaaa ttgaaagatt
     9301 gtttagatgg attaagtgaa cgttgaacgt tagatattta gtatttttaa aatgattcga
     9361 gtttttaatg agttatatag atgttcgtgt aaatagaggt ttctgttaaa tcaagataaa
     9421 gtcgtttata ataacaatta aaagcatagg taattgttat tggaggcgta tcatggattt
     9481 tttacgtaaa tttggttttt tctttttatt attaggcgtg ctgagtgatt ttttgactcc
     9541 gtacgtatta ggattgtatt atcctgaatt agatcaaatg aagagcgtga tcagtgtatt
     9601 tggagaggtt tccagtcctg taagaaaagc gtttcttatt tggtctgtgg tttcagggat
     9661 ctttttcatc ctgtcattgc ctgcattgta tgctgcattt gttagtacat ctcgtgtgtt
     9721 agctacccta gttacagcag ccatcgcgtt ctatggtata ggtgattgta tctttacagg
     9781 tttgttcagt atcaataccc atgaatcttc gtggaatgtc tcgacatggg tccataatat
     9841 cggatcaggt ttaggttaca gtggtttttt attgtttcca gttcttcttt tcttgatcta
     9901 tcgaaaaaat gggcaaatgg acgtaagcca cttatacctt ggactgtctg tgatcagttt
     9961 gctttttgcg gcgatttatg gactggcaag aattccttct ataaattctt ggccattact
    10021 gaataaaata ggtttttgcc aaagaatcag ttttttcttt aattatgtgc ccattgcctg
    10081 gttgagtatc ttgcatctca aacagtaaaa cttgagccgt caaattgaac agtcagcgga
    10141 tacttcatcc ggtggctgtt ttttgttttt ctgaaatata tatttccaca gttttcttgt
    10201 atagcttctg tcttagagaa gcaaggagct atattttgtg ctacaatctt aaaaaggtag
    10261 agagaagacc aaatggaaga cagaggagcc actaaaatat gagaaacatc aagttgacaa
    10321 tcgaatatga cggaaaaaga tattcaggat ggcaaagact tggagatgac gataaaacca
    10381 ttcaaggaaa aatcgaaaaa gtattgcatc agatgacaaa tgaagagata gaaattattg
    10441 gatcaggaag aacagatgct ggcacccatg cgcgaggaca agtagcaaat tttaaaacaa
    10501 acacagaaat tggactggca gaaatgatcg actttatgaa tcgttacctt ccgcgggata
    10561 ttgtcatcaa aagaatcgaa gaaatgcctg agcgcttcca ttcaagatat aatgcggtgg
    10621 gcaaaaaata cagctattat gtttggaaca acgtgattcc ttcagcattt gagcgaaacc
    10681 acagctttta tttccctcaa gagttggata tggacaagct gaatgcagct tgcgaaaaat
    10741 tagtgggtac acatgatttc attggttttt ctgcattgaa aaaatcaaaa aaatccacag
    10801 tgcgtacgat tgatgaatta tctatcgaac gaaagggaga aatgcttcat tttacttttg
    10861 tcggagacgg attcttatat aaaatggtgc ggattatcat ggggacatta ctagaaatcg
    10921 gcacaggtgc catgccgata gaaacaatcg atgaaatatt tgaaagcaaa gtgcggcaac
    10981 atgcaggaga aaccgtccct gcacaaggtc tgtttttgga tgaagtttat tatcgataaa
    11041 aatactttga taatggatga aacgcatgtt tcacagtata aatatcgatg ttaaataatg
    11101 tatgaagcaa attaaagaaa tataaaaaaa gactttttag cctgtttatg tgtacagcta
    11161 aaaagtcttt cttaaataaa aatctagtga gaatgttgta cttctcttct cggttgtttt
    11221 tgtttagaaa aagtaaatcg ataaagtaaa aataaaatca aagtaacgat agataaatat
    11281 cttgaagcaa ttgctagttt tgtgtactga taagtaataa cgactttatt tatacctgga
    11341 ggaatagtta attcagttgt gccaaactta gataattttg tttgtactag tttgccatta
    11401 atagaagcca cttgcccttt ataacgataa ataggtgtcg tcaagacagt atttttatta
    11461 ttcgaattat ctaattcaat tgtaagtttg ttactcataa aatgagttgt tggatttatt
    11521 tcctggttac ctagtaaata ttgatcatta ttaatcatat caggatgata gattgactct
    11581 ttgtttgcat aatcagtatg attgtaattt tcagctatag caacagcgtc ttcaggtgct
    11641 ataagtgtta atgaatcttt gttagcatgg atagaattgc ttactcctga gagatgcaat
    11701 attagtgcaa gaaacgtaaa gaccattatt ttccatgaat tatttttagt agaaaaaata
    11761 taatagatca ctatactcat cgtataggct ataaataaag aagaaaaacc atttaaccgc
    11821 cagacaaatt gtagactctt aactggtgta tggttaaaaa gttgccaagg aaataaattg
    11881 gttgaacaaa aaagaacaaa tacaccaaga aagaaaataa ataaatcatc agcagttaat
    11941 ttttttataa aaataaaagc accaatcaat ccgagaaata agaataatcc aatagtatag
    12001 ttgttatagt tattatttaa aatatgtgtc agcatatcac taggagccat accacttaat
    12061 tcttttccca gtgggacttt caattcttgt gctctagatt gttgaataaa aggaattaag
    12121 aatcccgcac ttaaaaaaaa ggtgaggacg gtagctttta aaagacttaa gagtctagcg
    12181 attttttggt cccaaaaata aaaagaaaga aacaacgttc ctccaataaa tacagaaacc
    12241 atagcgacag aaagtaaatg agtatacact actagcgtca tgccaataga caaccagtac
    12301 catttttggt aatctctaat ataaatttcg taacacccca ttagtatcaa aggcaaaaat
    12361 gttaaagcga cagcttctcc caatgaagca cgatggaata tgtcaatggc acgataggcg
    12421 gaaaatgtat aaataatcga aaat
//
LOCUS       DAZKOB010000082        12426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000082 DAZKOB010000000
VERSION     DAZKOB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.082"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..646
                     /locus_tag="ACG8M6_002141"
     CDS             <1..646
                     /locus_tag="ACG8M6_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0457791.1"
                     /translation="LVKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNS
                     EISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVIS
                     NIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHF
                     RKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            718..1839
                     /locus_tag="ACG8M6_002142"
     CDS             718..1839
                     /locus_tag="ACG8M6_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0457792.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(1921..3693)
                     /locus_tag="ACG8M6_002143"
     CDS             complement(1921..3693)
                     /locus_tag="ACG8M6_002143"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457793.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3696..5408)
                     /locus_tag="ACG8M6_002144"
     CDS             complement(3696..5408)
                     /locus_tag="ACG8M6_002144"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457794.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5523..6191
                     /locus_tag="ACG8M6_002145"
     CDS             5523..6191
                     /locus_tag="ACG8M6_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0457795.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6258..7046)
                     /locus_tag="ACG8M6_002146"
     CDS             complement(6258..7046)
                     /locus_tag="ACG8M6_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0457796.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7129..8601)
                     /locus_tag="ACG8M6_002147"
     CDS             complement(7129..8601)
                     /locus_tag="ACG8M6_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0457797.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8731..9924)
                     /gene="metK"
                     /locus_tag="ACG8M6_002148"
     CDS             complement(8731..9924)
                     /gene="metK"
                     /locus_tag="ACG8M6_002148"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0457798.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(9922..10001)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10190..10261)
                     /locus_tag="ACG8M6_002149"
     tRNA            complement(10190..10261)
                     /locus_tag="ACG8M6_002149"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10226..10228),aa:Glu,seq:ttc)
     gene            complement(10273..10346)
                     /locus_tag="ACG8M6_002150"
     tRNA            complement(10273..10346)
                     /locus_tag="ACG8M6_002150"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10310..10312),aa:Ile,seq:gat)
     gene            complement(10359..10429)
                     /locus_tag="ACG8M6_002151"
     tRNA            complement(10359..10429)
                     /locus_tag="ACG8M6_002151"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10395..10397),aa:Gly,seq:tcc)
     gene            complement(10434..10506)
                     /locus_tag="ACG8M6_002152"
     tRNA            complement(10434..10506)
                     /locus_tag="ACG8M6_002152"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10471..10473),aa:Phe,seq:gaa)
     gene            complement(10510..10582)
                     /locus_tag="ACG8M6_002153"
     tRNA            complement(10510..10582)
                     /locus_tag="ACG8M6_002153"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10547..10549),aa:Asp,seq:gtc)
     gene            complement(10601..10674)
                     /locus_tag="ACG8M6_002154"
     tRNA            complement(10601..10674)
                     /locus_tag="ACG8M6_002154"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10638..10640),aa:Met,seq:cat)
     gene            complement(10687..10776)
                     /locus_tag="ACG8M6_002155"
     tRNA            complement(10687..10776)
                     /locus_tag="ACG8M6_002155"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10738..10740),aa:Ser,seq:tga)
     gene            complement(10799..10870)
                     /locus_tag="ACG8M6_002156"
     tRNA            complement(10799..10870)
                     /locus_tag="ACG8M6_002156"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10835..10837),aa:Glu,seq:ttc)
     gene            complement(10892..10979)
                     /locus_tag="ACG8M6_002157"
     tRNA            complement(10892..10979)
                     /locus_tag="ACG8M6_002157"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10943..10945),aa:Ser,seq:gct)
     gene            complement(11004..11077)
                     /locus_tag="ACG8M6_002158"
     tRNA            complement(11004..11077)
                     /locus_tag="ACG8M6_002158"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11041..11043),aa:Ile,seq:gat)
     gene            complement(11091..11161)
                     /locus_tag="ACG8M6_002159"
     tRNA            complement(11091..11161)
                     /locus_tag="ACG8M6_002159"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11127..11129),aa:Gly,seq:tcc)
     gene            complement(11163..11238)
                     /locus_tag="ACG8M6_002160"
     tRNA            complement(11163..11238)
                     /locus_tag="ACG8M6_002160"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11203..11205),aa:Asp,seq:gtc)
     gene            complement(11257..11330)
                     /locus_tag="ACG8M6_002161"
     tRNA            complement(11257..11330)
                     /locus_tag="ACG8M6_002161"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11294..11296),aa:Met,seq:cat)
     gene            complement(11343..11432)
                     /locus_tag="ACG8M6_002162"
     tRNA            complement(11343..11432)
                     /locus_tag="ACG8M6_002162"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11394..11396),aa:Ser,seq:tga)
     gene            complement(11456..11529)
                     /locus_tag="ACG8M6_002163"
     tRNA            complement(11456..11529)
                     /locus_tag="ACG8M6_002163"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11493..11495),aa:Ile,seq:cat)
     gene            complement(11543..11616)
                     /locus_tag="ACG8M6_002164"
     tRNA            complement(11543..11616)
                     /locus_tag="ACG8M6_002164"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11580..11582),aa:Met,seq:cat)
     gene            complement(11644..11716)
                     /locus_tag="ACG8M6_002165"
     tRNA            complement(11644..11716)
                     /locus_tag="ACG8M6_002165"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11681..11683),aa:Ala,seq:tgc)
     gene            complement(11736..11809)
                     /locus_tag="ACG8M6_002166"
     tRNA            complement(11736..11809)
                     /locus_tag="ACG8M6_002166"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11773..11775),aa:Pro,seq:tgg)
     gene            complement(11819..11892)
                     /locus_tag="ACG8M6_002167"
     tRNA            complement(11819..11892)
                     /locus_tag="ACG8M6_002167"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11856..11858),aa:Arg,seq:acg)
     gene            complement(11903..11988)
                     /locus_tag="ACG8M6_002168"
     tRNA            complement(11903..11988)
                     /locus_tag="ACG8M6_002168"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11952..11954),aa:Leu,seq:taa)
     gene            complement(12005..12076)
                     /locus_tag="ACG8M6_002169"
     tRNA            complement(12005..12076)
                     /locus_tag="ACG8M6_002169"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12042..12044),aa:Gly,seq:gcc)
     gene            complement(12088..12160)
                     /locus_tag="ACG8M6_002170"
     tRNA            complement(12088..12160)
                     /locus_tag="ACG8M6_002170"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12125..12127),aa:Thr,seq:tgt)
     gene            complement(12178..12259)
                     /locus_tag="ACG8M6_002171"
     tRNA            complement(12178..12259)
                     /locus_tag="ACG8M6_002171"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12223..12225),aa:Leu,seq:tag)
     gene            complement(12267..12339)
                     /locus_tag="ACG8M6_002172"
     tRNA            complement(12267..12339)
                     /locus_tag="ACG8M6_002172"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12304..12306),aa:Lys,seq:ttt)
     gene            complement(12343..12415)
                     /locus_tag="ACG8M6_002173"
     tRNA            complement(12343..12415)
                     /locus_tag="ACG8M6_002173"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12380..12382),aa:Val,seq:tac)
BASE COUNT         3967 a         2683 c         2206 g         3570 t
ORIGIN      
        1 tttagtgaaa attaccgaag aaaaagaaca acaaaaagca ataaaagaac agatgataga
       61 agaggaaaaa gttcgtagag aaattgatcg tgaaaagaaa cgtattgata aagaacaacg
      121 tcaattcaat tctgaaattt ccaaattaat cgcatatatg caaaaagcta atgcagatgt
      181 ggaaaaagaa ttatatgcaa ataaaattca agaactggaa gaaaaattaa aagaattaga
      241 agttatacgt gaaaatgtat tacaaagaga attaaatact agagccggtt atgtgtatgt
      301 aattagtaat attgggtctt ttggtgaaga catatataaa attggtatga ctagaagatt
      361 agaacctatg gatagaataa aagaattgag tagtgcatcg gttccatttg aatttgacgt
      421 tcatgcgatg attttttcag aggacgctcc gtctttggaa actaaattgc ataatcattt
      481 tagaaaacaa gaagttaata aaattaatca aagaaaagaa tttttcaaag tttcactaga
      541 cgaaatagag aaagttgttt tagaaaatta taatggaaca gtaacattta caaaattagc
      601 taaagctgaa caatatagac gatctttaga attgtcaaaa gattaaatat ataaaaagcc
      661 ttcgggcttt ttcttttaac aattaatagg aacgtatatt cgaaaggaat agaaataatg
      721 tggttagaaa aaataaatga caaaaaattt aagtacaccg aacgttatac ggatccctta
      781 actgaaaaaa agagaaaagt gtccgttact ttatctagca attcacgcca agcttggaat
      841 caagctaact tacttttgaa tgaaaaaatc gcagaaaaaa taaaacgaaa tgaagaatta
      901 ccactaactt ttggagaatt aaaaactaaa tgggatgaaa aatacaagcc aactgtaaaa
      961 gaaagttcct atcgaacaac tcaggtttat ctatcattaa tttcaaaata tataaaagat
     1021 gatgtacttg taaaaaacgt taatagtaat cttatacaag atatgctaga ttactactat
     1081 tttgaaaaaa atttatcata taattatgta atacatttaa aaacttttac aggaatgatt
     1141 tttaaattcg ccaaccgtcg atatggatta aaatataatc ccgtaaatgg ggtttcttta
     1201 gcaagaaaac ctaagacaaa cgaagaaata aaaaaagaaa aagatggtta tttatcaaaa
     1261 gaagaagttc aaatgattgc tagtaaacaa aaaagttcac atcaacagtc acgttattct
     1321 ttaattacta aatttttgtt tttaactgga ctacgttatg gtgaattgat atctttgact
     1381 gaatctgatt atgatggtaa taaaattacg gtttctggta cttatgatta cgagttaaaa
     1441 attaaaacaa caactaaaaa tacaggttct tatagaacca ttgaattatc aagtaatgct
     1501 aaagaaatca tagatcaatt aattgaagaa aacaaattaa ttaagaataa taaagataaa
     1561 tacattttca tctctaaaaa tggtaacccg atatctattc aagcatataa tcaatcttta
     1621 agagcagtat ccaaagaatt aaacctagat aaaaaagttt cttctcacat gttaaggcat
     1681 tcccacattt ctctacttac agaattagga atcccattaa aagccattat ggatcgtgta
     1741 ggacatgaag attcaaaaac aacattaaag atttatactc atacaactaa aaatatgcaa
     1801 aatcaactag tagaaaaact aggaaaaata gaaatttaaa ttgccccttt tctgcccctt
     1861 ataaaaacaa acaacataaa atatataaaa agaaccccgt tataacgagg ttcttttaat
     1921 ttaactttca aatacaaatt gattatgata aagttccgca taaaatccat tttctgctaa
     1981 taactcgtga tgactgcctt cttcaatgac ttccccatca cgcaagacaa cgattctatc
     2041 tgcattcaag attgttttca gccggtgagc aatcacaaag ctggttcttc ctttgattgc
     2101 ttcatccatt gctttttgga tccttgcttc tgttaccgtg tctacattac ttgttgcttc
     2161 atccaaaatc aataaagatg gatttgtgat gatcgttcga gcaatactga tcaactgctt
     2221 ttgacctgta ctgaataagt tattttcttc ggatatctct gtatcataac ctttttctaa
     2281 cgtcatgatg aagtcatgaa tattcgcttg ttttgctgca ttgatgactt cttcatctga
     2341 agcgtccggt ttgccgaatg tgatattgtc tttgatcgtt ccggagaaca atacagattc
     2401 ttgcaaaacg attcctacat gtctacgtaa tgcatcaaga tcataatctc ggatatctat
     2461 tccatcgatg actactgaac catcattgac atcgtaaaaa cggtttagca gattcatgat
     2521 cgtcgttttc cccgatcctg ttggaccaac aagagcaacc atttctcctt tgtccacatt
     2581 gatcgagaca tctttcaaga ctagtttttc tggagaataa ccaaaatcta cgtgctgcaa
     2641 cgcaagacct ttttcaacac catcaaatgc tttacagttt tctggtttga cttcatcttt
     2701 ttcatcaaat atttcattca atcgtcttgc ccctgtaatc gctaattgga tcatgctgta
     2761 tccagatgaa atctgcatca gtggttggta atattgttgt gaatactgga cgaatgtcac
     2821 aactaagcct aatgccaccg aacgttccaa atcaccattt aatgctaacc agccgccgaa
     2881 aaagataact atcgctgtat tgaccagtga cattccttgc atcatcggaa atagtagacc
     2941 agaataagct tgtcctttga aggttgcttc gcgaacactt tgattatgct ctaagaatcc
     3001 ttcaatcgtt tcttcttgta agccatttgt gatgatgaca cgttgtccgc tgattttttc
     3061 atccatataa ccatttagtt tccccacttc atcttgctga agatccacat atttccgtgc
     3121 tttgcggata atcaagacag ctatcaaaac ggctactgga gtggaagcaa ttgtagccca
     3181 agctaactct acattttggc ggaacatcat gatcaagatc ccgacaagta aaacagcatt
     3241 cgtgatgact tgaagcaaag cttggttcaa actattttga atattatcta agtcactggt
     3301 aaaacgactt aaaatttcac cgtcttgatg agaatcaaag aaacggatcg tcaatttttc
     3361 caatttgttg aatagaccga tacgcattcg gttcgttgac ttccctacta cttgtgtgaa
     3421 caaaatacta tagataaagt tggctgcact agctagtaca taaaacagca agagtttcca
     3481 tattacagaa acaaatttgc ttttatcgtc tacgccttgc atcaagcctc ctacgtaatt
     3541 agctaattct tgaaaagctt ccccgatgta ttgaggtgcc tttacttgta gataggtagc
     3601 aatgatcact gtgataaaga taaagaaaaa ggagagtttg taccgcttca aatagtgata
     3661 gaaaaattta cttgctttta ttagatcagt catacttact cctccgttcc tttttgagtt
     3721 tcaaagattt cctgatagac acggtttcca gctaccaatt cctcatgggt tccttcgccg
     3781 actaatctgc cttcatccaa tactaagatg cggtctgctt tgacaacaga agcaattttt
     3841 tgtgcgatga caatcgtcgt tgttcccttc aaatctcgat cgagtgcttc tcggaccagc
     3901 ttttcagagc gagcatccag tgcactcgta ctgtcatcta ggatcaagat ctttggatta
     3961 ccgataacgc cacgagtaat ggataaacgt tgcttttgac caccagaaaa gttactgcta
     4021 cgttcttcta ttggtgcttc gtaacgatca gataactttt cgatgaattc ttttgcttga
     4081 gcaatagaag aagcatgttc catctctttt tctgttgcat tctttttccc ttggcgcaag
     4141 ttttgagcaa tcgttcctga gaataaaatt gctttttgca aaacaaagga aaccgtttgt
     4201 cttaaactct tttcatttac ttgacgaaga tctacaccac ctacttcaac tttgccttct
     4261 gtcggatcaa atagacgagg gatcaattgt gctagtgttg acttcccggc accagtcgcc
     4321 ccgacgatac caaccatttc tccaggtttg atagaaaagc tgatatcttt aagcgtatct
     4381 gtatcctcac caggataacg aaaagaaaca tggtcaaaag tgacagaacc cgtcaactct
     4441 tgttctggaa cgtctaaata agtaagatct ggttcagcat ctaaaatttc cgcaatccgc
     4501 ttcagcgata cggctgctct tgaagtcatc atcatcatca ttccgccaat aatgattgcc
     4561 atcataatct gcatcaagta gttcatgaat gaagcaattc caccaattaa cgtaggatca
     4621 tctttaacaa gatcgctcac aaaaaagatc gccccaacca cagccaaatt ggcagccagc
     4681 atgaaggacg ggatcatcac agaaaataat gtaccgacta tgatgttgtg ttttgttaag
     4741 tcctcactta ctttgctgaa tgacttcaat tggttttctt cttgtacaaa agatttcact
     4801 acacgaatcc ctagtaagtt ttcttttgca atgctattca cacgatcaat cagtttttga
     4861 atgatcatga agtgcttgcc catccttgta aatgaaagtg ccgtaatcag aaataccgta
     4921 acgataagcg ctacgatgat ccaccataat tgcggcatcg tgatcatagc taaaatgaag
     4981 gcaccaataa ataagaatgg aattcgaaaa agtgattgca aagaaatcat cactaaattt
     5041 tggatttgcg tgatatcatt tgttagacga acaacaagat ttccagcaga gaactgttcg
     5101 atatttccaa atgaaaatgt ctggatcttt cgaaatgctt cttctcggat atctgcactt
     5161 acgccttgag ctacttttgc agagaaaatc gtgttcgtta cacctgcggc taatcctagc
     5221 aaagccagac tgatcaggta aataccaatt gttttcattt catcattatc ttctgtaata
     5281 atagcttcca atacttgctg tagtaactta ggttgccata atgctgctgc aaccatgact
     5341 gctacggaca gcaaggaaat aaaaacagct actttatatt ttttcgcatg tcgtaagatt
     5401 aatttcattc gttgattaca cccctttgtt taaattgaca ggactaattt aacactgtaa
     5461 aattagaccg actaacaatt taccatgttt aaaaagataa tgcaatacac gaggttgata
     5521 aaatggaaat aaagctttct caagcaatca tcattcaggc cgcctttgat atcctggctg
     5581 aaacacaagc tctttcgcag ctttccatgc gaaacatcgc aaacagaatt catgttcaag
     5641 cacctgcttt atattggtat ttcaaaaaca aacaacagct cttgcaaata atggctgaaa
     5701 caatggaaga agaattagcg cttccagatc caactttgcc ttggaatgaa cagttgctgc
     5761 agtatatgga aaattactat gacctttata cacgtttccc ttgtggggca gaattagaaa
     5821 tcaatactgt gcccgctttt ccgagtcgat tagaacattt ggagcaaatg atccaattac
     5881 ttgttcgtga aggattttca gtcacccaaa gtcatcaggc cattgcctct ctccacaatt
     5941 tattgattgg acagttaatg gatcagcagc atgaacagca gcttcgacaa aaaattatgg
     6001 acggaaatga atttttgaaa gaaacagttc tatttatgcg taattatgta gttgaaaatc
     6061 aattagaggg atttaaagaa agtatgcagt cccatgcaga attgaatgaa aaagcactct
     6121 ttctaacgag tgcacaaacc tatattgaag gcttatgcca acaaaataaa agaagagaaa
     6181 acgagcagta aaaaagctcc tatagagatc actatgtttg tgatctctat aggagctatc
     6241 tttttaagac tcttttttta tttttcttcc attagccacg ccaatgcacg ttgaatccat
     6301 tggtcccaga agatccagtt atgatcgcca ggtccttgtt caaaggtgac cgccaagcct
     6361 tgttcctgca tagcagatac catttcctga tttgcctgat aaagaaaatc agattcccca
     6421 caagccatat aaaatcttgg aactggtttt ccgcttttta ctagttcttt caataagtat
     6481 tgcggatcat tttttgaacc ttcgatttga tccaatggtc caaagatccc ttcccaaaaa
     6541 gatgctttac gaaccatcaa aagatcatcc attcgatttc ctacagaaac agcgcctgaa
     6601 agagagatta ctgctgcgaa attttctgat tttgcaaggc ctagttttaa cgcgccatat
     6661 ccgcccatcg ataatccagc ggcatatgtt ttttctcttt ttcttgttaa ttgcgggaat
     6721 aattcatgac aaatctcagg tagctcttct gcaatgaatg tccaatactt catgtcataa
     6781 actgtatctg tgtaccagcc aagatcagta gatggcatga tgacagctaa tccgtagttt
     6841 gctacataac gttcaatcga cgtacgtctt tcccatacac tatgattgcc acccattcca
     6901 tgaagtaaat ataaaacagg tatatccgtt gtctcttgct ctgaactggt gccaatcacc
     6961 ttttttgttg tttgcggtag tatcacattt agattgactt ccatttctaa cacattcgaa
     7021 taaatatttg cttgaataaa tgccatattt tcgccttcct tagtaagaag ttatttttga
     7081 ataactttaa atttgaatga ttcggctacg tacatatctt aaccgaaact actgttttat
     7141 tttaccactt ttttcatgaa cgaaaagcgt taagaccaaa gcaatgatca cgagtaacaa
     7201 gccaactaga taaacggaat gtaacccatc ataaagaatt tttcttaatg gtaacaaaat
     7261 atccgcatca atatatttag ccgtttgtgg attcaccagt ttattcatga tatctgcatc
     7321 atttgttaaa tttgctgctg caagttctcg tttagttacc ccatttatca ataaaccaaa
     7381 aatagaaacc atgactgttt gaccaattgt tcgaactaac gtattaaaag aagttgctac
     7441 accaagctgg tcacgagata cactattttg tgcactgaca gtcgaggtag tcatggtcac
     7501 acctaagcct ataccgatga ccgttgatat agcaaaaaac cataaatagt tacttctgaa
     7561 aggcataaga tacaaccaag cgcccccaat agctgcgatt agtagtccaa ttctcaagac
     7621 ccaatgagtt ttcatttgag tcatccactt gcttgcaagg aaagaaccga ccatccagac
     7681 tagtgataat ggtgctaata ccagcccacc aattgctgca ttcagcccta atacaccttg
     7741 catccacata gggatataaa catcgattcc catcaaaaaa ccactagcca atgctgcagc
     7801 caagttgacc acaacaaata gtggattttt gaataaatgc atcatgatga caggatcttt
     7861 agctcttttt tcggcaaaaa caaaaatacc taacattacg atactaccta gcagcaaagc
     7921 agaaacaagc ggactcattc cttcgtctcc taataattgg aaccctaaca gcaatgagac
     7981 aagcatggcc atcaaactaa aactaccaaa aatatccatt ggtttagact catgtatgat
     8041 ttttggttca tttaaatata gccaaatcaa aataattaat agaattccga tcggcacatt
     8101 gataaaaaag atccagtgcc agctgatcgt ttcaacgatg aatcctcccg caagcggtcc
     8161 aaaaacactg gcaatccccc aggctgaact attcaaacct aaaacttttg ctcgcttttc
     8221 aatagaataa agatcgccga tgatcgtcaa agctactggc atcattgccc cagcacccat
     8281 cccttgaatc gcacgagcag caatcaaccc aatcatgctt tgagataaac cgcaaaataa
     8341 cgaaccgata ataaacaata atgtcccaaa aataaagatt ggtttccgtc ccactttatc
     8401 cgcaagtttt ccgtaaatcg gagtaagcat agcgtttgtc agcaaataaa tcgaaaatac
     8461 ccagttcatg atttccattc catgtaatga accgacaatc gttggcatag cggttgttac
     8521 gattgtacct tcaatagccg tcatgaacgt tgcaatgaaa atactcactg tgaccaaagt
     8581 aacatttgtt tcttttttca tcttattcct ccctttcatt atgacaggat ctaaaaatag
     8641 ataaataatt actatctttt tcaagaggtt aactgccata aaaaaagctg gaacaaccac
     8701 cacttggcga tcgttccagc tgaatataac ttattttgct aaacttgctt tcaacgcatc
     8761 tactttgtcc gtatgttccc aaggcaaatc tacatctgta cgaccaaaat gaccgtaagc
     8821 agctgtttgt ttgtaaatcg gacgacgcaa atcaagcatc tcgatgattc ctgctggccg
     8881 aagatcgaag ttttctctca ctgcagcaat cagttcttct tccggaaccg ttccagtacc
     8941 gaaagtattg attgagatcg aaacaggttg agcaacacca atcgcataag ctagctgtac
     9001 ttctgctttg cgagcaagcc ctgctgcaac aatgttttta gcaatataac gtgcagcata
     9061 gctggcagaa cggtcaactt ttgtcgcatc tttaccagaa aaagcaccgc caccatgacg
     9121 agcataaccg ccatacgtat caacaatgat ttttcttcct gttaatccgg catccccttg
     9181 aggaccacca atgacaaatc ggccagtcgg attgatataa tatttcgttt gatcatctaa
     9241 taattcagct ggaataactt ccttcactac tttttcgatg acatcatgac gaattgtttc
     9301 attatctact gcatcatcat gctgtgttga aataacgatt gtatctacgc gttccggttg
     9361 tccttgatca tcatattcaa ccgttacttg agattttgca tctggacgta aatacgttaa
     9421 ttcatttgat ttacgcaaat ctgccaagcg tcgtaccaga cgatgactca aagcgattgg
     9481 taaaggcatc aattctggtg tttcatctac agcaaaccca aacattaaac cttggtcacc
     9541 agcaccaatc tcatctaata cgtctttttt atcttcatcc cggatctcaa gtgcttcatc
     9601 aactccttga gcaatatcag gagattgttc atcaatcgca accaatacag ctgctgtatc
     9661 tccatcaaat ccgaattttg cacgtgtata accaatttct tttattgttt cccgtacgac
     9721 tttttgaata tctacatatg ccgtcgtaga tatttctcca aatactaaaa caagaccagt
     9781 tgttacagac gtttcacacg ctactcgtgc tgttggatct tgttttaaaa ttgcatccaa
     9841 gattgcatca ctgatttggt cagcaatttt atctggatgt ccttcagaaa cagattctga
     9901 tgtaaataag tgtctttcta ccattataat gattccccct agttaaattc ggttacaagg
     9961 catctccgtc ctgcggatcc tttcgggaac ttgcaacgaa ccaagtatag cagataaaac
    10021 agttttctgc tcgtattttt tcattttttt gcttaaaaaa tataaaaata ttttacatta
    10081 gaactgtcca aaaaagctta gcacaatcat ccataaagat aactctgcta agctttttaa
    10141 aatcattctt tcagataaaa agaaaaagat actcaaaaag tatcttccat gacccgtacg
    10201 ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc
    10261 caataaaata aatgggccta aatggactcg aaccatcgac ctcacgctta tcaggcgtgc
    10321 gctctaacca gctgagctat aggcccataa aaactaaaag cgggtgacga gaatcgaact
    10381 cgcgacaaca gcttggaagg ctgtggtttt accactaaac tacacccgca taatggcgcg
    10441 ggacagaatc gaactgccga cacatggagc ttcaatccat tgctctacca actgagctac
    10501 cgagccaaac ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg
    10561 ttaaccccta caccacggaa ccaatgtttt tagtttttaa ttgcgggagc aggatttgaa
    10621 cctacgacct tcgggttatg agcccgacga gctaccagac tgctccatcc cgcgataata
    10681 tgaaaaagga ggataaggga ttcgaaccct tgcacggttt tacccgcctg acggttttca
    10741 agaccgttcc cttcagccgg acttgggtaa tcctccatgt aaaactatat taaattaatg
    10801 acccgtacgg gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga
    10861 ccaacgggcc aggtaaaaca aaatattgaa acggagaagg agggatttga accctcgcgc
    10921 cggtttcccg acctacaccc ttagcagggg cgcctcttca gccacttgag tacttcccca
    10981 aaaccaataa gattttgtac taatgggcct aaatggactc gaaccatcga cctcacgctt
    11041 atcaggcgtg cgctctaacc agctgagcta taggcccata aaaaactaaa agcgggtgac
    11101 gagaatcgaa ctcgcgacaa cagcttggaa ggctgtggtt ttaccactaa actacacccg
    11161 catggcggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca ggcatgttaa
    11221 cccctacacc acggaaccag tgtttttagt ttttaattgc gggagcagga tttgaaccta
    11281 cgaccttcgg gttatgagcc cgacgagcta ccagactgct ccatcccgcg ataatatgaa
    11341 aaaggaggat aagggattcg aacccttgca cggttttacc cgcctgacgg ttttcaagac
    11401 cgttcccttc agccggactt gggtaatcct ccatatgaaa cttacaacgc cacaatggac
    11461 cttgtaggac tcgaacctac gaccggacgg ttatgagccg tctgctctaa ccaactgagc
    11521 taaaggtcca ggctcttaaa aatcgcggcg gaggggatcg aacccccgac ctcccgggta
    11581 tgaaccggac gctctagcca gctgagctac accgcgaaaa agatattcat tatttaaatg
    11641 aaatggagcc tagcgggatc gaaccgctga cctcctgcgt gcaaagcagg cgctctccca
    11701 gctgagctaa ggccccgtgt ttaaatttat aattatcggg aagacaggat tcgaacctgc
    11761 gaccccttgg tcccaaacca agtgctctac caagctgagc tacttcccgt ttaattaacg
    11821 cgcccaagag gagtcgaacc cctaaccttt tgatccgtag tcaaacactc tatccagttg
    11881 agctatgggc gcatatttga aatgccgagg accggaatcg aaccggtacg gtgatcactc
    11941 accgcaggat tttaagtcct gtgcgtctgc cagttccgcc accccggcgc gattgctttg
    12001 gcaaagcgga aaacggggtt cgaacccgcg acccccacct tggcaaggtg gtgctctacc
    12061 actgagctat ttccgcgtgt ttaaagatgc cggctaaagg acttgaaccc tcgaccctct
    12121 gattacaaat cagatgctct accaactgag ctaagccggc atgtaaaact taaattatgc
    12181 gggtgaaggg acttgaaccc ccacgccgta aggcgctaga tcctaaatct agtgcgtctg
    12241 ccaattccgc cacacccgca aaaatatgag ccgtacaggg ctcgaacctg tgaccctctg
    12301 attaaaagtc agatgctcta ccaactgagc taacggctca tatggaggtt aacgggatcg
    12361 aaccgctgac cctctgcttg taaggcagat gctctcccag ctgagctaaa cctccatgga
    12421 taactt
//
LOCUS       DAZKOB010000083        12335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000083 DAZKOB010000000
VERSION     DAZKOB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.083"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG8M6_002174"
     CDS             <1..154
                     /locus_tag="ACG8M6_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0457799.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACG8M6_002175"
     CDS             complement(208..486)
                     /locus_tag="ACG8M6_002175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457800.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACG8M6_002176"
     CDS             complement(601..1218)
                     /locus_tag="ACG8M6_002176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457801.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACG8M6_002177"
     CDS             complement(1613..2437)
                     /locus_tag="ACG8M6_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0457802.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACG8M6_002178"
     CDS             2780..3196
                     /locus_tag="ACG8M6_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0457803.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACG8M6_002179"
     CDS             3193..3663
                     /locus_tag="ACG8M6_002179"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0457804.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACG8M6_002180"
     CDS             3677..4450
                     /locus_tag="ACG8M6_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0457805.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACG8M6_002181"
     CDS             4464..5276
                     /locus_tag="ACG8M6_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0457806.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACG8M6_002182"
     CDS             5330..6295
                     /locus_tag="ACG8M6_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0457807.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6431..9154
                     /locus_tag="ACG8M6_002183"
     CDS             6431..9154
                     /locus_tag="ACG8M6_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0457808.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9167..9799
                     /locus_tag="ACG8M6_002184"
     CDS             9167..9799
                     /locus_tag="ACG8M6_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0457809.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10007..10231
                     /locus_tag="ACG8M6_002185"
     CDS             10007..10231
                     /locus_tag="ACG8M6_002185"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0457810.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10333..10566
                     /gene="relB"
                     /locus_tag="ACG8M6_002186"
     CDS             10333..10566
                     /gene="relB"
                     /locus_tag="ACG8M6_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0457811.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10556..10825
                     /locus_tag="ACG8M6_002187"
     CDS             10556..10825
                     /locus_tag="ACG8M6_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0457812.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11004..12305)
                     /locus_tag="ACG8M6_002188"
     CDS             complement(11004..12305)
                     /locus_tag="ACG8M6_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0457813.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4029 a         1998 c         2422 g         3886 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcag atgcagcgat taaatgacta tctttatcaa ctaacattgc ttttgaaatc
     6481 tacgccttca gataattatt cagctcgcag tatttcgcaa gctctacagt tgaatagaag
     6541 tacaatcagt agctacttaa atgaaggtgt tcgtgaaggg cttttcataa aagtaaaaag
     6601 ttacccggtt ttatttttac atagaacagc tcttgaagaa ctacatatca cactgaataa
     6661 ctccgaaata gaatcaattg aaagcttgtt aacaatttct caaaaacccg cattagatca
     6721 agtaattggc tctaaaggaa gtttgaaaga agcaattgat caaattaaaa cggcagtctt
     6781 atatcctgga aaagggttgc ctttattatt gatcggcgct agtggatcag ggaaaacctt
     6841 tctagcgaat aaaatatatg aatatgcagt agaagaaaag gtcattcaca catcggcacc
     6901 tttcatagat tataattgtg cacaatatgt gagcaatcca gagttactat cctctgcttt
     6961 atttggctac acgaaaggag catttaccgg agcaagccag gagcatactg gtttgctaga
     7021 aaaagccgat ggaggagtat tgtttttaga tgaagtgcat cgcttatctg aagaagggca
     7081 agagaaatta tttacattta tggatacggg tgaattctct cctatggggg ataatagtat
     7141 tcggaaaaaa gccgatgttc gattagtctt tgcaacaaca gaaaatatat atacgacttt
     7201 cttaccaact tttcttagga gattgccagt tattgttaat ctacctagat ttcaacaacg
     7261 tccgtcgttt gaacgtctgt cattgattga tgaatttttt gtttctgaga gccaaatttt
     7321 agctaaggaa ctatctgttt ctgatgcatt gattcatttc ttaatgaata gtgattttga
     7381 aggtaacgtg gggaatatta aaaatattat caagtatgct tgcggaaacg catatatcca
     7441 ccagaagagt cagcaaacaa ttcaagttaa attattggat atgcctttag agtacagtta
     7501 taaattcaag gagcaactaa ataaacccag aaagaaaaga gcagatcgaa tctactatcc
     7561 agaagaaaca cgacaagtgc aactagaaag aaacaatcag ctttttgttc aattttttga
     7621 ccaattactt actggattct ctgaagtgat tgaaaataac aaggatatta aactattttt
     7681 agaagaaatg gttaccaagg taacacaagt aatggatacg cttatttttc aagaggatta
     7741 cgaaaaagag cagtctttat acgccattct aaactatcat attcgtcaaa gtattgattt
     7801 tatgcgagaa agctacgggt ttgaacaaga tggtaaccgg gtagtcgctt tggccaacta
     7861 tctatatgtc aaggataatc aagtgatttt tgaaaatagt cttcaagaaa agaaaaccaa
     7921 agatcagctt aacgaatttt ttgctaatgt tcttgaaacc ccttattggt atgcaaaaaa
     7981 aatcttaagc cttttatctg agtgtttaga tcaacctgtg tatgaagagg acattttctt
     8041 tattacgcta tatttttata gtttgaatgt cacgaatgtt aaaacaggta ttaagagtat
     8101 tgtgctagct catggttatt ctacagctag tagtatggcg aatgttgcca accgtatgct
     8161 gaataagaac gtttttcaag cttatgatat gcctttagat attacattag agaaaattga
     8221 agcaaaattg attcaattcc ttggagatta tcgaacagat tcaggcttga ttttattggt
     8281 agatatgggt tcactaaacc aactaggcgg tttattagtg aatcatctaa atggaccact
     8341 gttaatcatt gatcatatca atacaccgtt attattggaa gtcggccaac aagtattaaa
     8401 caataaaagt atcaccgaaa taggagcaga aattgaaaca agtcttactt tcaaaaagca
     8461 gttactactt cccaagaaaa agaaaaagaa agccatcatt acttgttgtt atacagggat
     8521 tggtagtgcg acccagattc aggagatttt aacgaaatgc ttgaaagatg cagttagaga
     8581 attagtaatt attccgtatg attacaaaaa gctgttagaa aacaagaatt ttgagactcc
     8641 ttttcaatta tacgatgtca tcatgattgt aggtacagaa gatcccaaaa ttaatcagat
     8701 tccatatatt ggattagatc gcctgattaa tgggggaagc attggcgaat ttgttcatta
     8761 cttggaaaaa gagctgacta ttgaagtgaa cgatttacaa aaagaattaa ttttcaattt
     8821 ttcaattagt aaaattgtcg aaaatttaac cattttggat ccagataagg tgctatcttt
     8881 tgtacaaaaa gcaattaaag aattatcgaa gttattaaat tttgattttt ctaataatca
     8941 gctgtttttg ttgtaccttc actgtagttg tatgattgaa cgaattgtac gtaaagaaag
     9001 tgtggatgag caagaggaca ttgaagaata tatgcaagca aatggagaaa taatggataa
     9061 aattcatagc gctttttttg agattgaaag ggaatacaca attgaaatac cagatttaga
     9121 gttgcgatta ttgagcgata ttgttcaagg ataggagtga attcgaatga caatgggagt
     9181 aatttttgat atggatgggg tactcataga tagcgagaca ttttattttg agagaagaat
     9241 gaacttcttt caagaactgg agatagatcc aggatcaaac cagattgatg attatgtggg
     9301 gaaaacagaa gctgcaatat gggaaacatt agttcctgag gacaaatcat taagagagga
     9361 actttatccg aaatatgtag attatcgaaa aacgcatcca attgatttcg ctaaagcttt
     9421 acgaaaagaa gtgaaaccgt tacttgactt cctcaagaag cgggatatac cattggcact
     9481 ggcatcttct tctccagctg tcgagataga gagtatgctc attcaatgtg aacttactga
     9541 atatttttcc tttgttataa gtggtgagtc attaaaacaa agcaaaccac atcccgaaat
     9601 atatttaaaa gcgaaacagc aattagactg tgacttcttt attgcagtag aggattcaac
     9661 gattggtatt aaatcagcaa aggcagcaaa tatctatacg gcggcactaa aacaaccaat
     9721 gcaaatcgat cagagtgaag cagacataca aattgaaagt ctacaagaac tggaacaaat
     9781 tgttttagaa aaaggttagt gaattaatgg aaataaggct atatatctgt gctagctatt
     9841 ttgactaacc aaaaaaactt gctataattc aggtgtagta aaaggtgctg gtaacacctt
     9901 ttacgaaaat gcttcggcag tgataatggg attggctttt taagtttttt attttgttcg
     9961 caatccctaa ggtcatgtga atgatcctag ggattgctag aatattttgc aacagaacca
    10021 aaatacgaat agttatcaag cagccatatt gactgcggtc aatttaggag gggatactga
    10081 tactactgct gccgtaactg gtgctcttgc gggtattctt tacgggggtg aagtgatccc
    10141 tgaaaattgg agaaaatcgc ttcaaaatga ccagcttctt aaaacgatca gtcagagatt
    10201 ttgcgatacc gtgctatctc aaagaatttg aataatggat atgatgcaaa tcttgtttgt
    10261 actaacgttg tattgacaaa aaggatttta tttagtacaa tgttagtaca aatgatgaac
    10321 ggaggtagaa tcatgtctca atcaaccatt acatttagga ttcccgacga tgaaaaagaa
    10381 ctggtattag aatatgcaaa agcacacaat tcttcattga ccgaacttta tcgaaatgct
    10441 gtactggata aaatcgaaga tgaaatcgat ctacggactt tacggagtgc aattgaagtt
    10501 tccaaagaga aaaaagagag tggtatttct caagaggaaa tggagaaact gctaaatgaa
    10561 ttataaagtc atctacatgc cgcaagccca gaagcaatta aaaaaaatgg acagaaacca
    10621 agctaaaata attgtcgcct ggatcagaaa gaatttagaa aacacgactg acccgcgtca
    10681 gcacggaaaa ggtttgactg gaaataagag tggggaatgg cgctatcgaa ttggaaacta
    10741 tcgaatattg gctaatattt gtgattctga aattgaaatc cagatattct caattggaca
    10801 tagaagtaca atatataaaa gataaccgtt tttggaacga agtagagttt cgaaagtcaa
    10861 tgtataatct tttgaatttt gcataatacc acaggtagaa actacccgat actatcggtt
    10921 ggtttctgct tttttatggc tctttgtcaa ataggactga tagagtgaga taagaacatc
    10981 taaggtagaa ttccttttct actttagatg ttttttgatt cataggtaat cagattattt
    11041 agtaagaaaa ttctggtctt cattttccga aagcttcgaa agccataagc gactcgtttc
    11101 aatgctttga tgtgggtatg cagattttct attttggcat ttgaataagg caattcgagg
    11161 gctaaacgaa tgccggattc atagtttata aaagcttttt ttatagaatg cagttctttt
    11221 ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag tgtccgcctc tttgtcgtga
    11281 aacgcttgaa gaatttcttg atagtaaaaa taggcgattc gtaattcgtc agagatcgaa
    11341 agcagacgat ccaccacttc actttcggtg agatacgccc aaccaaaatt gcgccatttg
    11401 gtccatttgt cataaacaag cttgtcctgt cgtttgatca gaagccgaga caacgccttt
    11461 aattgtcgat aatatttggc ttgtgttgga tcgtttcgat caaagctttt catgattcgt
    11521 acgcgaatat cctcaaattt acgaatgaga tgtttgacaa tgtgaaaacg atccacaacg
    11581 agttttgcct ttggaaaaca ttcctttatc agagaggcat aagaaacatt catgtccgtc
    11641 acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt gcataaaata agaaacgaga
    11701 gcctttttct tccgtgaagg aagaatatcc agtaattttc cagtatcgcc atccatacaa
    11761 gaaaaggagt agggaccgac ttgatttttc agtgaacgaa attcatccat taataaacaa
    11821 cgcggtaacc aatttctttt tacttttgtc atggggatca gagaagcgag tgtccgagaa
    11881 acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg tcattgcttg tttttcagtt
    11941 agctcttcta aaattttata gcgcacttgt ttagcgataa agcagtttct atcgacaatg
    12001 taggtattcg ccgtaaaata ggtgtggcag ccacgacagt ggtagcgttg tttcgctaaa
    12061 gcaagataag tcagttggtt cgcacattta ttaagttgaa tgagcgattt tttctttccg
    12121 ttcttaacga tcatgccttc atgattcatc tgtttacaca aaggacagcg acgaatttct
    12181 ttacttaacg tcgcagaaac aacaagactc ttgatattct taatgctctt cgattccaca
    12241 tttgtgatga aaatgttttt atctttgatt ccaagtagta gtttgatatg atgaagatca
    12301 ggcatacaga acgctccttt gattgtgttg tggtg
//
LOCUS       DAZKOB010000084        12210 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000084 DAZKOB010000000
VERSION     DAZKOB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.084"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..650)
                     /gene="frr"
                     /locus_tag="ACG8M6_002189"
     CDS             complement(93..650)
                     /gene="frr"
                     /locus_tag="ACG8M6_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0457814.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(653..1375)
                     /gene="pyrH"
                     /locus_tag="ACG8M6_002190"
     CDS             complement(653..1375)
                     /gene="pyrH"
                     /locus_tag="ACG8M6_002190"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0457815.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1511..2392)
                     /gene="tsf"
                     /locus_tag="ACG8M6_002191"
     CDS             complement(1511..2392)
                     /gene="tsf"
                     /locus_tag="ACG8M6_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0457816.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="ACG8M6_002192"
     CDS             complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="ACG8M6_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0457817.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3632..4111
                     /locus_tag="ACG8M6_002193"
     CDS             3632..4111
                     /locus_tag="ACG8M6_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0457818.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4328..5419
                     /gene="phnW"
                     /locus_tag="ACG8M6_002194"
     CDS             4328..5419
                     /gene="phnW"
                     /locus_tag="ACG8M6_002194"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0457819.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5412..5540
                     /locus_tag="ACG8M6_002195"
     CDS             5412..5540
                     /locus_tag="ACG8M6_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0457820.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5544..6089
                     /locus_tag="ACG8M6_002196"
     CDS             5544..6089
                     /locus_tag="ACG8M6_002196"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0457821.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6543..8234)
                     /gene="argS"
                     /locus_tag="ACG8M6_002197"
     CDS             complement(6543..8234)
                     /gene="argS"
                     /locus_tag="ACG8M6_002197"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0457822.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="ACG8M6_002198"
     CDS             complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="ACG8M6_002198"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0457823.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9716..10735)
                     /gene="argF"
                     /locus_tag="ACG8M6_002199"
     CDS             complement(9716..10735)
                     /gene="argF"
                     /locus_tag="ACG8M6_002199"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0457824.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="ACG8M6_002200"
     CDS             complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="ACG8M6_002200"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0457825.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3345 a         2529 c         2203 g         4133 t
ORIGIN      
        1 ataatttttt ctttttttaa tttttaaata aaagaaatat aaataaaaac agaggaagcg
       61 gaatgatccg cccctctgtt tttatgattt tattaaactt ccaataattc tttttcttta
      121 tcagcagcga tcttatctac ttctttgatg ctgttatctg tcaatgtttg tacgtctttt
      181 tctaatccgc gcaaatcatc ttcggtaata tcaccatttt tttgttgttt tttatattca
      241 tcgatcgcat cacgacggat gttacgaatc gcgatttttg cattttctgc ttcttttttc
      301 acatctttag ctaattcttt tctgcgttct tctgtcagtt gcgggataac taagcgaatg
      361 acatttccat cattggttgg actgattccg atatcacttg ccataatagc tttttcaaca
      421 tcttgaagaa tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg
      481 attgatgcca tttggttcaa tggtgttggc gcaccgtagt agttcacagt aatacggtcc
      541 aaaaggctag cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg aagattttgt
      601 gctgcttttt tcattttttc ttttgcttct gtcataattg catctgccat ttttatttcc
      661 cccttacagt tgttccgata ttttcgccta agattgcacg gcgaatattt ccgtgttcat
      721 ttaaattgaa gacaagcagt ggaatatcat tatccatgct tagtgaactt gcagttgaat
      781 ccatgacttg taatcctttg gcaataactt ccagatgagt cagttcttca aattttactg
      841 catttgcatc tactttcgga tcagcagaat aaacaccgtc tacattgttt ttagccatca
      901 ggataacatc tgcgccgatt tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt
      961 acgggttgcc ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa tgacgttctg
     1021 ctttacggcg aatatatggc tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg
     1081 tcggtacacc cacgttttcc aacgtatcct gtaaagctaa ggcattcata actgtagcta
     1141 acatacccat gtaatcagct tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc
     1201 gccagatatt tccgccgccc acaacgattg ccatttcaac gcccagctcg tgaacttctt
     1261 tgatttcttg gatgatttct ttgattactg gtggtttgat tccaaatcct gcgtccccag
     1321 ctaaagcttc tccactcaat tttaagacta cacgctgata tttcggtgtg accatgttca
     1381 ttcctccaat attttctatt gccattttac catacttaga tgatttcgac actctaagac
     1441 tggtttttct tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta
     1501 ttcaacagaa ttattttttc acttggctca taacttcttc tacaaagtta tcttcgcgtt
     1561 tttcgatacc ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg
     1621 caacgtattt ttcaacagtc atatcaggat ctttaacgaa tggttggtcc accaaagcga
     1681 tttctgcttt gaatttcttc aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt
     1741 tgccttcgtt caatgcttgt tctgtaagaa cagttttttc atgttctaat tcagcttctg
     1801 gaatttgtga ttcgtttaca taacgaggat tgatagctgc tacgtgcata gctacgtctc
     1861 ttgcaactgt ttcgtcagtt gttccatcta gaacagtcaa tacggcaata cgtccgccca
     1921 tatgcaagta tccaccaaat gcagcattgt cgtctttttc tacaacttca aagcgacgga
     1981 agctgatttt ttctccgata acttgagttg cttcgatcaa gtcagattca attgtacctt
     2041 tatctgtttt gattttcatt gcagcttcca tgtctgctgg tttgttttca gcaacaagtt
     2101 ctgcaatttc ttttactaaa tcttggaaca tttcgttttt agaaacaaag tctgtttctg
     2161 agtttacttc aacgattgct gcagtgttac ctttcacagc aactgaagcc aatccttcag
     2221 ctgcaatgcg gtcatttttc ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa
     2281 ctgctttttc catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc atcattccta
     2341 cgccagtcat ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc atgtttaact
     2401 cctcctgtgt gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc
     2461 cagcctttga aacagctcca tcattcaaaa ttattctgct gaagaattgt cgccttcaac
     2521 aacatctacg atttcttcga ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac
     2581 tacttgatct tcaccttggt ttccttcgat gaaagcatca gccatttttg aagtaatcaa
     2641 tttaacggca cgaattgcat cgtcgtttga agggattact acgtcgattt catctggatc
     2701 gcagtttgta tcaaccatag ctacgattgg gatatttaat ttgtgtgctt cttgaacagc
     2761 aatacgttct ttacgtgggt caacgatgta cattacatct gggattcttg gcatatcagc
     2821 gataccgcct aagaattttt caagacgttc acgttctttg ttcaaaccag caacttcttt
     2881 tttaggtaga acttcaaaag ttccatcttc ttccattgca ttgatttgtt tcaaacggct
     2941 gatacgtttt tggatagtat cccagtttgt caatgttccg cctaaccaac gatggttaac
     3001 gaagtattgt cctgcacgag ttgcttcttc tttgattgct tcttgtgctt gtttttttgt
     3061 acctacgaat aatgctacgc cgccttcttc agcaacgctc ttcatgtaat cataagctgc
     3121 atctactaat ttaactgttt tttgcaagtc aatgatgtag attccgtttc tttctgtgaa
     3181 gatatatttc ttcatttttg gattccagcg acgagtttgg tgaccaaagt gtacgccggc
     3241 ttctagtaat tgtttcatag aaattactgc catgtttgtt tcctccgatt tggttttatt
     3301 tttcctcttc ttatcttcta ccttgccggc aaactttttc aagcacctac cgacaatcca
     3361 ataagaatgt ggatttgctg atgcataaag caccctcagc tattatacag acaaaagatc
     3421 cgtatcgcaa gagcaaatat acttttctgc tcaacattca atagtttacc tgaaagataa
     3481 aaagaattca agaaaaacag tagatttctc tttgtttctt tattatcctt tttccataaa
     3541 ataattttag gtataaaaga cgaatattat tgggcaatcc ttcttttttt tgttatgata
     3601 catgtgaaat ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat
     3661 taaacaagtc attgaagaat tcccgatccg tacgcaagaa gaactattaa acaaacttgg
     3721 gcattatggc gttaccgcca cgcaggcaac gatttctcga gatatccgtg atctaaaaat
     3781 cgtgaaggca cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac
     3841 tcctgagact aggactgagg aagaggatcg gctggttcaa atgattgaag acattgtatt
     3901 gaaagtcgaa cgagtccact ttttgacgat cgtcaatacc ttgccagaca atgctcattt
     3961 atttgcatct gttttagatg agatcaaacc tccctctatt gtcagtacaa ttgctggatt
     4021 cgatacgatc atggtcatct cggaaaatga ggaggacgca aaacgagtag aagaatttct
     4081 tcaccatcca actgaagtat cactttttta atcagagcac gtattcctaa tatggaatgc
     4141 gtgttttttt attttcaata ttttccttat tgaataaacc tttacactct ttacaaaaat
     4201 acatcatttt ttattcaaat ttttcaacac cttgattgaa tcacaataat catcatttat
     4261 attggatgtt aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg
     4321 aaatcatatg tataaattat taacttctgg accattgacg acttctgaaa cagtgaaaaa
     4381 tgctatgctg atcgatcatt gtacttggga tcaagaatac aaagaaatta cacaatggat
     4441 aagaaaaaaa ttattggatc ttgctcaagt atcggaagga atatacacaa cggtcttaat
     4501 gcaaggtagc agctccttcg gggtagaatc ggtattaagt tctgtgatca ggccagaaga
     4561 tactgtactg atttgttcaa acggcgcata tagacaacga atgatcaaca tgtgcgaatg
     4621 tctgaaactg aactataccg tttatgaggt atcggaacat caaatcctcc aagctcatgt
     4681 gattacacag ctgattgaag agaatccgtc tatctccgca ctagcaatgg tccatagcga
     4741 gactacttcc ggtattttga ataatttgga agagattgct gctgtcacac aagaaagaga
     4801 tcttttgttt atagtggacg cgatgtcaag ctttggtggg ataccgattt gtgtagccga
     4861 actagggatt gattttttga tttccagctc aaacaaatgt attcaaggtg ttccaggctt
     4921 ttcttttgta cttgctaata aagcggaact ggaaaaacga aaaaatgtta gccgaagttt
     4981 gtcacttgat ctatatgacc agtaccgcgt gatggaaaac gagaatggca aatggcgttt
     5041 cacttcccct acccatacgg ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga
     5101 aggaggaatc gaaactcgat attcacgtta tctcgccaat aatcatgtgc tacgacaaaa
     5161 aatgacagag atggggttcc agcctttcat tcaagaatat caggggccat tcatcacctc
     5221 ttttctttac cctaaggact ggcgatttga ttttaatcat ttttatcagt atttaaagca
     5281 aaatggattt gtgatctatc ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa
     5341 tattggcgag atctatccgg aagatatttt ggaactgaca gcaattatca aagactataa
     5401 ggagaacgtt aatgattaaa actgtaattt ttgattgggc aggaacaact gtggactttg
     5461 gctgtatggc tcctgtccac gcttttagaa atgcttttct agaaaaagga acccaactga
     5521 ctgacaaaga aatccgctaa ccgatgggaa aattaaaatg ggatcatatc caacagcttc
     5581 tttccctccc ttctgtcaaa aaacaatgga tagctattta tggacaacta cctcaggaaa
     5641 aagaggtgga ggaattgtat caacaatttg aacattatct gtttaaagat ttggtcaagc
     5701 attctacctt gaaacccgac acattggaaa cgatcgaacg gttgagaaac gctgggatca
     5761 atattggttc cacgaatgga tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa
     5821 aaaaagaaag gtattcacca gacttcatgg caacttttga agacgtcgaa gggtatggtc
     5881 gaccgtttcc ttatatgatc cataaaaaca tgcagtactt caaaaacaag tcggttgatg
     5941 aagtaatcaa agtcggagat acggtctcag atatccagga agggaaaaat gccggagtgt
     6001 tgacagtagg tgtaattgaa ggtagttcgc taatggggat ttcttatgat gagtaccaaa
     6061 aattagagat ggggaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc
     6121 cggcgctgat atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga
     6181 caagaatcca gaatgacttt tatacaaaat aagaggttgt gaaaaagctg tcctgtatca
     6241 agtaataaaa acagccaagc aaaaatagct cctcatattt ttcgtttggt taggcttatt
     6301 accgcagatg caagcttttg aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg
     6361 acttttgtca cagtcccctt atttgcaaac aaacagtgtt taagagacaa cctctccact
     6421 aaacggtgtt cattcagctg actctcggta tttgctcaaa aaacgataaa atttgaaaag
     6481 ctattttctc ttttgtttct ctaatactcg gatcagaacg ctgatttcac attctctttt
     6541 tcttacatct tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt caataataca
     6601 gtcactgcat aaactagagc taagcgtgct tctttttgtt catcatcagc taaaatcttc
     6661 gtatgagcat agtatttgtt gaatgcttgt gctaatttga ttgcatgttt cgcaatcact
     6721 gacggttcgt atttttcccc agcagacaag accgtttcag ggtatttttg aactaattta
     6781 accacttccc agctggtatc gtcattcaat gcatagtctg ctgtttctga agggacaaat
     6841 cctgcttttt ccaacaggct gactgcacga gcatgtgtat attgcacata aggtccagtt
     6901 tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt
     6961 ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt tagctacagc atctttgttt
     7021 tccaaatcag ggtttttctc gctgatttgt tctttagcag attggattgc ttcatttaat
     7081 acttcttcta ataagacgat ctttccttta cgagttgata attttttacc accttgcgtg
     7141 atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt caaagcccat ttccttcaag
     7201 acagctttca actgtttaaa atgatagctt tgttcattcc ctactacata tagagattgt
     7261 ttgaagtcgt aagtacgttt acggtacaat gcagcagcta agtcacgtgt aatgtatagt
     7321 gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt cataagcaga taggtcaacg
     7381 atctctgcac ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt gacgatttca
     7441 tccatcttgt cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga
     7501 tcatagattt tattgaattc tttcatcgat tcatcacgga accattgcca taattgaata
     7561 gcctctttat cgccttcttc cagacgtttg aaccaagcac gagcttcttc atttaattct
     7621 ggttctgttt cagctacttc atggaattgg acgtataaac gaagcaattc attgattggt
     7681 tctgctttaa cagcttcttc tgtaccccat tttttgtaag cgacgatcag ttttccaaac
     7741 tgtgttcccc agtcacctaa atggttgatg cggattggct gataaccgat tttttccata
     7801 atgaaaccga tcgaattacc gataactgtc gaacgcaaat gtcccattga gattggtttg
     7861 gcaatattag gagaagacat gtcgattggt actgttcctt gattcccgat gttgctatcc
     7921 ccataatgtt ctttttcttt cactactgtt tgcaaaactt ttttgctgat aagttcttta
     7981 ttcatgaaaa agttcaagta aggtccgaca acttcgattt tttcaaagtt ggcagaatcg
     8041 attttttctg ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat
     8101 gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt ttggattttc tagtaattgc
     8161 tcgacttgtt ccagtgttaa atcatctttg acagcgtcat agacagcttt tgcgacaaga
     8221 tctttattat tcatctttat tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat
     8281 ctctagcgac ggcatactta gtatgccatc actagagacg agatttcccg cggtaccact
     8341 ccaattgtcc taaaaaagga ccagctccat ctgatatcgg tagacgaccg tctgtttaaa
     8401 acagaattct ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac cacttccgaa
     8461 ttcgctagcc aagaatgata caagtactct cttgttcaac aaatcttagt cttattaaac
     8521 tttcttgaat tatatcagaa aaaaaaacga aaggctagtc gtttcataaa aaaaaaatcc
     8581 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     8641 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     8701 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     8761 tagctgcttc tactttcgga agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt
     8821 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     8881 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     8941 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     9001 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     9061 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     9121 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     9181 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     9241 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     9301 ccttcagtac ttcgcccatg gcgttttgta accagtaacc gatagatcct tctgtcattg
     9361 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     9421 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     9481 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     9541 ttgcatccgt agacaagatg gcattgcccc ctaaagcgac aactactttt cgtttggcca
     9601 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     9661 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     9721 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     9781 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     9841 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     9901 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     9961 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
    10021 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
    10081 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    10141 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    10201 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    10261 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    10321 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    10381 gttcctctac cattttttga ctgaatccac ggaattcgat tccgtcaaac atacgtccta
    10441 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    10501 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    10561 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    10621 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    10681 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    10741 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    10801 agtaagactg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    10861 tgcagcgtgg accaccacga cctcgtgaca attcacttga attgatttct aacactttga
    10921 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    10981 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    11041 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    11101 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    11161 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    11221 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgttcgga attttgattg
    11281 ccaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    11341 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    11401 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    11461 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    11521 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    11581 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    11641 catatagact gctagaacgt acttcgatat cttttgtacg aaccccagcc atgattttat
    11701 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    11761 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    11821 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    11881 atgtcttcgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    11941 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccgtttca
    12001 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    12061 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    12121 attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa
    12181 acgtttttct atttttaagc gtaatttatt
//
LOCUS       DAZKOB010000085        12192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000085 DAZKOB010000000
VERSION     DAZKOB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.085"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(154..318)
                     /locus_tag="ACG8M6_002201"
     CDS             complement(154..318)
                     /locus_tag="ACG8M6_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457826.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            613..1941
                     /locus_tag="ACG8M6_002202"
     CDS             613..1941
                     /locus_tag="ACG8M6_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0457827.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2143..2976
                     /locus_tag="ACG8M6_002203"
     CDS             2143..2976
                     /locus_tag="ACG8M6_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0457828.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2992..3729
                     /locus_tag="ACG8M6_002204"
     CDS             2992..3729
                     /locus_tag="ACG8M6_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0457829.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3884..4852)
                     /locus_tag="ACG8M6_002205"
     CDS             complement(3884..4852)
                     /locus_tag="ACG8M6_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0457830.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5084..5917
                     /locus_tag="ACG8M6_002206"
     CDS             5084..5917
                     /locus_tag="ACG8M6_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0457831.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5951..6790
                     /locus_tag="ACG8M6_002207"
     CDS             5951..6790
                     /locus_tag="ACG8M6_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0457832.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6816..7376
                     /locus_tag="ACG8M6_002208"
     CDS             6816..7376
                     /locus_tag="ACG8M6_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0457833.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7575..9296
                     /locus_tag="ACG8M6_002209"
     CDS             7575..9296
                     /locus_tag="ACG8M6_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0457834.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9461..10603
                     /locus_tag="ACG8M6_002210"
     CDS             9461..10603
                     /locus_tag="ACG8M6_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0457835.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10619..11830
                     /gene="thiI"
                     /locus_tag="ACG8M6_002211"
     CDS             10619..11830
                     /gene="thiI"
                     /locus_tag="ACG8M6_002211"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0457836.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4154 a         2047 c         2532 g         3459 t
ORIGIN      
        1 agtcctactg ccggagtaaa taaagcttat gcaaagaaaa agaaatccaa tcacttgggt
       61 ttctttttct tttattatta tgatgaatca agctttctgt ttttgtcacg tatgttgtaa
      121 tctttacgaa agagtagagt tggctactca taatcaacca tacttcccat catcgctaaa
      181 tacgaaataa atcagcattc ctacaccaat caaagcacaa gctgcaccaa gaaaagggat
      241 gacattccca aagttatctg tattcataag tatgccacca cccacaaaca aaaagccgcc
      301 agcaatcagg tttttcattt tattcatctc ctttcttacg ttatagtatt tccttctttc
      361 aatgagtatt ttgaaagcta tcttattgta tgatgttctt ctaggcctct tctttattat
      421 acaggccatt ttcgttatct catagaaata ttccttttta tctgaaaact attgtatacc
      481 gtatgacatt tttcttcatg aagttgtaag tatattctca ttcaaatgaa aatttcatca
      541 tagaagagat tgtatttcta aattcttaag agtaaaatac gaagtataac ttggatttgg
      601 aaggatgatt ggatgaaagc tattacgcct gatatgacaa caaaatttaa caaacagttt
      661 caagcaaata tcaaacagca ggcattgcag cgggcagttg tgaagaacgg aattactgct
      721 tctgctgaaa atgtttcagc acatgtggag aatacaccgg tattttctat agatttggct
      781 acggggaaag tggctaatca aatgcaatcg gggcgttgct ggatgtttgc tgcactgaac
      841 acattccgtc ataagatgct gaatcaattc catttaaaag agtttgaatt atcacaaaat
      901 tacacttttt tctgggataa atatgaaaag gcaaattatt tctatgaaaa catcattgca
      961 acaggaaaag aggcaatcga tagccggaaa gtagctttct tactcgctac tcctcaacaa
     1021 gatggcggcc aatgggatat gatcgtttca ttattccaaa aatatggcgt tgtgcctaaa
     1081 tcggtgatgc cggaaagtgc gaatagttca aattctcgag atttgaataa ctacttgaac
     1141 aaattactgc gtaaagatgc agtagtgtta cggaaaatgg ttgctcaagg agaaacatta
     1201 gacgctattg aagaaaagaa agaagaaatg ctagaaagca tttataactt cttagctatc
     1261 tcattaggaa cacctccaaa agaattcgat tttgagtacc gcgatgaaga aaagaactat
     1321 catcttgacc gaggactgac accacaaatt ttttatgata aatacatcgg tgtaaaatta
     1381 gatgattatg ttagcgtgat caatgcacca acaaaagaca aaccattcaa tcgttcttat
     1441 acagtagaga tgctaggaaa tgtcgttggc gggaaagaag tcaaatatct gaatgttgat
     1501 atggaaactt tcaaaaaatt agcgattgct cagcttgaag aaggagaatc tgtttggttt
     1561 ggttgtgatg taggtcaatc ttctactaga actagtggca tcatggcctt agatgcttat
     1621 tcaatggatg atctatttga tacggatttt acaatgacta aagctgaacg tttagatttt
     1681 ggagaaagtc tgatgaccca tgcgatggta ttgaccggtg ttgacttagt agacgggcaa
     1741 tcaacaaaat ggaaagtcga aaatagctgg ggcgaaaaag ttggaaagaa tggcttcttt
     1801 gttatgagtg atgcctggat ggacgaatat acgtaccaaa tcgttgtaag aaaagattta
     1861 ttgacgaaag agcaattaaa tgcgttcaat gaagaaccaa ttgttctatc tccgtgggat
     1921 ccaatgggtg ctttagcata gacgagtatt tcaatgaaga gtatgtagta aagataaaaa
     1981 gagctggctt aagccagctc tttttatctt taaagtccat tccctgtttt ttatggtccc
     2041 tgtgctataa ttctaaataa aggataagga ttggaaataa tccttttatc attaaaaata
     2101 tgatgacgaa gtagaataaa attgggagta ggggggaaag tcatggatat ccaaactgtt
     2161 ttatctgatc tgaaattaaa tggtaaagtg attccagttg ttggtggcga tgttaatcag
     2221 acctatcgta taaaaaccga acatcgtgct tattttttaa aaatccatcc gaatgtaaaa
     2281 aaaggatttt ttgaagcaga agtagacgga ttaaaagaac tgtcagcatt cgtacgtgta
     2341 ccggatacgt atatgctagg tgaaacaagt gaaggcgctt atttgttaat ggaatggatc
     2401 gagccgggaa aaggggatca acgagatctt gctgcagctt tagctaatct ccatcagcaa
     2461 acagcgccac aatttggttt tcgtaaagat aactatttgg gtacacttgt tcagaaaaat
     2521 tcttttgaag aagattggtg gacttttttc tttaaagatc ggcttgaatc ccagatttct
     2581 ttggcagaag aaacgaatcg atggaatgta cagcgtcaag aaaagtatct gcgatttaaa
     2641 gaacgtgtat tgaaatcggt tgaaccaaaa aagatcactc cacgcctctt gcacggagac
     2701 ctgtggagtg gtaatgtgtt ctttgaccag caagggcatc cagtttttgt cgacccagct
     2761 gtttcttatg ggaatcggga acaagatatt gcaatgagcc agttattcgg tggtttccga
     2821 ccggaatttt tagatgctta tcaaactata tttccattag aaaagggatg gaaagaccga
     2881 ctgccgattt accagttata ttatcttttg gcacatttga atatgtttgg agaaagttac
     2941 ggttcccaag tagatcaatt actagaaaat ttttaagagg agggcgtgtt tttggcaaaa
     3001 aaatatacca aaaagcatga agtttcatat tatgagtgtg acatcaacca aacgatgaca
     3061 tttccttcga tgttaggagt agttatcaaa acgtctgaag atcaaagcga ccagttggga
     3121 agaggcagtg aattcgtcaa tagctttgga cttacttggg tcatcacgaa ttattccatg
     3181 gagattaatc gtctccctag agtcggtgaa gtaatctcag taacgactca agccatggag
     3241 tacaataagt ttttctgtta tcggaacttc tggatccatg atgaagatgg gaaagaatta
     3301 gtcaaaatag aatctgtttt cgtattgatg gattttgtga atcgaaaaat gagcagtgtt
     3361 aatgaagaga tcattgctcc ttttgagagt gagaagatca agaaaatcaa gcgtcaggaa
     3421 aaaattgaaa agattgattc gggcttcatg ttgccatatc gtgttcgttt ttatgatatc
     3481 gatagtaatc agcatgttaa taatgcgatg tattttaatt ggatcattga cgtattagga
     3541 tatgactttt tgactacgca tgttccagaa aaagtaatta ttcgatttga taaagaagta
     3601 gagtacggga atgagatcga aagccatttt gaacaaatcc ctatcgaaaa tggtgtcaaa
     3661 acaaaacatg aaatccgaat gcaagaacag ctgtattgtg aagcaaatat tatatggaaa
     3721 aataaataaa aaagaggttg tgacagaagg gggcaacttc aagaaataag aagaaattta
     3781 cgaaaattgt ttctcaaatt tttgtaaatt ttcgacttag ttttcgaaga agctattttt
     3841 gtcacagcct ctctttttaa gtttagctat tagataatga tgtttagttt tggataaacg
     3901 aaatcaattc ttcacccgac aaaccatagt ttacagcaat caatagtttt aaacgtgctt
     3961 tttgactatt aatactgtta cagaaaataa tccccatttt ttctagttct tttcccccgc
     4021 cttcataatc ataaacaggc tctgcgattc cattgaaaca acgagaaaca agtactacag
     4081 ggattttttg gacaagaagc ttttccaaag cagctaaggc ttgtggaggg agattccccg
     4141 cacctaaagc ttcgattacc aaaccgtcta ctttcgtatg ggcgatggct tccaataagt
     4201 ccccttgcat cccagcatat gctttaacga tcgggatagt cccatctaca gcggaaatgt
     4261 ccagtcgctt tgtttctaaa agttcctgca agaataaaat tcgattctta gttactaagc
     4321 cgatgggacc caatgtaggt gttcggaaag tagcaacatt tgtggtatgg gtctttgtca
     4381 cgtagcgtgc agaatggatc tcatcgttca tgaccaccaa aaccccctta tctagcgctt
     4441 cttcacaact tgcgacacga atcgcacttt caaaattata aaggccgtca cttccaagtt
     4501 cattacttga acgcattgcc ccagtcaaaa caattggtaa tttgttgcca atcgttgtat
     4561 caagaaaaaa agcagtttct tctaatgtat ctgtaccgtg tgtgatcacc actccggata
     4621 tacctgatgc ataagcggac aagatacgtt cttttaatac caacatgtga gaaggcatga
     4681 tgtgtggtga gggaagattg aagatatctt caactaataa ttctacatgc ttggggattg
     4741 tgatttctgc atctaataat gggtttgcca catcaggact aacaccgccc gtgagcatgt
     4801 tttctttcat ggcgatcgtt ccgccggtgt gtagtacaag tatttttttc attttacaga
     4861 cttccttacg aataattttg catagccgtt aaatcttatt gtatactata taaaagagtt
     4921 ttgctatcag tgaagaataa cgaatagcct aacttatcat aaaaaagctg accttattaa
     4981 aatggaaaag actcaacctt gacctcctaa tacaatggag tatctggttg agtttctttt
     5041 acaagaaagc agccaaggaa atcaatcacg aaggtggccg gaaatgatca gaatcggatt
     5101 aacatcgttt aaagaacatg acaaattaac tggtaaaaag aataacactc tttatgaata
     5161 tgcagggttt ctacccttag tggaattaga tactgcctat tatggaattc cccgaaagtc
     5221 ctcagttgag aattggataa gtgaagtacc tgaatcattt cgctttgtag tcaaagtcta
     5281 tcaaggaatc agcggtcaag gagactggaa gccttattat cagaccgaag aagagatgat
     5341 tgcgcatttt ttagatgtta tgtcgccaat ggtagatgct ggaaagatgt tttgcttttt
     5401 agtccaattc ccctcatcat tcaaatgtac gaaagaaaat gtccagtact tgcgcaaact
     5461 tagaaagtgg tttgagggtt atcctgtttc tatagaattg cgggattatt cttggtacag
     5521 cgatgaattt cttgaacgta caagaaactt tatgtctaga gccgcttttt ctttagccat
     5581 catcgatgag ccacagttgc tgaatacaac gattcctttt gatccatttg tcaccaatcc
     5641 gaactttgct ctttttcgat tccatggtag gaatgcgcaa ggatggcaag caaatgataa
     5701 agagtggcgt aaaaaaagaa cgttatatcg ttattcagaa aaagaactgc aagaactagc
     5761 acatgaagtc aaagtagttg attctaagac aaaagaggta ggtgtgattt ttaataataa
     5821 ttctggtggt gatgcggctg aaaatgcatt agcactgcaa aaaatattaa atatcaagcc
     5881 agatggactc aatccaaccc aaatggatct gttttaaggg cttattttat tcaagaaagg
     5941 ggcgaagtct atgatcaaag ctattttttt tgatattgac ggaacattgg tcaccaatcg
     6001 ttcaaaagcg ttggaatcaa caaaacaagc aattgaatat gctcggaaaa acggcatttt
     6061 atgcggtgta gcaacggggc gcagcccagt aaagatcaaa gaaatcattg atgaattgga
     6121 attagatatg tatgtcgtgt ataatggtca actggttttt actgcagatc gcacgattat
     6181 cgaccaccct tttgaacaaa aagtattgga acatattgtt gaatttgcag atgagaacca
     6241 tcgacaaatc gtttttggtg cgagaaatcg gttagatgga tcaacgacga tgctattagg
     6301 acaatcgatt tttatcaagc gactagtcag ctttttaccg cgtaaattcc cggtacgtct
     6361 gatgaaaaaa atcctccagg tttttagccc tcaccgacaa aaggatcgtt atgaaacgtt
     6421 agagatatta aaagaaccaa tttatcaatg tatattgctc agtccggagt cagaacagaa
     6481 aaagttagaa catcgctttc ccgagtgtac gtttcagcgt tcaaatacgt atactgtaga
     6541 tatcatccca aaaggtggtt caaaattact aggcatccaa gctttcgcaa atgctgtcga
     6601 aattgaaatg gaagaaatta tggcattcgg cgatcattac aatgatatcg agatgctgaa
     6661 aggtgtagga attggagtgg ccatggggaa tgctcagatc gaagtaaaac atgctgcaga
     6721 ctttgtgact caatcaaatg aagaagatgg gatatacgaa ggactgaaac acttcggtct
     6781 gatttattag tcaatgaaag aaggaaaaaa tcagtatgga agcagagaca ccttttcaaa
     6841 aagtagaaac attccacaaa acttttgatc caagaagacc agagataccc acggctttta
     6901 cagaaaatga agctcataca cgtgctgcgt tcaaaataga agaacttgtt gaatttgttg
     6961 cagctacttc acctaataaa gaacaactgc atgaagctgt tatgctgctg cacgaagcat
     7021 tagataaagc agaagaaaaa atacttggta aaacacagtg gggaagtgat ccgctggttg
     7081 aacaagtaga tgcactgaca gatatccttt actttactta tggttctttt tcattactag
     7141 gaatagtgcc tgatcgaata ttcgaaatcg tccatcaggc aaatatgggg aaattatttc
     7201 cagatggaaa acctcattat gaccctgtaa caaataaagt gttgaaacct gaaaactggc
     7261 agatggaata tgctccagaa cctaaaatcg cggcagaaat ccaacgtcag attcaagaag
     7321 cgattgctaa gaagaaatcc gctgatatag gcatgtgcac aaatgaaaaa gagtgatcag
     7381 agtttcacta tttttgttat ctttatacga tggaaaaaca aaagattttc gtttaagtta
     7441 gtgaatgcat gaaaatagta tgctataatc tagaatagat gtcttttgca cgagagacgt
     7501 attatttgga tttatgccaa gcagaacgcc atctgttgca taaaagatgc atactgaaaa
     7561 cggaggaacc ataaatggga aataatgtta tcatcggaat cgtcattgca atcattatag
     7621 tcgccattgt cgtctttttg gttagttact tgatgaagaa aaaaaaccaa gatcgattgg
     7681 atgccttaga aaaacgcaag gaagaattat ttgacttgcc agtgattgaa gaagtcgatg
     7741 atgttaaaaa aatgcatctg gttgggcaaa gccaaaatac ctttcgtgaa tggagtcaaa
     7801 aatggaatga catatctact tcctcgttcg ctgaactaga aagtcaaatt ttcgaagtgg
     7861 aaaacctcaa tgaaagtttt cggttcttaa aaggaaaaaa agcagttgtt gaagcagaag
     7921 ctacaatgga ccaaatggag aaacaagtga cggaaatccg tgaaggtcta aaggaattgc
     7981 gggaaacgga agaacgcaac tcactagctg ttcagcaggc acttgacgtc tatgaagaac
     8041 tgaaagaaaa agtcagagaa gacgagcatg aatacggacc agctcttttt gaattgcaaa
     8101 aacagatcaa agtgatcgaa aaagaattta cgcagtttgt gactttaaac acttctggag
     8161 accctgttga agcccgcgag gttttggata aagcagaaaa acatacgtat gaagtggaag
     8221 ctctgatgaa aaaaattcca ccgctgtatg aggagctcca taccactttc cctgaacaac
     8281 ttgaagaaat cagtgatact tacgagaaat tgatggaaga acaatatgta ttcccagaag
     8341 aagatcttgc tgaagatatc gcaaaagtaa gtcgaagaat tgaaaattca ttagccaatc
     8401 ttgaaaaaac agaagttgaa acggtcgaat ttgaaaaccg tgaaacagct gatttgattg
     8461 attcgttata tgatatctta gaacgggaaa tggaagctca gagatatgta aaaactaacc
     8521 aatcaacgat tgcagagtat atcaaacata caacaaagaa taaccgtcaa ttattgattg
     8581 agctggatca cacatctcaa agctatacat tgaaccacaa tgaaattggc cgtgtacgag
     8641 gtttccaaac cgaagtggaa gaaatggaac gtcaaaatga acagatgatt ccacagatcc
     8701 gtcaacacga aattccttat tcagaagtgc gcacgttcta taaaacagtc ttcaaagtct
     8761 tagaagatat cgaaaagcaa caagtagaga tcgacgactc attacatgaa ttgagaaaag
     8821 gcgaaaaaga agcgcaagaa aagatcgata cattcgaatt caaattgcgc agtttgaaac
     8881 gttttgtaga aaaacaacgt ttgcctggct tgccgaatga ttacctagaa ttcttttttg
     8941 ttgctactga ccgtattgaa gaactaagtg ttgtcttgaa taaaattcga gtaaacatgg
     9001 aagaggtcaa tcgtttagtt gctctatgtg aagaagatct agaattatta gataagaaga
     9061 cacatgattt agtagatgca gcagcattaa cggagcaaat gctccaatat gcaaatcgtt
     9121 atcgtcatac acacgaagaa gtgagagaag caattgataa aagctactat ttattcaata
     9181 aagaatatca ctatcaagaa gcattagacg aaatcggtac agcattagag cgagtagaac
     9241 ctggtgcttt caaacgcatc gaagattttt attttaatca tccagactta gtataaagca
     9301 aaaaagttag taaaccagca agagcaagac ggattattgt cttgttcttg tctttttttg
     9361 tatatttgaa aattgaaaca agtgaaaatt tcacctgctc ttgaaagcac cttggacaac
     9421 cgttataata gaaaagaatt tcaaaaaaga ggggtttctt atgatatatt ttgataacag
     9481 tgcaacaacc ccaatctttc cgcaaagctt agatgcatat gtaaaaacca gtcagcggat
     9541 tattgggaat ccatcaagct tgcatgattt aggcaatcaa gctaaccggt tgttgcagca
     9601 agcaagaaag cagatcgctg atttgatcca tgtagaacca gaagaaatct attttacttc
     9661 aggcggaaca gaaggagata actgggtatt aaaaggaacg atgatcgaaa aaagagaata
     9721 tgggaatcat atgatcattt caggagtcga acatccggcc gtcagcgaga cagcagaaca
     9781 attaaaagaa ttaggaatcg aagtaagtat cgccccagtg gataaacgcg gatttgtacg
     9841 tgtcgacgaa ttgaaagaat taataagaaa agagacagta ttagtctctg ttatggctgt
     9901 gaataatgaa gtaggatcga ttcagcccat ccaggaaatc agtgatcttt tggatgattt
     9961 tcctaaaatt catttccatg tagatgctgt tcaggcgatt ggaaaagtac cattctcgca
    10021 atggttgaca gaaagagttg attttgcaac attttcagct cataaattcc atggcccacg
    10081 gggaactggt ttcatttatt ggaaaaaggg tcgtcgttta gcaccattac tgacaggtgg
    10141 agggcaagaa agaaatcagc gaagtgggac agaaaatgtt ccaggtattg tggctatggt
    10201 taaagcttta cgtttatcat tagagataaa ggaacaaaaa cctgagcaga cacaacgctt
    10261 aaaagtagct ctaatcaatg cgttgaagac gtatgagaaa gtgacgattt tttcagaagg
    10321 aactgactat gcaccgcata ttttatgttt tgctctaaaa ggaattcgcg gcgaagttct
    10381 cgtccatgcc ttagaagaaa aacaaatatt tgtttctact actagtgcct gctcctcacg
    10441 taaacatatg gcaagcagta cattgcacgc aatgcatgta ccaaatgatc tggctacatc
    10501 tgctgttcga atcagcttag atgaaaacaa ttcgcttgca gaagtcgagc aatttatgat
    10561 tattttcaag cagctttata agaaattttc tgcaatcaat taaaagagga gtgttcttgt
    10621 gaaatacagt gaaattatgg tccgttatgg ggaactttcg acaaaaggaa agaatcgaaa
    10681 aagctttatc atgcaactag cacaaaatgt cagacaagct ttgaaagact ttccagaaat
    10741 caaaatccat gctgatcggg accggatgca tcttttattg aatggcgcag acgggcagct
    10801 agttattcca aaactagcaa aaatatttgg gatccaaaat ttctctccaa gtattcgtgt
    10861 agaaaaagat gtcgaagttt tgaaacaagc tgttcaagac atcatgaaag aaatttatac
    10921 tgggaaagaa acatttaaaa ttatcgctaa aagaagcgat caccaatttg aattgacaag
    10981 taatgaattg aatcaaacat taggtaatgc tgtttttgat attttcccgc atatccaagt
    11041 tcaaatgcgt caaccagata ttccattacg tgtagagatt cggcgtgatg gcgcttatct
    11101 atcgtatgaa acgatcaagg gagctggagg attgcccgta ggaacaagcg gaaaaggaat
    11161 gcttatgctt tctgggggga ttgattctcc tgtagcaggg tactttgcca tgaagcgggg
    11221 agtggaaatt gaagctgttc attttgctag tccgccgtat acaagtgaac aagcactgca
    11281 aaaagcaaaa gatctaacag ccaaactagc accttatgtg ggaacgatcc attttatcga
    11341 agtgccattt accgagattc aagaagaaat caaaaagtca gtgccacaag gatattggat
    11401 gacaattact cgtcgaatga tgttgcgttt gaccgatgaa attcgccgta ttcgtcatgg
    11461 tttagtcatt ttgaatggtg aatcattagg tcaagtggct tcacaaactt tgcaaagtat
    11521 ggtggctatc aatgaagtaa cgaatacgcc aatcattcgg cctgtagcga caatggataa
    11581 acttgaaatc attgaagtag cagaacagat cgatacattt aatctttcta tccagccttt
    11641 tgaagattgc tgtacgattt ttgctcctcc acagccaaaa acacgccctc aactggaaaa
    11701 ggtccatcaa tatgaagagc gtttagcaat agatgaaatg atccaacgag cactagctgg
    11761 cttgaagatg gaagagatac aagcagaatc gtctaatgaa aaaactgaag aattttcaga
    11821 attgctataa gaggttatga aaatagtgtt ttgaccagag tattagagaa acaaaacgaa
    11881 aaaatagctc catgtatttt accgtttttg aactaatgtc gaagattagc tgattgaata
    11941 ccatttatca agaagttatg gaaaagccgg ttctttttta ctaactgaaa aataaaattt
    12001 tatttagaga tcaagctaca gttgataaat gatataaaaa agagaatttc ccatgaaata
    12061 gcgaggttgt gacagaagtg ggcagcttca agaaataaga aggaatttac gaaaattgct
    12121 tttcaaattt ttgtgaaatt tcggcttatt tttcgaagaa gttacttctg gaacaccgtg
    12181 tatcagaggt tg
//
LOCUS       DAZKOB010000086        11823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000086 DAZKOB010000000
VERSION     DAZKOB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.086"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            308..577
                     /gene="rpsN"
                     /locus_tag="ACG8M6_002212"
     CDS             308..577
                     /gene="rpsN"
                     /locus_tag="ACG8M6_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0457837.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            592..771
                     /gene="rpmF"
                     /locus_tag="ACG8M6_002213"
     CDS             592..771
                     /gene="rpmF"
                     /locus_tag="ACG8M6_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0457838.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            803..1180
                     /locus_tag="ACG8M6_002214"
     CDS             803..1180
                     /locus_tag="ACG8M6_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457839.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1201..1350
                     /gene="rpmG"
                     /locus_tag="ACG8M6_002215"
     CDS             1201..1350
                     /gene="rpmG"
                     /locus_tag="ACG8M6_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0457840.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1374..2357
                     /locus_tag="ACG8M6_002216"
     CDS             1374..2357
                     /locus_tag="ACG8M6_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0457841.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2387..2509
                     /locus_tag="ACG8M6_002217"
     CDS             2387..2509
                     /locus_tag="ACG8M6_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0457842.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2527..4050
                     /locus_tag="ACG8M6_002218"
     CDS             2527..4050
                     /locus_tag="ACG8M6_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0457843.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4074..4313
                     /locus_tag="ACG8M6_002219"
     CDS             4074..4313
                     /locus_tag="ACG8M6_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457844.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4506..4967)
                     /locus_tag="ACG8M6_002220"
     CDS             complement(4506..4967)
                     /locus_tag="ACG8M6_002220"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0457845.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5162..5380)
                     /locus_tag="ACG8M6_002221"
     CDS             complement(5162..5380)
                     /locus_tag="ACG8M6_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457846.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5498..7114
                     /locus_tag="ACG8M6_002222"
     CDS             5498..7114
                     /locus_tag="ACG8M6_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0457847.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7355..8695)
                     /locus_tag="ACG8M6_002223"
                     /pseudo
     CDS             complement(7355..8695)
                     /locus_tag="ACG8M6_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9133..9279)
                     /locus_tag="ACG8M6_002224"
     CDS             complement(9133..9279)
                     /locus_tag="ACG8M6_002224"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457848.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9318..9512)
                     /locus_tag="ACG8M6_002225"
     CDS             complement(9318..9512)
                     /locus_tag="ACG8M6_002225"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0457849.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9422..9754)
                     /locus_tag="ACG8M6_002226"
     CDS             complement(9422..9754)
                     /locus_tag="ACG8M6_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0457850.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10268..10459)
                     /locus_tag="ACG8M6_002227"
     CDS             complement(10268..10459)
                     /locus_tag="ACG8M6_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457851.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10553..10939)
                     /locus_tag="ACG8M6_002228"
     CDS             complement(10553..10939)
                     /locus_tag="ACG8M6_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0457852.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            11633..>11823
                     /locus_tag="ACG8M6_002229"
     CDS             11633..>11823
                     /locus_tag="ACG8M6_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0457853.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTG"
BASE COUNT         3941 a         1838 c         2258 g         3786 t
ORIGIN      
        1 agttatagtt tctttcatat tgccgtttta agtagaggaa aagctactta aaacggtatt
       61 tgtgtttgat tggactaata aaagtgaatt cttatttttc agagcctaaa ctgcatattc
      121 ttttaaatga tttgattatc tctatgtacc gataaattcg ttttcaaata gttatctatt
      181 catatgagtt ttatttcatg ttatttattg ttgacaagag caactcttgt tggtaaagta
      241 tcagtgtcaa aacgtaacga ttacgatttg aggtgtctaa atcaaaataa aaggagtata
      301 aaggtatatg gcaaaaaagt cgaaaattgc taaagcaaag aaacaaatgg cgatgattga
      361 aaagtatgca gataaacgtc aggaattaaa agccgcagga gatcgcaccg ctctcgctaa
      421 attgccaaga gattcaaatc cgaatcgtct gaggcttcgt gatcagacgg atggtcgtcc
      481 aagagggtat atgcgaaagt tcggtatgtc ccgtattaag tttagagaac ttgcccatca
      541 agggttgatt cctggagtaa aaaaagcaag ttggtaaaag gaggaaatat catggcagta
      601 ccagcaagaa aaacatctaa ggcgaagaaa cggttaagaa gaactcatca aactgtagta
      661 aaaccagaaa tttcatttga cgaagcgaat ggtgattata gacgtagcca tcatgtatct
      721 ttaaaagggt actataatgg aaagaaagta attaaaggag cttcaaaata gtttaatata
      781 taacttttag gagggaatca aaatggaaat tatttaccca cctttagttg aagaaggatt
      841 aaagtattat cttgaaacga cccagcaatc gctagataaa tgcacttttt atcgttcgat
      901 ggtagagaga ggaattatta ctgaaacggg tttaccgacc caacaggcaa ttgaaaatgg
      961 attagtcaaa gattactatg aggaccaagg cttatcattt gatgattttt taagaatcta
     1021 tccaattttt gaagagtatg acgaggagtt atttcaatgt atcgatggtt attgggagat
     1081 acctgttgat atgaaagaaa atttagtttc acaattggaa tctggcgagt taacttttga
     1141 agatgcacaa caaattcaag catatcttga agatcgataa gtattgaagg aggaagattt
     1201 atgcgtcaaa atattatttt ggaatgtgtc gaaactggag aacgtctgta tctcaccagc
     1261 aaaaataaac ggaataaccc tgaacggtta gaattaaaaa agtattcgcc aaaattgcgc
     1321 agacgggcaa tttttaaaga agtgaaatag ctaagtggta aaaaggtgac aaaatgggca
     1381 taccagttac aatcgtttct gggtttttag gggctggaaa aacaacgcta atcaatcaag
     1441 ccttgcaagt ctcaccctac cccagggaag aaattattat aattgaaaat gagtttggtg
     1501 aagttgggat tgatcacaaa ttattagttc atagcgagga acgggtgtta caactaaata
     1561 atggatgtat gtgttgcagt cttcgcagtg atttattagc aatattgatt tctttactgg
     1621 aaacaataga agaacatcaa caaccaattt cacaaatcat cattgaaact acaggtatag
     1681 cagatcccca accaatcatt caaacacttt taacagcgcc tcaactaaga ggaagagtat
     1741 atatcgatag tttattgact gttattgata gtgacagttt tgaacttatt gaaagagaac
     1801 cacaagccct taagcaactt gtcatggcag atcgcatttt tgtgtctaga aaacaaaact
     1861 tgaatttatc tgcactaaaa aaattacaga gagaaatcaa aagtattaac cctttatccg
     1921 atattcgaat gttcacacat agtgaaacga ttcaaaaaaa ggatttttat aatttgaaaa
     1981 aatttaatca tcccttagtt tttgaaagtg attcatcaga aaatcactat aatggcaaat
     2041 actatcatca acaccatcaa gtacatgagt ttaaatcaat tctcttaact tcagatcaag
     2101 acataaaaaa agatttttta ctccaatgga ttgactggct gattttaacc aaccaaggga
     2161 gcctgtatcg ttttaaagga ttgattgctt tacaagagca ggattttata gttgccatgc
     2221 aagaggtaaa cgaacaggtt aattttcaat acaccactcg acaaaaagac caaaccacta
     2281 ttattctaat cggaaaaatg ttggatgaag aaaggatcaa acagagtttt gataaattac
     2341 tggcggaaag caaataatga taagtagtga ttgggggtaa tttctaatgg agaaaacgga
     2401 tatatctagt gcatatcgac gacttaaaag cccgaatatt aaaactagaa aaagggcatt
     2461 aaaaattatc aaagaacata aacgaaataa aatgaagaaa ttagcataat taaggaagga
     2521 aaaagaatga agaaaatcag tgtaggactt attgggatag cagctctagg attattggga
     2581 gcttgctcca gcactaatga tgcaaaagta tcgaatgata aagatggaaa attagaaatt
     2641 gtaacaacat tctatccaat gtatgatttc accaaaaaca tcgtcggcga tgaagcgaac
     2701 gtggatttaa tggttccagc gggatcagaa cctcatgact atgagccctc agctaaggat
     2761 atggcaaaag ctcatgatgc tgatgttttt gtatatcata acgaaaacat ggaatcatgg
     2821 gtaccaaaag ctaaagaaag ttggaagaaa gctgggccta atgtagtaga aggaacaaaa
     2881 gacatgattt tattgccagg tagtgaagaa gaagatcatg atcacggaga agaagatcac
     2941 catcatgaac tagatcctca tacgtgggtt tcacccaaaa tggcaattaa ggaagtttca
     3001 aatatcaagg atcagttagt taaattgtat ccgaaaaaag caaaagtatt tgagacaaat
     3061 gctgaaaagt atttgactaa actgaaacgg ttggatgctg actataccac tagtcttaaa
     3121 gaagcgaaac aaaaaagttt tgtgacgcaa cacgcagcct ttggatattt agctttggat
     3181 tatggattga aacaagttcc tattgctgga ctaagtcctg aagaagagcc atcttctggt
     3241 cgattagctg aattaaaaga gtatgttaaa aaaaataaga tcaattatat ttactttgaa
     3301 aagaatgcta atgataaaat tgcaaaaact ttggccaacg aagctgggat aaaattggaa
     3361 gtactgaatc ctcttgaaag tcttacaaaa gaacaaatgg ataatggtga agattatgtt
     3421 tcagtaatgg aagacaatct aaaagcgtta gaaaaaacaa ctatggttgc tggtgaagag
     3481 gtagttccag aaaaagaagc gaaggatgaa aagacagttg ctaatgggta ctttaaagat
     3541 gccgatgtga aggatcgcga attgagtgat tatacaggcg aatggcaatc tgtttatccg
     3601 ttgttaaaag atggaatttt ggatgaagtt ttcgattaca aagcaaaaat caataaagac
     3661 atgacagctg ccgagtacaa agattattat actactggct ataagacaga tattgatact
     3721 atcaacatta aagacaacac cattgatttt gtagtcaacg gtgaacatca tcaatacaca
     3781 tacaaatatg ttggatacaa aattttgaat tatgaaaaag gaaaccgtgg tgttcgtttt
     3841 aattttgaaa ctgatgatgc aggtgctggt cgttttaaat atattcaatt tagtgatcat
     3901 ggaattgcac caagtaaggc agagcatttc catattttct ttggtggaga aagtcaagaa
     3961 aaactctata atgaattgca taattggcct actttctatc cagcttcatt gtctaaacat
     4021 gaaattgctc aagaaatgat ggctcattaa aattcaattt ttggaggaaa tacatggtaa
     4081 gaaaagtatt attgtctatg gattccaaga atattgatgc ttatcggcgt tatcgatttg
     4141 caaagcaaat gagattaaat gggagtacat tagctgatat tgctgcaact ttacatgtta
     4201 gtattgacac agttcataga gtattaaaac gggatcctga ttgttgtttt atcaaggtca
     4261 atactggaag aagtagacgt ttagcaaaac ggcataatag cttggccaat taattgataa
     4321 taccagagta gatcattttt cccttatttg gatttgatct gctttttatt atgtcttgaa
     4381 ttgtcaaatc aagcgcaaca ctttatcaaa gaagacttca taaggtgttc tccaatctaa
     4441 acactttcta ggtctgttat tcagtttttc aatcatctga tttatcgttg agttatctac
     4501 cgtagctatc tctttcgatt ttggcaagta ttctcgaatg agtctattcg tattttaatt
     4561 tgttcattgt tgccaagggc atgaggatct ggaaaataaa agggcatatt attcaatgct
     4621 tctgtaactg cgaaatgatt ggagaattct tttcctctat ctggagtgat cgttttgacc
     4681 gcctcatttg gaatactgct taaaagttca atcattttct cgctcacata agcggctttc
     4741 tttctgggga ccttctttcc tgctaaaagg aagcatgatt ttctgtctgt caaagtcact
     4801 aaacaagtac caccagtttt gccagctaca atatcggctt tccaatgacc aaaatcactt
     4861 ctatcatctg cggattttgg acggccatgg atcgaatgag taaggcaaat ctttccacga
     4921 gactcgacat gattttttgt atgtcttgtt tttcctcgat ggtgcaatga acggatcagg
     4981 cctttattgc cgtgtgagag gggttctgta tcgaaaagat cattgcaaat tgcacgttag
     5041 atcgtgataa aactaattga aaagtccata tgttcatgct tcaaacgatt cgagatttcc
     5101 tctggcgacc actgaaaatc gacaaataaa tgacgaacaa ttgaccaaac tcacggatca
     5161 tttagaatgt gataacgtcc gcattttttt cttcgataag cataattttt ttccgctttg
     5221 gaaggtgaat agtttttttc ttgtttcttc tcagttcatg taaaatagta aacgggctat
     5281 gctgaatgtc gttgccaatt atactaatag aaaatccttc ttcgtgaagc agaaagattc
     5341 tttctcgttc gagtatagta agatgacgat actggctcat aattattttc cttctaggag
     5401 tgttctcgac aaacttatac tagttggata tcgattatga ctcattttta tgtgttgcac
     5461 ttaaagtgta aattcaaggt atagaaagag gttccctatg aaaaaaatac ctattacaat
     5521 cttaactggc tatcttggtt caggaaaaac gatacttcta aatcatattc tgtacggtga
     5581 tcacggaaag gaaattgctg tcattgtaaa tgacataggg gaagtaaatg ttgatggggt
     5641 attagtcgac aagaaaggtt ttaaaagaac tggagaaaaa cttgtttcga tgccgaatgg
     5701 atgcatttgt tgtacattac gcgaagattt gattgttgaa ctagatcaat tagtgcaatt
     5761 ttctgacttg aatcaaattg ttattgaagc ttcaggaatc agcaaaccaa ttccaattat
     5821 tcaagcaatt atattagcta aaaacccttt agacattgat ttgacccaac gattagagat
     5881 tggtgccatt attatagtag tggatgtaaa acgtttttgg gataatttcg aaacgggtga
     5941 aaagattcag gaaagaaaag ctgaaggtaa tgtagttgaa gaagaacagg atattcgtgg
     6001 cttgttaatt gatcaattcg agtgttgtaa catattactt ttaaataata gtgacttagt
     6061 gtcaaccaag acgatttaca aaattaacgg tctaattgct aagttacagt ccaatgccaa
     6121 aataattagg acaattaaag gtgaagtaga cttatcgaaa attctaaata caaatctttt
     6181 taatatagat gagacttcct attcagtggg ctggttcaaa gagttagagc ttggctatca
     6241 aaatcatatt cctgaaacag aagaatatgg tatctcaagt tttatctatc gaaatcggtt
     6301 actccttgac gcacaaaaat ttgcccatct tacggataaa aattttcctg gcaatattac
     6361 aagagcaaaa gggtttcttt ggtttgccca acatagtgat ttttcgacga tctttgaaac
     6421 atcgggtagc tataaaagta ttgaatcatt aaattattgg gtagctagtt tacctaaaat
     6481 tgagtgtcaa atgattatac aaaaaaatcc cgaattgaag gaagcctggc atccgttgtt
     6541 tggtgaacgc attaataaac tagtttttat tggtatgaag atggatcaac aatgtattaa
     6601 ggaacaactt gagggttgtt taactgattc tcttaatgca gataatttcc gaaaaatttc
     6661 accatttcca tggaaaaagt ctaatatgtt tagccagata tcgttaatga aatttttaag
     6721 aaaagctggt gtcaagatag gcgatgtcat aattattggc attttgatta ttacctcttt
     6781 tattccctta tttgtattct ctatagaaaa ggagaatgaa acaactgaag atgctatcaa
     6841 atatgccatc gttcgaattg atggcaagga aatggatcgc ttcgatttgg ataagatcaa
     6901 tcataaactc gtgaattatc atcctgcgaa gggacaatat aatattgttg agattaaaga
     6961 tggaagaatt cgagttaaag aagataatag tcctgatcaa atcgcggtta gaactggatg
     7021 gatctcaaaa cccggtcaaa cgagtatttg tttaccccat aagttagtta ttagtattga
     7081 gaaaaaagaa agcacagatt actacattta ctaatctgga aatagatgag gaggttttta
     7141 ataagaaaaa gatattatta ttaataatag caggggcttt attaagtgtg ttaagcgctt
     7201 gtggacaaga aaaagaagcg gtgacaacag gcacaagtat ctttaacggg acctgcaatg
     7261 gtttagttaa cagtcggtta gattacttat tttggctctt cgtcaatgag tgttggtgaa
     7321 gaaattcaat agaataatcg agatactcac acggctagac agctatcttg tctagccgtg
     7381 ttgttgttct ggctttagtc agttgggtag tattcttagt tttcttaaaa tattttcctc
     7441 gatataaaaa gattcgttct cggtagtttt gaaagtttct atagccatag gcaattcgtt
     7501 ttagtacttt gatatggtta ttcaaacatt ctatagggcc gttggaatag ggaacgcgca
     7561 acgctcgttt gatttctgtt tggtatttct tgaaggtcgt gaacactggt tggtagtgct
     7621 ctggcaactc cttgtagtct tcttttagga gcgcgacgaa ttcaggaaca tcttgtcttc
     7681 tgataacgga aagaaaatcc tggtagactt cgtagcctcg ctttaaggaa tcatcataag
     7741 ataatagccg atcgactatc tctgcttctg tcagatgatc tcgaaatgac ggacgccagt
     7801 agcgatgctg acctttcaat tcccacgcat tcttttgaag tagtttccag tatttcttta
     7861 agtgcttcga ccgacgatcc ccttttttaa aggtcttcat tacttgaatt cggtggttgg
     7921 taaaagcccg accaatgtgt tggctaatat gaaagcgatc ggcgacgatt cgggcattcg
     7981 gaaaacactc tttcaccaat gtgcgatagg gtgtatagaa atctgaaacc actagacgca
     8041 cttgttcacg gacttttata gggtagcgca agaaatagtc gcgtaaagga ttgagttgcc
     8101 gattttccac aatatcaatc agttgatggg tcttgccgtc catcatgaca aagctcatgg
     8161 ctcccgagac atttttcact gatttgaatt catcgaaaca caccacttct ggtaacagct
     8221 ggctcaaaga aatggtttta ggccgaaggc tgcgaagtat tcgaatgaca gtggtcggag
     8281 aaatgtgctt catacgtgcg atcaaagaca tagaaagagg ttcgcttaat agctcaagaa
     8341 tggcttgttt gactctgcga gcaatggagc agcgacgatc actaactgaa tcttctgcta
     8401 aaaaagtctt tccacagtgt ttacatttga agcgctgttt gttaatccgc agaatggtct
     8461 tgtagttact gacatcattc aataagatgg atactttttt ccagccctat ttaatgatcg
     8521 tttgttcatt ttgatagtgg cgatgaaagc attcctccgg ttgataggtc aatgtgccat
     8581 caaaaacaaa acatgtttcg tcattgattc gctctttgcg aaaggcatct tcatgaaaga
     8641 taagatttag gtctagaata tctagtaact ctttggtaaa ctctgaatag gacatcttga
     8701 aacactcctt tgaatggttt tcgtcgactt tattctaaag gattttaagg tgtctgtttg
     8761 cgtttattag aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca ctagatatta
     8821 tagatccctt attttcttcg cttcaaattg tcaaggagtc atcataaatt ttgtatggat
     8881 tcttttgttt gggtagtagt cttctaaata atcttggatt aacaccttca tctctatttt
     8941 acttttatat ttgttggagt cgattaattc tcatttgatt gatcgatgaa acgcttcaat
     9001 tactgcattg tcaacaggag tcccagtttg gctcactgaa tgtgtgatgt ggtggtcgtt
     9061 caacagctct tgataaataa aagaccgatg ctggctacct ctatcggaat gaattaccgt
     9121 aggcaattct ccttaataaa tttgtaatgg acggacgaca atctcttttt tcatgtgtga
     9181 atctaccata tagtaacgta gaatacgagt tgctaagtca atataggtgg acaaatagag
     9241 gcgaccgtca ttacatggaa tgtaagtaat atccatcacc caaacagcat caatttggtt
     9301 ctgttcaaat tatcgattca acattttttt accatttctt tgattgtttt ttctctacca
     9361 tgagaagatt tgcgtcgatg aaatcctctt gtgcataaat tcatttcacg cataagaaca
     9421 gctacaacac gtttattgat ttcgattcct tcatcgtaaa gtttttgagt gatccctgga
     9481 gatccgtatc gatctttgtg ttcgtagaac actcgtcttc ctcttttagt cgtttatttt
     9541 cctgttcaag aatgtagaaa tctgctttat caggtaatct ttttccttgt ccagcaaaag
     9601 catttttccc gtatcgacga tattctgaaa cccatcggga aattgttgga cgagagatat
     9661 tgtattcttc agcaattttc ttggtagtta cttttttctc aaggatatcc tgtgaaatct
     9721 ttagtttaaa tttacgatca tatgtttttc tcaatagagt tcattccttc cggtaacgta
     9781 actgatagtc aagaaaagtg tatcatagcc caacttagta tcagggtttg tcaactaaac
     9841 tgtggaagtt gatagataag agtttttaac aaccaaaatt ctcttgacta tttttgaaat
     9901 cagacaaatt ttgattggac acatagttga ataaatctac cgtcgttacg gaaggtaaat
     9961 cgcatgcttt tcaattctag aggagaagtc ctcgttgaag taccatagcc cagttctgga
    10021 ttcgacctcc tgcccacttc atacgtaaat aaagtaattt taggagtgag actgtgacaa
    10081 aagttatttt taagctaaaa acgaattaaa aaacgttgat aattaaaatc attcgtttta
    10141 gattctatga atcattatag aacgctgtaa aatcgatact ttataattat aaagaagata
    10201 gtttcaaatt tattgttcga aatttgaaac tatcttttta cttatgtcac agactccctt
    10261 agttgagtta ttctttaatt tcgaatttcg agatagctag atatattgca agcatacatt
    10321 cttgataaca atcttgatca tattttccag ttctccagga ttgcttatga atcaaagagc
    10381 taaatctttt gagaattgcc tcaacagctt cttcatttcc agcctttgct aaaatgaaat
    10441 cttcttttaa agaattcata aatctacctt cttcccatgg acttataatc tatttctttg
    10501 ggaaggagga gaacgagcaa agaagcatac aaaattagtt attatgcaga tattaaacca
    10561 gcattttttg aatttttttg taaagtcgat gttttaaatt tgtcgcaccc tgtcttgtaa
    10621 tccccagaac ctttccaatt tctttatcag ttttatcaaa gataaatttt tcgataagaa
    10681 aaatttgttc aggttctttc aacaaaggaa ggatttttgc taattttata tcttcaacaa
    10741 ggagagtcac atttaaaaca ttaatcggtt gtttaaattt tatagtatct ttagtaaaca
    10801 aatatggtgg gagttgatca agttgttcat gtttttgaat ttcttcttgt tttttataat
    10861 aagtattagc ggtgttcaaa atgacctttt caaagaatcg aatcaactgg gcatttacgc
    10921 gttcgtcgtt atctgacata ttgcacacct cctccattaa gatttaaagg gaaaagaggc
    10981 aaaaaacacg aaaagaagct aatgcaatta tgctgcgctg cgaataactt ctataagctc
    11041 acatgcattg ataggactgc tgatgtttat tttcaaaaat gtttttttag ttgataatgg
    11101 atagttatct ttatacttag aaaagaaaat tcatatatct tcttaaatat caaacatgtc
    11161 cttatggcta agaaaatgtc ttgagctttt tacgtataat aaatcttttc taaaacctcc
    11221 ttttggataa tattcttatt cgtgtataga ttgtattttt tatttcttcc cccttcataa
    11281 ttaacaacta gaaaagagtt cgaatagaca cgtatttgaa cgaaaatttt tattggattc
    11341 ccaaattgga atatttagaa acgaaaaatt cctattatct gtatagtgct tagataataa
    11401 cggatcataa taattattgt ctttgaacta gatatatgta aatagtattc atcatgatga
    11461 aaaatgatga gttctcggtg ataaaatgta ctttttacgg tggaatatac atgaaagcga
    11521 taagtggata ttttataatg aagtaagtta gcttattttg acagaggaat agtattttag
    11581 ataacgtata aaatactatt taatgatgta gaaaggaaag agagaaacaa aagtgttttc
    11641 agataaagaa gtcttattaa agtctcttca tgtttgttta aagttaccta tcgtggtttt
    11701 taattccgaa ttcagtatta taaaagagta tcgctcagat agaactactt atttttttta
    11761 tgattataaa aagttactaa ctgataatat taaaaaaact aaagatttcc atttctttac
    11821 tgg
//
LOCUS       DAZKOB010000087        11556 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000087 DAZKOB010000000
VERSION     DAZKOB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.087"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(364..1482)
                     /locus_tag="ACG8M6_002230"
     CDS             complement(364..1482)
                     /locus_tag="ACG8M6_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457854.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1715..1837
                     /locus_tag="ACG8M6_002231"
     CDS             1715..1837
                     /locus_tag="ACG8M6_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457855.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1953..3260
                     /locus_tag="ACG8M6_002232"
     CDS             1953..3260
                     /locus_tag="ACG8M6_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0457856.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="ACG8M6_002233"
     CDS             3379..4578
                     /locus_tag="ACG8M6_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0457857.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="ACG8M6_002234"
     CDS             complement(4605..5873)
                     /locus_tag="ACG8M6_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0457858.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5928..5994)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6036..6682)
                     /locus_tag="ACG8M6_002235"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="ACG8M6_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="ACG8M6_002236"
     CDS             complement(7090..7593)
                     /locus_tag="ACG8M6_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0457859.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="ACG8M6_002237"
     CDS             complement(7716..8480)
                     /locus_tag="ACG8M6_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0457860.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="ACG8M6_002238"
     CDS             8588..8863
                     /locus_tag="ACG8M6_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0457861.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="ACG8M6_002239"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="ACG8M6_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0457862.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10609)
                     /locus_tag="ACG8M6_002240"
                     /pseudo
     CDS             complement(10430..10609)
                     /locus_tag="ACG8M6_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11435..>11556
                     /locus_tag="ACG8M6_002241"
     CDS             11435..>11556
                     /locus_tag="ACG8M6_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457863.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
BASE COUNT         3493 a         2423 c         2037 g         3603 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaataacg
    11341 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
    11401 tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga
    11461 cgaactgaca atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa
    11521 ccgaatggga cgtgctatac aaactattta taatgt
//
LOCUS       DAZKOB010000088        11457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000088 DAZKOB010000000
VERSION     DAZKOB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.088"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..1225)
                     /locus_tag="ACG8M6_002242"
     CDS             complement(119..1225)
                     /locus_tag="ACG8M6_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0457864.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     regulatory      complement(1399..1565)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(1790..1996)
                     /locus_tag="ACG8M6_002243"
     CDS             complement(1790..1996)
                     /locus_tag="ACG8M6_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457865.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2012..2626)
                     /locus_tag="ACG8M6_002244"
     CDS             complement(2012..2626)
                     /locus_tag="ACG8M6_002244"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0457866.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2773..3456)
                     /locus_tag="ACG8M6_002245"
     CDS             complement(2773..3456)
                     /locus_tag="ACG8M6_002245"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0457867.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3565..4023)
                     /locus_tag="ACG8M6_002246"
     CDS             complement(3565..4023)
                     /locus_tag="ACG8M6_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0457868.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4107..5870)
                     /locus_tag="ACG8M6_002247"
     CDS             complement(4107..5870)
                     /locus_tag="ACG8M6_002247"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0457869.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6091..7551)
                     /locus_tag="ACG8M6_002248"
     CDS             complement(6091..7551)
                     /locus_tag="ACG8M6_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457870.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7759..8685)
                     /gene="glsA"
                     /locus_tag="ACG8M6_002249"
     CDS             complement(7759..8685)
                     /gene="glsA"
                     /locus_tag="ACG8M6_002249"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0457871.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8708..10135)
                     /locus_tag="ACG8M6_002250"
     CDS             complement(8708..10135)
                     /locus_tag="ACG8M6_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0457872.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10166..11380)
                     /locus_tag="ACG8M6_002251"
     CDS             complement(10166..11380)
                     /locus_tag="ACG8M6_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0457873.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3462 a         2384 c         1896 g         3715 t
ORIGIN      
        1 taatatgatt ttttgagata ctttcattgc gaattcctct ttctaaaaga acctctcgct
       61 tgaaaaataa aagattgtac tttatgataa ttcaaactga gagtttctca gtttctcttt
      121 attgtttttc aggtcgatag agcgccaact cttcggcctg tatctttgaa gaaatattca
      181 acgtgattcc gattcctaaa gaaaggatca gataacttgt tcctccgtag ctaacaaatg
      241 gcaatggcac accagtcatt ggcatcagcc ccgcgatact tcccacgttc attatcgtct
      301 gaacgaatag caaagttccg aatcctaaaa gaaacaatcc agcttgctga tttttacagc
      361 gactgcttaa acaaaagatt ctcatgcata atgagaacaa taagaaaagc acacacaatg
      421 caccgattag cccaagctct tctgtaataa tcgaaaaaat aaaatctgtt tctgtctctg
      481 gaagataccc tttttttgtg atactatttc ctaatccccg cccaaatatg ccaccattat
      541 atagtgcata aaacgaatgg gtcatttgat agcccgcacc atgactatcg ataaaagaat
      601 ctcttaaagt cgcgatcctt tcataagcat gattaaacat ctgtggaagc caacctttat
      661 ttcctaaata taataccccg cctgctgctc caatcagcaa tgcagaaaaa gtaacgatta
      721 gatagatccc ttttttaaat ggaaccgccg ctgcccaaaa tatgacccac gcaatcgaaa
      781 ggatcatcaa tgctccagca atctttggct gaaataaaat cagaaaagtt atacctactg
      841 taatcaggaa tggttttttt agatcttttg ggctactatt tccgtcacga aaaaaccagc
      901 ttaaataaaa aatcaaaaaa agatttgcca gttcagaagg ctggaattga atgccaaaca
      961 gagagatcca acgttgtgcg ccgttgacag tgacaccgaa tatccctatt cttactaata
     1021 ctaaaaagaa aatcgataag gctaaaccgt atcctgctat tttagggtga agtaaatagt
     1081 gtagtttgac tgaacgagct aagaagatca cgctccaact caaaaatatg aaaattagtt
     1141 gacgcaataa tagacttttt gtattttcat cggcttgcaa taaacgataa gaactggcac
     1201 tgtatacttc taaaagaccg atcatggaca aagtcaaata aggacccaaa atccaccaat
     1261 cgatcttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg tgaggagcga
     1321 gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga aaccttttcg
     1381 aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat acaacgtaaa
     1441 gaaaacattc ttagctattg gaaaaagcct taatccaaca gtttctgttt tacccattgg
     1501 cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac ctaacaaggt
     1561 tattactata ttctatttac gagttcatga tagacttttc ctaatgatta ggcaatggaa
     1621 tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa aataacaaat
     1681 aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt gataattttt
     1741 tagttttatg tgttttttta tatgtttttt cacttttcgg aataaatatt tatttgttaa
     1801 agtaattaaa caatccgcta atcgtttgat ctggcatttc tatttcctta ccatctaaag
     1861 taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac tgcccttgca
     1921 gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc gatggtttat
     1981 agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc gtgaaaattt
     2041 ctcctattgc tgcataatca tttcctgcca aacggcttac agcattcaat tttcttggat
     2101 cgatccttcc tgtcttatca tcatagactg tttcatttaa atgatatttc tgtacttcta
     2161 gcaaaaataa atcagctgtt actttatttt tattccgtat ctcgacatgt tcatataagg
     2221 atgtttcaaa acgaactttt gcttctgtca atgctggtac tgaaatgatt actgaatcag
     2281 ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt aggcagattg gctgctgttt
     2341 tatttacttc ttctacgttg tcctcatcaa ggatatggat aacagcttct cttgtctcaa
     2401 gaatattctt tgctgtgtct tttactgcat cttgttttct ctggatcgcc aaggataaga
     2461 tgggtcgatt cgaactgacg atattgaagt aactgaaggg tgcgatattc actatatctt
     2521 ttgctgattt agtcgttact actgctactg gtctagggat gactgaacca atcagcaact
     2581 tgtaattttc acgttctgtc aattctgaag gactccgttc aaacatggtt tcgccttctt
     2641 tctataaaat tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat gcttttttca
     2701 tcttcttaga atctgcttta aactatctat caatttttct tttttgtcaa gaatctgtgc
     2761 cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg acatatcttt tagtgatcaa
     2821 ttgcggacgg gtgataatag atccaactac gactgaaaaa gcacctaatt catatgcttt
     2881 ctctacttgt tcaggagagt cagtatgtcc ttccataatt actgggatat cgacttgttc
     2941 tatgatttga ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat attctgtata
     3001 gcctgctaaa gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa gcgcttcctc
     3061 aaaagtcgaa atatctgcca tagctaaccg accgtgttcg tgaatcattt ggacaagttc
     3121 agcagttgtt ttttctgtcg gacgtttacg agaagttgca tccagtgcaa tgatttctgc
     3181 ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca gtaataaaca cttctgaccc
     3241 atcgtaatct cttttgataa tccctattac cggtaaatca acgattcgct tgatttcatt
     3301 gatatcttct gtcgtatttg ctcgaatacc aactgctccg ccttcttttg cagccaaagc
     3361 cattctcccc ataataaaag aactgtgtag cggctcgttt tccaaagctt gacaagaaac
     3421 gacgactccg cctttgattt gttccagtgt atccacaata tctcctccag taatgttata
     3481 taaaaataca gcttcatata aaaatgtaac ccttttctat tcaggagaca ataatgagct
     3541 atttctttca gcagaccttt cttactaaaa gaaaattact tcaaattctt cgtatacgac
     3601 cagcttctct tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt taaaaaactc
     3661 agcatggact ttccgccaat aagctaatgt ccgatcccct tctccttctt tccaagcatg
     3721 ctctttcgtg acttcactaa aaggcaagat ggtcacattt atatttttga tgatacaaac
     3781 tggttcattt tttccattca ggaccacgtc taattgacca acttttggta aaggctcatt
     3841 ctcttgttta tacagatcgt aaccacttga tgtaccagtc tttatctttt ttacaactag
     3901 ttcgctcaac tcgtctgcca ttttttctgt atccccaaat gaaaagaaac ctaatttttc
     3961 tgattgataa ctaggatatt tttcttggaa ctttttgaca tattcttcta cttgctgata
     4021 cataacatcc acccctttgc tttttataat gaaaaaacca tccagatact agtataaaca
     4081 attatctgga tggtgagcat aatgaattat tgaatattca agacacgcag actatcttca
     4141 acgatttgtt ttggtgttaa atgataacgt tcataaagct gccctacagg aacgctatcc
     4201 gtgaattctt tctctgcacc aaaatttaat acgttcattg aagaatcacc ataataacga
     4261 ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat gactaattcg
     4321 tgtgtttctt ttaatttatc taatgtttct acgtcatttt ctgtgatcgt tcctggattg
     4381 atcaacgttg cttcgattcc tttttttcta aaagctttta ctgtttcttt tcctaattca
     4441 agaaaaccgc ctaaagctaa aattgctact tttcctccat tatttactgt ttgataggct
     4501 ggaatactat aatcatttgc gatagctgta ccatgttcta caccatgtgt aggaattcgg
     4561 attgctacag gctgcgctgt ttgttcgaac gcccaatcta gcatagagat catatcctct
     4621 tttgttgctg gagcaagata tttgatgttc gggatacttg ataagtaagg aatgtcaaat
     4681 gttccttggt gtgttcggtc actagctgag atcgttccgc caccgactaa aataatcact
     4741 ggattctcgt tgatcgctaa atcatgtgat aattgatcat atgcccgttg caagaaagta
     4801 cttgattgga agacgactgg acgtactccc tgagaagcca aagcagaagc gaaagtgata
     4861 gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg gatgtttttc ttggaatgtt
     4921 ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg caataggagc tgtacttgtt
     4981 tgcattttct tatccaaata atccatgatg atcatattaa atgtttctcc agaagttact
     5041 tgctttgcct ccccggtgga cagatcaaat ggtacatgcc aatgatattt cattttatta
     5101 tcgattgccg gctgataacc gcgacctttt tccgtatgga cgtgaagtac aatcggatga
     5161 tcaatatctt ttacttcttc aaataaacgg atcaccgttt ccagatcgtt tccattatcc
     5221 acgtaacgat aatcaaatcc cattgcttta aataggttat tgctgctttt accgttggtt
     5281 tgacgaagct cagctaagtt tttatataaa ctgccatgat tctcatcaat agacatctca
     5341 ttatcgttta ctaatacgat tagattggat tgtaaaacac caccattgtt caatccttca
     5401 aaagctaagc caccacttag ggagccgtca ccgataaatg ctaccacatt tcctgattct
     5461 ttcttcagat ctctcgcttt agctaaacct gttgctaagc tgatagatgt agatgtgtgc
     5521 cccactgtaa aatagtcatg tttactttca tgttggttgg aatatccgct cacttcacgg
     5581 taatgtcctt tttcaaaacc attttttcga ccagttaaaa gcttatgacc ataagactga
     5641 tgggaaacat cccaaacgat tttatccttt ggtgaatcaa atacataatg aaacgcaatc
     5701 gttaattcta ctacacctag attcggacca acgtgcccac ctacatcgct gtccttttgc
     5761 aagatttttt ggcgcatctc ttctgccaac tgtttcattt cctcaacaga taattttttg
     5821 acatctgcgg ggctattgat tgcatccaat acatgtaata ctgtactcat aatacattct
     5881 ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt acactttatc tattctacta
     5941 cttagagttg actctaagtc aatagcattt taaaaaaatt tcagagaaag cttgttatat
     6001 aagtagatct tcgcacgaat caggactcaa ataaaaacct tctacagaca attaaaaagc
     6061 tttgtagaag gtctcctaat taaggtctca tcaagaaata tgttttttta ataggtgaag
     6121 ttcctgagca atatgataca aatggatgtt tgcacctttt aatgagattg gatagatctt
     6181 tgtcttttca gtttctaatc cattttgata gctagcagca tcgcacaaaa ccactctttt
     6241 tttcgcacat agacattcat caggcaattc ttctaatgaa cgaaataaaa taggatttat
     6301 ttcagcgatg ccttcttccg catacaaatg aaattgggtt cgataaaaaa attgtttgac
     6361 tgaactttct gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt cactgttgat
     6421 caaagaaaga cttttgacga attttcgaat gaaattttca ttccatctta gttggatttg
     6481 atttttcttt ttccactctt ccaatatttc tacaattttt ccgtacagaa gttgttcttt
     6541 ttctgataga agatagatcc tttccggtaa aaatacttgt ccatctgccc acaacgtacg
     6601 gacaaattgt aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga tttcagcgat
     6661 actaataatc tgtaaaatca attcattcag tgataggtca ttgagaacaa aatttttata
     6721 aacaacttcg aaatcttttc ttaacagctc catgttctca ttggtataat ttctactgtt
     6781 gaacaaagaa aaaatataat agacatcttc ttcttgtaag tctagaattc ctttttgctc
     6841 ttccaatcct tgacagacta attgaaataa cggcttcgtt tctgcgcttt tcttatcttc
     6901 ttctttcagt ccaaggggaa atgcctttct tccgattgcg atctctattc ctcgtatgat
     6961 aaaggacttt tcatcttccg aatatcttct tttaaaaaaa tgagtctcta cataatcaat
     7021 caactcgtta caagcttctt ctacatacgg atttttattt tcatagcctt cccaattgac
     7081 ataacgtact aaagctagca agatgttccg cgagtcaaat tctgggataa tgatttcttt
     7141 atttttcaaa taatcatttt ctttaaaaaa atccaatacg atttttttga ttgtatatac
     7201 tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata taccattaaa
     7261 ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg tgagctcaga
     7321 aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta cttgttcttt
     7381 tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg ttggtgtagt
     7441 cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa ataattgttc
     7501 acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca ttcctgatac
     7561 acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta tttttgtttc
     7621 ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg acaaaagtca
     7681 aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga cttttacccc
     7741 agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga ctacatcttt
     7801 caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc tgaagacacc
     7861 tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc tttttgcagg
     7921 tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag ttgtcataat
     7981 tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc aaggagcaat
     8041 tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga cattgaggga
     8101 gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca tttgattacc
     8161 tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag aatgatagat
     8221 ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg acaagattcg
     8281 ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc
     8341 attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca tgatagaatt
     8401 gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta ttccgttgtc
     8461 tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa agcgcacatc
     8521 ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca tacctaattg
     8581 tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg tttgataata
     8641 gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa taaccagctt
     8701 tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc gtaaatggaa
     8761 taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca tgctgtccag
     8821 ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac
     8881 ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag gctctttcaa
     8941 gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag tacaacatca
     9001 ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc cccccgccgc
     9061 ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc atcaaatgag
     9121 caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct tcttctgctg
     9181 ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg atgacaccta
     9241 aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca atgtgtctta
     9301 ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt tgaggtactg
     9361 tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata accaatacaa
     9421 acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga ctggcagatg
     9481 tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg
     9541 aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg cctgaagcga
     9601 cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca gctacaaaac
     9661 cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc
     9721 agaaaataat caataggatt gccaatttca gatatttatt gttttgtata gcaccaatac
     9781 ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag ccaactgtta
     9841 tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat gtgcgactga
     9901 cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct gctgcgcaca
     9961 atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact aagctgaagc
    10021 ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg gtcatagcca
    10081 aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt tgcatgaatc
    10141 ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca
    10201 atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc gccgatttta
    10261 aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca atcacttgaa
    10321 tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat gcaaagatcc
    10381 ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca tcaagtgcat
    10441 catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata cccgcaatga
    10501 tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta atggctgcca
    10561 atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct tttacttttg
    10621 caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat
    10681 tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac cagccaaacc
    10741 aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt ctttctccta
    10801 aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat ccagctgtcg
    10861 tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa aatccgccac
    10921 cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata acaagtataa
    10981 tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga acggtaatca
    11041 cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca
    11101 tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt ccaataacac
    11161 cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata atcgttgtta
    11221 tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc cgtgctaatc
    11281 cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta cataaaatca
    11341 tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc tcctttctac
    11401 gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg aattaat
//
LOCUS       DAZKOB010000089        11198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000089 DAZKOB010000000
VERSION     DAZKOB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.089"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8M6_002252"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG8M6_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0457874.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8M6_002253"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG8M6_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0457875.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACG8M6_002254"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACG8M6_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0457876.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8M6_002255"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG8M6_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0457877.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8M6_002256"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG8M6_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0457878.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACG8M6_002257"
     CDS             complement(6343..7062)
                     /locus_tag="ACG8M6_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0457879.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACG8M6_002258"
     CDS             complement(7059..8372)
                     /locus_tag="ACG8M6_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0457880.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACG8M6_002259"
     CDS             complement(8453..8773)
                     /locus_tag="ACG8M6_002259"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0457881.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACG8M6_002260"
     CDS             complement(8881..9168)
                     /locus_tag="ACG8M6_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457882.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACG8M6_002261"
     CDS             9295..9939
                     /locus_tag="ACG8M6_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0457883.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACG8M6_002262"
     CDS             complement(9998..10705)
                     /locus_tag="ACG8M6_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0457884.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACG8M6_002263"
     CDS             complement(10925..11095)
                     /locus_tag="ACG8M6_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457885.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1957 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaacg
//
LOCUS       DAZKOB010000090        11122 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000090 DAZKOB010000000
VERSION     DAZKOB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.090"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..1343
                     /gene="lysA"
                     /locus_tag="ACG8M6_002264"
     CDS             84..1343
                     /gene="lysA"
                     /locus_tag="ACG8M6_002264"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0457886.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1330..2130
                     /gene="dapF"
                     /locus_tag="ACG8M6_002265"
     CDS             1330..2130
                     /gene="dapF"
                     /locus_tag="ACG8M6_002265"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0457887.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2133..3014
                     /gene="dapA"
                     /locus_tag="ACG8M6_002266"
     CDS             2133..3014
                     /gene="dapA"
                     /locus_tag="ACG8M6_002266"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0457888.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3011..3727
                     /gene="dapB"
                     /locus_tag="ACG8M6_002267"
     CDS             3011..3727
                     /gene="dapB"
                     /locus_tag="ACG8M6_002267"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0457889.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3733..4428
                     /gene="dapD"
                     /locus_tag="ACG8M6_002268"
     CDS             3733..4428
                     /gene="dapD"
                     /locus_tag="ACG8M6_002268"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0457890.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4444..5445
                     /locus_tag="ACG8M6_002269"
     CDS             4444..5445
                     /locus_tag="ACG8M6_002269"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0457891.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5442..6803
                     /locus_tag="ACG8M6_002270"
     CDS             5442..6803
                     /locus_tag="ACG8M6_002270"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0457892.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6763..7914
                     /locus_tag="ACG8M6_002271"
     CDS             6763..7914
                     /locus_tag="ACG8M6_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0457893.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7889..9058
                     /locus_tag="ACG8M6_002272"
     CDS             7889..9058
                     /locus_tag="ACG8M6_002272"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0457894.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9233..10612
                     /locus_tag="ACG8M6_002273"
     CDS             9233..10612
                     /locus_tag="ACG8M6_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0457895.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10893..11122
                     /locus_tag="ACG8M6_002274"
                     /pseudo
     CDS             10893..11122
                     /locus_tag="ACG8M6_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3702 a         1945 c         2493 g         2982 t
ORIGIN      
        1 taatattatt ccaataaaat tcaaatcttt atcaaatgaa attccaataa aaacggaaaa
       61 aaagtgaaaa gaggaatgaa aaaatgacag aattgataat tggcggagtc acagcaagca
      121 cattggcaga aaagtataag acacctctgt atgtgtatga tgaagaaaag atgagacaaa
      181 cgatgctgtc attcaagcga ggctttgtat cgaaacgttt tgatacaaaa gtgctttacg
      241 cctctaaagc attccaaacg attgagatgc tgcatttagt tcgtgaatat ggattcgggc
      301 tagatgttgt cagtggtggg gaaatctata ccgctttgca agctgatttt cctagagata
      361 ctatctactt ccatggtaac aacaagacgc cagaggaact gatctatgca cttgaaaatg
      421 atttgttgca tattgttgcc gataatttga tggaagttga attattagca gaattaagcc
      481 aacgctattt aaaaaaaatc tgtatcatgc ttcggttgaa tgtcggagta gaagcacata
      541 ctcatgaata tatcgtcact gcgcatattg attcaaaatt cggcatgagc tatgaaagta
      601 aagagtgcca acaagccctt cagttgataa aagaaagcag ctatctagag ttagaaggat
      661 ttcatgctca tatcgggtca caaatttttg atgttactgc ttggttagct gaaatcgaca
      721 aattgacgaa ttacttaacc gattttgacc aacctctttc cttgaatctt ggcggagggt
      781 ttgggattcg ttatacacaa aatgatcagc cgatcccagt cgaagaggca ctaaaaaaac
      841 tgattgaata tacggaagaa gctttggaaa aacgaaaact gaccattcgt caattactga
      901 tcgaaccggg tagaagtatt gttggagaag ctggtacaac actatacacg attggtttca
      961 tcaaaaagac accacataaa aaatattact ttgtagatgg cgggatgaca gataatatcc
     1021 gtccagctct gtatcaggca gaatacgatt gtgatttagc agcaaaactg tcggaagaaa
     1081 aaacagagaa agtgacgatt gcaggaaaaa tgtgtgaatc aggcgatgtg gtaattaaag
     1141 aagcttattt gccgaaagca gaatcgggtg atcttttagc tgtttattcc acgggagctt
     1201 atggatactc gatgagcagc aactacaatc gagcgacacg accagcagtt gtttttgtca
     1261 ataatggcca atcaagactg attgttcgca gacaagcatt ttctgatcta ctaagggggg
     1321 aagtttccta tgatccttca taaatataat ggctgcggaa atgattttat cttattagat
     1381 tacgcagaag acacagatta tagtcaatta gccgcttctt tatgcacaaa agaaaaatat
     1441 gatatagacg gattgatcgc cgtaaaaatc gatccgttgg aaatgatcta ttacaacaaa
     1501 gatggcagcc gcgcgccgat gtgcggaaat ggcattcgct gttttgctcg atacgtaaat
     1561 gatcagaaca tgatgaatga acaggaattc aacgtagaga cattagcagg cacaagaaaa
     1621 attacggtgt tggatccctc acctttttat tgcagcgtag atatgggagt gccagatgat
     1681 tctccggaaa aaatcaaagc taatcaaact tatccaataa aaaaccagga aatagagatc
     1741 aatggtgaaa aagtaacgat cactagttta tttttaggaa cgattcatac cgttgttttt
     1801 gttgaaaatg cgcgtgagga agtctggaga aaaagaggtg aactgatctg tcatcatcct
     1861 ttgttcaaag aaaaaaccaa tgtcaacttt gtagaagtag tcaatgaaca agaattgatc
     1921 gtccgtacct atgaaagagg agtaggttgg acacttgctt gcggtacagg ttgctgtgcc
     1981 tcttatgtgg ttgctaagga aactgggaaa atttcagcag atcaggtagt tgttcacttg
     2041 gaacaaggag acttgatgat ctctggtgag gatgcgatac aaatggctgg cccagcagtt
     2101 catgaatgga caagaatatt ggaggaatga agatgataaa aggttcgatc gtggcgctga
     2161 tcacgccgat gaatgaagag ggatcagtag attatgctgg tttggaaaaa ttgatccaat
     2221 tccatttaga tgaacaaaca gatggattat tagttttagg tacaacagga gaatcttcta
     2281 cgctcacaca atctgaggag gagcagatcc ttcaactgac agtaaagaaa gtggctggcc
     2341 gtgtgccggt gattgcagga gctggaacga ataatacgaa agaaacgatt gaaaaagcga
     2401 agcattttgc ttctttgggg gcagacgcac ttttggtcat caccccttac tacaacaaaa
     2461 caagtgatgc aggactagct gctcatttta cagcaattgc agaagcctca ccgattccgc
     2521 ttattttgta taatgttcct tctagaacag gcatgtcgat tccaatccat gtattagtaa
     2581 atttagcaga acatcctaat atcattggtt tgaaagaagc ctcaggagat atggcttatg
     2641 tgatggatgc cgctcggttg attggagaag aattcttcct ttacagtgga aatgatgatt
     2701 tgatcctacc agtaatgagt gtaggaggaa gcggtgtaat aagtgtatgg gcgaatatcc
     2761 aacctaaaat agtccatgaa ttagtaaaag atacgcagga cggaagatgg cagcaagcaa
     2821 aagaaaagca attaaatgca cttgaactga tccatgcctt gttcagtgag accaatccta
     2881 ttcctgtcaa agcagccatg tcattattag acttaccttc cggacctcta cgcctgcctt
     2941 tagtttctct ttcaaaagag aaaaagaaac aattagctca gcttttgtta aaagaaagga
     3001 cggttgaaaa atgaaaatcg gactgattgg cagtggacag atgggaaata gagtcgaaga
     3061 agttgcacaa gaaagaggag atacggtcct tcttttacgg acggcaccta aagaagcaac
     3121 tacgcttgct tttacggaac tgccagaact gcttattgat ttttctcatc cagataattt
     3181 agagaaaatc ctttcgtata gcttatcata tcaacttccg ttagtcattg gcacgacagg
     3241 ttatacacaa tcacagttcc aagaaataaa agaacatgcc aaacatgtac ctgtgatgta
     3301 tagtgctaat ttttcgttcg gcatcatggt gatgaatcaa ctaatcaagc aagcagctgc
     3361 aatgttgcaa ggctggcaga tcgaactgat tgaaaaacat catagtcgta aaaaagatgc
     3421 accatctggt actgcaaata tgctgcttca aacaatccaa gaagtgcaaa agctgcagcc
     3481 cgtatacaac tggcaaggga aaaaaagaga agaaaaccaa attggtgtac acagtattcg
     3541 tgcaggttct ttacctggtg aacatgatgt attgtttgct gctactgatg aaatatttac
     3601 gatcaaacat gaatcttttt ctaatcgaat ctttgctgca ggcgcagtag aagcagcgga
     3661 ctggttgaaa gatcagtcac caggttatta caaattagaa gatttattaa tggacaaagg
     3721 ggtgtaaaaa acgtggaagc tcaagaaatc atccgctaca ttcaaacggc atctaaaaag
     3781 acgcctgtga aagtctatct gaatgtaacc gagccaatcg aatttaaaga aagtaaagtt
     3841 tttggtgaag gaagcagctt cgttgttttt ggtgactatg agacgattgc tccggtttta
     3901 gaagctgaat cagaaaaaat cattgaaatc gaaatcgaaa ctgctgcaag atacagcgca
     3961 gttcctttgc tagatattaa aaaaattaat gctcgtatcg aaccaggtgc tattatcaga
     4021 gatcaagtgt caatcggcaa caatgccgtg atcatgatgg gagcgattat caatattggt
     4081 gcagtaatcg gtgaaaacac gatgattgat atgggtgctg tcttaggtgg acgagcgaca
     4141 gtgggcaaaa actgccatat tggtgcagga gctgtcttag caggtgtgat cgaaccagca
     4201 tcggccaaac cggttatcgt agaagacggc gtactggtag gtgcgaatgc agtgattgtt
     4261 gaaggtgtcc atatcggaaa agatgcagtc gtggcagcag gagcagtggt tttagaagat
     4321 gtggcagcag aaactgttgt cggtggaata cctgcacgtg tattaaaaat aagtgatgat
     4381 cagacaaaag aaaatacagc attgattgct gcgttacgcg aattatagag gagatggaga
     4441 aaaatgcgcg aaatagatac ctgtattgtg ggggcaaccg gattagtcgg ccaaacgatg
     4501 ctcaaagtgt tagaagaata tgattttcca gtaggacgat tgaaattatt agcttcaaaa
     4561 cgttcagccg gtaaagtttg tgtattcaag aaaaagaaac acactgtaca ggaattgaca
     4621 gaaacttcat ttgaaggata tgatttagcg ctattttctg caggtgcttc tgtatccaaa
     4681 gagtttgcgc caatcgcacg aagtcaggga ctatttgtca tagataattc ctcagcatgg
     4741 cgggaagatt cggatattcc attaatcgtt ccagaggtca atctggaaga ctatacattg
     4801 aatcggctga ttgcaaatcc aaattgttca accattcaag cggtacttcc gcttaaagct
     4861 ttacaagaag cctttggtat ccagcgagtc aattattcga cctatcaagc agtttctggt
     4921 tccgggcaaa aaggcattga agatcttcat cgtacgcaaa atgacgagga accagttttg
     4981 tatccttata atattagcca aacggtgatt ccggaaatcg atcagccttt ggaaaatggg
     5041 tatacaaaag aagaacagaa aatgataaat gagacccgta agattctgca tcagcctgct
     5101 ttgccagtat cagcaacctg cgtacgtgtg ccaatcgaaa atggacatgg agtctctgtg
     5161 gcagttcaac tgcaaaaaga gtttacgacg gaagaaatta gagagtgtct cagccagttt
     5221 ccagggattc aattagaaga tgatctagca gcacataaat atccgacgag cattcttgct
     5281 agaggaacag atatggttta tgttgggagg atcagaaaag acacttcttt agagaacgga
     5341 ttgttgttct atacgactgc cgataacata cgcaaaggag ccgctgcgaa tgccgtacag
     5401 attgctgcag ctttaatgca gaaaatagcg gaggtggcaa gatgagtaat acgctgaaag
     5461 tagcgaaatt cggcggaaca tcgatgtctc atgaagagca attcaaaaaa gtccgtacaa
     5521 ttgttctttc agatcccaat cgccaagtga tcgttgtttc tgctttaggc aaacgtacgt
     5581 cagatgatga caaagtaaca gacttacttt atttgattta tgcttacctt cagcatcatg
     5641 ttgattggac acccctttgg aaaaggattt gtgatcgttt cattcaagta agagatgttc
     5701 ttgccctgca gttacctatt gaagatgaac tagcacgcat acagaaacaa ctggaagacg
     5761 aaaagataac tgaagattat ttagtaagta gaggtgaata tctgactgct caactgatgg
     5821 ctgagtatct tggttaccag tttattgacg caaaagacct gattttcttt gactatgatg
     5881 ggaaaatcga tcaggaaaaa acagcacagg aaatcacaaa agcatttaaa agatatccga
     5941 aaatggttgt tccagggttt tacggggcga accctgcagg aaaagaaaaa ttattgggcc
     6001 gtgggggaag cgacattacc ggcgcgatac tggcgaagat gttgcaagcg gaaagttatg
     6061 agaattggac ggatgtttca ggaatcatga tggctgatcc gcgaatcatc gatcatccta
     6121 aaatcatcga ggaaatctct tttagagaac tgagagagat ggcttatatg ggggctaacg
     6181 ttcttcatga agcagctgtt ttcccagttc aagaggctga tatccccatc caaataaaaa
     6241 ataccaatga tcctttcgct caagggacaa aaatatcgaa tcacgaaata gaaaaggaaa
     6301 atggactgac aggaatagct ggacgcaagg attttctttc catcacttta ttcaaacgtc
     6361 atatgtccga tgaaatcggg tttatctgga aagcaatgag catttttgct aaacatgaga
     6421 tatcaatcga acatattcct tcagggatcg acaatatcgg agtgatcgtc tcagcagaag
     6481 cgattgccga taagttattt ctcatcacga aagaattaaa agaaacactt ggcgtggaag
     6541 aaatcgtcgt gatcgaagac ttagctttga tctctgtcgt tgggggtccg cataaagaat
     6601 tgattggatt atctgggaaa gtgttatcga tattgaacaa attagaaatt cgcacatcga
     6661 tcttatcaca aggagcacag gagttgaatc tgatcatcgg tgtaccgaat aatcaatatg
     6721 aaacagtagt gaagggtatc tatgaaggga tggtgaatac ggatgctgaa agaatatcga
     6781 atggcactcc atcaaattcc tgaaatcggg tttcatgaat tcaagacaaa aaaatattta
     6841 tatgaacaag taaaaaacag cgaggccgtt atccatgaaa tcggcgaaac aggactactc
     6901 ctttattttg ataatcagca agaagaaacg attgcttttc gaacagatat cgatgcactg
     6961 ccagtcaaag aagaaacgaa tctctctttt gcttctaagc atgaaggtta tatgcatgca
     7021 tgtggtcatg acggacatat ggcgatgctt ttagggttga ctgactttat aactaaacac
     7081 attgaggatc ttatttataa tgtagtactg atctatcagc catcagaaga aatttccggt
     7141 ggcgcacaaa gcgttattca gtcaggattg ttggataaat atgaagtaca agcaatattt
     7201 ggctttcatc tatggcctgg actagctaag ggagaagttt tctctcgacc aggacctttg
     7261 atggcacaaa gcagtgaaac agacattatc attaaaggcc agtctgcaca tattgcttct
     7321 agtgaaaaag gaatcgatag tctggaggca gctgtacgat ttttgaagaa tgtttatgat
     7381 tttgaccggc aattaccggc agagtggaat catctaatga aatttggcca aatccaaggt
     7441 ggaacgatcc gaaatgtttt agctggagaa gttattgtat ccggaagtat ccgttcttac
     7501 agttcagata tgcaaaatta tttgaagagg ggattagacc atttagcaga acagtttcac
     7561 tctacagcta ctatttcttt tcgctataat gacggctatc cagctgtaat caaccataga
     7621 ggcttatatg cagcactgtt agattcaaac aaactacatg aattagatgc tccagttctt
     7681 caagccgaag attttggcat atatacgcaa caatatccat gtgtattttt ctttttaggt
     7741 gtcggtgata cccctgcttt acatgatccg acgttcgatt ttgatatgga tgttttagaa
     7801 aatggtgtag aatggtacaa aacgattctt tttacagaaa atcttcttgt aaaaatcgat
     7861 agcagttcaa aagaggagcg agtaaataat gatgaattta tccaatcgat ttagtcctag
     7921 gctttctaga atagaagtat caaaaatacg acaattcgat cagcaaatat cgtcgatacc
     7981 agatgtgatc aaactcaccc tgggagaacc ggattttcca actcctgaac atgtcaagca
     8041 agcaggaata gcagcgattg aagaagactt ttctcactat accggaatga gaggattaga
     8101 ggaactgcgg gaagcggctt gtatatttca gcaacagcgt tatggattaa cttatgatcc
     8161 gcaaacggaa gttttgacga cagttggtgc aacggaagcg attgcctctg cgttgttatc
     8221 tgttttggaa gaaggagaca aggttcttat tccagcgcct gcttattcag gatatcagcc
     8281 attagtggaa ctagctggtg ctgaattgat cccgatagat acttctgaca cagggtttgt
     8341 ctgtcagccg gaacaatttg aacgagcatt tgaacaatat ggatcagctg tcaaagcagt
     8401 cattttaaac tatcctaaca atcctactgg gacgacattg tctgcaaaac aattgaaagc
     8461 aatcgctgag gttttaaaaa aatatccggt atttgttata agtgatgaag tatatgctga
     8521 attgacttat agtggaacgc atatgtccat agctacttat cttccagaac aaacaatcgt
     8581 gataagcggg ttatccaagt cacatgcgat gactggttgg cgagtgggct ttatttttgc
     8641 tcaaaaagcc cttattgacg aactaattaa agtccatcaa tatctggtga ctgccgctac
     8701 aacgatgtcg caaaaagctg cggctgaagc gctaataaat ggggtgaaag atagcgagaa
     8761 tatgaaagag caatatcaaa cacgaagaga ctatctggtc cagcaattgg cgcctttagg
     8821 atttgaagtg acccagccaa acggtgcatt ttacttgttt tgcaaactcc cagctgccat
     8881 tcaaactgac tcttggcagt tttgtgtaga attagcggaa caggcaaaag ttgcgtgtat
     8941 cccaggaatt gcatttggcc cagaaggaga aggttatatc cggatttctt atgctagcag
     9001 catggaaaaa ttacatgagg cctgcaaacg gatcaaagac tttttaacag aacagtgatt
     9061 catagatttt atcaaaaaaa caatcgttaa ttttgcggta tattctgcaa ggagacgatt
     9121 gttttttaaa tttagttcaa atgaacgcgt ttcttttgat tcataactaa tgtaatgtta
     9181 agtttaaatt gtttagaatg attttaaaag aatattcaag gaggtttcat caatggaaaa
     9241 aatcaaagtt gtcattgtag gtgcatctca tggagggcat caatcgatct tggaactatt
     9301 gacacgatac ggtgaaaatg tagatatcac gctatttgaa gcaggagact atatttcctt
     9361 catgtcatgt ggtatggagc tgtatttaga agacaaggta acagatgtaa atgatgtacg
     9421 taatttcaga ccagaaaact ttccgcaatc caatgttcat attttaaata atcacgaagt
     9481 aaaaacgatc gatgccgata aaaaaacagt aacggttact agcgcatcag ataacgaaac
     9541 aaaagaatat ccttatgaca aattgatttt gagttctggt gtcaaaccaa acaatctgcc
     9601 agtaccagga gcagatctag aaaatgtcta cttgatgcgt ggatacaatt gggcaaccaa
     9661 aatcaaagaa aaattaaatg atccggctat taaaaaaatc gctgtcgtag gtgcgggtta
     9721 cattgggatc gaagcagcag aagcaagccg aaaagctggg aaagaagtcg tattgcttga
     9781 tgtgatcgat agacctttag gcacttattt agatgctgag atgacagata ttttagaaca
     9841 acacctaaat gaaaacggta ttgaagtttt gacaaatgca aaaatcgaag cttttactgg
     9901 taacggaaaa gttgaggcaa tcaaaacaag tgaaaaagaa atcccagcag atctagtcat
     9961 tcaagctgcc ggtgtgaagg caaatacaga atggttaaaa gggatcgtcg acttagatga
    10021 acgtggctgg atccaaacaa atgaatactt gcaaacgaat cttccagatg tgtatgcagt
    10081 aggagatgct acacttgctt attctatccc agcacgcaaa aaaatgccga tcgctttagc
    10141 gactgttgca cgtagagaag cacgttatgt agtaaaacat ctttttgaag aagtaccaaa
    10201 caaaccgttc ggcggagtcg taggttcctc tgcattaagt gtctttgact atcattttgc
    10261 agcaagcgga ttgaatagct ttacagcagc aaaagcagga gtagatgtac gagtgtctta
    10321 ttatgaagat acgttacgac caaaatatgt accagaagaa caaggaaatc caaaagtgtc
    10381 catccaatta ttctcggatc caatgaccca tcaattgcta ggaggagcag tattatctac
    10441 ctatgatgtt acaggtcaag gaaatgtatt ggcactagcg atccaaaaca aactgacttt
    10501 agaagaacta gcagaagctg acttcttctt ccagccaggc tttgaccgac aatggagcat
    10561 gttgaatcta gccgctcaac aagcgttagg agaagaacca tttgtagaat aagaaaacga
    10621 aatatttgaa aagaggttgt gaaaaagctg ccttgcatca agtaataaga acagccaagt
    10681 aaaaacagct tcttatgttt ttaacttggc tggacttatt accgcagatg caagcttttg
    10741 aacaccgttt attttcaaaa aagggactgt gacacgattt gtgtaccgac ccccaaaagt
    10801 tagaccaaaa aatctaactt ttggaggtcg gtatttttta tggttaaata tacttttgaa
    10861 cttaaattaa agatcgttca tgattatttg gatggcaaag gcgattggac cgtaaaaatc
    10921 cagatcaaga gattgaagcg gaaatgctta aaattcggga gaagcacaag gattatggct
    10981 gtttacggat gaccaatgaa ctaagaaacc gcggtttctc tatcaataaa aagaaagttc
    11041 agcgcttgat caagaagcta ggaataaaag taacggcata cacgcgtaaa tcacgtcgct
    11101 acaattctta tcgaggaaaa gt
//
LOCUS       DAZKOB010000091        10581 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000091 DAZKOB010000000
VERSION     DAZKOB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.093"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(283..768)
                     /locus_tag="ACG8M6_002275"
     CDS             complement(283..768)
                     /locus_tag="ACG8M6_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0457896.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    858..1203
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1248..1889
                     /locus_tag="ACG8M6_002276"
     CDS             1248..1889
                     /locus_tag="ACG8M6_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0457897.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1908..2543
                     /locus_tag="ACG8M6_002277"
     CDS             1908..2543
                     /locus_tag="ACG8M6_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0457898.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2561..3376
                     /locus_tag="ACG8M6_002278"
     CDS             2561..3376
                     /locus_tag="ACG8M6_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0457899.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3695..5131
                     /locus_tag="ACG8M6_002279"
     CDS             3695..5131
                     /locus_tag="ACG8M6_002279"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0457900.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5320..7767
                     /locus_tag="ACG8M6_002280"
     CDS             5320..7767
                     /locus_tag="ACG8M6_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0457901.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7997..9064
                     /gene="rlmN"
                     /locus_tag="ACG8M6_002281"
     CDS             7997..9064
                     /gene="rlmN"
                     /locus_tag="ACG8M6_002281"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0457902.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9054..9260
                     /locus_tag="ACG8M6_002282"
     CDS             9054..9260
                     /locus_tag="ACG8M6_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457903.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9257..10360)
                     /locus_tag="ACG8M6_002283"
     CDS             complement(9257..10360)
                     /locus_tag="ACG8M6_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0457904.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3503 a         1803 c         2291 g         2984 t
ORIGIN      
        1 aaaaagttta gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa
       61 attccgattt ataatcgaaa aaagaagcat ttttatatat gcttcttttt tcgtagaatc
      121 aataaatatg gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac
      181 tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag
      241 aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac
      301 tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt
      361 agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat
      421 cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc
      481 tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt
      541 taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata
      601 gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga
      661 aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc
      721 cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc
      781 tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat
      841 tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga
      901 gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt
      961 ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat
     1021 gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt
     1081 aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt
     1141 gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc
     1201 ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga
     1261 ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta
     1321 ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta
     1381 agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt
     1441 tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg
     1501 aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg
     1561 gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc
     1621 cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg
     1681 tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag
     1741 gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc
     1801 taggcgtggc aatcatctat cttacattga ctggtattct aacaatcata atgaaacaag
     1861 ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg attgaattga
     1921 agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc
     1981 cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac
     2041 gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt
     2101 tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc
     2161 agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga
     2221 aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta
     2281 gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcta
     2341 ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt
     2401 tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg
     2461 taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac
     2521 taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct
     2581 attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc
     2641 agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt
     2701 tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc
     2761 caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat
     2821 gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac
     2881 gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct
     2941 gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg
     3001 tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga
     3061 gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct
     3121 gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa
     3181 acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt
     3241 tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga
     3301 tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt
     3361 tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc
     3421 cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca
     3481 acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa
     3541 aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc gactttttca
     3601 caacctcctt tacataattt tattttgttg tggaaggggt ttcgtgttat tctaaaagag
     3661 gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt
     3721 tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca
     3781 agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt
     3841 taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc
     3901 tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt
     3961 tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga
     4021 cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca
     4081 agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag atttcactca
     4141 ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa
     4201 tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac
     4261 agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat
     4321 caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc
     4381 aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct
     4441 gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag
     4501 gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga
     4561 atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa
     4621 tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga
     4681 agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa
     4741 ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg
     4801 ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga
     4861 agataaacaa atccatgacg actatcgaat caattactta caaagccacg tcaaagccat
     4921 caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga
     4981 tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga
     5041 cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa
     5101 actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc
     5161 tggatatggc cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat
     5221 aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt
     5281 tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct
     5341 actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg
     5401 ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta
     5461 ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa
     5521 atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt
     5581 tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta
     5641 ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg
     5701 acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt
     5761 gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt
     5821 tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc
     5881 tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt
     5941 gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca
     6001 gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaaaaaga agctgcagaa
     6061 tatgagaaga aaaaagcggt gagccagcaa agggaaaata ctgctgatga atggcaggaa
     6121 ccagaaaata cagagccgga gcagctaagt tttgttccaa tcgacagttt tcaagaaaat
     6181 atccatcctg cgaatctaga gaaaccagta ccagatacac caaaacagac caatacagca
     6241 gaaggttttt cagatgagtt gccagaggat gacggtacct cattagaatt tgagattgaa
     6301 gcagaacagg aaaatcaaga ctatgaatta ccgacagttg atttgcttga ttctatacca
     6361 actgtggatc aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt attagaacaa
     6421 actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg accatccgtc
     6481 acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca
     6541 gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc gattccaggt
     6601 aaatcactga ttggtattga agtaccaaat ggaaagatca gtatggtttc ctttagagaa
     6661 atcatcgaag cacaaccaaa ccacccagat aaattactag aagtaccttt aggacgtgat
     6721 gtttctggac gggtacaaac ggctgatctt tcgaagatgc ctcacttact cgtggctggt
     6781 tcgactggta gtgggaagtc agtagcaatt aatgggatta tcacaagtat attaatgcgt
     6841 gctaagcccc atgaagtaaa actgatgatg atcgatccga aaatggttga attgaatatg
     6901 tacaacggca tacctcatct gttgacgccg gtcgtgacga accctagaaa agcagcgcaa
     6961 gccttacaaa aagtagtaca agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga
     7021 gtaagaaata tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg
     7081 aaacatccaa ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg
     7141 gtagccagca atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg
     7201 gggattcata tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc
     7261 aaagcaaatg taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg
     7321 atcattgatt ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca
     7381 atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa
     7441 cgggtcgttc aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg
     7501 acagatgaag ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa
     7561 gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt
     7621 cggatcggtt acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt
     7681 ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa
     7741 acgttgccgg acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa
     7801 ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc
     7861 taatacggta tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta
     7921 tttgtgaagt attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa
     7981 aaagagggaa aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat
     8041 caattggttt atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt
     8101 gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga
     8161 aaaattatcg gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc
     8221 ggatggtaca gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact
     8281 gatgagacaa gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg
     8341 ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat
     8401 tgttgcacag atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt
     8461 ttcacatgtc gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga
     8521 ctttttgcat atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt
     8581 ttccactagt ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa
     8641 tctggctatt tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa
     8701 ccgaagtttc ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa
     8761 ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc
     8821 gcaacaatta gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc
     8881 atataatcca gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag
     8941 attttatgat gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac
     9001 agatattgat gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa
     9061 ataatcgtat cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg
     9121 aactgttcaa cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga
     9181 caagaaaaac cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg
     9241 gggttttagc aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat
     9301 gataagtttc tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca
     9361 catgtttaat atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga
     9421 cattgagatt catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga
     9481 ttttatcaaa caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga
     9541 cagatgctcc gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg
     9601 agtgtgccat agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa
     9661 caatgatatc cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt
     9721 tcaatgcttg cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc
     9781 ctaatctatc cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt
     9841 tggataaagc atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg
     9901 ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc
     9961 cttcttcgtt atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg
    10021 gaaaggtgaa gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac
    10081 ggccaccacc gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact
    10141 gttcaatatt tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt
    10201 cctgtgtcaa agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa
    10261 tcagcactac ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg
    10321 ttccaatggt ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc
    10381 gttgaaacgg gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag
    10441 aggtgaacct aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca
    10501 ataagtatcc taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaatttttg
    10561 ttcctcttag tatttctacc g
//
LOCUS       DAZKOB010000092        10381 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000092 DAZKOB010000000
VERSION     DAZKOB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.094"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..253
                     /locus_tag="ACG8M6_002284"
     CDS             35..253
                     /locus_tag="ACG8M6_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457905.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(542..1054)
                     /locus_tag="ACG8M6_002285"
     CDS             complement(542..1054)
                     /locus_tag="ACG8M6_002285"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0457906.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1068..3365)
                     /gene="recJ"
                     /locus_tag="ACG8M6_002286"
     CDS             complement(1068..3365)
                     /gene="recJ"
                     /locus_tag="ACG8M6_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0457907.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3507..3950)
                     /locus_tag="ACG8M6_002287"
     CDS             complement(3507..3950)
                     /locus_tag="ACG8M6_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0457908.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3951..4739)
                     /locus_tag="ACG8M6_002288"
     CDS             complement(3951..4739)
                     /locus_tag="ACG8M6_002288"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0457909.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4736..5677)
                     /gene="rnz"
                     /locus_tag="ACG8M6_002289"
     CDS             complement(4736..5677)
                     /gene="rnz"
                     /locus_tag="ACG8M6_002289"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0457910.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5854..6441)
                     /locus_tag="ACG8M6_002290"
     CDS             complement(5854..6441)
                     /locus_tag="ACG8M6_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0457911.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6662..7975)
                     /gene="obgE"
                     /locus_tag="ACG8M6_002291"
     CDS             complement(6662..7975)
                     /gene="obgE"
                     /locus_tag="ACG8M6_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0457912.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8263..9273
                     /gene="gap"
                     /locus_tag="ACG8M6_002292"
     CDS             8263..9273
                     /gene="gap"
                     /locus_tag="ACG8M6_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0457913.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9358..10293)
                     /locus_tag="ACG8M6_002293"
     CDS             complement(9358..10293)
                     /locus_tag="ACG8M6_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0457914.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2867 a         2343 c         1806 g         3365 t
ORIGIN      
        1 caattgttcg cataaattaa agaggtgaga attcatgaaa tcaatgagac gtcttggctt
       61 agtattaact gttttgtttt taggtattat tattggtaat ttcggcatac gtccagcttt
      121 acttgtgttt gttccgttat ttactttgtg gttcatgttg tgggatgaaa agaagtaccg
      181 tcaatctgag cggcgcagaa ttcatgaaga tcatttctat catgagccct actacagatc
      241 caggcaatca taataaggat tgactgctaa ttattcaaaa gacaagaagt taaactttta
      301 cgggggtgtg acaaaaatcg gtcacatcct ttttttcgaa taaacggtgt tctaaagcca
      361 gtttttcggt attaagtcaa attgcggaaa aagtgatttt ttaaacttct actggtcttt
      421 gcttgtttcc atttataaaa aaatagcgac cctcaaaagt cagatagaga atctgacttt
      481 tgagggtcgc tttaaaatcg cttctttaag aataacatca agaatagctt cttttttctg
      541 cttaatattc cattaatgta atgatgtcat atcctttgat tttgtcgcgt ccatggagat
      601 ccatcaattc gatcaaaaat gcgcagccta cgactacacc gcctaattgt tcgatcagtt
      661 cgatcgtcgc tttgattgtt cctccagtag ctagcaaatc gtcacaaatc agcacacgtt
      721 gacctggttt aatggcatcc ttatgcaatg tcaatgtatc tgttccatat tctaatccat
      781 aagtcacttc gatggtttct cgaggtaact tccctttttt gcggactggg gcaaaaccga
      841 ctcctaactc ataagcaaca ggacatccta cgatgaatcc acgggcttct ggcccaacta
      901 ccatatcaat tcgtttttct tttgcataat ttacgatttg tttagtagct tcccgataag
      961 cgtcaccatc tgccatcagt ggggaaatat ctcgaaacac aattcctttt gacggatagt
     1021 cgggaatact ggcaatataa tcttttaaat ccactctttg tttcctccta ggttgttaac
     1081 catttcttta tggtaggaat atcgctcaaa agcaaaaatt cctcaacttt gattttattg
     1141 agccgtcgct ggtataatct gctctcagtc aaaggatgac tttctgggct agcgactttt
     1201 tgtaaaacac cgttctctat tgtaacaaat ttcaattcaa aaaacacctg tatcataaaa
     1261 attaataatt tttcagggat acgcaaatat tgagctattg cttttatttt atgacgtata
     1321 tcaattcgtt cttgagaatg gatcaattta aacaattttg cgtactgttc tcttgtgcct
     1381 accccattca aataggcctc atcaggtgaa atccccatca gataaacacg attgaatgaa
     1441 taagaccgaa aaacttcttt cagatccgct aaatcatctg gacaatccat aacaactaga
     1501 gagtttggtt ttcttttttc aattattgcc tttacagaat ctagtccttc gaaaacaatc
     1561 gcagcttcct ggataatctc aggcacgaat ttcaacgtcg aagaatcaaa tgctaaataa
     1621 aggctacctt ccgacgttcg gctctgttca cggaaacgtt ttgcccgcca atcaaatact
     1681 tgcagaccac ccacagaaaa atcagatacc atcagctgcg gtttcttacg cccgttccat
     1741 tcattgatcg aaagctttcc taccacatct acctgattat tcagcagttc ttcttcttga
     1801 gtaccaaaac caaaagcgac cgcatcaagc tgactcgatg catcggaaag gacaaatttc
     1861 agatgctgtt ggtttgctcc gattcttttg atattttctg cttggacttg tcgaaataaa
     1921 aactgaggca aaggattatc cgtaccaaat ggcgccaata gtttcagctc atcaattcgc
     1981 tctactgtta cttcatttgg cagcaatact tcatcaattc tcaactcagg ccctctcatc
     2041 aagtcaattt gatgatcgac tatatattgg ttcatcttct cctgtagtat agtgacattt
     2101 tcacttggca tcgttaaacc gactgctgca tgatgtcctc cgaagtaagt aaataaatct
     2161 cgcatctggt caagcatttc aaaaagattc aaggcttcaa tactacgtcc agatccttta
     2221 gctgatccat cttcttttaa agtaaggaca agcgttggtt tacccgtttc attcattatt
     2281 ttcccagcaa caatgcccaa aacgccttca tgccatcctc ggtttaccaa tagatggata
     2341 tgattctcct tattgaccat cgctaatgct tcttgtgtta tctgctctac gattgctttt
     2401 ctttcttcat tgatctcatt caattttttt gcttgaacgg atgcttcctc ttcatcaaac
     2461 gtcgcaagta acgagacggc aggacgtgga tcgcccatcc gaccgattgc atttagtctg
     2521 ggagcaatcg aaaaaccgat cgttgtttcg tctgcgtccc tcatttttac gccgctttct
     2581 tcaaatagcg cctgaagtcc tatccgttct gaatgatgaa tggcatcgat tcctaatgaa
     2641 acaaggattc gattctcatc agtcaatgaa actaaatcgg cgatcgttcc tatcgctact
     2701 aaatccaaaa attctgcagg cggttcttcc aataatgcag aagctacttt gaatgctact
     2761 ccgactcctg ccaaatctgg aaatggatag tgccccgctg gatgacgagg gtgaacaatc
     2821 gcataagcgt ctggtaaatt ctcaggtagt tcgtgatgat ctgtaatgat cacgtctaca
     2881 ccagcttgct gagcgtatgc taccgcatca tttcctgcta ccccgttatc aacagtgatg
     2941 atcaactgga ttccttcttc gatcttttct tggtataccg attggtttgg tccataccca
     3001 tgctcaaatc gatttggcag gaatacttct acttctgcac caagtaattc cagtgtttct
     3061 ttcattaccg ttgcactggt gatcccatct gcgtcataat caccgtaaac gaggattttt
     3121 tctccactga ttacagcttc ttggattcgg ctgattgctt tatccatatc gtgtatcaga
     3181 tacggatcat gtaattcttg ttcctttgga tgtaaaaatc ggttgatatc cttttcctca
     3241 tggtaacctc tttgccacag taacttaccg acaaatggag ataatttcag ttcacggatc
     3301 atttggagaa aggattcaga aggctctgtc tcgcttaact tccaatcaaa atttgcttgt
     3361 ctcactttct cactcctaac ttagttatta tagcaaagga aatcagcaaa aaaaagcaga
     3421 aagaaagaat aaaaacagat cagatccgct agacagatgt tcagcaggac ctgatctttt
     3481 gtacgaccat ttatgaaata tccctattag tcaaaataat tgaagctttg tttgggcgat
     3541 tgagtttttt gttcttccgg cgctttcaat tgactgattt gtgcggtttt cgcttgtaac
     3601 tccgcctgat aagcagcttc caactccgca cgttcgtttc taaactcgcg ttgctgttct
     3661 tcttttgctt cttcaatttt cttttgaagt tctgtttgat attctgacat ttcctgagtc
     3721 agctgtttga tttgcttttt ttgctgccat agtgttgccg aagcagtcaa aaatacaatc
     3781 agtgcaccaa tcagcgcaga accgatgatc actaagatca atggcgcaga aaaggaagtg
     3841 aagccaaaac ttacaggaac atttttattg ttcaatacag caaaaagaac aataatcaaa
     3901 actaaaacaa atccaagaat cacattgctt tgctttttca attccttcac ctactttttg
     3961 ttaaaaattc cgctggctaa ataatcacca atcgttggaa acagcgtata aaattttgat
     4021 gcaacttcta aaataaacgg ttgattgatt tcccgttttg gatgtctcat attgcggacg
     4081 atcatttttg ccaacttggc aggttctaaa acgatttgtc cgactttttc caaatagctt
     4141 ccgcttggat cagccttatc gaagaaattt gtctcgattg gacctggatt cactgtcgta
     4201 acagctacgc caagtggttt caattctaat cgcaaagcgt tggagaagcc aaggactgca
     4261 aatttcgtgg cagaataaac cgttgatttt gcagttgcca tttttccagc catagaggcg
     4321 atattaatga tatgaccttg tcttctttct gccatatcga tcgcaaattt ttgtgtcaaa
     4381 accatcattc ctaagatgtt gacttcaaac atatcgtatg cttttccaag gtcgaatgta
     4441 aggaagtctt cgaaaatccc aaaacccgca ttgtttacca gaatatcgac gcgcccgact
     4501 tcttccatca ccttttcata taattcgtcc acgctttcag gattgctgac gtctaccttg
     4561 aaagcataag cctctttgcc gctcaagcgt ctgcactcgt ctctgacacc ttctacaaat
     4621 gccattcttc gtgcgcaact tataacgatt gctccttgct ttgctgcttc ataacagatt
     4681 tgtgcgccca gaccgcctga actacctgtt actaaaacaa ctttatcttc taaattcatg
     4741 cctcttcctc ctcgcgaaat ggaatctcaa tgctatcaaa atctttcata atcgtcgtgt
     4801 tttcaaacac ttcttgtgct tctttttcaa gctctaaagc tgcttttcct aaatatctcg
     4861 cactgatatg agtaagtagc agccgttttg cctgcgcttc ttttgcaact tcagcagctt
     4921 gatgagtcgt ggaatggaaa tagttgtgcg ccattcttgc ttcatcttta ttaaatgtgc
     4981 tttcatgtac cagaatatcg ctattttccg caagcacaac ggaattatgt gtttttctcg
     5041 tatcgcctaa aatagtcacg attcgtcctt ttttatctgg acctacgaaa tcttttccat
     5101 tgatcgtccg cccatcttct agttggatcg tttcaccttg cttcagtttg ccgtacagtg
     5161 gtccagctgg aatacctaat tcttttagac gatccacctg caattctcct ttatgatcat
     5221 gttcaaccac acgataacca aaactgtcga tcccatgatt tagttttttt gtataaacag
     5281 aaaattgctg atcagtaaaa atcggggctg tttcatttag ctcaatgaat ttcagcggat
     5341 aacttaaacg cgtttgcgaa atacctaaag agacttttat gtattcctct atcccttttg
     5401 gcccgtaaat ctctaatggc gtatccccac cttgaaatga ccggctgctg atgagcccgg
     5461 gaagtccaaa aatatgatcg ccgtgtaaat gtgtaatgaa aattttaccg atttttctcg
     5521 gacgaatagt cgttcgcaaa atctgcattt gcgtgccttc gccacagtcg aacaaccaaa
     5581 cttcattgcg ctcgtctaaa agtttcaatg ctatacttgt cacatttcta tgttttgcag
     5641 gaacacctgc acctgttcct aaaaattgta attccataat ctacctgact ttctttaagt
     5701 tcctctttaa ttttagggta aaaagaaggg ataatcaacc gtaagtcgag aatgggttca
     5761 aattaaaaat aacgttctaa agcgatagcg ttttctttga aaattactat aaaaacagca
     5821 gcgaacccgt ttgagatcct ctgctgtttt ttattaattt gaagatccag aactttcatt
     5881 gtcgttggaa gtttgcggat gtttgtcctt ccaagtcttt tgtgcagctt ctttcgcatg
     5941 attttcagct tctttcgcat catgcacatc tttttcgaca tcttcataat ccaagcgagt
     6001 aatttctccg cctaattcca tcattaccaa ttgattcagt gggcgattgt cctcttcatc
     6061 agcaatcaag atcagtcctg tagctccttc accgatttta tttcctacaa agtcgaacac
     6121 ggtctgggca tcttttattt ctttggcatc ttgtgtcgca ccgatcattc ctccggcaaa
     6181 ccatcctaat aaaatcccta gcggaccacc taatacacca accaacattc cgattaatcc
     6241 acccgtagaa gatttgttat taccggtaaa gtcgataaaa tcttctattt tgaattgatg
     6301 tgtcccatta ggatcatggc tgacaactgc catctgttct cctttaatcg aacgaacagc
     6361 atgtaacttt ttgatttctg aaaatgcctg atatgcctgg ctttctactt gaaaatttaa
     6421 aatgattact cgtttgctca ttaaaaacgc ccctttcata ccttcttgtt atttatttta
     6481 cctttaattg aaaaataaga aaagaaaagc actctttttt tccaataaaa tagggctgtg
     6541 acaaaagtcg tatcacagct cctttttccg aataaacggc gttcttgaag ctgatcactt
     6601 ctgtcacaac ctcccttaaa tctgaatcta tattttttgg tatttgtttt tatttgcttg
     6661 cctattcgac aaattcaaat tcaaactcac cgatacgtac aaggtcgcca tctttcgcac
     6721 cgcgtgcccg aagagcttca tccacaccca taccacgtaa ttgacgagca aaacgcatca
     6781 ctgtttcatc atgatcgaaa ttcgtcattt ggaataattt ttcgatcttc tcaccagata
     6841 agatccacgt tgcatctgaa tcacgatcga tttggaactc aggaccttct ggattgaatc
     6901 catagtgtac agtttcttct tccagttctt cttcgtacaa tgggaattct ggcgttactt
     6961 ctaacaaatc tgctgtagca ttcaataacg cgtccaatcc ttgtcgagtc actccagaaa
     7021 ttggaaatac tgggatgtca tcagcaaatt catcttcttt ttccttgttc agctgttctt
     7081 tgaatttcac tagattttcc ggtgcatccg gcatatccat tttgtttgcc acgatgattt
     7141 gcggacgctc tagcaagcgt aagttataag tagataattc tttgttgatg gcaagatagt
     7201 cttcataagg atctcgtcct tccatcccac tcatatcgat cacatgtaaa attactcgtg
     7261 ttctttcgat atgacgaagg aattgtgtac ccagcccaac accttgcgaa gctccttcaa
     7321 tcaagcctgg caagtctgct gcggcaaaac ttctgccatc acttgtagta accattccta
     7381 gattaggtac gagcgtcgtg aaatgataag cgccgatttt tggacgagca gatgaaataa
     7441 cggatagtaa agtcgatttc ccgacagatg gaaaacctac aagtccaacg tctgccaata
     7501 ctttcaattc tagttcgatt tttcgctctt gtcctggttc accgttttca gcaatttccg
     7561 gtgcaggatt ccgaggagag gcaaaacgga tattccctcg tccaccgcgt ccaccttttg
     7621 ccacaactag tgtttgacca ttttcaatta aatctcctaa aagagcacca gtctccgcat
     7681 ctcggacagt ggttccttgt ggtactttaa cataggtatc ttctgaacca cgaccgtgca
     7741 tccctttact catcccattt tcacctggct gcgctttgaa atggcgattg aaccgaaaat
     7801 ccatcaatgt acgtaaccct tcatcgacga tcaagatgac atcgccgcca cgaccaccgt
     7861 ctccgccagc cggtccgccg tcaggaacgt atttttcgcg acgaaatgca accattccgt
     7921 ctccgccctt gccagctttc acatcgatcg ttacttgatc taaaaacatg gacataatta
     7981 agtcctccta ttattcattt atttatttga ctgcctgaat ttccacaata aacaaaagtc
     8041 ttacttattt tatcggtgaa atctgatttt gtctatcatt tcagcaaaaa gattgtttac
     8101 cttcttcttt ttttatcatt tggtaaggtt tccaaccttt tttctgctac caagtataat
     8161 tttttgacaa aatattcttt gcgtagcaga ataacagtgt attaattgta ttaattgagg
     8221 atgccgtttc acagcacccc taataaagga ggaacttttg atatgacagt aaaagtaggt
     8281 attaacggat ttgggcgtat tggccgtctg gctttccgcc gcatcaaaga agtctcagat
     8341 gatattgaag tagtagcaat caacgactta acaagtccaa ctatgttagc acaattattg
     8401 caatttgact ctactcatgg tacttatcct ggcacagtta ctgcaacaga agactcaatc
     8461 gtagtggatg gcgaagcaac tcgtgtttat gcagaacccg acgcaagcaa aatcccttgg
     8521 gcaaaagaaa acggtgtaga tatcgtacta gaatgtactg gtttttatac atctgaagaa
     8581 aaagcaaaag cgcatttaga tgctggtgtc aaacgagtag ttatttctgc cccagctggt
     8641 gctatgaaaa ccatcgtcta taacgtcaat gatgatacat tagatgaaaa cgacaaaatc
     8701 atctcagcag gttcatgtac aacaaactgt ttagcaccta tggcttattt cttaaataat
     8761 gaatttggga tcgaagttgg tacaatgaca acagttcatg cctacacttc cacacaaatg
     8821 ctcttagatg gacctgtaaa aggcggtaat ttgcgtgctg ctcgttcagc tgctgacaat
     8881 actatcccac attctactgg tgctgcgaaa gcaatcggct tagttatccc tgagctacaa
     8941 ggaaaattac aaggacacgc acaacgtgtt ccagtcgttg atggttcatt aactgaattg
     9001 gtttcagtat tgaaaacaaa agtaactgct gatcaagtaa acgaagcaat caaaaaacac
     9061 acagttgaca atccttcatt cgggtatgat gaccgtcaaa tcgtttctag cgatgtcatt
     9121 ggaacaactc aaggttcgat ctttgaccca acgcaaacag aagtaacaac tgctggtgat
     9181 ttccaacttg ttaaaactgt tgcatggtat gacaatgagt atggatttac ttgccaaatg
     9241 attcgcttac tagaaaaatt cgctaacttg taatttgata gatcaatcga taaaagaagc
     9301 ctttgactgg atccagtcaa aggcttcttt cttttcatcc cctactttta aactcgttca
     9361 atgaacatat tggcttaaaa attgttggac tacttgctga tatttttcag gatctgtctt
     9421 gtatgcttcc gcatgctcgg ctccttttac aacatacttt tcttttggtc ccttcgttgc
     9481 acgatagacc ttatctaaca tttcaaatgg tacaaatgta tcttcgtctc catgtataaa
     9541 gagcattggg cgctggtttt tcttcaattg ttccactgca tttgcttcac cgaaaaagta
     9601 gccagcacgg acttttgtca ctagactcgt tacttgcata agcggaaatg ctggaagact
     9661 aaacatatcg tctagctgat aagcaagctc atcatgaacg gaagaataac cgcaatcttc
     9721 aatgattgct ttcacattat ctggtagttt ttctccgcta gtcatcataa ctgttgctgc
     9781 tcccatgctg acaccgtata gaacgatttc ttgcgatttt ccattgtttt ctagcacttt
     9841 gtttatccac tgtacatagt ctttacgctc tggccatcca aatccaatgt aatccccttc
     9901 gctttttcca tgtcctcttg catcaggaac aaggacattg taacccatat catgaaacat
     9961 ctttgcgtaa ttggtcatcg tttcagcgtt tcccatatag ccatgagcca caagagctgt
    10021 tttttcactt ttcgtttcag caggaagata aatcgctgat aatttcagac cgtcttttga
    10081 agtgatggtc caatcgcttc ggttttcttt tgaactaaac catttatgtg catccgtttc
    10141 tttggatgtc tgtgttgtac caggtgtatc ttcgtctaaa aagtctttct cagaaggcac
    10201 aacagcataa ttataaaaat aattccctgc aaacactaac gcaacaatca gagctaaagc
    10261 gacgcctaaa aggattttca tccaaagttt cataaatcat ccatctcatt tctctttttt
    10321 catataacct tgaattagta taatcaaaga atctctacta tgcaatgttc atttttataa
    10381 c
//
LOCUS       DAZKOB010000093        10319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000093 DAZKOB010000000
VERSION     DAZKOB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.095"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8M6_002294"
     CDS             6..305
                     /locus_tag="ACG8M6_002294"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0457915.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8M6_002295"
     CDS             378..1739
                     /locus_tag="ACG8M6_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0457916.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8M6_002296"
     CDS             1761..2021
                     /locus_tag="ACG8M6_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457917.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8M6_002297"
     CDS             2035..2787
                     /locus_tag="ACG8M6_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0457918.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8M6_002298"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8M6_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0457919.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8M6_002299"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8M6_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0457920.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8M6_002300"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8M6_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0457921.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8M6_002301"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8M6_002301"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0457922.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8M6_002302"
     CDS             7161..8558
                     /locus_tag="ACG8M6_002302"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0457923.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8M6_002303"
     CDS             8639..9079
                     /locus_tag="ACG8M6_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0457924.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8M6_002304"
     CDS             9227..9553
                     /locus_tag="ACG8M6_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0457925.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8M6_002305"
     CDS             9554..10303
                     /locus_tag="ACG8M6_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0457926.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3269 a         1821 c         2141 g         3088 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgt
//
LOCUS       DAZKOB010000094        10292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000094 DAZKOB010000000
VERSION     DAZKOB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.096"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..251)
                     /gene="acpP"
                     /locus_tag="ACG8M6_002306"
     CDS             complement(9..251)
                     /gene="acpP"
                     /locus_tag="ACG8M6_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0457927.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(373..1374)
                     /gene="plsX"
                     /locus_tag="ACG8M6_002307"
     CDS             complement(373..1374)
                     /gene="plsX"
                     /locus_tag="ACG8M6_002307"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0457928.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1423..3459)
                     /gene="recG"
                     /locus_tag="ACG8M6_002308"
     CDS             complement(1423..3459)
                     /gene="recG"
                     /locus_tag="ACG8M6_002308"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0457929.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3576..5258)
                     /locus_tag="ACG8M6_002309"
     CDS             complement(3576..5258)
                     /locus_tag="ACG8M6_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0457930.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5310..5672)
                     /locus_tag="ACG8M6_002310"
     CDS             complement(5310..5672)
                     /locus_tag="ACG8M6_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0457931.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5942..6130
                     /gene="rpmB"
                     /locus_tag="ACG8M6_002311"
     CDS             5942..6130
                     /gene="rpmB"
                     /locus_tag="ACG8M6_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0457932.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6368..9037
                     /locus_tag="ACG8M6_002312"
     CDS             6368..9037
                     /locus_tag="ACG8M6_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0457933.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(9492..10238)
                     /gene="truA"
                     /locus_tag="ACG8M6_002313"
     CDS             complement(9492..10238)
                     /gene="truA"
                     /locus_tag="ACG8M6_002313"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0457934.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         2800 a         2223 c         1852 g         3417 t
ORIGIN      
        1 atttgaaatt atttaagatg gctgctgata taatctactg cgccaccgac tgtttcaatt
       61 tgttcagcgt cttcatctga aatttccgta ccgaattcat cttcaagctc taaaacgaat
      121 tccataatac taattgaatc agcttttaaa tcatctttga tgttcaattc atccgtcact
      181 ttatcggtat ctatctcaaa atgatttgaa ataattttcg ctactttgtt aaatacttct
      241 tcgcgagtca actttttcac ctccaagaat catacacttg tcaattacct acaccatggc
      301 taattttact ggcatttttc aggaatgtcc accttttttt caatggaacg gatcgcttca
      361 gatatccaaa tactattctt cgcttttttc aaattgttcg actaattgtc caactacatc
      421 tgtctcaagc attgtgtgga tttggcggat cgtatagcga acagcatcgg ggcctgttgc
      481 tccatgagtt ttaatgactg gtgctttcaa gccaaacaaa acggcaccgc catgtttgga
      541 ataatccatt tctgctttca aagaacgtaa gccgtctttt aaaagcatag cgcccatttt
      601 tcctttgatc ccttcattta gaatagcaga ttttaataga ttcatcatat tcattgctgt
      661 tccttcaata gatttcaaaa cagcgttgcc tgtaaatcca tctgtgacca ctacatcagc
      721 cacaccattg agtagctcac gagcttctac gtttccaata aaattcaagc ttgtttcatt
      781 ctgaaggagt tcataggctt ttttcgtcac ttcactacct tttgttgctt ctgttccgtt
      841 gtttaagagt gcgacacgag gacgtccgat acctcggact ttacgtgcat aaaaagaacc
      901 taaaataccg tattgcaaca aatgttctgg tttgttttct gcgttagcgc caagatccag
      961 catatcgaaa ccggcacctt ctttccctac tactggcaag gtagacatca agccagggcg
     1021 ttcgatattt ttgatacgtc ctacgatgaa caaccctgct gctagtagcg cgcctgtatt
     1081 ccctgctgaa aatacagcat ctgcttcacc gtttttcact gcttgtgcag caaggaccat
     1141 tgatgcagtc ttttttcgtc gaattgcttt tactggctca tcatcactat tgattttttc
     1201 gtctgtatgg ataatcgtga tgtttttttc atttgttaag tatttcttga tttctgcttc
     1261 ttttccgtaa agtaagaatt ctacttctgg aaaatcttgc tgcgctaaag ccacgccttc
     1321 tacaattgct tgaggtgcgt gatctccgcc cattgcatct acggcaattt tcattgtttc
     1381 cactcctttt tccgtcatca gtataaagta tagcacagtc tgctaatcga aataacgatt
     1441 atcctgattt tctatttgga ggctttgcgc taaaggctga tactcaggaa gcatctgcca
     1501 atcctttatt tgccaaaggg ctgcagcctc ttctctggct acctccaaaa catttccatc
     1561 cgtcaccaag tcagcaatcg caaattgagg cagaccagat tgacggaagc caaatacttc
     1621 accaggacca cgtaattcca gatctttttc gcttagtaca aaaccattgg tggtctctgt
     1681 catgattttc atccgttcca cacctagttc atttttagga ttagcaacta agatacagta
     1741 ggaagcatca cttccgcgtc caacacgtcc acgaagttgg tgcagctgtg ccaatccaaa
     1801 tcgatccgca tccatgatca gcatgacggt cgcgttcggc acgtttaccc ctacttcaat
     1861 tacagtagtc gaaactaata tctgcatctg gttgtctttg aaggcttcca tgattacttc
     1921 tttttcctga ttcttcattt taccatgtaa caagccaact tcatattttg gtgcaaatag
     1981 ttcacgaagt ttttcataga tttccactgc attttttaca tctaatgctt ctgattcttc
     2041 aatcagcgga cagatgacat acatttgatg tcctcttgcc agttcttttt tcgtccaatc
     2101 cagtacactg tccaattgag gtgttcttac ccagcgtgtt tcgatcggga tacgtccagc
     2161 tggaagttca tcaatgatag atacatccat ctcaccatat gctgtaatag ctaatgttct
     2221 aggaatcgga gtcgctgtca taaataagac atctggtcgc cagccttttt cacgaagtac
     2281 tttccgctgg ttcacgccaa aacggtgctg ctcatccgta ataactagac ctaaattctg
     2341 aaattcgact ccatcttgga tcaatgcatg tgtgccgatc acgacgttaa tttccccttg
     2401 ctctagtttt tcaaggagct cacgtcgttc ttttgctttc gttgaccccg taatcaatgc
     2461 gatggttact tcattgggat caaacagctg ttgcaagctt tccatatgtt gttgtgccaa
     2521 gatttctgta ggcaccatca acgcaccttg gaaacctgct gtaacggtag cgtataacgc
     2581 aatcgcggca accactgttt ttccgctacc tacatctccc tgcaaaagtc gctgcatatg
     2641 ctgcggactg cgaagatccc gacagatttc attcgttaca cgtttttgtg catttgtcag
     2701 ttcaaaaggt agcttttgag taaattgttt caatttttgg acatcataaa ggatctccaa
     2761 accattcgtt tctgcttttt ctgctttttt caatccttga agacgcattt gaaataaaaa
     2821 gaactcttca aaaacgacac gtcgtttggc ttgatggttt tcttcgtggt cttttggaaa
     2881 atgcatactt cttacagcag aagctcggtc aagcagtcga tatttctcca caaggttgtt
     2941 tggcagattt tcttcaatca atgtgccgta ttcttgaaac ccttttttga ttagatcaac
     3001 taatgtcgtt tgacgaattg atttgttgac gtgatatatc ggcgaaaagt catccattgt
     3061 ttgagaacct aaaatcttca tcccgttcaa ggctttacgc tttgcatccc atttaccgta
     3121 tacggcgatt tcctccgata aaatgacttt ttcttttaga taaggttgat taaaaaaaga
     3181 aacgttgaac acatcatgtt cttgcatcat acgaaattgc aatcggcttt ttttataacc
     3241 aaatcgattc atcacaggag gcgagactac gattcctttc aaagtaacct tttcttgatc
     3301 ctggatctcg tttagatttc tttcctgaat atcttcgtag cgaaaaggat aataagtcag
     3361 taagtcttca atcgtttgga tgcccaagct ggcaagacta gctgctcttt tttctccgac
     3421 tcctgccagc atagtgattg gatcatctaa agaacgcata aatttttcct ccctccttta
     3481 acaaaaaaag gacttggtca gcgatcatac ctgatcacta accgaaggcc ttttatgaat
     3541 gaatactgtg ttagaaagat attctgacac agaaattatt cagcagaaaa tagataagga
     3601 tagacaggct gatctccttg gtggatctcg atttccaaat caggatgttt ttctgttagt
     3661 ttctcagcaa attcatttgc ttgttcaact gttccttcat cacctgccaa gatcgttacg
     3721 atctctgtgt cctcattgat catttgttct agtgtactta acgctgttgc tgtgatatct
     3781 gggttagaga tcaggatttt tccatcgatc atgcccaaaa agtctccttc tttgatctct
     3841 acaccgtcaa ttgaggtatc tctgatcgcg tgagtgactt gaccgctcac aacgctagac
     3901 aacatttctg tcatcgtttc tttgttttct tctaaagaag cttgatcatt gaatgaaagc
     3961 atagctgtca atccttggga aatcgttctt gaaggaacaa ctgctactgg gatatctgct
     4021 acttctgctg cttgatcagc tgccatgaag atgttcttat tgtttggcaa gacaatcact
     4081 ttatctgcgt ttacttcttg aatcgcttta acaatgtctt ccgtacttgg attcattgtt
     4141 tgtccaccac tgataacata attagcgcct aaactttcaa acagctcttg gacgccttta
     4201 ccagcagcga tcgctacgat accatacggg atacgtggta tttctttttt cgcaaactcg
     4261 ttcacttctt catcgtgttc caaaatcgtc tcatgctgca agcgcatgtt gtcgactttg
     4321 attttgacta atgaaccgaa tttttgtcca taattcaaga cttctcctgg gttttctgta
     4381 tgcacgtgga ctttgatgat ctcgtcatca ttgaccacaa gtaaagaatc tcctatacca
     4441 tctaaatagt tacggaattc ttcgtaatcg aatgtactat cgactgtagg tccttctccg
     4501 atttttacca tgatttctgt acagtaacca aaatggatat cttctgtcgc catttgacct
     4561 tggacactgc gatgatgttc tgcattgacc atatcatcca tttctgctgg agaaggttca
     4621 tatgtttcat ccgcttggaa ttctccattc aatgcactta aaaagccctc ataaacaaac
     4681 agcagtcctt gccctccact gtctacgaca ccgacttctt ttaggactgg aagcaagtct
     4741 ggagttttag ctaaagcacg ctttgctcct tttacaacag ctgtcattac ttcaacgaca
     4801 tcatcagtac tttttgcttt cttttctcca gctttcgctg cttctctagc tactgtcaaa
     4861 atcgttcctt ctactggttt cattactgct ttatacgcag tttcaactcc atgagtgaag
     4921 gctgcagcaa gatcagaagc atttaatgag acaacatctg gaatttgctt agaaaatcca
     4981 cgaaacaatt gagacaaaat aacgccggaa tttccgcgcg cacccatcag caatcctttt
     5041 gaaagcattg ctgctaattc tcctactttt tctgaggttg aatctactac tgccttagct
     5101 ccactagtca tagatagatt catatttgtt cccgtatcgc catctggcac agggaataca
     5161 tttaatgaat tgacgtattc tgcatttact tgcagacgat tcgcacctgc ctggaccatt
     5221 tcctggaact ggcttgcact tatttctgtt acactcacct agataatcct ccttcagatc
     5281 gactgctgag ttaccagcca gtctcctgcc tagtctggca gtacacgaac accttgtaca
     5341 aagacattaa ctgaattggc agttacgcct aataatgcct ctagattgta tttcactttt
     5401 tcttggacgt tgcgggaaac ttcagagatt ttcgtaccat agctgataat cgtataaaca
     5461 tctacagcaa taccattatc ttcttggcgg acaactactc ctcttgagta attttcttta
     5521 cgcaaaatct cagtaatatt atctttaatt tgatttttgc tggccattcc tacaacaccg
     5581 aacacatctg tcgcggcgcc accaacaact gttgcaatta catcattggt aatttcaatg
     5641 gttccagctg acgtttgaat ttttacagcc atgaatgagc ctccttaacg ggtaaaaaac
     5701 tcccattttt cttctatcat attttatcat agcgcaactt gaaataaaag gcagttaccc
     5761 aaagtactca aaaaccttgc aaaatttcag gtataacctt gtttaattta caattattct
     5821 tctcaattgt caattgtatc tatgaaaatg tacttgcaaa ttcccattct atatgatatg
     5881 ttagtctggt atgagctgaa ttattctatt gctccaaatc aaggcaaagg aggaaattac
     5941 catggcaaaa gtttgttact ttacaggtcg caagacaaaa agtggaaaca accgttcaca
     6001 cgctatgaac tcaacaaaac gtactgttaa acctaactta caaaaagttc gcgttatggt
     6061 cgacggaaaa cctaaaaaag tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga
     6121 acgcgtttaa ttgaatgata cttggaccgt tcagatagtg aacggttttt tatttaataa
     6181 aaatttttaa gggtgggact tttgattcct accctttttt tgcgtttatt tttgttatac
     6241 gaacaaatta tgaagttatt tattctaaat tttatttttt tgtgaaaact agactctttt
     6301 taagcagaga aaaacaagga tatgataaac tgaatacaac ttcatagaaa tttggtggaa
     6361 aataaatatg aaaaaaaatt taaagtcatt ctttagagaa aacggacgct acgcagcagc
     6421 tagtttcctg attccgtttt tgattttagc acttgtttat ctaagtatcg gaatctatcc
     6481 tggaagcagc agaagtattt tggcaagtga tgccttttca cagttttcta attttcatgc
     6541 cagcttccga aatgttcttc ttggaaagca aagtattttt tatacatgga atgcttctct
     6601 aggattgaat tatttatctt taatttctta ctatctaggc gggatcttca cgccactggt
     6661 gttctttttt ccaaaccaga tgatgccaga tgcactgtat gttctgactt tgcttaaggt
     6721 cggctgtgcc ggattggctt tttggtttct tgcacgtacg ttcaagattc ctcgctgggg
     6781 gcatgttgca cttagcgtca gttacgcatt gatctcattt atcactgcac attcggagat
     6841 catcatgtgg ctggatgcat ttttttatct tccattagtg attttgggga ttcatcgtgt
     6901 tatggatctt aaacggccga aacttttatt tatcagttat ttactcttgt ttttatccag
     6961 tttttatatg ggttttatga ttggcgtatt ttctttctta tattttatcg cccgtctatt
     7021 gacaaactgg tcagaatata aaaaaacgat tgttccttat ggtatcactt ctttactggc
     7081 aggcggcgct tcaatgatca ttgtattgcc agcagtgctg gatcttcggt caaatggcga
     7141 ggcactgagt cagatcacta cgttgaaaac agaagcgact tcttatctgg atctaatcat
     7201 gaaaaatatg attggtgttt atgacacgac aaaatacgga tcgattccgt tcgtttacgc
     7261 aggtctgcta ccattagtcc tctgtctgtt ttattttgcc agtcggaaag taccgttgaa
     7321 aaataaattt ttgtacggca gtcttttcgc catacttatc gcaagttttt atcttgttcc
     7381 attgaatttg ttctggcatg gtatgcatgc accgaatatg tttttattcc gttatgccta
     7441 tctgttctct ttcttagtgc tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga
     7501 cgatcgtggg ctgttgttag gaatcatttt ggtcgtagct gctctctttg cggtggcatg
     7561 gggcatcaaa cctgaaggaa gctacaccta tgtgacgatg acttcctttg tattgacagt
     7621 ggttttctta gggatttatg cttttgctgt gggattttat cagcataaaa aactttcatt
     7681 caatcatctg agtatattgt tattattgat catgtctgct gaagcactgg tcaatacgaa
     7741 cagtatgatc catggcattc ttgatgattg gaattatgct tcccgcagtt tatatactga
     7801 tccttatcct gacttgaaag ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta
     7861 ccgcttggaa aatctggatc cagtatctgc aaacgattcg atcaactatg gttacagtgg
     7921 tatcagtttg ttttcttcta taagaaatcg taattcttcc tcttacctgg ataaattagg
     7981 tttccgttca agagggacaa atttgaatat ccgttataac aataatactt tgctgatgga
     8041 tgggtttaca ggaatcaaat ataacatttc taaaaacgaa agcacgttca gtaaatatgg
     8101 cttccgccaa gctggtcaaa gcggcgacta tacactgtat cagaatgtca atgcattgcc
     8161 actagcattt actgcaccgt tgtccgttaa tgatgttgag cagccagtga cagataattt
     8221 gaccagtcag acaaacttga tcaatacatt gtctggtcta aatgaacagt attttacatt
     8281 ctatacacct actttgaaga atcagttgaa tacgacgatc acaaagacaa cgactggcgt
     8341 cacttactca gaaacagcag ataaccagcc aaaagagctc acttgggaag tccatgtgcc
     8401 tgcaaataca caagcttact taagtttgtt cccaaccaat tttgcgcaat tagaaagttc
     8461 tagtgcgacg atcactgtca atggtacatc gcgaaaaacg caaatcaaca tttctggtca
     8521 atactatgat cttggctatt atcctcaaga gacaacagtg acatttactg caagttttta
     8581 tgggacgaaa gaaataagtt tcatggagcc taaagtcgtt ggtctagatg ttgtagccta
     8641 tcaagccgca atgaatcaga tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa
     8701 agcttctggg actgtaaata ccgctgaaga ttccatgttg gtgacaacta ttccttatga
     8761 caaaggatgg aaagctaaaa ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc
     8821 attcttgatg ataaaagttc cagcaggtaa acatactgtc actttcagtt acttgcctga
     8881 aggatttacg ataggtgcaa tcctttttgt cctatgtatt gcattgttta ttctttatgt
     8941 ctggtacctt gaccgcaatc gcccctttta tggcacgatt gatccagcaa tagaagaaca
     9001 accacgaaac acacacagaa gaagacgtag aaaataaaga aacgatggaa cctgttcaga
     9061 ctgtccgcac caagggcaat caaaacaacg ttccatcgtt ttttggttac agaaaaaata
     9121 ggtaacaaaa agatgattca cttatcaacg cctcgtccac aagtcacgat attcatcaaa
     9181 gatgagaagc tgttttttga aattgctctt tgtgactcag agttaaatga caggctcacg
     9241 acctctttct taaacggtgt tcaatcagct gaccttcggt attagctcaa caaacggcaa
     9301 aatatgagga gctattttcc cgtttgtttc tctaatactc aggtcaaaac gctgatttca
     9361 cgacctcttc cttaaacggt gttccagaag tagcttcttc gaaaataagc cgaaattttg
     9421 caaaattttg agaagtaatt ttcgtaaata ccttcttatt tcttgaagct gaccacttct
     9481 atcataacct cttattcata tttcacttca tacaaataaa gtccttcagg gtgtgctgta
     9541 ggtcctgctg cattacgatc ttttttcgca ataatatctg ggatactatc ttcaggcaat
     9601 cggccattgc caattttcaa aagcgttcct actaagatcc gtatcatctt atacaagaag
     9661 ccatttcccc taaatgtaaa caacaattcg tcttttgttt catttacctc catcttagcc
     9721 tcgtagatcg tacgtatttt atcctctatc gagcttcctg atgcacaaaa agaggtgaaa
     9781 tcgtgagtcc ctaataaatc aggaagcgcc cgctgaattt tttccaaatc aagtgggtaa
     9841 ggaaaataac ttgcataatg gcgtcgaaaa ggactgcgtg gtttccctat atccactcga
     9901 aattgatacg tcttttcttt gaccaaatat cttgcgtgga agtcttctga aacaatttct
     9961 acttgtctta cagcaatatc ctctggtgac tgggtatcta gcgcgaaacg cattcgttct
    10021 aacggacgct cctctggata atcaaaatgg attacttgcc cgatcgcatg tactccggca
    10081 tctgtccttc ccgaaccaaa gaccgtgatt tcttttccat ttgccatttt ttgtaatgtt
    10141 ttttctactt cttcttgaac agtacgccca tttggctgtt tttggaatcc attaaaattt
    10201 gttccgtcgt acgcaatgat tgctttgtaa cgtaccatgt ttttctctcc tttgttccta
    10261 aaaggttatc tggaataatg atttttctat aa
//
LOCUS       DAZKOB010000095        10233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000095 DAZKOB010000000
VERSION     DAZKOB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.097"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..565
                     /locus_tag="ACG8M6_002314"
     CDS             92..565
                     /locus_tag="ACG8M6_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0457935.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            574..801
                     /locus_tag="ACG8M6_002315"
     CDS             574..801
                     /locus_tag="ACG8M6_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457936.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1437..1808
                     /locus_tag="ACG8M6_002316"
     CDS             1437..1808
                     /locus_tag="ACG8M6_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457937.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2064..2306)
                     /locus_tag="ACG8M6_002317"
     CDS             complement(2064..2306)
                     /locus_tag="ACG8M6_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0457938.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2338..3240)
                     /locus_tag="ACG8M6_002318"
     CDS             complement(2338..3240)
                     /locus_tag="ACG8M6_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0457939.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3253..3441)
                     /locus_tag="ACG8M6_002319"
     CDS             complement(3253..3441)
                     /locus_tag="ACG8M6_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0457940.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3455..4018)
                     /gene="amaP"
                     /locus_tag="ACG8M6_002320"
     CDS             complement(3455..4018)
                     /gene="amaP"
                     /locus_tag="ACG8M6_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0457941.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4056..4949)
                     /locus_tag="ACG8M6_002321"
     CDS             complement(4056..4949)
                     /locus_tag="ACG8M6_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0457942.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5027..5950)
                     /locus_tag="ACG8M6_002322"
     CDS             complement(5027..5950)
                     /locus_tag="ACG8M6_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0457943.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5999..6349)
                     /locus_tag="ACG8M6_002323"
     CDS             complement(5999..6349)
                     /locus_tag="ACG8M6_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0457944.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6382..7284)
                     /locus_tag="ACG8M6_002324"
     CDS             complement(6382..7284)
                     /locus_tag="ACG8M6_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0457945.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7277..8134)
                     /locus_tag="ACG8M6_002325"
     CDS             complement(7277..8134)
                     /locus_tag="ACG8M6_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0457946.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8468..9277
                     /locus_tag="ACG8M6_002326"
     CDS             8468..9277
                     /locus_tag="ACG8M6_002326"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0457947.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9317..9814
                     /locus_tag="ACG8M6_002327"
     CDS             9317..9814
                     /locus_tag="ACG8M6_002327"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0457948.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         2958 a         1932 c         1759 g         3584 t
ORIGIN      
        1 atatattttt attgtttttc gataaaaaat agttgtttta caataattat tctgctatat
       61 tttagctatc aaagataaaa gagggataaa aatgaaattt aaaacattgc tattaaaatt
      121 tgtcgtttta ggtataagtg ccgtatttat tttgttcggc gtactttttt atactcagat
      181 cgctacaagc gaaaaaggat tcaattttga ttggcagacc acattatttg tttctgtcat
      241 ttactttgcc cttctcctag ggatcgttat tgcttatttc ttattgaaac tattgaaaaa
      301 aatcgaagat aaccaagctt tttccactca aagcttaaac atactaaaaa aaatcgaacg
      361 aaccattttg tttattggtt tcgccttttt tggcttactt cctaaattta tgaaagtagc
      421 ggatttagac gatgctccag gtttgatgtt ggtaggaatc gctgtcgtat tcattccttt
      481 tgcagtatac acattaacaa gcgtgctgga aaaactgctt gaacatgcga ttttacttaa
      541 gacagatcat gatttgacag tgtgaggtgc gacatggcaa ttatcgttaa tttagatgta
      601 gtattagctc aaaaaaaaat gagtgtgact gaattgagtg agaaagtggg cattacgatg
      661 gcaaacatct ctattctaaa aaatggaaaa gcaaaagcca tccgtttttc cactctcgaa
      721 aaaatctgcc aagttttgga gtgtcaacca ggtgatttgt tgacctataa aaatgactcg
      781 aatactatac cagaagaata aaataaagta gaacaaacag taaataaaaa ataatctgaa
      841 tgatagatgt gtgagacttt tagaagttat ttacgtctat tattcagatt ttttatttcg
      901 attgaatacc gttttatcta taaataagat atgtactgac tcatacaaag ggggcctttt
      961 cttctccatg tactatcact tttcctgata caactggttt cctgttacgt tgtagttgga
     1021 tctgctttta gtataatact ttattcattc caccagactg atagcagttt gagcagtcta
     1081 aataatcatg tagtattaca ggccattctg tttcatacct tgaagtgctt aaaacttcag
     1141 ctaatggccc attgtgttaa ctcttactcc attctttttt agtaagctcc catatatact
     1201 gtaaagcctt ctatatatta tgtcatgttg ctttttaaca tttactatgt agttttgata
     1261 aaatgatttg aataacagtg atggacaatt cttttttatt tttaacatta ttttgataat
     1321 aatgattatt tatttaaaaa tacattagat aacatttgtt acgacacgaa aaagcttcac
     1381 aaacaaatat cgttatttta aaatgagggc gaactcacac aattaggagg aatagtatgc
     1441 catttattgt ttactttttt ctttcattac tcgtgatcta tattccatta ccaacatttt
     1501 tcctgctcgt caatcgtcta gcagagcaac atgatacgat tgctgctctt acgaaacttt
     1561 gtttacttct atttgtttgt ctagattact ttacgagcta ttattggcta aaacagacaa
     1621 gagtaaaaaa gagatattat ttcttccttg tctttttcaa tctctttgag tttctgatcc
     1681 attgcttttt acttcatcag tatcatacga tccatttgtg gatcatctgg gttttcaaag
     1741 tggtcgtatt attttgtatg ttcacctttc ctatctcaag aaaatttagg gaggcattgt
     1801 tttattaaag aagcttctct gtatcaataa agacacattt aaggaaaaac ttttaaaaat
     1861 ctaattggga atttgcccgc ataaataaga ggctgtgaca aaagtcttgt cacagcctct
     1921 tattccgtat aaacagtgtt caaaagcttg catcttcgat aatatgccca accaaatgaa
     1981 aaatatgaga agctattttt gtttggctgt tcttattact tgatgaagaa cagctttttc
     2041 acgacctctt tttaaaagtc tttttacagt tttcgagcaa agaatgatac gattagtact
     2101 aaaatgatgg caccaatcaa tgatggcacg attgccattc ctgcaatact tggtccccaa
     2161 gaacctaata atgcttgacc aagtgctgat ccaactaaac ctgctacgac atttgcaata
     2221 atccccatcg aagaaccttt attcgtgatt gctcctgcaa ttgctccgat aattcctccg
     2281 acgataagtg accaaataaa tcccatgttt cgttcctcct aatgttttac ttccttatta
     2341 ctgaacccgt ggttctgtat tttcttttac tttttgtgcg ccgcttccag ctgctgattt
     2401 tactttatct gtttgattcg aagcaaattc accggttttt ttcgctgcgt cagttacttt
     2461 gtcttgcacc gtttcttgat ctttttcaaa ttcttctttt gttttgatgt ccactacgtt
     2521 agcgttgact tcgatcactt ctaaatgtgt catatttttt acttctttgc cgattacttc
     2581 ttgcatttct tcaaagattt tttcgatatc tttgccatat tccacaatga catccatatc
     2641 aactgctact tgttttttac cgacttctgt ttcgatccca gccgttacat tatctgtatt
     2701 cacaagtttt cctgccacat tagagaagaa accaccgttt gcagataata aaccgtctac
     2761 ttgttcaatt gcgattccga tgattttttg cacaacttta tcttcataat tcagctcacc
     2821 gcgcattgaa tcttcctgtt cagaatcatg ctttccttgg ttatttgtat gatcagaacc
     2881 aagttttcct tgtccgtttg tgtggtcaga actaagtttt tcttgtgctt tcgcatggtc
     2941 agaaggatgt ttttgctctt ccacaggttt agaaggatcg attgcatcat gttgttctgg
     3001 aactttagat tctggagttc ctggatgatg aggtgcatgc ttttctgcat catgtttctt
     3061 ctcttctact ccttgttgaa cgttagggac tactggatct ggatgtgcag atactggtgc
     3121 tgttttgttc ttttcatcgt tcacaccatt tgattggttt ggtgtcgttg ggtctggatg
     3181 tgcagacact ggtttttggt tgttcatttt attgttatta tttttgttat tgttattcat
     3241 ggatgtttcc tcctaagatt taaaatagtg atctaagatt ttcgtttgtt tcagataaaa
     3301 accgatagcg acgccgatcg ctgccatgat caagatcaat aaggtcttga aaaagcccac
     3361 ggtgatccat aaaacggcta atatcaatcc aagtacccca aagatgatag ggagcttgta
     3421 tgtcgaaaat agttctttca tttttggtcc ccccctattc tactcgtttg cggtcattgc
     3481 ttcgtttagc tggttcttca aagcctgtgt atttcaccga tactttcact ggctcttttg
     3541 ttcccaatat gtgttgtact tcattttcga tttctttcat caatgttcct gttttaccaa
     3601 tgagtgctga agttcgtttc aactcccctt ttacattgac ggcgatcttg tttttcgttg
     3661 ctttgacatt tacttttggt gaatggatga attcatcttc atgcaagtga gaacgaacca
     3721 ttccttcaat tgctttttta tccaacgtca actccccatc accatgtttc agtagaaagt
     3781 ttccgattgt ttttggataa aacaggaaaa caagtaacgt gataattgtc aaaatagcaa
     3841 atgcaacggc aagccaaaat acatattgtt gcacgtatga attagtgatg gaaaagaatc
     3901 gaaacttctc tagctgaaaa ggcaatggat aggttgcact taatagtatc gcaaacaaaa
     3961 gcacagatag caaaagtaat gttccgatca ctccgatcgt ttttacacct ttattcattt
     4021 tcgcacctcc tcataggggt gaaacgatca acttcttata agttgatcgc ttctccacca
     4081 gttactccgt aaatttgggc tgtaacataa cttgctttat tcgatgctaa gaaaacataa
     4141 actggtgcta attctcctgg ctgacctgca cgttctagta aactttgctg accaaattca
     4201 gggatcgaat cagaaggttg tccttcgtct agttgtaatg gtgtccagat tggaccaggt
     4261 gctacaccat ttacacggat tcctttaggt gcaagctgtt ttgctaaacc gatcgtaaag
     4321 ttagcgattg aagcttttgt tgctgcgtaa tctaataaat gttcgcttgg attgaatgct
     4381 tgaacagaag ttgttgttac gattgctcct cctgctggga tatgcggaac agcttctttc
     4441 actaaagcaa acatagcgat gatgtttatc ataaatgtgt ctttgacttg ctcaagtggt
     4501 aactcagcaa tagactgaca agaaatttgc tgtcctgcgt ttagtaccat cgtatctagc
     4561 ccgccaagtt cactcacggc tttatctatg atttctttag gtgcttgttc gtcacgaaga
     4621 tcaaaaggta gtaagacaat cttacgtcct gcaactttca cgtattttgc tacttcctct
     4681 gcatcttttt cttctcctgg gaagaattga agagcgatat ctgccccttc tctagcaaat
     4741 gcaatcgctg ctgcacgacc gatccctgaa tctccaccag tgattagtac acggcggttt
     4801 tctagttgat tattgccttc ataggattct tctccacaat caggaacagg aatcatattc
     4861 tgctgtaaag ctggtgtatc ctgatcttgt ttaggaaatt tctccgtatg gtacaagttt
     4921 cgcgggtcga ttaattttgg ttctgtcatt gctgtattcc tcctagtttt taaattgtat
     4981 tgccaaaaca aatttctttg tttgatacct gttttaaata gattctttaa tttgtataat
     5041 ctttccggta taaatggtaa cctaaaacta ctgctgctat caaagagcca aagactacga
     5101 gtataaagcc tagagcagaa cttttacccg gtaatcctcc tgtattcatt ccccaaatcc
     5161 ctgcgaacaa cgccggaacg gaaatgatta atgcagctga atctagatat ttcatgatat
     5221 gatttagatt attgtccatc atcccgttga atagatcacc aatactctct aataaatctc
     5281 gatagatctc gatcattttt tctgtttgtc gctgtctcaa atggacatca tagagtagct
     5341 gttcatcgtc taaacgtttg atgaaagttt cagaattcca taattgatcc attgtttccc
     5401 gttgatctct taaggtatga tcgatatata cgatgtctct ttcaagatct gcctgtttat
     5461 acagttcttc attttccgtt gttttacgag ctgctttatc caaagcgtca attcgctttt
     5521 tcatctctct tagttctttc acgtaatggg tgtaaatatc taataatgta tacgtgatga
     5581 tcttttcaaa cgagtttaaa tgatttccat gctttgtcag cagccgatcc acaaattcgg
     5641 tttttctatc gatacaagta atcaagagat tatcggaaag tacaaaagat attggcgtta
     5701 attttttttc gatacgatta tctgactgat ctagattaat cactaccaaa gaaaatggat
     5761 gctctagttt cgtctcataa aggcgctcca tccgagaaac ttcttccgga tattcggttc
     5821 ctacgaaaat atcatcagga agctcgaatt cttcgattgc ttgctcaatc tcttcgggct
     5881 ttgcactatc aagtacgagc caattatatt ttttttcatc tgtttctatt acattctttt
     5941 cttcatccac aaagtagtat ctcaatgtat acctcctttt tggactttct cgaaactatt
     6001 agctgaagaa gcagttcttt aatttgatat tataatcgag tactgcgatt tcattttcta
     6061 atgaaaagct gtcaagctgt ttgttgaagt ccatgttcaa tcgtttaccg aaataatttt
     6121 gctctaattt agatgtatag atgtccaata atggatgctc gattttagtc gcatattctc
     6181 caaattccga aacaaaagga atgatttgaa aaaagctgcc ttttgcaaaa gcacctttgt
     6241 cccttagaaa tggatcggaa tcattgataa gcgcgagtgc gatttttgta ttttccccaa
     6301 ccttttcaat taacgtctct actgcatttt tagacattgt aatttccatt cctactcccc
     6361 ctcttcagtt gttgtcttct attatttaac aactacatct gtttttggtt gaacttttgc
     6421 atattttgga tatttaggga atttagctac acaacggaaa gccgtcatcc ctgcatattt
     6481 ttcgtaatat ttcttcgttg gcccttcaaa atccttgctc cacggtttca catgtcctga
     6541 aaaatggata atgtaaggat tgttgcgagt ttcttcgtat tcacgttcac ctgctgctgt
     6601 tggatgtttt tttgcttttg ccatgatata gccttgtgca ttccaacgcg gatgaagtgg
     6661 taaccaacgg tcatgtaaga tagcatttaa tgcatcttgg tcatggaatc gtaatttttc
     6721 tggattatgt tcgatgaaat ctaatacttt ttgcgtaata ttttcatcta accatttttt
     6781 cacattgatc agcatcaaac ctgagttgaa ataacgcatt gatttagctg aaattttcat
     6841 tttttccagt cgttgatgga acccagcgtc ttcaacagct gctacgattg aatcaccgaa
     6901 gtcaagacgc cataatttag agatatcttg taaagcgatc atgtcgcaat ccatatataa
     6961 gatacgttca acttctgtgc cgcggaacaa ttccggaatc gcaatacgat aataagctgt
     7021 ttctggaata cgatcgctga ttaccacatt tgtaaagaat tctttattga ttttcaagaa
     7081 ttccacatct gagttcattc gtgcgttttt cactgagaag cgtaaaagct gtttactttc
     7141 aaaatcgata tcatcgtcaa tcacatagaa ccgaacaaat ttagacgggt tgcaattatc
     7201 taatactgag acaaataatg cagctaaatg tggtacgaat tttgtgttac aacttgcgac
     7261 tactgctatt tcttttttat tcattgatct cgcctacttt cgttagagta ctgtgtgcta
     7321 atttttttaa gtataaattg gtatacggat gatctttcaa cgtattccaa ggtttatcat
     7381 gtcctgtaaa gtgaacgatc gaaggttttt catttccttc tttatactga cgttcatatt
     7441 tttcatttgg tgctggatga cgttcaaaaa tcagcgaagt ctgcatgttc cattttggat
     7501 gaagttgttc ccaatcttca taaagtacag cattcaaagc atcttggtcg tgatagatga
     7561 ttcgatcgcc attttcactg aggtaatgga tagttttttc tgtaatttct tttttattcc
     7621 attgatcaat gtcaatcacc atcactcctg aattgaagta caagtcatcg gaatcgattc
     7681 ctaatctttc caaagctttc acttggcctg gatcgataat agcgccgatt gtttttccat
     7741 ttaacggctc atcatatagt tttacgatat cgtctagtac taacacatct gaatctaagt
     7801 aaaggacttt cttataatcc tctttagcta ataagtttgg taaagaaatt cttaagtaag
     7861 cggtagttgt gatatggtca ctgactagga agtcttcgta gatatctttt tccactgtca
     7921 ggaattggat acttgcatta cttgaatatt tatttaccgt ttcttccaat ccttctttac
     7981 tatattctga tagaccatcg tcaatcacat aaaattttat gcgtcttgtt ttattgcaat
     8041 tttctaatgc agttgcgatc atcacgctca agtaaggagc ataattttcg tctgaagcag
     8101 ttaccacagg aactacgccg tatctcttct ccatcaaaat cactcctttt tctaatcttt
     8161 ttctaacctg ttctacatct agaatattcc tcatttggtt ttaaagcaaa atatatgctt
     8221 tgttttcatt gaatatttta aaattttttt atcacaaaac gcatcggtaa gcgctaacta
     8281 ttcgtttttc atggcttttt attcaaaggt attgaccaaa aactactata tatagccacc
     8341 tttttcttaa gaatgttttt tgaaaaaatt cataatgaca aagcaagtta gtttcatttt
     8401 tttaaattct catttaatct taaggtaaca ggaaaataga tatgtaaaaa gaaagtagga
     8461 agataaaatg aaactagcag cgattgattt agacggtacc ttgcttgatc gaaatggaaa
     8521 gataccagaa gaaaatatcc aagcacttcg gaaatttgat gaaaatggcg gaatcgtaac
     8581 cgtcgctacc ggaaggaaca gcatttctgc taaagatatt ttttccgaat taggaatcgg
     8641 tggctatttg atttcttcca atggttcact gattgctgaa atgaaggatg gaaaaatggc
     8701 gaaagttctc agacgttcaa aaatagatag cgatacactg agaaaagcct tcttctatgc
     8761 tcaagaagcg aaaatatcca tcattgctag ccgagaaacc caagatgatc agctgacatt
     8821 cgatgaatca aatctagtca aagatgaccc ttattatgaa catttcaatt tacagaacca
     8881 ttcttttgat caaattttaa atatgttgga agatccttct cttagctatc ttaaattagc
     8941 attaacagat aagagtgaag aaaaactaca gaagctacaa agagatctgc aagcagatgg
     9001 tatccaatca gtattctctg atccgcattt cttagaaatc acgcctacag gtatcactaa
     9061 agctcattca cttgtttttc tggcggatta ccttggaatc gaatcgagtg acatcatggc
     9121 ttttggcgat caagaaaacg acattgctat gttgaattac agcgggttga gtattgcgat
     9181 ggggaatgca cagcgacaag taaaagaaat tgcaaaccaa gtaacactga cgaatgaaga
     9241 agctggtgtt gcttatattt taaaaaactt ctattaaaaa gaataaaatg acgatatgaa
     9301 tgtaggagga aagaaaatgt tagttattat tgatatgcaa aatcatattc ttgatccaaa
     9361 cagcgaattt tatattccag agtcagatca attggcagaa cgtattgctc aaagattagc
     9421 taaagcacgc caaaataatg aatatattct cttcacacgg gatataccag ttgaactgaa
     9481 ggacaaagaa gaagaacgag aagatttgca attgatccct cttctttctc cacaagaaaa
     9541 tgaacgagtg attaaaaagt attactttac gattccacct gaaactttag ctgaaatcca
     9601 ggaaacttta tttgaaagca aagaagaaca aaaagagatc gaagtagtcg ggattgaaac
     9661 aaacttgtgt gtactctcta atctgatagg cctgcagagt gcttttcctg aagcagactt
     9721 ttttgttgat ccaatgttag tctccagtag aaaacacgga gaatccgctt tagagctgtt
     9781 gaaagatttc aacgtctcta tcgtcgaatc ataaaaatat ctatcttcat ttacgatatt
     9841 taacacacaa taaaaaaagc acgcagaact gttttagtcc tacgtgcttt ttctatcaaa
     9901 gtagattggc ttttttatga cacagagtga aacggcaggc tcacgacctc tttcttaaat
     9961 ggtgttcaat cagctgaccc tcggtattag cttaacaaac ggcaaaatat gaggaactat
    10021 ttttccgttt gttaagctaa tactcaggtc aaaacgctga tttcacaacc tcttccttaa
    10081 acggtattca aaagcttgca tctgcagtaa taagaacagc caagtaaaaa cataagaagc
    10141 tgtttttact tggctgttct tattacttga tgcagggcag ctttttcaca acctcttatg
    10201 taccttgtag gactcgaacc tacgaccgga cgg
//
LOCUS       DAZKOB010000096        10030 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000096 DAZKOB010000000
VERSION     DAZKOB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.098"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(494..718)
                     /locus_tag="ACG8M6_002328"
     CDS             complement(494..718)
                     /locus_tag="ACG8M6_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457949.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            909..3686
                     /locus_tag="ACG8M6_002329"
     CDS             909..3686
                     /locus_tag="ACG8M6_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0457950.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3767..4186
                     /locus_tag="ACG8M6_002330"
     CDS             3767..4186
                     /locus_tag="ACG8M6_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0457951.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4198..4668
                     /locus_tag="ACG8M6_002331"
     CDS             4198..4668
                     /locus_tag="ACG8M6_002331"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0457952.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4685..5464
                     /locus_tag="ACG8M6_002332"
     CDS             4685..5464
                     /locus_tag="ACG8M6_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0457953.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5451..6272
                     /locus_tag="ACG8M6_002333"
     CDS             5451..6272
                     /locus_tag="ACG8M6_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0457954.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6289..7338
                     /locus_tag="ACG8M6_002334"
     CDS             6289..7338
                     /locus_tag="ACG8M6_002334"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0457955.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7351..8355
                     /locus_tag="ACG8M6_002335"
     CDS             7351..8355
                     /locus_tag="ACG8M6_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0457956.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8988..10007
                     /locus_tag="ACG8M6_002336"
     CDS             8988..10007
                     /locus_tag="ACG8M6_002336"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0457957.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3362 a         1652 c         2094 g         2922 t
ORIGIN      
        1 ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa aaaagtgggt
       61 tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa tatttattcg
      121 tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat ggtttggcgc
      181 agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca aaagctactt
      241 gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg tttctacctt
      301 gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa aaaggatggt
      361 gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc attatgccaa
      421 taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac aggaaagttg
      481 aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc aagataaaga
      541 tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa cgagtttgtt
      601 ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa gatccgatga
      661 tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc gatttcattg
      721 aatagtcata gcctccttat tctttcatat gttgaaattc cagtataaaa actggaagga
      781 actgtacgtt ttttagaata aaaattataa gggataagta ggctgctgtc tataataaga
      841 tatatttttc aattctttat taaagatttt attgacaaaa tgaatccgtt ttctatgatg
      901 gaggaggggt gaataagttg ttgaagaagg aaattttatg ttacttagag aaccagacag
      961 cctttttcga tttagaacat atgaacgagg tctttacagc acaaaggtta gctgatcatt
     1021 ttggggtaaa aagaaacaca gtcagtcatt atttgaacca gttaaatgat gagcaactac
     1081 tagtcaaaat caattctcga ccagtgattt atttccacaa agaagctttt gaaaaacaat
     1141 tttttaagtt acgcacgaat ttttattttt ctatcgatga tctcaaagag gaacagcctt
     1201 ttttcgcgca accaaaagac ttattctctt ttatgatcgg atacgacgcc agtttgaaag
     1261 aaagtatcga acaaatcaaa acagctcttt actatccaga tggcggatta ccacttttga
     1321 ttacaggcga aagtggcaca ggtaaaagtt atttagttaa tttggtctat cagtattgtc
     1381 tgcttcatga tctgctagaa gattctgcac cattcatcac ttttaattgt gcacaatatg
     1441 cagataatcc tgaacttttg actagcaatt tgtttggaca tgttaaggga gcgtacactg
     1501 gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga tggtgggatc ttgtttttag
     1561 acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact gttcacctat cttgatcaag
     1621 gtgtgattta tcgtatggga gaacctaatt tgtcacgccg gatcaaaaca cgtctctttt
     1681 ttgctacgac tgaagagatt actagtcatt tcttaacaac tttcattcga agaataccta
     1741 tccgaatcga actacctgca cttaatcagc gtagtcgtaa tgaacggcta gaactgattt
     1801 actctttttt tctcgaagaa caaagaaaaa tggggagccc tttgtcagtc agtggaccag
     1861 ctttgtcact tttaacaagt cagaaattat ccggaaacat aggagagctg aaaaatatcg
     1921 tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga acagcctgaa atccatgtca
     1981 cgatccatca tttgcaaaaa gaactacttg cgcagcctat tactaaaaat acagcacagc
     2041 aaggaatcta tattacgcaa gaccagacct tagagcaatt aatggagaag caacagcccg
     2101 aacagcagcg aatcgttaaa agttttgagc aaatcctaat tgatttcaag aaaaataact
     2161 gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt gattcaatta tttgattttt
     2221 tgctgtttga gaccagtaga caagaaaaac acgagctgct ggtttattgg acacaatcta
     2281 tccgagagac ctttcgacaa atggaaactg cctatcaaat caaattcgat ggaaacagtg
     2341 tctatgcctt gagttactat ctctatcagc gaagcactat caaatggtta ccggaagaac
     2401 atgatattat ccaactaatc aaagaattag aacaacaagt ttctcagtct tatccatcta
     2461 gttatcacta tgtccaacga attctggaat taagtaagat caaattagat attgaagaca
     2521 caagtatgga tcggatttta ctgacgatct atctaaaaaa agcgaaatgg tcaaaagaaa
     2581 aacgactgcc aaaagcgatg attgctgctc acggttatgc aactgctagc agtatggcaa
     2641 atgtagtaaa tcgtttatta caagaggatc tatttgaatc atttgatatg ccactagatg
     2701 tcacaccaca gcagattgct tcagaaatcc ttgattattg cgaaaacagc gatgtttcaa
     2761 atggattgat catattagta gacatgggat ctctgaaaga aatccatcag ttttttaaaa
     2821 aacagttatc cgtaccgctt ttgattttga ataatgtgac gacaccttta gcaatcacag
     2881 taggggaatg cctgcaaaaa aatgtatcat tagaagagat agcagaagag actgttcgac
     2941 agatccagcc agaatggcga ctgctctatc cagaggaaaa caaacccaaa gcactcatta
     3001 ctacttgctt cactggaatc ggtacggcgg tccacttaag tgaactgcta gaaaaaagct
     3061 tacctacggc ttgtcagttg aaaatcatcc catatgaata tcagcaatta aaagataaga
     3121 aaaacagtga cccattattt tccatatacg aagtcgtagg aatgattggc accacagatc
     3181 ctgatatcac caatatcgac tatctttcat tagaagacct catctcgggg gaaaagatgt
     3241 ctgtactagc tgaatggttg gacactacta tgaatgcttc agagaaggaa atattcaatc
     3301 agcgcatcat tcgtaatttt tcattggaaa aagtactgga ttccgtgact attctagata
     3361 cagagaaggt gatgggagaa atcgatcatt ttatgagaga attagaggtg cagttgaatc
     3421 gaagcatcag caatcccaaa aaattagcgt tgtatgtcca tgtgagctgt ctcattgaac
     3481 gattgatccg acaaatgcca atcgaaacct atcaaggatt agagaaatgg aagcagtgtc
     3541 aaaaagaagg gttatctgct attaaatctg cgtttagtgt cattgaagag cattatagtg
     3601 tcatgatccc caattctgag attgcctata tctatgatat tttgagtgaa gatacagagt
     3661 ttctctcgat agaagaagaa ttttgaacat aagcaagttg ttgccactaa ttttggcatg
     3721 caacttgctt ttttagtatg tgtaagtgaa aagaggagat gaaaaagtgg atagagaaat
     3781 tatcttagct tcccatggaa aatttgcttc agggatatta gattcattag agctgatcta
     3841 tgggaaaaat cattctatta ctgtattcga ttgctatact gatgagaatt ttgatttagc
     3901 ggaaacagta aggcagcttt ttatgaggta caaagataaa gaaatcattg ttctgacgga
     3961 tctttttggc ggaagtgtca acaacgaatt cttacaatat atcaatcggg atcatgtcta
     4021 tctcgttgcc ggattgaatt tgccactatt gattgagcta gtatctcggt tggacgctga
     4081 agtagagaca actgttttga tccagcaggt gctaactgat tcaaaaaaaa tgatccagtt
     4141 ttgtaacgag cggctcaatc agaaaataga agaagacgaa ttttaggagg aagaaaaatg
     4201 attgtattga caagagtcga ccatcggtta ttacacggac aagtagcttt ttcatggaca
     4261 caaacaatag gcgcagactg tattttgatt gctaatgatg atgtacctac gaatgagata
     4321 cgaaagacaa cgatcaaatt agccaagcca caaggagtca aactagtgat caaatcaatt
     4381 gatgactcga ttgccgcgtt gaaaagcggg gtgacagata aatataagtt attcatcgta
     4441 gtggaatcga tcgaggatgc atataagctg gcagaagcgt atccagaaat caaaaccatc
     4501 aacttaggag ggatcaaggc aaaacctggt actcgcagca tcggaaaagc ggtaaatatt
     4561 ttaccagaag aggaggaacg cttgaaacga ttaattgaca aacaaataga agtagaaatc
     4621 cgtcaagtac cagcagataa aaaagtggat gtaagagaag ttttgtaaga aaggaagaac
     4681 ctcaatgatt gtacaagcag tcttattagg aattgttgca tttgtcgcac aatcagaata
     4741 tgcactaggg acatcgttgc tttctcgtcc gattgtgacg gggctgttta caggaatcgt
     4801 gttaggtgat gtaaaaactg ggatcattat gggcgcaact ttagagttag catttatcgg
     4861 atcgttctca gtaggtgctt cgattccacc tgatgttgtg accggtggga ttttagggac
     4921 ggcttttgct attacagctg gagccggtac agaaaccgcg cttttactag gtttgccgat
     4981 tgctacgttg acattgatat tgaaaaatat ttacttaggg cttttgattc caattatgaa
     5041 ccataaagca gacaattatg cagaagaagg aaattataaa ggagtcgagc atatgcactt
     5101 gcttgccggt atcggtcttt ccttgatgct tggtgttgtg gtaatggttt cttatatggt
     5161 gggaagcaat acgatcggaa atctattaaa tatgattcct gacttcgttc aaaaggggtt
     5221 gtcagtagct acagggttga ttccagctct tggttttgcg atgcttgctc gtttgctgat
     5281 caacaaacaa gttgcaccat attttttctt aggttttgct atagcttctt acttagagat
     5341 tcctgtgacc ggagtagcta tttttggtgc gattttagca gttgtggtag tcaatattat
     5401 gaatgtccgt caactaccac ttcaaacaac tgcaggggag gtagaagaag atgaagaatt
     5461 ctgagatcaa aaacagcaaa ataacgaaaa aagagctaaa tcacgtattt tggcgttctt
     5521 ttcaaatgga gttttcttgg aattatgaac gtcagatgaa tatggcttat gtttatgcaa
     5581 tgattccgat tttgaaaaag ttatatcagt cgaaagaaga aatggcagca gctttgaaac
     5641 ggcatctaga gttttttaac acgaccccac atatcgttac actgatttta ggaatcacgg
     5701 ctgcaatgga ggaagaaaat aaggatgatc ctgaatttga tgtgactgcg atcgatagta
     5761 tcaaaacatc tttgatggga cctcttgcag ggttaggaga ttctttcttt tggggaactt
     5821 tgcgattgat tgccacaggt gtgggtacct ctttagccat gcaaggaaat attttaggcc
     5881 ctatcttgtt cattttgatt ttcaatattc cgcacattct gtttcgctat ctagcaacag
     5941 gttggggata tcgcttagga acgggttttt tgaaaaagat ccaggcaaac ggtatgatgg
     6001 aaagtttgac attaggcgcg tctattattg gactgatggt cgttggagca atgactgcca
     6061 cgatgatcac catcaatatt cctttaaaaa tcggttcagg agaaaatgct gtgactgttc
     6121 aaagtatttt tgatgatatc gttccaaata tcttatgtct tggagcattt ggcatagtgt
     6181 tttatctatt gaagaaagaa gtgaaaccat taacgatctt gatcggacta gctttctttg
     6241 gaatttttgg ttcgtggata gggatttttt aaaaaaggag cagagaaaat gtttacgatg
     6301 caagattata tctatgaaga aaaggaagta ctaagtacga tcttgaaaaa gaatgatttt
     6361 tcgacaagag aacatatgaa aaaaacagtg aatttactga tcttagcaac gggttcatct
     6421 tataacgctt gtttagcagc gaagccagca ttggaatctt atggtgatct tactgtcgat
     6481 attcaagaac ctttttattt tttcaattat ggaaaacttt ctccttcaat cgatactgtg
     6541 attgctgtgt cacaaagcgg gaaaagtgct tctactgttg aagcagtaaa aatgatacaa
     6601 aaacaagggt tacctgttgt tgctatcaca aatgacgtgc aaagtccact tgctttagaa
     6661 gctgcacaaa ttattgattt aggtgccggg gtagaatcag taggcttcgt tacaaaaggc
     6721 tattctgcga ctgttttaca gttactactc ttagggatag gaattggaat aagtaaaaat
     6781 aaaatcagca aaaaaataga acaagactac atgcaacagt tgcgcaaaat aatcaaccat
     6841 ttaccagcga ttattcaaaa aacggaagaa ttttttgatg gatatcaaag tttatttcgt
     6901 ttagcacagc gtttcgtcac cattggatat ggtccaaact ggggaacagc taaagaagca
     6961 gagacgaaat tgactgaaac aatccgcgtt ccgtcacaag gatttgagtt agaagcttat
     7021 atgcacggcc catacctgga ggcagatgct tcgcatttgc tattctttat tgaaggggaa
     7081 agtgtaaata aggaacgttc gcaaaagttg caaagatata tgagtcggta cgttggagag
     7141 actctcacca ttaccacaaa aaaagcaaga aacgagaaaa cacttggatt agcaatagaa
     7201 tgtgatgaat atctgtctgt attagcgctt gtcgttccgt ttcaactatt cgcttataaa
     7261 accgcagtag ctaaaggtat tgacttgaac aaaaagattt ttgaagactt tgatacagtc
     7321 ttgaaaagta aattataaga ggtggaaatc atgctgaaat tcaatgaaga agaacaaatc
     7381 aaagcaataa aaggcgcact agctttacgt ccgcaagtag aagaaatcat cgataaactt
     7441 tatgcggaaa aatttgacgc tgtctattat ctaggaatcg gtggaacgta tgcttcctcg
     7501 atgcaagctg tcacttatat gaatgggaaa agcaacttgc ctgtctttgt acagcatgct
     7561 gcagaatatt acactactgg aaacaaaaga ttaacaaaag attctatcgt tattttgtcc
     7621 tctgttaccg gtacaactca agaagtagtg aaagcagtag aacaaatcaa gaaagtagga
     7681 gcgacactga ttggatttat cgataaagcg aacagtaagt tgtctcagct atgcgatttt
     7741 gtggtcactt atccagctcc tggaacagaa caaatcaaat tttttatggc agcagatcga
     7801 ttgatgtatt tgaatggaga attcgaagct tattcagaat actatgaaca gctcgaacgt
     7861 tatttaccaa cagggttagt ggaagcagag aaaaaagcag atgcatttgg cttatcattt
     7921 gcagaaaaac atcgacgtga tagtatgcat tattttattg gagcagggaa tcaatgggga
     7981 gctgtttatt catatgccat gtgttactgg gaagaacaaa gctggcttcc atcgaaatct
     8041 atccatgctg ccgagtttct tcacgggacg ttagagatcg tagaagaaac aaccccagtc
     8101 acattatttt taggcgaaga tgaacagaga gtgttatctg aacgtgtggc taaacttttg
     8161 ccaaaaattt gcagcaatta tacattgata gacacaaaag actatccagt agaaggtatc
     8221 agtgaaaaat atcgcggacg tgttttgtct ttcttattga tgcatgtagt gacacaacgc
     8281 atcgatgctc atgtggagca gttgaactgc catccattag agatacgccg ctactatcgt
     8341 cagtttgatt attaggatag actggataaa aagaatataa aaaataaaga gagcagcgat
     8401 aactgctccc tttatttttt taaaatattg gcatcaatta cgttataaaa cacatttttt
     8461 gcattatcta cagttcaact tataatctgt ttaatgggac atgtgggtgc caaccatttt
     8521 ttgttatgca atagtactat ttagttgttg catggatccg tagtaaaagt ccaagtacaa
     8581 cgagttgcgc cagtttacat agcgttttgt atccagaatg ttgactaaaa caaaagttcc
     8641 tatgtcaacc gtcaacagag acaatctgtc cgttgcagga cctgacagat ttagaaatgt
     8701 tcaatctgtc tcttatgtat tttaaatcaa tactcactag gagaagtttc ctaacctcct
     8761 aatagtaatg ctggtagtaa cattgtcaaa actgcttcct cttaggaaca agcaaagcat
     8821 aacgagtgta aaaataaaat gtagactatt gtaacatatc attgattatt tcaagtaaaa
     8881 acgataaaag aagtaaacaa acacttaaaa acaatggaaa ttagtcccaa ctttaagaaa
     8941 tgtatactat aatggaaatt atcaattatt tatttggagg aatatccatg aaacaaaaaa
     9001 tcgcagttct tggtcctgga tcttggggca ctgctttagc acaaacattg gcagaaaacg
     9061 ggcatgatgt ccgtatttgg ggaaatgtac ctgaacagat tgatgaaatc aataagtatc
     9121 atacaaatca gcatttttta cctgatttga cgatcccaga atcgattatc ggatataaag
     9181 aattggcaga agcagtggat aacgcagatg ctattttatt tgttgttcca acaaaagcga
     9241 ttcgttcagt cgcacaggaa cttattggta aaatgaatac taagccaatc attatacatg
     9301 caagtaaagg actagaacaa gatacgcata aacgaatctc agaagttttg gctgaagaaa
     9361 ttccagaaga aaaaagacag gcaattgttg tattatctgg tcctagccat gctgaggaag
     9421 tggcagtaca tgatatcaca acaattacag ctgcaagtat tgatcaaaaa gcagcagcct
     9481 atgtacaaaa actgttcatg aatgattatt tcaggattta tacaaataat gatgtgattg
     9541 gcgtagaaac tggtgcagca ctgaaaaata tcattgcaat tggtgccggc gctatccatg
     9601 gattaggatt cggagatgat gcaaaagcag caatcatgac tcgaggattg gcagaaatca
     9661 gccgtcttgg tgtcgcgatg ggtgccaatc ctctgacatt tatcggtttg agtggtgtgg
     9721 gggacttgat cgttacttgt acaagtgttc attctcgcaa ttggcgtgcc ggtaacttgt
     9781 taggacaggg acataagctg gaagaagttc tagaaaacat gggaatggtc gttgagggtg
     9841 tggcaacgac aaaagcagca gtagagctag ctcaacagct aggtgttgag atgccaatta
     9901 ctcaaacaat ctataatgtt ttgtataatg gagaagatat caaaaaagca gcaaaagaaa
     9961 tcatgttacg tgacggaaaa atggagaatg aattttctct tcgttaaata gatgatcaat
    10021 agttaatcgt
//
LOCUS       DAZKOB010000097         9810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000097 DAZKOB010000000
VERSION     DAZKOB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.099"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..2153)
                     /locus_tag="ACG8M6_002337"
     CDS             complement(33..2153)
                     /locus_tag="ACG8M6_002337"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0457958.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2383..3561
                     /locus_tag="ACG8M6_002338"
     CDS             2383..3561
                     /locus_tag="ACG8M6_002338"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0457959.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3577..4335
                     /gene="aroD"
                     /locus_tag="ACG8M6_002339"
     CDS             3577..4335
                     /gene="aroD"
                     /locus_tag="ACG8M6_002339"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0457960.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4358..4843
                     /gene="ybaK"
                     /locus_tag="ACG8M6_002340"
     CDS             4358..4843
                     /gene="ybaK"
                     /locus_tag="ACG8M6_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0457961.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4914..6167)
                     /locus_tag="ACG8M6_002341"
     CDS             complement(4914..6167)
                     /locus_tag="ACG8M6_002341"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0457962.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6284..7633
                     /gene="gdhA"
                     /locus_tag="ACG8M6_002342"
     CDS             6284..7633
                     /gene="gdhA"
                     /locus_tag="ACG8M6_002342"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0457963.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7745..9091
                     /locus_tag="ACG8M6_002343"
     CDS             7745..9091
                     /locus_tag="ACG8M6_002343"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0457964.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9399..9785)
                     /locus_tag="ACG8M6_002344"
     CDS             complement(9399..9785)
                     /locus_tag="ACG8M6_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0457965.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3127 a         1782 c         2009 g         2892 t
ORIGIN      
        1 attttgtcgc tgctcctttt aaatttattt tatcattcgg aagaacttgt ggtcgttccg
       61 tgaagttctt ggtacgtttg cgttttataa agatcaacag ttgattgtcc gttgtgttca
      121 ctaaagacac tagttgactt gtcaccaaga gatttttcag cttcttttaa tttctctaag
      181 ccattcttgt agtcataatc actggtatca attgggtcta atccactatt cgtatagaac
      241 cgaagaagat caccattatt gatttggtca gaaacagcta actgtttatc aactttttct
      301 ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt cagtaatagc ttcaccagtt
      361 tcgttactat agatcgtacc accgtagtaa gtatattttg gtgtaacaaa atcaccatca
      421 cggaaagaaa caagctgttc atgatctttc gagaatagat cttgtcctaa ctgaatataa
      481 ttttttgtat ctacacctag taagtgaagt aatgtaggca aggcatcaac ttgtccgccg
      541 taagtgtgat tgattccgcc ctgtgtttgt ccagggatat ggatcatata agggacacgc
      601 tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg tttttccaac taattcagct
      661 agatttttat ttcttgaatt cgagacaccg taatggtcac cgtaaaggac gatgactgaa
      721 ttatcataca agccgctttc tttcaaataa ttgaagaatt cttgtacggc ggtatctaaa
      781 taattggctg tcgcaaagta accgttgatc gtttcatcat ttgtattggc taacgggaat
      841 cctgcttcgt cattggtgaa ttttgaataa ggataatggt tagatacagc aatgaatttt
      901 gaataaaatg gttgttgcag acgttccaag tattgtacgg attgttggaa aaatggttta
      961 tcatgcaacc cgtattggaa cgaattgtca tcattaacat cataataaga agaatcaaag
     1021 aaataatcat atcctaaatt cttgtatgtc tcgtttcgat tccagaatgt accagcgttc
     1081 ccatggaata ccgcactaat gtaaccttgt gtttgactta aaatatcagg tgcagcttgg
     1141 aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt gattcaagcc aaacaatgaa
     1201 ttttccatca gcgtttccgc atcgcttgtt tttcctgctt ttacttgatg gaaaaagttg
     1261 tcaaaactga acgtgctgtt gctgtggtaa agactattga tgaaaggtgt aacttcatgc
     1321 tggagaccat tctcatctgt caatttataa tcgatcagga attgctggaa gctttctaaa
     1381 tggatataaa tcacattttt tcctctagcg agcccgaaca aatcgtcatt tggtgctgca
     1441 taatgttctt ttacataatt ttctacttct ttcatatcat tgggacttgc ttctgcccgc
     1501 acttggtttg tctggtaagt ttgaacaccg tcataaacca tgaaagcatt caagccaaga
     1561 tatttgacca aataatcacg agagaatgtt cgagtcagca attctggacg atcagcttcc
     1621 gccataaaaa gatttcctga gaaaatcata actgctaatg tagagatcgc aaaagccatt
     1681 cttgctcgga tcgggcgagg gtccaacttg attttttttg caatcagcag aacagtcaag
     1741 ataataaaat cgatccaata gatcacatca tatgggcgaa acagtcgaat ggcactttct
     1801 cccagaccac tcgctacttt cccagcacct aaaatcgtgt tgatcgtaat gaagtccgtg
     1861 aattctcgat aataagtgac attcgaaaaa acaagcaatg tcaaaagaaa ataaatgacc
     1921 atcatcgtga tataagcaac tctagggcgt ctgatataaa gagcaattcc aagtaataac
     1981 aatgtagttg caatagggtt gataaacaag ataaagtatt gaatgccact ttcgattccg
     2041 agatggaaat caactaaata ggcaaaaatc gttttcaacc aaagtaaaac gactagtaaa
     2101 gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt ttatacattt caagataatc
     2161 ttcctttctg cacttcttaa tcacagaagt gtcctgaaac catgttacac agctcactta
     2221 ttctcattta ttttactagt atgacaatat agtgtcaatt gaaaccagaa gtttatgctg
     2281 tttcattaag gttttcttta agcttcatga aaaaatgagc agtaagacaa gtcctatgtt
     2341 ttttgttata cttgggaaga gaaagctaaa gcaggtgaag gaatgaaagt aaaagtaaaa
     2401 aaaagagcaa gcaatcgatt gaaaaaagga tatccactga tccaaaaaga agaccttgct
     2461 catccccaaa aagaaaccac gcaatgggtg gaattagttg attcgcaaaa ccaatttatc
     2521 gcaaaaggat atctaggaaa gcagaataag ggaatcggtt gggtattgag ccagcagaac
     2581 atcccatttg atcaatcttt ttttgaagaa ttttttgtga agtcaaaaga aaaaagaact
     2641 ttcttttttt cagatgattc tactaccgct tttcgtatat tcaatggaga aggagacggg
     2701 atcggcggtc tgattgttga ttattatgca ggttatgctg ttttttcttg gtataacgaa
     2761 acgttatatg cacatcgtga attattgatt caagcttttc gtgaaatcta cccggaaatc
     2821 ataggaggct atgagaaaat tcgatttgaa tcagtaaatc tgcctgagag ccagttcatt
     2881 tacggaaaag aggcaccgga acccttgatc gtaaaagaaa acggagtaaa atttgccgtt
     2941 tatttaaatg aaggattaat gactgggatc tttttagacc aaaaagaagt acggggatca
     3001 ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca tgtttagtta taccggcgcc
     3061 ttttctgttg ctgccgcaat gggaggggca atcgaaacaa caagtgtcga tctagcaaaa
     3121 cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg gtttggatgt cgatcagcaa
     3181 aaaatcattg ttatggacgt attcgagtat ttcaaatacg ccaaaagaaa acaattttta
     3241 tatgacatga ttgtattgga tccaccaagt tttgccagaa ataagaaaaa agtctttcgc
     3301 gtagcaaaag attatgggga actggtcaag gatagtctgg atattttagc agaagacggg
     3361 atacttattg cgtctacaaa tgctgcgaat gtctcgatag aacaattcca aacaatgatt
     3421 gaagaggaat tccatgccaa aaatgtttct tttgaacaac ttgctttata tcgtctgcca
     3481 aaagatttcc agacgacgga taccttttta gaaggcaatt atctgaaagt gtttgtttat
     3541 caaattaaaa ataaccatta gaaggagcga tgcagaatgg ttcgggtaaa gggattgaat
     3601 ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg gggccgtccg agaggaagta
     3661 ttacgtgaag cagttcttgc acgtgaagca ggggccgatt tagtcgaatg gcgagtcgat
     3721 cattatcgtg aagttttttc ttttgatgaa gtagcagaaa cattggttct tatccatgag
     3781 atgcttgatg gactgccgtt attatttacg tttcgtacct tagctgaagg cggagaacaa
     3841 gacatatcga tccgtcagta tcgcgacctt tatgaacatg tgatacacac aggattagta
     3901 gatatggtcg atatcgagtt gttcaaagta gaaagtctag gaaaaagact gatagaggaa
     3961 atcaagtctt taaacattcc tttaattatc agtagtcatg attttaaaga aacaccagca
     4021 gaccctgtct tgctttaccg attgaatgtt atggaacatt ttggagccga tatcggaaaa
     4081 ctggcagtaa ctcccaataa tgaacgagat gttttgcggt tgatggagtt gactagacgg
     4141 gcaaatgctt ttttctccat gcctattatt acgatgtcca tgggaaatct tggtaaaatt
     4201 tctcgtctgg ttggcggaac gacgggttcc gttatgacat ttggttcctt aagcagcgat
     4261 tctacttctg ctccaggaca attaccggtc aaagcattga agcaaattat tcaattatta
     4321 aatgaaccgt tgtagaacaa gtttgaggtg atattttttg gcaaagaaaa aaatagtaaa
     4381 aacgaatgca atccgtatgg tcgaacaaaa aaagattccc tatacagagc acgaatatga
     4441 gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa cagcttccag aaagccaatc
     4501 tcgtatattt aaaacactgg ttgctgtggg caatgtgacg gggcctcttg ttgctgtgat
     4561 accgggagaa gccgaattga atctaaaaaa attagcaaaa gtttcaggga ataaaaaagt
     4621 agaaatgctc catttaaaag atttggaagc aacgaccgga tacatacgtg gtggctgctc
     4681 gcctataggt atgaaaaaac tatttcctac ttatttagat caaattgcag aaagctatga
     4741 acaaatcatt gtctcggctg gcagaagagg gctacagatg gagttagcac ctcaagatat
     4801 ctgtgcatta acgagcggac aatttgcaga tataaaacaa taaatcatac gtataaaaga
     4861 aagaaggagg ccgggatgtt gtttgtccag tctccttctt tcttttttat acattatgct
     4921 ctttgcccgg cattcattcc tgctacatgc cctgtagcga aagccgctgt gatattaaag
     4981 ccgccagtat aaccattgac atccagtaat tctcccgcaa aatacagacc atcaatgatt
     5041 ttactctcca atgttttagg ataaatttcc tttagagaaa cccctccgcc agttacaaat
     5101 gatttctcaa taggaaaagt tttgttgatt gatagcttaa actctttgca tagttgaaca
     5161 aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca gatgatccat ttcaagccgc
     5221 tcaagaaaga aaactaacaa tcgctcaggc aatataccat gccaagcatt gaccaagttc
     5281 tttttggttg ctttactttt ttctgtcaat atatgaataa gctcttgttt ggtttgtgta
     5341 gggaaacaat ccagagaaag tgtcactggt tctcccgttt tctctaattc tttattgaca
     5401 aaacttgaac aacgcagtgc agccggacca gaaataccga aatgggtgaa aagtaaatcc
     5461 atcgtatgtt cagtcaatac acgacctttt ttatttaaaa cgcgtaaagt gatgtcttgt
     5521 aaagaaagcc cttgaagtgt tttctcctga atatacggtt catccgatat caagggggac
     5581 tctgtcgcat aaagaggcgt caccgtgtga cccactcgct tcacgatctt gtacccatct
     5641 ccagtagaac cagtagatgg ataagtccgt ccaccagttg ttaagataat ggcaggagca
     5701 tggaactctt cgaattccgt gcgtataccg taaattttat gttctttgtg gatcaatttc
     5761 tctactcgag tagagaatag aagcgtcaca tctagttctt tcagacgatt gagcaatgct
     5821 tcgataatcg tttttgattt atttgttaca ggaaacatcc gtccatgatc ctcttctttt
     5881 aaatgtacgc cttgtgattc aaagaagttc atgatatcga aattattcca ttgcgcaaac
     5941 gtactatata aaaactttcc atttccagga atatgtgcaa tcaaatcatc aacaggacga
     6001 ttgttcgtaa cattgcaccg accaccacct gtcatcagta gtttttttcc tgcttttttg
     6061 tttttttcga tcaagagtac gcgtgcgcct tgttcagctg cactgatcgc tgccatcatc
     6121 ccgcttgttc cagcgccgac tacgatcaca tcatacagtt tttccattcc ctcacctcaa
     6181 ggaaagtgta gcatatctga agaaagaaaa cagaaaaaaa tgttttgagc caaaagagaa
     6241 aatgagctaa aatgagggaa gataagcaaa ggagcgtgga acgatgacaa acgcaacaga
     6301 atatgttcaa gcaattcaag aaaagctaca tcaaaaggat caagggcaag aagaattttt
     6361 acaagcaatc gatgaattca tgcccacggt tatgtctttt ttggacactc atcccgaata
     6421 tatcgaaaag aatatcttag aacttttaac ggaaccagaa agagttattc aatttcgagt
     6481 gccttggcaa gatgatgaag gaaactggcg agtaaataga gggtaccgta tccaatacaa
     6541 ctcagctatc ggcccttata aaggtggatt gcgcttccat cctagtgtca atctaagtat
     6601 cttgaaattt ttggcttttg aacagatttt caagaatagt ttaaccggtc tgccaatcgg
     6661 tggaggaaaa ggcggaagtg acttcgatcc taaaggaaaa tctgataatg agatcatgcg
     6721 tttttgtcaa agtttcatgc tggaattgtc aaaacatatc ggcccatcga ttgatgttcc
     6781 tgcgggagat atcggtgtag gagctagaga aatcggctat ttgttcggcg aatataaacg
     6841 attgaaaaaa tatgatactg gtgtgttgac aggtaaacct ttggattttt ggggaagtaa
     6901 gatacgtacg gaagctactg gttatggatt agtgtattat gtcaaacatc tattgaacga
     6961 agaaaaagat tcatttaatc aaaaaacagt ttttgtttcc ggaagcggga atgtagccat
     7021 ttatgcaatc gaaaaagtgc aagagctagg cgggaaagtt gttacatgtt cagattctag
     7081 cgggtacatc tatgatccag aaggaataga tgtgaaacta ttgaaagaag tcaaagaaat
     7141 caatagacaa cgtttgacgg aatacgcaaa ccaacgtcct tctgctattt atcatgctgg
     7201 agaatctgtt tggacattga aagaaaaagc ggatatcgct ttgccatgtg ctactcaaaa
     7261 tgaaatagac gaaagcttgg ccgaaatctt agtagaaaat gggatcaaaa tcgtagcaga
     7321 aggcgctaat atgccatgta cagtaaaagc tgtttctgtt cttcatgaag caggcgtgtg
     7381 gtattgtcca ggaaaagctg ctaatgctgg aggagtagca gtatctgctt tagaaatgag
     7441 tcagaacgtc cagcgattga cttgggaaag agagcaagtg gatgagcagc tggatcaaat
     7501 catggaaacg atttaccata cgtgtcgcca aacagcagcc gagtacggcg acgaaaaaaa
     7561 tctgcttctt ggagcaaatg ttgctggatt tgaaaaagtt gccaaagcaa tggcagcaga
     7621 aggtttagtt tgaaaaatga aactttcttg gaaatattgt cagcattttg tttatttgct
     7681 tgaaaaataa taaaaaaaaa cgtaaagtat tacctgtaga gtaaacctat aaggaggaga
     7741 acaaatggca cacattcatt ttgactattc aaaagtagca ccgtttgtca acgaacaaga
     7801 gttagggtac atgcaaagcc aagtaacagc agcccacaac gaattacggg aaggtactgg
     7861 cgcaggaagc gacttccgcg gctggatcga tttaccaacg aactatgaca aagaagaatt
     7921 tgcacgtatc aaagaagcag ctaaaaaaat ccaatctgat tctgaagtat tagttgttat
     7981 cggtatcggt ggttcttatt taggcgctcg tgcagcgatc gacttcttga atcacacatt
     8041 cttcaattta ttagatagca acacaagaaa agcaccacaa atcttctttg ccggaaattc
     8101 aatcagctca acatatattg ctgacttgat cgaagtaatc ggtgatcgcg acttttcagt
     8161 aaacgttatt tcaaaatctg gaacaactac tgaaccagcg attgcgttcc gtgtattcaa
     8221 agagcttctt gttaagaaat atggacaaga agaagcaaac aaacgtatct acgctacaac
     8281 agataaagca aaaggtgcag ttaaagtaga agcagatgca gaaggttggg aaactttcgt
     8341 gattcctgat gacatcggcg gacgtttctc tgtattaact gctgttggtt tgttaccaat
     8401 cgctgttagt ggtgcagata tcgatgcatt gatgcaaggt gccgctgatg caagcaaagc
     8461 atactcaagc gacaaactag aagaaaacga agcttaccaa tacgcagcaa tgcgcaacat
     8521 tctttaccgt aaaggcaaag taactgagtt gttgatcaac tatgaaccag gaatgcaata
     8581 cttctcagaa tggtggaaac aattgtatgg tgaatctgaa ggaaaagacc aaaaaggcat
     8641 ctatccttca agcgcaaact tctcaactga tttgcactct ttaggacaat tcatccaaga
     8701 aggacgtcgc aatatttttg aaacagttgt gaaggttgaa aaacctcgta aatcaatcac
     8761 aattcctgaa caagcagaag atttagacgg tctaggctac ttgcaaggaa aagaagtgga
     8821 cttcgtaaat acaaaagcat ttgaaggaac attgcttgct catacagacg gtgacgtgcc
     8881 taacttgcta gtcaaaattc cagaaatgga tgcttataca ttgggttata caatgtactt
     8941 ctttgaaatt gctgtaggta tttcaggtta cttgaatgga atcaacccat tcgaccaacc
     9001 aggtgtagaa gcttacaaga aaaacatgtt cgcacttcta ggcaaaccag gctttgaaga
     9061 tcttgctaaa gaattgaacg aacgcctgta agataagggt ttttcgctag atttaaataa
     9121 tcagtgatag aaacatgttc gctttaaaat gaaaactggt acccatttag gtactcaaac
     9181 atactgcatc gtttattcag aataaggggc tgtgacaaga cgtttgtcac agtcccttgt
     9241 ttgtatagaa atcgattgat tgccttgctc tttagtttgt tttaggactt taagaacagt
     9301 gaggctgact tcgaaggtta attctgtttt tttgttttct agaatcagta agcaaaaaag
     9361 cactcacttc gagtgctttt ttgcgatata ttcttagctt aagataggcc taatttttct
     9421 tttacagatg agggtacctt atcgctggta attacttttt tctctatgat ccgcttttcg
     9481 cttgctttca actgcaagat cgttccttcg gggtaagcag aaccattggc agagtcacca
     9541 gaagtaatga catcgaaatc taactgtctt ttttcaccgt tttcagcata agcatctacg
     9601 ttgtatttaa aacctcttcc aattacttcc ccattatctg ctttgatatc agtttctgca
     9661 ggcattggag ctttgactac cgcataataa tctttaccta cataagtaga ctgatagtag
     9721 tcccaacctt tccatcctat aaaagcaatc aatatgataa cgatcaaccc taaaattttt
     9781 ttcatgaaag atctcctttt ttatttttat
//
LOCUS       DAZKOB010000098         9765 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000098 DAZKOB010000000
VERSION     DAZKOB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.100"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..1231)
                     /locus_tag="ACG8M6_002345"
     CDS             complement(164..1231)
                     /locus_tag="ACG8M6_002345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0457966.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(1251..1730)
                     /gene="pssE"
                     /locus_tag="ACG8M6_002346"
     CDS             complement(1251..1730)
                     /gene="pssE"
                     /locus_tag="ACG8M6_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0457967.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(1747..2208)
                     /gene="pssD"
                     /locus_tag="ACG8M6_002347"
     CDS             complement(1747..2208)
                     /gene="pssD"
                     /locus_tag="ACG8M6_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0457968.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(2222..3472)
                     /locus_tag="ACG8M6_002348"
     CDS             complement(2222..3472)
                     /locus_tag="ACG8M6_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0457969.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(3850..5301)
                     /locus_tag="ACG8M6_002349"
     CDS             complement(3850..5301)
                     /locus_tag="ACG8M6_002349"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0457970.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(5622..6386)
                     /locus_tag="ACG8M6_002350"
     CDS             complement(5622..6386)
                     /locus_tag="ACG8M6_002350"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0457971.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(6414..7112)
                     /locus_tag="ACG8M6_002351"
     CDS             complement(6414..7112)
                     /locus_tag="ACG8M6_002351"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0457972.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(7124..7903)
                     /locus_tag="ACG8M6_002352"
     CDS             complement(7124..7903)
                     /locus_tag="ACG8M6_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0457973.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(7919..8863)
                     /locus_tag="ACG8M6_002353"
     CDS             complement(7919..8863)
                     /locus_tag="ACG8M6_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0457974.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(8909..9688)
                     /locus_tag="ACG8M6_002354"
     CDS             complement(8909..9688)
                     /locus_tag="ACG8M6_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457975.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2800 a         2043 c         1492 g         3430 t
ORIGIN      
        1 cgtgcttgta ctcttgctct attacaagca ctaaaacact actattcatt tgaattctta
       61 cacggataaa aatttacatc gtctatttca atattttttc tctctatttt attcacttaa
      121 taagttcatt acataatcga aacatttttt attttccttg tcatcactcc ttgctccctt
      181 tttgatacag atttaaaatt ctatcaaaga aatttccatc gttaaatttc gttattgcca
      241 aatccttcga acaacgtgaa aattttacgt atttatcttt atcattgatt tttagtattg
      301 cagatgtcaa cgctgatacg tcaccaggtt gaaacaaata tccattttct ccatcacgta
      361 ctgctgaaga aatatctcct acatctgtag caattacagg cattccataa gaaatcgcct
      421 ctagaataga tataggcaat ccctcgttat aggacggtaa aatcatcaca tttgatcttc
      481 tatagcactc ctcctttttt tctcccacaa cccaacctgt aaattcaaaa tacgattgca
      541 aattatattc ttctatcaat tctttgagcg atatttcttc atcacctgct ccagcaatct
      601 caaaaataaa ctttttttct ggatattgtt ctgcaatgtt tttaactgct tctaccaaat
      661 cgtgtacacc ttttcgcttt atcaaaactc ccataaacag aaatcggcac atttttgaat
      721 tccactttat ggtatattct ggaactacaa tagaattttc tacaacaaca gttttacaat
      781 ttggctcaat ttctttaatt atcttcttcc atttttctcc aagtacaatc gtggaatcag
      841 actctcttaa cagcaagcga atctgatttt gtttttttac agaaacacta tcataccatt
      901 ttttaaattc cgatccatga agatggatga ttacaggaat atttaataat ctaaaccact
      961 tattcagcac atatttccta ctaaaactcc ctctgtacga catgtgcata tgaataatat
     1021 taggtttact caataaagag atacctataa ttttagcaaa tgcatacata aaatataaag
     1081 atttaaatat tgcatttcca cccttataac tcggtataaa agtcatctcg atattctttt
     1141 tttcccaatt aaaattgagc atttgtgtaa ttatacttgt aattccacct tttaccgagc
     1201 tgtcattacc aatcataagt actctttgca taacgcttta ctccttatat ttatattttt
     1261 tctattaata cctctatcga attaataatc gtttccgtgt tagatacata cttgttaaac
     1321 atccgaactc ttgtttcttt aacggcatct tctaaatttt ctaattcata acaaggatag
     1381 ataatattca tctcatcaaa ttgtctaatt aactgcagtt ggtgatcatc gacatgctca
     1441 ccatacttag ccaatcgagg aacagcaatg acccttttac ctttttttac ggccccaatt
     1501 atagcacctg tacctccatg agtaatcacc atttctgctt tttgcatagt ttcagtaaac
     1561 tcatccctat ttagaaatgc tttataggaa taatttagtg gtttataatc actataacca
     1621 ctctgtgcaa atgtactttc gattattact cctttttcaa ttaaatcatc aatagctttt
     1681 aacaatctat caaattgaaa tttttgtgaa cctaatgtta taaatatcat ttttttctcc
     1741 tattatttaa tatatcccac ccaaataaat agcattagga taaaattgta gcatagtttc
     1801 ccactgaaca taaaattgat ctgcaaaatg atacatcaat tttcctgtct gagtagggct
     1861 ggttactttt gcaaacgatt caatataaat cagttttttt cccatcattt ttgttattaa
     1921 gcagataggg atcatagcta aaacacctgt acaaatcacg agatccggtt tttcctttat
     1981 atatataata attgatttga ataaattaat aagtatagta cacaaaaaaa acttttcttt
     2041 tctatttact tgtctcatgt agtacgtttt ttcgcctttt gactcagctg aatattctgt
     2101 ctgttctgtt ataacaaaac tctcatactt ctccatcagt ggttttaaca tcattaactg
     2161 ttcataatgc cccccagagg aagccgcaaa acatatcttt ttgttcatat taacctctct
     2221 atcagtcacg ctgaaaaata tctctagtgt aaactttttc tttaatatca tccaagcagg
     2281 tatcatatcg attggcaata atagaatcac acatcgcctt aaactgctct aaatcgttga
     2341 taaccttact tccgaaaaaa gtctctccat ctttcagtgc aggctcataa atcaccacag
     2401 tcgcaccttt agcttttacg cgcttcataa ctccctggat tgaactctga cgaaaattat
     2461 cactgttgct cttcatggtc agccggtata taccgattaa aactggattt tccctgttga
     2521 catcatactg attatattca gcgtagttgt aatagcccgc catctgaaga acacgatctg
     2581 cgatgaaatc cttacgggta cgattagatt caacgatagc ctcaatcaaa tttccaggaa
     2641 catctgcata gtttgccaac aactgcttgg tatcttttgg caagcaataa cctccatatc
     2701 caaaggatgg attgttgtag tgagttccaa tacgaggatc gagacaaaca ccattgatta
     2761 tctgctgagt attcagcccc ttcatttcag cataagtgtc taattcattg aaatagctga
     2821 ctcgcagtgc taaataggta tttgcaaaca gctttactgc ttcggcttca gtgaatccca
     2881 taaaaagagt atccacgttc tccttaattg cacttttctg cagaagtgtt gcaaaaacat
     2941 gagcagcatt taacaagcgc tcatcagtca tatcagtacc aacaataata cgagatggat
     3001 acagattatc atataaagcc ttgctctcgc gcagaaactc tggactaaaa ataatgttct
     3061 tactccccgt tgtctcacgg actgattcag tgtagccaac aggaattgta gacttgatga
     3121 ccatgatggt atcaggattc acgtccatca caacctcaat aactgactca acagcagaag
     3181 tatcgaaatg ctgagtacga ctgtcatagt tagtcggtgt agcaataaca acgaaatctg
     3241 cggatttata agcgctcctt gcatccaaag tagcagttaa gtcaagttct ttttctgtaa
     3301 gatacttttc aatatattca tcctgaattg gagacttccg tttattaatc agttcaatct
     3361 tttcagggat aatgtctacc gcggttacct ggtgatgctg cgccaatagt gtggcgatac
     3421 tcaaaccaac ataaccggtg ccggctactg cgatgttgta ctttttcttc acaatatctc
     3481 ctctttcctc tcttaagtat ggagaatgtt actcctacgg ctattcatca tgtcaaaata
     3541 acacagctta gaatgttgat aaaataattt tccagaatgt gataaaatct ctttcattta
     3601 taaaccacac atctaatttt atctattctc cacaagacat gtagagtatt ctttaataac
     3661 aagatacgac cttactaaat acttcaaaaa attatcatat ttaattcata aatataaatc
     3721 ttcattttaa atgtatgccc aaaaatttat ctgtaatgcg tcatcaaaat ctagattcca
     3781 aatctttatc aatttttatc cactcattct accactttag taatattttc tatttataac
     3841 actttcgtcc tacattgatc cccccctctg aagtacagtt tttactgtct taaataaaat
     3901 tttaaaatct aaccccatac tccaattttt tatatattcc gtgtcaagtt ttgctacttc
     3961 ttcaaaatca gtaatatcac ttctaccact tacctgccac attccagtaa ttcctggctt
     4021 aatagcaatt cttgcacgat gatgataatc atattcttca tactctgatt taaggcatgg
     4081 tcgaaatcct ataaggctca tctttcctgt tagaacgttg aaaaattgag ggaattcatc
     4141 taaagacgtg gatctaataa attgaccgat tcctgtcttt tttgttccat ctggaagtat
     4201 tttatttccg ataattcttg gatcaaactc catcttaaac ataagattat tgctcatctt
     4261 attttctttc attaattcta cttttctttc ttctgcatcc atatacatac ttctaaactt
     4321 atacatcttg aacggtttac cattctgacc tatacgttct tgtgcaaaga aaattgggcc
     4381 tggtgaatta atataaataa gtggagcaat aaagataaaa ataattcccg ttgcaataca
     4441 tccaacaata cctgcaagaa tatcaattgt tcgttttata aatgcctgtt gagttgtcat
     4501 aacattgata cttgttgtaa gtactgtata ttctcctata cgaccaatag attgttttcc
     4561 caaagcacta tctgatactt ttgcaagatt caaatgtaca gtaaggccca tttcagagca
     4621 tcgttcaata aattctttca tacacgaagt acttttattc aaaataataa agacttcatc
     4681 tacccatccc tgacataagt aatccgctgc atttttggaa tcagaaacaa ctgtcactcc
     4741 ctcaacatct tcaccaatca tgcttttatc tatgataatg acaccattta ttttatacat
     4801 tttatagtta tgctctttca cattacgaat aactacttca gcaatactac tagatgtaac
     4861 tataataagt gatttgttcc cctcattttt cattttatgt ttcaagtaat gtttccataa
     4921 gaggcgagtt atatatgtaa ttacaacgta taaacctccc atcaagaaaa gaacgactct
     4981 agaataatca tttccaccct gaacactaat gagatacaaa cttcctaaca gcaccaacat
     5041 aatcgcttgc tttacagaag aagcaaattc tatatagtac cctcttttta atacacctct
     5101 atatgcttca aacatgaaaa taactaaaag atctgccagt tcaatgaaga ctgccatgtt
     5161 ttgatacatt ggaatcacat aaggattcca atctacctgg cgtaagacat acgatagata
     5221 aaaagcaagc tggatacaca ataaatctaa aataataaaa tcaatatgtt taacccatcc
     5281 ccttgaactt ttcttgtaca ttccttttta ctcccctctt tttaataaaa taaattgtat
     5341 tacttgtttt ctctcctaaa tatctttctg aaggattatt gactcgacaa attggcttgt
     5401 ggatattctt atatatagaa catccgtaag ccaacttaaa actccttaac aaaatttttt
     5461 tactcatatt gtagatcaaa acaagtatcc aagaaatcat attttatttc ttttttctgc
     5521 ttactcaaca acagctaaaa gaaaaatcat cataaacaaa ttcaactaag ccaacgatcc
     5581 ccttgtgaaa tcaagttttt tcttgattta cgaataaaga gctaaaataa accaaacttc
     5641 ttctttttta cctcttcata gactggataa ctgatttcat ccccgttgat cagatccttt
     5701 gcgacttgtt tcatctgtac tactttttcc ttaccaaagt cctttgcaat ctgctcatag
     5761 cactctttta agtaaaaggt gcgcttagag accccatggg catcagaagc agccatctgc
     5821 acgagattat gtttgaccat cgttttggct gttttctgga tgtctttgcc aaatacccca
     5881 acataacttg gcgcagtgag ttgggccaaa catcccatat ctaggaacgg aagcaaatga
     5941 tttgggtctt cgcgaaaatg cacgttgcgt tctggatgga cgattactgg tgtctttcct
     6001 agttctaaca actcaaaaaa aagccgttct gcatataacg gcacttccag tgtcggaaat
     6061 tcgatcagaa gatatgtatc atctaaatct gtaaataata tttcatctcg gtggatgtct
     6121 tcgatgagcg ttccggtaat acgtacttct tgtccttcca ataacgtcaa aggcaactgt
     6181 cttttctcta attcggcttg taacgaagcg actagagaaa tgacttgtga tttttcattg
     6241 ctgtattttc cattattgtg gtgtggtgtg cataaaatat gcgtgatgcc ttgctgaatc
     6301 gctttttcag ccattgcaat acttgcttct aagttttctg ccccgtcatc gattcctggt
     6361 aggatatggc aatgtagatc gatcatttct cttgtctcct ctcttcttat tccttaattt
     6421 ccgtagtaat aaccagcatc tttgctatgt tctgctccgt tgtacacaac acctaagact
     6481 cgtgcttgta ccatctccag cagttctttt gctttagtca aggattcttt acgtgacata
     6541 ttttcacgta cgaccaataa agtgccatcc gtttttaaag acatgatctg tgcatctgtt
     6601 acggctacta ctggtggcat atcgaatatc accacgtcaa ataactgtct tgcttcggat
     6661 aggatctgat ccattcttgg cgagcttaac aattctgatg ggttgggtgg cttaggtcca
     6721 ctaggcaaaa tcgacaagtt ctcgactggc gttcgttgga tcacatctgc tacacttcct
     6781 gatgaactaa gtgctgtact taatccactg gcattgttca gctggaatgt cttatagacg
     6841 acgggtttac gtaaatctgc atcgactagt agaactcgtt gacctgattt agcaaaaacg
     6901 actgcaatgt ttgctgcagt ggtggatttc ccttctccag gacctgatga agtcacgaca
     6961 atcgttttga tttgctgacc agctgcggag gcaaactgga tattcgtccg gatcgtacga
     7021 tattgttcgg cgattggtga agatgggttt gttaatgtga tcaaactgac tgcatgagtt
     7081 tgtgctgctt tttgtttctg tgttcgtgcc atttttcttc ctcctagact cttgagcggc
     7141 ttctgcgtga tggggctgtt ttatcgtttg tagcttttgg tcgtgcagga gctgttcctt
     7201 ttttacggat accagcgttc aattctttcg ctgacatttc tggaactgtt cctaagattg
     7261 ggaatcctaa tgtatctgtc acatattttt catctttcac tgtccgatcg agtaattcca
     7321 ataagaatgc cagacctaca cctaccatca ttcctaacat taagccagtc attaaattca
     7381 atttattatt tggcgaaaca ggtgtcgtat tggcaactgc acctgaaatg atcgagattt
     7441 tatcaacatt catcacatct tttgcgttct cttggaaaac ttgtgaagtt gtgttagcga
     7501 tctgttgagc agccactgca ttcgtactta ttgcctgaat cgagaacatt tgtgaatttt
     7561 ctgcctgatt aactgagatg gcatctttga tttgttcagc agtcattttc acattgtctt
     7621 ctgtttctaa acgatctttg acttcattga gcactaaatc actgacgatc atatctttat
     7681 acgtattgat catttgcagg ttcgtattga catcattgac atttgcagtg tctgattgtg
     7741 gcagtgtcac gatcaattgt gcttgtgaac tgtattttgg tgtcaagatg aaaaatgtta
     7801 cgattcctga gatggccaag ccagcaaaca ttgagatgat gattattacc aagtgtttgc
     7861 gtaatgtttc aaaaatttct ttcaagctga ttgtctcttc cattttttct ccactttcct
     7921 atttattctc tagattcatt tgatctttta attctttttt cacacgatca agttcttgat
     7981 cactgacttt ttgataggag atattatctt gcatgaagcc ttctccttgc agctgatctt
     8041 gcttaatcgt accaaatgcc tcccgataat ctaaagcaat ttttttcatt tcatcaaagt
     8101 tcatgtctgt tttcatattc gactctagag cattcaaaac ggattgatag ttgctgacac
     8161 cgtttagact caatactttt ttcgctacgc cttctacgat ctttctttgg cgcccttgtc
     8221 taccgtaatc accatttggg tcttcataac gcatacgaga ataagataat gcctgatccc
     8281 cgttcaatgt aattttaccg attggaaagt catagccatc ttgtgagaaa gtgaggtcat
     8341 tattgacttc gataccacca actgcatcaa cgagttcctt tagtcctttc atattgatag
     8401 agacatagtg atcgattggg atgtctacat atttttgtac ggtatccatc gccatggcag
     8461 ctccgccgaa tgcgtaagcg tggttgatct tgtcttttgt gtcacgacct acgatgtcga
     8521 catacgtatc tcgaggaatg ctgacgatcg ttgtctgttt gtcttcagga ctgactgtgg
     8581 caaccatcat cgtatctgat cttccttgat cgacccgacc taaatctcca gtatcaatcc
     8641 ccatcaatag aacagagaaa gattcttgtt tgtccagatc aactggtttc gttcgttctc
     8701 cttgggttcg ttccactgat tcgtaagttt tctcaataga atgtgagaga tccagatata
     8761 attttgctcc tactcctaca attgctaata ctaatacgat aagaactgct aaaataatca
     8821 aaaatatttt tttccctctt gattttcttc ttcgtcttgc catagtttcc tccgtttgct
     8881 aaccttgctt gaatcaacgt tttaactgtc aatagcttat attgctatag cccatttgct
     8941 gagcaacagt ttgtgctttt gttgcgtatg cggctcttat tttttcattc cggatctgct
     9001 ctatttgact gagcgccgtc aaatattgat cgtaagtagc ggtagctgtt acttgaccgt
     9061 ctttcagcat cgtatcgaat agctgatcta gttgcgtgac ttgtgcaatt tgggtatcag
     9121 cgaatcctag atattgcata acgactgttt tccaagcact atccttaaaa aagctcatat
     9181 tttcacgtac atgggcgaca tctgcacttg ccaacttttc tttgatgatg acatcatcca
     9241 cagcttgctg ccagtttgat acgtttttag taaataattt attgacagcc gtctggatct
     9301 tcagttttga ttctgcatcc tctaactgcg ttaagactgc tttcttttct tcttggatcg
     9361 cagcagcttc tttaggtaat tctgattcct tgatgtcata atcatctgcc gaaagtttga
     9421 tagaagatag tttatgtgac aaatttgaaa ctgtttcttc tgttatttca ggatttagga
     9481 aaattttttc ttttgtactg tacagcttat ctacttcttt tgataaagag gtcacggtgt
     9541 ctttttgatt ggtgatggca gattctgcgt aattgatccg ttgatgatat tgccgctgat
     9601 tattgagatg gtatccaaac aagcaaatca caatagcgcc tgtgcctacc gccacagtag
     9661 ccaataactt gacgaacggc ttttccatag attcactcct aaatattgat gatagataag
     9721 taaagatgtg attcattcac atcagaatga gcgcttttta tttta
//
LOCUS       DAZKOB010000099         9742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000099 DAZKOB010000000
VERSION     DAZKOB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.101"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..243
                     /locus_tag="ACG8M6_002355"
     CDS             31..243
                     /locus_tag="ACG8M6_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457976.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(276..470)
                     /locus_tag="ACG8M6_002356"
     CDS             complement(276..470)
                     /locus_tag="ACG8M6_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457977.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(483..665)
                     /locus_tag="ACG8M6_002357"
     CDS             complement(483..665)
                     /locus_tag="ACG8M6_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457978.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(662..802)
                     /locus_tag="ACG8M6_002358"
     CDS             complement(662..802)
                     /locus_tag="ACG8M6_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457979.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(1292..1657)
                     /locus_tag="ACG8M6_002359"
     CDS             complement(1292..1657)
                     /locus_tag="ACG8M6_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457980.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(1697..1999)
                     /locus_tag="ACG8M6_002360"
     CDS             complement(1697..1999)
                     /locus_tag="ACG8M6_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0457981.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(1999..2298)
                     /locus_tag="ACG8M6_002361"
     CDS             complement(1999..2298)
                     /locus_tag="ACG8M6_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457982.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(2291..2455)
                     /locus_tag="ACG8M6_002362"
     CDS             complement(2291..2455)
                     /locus_tag="ACG8M6_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457983.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(2461..2730)
                     /locus_tag="ACG8M6_002363"
     CDS             complement(2461..2730)
                     /locus_tag="ACG8M6_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457984.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(2742..3491)
                     /locus_tag="ACG8M6_002364"
     CDS             complement(2742..3491)
                     /locus_tag="ACG8M6_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457985.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(3488..4180)
                     /locus_tag="ACG8M6_002365"
     CDS             complement(3488..4180)
                     /locus_tag="ACG8M6_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0457986.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(4186..4857)
                     /locus_tag="ACG8M6_002366"
     CDS             complement(4186..4857)
                     /locus_tag="ACG8M6_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0457987.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(4850..5191)
                     /locus_tag="ACG8M6_002367"
     CDS             complement(4850..5191)
                     /locus_tag="ACG8M6_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457988.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(5369..6067)
                     /locus_tag="ACG8M6_002368"
     CDS             complement(5369..6067)
                     /locus_tag="ACG8M6_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0457989.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            6154..6624
                     /locus_tag="ACG8M6_002369"
     CDS             6154..6624
                     /locus_tag="ACG8M6_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457990.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(6667..6846)
                     /locus_tag="ACG8M6_002370"
     CDS             complement(6667..6846)
                     /locus_tag="ACG8M6_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457991.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(6833..7171)
                     /locus_tag="ACG8M6_002371"
     CDS             complement(6833..7171)
                     /locus_tag="ACG8M6_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0457992.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(7187..7390)
                     /locus_tag="ACG8M6_002372"
     CDS             complement(7187..7390)
                     /locus_tag="ACG8M6_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0457993.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(7405..8181)
                     /locus_tag="ACG8M6_002373"
     CDS             complement(7405..8181)
                     /locus_tag="ACG8M6_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0457994.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            8480..8800
                     /locus_tag="ACG8M6_002374"
     CDS             8480..8800
                     /locus_tag="ACG8M6_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0457995.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            8818..9249
                     /locus_tag="ACG8M6_002375"
     CDS             8818..9249
                     /locus_tag="ACG8M6_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0457996.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            9379..>9742
                     /locus_tag="ACG8M6_002376"
     CDS             9379..>9742
                     /locus_tag="ACG8M6_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0457997.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQIS"
BASE COUNT         2813 a         1905 c         1505 g         3519 t
ORIGIN      
        1 tcgaaattaa atttaaaaag gtgatttatc atgaatatca acaatttagt aaaactcgtt
       61 gaacaacgtt ttaaagaatc aggattagaa caattaaaaa aagataattc tccagaatta
      121 ttttctaatt ttaccgaaga cgacattcct actcttaaac aattaaaata ttattctgat
      181 tattggacgc aaactcgtga gaatgaatta aatttagaaa aactcattat taaaaagaaa
      241 tagcaactcc agcccacacg gctggttttt tagtttcaac ttccctcctt acgggtcaaa
      301 atcatcggtt ccggcataat ctgatcatcc tttagcaaca aatcaggata ttttttcctt
      361 agcttcttat tgttcgtgta agttttcaat agccattggt tggcttcgct tttgctttct
      421 ccagaaaaat aaattttccc tgtttggatg cctgcaagtt gaatcatcat tttcacctca
      481 aatcaaattt tttctgctag atggagcaaa agcaatccga catcaagaat cttttgaccc
      541 attatttcag ctactttgct cggcttaact ccttgagaaa agagcaagcg tgcttgctct
      601 atctcttcgt gagtccaaat aaatttggct tcctcaagga taattactgg atcagttgct
      661 ctcattgttc ctctggaaaa atgatgtcat tcttatgctt ggaccaggta tctgcatatg
      721 gaacaaagta ttgtctagct aattcgattt gatcaattaa agcagtttct gataattcat
      781 ggtctgcagc aatttcattc atactatcgc cttcatctaa acagataaga ccgttgacgt
      841 tatcgatttt tacatttgtg actgcttgac tttctccacg ctcataatat tctacagttg
      901 atactgatac atcagcttcg gagtttaatt gcagaatctg ttactgccgg gataatttaa
      961 tattcttttc ataattcacc ttttttccta ctgacatcga atatttttca tgttatgatt
     1021 agccgtgaaa ggagagctat attatgtgtt tacacatgat gtccttagct cattcggttc
     1081 tttggaagct tttttggaaa taactctcag gttcttgacg caaatctcat ttattctttg
     1141 aaacaaaaaa cttccatttt ccgagagctt tggatcactt caaaagagat ttgcatttat
     1201 atttctgtga ccttgagtcg cgtaaagaac cttacagcaa tggcggcaat tccactttca
     1261 ggaaaccgcc ttttttcatt gatattggtg gttagcggac tacccatttt ctttcggcag
     1321 tgccaaacca caaatgatct gtgttgatgt ttaggtagtt gtataccaga taagctaaat
     1381 ccaaagtgcc tgcatcttca agacgacaat attccaaaaa ttctcccata gctggacata
     1441 aaaatcctgg agctatcata tcccaacagt taatagtgca aagaaattcg tttagcttct
     1501 gaacgttttc tttggtttca cgaattacaa catactccaa ttcttctccg ctggcagagc
     1561 attccaaaac ttcaaattga ttaagaaatg gtagaacata tgcttcaaac atcaacattc
     1621 catctttctg tttttgcact tgttttgcat caatcatgtc tatcctccta ttcagttatt
     1681 tcttccatta atggaattat ttcaggtcac tggacttaac gaaaacacca ttgaccatct
     1741 ttccagtccg acctttgatt tcgtcataag caaaatccaa gcactcgtat aaatccatat
     1801 cattttgcat tgccaagatg atcaaggtta ctactacatc gccaattccg tctcttaaac
     1861 catgttcatc ttttctagct agagaagcgg caacttcccc gatttcttcg accgttttta
     1921 acatttgctt gctgaaatcc gcttgatcca atcccttatc ttttgcccac tgctctactt
     1981 tcgcgattag ttcgtccatt atttctcctc cacataccta aactgtcttc cttttgaatc
     2041 aatccataag ctcctagctc tatcccaaat aatatttttg ctcagaccag taatttcaga
     2101 taactgttta gcggtacccg ttactagaat tcgatcacca tgccagattg caatctttct
     2161 cggcgttctc cgtttgggct tttcagtcca cattgattta ccgagctttt ggacttctgc
     2221 aactattttt ttatcttcct gccaatcttc tgaataagtc aattcgataa ttcgctgcat
     2281 tgccgctttc ttatccatcc cgacattctc ctttcagtaa tttgagtact tgatcaagtg
     2341 cgctctcacg tccgccatga aatgtgttga gccacttgtc ttcgtacgac acactttgtc
     2401 ttaaagcttc ttgatgcatt aattcgatct gtgctgtaaa tgtctttagg tccatcttgt
     2461 ttacacctgc tcaagttcat taagatgttt ttgcaagcct ttaacacaat caatgaatag
     2521 taatttggta tatgctaaat ttcttaattg cgttccatcg atatagagtg cgaaatagta
     2581 tctgagttta ctccaactcg aacgatcatt cttaattcct tcaattccag cttcttcgag
     2641 ttgatcatat acgtctctca ggacgtctaa ttcctcgccc tttttataat tcgctatttc
     2701 attaattagt tctagataat cgatcttcat ttttccacct cttagaatgg tgcttttgat
     2761 tgtctattag ctcgttctag cgcttttttc ttgagatagg cttcttggtc gattgcccat
     2821 tcaggaagct tctctcgtct tcctgtgcgc ttgtatccac tgcttgcgtt cttaggttca
     2881 cttttttctt tccttgccca acttcgaata gttgccaaat agtttttata agtcttacca
     2941 gatgattcac aatactctga cagtcgttcg attcgctctt ggtaatcatt agggaattct
     3001 gttttgagtt tctccatctg ctcatctgac aaaagaacat ttttatactc tccgtattta
     3061 tgacggacgg gcttagcctt cgattttttc gaaggcggta actctcttat atattctttt
     3121 gtattattaa atgtattatt aatagatgta ttattatctt tgactttttc gtcaataggg
     3181 gtattgcgtt tttcgtcaat agggtattga ttaattcgta ggtacctatt gattatttga
     3241 ttggtaccct ctttgtaaat gatttcccga ttcaagtatc caaacttaat caaatcactt
     3301 acccatcgcg atatggtctc tttattcaca ccatataaat ctgcaaagta ctcattgcct
     3361 gcccaacaaa agcctctttc attacacaag gccgttatct ctccgtataa caacttagta
     3421 tttggtttaa gtcttttgtc gtaccttacg ttggctggta taatcgcata ataacttcga
     3481 tgttctgtca tttttcccct ccaatattta actttttgat tgttttctgg tttaatttga
     3541 tccctttgat ttgatattta tttttgaaat taatcacacc tattttgtgc ttctctgtgt
     3601 gatggattct gcagagtgct gcaaatgtgt actctgcatg atcaacttct ttgcgctttc
     3661 gtcttcctag cgctttgtca aagtgatcga tgtcagctcc tgttttgcca cagatgcagc
     3721 agactctttt tgtgatgcat ttgtagaagt aatattcttg attcgcaggt aaaatctcat
     3781 agccttcttt gaaaggaata tgatgttcaa agatgaaatc taagatgata tttgctaaga
     3841 cattggcatc actcacagtt gtattcgatt cgtctttcag gcttattttg cgccctgtga
     3901 caccttcaaa acgaaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga
     3961 taaaaatatc gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag
     4021 gatcaataaa acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta
     4081 gtcgatcaat gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt
     4141 tcagaaccgc tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa
     4201 ttcttttctc ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg
     4261 ttcaatagtt tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata
     4321 ttcgattaag gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt
     4381 ctctagcatg tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg
     4441 aaatacatat aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga
     4501 gttattgatt tgcattgcat ttggtttaac aactggttgg tttcgataat ctaaaacagg
     4561 aaataattcg gtttccgtgt gtcctttaac cgttactgag acagatacat aagtcccagt
     4621 ttcatccata agaaaaggtt tatattcctc aacaagaaag tcttgatgag ttccagaaac
     4681 aaccctgtag tgtttgtact cattaatagt taccgttgcc tgtggatcat tctttttcat
     4741 aatctcccac gcgtgagccc aagataaata atcaaaattt ccttttttct tgagaatttt
     4801 atttaactta cgactaaaaa gtttttcaaa gttcgttgtc cctttgattt cactcatcaa
     4861 attctgcctc catttcagca atgtatttct tacctggtcc gtaataagag atatcaatca
     4921 agttatctct gtcgtactct tctagcgcat caatcaagcc atcttcgatg acgtaaatat
     4981 attcaggttt attcgaatgc ttcgatagat ggataagata aacatgatcc caaatagtta
     5041 catggttgcc taaatcatct tgatcccaag ctagttcttc attcgtcaaa agatttcgtc
     5101 tgatttttcg accacttgtt tcctcaattt tcggcttgcc ccaatcagga tcaatcaaat
     5161 attgatctag agtggaaagt tctttttcca tatgctaaaa tctccttatg atgtgttttc
     5221 tttgtgactc tttgcttgcc ggcggagtca cttttttatt tgttgccatg ctttttgctt
     5281 gtcaatatgt tgttggctta ggatgattgg tttatagtat ttccaccagc aattagcaat
     5341 tgccgtccct attcttagcg cttcagctct attcattgtc atcaccaaaa agtctctgtt
     5401 gtctgttcag ttgatcaatt tccatacgga tcgcagtttc tggcaaccac atttcaataa
     5461 atgaaacagc atcatcgaat ctcttacgag gtaactcgcc atatcttggg attgaaaagg
     5521 tacgtttaaa ttcagaccaa aattttgaga atactttttt gctgatttct tcataagctc
     5581 ggctttcttt tccccctaga actcccataa ctttcatatt tcctttttgc ttaatttcaa
     5641 actcttgttg tccgctaatt cgcatagtat ctttaagcat ggaaacatct tttttaacat
     5701 ctttcatttc ttctaattga tagatcatca tatcttcaat tgtttgagga acagtattct
     5761 tgcgaataac atcttccatt tcgttgaatg cttcaatgta tttttgtttg aagtagatag
     5821 ctttctttcc tgtaaacccc atagccagca agaaaaatcc atctctacta atgaagaaaa
     5881 ctcgtcgatt tctgccatat gaatctggtt cattaccttc tacaaacatc tgtccaaaat
     5941 tggacacatc tttttttagt gcatcaatat ctcttaaaac atgttgatgt tttttctcga
     6001 agctttctgc cacttgtaag ctcgtagtca cagcttcttt atttttcaaa attactaatt
     6061 cttgcattat ttcttctctc ctttttggta taatttagtt aaaaactggt ggtgtttatt
     6121 ttgaattttt ttcacttaac aatttttcaa tggatgtcaa taataagttt tatattgagt
     6181 acgtttttaa cagggataaa aataaaagga atgcgccctc aactagaagt tgaacttaat
     6241 gcttcttact tcgctgcaga tatgatttat acaaaagtaa tcatttctaa ctattcaaca
     6301 gaaccagcta tgttagttaa tttagaactt tcatccgcta acttaaaccg cagatggtca
     6361 gctactccat ttaagaaatt aattgctaaa ggaggatcag tcgatgataa tagaatatac
     6421 tctgaagctg taccgctaaa tattcctcct aaaagtgcta tatcttgtta ccttgcattt
     6481 gaagtaggga aaattaattt taataaactc cttaatcacc aaacaaaaat gatatttact
     6541 ctaaatagaa cccaaatcca caaaattgtt gatatcaaaa atacaaactt cccaatagag
     6601 aaattagtga aagaattaaa ctgagtaata gtaatataca ttctagtttc tcttttctat
     6661 tcatttttag tcagccccct cggttggctt tttcgctctg tactcagctt cagcttcatc
     6721 aagccccata aaaatccaaa ccatgtaaac aatcgttcct atcaacgctt gcctgcttcc
     6781 ccaaagtcct aaagcgtaga tgattagcgg tgcgctgaac actagcgctc tattaaattt
     6841 tcccatccgc ttgcctcctt aagatttccg aaaaatttgt ttctaaaaat tcaagtgttt
     6901 tacttcttaa aaatagatat gtgtctcttc cttcaactgg ataatagaca aatccatttt
     6961 tgtttttctc gatatcgata atatttctat atcttgggtt ctttaaaact ctagaagtaa
     7021 accaatcata ttttctgtta atccgttcta gcacttctgg caacgtcatc catctaccag
     7081 tatcatcagc tttttttaac tcctcataat ccacttggga gataattaca tagccttctg
     7141 gaattgggat ttttgcttct agatattgca ttagctgttc ctccctttac aattcgtaca
     7201 tagtgataat cgaatctata attctgtttg cttctgcaga agtcttttta ccgtttaaaa
     7261 ttaaagataa gtagcttttg ctaattccaa atctttcagc aagcatggtg taagttaaga
     7321 actttgaact ttcgacatat tctttgattt tttctctatc tcgttgagtg atttctgcaa
     7381 tgtcagtcat actaaaactc ctttctaacc aatttcctct aaatccattt gagggtaata
     7441 tccttctttt tttagtaatt cgtagataaa tagacgccct ttctgtgtcc atttggtatt
     7501 cattacaatt ttagtgccac catcggcttt cgggatctca gttgtatgag attttgtgta
     7561 tccttggttc atgtgttttt tgcacaataa ccattggtta ccgacttttt tctgaatacc
     7621 tagtttatga agtaatttat tcatctgttg tggagacatc ccataatctg ctgcaatctg
     7681 actaattgtt actgaatctg tagaagataa tatgctatct aaataggaga ttttaggttc
     7741 gtactctgca atcttttgtt ctgcgattaa tcttccagtt ctttcttctt ttagttgagt
     7801 tgctaattga atgattgtat ctggattaag caaagcttct tctacttttt ctggagttag
     7861 ataacctcca tgttttctaa ttgctggcaa cacttcactt gttacccaac gtttaaattt
     7921 tttggcagag ggaagttttg attttaagat taaactgtac aaaccagatt cgttgatgat
     7981 tgtcatctct cttgattgac ctgaggtcgt gattcgcgac cccatcttat cttccaaatc
     8041 tacatgccgc gacaaagcat ctttagtgtt tgaataaccc aaaacactcg caacgtcttt
     8101 gcctacaaaa tatggttcat catttactaa aattgtccga acttcgtttt gttcgaaatt
     8161 aaaaatttgc ggtgtgttca tatttctcat tcctttcttt tatattcgta aacaaattta
     8221 acaacttttt tctaaattca attgacagta tctgagtaat actctatact aagagcataa
     8281 ttaaataaga cataaaaaca ttgattttaa agctttcttg gcggtctgca tttacatatc
     8341 aatagtgttt tttgttgtct ttttagttgt taaatctgtt tacaagatat agtatagctc
     8401 attactcaga attagtcaaa ttatttctgc gtatttttct aaactttttt tgtaaacatt
     8461 tagaaaggtt gttttatcga tgaataccta tgaaataata aaagacttgg cgaagaggaa
     8521 aaagatatct atacggcagt tagaaattaa ttttggatat tctaacggtt atctagcaaa
     8581 gtggaaaaat aatactccta attcagatga attaagtcgg attgctgact atttcgatgt
     8641 ctctgtagac taccttctag gtagagaaga aagagaaacc cctaaacatg tggatttatc
     8701 agaagacgat actgtttttt cttttgatgg aaaagaaata tctaaggaga caatgcgtaa
     8761 agcgattgca attgctaaag ctttagagga aaatgaatag ttggagtgat ggtttgtatg
     8821 tatttgaaat tgaaagaaat gttgagcgaa tacaatttga aagtagttta tatggagatg
     8881 aaagagcctg gtttctacta tccaaaaccc agaatcattt ttttgaatga aaatctatac
     8941 ggtgaaactg cagaagcctt tcatttatct catgaacttg cacatttcag cgcctctcat
     9001 tttgaatttt cagttttgta cgatacttct acaacttttc actcaaaatt tgaaacagaa
     9061 gcagataaaa tagctattct aattttatta aatatctata ttgagaacga actgactgat
     9121 gaatctcagt ttaatctcga aaaatttatg gagtattatt ctattcagaa taagctcaga
     9181 tacacttgtt atgctgtctg ccaatgctat tttaagaaaa aatattctta cgcaaggcaa
     9241 tatgtatgaa tacatcaaga attattaaaa aaaccgattt cagcaaaaca ttaaaaaagc
     9301 ccgtgctgca acacgggctc tttcctcata tatgagcttc tacaaaaaaa atcatatcac
     9361 agaaatgagg aagaaaaaat ggaaaaagag attttaataa aatctccagg aaaaaccttg
     9421 attaaagtaa ctgatgattc tatttcaatt attagaaaag gatttataaa tttagttaat
     9481 caaggtatta aaggagaaaa aactatcccc tttaaaaata tatcagccgt acaattaaaa
     9541 aaaccaggta tgagtaatgg ttatatacaa tttaccttgc taggcggaaa cgaaagtcgc
     9601 ggaggtatat tagcggccac gaaagatgaa aacaccgtta tgtttacaaa aaaatattgg
     9661 aatgaaatgg aaaatttaaa aaaatatata gagaagcaac aatctatatt agataataat
     9721 tccaaagaaa cacaaatttc aa
//
LOCUS       DAZKOB010000100         9737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000100 DAZKOB010000000
VERSION     DAZKOB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.102"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1635
                     /locus_tag="ACG8M6_002377"
     CDS             832..1635
                     /locus_tag="ACG8M6_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0457998.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            2220..2675
                     /locus_tag="ACG8M6_002378"
     CDS             2220..2675
                     /locus_tag="ACG8M6_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0457999.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2794..3852
                     /locus_tag="ACG8M6_002379"
     CDS             2794..3852
                     /locus_tag="ACG8M6_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFQ0458000.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3894..5843
                     /locus_tag="ACG8M6_002380"
     CDS             3894..5843
                     /locus_tag="ACG8M6_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0458001.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5846..9487
                     /locus_tag="ACG8M6_002381"
     CDS             5846..9487
                     /locus_tag="ACG8M6_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFQ0458002.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         3509 a         1397 c         1971 g         2860 t
ORIGIN      
        1 aaaataaaaa tccttttcaa taaaatacga aattataaaa attaccaaaa ttaaaaaaca
       61 aatatttttc tagtttttag aaataaattc acatactttt tctttttttg gatatattcg
      121 gaataccaca tcgctttttg tccacgtttg catcgctttt tgtccacatt cacatcgctt
      181 tttgtccatg ttcgcatcgc tttatgtcca ccctaaaatc tatttaagat tgatataaag
      241 cctttttgga ggttgctaaa gttttaaaga ttaaaatata tattataaat ataaataaaa
      301 ggggaagctg aagcttcctc cttttccttt ttttcaatta ttcaaagtta cacaagcaaa
      361 ataaactcct agatagtgtt agtttttttt aagatttttt atttttaaga ttttgaaata
      421 agagaggagt aatcctcctt tattaaatat ctgttaatat atctgttaaa tatctgttaa
      481 tatatctgtt aggggaggct taatctcttg ggagagtaag tattaaaggg gtataaaggt
      541 aacacttttg gacagtaaag gtaacaaaag tggacagtaa gggtaacagt tttggacagc
      601 aaaggtaaca aaagtggaca tttgaggtat cacactagga taagacgtta ccttcggttt
      661 tcttgatttg gaaggcgaaa aaatagaaaa ttagattagt aacaaatctt tgttgactgt
      721 tacctttagt tgtgatttaa tttaggtaac aagcagaaaa aattgtttcc tttaattagt
      781 accagattaa catgattaaa ggagcgcaaa aagaagattg gagagtaatc aatgtctcaa
      841 gaagttaccg aaaagattat tgatagtgat gtagatttag tatcagaaca aacaaatgca
      901 attgtgcgtt ataacaatgg tctgaataca acagctttgc gaggatttaa gcctgttgaa
      961 atggatgtgt tttgggctat tttatcaaaa atgaaacgtc aaggaacagc agaagtaact
     1021 tttgatttca acctctttcg ttatttggtc aaatacgaca gaagagaaaa ggacagtttt
     1081 tattctgtgt taaaagattt gagtgacaaa ctaggtaatt taacctaccg ttttgaagat
     1141 tcagacgtct atgaacagct gtggcttttt caaaaattcc ggatagataa acagaaggaa
     1201 acagttacaa ttaaggcaaa tgaggaattt gaatttatct taaactcaat tggcaaaaac
     1261 tttactcgtt ttgaacttga taattttaca aaactaagaa gtagctacac aaaagaattg
     1321 tatcgtcatc tcatgcaata cagaaataaa gagtggcgaa atggttattg ggtagtcaaa
     1381 gttgaagatt ttagaagagc attaagtata ccttcaagtt atagaatgac aaatattgat
     1441 aaaagaatac tagagcaggc gaaagaagaa tttttagctc ctgatgaaaa cggaatacag
     1501 gtatttgaaa cttttgatta tgaaaaaatc tatgcaaaga aaggaagaag agttgatcgg
     1561 ctcagattca cttttagtga gccagaatca aacttgccaa ccatttcaat gcataattgg
     1621 ttagaagaag attgagaaga agcaggttgt aattttaata caacctgctt ctttaaaaaa
     1681 gtatgggtaa cacttttgga caataaatgc aagaagtttt tttgtaagca agtttcagtc
     1741 taaataatat ttgaaagaat ggctaatcta tacaaatttt aacaactatg ggtaacactt
     1801 ttggacaagt caagaaaaaa aacaattttt cttaatttgt tgatcgacta aaggtaacac
     1861 ttttggacaa cacagaataa aaagtccggt gatatcagtt ttggtaggaa acatcttaat
     1921 atctttgtga gattgaaggt aacatttttg gacaagtcaa ctatttgtta aaagttaata
     1981 tagagtaatt agataagttt tttcttttcg tctgataaat ggataatatt agttatttta
     2041 ttgctaatta gtctttcatg atgactgaaa tagctaaaat aaatatctcg ttggataatt
     2101 gctgatactt taagggatat ctaaatttgc tgattgattt atttatgtta ttatgatatt
     2161 ataataacat aaatgtgtta cgcccgaacc tcattttaaa aaaatgaagg ggtgagacaa
     2221 tgaatgctat taattggcat gaatttgtta actatatgga gagcttacta gatgatctaa
     2281 aaagttttag tattgctgat gcagtaaagc atgggttacc agttagtaca acgatgaaat
     2341 tacgaggatt gaaaattcca gatgaaatta ttaagctgag aactaaaaca attattgaaa
     2401 ccgcaaaagt cgttgacaat atgaaccatg aactaaaagg caagaaaaca acttacgaga
     2461 aagattttga acgtttttta aaatctgtta ttggaaaata tctaggtaaa aaatctttca
     2521 gtgaattgag agatgaagag attgtaccta ctccaatggt tagcaaaatc cgaaaaaaca
     2581 aagccgaggg gaattactat cttggtacaa tagcaaaaat cattgattat ttggaaaata
     2641 aagagcaaaa gaaaggaacg aagaaaggaa gataattttt gtgttaaata ttaatgggga
     2701 agaatatacc gaaatgattc aaaatcccga acctagaatt accgaaaaaa cgtctgattg
     2761 tttgctcttt tatgaaaggg ggcagacaaa ctaatggaaa aaaacgtcaa ttcaacatct
     2821 accaaagatc aatacagtat tattggaggg ttgcttggtg ataaggataa gagaacacgt
     2881 cacggattac gcattatgct tgcaactttt ctaggagtgc tttttttatt aacaggcttg
     2941 ggtagctaca tttatcaatt aggaaactat aattattatc tcgtgaacaa tacaacacct
     3001 ataggctctt cattggagtt tgctcgatca ggagcagaga tcactatttc agatgtgtgg
     3061 acagatcaaa acagagatgt aacagtagta aaactcggtt actcgcaaaa cgctagaaag
     3121 ttgttatcta cgcaaggaaa aaactacaac ttatatatgg caacgaaaga aaaagatcgt
     3181 cctaaaaagc ttgaaatgag ctatgggatg ttaagtactg atggggatgc tttcttattc
     3241 ctaaaaggta aattagatga aagagcttat caagttttta ttgcaaacca agtatcacta
     3301 atttctggtg atgatacagc tgacgatgat accaaagtca atgtgaatga agcagattca
     3361 gttacaaaag cactttctca atattctatg aatgatgtta ataatgaagg agtaatttct
     3421 tttgggaaaa aagcaaaacc tgttaatact cctgataata tcaatttcag aataaatcca
     3481 tattcagatt caactaatgt ctatcaagga agctttctta ccaaagatgg cgatattgat
     3541 tattcaaaag ttgtagcagt tacaagtaag aataatgttg taagtaagtt agaagatcag
     3601 ttaaatgctt caaatactaa catcaagaaa attgaagcta caaaagctga gtatctggaa
     3661 cgtttacaac aaaatcctga cgataaagat tcgcaagaaa gtattcgtca actagacagt
     3721 cgaatggaga ccgaaattaa agcaatcaat gatacacaaa aattgattga tcaatacaaa
     3781 aatgctgact ttaacaaaga ttcttttgga gacatgaaga cgacatttaa gtacctaact
     3841 ctgactgact agaagtaaat tattcttatt ttagaggaag tgaacacaaa cagatggaaa
     3901 atagtaccga gttttttaag aatattcaaa ctgaatttaa tcatgctctc acaccaatga
     3961 aaccaccgaa acataagtta gtgggacgtg atgacgaact ccatgagttg tcactgatct
     4021 tgaatagacc ggaaacaccg caagctatct tgttaggtga agccggaaca ggtaaaacag
     4081 cacttgcaag tgaatgggct gaaagacagc gacaagatgg aaagaaagta caagtataca
     4141 aattatcaat cggacaattg cgtgttgggg gagatgatgt cctccaaaca cgtttagaaa
     4201 cattaattcc aagattgaaa gaatatgagc gaattttaaa atctgaggac aaagaaaatg
     4261 aatttgtcct cttcattgac gagtttcata aagttgttag ctcttttggt agtggcaata
     4321 aaattggggg tgattcgcta aaagaaccgt tagcaaatga atcacttcaa ttgattaccg
     4381 ctactacaag aaaagaatat gaaaactata tcaaaagaga tgatgcctta gggagacggt
     4441 tacaaccgtt agaattgaat gaactaaaaa gcgatgttgt cgtcgatatt ttgagacaat
     4501 ggctaaatac atcctctgtt aaagatgatc ctcaggcttc ttttaaatta tcgcaaagga
     4561 ttcctgatga attacttcaa gaaatcgtac aagcaaacaa gctttatcgt gaggatttgt
     4621 ccgaaccggc taaatcaatt gccgtaatgg aggatttgat tgcaaaagat cgagtagacg
     4681 gaaatgctta taatcgagaa agtctattgc aaatatttgg taagtataat attcagctag
     4741 attctgattt tgatatggaa aaacttcaag aggaattaaa gtccataaaa gggcaaccgt
     4801 tagcagttta tactataaca atgttgttta aacgtatgaa gcttggattt gatcgtttta
     4861 aaggtcgaaa gcaaccaaaa gcaactgctc tgtttacagg atcaacaggg gtcggtaaaa
     4921 ctgcaatggc taaagccgtg gcaagaggac tttatggcga tgaaaataaa gttctcttag
     4981 ttaatatgcc ggactacaaa acaaaagatg atgcaatgcg ttttagacga ctcataggta
     5041 ctcatgtaag gcatgatcct tctgcgatta ttgtgtgtga tgaatatgag aaagctggtg
     5101 tagaaacact cgatgccttt ttatcaattt tagatgaagg taggataagt tttactgaaa
     5161 gaggatggga tgatcgagaa gttcacgaca ctgtttcatt gtcaaataca attttatttg
     5221 cgacaacaaa tgcaggtgcg gagatgttcg attcgttaaa cagatatagc tcactatctc
     5281 gtgttacaaa caaggaaaat tctaatgaat atagcactga actaaaaaga gaataccgaa
     5341 aagtcgcatc agggatcaaa caagccttga ttggtgaagg tctaaagcct gaagttatta
     5401 accgatttgg acgaatagtt ccattttggg ctttatccga acaaactctt ctggaaattg
     5461 cttataatga aatgttgaag agtgctgaat tagtacgaaa acatttaaat atagatttac
     5521 gtcttccacc taggaaagaa agagtatatg gtcaaaattt ttatgcagac gatctctcca
     5581 tgcacttagt ttttgacatg gcttcacaag aggattctaa atcagggggt gcaaggaata
     5641 tcttgcattt agttgaatct gagtttgaag aagcgattac tgaagcaata attacttatc
     5701 caaacgcaaa aatattcgat gttaagttga gtgaagccgg agcgtttgaa ggaaaaggtg
     5761 attctaatgc tgaaattatt gcaaagcctt ccgttgctga aagtaaagaa aacaagattg
     5821 tattttcgat tggaggggcg tagctatgaa attcaagaaa tatttactac aaaaccttag
     5881 aataaaacga tgggtcgtaa gaggtctagt tattggagga gtattagcag ttactttagg
     5941 cgctcctttt ggaatgaatg atccaactcc tgacaatcaa gatagcaaac aagaaacagt
     6001 tataaataag aacgttgcat atgcggaaag taaaccggca gaacaaggaa tgtggacact
     6061 atatgctcgt atcctaatgg gagataaaga agttaatgat agtgccgcta aaactggaca
     6121 aatgaatttg ttaggacaag gtggtatcag tgccgatttc ccttattctg acatttctaa
     6181 acaaggtaat tcgattgcag ggggtaatgc aggtactcaa ctagcatcta ctttagcaac
     6241 ttatagttac tacggatata ttgaaacagt ctcaggaaat gctattgcca gtcaagcatc
     6301 atccattcta ggaagtattg gtaggacgat tggtagtttt atcggatcaa ttagtttaat
     6361 cctttctaat attgctgaat ccataaatga actccttgga aaatttttca tgagtatgaa
     6421 tatattttct cttatggggt ggggagaagg tggatcggat gtatctaatc cggtatctaa
     6481 agcattaact agcttgctta acaatttggg attaaataat gaattattta aagcgatagc
     6541 aagcttatct atgctctttt tcacagcaat actaggttat caaggaatga aattattgtc
     6601 gaaagatggt gttaactcga agggattatc accagtactt gaaaaatgga ttgttcgtat
     6661 ttttgtattg tttatattct taccagtggc aggatcgcta ttgggagttt atatcaacgg
     6721 catccagtca gcaaaaaaag atggagatat accaacaaat gttgtttctg actacatcat
     6781 taacacaaga ctttgggctt ctactcaaaa tcttgctcca tcatctgata ctggtggtga
     6841 tgtacctgac ggaaagccta catccgaaat caagttcgtt gatacagatt atgaaccatc
     6901 taaaaaatct gcaatgataa gagcgatcaa tactagcagt tatgccagac aaaataatga
     6961 tctagattct aattcagtcg gtatggctct attaaaagaa tggtcatcga atgatacttt
     7021 caatattaat acttatgccg gtgatgttaa tgtcgccgaa aatgatgctt atatgggaag
     7081 agcgcctgct gtttggacag gctataattt aggggatgga aaagaaggaa gtgcaattaa
     7141 gaaaccaacc aatttaaatg gatatatttg gtcagcaaat caaaatgtga cagacgacaa
     7201 tcgcaagcct gatggaaaag gaacagcgca taactttgat gctactcaac cttggggcgt
     7261 tgctaacaat gcaactttta gcacacagtc ggtagcttta ttgctccaat cggcacttga
     7321 tacatcggga gctaagtttt atgcgtataa tatcgctcct tcaggaattc aaggcagtgc
     7381 taagaatatg tcaacagtaa aaacagaatg gcgtacagca tccttagttg gtcatggagc
     7441 tatgggtaag tttggctcgt ggctagcgat gaacgctgaa tcaatcgttt tagcagttct
     7501 ttacttagca tgtgcttggg cgatgttcaa tgtgaatatc gtgaaagcat ttaaacgtga
     7561 atttattcgg atttgggaag tagttctaac aggatcactt gcatcaggag caggtgtgat
     7621 ggcaatttca ttaggtgttg ttacaacatc tctgatcgga ctacaattac ctatctttct
     7681 gattactttt attcgtgcat ttggtgatac tgctgtggga ttcattccta atacattagt
     7741 tggcgatggc gtagcaaata ttgttcgatc tgctatgatt ttaggtgtag gatatctttt
     7801 agtctttaaa aaaggcgaaa aagtaaataa tttgattaat atgcttattg gcttaccact
     7861 tgaagtagcg tttgggtttg atgaacgtgc taagttatta tttggtaata gaaatgatgc
     7921 tgtgcgagtt gtggacggcg tttctaaagc aatcaagaaa agccaaggag ctaaaaatcc
     7981 ctattatgat ggtggcaaca gctcagtaaa agatatggtt gcaggaggtc ttggagcagt
     8041 tggagcgcaa aaaatggctg aacgtttgaa caatatggga gatagcaatg ctgatgctaa
     8101 gtccattgct gataaagtaa ttcctgaaac accagaagga atgtcattca acggtggcgg
     8161 agttggttcc acaatcaata atacctcttt caaagattta gttcgtcaag atcaactacg
     8221 taataactcc caacaatata ttgatcattt aggacgtatg gttgatgaaa acggaaactt
     8281 tgtcgatgaa aatggtgaaa tattaaaaga tagtaaatac ggtaaagatg ctaaaggcaa
     8341 ctttattgac gaaaaagggc gtttggtcga tgaagatgga aaccttatag atactaatgg
     8401 taaaccattg gaagatagtg aagatggtaa tattggtatg gatagcgaag gacgtttaat
     8461 tgatgaaaaa ggtcgcttag tcgatgaaga tggaaatctt gttgacgaaa atggacgcag
     8521 gttgctaaat ggtgattttg gtcatgatag taacggacgt ttagttgacg aaaaaggtcg
     8581 tttagttgat gaagatggaa atcttgttga tgagtttggt aataaacttg gtaaacacag
     8641 cgatggatat aatgtgaatg gagatttagt cgatgaggat gggaatatca ttgagacgag
     8701 agatgcgctt gtggcagatc gaaatagaga actcgaacaa caacggcata cgccaattgc
     8761 tgttgcaatt cctgagaatg aagaagacga gattacttta gatcaaactc atcaagaact
     8821 gaggaatgaa atgagtggtg aggacgtgtc agacagagtg catcgggaat taggagatgg
     8881 agcatctggt gaaagtcagt caagagagct taacagcaac tttgataata gtgctattcg
     8941 gaatgacaca gtacttaaag acagagaatc gctaacaaaa gaaacggata aagaacaatc
     9001 agatgtgaag aaaaagttca ttcctactcc taatgttaat caaacagata aatatgaaac
     9061 aaatattgga tcaggaatgt cgaaagacaa tcaatcaatt agagaacctg aactgacaaa
     9121 taaaaataat agaacaaata attctaaatc tacttcagat aattcaggga aatctaatca
     9181 atcaaccaag aaaacatcaa cagctaaatc agccgttgaa tatctatcaa aaactggtaa
     9241 aaatgtttac acctcagcag ataaagcttt agcaaaagct ccaaaaggta gtctagcagg
     9301 gataacctat gaagctaaaa acgaagctat taaaataggt aaaggaacgg ctaaggttgc
     9361 caaggctact gctacaggag catccaaaac tggtaaagta gtgaagaaat ctaccatgtc
     9421 agtcaaggac tatattgtaa aatacggtaa tcaaaaatca actgaaaata agtctgaaga
     9481 cgagtaaaaa gaaggagcat tgatttgctc cttcttttta ctttgatttc taactgaaga
     9541 tgtcaaggaa aatttctatt tatgcaaaaa aattatttta aattgtctat aaatgaatta
     9601 aatatttact gttcaaagtt gtataattaa aaagggctta tgctggtaaa gaacagaatt
     9661 attatctaaa caaaaacttg ataaaccagc atttttagcc aataaaaaaa gtgaaataat
     9721 ttaatggaaa atctctt
//
LOCUS       DAZKOB010000101         9736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000101 DAZKOB010000000
VERSION     DAZKOB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.103"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACG8M6_002382"
     CDS             23..646
                     /locus_tag="ACG8M6_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458003.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACG8M6_002383"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACG8M6_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0458004.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACG8M6_002384"
     CDS             2142..3023
                     /locus_tag="ACG8M6_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0458005.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACG8M6_002385"
     CDS             3026..3796
                     /locus_tag="ACG8M6_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0458006.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACG8M6_002386"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACG8M6_002386"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0458007.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACG8M6_002387"
     CDS             5107..6270
                     /locus_tag="ACG8M6_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0458008.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACG8M6_002388"
     CDS             6387..6797
                     /locus_tag="ACG8M6_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0458009.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACG8M6_002389"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACG8M6_002389"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0458010.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACG8M6_002390"
     CDS             9370..9654
                     /locus_tag="ACG8M6_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458011.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3380 a         1649 c         2091 g         2616 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagta
//
LOCUS       DAZKOB010000102         9710 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000102 DAZKOB010000000
VERSION     DAZKOB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.104"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..522
                     /locus_tag="ACG8M6_002391"
     CDS             283..522
                     /locus_tag="ACG8M6_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458012.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="ACG8M6_002392"
     CDS             complement(884..1144)
                     /locus_tag="ACG8M6_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458013.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="ACG8M6_002393"
     CDS             complement(1329..1826)
                     /locus_tag="ACG8M6_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0458014.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACG8M6_002394"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="ACG8M6_002394"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0458015.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACG8M6_002395"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="ACG8M6_002395"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0458016.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="ACG8M6_002396"
     CDS             complement(4548..5210)
                     /locus_tag="ACG8M6_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0458017.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="ACG8M6_002397"
     CDS             complement(5220..6035)
                     /locus_tag="ACG8M6_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0458018.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6297..6740)
                     /locus_tag="ACG8M6_002398"
     CDS             complement(6297..6740)
                     /locus_tag="ACG8M6_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0458019.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6874..7212)
                     /locus_tag="ACG8M6_002399"
     CDS             complement(6874..7212)
                     /locus_tag="ACG8M6_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0458020.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7200..7577)
                     /locus_tag="ACG8M6_002400"
     CDS             complement(7200..7577)
                     /locus_tag="ACG8M6_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0458021.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7801..8994
                     /locus_tag="ACG8M6_002401"
     CDS             7801..8994
                     /locus_tag="ACG8M6_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0458022.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9160..9582)
                     /locus_tag="ACG8M6_002402"
     CDS             complement(9160..9582)
                     /locus_tag="ACG8M6_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458023.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2895 a         1938 c         1591 g         3286 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta
     6181 tttaacaagt tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag
     6241 caagactgtc atacgtctcg cttttgtatt gaaatttctt cgattagaga tacttattac
     6301 tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga
     6361 ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc
     6421 actcgctttg aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc
     6481 tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc
     6541 ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa
     6601 agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt
     6661 tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct
     6721 gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg
     6781 atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac
     6841 ttttctagta atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat
     6901 cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa
     6961 tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt
     7021 gcggttttcg cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg
     7081 gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc
     7141 tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt
     7201 tacttgatac attccatttt atttctagat aacacacgat catatttctt gatttttcct
     7261 tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt tcgtcaaaag ttcactttct
     7321 tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata
     7381 tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc
     7441 cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga
     7501 atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg
     7561 acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta
     7621 ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta
     7681 agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt
     7741 tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa
     7801 atgacaacgt atcagcgaaa ggtattatta tcgacatcgg cgggaattgc tttagagaat
     7861 atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt
     7921 ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt
     7981 ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt
     8041 ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc
     8101 tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt
     8161 tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg
     8221 caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg
     8281 aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc
     8341 ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc
     8401 agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact
     8461 gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga
     8521 ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct
     8581 ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct
     8641 ttgtttttaa tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg
     8701 agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat
     8761 ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt
     8821 atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat
     8881 cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg
     8941 gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg
     9001 agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag
     9061 ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc
     9121 agctttttca actgctaatt tccttctatc tggcggcgac tattctttga cgtccgtcat
     9181 ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg
     9241 agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg
     9301 ataattatat gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg
     9361 cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt
     9421 ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa
     9481 ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat
     9541 tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc
     9601 tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt
     9661 caatagtgca taaacctgtt attatttctc tttttttcct aagtattcta
//
LOCUS       DAZKOB010000103         9594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000103 DAZKOB010000000
VERSION     DAZKOB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.105"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..641)
                     /locus_tag="ACG8M6_002403"
     CDS             complement(<1..641)
                     /locus_tag="ACG8M6_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0458024.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACG8M6_002404"
     CDS             complement(643..1407)
                     /locus_tag="ACG8M6_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0458025.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACG8M6_002405"
     CDS             complement(1415..2092)
                     /locus_tag="ACG8M6_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0458026.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACG8M6_002406"
     CDS             complement(2129..3514)
                     /locus_tag="ACG8M6_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0458027.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="ACG8M6_002407"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8M6_002407"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG8M6_002408"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG8M6_002408"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0458028.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACG8M6_002409"
     CDS             4453..5310
                     /locus_tag="ACG8M6_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458029.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACG8M6_002410"
     CDS             complement(5391..5726)
                     /locus_tag="ACG8M6_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458030.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACG8M6_002411"
     CDS             complement(5793..7163)
                     /locus_tag="ACG8M6_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0458031.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACG8M6_002412"
     CDS             complement(7241..7777)
                     /locus_tag="ACG8M6_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0458032.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="ACG8M6_002413"
     CDS             8086..9567
                     /locus_tag="ACG8M6_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458033.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1970 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt attc
//
LOCUS       DAZKOB010000104         9432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000104 DAZKOB010000000
VERSION     DAZKOB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.106"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..680
                     /locus_tag="ACG8M6_002414"
     CDS             <1..680
                     /locus_tag="ACG8M6_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0458034.1"
                     /translation="YFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHI
                     VQHIGRTFLNHRVKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRAS
                     FKDYLTESEIVDRLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETY
                     TTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRH
                     GKTFLTK"
     gene            800..1000
                     /locus_tag="ACG8M6_002415"
     CDS             800..1000
                     /locus_tag="ACG8M6_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0458035.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1076..1273
                     /locus_tag="ACG8M6_002416"
     CDS             1076..1273
                     /locus_tag="ACG8M6_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458036.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1570..3117)
                     /locus_tag="ACG8M6_002417"
     CDS             complement(1570..3117)
                     /locus_tag="ACG8M6_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0458037.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3201..4004)
                     /locus_tag="ACG8M6_002418"
     CDS             complement(3201..4004)
                     /locus_tag="ACG8M6_002418"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0458038.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG8M6_002419"
     CDS             complement(4007..4789)
                     /gene="phnE"
                     /locus_tag="ACG8M6_002419"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0458039.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG8M6_002420"
     CDS             complement(4786..5565)
                     /gene="phnE"
                     /locus_tag="ACG8M6_002420"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0458040.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG8M6_002421"
     CDS             complement(5537..6328)
                     /gene="phnC"
                     /locus_tag="ACG8M6_002421"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0458041.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6389..7318)
                     /locus_tag="ACG8M6_002422"
     CDS             complement(6389..7318)
                     /locus_tag="ACG8M6_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0458042.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7513..8196
                     /locus_tag="ACG8M6_002423"
     CDS             7513..8196
                     /locus_tag="ACG8M6_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0458043.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8357..9400
                     /locus_tag="ACG8M6_002424"
     CDS             8357..9400
                     /locus_tag="ACG8M6_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0458044.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2893 a         1933 c         1869 g         2737 t
ORIGIN      
        1 gctatttttc tcggttttca ttagcgactc gtgaagccgt tcaatggatt gttattgata
       61 tgtatgcgcc ttatgtttca ttggtgaaaa agcttttccc gaatgctcag ttgatcatcg
      121 accgtttcca catcgtccaa catattggca gaaccttctt gaatcatcga gtgaaagaaa
      181 ccacggcacg gcttaaagga acttcccact ctcaacgagg gttagggaaa aaattgaaac
      241 gctattggaa actcctacaa aaagaagaag caaagttaaa ctacgaaaaa agggtttggc
      301 gtgccagttt taaagattat ttgactgaaa gtgagattgt cgatcgactg ttatctgctt
      361 gtcccaacct gcgccaaggc tatcagttat accaagacat tctttatgcg gtaaagaaaa
      421 gagatcagtc attatttgag gactgtttaa acagagaagt cagcggtctt cctgaaacct
      481 atacaaccac tttacgaacg tttaaaaagt ttctgccaca gatccaaaat gcgcttcact
      541 acagctattc aaatggacca ttggaatgtt taaacaacca catcaaagta ttaaaacgca
      601 atgcgtacgg ctttcgcagc ttttacaatt tcaaactacg gatcatgatt cgtcatggca
      661 aaacattttt aaccaaataa acagaacaga aagagagttt cttctttctg ttctgtccaa
      721 attattctaa gatttctctt taccaacatc atttgacaaa gagcctgtat ttattactgt
      781 cgaggacaat ggcatcggca tgattgaaga ggaacaaaaa aacatttggg atcgctatta
      841 taaagccgat ccgtcaagta aaaatacaaa gataggcgag tcaggtcttg gattatcaat
      901 cgtgaaggaa ctagtgcgcc tgcaccacgg ttcaattgaa gtgaccagcc gaaaaaatga
      961 aggaacgacc ttcctgatac aatttccaaa agaaaaataa aattaccagt aaaaaaccgt
     1021 ggagagatag ctgctattga tttttgctat gtcttttctt tgagaggtat ccggtatggt
     1081 ggagtgtctg aaaaataaaa aaatggaggc caattttgtg aaagctgtta tttctggaag
     1141 tattgttttc caagaagaat atcgagatat ggaaaaagat ttcagtaaaa aaggcattga
     1201 attaattgat tatcctcgct catgtccgat cctagacaaa gagtatccag aaatcttgac
     1261 gacttttttt taaatatcgc agcagcggat ttattttttg tatttaatca agatcggaaa
     1321 ggggtattag gctatattgg tgccgcaact ttttcagaaa tgaagtattg tctgatgcaa
     1381 aatcttattc atggaaaaag gatcaaaata gtcttattta aggaaccatc agaggaaaat
     1441 tttttgtttt gatgaggtag atcgctggct aaagaatggc tggatggaaa tcagacagcc
     1501 taacaatttg gcataacaaa ggtgtgggca aaaaactgct cacacctttt tgaatttttt
     1561 atttatatac tacaaaatca cttgaaaatt atgattcact tttgcttcga tgcgtgggtg
     1621 aatattcaaa tagtcggcaa ttaattcggt catgtcgatt tggatttctc ggacaatttt
     1681 ctccgcacca aacatcgcat aatttcctcc gccgattgcc cgatattgat ttgtcaccac
     1741 ttccagcatt tgttgcggaa gaacggggtg acctttaaaa cgaagttcgg taatccgttg
     1801 tccaacaggc tgtttcatat caatagtgta ctgaatccct tcatacatgt cataattgta
     1861 gtactggggc ttaggctgaa taaaggctgg attgaatacg atttggttat tttctacagc
     1921 taaataattt gcggtctgct ccaacgccgc tctcaaatct gcaccattta tttgaatcac
     1981 tgccagagta ttcggataaa tatagttggt gatgacgtcc cgcatcgtga tcgtctcacc
     2041 aaagcctttt ccttcattat tgaataacgc agttccagaa atctctgagc cgctggcaaa
     2101 taattgtaca cgattaataa actctacata gggatgttcc tcaagacgag cctgcatcgg
     2161 atcattaata cgcatatttc cgttcacttt acctaagggt tgatccaacc atttctctag
     2221 tttttcttct accggtacga tcgctgcaag aatttctgga tcaggctctg atttttcaac
     2281 tgagtgtaaa gcaacctggt gatctgtaac aaccacttgc ccctcttttt tctcgacggt
     2341 aagacgaatt tcaccgatga actcgccacg atagcctggt tgaacaacgg gaactccatg
     2401 aaccgtttca gcaattttcc ggtgctgatg accggtgaaa agggcgtcaa tgccttcaac
     2461 ctgctgcaat agctcgtagc cttcattctc tccagttagc gcttctgtgt cctcaccggt
     2521 taacaagtct ttttcaaatc cgccgtggta actaacgatt accaagtctg cttgtttcct
     2581 taatagagga acatattctt ttgctgtttc gacgatactc ttaaacgtaa ttcctgctaa
     2641 ggtttctggc ttttcccagt gaggaatgta ttgtgtcgtt aatcccaaga tagcaatttt
     2701 tacacccacc ttttcgataa tcttataggc gtttccaaag tatggttggt catttttgtt
     2761 caccatattg gctcctaata taggcgcttg ataattctta atcgcttcct cgagataatc
     2821 cacaccatag ttaaactcat gatttcccaa cacttgaaca tcatagctca tttggttaat
     2881 caagtctgta agctctttgg ctgaaccata ttctttagca agataataac tcaaaggaga
     2941 cccttggatg aaatcaccat tttcgatcgt aattaccggt ccatctgctt ttgcccgtaa
     3001 ttccttcagt ttcgacgata ctttagccgt tcctaaggcc agatcggttc tgccggaata
     3061 gtctgttggt gaaacatagc catgcatatc acttgtctct aaaaacgtaa tgttcattct
     3121 gcgtgctcct ttagctgact caaaatagaa aaaccgaagc gtattcagct gttcggtctc
     3181 tttttttgat ttataatgcc ttaatccttc atttgaagtg tttttaaaat gttgcctgga
     3241 ataaagtttt ctgttgtctg gcgaacaatc cttccttgat cgagatgata aaggttatac
     3301 ccatgacgtt cttcaaagcg tacgaagcca tcgccttcat cttcaccaac aaaggacata
     3361 ctcgatacag taaaataggg gatcccatta atttcatcag taaatgtgtg atgggtatga
     3421 ccgtttagga tacagcgaat atcgtaaggt gcgattagct tcaagaaacg ttcggtacca
     3481 ggccattttg gtatactgct ttggtgagac agtagatgat gatgggtgac caagaaaatc
     3541 ggttggtctt tttcttgctc taaggctttt ttcagccatt gaaattgctg atcgtccact
     3601 ataccatctg cgtatccata gcttgaatta tcaaaggaaa tcactgtaaa ctcaggatat
     3661 ttgactatct ggttgtacgg ggcagaagag gcggcttcat cgcaccagcc tacccaaaaa
     3721 tgttctttga tatcgtgatt gcctagagta acaagaatcg tcgtttctcc tagcgcattt
     3781 tccagccaag tcttcaagta tcgataatcg tctatttcgc catcttcggt taaatctccg
     3841 ctgataatca caaggtccaa gggtgtttgc tgcaggagat gatgaatgca ttgctccaga
     3901 gggataaggg gattctgcat cgtagccagc attcccttat agccttcttc acaagcctca
     3961 tactctctgc gaaagtgtat atctgatata tgcaatatat tcattcttat ctcaatctct
     4021 ttcttataat tccggttatt tgatctaaaa taaacacggt tacgactacg ccaatcaaaa
     4081 taatactcac tttgctccaa gaccgagttt gaatcgcgaa tatcaatgga gcaccgattc
     4141 cgccagcgcc aactaagcct aaggtagccg cactacgaac cgctatttca aaatgattta
     4201 atgctaaaga acaataaatc ggcatcaaat aggggatgcg aacaaagaaa atcgtttgcc
     4261 aaaaatttgc tcctaccgca tgcatttctt caacaagtcg ctcatccatc gcttccattt
     4321 cttctgtaaa aagtttgcca agcattccga tctgatggac gccaatcgcc ataacaccag
     4381 caaaggggcc aggtccgacg actttcacaa aaataattgc aaaaactagt tcaggaaaag
     4441 ctcgtaaaac attacagata agcttgccaa tttttgttac ccatggatgt gcttgccaga
     4501 ggttaaccgc actgatcaag gtaatgggaa tcgctaatac cgtagcgatc attgtcccta
     4561 aacaggcgat tccaatggtt aataatagca aactaatcaa atcctcgccg ctgccatcat
     4621 atagaaactg ccaatcaggc tgacctaacc ctctcaaaac gtccattccc atggaaggag
     4681 agaaggtgct taatccagag taatccagag tagcggccga caaaataacg agaatcacta
     4741 aagcaactaa tgataaaagg gttcttctgt tcatttcacg aagcatcata acagggacct
     4801 cctgacacgt tcacttaatt tatcaacgat cgcgacagtt actaggatcg ttagaataat
     4861 gattgaaact cgttcataat tcaaaagcgc aagtgaggaa tttagaataa cgccaatacc
     4921 accagctccg acatatccaa ggacagtaga agcacgaaca ttcacctcaa acgcgtaaaa
     4981 agcatagctc gcaaactgac tcaaaacttg tggagcgatc gaccaaatag cgatttgcat
     5041 tttatttgct ccaacagccg cagcggcttc aatcgggcca aagtcaatgg tttcaattgc
     5101 ttgaaacatt aattgtgaca ccataccaaa agtaaagaca gcgatagtca acacgccagt
     5161 cgcttcacca attccaaaca cagcaacaaa aagcgcggct aaaagcaggg tagggatggt
     5221 ccgtactaaa cctaaaagaa aacgaaagat ggtagaaaga agacggtttt ctgtcactac
     5281 agtggtcgct aaaaaactaa cagggatcgc taaaattaag ccaatgaccg ttccaagcaa
     5341 agacatttga agtgttttca ccataggctc gactaattta ggtaaatagc taaagtctgg
     5401 atgaatgaag cgtgataaaa agacagtcat ttgattggag ttttgaaaaa cctgagaaaa
     5461 atcagctgac gtgatccagg cacttccttg aaaacaaaca attaagaaca gaagaataag
     5521 caggtgttta taccatttaa agtgaacggt ttctttcaag ctcatcgatt tcgcctgcct
     5581 tttcttcaaa gattgcattg ccataaattt gctctaagcg ttcatcggtt aagtcgtctg
     5641 gacgaccatc aaagacaacc tgagcagctt ttaacgcgag cacacgcgta gaaaactcac
     5701 gagccagagg gactgaatgc agattgacaa tgactgtttt atccatcgtt tgattgatat
     5761 tttttaggtc catcataacc ttttgagtcg tgattggatc taatgatgca acgggttcat
     5821 ccgctaaaat aatgggggct tgctgcacga gtgctcgcgc aatggatact cgctgttgtt
     5881 gcccaccact gagctcatca ctgcgtgtct gcaatttatc agacaaacca actttttcca
     5941 gtgcttcctt tgtctgctgg tagtcttcct tcgtaaaaag acctaaaata cttttccaag
     6001 tggagtaata gcccaaacgt ccagatagca catttttctg aaccgtacta cgctttacca
     6061 gattgaagct ttgcgagatt agaccaatct ggcggcgaat ctgtcgcagc tcttttttat
     6121 tcgcttttgt aatagaggtt ccattaatgc gaatatctcc tttggtggtt tctgccaagc
     6181 gattgatact gcgcagcaga gtgctctttc cagcaccact caaccctatg atggaaacaa
     6241 attctccatc ttcaattgtt aagtcaatat ccttcaagcc aacaacgcca ttgggataga
     6301 ccttcgaaac attgtcaaaa ataatcatta tccttctcca tttttttaag tgtaagcaaa
     6361 aaaaagcttg ctgacactta ctgatgtttt attctgctgc ttttttcgtg tagtctctga
     6421 tcgtgtcata ggcagaatca gcagcttctt catagccctg atgcccccac aaagccattg
     6481 cttctttgcc atctttatca tccttcatat ttaagaaggt ctctttaatt ttcgctttta
     6541 aatcttttga catgtcaggc tgtacagcga ttgcgtcatt cggaatatcg ccttttgtca
     6601 ggtacaggac ttttaaatcc ttaaataaat cattatctgg gaaattcgaa gcaaaaacat
     6661 ttcgcgcgcc ttcaaaaaca aacgccgcat ccatttgctt gttcaacact gcggtaatct
     6721 cactcggaat atcattcaca gtggtcatct ttgtatctgc agtaggatca atcccagcat
     6781 ctttcaattc agcgacagga tagatgtagc cgctagcaga gttaggactt aaggttgcga
     6841 tattttttcc ttttaaatca gttaacttat tcaatccgct atcggcacga accaatactt
     6901 ctcctttaaa ggtgttcgta agtttgtcct ttaatggcaa gccagtttct tgatcataat
     6961 cgccgagctg agaagtcagg attgcatctg cagctccagc atcctttgct tgaacatagg
     7021 ctgcaggcgg cataattccg acatcgactt gaccagaaga catcgcttcg acgatcgttg
     7081 aataatcagt agctaacgtc acttctacct cacgatctaa ctttttcgat aaatacttag
     7141 caaatggttt tgcttttgcc tccatactac catcattgtt tgttggaacg aattgaacaa
     7201 ctaatttttc tttgctctct ttttctgagc tgctacttgt attcccagaa ccgcaggccg
     7261 ccaaagtggt caataacatg actccgatga acccttttgc taactgtttg aactgcattt
     7321 ttctcctcct gttggatata atttctaata cgtctaaatc atagcaggga atcaaaatca
     7381 atgaagtgac gcacgtccta ttttacacaa cttttacata caatttacaa acaattataa
     7441 ataattattc gtattgagcc gaaaaacaat ctaagctata ataaagaaaa acttcttaga
     7501 ggtgacgatt tcatgaaaaa aatccatgat acgaagcaat taactgctta cgttgagaag
     7561 cacaacctgc aggattatat ggattcagat atattggcaa tggcttcttt actttttttt
     7621 gaaaaggaag agcacctcat tcaaacggga aaaacctcag attatttgta ttttttagtg
     7681 gatgggacgg taatggtgta ctcgtattct tctgatacac aaaatatttg catcgattac
     7741 gcaaagcccg ctacaccact cggcgaagcc tcttcattgt gggaattatc gccgaaaagc
     7801 agcgttaaag cagtgactaa gtgcatctgt atcagcattc cgctaaatca atatcgaaca
     7861 cagcttcaaa aagatgttcg ctttttgcag aatatttgcc agttactaag ttatcgttta
     7921 aattccggga taaatttggc gaactcactg acagaacctg ttgaaactcg tttagcaaag
     7981 tttattttga cccatcacga aaacgaacgt ttctcatttc ggttaaccac ctgcgcctca
     8041 atcttaaatg tcagttatcg acatctttta cgaacaatca cgaattttcg tgaggagaat
     8101 attatcgaaa agaaaaagaa ttattacttg attcatgata tcgaagcttt agaggactta
     8161 gctgagaata taagtagtcc agtaaaagca gaataacagc tataaaaaaa cagcagtcag
     8221 aagtgattgc tggttttttg atagtggaga aattgggaaa cggaaaatct tcgttgaaga
     8281 gtagtgttct atatattggt atgctatact tttaaaagtt caatgtgtca agacacataa
     8341 aaaggggaga aaaattttga agaaaatact tgtttcattg ttgtttttgg tgtcattagt
     8401 aacgttagtc ggctgtagtc agtctcaggg ggagaagcaa agtgaaaaag agaaaagtaa
     8461 aatcgctttg gttcttggat ttggcggggt tgatgaccgc tccttcaatc aagctggctg
     8521 ggaaggatta gaggcttggg gaaaagagaa aaagctgaca gcaaaaaaag attttacgta
     8581 tatagaaacg aacaatccag aggataacag taccaatatc gatcaggcaa tcagaaacgg
     8641 ctttaccacc attattagct tgagctactt gcaagcagag gaactggaaa atgctgctaa
     8701 agataatcca aagttgaaat ttgccttggt agatggtgag attactggga aaaagaatat
     8761 ctcatcagca acctttaaag atcaagaaag tgcgtattta gcaggactag ctgccgcata
     8821 tacgacaaaa agtgatcaag taggctttat tggtggaatg gaagggcccg tgatccaacg
     8881 tttccaaagc ggatttgaag ctggcgttaa ggcaggggcc acagcactga acaaggaaat
     8941 tactgttaac gtacaatatg ctgcatccta tgattcgcta gataaaggaa aagctattgc
     9001 tgcttctatg tacaaagagg gagtcgatat tctttatcag gcggcaggaa gtactggagc
     9061 tggggtgttc caagaagcaa aagcccgcaa tgaaaacgac tatagcagaa aagtatgggt
     9121 gattggtgta gatcgcgatc aagcggatga aggggattat aaaatggaga acggcgagac
     9181 agggaatttc acgttgactt ctacaaagaa aaatgtcggt gcagaagtca aagaatttgc
     9241 agaaacttct cataacgata aatttcctgg cggcaagcat ttgacctttg gtttgaaaga
     9301 agaaggggta gatctgacaa aaggacaatt atcagaggaa gcctatcagg cagtttcaga
     9361 cgcaaaagag aaaatcatca gtggtgattt cagactgtag acaaagtcca ttttatggat
     9421 gatgtctaca gt
//
LOCUS       DAZKOB010000105         9376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000105 DAZKOB010000000
VERSION     DAZKOB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.107"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1178
                     /locus_tag="ACG8M6_002425"
     CDS             24..1178
                     /locus_tag="ACG8M6_002425"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0458045.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1200..1952)
                     /locus_tag="ACG8M6_002426"
     CDS             complement(1200..1952)
                     /locus_tag="ACG8M6_002426"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0458046.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2316..4982)
                     /locus_tag="ACG8M6_002427"
     CDS             complement(2316..4982)
                     /locus_tag="ACG8M6_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0458047.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5323..6774
                     /gene="cls"
                     /locus_tag="ACG8M6_002428"
     CDS             5323..6774
                     /gene="cls"
                     /locus_tag="ACG8M6_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0458048.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7103..7918)
                     /gene="thiD"
                     /locus_tag="ACG8M6_002429"
     CDS             complement(7103..7918)
                     /gene="thiD"
                     /locus_tag="ACG8M6_002429"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0458049.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8212..8487
                     /locus_tag="ACG8M6_002430"
     CDS             8212..8487
                     /locus_tag="ACG8M6_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458050.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            8914..9369
                     /locus_tag="ACG8M6_002431"
     CDS             8914..9369
                     /locus_tag="ACG8M6_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0458051.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         2836 a         1903 c         1637 g         3000 t
ORIGIN      
        1 atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc ttttttattc
       61 ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat cctgcaaaat
      121 accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa gacatcatta
      181 cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag ctaatcgaca
      241 tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg gtaattatcc
      301 accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag gcttgctacg
      361 gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca gaacgaaaag
      421 tactagtcat agcaagtgat attgctcgtt acagcttggc aagcggtggt gaagtgacgc
      481 aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta tcgattgaag
      541 acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat tatagcgaat
      601 ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc caaaaagttt
      661 ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg attgctttcc
      721 atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac caaacagacg
      781 aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac agccgacgaa
      841 tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg gaaaattcga
      901 aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt gcagtaagtg
      961 aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca caatctcatc
     1021 aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg attttcagtg
     1081 agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct ttttcgatta
     1141 caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa aagaaccctt
     1201 cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc
     1261 ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca
     1321 acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc
     1381 tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt
     1441 tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc
     1501 ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat cacgcaccat
     1561 ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct
     1621 gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg
     1681 ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt
     1741 cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatctg
     1801 ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga
     1861 taatttttta catcttcagc aatagttact tgcgattctt gctggatttc ataatattct
     1921 tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg
     1981 ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct tttgatgaat
     2041 aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt
     2101 gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca
     2161 aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca
     2221 agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga
     2281 attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca
     2341 atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta
     2401 aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc
     2461 attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag
     2521 aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc
     2581 cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg
     2641 ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa
     2701 atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta
     2761 gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg
     2821 atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg
     2881 atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta
     2941 tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc
     3001 aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg
     3061 tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga
     3121 cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa
     3181 acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc
     3241 ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct
     3301 gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt
     3361 accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc
     3421 aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct
     3481 agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat
     3541 tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg
     3601 atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg tccagtagac
     3661 attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt gtttcggatt
     3721 tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg
     3781 cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc
     3841 aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca
     3901 ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct
     3961 gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca
     4021 tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat
     4081 gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct
     4141 gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc
     4201 aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc cagacctcgt
     4261 tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc
     4321 gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac gatcgttcca
     4381 ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct
     4441 ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca
     4501 tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct
     4561 accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg
     4621 ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca
     4681 ctgattatag aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc
     4741 atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt
     4801 tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct
     4861 ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc
     4921 tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc
     4981 aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aagagaccta
     5041 agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg
     5101 ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt
     5161 caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta
     5221 cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat
     5281 catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata
     5341 accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga
     5401 gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt
     5461 gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa
     5521 agtgacttga aagaattaga aaactttcag tcttttagag gagataccat tgaacattac
     5581 gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc ttcattaaat
     5641 cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg
     5701 gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca
     5761 tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa
     5821 ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa agcaaaagat
     5881 ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaagggca
     5941 ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag
     6001 attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc
     6061 ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt
     6121 cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg
     6181 aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag
     6241 cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg
     6301 attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt
     6361 gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc
     6421 aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc
     6481 aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata
     6541 atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg
     6601 aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag
     6661 ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct
     6721 aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt
     6781 tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag
     6841 tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
     6901 caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc tggacggcag
     6961 gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac
     7021 caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg atgcagggca
     7081 actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga ttgatggggc
     7141 catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag tcttttgcca
     7201 atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata catgcagata
     7261 gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt aaccaaaatg
     7321 attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca tttttagcat
     7381 gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt gctgcttgca
     7441 caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca taaaaattag
     7501 gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa gcagattctt
     7561 ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat ggtccaaaat
     7621 catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct agcatacctg
     7681 ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg tcgataaaat
     7741 ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca catgcggtca
     7801 tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct tgcatccctg
     7861 cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga tcttccatca
     7921 aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt ttctatcata
     7981 tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa tcaaatttat
     8041 ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat tcgctattct
     8101 aataataaat aaccactatt tgatttatat atatttcagt atccttcttg ttttcatata
     8161 accaaaggcg ctatgatttc catatgaaaa aaataaagtc aggtgaaaaa aatgaaactt
     8221 gaggataaaa aagtcatttt gaagaaaatc aaacgactac cagaccaaat agatggctat
     8281 acgatctgtt ctttcaaaaa agacaggagt ataaaagctt atacgcaaaa cacagaaaaa
     8341 ggaataatct atttcctaca tgaagaaggt tacgataccc aaacctttat gtttactgaa
     8401 aaaaaagaat ttcataaaca gatgaaaaaa ctgattgaaa aagaatttcc aagaagtcat
     8461 aaactctatg tgaatcagca attttaactt ctaactgttc tttattaatt aaaaatgttc
     8521 tgtaataaaa taaaacttta taacaactat aacaaaaccg aatgcaaaac agaagttcta
     8581 taagttataa tcatagttgt taggtgattc atctagtaaa aacaccagat ttcaaatttc
     8641 acacaattta aactagatca atcatcaacc ttcttcaaat tgttagaacc ctttcccgag
     8701 gagctgcagc ataagcaaaa aaacaaacag gaagccaaat ggctaagaca ttttttctat
     8761 tagtctagtt gcttctcgtc gcaagtttaa gactttagaa agttatttct aatgatgttt
     8821 gggttgtttt gatcaatgct tatgcacagc tccttaccta ttatttatct caacagatct
     8881 aacaaattaa tggactattt aaggagtttt tttatgctta cgatctattc ttctcgcaca
     8941 catagttacc atctgatggc aaactggctg aaagaccatc aaatgcctta cagagaacga
     9001 attttcacac ctaaacagcc tttaacaagg aaagagatat tttatttatt gtcattaaca
     9061 gaaaacggat ttgacgatct attagctaaa cgctcgattg attataaaaa tttgaaagac
     9121 caaatcgacc aatgtacgac ctcagagctt gtcgatctga tcgtctcgca ttttactttg
     9181 ttacaacgtc ccatcattac tgacggaaag aaattattag tcggattcaa tgaagatagc
     9241 atgcgtcttt ttatccctgt acaatggcgc agaagtcaac tgaaatctgt ctataacgat
     9301 tttacatggg taagcaatct agaaaattct acggatttag atgatgaatt tttaaattat
     9361 tatatataaa aaagac
//
LOCUS       DAZKOB010000106         9258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000106 DAZKOB010000000
VERSION     DAZKOB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.108"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..657)
                     /locus_tag="ACG8M6_002432"
     CDS             complement(<1..657)
                     /locus_tag="ACG8M6_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0458052.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     Q"
     gene            791..1078
                     /locus_tag="ACG8M6_002433"
     CDS             791..1078
                     /locus_tag="ACG8M6_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458053.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1494..3032
                     /locus_tag="ACG8M6_002434"
     CDS             1494..3032
                     /locus_tag="ACG8M6_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0458054.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3029..3994
                     /locus_tag="ACG8M6_002435"
     CDS             3029..3994
                     /locus_tag="ACG8M6_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0458055.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3981..5303
                     /locus_tag="ACG8M6_002436"
     CDS             3981..5303
                     /locus_tag="ACG8M6_002436"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0458056.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5366..5470
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5544..6287
                     /locus_tag="ACG8M6_002437"
     CDS             5544..6287
                     /locus_tag="ACG8M6_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0458057.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6302..7294
                     /locus_tag="ACG8M6_002438"
     CDS             6302..7294
                     /locus_tag="ACG8M6_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0458058.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7306..8043
                     /locus_tag="ACG8M6_002439"
     CDS             7306..8043
                     /locus_tag="ACG8M6_002439"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0458059.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8033..8755
                     /locus_tag="ACG8M6_002440"
     CDS             8033..8755
                     /locus_tag="ACG8M6_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0458060.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8839..>9258)
                     /locus_tag="ACG8M6_002441"
     CDS             complement(8839..>9258)
                     /locus_tag="ACG8M6_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0458061.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3026 a         1600 c         1959 g         2673 t
ORIGIN      
        1 ctgttcaaga ggatcaagtg gttcaaatgt ttctgtagtc tgttttgctt ccaattgggg
       61 caaaacagat ccaatttcca gatacttcac tttattcaat gtataagata gaatagaaca
      121 gctaagtaat gtatacagaa aatgtgtgag atccaaataa agcaaggaga caactaaaat
      181 accaaaagta atcaagaaaa gacttttgcc aatcttaata tgaaaatatt tttcaatggc
      241 ttgataaatg atatctgttc cgcctgtgct tccgccgaat cgaaagacga gaccgcaacc
      301 aatgccagaa agaatacctg tcatcaaact attgatcagt agactatcta cagcaaaatg
      361 tatatgaagg ctctcccata aatgtaaaaa gaaaactagc gcaaatgttc cgacgatcgt
      421 ccggagaaaa gcgtgttttc caaaaagaaa caatgagata agcaataaag gtaaattaaa
      481 aagcagtgaa gtgacagaag gagcgaatcc aaaaagatta tgcaaaagca aagcaatacc
      541 agtaacccca ccatctgcca gcttagatgg aatattgatt cctgtcacag ctaatccgta
      601 gattcctgcg cctaaaatga ttccgcacaa ttctgtaaat aaatttaatt ttttcatttc
      661 tagcctcttt tcttttgaaa attttcagca acttctttat tatactgact tatactctta
      721 cttcaagagg aaaatgaaaa aaaattattt ttggctgttt ctttttattt atcaaataag
      781 gagtggaaaa atgcgagcag cagacttatt gacaattctt acaaaatcac ccaaaaaaac
      841 agagtatcaa ggagtttttt tggctcaaga acaaaaactg gtgcctgtga caatggtcca
      901 gataaataaa gcaaatcaat ttatcttttt atttgaacac ggcaaagcac cacttgctat
      961 gaaagacatc tttctttttt tgatgaagaa ccgtgagaaa gctgtttatt atcagtcaaa
     1021 agaagaactc attcctcttt atggagtgaa agagatagaa aataaagtgg tgatataagt
     1081 aaaaataaga aaacaagacg tttatgatgt caaaacaacc attttttaca tttttggcaa
     1141 aaaagtgtca tgatacagta taatattcgt tgaacaggcc aataaaggtg ttctccccca
     1201 cgagatttct actttattgg ctgtttcttt ttattaaaat aaaagttgtg tgtgtaaaaa
     1261 agatatagct gattccaagt gacaatttcg tatagatttt tatggtattc gtagtgaaaa
     1321 aacgagaacc aaaaagttga tccgactttg ttgcttggat tctttttttg tcagcatatt
     1381 tagaggtatt tttttctttt ttttgttcgt gctgtaaggt taaggcaaga caagttagtt
     1441 gggaaaaagg aaagaaatgt ttatacttaa agaataacag aaaggaaggt cgcatgaata
     1501 attttataca aacattaagt gaaagaaaag gcgagttgct ggaagctgca tttcaacatc
     1561 tgtccatttc tctcatttct ttgctgattg cagcactgat tgctattcct ttagcgattt
     1621 gggcagccaa tcataaaaaa atggcagaag tcttattgca gatcacgggt gtgctccaaa
     1681 cgattccttc acttgcctta ttaggtttac ttatcccatt tgtagggatc ggaactgttc
     1741 cagcattgat tgcacttgtt atttacgctt tgcttccgat tttccaaaac acgtatattg
     1801 gtctagctga aattgacccg tctattgaag aagcagcggt agcatttggt atgtccagaa
     1861 tgagacgctt gctgaaagta gagcttccca tcgcattacc tgtcattatc tcaggtatcc
     1921 gaactgcctt agttttaatc atcggtacgg ctactttggc agcattgatc ggagcaggtg
     1981 gattagggac atttattttg ttaggtattg accgaaatac acctgttttg acattgattg
     2041 gtgcgatcag ttctgccttg cttgcaatta tattcagcgg actgattcgc tggttgcagc
     2101 ataagaaacc tcgatattcc atcattactt tagcagcagt ggtgtgttta tttggcggcg
     2161 tttctttata tcagtctgcc ttgtttaaaa atgaaaccat cacgattgct ggaaaattag
     2221 gagcggaacc tgaaatcctg atccaaatgt ataaagaatt gattgaagac aacacaaaaa
     2281 cagaagttga attaaaaccg aattttggca agacgagttt tttgttcaat gcactggatc
     2341 aaggtcaaat cgatatctat cctgagttca caggtactgt cctagaaagc ttggtgaagg
     2401 tacctgaagc aatgaaaagt gctgatttga caaaagaaaa aacatatgac gaagcagaag
     2461 aattattgaa taagcaattc gatatgacat tattaaaacc aatggcttac caaaatacgt
     2521 atgccttggc agtcaagaga tcttttgcga acgaacatca acttaaaaag atttccgatt
     2581 tggcagctat ctctgatcag atcaaagcgg gattcacact ggaatttatt gatcgttcag
     2641 atggatataa aggaatccaa gaagcatatg gcttgaattt ttcaagtgta caaagcatgg
     2701 agccaagttt gcgttaccaa gcaatcaata atggagatgt caatattgtg gatgcctatt
     2761 ccacagatag ccagctcaag gagtacgatc tagtgactct agaagatgat cgagggttat
     2821 tcccagctta tcaaggcgca ccgctgatga aaaaagattt tgctaaagaa catcctgaaa
     2881 tcgttaaagc actgaataaa ttaagcggaa aaataacaga ggatcaaatg attgatatga
     2941 attaccaagt caatgtcgaa aagaaaaagc cgacagaagt agcacgaatc ttcctgaaaa
     3001 atgaaggatt agtaaaggag gaagatcagt gactaaatta atcgagttca aacatgtgca
     3061 aaaaaagtat gatggaaagt atgtcatcga tgacctaaat ctggcaatca aaaaaggaga
     3121 aatctttgtt ttggttggtc cttccggtag tggaaaaaca actactttga aaatgatcaa
     3181 tggattatct aaaccatcag caggagacat ctacttcaaa ggaaaatcgt taaatgaata
     3241 taatctgcag aaaatgcggt ggaatatggg atatgtgctg cagcaaatcg ctttgttccc
     3301 gaccatgaca gtcaagcaaa atattgaagt catccctgaa atgttaggtt gggaaaagca
     3361 gaaaagagca gaccgtgtag atgaacttct tcaaaaagtt ggcttatctc ctgatattta
     3421 tcgagatcgt atgcctagag agttgtctgg aggagaacag caacggatcg ggatcatccg
     3481 agctattgct gcaagcccag atgtgatttt gatggacgag ccatttagcg cattagatcc
     3541 gatttcaaga aactcattac aagaattggt tttgtcctta cacgaagaat tgggcacgac
     3601 catcgttttc gttacgcaca atatggaaga agcaatcaag ctaggtgatc gtattgcatt
     3661 tatgaaggat ggagaaatta tccaatgtga cacaccagag cagttattga tgaatcctaa
     3721 aaacgactac gttcggcatt tctttgatga accgaaacag acgaaagagt ggcgagtaga
     3781 agatttagtc gtcaatggat atttcttaaa tgaaatacca gaaaatgctc gtcaacaagt
     3841 ttgctttgat acgcctatga aagaagtata ttcgcttctt tcggtgtatc caagcattgt
     3901 gatagtagaa aagcaaagaa gtattgggtc gctgacaagt aaagaaatct ttgctttttt
     3961 aagtcgagag gaggaaggaa atgaaaacat atgatgcaat catcatcggt tcaggtgtga
     4021 gcggcttaag tgctgcttat ggattgaaag aagcaggtaa aacagtacta gtggtcgaag
     4081 aagatctatg gggcgggact tgtcctaacc gtggatgtga tcctaaaaaa gtcttgttaa
     4141 gtgcagtaga ggcacgtaat cgggtaaaac aattgagtgg aaaaggtttt aacgagatcc
     4201 caactgccaa ctgggaagaa cttcaaaaat tcaaacgaac atttacggat ccagtcccag
     4261 aaagccgaaa aaaacaatta gcagaagctg aaatcgatca tctttcagga accgctcgtt
     4321 ttttggatga ttcatctatt gaagtcaacg aagaagtctt ccatgcggat tacttagttt
     4381 tggcaaccgg gcaaagacct acgatcttac cagtggaggg aaaggaatac ttgaaaacta
     4441 gtgcagactt tttatcattg cctgtactgc ctaaagaaat catttttatc ggtggtggtt
     4501 atattgcttt tgagctagct acgattgcca atgctgcagg gagcaaagta accattgtcc
     4561 atcataatca acggccatta aaagaatttg aggcgtcttt ggtagaggaa gccgtccatc
     4621 aaatggaagc atctggcatt cagtttgctt ttggtgttga aacccaaaag atcatttcag
     4681 aaggtactcg ctatcgttta gtcggaaaag agacggaatt agtagcagat atgatttttt
     4741 gtgcgactgg gagacagccg aatacagaat ctttagcact tgagcaagcg aatatcgtct
     4801 ttgataaaca cggaattgca gtcaatgatt atctgcaaac gagcaatcca aaaatttttg
     4861 cgtgtggaga tattgtttca aggaaaaccc caaaattgac accagtcgcc acttttgaag
     4921 gaaattatgt agctaagcgg ataacggatg caacaagcga acctatcaaa tatccgatta
     4981 tacctacgat cgtctacgcc agtcctaaac ttgctgaagt aggagttaca aaaagtcacg
     5041 cctcttcgtc agatcaagtg gtagagatgg atcttaccag ttggttcacc tatcatcgtg
     5101 tcaatgaacc agttgctaag gctgaattga cctttgacca gcaaaattat ttgattggtg
     5161 ctgcagtgat tagcgagcaa gcagacgaat tgatcgatga tttgacactc gtcatcaatc
     5221 aaaaattgac taaaaaagag ttggatagct atatcatggg gtatccgaca ttggcaagtg
     5281 atctctccta tttattgaaa taatcagcat gataactaaa gcgcttgttg ctttttcctt
     5341 cttgtgctac actttaaaaa atgaatacct gtttctgggg atgtcgtaga cgacatgaga
     5401 gtaagttgtg aacttagacc cattgaacct gtcagttaat actggtgaag ggagaaacat
     5461 agcagacgct tgaagcgggt tactatgtcg tgcccatttc aagcgttttt tgttattcat
     5521 attagaagaa aggaggtaac aaaatgaaga aaaatcttta tccagcagtc agtatggcat
     5581 tcattttaat tatttggcag attgttgtca tagtttggaa aatacctgcc tttattttac
     5641 ccggacctgc tgctgtattt tctgcattag cggcagatgc acaagtatta ctttcccatt
     5701 ctgtcacgac attaaatgaa gcagtgcttg gtttggtaat agctagcgtt ctttctttta
     5761 ttactgcctt ggcaatggat tattggaagt ttttagaaat gtctttttat ccgttactag
     5821 tgatttcaca aatgttgcca atcatggtgt tagggccatt acttacccta tggtttggtt
     5881 tcggtatgct tccaaaagtg atccttgttg tcttgataag ctattttccg atcgtagttt
     5941 ctttttcgga tacattgagg aaagtatcaa aagagcaaat catctttttg aaaacgatgg
     6001 gggcagatac agggaagatt tatcgaatat ataaaatacc agcgggttta tccggcttct
     6061 tttccggttt aaaagtggct gctacttatt gtgtcagcgg agcagtcgtt ggagaatggc
     6121 taagcgctca agcaggctta gggtattaca tgatacgcgt aaaaaacagc taccagatcg
     6181 ataaagtttt tgctgcgatc atttgtgtca ttctattgag tttattgctg aatggcgctt
     6241 gttcattatt gaaaaaaggg tattatcgta tgctttatat tttttaagag aaggggatca
     6301 aatgaaaaaa ttatggcttt tattaccgct actgttgctt ttatccgctt gtggcactgc
     6361 aaaagaaaca agtagcaaac aagaaataaa ggatttgaaa aaagtgacac ttgttttgga
     6421 ttatgtaccg aatactaatc atactggcat ctacctagca aaagaaaaag gctattataa
     6481 agaggctggt ttaaatgtcc aaatcatcga accaggagat aattcgacaa gtattggtct
     6541 agtaggtgcg gacaaagcac aatttggtgt aagttatcaa gaagatgtca cttatgctca
     6601 cgcagatgga cagaatatac cagtcaaagc aattgctaca gtgatcaaac ataatacttc
     6661 tggattcgcc accctatcag acagtaacat ccattcacca aaagattttg aaggaaaaac
     6721 atatgcaggt tggcaaagtc caagtgaaga agctgtctta aaagctgtga tggaaaaaga
     6781 cggcggggac ttttcaaaac tgacaatggt aggaagcaat ggagaagggc cagaaagctt
     6841 aggaaaatcc tcagatatcc aatggtactt tgaaggttgg gatatgatca aagcaaaaga
     6901 agcgggtatt gaactaaact atatcccatt gaaagaatta gatgaacggt tagattacta
     6961 tactccggta attattacga atgatcaact gatcaaaagt gatccagaat tggtacagtc
     7021 ttttatggat gccacgaaaa aaggctatca ggaagctatt aaggatccaa atgacagtgc
     7081 taaactgttg caaaaatatg caaaagaaaa tgatcggacg tttttagaag aatcacaagc
     7141 attcttgtca aaaaattata cggatgatcc tgagaattgg ggattgatgg aagaaaaagt
     7201 ttggcgcaat tatacagctt ttatgcagga aaacggattg atcaagcaag atgtaccttc
     7261 gcaagaacta ttcactaatc agtttataaa ataggagtga tagatatgtc aatccaaatc
     7321 agccacttga atgtagtcat tgaaaacctg aaaatattag aagacatcca tttcactatc
     7381 cctaaagata cctttacatc gattgtcgcg ccaagcggag ccggtaagtc aacattgctg
     7441 aaaactttaa caggagtcca gcctatgact tctggaacga tcaaagtaga cgatcaaaaa
     7501 gtaactggtc tccatacagc atttagttat atgccgcaag aagatatgtt acttccttgg
     7561 ctgagcgtct atcaaaacgt tacgttatat caaaaaatca atcatctaac aatcaatgaa
     7621 gaacaagtaa gggattatct ggatatcttt gggttaaacg gctatgaaca ttttttgccg
     7681 gaacaattat ctggcgggat gaagcagcga acggccttgt tacgaacaat catgaatcct
     7741 tcttcttact tgcttcttga tgaaccattt ggagcgttag atgctatgac cagagggcag
     7801 atgcaagact ggatgttgaa gctgcctaaa aaagcaaagc ggactacttt acttgtgacg
     7861 catgatatcg aagaagcaat ttatttatca gacaggattc ttgtgctttc cgcccgtccg
     7921 gcacatgtga ttgctgagat tcaagtgcct gaaaaacgac ggtcacgtga atggctactg
     7981 cagcaatcag agttgaagca aacgatttat cagttattgg caggtgaatt agatgttaag
     8041 tgatgctcat tgtcatttag atgggagtca gagtttagca ctccttcagc aggaacacac
     8101 gattttgacg atcatcaatt gtgattcgcc agaagaatgg aaggaaaaca gacagcttgc
     8161 agcaagtaag actcaagcct taagttacgg tatccatcct tggaaagcag atagctatac
     8221 gtttgaacaa gttgagcctt ttttgaaaaa agcaaggatc atcggtgaaa tagggttaga
     8281 taatatttgg acgaatgtac cgatgactac acaaaaaaaa gtatttgagc gacagcttgc
     8341 ttttgcagcc ataaatgaga aacctgttgt actgcacaca aaaggatgcg aaaaagagat
     8401 cttaaattat atccaagctt accctaatca ctatttgatc cattggtatt cttctttgga
     8461 ctatcaacaa gactatattg atttaggctg ttatttcagc attggtcttg atttgaaaaa
     8521 gaatccagca gtctggcaac tagctaaagc agtcccaatc gaccacttac ttattgaaac
     8581 ggatggacta catgcagcag agtgggcttt aggggggatt ttttcagaaa aagattatgt
     8641 ctcactgctt gaagaacagt taaagacaat cgctgagatc aaagagctat cagttgaaga
     8701 agttaaaaaa aatacttatc aaaacttgga acgtttcatt agattaggta aataaaaaag
     8761 agatgtgaca caactagtgt cgcacctctt tttggctttc tttgtataaa agtaaactgt
     8821 ttgattaata aatgaatctt agtaccaacc gtttgcttcc cagaatgctt tcgccgcttc
     8881 ccaagaacca taacgtgaag taacatattg ttctgctact ctttcttggt ttgcagcaga
     8941 gtagtcacca tttaagtaag aagaatctaa ttggtaacgt ccgatgtaac gtccgttcgt
     9001 tgctgtgtaa gaaccgcttg attctttttg tgcgatccat tcttttgcag agcttgtgct
     9061 tgctggagta gcagcagttt ctgttacagc aggagcaggt tgtgtttcag caacaggcgc
     9121 tgcttgtttt tccgtttgaa ccgtttcttg tacaacaggt tgttcagctt ggactggtgc
     9181 ttcttgaacg gctgcttgag gttcagcagc tgcttgtgca gagccttctg ttgggattgt
     9241 taattgttgt cctgagta
//
LOCUS       DAZKOB010000107         9247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000107 DAZKOB010000000
VERSION     DAZKOB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.109"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..2680
                     /gene="clpB"
                     /locus_tag="ACG8M6_002442"
     CDS             71..2680
                     /gene="clpB"
                     /locus_tag="ACG8M6_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0458062.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2831..3517)
                     /locus_tag="ACG8M6_002443"
     CDS             complement(2831..3517)
                     /locus_tag="ACG8M6_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458063.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3495..3989)
                     /locus_tag="ACG8M6_002444"
     CDS             complement(3495..3989)
                     /locus_tag="ACG8M6_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0458064.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4009..5229)
                     /locus_tag="ACG8M6_002445"
     CDS             complement(4009..5229)
                     /locus_tag="ACG8M6_002445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0458065.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5376..7211
                     /gene="lepA"
                     /locus_tag="ACG8M6_002446"
     CDS             5376..7211
                     /gene="lepA"
                     /locus_tag="ACG8M6_002446"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0458066.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7407..8318
                     /locus_tag="ACG8M6_002447"
     CDS             7407..8318
                     /locus_tag="ACG8M6_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458067.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT         3145 a         1602 c         1880 g         2620 t
ORIGIN      
        1 gcgatataat agtttttgta aagatcaaag aaataagtga tcttaccttt aattaggagg
       61 gtatacctgt atgaatattg agaaaatgac tacgacgctt caagaagcga ttgctgaagc
      121 tcaacagatt gcggtcactc gaaaacatca agatatcgac attgcccatg tatggaaaat
      181 ctttttacaa tctaatcatt ttggtcgaaa tttctatacg gatgccggat tggatgtcga
      241 atcttttgaa catgaaattg atcgtctact ggatgagtat ccagttgttt ctggtggaaa
      301 cgtccaatat ggacaaaacc tcagccagaa tttctttcga ttattgaatg aagcagacca
      361 aatacgtgaa agcttcggcg acgagtttct ttcaacggaa gtcgttattt tagcgttgat
      421 gaaattaaaa aattatccgt taactgttta tttgaataaa aacggattat ctgaaaaaga
      481 attgcgtaaa aacatcgaag agatgagagg aggagaaaga gtgacctcac aaaaccaaga
      541 agaacaatac aaagcattag aaaaatacgg cgttgattta gtacagcaag tcaaaagcgg
      601 aaaaatggat ccaatcatcg gtcgtgatga agaaatccgt gatgtcatcc gtattctttc
      661 acgaaaaaca aaaaataatc ctgttctgat aggagaaccc ggtgtaggga aaacggcaat
      721 cgttgaaggg cttgctcaac gtatcgtacg caaagatgtg cctgaaaatt taaaagataa
      781 aacgattttt tcattagata tgggtgcatt gatcgctgga gcaaaattcc gtggtgaatt
      841 tgaagaaaga ctaaaagcag tcttgaaaga agtcaaaaaa agtgatggac gtatcctctt
      901 gttcatcgat gaaatccaca atatcgtagg ggcaggaaaa acagaaggaa gtatggatgc
      961 cggaaatctg ttgaaaccaa tgcttgctcg cggtgaatta catttgatcg gtgccacaac
     1021 tttagatgag taccgtcaat atatggaaaa agacaaagcg ttggaacgtc gctttcaaaa
     1081 ggtattagtc aaagaaccaa cagtggaaga tacgatcagt attctgcgtg gattaaaaga
     1141 acgttttgag atccaccatg gtgttaacat ccatgacaac gcattagtag cagcagctac
     1201 tttatctgac cgttatatta ctgatcggtt cttaccggac aaagcaatcg atttgatcga
     1261 tgaagccagt gcaacgattc gggtagaaat gaactctatg ccaacggaat tagatcaagt
     1321 tactagacgt ttgatgcagt tggaaatcga agaagctgca ctgaaaaaag aatcagatga
     1381 tgcaagtaaa aaacgcttga aaaatttgca agaagaatta gctgaacttc gtgaagaagc
     1441 aaatgcgatg aagatgcagt gggaaacaga aaaagaagaa gtcaattctg tttcagcaaa
     1501 acgtgctgaa atcgataaag caaaacacga attagaagat gcagaaaata actatgattt
     1561 agaacgcgct gctgtattgc gtcatggtac gatacctcaa ttagaaaaag aattgaagga
     1621 attagaagct aaagcaaaag acagcgagat taagatggtt caagagtccg ttaccgaaaa
     1681 tgagattgct caagtcgttg gcagattaac gggtatcccc gtaacgaaac ttgtagaagg
     1741 tgaacgagaa aaactaatta aattgaatga aacacttcat aaacgagtga ttggtcaaga
     1801 tgaagcagtt gatgcggtca gtgacgcggt aattcgttca cgggctggat tgcaagaccc
     1861 aaatcgtcct ctaggttctt tccttttcct aggaccaact ggtgtcggaa aaaccgaatt
     1921 agcgaaagcg ttagctgaga acttgtttga ctcagaagat catatggtac ggatcgatat
     1981 gagtgaatat atggaaaaac atgctgtgtc tcgtctagtc ggagctcctc caggctatgt
     2041 aggttacgaa gaaggtggtc aattaacgga agctgtcaga cgaaatcctt atacaatcgt
     2101 cttgttggat gaaatcgaaa aagcgcatcc cgatgtcttc aatatcttgt tacaagtcct
     2161 agatgacgga cgcttgactg attcaaaagg gcgagtagta gatttcaaaa atacggttct
     2221 gatcatgacc agcaatatcg gttcacagtt actgctggaa ggcgtgactg ctgatggaac
     2281 gattccagaa gcagtggctg aacaagtcaa cacgctatta cgtggaaact tcaaaccaga
     2341 attcttgaac cgaatcgacg acacgatttt atttacacca ttgagcctag acaacgtgaa
     2401 aggaatcgtt gacaaaatgg tggcgcaatt ggcacaacgt ctggaacatc aagaaatcct
     2461 gctgacaatc agtgatgaag ccaaaacgtg gattgctgaa aatgcttatg aaccagcata
     2521 tggtgcaaga ccattgaaac gatttatcac taaggaagta gaaacaccac tagctaaaga
     2581 aatcgttgca ggacatgtta tgcctaaatc aaaagtaacg attactttac ttgatggaca
     2641 acttcatttc aaaacagaag agctagaaga aatcgtctaa tcttccattg agactataaa
     2701 agaaaaaagg ggtctgacac aactagtgtc agacctcctt ttttcaaata aacagtgttc
     2761 cagaagtagt ttcttcgaaa aataagccga aatttcttga agctgcccgc ttctgtcaca
     2821 acctctttta ttaggtattt ttcacttttt ctaatttata actttcaaag taacgctcat
     2881 cttttttact ttgagaccat gcaaacaatc ccattaaggc agcacctatc gtatttgtca
     2941 gcaagtcgcc catcgtatcc atcaatgctg ctcgtccgac aaacagtgtg ccatcagaag
     3001 cagcaaaacg ttgaagattc atgcctaaga actggtcgca taagaattcc cagaactccc
     3061 aaaacacgcc gcaaagtcca gcaaacgcaa agccgaataa gaggaacaac caaggactgg
     3121 tttttgatat ctcagcatct ttcatcaagt aaccaatcaa tccatagcca agagctgtca
     3181 ggaccattgg actcaccgca tgtaagattt tgtcccaaaa agagatgata ctgatcaaat
     3241 gcatccctgt gcctaaaaag acagagatga acaagaaaaa ccagtaaaac aaaacgattg
     3301 ctgctggtat tttaagtttg aaaactttca aaatgacttg tggcatgaaa atcaatgcaa
     3361 tccctaataa agattccacg ataaatgtga ttccttgaga agtcgaatat ttttgtgtga
     3421 taaattcata aaaattatag cagacaacga atacaccaaa agcaagtaaa gctatttgca
     3481 cctttttgat ttctttaatc actgaaagtc cctccatcta aaaatctttt tacttctgaa
     3541 agcaactcaa tttctgaaga aaccactttt ccgttcaatt tgatcaaacc tattgtatat
     3601 aagttcaggt agtgaaattg attttcagct gtttcttgta gtgcagcgat ttttttcggg
     3661 tgggttccgc cttgctgtcg agtatcagta tataagccga tcactgggat gcctttcgca
     3721 taagccacac cgatttccga tgctaccccg gcatcgatcg tcagcccatc tagtaaagca
     3781 atcattaaat cactagccaa cactttttct gtgtcagcaa gagcaatcat tcgactgtct
     3841 gcataagcag ttttgtcatt gattgcttca ttttcttgag gaagatagat cgaaagctca
     3901 gaagagagct ctctgatttc tttaactaac tgttggttat ataacaaatc acttttagca
     3961 aacattggtg cggcaaaata aattttcatc taaacatcat cctttttatc aaaaataatc
     4021 gctaaagtaa gcttccatgt ttggaatgat acgctctagt gacttcagtg tttcttcatc
     4081 tgtttctaac cgttcgattc ttgccaaata agaagcacgg cgataattca tcatcaagca
     4141 agtcaatgtt tggatatcca gtcgaacgga ttttcctata ggttcattta agacacggac
     4201 ttgtccctgt tcatcccatg ttaaagcgaa gacaccatta ttccaaggag cgacaggatc
     4261 ttcaatgatg aaatgaaaag catcagctgt tccaacaaac ggaaaccgtt gaaggaattc
     4321 ttttacatca acgatccgag ccataaaata tggttcgatc tgctctttga tctgactatc
     4381 ttcaatcaaa aatgctaaag gctcattttt ataaatatct ccatgtaccc agtagatcat
     4441 ggaaaaatgt gcggaaataa agttccataa gccatttcga gcttcttgat caagatagaa
     4501 catttctttt acgtgaaata tttcttcagc tacccagtag aacagaacac ctcttggttc
     4561 atggttgcta tcgtagtaga cagcggctgt gcgctcatct tcattttcga aacgccaata
     4621 ctcttcccaa tgaaaatcac tgcggaataa agcaccatgg ttttgttggg caaatttgtc
     4681 ataaacggaa aatacgtctg gatgatcaac tggtaatcgc tcaaccatac cacttactgg
     4741 gatttgttta ggtagctgtg tatctctgat tttaaaggaa agtttatcag acatgatttc
     4801 ccatccttta cgccgatagt aggggatact gtaaggatac aaataagaga tccattgctt
     4861 attcttgcgc atattatcca atgcaacgat aattagatct tgcatcaatc catggttagc
     4921 atattctgga taagttccaa ctccagtcac accacccatt ttataacgtg caccgtgaat
     4981 attgacttca caaggataga tagcgatttg agagatcagt ttattttcgt ggaaccatcc
     5041 gaacactttc gatacttcca aaatcggttt tttggaacgg ataaaagctc gtttattctc
     5101 aaacccgctt tcctcgatgt ctgcttcagt cacttggaac acataggaaa gcaattcatt
     5161 gaattgatcg atatgttctt gagtgaccgg ttttaattca agattttctc taaaatcttt
     5221 ttgatccatt ttttcatctc catttattag cttgataaca gtatagcaaa attctacaaa
     5281 aaaaacttct atagctgact aattaaaaac tctttaaatt actgcttttc gttggtataa
     5341 tggaaagtgc tgattatatg aaaagaggag acgctatgga tataaagaaa atgaaagaac
     5401 gccaagagca gattcgtaat ttttcgatca tcgctcatat cgaccatggg aaatctacat
     5461 tggccgatcg tattttagaa atgacgcaca ctgtgacgtc tcgcgaaatg caagatcagc
     5521 tgttagactc gatggatttg gaacgtgaac gtggaatcac gattaaatta aatgctgtag
     5581 aactcaatta cacagcgaaa aatggagaaa catatatctt tcacttgatt gacaccccag
     5641 ggcacgtcga cttcacctat gaagtctctc gaagccttgc tgcctgtgag ggtgccgttt
     5701 tagtggtcga tgcagcacaa ggaatcgaag cacaaacatt ggccaatgtt tatttagcgt
     5761 tagacaatga tttggagatc ctgccagtca tcaataaaat cgacctaccc gcagcagatc
     5821 ccgaacgtgt ccgtcaagaa atcgaagacg taatcggaat cgatgctagt gaagctgtat
     5881 tagccagtgc caaatcaggg attgggattg aagatattct cgagcagatc gtggaatatg
     5941 ttcctgcacc aagtggagac ttagatgcac cgctaaaagc tttgatcttt gactctattt
     6001 atgatagtta ccgcggtgtt gttctaaatg tccggatcac ggacggtatg gtcaaacccg
     6061 gagacaaaat caaactgatg agtaatggca aaacatttga tgttacagag gtcggggtct
     6121 tctcgccaaa agcagttgct agagactttt tgatggtcgg tgatgtcggt tatatcacag
     6181 caagtatcaa aactgtccaa gatacacgag taggggatac agtaacgtta gcagacaatc
     6241 ctgccgctga agctttacca ggttaccgaa aaatgaatcc gatggtttat tgtggtcttt
     6301 atccaattga tacttctcgt tacaacgatt tgcgggaagc tttagaaaaa ctgcaattga
     6361 atgatgctgc tttacaattc gaaccagaaa cctctcaagc attaggtttt gggttccgct
     6421 gtggattcct cggacttctt catatggatg tcgtccaaga acgcttggaa cgggaattca
     6481 atctagaatt gatcacgact gcaccatccg ttatttacca tgttaataaa acggatggta
     6541 cgactgctac agtagataac ccggcagatt ttcctgaacc tgtaacgatt caggatgtag
     6601 aagaaccatt cgtcaaagca cagatcatgg tcccaaatga ttttgtcggt gcagtaatgg
     6661 aactttctca acgaaaacgg ggagaattca ttacgatgga ttatttagat gattaccgag
     6721 taaatgtcgt ctataatatt ccgctttctg aaattgtctt tgactttttc gacaaattga
     6781 aatcaagcac gaaaggctat gcctcattgg attatgaaat gtctggttat caaaaaagca
     6841 agttagtgaa gatggacatc ttgctgaatg gagaaaaagt cgatgcatta agctttatcg
     6901 ttcaccgaga ctttgcctat gaacgtggaa aagcaatcgt tgaaaaactg aaaaaactaa
     6961 tcccacgcca acaatttgag gtgccaatcc aagcagctat tggacaaaaa atcgttgccc
     7021 gttcagacat caaagccttg cgtaaaaacg tcttggctaa atgttatggt ggagatgttt
     7081 ctcgtaaacg taaactctta gagaaacaaa aagaagggaa gaagcggatg aaacaaatcg
     7141 gctccgtcga agttccgcaa gaagccttca tggccgttct gaaaatggat gagcaagata
     7201 gtgtgaaata acttataatc aatcgaaaaa cctattaaat aaaccttttt gagtagatgc
     7261 aacactgagg attatctgta taaaagattt taaactgtca tttggttatc acggcgatac
     7321 taaaattaac aaaattggaa tatttttctt gaagattttt agctgtcagt atatgatgaa
     7381 agcatctcat aggaggtgct tttttcatgg ttgataattc aagtggaagg gttcttaaat
     7441 ctctaagaaa agagaaaaaa ctatctcaaa aaaagttagc tgatttagca ggtatttcac
     7501 aaagtacttt agttaaatat gaaaaagggt caagaaaaat ccctaaagac gtagataata
     7561 ctctaagcaa gattctgaat atagaaactc taataaaaga tgaggaagat aagattgaga
     7621 ttctcatcgg taaactaatt gcatacagag atatgaataa gttacttaac aaagagttag
     7681 ctgataatat aggaattagt gaagtattac tcagttatgt gttaaataga aagagaaatc
     7741 cgtcaaaaga aatgcaaaaa aaaatcgata tttttctatt aagtaatgaa aaagaaattt
     7801 tgaaggagat taatcgtgat agtgagatat ttagtttatc aaaagacgat aaaattgtta
     7861 tgggaaaaag aatacgagaa gtccgaaaga accgggaaga gacgcttgaa aaatttggta
     7921 aaaactttac catctataca ggcaaaaatg ttataagtag atgggaaaaa ggtattaata
     7981 ttccagatat tgaaaaacta atgaatattg cctatcttgg taaggtcact gttccttacc
     8041 ttatgtacgg agaagattat aagaatatac ttccaaaaga tgaaagagtt agcgatttta
     8101 aaaagataaa ttcttttagt atgggattaa ggatgaggaa aattaggaaa gattattatc
     8161 tagaaaggga ggagtttgga aagctttttt ctccttcaat ttcaaaatgg tccatagata
     8221 ggtatgagaa tggtagagat atacccaata ctaatcgaat tatccaatat gcttatatag
     8281 gaaatctttc tttagaattt ttgatttacg ggatataaaa atagtttacg tcgatactta
     8341 ttaagttatc gacgtttttt attatagaaa aaatttataa aaggaggttt cttgatatga
     8401 taagaaacaa aaagggtttg ttgaaagttg ctttactggc agcttttttc taagtaactt
     8461 tttgacaagc tcaatggcaa cacaagcgga agaagtaata caagaactaa ctgttatgaa
     8521 taacagctag ataaaatggg ctatgtatgt tatccaacaa tttagtaatc actagaagcc
     8581 tagcgaaagt tcgcttgata agttatagat gagtattgat ttgtagtgtt tccaaatcaa
     8641 aagggtacag agccacatga aatatgttgt cctctgcagc gtcaggatgg gaatccatac
     8701 catggagatt cggagatctg tgagggaata cttacgtggt taatatgtca gttgtgttgg
     8761 acgtagtaat ggtcatagtg ggtagattga ctgaattctt atatgagaaa atgaaaccaa
     8821 caagaagata gggttattta tacagaattg gggatctttt ctggaaaagt aatcggataa
     8881 tgcgagattc gtacggtctt ttttaaattt ctctaaaaaa aacggcatcg aatgggttgg
     8941 gtgattgata agcaatgaat catagactga tatagaataa aaaatgattc tatttatctt
     9001 tttacatcta tcgttttttc aagtagccgc aaattatatc agcattcaac aatttttgta
     9061 tacaagttct agtaaaagga tctgaagaaa aagttggttg ttgtgaagat aaaaaaagaa
     9121 tcaggtttta ctgagttttt cttcaaaatt tgtgttatta ggttaggaca aagataagaa
     9181 gaagtatgaa gatttaaaaa aggaatgagg aaaagatagt aatttccgat tattggagtt
     9241 tttttat
//
LOCUS       DAZKOB010000108         8961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000108 DAZKOB010000000
VERSION     DAZKOB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.110"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..303
                     /locus_tag="ACG8M6_002448"
     CDS             4..303
                     /locus_tag="ACG8M6_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458068.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(400..834)
                     /locus_tag="ACG8M6_002449"
     CDS             complement(400..834)
                     /locus_tag="ACG8M6_002449"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0458069.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1066..1776)
                     /locus_tag="ACG8M6_002450"
     CDS             complement(1066..1776)
                     /locus_tag="ACG8M6_002450"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0458070.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1766..2626)
                     /locus_tag="ACG8M6_002451"
     CDS             complement(1766..2626)
                     /locus_tag="ACG8M6_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0458071.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2631..3269)
                     /locus_tag="ACG8M6_002452"
     CDS             complement(2631..3269)
                     /locus_tag="ACG8M6_002452"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0458072.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3284..3583)
                     /locus_tag="ACG8M6_002453"
     CDS             complement(3284..3583)
                     /locus_tag="ACG8M6_002453"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0458073.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3626..5110)
                     /locus_tag="ACG8M6_002454"
     CDS             complement(3626..5110)
                     /locus_tag="ACG8M6_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0458074.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5130..5591)
                     /locus_tag="ACG8M6_002455"
     CDS             complement(5130..5591)
                     /locus_tag="ACG8M6_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0458075.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5609..6676)
                     /gene="ulaG"
                     /locus_tag="ACG8M6_002456"
     CDS             complement(5609..6676)
                     /gene="ulaG"
                     /locus_tag="ACG8M6_002456"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0458076.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6894..7664
                     /locus_tag="ACG8M6_002457"
     CDS             6894..7664
                     /locus_tag="ACG8M6_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0458077.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7937..8764)
                     /locus_tag="ACG8M6_002458"
     CDS             complement(7937..8764)
                     /locus_tag="ACG8M6_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458078.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2726 a         1879 c         1398 g         2958 t
ORIGIN      
        1 aacatggatt atactgttga atttgacagt gaactactta agcaatttcc acaagaagaa
       61 catttcatgt ataaagaagc aatttcccac aaagtgacag agtatttgga gcataatttt
      121 tttcgtataa agccagtgcg tactcgttcg gcaaaaggaa tatacgagat gaaagtcaaa
      181 attaataaaa aaaattatcg catagcattt gctttaaaaa aaaatcatgt gttcattttt
      241 tatattagtt cgaatttgca aaaaattgta tttgataaag aggtgagtaa aaaaataaaa
      301 tagctgagca ttggaggata gattgagtga tactaagtct atcctcttgt ttttctaaag
      361 aactaagcta gaattttttc ttttgtacgt tattatatgt tatttgtcat taccaaaaag
      421 cacctttttt cttctagtat ctatgacata agaaatacca cccatttcct ttacttgaac
      481 aaatcctact gattcataaa atttttttgt tttctcagta tcatctgtca ataattcgat
      541 ttggactggt ccataactag tgatcttctc caaagcttca ttcaaaagca ctttgcctat
      601 tccttggcgc tgatacctcg gatgaatgag gatatcttga ataaatgcga tcgtcgcaaa
      661 atcagtgatc caacgaacga ttccgataat cttcccttcc ccattacgct ttatcagaga
      721 ttcagattgt tcaaatgctc tagctaacct tgcagtgtct tttgtgtaat tcgtccaacc
      781 aacgctctta tataattcag aaatctcatc gatcataagg gaatcaatat tcattttttt
      841 cttcctttct tatacaattc tccctctaat aatacagttc tttgactatt taatctatca
      901 catataactc tcctgtaaag attctctcga ttccttaaaa ttcctctttc cttttagaat
      961 gcatactttt tgataaataa aaaggttgtg ataaattttc ttcacaacct ctgtttctct
     1021 ctatcaagtg ttatctttta attttctacc cttaccctag catctctatt gcccgtagta
     1081 tgcatacttt ccatgtttgc gaaaataatg tttatctagt aaatattgcg ggatttcaca
     1141 tatattgggg ttgatcagct cgttttcttt ggccatcaga caaatttcat ctaagatcaa
     1201 actgttttct actgctttca taggtgaatc cccccatgtg aacggtccat gtccatagac
     1261 aagtactcca gggacttcgt ttggatctaa ttttctttcc ttaaacgtct cgacaatcac
     1321 attccctgta tgaacttcgt aagcttctct tacttcttct ttcgttaatt ggcgtgtaca
     1381 aggaacctta ccataaaaag catcagcatg agtcgtgcca taagctggca aatcgcgtcc
     1441 tgcttgcgcc cacatgacgg agtgcgtgga atgagtatga gtaatagcag ttatatcttc
     1501 aaaagtttga tacaaaacaa catgtgtggg gagatctgaa gatggtttta agctatcttc
     1561 ttctattata tttcccgaca aatctgttac caccatttga tctgcttgca tgcattcata
     1621 tctgacaccg cttggcttaa tgacgatgac tcctagctca cggtccactt cactcacatt
     1681 gccccaagtc aatttgacta atccgtatcg cggcaatgcc agatttgctt caaggacacg
     1741 ttgcttcatt gctgcaattt tttcttcaca agacatcata gccagcctcc ttcaatttag
     1801 aacttaagta gtcttttgcc tgctggattt ctcttcgaaa gtcttggctt ttttcactcc
     1861 acatttcaat cagaaatgtt ccatcataat ctagacgttt caaatgtctt aataatccaa
     1921 gaaaatcaac acaaccgcta ccaaaaggaa catcacgaaa ctggcctgaa tattcttttg
     1981 ttacagggta agtatctttt aagtggatag cagtgatata atcgatccct ttctcaagtt
     2041 ctcgagccgg attattttcc ggccatgcgg acaaattgcc taggtctgga tatacttgta
     2101 gaaaaggtga atgaatctgt tctttgattt ctaaaaattt tgaaatggag ttcataaatg
     2161 gatcatccat gatttccacg gaaagaatga tgccatattt agaagcttcc tttacgcttt
     2221 cttttattcc ttcgataaag tcttctctgg aattcattga tttctcttca taataaacat
     2281 cgtatccagc tatttggatt actttgacgc ttagattatg cgccaaatgg attgcttttt
     2341 tcattagttg tttcgctgct tttttctttt ctggatcagc agaaccaaac ggaaatcgcc
     2401 gatgcgcact taaacaaatc gaatggatac gtacaccgga gctgaacatc tcttctctca
     2461 gctcagctat cttttcttca gaccaatcta atcgtgctaa ccgctcatcc gtctcatcaa
     2521 tcgacatctc gataaaatcg aagtccattt cttttacaag ggaaaaacgt tctttccatg
     2581 aaatatcttt gggtaacgct ttttcataaa tcccaatttg agccatttcc ttacccccag
     2641 atacgaatga tctcatcttt gaagttttgt gcggccttca tcggatcatt tgctgctgta
     2701 ataccacgac cagtgataaa ggtatacaca tcgatccctt caaataagtg taatgtgtgt
     2761 ggatttaatc caccagttac agaaacatta aagcctagtt caatcagttt tttgatcttc
     2821 gataaatctt tttcacccca agtttcacca gataacagcg catcacggct ttgatggtag
     2881 attacctgcc ggataccgat gttatgccaa tccatggctt gttcaaaagt ccaattgccg
     2941 tagagttcta cttgcaattc acctatttct ttttgtgctg cctccatagt tggaatcgtt
     3001 gcacaacaaa tgactgtcat ccagtctgct cctgcttttg caacattact cgccactgtt
     3061 cctccagcgt ctgcacattt cgtatcagcg actagttttt tatctgggaa catactgcga
     3121 atacagcgaa tcgctttttg tccttcctgt aagcaaagaa ttgtgcccac ttcaataata
     3181 tctactacat caccaacttt cataacatct cctaatgcat gttctaagtc attgtgatct
     3241 aacgcaattt gcaaattcgg cctgctcatc ttagttcctt cctttacttg tttttttggt
     3301 accactttgt ttcagcaaat ttttcactga tttctttgac tgacatcaca tttttgatac
     3361 cgataactgt aactcctttt ttctctgcct gagtaaacat attcaaaaaa ttgactggcg
     3421 taaaaactac atcgtattgg ccagctgaac tttttccctc tgaaatggca caatgatgga
     3481 ttttggtaat tggtacgttt agttctttca ttgctttttc cacacttctc atcatcatca
     3541 agcttgttcc tgatccattt gcacacgata ctaaaattct catcttaatc cctccattat
     3601 gagtgttgtc taatcagatt ttcttttata ctctctgctt ttctgcatat gtgtcatagt
     3661 cttctacaat caaaaaataa ccatcaggat ttttccgata ttgtaactgc ggaatagcca
     3721 ataaaccaat gacaactacg gcaattccag cataacctag gactttcatg attactgtaa
     3781 acaatggcca agctgttgcc cagtcgaaca tcccgatata accaccgtat gttgacatcc
     3841 caatccatgt agcgaataat gctgaaccgc ctacttgtat gattcctgaa agaaacggga
     3901 acaggcaagc ggctttgaat ccacctcggt tattggcata aactgctata acggcattat
     3961 caaagaacaa cggaatgaaa ccggctatga cgatcgttgg tgatttcaat aaaatcagta
     4021 aaccaatcat caaaaactgt cccaaagcac caaacaagac cccaatcgtt accgcatttg
     4081 gcgaaccaaa gccaaaagta gcagctacgt caattcctgg aacagctcct ggtaaaatcg
     4141 tgtttgagat tccttggaaa gattgagtta gttcatcgac gaatgtccga acacctaatt
     4201 gcaagatcgc cagatatacc gcaaaattta aagaagtcgt cataatgtag aataagaaac
     4261 tctgcccttc ttgcatgaat tgcgcttgaa tcaaataatc tttgcctagt acaatcagaa
     4321 tgatcccaaa gaaaaatagc atgagtaacg aagtagcaac catattctca ttgaaaatcg
     4381 acaagaatcc tggtaatttg atatcttcaa tttttttgtc ttgcttgatt gatctatttt
     4441 tcttttcatc ccgctttttc atccactcag ctaattttgc aaatataaag attccgaaca
     4501 tttgctgatg tgccactgca aatccggcac cttctgtcaa ttcctgcgtg attcctactg
     4561 ttaagttaga agctactgtc caataacaac ctaaaatcag tcccataaaa atcaatgctt
     4621 gtatcctacc tagatcagga aagcaaaaca gtaaaatcca aaaagcggta gctgcttgtt
     4681 gaacctgaac gtttccggta gtgaaaacgg aacgcagttt cgtatatttc tgaaaacgga
     4741 ctaataagat atttaaaaca aatgcaatca gtaataaaat catcacatct ccaaaggtac
     4801 ggccaaatgt ctccattaat ccagcatcca cagcattttg tccaaaataa ggatctgtaa
     4861 ccatggcatc caaattgaac cgttccttta gtccaaccaa gattggccga aaattattga
     4921 ccaaaccacc agagccaaca gtcaaaataa agtaaccgac cgttgccttt aagaatccag
     4981 cgatgcattc atgaagcggg cgtttcagca aaatatagcc taataacacg ataaaaccaa
     5041 tcaggtatgc tggctgcgtc aagatatttg ttgcaaaata cgtccaaatg cttaacaaaa
     5101 attcccccat cttttttctc ctctatcaat taattatatt ctgccatcac aacttggtaa
     5161 tcatcgatgg agttcgtaga gatgagacgc tcaaccaatc catctgtcat taataattcg
     5221 gataattttt ggatattttc tagatgttct tctggatctt ttgctgcgag tgtaaaaaac
     5281 aaactagccg ttttctcctc attctcccct tcactttcaa aacggattgg ctgtttcaac
     5341 ttggtaaatg aaatagctgt tccaaaaaca ccatcacttt tttctgaaga atgcggcatt
     5401 gctacttgag gtgcgatcac aatataagga ccgtattttt ctacacaatg gatgatctcc
     5461 tccacatacg tttcatcaat aatttcctta tcgatcaata caagacagct ttcggcgata
     5521 gcttcacgcc aatccgtgat ttctttatct gggtaatgaa tcaattgatg gtcaaaaaag
     5581 tatttcagca agaaactccc cttctttctt ataaaaacga cggaaacggt gtatcatttt
     5641 caatcgagaa gacatcttca aatcctcgat aaaaattaaa ctcaagatca tctttgtttg
     5701 tcggatagat gaatttcccg cctacttgcc agatataagg tttaaatcca tacttcaggc
     5761 gatccttttt gaatttccaa atttcagtga tctcttttgg atcagccata aaattagacc
     5821 aaatatcgta gtggacagga atcactactt ttgcattcaa tgactccgcc atgcgcagca
     5881 tatcaacaga agtaactttg tctgttatcc ctcgtgggtt ttcaccatag gcccctaaac
     5941 agacatcgat tttgtgttca ttaccatgtt tggcaaataa attggagtaa tgtgaatctc
     6001 ctgcatgata aagattccct cctgatgttt cgaataaata gtttactgca atttgatcca
     6061 tctcctgagg catttttcct tttaatacct cttctggatc ttcacaagtg accaacgctg
     6121 tccgatcaaa cgcttctaaa gctactacat tgatgtcttt gattttgact gcatcacctg
     6181 gctttactgt aatcgttttt tcttcaggaa ttccccattt ttgccacgtg cgaaccactt
     6241 cttttggccc aatgaatgtt gcttcaggac aattttgatg aactgctgcg gctgtatgga
     6301 tatctagatg atcggaatgg atatgtgtaa cgattaaagc gtctacattt tttatcgcaa
     6361 aaggatcgat tacgaaaggc tgagtacgta aattaggctg catattttga acgccactca
     6421 tacgcatcat ctgatggcct tttttcatca tgccattccc atgactacgt tttcccgtcc
     6481 cacaccatag atcacataaa atattcgttc cttcatgact tttcagccaa attcccgtac
     6541 agcccagcca ccacatagca aaagttccag gatccactac ttctgtttcg atttcttcat
     6601 tcaagtatgt gccccactca ggaaatgttt ctactaacca cttttctttt gttacttcat
     6661 tgacagtttt actcatttgc ttgctccccc catatcgttg ttccaactta caagtaaata
     6721 ttaatgtgta tcagcatcca tgaaaaccct tttatatgac cgtttgtttt ttatatgatt
     6781 ttctttcagt aatcaatgat tgtttcagta taataaagac agatatgaat gtttaaatga
     6841 acaactagtc aaataaatcc atggatcaag atgtccctca ggaggcaaaa gtcatgaaaa
     6901 actcacttgt caatattgaa aaacgtcaaa aagaaatttt ggaactgctt cagcagactg
     6961 aacaactatc aactttcgaa ttagctgaat cccttgatgt atctattagt acgatacgaa
     7021 gagatttaaa tttattagaa gaaaaaaacg agatcatacg caattacggt tattgttctt
     7081 ataattacaa aaaccagact gattttgatc aatctggtcc tgaaaggatt aaacaagcga
     7141 ttgccagaga agctagtaaa tacattaacg attatgatac gctgtttatc aattctagct
     7201 cgactgcgtt gaaaacactg gattttcttc aatctaacca tttgacagtc attacaaaca
     7261 acgtgaaaat caattactct ccgcacaaag ataattgtag ttatatttta actggtggag
     7321 agatgcgctt tccaaaagaa gcactagtgg gcgacatcgc attaaatacg atttcttcag
     7381 tcaatgcaga catttgtatc attggctgta gcggagtcga tgtagtacat ggggtcacaa
     7441 caaagatatt aaatgaatca acgatcaacg aaatgatggt caacaagaca atcaaatgta
     7501 agattttagt cgctgatcat cgaaaaatcg gactgacatc aaagttcaag attgctgata
     7561 tttcagtctt tgattatttg atcactgata aattttgttc tgaaaaagta ttagaggaga
     7621 tcagaaatac ggggataaaa accattcaag tagattcatt ttaagaacca ggacgtaaat
     7681 atgtaaatag gattatcaaa aaaatgaata acaaatttat cgataaatga aaaacacgct
     7741 acgctcaaat tgataaagga atcccaagat gtatccatcc aatcgatacc atcataatct
     7801 attgcttctt ttataaccgt tatatgttgc ctaaatagga atcaaacaat atcgaaaaaa
     7861 ggaggtatga caaaagtcac acctcctttt ttcgaataaa cggtattcaa atcagaacac
     7921 tgatttcaca accactttat ttcaggtcgt tttcaatcac ctttaccgcg taatcatttc
     7981 cgttgaaacg cgctattttt tttgcgtcca tatatctttc taaataatca tcttctccta
     8041 attgcttatg gcaagaagca atttcataaa gcaagtattc caatgcataa gtcgatttat
     8101 gtttcttatt atactcaata cccttctcac ataattggat tgctttatca taatcctcga
     8161 ttttacggta ataggtggcg atatcataat atacaatgca gaccatcaga ttatcaaaat
     8221 cagtctcact caatatttta agtgtacgct ccacataaac tttcgcaaaa tctaattctc
     8281 cttttgacaa gtagatcatt gccacgtttg cagctgcttg taagtgatgg atatctgttt
     8341 cggtcgtttg ttccaacact tgattaaaat aaaataatgc tgcatctggt tttttcgcat
     8401 aaagatgatc aagcagtcct tgataaaata aaatttgatt taaaagatca gaattactta
     8461 ctttttcctg atcgatcgtt tgtaaaattt tataagattc ttggtatttt gatacagaat
     8521 tcaatgattg aatctcattt atttttttcc tgattttgtc ttcttctgtt tctactaata
     8581 tatcggagat ctcgacattc agctttttgc aaagcgcact taagatattg atgcctggcg
     8641 acatattctt gttttcgata ttgctgacag ttgcttgttc acaaatgcct tctgctaatt
     8701 gtacttgtgt taaccccttt ttctttcggt attcttttag taatgctcca tctactttaa
     8761 tcaaaacaat ttccttccct ttaaaaaatt atttctcctt tatatcatac acaaaaaaaa
     8821 gttctaaaca accaaaaatg tttagaacag aataaaaatt ttaattttaa aataatattc
     8881 aaattaaaca actggcgtat ctgctccttg atttcttttt gtgttatctg aataattatc
     8941 gtttacagtt ataacacata g
//
LOCUS       DAZKOB010000109         8942 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000109 DAZKOB010000000
VERSION     DAZKOB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.111"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..260
                     /locus_tag="ACG8M6_002459"
     CDS             <1..260
                     /locus_tag="ACG8M6_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458079.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="ACG8M6_002460"
                     /pseudo
     CDS             467..667
                     /locus_tag="ACG8M6_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="ACG8M6_002461"
     CDS             complement(1379..1984)
                     /locus_tag="ACG8M6_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0458080.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="ACG8M6_002462"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="ACG8M6_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2881
                     /locus_tag="ACG8M6_002463"
                     /pseudo
     CDS             2666..2881
                     /locus_tag="ACG8M6_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3019..3990
                     /locus_tag="ACG8M6_002464"
     CDS             3019..3990
                     /locus_tag="ACG8M6_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0458081.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="ACG8M6_002465"
     CDS             4130..5443
                     /locus_tag="ACG8M6_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0458082.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="ACG8M6_002466"
     CDS             5486..6352
                     /locus_tag="ACG8M6_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0458083.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="ACG8M6_002467"
     CDS             6349..7197
                     /locus_tag="ACG8M6_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0458084.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="ACG8M6_002468"
     CDS             7211..8683
                     /locus_tag="ACG8M6_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0458085.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="ACG8M6_002469"
     CDS             8700..>8942
                     /locus_tag="ACG8M6_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0458086.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2831 a         1515 c         1830 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtattttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DAZKOB010000110         8819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000110 DAZKOB010000000
VERSION     DAZKOB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.112"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1094
                     /locus_tag="ACG8M6_002470"
     CDS             33..1094
                     /locus_tag="ACG8M6_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0458087.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1097..1993
                     /gene="murQ"
                     /locus_tag="ACG8M6_002471"
     CDS             1097..1993
                     /gene="murQ"
                     /locus_tag="ACG8M6_002471"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0458088.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2007..3989
                     /locus_tag="ACG8M6_002472"
     CDS             2007..3989
                     /locus_tag="ACG8M6_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0458089.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACG8M6_002473"
     CDS             complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACG8M6_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0458090.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4711..6135
                     /locus_tag="ACG8M6_002474"
     CDS             4711..6135
                     /locus_tag="ACG8M6_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0458091.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6316..7089)
                     /locus_tag="ACG8M6_002475"
     CDS             complement(6316..7089)
                     /locus_tag="ACG8M6_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0458092.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7086..7991)
                     /locus_tag="ACG8M6_002476"
     CDS             complement(7086..7991)
                     /locus_tag="ACG8M6_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0458093.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8145..8465)
                     /locus_tag="ACG8M6_002477"
     CDS             complement(8145..8465)
                     /locus_tag="ACG8M6_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0458094.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2774 a         1583 c         1876 g         2586 t
ORIGIN      
        1 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
       61 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
      121 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
      181 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
      241 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
      301 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
      361 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
      421 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
      481 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
      541 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
      601 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
      661 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
      721 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
      781 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
      841 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
      901 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
      961 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     1021 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     1081 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     1141 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     1201 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     1261 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     1321 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     1381 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     1441 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     1501 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     1561 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     1621 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     1681 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     1741 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     1801 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     1861 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     1921 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     1981 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     2041 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     2101 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     2161 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     2221 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     2281 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     2341 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     2401 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     2461 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
     2521 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
     2581 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
     2641 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
     2701 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
     2761 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
     2821 tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct
     2881 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
     2941 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
     3001 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
     3061 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
     3121 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
     3181 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
     3241 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
     3301 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
     3361 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
     3421 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
     3481 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
     3541 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
     3601 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
     3661 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
     3721 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
     3781 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
     3841 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
     3901 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
     3961 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
     4021 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
     4081 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
     4141 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
     4201 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
     4261 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
     4321 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
     4381 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
     4441 tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat
     4501 ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt
     4561 aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg tagaattata
     4621 tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat actttttgat
     4681 aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa aaaaggaatt
     4741 ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg cgtatttggt
     4801 attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg ggtactcgta
     4861 ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa acgtccagat
     4921 ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc tggttttatc
     4981 agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc aacattgttg
     5041 atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc
     5101 gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg agtcgaaaat
     5161 gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt tttatttatt
     5221 gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc tagcgacatt
     5281 atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag ttgcatcatg
     5341 gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa
     5401 aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat tattatttac
     5461 gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca aacgatcaag
     5521 gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg
     5581 atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac gatcttaccg
     5641 attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg gggaaaactc
     5701 aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac gaatgtcttt
     5761 ttactttcat tattattcac tgacagtgct tataactttg cttacacact tggtacagca
     5821 gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg
     5881 aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt atgggctatg
     5941 ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc aggcatttat
     6001 ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca cttagataag
     6061 attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt tgccactggg
     6121 atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc tgtcgtttat
     6181 tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc tttttcagaa
     6241 taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt tcacagcctc
     6301 ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta atgctaagaa
     6361 caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat gagtcaattg
     6421 cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga tcggattcca
     6481 tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga ttgacgaagc
     6541 aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa agcaagcaat
     6601 catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag tataagtgaa
     6661 caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa
     6721 aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat aaacagaata
     6781 aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact ttcgataaag
     6841 gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg cagctgcaat
     6901 catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt caactggatc
     6961 aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa caactaatag
     7021 aacagataaa acagcagttg actttttctt gatcaattta aataattctt gttttacaag
     7081 caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag agaagattcc
     7141 aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga caatagctgg
     7201 atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc aggatcttcc
     7261 aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt tgtagagata
     7321 acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt gatgatcttt
     7381 ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag taagtgactg
     7441 gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg cacatctctt
     7501 gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga cggatggttc
     7561 agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga tttcgctttt
     7621 ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc agatacatcg
     7681 ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata gatacccggg
     7741 tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga aatctcattt
     7801 tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc tttcatgatc
     7861 gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg ggataagcta
     7921 aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc ttggaccgaa
     7981 agacttttca aaaatattca ctccttttct atagatagta caagcataca cctgatgagt
     8041 ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa ttgaatatta
     8101 aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg gccgctagta
     8161 atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt ggaaagattc
     8221 agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc aatccctgtc
     8281 cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa aaagtaaaaa
     8341 ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa accatcactc
     8401 catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa ttccatccat
     8461 gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa aatcatccct
     8521 tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat tgtatatctt
     8581 tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt agtaaagtgt
     8641 accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg caatttcata
     8701 gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct tatttctata
     8761 tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat aatttttgt
//
LOCUS       DAZKOB010000111         8721 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000111 DAZKOB010000000
VERSION     DAZKOB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.113"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(318..2699)
                     /locus_tag="ACG8M6_002478"
     CDS             complement(318..2699)
                     /locus_tag="ACG8M6_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0458095.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2689..3336)
                     /gene="recU"
                     /locus_tag="ACG8M6_002479"
     CDS             complement(2689..3336)
                     /gene="recU"
                     /locus_tag="ACG8M6_002479"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0458096.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3400..3939
                     /locus_tag="ACG8M6_002480"
     CDS             3400..3939
                     /locus_tag="ACG8M6_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0458097.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4029..4448
                     /gene="gpsB"
                     /locus_tag="ACG8M6_002481"
     CDS             4029..4448
                     /gene="gpsB"
                     /locus_tag="ACG8M6_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0458098.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4470..4837
                     /gene="rnpB"
                     /locus_tag="ACG8M6_002482"
     ncRNA           4470..4837
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8M6_002482"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5046..6212
                     /locus_tag="ACG8M6_002483"
     CDS             5046..6212
                     /locus_tag="ACG8M6_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0458099.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6258..7754
                     /locus_tag="ACG8M6_002484"
     CDS             6258..7754
                     /locus_tag="ACG8M6_002484"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0458100.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7835..8518
                     /locus_tag="ACG8M6_002485"
     CDS             7835..8518
                     /locus_tag="ACG8M6_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0458101.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8527..>8721
                     /locus_tag="ACG8M6_002486"
     CDS             8527..>8721
                     /locus_tag="ACG8M6_002486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458102.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRLF"
BASE COUNT         2749 a         1577 c         1845 g         2550 t
ORIGIN      
        1 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
       61 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
      121 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
      181 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
      241 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
      301 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
      361 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
      421 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
      481 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
      541 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
      601 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
      661 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
      721 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
      781 gattctgaag tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa
      841 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
      901 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
      961 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     1021 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     1081 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     1141 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     1201 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     1261 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     1321 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     1381 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     1441 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     1501 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     1561 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     1621 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     1681 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     1741 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     1801 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     1861 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     1921 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     1981 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     2041 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     2101 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     2161 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     2221 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     2281 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     2341 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     2401 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     2461 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     2521 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     2581 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     2641 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     2701 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     2761 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     2821 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     2881 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     2941 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     3001 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     3061 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     3121 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     3181 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     3241 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     3301 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     3361 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     3421 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     3481 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     3541 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     3601 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     3661 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     3721 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     3781 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     3841 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     3901 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     3961 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     4021 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     4081 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     4141 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     4201 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     4261 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     4321 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     4381 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     4441 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     4501 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     4561 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     4621 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     4681 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     4741 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     4801 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     4861 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     4921 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     4981 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     5041 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     5101 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     5161 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     5221 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     5281 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     5341 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     5401 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     5461 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     5521 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     5581 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     5641 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     5701 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     5761 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     5821 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     5881 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     5941 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
     6001 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
     6061 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
     6121 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
     6181 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
     6241 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
     6301 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
     6361 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
     6421 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
     6481 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
     6541 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
     6601 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
     6661 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
     6721 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
     6781 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
     6841 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
     6901 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
     6961 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
     7021 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
     7081 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
     7141 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
     7201 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
     7261 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
     7321 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
     7381 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
     7441 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
     7501 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
     7561 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
     7621 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
     7681 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
     7741 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
     7801 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
     7861 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
     7921 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
     7981 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
     8041 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
     8101 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
     8161 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
     8221 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
     8281 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
     8341 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
     8401 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
     8461 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
     8521 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
     8581 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
     8641 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
     8701 gcaggagaac accgtttatt c
//
LOCUS       DAZKOB010000112         8494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000112 DAZKOB010000000
VERSION     DAZKOB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.114"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG8M6_002487"
     CDS             complement(<1..149)
                     /locus_tag="ACG8M6_002487"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0458103.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG8M6_002488"
     CDS             complement(593..1714)
                     /locus_tag="ACG8M6_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0458104.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG8M6_002489"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG8M6_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0458105.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG8M6_002490"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG8M6_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0458106.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG8M6_002491"
     CDS             complement(4304..5215)
                     /locus_tag="ACG8M6_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0458107.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG8M6_002492"
     CDS             complement(5232..6239)
                     /locus_tag="ACG8M6_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0458108.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG8M6_002493"
     CDS             complement(6236..8365)
                     /locus_tag="ACG8M6_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0458109.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG8M6_002494"
     CDS             complement(8370..8480)
                     /locus_tag="ACG8M6_002494"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458110.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZKOB010000113         8312 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000113 DAZKOB010000000
VERSION     DAZKOB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.115"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8M6_002495"
     CDS             476..1339
                     /locus_tag="ACG8M6_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0458111.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8M6_002496"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8M6_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0458112.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8M6_002497"
     CDS             3339..3860
                     /locus_tag="ACG8M6_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0458113.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8M6_002498"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8M6_002498"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0458114.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8M6_002499"
     CDS             5941..6759
                     /locus_tag="ACG8M6_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0458115.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8M6_002500"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8M6_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458116.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8M6_002501"
     CDS             7228..7806
                     /locus_tag="ACG8M6_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0458117.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8M6_002502"
     CDS             7901..8038
                     /locus_tag="ACG8M6_002502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458118.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2503 a         1489 c         1640 g         2680 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gt
//
LOCUS       DAZKOB010000114         8071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000114 DAZKOB010000000
VERSION     DAZKOB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.116"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG8M6_002503"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG8M6_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG8M6_002504"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG8M6_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG8M6_002505"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG8M6_002505"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0458119.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8M6_002506"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG8M6_002506"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0458120.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG8M6_002507"
     CDS             5704..6489
                     /locus_tag="ACG8M6_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0458121.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG8M6_002508"
     CDS             6502..7353
                     /locus_tag="ACG8M6_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0458122.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG8M6_002509"
     CDS             complement(7477..7929)
                     /locus_tag="ACG8M6_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458123.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         2714 a         1373 c         1711 g         2273 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg t
//
LOCUS       DAZKOB010000115         7933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000115 DAZKOB010000000
VERSION     DAZKOB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.117"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1130
                     /locus_tag="ACG8M6_002510"
     CDS             150..1130
                     /locus_tag="ACG8M6_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0458124.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            1252..2670
                     /locus_tag="ACG8M6_002511"
     CDS             1252..2670
                     /locus_tag="ACG8M6_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0458125.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            2741..4210
                     /locus_tag="ACG8M6_002512"
     CDS             2741..4210
                     /locus_tag="ACG8M6_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0458126.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            4220..4687
                     /locus_tag="ACG8M6_002513"
     CDS             4220..4687
                     /locus_tag="ACG8M6_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0458127.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            4723..5595
                     /gene="scrK"
                     /locus_tag="ACG8M6_002514"
     CDS             4723..5595
                     /gene="scrK"
                     /locus_tag="ACG8M6_002514"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0458128.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            5677..>7933
                     /locus_tag="ACG8M6_002515"
     CDS             5677..>7933
                     /locus_tag="ACG8M6_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0458129.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNE"
BASE COUNT         2749 a         1083 c         1591 g         2510 t
ORIGIN      
        1 tatatcttta aacagctatt gccagataaa aataaatgat attatatata taaatactta
       61 tccgaaaagg ggaatgagag agtcaaggtt cttggaatgt cgaatagtcg agtaggtaga
      121 ttttctatct acaaccttgt tgtttaaaaa tgaaaaaaat tggaattaga gaagttgcga
      181 aagaggcaaa tgtttctcct acgactgtgt cgagggtatt gaatgatcgt ggatatttga
      241 gtgaggaaac aaaaagaaaa gtttttgatg ccatgaaaaa attagaatac tatcctaatg
      301 aactggcgag agcacttttt aaaaacgaaa catataccgt aggtttgatt tttcctacaa
      361 ttactaatcc atttcacgct gagttaattc aagatatcga gttcgagctt tccagcaggg
      421 gatataaagt gttgctgtgc aatagcttaa acaatccaac aaaagaaaaa gagtacttga
      481 cgatgttgag aaagaatcag gttgatggaa ttatagtagg aacgcacaat aaagatattg
      541 cggattatga tattccaggg ctacctattg tagcgattga tagaagtctt ggtgaaaata
      601 cagttacagt atcttgtgat aattacaggg gtggggaatt agctactcaa ctacttgtaa
      661 atcatggctg tcagaatata ctgtgtattc ggggagatag taagataaag ctacccgcga
      721 atagtaggac tttagcatat aaagatgttt tgaataaatt catgctggaa ccgttagtcc
      781 tagaagtacc atttgtaaaa gaaatcaatg aaaaaaatga aataattaag acatatctgg
      841 aaactcatcc aaatattgac ggggtttttg caggagatga tttattggct tctttggcga
      901 ttaacaactt aggaagttcg ggcaaaagag ttccaaaaga ggttaaagtg attggatttg
      961 atggagctca acaaacattg atttataatc cagaattatc aactattcaa cagcctatag
     1021 atcaaatatc taaagtagca gtaaaaaaat tattgaatat gattaagggc gaaaaggaga
     1081 cggatgaact ttcacttcca gtaaagctca taaaaaaaaa tacagcataa gttaaatcgg
     1141 cgaaaaattc cttcgtcgat ttaaaaatat ttttatgaaa cgggttgaca tatgaaacgg
     1201 gttgacatat attagttgtg aagataaaaa ccttttcact ggaggaagaa aatggagaaa
     1261 ttattaatta aaagcaataa tcatcaactt tactaccaac ctaaggatgt ttgggtcgga
     1321 gatgttatgc cttacgggaa agaaggaaaa ttctttattt atcatcaacg tgatactaga
     1381 aatccagttc cttttggaga accgtttggc tgggctttag caactacgaa agattttgtt
     1441 cattttgaag actttgggga atctctttta agaggggatg acaatgagag agaccagttt
     1501 atctacgctg gttctgtatt tgaggcaaat ggaaaggttc acgcatttta tacagggtat
     1561 aatcgtgaat tcttagcgca gggaaagaca tctcaagtgt tgcttcatgc tgagagtgat
     1621 gattttgttc attgggaaaa atcagaaaca gcattggagt tagaaccaca agaagggtat
     1681 gatattcgaa actggagaga tccatttgtc gtttgggatg atgaaaaaga agaatatttg
     1741 ctaattttag gggcgcgaaa gggtcaggat aaacatctac aaaccggacg tttagtggaa
     1801 tttacatcaa aagatttaac taattgggaa ttcaaaggtg atttttgggc tccgggattg
     1861 tataccatgt ttgaaatgcc agaacttttc aagattggtg actggtggta tcttgtctat
     1921 tcagaatact cagatggaaa taaaatactc tatcgaatga gtcaaagttt ggaaggtcct
     1981 tggattaagc ctaaagatga agcttttgat ggacgggctt attatgcagg tagaacagct
     2041 tatgatggaa aacgtcgagt cctatttgga tgggtaccaa caaaagatca agaaaatgat
     2101 aaaaacaatt atttatgggg aggtactttg gttcctcatg aagtctatca aaaagaggat
     2161 ttttcacttg gggtaaaacc catagacgaa gtgtggaatg cattcataaa tttacatgat
     2221 attgccgatg tagagataga aacagcagac acaagagaag aagtaacgct tgtcaaaaat
     2281 agtggaactt tgtttaattt agaaacaaca atttcatttg atgagaatct tagtgacttt
     2341 tcactgagat tatataaaaa ccttgagaca gatgaatctt acgaatatcg cttcaattta
     2401 gatgaggggc gtcttacatt tgataaaaac ccttgttacc cttggtttca atatatggat
     2461 aaaggactta ttagacctat atctttaaag cctaaccaat cttataaact aaaagtgatt
     2521 gttgatgaca atataatgac actttatatt gatggaatcg ctcttaacgc tcgtgtacat
     2581 ggttgttttg gtcaagcaat tactattaca gcaacaaatg gatcaatgac tgcaataaat
     2641 accaagtttt caaatgaaat aaagaaataa taaacgtcaa tcatgtggca ggtctgaaaa
     2701 tcaaacatga cctgtcacat ttaaaaggga ggataaatga atggactaca aaaaaattgc
     2761 cgaagatgta ttggaaaacg ttggaggaaa agagaatatc tcaagcgcta cacactgcat
     2821 tactcgttta cgtttaattc ttaaagacaa aagcaaagcg aatatggaaa tgcttgagga
     2881 aatagaaggt gcaaaaggtg ttcttttcaa tagtggacag ttacaagtaa ttttcggaac
     2941 aggtataatt gaaaaggttt acaaagagtt cgttgaattg acaggagcta aggaagtatc
     3001 cgtttctgaa atgaaagacg aaggagtcaa taattcagga aatagacttc aacgagcttt
     3061 taaagttttc tcggatatct ttattccgat cattccagct tttattgggg cagcaatggt
     3121 tttaggaatt cgttcgttga ttaccactcc tggaatgttt ggaatggatg gctcattcgc
     3181 tgatcacaat gtattcctaa atgatttatc caaatttctg ggtattattg caaccacatt
     3241 tgattattta ccagttttag ttatgtattc tgcaacaaaa cgttttggcg gaaaccctat
     3301 tttaggatta cttgtaggat ttgttatgat tcatccagga ctagccaatc ggaatgattt
     3361 cgtcatggga actataaagc ctgattattg gcatttattt ggattaagca ttccagcagt
     3421 tgcgtttcaa gggggtgttt ttccagcaat tttgacttct tggtttttag caaaagttga
     3481 gaaaattaca aataaatatt ctcctcaagt acttgcttac attttagtcc caactgtgac
     3541 aattgttctt gccaactttg cactattttt agtgtttggt ccattgggga attttatcgg
     3601 taatgtttta ggtggaatca ttgatttcct atatatgaag atgggtgcct ttggtgcttt
     3661 tgtttttgca ggcgcgttac aaccattagt tgtaacagga acacaacatg ctattcaagg
     3721 tattgaggca aacttagtcg caacaactgg attcaattat atccaaccta tttggtcagt
     3781 ttcaattatt gcccaaggtg gcggttgtat tggtatgtat ttactcttta agaaaaaatc
     3841 taaagataga gatattgcga tttcaagctt tattccaaca ctggtaggta ttactgaacc
     3901 ggctatcttt gctgttaatt tgaaatattc aatcgttcct tttgtttgct cgtttattgg
     3961 tgctggtttt ggtggagcat ttatgaaaat ttttgatgta aaagccattg gacaaggatt
     4021 gactgtattg ccaggtctga ctattgctaa tccagttctt ccttatatat ttggtaatct
     4081 catagcattc attttaccaa tcatttttat tctcgcttat gataaatata aagtgtttat
     4141 ccctgaagct aaacaccgta aatttgcaga aaaaactgac tcaaaaaaat tgccagttca
     4201 agtaaagtga gatgagagca tgttaaattt ttttagaaaa aatgaaataa gcgttgtagc
     4261 acctgcaacc ggtgtttttg aaaaactgga gaaattaagt gatccagtct tttcaaaagg
     4321 aatgatggga caaggatttg caattgatgc aaaaaacgaa acgattgtat ctccaatcga
     4381 tggttttgtt tcaagtattt ttcctacaaa acatgcctta ggaataaaaa caaaatcagg
     4441 aattgagata cttgttcatg taggaataga tacagttgag ctaaatggtg agggatttga
     4501 tattaaagtt caagaaggac aaaaagttaa ggctggggat attcttatga ccattaattt
     4561 tgtagttttt gaaaagaatc acaagactaa agatgtcatt gttgtttttc cagactacca
     4621 agacgaaatt ttcattccga aacttgaaga agtaaaacaa ggacaagaat tgtttgttct
     4681 tggctaatga taagtaatta ataatagaat cgaggaaaat gtatggtttt tggggctatt
     4741 gaagctggtg gaacaaaatt tgtttgtgcc attggagata aagatttgaa catatttgaa
     4801 aaagtcagct ttcccacgac tacacccaaa gaaacaatga atttagtttt agattttttt
     4861 aatcatcata aagatgattt agaaggtatc ggagtgggat cgttcggtcc tattgatatt
     4921 cattgtgaat ctaaaacgta tggcttcatt acatcaacgc caaaacttgc ttggcaggat
     4981 tttgatttta ttggaagttt aaaaaagcaa cttaatattc caactgcttg gacgactgat
     5041 gtaaatgcct cttgctatgg tgaatatgtt tctggaaatg gcagaggcct ttcaagtgtt
     5101 gtttactata ccgtaggaac gggcatagga ggtggcgcta tccaaaacgg aaacttcatt
     5161 gaaggtttta gtcatccaga aatgggacat atgcttgtaa aacgacacaa taaagacaac
     5221 ttttctggga catgtccatt ccacggagac tgtttagagg gcttagctgc tggtccggca
     5281 atcgaaagaa gatcagggaa aaagggacaa gaagtgactg aaaatgacgt tacttgggaa
     5341 atagaagctt attacattgc tcagtgtgca tataatacaa cattgatgtt atcacctgat
     5401 gtcattatat ttggtggcgg agtaatgaaa caaaaacact taaaaaagaa agtacaagat
     5461 aagtttattg agttgatcaa cggatatgtc gctattcccg atgttgacaa ttatattatg
     5521 acaccaaaat tgttagataa ttctggtatt gtaggttgtt tagctttagc aagagacgtt
     5581 aaagaaaaat cttaatttgg aaaaaagatc gtctcgacta tttgtttggt tgatgcgatc
     5641 ttttttttca attagtaatt gaagggacgc ctaaaagtga aaaaaaaaat gaaagatgaa
     5701 gtattagagt atatcaaaaa taatttaaag tattacgatt tttctgctca agatattgca
     5761 atgaaatttt gtattaaaag aaatgtcgct tcccactatt tgaatcagtt attttcagat
     5821 ggaaaattac taaagaatga ttctgttaga ccagtaatgt tcaagtataa tcaacaaaag
     5881 ccaaaagact gcttttctaa gtttattgga gcagacatat ctttgaaatc cactatagat
     5941 aaatgcaaag ctactgtaat gtatccccct aatggcctcc cattaataat aaaaggaaac
     6001 agtggcgttg gaaaaagttt tttagcaagt ttgatctatc agtatgctct tgatagaaaa
     6061 gtaattcata atgacgcaaa atttgttgtt gtgaattgtg cagattatgc aaataatcca
     6121 gagctattgt cagcagtttt atttggatat aaaaaaggag cctttactgg ggctgaaaaa
     6181 gatactgcag ggatgctatc ttctgctaat ggaggatacc tttttttaga tgaggttcac
     6241 aatctaagtg cagaaaacca agagaaattg tttcttctta tggattctca aaagtatcgt
     6301 aaacttggtg aaagtgtgca atgggagtat gcaaatgttc gccttatctt agccacaaca
     6361 gaagataaaa atacctcttt gttagcaact tttagaagac gcatcccagc tgaaatcact
     6421 ctgccagatt acaataatag aagtacatct gaaaaaattc aattattatt tgaatttctt
     6481 aaagatgaag caattacaat tgattcaaaa atattttgtt cgattgaact ttttactgat
     6541 cttttatcga aaacatttga aggaaatgtt ggagaattga ggaatgaaat caagcttctt
     6601 tgtgcagaag gttatttagg aaataagaga aagtcttcaa tttatttagg ggacaaacta
     6661 gacagcggtt tttggataga tccagaaatt agtatagatt taaaaaaaat gtttcttaaa
     6721 tcactttctg aaattgattt gattgaactt tttcaaaatc acatttcaat tgaagaaagc
     6781 atgagtcaat taagagatag aatacttaaa gagtgtgcag gttcttctga gtataattat
     6841 ttagaaagtg acgaatttat agcattaaga aactatgttg ttaataaaat atcaccaatt
     6901 attgattcga caggtctatg tttattagat gaattcttgg cggatatcag tctatttata
     6961 atgtttattg atgtaattga agaaacatca agaaattttt tgctgtctaa gttcagaaaa
     7021 tttcgtatga aaaatgataa acaaaaatta ttggcaaatg agatttggag tagtttagaa
     7081 gtcgaagaaa gtaaacaaga acttttgttg aaatggatac tttttcttgt aaagaagttc
     7141 tatgtgaaaa taccagaaac ccattgtctg atagttatgc acggtaaaat aacagcttct
     7201 gcgatcgcaa gggaaacaaa taagttgctg aatacatatg tttatgaggc tttcgatatg
     7261 ccaattgaag gtgagactag tgacttaatt aacctaatca acaatttttg taaaagtata
     7321 gatacgacga atggactcat tctgctagtt gatatgggat cattggaaca aatgtatgaa
     7381 aaaattgaat ccaatgtaat aggtgatttg gtaattttaa acaatgtttc tacagcttta
     7441 gcgattgaat gtgggattca agtttgccaa aaaaagccaa ttagtcattt ttaccagatg
     7501 gattttgatt cgtttcaagt taaagttcag tattataaag gattatcaca aaagaaaaat
     7561 gtaatagtat cttgtctctc tggtgagggg atttctgaaa aagttaagga tattttaaaa
     7621 cggtatctcg ataatcaagt ggagattttg atttttgatt ttaacgcatt aaagaaaata
     7681 gcaaaggaaa aagatactat gatttttaag aatacaattt gtgttctttc aaccactgaa
     7741 ttttacattc aaggaatcga ttgtttaaat ttagaaaatc taattaatgg gaatcaaacc
     7801 ttagaaaaat taaataagta tatggactcg gatagctgtg agttctgctt aaatgaatta
     7861 gttaaactct ttactattga aggcgctagt actaagcttc gctttttaga ttccaaaaaa
     7921 gtattcaatg aga
//
LOCUS       DAZKOB010000116         7851 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000116 DAZKOB010000000
VERSION     DAZKOB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.118"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..745
                     /locus_tag="ACG8M6_002516"
     CDS             44..745
                     /locus_tag="ACG8M6_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0458130.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1354..2370)
                     /gene="tsaD"
                     /locus_tag="ACG8M6_002517"
     CDS             complement(1354..2370)
                     /gene="tsaD"
                     /locus_tag="ACG8M6_002517"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0458131.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2367..2831)
                     /gene="rimI"
                     /locus_tag="ACG8M6_002518"
     CDS             complement(2367..2831)
                     /gene="rimI"
                     /locus_tag="ACG8M6_002518"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0458132.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2838..3380)
                     /gene="rimI"
                     /locus_tag="ACG8M6_002519"
     CDS             complement(2838..3380)
                     /gene="rimI"
                     /locus_tag="ACG8M6_002519"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0458133.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3364..4086)
                     /gene="tsaB"
                     /locus_tag="ACG8M6_002520"
     CDS             complement(3364..4086)
                     /gene="tsaB"
                     /locus_tag="ACG8M6_002520"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0458134.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4277..5257)
                     /locus_tag="ACG8M6_002521"
     CDS             complement(4277..5257)
                     /locus_tag="ACG8M6_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0458135.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5281..6765)
                     /gene="galT"
                     /locus_tag="ACG8M6_002522"
     CDS             complement(5281..6765)
                     /gene="galT"
                     /locus_tag="ACG8M6_002522"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0458136.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6777..7766)
                     /gene="galE"
                     /locus_tag="ACG8M6_002523"
     CDS             complement(6777..7766)
                     /gene="galE"
                     /locus_tag="ACG8M6_002523"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0458137.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2197 a         1694 c         1395 g         2565 t
ORIGIN      
        1 aagtgacaat aaatatagtg aacaataagg agaggacttt ttaatgcgtg ttaataaaat
       61 cgattttcac ttttcaaaac tcagatatca acctgatttt gaacatttct cggatgaaga
      121 gttccaacag atcaaggaac atactgttct tcgttcttat aaaaaaggac agatcctttt
      181 cgacgatggt gatcccagac aacggttgta tatcttgacc aaaggactcg ttcgtttaga
      241 acgttatgat gaaagtgcaa cttattacta ctatgactat gtgaatacaa ataacctttt
      301 tcctatgtca ggaatgtttg gagaagagac ctacacttat acagctcaag cgatgactga
      361 tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa ttagcacgaa aaaatcccga
      421 acagttgatc tatatgatac aaaaaatttc taaagtagtt gaaatacatg aattgcgtat
      481 ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa caaaatttat tcattttgaa
      541 agaagaattg ggcgtaccaa caaaagatgg tcatatcacc attccttatc cgatcacctt
      601 aaaagaatta gcagtgaata gcggcacgac acgtgaaact gctggacaag taatcaaaca
      661 actacgtgat tccgggctgt tagattatga caaaaagcgt tttactttct gtaagacagc
      721 tactactcca gctatgagag aataacgcga acaaaaataa gcagtgaccc tgataaatat
      781 cagggtcact gcttattttc ctccctaaaa cttctgaaag atccggcagt tttagatcac
      841 tggacaagac tcaaagattt tctatgtgtg tttcagttat gtaatattca ctgtttgcaa
      901 aaaatactgt tagattaact agatgaagcg tcttgtcctc atccgatcta gattattcga
      961 tattcctcga caataaacga ttactcagtg agggcaatca gaggaacgga taatgataaa
     1021 taagctgtaa aaaatagcaa aataacaata atgattaaaa gtatgtaaaa aatagggctt
     1081 tgacaaaaac gtgtcacagc cctatttcga ttaaacggtg tgtacctgtc aactcttcgg
     1141 tcacaagtca taatgttcat aaacatgagg agctgttttt tgaaattgtt ccttgtgaat
     1201 cagagttaaa cgacaggctt acgaactctt gattaaacgg cgttcaaaag cttgcatacg
     1261 cggtaataag aacagccagg tcaaaaacat gagaagccgt ttttcctggg ctgttcttat
     1321 tacttgatgc agggcagctt tttcacaacc tctttattca gataaaacca agcttggatc
     1381 agcattcaac tgatagctag caaaatttct tttttgcatt tccacatgtg ctgctgctcc
     1441 aatcattgca gcattatctc cacaaagtcg aagaggggga atcactagtt ctacctcagg
     1501 gagtttagtt gccaaagcat cctgcaatcc ttcacgcaac ccttgattgg ccgctacacc
     1561 tccagctata atcagttgct tgactggata attttgacaa gctcgaagtg ttttagtaat
     1621 caatacatcg atcacacttg cttgaaagct agctgctaaa tcatttttgt ctagatcttc
     1681 tccacgctgt tgtgcattat gcaccaaatt gataaatgca ctttttaatc cgctaaaact
     1741 gaaatcataa ttatcttcat ggatcatcgc tcgcggaaaa tggtaattat cttttccctg
     1801 atgagctagc tgatcgattt cttttccgct tgggtaagaa agacctaata ctcttccaac
     1861 tttgtcataa gcttctcctg ctgcgtcatc tcttgtctcc ccaatgattt catatgatcc
     1921 atcttcttgc atatagacta attctgtatg tcctccgcta acaagaagtg ccatcaatgg
     1981 aaactggaat ggtttaacca gacgggctgc atagatatgc cctgccatat ggtttacagg
     2041 aataagcggc aattggtgtg cccaagcaaa agccttagct gcagaaatac caatcagcaa
     2101 cgagccgaca agtcctggtc cataagtgac agcgactgcc gataaatctt ctgccgacac
     2161 accagcttct accagtgcat cttccaagca taaggtgatt tgttctacgt gatggcggct
     2221 agctacttca ggtactacgc caccaaaacg tttatgactg tttatttgag aagcaacgat
     2281 gttagataag atctctgttc catttctaac gaccgctaca ctcgtttcat cacaacttga
     2341 ttcaatcgcc agaatcaatt cttcattcat ttcatcgtct ccgtttttaa ctttctgctc
     2401 ataatgatcg catcttccgt cggattgcga taatatgatt tccgtttacc taatatacga
     2461 aacttttctg attcataaag ttttcttgct gcttcattag atagacgtac ttctaaaaaa
     2521 acatgcagcg aatcttttat ttgttccgta atcagtaatt gtaacaaatg ccgtgcatgc
     2581 ccttttcctt gttctgaaac tgcaacagca atatttgtga tctcgacttc atctattact
     2641 ttcgagtatc caataaagcc acagatctgg ttatcttcag ctaaaagcag ataatcagag
     2701 tgaggctgtt ggatgtctgc caaaaactgc attttcgtcc aaggtgaacc atgtggataa
     2761 gcattttcgc taatcatcca tacgcattct gccaagtacg cttcatcaaa atcttttttt
     2821 ctgcaaatca actaatctca ctcattttct aaaagtttta tcatatccaa cgcatcttct
     2881 tttgtttcag tgtaatatct ttttttgatc gttgttgctt gaaaccccaa tttccgataa
     2941 agtctttgtg catcttgatt actcatacgc acttctaatg acatcgtctc acagcgattc
     3001 attatagcaa agttctcgat ttcgtcaata agcagcgaac cgattcgttt acgctgatat
     3061 gccggtcgaa cggcaatatt cgtgatatgg caatctaaac cgaacgctct tgaaccgata
     3121 aaaccaatca ttcttccatt atcaaagata cctagatata gatgaggaag cggtgacttc
     3181 aattctgata taaaggcact ttttgtccat ggcaattcgc cattatagac ttctcgttcg
     3241 acatccagca gctctttgat atcttctgta gtcaattctc ttagtacgta acgacgatcg
     3301 atttgttcga ctgttttttc tgtataaatc attttatctt gaaaaaagct tctggcatac
     3361 gctctaaatt ttttcaacat aactttcgtc tccaggtgtg tgcgtctcca accatttttc
     3421 ttctgcttct actcgtttta aatagcgagg aagaaaagct tgtttctctg caactggttc
     3481 aactaaagca cctaattgag ccacagttac accacttgga atatcccatg ctggaactgt
     3541 attaatcatc gcactaggca atatttcctt tatttcagag aagaatttgt gacaatcact
     3601 cccgacgaaa taaatagctt cattcagctt ttgtagttgt gtgaacaaat cagaagcaga
     3661 aatatgtaca tcttcaagta cagtttctaa tctattttct ttccagcggt atgccccagc
     3721 gtatacattt tttcttcgag catcaaacat gggcacaata atattatcca caccgatgca
     3781 attagccgcg atggttttca gactagatac tcctactagc tctttatcta atgtataagc
     3841 aatcgttttt gctgtcgtta cacctatacg aagccctgta taagaaccgg gaccatcaga
     3901 aaccgcgatt cggtcaatgt cttttggact tatttgaaga tctgcaaaca attggtcgat
     3961 tgcaggcatc agtgttacac tgtggttgcg tttgatattt gtttgtattt gtccaagaat
     4021 ttgttcgtct tctaccacac cgatcgccaa agtttggttt gcagtatcta ttcctaatgt
     4081 aatcatatat ttgatccttt ctgctatgac attctcgcct tccattgtag catacttttt
     4141 tattctgagg atgaaaatac caaaggcaaa actagtacaa taatacatga ttatctgctg
     4201 gcgggcaaat tttgcccctt cctgaaacac aaaaaagctg cggtatcact ccattttccc
     4261 acagcttttc catcttttat ttcgttgatt ctcgaataat caactctgtt tccacaataa
     4321 tctttcttga aaccggtgct ttggaattac ttaattgaag aatcgtctcc actgctagct
     4381 ctcccatcca ttcggtataa acttttacag tagaaagcga gggcgttaca tatttcgcta
     4441 cactgacatc attgaagccc atcacagaaa tatcttctgg taccactaaa cctttttctt
     4501 gaatcgcttt cagtgcgcca atcgctaatg catcattagc tgcaaaaagc gcatcaggaa
     4561 catgcggatt cgtttttaaa tactgcttca ctgcttgata tcctgactcg acagaaaaat
     4621 ctgcttcgat aattggacct tcgtcgtagc ctttttctgc caaaacttgt tgaaatgcca
     4681 taattctggg atcttttagc cgctgatggt ttttcttcgt atattctgtt ccagcaagca
     4741 tagcgatatg ttgcgcttta tgggagacaa agtgttcaat gactttttga acacttgact
     4801 taaagtcgac aactagagag tcgattcctt gagaagtgcc gtcagaatct accaaaagca
     4861 aggcattttg ggatcctttc atttctaata actgagcttc gtcgaatttt cctagtgcga
     4921 tcgttccatc tgccggaaga tcactaatgg aatcccacgt ttctttcaat aaagtaacgt
     4981 ttgcttcttc tgcctttttt tcgattccta ggcgaataga taaataataa agatcttcaa
     5041 gctcttccgt atcgtcgtac cactggatca ggcgaagcgt ttgcttagta tttgctgcat
     5101 ttttacgatg tttcgtataa tttaagcgtt ctgctgcttc aaaaattttt tgctttgtgg
     5161 ctaagccaac ggaaagatca ggatcatagt tgagaactct tgaaacagta gcaggagaaa
     5221 ctcctgccaa ctgcgcaatg tctttaattg ttgccatttg tctctcctcc ttttttacgt
     5281 ttataaaatc gcagtaaatc gttcaaacgc tgcctgtcct ttttcatctc gcttgaatac
     5341 tccagcgtct tccagtacac gtgcaaaaac ctgacctaca gatttttgta cgacatcggt
     5401 gaccgtatct ttcgtcaaat caggatgttc tttttttaat tgttctgccc acggacgatg
     5461 atattcagcc atctggtttt cttctcctaa taagaaatgc tctacttcta ctagttctgg
     5521 tttcaaacga ggtggtaaaa cagcaagccc cattacttcg atcaacccaa tgttctcctt
     5581 tttgatatgt tggacatctt tatgtggatg aaaaatccca tcaggatgtt cttctgaaac
     5641 attgttgtct cgaaggacaa ggtccaactc aaagtaattg tcccttcttc gtgcaattgg
     5701 cgtaatcgta tggtgtggtg taccatctgc tgaaaaagct ctgatagata ctgtttcatc
     5761 cgaatatgct ttccattttt ccaatactga gttagctgct tcaataagca agtttttctc
     5821 tgttgattgc aaacggatca cagacatcgg ccatttcacg actcctgcga tgacttgcgg
     5881 ataatcttct agtttgataa gtttttctat cggtgctttt gccataggaa attcatgacg
     5941 tcctgcctga taatgatcat gagaaagaat cgatcctcca acaattggta agtcagcatt
     6001 cgaaccaaca aagtaatgag gcaagacttc agtaatccga agcagtctag caaatgtttc
     6061 ctttgtgatt ttcatcgggc ggtgttgttc tgataaaatg atcgaatgtt cattgtaata
     6121 agcataaggg gaatattgga atccccagct ttcaccgtct agattcattc gaataatccg
     6181 gtgattggtg cgggctggat agttcaagcg acctctatat ccttcgtttt ccatgcaaag
     6241 catgcaggta ggatagtcta cttttaccgc ctcgcgttct gctgcgattt gttttggatc
     6301 tttttctggc ttagataaat tgattgtgat ttccagttca ccatactccg actctgccgg
     6361 aaatttgatg ttttttgcga tggctcttgt tttgatgtag tcatttttct tacttaaatt
     6421 aaagaaatac tcagttgctt cctctgggct tttcgaatag tgctgtgcaa agaatgcatt
     6481 gacgactgaa ggtggcggtg tgagaaaatc cattaattgc gcttcaaaaa tctcttgttc
     6541 tgctggcaat tctgaaagaa caccattttc tcttgcctta gcgacaagct gatccaataa
     6601 gtctacagat tcttccttaa ctggtcttac ttcaatgctt tctagagcat cttctcctac
     6661 catccctaaa agacgattct gtaaatataa gcggtccatt tccatccatc cgccagcttc
     6721 gatcgcaagt gtggcaaaat cacaaatcgt ttgactgatt gacatatctc attcccctat
     6781 tcttcataac catttggatg gctgacatgc caatcccatg ctgttttgat aatcgctttg
     6841 atatctgtgt attcaggttt ccagcccaga atctctctcg ctttttcact tgaagcgact
     6901 aatcggctag gatctccagc tcttcttggt gctatttctg ctggaatttc ttttcctgtc
     6961 acttctctag ctgcttctag catttccttg actgagtatc ctttgctgct gcctaaattg
     7021 aagaagttgc tttcatttcc ttctttcaag tattccaaag ctaaaatatg tgcagcaatc
     7081 aaatcttcta cttgaacata atctctgata catgttccat ctggtgtatc ataatcgtct
     7141 ccataaacag ccaaggcttt tctttgacca agagctactt gtaaaatgat tgggacaaga
     7201 tgcgtttctg gtgtatgatc ttctccgata gatgcatctg cttttgctcc ggctacattg
     7261 aagtagcgca aagcgacata gcgcatgcca tatgcttggt cacaccactt catcattttt
     7321 tccatcatca atttactttc accatacgga tttttaggat tagctggtgt atcttctgtg
     7381 attggagact ctttcggctc tccataagtt gctgcagtag aagagaatac gattttattc
     7441 acgttgaact cgtgcatgac ttcaagtaag atttgcatac cgtatacgtt gttgttgaaa
     7501 tacatcaatg gtttttcgac agattctcct actaaagagc ttgcagcaaa atggattact
     7561 ccttcaatcg gttccttttc aaatacggaa cgtagaaatt ctttatcccg gatgtctcct
     7621 tcataaaaat gagcatctgg atgtactgct tgtttgtgac ctgtgagcaa attatctacc
     7681 acagctactt gatacccctt ttgaactagc tggtctacag cgtgcgaacc gatataaccg
     7741 gctccgccta gtactaaaat tgtcataaaa aacactcctt tgattagtaa aattgtgttt
     7801 gttgattcta tataatttaa aaaacgaagt ttacttttat ggtgaaaaca a
//
LOCUS       DAZKOB010000117         7751 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000117 DAZKOB010000000
VERSION     DAZKOB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.119"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..2439
                     /locus_tag="ACG8M6_002524"
     CDS             112..2439
                     /locus_tag="ACG8M6_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0458138.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2436..3350
                     /locus_tag="ACG8M6_002525"
                     /pseudo
     CDS             2436..3350
                     /locus_tag="ACG8M6_002525"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3423..3938
                     /locus_tag="ACG8M6_002526"
     CDS             3423..3938
                     /locus_tag="ACG8M6_002526"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0458139.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3935..4381
                     /locus_tag="ACG8M6_002527"
     CDS             3935..4381
                     /locus_tag="ACG8M6_002527"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0458140.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4531..5346
                     /locus_tag="ACG8M6_002528"
     CDS             4531..5346
                     /locus_tag="ACG8M6_002528"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0458141.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5552..6724
                     /locus_tag="ACG8M6_002529"
     CDS             5552..6724
                     /locus_tag="ACG8M6_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0458142.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6886..7380)
                     /locus_tag="ACG8M6_002530"
     CDS             complement(6886..7380)
                     /locus_tag="ACG8M6_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458143.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7377..7571)
                     /locus_tag="ACG8M6_002531"
     CDS             complement(7377..7571)
                     /locus_tag="ACG8M6_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458144.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2623 a         1455 c         1536 g         2137 t
ORIGIN      
        1 aataaaaaga gaaaaaaagc cccaaacttt ctatacatca ataaaataat aaaaattaaa
       61 tttagaataa cgtgtaaagg attatttgat gattttttat tgagaggagg gatgaaaatg
      121 attaagaaaa ctcaaaataa aatccaacgt atatgtcaaa aacattttgt ttctagactc
      181 aaagctgtat ctaaaagaaa atacttacat cccattatga tttcgatcat cttgattttt
      241 actttcttag ggagcttatc aagtatttat gtaaacgttt atacagaaac gtcaaatttt
      301 gctaaccaaa cgacagagga gacaaatagt ccaccctatt ctgaaataca gtccatagaa
      361 agcaccaatc agtctagcaa caacgagaca acctcaacca catcctcgac tcaactgatt
      421 gatccgacag aaaaagaaat cacagcagca aaagcagcag gggagaaagc ttattcagaa
      481 acaggtagaa gccagacatt tagtactgtt gcggccactg cgaatttcaa cggacatacc
      541 ataccagatg gatatacagt gatatatgcc aaagatggag tcattgttgc ggttaacgga
      601 tcgatgagtg cggataaaac tgcgttacaa ggcgtaacca aagcaatcgc ttttattgat
      661 aaccgtccag atatccataa tctgtttatt ggctccacag taaatggtac tactatcaca
      721 ttcccaaaaa gtatctatat gggggtggct accgctggaa atacgatcga ttcagttaca
      781 cccaatgcgg ataaaagtta ttcttttaca ccctcaacat ggggagggct tcctgccgga
      841 ggaggtatga gtactttttc aatcggtata cagttagcaa atgatattcc attcacgggg
      901 tactcgatca aaaatgggca gatcgttgat ggtaagggaa atgtttcaaa ttttccaacc
      961 tatcaagcag caacggttca ggtcaatgcg aatcggacag gaactttttc ttatacagtc
     1021 aatggtggag cagcaaaaat cggaacgtac gggactacat tatctggaat ccatgtgggt
     1081 gacaaagtgg ttatcactcc aacagcagta acgggctatt cttttgcaca aacaaacggt
     1141 tctttcacag ccctagaaag taacgtcgat acggtgacct atacaggcaa acaaacacca
     1201 atcactatta agtatcaaga ctattttgga cagatgattg ctccttctaa aataatgaat
     1261 cttacttacg gaagtgctgc acaagacctg acgactaata cgcctactat caacggatat
     1321 acctttacag ctgtttcagc gatagaaaca aagaaccaat cagcaatgag tgttttagct
     1381 agtttagata caaatggaaa tgtaatcgta aaggacgcaa atggaaaaca gatttctgaa
     1441 gtcatcctat attataagac aaatgcaaca gtatcaatca atgcaaatgg cagtaagtac
     1501 tatgatggtc tttctgttgt accgatcgtt aaatatgcct ttaatgataa aacagaatca
     1561 acatcccttt taaacaatga tctcagtatt gatcaaatca catggaatcc aactgatttc
     1621 aaagcgatga atgaaaaggg agttgaagta actgcgccta ctgaaattgg aacctatacg
     1681 tcttggcaat tgacaaaaag cggactagct aaactagctg ccagaacaaa ctatttattt
     1741 actatcgtgc agacatctga tgtctatacg attaagcaaa tcagcggtgc ggtagagctc
     1801 agtggcagta agacatatga tggaaaagca ggtgtcccgt caatccatgt caagctagca
     1861 gagggtgtca ctagttcgct tactccagta caagtggcac ttagcagtat ggattacaca
     1921 gcagatgcac aatcagcaaa aaatatggtt aatgctggca gctatacaat taaactaacg
     1981 aatagcggaa tagacaaagt caaagcagca aatcccaagt tgtcatttac tgatatagcc
     2041 aacacatcag gaacttatac catctataaa gctaacgcag tgattactgt cgatgacgct
     2101 gcctttaatt atgatgccca aagtcactcg atttcggttg gaaatctcca cgttaccggt
     2161 gttgtcttag gcgaatcatt ggattatacc ctaacagtta acagccggac agatattgga
     2221 tcacaaatag taacgattag tttaggcgaa ggaagtgtaa acaataatta tacgattatc
     2281 gcaacagata ccgctaagtt gacgatcaat ccgactctct tgcttccttc gacaggtggg
     2341 ataggtctac tgccctttgt ctttttagga ctgatcttta ttttttctgg cttcttttat
     2401 tttatccatc gtaagaaaaa agcaggtgaa cagcgatgaa aaaaacaaaa aatgtacgtg
     2461 tggatatatt tgctgttctc ttttttatcc tactggtctt ttttataggt tctccgactg
     2521 tgtcagctag agaatcacct attacattta ctttgcataa tcgaatgttc gaaagcgata
     2581 agatgccaag tatcagtcaa aacgacggaa taaatatccc ggataacttt caaggagtaa
     2641 aagggttgaa cggcgttact ttcgctatat atgatgtctc ggatgaattt tacaagctgc
     2701 ggtcagaagg aagtagtgtg gaagacgcac aaagaaaact agcacaaaaa agtgatagtg
     2761 aaaaaatact ggcagaaaat gtgacaaaaa cagtagacga ggaagagggc attgcttctt
     2821 ttagtgcatc ggacaaagat gagcaaggtc gtgacgctgt ttaccgattt acggaaataa
     2881 agacttcaga ttaagtaaaa gaaaaatcag caccatttgt cgttgtgctt cctgtgacag
     2941 attcatctaa tcataaattg acgaccatcc acttgtatcc aaagagcgaa caaaaaatac
     3001 ctgctgcact cacattaaca aaaacagtag agaataagca gacagatttt gcagatgggg
     3061 ataaagtccc ttatctcatc accgtaacaa ttcctgagaa catcaacgag ataggaacct
     3121 atacgatcaa agatacagct gatcctcagt tatggctaga accagaaacg atcgattttt
     3181 tgattggtgg agacaagatt catacatttc acacacaaaa aataaaacat ggttttgtct
     3241 tatcagattc tggaaaagat cttagttcgt ttgctggaaa aaaattaacg atccgttacc
     3301 aaatgacatt gaaagaaaac agtgaaaaaa cgacatttga taacgatgta cgtttgacga
     3361 ctgacaatca aacagtagaa acacatcttg ccatccaaac aggaggcaaa caatttgtca
     3421 aagtggatcg gcaaacgaaa caaaaactag cagatgctca atttctagtc aaaaaaaatg
     3481 accaatactt agcgcaagaa aacgggataa atcattgggt agccaaagat gattcaagag
     3541 caacgatttt tacttccagt aaagatggaa cattttctgt ccatggattg tcttttggaa
     3601 gttacgaact agtggagatt aaaccgccta aaggatatat attgagtcaa tcaaccattc
     3661 tctttgaagt tgaaaaaggc agttatgatc cgcttcatcc tataccattg gaaatcatca
     3721 acgaaccaaa agcaaatccg acgaataatg gaaaaacgcc agttcagatg cagacaaatg
     3781 gtccatcaaa tgaaaaaaat ggaggaagct ttccaaaaac aagcgaagag atgcttggag
     3841 gtttctcaat tttagggtta ttgctagtcc tttcaacagg gacagcttgg ttttataaaa
     3901 aacaacaaaa aggaaacaat aggagggaaa taaaatgaaa aacatcaaaa caaaaatatg
     3961 gacgttttta ggaactgcta tcatgctgct tcctttcgtt ctcggcctag gaactgcaga
     4021 ggtttctgca gccgtatcac ctacaccaga aaatgttacg gttaatttgc ataaattgaa
     4081 attcacttca gcaccagaaa atcaaataaa caacggaacg gaattgacat ttccgaatag
     4141 cgaaccgttg aacggcgttg aattcaacgt atatgacatc actgctacgt actatccaag
     4201 taaagacaca gcagttccag cagacgctac acccttcgca agtgtaacga cttctggtga
     4261 aggattagct aacctgactc ttccaggtaa atctgatggg aaagatgctg tatacgtctt
     4321 tgtagaaaca ccaaagcccg gtgtcgaaac gtcgccaaat atcgtgttaa gcttacccta
     4381 gaccctgaca aacctttaga cacgattcac ctatatgcta aaaatgtcat caaagaaagc
     4441 aatatcaaaa tccagaaagc tgggaacttt tcaggggtag ataagttagc aggggcgcaa
     4501 ttccagatca aaaattcaga gaatcaattc gtgaccggtt ttgatgaaaa cactggtgtc
     4561 gctctctatg atcctgctga gtcagcgttg acattcacaa ctgatactga aggatccatc
     4621 agtgtcaatg gattaataga cggcaattat actttagttg aaacaaaagc accagatggt
     4681 tatcaattaa ccaatccaca aaaagaaact acttttatag taaaaaacgg aaaattgcaa
     4741 caaacaaccg gtatcatcca tggcgcagat gaagccaatc ttgggaatcc aaacggtgta
     4801 gtcaatcggg aaaatacaac tacggataac acgattactg tagaaaatgt ccaaaactac
     4861 ggtgatttcc acttcatcaa acaagatgtg aacacgcaag aaaaattagc cgatgcagaa
     4921 ttttctattc taagagcaaa agatccaact gttaaattaa tgaaaaaaat agcaaacctt
     4981 gcctcaggag aatacccata tgcttggtca gatgaagtca caggttctga ctgggaagaa
     5041 gtgaaattga tttctgattc agaagggaaa ttttcaatcg aagggctgaa acaaggggca
     5101 tactgccttc aagaaacaaa agcaccagaa ggctacgcat tacctaagaa tgaagctgcg
     5161 tacactgaat ttacagtaga tgatgatcca acaacttctg atgaaacaac gatcgataac
     5221 caacctaaag gaatcttgcc ttcaactggt ggtatgggga tcatcgtatt catcttggta
     5281 ggtactgctt tagttggagg agcagtgatc tatttcaaaa aacgccatca agaaacagaa
     5341 gcttaaacta ttggaaacaa aaatcatacg ataaataaac gatagtgatt caaatgggga
     5401 gaaaggttgt gacaagtggt cggtctcttg gtattcgctc aacaaacggc aaaataggag
     5461 gaactatttt gccgtttgct tcgctaatac tcaggtcaag gcgctgattt cacaacctct
     5521 tcatatacat attagaaaat aggtggacat gatgaataaa agcagaataa gaatactgga
     5581 catcttcatg gcaatcattt tagtcgttgg gatcgggatc ttttcttatc cttttgtaga
     5641 agattcactg aatgattttc ttgcccagca aatgatcatc cattatcaaa agcaagcatc
     5701 gaataagaat tcagaggaaa taaaaaaaca gcaggaaaaa atgacaaaga aaaaccaaca
     5761 gttggcagag caaaacgtat caccagggat aggcagtttc aaccaagcag ttgatgcaaa
     5821 agcattaaaa gatttaccta gtaatgcctt ttttatggaa catatgcttg gagtcatcga
     5881 aatcccaaaa atcaatgtca gcttacctat attcgatcag acaacggaaa tatttttgca
     5941 aaaaggaacg tcattattgg aagaaagttc ttatccgact ggcgggaaaa acacccatgc
     6001 cgttctttct gggcatcgtg gactcccaga agcaaaatta tttacagatt tgcctaagtt
     6061 gaaaaaagga gaccagtttt tcatccagat caatggaaag acattggcgt atcaagtaga
     6121 aaaaatccaa gtggtcttac ctgatgaagt agattctttg ggtatccaaa aaggcagaga
     6181 tctcgtgact ttgcttacct gtactcctta tatggtcaat acacatcgac tgcttgtgac
     6241 ggggcatcgt attccttatc aagcaaaaga ggcaaaaaaa gcgatccaag gaattgatca
     6301 atggaaaaaa tggaaatttt ttatttggtt tatcggtata ctacttggat caatcggctt
     6361 agtttggcta ctcatcgctt atttagattc tttggccatc gctaagcgaa actatccttt
     6421 atctttttat gtaaagaaca caaacggacg accaatcgaa ggaatggtct tttctgtaaa
     6481 aacgctcaac ggcaaacact atattactag agaaaaagtt ccatttgtga aagcttctga
     6541 tgaatacgga ctagtgagat tttctgattt aaaaggcagg aactatcgct tgcaacatga
     6601 ggagctcctg cttaaaatcc atgtaaaaca taaacattcg aaacagtttt caatgaaatt
     6661 gaaaaaagga agatataaac tgcgaaaaga aaaagaggtg tactatttga ttgagaaaga
     6721 atgaatgaag atgtccaaaa aagcagaggg aacagactaa atgaaaaaag agagaagctg
     6781 acgaaatgga cgtgtaaact gttatttttg gtttactatc tggaacgcca tcttctattt
     6841 ttttatgttt cagaagaatt ttattattat agtataactt ttgaattagt ttccataaca
     6901 tttggagcaa ggggttaaac cacgagcttg agcttcgctt aaagtggcag gggtatacgt
     6961 accatttcca catttatgtg tatggtattt tgaaccggtc ggtgtgacat agacagtttg
     7021 ttcattattt tgctcaggag ctgcggcttg tgcctgctga gaggcctgtt cagctgctga
     7081 ttgctgtcgc gcctgttctg cttgttgctg tgcgattgct tcttgcctcg cctgttctgc
     7141 ttgttgctgt gcgattgctt cttgttgcgc ttgttctgct tgttgtcgtg caatttcttc
     7201 ttgctttttc cgttcttctt ctgcttcttt tgctttgatt gtggaatcaa ttgcagcaag
     7261 acggcttgtc aactcttgtt tgttttcagg tagcgcttgg atagctgctg aagcagttgc
     7321 atagttttct tttgtaggat ttgcttcagc ttgtgacaaa gcagattccg cttctgtcac
     7381 tttttttcta gtgcagcttt ttccgctttt tgtttctctt cttttttctt ttcaatttgt
     7441 tcttgttctt tcgatgaatc tgccgtactt tcggagatcg tggtactgct gctggaagcc
     7501 tgatctgaac ttgtgctttg agtagtagac gtagtagcac cacacccact caacaaactt
     7561 cctgcgacca aaaacaatgc aactaatttt ttcgctaaat ttcctcctta agtaaaaaac
     7621 aaccgttttc aataaatgtc cttttcaatt gtttacttac ttgttataat aattgtgtta
     7681 tgaatagtga ttgaggaaac ttaatctaat cccgtgttga tacacgggat tttttgctat
     7741 agttctgtta t
//
LOCUS       DAZKOB010000118         7648 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000118 DAZKOB010000000
VERSION     DAZKOB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.120"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1292
                     /locus_tag="ACG8M6_002532"
     CDS             138..1292
                     /locus_tag="ACG8M6_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0458145.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1265..1708
                     /locus_tag="ACG8M6_002533"
     CDS             1265..1708
                     /locus_tag="ACG8M6_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458146.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2842..3810
                     /locus_tag="ACG8M6_002534"
     CDS             2842..3810
                     /locus_tag="ACG8M6_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0458147.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3820..4308
                     /locus_tag="ACG8M6_002535"
     CDS             3820..4308
                     /locus_tag="ACG8M6_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0458148.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4604..4885
                     /locus_tag="ACG8M6_002536"
     CDS             4604..4885
                     /locus_tag="ACG8M6_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458149.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4887..5303
                     /locus_tag="ACG8M6_002537"
     CDS             4887..5303
                     /locus_tag="ACG8M6_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458150.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            complement(6058..7206)
                     /gene="tnpB"
                     /locus_tag="ACG8M6_002538"
     CDS             complement(6058..7206)
                     /gene="tnpB"
                     /locus_tag="ACG8M6_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFQ0458151.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(7223..7627)
                     /gene="tnpA"
                     /locus_tag="ACG8M6_002539"
     CDS             complement(7223..7627)
                     /gene="tnpA"
                     /locus_tag="ACG8M6_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0458152.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT         2704 a         1323 c         1242 g         2379 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatttaaca caaaggcgtg attattttga aacccaaaaa gaaaatttat ctaggtctcc
      181 tcttttcctt gatgattgga atttttagta taggaattac aaatacctat gcttcagaaa
      241 attctaattt caatatcaac tcccaaccaa ttgaagaaca agcaattaat tttgcgaaaa
      301 ctgattttga acactccata acttcaattc ttttaatgga aaataacacc gaaactgata
      361 ttaactatac tcttggttcg ccatttaaaa tgctcgatgg agaaaatacg gcaaatagtt
      421 ctattttctt tccagtattt tcagacgctg ataatacgat taaatatgtt tataccgtca
      481 aaaaaaatac aaatggagaa tattcaggaa gtatttcaca atttttagca aaagaacttc
      541 aaaatctagt agatagtaat ataacagata caaccttatt ttttcaagat aatagcattt
      601 attattccga taatggacaa attaaactac tttggaaatc acctttaagt acagaaaaaa
      661 atggtgtttc ttcccttaca gaaaaaaatg atcttatatc cgtaaatata aaaaataaaa
      721 ttaaaaattt ttctaaaaaa caaaattttc ctgcggttaa acgtgtaaaa agtgtctcct
      781 ccggagataa ctttattatg attaattggc gtatagcaga aacccaaaca agttttccgt
      841 ggtgcgctgc ttacgtagaa gctgctatct tatgtaacaa aacagatgta cgtgctactt
      901 cagctagagc cattatgcat tatacctatc ctaatttaaa caacagtcaa attgaatcaa
      961 aaagtcttaa tcaagatcaa ataataaaat atgcaaatta ctgtggtgtt tatcctaaaa
     1021 aaatagcacg aatactaaac ctttcagagg tccaaacaca actaagaaaa ggaaatgcca
     1081 tttatatggg aactgctggt tatggaaatt atagtaacag ccgtcatgca cttgcattat
     1141 ttgggtgggt caatcaaggt ggttatcaaa cttactatgt gtggaatcct tggtataact
     1201 acccaactgt aataagtgca aatgcaggac cagttacaat tcctgtacct ggaggtggtt
     1261 ttacttggga caataccatt acaaattggt aaataaactc attgctttag ttatgggtct
     1321 agtgttagtt cttttcttaa ctagttgttc aaaaaaacaa gaaatacaaa agactgatct
     1381 gattcctttt ctaggaactt ggcaaactaa agatcattca gaacgtctag taatcactaa
     1441 tttaaatact tcaaaaaata tagaacaata taccatctta gtaaaagaga acaatcataa
     1501 aattcaaatc attgataaaa atttaaatat aaatgaacaa gttttcattt acaaagcaac
     1561 aaataattta ctaacactgc gctcgaataa aaaacaatta gattacactt ttagtttgga
     1621 ttctccaaaa aagctcgtat ttattttgac aacgaatgat gggacagaag ggacagctaa
     1681 accaattgaa ttttttaaag ctgattgatg aatagttgtt tgatattaca aaacaataat
     1741 ttcaccgaag gtgaaacaag cgaaacttta caaaaagtgg ctagcatttg ctagcctttt
     1801 ttgtattgtg gagcgtgggt tttgaggggg attcaagggg gagcttgcgc ccctttgaca
     1861 agcggttatt tgcaccaaat acgtaggtat ttggctagca aatatagagc ttgccaagcc
     1921 ctttagtgtg ttttgtagaa agggacctta tagtcacttt cacattacaa acgatacgtc
     1981 ctatggacgt atcaaaaggg tgggacttat gagcaaatta aagcaaaatt tggctagcaa
     2041 tccttctaaa aaatacacct attgatcaga gtcaaaataa atcagttttc gagtgagcga
     2101 atccaaattt gcaactctga agctttgcaa atgagtgtgc cggcttccgt aaaagtctag
     2161 gtaacattag tcgatatata catagggctc taatttgcgt tttaaggcgt tttaaacgtt
     2221 ctggtatgta attataccaa agcgacttta aaacgcctta aaacgaattt tagaggttct
     2281 gtagaacgtt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttaaaaaca
     2341 agataaggga ttttatctag acttgtctta aaacgccgta gatgagcgct cagagcttct
     2401 aaaaggactc cttacgaact gtttttaagc aaaggagcct ttaggcgact gcgcagttta
     2461 atgtgagagc gtagcgatca cacctttaaa gcgaccgtag ggagcagatt cccagtttag
     2521 gaaatttcgc gaacctgttt ggagaaaaaa agtaggaaaa ttttaggcgg gaaattttgc
     2581 ccccctcttt tataatctct tttataacct ctttttaaac atctttaccg tcggctgtat
     2641 ccttactctc ccaaggtgtg cagaatcttt taggggtctt tttgtctgtt tttaggggtc
     2701 tttttgtctg tttttagggg tctttttgtc tgtttttagg ggtctttttg tctgttttta
     2761 ggggtcttta attagggggt tgacccccat tagaaagctc tttataattt tgttaagtag
     2821 ttaatcttga aggaggattt tatggcaaat gaaattgtga aataccacca tgaattaaat
     2881 acgattcctt tacgcaaatt tacgtcagta gaaatgaatt tattcttctc tataatatct
     2941 agaatgcgtg atgtaggcga taaaaaagta caatttacat tcgaacaatt aaaagattta
     3001 agcaattata aagcaactgc aaatgttcga ttcatagatg atttagaaac aacgtatgac
     3061 aaattaatgg atttacgttt tggtagaaga agtgctgacg gtttgcaacg tgaacgattt
     3121 gttttattta atcaatttaa aatcgatgga aaagctgata ttccttttgc tgaaatacaa
     3181 gtccatgaga aagccttacc cttacttaat aatcttgaag aatgggttcg atattctctc
     3241 caacaattca atgaattaga aagcagttat tccaaaacca tgtttagatt attaaaacaa
     3301 tacagaacca aaggaattgc caatttttca aaagaagact ttcatgaatt acttgatatc
     3361 cctaaaagtt atagagaaag tgatataaat aaaaaaatta taaagcctat tcgccaagaa
     3421 ctaaccgcaa tcttcaaagg tttgacaatc caaaagagat acggaaaagg acggggaaag
     3481 cctgtcattg gctatcaatt tacatttaaa cctgagataa aaaaatcaga cgactttaac
     3541 aaacatgctc caaccaaaga agaacaaaat aaagctttgg aactagctaa tcaacaggtt
     3601 caatctgaaa aagaacctac ttcattacaa gaaaaaatca aagaacaaaa acgaaaatta
     3661 gaacttttac aacagttagc agaattagaa agaaaacaaa ataccccaag taagaaaatt
     3721 aacaaacaaa tagcagataa caattccgat attaccccag aatttaaaga aaacttacta
     3781 gataatctcc aaaacttatt taaagaataa ggggttttta tgacagataa aactatcaaa
     3841 gaattagcag atattctagg tgtttcaaaa gataaagtga agtatcaact gaggaaatta
     3901 cctagtaatt ttacctacaa aaaaggaaat attacctacc tgaaaaaaga agcagtaaca
     3961 acaattacaa aggttttatc aggtaatgaa tcgggtaact tacctagtga attacccgat
     4021 tcattaccta ccgattacct actcaaggag attgaaacta agaataatca tattgaagaa
     4081 aaagataggc aaattgaaaa acttcaaata gctttagaaa cgcagcaaaa attactcgac
     4141 cagcaacaac aactcaccct acaagccaat caacaaatca aaaaacttca cgaacaactc
     4201 cagctaacct atgcagaaga atcttcagaa aaagaacccc tttctgttac tgaagaaact
     4261 caaaaaggac aaaacaagga gaaaaaatgg tggcaattct ggcaataacc caacaatttt
     4321 tattcataaa aattgttggg ttactttctt tttttgcttt tacaagcata gcgacatgat
     4381 ctcacgtcgc tattttttaa agagcataag gaaacgcggt atcactttct tacgaaaggt
     4441 gctaggtagg ctgtgcctac cacgtttcta agaaaaaaat ttgctatcac tttttcgtca
     4501 ttgtggatag cttatgtata gtgtttcggc aaaaaacagc tatacaaata ctatatttgt
     4561 atgatataca taaattatac acaaatgcga aaggaattct tctatggaca ttttagttcg
     4621 gaatattgat ccaaaagtta tttcagaaat agatagacgt tgccaagaac tcacaacaaa
     4681 aactggcaaa aaatggagcc gtaatgcgta cctaaaagtt ctaatcgaaa acgattttga
     4741 ccaggcactt aggcattaca aaaaagatca attcgattta ttactcgaaa aatttatcga
     4801 tatacaagca aaaaatactg aaacattgaa cgactatgtt catacaaaca accaactcat
     4861 tgaaacactt atcggaggaa tataacatga gaaaaacttc tgttcaaaaa cacgttaccc
     4921 tttatcccag tcacattgac gttttagatt ccatcatcga aaaaaatcca gatatcaata
     4981 actactcgga agccattcgt tttctatgta tgagttatga acctaatact aatacgacaa
     5041 ctaccagcaa ccaagcaaaa ctaaatgcga tgggaaaaga aatttctatt atgtccgaaa
     5101 tcattgcaga atttgctgat ttatatctaa aagatactgg tgttcttatc ggtacaaatt
     5161 ctaaagtata tcaagaagtg aaagaaaaaa ttgaaaatca actcaatccc tcccgacgaa
     5221 atcaacccaa aaatcgtcaa aaagaaacca atcaaacatt cacttctaga aattttgacc
     5281 caacaaactt tctaaaaaga tgaaaaagaa accgaaaatt ttttctattt ctcagtttct
     5341 tttttagggg gcagtcttcc agcaagattt gcaagttacc ggtaacttgc acaaaaaaag
     5401 aaaaatccgc ttgtgcgaat ccttcttttt gcattttggg gcgctgcccc aatctccaaa
     5461 tatcggttat ttaatagcgt ggtaactcaa aaaaatagaa cgtactctga aataaaaaaa
     5521 gactgctcta acgagcagcc aataaaaaat aatgagcgtt tattcttgaa aaagcagcgc
     5581 gcttatgaag cgctgccaaa aatttaccat attttatgaa aaataacaag tttctaaaaa
     5641 ggaatcggtt aagattcaca aagaatcgta aacagacaat agcggaaaat caagcttccg
     5701 aacgtacctt ttagctagac caatgaaaaa tcacaaagtg agcccctgta tcggttgata
     5761 caagggctac ttggcatact cgacattatg tctactagct cgataaataa cgattatgtt
     5821 cccaaaacaa tattttatga actataacaa aaatttgtta tcgcatattg tatattttaa
     5881 ttataggtgt atatcacgtg aactactcac cacttagcta aacctgacgg ttcttaacgc
     5941 ttgaagtggg agcttcttgg gaatagagca tacttgatag cgtatttctt tactaacagc
     6001 ggtgtctctt atttaccaag ctatccccgt agttcctacg gttcttgatg attggatcta
     6061 agccaatccc atagaatgta gtcttagacc ttcggtcaga atattgatac tagcattgat
     6121 atcacgatca tgatgagtat gacaaacagg acaagtccat tctcgaattt caagagattt
     6181 cttgccatct ttatgtccgc actctgaaca aatctgacta gacggaaacc atttatctac
     6241 tttgatgatt tttcgtccat accagtcagc cttgtattgt aatttagtca caaactttga
     6301 ccaagaaaca tcagaaatac ttttagctaa tttatgattt cgcaacatac cttttgtgtt
     6361 taagtcttca atacagataa tatcgtgatt tttgatcatt tctgtactca acttattcag
     6421 aaaatcagta cgttgattca ttactttttc atgcaatcta gctactttgc gtttttgttt
     6481 ttgataattt ttagcttcaa atagattggt acctttattt ttagccaata aagcacgcct
     6541 agacaatttg cgttgttcac gctttagttt cttttccatt ttggacgtga atttattatt
     6601 atcaattttt tgtccatcag aaagaatcgc aaagtccata atacctaagt caataccaat
     6661 tgtggaatta gttttaggta attcgataat ttcttcttta cacaacaaag aaatatagta
     6721 tttaccgcta gaatgacgtg atattgtagc agatttgata atcccttttg gttgtctatg
     6781 aagcttaatt cttactaatg acttcaattt aggaactttg atgaatttac tatcaatcaa
     6841 agcaactgta ccattttgat tattcgttgt ataactctga acaggatttt tcttactttt
     6901 gaaacgtgga aatccaacgg atttatcccg aaaaaaattc ttgtatgctt tatctaaatt
     6961 aagttgggca ttggctaaag caaggctatc aatttctttc aaaaacggaa attctttctt
     7021 gtatttagct ggtgtcggaa aagtcatttt tttagaagaa tcatttttaa cttcttcata
     7081 cgcctttttt cggtcatcaa gcattagatt gtagacctta cggacacaac caaaagattt
     7141 ggcaaagaag atttcttgtt cctctgttgg atagattctg aatttgtatg cttttagttg
     7201 ttccataaag ttcacctact ttctatttat gattgtttcc ttgattttgg atatacttct
     7261 taatcacatc aattggtgcg ccaccagttg tcagaagaca aaaactttta gaccagaaca
     7321 tttctttcca aagaaattgt ttgactcttg gaaagtcacg ttttatcaat cttgagctgg
     7381 cacttttata ggcattgatg aattttgtca attctgtttt aggttgagct ttgaacataa
     7441 tatgaacgtg gtctttatca tgattccact ccactaaagt aatgtgatac gattctgaaa
     7501 ttctttcaaa agtagttttg gcataatcag atatttcatc atcaatcact tgtctacgat
     7561 atttcgttac taaaacaagg tggtaataaa gaagaaatac tgaatgatta tttgtatcta
     7621 attccatgtt atatcaactc atttcttg
//
LOCUS       DAZKOB010000119         7648 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000119 DAZKOB010000000
VERSION     DAZKOB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.121"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(213..548)
                     /locus_tag="ACG8M6_002540"
     CDS             complement(213..548)
                     /locus_tag="ACG8M6_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458153.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(723..1217)
                     /locus_tag="ACG8M6_002541"
     CDS             complement(723..1217)
                     /locus_tag="ACG8M6_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458154.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFVL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            complement(1214..1441)
                     /locus_tag="ACG8M6_002542"
     CDS             complement(1214..1441)
                     /locus_tag="ACG8M6_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458155.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(1608..3578)
                     /locus_tag="ACG8M6_002543"
     CDS             complement(1608..3578)
                     /locus_tag="ACG8M6_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458156.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(3593..4459)
                     /locus_tag="ACG8M6_002544"
     CDS             complement(3593..4459)
                     /locus_tag="ACG8M6_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458157.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(4449..4682)
                     /locus_tag="ACG8M6_002545"
     CDS             complement(4449..4682)
                     /locus_tag="ACG8M6_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0458158.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(4694..6850)
                     /locus_tag="ACG8M6_002546"
     CDS             complement(4694..6850)
                     /locus_tag="ACG8M6_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458159.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(6870..7115)
                     /locus_tag="ACG8M6_002547"
     CDS             complement(6870..7115)
                     /locus_tag="ACG8M6_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458160.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(7201..7539)
                     /locus_tag="ACG8M6_002548"
     CDS             complement(7201..7539)
                     /locus_tag="ACG8M6_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458161.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
BASE COUNT         2203 a         1506 c         1019 g         2920 t
ORIGIN      
        1 caaattaaac taattagtta tattctcaca atagcattta ataataaata tactgtcgct
       61 tgatttatat atttccctct tctttcttta gaatctctct ttattttgat ataataaaat
      121 agaaatcgca ccaaacaatg aaatggcgga aaacatttca ttgaaaaaag gattgaacat
      181 aatgttcaat ccttttttat agttataatc aattaacatt cggtgtgtga aattgaatct
      241 tcgatgcttt cacatggaaa attattgatt ccttcttcac tcatttccca tgtagtatag
      301 aaaggagtta actgtcctcc atactcctga ataataaaat attttccatc attgatatgg
      361 ttacaacagt acactccaat agttggatac tcgactacta aaaattggtc tggatcaaac
      421 tgttcttttt tcaacatttc tcgtatttga aacaaatgat ccttctctaa gcaatcaggt
      481 aattccttta ttctttgcgt tactaatgac ccctctatcg ctttccattc atttaaatat
      541 tgtttcataa caattcattc cttttatgtt aactattttt cctaggaaat tgttttaaaa
      601 cgccactctc ccagaaaacg taagatttgg ttagctggaa tataccttcc agctaaccac
      661 aattttccat tactatattg taagcttgct ttcacttcat aaaaattgct gatatactct
      721 tttcaaatcg aactcttcct atctgagaag ggtatttctt gcgctagccc ctcataagtt
      781 ccatcttcat tttcccaaat ttctacctga aacactgaat tattggctaa tttttgttgc
      841 aagagataat agggttcttc ttcatacttt tgatcgactt ctttaagaca ttcaatagca
      901 tcaatatcaa tattttgaaa gcccaatttt tctaggtaac aatcgtacca agtaccccat
      961 gtattatcca gaaatgagac aaatgcatga tccatatcat ttgctataaa agattcatct
     1021 tcccatctga agaggtaatc ccactcacca tttgcaattt tagttctgat ttcattttct
     1081 ttttttaaca caaaaggatt acaccaacaa tgctcatgtc gcatccaaac aaaaaattgt
     1141 ccattctcat ctattttaaa ttgactgaga tcacctttca atctaaatac acggttcttt
     1201 tctggtaact tattcatgct tcaaccacct catactgttc tctcatacct ttgacttcat
     1261 taaaaaaaac tctcgctttt tcttcatcag taaagttttt atacaactga ttacaataat
     1321 gaggtttttg aagtttctga atacctatac taacttcaaa tccttcttca tgttgaatta
     1381 ggattacttc tgtgtctcta cctagaaata ttcgatctaa aatttttatg tttggtttca
     1441 ttatttaacc tccactataa ttatttcact caataaggtt tcactttatg aaatttctat
     1501 agttctcttt tcacaaaacg taccatgttc tctaagactg tttttagtct caaatataca
     1561 tgttttttct tatcctttcc ttatacactc aaaatcaaag ataaaactta aagaataaac
     1621 tctccaataa caaaattatt agtcagatcc actggtctcc tgatctgaaa tttattactt
     1681 cttcctgttg tcaaatactg tctcaaatca acagaaagtt tttgatcatc ttctcctttt
     1741 ctagcaggag caactttttt ttcatacttc attaactctc cactggtcat ctttccaatg
     1801 atagtgtaat taaaatcact ttcaaaactt tggtaaattt ttccatctgt attgaatata
     1861 tcagctgttt caatatcttc tttgtattca ttgtcttctc ctgaaccgat tgtatcaaat
     1921 agaaaggcaa gacgaattcc atcttcttca agcattgtta atcttttttt aattagccca
     1981 acggtttcaa tagataactt atcgaagccg tgaatcataa caatatcatc tttttttgct
     2041 tgtgatgtga tgaaatcaaa ggtatttaaa aattgagctt ccataacatt ggtttctgct
     2101 cttagatcac ctaagttata gtaaaactgt aatttgtcac ttttaccgat ttctttcagt
     2161 gtcgttcttt gattaaacat cctagagaag ctgtctaatg ctgttctcaa tgtgtctttt
     2221 aatacaataa tacgatcgac ttctttttca gtaccagatt ttttggcttt tgtttcagca
     2281 ttagtcaaat agctaataaa atccccaaga actggcattg tatcactact gttcaccaaa
     2341 agttttgaac gttcttcata attaatatcc caaagtttct gacttatata aaagttacct
     2401 aattcttttt tcaataaaac tctagttgca gaatctaatg ttctgcctgc taacaaataa
     2461 aaaatctgag ttagtttatc gagattccgt ctatataaat caccaatgtc cttttgatta
     2521 ccaaaaagct ccaaagagtt aagaccttct tcagccaaat taaccttttc tattacactg
     2581 tttaacgaag gtttggcaac aaatgttcta ccagttggat caccaaagta atcaaattta
     2641 tttaagacaa tatgaaaagt tcgtttatga tttgccattg cattattagc aatcaattgc
     2701 ccccaaagtg atgatgctga gattttttca tcatagcctt taattgtagc gtttttgtgt
     2761 gatgccacta aaactttact tgtaaaagaa tcatccagtg ccataaaagc tacaccactt
     2821 gcgtagctag tagacatagt gccgatagcc catccattcg catcatctaa acctttgcga
     2881 attgcatgat catttcctgc aaaatccgaa ctcatagacg tatactcata gacattcccg
     2941 attggttcac ttaaaagtct atcaaacaga tcacgttgat ctcctccata tgacataagc
     3001 tttaatccat acattttttc atcatacaac cgttggagtt taattagctg ttcgctgatt
     3061 ttattaggag aatcggatac taataggatt tctagcttcc aatcaataat tttatttgcc
     3121 atgctgtcta gtaaagatgc tgtattcaaa tccataatcc gcataacagt tttgctaatt
     3181 tcagcatctg aattttttcc ttctaaatta ttttcataac tattcgttga tcccccaaca
     3241 ttctcgtgca ttatttcttg ttgatctttt tgtgacactg gcttatctgc ttcaattaga
     3301 taggctttta ccccttctac tctcacttca ggaatagctt taaccatcca accaaatcca
     3361 taatttctat tcattccata agtattcact aatttcacaa ttgttcccca ccgcttacct
     3421 gtcctaatgt aagatggtgt aaattgataa ccaactaaag atgttaaatc actaacaaag
     3481 gtattattca ctacctttga atttctagtt ttttgttctt ttttagtttt tctctttttt
     3541 ttgaaattta atagttgtga tctacccata atttccattt aatctctcac ctctatttca
     3601 gtgcataaat atcagataat atttcttttg tttgtttagc tgacaactga gtgacactat
     3661 aataaagtcc tctactcgta tagttttcta aacgttccac accttcatcc aataccatct
     3721 tagattgaga tttcaagata agatattgga ttactgttgt tttcacacca tcaatatatt
     3781 ctgatacaaa tcgttcttgc tgatctatta tgtcaaagac tgctttgtta ctccttgcat
     3841 ttcgtttttg tattttcata tttcgagttt gtctttccgt attctgaggt tgagatgaag
     3901 tgattttgat ttcagctaag gaacgatctc tggtattttg atattgtatt gatttcgatt
     3961 cctgagccat aacttcttct ggaaaagcgg ttagtgatgt agccccatct agattgaaca
     4021 ttaatgctac actcccatct cgatatacga ttgttccatc ttgtttcact tttaaaatct
     4081 ctgtgcctcg aaatggtttc tttttcccca tagatactct tgctgaatta actactgatt
     4141 tgaacagtct aatagaacca gaaatcaatg aaccactaga tggatcaata aacatatact
     4201 ggctaattag aataagaaat ccaattatca ttaataacgt tggaacaaaa ataaatttca
     4261 gtttaatcgt atcagaaact aagtaaacaa ttgatccaag aacaacgact gcaacaacta
     4321 tagctgatac aattaatctt tttgctgtaa cagaataggt gctattgaca tgcttttcaa
     4381 gccattcaac tctgacaatt gtttcagaag gtttttgatg taagttatgt ttaaatccat
     4441 agtttgagtt atctgccatc agaaatactt cctttcacgt tcaacttacg gtaaacacct
     4501 acagttatca ctattgcaac aataagcaag ccaattaaag atattgactt gctcattgtc
     4561 tcgttcgttt gtggtagttc atttacctga tacacgagct gtgcttcctg taagtgctga
     4621 gaagaggtca tgttttcttg attgttttga attactggat aaactatttt ttcttctcgc
     4681 ataatagccc tccttagtta ttatcagata agtgagcttg aattttgtac tgatttccgt
     4741 ctggagtttt cacaactgct ttttctccat caatagaaat cacttcagcc ttagttgttt
     4801 tcccatcaat actgatgaca acatctttcc caactttgaa ctctgtagtt ggtgttgctt
     4861 catagctact atttgttgaa gcagagttcg tatttgtccc aatcgaattt tccactctcg
     4921 aaatagatgt ttgagtttga gcctgtgctt gttgattatt atgactaata aagtaggacg
     4981 atcccaagat cgttccgcaa gccattgcag cgacagaaat gatagtagcc atctttttac
     5041 ttgtaaaatc acgttttgaa cttgattgat tttctgtagc aactgctaat gatttcgttt
     5101 tttctgccat ttgagctaga tcacttcggg aacgagataa gtcttctctt aaagcttcac
     5161 tttgttctgc caacttttcg ctatttaatt tataaccctc tgctaaatct ttgacctctt
     5221 tcattcgatc tttatattct tcgatagttt tttgttgctc tacaagtttt gtttctaatt
     5281 ctttcgtgtt gttagctacc tgcttttcaa cttcttcttt gttcgcaaaa tatttgacaa
     5341 ccacatctgt tgttttctct tttgtatcat ggaactcagc ttcattttct gactttgttt
     5401 tttcgacaat ctctaaagtt ttttcttgca cgtctgttaa tgatttttca atttctggtt
     5461 tcaatgaatt gataattcgt tccaccacaa cttcttttgc ttcttggctt tgtttcaatg
     5521 catcgttttt aatagattca attgcttctt ggtactgttg atcaagactt tcttcagttg
     5581 cttttctata ttcatcttct tgttgtttaa cttctggtaa atgctcagca tcataccgtt
     5641 gctctagttt ctttagttca gcatccaaat aagcctgttt cgccatatca taatctttac
     5701 gaatgatatt ggatttttcc atcactaaat catcaatttt agatgcttct gcttcgtagc
     5761 tctctttagc aaccgatagt gtatttgtaa actgattttg accatttaat gaggttgctt
     5821 cctctatccg caaaagctca tcagctaaca tttgctttcc tttgaggatc aattgctcct
     5881 tcgtcttatt taattcactg ttgattttat tattagcaat agagatgcga gcgttcatac
     5941 tctctttagc taaaaacagc aatggtttca actcaggtat ggatagcatc tcctcgttta
     6001 aatcgacagg gtcattaatc tcatctaaaa ctacaggttg aaatttgtca gcaatgatct
     6061 gttcaaaatt tttttctaaa aaatcagcat ctaagttttt cggtacatca ctttcagatg
     6121 tgattttttc agcttcataa acataatttt ctttgtcact ttgggattcg ggtttttcat
     6181 caatttttgg ttgttggtag gtttctgttt gtatttgtgt ttcttctatg cttacttctt
     6241 ggttttctgc ttcggttggt ttgttatcat ccacaaaaag agcttctttt tcatctggta
     6301 cattttctgc tacggcttca aattcaactt cttcagaaga gttgaaggtc tcagcaattc
     6361 gttctaatcc attagtagct acttctaatt gctctactgc gctatagaag tctgtagctt
     6421 cttcaacttc tgattcaaac acattaggat catccggaat aactaataat tgaccactac
     6481 tatccataaa acggacacct gctaatagct tctctttaaa tttttctggt agatcagcta
     6541 cataattcaa acttgacttc aatagaacga catacttctc agaatcgaga atagcatcat
     6601 cctcatctat aactccctca ctgttgagct tttgtacatc ttcaattacc atataatttt
     6661 tctttggtaa atctttggtc agcaacgcat cgaattcttt ccataccttt gctttagctt
     6721 tttttgcctt cgcttctttt tcttcttttt tgtacacttc gagtaattgt ggcttcaaag
     6781 agagtgcaac tacaggaact gtttctgcca cataattaat agattcggta atatctttga
     6841 attttgccat tattgtaaaa ctcctttttt cagtcaatag ttcttttctg tttatctagc
     6901 atatatacat atgctttttc gaatttaata agcgtttcaa gtctggcttt ttcaatcttt
     6961 ttactcccat ttcgataatc atagaactgg ttaacattca tattcattat ctttgccagt
     7021 tcggtacctt tcagactgct cattaatact tcgttggcgt gagctaacat tttttctcta
     7081 tcaaaaacca tcttacattt actttcttcc ctcacttcta aactacgctt ggagcgtagt
     7141 tttttggtaa aaagtaaatt gaacaaatcg gcatattatc caaatttgtt caattcactt
     7201 ttaacctaac agttcatttt ttggaaattt tgaaactaga tagataccaa caactggaat
     7261 aagtgctgat atgatattag ctttttggtt caaatcatta cctgttttag tacccattgt
     7321 gtaaagcata acggttgtaa ttacaggaat aataaagatt gcaataatcg ccccaacata
     7381 ttttaatcca tctccccatt ttgcatcacc aaaaaatttc attgcagtga atcctggttt
     7441 caacgaaaaa acgaatgcca agaaatacaa aacaacttgt actaaagttt tcatatcagg
     7501 cgtcattccg ctaaccaaat ttttaataga atcgaacata taaaaacctc ctttttaata
     7561 tttttaataa gttaattata tcatcgacaa atcttatttg ttcacttgtc aatgatctaa
     7621 ctaagaaaaa gacgtctcga tatttaca
//
LOCUS       DAZKOB010000120         7633 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000120 DAZKOB010000000
VERSION     DAZKOB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.122"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..737)
                     /locus_tag="ACG8M6_002549"
     CDS             complement(<1..737)
                     /locus_tag="ACG8M6_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0458162.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGA"
     gene            complement(857..1504)
                     /locus_tag="ACG8M6_002550"
     CDS             complement(857..1504)
                     /locus_tag="ACG8M6_002550"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0458163.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1588..3015)
                     /gene="uxaC"
                     /locus_tag="ACG8M6_002551"
     CDS             complement(1588..3015)
                     /gene="uxaC"
                     /locus_tag="ACG8M6_002551"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0458164.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3089..4168)
                     /gene="uxuA"
                     /locus_tag="ACG8M6_002552"
     CDS             complement(3089..4168)
                     /gene="uxuA"
                     /locus_tag="ACG8M6_002552"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0458165.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4171..5802)
                     /locus_tag="ACG8M6_002553"
     CDS             complement(4171..5802)
                     /locus_tag="ACG8M6_002553"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0458166.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6106..6777
                     /locus_tag="ACG8M6_002554"
     CDS             6106..6777
                     /locus_tag="ACG8M6_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0458167.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(6937..7128)
                     /locus_tag="ACG8M6_002555"
                     /pseudo
     CDS             complement(6937..7128)
                     /locus_tag="ACG8M6_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         2468 a         1495 c         1031 g         2639 t
ORIGIN      
        1 gcaccaatta tagaaagtaa tgccctattt ttaactaacc cttttaaaga ttttaacaat
       61 gaaactttgt attgagattg gtcaattctt actcgttcag tagttagttt ataacatatt
      121 aaataacaaa taagtgcaca tatcgaaaat attcccgcaa caattgtaaa agttgatcca
      181 ccacgaacta tttgagcgcc tgaatcatct cttgtataaa taaataatgg tgttaataca
      241 ccaatgacca aacttgctaa agtagctcca acatttcgga atgtcgatag cattgttctt
      301 tcttctgtaa tagaagtaat agctgatgcc attgatccat aaggaatatt tatagatgtg
      361 taaaaaatac tcccccataa tatatacgta acatacatgt atactacctt aacgctcata
      421 gaagctgaaa tcatagaagt ttgatacatt aaaaaacttg ctaaagcgac tggaccactc
      481 attcttaaaa tccacggttt aaacttaccc tgcttagttg ctttcgatct atcaacaata
      541 cgccccatag ttacatcagt aactgcatcg ataaatcttg ctactaaaaa taatgttcca
      601 acggcagctc cgcttatccc caatactttt gtataaaaga tcattaaaaa agaactagca
      661 aaaatgaaag taaaatcgtt tccaaaatct ccaaacatat atcctatttt gtctcttata
      721 ccaaattttc tttccataca attctctccc ttctgttgtt aagcgttttc attctgatga
      781 aagtacgtac tatactctga agcgtaaaga gtcagtattt attttttaca tttcagagta
      841 ttatttgttt tttctcctaa taattcttta tctcataaaa cttatctata tatttactag
      901 ctactcgact tacttcttca aaatcacctt tttcaacaga agccaataaa ttcccaccta
      961 caccaactgc aacaactcct acttcaaacc aatcttctaa gttctctata cttactccgc
     1021 cagtaggcat tatatttacg tgtggtaatg gagcttgata ggcttttaca atggatggac
     1081 caaaagcaga acctgggaat agtttaatga tatctacgcc agcagtaagt gcctccttta
     1141 tctcagtaat tgtcatacaa cctggtaaat aaggaatttg atataaatta caaatctcag
     1201 ctgtttgtcg atcaaaagat ggactaacga tatattctgc cccagccata attgcgatac
     1261 gagcagtaaa cgcatctaaa accgtaccgg ctccgattat tacatctttt tgatcagcat
     1321 attttttagc aatttctttg attatttctt cagcattagg aactgtaaaa gttagttcaa
     1381 tccctttaat tccacccgca ataattgaat ttattgcttt aatggcctca tccttatttt
     1441 tcccacgtac aaccgcaatg actccagccg cttctaactt acttaaaatt tctacttttt
     1501 tcatatctat ctccttcctt tatggataca tactttgtct agctgtacta acttacaaag
     1561 aataaattta agcttttatt tcaagtatta ttttctactt ccgatttcaa aaaaattaaa
     1621 ataattatac gcattacgat aacaaatatt ctccactatc tgactaagat acatttcatc
     1681 atctggaatt cggcctgctt taacccactc gcttagtaat tgacataata ctcgacgaaa
     1741 atactcatgg cgaggatagc ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt
     1801 cgacaacaaa ctttgttgtg ccatcgtagt caactgttga cgcattcctt cagctgtatc
     1861 attgaaccac caagcacatc ccaattggat tttttgaatc ataccccctt ggaagctttg
     1921 cattgctgtt gctagtggaa gccaatcagt tggatttaat gaatataaaa ttgtttttgg
     1981 taagcaccca ctcttttcag cggtatcgaa caatgctaca acattttgtg tcaaactagc
     2041 ttgatccccc atcgaatcac caccgacatc tactccaatt tgttccttta atatacttga
     2101 attatttctt aaagcattta aatgcatttg catcgtccaa ccatatcttg tatactcaga
     2161 catcaaatgt aattgaatac ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt
     2221 ttttcctgct aaagctttta ttaacagatt gtcgagtatt ttttcatctg ctatttcata
     2281 aaaataagta ttcaaaccgt gatctgctaa tcgacagcca atttgatgaa aatgatccac
     2341 tcgttggctc aatgcttgct tcaacccatt aaagtctttg attggtatag ccgctgtttg
     2401 actaagtttt aaaatatagt tcgaaaagcc tgatgatttg atactccaga ccttatctgg
     2461 acggaaagta ggtaatacct tgaaagaatt atcctgttta gaaagctcag tatgatacca
     2521 caagtcatct gtaggatcat ccgttgtaca gatggctttc acatccatca tctccaacaa
     2581 ttctttcggg cgataggaat ccgtttgaat tttttcgtta gcacgtcgcc aaatttctgg
     2641 tgcattcttc tcagaaattg tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga
     2701 ccattcaaaa ataggattac ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt
     2761 ttcataatca tcacctggac cactgatcaa ctcttcttct ataccattag ctcgcatcaa
     2821 tcgccattta taatgatctc cagctccttt atcatttaac catacctgag tcaaattctt
     2881 gtattgctta ttttctagca aatatgctgg gtctaaatga caatgatagt cgataattgg
     2941 ttggtgtttt gcaaatttat gaaaaagtct tttagctatt tcattctcta ataaaaagtt
     3001 cttatctaaa aacatattta aacactcctt tccttccgtt agtttttaga agagaaaatt
     3061 tttcctctaa ataaactccc ttatataatt atttactcgc ccccttttca agcgcttccc
     3121 aaataccatt taaataaact gcacctagag ctcggtcata taatccataa ccaggtctac
     3181 cggtttctcc ccaaatcatc cgaccatgat ctggacgaat atagccatta aatccattat
     3241 cgtacattgt ttttaatatc ttatacatat ctagagaacc atattctgat ttatgcggcg
     3301 cttcgtaaaa atcgagtcca ttgtcactta aatatttgat attacgaaca tgtgcaaaag
     3361 gaatccgatc ccgttgacaa aattcagata gaatttcata gacatcatta tctggtcgtt
     3421 ctgcaatact tccagtacat aatgtaatac cattagaagg agaatccact acattacaca
     3481 accaatctaa atcgtcacga tttttaacaa tacgaggtaa tccgaaaatg gaatatggtg
     3541 gatcatctgg gtgaactgcc attttaatcc caacttcttc acatgtcggg ataattgctt
     3601 tgagaaaata tgcaaagttc tctcgtaatt tctcttcatc aacttcttta taggcttcta
     3661 ataattgctt aatttgtgat aaccgatgag gttcccaacc cggcaattca aaattattag
     3721 tagaattttc tacttctcta ataatttcct ccggatcagt agggatattt tttgaaatat
     3781 aggctaacgt agaagaccca tcatctaaac gataatccaa atccgatttt acccaatcaa
     3841 atactggcat aaaattataa cagataacct caatcccaaa ttctgccaaa ttgcgaatcg
     3901 tctgtttata attctcaatg tattgatctc gtgttggtaa accaattttg atatcatcat
     3961 gaacattaac actctcaata actttcaagg taagtccatt ggcttcgact tcatctttta
     4021 aagatttaat tcgttctctc ggccaaacct ctccggctgg tatatccatt aatgttccaa
     4081 caattccttt cattgctggg atctgacgaa tttcttgtag tgagattttt tcttcatttg
     4141 ccccatacca tctaaaagtc atttccatag ttactttcct ccaacttctg ttatatattt
     4201 attgattgtt ttacgaactg cttgaggacc tactagcatt tcgcaaaaga ttttttcagt
     4261 tttctttcct accccaactt gatataaatc taaaccaaag atttctttat tttctaaaat
     4321 ttcctgtacc gcacaatgaa tctttgaagg atcttgttca cctaatttca atactgataa
     4381 tttatcctgc aactctacca ataacggatc aggacttggc acaaaatttt ccccttgatc
     4441 atcgactgcc agtaaataac gtaaccaagc agcaatagcg ataggaataa atgttagatt
     4501 ttctgcattt ccgtcttttt cgacatactt tttaattgtt tcaccatagc gaatcgctac
     4561 tttttgagaa gtatctgttg caattcgttg tggtgtatct ggaatatagg gattaggcaa
     4621 acgtttatta attacttcat caataaattg ttttggatca ataatgcctg gattttcaac
     4681 tactggtaaa ccttctaaat aacctattcc tttgattaaa ccaaccaaat catcatcctg
     4741 catctctgct gaaattgatt gatatcctag cagacaacct gttactgcca aagcagtatg
     4801 aagtggattc aagcttgtcg taaccttcat gatgtctgct ttatcaaccg tctctctatc
     4861 tgtcataatc acaccagcct tttccaattt aggccggcca tttggaaaac tatcttcaat
     4921 aactaaataa tgaactacct cagtattagt gaaagcagca atattggtat gtttttttgt
     4981 gtgaatgata tctaaatcct cgagccccat catcaataga tcttccttta cattctctga
     5041 aggatttggc gtaattcgat caatcatact ccaaggaaaa gtcacttttt ctttatcctt
     5101 taaataagca ataaattttt cagaaacata tccttctttt tgccaagctt ctgcaatcgt
     5161 aaaaatacta tcttgaaaac gttgaccatt ctgtgagaaa ttatcagtag aaaccatcgc
     5221 aatcggcaac tctccagcat taaatctgct atataacaag gatgttacaa tactcatcgt
     5281 atgttgagga ttgctaggtc cagatttgat atcagctttg actaccgaaa agaattgccc
     5341 tttagaatct tttaaactgt aacctttttc agtaatagta aacgttgtaa actgtaattt
     5401 aggatcttca aaatatttaa ccactctttg atagtccgct atattcttcg gattgcaata
     5461 taaactatca gccgttactg ccaaaagtct ttttttgagt tcaccatctc cactcataac
     5521 aatttccaaa atgtcattgt tatagtcatg ataagctttt tccactactt cttcatcaaa
     5581 tgtttcacac acaactactc ctgtttctaa ttcattttga tctgctaagc attgagcaat
     5641 ttctgcatga aatcctcgat aaagatttcc tccaccaaaa tgaagccata ctggatgttg
     5701 ctggccttct tgcttaactt ctttgacatc aaaacacggc agtagaatct gcatttcatt
     5761 gaattcttcg atagaatttt gccaattttt aatcgaaacc ataattaacc tcctcaaact
     5821 gatatgttag ttttgataca ttttatattt taaaacccat tgtaaccgtt gtcaatataa
     5881 aaagggaata aacttgcttt tttctaccat atcagaatga ttgtattttt tattcagaaa
     5941 aataaaatac tccttctttt tggactgatt ttatcgaaac ttattttttc tttttttcat
     6001 gtattctttt atttttataa ctagtgtatt acttcaaaat ttgatattat atggcatgaa
     6061 cagaattaaa atatcagtta aaataaaagt taaggagcat tgtgtatgac aattaaaact
     6121 agtctccaaa atcaagctta ccaatctatt cgccgacaaa ttatctatgc tgacttagaa
     6181 cctggaaaaa aagtatcaga aaaaaaatta gaagaaacac tttctattgg tcgtacacca
     6241 atacgtgaag cactgattca actccgacaa caaggtcttg ttgatacaat tcctcaaagc
     6301 ggaaattata tctcattaat tgatttagat tcggccaaaa atgctcgatt tgtcagagaa
     6361 catttggaac gacaaattat gttagagtgt tgtgccaaaa tgaataacca aaaaaaacgg
     6421 attttagaaa ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa tgctcgtagt
     6481 ttttttcata atgataattt attccataaa gcttgttttg aaattgcaga gcgagaagaa
     6541 gtatggcaat ggttagaaaa taacaataca cacttagaac gatttcgatg gctacgagta
     6601 aaaacggaag gattaaagtg ggaaaaaatt atggaacaac attatcaact atttgaggca
     6661 ttaacaaaac aagatccaga agaagctaac tttctaacat cgttgcatct tcatttaatg
     6721 cttaatgagc aaaatattgt tttagctact catccaaaat atttcaaaaa tatataactt
     6781 ttaactcttt ttaaattaaa tggttgttct gatcagaaaa atatcgcttg ttttcttatt
     6841 atgtaatata tttcataaac tattctgcat aacaactatt gtttgtactt tttacattgg
     6901 ataactaatt atgactgctg ataacttttg atattaaact agtatctcca atacaaataa
     6961 aaaatagata tcttgttcat caaaattagt tcgtggatac ttaccttcat ttacatcatt
     7021 cagcggtttc ccattttctt ttattctatt atttcatttt ctaatagact tggcttcatt
     7081 tcgcaatact ttttttcaat aggcatcaaa ttggtgttgt ttagatattt agttgtagtt
     7141 ttgtgacatg ataagtatgc tcctttttct cttttccact aatacaacga tcaaagggtg
     7201 cttttttcaa aaaactagaa aaatcaaaat aagattcgta ttttgattct atatatcctg
     7261 tttcaactac atcttatatt tcacaataga ataaatagcc ttttgcatag ctgcttaact
     7321 tataagaaat tgcaatttac ttcttcaaag tgaaaacgaa tatgagtaaa ataaaattat
     7381 tcttcttaca gataattaaa aatagcgcgt aacaaaaatc ggcattcaat cacataatta
     7441 ggattgatgc cgattactta tattctacgt atagttttag tattcttatt ttttatcacc
     7501 agtaaccaat tgagaacagc cctaaagcag aatagtcttt tatctcaaaa cgcttaattt
     7561 caggcttagg aatatgaata atcttttgag tatattcttt ctcttttaat cttactgaag
     7621 aaataccact gcc
//
LOCUS       DAZKOB010000121         7559 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000121 DAZKOB010000000
VERSION     DAZKOB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.123"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(96..386)
                     /locus_tag="ACG8M6_002556"
     CDS             complement(96..386)
                     /locus_tag="ACG8M6_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458168.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(504..764)
                     /locus_tag="ACG8M6_002557"
     CDS             complement(504..764)
                     /locus_tag="ACG8M6_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458169.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(901..1314)
                     /locus_tag="ACG8M6_002558"
     CDS             complement(901..1314)
                     /locus_tag="ACG8M6_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0458170.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1770..2255)
                     /locus_tag="ACG8M6_002559"
     CDS             complement(1770..2255)
                     /locus_tag="ACG8M6_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458171.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2394..2609)
                     /locus_tag="ACG8M6_002560"
     CDS             complement(2394..2609)
                     /locus_tag="ACG8M6_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0458172.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2833..3633
                     /locus_tag="ACG8M6_002561"
     CDS             2833..3633
                     /locus_tag="ACG8M6_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0458173.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3652..5520
                     /locus_tag="ACG8M6_002562"
     CDS             3652..5520
                     /locus_tag="ACG8M6_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0458174.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5513..6004
                     /locus_tag="ACG8M6_002563"
     CDS             5513..6004
                     /locus_tag="ACG8M6_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0458175.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5992..6546
                     /locus_tag="ACG8M6_002564"
     CDS             5992..6546
                     /locus_tag="ACG8M6_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0458176.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6564..7559
                     /locus_tag="ACG8M6_002565"
     CDS             6564..7559
                     /locus_tag="ACG8M6_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0458177.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2690 a         1211 c         1435 g         2223 t
ORIGIN      
        1 atttgagatc tttttatact tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg
       61 aagttgggac aaacgagtta atacatttta atacattatt ttttattcaa aaaaaatagc
      121 acaatggcaa agatcgtaag agagatcata agccccaatg aatgactaaa aagaaaagca
      181 gaatcacatg ccatatcatc ccatatttct tcaagaaaat aaataagcgg gaaaaaggga
      241 aggaatagta gaacatcgat ccagcaccat ttttttcttc ttttgaaata acataaaaca
      301 ataaacatga acgtgcaggg aagtaaaaac aaagctttac tcataactaa caaaatacca
      361 gctcctttta tttttttctc ccccacattc aaagagtaac aatggtatgt acttaataat
      421 agaaaaatgg atcaacttgt atgaataaca gctgaaaaga tctgcaccaa ataaaaaact
      481 tccagccaaa actgactgga agtttatttt tttctgaaat attctaacac aaatatgaaa
      541 aagatggccg aagctaataa gccgatttcg tagcaaaaca atgagatacc agagtcgtaa
      601 taataaaatc ctctaatctt atggataaaa tagatcatcg gacaagcaga taagagtaag
      661 tatttgttag gccttttgaa taagtaaaag aaagcactgg tcattatgat aaatggtatc
      721 caaaacaaaa gtttacttaa gacaatcaaa tgatcaactc ccatattttt taccaagaga
      781 gatcataaca tctttatcgt aaaaacaaaa tatttttttg ctcattttga aaatttaaac
      841 atactcgttc ttatccaaat atataaaaat cgcccctcat tcatcgagag gcgataagtt
      901 ctaatttaac tcaatttttt ttgtttcttt tggctgataa gctgttttag gtaataccaa
      961 tttcagcaca ccgtttttaa aggttccagt gattttttct tcatcaacat taggaagata
     1021 gaatgaacgt ttgtaagaga ccgaatgtct ttctttccgt acatagttgc catcttcttt
     1081 atcttctgta tgggactcat gattggcgga gatcgtcaaa gtattatctg aatattcaac
     1141 tgtcgtgtct tctttgtcac agccaggcat atcagcagtt agtttgtatt ctttttcatt
     1201 ttcgactaaa tctacttttg gataacttga aacacctaaa aaatcattga atgctggact
     1261 aaacacgtca ttgaaatcag ggaacatgtc gcgaatgcta ggtacatttg ccatttttga
     1321 tcatcctttc aataagtatt ttttatttat ccctatttac gtaataactc tattttgaaa
     1381 aaaatgcaag tataatggtt agctttgtga atatttcatc taatacgata caaaggctat
     1441 ctttctaatt tgaaaaaggg atcattaaaa taataagaag aaaatatgtc aaaaggtaaa
     1501 aaagtaaatt cttctatcca aatatagaaa aattgaatct agaaaaccgc ccgacaataa
     1561 atttcattaa ctcagttgac cgaaaatgaa gtagatacca aaaatttatc aagagagcaa
     1621 gtagagaagt gagtatattc ataagagaaa tagctgtaac gaattattag gttaatgcaa
     1681 atgcttattt atactccgta taactttctc agaaaaaatg ataccaataa tgacttgcta
     1741 gtactgtaaa tccgtataat atcatctaat caaaatggtg ttccatttgc atctgtcatc
     1801 gtaatcgatc cttcgggtga cactcgataa aaaccaacgc ttccagatcc tccttgggaa
     1861 cggatttgtt tgctggaagc tgtgaacaaa taatctcgat caattttttg ataaaagcta
     1921 ataacaatat cgtcactttg taattcggaa cggtgggcaa taagaaatag aagtgctttg
     1981 tcagagtttt ctaatggaag agaaggtaca gtgctttctt gcatttcaga tgttgaagaa
     2041 atgctaaacg actgaatact tgattggcta tgttgcgaat ctacattacc agtagaagta
     2101 ttagtaatgg aagtagtaga agtactttta gttgaaccaa cctcagttgt tgaaatggct
     2161 gctgaagatt ctttaacaga attctttgtt ttatatccac aggcagaaaa aagtattaca
     2221 gttcccaaca agcctgcgta aatcccttta tgcatgatag ctcctcctaa aatagattat
     2281 ctttattcag tatatcactt aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc
     2341 ccaataaagg gaaggttcat gaagataata acttttttaa taattatagc tttctaatat
     2401 ctttcatcaa cttttctagt aatcattggg aaaaatttca agcagtagat cgcacaaata
     2461 cctacgacga tataaatgat cttcgcaaaa atagttgttg aaccaccagc aatcatagct
     2521 actaaatcaa gttcgaacaa gcctaccaac agccagttca accctcccac aatcaataaa
     2581 gttaatgcaa tagcatctaa agttttcatt agaattctcc tttcttaaat gataaaacaa
     2641 ttatatatca aaaagagaaa cattgcttat ataacgctca ggaattttag caatctatgc
     2701 aaaaagatga aaattttttt tgctcttcca ttagtacaca ttttttagct gaagctgtaa
     2761 atttcatgtc ttaatttttt ttagtgagct tttatactgg atttgtaaga aaaaagaagg
     2821 agaggatcac atatgtcgga ttggttggat attgcaggaa aagtagtaat tgtaactgga
     2881 ggttcctcag gaattggaag aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta
     2941 gcgaattttg atgtaactga atgccgtata cagcatgaaa acctactatc tttaaaagtc
     3001 gatgtgtctt ctaaaacgga catagaagaa gggatatata aagtaatgaa acatttcaag
     3061 acgatcgatg gtctagtcaa taatgcagga ataaatattc cttctcttct aatagataga
     3121 aatcatccaa aaagcaagta tgaattatca gaacaagttt ttgataagat gatcgctgtc
     3181 aatcaaaaaa gtgtatattt gatgagccaa gcagttggaa gaatcttggt gcaaaaggga
     3241 tcaggagtga tcgtgaatct ttcttctgaa tcaggattgg aaggatcaga aggacagagc
     3301 tgttatgctg ctacaaaagc agcgatgaac agttttactc gttcttgggc aaaagagtta
     3361 ggaaagagga atgttcgtgt ggtaggtgtt gcaccaggga tactggaaga aaccggtttg
     3421 agaacacaag aatatgaaga agctctttct tatacgagag gtataagcgt cgaacagctg
     3481 agaaacggtt acagtcgaac aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa
     3541 gtggcggatc tagtctgcta ttatctttca gaaagatcaa gttatatcac tggagtcacg
     3601 actaatattt caggaggtaa aacaagagga taaaggatag gggggacgca cgtgacgcta
     3661 gtaaacagat ggtaccaatt attgaaatta ctagtcactc acaagaaagt ttcaatccag
     3721 gaagtgcaaa aagaaatcaa aaccagtaat caaaccataa aaaaagatat tggggagttg
     3781 aatgaagaaa ttgccggtat tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa
     3841 atttatgatt ttgatttttt tgatgagatc atgcaaggaa aattgaaaac agccagtgat
     3901 ttcaattcgg catccaaaag aatcgcttat ttactaaata ggctgatcga aacagaggat
     3961 ttcttacgta tggatgattt gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag
     4021 gatctaaaga cattaaaaga gatgatcaat gattttgatg taaagattac aggcacacca
     4081 aataaaggac taagaataat aggagatgag ctagaattac gactactttt attttatttt
     4141 gtttctccct acttttcaga gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc
     4201 aaacattttc agaagaagac gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt
     4261 atcggtattt ctttgaatag aatctctagt aactatctgt tgaagaagcc catcaatcgt
     4321 tacagtgggt gttttgaata tatccaagaa ttcaatgaac tgatcaatca gttggaagaa
     4381 atctatgaac taactttgag ccaatatgaa aaagatttta tcagttttcc tttgaacatc
     4441 atcaacacaa gtataacgat gataaggaaa gctaactctg atttgcgctt atatttcgac
     4501 cccatgatga tgcgtattcg acgattagat ctcattgatc tggatgagtc cttcttatat
     4561 caagagatga aacagcacct ctctttattg ataaatcggg taatttttcg ttatcgattg
     4621 actgatttat tttatggtga gatagaaaaa aagtatcctt tttcttacca aatcgctagc
     4681 gaatgcatac aagcattaga agctatcttg caacataaga tctctaaagt agagatcagc
     4741 tacctaacgt tgtatttgga gttagcattt cgatatcaaa aagagcattt ttcctctaaa
     4801 aaagtagcca ttgttcatac gacaggaaaa ggaacagcat taatgatcca aagacaaatc
     4861 aaaaggatat tggggaggga tatacaaata acacatgttt cagaagaaca atatgaaaaa
     4921 gtagatctaa acaaatattt cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa
     4981 gagacaccgg tgatccattt gactaatctt tttgatgact cttggttaag ggcacaatgg
     5041 caaaaagcaa aaaaaaagcg attagccggg tttcattcta ctgttttttt ctatcaaata
     5101 tggaaagaaa aaaagacgta tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga
     5161 ggctattatg tggatgaaga atttgaaaaa aggatcttga aaagagaata tcagcaaagc
     5221 acatttgtta acaaagaatt tgcttttcct cacacaacca accagctatc tcaacacatt
     5281 gttttatcag tagggctaat aactgatcaa ggtttagcag aaacaggagg aaatggagtt
     5341 atttttttat ttgccatacc tgaagaaatg acggctgaag ttgagaatga attatttgag
     5401 ctatacgata tcctatttga actcataaaa atgggaatag atgagtttat tgaattagca
     5461 ccgacacaag aatcattttt aaaactaatc aaaagtaaag gagagagact tcatgaatga
     5521 tatgtatcta ttcatagttt tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg
     5581 gaagcaacta aagcatttca atcaaacata tagcgaactg cgaagaaaag gaaaagtagc
     5641 gattggtaaa cgacctggga aaatccgttc aggcacgatt gttatgctag ctatagacga
     5701 tcatgatcaa gttttagaag tcagaaaaat gcaaggggtt acatctttgg caaaatttca
     5761 ttcattacct gtctatgcgg gtgaagatat ccattacatc gatcgttacc atccctcagt
     5821 tcgagcggaa gatcgtttga cacaagcagc tttagaaaat gccagagaag tatacttacg
     5881 aatagaggca ggaaatgaaa aaagtgaaca accttcattg ccttttattg ctatgctcaa
     5941 taattttcgg atatggaaaa gtacgatcaa agaaaaaagg agtgacaaac gatggatatc
     6001 ataacaaaat ttgctgaggg atttatgagg ctgttccaaa caggtgcaga aacattcata
     6061 agctggatga caggaatcgt accagttgtc ttgatgctga tggttgcaat gaatacgctg
     6121 attgcttttt taggtgaaga ccgagtaacg aaagtagcca aattatcagc gaaaaatccg
     6181 cttactcgtt accttgtgtt accgttctta tctgctttta tgttgggaaa tcctatgtca
     6241 tttacaatgg ctcgtttcct tccagaatac tacaaaccaa gctattatgc tgctcaagct
     6301 caattttgtc atacgagcaa tggtgtattc ccccatatta atcctggaga attgtttgta
     6361 tggatgggga ttgctcaagg gatcgaaacc ttagggctaa attcgatgga gctggctatc
     6421 cgatacttgc ttgtgggact gctgatgaac tttattggtg gttggataac tgattttact
     6481 actggatttg tatgcagaca gcaaggaatc gttcttagca aaaaagtaga gctttcagtt
     6541 gattagagaa aggagagttt taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg
     6601 ggtatggagg tcctttgatt attaggccga caaaagataa acacaagttc atctatgtca
     6661 caggtggagg tgagaggcca atgatcgtag ataaaataga agaactgaca ggtatggagt
     6721 ctatcaatgg gttcaaaaca tcaatacctg atagtgaaat cgcgcttgca atcgttgact
     6781 gtggagggac tcttagatgt ggaatttatc ctaaaaaagg gataccaacc atcaatatcg
     6841 tttccacagg aaaaagtggc ccattagcac aatatatcac tgaagatatt tatgtttcag
     6901 cagtagggtt agaacaaata cagccactaa tggaaactga tgataacaaa gaaacagtaa
     6961 atactgcatc tccagataaa aagtacaaat atgatacttc taaaaagata accgagcaaa
     7021 aagcagagca gcaaaactgg atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta
     7081 cattcaatca atcagcaaga gaagcagtag atacgatgat ccggacagtg atccctttta
     7141 tggctttcgt ttcccttttg attggtgtga tacaaggttc tgggatcggt aatgtatttg
     7201 ctaacattat ggcaccttta gcaggaaatg cagttggatt aacaatcatt ggctttattt
     7261 gttcactgcc atttttatct cctttgcttg gtccaggggc agttattagc caagtgattg
     7321 gaacattgat cggtgtagag attgggaaag gaaatattcc tccccaatta gcattaccgg
     7381 cactatttgc tatcaatact caaaatgcat gtgattttat tccagtcggt ctaggtttgg
     7441 aagaagcaaa agctgagaca gtagaagtag gtgtaccatc agttctttat tcaagatttc
     7501 ttaacggcat cccacgagtt atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DAZKOB010000122         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000122 DAZKOB010000000
VERSION     DAZKOB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.124"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG8M6_002566"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG8M6_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0458178.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG8M6_002567"
     CDS             1483..1935
                     /locus_tag="ACG8M6_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458179.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG8M6_002568"
     CDS             1949..2638
                     /locus_tag="ACG8M6_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458180.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG8M6_002569"
     CDS             2763..5114
                     /locus_tag="ACG8M6_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0458181.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG8M6_002570"
     CDS             5194..5589
                     /locus_tag="ACG8M6_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458182.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG8M6_002571"
     CDS             5914..6531
                     /locus_tag="ACG8M6_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0458183.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG8M6_002572"
     CDS             6572..6871
                     /locus_tag="ACG8M6_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0458184.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG8M6_002573"
     CDS             6905..7072
                     /locus_tag="ACG8M6_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0458185.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG8M6_002574"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG8M6_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0458186.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKOB010000123         7374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000123 DAZKOB010000000
VERSION     DAZKOB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.125"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..1340)
                     /locus_tag="ACG8M6_002575"
     CDS             complement(66..1340)
                     /locus_tag="ACG8M6_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0458187.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRDTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1519..2166)
                     /locus_tag="ACG8M6_002576"
     CDS             complement(1519..2166)
                     /locus_tag="ACG8M6_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0458188.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2174..2860)
                     /locus_tag="ACG8M6_002577"
     CDS             complement(2174..2860)
                     /locus_tag="ACG8M6_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0458189.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2866..3939)
                     /locus_tag="ACG8M6_002578"
     CDS             complement(2866..3939)
                     /locus_tag="ACG8M6_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0458190.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4161..4835
                     /locus_tag="ACG8M6_002579"
     CDS             4161..4835
                     /locus_tag="ACG8M6_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0458191.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4840..6180
                     /locus_tag="ACG8M6_002580"
     CDS             4840..6180
                     /locus_tag="ACG8M6_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0458192.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6329..7315)
                     /locus_tag="ACG8M6_002581"
     CDS             complement(6329..7315)
                     /locus_tag="ACG8M6_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0458193.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2205 a         1408 c         1347 g         2414 t
ORIGIN      
        1 ggcgaagaca aatttgaata aaacttagtc cgatatgcgg cgcgttctgt gcgccgcata
       61 tttttttagt tttcaaagat cagaccttgt tgtaaataga tccgtgcccg aaagttataa
      121 aagttgcgat aaccaaacgc aattcgttta atcagcttga tcttgttatt cagcccctca
      181 gtgacgccgt ttgagtgggg caccttaaag gcatttgaaa tacccgattg gtagcgatgg
      241 aagatcgtga atttcttttt aaacgactga ggtaacgtag atgaaattgt tttaagggtt
      301 tcttgaaagc cttccgtatc tcgtttaacg aaataatagc gtaataattg acaaaaatga
      361 tacgcagttc gcaattcttc gttgtagctt aacagctcat caacgatttc tgtttgacta
      421 attggtcgtt taaacagtcg atgataatgc cgtttgagtg gctccaatgt cgtggaatct
      481 ttgagtaaga gtttccaata tcgcttgagc cgacggtact tcttctgatt ttcagagtgt
      541 gaggttcgaa aacgattcat gatctggaca cgtaattgat tgaagctgcg attgatgtgt
      601 tggacaatat gaaagcggtc agttacgatc tccgcatttg gaaagactgt tttaagcaac
      661 tgacaatagc tggcattcat gtccatcact aggtacttga cctttaaacg ggcctttcta
      721 ggaaagcgca ggaagtattg agttagtttg taaagtctgc gatcttcaag gatcgtaaat
      781 agctgattgg ttgtcccatc aacacaaata aaactcatgg aaccagaaac agacttcatt
      841 gatttgaact catccataca caacaccgtc ggaaggaagt tcatacgtgg ctggtaatgc
      901 ttcgttgctt cacgcaagat ccgtgtcacc gtcccatccg agacgaagta cttcttagcg
      961 attagtttac gtgattgatt ttcgtacagt tccatgagaa tcgcctgctt cagttccttt
     1021 gttagttgat gatgttcatc aactaagtcc gttttcgcag aaaaggtccg tccacaatca
     1081 tggcagagaa aacgggtacg ttttaattct aaaatagtca acttttcttt gactttcaac
     1141 atctgagtgc gtgtcgtata ggaaccgttg cggataatac gaatcgatcc acagtgttca
     1201 caatgggaag gtgagtcatc caatctccct ttaataaact gcgcttgaac gcctttatat
     1261 tctttttcaa tcagccattc ttcatcaaaa aataaatgtt tatctgttaa tccgagtaat
     1321 tttctagtat gattatccat tgagagactc ctttggtagg gtgacgtttt ggtcgactaa
     1381 attttaccaa aagtgagtct ctttttgagt tgaaacgaag aaaaaagtgt tagtagaaaa
     1441 tcgctttcga ttttccacca acacaaaata ttatagaacc ataaaaattt caggacaaat
     1501 acacatagct gtttcttttt attgtattcc ttcagcaatc caacgagatt tatagcgaaa
     1561 gtcttttaac aactcttctt ttgtccaatt atttgcaatg agatccgtca aagtgttcat
     1621 tacgagggtc atggaaaagt ataggaaact aactgcttct tccgacgatt gaatggagaa
     1681 atgattgatt cttaataatt ccagccattt ttcgttcatt ggagaatcag ccgatagttc
     1741 tctacgtttg gttgtctgct gatcatttcg ctgggttaac aagtataacc tttgccaata
     1801 gatactttcg tgatctaagt cgcagcacca ctctaaatat ttttcgatcc ccaaaaagaa
     1861 atgttgtttg ttttcgctaa tgaattttaa gatatcttga tggactatgt gagaactgct
     1921 tttgatcgta tacaaataag catcttccaa atcttgaaaa tatttataaa aagcaccgcg
     1981 cgacatcccc atcttgtcaa cgatttctga gactttgact tggctgatgt gctgtgagca
     2041 aaatatctcc agtaataggt catcgattct ctgtttcttt gcgttcgata aattgaaaaa
     2101 agtttctttt ggcatccaca tctctccttt atctctgatt atacgtggat agcccttcat
     2161 tgtcatgaag gattcattcc ttctttttct cttcttctgt caagtaccca tcttccattc
     2221 gataaacgcg atcactccat tgaatcatac ggacatcatg agtaaccata atagtcgctt
     2281 tttgtttctc atgagcttct ttaactagta atttgacgac ttcatatgca tgatctgtat
     2341 ccaaactagc tgttggttcg tctgctaaga tcaagcttgg ctcattatat aaagcgcgag
     2401 caattgccac tctctgtctt tctccaccag aaaggtcttt aggataacta tttttaagtg
     2461 actggatatc taaagattgc agcaaggaat cgatttttgt tgaatcttct ttttttgaag
     2521 taacttggtc aactagttca aattgttcct ttaccgttaa aaaaggaatc aaattagagc
     2581 tttggagaat aaatcctatt tctttaaagc gtaaatctgc tcgttttttt tctggaagct
     2641 cagttagtga gtgaccgttg atcactattt ctccggaagt cggtgtttgc aatcctcctg
     2701 aaatggtaag gaaagtactt ttacctgaac cagaaggacc aatgatgctg acgaattctc
     2761 cttcctttac ataaaaatca attgctttca aagctgagac ctttgtatga ccagaaccaa
     2821 atgttttggt aacttttttc atcgttaata tttttttcat ttttattaac ctcctattgc
     2881 tgtaattggg tctactttgg ttatcgtacg tatcgaaaac aatccgccta gcattgcgac
     2941 aatgatcaaa atcccactat atagcaacca ttgtgaccaa aaaacagcaa aaggcatcgc
     3001 gcttggtaaa atcagactag tcaaataagc aaacaaaaga gcaagcagaa cgcctaccac
     3061 cccaacaata aaggactgtg ctactaaaga tttagcgatg aagaaatttt tgatcccttg
     3121 tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc atcacgtaca tgaaaatacc
     3181 gaccacggca gcaaccacta agaacaaaaa gtatatcatc gcatttaagg tcatattttg
     3241 ggcagaatag ccaggaatac tttcaatgaa tgtcccaata gttaataact gtagaccctt
     3301 cgatgctttt tctttcgaaa ttttagctga atcctttatt acgatcccat tgatcggctg
     3361 ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa gcagtccacg tatctaggga
     3421 cgcatagatg acgggagaaa ctgtgtaata agtttcagga aagataccga caatcgtcaa
     3481 aggatcgtcg taactgccaa ttttgatcgt atctccaatt ttatagcctg aatcagccag
     3541 attttgagag atgatgattt cattctttgt tttaaattca tgccctttcg tgagttttgg
     3601 taataaaaat gcttctttcg ttgtgccgaa aactgtaaca ttttctttgt tttttccttt
     3661 gattgctccg ctgtacaatc cgattggtgc tttctctcct ccttcaatgt atttcagatc
     3721 tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca tcctccgaca aaacaatctc
     3781 ttgtgcgtcc caatcatcga ttgctttttt aaactcttct gaaagtccat tcgctagtcc
     3841 tgacaacatg aatacgacat acgcaatcaa gaacataata ccgacgatca agccatatcg
     3901 taatttagaa tagcgaattt ctttgatagc tagaaacatt tgtttttcct ccctatgata
     3961 gattgatagt gacaaaatgt cacttgttga atagtgtaac tctaaagtga caatttgtca
     4021 cttgtgttgt ttttttctta tcaataaatt gtctaagaca tctttcgata aaaccttaac
     4081 atgagtcata aaaaatttat ttaagcctta tttatgcttt ccatgataaa ctaaagtgac
     4141 caaatcaatg aggtgggaaa atgattaaac tattagtagt agaagacgat aaagtattat
     4201 ccaataatat aaaagaaata ctcgctgcaa tcggcgagat aacacaagtt tatgatggta
     4261 gtgaaggact gttcgagttg gaaagtggag tttatgatct tgcaatcttg gatttgatga
     4321 tgccggaaat gaatggatat gaagtgttag caaaaatacg gaaagaaaaa atacagactc
     4381 cagtattgat tttgactgca aaagatagtt tggaagataa gattgaagga tttgaacgag
     4441 gtgcagatga ttatctaaca aaacctttct atcgagaaga actgatcatg cgcgtgaaag
     4501 cattattgaa acgctcgcta ggtctctttg atgaaaatgt tctgaagtat aaggatatca
     4561 gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca agttttgccc atacaaggaa
     4621 aagagtttga attgctcgtt tattttttac aaaataaggg aatcattttg acaaaagagc
     4681 agatatttga ccggatctgg ggatttgatt ctgatacgac gattacagtt attgaagttt
     4741 atatgagcaa cttacgaaaa catctgcgaa gtactggatt ggataaagaa atcaaaacct
     4801 tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa tgaatggaga tattacacga
     4861 aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat ttgcttttat cttttttctt
     4921 ttgggattca ttactttaca gatattaaac caatctgcat acagagaaac agatctgtca
     4981 ttagagaaaa taaattttga ttctcgaaca gtacaattag agatacaaag atatcagcaa
     5041 ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca gaacaaacat ggaacagcca
     5101 ttaatggaac catcaaatga ccgttttaac actcaagtga tcttgtggtc taaaaatggt
     5161 gagatattga ataaagcagc tattggtggg cgtttcaacc aaatcaaaga attaaaaatg
     5221 gatacgaaaa atctgaatac catccaaaat attgaattaa atgaagacag tggctcatct
     5281 gccctctctt tccgttcgat tacgaaaaaa gcggagacga atcaagatgg cgttgcctac
     5341 atccaaattt tagaaaatac aaatcaaatc aagaattcat tagcgacttt tcgcacgatt
     5401 atgattttgt gtatgattgt tttttggatt ctctctattg gtatcagtta ttacttgtct
     5461 agtctaagca tgcgccctat tattctttct tggagaagac aaaaagaatt tgttgagaat
     5521 gcttctcatg aattgcgcac accgttaacg attattcaaa atagtttgga acatcttttt
     5581 accaagccta atcatactat tattgaagaa tccgaaagca tcgctcaagc attgtcagag
     5641 acgaggcgcc tcactggatt gacttcggat ttgctgacaa tcgctcggaa tgattctgat
     5701 cagcagctgc tttcaaagca aatgatcaat acacaagaat atatcgaaca acttgttaaa
     5761 ccatttcaag agatggccat catagatgga aaagagttta ttctccataa cgatgcaaca
     5821 ttagaagtac caattgatga aaaaaaaatc catcaagttt tggttatttt gctggataat
     5881 gcattaaaat atacgaatcc agaagataag atcattttgg attcatctgt taaccataaa
     5941 gaatggctga ttcacgtaaa gaatactggc ccttctatca gtgaagaaga tcaagcaagg
     6001 atttttgatc gattttatcg ggaagatcaa tctcgggcga aagagactgg cggatacggg
     6061 cttggcttgg caattgcaaa acaggtaatc gaacagcatc atggacagat ttctgtatcc
     6121 aattatcttc cacaaggagt cgattttaaa gtccaattgc cgttgaagcc taaaaaataa
     6181 tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca cagccctttt ttgttgcgaa
     6241 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcttt aagctgacca
     6301 cttctgtcac aacctcttta gcttacgctt acgatttttt attgcggtct tcaatgtgta
     6361 catcaccgct tgtgtcaata tccaattctt ctcggctaac atcttctgtc actttttcaa
     6421 catcttcatg ttcttcttta tgaatcccaa cttcttcacg aataactgta tgtttgctta
     6481 catctacttg ctcttctttg attgggatag taatcgtgtc atcttccaat ttttcgtctg
     6541 ttccctgtga agattgatct gttactggtt tgcgttcgat gacgatttct tcacgtttga
     6601 ccggtacttc taccgtttca gtatcgttga ccacatgttt gtggatgtcg acttctccag
     6661 ttgtcacatc atgtgtattt acatctaatt tttcttcttt tagttgaatt gtatcatcat
     6721 ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt acccattgta tcagtctcgc
     6781 caggtacgtc catcggatca gttcctggtg tcatattcaa tggatccgtt gtattttgaa
     6841 agcctgtcgt gccacctacg tccattggat ctgttccagt gccagtttct aatccagtcc
     6901 ccattgtgct tgctgggcta acagtatctt gtgtatccat tgtatcttct gttgtttctg
     6961 gctgacgata gccttttact gtgatgacaa attttccatc agaaatatct cctctatatt
     7021 gatataaagg atcattttct ggtgtagccg tctcagaatc ataggtatca aaagaaaaag
     7081 tatcttttat tttttcccat aaagaacggt cttcttcacc atccatccga ttttcatctg
     7141 tttcaacatc tataccagac aatccgtcaa atcctagtct agatgctgct tcatcagtag
     7201 tataaataac gatatcatct cgttcatacc cttcgttacg caaccgttca attactgata
     7261 atgcttcttc cctcgtacta tagcttccga tgaccgttaa atttttctct tccatgtaaa
     7321 ttcctcctag caaatagatt tatgattggt atattgttac ttataaattt attc
//
LOCUS       DAZKOB010000124         7217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000124 DAZKOB010000000
VERSION     DAZKOB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.126"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(230..907)
                     /gene="phoU"
                     /locus_tag="ACG8M6_002582"
     CDS             complement(230..907)
                     /gene="phoU"
                     /locus_tag="ACG8M6_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0458194.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(920..1678)
                     /gene="pstB"
                     /locus_tag="ACG8M6_002583"
     CDS             complement(920..1678)
                     /gene="pstB"
                     /locus_tag="ACG8M6_002583"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0458195.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1694..2500)
                     /gene="pstB"
                     /locus_tag="ACG8M6_002584"
     CDS             complement(1694..2500)
                     /gene="pstB"
                     /locus_tag="ACG8M6_002584"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0458196.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2515..3399)
                     /gene="pstA"
                     /locus_tag="ACG8M6_002585"
     CDS             complement(2515..3399)
                     /gene="pstA"
                     /locus_tag="ACG8M6_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0458197.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3399..4319)
                     /gene="pstC"
                     /locus_tag="ACG8M6_002586"
     CDS             complement(3399..4319)
                     /gene="pstC"
                     /locus_tag="ACG8M6_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0458198.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4434..5291)
                     /locus_tag="ACG8M6_002587"
     CDS             complement(4434..5291)
                     /locus_tag="ACG8M6_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0458199.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5628..7076)
                     /gene="cls"
                     /locus_tag="ACG8M6_002588"
     CDS             complement(5628..7076)
                     /gene="cls"
                     /locus_tag="ACG8M6_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0458200.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 tttgcattct ttatcctttc tatgaaatga tgaaaagggt ttgtgacatg ttttgtcaca
       61 aaccctttcc cgaataaacg gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa
      121 acgaaaaata tgaggagcta ttttttgttt gactgttctt attacttgat gtagaacagc
      181 tttttcacaa cctcttttca taaacaaacg gcataatcac agcttcagtt tagaaaatct
      241 cattgtggtt cgtatttaac tcagtgattt tccctgtagc caagtagacg atccattcac
      301 agatgtttgt cacatagtct ccgatccgtt ctagataact ggcaacattc aagtagtctg
      361 ttccactgat gactgtctca ggattttcct gcatattttt gattgttgca ttatagattc
      421 gattgaaata gtcattgact ctttgatcca tattggcgat cgtacgtgcg tctttttcgt
      481 ctgttttgac ataagcaact aggacgttgt ccaccatttt tttcacataa tcggacatat
      541 cggagatctc tttttcgatt tccggtattc tcgtctgccc ttttacgcga atcgtggatt
      601 tagcaacaga caccgcatga tcggccatac gttctaaatc cgagctagct ttcatgaccg
      661 taatgatcat acgcaaatca gttgtaactg gctgttgcaa tgcgatcatt tcaaaacttt
      721 tcttctccaa gcgtgtttcc atgttattga tatttatatc gttttcgata acttcttggg
      781 ctaagatttt atcgtgtttg atgtatgcac gaactgactt gtgcacggcg ctgctcacca
      841 tcatccccat ttcgtagaac tgattatgga gattcagcaa ttcttcttca aattgtgtac
      901 gtagcattcc gtttcctcct tatccaaatc gacctgaaat ataatcttct gtctcttgtt
      961 tttctggatt catgaatatc ttcttcgtat cgttgtattc gatcaagtga ccatctaaaa
     1021 agaatgccgt cttatctgaa atacgtgaag cttgagacat attatgggtc acgatgatca
     1081 tcgtataccg ctctcttaat tctaacagca tactttcgat tttcccagta gaaacaggat
     1141 caagcgcact tgttggttca tccaataaga tgatttccgg attaactgcc aaaacacgtg
     1201 cgatacatac acgctgctgc tgcccgccgg ataaagataa agcactttta tgcaatttgt
     1261 ctttcacatc atcccaaaca gacgctgcct tcaggctttc ttcaactact tgatcaagga
     1321 cttttttatc tttttcccct tttaatttca atccatagat gacattttca tagatagaaa
     1381 aaggaaatgg gtttggctgc tggaatacca tgccgatttc ttttcttagt tcaacaacat
     1441 ctgtttttgg accatagatg tctttgtctt tatataaaac acttccggtg atcgttacat
     1501 tcggaatcag gtcattcatc cggttcaaag aacgcagata agtggatttt ccgcaaccag
     1561 aaggaccgat catcgcagtg atttcccctt gattgaaact tagatcgatc ccttttagtg
     1621 cttctttctt tccataatac agatgaagat ctttagatga aataatttct tttgtcatgt
     1681 tgaggcctcc gtcttaacca aaatgaccgg acacataatc ttcggttgcc tgaatttttg
     1741 gtctggtaaa tatcttgtta gttttgtcat attccattac ttttcctaga tagaaaaagg
     1801 cagtatagtc gctgatacga gctgcttgtt gcatgttatg cgtcacgatg atgatcgtat
     1861 agtcttcttt caagtttaca agtgtttctt caaccgttcc tgtcgaaata gggtccaaag
     1921 cacttgccgg ctcgtctagc agcaagatat caggtttcat cgcgatcgca cgagcaatgc
     1981 ataaacgttg ctgttgcccg ccagataaag ccaaggcact tttgtccaag ctgtctttta
     2041 cttggtccca caaggcagct tgtttcaagc ttgtttcaac gatttcatct aatttctttt
     2101 tgtttttttc gccgtgctgt ttcaaagcaa atgtaatatt gtcataaatc gacttgctga
     2161 acggattagg acgttgaaaa accatgccaa tccgtttgcg catctcataa acatccactt
     2221 gaggcgcatt aatatctacg ccttgataca tgattttccc agtgattttc gtattagcga
     2281 tcccatcatt cattcgattc aacgaacgaa gatacgtaga ttttccgcag ccggaaggac
     2341 caatcagtgc agtgatctta tttttttcaa attccagatc cactcctttg attgcttcat
     2401 tgtcgccata ccagacatgt atatcatccg tatatagtgc tacttctttc tcttttggca
     2461 tggtgatgac atttgtttca ttccagttat attttttcat tttgcttctt ctccttaggc
     2521 tgaagtcatt tttcggtata atcgtttgcc aatcgcacga gcgccgaagt tgaacagtaa
     2581 aacagcaatg atcaacacgg cagaagcgcc agccgaaaca gaaacaccat caggcatcgt
     2641 gccttctgta ttgatcttcc aaatatggac agctaatgtt tcagcttgac ggaaaatgct
     2701 gatcgggctt gaaacactta atggattcca gttcgtaaaa tctaacgcag gtgcactttg
     2761 tccagccgta tagatcaatg cagctgcttc accaaaaatt cttccagaac ttaggatcac
     2821 accagtcaaa atccctggtg tcgcttctgg aaccaccact ttcatcactg tttcccagcg
     2881 tgacaaacct aaggccaatc ctgcttctcg ttgtgtgtaa tggaccgctt gtaaagactc
     2941 ctcaactgtt ctagtcaaaa gaggcaagtt gaatacagtc aaagccaatg caccagaaat
     3001 aatcgagaaa ccatactgga attgaataac gaaaaccaag aaaccgaata aaccgacaac
     3061 gacagaaggc aacgagctca agatttcaat cgacgtgcga atcacatctg ttagccagtt
     3121 ttttccagca tattctgaaa gatagatacc cgcacctaaa gaaataggaa aactgatgat
     3181 catcgtgatc aaaagcaaat aaatcgagtt aaatagctgg atcccgattc caccaccttc
     3241 ttggtaggct ttggaaggtt cggtcaagaa ggaccatgaa atgtgcggta ttccccgaac
     3301 taagatataa agtagtaaag ctgctaaaat caatacgatg atccccgaaa ctatatataa
     3361 aacacctgta gcaattttat ctgagcgttt tgcgttcatt atttcagctc tcctttcttc
     3421 ccaatcatac ggatcacgat attaaagaat aatgacatca atagcaagat cagcgctaat
     3481 gaccataaca cattattttc aactgttccc atgattgtat ttccgattcc cattgttaag
     3541 atggacgtca atgtagaagc aggagtagta agtcctgttg gcatcaatgc tgcattaccg
     3601 atgaccattt ggatagctaa ggcttcacca aacgcacgag ccataccaaa aacgactgct
     3661 gttaaaatcc cgggcacggc tgctcgtagc acgactttgt agattgtttg ccatcttgtg
     3721 gcaccaagcg ctaatgaagc ttcccgatag tgccgtggta ctgcctttaa tgcatcaaca
     3781 gtcatcgttg tgacagtcgg caagatcata acgaacaaca caaatgtacc agctaagata
     3841 ccaaaccctg tcccgccaaa aatacttctg acaaaaggca cgataactga taaaccgata
     3901 aaaccataaa cgacagaagg aatacctaca agcagctcga tgaccggctg taagatcttc
     3961 gttccgtttt taggagagat ctccgtcatg aatactgctg caccaatggc aaatggcgtg
     4021 gcaacgatag cagataaaaa ggtcacaata aacgaacctg tgatcatagg caatgcccct
     4081 accattggtt tcccatttgc tccgatttgg cttggattcc aatctgtacc aaacagaaaa
     4141 tcaaatacat tgatcttatc agtaaaaaag gtggccaatc ctttactagc tacaaaataa
     4201 aaaatagata atacaaccaa cacgatcagc gcgatacata aaaaactgat gaattttcca
     4261 cgctgttcca tttttgaacg ttttgatttt gttaataata cttttttcac atcttccaca
     4321 gatgactccc ccttaatcag gtttaacata ctcactcagt gtacaaattg aacatcaatt
     4381 tggttttaat aacatgtaaa agtttagcta agtttgtaaa ttttgtgtaa agattactct
     4441 ttaacgatat ttccttgcca atcacgttcg actttcatat gagaaacagg gatataacct
     4501 agttctccaa cgatgttatc ttgtacttca tcagataaga tatagtctaa aaattctttt
     4561 gtcagttttt ttggatgatc tcttgtgtac atatgctcat atgcccaaat cgtccatttg
     4621 ttcgttgtca cattttcatc tgtcggttct acaccgttaa cattcaacgt atctacatca
     4681 tcggaaacat aagaaaaagc aacatagctg attgcaccgg gtgtgtcggc aacgatctgg
     4741 cgcaccattc cgcttgaatc ttgttcttgt gcctgaacag ctgtttctcc gtccaatacc
     4801 catttttcaa acgtgctacg ggttccgctt cctgaagcgc ggttcaatat aactactggc
     4861 tgatcttttc caccgacttc actccagttt ttgatctcgc ctgtaaatag ttttttcagg
     4921 ttttccatac tgatgtcttt tacgccaata tccttgttca caataggagt gatcccaaca
     4981 actgctactc gatgatcgac taatgctgag gcatcgatcc ccgatttttc ttccgcaaac
     5041 aaatcagagt ttccgatgtc gactgctcct gactgtactt gagacaaacc agttccgctt
     5101 ccgccccctt gtacattgat gaattttcca ggattttctg tctggtaagt ttcagcaact
     5161 gtttctacta aaggctgtaa agcagaagag ccaacagccg taatggattc tcctcgatca
     5221 atccactttg aacagcctga taaaagaatg gctaatccta ttattggtaa gaataattta
     5281 agttttttca ttagaaatct cctttaatcc taaagtatgt ttattttatc attttcatta
     5341 cttgatgaaa acgaatcaaa atcattaaat aacaaaataa attctcataa ttgtaaaaaa
     5401 aactatctca ttaaaagttt ctatgctata ctggctttga aatgtgtctc ttttaatagc
     5461 atatattagc ttatttcttg ctttccccaa aaaacaagag ataccccaaa gttaatttcg
     5521 ctcatgcgag acgctttctc catttctgac gtactctcag aaatggcccc gttagaaaag
     5581 gacacgagat ctccatctgt tgatggttct catgtccttt ttatgtttta aaggagcggc
     5641 gaaatcaatc ttgctaatcc ttctttcatc ttgatgatta ttcctcggtt ttcatacatt
     5701 tcttttgtga gatgcgtaga aattttcgag tcttcaaaaa aagcttttct tacacgtgaa
     5761 gccattcgtt catcataaac cagtgtattg acctcaaagt ccaagcgaaa tgaacgaaca
     5821 tcgatatttg ctgagccaac tgaaacaatg ccaccatcga taatcatcgt ctttgcatga
     5881 ataaatccat tctcatatgt ttcgatcctt gctccatact cgatcagctc tgcagcgaaa
     5941 gaatacgttg cccagtaaac gagaagatga tctggtttgt taggtatttg gatatgaact
     6001 tttacgcctg aaagaagagc caacttcagt gcttcatgaa tcgatccatc cggtatgtaa
     6061 taaggcgttt ggatcaatat ctcccgtttg gctaacgaaa tcattttcaa ataagtcatt
     6121 ttgatttgtt catgttcggt atctggtccg cttgtcacga tttgaacagc tatatcacca
     6181 gtagtgtgaa tggacgggaa aaatttggga gaatagccta cttcttttgt atgctgagaa
     6241 ttccagtcca tcaaaaaacg gttttgcaag gtatacaccg cttctccgta aatacgcaaa
     6301 tgattgtctc gccaataacc aaatttcttt accaatccta gatattcatt tccaacattg
     6361 aacccacctg tatatccgat tttcccatca ataactacga ttttacgatg attgcgataa
     6421 ttgatccgag gattaatata cggaacaaat agaggaaaga aaaatgcgac ttctccgcct
     6481 gcttttttca aattctgaaa aaagctggaa gaaacttgtg tggaacccca tgcatcaaga
     6541 aggactctga cttttacccc tcgtcttgcc gcctctgtca atgcatctcg aacttcttca
     6601 cctaatgcat cactgcgata aatataatac tggaagtgga tatggtcttc tgcctgcttg
     6661 atatcttcaa tcagcgcatc gaatttttca cgaccatctg taaataaagt gatttcattt
     6721 ttagtcgtat agagcgtact ttcaaatacc gtgagcatat agatcaattg tttcacatct
     6781 acttgtccag taggcggatg aggataaagc cctcgcatca atgcctgttt gtcttcttca
     6841 atttccaggt tcttaccgat tttcccttgg atcttcaaat caaagatttt ctctttggaa
     6901 atccctcttc ctaaaaaaat atataaaata aatcctacaa ctggaatgaa catcaaaacg
     6961 agcagccatg cccatgtttg agcagtttcc tttctttctc gaaaaacgat gataaaggaa
     7021 agcaaaatat tcataatcaa aaggatcgta aaaaaattgt caacgataat tttcacggct
     7081 tcacctcttc ttctaccaac ttaagaatac ccaagttgtt ttttaaagta aagggaaaaa
     7141 agccgcttcc ttctattctc ctagccaata aaaaaagaaa gctggaacga aactgtccaa
     7201 ctttctcatt ttattta
//
LOCUS       DAZKOB010000125         6818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000125 DAZKOB010000000
VERSION     DAZKOB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.127"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..368
                     /locus_tag="ACG8M6_002589"
     CDS             <1..368
                     /locus_tag="ACG8M6_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458201.1"
                     /translation="HNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            686..1276
                     /locus_tag="ACG8M6_002590"
     CDS             686..1276
                     /locus_tag="ACG8M6_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0458202.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            1303..2613
                     /locus_tag="ACG8M6_002591"
     CDS             1303..2613
                     /locus_tag="ACG8M6_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0458203.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            2610..3329
                     /locus_tag="ACG8M6_002592"
     CDS             2610..3329
                     /locus_tag="ACG8M6_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0458204.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            3344..4429
                     /locus_tag="ACG8M6_002593"
     CDS             3344..4429
                     /locus_tag="ACG8M6_002593"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0458205.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            4565..6097
                     /locus_tag="ACG8M6_002594"
     CDS             4565..6097
                     /locus_tag="ACG8M6_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0458206.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            6275..6496
                     /locus_tag="ACG8M6_002595"
     CDS             6275..6496
                     /locus_tag="ACG8M6_002595"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0458207.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         2394 a          735 c         1309 g         2380 t
ORIGIN      
        1 cacataattt tagagataat gagttcgatt gtaatattga attagttaaa aaaattgaag
       61 gcgatttaag ctatatatta gaaaatgaga tatttgtaaa caaaacaagg agacttttgt
      121 tgggacgctg tcgtacttgt attcaaaagg cagttagata tgctagtttt aataaagaat
      181 ttgatttaaa ctcctatttg tctaagatac ttatgaacga tgagttgatt gcctgtattc
      241 actcataccc atataaacaa aatccaatta aacaacgaat atttaactat tttttactaa
      301 accaatccat tattggggtt aaaacattag catatttaag cggaatgggt agaaatagga
      361 gattttaatc ataaaaaact gtactatagt gaatttttgg agatttatat tccagttata
      421 tattaatgat tgtgatactt gtactattgt aaaaattcgt taggtttaaa taactggaaa
      481 tatcggaaaa tgggcattat acatcacgga catcccttct tttggaaaaa atgttacgat
      541 ttttataagt aatattgtat ccggtggaat caacgatgaa gataacatat ccatcgttgt
      601 agattcagta gcattgaatg atgctcttgt tgcaagaaat ccgaataaaa ttgttaagta
      661 taaattgaat taataggaga tatgaatgga aattagcagt aaagaatatt taaaagcggt
      721 tcttcgtatg gaaaagaatc tatatataga atctaaagga caacacacaa aattactaaa
      781 aataatgaaa tcacatccgg actacaacgc ttggcggtat actcgtcgaa tgagaattac
      841 atcttattat tattcaaaga gaaagaaaaa tatcatttat gcgattatgt atattgtgtc
      901 ttgcagaaga atgaatagtt tgggaagaaa atgtggtata gagtgtggtg aaaatgtatt
      961 tgatgaaggt gtaagatttt accatacaca aggaattgtt attaatggta acgccataat
     1021 aggaaaaaat tgttacttat atgggaataa ctgtattgga aatgatggaa tcgatcctaa
     1081 gtgtcctgta attggaaata atgtacgtgt atgcgtgggt gcaaagatta ttggtaatgt
     1141 aaaaatagct aacaatgtag ttgttgcggc aggagctatt gttatcaaag attgtctaga
     1201 gaatggtgca attcttgcag gtgtaccggc aaaaataata gggtatgtta atttaacaaa
     1261 agtattttta gaataatttt tagcgaaaga aggtgaataa cgatgcgtat ttcaataaaa
     1321 agatggtcag gagcagattg gacattgttc acgttggcat tttttgcaat ctccatagtt
     1381 cttgtatata cttttaacgc cccaagagca attttatatt tgggagatat aattaatatc
     1441 tttgtgttat taaatgcact tagctttaga cgcagaaaag gaatatatgc tagcaaaaat
     1501 gagatgctga tagttttgtt tttactagtt tctatttgta gctcagttat taatttagaa
     1561 aatataggat tgattatctg ggggatacga aataacactc gtatgttcat agtatatata
     1621 aattgtgcaa catatttaac aattaaagat tactataaag tattcgctat aattgaaaaa
     1681 ttattctgga tttcactgcc actttgttta attgaaagat tcatggtcac ctatccaact
     1741 ggaacaattg taggagatat gataggtgga gttttttgga attattcagg ttgtaatatg
     1801 ccattgaatt taattttgtg tgtgacagtt actgttgtta caatcggata tttcaaggga
     1861 gaaaaaaatt tcatccattt tattttatgt gtttgtgcag cactttttat gtctgcatta
     1921 gctgaattga aagtttttat tcctgaatta tttataatat ttgttgcaat agtattcact
     1981 ttaaaagttg attttaaagt agtgatcaag atattatcat gcgtaatact tgtgtcgata
     2041 gtattatcct tttttataac aatatttatt acattgaacg ataatggaca aagctatgct
     2101 gataatttca caattcaagg tcttttagaa tatgcgtcaa gagattctgg atatgatggt
     2161 gttggtgatt taaatcgaat aactggtatc tctagagtag tagatgaaat atttaataat
     2221 aattttagat tagtcctaat gggagtaggc attggcaatg cagagtttac caattttttt
     2281 gtcagtgagt tttataaatc gtttggtaat cttcattatc agtattttca tcatatttgg
     2341 ttatttatag aagttggaat attaggagtt tcgatattcc tattttttat aataaagaca
     2401 tttttcaatg caaaaagatc ttttatgaag gataaagatg caagtcttgt taaagttatt
     2461 gcaattatgt cggtattctt ttttgtgtat aacacaacac ttaggggtga aacaacgggg
     2521 ccactgattt atatgatttt agccatgcct gaagttgtga aaaggaaaga gcattattat
     2581 agtgaaaaaa atacaaaagg agagatatta tgattcctaa gaagatacat tactgttggt
     2641 tcgggggaaa tccacttccg aaaaaagata gaaagtgcat agaaagctgg aaaaagtatt
     2701 gccctgatta tgagattatt gaatggaatg aatcaaatta tgatttaaat aagaaccgtt
     2761 atatggccga tgcatataaa gaacgtaaat ggggttttgt tccagattat gcaagatttg
     2821 atattattta taatcatggt ggattttatt tagatacaga tgttgagcta attaagtcgt
     2881 tagatatact tcgtgaaaat catgcctata tgggatttga aggggaaatc tgggttaatg
     2941 gcggtattgg ttttggtgca gaagaaggac attctgttat taaaggttta agggatattt
     3001 acgattcatt aagtttttat caaaatgatg gtacattgaa cttgaaaccg agtccgtatt
     3061 atattacaga ttatcttgaa tctattggat tggttcgcga taacagcgaa caaataataa
     3121 atggcctgca tatctatcct acggattatt ttgctgccaa ggattatgat actggaaaaa
     3181 tttataaaac tagtaataca ataagcattc atcaatataa tgcatcttgg aaaacaccta
     3241 aacaaaaatt tgtttataga ttaaaatcaa caattggaca aaaaaaattt aacaggttac
     3301 ttgcactgaa aaaattttta cacttataaa ggagaaaagt actttgaaga aaatttgttt
     3361 cattagtggt gtaatttctc gttctggggg aactgaaaga gtagggagta ttattgccaa
     3421 tagcttgtct gatttaggtt atgatgtcac tattttaagc ttttggaata gtggagagcc
     3481 ttattttggg ttagataaga cgattaaggt tgattatatg ttaaatccta aaacggaggg
     3541 aaaattatat agaacttatt tatatccggt ttggaaattg cacaagtata tatgcaaaaa
     3601 tgaatttgat atagtaattg atattgatac tttgttgtct ttgtatacta caagggcaat
     3661 taaaggaaca aagtgcaaat tgatttcttg ggaacatttt aattactgga caatggaaat
     3721 gctgaaagaa aagaagagat tcaaggctaa aaaattgata aaaaaatatg caaagaggct
     3781 tgtggttctc acagaagaag atcgtcaaaa acatattgat gaatatagtt tatcagaaga
     3841 ttttgttata actatgccaa atccgtgtat atctaatatc gagtacgaat atcattatga
     3901 taaaaagaga tttcttgcag tagggcgatt agtggaagag aaaaattttg ctgacatgat
     3961 aagtgcttgg aaaattgtag aaaaaaaagt agatgattgg gatcttatca ttgtaggaaa
     4021 aggtgaatta gaaaaagatc ttcgccagca gataaaagat ttaaatctgc aaagagtaac
     4081 tatagcaggg cattcagacc atgtggagga atattataaa tctgcatctt gttatgtgct
     4141 tagttctgtt tatgaaggat ttcctatggt tatattagaa gcacagtctt atggattacc
     4201 agttatttct tatgattgta aaactggtcc gagagatttg gtacatcata attttaatgg
     4261 gatgttggtt gaagataaga atatagacca attagctaag agcatgataa tgtttactaa
     4321 gaatactgat ttagcaatga gaatgtcact gaacgcatat aataatgtac aaaaatttaa
     4381 tttgaaagaa attacaaaac aatgggttgc tttgattgaa aatgtatagt tttttgtggt
     4441 tgtccaaaca tgaaaattag cgtaattaat caaagagcta gaagaaacct attacatttc
     4501 aagattttag aaattatata gtattaggta acaataaaaa atagaaagtt tggagccata
     4561 tgatatgata attaatcgta aacaaaatgc taaaaggaat ataattactg gaacaatttt
     4621 taagttatat cagattatca ttcccttttt tatgcgtaca cttatgattt atcagatggg
     4681 aatggagtat gctggattga acaacctgtt cacatctata tttcaaatgt taaatttagc
     4741 ggaattggga ataggtgctg cactaactgt aagtatgtat aaacctatag cagaagacaa
     4801 tagaaaagag atttgtgctt tactgaacgc atataaaaag tacttttcaa ttattggatg
     4861 gatagtgttt acattaggat tgttatgcat tccttttttg agatttttag ttaaaggttc
     4921 gattcctgga aatttaaata tgacagtttt gtatttaatg tatttgataa atactgtttt
     4981 gtcgtattgg ttattttcat atagaaagag tctaatatat gcacatcaaa gaaatgatgt
     5041 aattaacgtg tctatgctaa ttacttttac attccaattt gtaatgcagg catatgttct
     5101 gattttctta aaaaattact atttgtattt attggcttct attttgggac aaatagttct
     5161 taacttaatg tgtgctgttg tttctcaaaa gatgtatcct gattatactc ctgttggtga
     5221 gttagagaaa gaaactattg acgatattat aaaaaaagta aaaggattag ttacaaacaa
     5281 ggtaggcggt acaatattac gatcttcaga ttcaattatt atttcatctt tcttaggtct
     5341 ttcagtactt gcgatttatc agaattatta ctttattatt tctgccataa tgagtatatt
     5401 tgcaataatt tatgaatcat gtatagcagg tataggtaac agtcttattg tagaaagtca
     5461 agaaaaaaat tatatagatt tttgtgatat tacaacaatt ataggttggt ttctggcaat
     5521 tagttgtgca tgctttatta cattatttca gccgttcatg attatctggg tgggaagtga
     5581 taatcttatg agtatttcat ttgttatatt tatgatagtt cattttttct tatatgaaat
     5641 tgatcagtta attgggttat acaaagatgc tgctggaata tggtacgcag acaggtttag
     5701 accattaatt acagcaatga taaacattgt attaaatatc atcctcgtta attggttcgg
     5761 attatatgga gttttggcgg caaccattgt ttcagtattg ttggttgatt taccctggtt
     5821 attatataat gttttccaaa atatatttaa atttgaatcg gcaggtaaat atgtttggaa
     5881 gctcataagt ttgtgtgtaa tagcttttgt tgcttcttat ttaagttatt ttgcttgttc
     5941 gtatgttaat atcggaaatt tcaaagtgat aagtttagtg ataaatttag ttatatcggt
     6001 tgtcatatcg attttagtta ttcttctgtt taatatccgt aataagaagt ttaagtatat
     6061 tttgaaagtt cttacgtcgg tattggtaaa aagatgagaa gagacatcag aatgatatat
     6121 aaagtaacta caatttttag gctgccgaat aggcgaacga tgtatagttt ttgttccaac
     6181 gaagactctt gttgaaattg gagatgatat gaaggagtta caattttgac tcatgtttat
     6241 gattgatcgg ttttaaaagt agtatggaga agctttggga tcatcggaga ggatcaaaat
     6301 aggtaataat gtttttatag gaatgaagtc tagaatacta aagggtgtac atataggaaa
     6361 taatgtagtt ataggtgcaa atagtttagt aaacaaggac gtatcagacg attgtgttgt
     6421 tgcaggaaat ctagcaaaag taattatgtc cctagatgaa tattataaaa agataaacaa
     6481 tatgaagaat ccgtaaaact tgtgaaacta taccgagaat aatatgggaa aaatattgat
     6541 gaaaaagcat tatatgagtt tttagggctg tttacagatg gagatgacaa tttgtcgaag
     6601 agttgggagc aatgcaaaat ttagtagggg attataaaca gactgacaag gtatttaaag
     6661 aacatgcaca agtatttcat aatattgaag aatttttgaa tactgtgaga taggagttgc
     6721 tacattgaga aaagtatgta tttttccaat gtaaaagatt acgagtttga aaacgacacc
     6781 ataatattat tttttaagaa gatattatag agcttagg
//
LOCUS       DAZKOB010000126         6698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000126 DAZKOB010000000
VERSION     DAZKOB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.128"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..565)
                     /locus_tag="ACG8M6_002596"
     CDS             complement(107..565)
                     /locus_tag="ACG8M6_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0458208.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(578..1909)
                     /locus_tag="ACG8M6_002597"
     CDS             complement(578..1909)
                     /locus_tag="ACG8M6_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0458209.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1920..3362)
                     /locus_tag="ACG8M6_002598"
     CDS             complement(1920..3362)
                     /locus_tag="ACG8M6_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0458210.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3349..4113)
                     /locus_tag="ACG8M6_002599"
     CDS             complement(3349..4113)
                     /locus_tag="ACG8M6_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0458211.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4217..5092)
                     /locus_tag="ACG8M6_002600"
     CDS             complement(4217..5092)
                     /locus_tag="ACG8M6_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0458212.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5347..5431)
                     /locus_tag="ACG8M6_002601"
     tRNA            complement(5347..5431)
                     /locus_tag="ACG8M6_002601"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5395..5397),aa:Leu,seq:caa)
     gene            complement(5449..5520)
                     /locus_tag="ACG8M6_002602"
     tRNA            complement(5449..5520)
                     /locus_tag="ACG8M6_002602"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5486..5488),aa:Gln,seq:ttg)
     gene            complement(5530..5602)
                     /locus_tag="ACG8M6_002603"
     tRNA            complement(5530..5602)
                     /locus_tag="ACG8M6_002603"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5567..5569),aa:His,seq:gtg)
     gene            complement(5664..5748)
                     /locus_tag="ACG8M6_002604"
     tRNA            complement(5664..5748)
                     /locus_tag="ACG8M6_002604"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5712..5714),aa:Leu,seq:caa)
     gene            complement(5769..5839)
                     /locus_tag="ACG8M6_002605"
     tRNA            complement(5769..5839)
                     /locus_tag="ACG8M6_002605"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5805..5807),aa:Cys,seq:gca)
     gene            complement(5885..5956)
                     /locus_tag="ACG8M6_002606"
     tRNA            complement(5885..5956)
                     /locus_tag="ACG8M6_002606"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5922..5924),aa:Gln,seq:ttg)
     gene            complement(5968..6040)
                     /locus_tag="ACG8M6_002607"
     tRNA            complement(5968..6040)
                     /locus_tag="ACG8M6_002607"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6005..6007),aa:His,seq:gtg)
     gene            complement(6043..6116)
                     /locus_tag="ACG8M6_002608"
     tRNA            complement(6043..6116)
                     /locus_tag="ACG8M6_002608"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6082..6084),aa:Trp,seq:cca)
     gene            complement(6123..6203)
                     /locus_tag="ACG8M6_002609"
     tRNA            complement(6123..6203)
                     /locus_tag="ACG8M6_002609"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6167..6169),aa:Tyr,seq:gta)
     gene            complement(6205..6280)
                     /locus_tag="ACG8M6_002610"
     tRNA            complement(6205..6280)
                     /locus_tag="ACG8M6_002610"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6245..6247),aa:Phe,seq:gaa)
     gene            complement(6316..6388)
                     /locus_tag="ACG8M6_002611"
     tRNA            complement(6316..6388)
                     /locus_tag="ACG8M6_002611"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6353..6355),aa:Asp,seq:gtc)
     gene            complement(6398..6470)
                     /locus_tag="ACG8M6_002612"
     tRNA            complement(6398..6470)
                     /locus_tag="ACG8M6_002612"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6435..6437),aa:Val,seq:tac)
     gene            complement(6486..6557)
                     /locus_tag="ACG8M6_002613"
     tRNA            complement(6486..6557)
                     /locus_tag="ACG8M6_002613"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6522..6524),aa:Glu,seq:ttc)
     gene            complement(6572..6661)
                     /locus_tag="ACG8M6_002614"
     tRNA            complement(6572..6661)
                     /locus_tag="ACG8M6_002614"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6625..6627),aa:Ser,seq:gga)
BASE COUNT         2023 a         1475 c         1086 g         2114 t
ORIGIN      
        1 aacaaaaatc aaaatgatta aatctagtga ttgaaacata tcatttcaaa aaaattttgt
       61 aaattcaccc ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac
      121 aactatcttt aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg
      181 gagaaactac aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa
      241 tagctaacag tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac
      301 ttccttcgag ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac
      361 tttcgatcgt tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg
      421 catggatata tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa
      481 agacctcgtc ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa
      541 tgtttcttgt tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt
      601 ttttgtttac tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg
      661 tttgattcaa tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta
      721 ctagtaactt ctctactgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg
      781 gttttcttag tgtaattggc tccacaccag ttgcaccaac atatgccggt atttcaacaa
      841 ctgcatctga ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac
      901 gacgatcatc attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa
      961 aattaaagtt caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg
     1021 tatttttctc tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct
     1081 caacgaccat atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa
     1141 aatttttcgt gatgactgac atcatttgaa aggttttttg ccaagtttta tctcctatat
     1201 taaaatcggc gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt
     1261 ctcgttgcaa ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat
     1321 acgtaacgat ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg
     1381 aaatagtcat atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt
     1441 cagaaacgat cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt
     1501 gtatatgatc gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc
     1561 cgagtccaca cgtttcttgt cctactaatc catgtttaag tggaatcttt tcgtcctttt
     1621 ctcgcatctc caatcctcca acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag
     1681 caagctttgg atcatccgtt gcacgaatgc gaatatttga aaaacctttt cttttcagca
     1741 taaatttaac aatcaagtac atatccatat ttctttcttc atcaatatca tataaacgta
     1801 tttcagataa tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat
     1861 acgtactccc gcctccagct atagtgataa tctgtttttt catcatcatc ttttcctttc
     1921 tattcttcta ataatttatt gaattctgcc ataacataag tcacttctaa gccgatgata
     1981 atttgataat ttttttcagt tggtttaatg atcccgctta tccctaattg cttgattcgc
     2041 tgttcctcga ccatggtggg atccactacc tctacccgta ggcgtgtatt acaactatag
     2101 acatccagga tattggcttt tcctccaaac gcttgtagca tttttgttgc catatattca
     2161 taatttttac ttgctaaccc taactctgtt tcttctaagg ttacttcatt gccaaactct
     2221 gccttagctt cacgtcctaa tgtctgaatg tttctcttat tgatcaaata ataaaaagta
     2281 acgtaataca aagcaaaaaa gaaaattccg attggaagaa tcaaaatagc attggtagcg
     2341 attcgaaaat tgatgagata atcaacaata catgctccga atgtaaaacc aattcgtata
     2401 ttgaataagt aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac
     2461 ataggagaaa gaaacatgaa actaaattca acaggctcag tgatatttgc aataaatgaa
     2521 gttcctgcac cgcttcccat gattcctttg accatctctt tattttcttt ataagctgct
     2581 ttatagatag ctagacatat agctggtata ccaaacatca ttactacaaa aaaaactgat
     2641 aaaaacagtc cagcctgagg atctccgttg ataaagcgtg tcatctctcc tctgaaaact
     2701 tccttgctag gagatgtata gcttcctaaa tcgtaataaa tatacgtatt caacacatgg
     2761 tgcagtccaa aggggatcag caaacgattc aaaaacataa atatccccac acctaatgct
     2821 cccatatcaa cgagcagttt tgcaaaatta tcaatcccaa tttggatgag cggccagata
     2881 aagcccaaaa aaacagctgc gattgtttgt agaatcatca ccatagtcaa tgggaatttt
     2941 tcgcccgcaa aataagaaaa aactactggt aatttttgtt ctttaaagcg attatacgtc
     3001 catgccgcta ctaaaccgga aataattcca ccaaaaatcc ccatattcac ttctgggtcc
     3061 ataatttcta agcctttttt caaggtagca atcgctaaaa aaccagtaag tgcaggtatt
     3121 cctttgtcct tatttttgac aaaaccgatt gcaactccta tagcaaataa gatatccaag
     3181 tttccaaaaa cggcatttcc agctgcacca attactggga tgttcagcat atcgtcagct
     3241 gataaacgca tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag catcgctctt
     3301 ccaagcttct gcacatttga tttaacattc atttttattc ctcctctatt aaatatcctc
     3361 atatgttact aactcaatca aaggatcatt tttcactttt tgagcaaatg ctgaatgaat
     3421 taaaaattcg acttggtggg tacgaggata cacaaaactt gaaccattta acaattcctg
     3481 atccaaatac gctgcgtgac acatgacttc gatcgtttta tcttctctca atatcatgag
     3541 taaacttgcc aaatattctt ccacactcgt attgatttgt cgtttttttt catcagtttc
     3601 tcctacgaca tcgaaacaag gctcaaaata atcgacatga cgaacttcat cttttcgttc
     3661 aaagttgctc cgaaccggca aatcatattt ttttgctaat gtaacaacta cctcctgatt
     3721 ttcatccaaa gtatgagtat gatgatgact gtctaaatga gttggtatga tccccgcatg
     3781 ataaattttt tgaatctgag cattccattc ctgatacagt tgatctttgt caatgttaaa
     3841 tggatgctga taataagata aggatctgaa ctctccgctt tcgataagag tatcgacagt
     3901 tttgaggagt ggtttgccac acgtcaatgt taaatggact cctacaccta gcctaggcat
     3961 ttcctttctt agtttcacag catgctcgaa acccggcata tttgccatca atgtagttga
     4021 tgtcaaaatt cctcgctgat aagcatccat aatcccataa ttcacaccat ggctatagcc
     4081 aaaatcatcc gaattaaaaa tcacttttcc catttcattc tccttctttt ttctactaaa
     4141 ataggcgcaa ccaaataatc atagtccatt tactatgtgt catttggttg cgcctataga
     4201 tagttacgcc cttattttaa tagattcttg ctagatgaat tgccaaatag atgatttcat
     4261 tttcttgaat ggcaagttga aattgctgtt cgatgatatc tttaatcttt agtgctgtac
     4321 aatacgcttt gctatgttta aatgtttcag taaaaaaacc aatattttct actggcgttt
     4381 cttcctgata aatcaagcgt ttaaccagga atttgatatg aaccattaag cgatgataag
     4441 tcagtgattc tttatcataa acgtttgaat aggatttttc tagatgggac aaaatcgtgg
     4501 tcgtcatttc tactattttt aagctcttta aactattagg ctctcctagc tcagcattaa
     4561 tgatatgcat agtgatgaat cctacttcat ttgaccctaa ctcggtttga tatcgatgat
     4621 tgattcgctc gaccacttct actgctatat catattcatt tggataaagg aatctcattt
     4681 ctggcaacat aattgctgtg acatctctat cttctctttt tcgttgaata gcaaaataaa
     4741 tatgatctgt caatgtaata tagatatttg aatctaatgg agaggagatc ctagctgtta
     4801 tcagttcaat cacttcttcc gcaaaatcca taaattcgat tggaatctgc gagactatct
     4861 cactgaatcg tttgatcttt tctttagaat ctaatacaaa taatttgtca gcatcctcaa
     4921 catcaaatgg cttactcact ttagcagaaa agccaatccc tttccccatc gcaatttttt
     4981 ctattccatc gtcatcgatc aatacagcat tatgattcag ctttttcatc acatacaccg
     5041 ttaattcacc tcctctatat gataaaacaa aacaacttgc agactccttc atacatattt
     5101 ttgtctccaa acaattttta aagacaaaaa acgacctaag aaagaaccct ttcttaggtc
     5161 gtgcctaatt gaatagtagc accctacata atttacaaaa atagtttaac acataataaa
     5221 agcgttatca atatcttttt tactcgtttt atgataatac ttgtatcgaa ttgatttgga
     5281 ggtaattaca taaaaacaaa aaaaatatag caactctcta taagaaagtt gctatattga
     5341 taattatacc ggcggccggg gtcgaaccgg cacgtcctca acggacactg gattttgagt
     5401 ccagcgcgtc tgccaattcc gccacgccgg caaatttaaa aataataact ggggtagctg
     5461 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
     5521 agaataaaag gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg
     5581 ttaaccactt cgccacattc gccatcataa aagatgttat ttaatttctc aataaaaaac
     5641 taagatgaag acatcttagt caataccggc ggccggggtc gaaccggcac gccctcaacg
     5701 ggcactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa gttgatttat
     5761 tagtactaag gcggtaaccg gatttgaacc ggtgatgaag gttttgcaga cctctgcctt
     5821 accacttggc tataccgcca ttatactatt atcaatctat aaaataaaga ataaattata
     5881 gaaactgggg tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc
     5941 gcttggctat accccaagaa taaaaagggc gaatgatggg aatcgaaccc acgaatgccg
     6001 gagccacaat ccggtgcgtt aaccacttcg ccacattcgc catggcaggg gcagcaggaa
     6061 ttgaacccac actaacggtt ttggagaccg ctgttctacc tttaaactat gcccctataa
     6121 aatggaggag agtggattcg aaccactgaa ccctaaggaa cggatttaca gtccgtcgcg
     6181 tttagccact tcgctactcc tccatggtgg cgcgggacag aatcgaactg ccgacacacg
     6241 gagcttcaat ccgttgctct accaactgag ctaccgcgcc atattttaaa attattaatt
     6301 gaataacaag aacgacggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca
     6361 ggcatgttaa cccctacacc acggaaccag ttcattatgg aggttaacgg gatcgaaccg
     6421 ctgaccctct gcttgtaagg cagatgctct cccagctgag ctaaacctcc aacattcaat
     6481 taaaatgacc cgtacgggat tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac
     6541 cgcttgacca acgggccagg taaatttgct acggagagta agggattcga acccttgaga
     6601 cagtttggca ccatctacat gatttccaat catgctcctt cggcctctcg gacaactctc
     6661 cagagtgaaa gaagctcaat actctttctc aaaatgag
//
LOCUS       DAZKOB010000127         6679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000127 DAZKOB010000000
VERSION     DAZKOB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.129"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            217..3444
                     /gene="fss3"
                     /locus_tag="ACG8M6_002615"
     CDS             217..3444
                     /gene="fss3"
                     /locus_tag="ACG8M6_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0458213.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3557..3871
                     /locus_tag="ACG8M6_002616"
     CDS             3557..3871
                     /locus_tag="ACG8M6_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0458214.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3884..4258
                     /locus_tag="ACG8M6_002617"
     CDS             3884..4258
                     /locus_tag="ACG8M6_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0458215.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4268..4603
                     /locus_tag="ACG8M6_002618"
     CDS             4268..4603
                     /locus_tag="ACG8M6_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0458216.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4686..6026
                     /locus_tag="ACG8M6_002619"
     CDS             4686..6026
                     /locus_tag="ACG8M6_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0458217.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6104..>6679
                     /locus_tag="ACG8M6_002620"
     CDS             6104..>6679
                     /locus_tag="ACG8M6_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0458218.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYT"
BASE COUNT         2240 a         1112 c         1467 g         1860 t
ORIGIN      
        1 tattttatta tatttgatta aatttgaaat gtttgtgttc taaaatgtgt tctaatttaa
       61 aggcagtata tttcgtattt tagattaaat ttttaggctc attcattctc aaatccattc
      121 tattcagtca ataaaaattt taaaaagact ttataaacaa agatgttcaa gcgagcatct
      181 tatttttttg tgcttaattt gaaaggagaa catttcatgt ataaaaaaca aaaaaagaaa
      241 ttatgtgtat taactatttt gagtttggct gttcaagcaa ttgttggagc aattgctcct
      301 acgattactt ttgctgatga aattacccat ccacaaacgg taaccgtaga actagattta
      361 gcacatcaat atgctgttga aggaactttt agtgatggtc gtccaatgtc agaagtcact
      421 gttccgcatt atgcagtgta caacggcttt aaacaagata ttttctgtat tgaaccagga
      481 gtcccgattt acaatgagtt cactcctggc tatgagaaaa acccgttacc agatatgtcc
      541 gataaagcca aattagtttc cgttttatgg aaaaaagtag gtacagatgc agatactcat
      601 attgtggcac agaagatgat ctggcaagaa gttaatgggt atacccttca ctcaataaaa
      661 cgatcagatg gtagtgccgt aaatatcgca gccattgagg caaaaataaa tcaagccatt
      721 gacgattatc agaagaaacc aaccttccat aatagtacgg ctaaaacagt attagggcaa
      781 tcgactactg tgacggatac gaataatctg aatttgtcag agttcgatga ggttgtggaa
      841 aatgccgcaa acattgatta tcgtgtgaat ggtaatcagt tgataatcac cccaaatgcc
      901 aattcaaaag aaagtggtgt gctgacgctt aagaaatctg ctggtaccgg aacacctgtc
      961 gcctataaaa aggctggcca acagacttta atggccgggg cgattgataa acctaatacc
     1021 tacacaatca aaattgatgt ggaaacggag ggttctttaa agatcaaaaa agtcgataaa
     1081 gaatcgggtg atgttgtacc aggaacggtt ttccatttag actttggaaa aactttacct
     1141 gcaaaagacg tgactactga caaagaaggc attgctacat tggatgagat tcctcatggt
     1201 acaaaagtaa ccattaccga aaaatcagta ccggcgcctt atacgattga taccaccccc
     1261 atgactacca ccattaaagc aggcgaaacc atttacgtga cttcaaaaaa cgcacgggaa
     1321 aaagggcaaa tcattctgga taaaagcgga gttgaaacag ggagtgatct ttggaatgac
     1381 aattattcct tagctggtaa tacgtttgcc attcgcaaag acagtccaac cggtgaaatc
     1441 gttcaagaaa tgaccacgga tgaaaacggt catgcggaaa caccaaaaga gattgctaat
     1501 gcgttggaat tgggaaccta ttatgtgacc gaaactaaag ctagtcatgg gttcgtgaat
     1561 acgttcaaac ctgtaaaagt cgaattgaag tatgccaatc aaaccgtagc ccctgttacc
     1621 agtaacgtaa aagggcaaaa tcaagaagtc actggcgaaa ccactttgac gaaagaagac
     1681 aaagatactg gggataaagc acaaggtaag gcagtctttg agggtgctga atacaccttg
     1741 tttactgcta aggatggaaa agctgttaaa tggagtgagg cttttaaacc agagttagcg
     1801 aagggaacga aaggttctga tgaaacggtc actttgactt tagacgaaaa gaaccaagtt
     1861 gccgttaaac atttagctat caacgagtat tattggcaag aaacaaaagc gcccgaagga
     1921 tatagccttg atgaaacaaa gtatcctgta tccatcaaaa aggtggatga tagcgaaaaa
     1981 aatgccgtga ttacccgaaa tgttacggca aaagaacaag tgattcgctt tggctttgat
     2041 ttctttaaat ttgctggatc agctgcgggc actaccgaaa ctggcttcaa tgatctgacc
     2101 tttaaagtgt cgccattgga agggaccaat gaaatcacag gtgccgaaga tgaagcgacc
     2161 acagcttata acgagcaatt agggtttgat ggttatggca agtttgaaaa tcttccttat
     2221 ggggattatt tacttgaaga agtagaagcc ccagaaggtt ttcaaaagat tacgccatta
     2281 gaaatccgtt ctacttttaa ggaaaacaaa gaagactttg tgaagagtga gtatgtcttt
     2341 accattaccg aacaagacca aaaacaacca attaaaacgg tgaccgttcc ttacgagaag
     2401 ctgacgaaca aagcattttc tgttagtttg aaccgtttaa tgctctatga tttgcctgag
     2461 gaagaagata gtttaacttc tcttgcgact tggaaagacg gaaacaaaga attaacttct
     2521 ctggattcta ccgagctagt tgataaattg agttacaatc tacatgaaat caaagaagac
     2581 tggtatgtgg tagcgcaagc cattgatgtg gatgcgacaa aagctgccca agaaaaagat
     2641 gaaaaagcca aaccagtggt gattgcagaa acaagcgcaa cgttggcgaa caaagagaaa
     2701 acaggaactt ggaaaattca gcataaatta accgccgaac aagttttaaa taaaactatt
     2761 gtcttattca actatgtgta tgaaaataag gaagcttttg aagcaggcga taagccagta
     2821 gcgaaggatg ttagcttgaa taaccaagcc caaactgtca gttgtacagt ggaacaccat
     2881 gtttctatcc aaacaaaggc ccacctagaa aacggttctc agacctttac tcatggtgat
     2941 gtggtggata tgtttgatga tgtgtcgatt actcatgatg tactggatgg ctcaaaagaa
     3001 gctttcgaaa caattctgta tgctttatta ccggatggta caaacaaaga aatttggaaa
     3061 tctggcaaga ttgattatga agtaaatgat aaagaattca ccaaaactgt gcttgcgaaa
     3121 aaagtagata ccggaaaata tccagaagga accaagttta cttttgcgga aatcaattac
     3181 gataaagatg ggaatatcaa tggaaaacac aatgaagatt tgaaagaaaa atctcaaacc
     3241 ttaacaccaa aagaagttcc aaccatacta agtacgccga aacaaccgga aacaccaact
     3301 gttccaagtg gctctcaaga atctagtccc acagtgaaga ccttccctca aaccggggag
     3361 aaaaattcca atgttttact gttcattggt tttactttga tttttgcgac ggcgggctat
     3421 tatttctgga atcgccggaa ctaaggtgat gagatgatac aagtaaatcc taagaaccaa
     3481 cgtgcgcgac cgcctcctaa aaaagtagtc gcgctatttt tgtatccaaa actaacaatg
     3541 aaaagaggaa aagaagatgg aattgaaatt tgtcgtgcca gatatggctg aaacctttgg
     3601 aaagttaagt tatgccggtg aaggagaaat tataacagaa ggatatggac ggaatactac
     3661 ggtgattggt cgcagttatc atttgtattc cagtaagcaa cgggccgatg atattgaggt
     3721 ggtagtagct gcggaagccg gtgaaaagga ttttgatcaa gaccaaccgc taaaagccgt
     3781 gaatccccat ttggttgcca aaggctatga gattgagaat cgtgggttta ctgattacgt
     3841 gttgtatgtt gatgatttag tgaaagcata ggaggaaaag aagatgagat taacagaagg
     3901 tattgtcgta gattcaggtt taacatttgg gaagttgcgt ttttccgcat tacgccgaga
     3961 agtacggaag caaaatgagg atggaacaat tggtaatgag gtaaaagaac gaacctataa
     4021 tttaaaatcc tctgcacaag ggcgaatgat tcaagttagt atcccagcta atgtaccctt
     4081 acgcgagttt gcttatgatg cagaggtaga gttggtcaat ccaatcgtgg ataccgttgc
     4141 caattatgtt tttcgagaag gaactaccgt taattggttc attaaggcgg atgatttagt
     4201 cttgaagcgg cagccgaatc aaggaaatcc tacgaaccaa aatgaaagaa agaaataggt
     4261 gaagattatg gaaattaatg ttgaacatat tttagattgt ctcgatcaat atggaaaagg
     4321 cgagctgact gaggaacaac tcgttaaagc attaacctct gatgaaaaaa tgtttctgat
     4381 tatacatcaa ggtttacttg gagaaaatag cgacagtgaa gaagactctg atgtactaga
     4441 ctggttagga gaagaagagt ctttctttat agttgtcgaa gtaaatgaaa acttatgtcg
     4501 gcaggcagaa tccattttgg aagaaattgg tgtggagatg ccggatgcta ttgaaggctt
     4561 tttaaatcaa ttggttgaaa cgaaacaact cccctcctcg tagcagtaaa tgactagcat
     4621 aactttttga aattgcctaa tgaaatccta ttgggctttt tcgattggag gtggaaacca
     4681 tcaaaatgta caaaggacat cgcatacgcg caggagatca gcacttggtt tatcactttg
     4741 ttcttggctg gctactcgct ctgttcatcg gttggatgag cgtcttttat tttcaagagt
     4801 ttagacaatt tgatatttct aagctatcac tttctactat tgaaatcgtt tggtctataa
     4861 aagatttagt atgtctgctt ggaagtctcg ctttttcagg agctatgata ttgctttata
     4921 ttcacttttt tcttgatcat tggcgaagct tgtggcatcg acaaaagcta gctcggatga
     4981 ttctagagaa ccattggtat gaagtgaaac aaactcaaag tgaaggcttt ttcaaagatt
     5041 tgaatagtag ccgaaccaga gagactatca gttacttccc caaaatctgt tatcgaatga
     5101 aagatggatt actgtctatt cgcgttcaaa tttcacttgg gaaataccaa gatcagcttt
     5161 taaaattgga aaagaagtta gaaagcggac tgtattgtga gttagtagaa aaggaactca
     5221 aagactccta tgtggagtat actttactgt atgatatgat cgccaatcga attgggatag
     5281 acgaagtagt ggcagaaaat gggactctgc gattgatgaa aaatcaagta tgggcttatg
     5341 attctttacc ccatatgtta atcgctggtg gcacgggtgg agggaagacc tatttcctcc
     5401 tcaccattat cgaagcacta ttgaagtcag atgcagaatt gtttatcctc gatcccaaaa
     5461 atgcagattt agctgattta ggtacggtga tgcctcatgt ttattcacaa aaggaagaaa
     5521 tttctgcttg tgtggaagat ttctatgaac gtatgatggc tcgtagcaag gcaatgaaag
     5581 aaatgtctaa ctacaaaaca ggagagaatt atgcctatct tggacttcca cccaactttt
     5641 taatctttga tgaatacgtg gcttatatgg agatgttaac gacgaaagaa agtgcggtga
     5701 ttttgaataa gctcaaacaa atcgtgatgt tgggtcgtca atctggtttc tttctgattt
     5761 tggcttgcca aaggccggat gcgaaatatt taggagacgg aatccgcgat caattcaact
     5821 ttcgcgtggc tttgggtcgt atgagtgaac tcggatattc catgatgttt ggggaggtcg
     5881 aaaaaaactt ctttatgaag cacatcaaag gtcgaggtta tgtggataca ggtggcagtg
     5941 tgattagtga gttttatacg ccacttgtac caaaagggta tgatttctta gattcaatta
     6001 gtaaattaga aaagatgaat gattgagtga tcttcaatac tgactttata cttcaagtaa
     6061 cgtataatac ttttatatag taggaagaaa ggggataatt aatatggaaa gtaatgattc
     6121 ttatttagag tatgcaaaag caaataagga taagtttatt tcttatatta tcaaagggaa
     6181 atcccccgaa gttggacgag acgcagtttt tatggcaggg agtcctgggg ctggaaaatc
     6241 agaggttgcc attggattag ctgagaatta tgataaccat gtgattattg atgcggatta
     6301 ctttcgcacc cagtttcctg attataatgg aaaaaactct agtctctttc aaaaagcatc
     6361 ttcttggttg gtagagcagg catttagata tgtcttagat catggctatt cttttattct
     6421 ggatgcaact tttgcaatat taagtgctga aaaaaatatc tcgcgggcgt tgaaaaaaca
     6481 ttttcgagtg acgattttct atgtttatca agaccctaaa atcgcttgga aatttacaca
     6541 agaacgtgaa attgcagagg gacgtcatgt gccgaaaaag gcatttatta acgctttttt
     6601 caaatcaaga gagaacattg caaaagtaaa gagtaaacat ccagatgtat tgcttcatat
     6661 cataataaaa gattatacg
//
LOCUS       DAZKOB010000128         6644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000128 DAZKOB010000000
VERSION     DAZKOB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.130"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8M6_002621"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8M6_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8M6_002622"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8M6_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0458219.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8M6_002623"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8M6_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8M6_002624"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8M6_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0458220.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8M6_002625"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8M6_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0458221.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8M6_002626"
     CDS             3582..4271
                     /locus_tag="ACG8M6_002626"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0458222.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8M6_002627"
     CDS             4285..5787
                     /locus_tag="ACG8M6_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0458223.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8M6_002628"
     CDS             5800..6276
                     /locus_tag="ACG8M6_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0458224.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8M6_002629"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8M6_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKOB010000129         6605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000129 DAZKOB010000000
VERSION     DAZKOB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.131"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..1042
                     /locus_tag="ACG8M6_002630"
     CDS             35..1042
                     /locus_tag="ACG8M6_002630"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0458225.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1054..2058
                     /gene="neuB"
                     /locus_tag="ACG8M6_002631"
     CDS             1054..2058
                     /gene="neuB"
                     /locus_tag="ACG8M6_002631"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFQ0458226.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2055..3203
                     /gene="neuC"
                     /locus_tag="ACG8M6_002632"
     CDS             2055..3203
                     /gene="neuC"
                     /locus_tag="ACG8M6_002632"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0458227.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3176..3853
                     /locus_tag="ACG8M6_002633"
     CDS             3176..3853
                     /locus_tag="ACG8M6_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0458228.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3804..4985
                     /locus_tag="ACG8M6_002634"
     CDS             3804..4985
                     /locus_tag="ACG8M6_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0458229.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            4998..5975
                     /locus_tag="ACG8M6_002635"
     CDS             4998..5975
                     /locus_tag="ACG8M6_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0458230.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            5975..>6605
                     /locus_tag="ACG8M6_002636"
     CDS             5975..>6605
                     /locus_tag="ACG8M6_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0458231.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYIL"
BASE COUNT         2463 a          827 c         1260 g         2055 t
ORIGIN      
        1 tatagatgac tataattcat aggaggagat ataagtgttt aaaggaaaaa caatattagt
       61 aactggcgga actggttcca ttggaagtga aattgttcgt cagcttttaa aatatgaacc
      121 aaaagctatc agaatttata gtagaggtga agaaaaacaa ttttatatgc aacaagagct
      181 ctcaaatcac actaatttga gatttttaat tggtgatatc agagactctc aaaggattga
      241 ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc gcaatgaaac acgttccagc
      301 aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg atgggaactc aaaatgtaat
      361 agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt atcagtacag ataaagtagt
      421 gagtcctact aacaccatgg gtgcgacaaa gttattgagt gaaaaattaa taatagctgc
      481 caatcagtat aaaggaaaaa cagaaacaat attttcttgt gtaaggtttg gaaatgtcat
      541 ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa ataaaaaaca ataaagatat
      601 tactttgaca gatgttgata tgacacgatt tatgatgtca ataccggatg ctgcaagatt
      661 agttctgtct gctgctatga attcaaaatt tggagaaaca tatgtcttaa aaatgccggc
      721 aatcaatatt ttaactttag ctcaatcatt aataaaaata tataatgata cacatgatga
      781 aaaatataga ggtgaaataa aagttgtagg tatcaggcca ggagaaaaat tatatgaaga
      841 gctaatgact acagacgaaa ttgaacgctc atatgaaaat gataaaatgt atatcattcc
      901 accacactat catgctactg acgaactgta caaagattat gaaaaaatta aaatcactgc
      961 atactcttca gatgcaacag aattaatgag tggacaggaa tttattagaa tcgcaaaaga
     1021 gaataatgtc gtgacttggt aaggagtttt atgatgaaat cagaagtata tgtaattgca
     1081 gaaattggtg taaaccataa tggaagtcta gatttagctt tagaaagtat tgataaagct
     1141 aaagaatgtg gagctaacgc ggttaaattt caaacattta aaacgaataa gctagtttcc
     1201 aaaaaggctc caatggcaaa atatcagaag gataatctta aagaggacag ttctcaattt
     1261 gaaatgctaa aaaaattaga attatctcaa tctgatttta ttagaataaa agattattgt
     1321 atacaaaata aaattgattt tttgtctact ccttttgatg aagaaagtgc agactttcta
     1381 aaacaaattg gtgtacaagg atttaaaatt ggctcaggag atttaacaaa tttacctttt
     1441 cttagaaaaa tagatatgta tgggttgcca gttttattat ctacaggtat ggctgatatt
     1501 catgaggtca gtgaggctgt agattgtttc atagatagtc cagttactat tttacattgt
     1561 acttcaaatt atccggctag ttctgaagat attaatttat tggcgattaa cacattaaaa
     1621 gaaatgttcg ggatacccgt tgggtactcc gatcattcat tgggatatga tgttggtatt
     1681 tgtgctgttg cgatgggtgc taaggtaatt gagaaacatt ttaccttgga taaggaacta
     1741 cctggtccag atcataaagc ttctttagat cctcaggaat ttgcagattt tgtaaagcat
     1801 attagaaaca ctgaaatatt tttaggtgat ggtgttaagc gagcaatgcc ttctgaactt
     1861 agtacaaaag aagtatcaag aaaaagccta gtggtcactg agaatttaaa tgtgggagat
     1921 attcttacag agaaatctct tacaataaaa cgtcctggaa atggaatagc tcctaagttt
     1981 ttatatcaat atattggcaa aaaagttaaa agaccaataa gtgcagatag tgttctgctt
     2041 gaggaagatg ttgaatgaaa aaaatactta tagtaagtgg aacacgagct gattatggaa
     2101 tatataaacc tgttgctgag aatttactac aagataattg ggatgtatca tttgttgttt
     2161 ctggaatgca tttatcccat aaatatggat ttactaaaga attgatctta gctgacggat
     2221 ttagaattag tggagaagtt caaagtttgt ttttagagaa caataaggga aacatggctc
     2281 ggtctgtggc attagagata aatggtttca caaatatatt tgaacaaact agccctgatt
     2341 ttgtattatt actaggggat agaggagaaa tgttggcagc tgcgatagct tgtttgtatc
     2401 ttggaatcaa aacagcacat attcacggag gcgaagtgtc tggtactgta gatgaatcaa
     2461 taagacatgc cataacgaaa atagcatctg ttcattttgc tgctacggaa aaaagcaaag
     2521 agcgtttaat aaaaatgggg gaagattctt ggagagtttt tcccgtcgga gctcctagaa
     2581 tagatactat tttaaataat agattaccta gttttagaga tataaaagaa aaatatcatc
     2641 taaaatttga aaagaaaaaa tacgttttgc aagtatttca tccagttgta accgaactag
     2701 aaaatataga aaaacaaatt ttagctttga tcaattcaat ggaacaacaa tctcaaccaa
     2761 ttatttgcat tttacctaat agtgatgctg gtagcgaaat tatcagaaaa atgtaccaga
     2821 aatatttaaa aacggatatt atttatatta cgaatttaga gccggaggaa tacctgacta
     2881 tattaaaaga gtgcaaattt atgatcggaa attcttcttc tgggataatt gaagctgcat
     2941 cattcaaaat accagtaata aatttaggaa ccagacaaaa tggacgagaa caatctaaaa
     3001 atattataaa tgcaaatcta aatgaaaatg aaattgaaac tgctttgaat aaaatacaag
     3061 atcctcagtt tataaaaagc ttgaaaaatg ttaagaacat ctacggtgac ggcaacagtt
     3121 ctagaagaat aacaaaaaca ttgaatcaaa tattgaatga tggggaagat attaagtgga
     3181 ttcaaaaaca aatagcttat taaaatatac tagtgatcca tacattatta ttggggctgg
     3241 cggacacgca aaagtcatta ttgaaacctt aagattagaa aataagttaa tatatggatt
     3301 ttttgatgat aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa taaacgaatt
     3361 gaatagctgg gcggatagca aaaaaaaaat ctataaattt attgtagcta ttggtgactg
     3421 tgtagtaaga aaaaagatta taaaaaaatt gaatatgcca agtgagcaat atggaatagc
     3481 tgttcatcca agtgctatag tggcaaaaga aatttcaatt ggttatggaa ctgttatttt
     3541 tgcgaatgct gtaattaacc cagatgctgt aattggagag catgctataa taaacacagg
     3601 tagcattgtt gagcatgatt gtagaattaa taactatgtt catttatctc caggtgtatg
     3661 cttagcgggt ggagtacatg taggtgtagg aactcaaata ggaattggtt ctcaatgtat
     3721 ccagatgaag aaaattggat cttggtgtat gataggcgca gggagcacta ttgttaagga
     3781 tattccctca cattccttag cttatggcaa tccagctaaa attaagaaag aaggaattaa
     3841 ttttgaattc tagaatttta ttatcaagtc ctcatatggg aggaacagaa gaaaaatatg
     3901 ttgctaaggc ttttaaagat aattggatag cgccattagg tgaaaatgtg gataaatttg
     3961 aagaaagtat tacaaaatat actggttcta agggcggagc ttctgcgtta agttcaggta
     4021 cagctgccat acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat attgtctttg
     4081 ctccttcatt tacttttata gctacagtaa atcctatttt atatcaaggg gctgtaccag
     4141 tgttcattga ctcagaacct aatacatgga atatgagccc tgttgcacta gagagcgcat
     4201 tagaagaagc tgaaagaaac aaccaattac ctaaggcaat catcgtagtg aatttatatg
     4261 gtcaaatggc tgattatgaa cgaattttag ctattgcaaa ccaatttaat gttccagtga
     4321 tagaagatgc agcggaatcg ttaggttcta cttataaggg aatttcgtca ggcttatttg
     4381 ggacaattgg gatctattca tttaacggta ataagatcat cacaacctca ggtggaggag
     4441 ctctagtgtc tagtgataaa aaatatgttg agcattcaag atttttagca acacaagcaa
     4501 gagatcaagc accgtattat caacatagcg aaataggtta taattaccgt ctaagcaata
     4561 tttctgccgg tatcggacgt ggacaaatgg aagtattgaa tgatagagta gctaaacgaa
     4621 gagaaatttt caatcgatat aaggaagctt tttctggaat tgatagaatt gaaatgatgc
     4681 cagaagtgga tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc aatgattata
     4741 attctattga tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac attgaaacaa
     4801 gagctttgtg gaaaccacta catttgcaac cagtatttca aggggtaaaa atgtattacc
     4861 atgataataa tcttagtgtg tcagaaaagt tatttaatag cggcttatgc ttaccttctg
     4921 gaagtaatat gacagatagt gaacaagaat ttgttattga tgagtttaaa aaaatattca
     4981 agtaggtgtg atattttatg aacataggaa ttattggttt aggttccatg gggaaaagaa
     5041 gacttagact cattcaagaa cactttaatg atattttact atgtggtgtt gatactaatg
     5101 aggcgagacg aaaggaagtt gaaaaggaat ttggaatacc aacttataat agtctaacag
     5161 aggcaaaaaa tttgtttgaa ataaattcag tttttgtttg tacatcccct ataacccatg
     5221 aatttattat aaaagaggct ttaaatttag attgtaatat ctttacggaa atcaatttat
     5281 taaatgaata ttatgatgaa ataatcgaag tagctaaaaa gaaagataga ttactttatc
     5341 tatcttctac atttttgaag agaaaagaaa ttcaatatat taagtctaaa gtgacgcata
     5401 ataaaaaaat ttcttatcaa taccatgtag gacagtattt acccgactgg catccttggg
     5461 aagattatcg caattttttt gtttcaaaca aaaaaactaa tgggtgtaga gaaatattcg
     5521 ctattgaatt accttggatt atagatacat ttgggaaagt taaatcgttt actgtaatga
     5581 cccagaaatt atccaatctt gatattgatt attcggacag tttcactctt attattgagc
     5641 atgaaactgg tatagttgga actttaagtg tagatgttgt ttcaagagta gctaaaagaa
     5701 atttaactat aattggtgaa aagattttaa tagaatggga aggtaacccg gattctctat
     5761 tttcatggaa tgaagaaact tctagtatga cagatattca attgtacgat gaggttatgc
     5821 atgataataa ttattcacat actattattg aagatgcata tttagaggag attaaagaat
     5881 ttttcacgct tttggaaaag aaaatggaac cccaatatac ttttgaaaaa gataagtata
     5941 ttattaactt aattaacgac attgaggtaa attaatgatg aaagtaattt tttttgggtt
     6001 agggtcaatt ggaaagagac atctaaaaaa tctaataagc tatggaaaaa agaattctat
     6061 taattttgat ataacagctt atagtagcag tggaaaaaaa gagtttgaag aaataacgta
     6121 tattcaaaat agagacgatt tggatacaca ttatgatata gcttttatta caaatccgac
     6181 ttttaagcat ctggaaacac taaatgtaat tgttgaaaaa gttgattaca tatttttaga
     6241 aaaacctgct ttttctgaga gtattgatct tactcaatat aattatgatt acaaaaatat
     6301 ttatgttgct gctccactga ggtataaaaa tatcatggaa gttgttcgta atagtattca
     6361 aagtaaaaaa gtatattctg taagagctat ttgttctact tatcttcctg attggagaaa
     6421 aggtactgat tatagaaata attattctgc ctttaaatct atggggggag gagtagaatt
     6481 agactgtata catgaattgg attatattat aaacctattt ggtttcccta aatcagtaaa
     6541 aaaacagttt ggtaaatatt cgaatcttga aattaacagt aatgatttag caacatatat
     6601 attga
//
LOCUS       DAZKOB010000130         6573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000130 DAZKOB010000000
VERSION     DAZKOB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.132"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..850
                     /locus_tag="ACG8M6_002637"
     CDS             <1..850
                     /locus_tag="ACG8M6_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0458232.1"
                     /translation="KLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAINRE
                     FWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIK
                     NAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNW
                     KATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGN
                     WKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKV
                     LKKIAL"
     gene            870..1430
                     /locus_tag="ACG8M6_002638"
     CDS             870..1430
                     /locus_tag="ACG8M6_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFQ0458233.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2319..3086
                     /locus_tag="ACG8M6_002639"
     CDS             2319..3086
                     /locus_tag="ACG8M6_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFQ0458234.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3112..3657
                     /locus_tag="ACG8M6_002640"
     CDS             3112..3657
                     /locus_tag="ACG8M6_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458235.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3658..4224
                     /locus_tag="ACG8M6_002641"
     CDS             3658..4224
                     /locus_tag="ACG8M6_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458236.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            4239..4487
                     /locus_tag="ACG8M6_002642"
     CDS             4239..4487
                     /locus_tag="ACG8M6_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458237.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4506..4745
                     /locus_tag="ACG8M6_002643"
     CDS             4506..4745
                     /locus_tag="ACG8M6_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458238.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            4762..5346
                     /locus_tag="ACG8M6_002644"
     CDS             4762..5346
                     /locus_tag="ACG8M6_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458239.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            5453..5734
                     /locus_tag="ACG8M6_002645"
     CDS             5453..5734
                     /locus_tag="ACG8M6_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0458240.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            5724..6065
                     /locus_tag="ACG8M6_002646"
     CDS             5724..6065
                     /locus_tag="ACG8M6_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0458241.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         2470 a          861 c         1363 g         1879 t
ORIGIN      
        1 aaaattaatt aaaaaggatg agattataat ggggttactg gttccgagag ctaatactta
       61 tactatacct ttaacgaaac gccaattaat tgctaaaaga tatcaaagga ttactagagc
      121 catcaataga gagttttgga attctgaaag tgatactgct cacagtttat acgtaggttc
      181 ttatgggaga ggaacggcaa ttagtactag tgatatagat ataatagttg aattaccgat
      241 ggcagaattt gacagattca aaaattattt atcaaatggc ccgtctaagt tattacaagt
      301 tataaaaaac gcatttcagg aaatactacc aaatagtgat attagagctg atggacaagt
      361 tgttaaaata aatttccatg atggaatcaa atttgaaata gttccagctt ttaatgaaaa
      421 agattattgg ggtgagtcaa aagggtttat ttatccagat tcaaacatgg gagggaactg
      481 gaaagctaca aatcccaaaa aagagcaaga agccatgaaa ttaaagaata caaaaagtaa
      541 taatttatta tatgctacgt gtaaacattt tcgacatgta cgagatacag aatttacgag
      601 ttatcatctt tcaggaatag taattgacag tttcgtatat gaggctatgg gaaattggaa
      661 attcgttgaa aataacagtg ggggtcaaaa cattagttct gtatcttatg agacagcttt
      721 attagaatac tataactcac ataaagttat gggcggttta aatttatact ctcctggaag
      781 caatcaattt gttaattctg atagtagtat catatgttta gaaaaagtat taaaaaaaat
      841 agctttataa agttttatgg aggaacaaca tgggagacga aaaaaagcgc ttggaagttc
      901 aattacgtga agcatatgga cgagtaacgt atacttacac cactcattta aagcaagccg
      961 ataatattat ttctgacaat aaaattttaa ggtgtcttca aattggtttg tcagccctta
     1021 ctactggagg ggtacttagt accattatta ctgataatag tgagttaacc tttgttgctg
     1081 gattatgttc aacaatttct cttggattaa acttatactt taagaatttt gatcttgctg
     1141 aagaagctaa aaatcatcaa attgttgccg ataaattatg gttgatcaga gaagaatacg
     1201 taagcttact aacagattta aatcgtttaa gtgttgaaga tattgtgaaa aaaagagatc
     1261 aattaatgaa cgaaactcat gaagcatata aaaatgcccc taaaacaaat agtaaaagct
     1321 atgaatcagc tcagaaagca ttaaaatctg aagaagagca attttttaca gaagaggaat
     1381 tggataagat gttaccagag catcttagaa aaaagagttc taaatattga actctttttt
     1441 tatgcttaaa attctcttgt aatttttcta ttcatctata tttaagaaga aatatgatga
     1501 tagaaacatc tcctaaagcc accgcaagcc gttctaagca tagcttagcc cgacattgct
     1561 agatatgctc atctcaccat gaacgtttcc taaagccacc gcaagccgtc ctagccattg
     1621 cctagtccga catcgctaga ttagtgcatc tcgccataaa agttttctaa agccaccgca
     1681 agccgttcta agcatagctt agcccgacat tgctagatat gctcatctca ccatgaacgt
     1741 ttcctaaagc caccgcaaac cgtcctagcc atcgcctagt ccgattttgc ttgatcggtt
     1801 ccatttctgt ttatttagtg ggtcaacacc gtaatcttaa atagccatcg cctatttaag
     1861 attactaggt ctgctttgaa ggtaaaaagt ctttcattct tcaagacttt taggctttca
     1921 agctacgcct gaaagcattg aaattcatcg ctctttattt ggttttgcca tttataattc
     1981 cttacgccca ttatcttttt cgcctgtcat accttgtcag aagtgtttag agcttatctc
     2041 atctttggat taatacaagt ggcgtaaatt tttttctaaa cgcaagcgtt acgaaaataa
     2101 tttacttaaa cacttgtatt aatccggcag ataagaacgc attctaatca ctcccgacaa
     2161 ggcaagacag gcgaaaaaga taaataaatg aacgaaagga agaaacataa aatgcaaaac
     2221 caaatagcga tgaagaaaaa accacctccc tctaaaacaa tccaaacaaa aaagaatttt
     2281 gaaaaaccaa aaatcaaaaa cagaaagaag cgttcaacat gagagataga attttagaag
     2341 ttaatcaaga aatggtaaac aagttaacaa atatcagtgg gaactatgaa aagtacgatg
     2401 gtactttact tgataactat atttttcacg atacacaaaa tatcagaatt ggcagaagta
     2461 aaccgagagc gttcattctc atcaaagaga aatatttgaa tgaatggaat agcacctatg
     2521 aaatggttat gactgataac tttgaaaagg tattggattt cagaaatgct ttgttgggtt
     2581 ctgatatggg aaatagcagt cttgaatcta ttgaagttga aaacaagacc gagaatgaag
     2641 gtgaaggatt gattaagaat gtcttgaaaa tcgaacaaaa atatatcagt gaagatagaa
     2701 gtttttatag agtcacaagc tcggttggtc tagcaaactt tgctactaac ttaattggaa
     2761 acaatgctag tgaatctttg ctagtgatgt gtctgaatac aaagaatgag gtcattgctt
     2821 atagcgaagt tttcaaaggt ggaattaatc aatcagtagc gataccaaga gagatttttc
     2881 aactagcttt gctaaacaat tcagcaaaaa tcgcagtatc acataatcat ccgagcggat
     2941 atgaatttcc aagtgaaaat gacaaaagtt ttactacgaa tttgaaaaac gcaggaaaaa
     3001 caattggtat tgaattgtta gaccatatca tcgtaacaag taaaaacaca agctattact
     3061 catttcaaga aaatggttct ttataaaaat tagagaaaag aggaaaacaa gatgggcgaa
     3121 agaacacaag cgttaattac agtcaaatat ggtaacagca aaacgaaagc aagcgtgtcg
     3181 attcattatc agtgggggta tggtcgatat atgctgatgg acgtattgaa tcaaggaatt
     3241 tatttaatca ataaagcata cgacttacga gataaatcaa gagaagaatt agcaaaagga
     3301 atctttgata gaagtacagg agttatcaat tttgtcacct atgaatctgg agtgagtgct
     3361 tatgatgaaa cagggtttgg tgggcgctat gaaccttatc aagaaacgat tgaagatgtg
     3421 atgaagtttt cagataacaa tgatggctat gctcatttag agatggtgtt cgatgaaaat
     3481 ggatttagtc tagatgataa aaccagattg cgattgtttg attttgaggg tcaacagata
     3541 ggactaaaca agttttgtga aagcggaaaa gagtttgcta cgaaagattt tattaaggga
     3601 tacagattat tgctagaaaa ttatggaata aacctagaag aagagagcgt gaaataagtg
     3661 tttgaaaaaa gaaaagacca gttccctaat ttagaagcga aaagtcaaaa agtgattgat
     3721 ggtgaaatga ttgaattatt ccaaaaaagt tatggaacca tagaaactac cgcaagttgc
     3781 aaatactact ttcaaggaaa gagaaacacg tttaaaggct ttgccgattc tgatgaagaa
     3841 ttggcaaaga aaatcattga ttggcttgtc agcagtgaac acgtagcaag agaagatacc
     3901 gttagaacaa cgattaatgg ggaagtccta acaggtacta gatgtgtttg ttgtaatcat
     3961 gaatttgtag aagatgaagg ttgctaccga atcaatgaat atggtgattt tatttgccag
     4021 agttgccacg agatagaatt tccaaccgaa agaagttggt ttgaatacat gctacaagat
     4081 tatgagatgg aaacagaaaa tggtgaagta gttttttgta ccaaagagga attgagtaag
     4141 agaagcgatg atgaaattga agattggtgt gtgtggttag tagggacaga tgattgcgtt
     4201 gcatattaca caacagcaga ataggagtgt gaatcagcat ggaaggaatg gattttatag
     4261 atcatgaaga tttgattgat tttggttata catggaaagg aatggtaggt attagcagaa
     4321 gtcttgctaa tgccttttac gaaagaaatt atgcagtcta tgtcctttat gatgacaata
     4381 cagaaagcct tgttgatgaa gagtataaat tggatttaga gaatgtttta tatgggatag
     4441 aaaaagaaga tttagcaaaa tatatttttt catggttggg tcaataaacg aaagagggta
     4501 gcaacatgac aaaattcaaa agtaaaggta tgcaatggtg ttcttattat tatgttggga
     4561 atgatgaaga gcttttgaga aaagccaaac aaaaaggtta tccagataag atgttaaaac
     4621 agattggcta ttacatgcca gaagagagaa tagaaaaaga atcttgtaag gttctttttg
     4681 atagtgaaca gctattagtt ttgttaactc aaaataaatt gatttgtcaa tttgaaaata
     4741 attaatatgg ggtgaagaac catgaatgag aataagatgg tattaagtga atacgcaaga
     4801 ttgagaacca ttaattggtt gaatggtatg caaagtagtg gcgcttttga tggatttgat
     4861 gaggagaata tggactttga aagtttatcg aatgaggata ttagatatta ttttatcgaa
     4921 tggtatttta cagaaccaga tgaagaggtg gaagatgaac cagaattaat tttagaacgt
     4981 caaagaattt tattcagtga acaagatagt aagaggttgt atggtttata tgacttacaa
     5041 ctgaaaggat taaaagcgca gtatgttgct gatagttacg atgatatggt agaacagggg
     5101 gcagataggg ttgtcactta ttcagaagat gatgaagagg aatgggaaaa ctattcagat
     5161 gatgagatat gctttaatga tgggtatatg cctattgaaa tcaccgaaga agaatatgat
     5221 aaagtgtcgg atagcgatga gattggtatg tgtacggttg taagtggatt ggatatagat
     5281 gtccaaagag tagaggttgc ataccagaag atgaaagaac gtataaaaag taaagtagaa
     5341 tcttgatttg attggattgt ttgcaaagta gctaattaag agtataatat aatcataaat
     5401 gtagttaatg gatataataa tgagtatatt atactcaaaa gaggtgaacg aaatgaaggt
     5461 aaaaaatcca aaagaaaaaa gcagaattca agtcggaata gacaaggatt taaaagaaaa
     5521 cgcagaaatg atattggaag agctagggtt aaatccaaca acagcaatca caatccttta
     5581 taagcaagtg gtcgctagag gtgagtttcc agtagagata aagctaagcg aagaggaaaa
     5641 gcaaggaatc agattgcaac agctaactat ggatatgcca gttgatgttc tagatacaga
     5701 tgaaaagtta gaagagtggt tcaatgaagc ctaatgagat agtaacggtc tatgttgcat
     5761 ttacagataa aaatggtggt aaaagaagac ctattttggt ggttagtgat aaagaagata
     5821 gagtagaatt ttttggaatc acaagtcaat atgagaataa atcagaccaa ataaagagag
     5881 tgtattttcc tattgcagaa tgggaaaaag taggtctaaa gaagcaatca tggattgacg
     5941 tagggtcttt aaaggctatt cccaaaagcc gagagaacat atcatttaaa aaggttggta
     6001 tgctatcagc tattgatacg aaaaagctaa ataggtttat cgagagatta caaagaaaag
     6061 tctaacaagg cttttctttt tttgtttata tgtatattaa gatatacgtt tatataaacg
     6121 tatttatgta tattaaaata cacgtaaata tatacgtgat tataaaaatt aagaaaatat
     6181 taagtattgt taaatagtga taattagtaa tatacgaatt ataagatata ttttatattt
     6241 aatgtatgta aataaataaa atgactgacg gatattgaat agtaataaat aacaataatt
     6301 cagacggata cagtgagtga agtagcttgc tacgaacgaa caggcagaat gaaacggtag
     6361 tttcattaga gcaagtcgct tgcgacgtag cgttaccact gatgtaactg gcaagttaca
     6421 tagagagagt ttgcttgcaa acgaacgtta cccacttgaa tagaaaacaa aaataacata
     6481 ttagtgaatt ttgaatagga gccacaactc aaccaaccgc ttgcggtggg tgagtcccac
     6541 ggaggaaaag acgaacgaac ggtcgcgtag cga
//
LOCUS       DAZKOB010000131         6321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000131 DAZKOB010000000
VERSION     DAZKOB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.133"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG8M6_002647"
     CDS             complement(99..1229)
                     /locus_tag="ACG8M6_002647"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0458242.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG8M6_002648"
     CDS             complement(1878..3080)
                     /locus_tag="ACG8M6_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0458243.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG8M6_002649"
     CDS             complement(3140..3343)
                     /locus_tag="ACG8M6_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0458244.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG8M6_002650"
     CDS             complement(3485..4552)
                     /locus_tag="ACG8M6_002650"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0458245.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG8M6_002651"
     CDS             complement(4610..4717)
                     /locus_tag="ACG8M6_002651"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458246.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG8M6_002652"
     CDS             4880..5692
                     /locus_tag="ACG8M6_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458247.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG8M6_002653"
     CDS             complement(6066..6257)
                     /locus_tag="ACG8M6_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458248.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1879 a         1299 c          996 g         2147 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata a
//
LOCUS       DAZKOB010000132         6066 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000132 DAZKOB010000000
VERSION     DAZKOB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.134"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG8M6_002654"
     CDS             complement(254..541)
                     /locus_tag="ACG8M6_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0458249.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG8M6_002655"
     CDS             complement(561..785)
                     /locus_tag="ACG8M6_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0458250.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACG8M6_002656"
     CDS             complement(1240..1410)
                     /locus_tag="ACG8M6_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458251.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG8M6_002657"
     CDS             complement(1425..1958)
                     /locus_tag="ACG8M6_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0458252.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG8M6_002658"
     CDS             complement(1979..2923)
                     /locus_tag="ACG8M6_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0458253.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACG8M6_002659"
     CDS             3560..3646
                     /locus_tag="ACG8M6_002659"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0458254.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACG8M6_002660"
     CDS             complement(3767..4456)
                     /locus_tag="ACG8M6_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458255.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="ACG8M6_002661"
     CDS             complement(4463..4741)
                     /locus_tag="ACG8M6_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458256.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="ACG8M6_002662"
     CDS             complement(4701..5615)
                     /locus_tag="ACG8M6_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0458257.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="ACG8M6_002663"
     CDS             complement(5597..5977)
                     /locus_tag="ACG8M6_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0458258.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1606 a         1119 c         1021 g         2320 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagc
//
LOCUS       DAZKOB010000133         5916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000133 DAZKOB010000000
VERSION     DAZKOB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.135"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(456..959)
                     /locus_tag="ACG8M6_002664"
     CDS             complement(456..959)
                     /locus_tag="ACG8M6_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0458259.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1318..2343
                     /locus_tag="ACG8M6_002665"
     CDS             1318..2343
                     /locus_tag="ACG8M6_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0458260.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2666..3544
                     /locus_tag="ACG8M6_002666"
     CDS             2666..3544
                     /locus_tag="ACG8M6_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0458261.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4020..4136
                     /locus_tag="ACG8M6_002667"
     CDS             4020..4136
                     /locus_tag="ACG8M6_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0458262.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5064..5402
                     /locus_tag="ACG8M6_002668"
     CDS             5064..5402
                     /locus_tag="ACG8M6_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458263.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5496..5726
                     /locus_tag="ACG8M6_002669"
     CDS             5496..5726
                     /locus_tag="ACG8M6_002669"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0458264.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2005 a          887 c         1085 g         1939 t
ORIGIN      
        1 ctcagatttc tctactactt ctaaaaacta ctgttttttt cctttgaaat gccttcaaaa
       61 agtaggatca ggcagaatat actggcagaa tcaaggagaa gggagcctaa ataaggagaa
      121 aaaccataga aaatataaag taattactta taaaacaagc tttttataga aaaataatat
      181 acctgagtag ggtataaatt aagacggaat tcctttctag attgagatat tgactaggaa
      241 gaatgttaag attttaagcc tttaagctaa aaaaaaaccc tttttgttaa ataaggggaa
      301 tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt agattagaga
      361 tgaaaataga tttttttaaa ccaatgactg aataaaaaga ccgtcataga tacacattgg
      421 aatgtgtcta ttacggtctt tttttacgaa cgattttatt gaagacggac agttcgggtc
      481 aaaaataaaa aagctacgat cagaaataag aataaaatgt atcccaaaat aatggctaga
      541 cggtctaact tatttgtcga tagatgtttg tttagaagtt gataggccca taaacctatc
      601 agtcctcctg ttgtattagt tatcaaatca gtgatgtccg ctacgccaat accgaatagg
      661 aattgaatca attctgccaa gaagcttaca aggaaaataa tccctaattt tttagaaaaa
      721 gaaagtctct tgaaattgac attcaaaagc accccaaaag gaaaaaagac aatcaagtta
      781 taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa agggacgaga
      841 ttgacattgc tgtaagaata agtgagaaat tgtggaagat taaatgatag cttgaataaa
      901 atcaaccata atagaaagaa taaataaatc cctaataaaa attttgatac ataccgcata
      961 gacatctcct ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca taaaaaagta
     1021 tgaagtttaa ggaccaacgt agttatcgta cgaaaattag aagctttttt ttgttgggaa
     1081 tccggtttct ttaacaaagc taagatagcg agttgcttgg aaaagttgct atcttttttc
     1141 tgtcaaaacg actcggaatg ttcgtttttt gttctaattt ctccaagttg tttacttttt
     1201 gatactaagt ttatgaaaaa tcaagaaata gttgttatac taggtaatgc tgacaaacgg
     1261 gattggcatt tcatcttggt tcagcttttt ttccaattac taggaaggag ggatagattg
     1321 ttcaagactt attttcgttt caataaaata caagataatc cttttcgtat catgaatctg
     1381 atgctactta tctttattgt attatggtta ggttacataa atcatgattt tttgatttca
     1441 agtttttcat cattagggat ctttactttt ttctattatc aaaacatacc gatgaaatat
     1501 ttgctgaaac gtatgtttct gatcggttgc ggactgttgg ttgctttcgt tttggggatc
     1561 ttatcgactt atgttttatg gcttgaacca tttgccgtag ctttggttgc tttttccagc
     1621 agattcgtgt tgcgattatt ccatatttct aaaccaggtg gcttgttttt tgcgatgctt
     1681 tctgccatgg gaacgagcat gcagctgcca atcgctcaat taccgatcgt ctcactttat
     1741 ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt actggattca
     1801 cggcctgagc agacaattga aaaagcaact ttaaaagaaa gatttcacga agagccttta
     1861 gtgatcattg attctgtttt ctacagcgct gccttatttc tttccgttta tgtcagtcac
     1921 ggcttgaatc tgcataatcc ttattggttg accttgtcat gtgcatcgat tttgctagca
     1981 gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc tgattggatc gatgggtggg
     2041 ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc agttgcagac catttttctg
     2101 atcacttttc tatatggaat cgcacaattt ctagttgcta gaaattatgc agttgctaat
     2161 atttttctta atccgatggc gttgatgcta tctacactta ttcgtggagc atacttgatt
     2221 tctttgatcg aatatcgttt tttagggatc gtcataggaa gtttcatcgg tttaggtgtt
     2281 gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg ttgttcgtca aaaattagaa
     2341 tagaaggaca aatatatgtg ccaaaagagg ttatgacaag atttttgtca caacctctaa
     2401 ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa attcacacaa aaatgtggaa
     2461 aggaactttc aaaaaaattt tatatttatt ctatcaaaaa attttgttta tgatggtaac
     2521 tgaataaact ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga tattctttct
     2581 tcatagaaag tttaagtttt atagttagtg tacaataaaa gtatgtttat accatatggt
     2641 gtccggataa tcagtcattt aaacaatgag gttcaaagat atgaaaaggg gaaatttgat
     2701 gaagaaaata agcaatgaaa tccaaattct ggcagaaact ttaactcttc ccaaagtcgt
     2761 tatagagcaa atgattgtac aagcaaaaaa catccttgat gaggtgtttg agcaatatag
     2821 tcatgaacta atggatccta ataaagcaaa ggcgactgca aagaaaatag acaaagtcta
     2881 tgaagaggaa ccttttgcag cattagctat ttatctatat gcagcttctc aaagttggaa
     2941 aaaaatttat caaccattga agattccaag aactatctat ttggcaacaa tgaatgcttt
     3001 tactagattt attcatgaac atttccaagt aacaggaagc tatcgctttg accgtggttt
     3061 ttggatttgg cattacacta gtggcttgat ctttcggatt ggtgaattgg agtacgaaat
     3121 gacatatttt ccgaaagggt acacggatct aagattagaa ggaaagcagt gcctttctct
     3181 tcatattcct tccgatgcag atttatctca gaataagata agagatagtt acgaaagtgc
     3241 cattcgtttt tttgaacttt attttcctga ttaccattac caagtgatgt acacagatac
     3301 ttggttacta tcacccaatc ttactaaatg gttgaaaaaa gagtcaaaaa tctgtctttt
     3361 tgcagccgat tatcgattat tatcagttga tgaacaggac gatagtggag tcccttggat
     3421 ctttggacga gtagatgccc aaatacatga ttatccagaa agtacgagtt tacagcgaca
     3481 agcaaaagag caattgcttg caggcgagca cattggctct ggcttaggta ttcttacttc
     3541 ataagtttag tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa gaacactttt
     3601 gtctcacgct ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc
     3661 tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta
     3721 aacgcttctg tcccagcctc ttttttagtg ttctatcgaa cacacagtac gttcaatagg
     3781 taaactagct ataacaatag atattaaagt taacggacaa gagggccttt tttaggtagc
     3841 taaaacgtaa taatgaaaat ttatttccag agataggagt caatcagcta gtctgatcga
     3901 ctgttttaac aagttgactt tcattttaac ttttgatacc atttaaaaca caaatcgtaa
     3961 ttgtttcgat ttgtgttttt tagcatgact acaatcaaag aagaaagtgg gtcgtccata
     4021 tggaaaaaac agatatctca agtgcatacc gccgcttgaa aagcccaaat atcaaaacac
     4081 gaaaacgtgc gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa aggtaagaag
     4141 aaggaaactt aattaaaaaa atagccgaaa aacgtttttc ggctattttc aataatcggt
     4201 ttttgtgtaa ggtttccgaa agaactgctg ttgattttcc atttttaaca tatgataaat
     4261 tggtagaatc tttcttttca aattgatacc attccctaat tccagttata tagttaacta
     4321 cagaaccaca aagcacgtac gtttataaaa gtattatctg tagttatttt tcaattccct
     4381 ttcaacctct tattttgacc agtagtattg atcactttac cttttaacac ctttgttcgt
     4441 ttttgaaaca tttttagtca agaatggaaa cgtaactaca taatcaaagg ttttgcgaaa
     4501 tatttcatga aatatttcat gtagaagcga gcaatacggt tgcaaataac cattatcgtt
     4561 taataagcag ttacgaaagc tcccaatatc tctaacgttc agctcactcc atttcttttc
     4621 ttcaaaaaaa tcagtgattc gtatctcttt ctatttgtcg aaaaagttaa ctttaaaaag
     4681 taacgccgaa aaaatatgca aaaaaataaa aaagcagtta ctattgctag aataacatca
     4741 aggaataata agttttggtt ttcagataat agtcgataag ttgtgtattg gaattttaga
     4801 tacagtaata aagtattttt catgaaattt tgtgagaatc actattacaa gtatctttaa
     4861 taaaattaat ggttaaggag gaacttaaag tagaatgtaa atgaatagtt gtagtcagtc
     4921 gattgtgata ctagactcaa agttgcagga ataagttttt caatttgtga ttggaagata
     4981 aaaaatgaaa aagaagggct tttttttata ctttgttgat tgtagtgttt attttccctc
     5041 tgtttagcca agtaacattt tctatgactc aaccagtttc aagtgaggaa catgataacc
     5101 tttttcgtgc tacagaagag tttaagaaat atcaggaaaa tattgttccg gagactccat
     5161 tagctagtga gccaattact gatattgatg gaaaccaaat aggatggtat atacagtacg
     5221 aagttatggt ggtaaataat gactcaatag taaaaaatac agtaggctta gagctaacac
     5281 cgattgaaaa ggtgagttca ttgttaacgt atatttatac tgatgaaagt gacgagactg
     5341 agatcatttt aacggattac tcaaaaattg ctactgataa aaagtatata ttgtggatgt
     5401 agacactagt gaaatacttt atgatataga aattgatgaa aatttactag atgtagcaca
     5461 agaaatcgat catcaaaagc aggaagctat tcgatatgct ggtagaaaag ggaaaagaaa
     5521 atatttatta cgtgaatgta gcaaaagtta gagaagatga aaatgagtgg aaagaattca
     5581 agagtcgcta ctcaattaac tcgactccga catttacagt ttacagagaa ggatcgatag
     5641 aaaaaactgt tttttggact aaagaaagtg gaatgtcttt agctgaagta gaagagtttt
     5701 tggattatgt tagtatgcaa cagtaaggga gttcttatta tgaaaaattt tgacacatat
     5761 tatgttattg ttttttcgat atctttgctg ttaatattac taactttaat gcagtataaa
     5821 gagtatccag agtattttat tttaactttt gtatatttag taagtagcta cacacttcta
     5881 tataaattca gaagttcttc tagagtttct aaagtt
//
LOCUS       DAZKOB010000134         5859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000134 DAZKOB010000000
VERSION     DAZKOB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.136"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(244..2136)
                     /locus_tag="ACG8M6_002670"
     CDS             complement(244..2136)
                     /locus_tag="ACG8M6_002670"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0458265.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2133..3860)
                     /locus_tag="ACG8M6_002671"
     CDS             complement(2133..3860)
                     /locus_tag="ACG8M6_002671"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0458266.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3876..4526)
                     /locus_tag="ACG8M6_002672"
     CDS             complement(3876..4526)
                     /locus_tag="ACG8M6_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0458267.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4730..5026
                     /locus_tag="ACG8M6_002673"
     CDS             4730..5026
                     /locus_tag="ACG8M6_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0458268.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5198..5818
                     /locus_tag="ACG8M6_002674"
     CDS             5198..5818
                     /locus_tag="ACG8M6_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0458269.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
BASE COUNT         1715 a         1205 c         1066 g         1873 t
ORIGIN      
        1 tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag
       61 gttgtaaaat cagcggtttg acctgagtat tagagataca aaacggaaaa atagcttctc
      121 atattttgcc gttttttgag ctaataccga aggacagctg attgaacacc gtttattcgg
      181 aaaaagggac tgtgacacga cttttgtcac agcccctaaa aaaaataata aatttgtact
      241 attctatgaa gcttcttctt cagaaaactg actgttataa agatctgcat agaatccgtc
      301 tttttccatc aatgtatcgt ggtttcctgt ttctacgatc gaaccatgat tcatcacaat
      361 aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt
      421 ttccaatagg cggttcatcg ctttttgaat cagtatttct gttcgtgtat ctacactaga
      481 tgttgcttca tcaaggatca ggacatctgg atttgctaag aatgcccgcg caattgtgat
      541 tagctgacgc tgcccttgcg aaatattgct tgcatcctcg tttaatacag tgttatatcc
      601 ttctggcaat ttacgaacaa aatcgtctac gtgagcagct ttagcagcct caatgatttg
      661 ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca tagatacttc ctgtaaacag
      721 ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg
      781 ggtatctaca ccatcgtaac gaatactacc tccagaaaca tcgtagaacc gttcaagaag
      841 gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca atagcgacca tctcaccagg
      901 tttgacatcc aagttaaaat cagtcattag caagttatct ggtgtgtagc cgaattgaac
      961 attttcaaaa cgaactttgt atggtgtatc tttttcttct ggaaggccag atttttcatt
     1021 ggacatctct tcttcatcca acacttcaaa tacccgttcg gctgaagcaa ctgttgattg
     1081 gatagtgttc atcaaacttg caagttgagt gattggttga gagaactgtg ttgtatattg
     1141 caagaacgct tgaacatccc ctaatgtcat gttcccattt gcaactttta ctccgcctag
     1201 tacagcgacg aacacatagc ctagattttt aacgaaattc atcaacggca tgatgattgc
     1261 cgaaataaat tgagctttcc aaccagcggc atacagattc tgattttctt tttcaaatac
     1321 ttcctggtct ttttcagtat gattgaatgt tttaacaatg gtatgaccac catatgtttc
     1381 ttctacttgg ttattcaata aacctaaact tttttgctga tctgcgaaaa acacttgtga
     1441 tttcggcgct acgatcatca cgatgatcaa gcttaaaggg atcatcaata acgcgactaa
     1501 cgtcaattgc cagctgattg aaagcatcat cacgatgacc ccaatcaatg tgactgcact
     1561 agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg atgttatcca tatcattgat
     1621 tgctcgagac atgatatccc catttgtatg tgtatcataa taggaaatcg gtacacgatt
     1681 cattttggct tccaaatcat gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat
     1741 aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa tacatcacga ttactgtcgc
     1801 aatgatctgc gcgattttat caaagtctat cggaaatttt gttacttgtt ggccttgttg
     1861 catctgttga gcgccagcca ttacaccttt gaatatttct gtggttgctt tccccaagac
     1921 ctttggtgtt tggatttgga agatcacggc tgctattgct agtacaaata cagcgatgat
     1981 cgcaaccata cgtttagaca tatagccaaa caatcggcgt accgttcccc agaaattttt
     2041 aggcttttca ccttttacac cgagatttct gccaggtcca ccatgtcctg gaccgcctgc
     2101 tggtccatga gagggacgtg tgtttgtttt actcatgcta agtcctcctc tcttagttgt
     2161 gaatgaacga tttcttgata agtttcgttt gttgctaata gttcttcatg tgttcctttg
     2221 ccaaccagtt ttccttcatc tagcacaagg atcatatctg catccattac tgtacttact
     2281 cgttgagcaa ctaaaacggt gatactttct ttcatattcg ttttcaatgc ttggcgaaga
     2341 ttggcatccg ttttgaaatc caacgctgag aaggaatcat caaacacata gatatctgct
     2401 gttttgacta atgcccgtgc aattgccaga cgctgtcttt gtccaccaga gaagtttcct
     2461 cctccttgtt ccacatggct atctaagcca tcttccatct ctgaaacaaa atctttagct
     2521 tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg catccggctt accatactgc
     2581 atattgtctc ggatcgttcc agagaacaga actgcttttt gaggaacaaa gcccattaat
     2641 tctcgcaaat tatattgagt catgtcacta atgtccgttc catttatttg gatcgaacca
     2701 gattcgatgt catataatcg agggatcaaa ttgactaagg tcgttttacc tgaaccagtc
     2761 ccccctatga ttgcgacgat ctcgcccgac ttggcttgga aatcaatatc ttccaatgca
     2821 agtttttctg ctccatgata tcgataattg acatgattga aggccaatgt tgcgtttttt
     2881 ccagcaaaag aaactgtttt aggattttca gggtcatgga taccgatttt ttcatccaac
     2941 acttcattaa tacggtcagc tgaagcttgt gcacgaggca ccatgataaa aatagcggaa
     3001 agcatcataa agctgatcaa gatctgcatc gcataagtca taaatgcaat cagattaccg
     3061 acttccaaag tcatatctgc tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta
     3121 ccgctcatga tcaatgtcat cattggcatc atcaatgcga cgatcgtatt aactttgatt
     3181 gccgtatttg tataatcttt attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta
     3241 tcgaatgctc gaatcactct gacccctgtc aatccttctc gaaataccag gtttaaacgg
     3301 tcagttttct tttgcatact tttgaataat ggaacagcaa agtacatgat tccgccgatc
     3361 aaaataatca ttactgggat gacaactaag aagatttttg tcaactgatg atctttttga
     3421 taagcaagta tgctcgcgcc aatcaacgtg attggtgcat tgatcatcat acgtaaaaac
     3481 atttgtgtaa ccatttggat ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc
     3541 gttccatatt tatcaaacgc atcttttgtc agactctctg attttttata gacatccgta
     3601 cgcaattgct tacccagttt ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca
     3661 gctaagatac tgatcaaaga aaagccgatc atcacaaaac ctgtatgcca aatataatcg
     3721 acatctcctt gcgctacccc tttatcaata atattggagg taagtgtcgg caaatataaa
     3781 tctgccagta cttggatcac catgaaaact gcagctgcaa ttgctgaagt caaggagatc
     3841 cgttttacga tttttagcat ctagttccct tctttctatt tatatactcc gtttttcagc
     3901 caagaaagtt tcaaattgaa ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt
     3961 gaaaataagc gatacgaatg cgcaattgtt gtaaatacag tattcatcac taattggatc
     4021 agtaattcca attcatgatc attttttact ttcagcttcg tccgatcgat caattcaagc
     4081 aactcttttg catcttgttt gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc
     4141 gtatgaggag cgacttttct tgaggcacgg taatccatgt tcataaacaa gttacgataa
     4201 aaagaagcat tctcgccttc caaaatctca taaatcattt tagaaaaata ggattcgaat
     4261 cctttgaata gatcaccatc ggattctttc attagttgta acatatcttt agagctgtca
     4321 cgtctcattg tctcaaagta ataaaaataa agatcctctt tatcttcaaa atactgatag
     4381 aaactgcctc tagggatatc cgctaatttg acgatattag caatcgaagc ttcttttaag
     4441 ggtactctag aaaactcgat tctcgcagcc tcgattagtc ttttttgttt ttctttaggc
     4501 aaatggaaaa atgtctgtgt cggcatatgg atcctccttt ctttcaagat gacacattgt
     4561 catatgacgc tgtgtcatta taacacctcc tacctgtttt gcaagacatt tgcatagttt
     4621 ttttcattat catccgatac attaaaaaag tgataagagg aatccttcca tttgaattaa
     4681 tgagagttgc ccattaaaat agccacatac taaacaaagg atgagataaa tggcccaatc
     4741 aattagtgtc caagacttca ataaattgcc tctcgatgct gacagcatcg ttttagatac
     4801 aagaaatacc ttagattaca caaaaaacca cttaagccac tcgctttcta tccccgctgc
     4861 tatcttgcca agacaattaa aaaatctaga tccaaagaaa ctttattata ttctaagcta
     4921 tacaggcagg cgatcagaag cattagccga tcaacttact gctaatggtt ttcgtgctgt
     4981 ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt gcttgaatat tgcttgttag
     5041 attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc ttttataccc tgtctttttt
     5101 tagttattta ttcttcaacc ttatcaatat gacttgatag ccttttcttt ttctcatagt
     5161 tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa
     5221 aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac
     5281 aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga
     5341 tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga
     5401 attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga
     5461 tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc
     5521 aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac
     5581 agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg
     5641 cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt
     5701 aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc
     5761 agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa
     5821 attgagataa atgcatacaa aagagacaga ggaaaaaat
//
LOCUS       DAZKOB010000135         5826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000135 DAZKOB010000000
VERSION     DAZKOB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.137"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..288)
                     /locus_tag="ACG8M6_002675"
     CDS             complement(43..288)
                     /locus_tag="ACG8M6_002675"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458270.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(282..683)
                     /locus_tag="ACG8M6_002676"
     CDS             complement(282..683)
                     /locus_tag="ACG8M6_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0458271.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(870..2678)
                     /gene="uidA"
                     /locus_tag="ACG8M6_002677"
     CDS             complement(870..2678)
                     /gene="uidA"
                     /locus_tag="ACG8M6_002677"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0458272.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2701..4467)
                     /locus_tag="ACG8M6_002678"
     CDS             complement(2701..4467)
                     /locus_tag="ACG8M6_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0458273.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(4480..5274)
                     /locus_tag="ACG8M6_002679"
     CDS             complement(4480..5274)
                     /locus_tag="ACG8M6_002679"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0458274.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(5284..>5826)
                     /locus_tag="ACG8M6_002680"
     CDS             complement(5284..>5826)
                     /locus_tag="ACG8M6_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0458275.1"
                     /translation="NFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILF
                     LIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFA
                     RKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFL
                     YPLSKDKVLENCELLKQKHK"
BASE COUNT         1864 a         1143 c          712 g         2107 t
ORIGIN      
        1 agattctttt aaacagaaaa agtggaaagg ggattttttt aattagttaa ttttaaattt
       61 taagatcgct tctattaatt taatttctag ctgacttatc atatattcat ctacataggt
      121 aacgaattga cctacctcgt taaatccatt tgtcaaacat aacttagcca tataagaatg
      181 ataatgtttg agtatcatct tgatcgaaat tggatctcct tctagagcag ataagataat
      241 ggatagtgga actaataggt actgccgctt tttagattta tctaccatag gtcatacccc
      301 attttcaatt gttcaactgc cttctttcgt tgataccaga taccacttgt gctcatattg
      361 agaattttac cgatttcagc atcattaaaa cctgcaaaat aataaagtag gataatcttt
      421 cttcttatct ccgataactg atcaattgct gctgataact tttgatctga aattaatatt
      481 tccattccaa ataagaaata gacatcttgt tcatcaaaat gagtttggga atacctgcct
      541 tcatttgaat ctgccaatgg ttcctcatta tcctttatgt tagcgtttcg ttttcgaata
      601 gacttagctt cgtttcgtaa tactttttta cagtaggcat caaattggtg ttgtatagat
      661 atgtcattgt aatgttgttg catgataata tgctcctttt tccccctttc cactaataca
      721 acaatcagaa agcccctttt tttcgaaaaa actagaaaaa aaacataaaa atatttatca
      781 ttctttaaac agaaaatctt tctataaaaa acaaaaaaaa acgattaggc taggctaatc
      841 gttttttgaa cattcttaaa aaatccaaat tattttttat aactgaaatt gggtattgat
      901 gaccatcgtt tctgtagata gtaagcaatc attttaggtt ttctatctct agtaaagatt
      961 cctttcttat ttccttgcac tcttaaaatc ccttgacttg ttgcaaaatc tgcaaaattc
     1021 cacacttgtt caccaacaaa attttcacat gtatctatta cttcgtgatt tgcttcgtaa
     1081 taagccactt gatactcttc tgtaaacatt gttggtactg tatcatgaaa tccagctact
     1141 gtatctgctc catattctgt aaacattatt ggcgtatcag gacatctttc catccatcca
     1201 tctagctctt ttttcaacag atattttgca tgtgccagat cccctcccga aacataccaa
     1261 ccataataac gatttaaaca taaaacatcg acaatatctc ctaccttaca aacatctggc
     1321 gttgatttta gataagtaac gattgttaca ggtcttttct gcggatcaag ttctttagtt
     1381 aattcaacta aaggttcaaa atattcttta gcaccttcgg aatccgaatc tggttcattt
     1441 gcaatagacc acattacaac acttgggtgg ttcttgtcgc gttctatcaa ttccttgatc
     1501 accgttttat gagaattttc tgttcgtatc tctttccacg tattatgttt ttccacatta
     1561 tcatctaata aagtggccat gaaatttaaa tgtaacccta ctgcaggtac ttcatcaata
     1621 actacaatac cttctcgatc tgctaatcgc ataatttctt ctgagtaagg ataatgtgat
     1681 gttctaaaag aattagcacc tatccatttc attaaattaa aatctttaat atttacagcc
     1741 tcattcaaac cacgtccatt aacatacgaa tcctcatgct taccaaatcc tttaaaataa
     1801 aaaggtttat tattaatcaa aaatttgcca ttggtaacct gaacactgcg aataccaaat
     1861 tcttctgtat aggtatcgat cactttatta tcatcaatta aactaatttt catactatat
     1921 aaataagcgt ctagaggttc ccaaaaaatt ggattcttaa gctgaatcat tcctttcttt
     1981 ccttcagctt cagctacaag ttgagcctct tcgtcatata tcgctattaa gatttttccg
     2041 gtccattctg tatcgatatt gaattctaca cttgcatcca aatcagttat tcttgtatta
     2101 atagtaatat cacttatata gtctttcgga gttgtatata acttaacagg tctatgaata
     2161 cctgcataat tgaagaaatc aaaattaact aagttttcct tttttatatc cccattttcc
     2221 tgtactgttt ccttcataat ccccacaggt aaagtactat cgtcaataat attgtttaca
     2281 gcaactgtaa ttcgattatt cccgtctttt acaaaccgat taatttcaga ctcaaatggt
     2341 gtaaacccac ctttgtgttg agtaataaac tttccattca aaaaaacttt tgcttcatgt
     2401 gttgcagatc caaaacgaag aactaatctt tcttctaata atcttttagg aagagtcact
     2461 tctttttcat accacaccca tccaacatga tttctaatgt tctctgtttc aaataaatca
     2521 ttataagaag agggaactga aatcggataa ctctcttttt catttattgg ttctaaagca
     2581 agttcttcct gtaagtcatc atttgtatct aatttaaatc tccagagacc gcttaagtca
     2641 attacttgcc tcgtctctgt cataatggga tacaacatat aaaccactcc tatctaaata
     2701 ttaaataaat gtatccttta aaccacaata agcatctatt ggacttactc ccttaaattc
     2761 agaaagaccc attagtttgt ccaccaatat ttctaaagtt tcttgttttc catcataagt
     2821 attaatataa gtctttactt gaggtacatc agccaaatga aacggacact gtacagatac
     2881 aaaaattgtt ggaatttcat gaatgtaaaa cggtatatcc ggagttcctt tacttgctgg
     2941 ccagacaatt cgttgaactg tgcctggatt aacattagct atattaatga tcaagtcata
     3001 ttttttagtt aaatttttaa ttggtctctt agcagcatat attccagcta tatttttaat
     3061 tttttcttct tgcgataatc ctttcaattc ttcttcaggt gaaatgtatt tttcaacttc
     3121 aaatcctttt gctgacaaaa tctgtttcaa taaatcaatg gcttgaggtc tatttccagc
     3181 aactacttca ccaaacccac cttttacccc atgtacatct actagtaaaa tccttttata
     3241 tttttctggt acaataggcc aaatatcttg tttatcttta actaatgtaa ttgcttggtc
     3301 agccacctct ttaaaaattg atttattttc aattaaacca attttttcca gagcttcttg
     3361 cttaggtaat aaaatttgtt cttttgcttt tttgtgtaat cccaatttag cttttaaccc
     3421 taaaattctt tttaaggcat ctaataaccg atcatcagtg ataattcctt tacgatagcc
     3481 ttccatcata taattaaagt cttcatctgg atcattaaaa aatagaaata gatcacatcc
     3541 agctgcaata gaagtgggca ataattctga tcgtttcatt gccccagtca ttcctaacat
     3601 atgactagca tcagtaacta ctaatccatt aaatccgagt tttcctctaa gtaagtcagt
     3661 gattaattct tttgataaag ttgcaggaag taaatcttca tctttcagat caggattaaa
     3721 atgttttgaa taagctggca tatggatatg acctgccata attgaaggta atccagcttc
     3781 tattaagcct gagtatactt ttccaaaaga attatcccat tcttccgtac ctaaactatt
     3841 aatactaaaa gatagatgct gatctcgttc gtcaataccg tcaccaggga aatgtttagc
     3901 tgcaggcaaa attccactct cctgaatacc tttcatataa gctaaactaa gttctaaaac
     3961 tttatcaggg ttttgtccaa aagatcgact agaaatgata ggatttctcc aatttctatt
     4021 aatatctaca ataggagcaa aactccagtt acatccaata gccgatgctt caatcccaga
     4081 aactctccct aattcataag catatttcgc atcatttgtt gcaccaattt ttacttctac
     4141 accaacttca gtaccatctg tacaagctcc gttaccacct gcttccgtat tagcagcaat
     4201 taatagagga attttacttt ctgtttgtaa gatatagttt tgatcatata cctcatcagc
     4261 agttcctgga ttatatcgta cagctccaat atggtactga ttgaccatat tactcaagta
     4321 atttttatca cgactagatc ccatattaac aaacaattga cctatttttt cttccaaagt
     4381 catagaatca atcgtctctt caatccaaga aatagcatct tcatctaaat tatatggttt
     4441 ttttcgtaaa tctactttta tagtcatttt ctttctcctc tacttattag taagattttt
     4501 ccaaaaagta tctatatatt tttgttcaat atctacataa tctcccgata aacatttaaa
     4561 taaaacatca gttcttaagt tttcccatga ttctttttct ttattgacca aaataggata
     4621 aaagaaaact ttatttttat ttgcagcttc tagatcacca ggagaatcac caatcattaa
     4681 tatcttatcc aaatcacaac cattttcaat tacacttcga ataatatctt ctttttttcc
     4741 tttatcttga caacacagtt catcaactaa tgataataat tcatgtcttt cccattcttc
     4801 ttgaacagct tccttattag ctgagcttac aataattatc ttcccatatt ttttcagagc
     4861 ttttatagta tctctagcac ttaaaaaggg tttatctaat ccttttgctt gactaatctg
     4921 ttgattgact tcttccgacc attttaaagc ttttaataaa tcctctgact gagaatattc
     4981 tatttccctc ttcaaagatg gattagacaa ttcttttgtt agagataccc agtgctttaa
     5041 tcgattaatc gatgttacat catttttttc taatgtttta actaaaccta aaaatctgtt
     5101 tattccacga gtctctgaat ataaattaat attattccaa tcctgtagga atttttctct
     5161 atgatgaaca tcaaaaatct ctgcagcaat tggtccgaaa aagagattgt gtttataagt
     5221 catcgtatcc ataacacagc catctgaatc aatacaaaat atccaattat tcatatttct
     5281 ctcctattta tgtttttgtt ttaaaagctc gcaattttct aagactttat ctttagataa
     5341 tggataaagg aaaaataaaa ttaatcctac aatcagaaaa caaattgatg gaattaatgt
     5401 tgaagcgtta tataactttt ctaaaacatc tcccgtttgt acatttgcta aagagtcata
     5461 gcctataata gaaagagtcc aacctccgac gcctccagct agagcttgac ctatttttct
     5521 agcaaaagaa tataccgcat atacggtccc atcttctctt tgatcagact ttacttcttg
     5581 atcatcaata acatcagtaa tatttgccca aataacagta ttaaacacac ccaaacccat
     5641 atagccaacc gaactaagaa ttaagaatac atacatatta ctagctttta tcaaaaataa
     5701 gatcaaatat actattgctg aaaagaacat tcctactgat gccagttctt tttttccata
     5761 ttttttactt aaaaacaaag ttaaaggcga aacaactatt aaaaccaaaa taggattgat
     5821 aaaatt
//
LOCUS       DAZKOB010000136         5758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000136 DAZKOB010000000
VERSION     DAZKOB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.138"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(185..487)
                     /locus_tag="ACG8M6_002681"
                     /pseudo
     CDS             complement(185..487)
                     /locus_tag="ACG8M6_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            580..929
                     /locus_tag="ACG8M6_002682"
                     /pseudo
     CDS             580..929
                     /locus_tag="ACG8M6_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1201..1491)
                     /locus_tag="ACG8M6_002683"
     CDS             complement(1201..1491)
                     /locus_tag="ACG8M6_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458276.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1893..2129
                     /locus_tag="ACG8M6_002684"
     CDS             1893..2129
                     /locus_tag="ACG8M6_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458277.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            2136..4088
                     /locus_tag="ACG8M6_002685"
     CDS             2136..4088
                     /locus_tag="ACG8M6_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0458278.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4160..4657)
                     /gene="dfrG"
                     /locus_tag="ACG8M6_002686"
     CDS             complement(4160..4657)
                     /gene="dfrG"
                     /locus_tag="ACG8M6_002686"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0458279.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4984..5676)
                     /locus_tag="ACG8M6_002687"
     CDS             complement(4984..5676)
                     /locus_tag="ACG8M6_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0458280.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1866 a         1001 c          932 g         1959 t
ORIGIN      
        1 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
       61 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      121 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      181 tctcaacata cttgcaaagt aatactacaa ttcctaacat aatcgtagaa tttaataatt
      241 tccatagcat aaagtgttta gaaaagaaca ttaagaatag ttctataata attcttgaac
      301 tccatgtctt ataatgttct atcgaagcac ccaaatagcc aagttcatct aatctacttg
      361 cataaactac gtcatccgct ctcatagtcg gcatatacat attaaagagt aaaaagatac
      421 cccaaatccc tattagtaac aagatcagtt ctttgttatt cgttattttt ctattatgtt
      481 tgttcatttt tatactcctt cttatatttc ctaatagagt ataacatagc tttaagcatt
      541 acttaaagat aacttaaaag ttatctttta aatacttaat tgttaaatac cttatttgat
      601 gcaaaagaaa tctacgatag tcaacaaaaa aattttcatt atatccagaa aaatttttct
      661 tagttaagga cctatggatt gcagtaatgc agaattcgtc gaataaactg ccaaagacat
      721 acaatcatat tgcttttaaa attgacgaac aagatataga ttcgtttgtc tcaaaaatcc
      781 aaatgttagg tttaactgtc gaaccaggac gttctcgtgt gaaaggcgag gcttcttcaa
      841 tttactttta cgactatgat aatcacctct tcgagttaca taccggtact ctccaggaac
      901 gtttaaaatc ctacaattcc actacctgag actagtaatg actagtctct ttttatggcc
      961 tctgatcccc ccattgagcg aatactaata gggcttcgaa ttgatctgtt caatctttaa
     1021 gcaatatccc tcaaatgagt ttggaaagtt tagtgattca tcacaaatac ttaaatacat
     1081 gtagttatat atagattgag gttcttgtga gcgtatgctc gtgtgggttc aaatcccatc
     1141 gtctgcatcc atctttccaa aacaagcggc taggacattt ttgtctcagc cgcttgtttt
     1201 ctagtttatt ctttcatctt gtcggacctt ttttcttggg tatagacttt tctcatattc
     1261 taccacaatt ttgggtttcc ctaacacatg ataggatctc acaacaaatt gctttctatt
     1321 attataataa cctgtaacga ctaacttcat cccttcgtct acttcagcta ggaagttcaa
     1381 actatgggaa gcaatcaaac aattgacccc atttaaactg aaacggatca acggtctggc
     1441 actaacttgc aaagtacgga tcttatctac tgttccatat tttttctcca taaccttcac
     1501 ctcaatacca ttatacgaac atacattccc tttgtaaaga agaaactctg ttttatcttt
     1561 ttgacttttt gtctattacc tttcttaaaa tcatattggc acttcctgcg aagatgaagg
     1621 acatgtacta aaaaaaagag gcagcagtat agatttaagt agttcagttt tggagtacaa
     1681 aaagtgaaaa tgtaaaccta aatgagtatc aaattgcaac tgaatttgat actcgttttt
     1741 tattgttaaa tcaacgtttg ttgttaattt acaccacaaa atgtaagtta aaatgtatat
     1801 aaaaatgcga acgtgaaata attattgtag gaatgcaaat tggtgtgtta aaatggttac
     1861 aatttactaa gcaaaaaggg gtgcattttc ctatgcaatt tcttgagcca aaaaataaga
     1921 acgctaagtc tgtggattgg gaaatatcag agcaagtaag ggtgatagtt aagcaatacg
     1981 ctgaatatgc agagcgtact gaaagcgaag cagtggatga attcttattg aacattttag
     2041 acgataagaa gtttattgaa tggattgcca acaagcgaag taataagcgg attgttgaaa
     2101 aaatgggtat caaagataga gtgggttgat agacaatgac caagcttaaa agattagcca
     2161 cgaaggaaga tgaaattgta gatgttaaaa ttccgatttc aaacgaagaa ttaaaagata
     2221 gggctaaaca atatgattta ttaactccaa aacgatttgc aacaaggtat aacaaaatgt
     2281 tgttcttgcc aacgtctttt aaatggaacg gttctgaata tcccattcaa tataattact
     2341 gcatcaatcc gttttgttgt aattttggga aagaacagca caaattcaaa gatgtaaaag
     2401 ggaaaccaag tcgatataaa atgactggta gttccaaaga taaaggtcat aaaggtatgt
     2461 actgcaatga caatcctatt ggcagaggtg tatcgcaaaa ctgtacagta acccctttat
     2521 ctaattggtc tgttgttgaa gaaattaaac gccttataga aatcaatagc atccaagatg
     2581 ttgagccaga ttatcaattt cataaagaag gctgctcgga agaagaatca accccattca
     2641 atgagccgaa gcaattttac aaaaggggga aaagtagggg caaatctcaa cgctatcaat
     2701 gtaaggcgtg taagaagttc actaatgttc taccaaaaag agaagaaacc actacttatc
     2761 accaacaaaa gaacaccatt cttccaatgt tcgctaagat ggttgttgga agggtttcag
     2821 tcagtcgtac ttgtgatatt ttaggaattg gagttggaac ctactatcat aagctggagt
     2881 ggctgtatag gcgttgtttg gagtttttag aacgatatga gacacaaccc cttcaaacaa
     2941 agaaatttaa cgagatgtgg cttaatacgg acaagatgca ctactaccta aataacgtcc
     3001 gtaagaaagg acaaggttca aagaaatata cggggtttga agaccttaat atgcaaacgt
     3061 atatagttgt atctgctgag gtcttatcaa gatatgtgtt ccgttctgat gttgcctatg
     3121 attggaatat ttcaatggat gaattaaatg aagatacaag aaaattcaaa gaagaccatc
     3181 tcaatacttt tagtcgtaaa aatgataggt tagattggtc ttattatcca caagaaccat
     3241 ccgctaacga tagcgaaaat aggaatgctt atcttcacga attagggaaa attactaata
     3301 ggagtagatt tgtcgatggt ttaaatgttg atgctcctta tactacaact gctcactatt
     3361 ggttaattaa acaaatggtt aatgccgacg aatggcgaat gattagtgat gatgacttct
     3421 ctattagaaa cgccttttat agagtattca cgaaagaatt aaggttgagc gatgcacatc
     3481 attttatttg ccaagttaac aagacgaaaa gcagaaaaca atgcttaaaa gaattcggac
     3541 aagcaaaagc agaactgctc gattggggag atatcagagg attcaaaaca aagtttctac
     3601 ggactttggc atctcattat ctaactgaat tactaacaag tcatcaattc cacgaagagg
     3661 ctattagtaa agatggagaa agatatcgca aatatgccga taaccctatc aaacatccat
     3721 tagccactaa ggataaagga ttttattcag ttgattgtag aacggattta tctgctctgg
     3781 aaccaaacga aatcgcaaag atgctattaa atgttaatga ccattcgacc aatagcttta
     3841 ttcaacagat aagacgttat atatcatctc ttgaaagacc attaacaaca gctcgtggtg
     3901 ataagaagag ctatatttac gctaacttca atccgaagta tgctcaattt gcgattacaa
     3961 ttctaagaac ttactacaac ttttgtcgcc cttttaaatc agcggataaa aaggttttga
     4021 cacctgctca aagattaggg attacagata aacaatttga ttggaaagat ataatttatt
     4081 tcaaatgaca aaaagagctt ggagtaatcc caagctcttg tgctttatct ttactgttct
     4141 tcttcaatta acgcacccgt taactcaata agttttttct ttcatataca tgaaaatagt
     4201 agttatacgg atttttatca ttctttatcc ctttttgggc aaatacctca ttccattcct
     4261 cataattcac ttctggaaaa aaagtatctc cttcgaattc atgatgtatt tttgtgatgt
     4321 acattttctc aacataaggg aaaaacaaat tataaatctg ttctcctccg aaaataaaaa
     4381 tttcttcttc gtttttacat aactcaaaaa catcttctat tgaatgaaca atttcacaac
     4441 cattaaaggt aaacccctta tctctcgtca gaataatatt tcttctgtca ggtaaggctc
     4501 ttccgattga ttcaaggttc ttcctaccta atattatcgg atgtcccttt gtagtatttt
     4561 taacatattc ccagtccttg ggaatcctcc aaggaatgtc attctctttg ccaatcactc
     4621 tattcttatc catcgcagca atcaaagaaa ctttcattta taacacagcc tttttaacta
     4681 atttaccatc taatttctta tttctatcaa aaaacctttt ctcattatcc tgcccgttag
     4741 ttgaataagc aattttttct ataagttata ttatcataat accacaaaat cacaaatttt
     4801 cattcaatta tcattttcaa ttgcatttta taaatcattt tgtatgtaat ttggtatgta
     4861 agttaaaatg aaaaaagtag tccattttga agtacatttt gtcattttgt actccaaaac
     4921 tgaactactt agtatagatt actgttgctt ctttactaat ttaggtttac ttgttaagaa
     4981 agattattta cgcaaatgtt cttcaacaat ttgctgagat ttatcagctg tatcttccac
     5041 ttcatctttt gctttttcta acttttgaat atattgtttc aagcttgagt tttcagcagt
     5101 tgcttcgtct aatttctttt gtgtatctgt taactgagtt tctacatttg ttttttcttg
     5161 tgtcagtgtt gcgatttgtt gattcaaacc atttacagta ttttgtaatt ctgaaagttt
     5221 tgcttgtgct tcttgtagtc ttgctgtcca atctgcattc agatgattga tttcatcatt
     5281 tttttggttg atctcatcta gtttgacatt gatttcattt tgtttgcttt gtagttgtgc
     5341 atccttgctg actaattgac tttctaagtt ttgtttttct gctgccgatt gatttttttg
     5401 gttttctaat tcatttactt ttgtttggta ttcttggaca tctgcctttg aatcttgcaa
     5461 tgtctgttct ttttgtgcta gtcgagcggt aagatctgta atcgcttgct ctttatctgt
     5521 caatgtatga tcaagctgtg tcagattctc attgattgta tcaatatctg tctctccacc
     5581 ccacaaaata atttcatttg cgtaccgcgt gcctgtgtag ccaattagac atagaaatgc
     5641 aacgacaatc ccaccagtaa taaatttctt tttcatttgc atagctcctt aatccaatac
     5701 ttttacatgc taaaagtaag tgatctcaaa cacaatgtca agcgttttta aattacat
//
LOCUS       DAZKOB010000137         5749 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000137 DAZKOB010000000
VERSION     DAZKOB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.139"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..500
                     /locus_tag="ACG8M6_002688"
     CDS             129..500
                     /locus_tag="ACG8M6_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458281.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            585..785
                     /locus_tag="ACG8M6_002689"
     CDS             585..785
                     /locus_tag="ACG8M6_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458282.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            919..2145
                     /locus_tag="ACG8M6_002690"
     CDS             919..2145
                     /locus_tag="ACG8M6_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458283.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            2742..3137
                     /locus_tag="ACG8M6_002691"
                     /pseudo
     CDS             2742..3137
                     /locus_tag="ACG8M6_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            3215..4254
                     /locus_tag="ACG8M6_002692"
     CDS             join(3215..3712,3715..4254)
                     /locus_tag="ACG8M6_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFQ0458284.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            4267..5205
                     /locus_tag="ACG8M6_002693"
                     /pseudo
     CDS             4267..5205
                     /locus_tag="ACG8M6_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            5198..5545
                     /locus_tag="ACG8M6_002694"
     CDS             5198..5545
                     /locus_tag="ACG8M6_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458285.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            5542..5724
                     /locus_tag="ACG8M6_002695"
     CDS             5542..5724
                     /locus_tag="ACG8M6_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458286.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
BASE COUNT         2133 a          829 c         1133 g         1654 t
ORIGIN      
        1 aagatatttt atttggataa tcatgtgaat atgaacttaa caattaaacg tatttacgat
       61 cttttatcac ttatataaag agagaagtga taaataggac acaaaatttg gaaagtaggt
      121 aagtcactat gagagaacta actttatctt ctaaggaatt agaaaagaaa actgctttat
      181 taaacgaaat atttagtgct gattgtataa tccaaagttt aaaagaaagt tcattttcaa
      241 aactaacaga tcaggaaaaa atttcttata tgctaagttt agataatcta ggaactaaat
      301 atgatgaatc agcaagtagc gataagcttt tgattgaatt agtatcaagt cttagtagtt
      361 ttggaaaaat gaaaagattt aatgtgatat tagaaatttt tgcttggtta atcccttcat
      421 ttatttggtt tttttatatt ttcatagttg aatctgagtt aatagccaag gtctctcttg
      481 gactgttaat tccgatataa ataggttcag caatttactt taataacttg tatacaccta
      541 ttcaaataaa aaagcagtta ttacatgaag gattaagtgg taatatgtta aaaagaattg
      601 catgggataa agataataaa acagatattg agcttaaaga atataaatac aattatttct
      661 cgagatgtat agatagaaag ttactgttta aaaaagaagg attagaattt agttttgatg
      721 aagatgaatt tgaaaaatta gtgatggact ggcgtaagaa tttactggat acggtacaat
      781 tctaaaagca attgaggtcg aaattccgat acttgaatca tgatctatat tatcgtgtta
      841 tgagtgaaat ttctaataaa gataagatat ttcatttgga cagtcatgtg aatatgagct
      901 taacaattaa acgtatttat gatcttttat atgtttataa acattaatga ggtgctaaaa
      961 ttgacaacaa taactatgac aaaaaaaata gaagtatatg ttgatggatc acaagaagaa
     1021 agggattcct tttttgattt tttttggaaa aatagtagag aatatgtaaa agcgttgaat
     1081 ctggtatata aacagctaat gtttgaagag ttgataaaag atgcaattaa agaaatggat
     1141 caaggttatg ctgagagaga agaaagtatt caaaatcgga ttgaaaagtt agcatatact
     1201 gatccaatag atcaaaaaaa acttgataaa gcaaaagctg aattatctaa attgagaaat
     1261 gaaaaaaata aagaagcgag agaaatatta acgcaagcga tagggttgaa gagtcaaacg
     1321 agaacacgag atgtagcaaa aaaattagac tttgactttg gagattttgt tgattccgca
     1381 aatatgaaag ctagtaaaga ttttggcaat gattttatcg gaattgtgac cggacaaaga
     1441 acgcctagaa tatataaaaa tagaaacaaa tatggattat taccttttag aggtcgagat
     1501 attaaaatcc ataaagatga taagttttat atttatttac ctaaaggctt taggtttaaa
     1561 gtgaatttag gtagcaagcc aagaaaagca agagatgttg cttcagtttt acaacacatt
     1621 atggatggcg attacaaatt aaatcaaagt aatcttattt ttgatggaag aaaattattt
     1681 tttaatctaa caatttcttt tgaaaaaagc tataatgaaa tggaattcaa gaaaggtaat
     1741 gaattgatcg taaactttgc agatttgtgt agcggtgaat gtttaattaa tgataaatct
     1801 gtaatgtctt ttggtgatag ccaatatata agaaacttca aaagtaaaat tttagcaatg
     1861 aaatcaaaag aacaaagccg aggagtcttt tctaagggcg gtcatggtcg aaaaagaaaa
     1921 ttagatcaag atcgatggga agcaataaag aaaagagaaa gtaattttgt taaaacttat
     1981 aataatcaat gttctgcaaa aatcgttaaa actgctattc gcaataaagc tcaaattatt
     2041 cgtatcattg aaccagataa ggcttatggc gaatgggctt actatcaatt gaaagaacaa
     2101 ctagtaaaca aaggaaaaag atatgggata gaagtcagaa gttagtaaat taaacaatcg
     2161 ttgatttggc aacgaaatag tgagggaaaa tccactcact gaagggtaaa ctccgtcaag
     2221 ttgtgtgacc agacggaagg acgaatgttg ttgaggtttt agaaccatat aaaacagcat
     2281 gtttctcaaa tttttaggat aagggaaatt gctagcagtt tgttttagaa ccatataaaa
     2341 cagcatgttt ctcaaatgag tactttaaat taattgatgg cgttcctgct ttagaaccat
     2401 ataaaacaac atggatttaa aacaagaagt aagtcagttc tccttttact ttagtaagga
     2461 aaaataaagt attctcaata aaacttaaaa taagcaaacc tttgttttaa aatgattgtc
     2521 aagttaaaaa aatctgggat catataaaat agtatagttc tcaaatatga ttagaaaccc
     2581 tattaaagag caatattaag ccaacttact taaatcgttt aattaagaac aataatctct
     2641 tttagtcaga cttttagtct gacttttttg tatttccaaa atttattctt tacaaaaacg
     2701 aacatacgtt tgtataatta cttgtgaatg ggggaataaa aatgtttgat tacagtagag
     2761 aaccggtgaa tgactatttt ttaatcgaca tgaagtcttt ctatgctagc gtggaatgta
     2821 ttgaacgaaa tttagaccca atgactacac cgttaattgt catgagtaga tcggacaaca
     2881 ctggttcagg tctgactcta gcttcttcac ctactgcaaa gaaaaaatat catattacta
     2941 atgttagtag agctactgat ttaccttatc cattaccaaa agaacttcat attgttccac
     3001 cacgaatgaa cttatatata aaaaaaaata tggaagttaa tcagattttt agaaagtatg
     3061 tcgcagatga agatttattg atttattcga ttgatgaatc aattttaaaa gtgacaaaat
     3121 ccttaaattt atttactata taaattgagt taaattgtat tcgtttttcc tcctataggc
     3181 gtatactaaa gaaaacactt gaggagtggg gaacatggaa acagaacgcc aaatccgtga
     3241 attggaaaaa gcgatgaata cgacagacaa ccgccggttg tacgagcgtt tcctggcagt
     3301 caagttggtt cttgaaggcc acacccggaa agaagccggg cacaccatcg gccgcgacga
     3361 gcatactgtc agccactatg tgaaaaacta ctgccataat ggaatccaag gtttagtctc
     3421 caaaaaacaa agcggacgac cttcacggct gacagatgaa caggaagcgg aactggtcca
     3481 aatcgtcgcc taccacactc cggaggaagt aggtttcaaa tcccgtgcca attggacatt
     3541 ggcgattgtg tgtgacctga ttctccggga atggagttat ggatatacgc aaaaaggcgt
     3601 ggcggacctc cttcaccggc taggactaag ctggacccgt ccgacgtata ccctcaaaaa
     3661 agcggattcc aagaaacaac aggtattttt ggaagaaacc ttcccgagcc taaaaaaaaa
     3721 ttgctgaata gcgatattga ccacctccta ttcgaggacg aaaccatgat ccgggattac
     3781 caagccattg gctgcacctg gtttctccgc ggccaccagc gaaaaatccc cacttatgga
     3841 caacacaaag gggtaaaatt gataggcacc ttggattatg aaaccggtcg gatcatttgt
     3901 acagaagaag gggcatacga tgcagcggcc ttcctccgtt ttctggagcg gacgttgcgg
     3961 gaatacccca ccggaaaaat cgtgatggta ttggataatg cccttatcca ccacgccaag
     4021 ctgatccagt cttttcttga agaaaacgct aggcgcctgg cactcatctt cctccctccc
     4081 tacagccctc agttgaacct aatggaaggc gtttggaagt ggctcaagga atcggtcatc
     4141 aacaatgttt tttttgacca cgtccagaaa atcaaacagt cggtccgcgg tttcttagcc
     4201 gatgtaaacg agcgtcccct tgaggtgatt gaccgactgt gcgtgcggat gtaaagtatt
     4261 taattcaacc tatatagttc aggaacaaga agcgagagaa gaagagaatt agctaaaata
     4321 atcaaagatg agattcagaa agaaacaggt ctaatagcaa cagtcggaat aggagataac
     4381 ccattattag ctaaacttgc actggacaat gaagcgaaga gtagtaaaga atttatagct
     4441 gagtggactt atgagactgt acctgaaaaa atatggaaaa taaacgagat gactgacttt
     4501 tggggcattg gatcacgcat gaaaaaaaga ttgaatcata tgggaattat gagtatagaa
     4561 gatttagcaa attggaatcc ctacacaatt aaaagtcagt taggtgtaat cggactccag
     4621 ttatatttcc ataccaatgg aattgatcgt acggatattg ctattccacc agaaccaacc
     4681 aaagaaaagt cttatgggaa ttcacaagtt ctaccaaaag attatacgag gagagaagaa
     4741 gtagaactag tagtcaagga gatggcagag caagtagcca ttcgaattag acaacacaat
     4801 tgcaaaactg gttgtgtacg cttagcaatt ggtacgtcaa ttttggaaga aaaatcaggt
     4861 ttctcgcacc agatgaaaat accaattaca gataatacga aagaattaca aaattattgt
     4921 ctttttttat tcaataagtt ttatgagggg caggaagtac gacatatagg agtaacttat
     4981 agcagattag tatacacaga ttccctacaa ttagatttat ttagtgatcc aagaaaaaag
     5041 attgctgaag aaaatttgga taggattatt gataagatta gacaaaaata tggatttaca
     5101 gctattatac atgcttcatc tatgttagaa ggtgcaagaa gtattacgag aagcacttta
     5161 ataggaggac acgcaggagg attaggaggt ttgcataatg attgaatata acgatagagg
     5221 acgtttaaaa tggtcgggat tttatttgtc tgatcatact gaaagaatca atgcagacaa
     5281 tgtacaacgt gctaatcata atttggcaaa agaacaaatg acgactgaag agattgcgaa
     5341 aatactgaga actgctgtca taaaaaatag acccgtctgc attcaaaaag aggaacgaaa
     5401 cgctgaaggt tactatccag atgatgtagt aggaaaaatt attggatatg acgagcttgg
     5461 tgtatttgtg gaagaagaaa aagttcacta tgatgaaatt aggaatgttt ctttctcgga
     5521 attattaaaa tggagtcaga tatgaacatt attaaaaaat acgaattcgg ttatattacg
     5581 tatgaacagc taatagagga attatgggga tatggtcaac aattaattaa tgaagtgggg
     5641 attgattgct tttgtttcta tattgaatcc ggagcaggat accataagaa cagatactat
     5701 atatttccat atccaccaaa atagtctctt agatattttt aaattttct
//
LOCUS       DAZKOB010000138         5689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000138 DAZKOB010000000
VERSION     DAZKOB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.140"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG8M6_002696"
     CDS             complement(488..1297)
                     /locus_tag="ACG8M6_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0458287.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG8M6_002697"
     CDS             complement(1297..2043)
                     /locus_tag="ACG8M6_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0458288.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG8M6_002698"
     CDS             complement(2061..2528)
                     /locus_tag="ACG8M6_002698"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0458289.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG8M6_002699"
     CDS             complement(2528..2938)
                     /locus_tag="ACG8M6_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0458290.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG8M6_002700"
     CDS             complement(2949..3962)
                     /locus_tag="ACG8M6_002700"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0458291.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG8M6_002701"
     CDS             4179..4913
                     /locus_tag="ACG8M6_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0458292.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACG8M6_002702"
     CDS             4897..5607
                     /locus_tag="ACG8M6_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0458293.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAZKOB010000139         5481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000139 DAZKOB010000000
VERSION     DAZKOB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.142"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8M6_002703"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG8M6_002703"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACG8M6_002704"
     CDS             complement(580..1353)
                     /locus_tag="ACG8M6_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0458294.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACG8M6_002705"
     CDS             1497..1847
                     /locus_tag="ACG8M6_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0458295.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACG8M6_002706"
     CDS             1828..2544
                     /locus_tag="ACG8M6_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0458296.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACG8M6_002707"
     CDS             2544..3992
                     /locus_tag="ACG8M6_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0458297.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACG8M6_002708"
     CDS             complement(4062..4571)
                     /locus_tag="ACG8M6_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458298.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACG8M6_002709"
     CDS             complement(4561..5286)
                     /locus_tag="ACG8M6_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458299.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAZKOB010000140         5189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000140 DAZKOB010000000
VERSION     DAZKOB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.143"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG8M6_002710"
     CDS             137..748
                     /locus_tag="ACG8M6_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0458300.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG8M6_002711"
     CDS             821..1750
                     /locus_tag="ACG8M6_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0458301.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG8M6_002712"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG8M6_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACG8M6_002713"
     CDS             complement(2693..3895)
                     /locus_tag="ACG8M6_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0458302.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACG8M6_002714"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACG8M6_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5189)
                     /locus_tag="ACG8M6_002715"
     CDS             complement(4706..>5189)
                     /locus_tag="ACG8M6_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0458303.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAZKOB010000141         5176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000141 DAZKOB010000000
VERSION     DAZKOB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.144"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1098)
                     /locus_tag="ACG8M6_002716"
     CDS             complement(136..1098)
                     /locus_tag="ACG8M6_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0458304.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACG8M6_002717"
     CDS             complement(1100..2539)
                     /locus_tag="ACG8M6_002717"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0458305.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACG8M6_002718"
     CDS             2724..4670
                     /locus_tag="ACG8M6_002718"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0458306.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="ACG8M6_002719"
     CDS             4934..>5176
                     /locus_tag="ACG8M6_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0458307.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DAZKOB010000142         5090 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000142 DAZKOB010000000
VERSION     DAZKOB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.145"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1050)
                     /locus_tag="ACG8M6_002720"
     CDS             complement(139..1050)
                     /locus_tag="ACG8M6_002720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0458308.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1427..2065)
                     /locus_tag="ACG8M6_002721"
     CDS             complement(1427..2065)
                     /locus_tag="ACG8M6_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0458309.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2069..4051)
                     /locus_tag="ACG8M6_002722"
                     /pseudo
     CDS             complement(2069..4051)
                     /locus_tag="ACG8M6_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4231..4896
                     /locus_tag="ACG8M6_002723"
     CDS             4231..4896
                     /locus_tag="ACG8M6_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458310.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
BASE COUNT         1636 a          923 c          780 g         1751 t
ORIGIN      
        1 ttctaagtaa aaaaaatttt taaaataaaa agaactaaat caagtaataa ttaaactact
       61 gttgatttag ttctaataaa aagatttaga tgaacagttt tattgattta aatagttcat
      121 attttttatt tataccgctt atttactact tgctgtacta gaatctgaag tgaatgtact
      181 caaaacattt tcaaagtctt tgtctttgat ttttacatta gcgtctttca atacttcgcc
      241 aattacttta ttctggaagg tactgtcact taattttgtg tctgttgcaa tttctttcaa
      301 ttcttttttg tatttatcca tgtcgttgcc tttgttttgg tttttcacca ttttaaccac
      361 ataatattcc gtagtataag ttgaagcatt ggtagaagtg atcacatcag aaacttgacc
      421 atctttcaac ttaaaggctg cttctttgac ttctgccgga accgttgtag tagtggaatc
      481 aaatttcact tttccgccat cttcttttgt cgtagtatca gttgacttgt ctttggctaa
      541 tttagagaaa tcgtctccct tatcagcagc tttcttcaca tcttttgcat catcttcact
      601 tgctacttgg ataatttgag cttccacttc tggatggaaa gattcccaag ccgtttttaa
      661 atcttcatcg gtcaatttga tatgtttctt tagaccttct tgaaaggcta gactttgttt
      721 gatactgtct ttgaatgttt gttctgtgta acctgcagat ttcaattgtg aatcaaatga
      781 atcacccaat tgtttctttg tttgatcata ttgggaatcg atttctttgg ttgatacgtc
      841 atctccatat ttatcttcaa atacttgata aacgatcaac tgttgcacag cggaatctgc
      901 tccttgcatt tttttcaagc tatccattac ttcttgttcg ctgattgtac cgcctttata
      961 actaacggct gttttttcac cggcattcgt acagcctgac aataagccag tgcctagtac
     1021 gatggcaagt gctaaaaatc tttttttcat ttatcaattc tcctactttt cgttttttgc
     1081 gaattgctaa aaacaaaggt gttcaagatg aaaatcatca agaacacctt tactaatctg
     1141 ctcgatacgt cctgaataga tataaggcta cacaatccag aaagttcgct cagcagaata
     1201 aatgtcaagt agcacatcac accttaatat actacttctt ttgttaccgt ttttcaagtc
     1261 tatcttgcgt tccgtattta acgtaactaa ttcttagaac aaatgttatc gattttgttt
     1321 ttcttcttcc gctgttggga tattcaaatc agacactttt atgttcgatt ttccagacat
     1381 ttttttgttg tttttattag aaaaagtttg ttttttcatt tgttttctaa agcatgatct
     1441 tcttgtttcc tattttggtc agatatggat catgcgtgac cacgataact aacgtacctt
     1501 gggaagcctg ttgcgataag atttcaacga cttgctcctt atttcgttta tctaatgatc
     1561 cagttggttc atcggctaaa atgattttgc tattcttcaa taagactctc gcacaggcga
     1621 ttctttgctt ctcaccacca gataatgtgt agataggcgt acttagcttc ttgtttatat
     1681 ttactttttt caatgcctca tactttgctg ttcgcttatc tgaaacattt aaattcgaaa
     1741 acttcaatcc aatatctaaa ttaaaatcga ttgtttcata ttcaatcaac ccaaaatctt
     1801 gaaaaagaaa agagagggca tttcttttta tcaagcgctt ctctcttcca gttaaatgtt
     1861 ttgtttcttg attaaataaa taaaattgcc ctgaactgaa ttgatcattc aatcctaata
     1921 tattcaataa agtacttttc cctgctccgc tctctccaac tacagtcaca aaatccccag
     1981 catccgcagt gaaacgatcc aaatgaagaa tcgttttgtt tcgaatgatt tttttcgcac
     2041 cttttatcac tatcaacggc tccaattgtt aacctccatt catcagcaat aactgacttt
     2101 tcttttctgt agacaaaata aaaacaactg aaagaataaa atcaatgaag ctaaagaaaa
     2161 aaatattaaa aagatattta ggctctcttt ctataagaaa aaggaagatt acacttgtcg
     2221 ttccccattt gatgaggttc atcatgaaga aatctttata cgtttcaaag agcgaataac
     2281 cacttgaacg aaagatagcg attttccgac aattgtagac aaaatatatg cataacatga
     2341 catagctcaa caagaagaaa gaaaagaaag tgagtaaaaa tatctgtatc aagtaatgaa
     2401 atgatctgct taacactttg acagctatct cttcagtctg agtatacgga aatgataact
     2461 gcaagttatc acgtaactga ctttcttcaa tcagctcaaa taacctttct ttcgacaaag
     2521 agcctatctt gatctttaac ggtggataga tttctccgct gagaatattt ctttcccaat
     2581 acgtactgtt tttcaatgtc agtatttcaa caaccggata atgttcgatc caggggatag
     2641 aaggaataaa tgtataaatg ggttgattgt tttttataaa tagaaatttc ggttcttttt
     2701 cactgatttc ttgataatat tctgttattt gcgccaggtc attttttaaa tctgtatgtc
     2761 tatatcgttc agggatcaat ataacagggt gcgtttcttt ttcggttatt tggatcaacc
     2821 ttccagagac atcaatcaaa ggaaatttct taagataatt cagatttatt tttatccctc
     2881 tgccaaactg ttcattttct tcattcaagt aatctgatac attgactaat atacttccgt
     2941 ttttattcaa agtttcatat aattcctgat cggctgctga tgcaaaacga ttggaataaa
     3001 taaaatccac gtggtttttc ccgatggtta ctggataaaa tacatgatag tcctcaccag
     3061 cgatttcttt tggtgtttca tgggaaacag ctaactgtga agaagaaaaa acgatagttg
     3121 ctaattcgac attggcaacg gtcaaaagaa gagaaaacaa caatccaaat gtaaaaagaa
     3181 tgtattggat atagttgaac gcttctctta acgaagcgat tttttcaact aataaaagag
     3241 aaatcaaaga gataccctat attccgctga acaagaaaac gtacatctgg aacaaataaa
     3301 gaaattgctc cttataatac ataaaaataa aaccaccaat gattcctgca attatgaatg
     3361 taataatgat tttctttttc cccaataaac accacaatac ttgatgtaat gaaaagccat
     3421 tattcaacaa taaactgatt tctttattca tcaataagaa aaaggatgat aacaaaagga
     3481 ataaaagaat caatccaata gttaaataca ctcgataatt atatacgtct gtacttgaaa
     3541 taaccgaata cgaatagcta ttttcaaagt cttcaaaaat attagtggca taatcagtgt
     3601 gaaatgcttg attgaatcgt tccacaaata gtaaaatgaa ccgttcatag gcttcttttt
     3661 ctctcgtcat caagaaaaat tcaccttcaa aatcttttat tttgatcgat tcttcaatgg
     3721 acgttacgcg aaaggaggta ttagaatagg gtaccttctc gtgagaagat ttgacatccc
     3781 cttgataata caatgtcatt tcaagctttg gctgtatctt gaagttccaa gatgtatcta
     3841 cttcattgga ttcatttact gtttttttca tataagaaag ctcagtttct gatgagacat
     3901 tttcaaatac catgattagc tgctcatatt cttcttgccc aataaaatgt tgtggcaatc
     3961 gaatcggagt ataagtatct gtttgatgga aaaattcatt tgtccgatag agtacctgtt
     4021 tatttataat aaccaaggca aaaccactca tgataccggt cactatgatt gtgagtaaaa
     4081 ctgtgaactt ttttttgatt atcaccactc tcccttactt aaatgattct ttgataaaat
     4141 cataccgttt ttcatcaaaa aaacgtatct atcaaatgta taggtcatgt agaataaatc
     4201 tatatactgt agaatcgaaa ggagaagaag atgaacatcg gagaaaaaat acgaacttac
     4261 cgcagaaatt gtaagcttac gcaaaaagaa ttaggcgaaa aaatcggtgt ttccgataaa
     4321 acaatctcta gttgggaaaa ttctcgtaca atgccagacc ttgaaatgct gtcactttta
     4381 catcagactt taggtctgcc gattgatttt ccttctattg aatcagctac cgaatcaata
     4441 gaaccaacat tgccggatga acctcttgtt atcaagaaat cttattcatc aaaacaaccc
     4501 ttctcactca aaaagacact atctttcttt ctgctcgtct tttgtttggt ctatcttttt
     4561 cttatcaatt atcaatataa aaatgaaacg gttgatcgat tcaatccttt tttgaaacca
     4621 caaatcggct ataccacact ccctgtagga tacgtggaaa attccatcga acattgggta
     4681 tgtaatgata tctttgttca tggtacatgg ctgtcttttc acgctggaga ggtagaaaaa
     4741 ggtaagaatc atgctgtaat caaacacaaa ggaaaacacg tcatgaacgt tcgcctgctc
     4801 acttgggaag ccattccctc agattaccaa gaaatcctca tccaagacta tgacgatgcc
     4861 ttaaaaagaa gccctcagga gccagatagg cattaagtag gagatgaacg tagagataga
     4921 aaaaatcaat gcacatgatc ttatcaaaaa aaaacagggg tacaaattaa ttgaaaaagc
     4981 tttgagatga aagttcctaa ataaaaaagt tcagaatgat aggtaaaaaa atgactaact
     5041 attctagtat taggctattt gtattattct tctgttattt ttatatcaaa
//
LOCUS       DAZKOB010000143         5061 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000143 DAZKOB010000000
VERSION     DAZKOB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.146"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..208)
                     /locus_tag="ACG8M6_002724"
     CDS             complement(<1..208)
                     /locus_tag="ACG8M6_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458311.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
     gene            complement(305..757)
                     /locus_tag="ACG8M6_002725"
     CDS             complement(305..757)
                     /locus_tag="ACG8M6_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458312.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(764..1312)
                     /locus_tag="ACG8M6_002726"
     CDS             complement(764..1312)
                     /locus_tag="ACG8M6_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0458313.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(1566..2885)
                     /locus_tag="ACG8M6_002727"
     CDS             complement(1566..2885)
                     /locus_tag="ACG8M6_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0458314.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            3142..3612
                     /locus_tag="ACG8M6_002728"
     CDS             3142..3612
                     /locus_tag="ACG8M6_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0458315.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            3834..4937
                     /locus_tag="ACG8M6_002729"
     CDS             3834..4937
                     /locus_tag="ACG8M6_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0458316.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         1676 a          898 c          924 g         1563 t
ORIGIN      
        1 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
       61 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
      121 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
      181 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
      241 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
      301 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
      361 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
      421 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
      481 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
      541 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
      601 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
      661 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
      721 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
      781 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
      841 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
      901 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
      961 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
     1021 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
     1081 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
     1141 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
     1201 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
     1261 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
     1321 ctccataaaa aataagttac tttttaaata tcttgtttat gacctgaata attcatagct
     1381 ttaattcttt ggcacatttt attgaaatat gtatcactaa tatccctatc aactgatttt
     1441 gactaaaaag ttacttcaaa cattcactgt tcaaaaatat tgaggaataa attttgggtg
     1501 taaggcattt tgagcatact tctccctgga taacttccca gtaaaaaaat cgttagattg
     1561 ttggattaga tccattgact ggctcgcctc aggcgagcaa agaccctgta gaattcattc
     1621 tgatacacat gataactaga taatttgaga tagactcgtc tacccgtttg gactaatttc
     1681 ccagcaactt tcagaaactt caatcgaata gaatgaatgg tcattccctt ttggacttct
     1741 tcaaagccaa tcgttcttaa gaagttgact aagttgtaag ctatcaagct gagcatcatt
     1801 cggacatgat tctccaaaaa gcgaggactg tctgttttgt caaagtaaaa gccagctttc
     1861 gcttctttaa tgaagttctc cattgtgcca cgtttggcat agatagaaaa gatggtatca
     1921 ggagaaacat tttctgatag attcgtcacg ataaattcat gtcgaaagag tagttcgccc
     1981 gcttcacgtg ttgagcgtat acacacgcga cgactttgtg accaggattg tgcttgataa
     2041 gtggtcgaga agtactgaac ttctcgttct tcccactttt gattatcgcc ataaagtact
     2101 gatttctcag ctatttgacc tagtctacga ttattcttca gtcgaataac ataatgactc
     2161 ttttttgatt cacacgaatc atacacacca ggtgtagcga acccgctgtc tccacgaacc
     2221 aagatgtcag tgtttggtag agagtgatta tagtgctcta ataaaggtgt gagaaactcc
     2281 tttacgcctt ttgatgtata ttgatttcct gaacgtagtt cagcttttaa gaagtcacca
     2341 gtcaatccgt caaaagctac caatggatgg taaccatagg tttggtagtg tgtattataa
     2401 ttcgtttgtt cttggtgacc aaatgtatct gaatgggtcg aatctaaatc aatgattaat
     2461 tccgtatcat tacggatgag gcgtgcttta tcgataagtt cttggttcaa agcttgaagt
     2521 tcatggatat tctcctcaga tagtcgatct aaaaatcggg aaagtgaaga ttgagaagcg
     2581 agttctttcc tatctaaaac agctttaaac acaggatctt gtctcaggag attagctgca
     2641 gaatcagctg agtaaccagc aattaattgc ataatgaact gctcaagtat ggataagttg
     2701 tcatgagtaa aatacgctcg ttcgtcttca atgtgaatgt gttgcttagc cagttcagaa
     2761 aaaccaaaga tgttcatcag ctctttcact aggacgagac ccgaatcact cgataattga
     2821 ccacctgtat gagaaatagt gatatttgaa ttgaatttta cttggttttt gtgtaagcta
     2881 gtcatgaaaa gaactccttt cttttggttg gtttagtcac tttaaccata gcagaaaggg
     2941 gttctttttt catcacttaa cgggtgaaga tgaaaaagta attgtaagct gccgtgaggc
     3001 agtttcacat ggttttaatt aaaagtatga attattcagg tagaatctct gaagaataaa
     3061 tcacgtgatc gtgacgataa aatacactta cgtcaggtga taaaaaatac ataagtagaa
     3121 aactaagagg aggaacagaa aatgaaagaa gttcaattga tcagaaaaag cgaactatca
     3181 gaaggtggtt gcaatgcatg cggcgtagta gaagcaacaa gctatacatt aaagcttggt
     3241 gccaacaaag cgataatctc tgagttgaca gtaggcggtc ttgtggacag tctggcgctt
     3301 gctgaaggtt ttattggaga agatatttat gagatgtttt cagaaatccg tcagttaaaa
     3361 aaaggggaaa actgcataga ggttcatcat gagagcccga atgtgagatt caaacgtggt
     3421 gataatgaga tgatcttcaa taatcatgtg tccgatcaca ccgagctata tggaatcgtg
     3481 aatcaaatac taactgagct ttttggattg ggaccttatg cgttcaagga agaaaacggt
     3541 aatccaaaat taaacgaaga atggcaagaa acgattgaaa tacaaagaaa caacccgcac
     3601 ttatttcaat aggcatccac agtatttcgt aaaagggact ttgacacgac ttttgtcaaa
     3661 atcccatcgt tttttatcta tttgtgttct aaacatttct ttcagagtag taacaactgg
     3721 aatgactgaa atgtaaacag attatttgcc tgtatcaata cggaagagta atataaagtg
     3781 tgtaaacgct atcatattat tcattcaatt ttttatagag ggaaggcatt gaaatggaaa
     3841 aaacgtatca agcaggtaca aatgaaggaa tagtggattt tatcaatatg gaagatttag
     3901 aaatagccgc ttcacaagtg attccagctg gtggttatgg ttacatcagc agtggtgctg
     3961 gcgacttatt tacttatcaa gaaaatgaac gggcattcaa tcatcagctg attattcctc
     4021 atgtattaag agatgtagaa ttacccgata caactacaca ttttgacaaa gaaacgctga
     4081 ctgcaccaat catcatggca ccagttgctg ctcatggctt agctcatgtg aaggcggaga
     4141 aagcttctgc aaaaggtgta gcagattttg ggacgatcta tacagcaagt tcttacgctt
     4201 cttgtacatt agaagagata agagaagcag gcggggagaa agcaccgcaa tggtttcaat
     4261 tttatatgag caaagatgat gggatcaatt tggatattct tgaagtggcc aagcgtaatg
     4321 gagcaaaagc aatcgtattg acagcggatg caacagttgg cggaaaccgt gaaacagacc
     4381 gcagaaatgg gtttaccttc ccactgccaa tgccgatcgt tcaggcttat caatcaggag
     4441 taggccaaac gatggatgct gtgtacaaat cttccaaaca gaagcttagt ccaaaagatg
     4501 tagaatttat tgctgctcat tctgatttgc cagtctatgt gaaaggtgtc cagtcagaag
     4561 aagatgtcta tcgttcatta gaatctggtg ctggcggaat ctgggtatct aatcatggtg
     4621 gccgtcaact agatggtggt ccagcagcgt ttgattcgtt gcaatatgtg gcagaagcag
     4681 tagataaacg tgtaccaatc gtgtttgaca gcggtgtacg acgcggacag catgttttca
     4741 aagcaatcgc atcaggtgca gatcttgttg ctattggcag acccgttatt tatggattgt
     4801 ctttaggagg aagtacagga gtccatcaag tgtttgattt ctttaaaaca gaattggaaa
     4861 tggtcatgca attagctgga acacaaacag tagaagatat caaaaaaata aaattgcgtg
     4921 agaatcgttt tatttaagaa acaaatgacg aaaaaaatag gagtgcggca gatgatgccg
     4981 tactcctatt tttatagttc aaggatgtca ctaatagaat aatttgaaac gaaaaaataa
     5041 aattgaaata ttttttcata c
//
LOCUS       DAZKOB010000144         5029 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000144 DAZKOB010000000
VERSION     DAZKOB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.147"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8M6_002730"
     CDS             complement(<1..182)
                     /locus_tag="ACG8M6_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0458317.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8M6_002731"
     CDS             517..951
                     /locus_tag="ACG8M6_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0458318.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8M6_002732"
     CDS             974..1294
                     /locus_tag="ACG8M6_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458319.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8M6_002733"
     CDS             1435..1626
                     /locus_tag="ACG8M6_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458320.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8M6_002734"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8M6_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0458321.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8M6_002735"
     CDS             2112..2375
                     /locus_tag="ACG8M6_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458322.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8M6_002736"
     CDS             2399..2587
                     /locus_tag="ACG8M6_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458323.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8M6_002737"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8M6_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458324.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8M6_002738"
     CDS             3070..3441
                     /locus_tag="ACG8M6_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0458325.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8M6_002739"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8M6_002739"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8M6_002740"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8M6_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458326.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8M6_002741"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8M6_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0458327.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1752 a          784 c          983 g         1510 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatatt
//
LOCUS       DAZKOB010000145         4951 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000145 DAZKOB010000000
VERSION     DAZKOB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.148"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACG8M6_002742"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACG8M6_002742"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0458328.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="ACG8M6_002743"
     CDS             complement(1298..2134)
                     /locus_tag="ACG8M6_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458329.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="ACG8M6_002744"
     CDS             complement(2318..3535)
                     /locus_tag="ACG8M6_002744"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0458330.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3684..3845)
                     /locus_tag="ACG8M6_002745"
     CDS             complement(3684..3845)
                     /locus_tag="ACG8M6_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458331.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3918..4001
                     /locus_tag="ACG8M6_002746"
     tRNA            3918..4001
                     /locus_tag="ACG8M6_002746"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..4951)
                     /locus_tag="ACG8M6_002747"
                     /pseudo
     CDS             complement(4241..4951)
                     /locus_tag="ACG8M6_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1516 a         1024 c          794 g         1617 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa a
//
LOCUS       DAZKOB010000146         4939 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000146 DAZKOB010000000
VERSION     DAZKOB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.149"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..696)
                     /locus_tag="ACG8M6_002748"
     CDS             complement(334..696)
                     /locus_tag="ACG8M6_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458332.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="ACG8M6_002749"
     CDS             complement(770..1150)
                     /locus_tag="ACG8M6_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0458333.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1232..2407
                     /locus_tag="ACG8M6_002750"
     CDS             1232..2407
                     /locus_tag="ACG8M6_002750"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0458334.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2484..3068)
                     /locus_tag="ACG8M6_002751"
     CDS             complement(2484..3068)
                     /locus_tag="ACG8M6_002751"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0458335.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3269..4267
                     /locus_tag="ACG8M6_002752"
     CDS             3269..4267
                     /locus_tag="ACG8M6_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0458336.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4379..4888
                     /locus_tag="ACG8M6_002753"
     CDS             4379..4888
                     /locus_tag="ACG8M6_002753"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0458337.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1569 a          923 c          857 g         1590 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaatc atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca
     2461 taattactcc gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca
     2521 tgtacaggtc ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc
     2581 ccttctagta catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct
     2641 ttatacgctt caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta
     2701 gctggcacat ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa
     2761 tttgccatag ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa
     2821 ctttcgccat aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc
     2881 atgaaatcag gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt
     2941 tcaatgaaat tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca
     3001 ccacggtttg ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg
     3061 aatgccatat tgtttcctcc taatatttgc atacttcata ataataataa cataatacct
     3121 aacctctcgt aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta
     3181 taacttaccg tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct
     3241 gctatagtgt gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc
     3301 aggtcctgta gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat
     3361 cattgacagt ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat
     3421 ttacgatatt cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa
     3481 acaattaact acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg
     3541 tgaagacgaa gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat
     3601 tttagcttta ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc
     3661 atttgaaaac cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa
     3721 agtcgctatt gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat
     3781 tgcaagcgaa gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt
     3841 atcacgcttg aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc
     3901 aggcaataat ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat
     3961 tgatttgatg attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct
     4021 ttcttcttgg ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac
     4081 ttctattcca ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact
     4141 gattgctact gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa
     4201 tccaaaggaa cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc
     4261 tagttgattt tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt
     4321 tttttcatgt tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat
     4381 ggtcgtcaaa actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt
     4441 aactgtagaa gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt
     4501 gactttggcg gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa
     4561 cgccattatc actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc
     4621 gttgcaacat attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt
     4681 gtcaatcgtg aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt
     4741 gaaacctggt attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt
     4801 agatgatatc gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc
     4861 agaaaaaaga agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta
     4921 gacgagttct ttctttttt
//
LOCUS       DAZKOB010000147         4789 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000147 DAZKOB010000000
VERSION     DAZKOB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.152"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..286
                     /locus_tag="ACG8M6_002754"
     CDS             <1..286
                     /locus_tag="ACG8M6_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458338.1"
                     /translation="KSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRFI
                     RMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            345..785
                     /locus_tag="ACG8M6_002755"
     CDS             345..785
                     /locus_tag="ACG8M6_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458339.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            797..1465
                     /locus_tag="ACG8M6_002756"
     CDS             797..1465
                     /locus_tag="ACG8M6_002756"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0458340.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1529..2152
                     /locus_tag="ACG8M6_002757"
     CDS             1529..2152
                     /locus_tag="ACG8M6_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0458341.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2208..2594
                     /locus_tag="ACG8M6_002758"
     CDS             2208..2594
                     /locus_tag="ACG8M6_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0458342.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            2807..4198
                     /locus_tag="ACG8M6_002759"
     CDS             2807..4198
                     /locus_tag="ACG8M6_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0458343.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1590 a          801 c         1009 g         1389 t
ORIGIN      
        1 aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt
       61 gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat
      121 ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga
      181 cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag
      241 aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt
      301 ggattttttt gtcataataa atattagatg atggaggaga taatatgatg caaacatggt
      361 tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga
      421 aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc
      481 gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat
      541 ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat
      601 atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg
      661 atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta
      721 aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg
      781 tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt
      841 tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc
      901 agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc
      961 tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg
     1021 gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa
     1081 aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg
     1141 atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt
     1201 ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc
     1261 cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg
     1321 tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat
     1381 ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac
     1441 aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa
     1501 agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac
     1561 attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg
     1621 tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt
     1681 gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa
     1741 agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa
     1801 agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct
     1861 gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt
     1921 tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag
     1981 aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg
     2041 agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt
     2101 ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct
     2161 ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa
     2221 gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag
     2281 aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag
     2341 aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat
     2401 ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg
     2461 tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt
     2521 cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag
     2581 aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt
     2641 ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat
     2701 aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt
     2761 tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat
     2821 cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa
     2881 gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg
     2941 cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg
     3001 gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt
     3061 gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat
     3121 tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat
     3181 tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac
     3241 agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca
     3301 gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac
     3361 aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa
     3421 aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat
     3481 cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat
     3541 gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt
     3601 tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc
     3661 aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac
     3721 ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg
     3781 agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg
     3841 ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa
     3901 tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg
     3961 tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa
     4021 cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg
     4081 tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa
     4141 agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc
     4201 aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat
     4261 agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg
     4321 tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa
     4381 tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac
     4441 cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa
     4501 aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa
     4561 caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca
     4621 tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt
     4681 atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat
     4741 tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacag
//
LOCUS       DAZKOB010000148         4755 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000148 DAZKOB010000000
VERSION     DAZKOB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.153"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..744
                     /gene="vanR-A"
                     /locus_tag="ACG8M6_002760"
     CDS             49..744
                     /gene="vanR-A"
                     /locus_tag="ACG8M6_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0458344.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            722..1876
                     /locus_tag="ACG8M6_002761"
     CDS             722..1876
                     /locus_tag="ACG8M6_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0458345.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2091..3059
                     /locus_tag="ACG8M6_002762"
     CDS             2091..3059
                     /locus_tag="ACG8M6_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0458346.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3052..4083
                     /gene="vanA"
                     /locus_tag="ACG8M6_002763"
     CDS             3052..4083
                     /gene="vanA"
                     /locus_tag="ACG8M6_002763"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0458347.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4089..4697
                     /locus_tag="ACG8M6_002764"
     CDS             4089..4697
                     /locus_tag="ACG8M6_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0458348.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1470 a          910 c         1115 g         1260 t
ORIGIN      
        1 taatctgata aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa
       61 atacttattg tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac
      121 gagaattata cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag
      181 tctgagattg accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc
      241 tgtcaaaaaa taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca
      301 gaggtagata aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt
      361 cgcccactgg agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt
      421 ggagtaaagg agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac
      481 acccatgagt gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata
      541 ctgcgaatcc tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag
      601 atatggggcg acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat
      661 ttgcgcgaaa aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg
      721 gttggttata aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta
      781 catgtatatc gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat
      841 gatccgaggg aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa
      901 tcacctggac gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat
      961 ttatgtggcg attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc
     1021 aaaatacttt gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca
     1081 aattgagctt tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac
     1141 tctggaaaag cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat
     1201 gtacttggcg cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct
     1261 tgacgaggct ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga
     1321 caaagcgtat cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct
     1381 acaaacgata acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac
     1441 cgatgaattt tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga
     1501 ggatctgacc gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa
     1561 aaacgccgct gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg
     1621 ggatgtggtg tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc
     1681 catatttgaa aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg
     1741 acttggattg gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga
     1801 aagcaatgat aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga
     1861 taaaaggagg tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac
     1921 tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact
     1981 taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt
     2041 ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca
     2101 tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc
     2161 ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat
     2221 ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc
     2281 ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc
     2341 atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc
     2401 cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat
     2461 cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac
     2521 tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc
     2581 ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa
     2641 actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc
     2701 tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag
     2761 catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat
     2821 tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt
     2881 tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa
     2941 tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata
     3001 ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga
     3061 ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct
     3121 gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt
     3181 acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat
     3241 tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac
     3301 catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt
     3361 gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat
     3421 attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct
     3481 gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg
     3541 tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa
     3601 gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa
     3661 atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt
     3721 gccgcgttag ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt
     3781 catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac
     3841 ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc
     3901 ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg
     3961 aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc
     4021 gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg
     4081 tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg
     4141 acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa
     4201 atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg
     4261 ctacccaagg gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact
     4321 gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca
     4381 atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc
     4441 gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg
     4501 ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca
     4561 atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat
     4621 atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg
     4681 atttccccgt taaataaact tttaaccgtt gcacggacaa acggttctgt tgcaaagttt
     4741 taaataaaga ataaa
//
LOCUS       DAZKOB010000149         4747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000149 DAZKOB010000000
VERSION     DAZKOB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.154"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..1649
                     /locus_tag="ACG8M6_002765"
     CDS             159..1649
                     /locus_tag="ACG8M6_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0458349.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            1997..2167
                     /locus_tag="ACG8M6_002766"
     CDS             1997..2167
                     /locus_tag="ACG8M6_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458350.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            2181..2798
                     /locus_tag="ACG8M6_002767"
     CDS             2181..2798
                     /locus_tag="ACG8M6_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0458351.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            2798..3736
                     /locus_tag="ACG8M6_002768"
                     /pseudo
     CDS             2798..3736
                     /locus_tag="ACG8M6_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4429..>4747)
                     /locus_tag="ACG8M6_002769"
     CDS             complement(4429..>4747)
                     /locus_tag="ACG8M6_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFQ0458352.1"
                     /translation="FYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNL
                     NIGNNSSFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVD
                     DWI"
BASE COUNT         1738 a          738 c          860 g         1411 t
ORIGIN      
        1 ataacaaaat aatttattcg attagtggaa aaaaattgac ttataaagga aaaaaatctt
       61 tttcaaaaca tgcaatattg aaacagttga atgaaaaagc aaaccaagtt aattaaacaa
      121 cctattttat aggatttata ggaaaggaga acagctgaat gaatatccct tttgttgtag
      181 aaactgtgct tcatgacggc ttgttaaagt acaaatttaa aaatagtaaa attcgctcaa
      241 tcactaccaa gccaggtaaa agcaaagggg ctatttttgc gtatcgctca aaatcaagca
      301 tgattggcgg tcgtggtgtt gttctgactt ccgaggaagc gattcaagaa aatcaagata
      361 catttacaca ttggacaccc aacgtttatc gttatggaac gtatgcagac gaaaaccgtt
      421 catacacgaa aggacattct gaaaacaatt taagacaaat caataccttc tttattgatt
      481 ttgatattca cacggcaaaa gaaactattt cagcaagcga tattttaaca accgctattg
      541 atttaggttt tatgcctact atgattatca aatctgataa aggttatcaa gcatattttg
      601 ttttagaaac gccagtctat gtgacttcaa aatcagaatt taaatctgtc aaagcagcca
      661 aaataatttc gcaaaatatc cgagaatatt ttggaaagtc tttgccagtt gatctaacgt
      721 gtaatcattt tggtattgct cgcataccaa gaacggacaa tgtagaattt tttgatccta
      781 attaccgtta ttctttcaaa gaatggcaag attggtcttt caaacaaaca gataataagg
      841 gctttactcg ttcaagtcta acggttttaa gcggtacaga aggcaaaaaa caagtagatg
      901 aaccctggtt taatctctta ttgcacgaaa cgaaattttc aggagaaaag ggtttaatag
      961 ggcgtaataa cgtcatgttt accctctctt tagcctactt tagttcaggc tattcaatcg
     1021 aaacgtgcga atataatatg tttgagttta ataatcgatt agatcaaccc ttagaagaaa
     1081 aagaagtaat caaaattgtt agaagtgcct attcagaaaa ctatcaaggg gctaataggg
     1141 aatacattac cattctttgc aaagcttggg tatcaagtga tttaaccagt aaagatttat
     1201 ttgtccgtca agggtggttt aaattcaaga aaaaaagaag cgaacgtcaa cgtgttcatt
     1261 tgtcagaatg gaaagaagat ttaatggctt atattagcga aaaaagcgat gtatacaagc
     1321 cttatttagt gacgaccaaa aaagagatta gagaagtgct aggcattcct gaacggacat
     1381 tagataaatt gctgaaggta ctgaaggcga atcaggaaat tttctttaag attaaaccag
     1441 gaagaaatgg tggcattcaa cttgctagtg ttaaatcatt gttgctatcg atcattaaag
     1501 taaaaaaaga agaaaaagaa agctatataa aggcgctgac aaattctttt gacttagagc
     1561 atacattcat tcaagagact ttaaacaagc tagcagaacg ccctaaaacg gacacacaac
     1621 tcgatttgtt tagctatgat acaggctgaa aataaaaccc gcactatgcc attacattta
     1681 tatctatgat acgtgtttgt tttttctttg ctgtttagcg aatgattagc agaaatatac
     1741 agagtaagat tttaattaat tattaggggg agaaggagag agtagcccga aaacttttag
     1801 ttggcttgga ctgaacgaag tgagggaaag gctactaaaa cgtcgagggg cagtgagagc
     1861 gaagcgaaca cttgattttt taattttcta tcttttatag gtcattagag tatacctatt
     1921 tgtcctaata tgattttagc agtataattg acttggtgaa taggtcattt aagttgggca
     1981 taataggagg agtaaaatga aaaaatttat ttatcgagtt ttagaaaatg acgaagtggt
     2041 ggctattttt aatgagcaac aatatgcgca agattttatc gcttacgaaa agacaatttc
     2101 tgataagcaa tttgaaattg aaaaagtaga tattgctgat tggttattgc aaccgagaga
     2161 attttagagg ttggttgaaa atggctaaaa ttggttatgc acgtgtcagt agcaaagaac
     2221 agaacttaga tcggcaatta caagcgttac agggcgtttc taaggtcttt tcagacaaat
     2281 taagcggtca atcggtcgaa cgcccacaat tacaagctat gcttaactat attcgtgaag
     2341 gggatattgt tattgttact gaattagatc gattaggacg aaataataaa gaattaacag
     2401 aattgatgaa tcaaattcaa attaaggggg caaccctgga agtcttaaat ttaccctcaa
     2461 tgaatggtat tgaagatgaa aatttaaggc gtttgattaa tagccttgtc attgaattgt
     2521 acaagtatca agcagaatca gaacgaaaaa aaattaagga acgtcaggca caaggaatcg
     2581 aaattgctaa gaaaaaaggc aaattcaaag gtcgtcagca taaatttaaa gaaaatgatc
     2641 cacgtttaaa gcatgctttc gatttgtttt tgaatggttt atccgataaa gaagttgaag
     2701 aacaaactgg aatcaatcgc cgaacgttta gaaggtatcg agcaagatat aacgtgacag
     2761 tcgatcaaag aaaaaacaat gaaaagaggg atagttaatg agtacggtta ttttagctga
     2821 aaaaccaagc caggcattag cctacgcaag tgctttaaaa caaagcacca aaaaagacgg
     2881 ttattttgag atcaaagacc cactatttac agatgaaacg tttatcacct ttggttttgg
     2941 gcatttagtg gaattagcag aaccaggtca ttatgacgaa aagtggcaaa attggaaact
     3001 tgaatctttg ccgatttttc ctgatcgata cgattttgaa gttgcaaaag ataagggaaa
     3061 gcagtttaaa attgttgcag aacttctcaa aaaggcaaat acaattattg ttgcaacaga
     3121 tagcgacaga gaaggtgaaa atatcgcctg gtcgattatc cataaagcaa atgccttttc
     3181 aaaagataaa acatttaaaa gactatggat caatagctta gaaaaagatg taatccgaag
     3241 cggttttcaa aatttgcaac ctggaatgaa ttactatccc ttttatcaag aagcgcaaac
     3301 acgccaaatt gccgattggt tgatcggcat gaacgcaagc cctttgtata cgttaaattt
     3361 acaacagaag ggcgtacaag gtacattttc actaggacgt gttcaaacgc ccaccttata
     3421 ccttattttt cagcgccagg aagccataga gaattttaaa aaagaacctt ttttcgaggt
     3481 ggaagctagt ataaaagtaa accaagggtc gtttaagggc gttctaagcc ccacacagcg
     3541 ttttaaaacc caagaggagc ttttagcttt tgtttcttct aaacaagcta aaataggcaa
     3601 tcaagagggg ataattgctg atgttcaaac caaagagaag aaaacgaata gtccgagttt
     3661 gttttcttta agtagtttgc aatcaaaagt caatcagctt tataaagcga cagcgagcca
     3721 aactttaaaa gctatttctt ttttaataac ttaaaaataa acttaatgta acagcaagca
     3781 cagtcaaggt atacaccttt gacaaaaaat agcacattct ctatcgaaaa tttttgctta
     3841 ttttttaaat tattttggga aattttccca atcccttttt ctaactcaaa aaatataatc
     3901 actcaaaatt taaaagggcg cacttataca tcattttaaa aaattgatgt aacgtgctaa
     3961 gttcaaaaca aagggcgcac ttatacacga ttttcaatct tgtatatttc taacgaaaag
     4021 cgtgcgccaa aaaaccccct tcgtcaattt tgacaggggg ctttttgatg taaaaatttc
     4081 tatcgaaatt taaaaattcg cttcactcat gttataaaga cttaaaataa aataactctt
     4141 taaaatcttt tgctagttgt tcttcaatat tttttattcg gtgcatcttc caagtaaagt
     4201 ataacacact agacttattt actacgtttc ataagtcatt aatgcgtgtg ctctgcgagg
     4261 ctagtttttg tgcaagcaca aaaaatggac tgaataaatc agtccataag ttcaaaacca
     4321 aattcaaaat caaaaccaca agcaaccaaa aaatgtggtt gttatacgtt cataaatttt
     4381 atgatcactt acgtgtataa aattaaattc actttcaaaa tctaaaaact aaatccaatc
     4441 atctacccta tgaattatat cttgaaattc attcataaat agtgaagcat ggtaaccatc
     4501 acatacagaa tgatgaagtt gcagagcaac tggtatataa attttattat tctcactata
     4561 aaatttacct atcgtaataa taggcaataa aaagctgcta ttgttaccaa tatttaaatt
     4621 aaatgaacta aaatcaatcc aaggaatcat tgaaatcggt atggtgtttt caggtatcgg
     4681 ttttttagga aacatttctt ctttatcttt atattcaagc aagtcatttt tataattatt
     4741 ataaaaa
//
LOCUS       DAZKOB010000150         4523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000150 DAZKOB010000000
VERSION     DAZKOB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.156"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1316
                     /locus_tag="ACG8M6_002770"
     CDS             156..1316
                     /locus_tag="ACG8M6_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0458353.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLENHFTQYTFDQVV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            1351..2118
                     /locus_tag="ACG8M6_002771"
     CDS             1351..2118
                     /locus_tag="ACG8M6_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0458354.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            2310..2708
                     /locus_tag="ACG8M6_002772"
     CDS             2310..2708
                     /locus_tag="ACG8M6_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFQ0458355.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(3015..3389)
                     /locus_tag="ACG8M6_002773"
     CDS             complement(3015..3389)
                     /locus_tag="ACG8M6_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458356.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(3411..3761)
                     /locus_tag="ACG8M6_002774"
     CDS             complement(3411..3761)
                     /locus_tag="ACG8M6_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458357.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            complement(3778..4173)
                     /locus_tag="ACG8M6_002775"
     CDS             complement(3778..4173)
                     /locus_tag="ACG8M6_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFQ0458358.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(4240..4425)
                     /locus_tag="ACG8M6_002776"
     CDS             complement(4240..4425)
                     /locus_tag="ACG8M6_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFQ0458359.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
BASE COUNT         1547 a          685 c          773 g         1518 t
ORIGIN      
        1 aataaaatgt gttttactga cattgagtta tgttataaat ttacgataca tttcttattg
       61 taacgtatcg aaaacaggtc tatacttatt caatggaatc gtaccagttc caagttttta
      121 agctcgtacc aagcttagaa agaagaggca atcgtatgaa aataacagaa aagaatgtaa
      181 acccgaaatt tgaaactacc gaaagcaatc taaaaaagtt tgatttaact gttactttat
      241 cgaatcaact tgtagatagc attgttgaaa tggatgctat gccacaaaaa atttatcttt
      301 acgctatagg acatcttgac cctaaaaatc cgccttttaa taataccgtc aaagtatcta
      361 aaaaggcttt atttgcgttg atgtcttcta gttcatcttc ttcaaaacat tcaagattaa
      421 aacaagttat tgaaaaattg catcagcaat ctgtcattaa aattcaaaga actaaaccta
      481 acggtaagac tgaatacaaa gtcatctcac cgctagaaat tactgaattc aatgattttt
      541 ctgatgcagt atctattaaa tttagtagtt caattatgcc tttattaacg aacttagaaa
      601 atcattttac gcaatataca tttgatcaag tagttaactt gacaagcaag tatgcgatca
      661 gtttttttca attattcact aaagatttta atctgtatga agcaaataga gataacttaa
      721 catggagtac aaaatcactt gatagattaa aaaatcctgt tttctccatt gcagatttac
      781 gaaagaaatt tagtattgag aaaaaatata aagcttttcg agactttgaa agacgtgtaa
      841 ttaaagaacc gattgcccaa ctgaataaat acactgattt taaaattgac tatgagctta
      901 tcaagaaggg tcatacttat acaaacataa agttcttgct tacacgtacc atgtcagttc
      961 cggtacctta taagcaagaa caaagagacc ctgcttattt aaaaggcgca gaagataaaa
     1021 cagagaaaat gaatcaatcg tacttgaaag ctatgaatag caaatacact aaattactaa
     1081 tcaaaaactt tttgatcagt tctgccgact tatgcgatct taaacttatg agccgacttc
     1141 agaataaagt ttatccgctt tatgatcgtt tatccaaaga tagaggattt gaatcagttg
     1201 tcaatcacct atcttacgtt gctactcata aggaggatta cacaataaaa aatactgtta
     1261 aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat gattaacaac
     1321 tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac aaagtacaat
     1381 gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga actttatcaa
     1441 ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt agatttacct
     1501 gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga actaaaaacc
     1561 atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga caaaaaatat
     1621 aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga agagcatagt
     1681 gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa aaagaagtat
     1741 ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat cgtgttatac
     1801 aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg agattttgag
     1861 aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga gattacgaaa
     1921 acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag ggaatcagtt
     1981 gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag aaaaggcaga
     2041 aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa aacttataga
     2101 gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct tcagacgtat
     2161 gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt taattagctt
     2221 gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa aaagcaaaaa
     2281 ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca gtattcgaat
     2341 ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat acacatggtg
     2401 aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac ttggtgcttt
     2461 tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta aatgcaagaa
     2521 tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa gttgctaaag
     2581 ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat ttaaatgttt
     2641 tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg agagaaaagt
     2701 tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta ggacgtatgg
     2761 atttaagaaa gacaaattat caaagaatat attaaaagct ctcagaagcc ttctgagagc
     2821 ttttaaattg ctatgtattg tactgcatct gctcaaattg attttctttt catgcttacc
     2881 atgtatctaa ttttccatca agtaattttt agatatgtat tgagtaataa atagcaaatt
     2941 tgcttgatat aagctcgaat taggtgcttt gtaatgtgtt aggataaaat tatacaagaa
     3001 aatgtcaaaa gctctcagaa cgcttctgag agctttttat cggtaggctt gtttaacgtg
     3061 atcttgaagg ataccctttt gatgtttctc tcgatccctg catcgctaat tactaagtgt
     3121 tcttcaagtg tgatgtaggt atataaatta agcatcaaaa tggtgtagat aagcaaaaaa
     3181 ctccctaaaa ctaagtgttg attataaaaa tctgacttat aaaaaagaac tgagaatgtt
     3241 agtgttgatt cccaaaaaag aaacaaaatt ttgtatttcc aaagatagtg gatcaagcgc
     3301 tgtgctttac ccatctcaaa taatttataa taacctgcta attgatcagc aagcgtatcg
     3361 ttaacggttt ctgtttgtgt ttgtttcatc tttctcttct cctgtttcaa ttaatctaaa
     3421 atttcagatt gagcaagcaa ctcattgtgg tagtcggttt gaaattcaaa taacaatttt
     3481 gccttcttaa cgaggtttaa agcgttcgtt agtgattcac ttaatgcttc aacatcttta
     3541 actgattttg catgtttcat tttttcagtg tgcgtatagc agaattcagg tattgagtta
     3601 ggggttgcct gttcaataaa catctcaatt cgcaagttat tatcctcaaa aataattaga
     3661 tcattggcaa agtgtttggt actaacgcta atgccagttg tatcttttaa tagattattg
     3721 attaacgttc gagagaaata gtttaagtgt gtagctttca taggttcggt ctccttttta
     3781 cttttcaagc aactcttctt ctagtgcttt ttgaagtagt tgagagaagt ttatgttttt
     3841 ctccttccct aaaagaacta accattccgg aacactaaca ttttttctaa ttgtttttgt
     3901 atttaagctt cttcggaaaa gtttaatgtt tagttggatg attactacca cttggttggc
     3961 tttcaaagtt aattcttcca ctgctgatgg tttaggaaat tctgcatctg gtacactata
     4021 tagcgctaat cctaaagcat ccttagcgtt taaatatgct tcttcaagac tatttccaaa
     4081 ggtagatatt tccggtaagt cagggaaaac gatgttataa gcttctcctt ctttctcgaa
     4141 gattgccgga taaaaaatat tttttgtctt catttcctcg ctccttttct agtgtaagag
     4201 aggatagtat gcctaatacc aaataggaag gcaaggctgt taaagccctg cttgcttcca
     4261 gatattagct tctgttccta gtggtacatc accattgtgg atcggaacaa cgaccgttct
     4321 tttagtcttg gaattataaa attttgcatg actgccagtt tgtccagtct ttttgaagcc
     4381 gttagatttg agaattttta caatttctct tgctgtctta ggcatagcta ccctcccttt
     4441 acaaaatcta ttatacacat tgttgtgtat ggaatcaata gaaaaagagg atgttttaac
     4501 ccgtggtgct agttaatttt ttc
//
LOCUS       DAZKOB010000151         4484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000151 DAZKOB010000000
VERSION     DAZKOB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.157"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8M6_002777"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG8M6_002777"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0458360.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACG8M6_002778"
     CDS             complement(1034..1954)
                     /locus_tag="ACG8M6_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0458361.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACG8M6_002779"
     CDS             2067..2819
                     /locus_tag="ACG8M6_002779"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0458362.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3683..4216
                     /locus_tag="ACG8M6_002780"
     CDS             3683..4216
                     /locus_tag="ACG8M6_002780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0458363.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1323 a          878 c          795 g         1488 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta
     3061 ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag
     3121 ttcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     3181 ttattaagag gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa
     3241 tagttcctca tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac
     3301 cgtttattga gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca
     3361 aaatagttcc tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca
     3421 ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg
     3481 aaaatggttc ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca
     3541 tctaactcca aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat
     3601 aaaaaattct atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat
     3661 ttcccaagga ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca
     3721 tcagccttat agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga
     3781 tgaattctat acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca
     3841 cggaatccat caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat
     3901 ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa
     3961 ggtcttagcg ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg
     4021 tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt
     4081 gtgcgaaaat ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc
     4141 atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact
     4201 tgtgactata aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt
     4261 tcactccgag tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt
     4321 gactggtgac cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct
     4381 gttctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg
     4441 ttggacttat taccgcagat gcaagctttt ggacaccgtt tacc
//
LOCUS       DAZKOB010000152         4425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000152 DAZKOB010000000
VERSION     DAZKOB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.158"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG8M6_002781"
     CDS             690..1658
                     /locus_tag="ACG8M6_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0458364.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ACG8M6_002782"
     CDS             1669..2484
                     /locus_tag="ACG8M6_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0458365.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG8M6_002783"
     CDS             2552..3262
                     /locus_tag="ACG8M6_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0458366.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG8M6_002784"
     CDS             3298..>4425
                     /locus_tag="ACG8M6_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0458367.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZKOB010000153         4413 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000153 DAZKOB010000000
VERSION     DAZKOB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.159"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..1007
                     /locus_tag="ACG8M6_002785"
     CDS             279..1007
                     /locus_tag="ACG8M6_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0458368.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1152..3179
                     /locus_tag="ACG8M6_002786"
     CDS             1152..3179
                     /locus_tag="ACG8M6_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0458369.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            3287..4384
                     /locus_tag="ACG8M6_002787"
     CDS             3287..4384
                     /locus_tag="ACG8M6_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0458370.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1547 a          718 c          913 g         1235 t
ORIGIN      
        1 ttttacactc tcgtcaagtc acattttgat agataggagg gggacttacg aattatttta
       61 aaggctatga cagctgtatt tctagattgt gtttgcatca agttgcaata aaaactagaa
      121 atcaaatcgg gctgataatc atttataact aatcgataca taggaccaaa tataggtcta
      181 tgttctttta tttcataagt atcgtaaaac agaattttgc tatggattaa tccaatcgac
      241 tgatatgttt agtaaataaa gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa
      301 cagcagcaat gatgttattc agcatggtag caagtacgac ttttgtttct gcaactgaag
      361 tatacccaaa ggaatataac acagaaggaa cgattacatt tgaagcagga gatgagggtg
      421 taactcctcc tgttgatcca gagaatcctg atcccaataa acctgtcgat ccatcagatc
      481 ctccaagccc tggaacagga ggggcattat cgattgatta tggatcaaaa ttcaaatttg
      541 ggactcaaaa aatttctact gcggataaaa cgtattatgc agctgctgat gtgatgaacg
      601 atgggtctag aaaaccgaca tatgtccaag tgacagatag gagaagtacc ttatctgggt
      661 ggaaattaag tgtctctcaa ccagaacagt ttaaaactgc cagtggggat gaactagttg
      721 gagcacagct gaaatttacg aaagggcagg ctgtttcttt ggttgatcca acatatacgc
      781 cgcaaacggt gaattctgaa ttaacgttga caccgggagg aaacaatacg ttagcaatta
      841 atgctaaatc aggaacaggt gtggggactt gggtctatcg atttggtgca aatgaaaatg
      901 aaaatcaaga tgcggtacag ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat
      961 attccactaa attagtttgg acattagaag atacacctaa caactagttt actgaaaaga
     1021 actgttaaaa ataaatacgt agtcatattg tcttgcgagg taatcgagca aaaattgtca
     1081 aaggctaggc taaatttgtt tggatacgaa tagttcatct aggaatttaa cgatcaggag
     1141 gaaaagatat attgaaaaag aggaggatcg tgctcatttt aagcagtctc ttagtgacta
     1201 atatctttcc gatcataggg tactctttgg aattgaatca gttggagcat ggaaatagaa
     1261 gagacgaaga aagcaaactc acttcgacat tcgacagttt aatagaatcc aaccagacac
     1321 aagaaagcag tgaggattta agtatttcga gtagtggaga gaccactgac aacgtagaag
     1381 ctgagggttc ggacaaacaa ggactagaag atgcaccaat ttctgcggtt gagctgcctg
     1441 acgatcccaa tattatttct atcgataaaa tttttcaaac acctatcggt actagcacgt
     1501 ccatattgga aaatgggaag ttactgcagt tgaacccagc agaaaggtct caaagaggag
     1561 ctatttggtc aaataagaag attagtttac tctcggattt tacatttaaa tcttatttat
     1621 acttaggaaa tcaacgagat agagcgggag atggcatgac ctttacattg actggcgatc
     1681 ctagaatggc tagtaatccc agtcaagtta ttggatcgcc aggtatggga attggctcat
     1741 attctactcg ttctggccaa ccttacattg cgaatgcgct ctcgattgag tttgatacgt
     1801 ataaaaatag tggttcttct gatcgaatgg atcgagagat aactgctgat aatagagggc
     1861 atggacatgt cgcttttgtc acgcctaaac caaataataa taattattct ggagagcata
     1921 ctggcgtgac ggtagctcct acatttcttt ctaatggcac atggcgcttg ttaataatta
     1981 aatgggacgc ggagcaaaga agtcttactt atgatttgga aggtgtcggt cgctcttctc
     2041 ggattatacc tgatctcatt aggcagtttg gttctacaga agtaaattgg gggtttactt
     2101 cttcaacagg tacttttttt caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg
     2161 tcacatcaac agctgatgta aaagtcaacg atagtgatta tggggccatc gctgaagctg
     2221 gaggaaacga taagataact atcagaaata cattgaaggt cgagggggat tttcttgaat
     2281 ataagaaaat gcagactagt atcgacctcc cacaagaatt aactattccc gagcatatct
     2341 tgatcaatgg aaatcaaatt gatgccaaag atatcttgat aaccggaaat cgagtcacgc
     2401 taaatctgac agattatttt attgctggag atacgttaat aatcgaattg gagacaactt
     2461 tgcaggaagt aattcctgaa aaagtactca ctatgcattt tgaatacctt gaaaacggga
     2521 atttgattga gcaatcgaat gaaataaacc taaagatcgc aaagctgaag gagatagata
     2581 tccatgttta ttataaagat aaagacagtc agattgatat tgctgaaatg aaaacaatca
     2641 ctgggaaaat aggagagaag tataaagaaa ctcctattga tattgaaggg tatgtttttg
     2701 actcggattc agcgaataca gaaggcatct tctccgaaaa tacagaggat atctattttt
     2761 attacagaaa aggcaaactt tatctctcaa ctgctcctca atatgtcaat tttggaaaac
     2821 ataaaatatc cagtagacca ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta
     2881 tagatgaacg ggcttttgga agttggaaat tacaattgaa acaagagaaa gttcttacaa
     2941 atggagattt tgagcttcca gaagctcttt cctttgttac ctctgaaaat acaacggtta
     3001 tagggacatc cgctgttacg attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct
     3061 ctcttttaga ttctacagct catcgtggaa tcagaataaa tattcctgta gaaaaccaaa
     3121 gaatcggaac ctttgagggg aaactatctt ggatacttgc agatgtccca ggaaattaac
     3181 aggaagagaa gaatataaca aaattattga gtttcaagaa gggcacaggt accataggct
     3241 cttgtgtccc acagagacaa gattttgtta tagtcttctc ttacatatga ttaaaggagc
     3301 gattttcttg agaccaaaac aacaattatt tataacactc ctagttattt taggggcact
     3361 ttttaacaat atatcagtgt cagctagtgc atttaatttt tcagtgacgc cgataacttc
     3421 agagaatcaa atcgacaaga gaaaaactta cttcgatctt caacttgatc cggatcagga
     3481 agtagaagta aaagcggaat tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc
     3541 tgtgaatagc gcaaccacca attcaaatgt gatggttgaa tacggaaaaa acgagattga
     3601 aaaagacatg agcctgatct ttgatctggt agattatgtg agctatccac agatagtgac
     3661 gctaaaacct aaaagtgtcc aaaccgttgc ttttcatgtc aggatgccga atgagcgatt
     3721 tgatggtgta ttggctggtg ggatcacatt ccaagaaatc cagacggaaa aggaccaaac
     3781 agaaactaag gatcaaagct tatcgattga gaatgaatac acctatatta ttgctttgtt
     3841 gatgcaacag acactgaacg aagttgctcc gaaccttact cttcatgaag ttaaaccaga
     3901 tcaaatcaat gcaagaaatg tcatcttagc aaacgttcaa aatgatcaaa aaacctatat
     3961 caatcaagta gttattgaaa caaaaattac aaaaaaagga cattctgaag ttttatatca
     4021 agaggaaaaa gaaggattgc agattgcgcc gaatacaaat ttttcttttc caactgcttt
     4081 aaatggtcaa ccgctaaccc caggtgaata tcaccttaca atgactgttt taggaaatga
     4141 aaatgaaaat ggaaaatttt cgagaaaaaa aggaaatact acgatcaact ataccaatca
     4201 atgggtattt gaaaaagatt ttacaataga cggaaaagta gccaaggaat tgaacacgac
     4261 ggatgttacg attaaaaaag acaataattg gatttatatc ttagtagctt cacttttctt
     4321 attgtttatt ttattatgca tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa
     4381 gtaaggttaa gataacaaat agtaaatttt tat
//
LOCUS       DAZKOB010000154         4380 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000154 DAZKOB010000000
VERSION     DAZKOB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.160"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG8M6_002788"
     CDS             73..234
                     /locus_tag="ACG8M6_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0458371.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG8M6_002789"
     CDS             253..1530
                     /locus_tag="ACG8M6_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0458372.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2199
                     /locus_tag="ACG8M6_002790"
     CDS             1540..2199
                     /locus_tag="ACG8M6_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0458373.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYYSNHRKNNISLVFQNYNLIDYLSPIENI
                     RLVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGN
                     LDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEV
                     E"
     gene            2199..3575
                     /locus_tag="ACG8M6_002791"
     CDS             2199..3575
                     /locus_tag="ACG8M6_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0458374.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3705..4118
                     /locus_tag="ACG8M6_002792"
     CDS             3705..4118
                     /locus_tag="ACG8M6_002792"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0458375.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4143..4380)
                     /locus_tag="ACG8M6_002793"
                     /pseudo
     CDS             complement(4143..4380)
                     /locus_tag="ACG8M6_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1700 a          522 c          761 g         1397 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata ttatagtaat catagaaaaa ataatatatc tttagtattt
     1801 caaaactata atttaataga ttatttatca ccaattgaaa atattagatt agtaaataaa
     1861 tcagcagatg aaagtatctt gtttgaacta ggtttagata aaaaacaaat aaaaagaaat
     1921 gttatgaaat tatctggtgg acagcaacaa agagtagcta ttgctagagc attggtatca
     1981 gatgctccaa taatactagc tgatgagcct actggtaatc tagacagtgt tactgctgga
     2041 gaaataatta atatattaaa gacattagct aaagatagaa ataaatgtgt aatagttgta
     2101 acacatagta aggaagtagc agattctgcg gatatcattt tagaactaag tggtaaaaag
     2161 ttgaaaaaag taaataaaat gaatttggag gttgaataat gataaaaaat gcatttgctt
     2221 atgtaactag aaaaagttta aaatcattaa ttatcatatt agttatttta tctatgtcaa
     2281 ctttaagtct cattagttta tctattaaag atgctacgga cagagcttca aaagaaacat
     2341 ttgctaatat aacaaatagt ttttctatgg agataaatag acaagtaaat ccaggaacac
     2401 ctagaggtgg gggaaatgta aaaggtgaag atattaaaaa gatatctcaa acagatagta
     2461 tagactccta tgtaaaaaga ataaacagtg ttgcagattt agttgattat gatattattg
     2521 aaactcaaga gactcttgcg aatcaatcac ctgaaagagc aaagaatttt aaaagaacag
     2581 ttatgttaac tggggttaac gactcaacaa aagaaacaaa atttgtatca gaagcatata
     2641 aattagtaga aggaaagcat ttagaaaatg aagataaaaa taaaatctta atgcataaag
     2701 atttagctga aaaaaataac cttaaagttg gagataagat aaaaataaaa tctaatttat
     2761 ttgatgctga taatgagaaa ggtgcagatg aaacagtaga agtagaaatt aaaggtctat
     2821 tcgatgggca taatagcggt gtagtaagtg cagcacaaga actatacgaa aatacattaa
     2881 tcactgatat tcatacagct gctaaagttt atggtaatac cgaagataca gctgtatacc
     2941 aagatgcaac attctttgta aaaggtgata agaatcttga tagtgtaata aaagatcttg
     3001 ggaaattaga tataaattgg agagaatata atttgattaa aagttcatca aattaccctg
     3061 cattacaaca atctatatca ggtatttact caatttcaaa taaattattt tttggctcat
     3121 taatatttgc aggagttgta gtttcattat tattattctt atggatgaat gctagaaaga
     3181 aagaaatagc agtattacta tcattaggta tatcaaaatt agaaattttt ggtcaattcc
     3241 taattgagat ggtatttata tcaatccccg cattcgttgg ttcctatttc ttagctcaat
     3301 atacagctga caagcttggg aataatatat tgaataaggt tactggtgat atagctaaac
     3361 aaatagctaa acaatctgca tctagccaat taggtggtgg ggcagaagct gaaggattta
     3421 acaagacatt atcaagttta gatatcaatg tattacctaa attcatgatt tatgtagtta
     3481 tatttatgag tttagtactt cttgtatcat tgattatttc ttcattcaat atattaagaa
     3541 ggaatccaaa agaattatta attgacaata attaaaattt tcggtgcttt ttagcaccaa
     3601 aaatgagagt gtaatataaa ccgtgtaagt aaccatcaat cctaggcggg tggttgcttc
     3661 acggttttta tttttctaaa ataagatcaa gaggtgatta cacgatggct agaaagaaaa
     3721 gaaatcctga tgctgagaag ttagctgaat ccattttgaa tacttatcaa cccgaatctg
     3781 tagaggatat gcaagatgtt ttaaaagatg tgtttggtcc cttatttgaa aaaatgcttc
     3841 aaggagaatt gaataaccat ttagggtatg acgcccattc taaagagcca aagaaacatg
     3901 acaatcgacg aaacggttat ggcaataaga cgcttaaaac gagctttggt aaagtaaaca
     3961 ttgatgttcc cagagaccgg gaggcttctt ttgaacctga gttgattcct aatagaaaac
     4021 gagatgtctc cgacatcgca gactgtagac aaaagctgtt ccaaactcgt aactcgagct
     4081 tggaacagct ttttatctac ttcgtttcga atttttgaac aaaaaaataa aggctccttg
     4141 cctttccata gccaggtggc taatttcttt aaattcattg cagcaaacac tagcatcgta
     4201 tgcgtcgcga ctttttccaa gcctcgatac ttggtccaac gcatcccatg cttttctttt
     4261 gcatccgcaa atattcgttc aatcgtttgt ttccgttttt tgtaaattga tttattcaga
     4321 tctgttaggc gaagatgttc cacttcatcc atcagatctc cccaaacatg tctcgtgatg
//
LOCUS       DAZKOB010000155         4339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000155 DAZKOB010000000
VERSION     DAZKOB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.161"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..1894)
                     /locus_tag="ACG8M6_002794"
     CDS             complement(128..1894)
                     /locus_tag="ACG8M6_002794"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0458376.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1854..3608)
                     /locus_tag="ACG8M6_002795"
     CDS             complement(1854..3608)
                     /locus_tag="ACG8M6_002795"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0458377.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3718..4287)
                     /locus_tag="ACG8M6_002796"
     CDS             complement(3718..4287)
                     /locus_tag="ACG8M6_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0458378.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1466 a          819 c          603 g         1451 t
ORIGIN      
        1 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
       61 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
      121 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
      181 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
      241 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
      301 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
      361 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
      421 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
      481 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
      541 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
      601 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
      661 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
      721 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
      781 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
      841 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
      901 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
      961 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
     1021 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
     1081 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
     1141 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
     1201 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
     1261 attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat
     1321 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
     1381 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
     1441 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
     1501 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
     1561 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
     1621 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
     1681 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
     1741 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
     1801 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
     1861 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
     1921 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
     1981 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
     2041 actgtttttt ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga
     2101 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
     2161 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
     2221 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
     2281 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
     2341 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
     2401 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
     2461 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
     2521 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
     2581 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
     2641 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
     2701 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
     2761 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
     2821 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
     2881 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
     2941 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
     3001 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
     3061 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
     3121 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
     3181 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
     3241 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
     3301 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
     3361 aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca
     3421 acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat tagagactga
     3481 agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat
     3541 aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat
     3601 actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat
     3661 gctttccgta atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa ctacgttcta
     3721 ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc
     3781 tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat
     3841 tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca
     3901 tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg
     3961 aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact
     4021 aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa
     4081 aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa
     4141 tatgattcca gataataatc caaaaatccc gggggatatg ctgtgaccaa acgccccacc
     4201 aatactacta accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc
     4261 aataaaaata taatcttttg ttttcatata tattctcctc aattctattt cgttatgtca
     4321 ttaaaaatct gttctatca
//
LOCUS       DAZKOB010000156         4297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000156 DAZKOB010000000
VERSION     DAZKOB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.162"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..923)
                     /locus_tag="ACG8M6_002797"
     CDS             complement(27..923)
                     /locus_tag="ACG8M6_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0458379.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(916..1596)
                     /locus_tag="ACG8M6_002798"
     CDS             complement(916..1596)
                     /locus_tag="ACG8M6_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0458380.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1597..2379)
                     /locus_tag="ACG8M6_002799"
     CDS             complement(1597..2379)
                     /locus_tag="ACG8M6_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0458381.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2372..3289)
                     /locus_tag="ACG8M6_002800"
     CDS             complement(2372..3289)
                     /locus_tag="ACG8M6_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0458382.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3453..3707)
                     /locus_tag="ACG8M6_002801"
     CDS             complement(3453..3707)
                     /locus_tag="ACG8M6_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458383.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1257 a          943 c          753 g         1344 t
ORIGIN      
        1 tactagttct cactaatctt gtataattaa accctccgca acttgacgga gatggagaag
       61 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
      121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
      181 cgctggaaga ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt
      241 aatcgaatga atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
      301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
      361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
      421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
      481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
      541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
      601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
      661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
      721 tcattttcat ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga
      781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
      841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
      901 agaagcaaca gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
      961 agcatgtgcc tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc
     1021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
     1081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
     1141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
     1201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
     1261 taacaaaagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
     1321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
     1381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
     1441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
     1501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc
     1561 gtcttcgata atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa
     1621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
     1681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
     1741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc
     1801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
     1861 tgtcaaagcc actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac
     1921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
     1981 tgtgtaagca agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag
     2041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
     2101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
     2161 cagaagaatc catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac
     2221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
     2281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
     2341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
     2401 gtaagaaata atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg
     2461 cttctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
     2521 cacggctaga ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
     2581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
     2641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg
     2701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
     2761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
     2821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
     2881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
     2941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
     3001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
     3061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
     3121 aacccataat agttctcata aaggtagact tacccgctcc attttcccca atcaatccat
     3181 agatcattcc acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt
     3241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
     3301 tttcttattg atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
     3361 aagaaagtgt aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt
     3421 cctattatca ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc
     3481 aaagtcgttt ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg
     3541 agtaatttta ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt
     3601 agactgtctc gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct
     3661 aatccaagtg gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt
     3721 atatgcgttg gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat
     3781 ccttaaaaac ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca
     3841 aataagaggt tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc
     3901 ctgccaactc ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa
     3961 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa
     4021 tattgtttga tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga
     4081 ccaagaatcc tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg
     4141 aaagtgccag aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga
     4201 ttctcgtaac tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct
     4261 ttacgggctt tcttaagacc tggttttttc ttcgtat
//
LOCUS       DAZKOB010000157         4295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000157 DAZKOB010000000
VERSION     DAZKOB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.163"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG8M6_002802"
     CDS             <1..792
                     /locus_tag="ACG8M6_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0458384.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACG8M6_002803"
     CDS             889..3138
                     /locus_tag="ACG8M6_002803"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0458385.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4295
                     /locus_tag="ACG8M6_002804"
     CDS             3141..>4295
                     /locus_tag="ACG8M6_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0458386.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1394 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctaga
//
LOCUS       DAZKOB010000158         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000158 DAZKOB010000000
VERSION     DAZKOB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.164"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG8M6_002805"
     CDS             complement(37..1257)
                     /locus_tag="ACG8M6_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0458387.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG8M6_002806"
     CDS             complement(1277..1888)
                     /locus_tag="ACG8M6_002806"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0458388.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG8M6_002807"
     CDS             complement(1931..2863)
                     /locus_tag="ACG8M6_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0458389.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG8M6_002808"
     CDS             complement(3284..4144)
                     /locus_tag="ACG8M6_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0458390.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZKOB010000159         4252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000159 DAZKOB010000000
VERSION     DAZKOB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.165"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..522)
                     /locus_tag="ACG8M6_002809"
     CDS             complement(<1..522)
                     /locus_tag="ACG8M6_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0458391.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEK"
     gene            complement(567..923)
                     /locus_tag="ACG8M6_002810"
     CDS             complement(567..923)
                     /locus_tag="ACG8M6_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458392.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1221..2195)
                     /gene="sgrA"
                     /locus_tag="ACG8M6_002811"
     CDS             complement(1221..2195)
                     /gene="sgrA"
                     /locus_tag="ACG8M6_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0458393.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2391..3230)
                     /locus_tag="ACG8M6_002812"
     CDS             complement(2391..3230)
                     /locus_tag="ACG8M6_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0458394.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3415..>4252
                     /locus_tag="ACG8M6_002813"
     CDS             3415..>4252
                     /locus_tag="ACG8M6_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0458395.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMK"
BASE COUNT         1398 a          855 c          630 g         1369 t
ORIGIN      
        1 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
       61 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      121 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      181 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      241 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      301 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      361 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
      421 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
      481 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
      541 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
      601 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
      661 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
      721 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
      781 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
      841 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
      901 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
      961 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1021 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1081 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1141 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1201 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1261 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1321 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1381 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     1441 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     1501 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     1561 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     1621 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     1681 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     1741 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     1801 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     1861 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     1921 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     1981 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2041 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2101 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2161 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2221 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2281 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2341 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     2401 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     2461 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     2521 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     2581 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     2641 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     2701 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     2761 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     2821 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     2881 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     2941 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3001 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3061 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3121 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3181 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3241 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3301 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3361 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     3421 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     3481 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     3541 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     3601 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     3661 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     3721 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     3781 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     3841 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     3901 aaaaaagaag caaatgaagg agaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     3961 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4021 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4081 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4141 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4201 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aa
//
LOCUS       DAZKOB010000160         4215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000160 DAZKOB010000000
VERSION     DAZKOB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.166"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..503
                     /locus_tag="ACG8M6_002814"
     CDS             75..503
                     /locus_tag="ACG8M6_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0458396.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ACG8M6_002815"
     CDS             490..732
                     /locus_tag="ACG8M6_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458397.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ACG8M6_002816"
     CDS             complement(1032..1247)
                     /locus_tag="ACG8M6_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458398.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ACG8M6_002817"
     CDS             1354..1668
                     /locus_tag="ACG8M6_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458399.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ACG8M6_002818"
     CDS             1772..2950
                     /locus_tag="ACG8M6_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0458400.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(3131..3316)
                     /locus_tag="ACG8M6_002819"
     CDS             complement(3131..3316)
                     /locus_tag="ACG8M6_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458401.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            3353..3646
                     /locus_tag="ACG8M6_002820"
     CDS             3353..3646
                     /locus_tag="ACG8M6_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0458402.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3734..3880)
                     /locus_tag="ACG8M6_002821"
                     /pseudo
     CDS             complement(3734..3880)
                     /locus_tag="ACG8M6_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1445 a          627 c          820 g         1323 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agata
//
LOCUS       DAZKOB010000161         4207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000161 DAZKOB010000000
VERSION     DAZKOB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.167"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..444
                     /locus_tag="ACG8M6_002822"
     CDS             7..444
                     /locus_tag="ACG8M6_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458403.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            539..1423
                     /locus_tag="ACG8M6_002823"
     CDS             539..1423
                     /locus_tag="ACG8M6_002823"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0458404.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1645..>4207
                     /locus_tag="ACG8M6_002824"
     CDS             1645..>4207
                     /locus_tag="ACG8M6_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0458405.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1369 a          709 c          953 g         1176 t
ORIGIN      
        1 agataaatgg agtggttttg caaatttctt ttatttctag aagttattca cgtatacgga
       61 tttgtttttc tcacaaataa aaaggcagga agtacaatgg atcatttgta tataaaatat
      121 attcatcaat tttccgtttt ggtattaggc acactaatta tagcggggat tgttgcattt
      181 attattcaag gtcgtgacat attaacagta catcaagagt ttttcagtta tgcatttggt
      241 attcctctga tcgctataag gtctgataac aaaaagtgga agaaacgcta ttacattctc
      301 ttgctatttt tgtgttttta tctgccacct aagattttta ctttattagg tatacaatgc
      361 ctatcatttt cttttatttc ctatatcatt gttttaattt tcctcagttt catagtatgg
      421 gtattttatc ttgtcaaaaa ataaatgaat gtttcatgaa cagtacttca tcacagccta
      481 atttttttct ttatgctatc gttatattaa agaaatcaat cagaagggaa agaaagtgat
      541 gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt gaacaagcaa aggaatttga
      601 gcaattagcg atagcaaaat tggaatcggt gtttgatgaa gtggtcgtct tgcatacaaa
      661 aaaagcaggg gatgcaaaaa actttactcg cgaagcggct acggaagggt atcatagtgt
      721 gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc agtggaatcg ctgaacaaga
      781 acatcggcca aatttcggat ttttcccatt gggtacggtg aatgacttgg ctcgagcatt
      841 agggataccg ctagaacctg aggaagcgat caatcatttt tcgattgaat cagtcaagcc
      901 attggatatt ggaaaaataa atgacgacta ttttatgaat gtcgtcgcaa ttggctctat
      961 ccctgaagca atcaatgatg tagatgcgga gaagaagacg aaatttggaa aactagctta
     1021 tttcatgtca ggaatcaaac agttagcttc cacacaatcc tactcgtttc acgtagaagt
     1081 tgatggaaag aaggaagaaa ttgagagcag tatcttgctg ataggactaa ctaactcagt
     1141 aggcggtttt gaaacattac ttccaaatgc aaaagtggat gatggtaagc tccatttggt
     1201 ttatttaaaa gattcttctt tgatcgatac gcttaaaaca ttgccagacc tattaaaagg
     1261 agtagatgaa tctacgaaaa atctagccta tcttacttgt gagcaattag ttgtgtcatt
     1321 agctgatgag gaagaattga cgacgaatgt tgatggagat gaaggggata aattacctgt
     1381 gacaatcaaa gtgttaccct cacatttaca ggtttattgt taaaatggag gttgtgaccg
     1441 aagagttgtc aggaggacat cggttgttcg gaataaggga ctgtgacaag acttttgtca
     1501 cagccttttt ttgtacaaaa aagaaatttt cacctaacag aaaaatttgc cataaaaata
     1561 catattttaa atatgaaagt acaattacaa ataaattaaa acgttaaaat aaaattgtca
     1621 aaacgtcaga gaggatggtg gagaatgaat caatatgtaa aaaagctgat tgcgtcgatt
     1681 ttgtttgtct tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaagc
     1741 gaatcaacgc aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcaatt
     1801 acttctgaaa ctacagaatc ccaaagtgag gcgcctactt ctaatacaag gcaagaagaa
     1861 aataagacaa gtgagacctc agaagtagct gaacaaacgt ctgatagcag tgaagaggta
     1921 caggttagag ttgtttcagc ggatgggtac agcgaagctg ccacaacaga agaactgacg
     1981 cagcttctcg cagacgaatc agtagctaag attcgtttaa ctcagccttt gactttagac
     2041 agggaattgg agatcaagcg agatatcgtt atcgattttg gcggacttgc tcataatttc
     2101 ggcacacatc acatctatat caatgagaca cctcagtcga tcgagtttca gaattttaaa
     2161 ggaacggcag ctcatccagg tgggttgata ccaaatatcg atggaaatgc cattatcatt
     2221 gcttatatgt ccgatttttg gggaaacaga tatcatttta cgggagaaat aaaatttacc
     2281 gggacacttg acttatatga tggttctaaa ttaggcctga tttacgcccc tcgtgcaact
     2341 gtcacattag atggtgtttc cggtgtattg gatgtccaac cagtgaaaga agggatgaat
     2401 gccgctccgg gtaaagctta tttttgtaga agctatcagc taaatgtaat caatggtagt
     2461 cagctttatg gaccatatct aggaaaattc tatggttttc tagattcagg tgatgagacg
     2521 ggcagtggaa aaacagcacc aggactccat atcttgagtg gttcaaaggt ctctcttgac
     2581 tatgatcggg cagatggcgt cagcgatgaa ggagaggctg tcgatacgct acccgggaat
     2641 gtcacattta aagtcagtgg ctcaggaagt gaattttcgg taaatacaaa gatcaatatt
     2701 accaacgaca acaatcgcgg aattatccaa atgagaggct caggatcaag agtcgctgtg
     2761 tcgaatgatg ggaaaatcac aatcaataca gcgactactg gcggatttcg cctgcaagga
     2821 gattctagtc aaatccatgt atcaagtggt ggacaaatct cggtcaaaat ggctggagat
     2881 ggcgaccaac ctggaaacaa tgggatgcgt tttgtaggga aaggactttc tttgatcgta
     2941 gaaggagccg gatcagtcat cgatattgat aagcaaagtg gtcgttctag cgcagttcgt
     3001 tttgagaacg gtacgcaaaa actaacagta actaatggag gctcattgaa ggtaagaaat
     3061 gcaggggatg gcaaatctta tgtgaacaga ctcaatcaag gaaaccaagc gatccaattt
     3121 tatgacgctt ctggttttct agaaagtcca gggtcagctg attttacggt tactggagaa
     3181 aaatcaaata tcgatatacg cgcagattcc ggagctaccg tagatacaac tggagatatt
     3241 gatttgaact ttatggctgg cgaaaaaact tatttagtca tggtcgggaa aactggggaa
     3301 gataagactg gcatcatcag tggggatgtg ttgaatgtag acttagaatc tcctacctat
     3361 ttcgatttcc aaaacaaacg gatcggaagc gagactaatg gcataggtgg ctgggtattt
     3421 caaggaaatg gcgattcttc tttgaagatc gctcattcag aaattgcttt atggcgaaag
     3481 gcaggagtaa cgttcgataa ctttgaaaaa gatccggatt atttttctga tatgacagac
     3541 ttatcgatca atggaaccga cttaggtaat tttgtatcaa gccctagtgc aggtttaaca
     3601 aatgaatttg ctcagacagg tgccggaatg aaaaattata atcgaatcag tgccaataac
     3661 cagcttccga tgattgaatc cttaagagta ccaacgaatg cagataaaaa aatctatggg
     3721 catgtcgttg ttcccgaagg tgtcgaggca aagccaagag atgcttggac aggcgaagta
     3781 gaagtgactt tacgtattac ttatgcagat acgacgaaat cacctgtaga aattactgct
     3841 ctcacacaag gacaaacaga tgaaaatgaa gggacaggat ataatccgtg gggagaaggc
     3901 gaacaaggcg gtttgtttga ggttcaagta cccaacgaag aaagactaac aacgggggat
     3961 caagtgcaaa tagttgctgc taaaaaagta agaggcggcc aagaagtgac agaactggac
     4021 tctgccaaaa cgacagtcga tgtgattcct cctgagcctg ctaaattaag taccacattg
     4081 atcaatgcag cttctcgctc aatcacaggt acaggcgaag caggagcttt ggtttcattg
     4141 aaaaaaaatc aagctgtttt gtccgaaaca actattgatg atacaggtaa atttgttctt
     4201 tctatcc
//
LOCUS       DAZKOB010000162         4201 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000162 DAZKOB010000000
VERSION     DAZKOB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.168"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..1417
                     /locus_tag="ACG8M6_002825"
     CDS             116..1417
                     /locus_tag="ACG8M6_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0458406.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1444..1704
                     /locus_tag="ACG8M6_002826"
     CDS             1444..1704
                     /locus_tag="ACG8M6_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0458407.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2703..3938)
                     /locus_tag="ACG8M6_002827"
     CDS             complement(2703..3938)
                     /locus_tag="ACG8M6_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0458408.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1216 a          670 c          869 g         1446 t
ORIGIN      
        1 attatttaag agaatatatt gaaataaaat ttcttaatta tataattagt tatgaacatt
       61 ctttgcgttg tcatgcaatg aatggttcat attttattga aaaggagtgg ttttagtgga
      121 aaagctgatg aaatgggtcg aagagaaact tgtcccacca atggcaaaaa ttggtacaca
      181 gcgtcattta ttagcaattc gtaatggggt tgtttcaaca ttgtcattga ttttgattgg
      241 gacatttttt atggtcttta tcaatttacc gtttcctgga tggaatgaat ttattgcccc
      301 ttattcggca acgattgttt taccctttag gatgacgatg ggattgatgg ctatttatgc
      361 aacttttgtt atgggttcgg atctagctaa atcatatgga ttagatagtg tgacaggtgg
      421 tatattatct ttaggaacgt tctttatgtt acaagttcct gtaaacgttt taactcctga
      481 agaggcacct cttggctggg tcttacctat gagttctttg ggagcatctg ggatgtttgc
      541 ggggatctta tctatgattt ttgcagtgga agtatatcgt ttctttaaaa atcgaaatat
      601 tactatcaaa atgccagaac aagtgccacc tgctgttgcc cgtagttttg aggcattgat
      661 tccaggtgca gtcattttaa caattacttg gctgattcga agcgtcgcgg gctttgatgt
      721 aaacgctgcc ttattatcgt tattcgagcc tttaaccaat attttaggga ataatttgct
      781 tggtgtttta ctaccaatgt ttttgatcca tttgttgtgg tcatttggta tccatggcat
      841 gagtattgta ggggctgttg ttcgtccaat gtggctgatc atgttggatg agaatgcaaa
      901 ggcattggca gatggtacag ctgccaccca attaccctat atcgctcctg aacagtttta
      961 tcaatggacg gtaacaattg gtggtgcagg agcaacgatc gtggtatctg ttttgtttat
     1021 attcttctgt aaatcaaaat tcttgaaaga ggttggtcgt ttttctatta ttcctggtat
     1081 cttcaatatc aatgagccca taatttttgg agctcctatg atgttgaatc cttacatgtt
     1141 catcccgttc aacttagttc ctttagtttt gactattatc agttattttg cagtcaaatt
     1201 agaaatggtc aatagattta ctgttttgcc aacatggaca ttaccagctc caatcggcgg
     1261 ttatctttca gcaggaaatg actggcgtgt tgttatcttg atcatgatca acacattgat
     1321 agcatttatt atctattatc catttgtaaa agcttatgat aaaaagatgc tgtcagatga
     1381 acaaagtcgt ggagaaaacg aagtccagac tgtctaagtt tggtttttag aaggtgggaa
     1441 acaatgtatt ggctgatttt attttttgtt tttatctttt tattaacggc tagtcacctt
     1501 atattgaata tgctagctac atatcatatt caaatcaatc gatggatttg ggcactggca
     1561 tcatttttga tcgtgattct tccaaagatc attgtaccgc atatgaatgt tttgttttca
     1621 tgggggacat atgtactttg tggaattttt gctatcaatt ttatgatcga gcagcataga
     1681 tggtttgtca catcgaagct ttaaatgttc taaaagtggt tgtaacagaa gtggttagct
     1741 tcaagaaata agaagaaatt tacgaaaatt gcttcttaaa tttttgtgaa atgttggctt
     1801 atttttcgtt tggttgaact tattaccgca gatgcaagct tttgaccacc gtttattcgg
     1861 aatatgggac tgtgacaagg cttttgtcac agcccttttt tacataaaga ttattattgt
     1921 tttaactgtt ttggcagaaa gaaggaggaa aaaatggaaa ttttatcagc aatggattta
     1981 gaattagtag atctactacc aaagtgatca agtcaaatat agatttggga gcttacaacc
     2041 atatagtagg ggctgcagta tggtgtatga atggtaatat gtatgatgtg agtgtttatt
     2101 caaatcatga aacgtgtata gaaataattg tgctgggaat agcaatcagt catggtgaac
     2161 gtgactttta gtgtatcgtt gctgacgaca tagaacattc tgacatgatc tactcaatat
     2221 gtggggattg ctggcagttt atataggagt atgcatcgga ttgcgagatc attgtctcaa
     2281 cagaagaggg aggttgatta tcaaagataa agtggtaaat cctacatttg atttagtaat
     2341 tgttgagggg actagcggca caaatctcta caaagcgatg cttctagatc tatatgacac
     2401 attttaagaa aatgtatttg cctaattatt atggtctttc ttttgaagaa acgttgagat
     2461 gatccaacca gagaaaggtg tcaaagaatt tgactaagaa taaatggcag acgctaccta
     2521 tgctcatttg gatagcgtca gatcttttat tttaaggatg attttgtgtt ttttagacac
     2581 cgtaatttaa gtaactctaa cttttcaatc cactatttac tttttttgta ttttcactgt
     2641 ctgggtctcg aatagcgaaa ttactgatga gtctctatta aaaaagcttt ttctacttga
     2701 aactatattg ggtggttgct caggaaggtt tcgatttctt gaattgcttg ttgtggacca
     2761 tttattggaa aaacggcttg cagcgtgcgt gcctgctgac gaatcttggg tgaagaaact
     2821 tcgcgcagag caatctgcag ttttctttca gtcactggtt ttgtgagggc gattgcgttt
     2881 ccgtatttta cacaatagtc aatattacag ctttgctcaa attgcatccc cacaccgata
     2941 aacggtttgc cggattcaca ggcagtttgg acggtccctt ccccgccgtg gataaaagaa
     3001 aaatcaatga attcatgcaa aagatgacta gacagatatt ctgttattaa aagatcttct
     3061 gagtcagatt gaatgtcatc aagcagatag tcaataggtg caataaccgc tagatccggt
     3121 tgtttttgta agaagaacaa aattttgcgt aacatacgcg gattgcctga actacccatt
     3181 gcgaaataga taatacgttt tctttcttgt tgctttcgct gtataaaagt tagcacttct
     3241 tcaggcaagg aagtactgag cttagcaaaa attggaccaa cgattcgata attttccggc
     3301 agctgttctt cttccaaaaa tagtttaggc gttgtgatga gatttagatc agcatccatc
     3361 atttgtaaac ttgtttttgg taaaggaagc atttcttttt gtgcagcgtg tttgaaggca
     3421 ttggggatcc atcgggtatt tactatacgc ttttttgtcc agtgccaaag gggattgctt
     3481 aaagggccga agaaacgtaa ctcgtggggc atcagcgcat gctctgattg aaaaaaaggt
     3541 tcactcaaag catagggttt aacatatact aaaggaatct ttttattgcg ggccgataaa
     3601 aaaattgtca cattcgaacc tgtcacaatc agtcgaggct caaacttagc aatcagactc
     3661 tgttcactag tgatacgaga ggcaaccatt tctttggtaa aagggttttt aagtgtacgc
     3721 aactggtcaa attttatgat ttgttcttct tgttttttcg taaggatcgg cgctagtaaa
     3781 tgatactcaa agccactttc tgtgatcaaa ggagcaaaag ttttagaaaa gccaaagaat
     3841 acacagtaat aacggtcttt catagctttg gcaacctcaa tcattcgtgt tgtctcagct
     3901 aaattgaata ctgcaggagc aaaaagaatt gtcttcatga aatcctcctt attggaatga
     3961 gtctatccct tcgtttctta ctagtatact aaaaaacaca gcgttcgagt agcctatgta
     4021 tattttgaat attaaactgt ctctcgctgt tttcacaact tctaaattta aattttgtaa
     4081 tcaagttgaa actccaattt tcaaaaaaac acgcaacaat tttttgatcg atttatatgt
     4141 tgtgccagat aaatacgaga agttgtgtca ttaaaaataa acatgagaac ataaaaaaat
     4201 a
//
LOCUS       DAZKOB010000163         4189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000163 DAZKOB010000000
VERSION     DAZKOB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.169"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            233..1558
                     /locus_tag="ACG8M6_002828"
     CDS             233..1558
                     /locus_tag="ACG8M6_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0458409.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1551..1901
                     /locus_tag="ACG8M6_002829"
     CDS             1551..1901
                     /locus_tag="ACG8M6_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458410.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1898..2068
                     /locus_tag="ACG8M6_002830"
     CDS             1898..2068
                     /locus_tag="ACG8M6_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458411.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(2972..3247)
                     /gene="prgO"
                     /locus_tag="ACG8M6_002831"
     CDS             complement(2972..3247)
                     /gene="prgO"
                     /locus_tag="ACG8M6_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0458412.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(3219..4172)
                     /locus_tag="ACG8M6_002832"
     CDS             complement(3219..4172)
                     /locus_tag="ACG8M6_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0458413.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT         1419 a          696 c          744 g         1330 t
ORIGIN      
        1 ttttttctaa tataactaag caaaactgga taatacgaca cttattttca aattttcaca
       61 agactaatag taaatatcca tttaataaag tgatacaatg tgttgatttt acaaatcaac
      121 agaagaagta gggaaataac ttacctatgc ttcttttttt gtacatttaa tcgattcttc
      181 tttacaaaaa cgaacgtatg tttgtataat gtggaagaat ggagtgttat ttatgatgga
      241 ttattcaaaa gagccagtaa atgattactt tttgatcgat atgaaaagct tttacgcaag
      301 tgtcgaatgt atagaaagga atttagatcc attaacgact gaattagtcg tcatgagtcg
      361 agcggataat acaggatcag ggttgatttt agcttcttca ccagctgcaa agaagaagta
      421 cggtatttct aatgtcagtc gaccacgaga tctaccgcaa ccttttccta aaacgttaca
      481 tgtcgttcca ccacgcatga atctctatat taagcgaaat atgcaggtaa acaatatttt
      541 cagaaggtat gtagcagacg aagatttatt gatttactcg atcgatgaat cgatcttaaa
      601 agtgaccaga tcaatgaatc tttttacgac tgaaggaaca agaagtcaac ggagaaagaa
      661 gctggctcaa atgatccagg agaatattaa agaagagcta ggattgcttg ctacagtagg
      721 tgtcggagat aatcccttat tagctaaact tgctttagac aatgaagcaa aacataatga
      781 tggattcctt gctgaatgga cgtatcaaaa tgttcctgat aaagtatgga atattcctga
      841 aatgacagac ctttggggta ttggatctcg aatgaaaaaa agactcaacc aaattggtat
      901 catgagtatt aaagacttag ctaattggaa tccatacact attaagagcc atttaggtgt
      961 gattggctta cagctgtatt ttcatgcgaa cgggatcgat cgaacagata tagcgatccc
     1021 accagaaccg acaaaggaaa aatcatatgg taattcacaa gtgttaccac gagattatac
     1081 caggagaaat gaaatcgaac tggtggtaaa agaaatggcg gaacaagtag ctattcgtat
     1141 caggcaacat aactgtaaaa caggttgtgt ccatttatac attggcacgt cgatcttaga
     1201 aacgagacca ggtttttcac accagatgaa gatccctata acagataata caaaggaatt
     1261 gcagaattac tgtcttttct tgtttgataa gtattatgaa ggacaagaag taaggcacgt
     1321 tggaataacc tatagcaagc ttgtgtacac tgattcgctt cagctggatt tatttagtga
     1381 tccacaaaag caaattgacg aagaaaattt agataaaatt attgacaaga ttcggcaaaa
     1441 atatggtttt acgtcgattg tacatgcttc ttctatgtta gaaggcgctc gtagtattac
     1501 tagaagtaca ttggttggtg gccatgcagg cggcgcagga gggcttaaaa atgattgatt
     1561 ataatgatcg tggacgttta aagtgggcag gtttttattt atctgatcat actgaaaaaa
     1621 ttgatgccga caatgaacaa cgagccaatc aaaatttggc aaaagaacaa atgacgactg
     1681 aagaggttgc ggaagtattg aacgctgcag tcataaaaaa tagatcagta tgtattcaaa
     1741 aagaagaacg aaacgctgaa ggatactatc cagaagatat tgttagcaag atcttaggta
     1801 gtgatgagct ggggatctac gttggaaaaa acaaagttca ctacgacgaa atacgtcata
     1861 tcgaatttta ccaggataat aaatggggtc gattagaatg aacattatcc agcaatatga
     1921 gctaaagtat ataacctttg atcagctatc agaagagatt tggggttatg gccagcgttt
     1981 gataaatgag gttggtgttg aacgcttctc tttctatgta gaagctgcag caggatacca
     2041 taaatacgaa tactatattg gctattaaag tgcaataaaa ttatatttct tttatgtttt
     2101 acattttgta tcaattctga taaaattacc tataggtaat taaaaaatat aaaaaaacta
     2161 gttacaacct taggacgttg taactagctt gaagcatact agagctaact aaaatagcac
     2221 tagaaagcaa tgttaaaccc gtccaacaaa cttgatttaa cattgagacc agcttattca
     2281 gctaaatata gttgaataag cttcttttgt tttaaaaaaa caaaaggtgt actacgaata
     2341 acgcaagtaa aagaaatact attacaacca gtaggctaca agtaagtatt actaaacatt
     2401 gcgtagttag aacctttgct ctattgtcta aagccacaaa attagagatc agctttatga
     2461 tgctaagagc gagctgcatc catgcggaca taggttcttt tgacactatt aattctcctt
     2521 tttaggataa gggaaattgc tagtagtttt ttcattagca ccatccccct tcatttgatt
     2581 gagtctagtg ctgctgtgat agtttgctgt ttaatatgct tacattctta ttataacata
     2641 aatgaaacaa acacatggtt agaggtcgta tcaatcaaaa tagtgtctca caaacgattt
     2701 attacttact cctgctgact aacggactgt tcttcagtaa ctgtaactat atgtttgtta
     2761 aaaccaccat tacctgtaga taaaaaccaa tatatttact atactcacta tattaaatat
     2821 atttagtata gtaaaaacta gaaacaataa aatttgtgaa tgttgtatac ccccaaacaa
     2881 tacacatatt ttgaaggatt aggattaata aagctaaaaa tatagtaagt atactaaata
     2941 tatttactat atctagtata cttactatat tttaatcttt tgatcgtcta ttatatataa
     3001 tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat tgcaagtttg actatttcat
     3061 acataggaat atcttttgca taactaagat cccgaatggt ttttagtacg ggaggatcta
     3121 cttgaactgg ttttctttct ttcgcagtgt actttcgttc ccctaactcg acattaatat
     3181 catctttaat tgaaatatct tcagcaccaa ttttcttctt atctttgttc gttggtaata
     3241 atgccattta gtttaatctc ctttccaaga ggtaggatat gattggccat tgagtcagca
     3301 tatttaggtg tataatcaaa tccttcaata tcaccatgtt gttcatagta ttcaattctt
     3361 tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc atcttcgatc tacataatca
     3421 tttaattgga taccattctc accatacatt tctaaccgat ccgagccttt aacaatgctt
     3481 ttaaaaatgt ttgattcacc atacttatca attgtttttt gatagtttgc gtgttgagag
     3541 gaacgtcgca ctgagaaaag aacaggcaaa attccaatga tttgaagatg agactcttca
     3601 aaattcgtga caatcggaac taaaacttta ttaataaaat cttctgttcc ttccattgcg
     3661 taccgtttta attcttgcat tgcaataata taatctgcag cagttaagaa tgcacgaacc
     3721 acattatctg ttgatggtgg gcagtcgata aagatataat cgaaattttt tctcaattct
     3781 tctaaaggtt taatgaaggc caatgatctc ttacgacgat caggatattt ttcggacaag
     3841 tattcattaa gttcttcaga tccaggagag ccagccataa tatataaatg tggacttact
     3901 tgagtaatac ctgaaaccca gtcccctttt ttgattgctg cagacatgga tcgatcaaag
     3961 ctagtaattt gatacgttga tccaatgttg gaagtagtat ttctttgttg gtcataatca
     4021 attagcaaca cgtctttttc aaaaagttga ctagaaacaa tcgctaataa atcattgtct
     4081 gtagtcttac caatgccgcc ttttctacct gtaagcatta caacttttcc tagtttatct
     4141 gatgttttaa atgcagtccg taagttttcc atagttccct ccaatttcc
//
LOCUS       DAZKOB010000164         4074 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000164 DAZKOB010000000
VERSION     DAZKOB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.170"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..1688)
                     /locus_tag="ACG8M6_002833"
     CDS             complement(183..1688)
                     /locus_tag="ACG8M6_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0458414.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1685..2371)
                     /locus_tag="ACG8M6_002834"
     CDS             complement(1685..2371)
                     /locus_tag="ACG8M6_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0458415.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2564..3985)
                     /gene="gndA"
                     /locus_tag="ACG8M6_002835"
     CDS             complement(2564..3985)
                     /gene="gndA"
                     /locus_tag="ACG8M6_002835"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0458416.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1123 a          859 c          694 g         1398 t
ORIGIN      
        1 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
       61 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      121 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      181 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      241 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      301 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      361 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      421 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      481 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      541 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      601 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
      661 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
      721 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
      781 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
      841 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
      901 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
      961 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1021 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1081 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1141 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1201 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1261 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1321 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1381 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1441 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1501 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1561 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1621 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     1681 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     1741 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     1801 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     1861 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     1921 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     1981 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2041 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2101 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2161 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2221 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2281 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2341 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2401 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2461 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2521 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2581 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     2641 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     2701 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     2761 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     2821 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     2881 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     2941 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3001 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3061 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3121 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3181 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3241 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3301 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3361 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3421 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3481 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3541 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3601 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     3661 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     3721 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     3781 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     3841 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     3901 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     3961 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4021 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAZKOB010000165         4032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000165 DAZKOB010000000
VERSION     DAZKOB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.171"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            569..2062
                     /locus_tag="ACG8M6_002836"
     CDS             569..2062
                     /locus_tag="ACG8M6_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0458417.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2197..2493
                     /locus_tag="ACG8M6_002837"
     CDS             2197..2493
                     /locus_tag="ACG8M6_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0458418.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            2594..3445
                     /locus_tag="ACG8M6_002838"
     CDS             2594..3445
                     /locus_tag="ACG8M6_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458419.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            3466..3678
                     /locus_tag="ACG8M6_002839"
     CDS             3466..3678
                     /locus_tag="ACG8M6_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458420.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1434 a          584 c          807 g         1207 t
ORIGIN      
        1 atatacttaa tatattcgat atatctaata tactccaaat tgataagaat tcagacttta
       61 taaggaatat agtggatata ctaaatatat ttgctatatt cagtatatca gatatattca
      121 aaatgtcaaa atatatttgt gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact
      181 taattctata gaaaccacag ataattcact atttacaaag tagcaaaagc agtggtactc
      241 tttaaacaga aaaattacat aaaaaaaaga gacggaaata attgatttta gcagaatcaa
      301 ttatttctga atcacatggt taggacatgt gttcacgctc gtctcaagaa tatgtaagta
      361 gctaccaact acttatatta aaagttgatc ggataccttt tccgattgcc tttattctac
      421 caaaaaaagg cgagcaactc aacagaagaa cgtttattta gtgtacttaa attaaatatt
      481 cttaaacact gaagagagaa cacatactat ggctgagatt aaagctaaac tgtgttctct
      541 ttttgtttga gaaaggacgt tacaatcgat gttgagcgaa atttattcag ctattcttaa
      601 aaacggctta cgtacataca aatatcgaaa cagcaccatg aaacctgcag gtcatattga
      661 ttccagtagg agtggtgcta tctttgcgta ccgtagcaag caattaatga atgttgggcg
      721 tggtatggtc attacttcgg aagaagctat tttagagaac gaaaagaaac taacgcattg
      781 gacacctaat acctatcgct ttggtactta tgcggatgat tatcgaacag tggttaaagg
      841 tcattctgag aaaaacttaa gtcaaatcaa cacatttgta gttgatatcg attcaaaaga
      901 gaaccatcaa ggcgaaatta ttttagcctg tttagatcaa gtgggctaca tgccaacgct
      961 aattttagaa tcagatcatg gttatcaagt ttattttgta ctaaagacac caggctatgt
     1021 cacaaagaaa agcaatttca aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg
     1081 tctacagttg gctgaaaaat tgtccggagt ggatctagga tgtaaccact tcggaattgc
     1141 tcgattccca caagaaaata atattgtttt taaagaaatg aactatcagt attcttttgc
     1201 tgactggatt caatggtcga tgaagcttga atcagatcaa aaaacaaaag aagatcgaga
     1261 acgaaaacta attgtctttc ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt
     1321 cgatatgtta cttcataaag caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa
     1381 tgtgattttc acgctttctt tagcttatta ctcttcaggt tatgcattgg atacttgcga
     1441 atacaatatg ttcacattca acgaacggtt acaggaaccg ctatctgaag cagaattagt
     1501 aaagattgta cgtagtgctt attctggtaa ttatcaagca gcaagcagag aattcattat
     1561 tgaattgtgc cagcaatggg tttcaccaac cttaaaaaat aatgatctgt tcattcaacg
     1621 acgtggctgg tggaaattta aaaagccacg agatcaaaga aaatatagtc ataaaaatga
     1681 atggaaagaa gatctattgg cttatctaag cgaaaaaagt cttgctagtg agccttattt
     1741 gaacctgtca aaaaaagaat tggcttcaca actaacgatg cccaagcgaa cacttgataa
     1801 gatcttaaat gagttacgag aggagaataa agtcttctac aaggtaacac gaggaagaaa
     1861 tggcggaatg atcattgctt cagtcaaaac gttgttcgcg agggttattc aactaagtaa
     1921 agaaaagaga ctagattatt caacaaggat cacagaggta tttggactca acgagagctt
     1981 agtagaagag acatttagac aattatcaac ccccttgaac cctcttaaag aagtgaatct
     2041 ttttgaagta gatacaggtt gaaaaataaa aggtgcaaat tgccctatac aatatatcta
     2101 tgatacttct gtaggtgttg ctccctttgc ctagctacgt gtaggtggta tacttataac
     2161 tgcgtatgca gtcttatgta tcatggagga agttgtatgt cgttaaaaaa ttatgtctat
     2221 tcacatcctg ttaatgtgtt tatcattggt cgtttgggat tatctgtgga agtgttctgt
     2281 gagctttatg gttttaagca ggggactttg tcttcttggg ttaccaggga aaaaacagtt
     2341 gcttctttgc cgattgagtt tcttcatggg ttgtccttag cttctgggga gactatggat
     2401 caggtatatg attgtttgtg tgtgttagag caggagtata ttgagtataa aatagctaac
     2461 gagttaagaa aaaggaagaa gtatatccag tagtagtttt tatttactta aaaaattggt
     2521 tcattgagca acctatcgct gctaaagtag caaaggaaaa cgttaaagga aagaatttag
     2581 ggaagaaatc gaaatgaaat cggacgactg gattgaacac acctggcttc ctctgaaaag
     2641 aaatagcaaa cgaaagaaat tttgggtatt gcaattatgg ccattaggaa aactttgtag
     2701 ttacggggca cgacttattc ttgatttaac cgtttactcg attggagtat ttggaacggc
     2761 ttattacttg ataaatgatc ccaataggat tgcagcatca gacattttta cagtgctaat
     2821 gacgttgatt tttgtcatgc tattgtctat ttttactcaa ttagtcatgc aatttaccag
     2881 gcaaaaatta acagcaggaa aacttttcaa aagcctattc gatttaattg ttgtaaatgc
     2941 aattctagcg actttaataa aacaaattga acaatctgga agaacaacaa aaggagtgaa
     3001 ggatttttta ttgacgttta ttcactttgt aaatggacac atgtatttgt ttgaagtggg
     3061 tattattctc ttttttgtcg ggtatgtatc cacgaaactt attaaacgtt caattgaaaa
     3121 agaaattgaa attgacctga ttccgacact tcttggttat gagtacaatg ttgattttgg
     3181 aagatatgac gaattgatca atacggatat cgttcacacg agtatccatg aatctactgt
     3241 taagggcatc agatctattt taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa
     3301 agagatctac aatttttatg aagtcgaagc aagcctacga tatggcatcg gaaaattgtg
     3361 gttagatgga agtaatattc gtcaaaacaa accatacatt ttttggaaca gtagtgattt
     3421 agaacttcca atgaaagata aataattatg gagaatcgtt tgaaaatgat tcctctatac
     3481 aggattgtgc ttatagttgg catggagtta aaaaccttaa agaaggagca gttattcaaa
     3541 gtgaaagaac gtcataatca gttactatgt agtaaaacgg gtacgatgaa cattgaatac
     3601 cgaacagaat atttagaatt aacgaatcaa actgattttt tgttgattcg aaagcaacaa
     3661 gagcaagaaa aaaattaatg gctacttata gccaattgat tgatgaatgg gaacaattca
     3721 aggaatctaa aattccagaa cgtctctaaa agcctttatt tgtaaagagc ataaggaaac
     3781 gtgggaacac tttttaaaaa aagtgttcca ataggctgtg cctattacgt ttctcaaaaa
     3841 taaatgtggg aacaaaatat ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa
     3901 aggagggtag taggatagca aaaaggatag gctccttttc gctatccttt ttaaattgga
     3961 ggaattaata tggaagtaag aataagaaat gtggatgaga aaatcgtaag agaaattgat
     4021 cgaagatgtg ac
//
LOCUS       DAZKOB010000166         4007 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000166 DAZKOB010000000
VERSION     DAZKOB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.172"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..369)
                     /locus_tag="ACG8M6_002840"
     CDS             complement(220..369)
                     /locus_tag="ACG8M6_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458421.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            831..1043
                     /locus_tag="ACG8M6_002841"
     CDS             831..1043
                     /locus_tag="ACG8M6_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458422.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1321..3120)
                     /locus_tag="ACG8M6_002842"
     CDS             complement(1321..3120)
                     /locus_tag="ACG8M6_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0458423.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3244..3840)
                     /locus_tag="ACG8M6_002843"
     CDS             complement(3244..3840)
                     /locus_tag="ACG8M6_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0458424.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1437 a          710 c          688 g         1172 t
ORIGIN      
        1 taacggaata ttatcatcga aacagctaac aaaattgtca taaaaaacga gatggttgat
       61 attagaactt gtgtttttgt ttaggtctag agataaagag agacaatttc caaatatatg
      121 taatgaatac aaaaagacgt ttgtcgcgga ttaccgttac aaacgtcttt tattgatttt
      181 ccgagatctc atcagtcgcc atttggaatg gttcactcct tatctagatt cgtttaatgc
      241 tcggactgct tgttcgattg taataccaaa cgcaacgtta ttttcgtttt gtgcatcaat
      301 cgccataaaa cggttagaag ttgtatcaaa agctacgcgg tattgcatat ttgtttttgt
      361 aaacgtcata aaattcatct cctttgtaat gtaatttagt tcttgaaagt ttcctttaaa
      421 ctttcgaact tataccaagt ttagcatcgt acgttttttg atgcaattaa aaagcattta
      481 tcattttcct tatttatttt ttcttaaatg gtttagctat tttcttttaa tccgataagt
      541 agcccaatca tcaaggttac aaatactatt gcttgaatat aaatcatatg ctcacctctt
      601 tataaataac ttagcaatat aaagaaaaaa acgcacgaac aacataggaa tataaacaaa
      661 cgtttttgtc acaacgtcta agatcgtgtt aaaaaatagt tatctaaaaa gcagaaagat
      721 actcaaaata aaaaataaaa aaatggataa aaacgttttc aaatacaagg taatttgtta
      781 ttataaaata aagaaaaaac aaaataaaaa taaagaaaag agggaatgaa atggtaaaag
      841 cagaagtagg aagatcttat atatgcaaac cgatcggagt atctcatgaa ctaatcggct
      901 gtattgaacg tacatatatc aacacggctg tcgtgtatgt tgaaagttat gaccaacgag
      961 actatcaact tatcaatgaa agcaagtatc gcatgctcgt aaaattctgt gatattagcg
     1021 cacctgctga attggtttct taaacagtat ttttgagaat tcgcaagaac tcccttataa
     1081 tataaataaa gacagaaggg agcgagaaga tatgccaaaa agaaaaagtt cagggaaaat
     1141 gggaattatt cttgcagtga tctctgcaat tcctgctatt ttagctattg ttcgtgcgat
     1201 gaaggaatca agagaaagtt aagcacacat tatgagcgtc aaacaaaaaa cgcatatacc
     1261 tgcggtggca ggttatgcgt tttttgttgt ataaagactt ttttcattaa cgatttttga
     1321 ttagccgact cccaaaacaa aatttagcag tttatctgaa taagtgattt cttcatctgt
     1381 ttgtttcaca ctttcattta gtagtcccat ctcatcggtt atgataccat ccacaccata
     1441 aaacatcatg cgactcatag catctgaatc gttgattgtc catacatata cttcttttcc
     1501 gtcttggtgg gcagaattaa tgaagttacg gttgatcgta ctatattcca ttgtcagaaa
     1561 atcagcaggc gtcacagggg gcccaacaat attgaaaggt aagatgtagc ccacataaaa
     1621 gtttggttct gcttctttta aatctgaaac ggtttggtac gtcaaacttt gcaaaatatg
     1681 atggttctct aagataactt tgcggtattt atggacaaat cgttcgacaa ggtcatccga
     1741 atctttttta gaggtcttga tctcaataag taatttttgg tttagcttat gtgctttttc
     1801 caaataagaa tcaaaagaac aaacaggggc ttgcactccg ttttcagtaa ctgtaagatt
     1861 tgtcaattct ttcaatgtaa gatcctgtgg tgttccattt acccctgtta agtgacgaag
     1921 atttgcatca tgcatcacta cgaaatgacc atcttttgtt tcctgaacat ccatctctac
     1981 ataatcagga tgatagtctc tgcttgtttt ttcaagtgct tctaaagtat tttgcgcccc
     2041 attttgggta ctcacacctc tgtgagagac agtcactggt tttgtctgaa cagctgaagt
     2101 cagataatcc atattgtaaa gacagacgcc aataccgaaa aaaactgcaa aaagagtcaa
     2161 accagtgttt ttcagcgcag agaaacgcaa gttgggggct tgtgggataa accacttagg
     2221 gatctcaggc aagaagcctt cgtcatccat gaaatcaaca atgatataaa aaatccctac
     2281 ggtagacatc acgatattga ataataaaat cccttgtaaa agcgtcatag caaacacagc
     2341 gctgatcaat gcatagtctg ggaaaaattg ttcaaccata ctttgtgcca aaataacaac
     2401 gatatatcct aaagaagaaa ggagtaaaat cgtaccgcca atcacaataa attggcccgt
     2461 gatggccaaa agacgcgatt tggtcaatga ccagctttcc cgaatggctg ctcgaaaagg
     2521 acgatctcgt agaatcattt ccggtaaagc aaaaatcagt cgaatcccaa tatagccaag
     2581 gaaaatataa acgagtaaaa aggaactgac gatgatccaa cgattagcaa aaataaaatc
     2641 cataatgaaa gcggggattt ttatttttga cagtagatca gaattaaaac ttaatccgct
     2701 gatcggtaga atcaataaaa aataagcaag gaaaaacaaa aaagtaatgg gtctcacttt
     2761 cttcagctga agtatcgtca agtgtaagag ttgcttcaaa gagattggtt ctttcttttt
     2821 aataaaaaaa acactcatca aaagaaaagt aaattcgaaa taaactaaca gtaaaatcgt
     2881 catcaaaact acgattaacg agagtagaac cccaggatgt tgagaaaaaa ttgaaggaat
     2941 attgtcataa gacaaatagt caattgcccc tcggtgtaaa ataaaacggg tcgtactgct
     3001 caataagggt aaacaaataa ataaaataaa gccatgcatc agcaagacat cacggaaata
     3061 agcttgtgtt ccttttaaaa agtcccaagc attacctaag ctacgtttca aataattcat
     3121 cctatcacct gctcttcaca ttgtagcgca aatctaatga aaaaggaaag ggtgcagtgt
     3181 gttttgcaga ccctttccca gatttaacat tgtcaatcaa tgactaattt tacaaaataa
     3241 atattatcca agaagaccac ttagatattg acctccataa atcaaggcca tactatataa
     3301 gaaaatcgac agtggtttag caatcaaaat gattagtgta tatcgtttca ctgacatgtt
     3361 tgtcaatcca gccatcaaac ataacgcatc gtcaggagca actggaagaa aaatagctag
     3421 agcaaacagc ttctcaaaac ggttttgatt atccagccag ccaatatatt tgttatatgt
     3481 cttatcgcta atcatggaaa gtatgaatgg ctttccataa cgacgcccta gcagaaaaat
     3541 gataatcgaa ccaatgcaga ttccaatata gttatagata aatccagcat aagggccaaa
     3601 tatcaggaca ccagctgcag tactgatccc tccaggaatg attggaataa ctacttgcag
     3661 gatttggatg aagacaaaaa taatcggtcc taaaatgatg gaatcaccta ctaatcctct
     3721 taacgcattc aaatctttga aaacacctaa attaataaaa taaatcgtca gcgcgattga
     3781 taggcctaat ccgataatgg atatgaaatt tatcagtttt cgagaggcag caatattcat
     3841 tattcatcaa ctccttagag gttctttgtt aactatactt tactagaaaa gcaactaact
     3901 gcgccatttt ttgatatgac agaaaacaaa caattttgta agttcatcat aagtataaaa
     3961 gattgatctc aaaaaaacat ccaactaaag acttatttaa taacttg
//
LOCUS       DAZKOB010000167         4002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000167 DAZKOB010000000
VERSION     DAZKOB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.173"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(442..615)
                     /locus_tag="ACG8M6_002844"
     CDS             complement(442..615)
                     /locus_tag="ACG8M6_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458425.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(618..1016)
                     /locus_tag="ACG8M6_002845"
     CDS             complement(618..1016)
                     /locus_tag="ACG8M6_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458426.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1232..2287
                     /locus_tag="ACG8M6_002846"
     CDS             1232..2287
                     /locus_tag="ACG8M6_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458427.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2307..2660
                     /locus_tag="ACG8M6_002847"
     CDS             2307..2660
                     /locus_tag="ACG8M6_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458428.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2842..3129)
                     /locus_tag="ACG8M6_002848"
     CDS             complement(2842..3129)
                     /locus_tag="ACG8M6_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458429.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3142..3960)
                     /locus_tag="ACG8M6_002849"
     CDS             complement(3142..3960)
                     /locus_tag="ACG8M6_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0458430.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
BASE COUNT         1365 a          705 c          574 g         1358 t
ORIGIN      
        1 gttatcgtaa aatggtataa aaaagctaaa tattctattt ttgttatttt attagcaggg
       61 aaatttaaat agtttacaag aatccgtaga aatatattgg ttttgccttc agattaatac
      121 tggatcatat taatctgaat ataatatatg aactaataaa tagcttaacc tcgctatttt
      181 gttttttctg atcactatct atacagcatt agtaaataga attttaaaaa taaaaaaatt
      241 aagacaaaaa aagtatcaac ataaacatat acatatacac gtttatgttg atacacttaa
      301 tttgtccttt aaaaagcact tattttaata ttatgttatt ttataatctt attacaagct
      361 gattgttaga cgttgtcctt tcattacatt tattaacggt tattttatcg tttaatttat
      421 taattatttt tgatctaaaa cttagcttaa caaataaata attaaccaac cgccagataa
      481 aaatgcgatc attgctaata cataagcaaa gataaaaata tgttcgatca gcttaaaccc
      541 tgatgcgatt aagcagacca agtaaaatag aaattctctt acagataatg gtaattttct
      601 aactagcata ctcataatta acaccccaaa gtaatattgt attttgaaca ttttacatcc
      661 aatacagcat caatcaacca ttgccaatca aaaatacaag gcttcattga agtatgtttt
      721 tcagatgaaa agatataata taatttcttc ggaataatat tttcagttgt tgggatcaag
      781 ctaaatttaa taccatctaa ctcatagcta ccagctttct ctccattaaa aatttcctta
      841 gcactttctt ctaaagaatc aaaatatttt tcgacagcaa cctttgaagc ttttactatt
      901 gaagcatttc ccttttcgat aaaatctgct tttgttttac cgacaataag taattcttga
      961 tcagtaaaat cttctaaaat aatagtagtg taattcttat ttcttctcaa tcgcatcagg
     1021 atcactcctt aatataattt atgcttttat aatatcataa taagataaat ttgcaagtgt
     1081 ttatttacac ctttttattt acactttatt gtaattgatt atactttttt gattgcattt
     1141 ttttcttata aacagttgca actattagta aataggtgta aactattttc gtaccgaaat
     1201 accataaaca caaacagaaa gaggtcatac catgaaagaa ccattattgt tttccgttga
     1261 cttagggaat aaatcagtaa aagaaatgag tacacacgtt tctccactct cgcttccctc
     1321 tcgtattttg aacaaaaata tcattaattc aggacgtttt tctaatatag gttcctcatt
     1381 aaaacgatca ctaaacgttt ctgaatatag cctagtaaac actactgaaa gctattattt
     1441 tgggaaggat atacacgagc taggaaaaga tgactacgtg atagaatcac taggttttgg
     1501 gatcagcaga tacaagcagg aaacctacca agatatccta tctttctctt tagctaacct
     1561 agcacgttta gaagatcaat ctgatgacgt tttaaccgtt aatctagttt taggtctgcc
     1621 ttcatccgac tataccgaaa caatcgtcaa atacgtgatg caacaagcca cacgacaaca
     1681 ttcagttgaa gttgacggta aagcttataa tataaacgta gataccgttt gggtactgcc
     1741 acagccgatt ggaacactat ataacctcct ccttgctgat gatggtagtg tacaagatga
     1801 atcattgatc agtcagaaga tcggttttgt tgatattggt ggtggcacga aactttttga
     1861 tgttgtccaa aattttgaaa ttgacgaaaa caaccgtgaa caaaaggata ccggtatttt
     1921 tactttatat aatcgtatcg taaatatgct tgaccttccg atcagtccta atgctttcca
     1981 acttgaaaag attatccgag aaggcaaaga aaaaggcgta tatctattta aaccaagcgg
     2041 aaatgaaagt tttgatattt cagaagtagt tgaaagagaa ataaaaacat acaccaatat
     2101 ggttgttgca gaagttttag ctacattcaa aaacttcaca accttagata aagttgtctt
     2161 tactggtggt ggggcttcta ttatcgacaa acaacttgta aaagataaaa ttccgcatac
     2221 cttgtttgtt gaagacagtg aattttctaa tgtaaaaggc ttttacaagt ttggtttaac
     2281 tcaataaaac agcgaaggag atcaacatga ataagggaaa atcagagcgt tatgtagtta
     2341 taatcaatag agaaaaggtt agcgagttac ctttagcaga gtttcttgaa tctgaatatg
     2401 aaacactagg cgttaaatac tctcagacga tcaaaaaagc tttgaatcta tatcggcgtg
     2461 taaagaacaa tgaagttaca attattgaag caggcaacga tagaaacgat agcaaagtag
     2521 ctgaagtaaa gaataaacca actgatcata accctattca acaagcgcat ccttcaacgg
     2581 ttacatccga tcattcagca aagaaagaaa agccaatgct aaaagggttc aatgcaccaa
     2641 gtaacaaaga cttactttaa aaataaaaag gcgaggttac aatacctagc ctttttatta
     2701 tgtacaaata caaacataaa catatacgta tacatgtata taaacatgta tacgtatata
     2761 tccataaaca cgtatacgaa atttagttaa gctataccta accgcttatt ccaataaaaa
     2821 aagtgatcag ctttaaactg atcactcacc aaaaacttca tcatacaagt tatctatcat
     2881 ttcaccaagt aaagctgaat ctgatcgaat tcctaacttt tcgctgattt tagatagttt
     2941 ctttctttgt gatggttgaa gcgtgaattg ataatttttc ttcttttcag aactatcact
     3001 ttttagttta gctaccaaag aacttgttgt ttctttaggt gtttgtttag gttcttcaac
     3061 tgcttcaacc acttcaggaa cttcttctac ttcagcttca gcgaattttt taaatctatt
     3121 ttctttcact tcaatcactc cttattcttg tgccgtatca attattttgt tgtatagcgt
     3181 aaacgtgctt tcaaagaaat ccttattctt acgatagata ttcttatcct tagcgaactc
     3241 agcaatattc ttaccttcta agctagttgc attaagcaat tccttctctt gtacatatcc
     3301 aagataatta gataacgttg gtaccaattc taggaactca cgacttgaat tagtattatg
     3361 tttgatcctg ctaccgatcg tagcgtattt agtttccatc agtgtttcac cagtcttaat
     3421 atcaatcaac tgttttgcta gtgcatcaat atctttacca agcttaagca aagcttcaaa
     3481 accatcttta gatggatcag aaatgcctac gacaagatca gatacagccc aagcattttg
     3541 agctagtaga cctgcatcat tatgagtatc aatcagaatt agatcatact tctctttaag
     3601 ctgttcatag ttaacaacga tccatctaaa cagtagggtt ctgttgttag gcatatcacg
     3661 catttgcatc tcaacttgtt ttaaatcttc acctgctact aaaatatcca tgttctcatt
     3721 tatagctaaa ggttcagctg atccatgaag atataaatga ttgattgtat tctctctttt
     3781 cgctttactg aaagcatcag gaaatcgttt tgataggttt tgcgacttat cttcatcaat
     3841 atacagcacc ttcataccta aattttcgac agcatatcca cctagattgt agattgttgt
     3901 tgttttacct gttccacctt tttcaatgtt tccagtaatg attgacaact tcttttccat
     3961 gattaaacct cccttattaa tcttttgtaa ctccattata ca
//
LOCUS       DAZKOB010000168         3883 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000168 DAZKOB010000000
VERSION     DAZKOB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.174"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACG8M6_002850"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACG8M6_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG8M6_002851"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG8M6_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="ACG8M6_002852"
     CDS             2090..3130
                     /locus_tag="ACG8M6_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0458431.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT         1416 a          541 c          579 g         1347 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataatagcta ttttaataga
     3241 atttataagt taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt
     3301 taaaagaaat aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt
     3361 tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct
     3421 tgatattact tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt
     3481 aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt
     3541 ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc
     3601 gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac
     3661 ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg
     3721 ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat
     3781 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
     3841 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tca
//
LOCUS       DAZKOB010000169         3863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000169 DAZKOB010000000
VERSION     DAZKOB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.175"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACG8M6_002853"
     CDS             complement(245..466)
                     /locus_tag="ACG8M6_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458432.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACG8M6_002854"
     CDS             complement(564..809)
                     /locus_tag="ACG8M6_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458433.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACG8M6_002855"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACG8M6_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACG8M6_002856"
     CDS             complement(2033..3034)
                     /locus_tag="ACG8M6_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0458434.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACG8M6_002857"
     CDS             complement(3193..3459)
                     /locus_tag="ACG8M6_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0458435.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACG8M6_002858"
     CDS             complement(3449..3805)
                     /locus_tag="ACG8M6_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0458436.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAZKOB010000170         3834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000170 DAZKOB010000000
VERSION     DAZKOB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.176"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..540)
                     /locus_tag="ACG8M6_002859"
     CDS             complement(<1..540)
                     /locus_tag="ACG8M6_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0458437.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            675..1064
                     /locus_tag="ACG8M6_002860"
     CDS             675..1064
                     /locus_tag="ACG8M6_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458438.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1068..1832
                     /locus_tag="ACG8M6_002861"
     CDS             1068..1832
                     /locus_tag="ACG8M6_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0458439.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1961..2908
                     /locus_tag="ACG8M6_002862"
     CDS             1961..2908
                     /locus_tag="ACG8M6_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0458440.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3038..3778
                     /locus_tag="ACG8M6_002863"
     CDS             3038..3778
                     /locus_tag="ACG8M6_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458441.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1266 a          663 c          791 g         1114 t
ORIGIN      
        1 ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat attttggctc
       61 ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca gttcacgttc
      121 gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc ccattgcaga
      181 agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt tatcttctgc
      241 gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt tgtcttcaat
      301 caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata aattcagtgg
      361 attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga tctcgaccat
      421 actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac gtaacgtgtt
      481 cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg gatcaatcat
      541 ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa gtgaagaaaa
      601 attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt ataaagatag
      661 gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac gtgctaagtc
      721 tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag gtagagaaca
      781 ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc tttttgatac
      841 tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct atttatccac
      901 tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt cccgagccat
      961 tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact gttggagcat
     1021 ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg aatccaatta
     1081 tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa gaattaagag
     1141 aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag gagttagttt
     1201 tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca gatgcgatga
     1261 atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt tgggatagta
     1321 tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa agaggtcttc
     1381 tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca tgcgccttgt
     1441 tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct gcacctttgg
     1501 aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta ccaaaagact
     1561 attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg gaaaagttag
     1621 ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct tatatacaaa
     1681 cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt gtttttcgtt
     1741 cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct tccacttttt
     1801 tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact catgacgata
     1861 gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa tttggtataa
     1921 ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt tagcagatgt
     1981 tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc atccggaaca
     2041 ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg attaccgtcc
     2101 aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat tatttatact
     2161 ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa tcgctaaacg
     2221 gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg acgtcggttc
     2281 ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga tcgaccgttt
     2341 ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg tagacagcga
     2401 taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt atcaaacgat
     2461 tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa ctggttattt
     2521 atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa atcattcgac
     2581 agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa cttgttttgt
     2641 atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg ctggaggaca
     2701 cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc aaatcagttc
     2761 acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat gtggagaaat
     2821 gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt tttttcctga
     2881 actagttatc agagagacaa cgaattaagt cttattacta tatatatatt tatgaaattc
     2941 atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat ctgtgctatt
     3001 attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca
     3061 tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg
     3121 agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc cgtttgaaca
     3181 gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc
     3241 aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt
     3301 accggattat accggccatt accaatcaga aatcacatcg acaggacgaa aaaaactttt
     3361 tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca agcatactac
     3421 acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt
     3481 acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc atttaatcga
     3541 atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga cgccgtatca
     3601 agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa cgttagagga
     3661 aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt
     3721 acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt ttaaataagt
     3781 gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt ttcc
//
LOCUS       DAZKOB010000171         3764 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000171 DAZKOB010000000
VERSION     DAZKOB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.177"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1070)
                     /locus_tag="ACG8M6_002864"
     CDS             complement(<1..1070)
                     /locus_tag="ACG8M6_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFQ0458442.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFA"
     gene            complement(1074..1484)
                     /locus_tag="ACG8M6_002865"
     CDS             complement(1074..1484)
                     /locus_tag="ACG8M6_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0458443.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1502..1993)
                     /locus_tag="ACG8M6_002866"
     CDS             complement(1502..1993)
                     /locus_tag="ACG8M6_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0458444.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(2003..2794)
                     /locus_tag="ACG8M6_002867"
     CDS             complement(2003..2794)
                     /locus_tag="ACG8M6_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0458445.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2787..3560)
                     /locus_tag="ACG8M6_002868"
     CDS             complement(2787..3560)
                     /locus_tag="ACG8M6_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0458446.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1171 a          788 c          511 g         1294 t
ORIGIN      
        1 aaagcaaact cctttgttaa cgatgtaata gtcaaagttg tttcaaatag aaaataatct
       61 tcgattttag gaacaattac tctttctttt tttccaaatt ctttctctaa cttaaaactt
      121 gaaaatggag tgatttcttt aaaattctca attatttttt caggcagttt aacaccaaga
      181 gttttgtctg tcttttgaat aatttgatgt ggaacaagtg caccgcccca atcccagtta
      241 ttttgatcgt tctccccttc ttttgttgcc acccagccag ttagaaaacg gccagtttca
      301 ttagttccga ctgttcttgc tgcataatag gcttttccgt cgaaagcttc gtctactggt
      361 gatttccaat ctgaaaataa tcctgctccg actcgatatt tagtcttttt atcttcacaa
      421 tactctgaaa aaagtaaata ccacgttcca tcaatttcaa atatatcagg catttcaatc
      481 atgttaaatt cattattagc ccagtagtct cctttgaaat tccaatcttt taaatcttcg
      541 gattcaaaat agaccaatct ccccgtcggc tttttcttac ttgctggttt tctagctcct
      601 agtatcaaaa cgtattttcc atgatcttga tcaaaaattg caaaaggatc gcgccaatca
      661 ttcttatcat atccttcttg aggaacaagt tttgctgctt cgccatcctt tttccaatga
      721 attaaatctt tagaacttgc aatcattaac acttctgaag tttttccatc caacttagct
      781 ttgcgattat gtccagtata caaagctaaa tactcattga ttccttggac aatacttcca
      841 gcgtaaatgt attgatcaat gtcatcgttg ccaccacgat gaattacttc cccgtattct
      901 tcaaatttca caaaatcagt ggttgtaact agtgaccacc caaaaggatc agttaaaggt
      961 ttaggatttc tcgtatctcg ttgatgaaat agataaaaag ttccatcttt ataaaaaggc
     1021 atgcaatccc caaaccaaaa atctttaggc ttgtaaaaaa ttttttgcat ttatcactca
     1081 tcctcttcgt tagtatgtct ttgcatatct gcaagacacg caattgtatt catatttctt
     1141 agattttcga tgatatcatc gctttttttt ccttgtaatc tcaaattgac agcctcaatc
     1201 aaaattccta aattcatgcc accaagaagt tgaaacccat tttgaaatac ttcacttctt
     1261 ttagcaaatt cgttgaaagg tgttccacca atcaagtcag caaataccat aacttcttga
     1321 ttttttactt gagaaactat tttttcaatg ttttcacgga attcttcaat gcctgcatct
     1381 ccaagtgtta ccacatgaca atttaaatta tcgccaaaaa acatttttaa tgtatctaca
     1441 aatccaatgg caatatttcc atgagatgta attataaact ccatccactc atcctctgtt
     1501 cttaataaat aaaatccttt aaaaattctt ttgaatctct aggagtttgt atcactgtca
     1561 cttcaactcc atcactaatt agttctttaa aagcctctct ctcttcttca gtaatattta
     1621 tcgtcggacg gatgcgttct gttccattcc ttcttgataa atttcctacg tttacacttg
     1681 ttattggtaa accgttatca atcaaaaatt taacatcgat tggagaaact gcaacaataa
     1741 gaactttttg tccttcatat ttaccagaaa gaatattttt aactgctact tcccttgtaa
     1801 ttattgaagt tctaacatta ggtggtgcag cgaggcgaac tacagctttt tttacgtcat
     1861 cattggctac ttcattatta ataaccataa ttctgttagc actcaattga tttgtccatt
     1921 gtgttgctac ttgtccatga atcattcgat catcaatacg aacatgtata atacctttca
     1981 ttttttcatc catattcaac acctacttca aaatatttaa cgctcttaac gcaatagaaa
     2041 ctactaagat taatagaatt acataaactg gtttaacctt tcgtcctaac aaataataca
     2101 caattgctac tacacatgca ggtaataaac caggcataac ttgatcaaag atttcatttc
     2161 cggtcattgt taagtcgcct tgtttaaatt caaagccaac attcactttt acaactgtgg
     2221 caatgagtgc accgaccacc attaacccta aaattgatac tgcatcagta atagacttta
     2281 attttgtttc aatttgagtt agtaggctcg cacccgattc tttcccagca aggtataaag
     2341 gataagcagt aatccttaat aaaattgatg ccagtatcca caaaataatt ccaacaaaac
     2401 taccttccaa tgccatatta gctgcaattg ctccaaaaat agtccatgga ataactacaa
     2461 atacactatc tccaattccc gctaaaggac ccattagacc tgttttcaga gcacttactg
     2521 cttccaaaga cttatttttt tcttcttttt caattccaac attcatacca ataatgtaag
     2581 gcgctgtata cggcgtagtg ttaaaaaatt gtaggtgagc taaaacggat tgtttcattc
     2641 cttcaggatc atctttatat gtgtccttga taaaaggaag catagaatac gcataaccca
     2701 acccttccat tttttcataa tttaatgaag ttgcaccatt gagaatccac cggaaaatta
     2761 cttgtttgtc gatttttgtc agtttcttat tcatcttcgt caaaacctcc tgttgcagct
     2821 ggtgctactt gagtattatt tgatctattt ttatatacga ttagtgcgat tgcagcgcca
     2881 agtaacgaaa tccctaaaat tggtatttgg aaataaactg ccaaaacgaa accaactaac
     2941 aaataggcaa agtaatcttt tgtaggtaaa tatttaagta gcattcctat cccaacagca
     3001 ggtaatagtc cccctgctac ctttaaacca ccggtaaccc attcaggaat ataattgatt
     3061 gcacttccca ctaacgttgg gcctaatatc acagttaaca aaacaggaat tccagataca
     3121 aagcttgtat taattgatgc caaatattgc atccactcta atttttgata atcttctttt
     3181 tgtgcgaatt tttctgcctt ttgttgaaaa aaaatgtttg ttgaccactt tagaacatct
     3241 agttgtacaa ttaacattgc taccggtatt gctaaagtta ttcctataga agcatcctgg
     3301 ttagttgaga ttgctaaata agtaccgatc aatgctgctg tttgataatc cggtactgat
     3361 gcaccaccaa aactagtaat acctagtgtc attaattgca aagtcccacc gatataaagt
     3421 ccagtttgaa tatctcccat aataagtccg gtcaagaaac ctgcaacgac aggttgggag
     3481 gttccgattt tgggcccttc cttatcccag ttaatgaaaa atccatacaa cacaactaaa
     3541 aatgtttgcc aaaacgccat tttgttctct cctttcttct tatactttta tataagcaat
     3601 tactgtgcca actaacatta tccaataaag cgggaaaata cagtaaatat taattaaatg
     3661 agcgtctaaa tatactttgg acaccctttc atttttttag acgctagtat gctatttaga
     3721 aaatagtctg ctcaaaaatt gtatagatta actcatactc tcct
//
LOCUS       DAZKOB010000172         3664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000172 DAZKOB010000000
VERSION     DAZKOB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.178"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..277
                     /locus_tag="ACG8M6_002869"
     CDS             <1..277
                     /locus_tag="ACG8M6_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0458447.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="ACG8M6_002870"
     CDS             420..641
                     /locus_tag="ACG8M6_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458448.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="ACG8M6_002871"
     CDS             657..1499
                     /locus_tag="ACG8M6_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458449.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="ACG8M6_002872"
     CDS             1562..3364
                     /locus_tag="ACG8M6_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0458450.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3664)
                     /locus_tag="ACG8M6_002873"
     CDS             complement(3423..>3664)
                     /locus_tag="ACG8M6_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0458451.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1307 a          519 c          746 g         1092 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaa
//
LOCUS       DAZKOB010000173         3603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000173 DAZKOB010000000
VERSION     DAZKOB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.179"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG8M6_002874"
     CDS             <1..186
                     /locus_tag="ACG8M6_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0458452.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG8M6_002875"
     CDS             329..553
                     /locus_tag="ACG8M6_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458453.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACG8M6_002876"
     CDS             568..1437
                     /locus_tag="ACG8M6_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0458454.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACG8M6_002877"
     CDS             1418..2152
                     /locus_tag="ACG8M6_002877"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0458455.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACG8M6_002878"
     CDS             2185..3093
                     /locus_tag="ACG8M6_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0458456.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACG8M6_002879"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACG8M6_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          790 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 ttg
//
LOCUS       DAZKOB010000174         3531 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000174 DAZKOB010000000
VERSION     DAZKOB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.180"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(257..961)
                     /locus_tag="ACG8M6_002880"
     CDS             complement(257..961)
                     /locus_tag="ACG8M6_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458457.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1148..1393)
                     /locus_tag="ACG8M6_002881"
     CDS             complement(1148..1393)
                     /locus_tag="ACG8M6_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458458.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1410..2261)
                     /locus_tag="ACG8M6_002882"
     CDS             complement(1410..2261)
                     /locus_tag="ACG8M6_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0458459.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2427..3431
                     /locus_tag="ACG8M6_002883"
     CDS             2427..3431
                     /locus_tag="ACG8M6_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0458460.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1098 a          601 c          687 g         1145 t
ORIGIN      
        1 tatcagaaaa tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa
       61 tcaggcaact ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata
      121 ggtgatttgt tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt
      181 tacttgctgt agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt
      241 atgacaaata gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag
      301 gaatcaaagt caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat
      361 tttcagtagt catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa
      421 taactacttc atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg
      481 aaaaatcagc aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt
      541 ccgcttttgg tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg
      601 tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca
      661 cgatttcaat aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa
      721 ttctctttaa tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat
      781 ttgttttccc agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt
      841 gtaacggcaa accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc
      901 ttacaccatc caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca
      961 tatgttctct ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag
     1021 gagtaatgca aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc
     1081 cgttttttga aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc
     1141 attctttcta ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt
     1201 catgttcgtg ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga
     1261 tgatttgatc atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca
     1321 aaaagacaaa atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt
     1381 cgattggatc cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct
     1441 ttgccgacta caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa
     1501 tcaaataatt cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg
     1561 aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca
     1621 gcgatttgtt tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct
     1681 tcgaggatca tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt
     1741 gcaaggaaaa cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga
     1801 tggtggggta agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct
     1861 tcaaatgctc gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg
     1921 tttggccagt ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct
     1981 tcaaaagaag acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag
     2041 aatatctctt ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc
     2101 ttgtatcctt gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag
     2161 gaaacagcga catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg
     2221 taaccatttg atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta
     2281 ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc
     2341 ttgaaatttt tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt
     2401 aaactaagac agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata
     2461 ttattgacag agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag
     2521 aatatattcg tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa
     2581 ctggacaact agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt
     2641 ttgtggaagc aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag
     2701 aagcacctag tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat
     2761 ctttattgaa agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag
     2821 ggatcgacaa ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag
     2881 atgaagcatt cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg
     2941 agttttctgg caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac
     3001 cttctttaga agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat
     3061 ttgagacaga agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag
     3121 aagaaaaagg tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca
     3181 cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc
     3241 ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat
     3301 caaacgggat cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat
     3361 ttgtgaataa tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga
     3421 atcgtttata aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa
     3481 ttgtgtcaag agaaggatcg gaaaagacga atataacaaa aaaagagcgt g
//
LOCUS       DAZKOB010000175         3504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000175 DAZKOB010000000
VERSION     DAZKOB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.181"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(123..800)
                     /gene="nth"
                     /locus_tag="ACG8M6_002884"
     CDS             complement(123..800)
                     /gene="nth"
                     /locus_tag="ACG8M6_002884"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0458461.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(807..1505)
                     /locus_tag="ACG8M6_002885"
     CDS             complement(807..1505)
                     /locus_tag="ACG8M6_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0458462.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1773..2957
                     /locus_tag="ACG8M6_002886"
     CDS             1773..2957
                     /locus_tag="ACG8M6_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0458463.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2963..3406)
                     /locus_tag="ACG8M6_002887"
     CDS             complement(2963..3406)
                     /locus_tag="ACG8M6_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0458464.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          895 a          709 c          593 g         1307 t
ORIGIN      
        1 gttaccatcc tagctactga gtatgtacat ttactttttc atgcaaaaaa gataaacgaa
       61 acagcttttc tccataataa caagataatt catacattta tttgcaatta aagaaaccac
      121 gtctacaggc gtggtttctt ttttagcgcc ttttctttta gacgcatccg atttttccca
      181 tcttgacata ttgatagcaa aggacatacc tcgcacttgg gatttcttgc cgtgcaatga
      241 tatctaccaa aaaaaatcag tgtatgatgt gtctttaccc ataattcttg tggtactttc
      301 cgcatcagtg tttcttctac ttccatgacc gtagcatcta gtttgcagat cctcaggcgt
      361 tttgagacac gttccacgtg ggtatctact gcgattgctg gtatcccgaa tgcatccccc
      421 aatacgacat ttgctgtctt tctgcctaca ccaggaagag acatcaattc ttctcttgac
      481 gttggcactt gcccatcgaa acgttcaatc agctgctgtg cacacgcttt gatgtttttt
      541 gctttatttc gatacaaacc aatcgttttt attttaagaa tgatttcatc gattgaagct
      601 tctgctaacg catcgggtgt cggaaaagag gcaaacaaat ctggcgttgc tttattcact
      661 gatacatctg tcgcctgggc acttaagatc actgcaatca ataactcaaa tggattattg
      721 tgcttgagtt ctccatgtgc ttcgggaaac attccataca tcgtttctag agcttccatt
      781 gttttttgtt tgcttaacat attccgctac tcctccaacc aatttttcag tgagactttc
      841 ggcaattctt cttgttgagc tgactgttct acttctcttt gaagaagctg ctgttttctt
      901 cgtttttggt cttcttccac ttgttgtttt gtacgtagat tttttctttc ccacgatagc
      961 aaaatgcggt caacgtaatt aaaactataa gcctgattta atacggcttc ccgcagtgct
     1021 agcttcaaaa tttccggctg ataatggtct tcttctagcc attgtccgat ccgctggaac
     1081 tcgatcgaag aaagcgggcg tccaaattct tgttcaaata attgatacac tgactggatt
     1141 tctttttctt gtgttttttc ttcgtatttt ttttcttgtg tttttaaata ctcacctaat
     1201 agatcataga tggggtaaag attatatcga tctgcttttt taccgttgac aatcgtcgtt
     1261 tctatcttga taaatccatt tgttacgaga cgatccagta tttgaaaaat ctgatcttgt
     1321 ttgattccca tatccatagc aatcatctgc aaatcaggga aagtatctcc ttcaagctgt
     1381 gccatatgca actgtagaac aaataaaaat tcttcagatt gtagaccgat cctatgataa
     1441 tttttaagta aaagattaga tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc
     1501 aacatgattt ctcctccgct caatcacagt atacgtaaaa aaaacatggg aagcaagaac
     1561 aggtttcttt tctatttact tcactgatcg gtcagattta caatgtcgtc tacttgattt
     1621 ttgtttgttc atgtttttga tgcgtatctt cacttttcgg aataaataaa ttaagtagat
     1681 ttcgctgttc cttagaatgt tgacaaggaa acaaaattac tatacactat ttttgtttcc
     1741 aagttaacat tattgcataa aggagttttt acatgtacag tttcacaaat aaacattcta
     1801 aagtttttgg atacgtctct gtcttattca ctggattagg atttactatc gtcagtcctg
     1861 tacttccgtt tttaacgctg ccttattctc actccataca tcagcaagct ttctatatca
     1921 ctttgctcat gtctgtttat gctctagctg ccttcttatc tgcccccatt ctcggtagct
     1981 taagtgatca ttttgggcgt catccgatcc ttattatcag tttattagga tcaagtatcg
     2041 gatatttgct ttttggttta ggaaattcga tatggatgtt gttccttggt cgtatcattg
     2101 aaggattgac ggcaggagaa atctccacct tatatgctta ctttgccgac atcacggaac
     2161 ctaatgaaag aaccaaagtt tttggatgga tgggtgcttt agtcggtata ggaacaacgc
     2221 tgggtcctat tatagggggt ctattagctg agttggggaa ttctgttcct atatttattg
     2281 gagcactctt tactttctta aatgccgttt atggctacac atttatgcta gaaagccttc
     2341 caataaaaaa acgttcagta gatttaagtt tctcccatgt aagacctttt caccaattac
     2401 aacaattatt caagatttca tccgttattc ctttattgac agcaggtttt gctgtttggt
     2461 tggcagctgg ctctcttcaa tctatctttt ctcaattttc gattgacgcc tttcagtgga
     2521 aggctgggtt ggttggttta agcttctctt tgattggaat cttggatact gtctcgcaat
     2581 tgttcattat gcctcgattg ctcaaaaaat tttcagaaca gcagatcaca agaataggga
     2641 tgttcagtga aatcctcgcc tatctattca tcacattatc tggaattctt ctactgccga
     2701 ttttgttttt atttgggatt atctgttatg gtttcggtga ttctattttt actcctgtat
     2761 tcaatggcca actatccaat tctgtctcag ataaccagca aggtcttgta atgggcggta
     2821 cccaaagtat acaatctcta tcgcgcgtca tcggcccgct aatcgcagga cagctttatg
     2881 ctgcagcacc atttcttccg acggtattcg gatttttgct tatgattttt gcctattatc
     2941 actacaataa aaaatagtag cttcatttgc tacttcgtat aaattctctg tatcgaccga
     3001 taaaacgaaa tactgtgtca aattcttctt ttgacatatt ttcaaataca gtagcatctc
     3061 gctgtaccca tttttgatgg agttgctcgt gtctattcct tattttttgt ccgattggcg
     3121 tcaattgaaa atagatttct tttttattct cttcgctttg ataagtgttt atgatccctc
     3181 tagctatcag tttttttgtt agcttactga tggctcctct agtcatgtaa ctagcattcg
     3241 ctaattttgt cacgttagga tcagggattt tcccgatatg ttcaatcact tctagttcat
     3301 ttagagtaaa tcctgcaaat tctttttcca tttctggttt attacgatga ataatctcat
     3361 tagctaaatc cagatattct tctattagca gttgtctttt atccatttct acaccttctt
     3421 tttctttcat tatattgttt ttttgtttcc tattcaacaa agataaaaat attttgtagg
     3481 aaaacccaat ctgaaaccaa gaaa
//
LOCUS       DAZKOB010000176         3474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000176 DAZKOB010000000
VERSION     DAZKOB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.182"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..409
                     /locus_tag="ACG8M6_002888"
     CDS             92..409
                     /locus_tag="ACG8M6_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0458465.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            406..567
                     /locus_tag="ACG8M6_002889"
     CDS             406..567
                     /locus_tag="ACG8M6_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0458466.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            570..869
                     /locus_tag="ACG8M6_002890"
     CDS             570..869
                     /locus_tag="ACG8M6_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458467.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            869..1183
                     /locus_tag="ACG8M6_002891"
     CDS             869..1183
                     /locus_tag="ACG8M6_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0458468.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            1176..1706
                     /locus_tag="ACG8M6_002892"
     CDS             1176..1706
                     /locus_tag="ACG8M6_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0458469.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            1703..1972
                     /locus_tag="ACG8M6_002893"
     CDS             1703..1972
                     /locus_tag="ACG8M6_002893"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458470.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            1902..2105
                     /locus_tag="ACG8M6_002894"
     CDS             1902..2105
                     /locus_tag="ACG8M6_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0458471.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            2102..2389
                     /locus_tag="ACG8M6_002895"
     CDS             2102..2389
                     /locus_tag="ACG8M6_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458472.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(2422..2673)
                     /locus_tag="ACG8M6_002896"
     CDS             complement(2422..2673)
                     /locus_tag="ACG8M6_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458473.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            2876..3289
                     /locus_tag="ACG8M6_002897"
     CDS             2876..3289
                     /locus_tag="ACG8M6_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0458474.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT         1283 a          517 c          783 g          891 t
ORIGIN      
        1 gaataggttt ttgggtctga ccctccaaac tctccaccaa aatcttccta aatgctttga
       61 taccaacgat taaacagtct ggagggttac attgaaatca gaacatcaaa tacaagacga
      121 gatcagaatt gccttatcaa aacatggttg cacgattttt cgatccaatg cgggacaagt
      181 gacaacgatc gaagggcggc aattttacgg gatgccaaaa gggttcccag atctatgtgg
      241 acatcgtaat tctgatggga aagcaattta tatagaagta aaaaatgaac gcggtaagtt
      301 gcgtgaagat caaaaaaggt ttgctgaatt tttaaacggt caacctgtac tatacggtgt
      361 tgcacgatca gtggaagaag cattgaaaat tgtggaggaa tcatcatgac aacagaagaa
      421 gtgattcaaa tgcgtattcg aagccttcag cgtgagattg acgatctgga acggacaaag
      481 gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt tgcacataga gaatttaaga
      541 agggaaatcc atcgattgga ggaatgagcg tggataagaa agcagcaatg aaacgaatca
      601 tcgaactgac acattctgag aattggcaag aagacaaaga aatagttgca gaagtccaaa
      661 agctcggtaa atcaatgtgg agtgaaaaaa ccaaacggag aacaccgaga aaaattgcaa
      721 tctggcatgg tgaccgaatt ctagtaacag gtactgctga acagttatct gaaattactg
      781 gattaagcaa aaacattatc tgggatagag ctaaacgcga gaacgttgat tcgaaaggtc
      841 gtcaatttaa acattgggag aagaaataat ggaggaacta atcacaaaag tagagcagtg
      901 ggcaaaagat aagggattgg atcaagctga ttccagcaag caaatgttaa aaacgatcga
      961 agagattggg gaagttgccg cttctctagc tagaaaagat gaatatggtt taagagacgg
     1021 aattggagac gtagtagtaa ccttgattat tttagctatg caaaatgata tggatttgta
     1081 cgagtgtctg aaccaagcat acaacgaaat caaaggacgc acaggaaaaa tggtagatgg
     1141 tgtattcgtg aagtcgagtg atttggagga cagcgatgaa taaaaaagta ttaattgata
     1201 aacaagcatt gattgatgaa ttaatgaaaa tacctggtgt gggatctaat agtgacgctt
     1261 tagaaacgat taaacgtttc ccatcgtatg aaccgcagaa gccagttgtg cctaagtttg
     1321 tggcggaact actcgactat tatcgtcagt caacagatgt tgatttatta gctttattga
     1381 taactttcca tgattggtat tatcgcaaaa ctaaagacgg tgagcatgaa gaagcaattg
     1441 attggctagt tgatcatcct gaaatttata tgcgtgcatg gctgtttggc tacgagctcg
     1501 agaaagagcc gttgtattgc gtaataatca atggtagata tcttgtcaaa gtattcagca
     1561 atacaaacgt tgtcatatta gttccagcag atgaattttc acagtacgtt actcaaaaat
     1621 ttcaattaac tgaaaaacaa atcaaagcaa ttgatgaaag atactggccg tttgctgtgc
     1681 cagtggatga ggtggtagaa ggatgagcga cccatacatt actaaattag aaaagttgtc
     1741 cgaagatttt gagaagaata aaaataaact aactaaagat aaacttatag aactggttag
     1801 gttggttatc agaggagcta ttacaagaga aaaggcgcag caaaacatag aaatttttgc
     1861 tcgtagtgtc cgagatggat tagagaagaa agttaaacaa aatgaacaac agacaccgca
     1921 gaataacaaa actaagaaaa cgggaactga atgtactaaa gacaaagttt gaaaaagaat
     1981 atggaatttc agcagaagaa acatataaag tggcaagtca gtgtgttgct gatgcgagcg
     2041 gcgctattcg taagtttgga atttcgatat taagtgatga tcgtaaatgg gaggaaaaag
     2101 aatgaaacta aaagacggat tttactccag cagtcatggt atcggcggtt taatgctaga
     2161 tatgccgaca aagaacccta aaacacgtga gaaaccaaaa ttcaaagtcg gtgacatggt
     2221 tcgctgtgaa gcagaaggat tcatctatcc attttgtgga tatgtagaac atctctataa
     2281 tcactcagca atcattcgta ttgaaaacac gatggaatgt gacaagtggt tagcgaaaag
     2341 caaagagaat ttagcagtag cgagattggt ggatatggag gttatataaa cagcgaaaaa
     2401 atgtacttct aagagaaaaa tttatttttt ataaaaatag gaaagtatca ttatgaaaaa
     2461 gaccacagag ataaataaac ctaccacata aaaaatgaat atttcttgcg attcaatata
     2521 ggtatattta acaatttgta caataaaaaa tactataggt aaaaaagata acatgaggag
     2581 cttatttgtt ctagagtgtt tgacaaagta acaaaatgcg acgataccaa tacaaacggg
     2641 aatccaaaat aacagcatgc tccacatagt cactcaacca tctcctatac gctttattat
     2701 caatagataa tatcattttt cgtaataaag ataaaaaaat aagtaaggct tctcattaaa
     2761 atataaaaaa gacagccgac cactgactgc ctatataaga gtattgaaat aaaaagctgc
     2821 tgatataata aattccacaa gtttattata tcacataaag gagcggtttg acttgatgca
     2881 attgttacga gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa
     2941 ctttcggcgt ttggagcgga tggcaggtcg ctctttgata gatattaagt cgccgattat
     3001 tacggatatg ccgaaggcgc cgaagcacgg caataaggca gaagacgcaa tcattcagat
     3061 gatggatata gaagcggaga gagacgcgat tctagcagcc ttgatggctc ttagtctgat
     3121 tagtcgtcag atactctact atagcttttg tgtgccagat agcttctcaa actacagaat
     3181 tagccgtgaa gtgggttatt cagaaagaag tatacaacgg atgaagtcgg aagctctaat
     3241 agagtttgca gaagcatata aacacggaag aataattgct tataaataat ttggcggctt
     3301 tttggcggaa tgatggcggt ttttagctat ttaccagtga tattatggta gtgtcgaaag
     3361 attagtgata ggtctaagac aaaataataa taaaaggaac atcgttttat tattgtttca
     3421 caattaagct tcgatagaca gcaacggaaa tattaagaat aaggatgtga attt
//
LOCUS       DAZKOB010000177         3445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000177 DAZKOB010000000
VERSION     DAZKOB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.183"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8M6_002898"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8M6_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0458475.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8M6_002899"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8M6_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          978 a          759 c          539 g         1169 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ataacaaact aacta
//
LOCUS       DAZKOB010000178         3386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000178 DAZKOB010000000
VERSION     DAZKOB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.184"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG8M6_002900"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG8M6_002900"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0458476.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACG8M6_002901"
     CDS             complement(2261..2512)
                     /locus_tag="ACG8M6_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0458477.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACG8M6_002902"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACG8M6_002902"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0458478.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          922 a          745 c          626 g         1093 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccct
//
LOCUS       DAZKOB010000179         3200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000179 DAZKOB010000000
VERSION     DAZKOB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.187"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8M6_002903"
     CDS             301..585
                     /locus_tag="ACG8M6_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458479.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8M6_002904"
     CDS             588..1004
                     /locus_tag="ACG8M6_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458480.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8M6_002905"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8M6_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0458481.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2522..2608
                     /locus_tag="ACG8M6_002906"
     CDS             2522..2608
                     /locus_tag="ACG8M6_002906"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0458482.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..>3200)
                     /locus_tag="ACG8M6_002907"
     CDS             complement(2911..>3200)
                     /locus_tag="ACG8M6_002907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458483.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt attttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DAZKOB010000180         3114 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000180 DAZKOB010000000
VERSION     DAZKOB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.188"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1191)
                     /gene="dnaN"
                     /locus_tag="ACG8M6_002908"
     CDS             complement(61..1191)
                     /gene="dnaN"
                     /locus_tag="ACG8M6_002908"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0458484.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            complement(1389..2723)
                     /gene="dnaA"
                     /locus_tag="ACG8M6_002909"
     CDS             complement(1389..2723)
                     /gene="dnaA"
                     /locus_tag="ACG8M6_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0458485.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            complement(2872..>3114)
                     /locus_tag="ACG8M6_002910"
     CDS             complement(2872..>3114)
                     /locus_tag="ACG8M6_002910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458486.1"
                     /translation="FLWESYVEKVMHRDYPQIIKGWFYTENTRVKKVIHNQAISCVFR
                     LLTIGISVFFIHRRKRKSIGFYEFSTCALVVWKTFF"
BASE COUNT          939 a          589 c          562 g         1024 t
ORIGIN      
        1 taaaatttta ttttttctat ttcttttcaa ttaaattttc aagaacattg aaaatcattg
       61 ttaatttgta cgtacaggag taatcagctg aataaattga acgccatctt ccgttggttc
      121 caatgtaaat ggacgaatag cagagataaa tttcactttg atgctcatat ctccaaatgc
      181 acgcaacgct gctttcatat aatccggatt gaaagaaata tctaatggat cgccactgct
      241 tgctgtataa ctcaagcttt cttctacttt cccgatttct ggcgaatttc cgtataaaac
      301 aaccgcatct ggacgaatag ataaacgaac gatgttgtta cggccttcat gagaaagtaa
      361 agaagcccgt tcgatcgccg ctaaaaagct aggaacagaa aattcaacct ctgtattaaa
      421 acttgaagga atcaaacgat tggtatctgg atagtttcct tctagcaaac gggaatagaa
      481 atacatcgtt tctgttttga acaacacttg gttttccata atgctgattt cgacgatttc
      541 ttcttcattt gttaatgagc gagataattc gatcaaactt tttccaggaa taacaatatc
      601 aaaatgatca gccgcttgtt ctactggaat cacgcgttga cttagacggt gagaatcagt
      661 agctacagct aataaagaat tatcagataa aatgaaatgc acacctgtca agattggacg
      721 actctcatgt tgtgagacag caaaaactgt ttcgttgatt agtttagtca atacgtgtac
      781 aggtaatttc atctggttat ggctttcgac aacaggaaga tgaggatagt tatctgcatc
      841 taatccattt acgataaaat tcgcttttcc agaagtgatc gctacttgtt tattttctaa
      901 aacttctaaa gtaaatgttt cttcaggaag tctccgaatg atttcgctaa agaaacgtgc
      961 ttgtaaaaca atggaaccag tagattcgat ttgcatattt gctttttcgt tttcaacaga
     1021 caaaaaagtt tcaattgata tatcagcgtt gctccccgtc aaagtcaaac cttcttgtgt
     1081 cagtgtgatt tttacacctg tcaaaatagg gatcgtggtt ttgcttgaaa tagctcgttg
     1141 aacagtttgc aattcctgca taaagctagc tcggtttaaa gtaactttca tgatgtatct
     1201 cctttgaatg tttgtttatt tattattagt taaaaagaat aataaaagta atagggcatg
     1261 ttgattctgt ggaaaactca aaaaacgtaa gagagaaacg aaaaaacagc tgtggaaaaa
     1321 gtgtgtataa cttttgaacg tttttttctt ttatccacat gctattttca gttgatagaa
     1381 tcgacaaatt aatttagtaa aaagtttttg atttctgata cttcattttg catactgaca
     1441 ctagaatcca tcagctgctg gattttttca tgagcatgga taaccgttgt atgatccttg
     1501 ccaccgaatt cagctccgat tttcggtaaa gaattatctg tgagctcacg tgccaaatac
     1561 atagcgattt gtctaggaac aacaatagat ttgacgcgtt tctttccttt gagatctttc
     1621 aattggatgt gataatactt tgccacttct tcttggatct gcaggattga caattgagga
     1681 tgtccttttc ctgattttaa cgttttcagt gcgtctgcag ctagactagt cgaaatatct
     1741 tcactattca tcgcagcaaa agcttgcact cgaacaagcg caccttctaa ttcacgaatg
     1801 ttagaatcaa tttgtcctgc gatgtagctt agtgtgtcat ctgggatctc tagacgttca
     1861 gccgccgctt ttttacgtaa aatcgctgtt cttgtttcaa gatccggcgg tgtaatatca
     1921 acagacagcc cccatgcaaa gcgagaaaca aggcgttctt gcagtttcgg aatctcgttt
     1981 ggcaaccgat cactggttag tacgatttgt ttcccttcat tgtacaaggc attgaaagta
     2041 tggaaaaact cttcttgcgt agcttctttt tctgcaaaaa attgaatgtc atccactagc
     2101 agtaaatcaa catttcgata ttcttgacgg aactcttctg ctgttttgtt ttggatcgaa
     2161 ttgatgaaat cattagcaaa agtttcactg cttacatatt ttactttggc attaggctga
     2221 ctttgcagca tttgatgacc aattgcatgc atcaagtgag tcttaccaag tcccacgcct
     2281 ccataaaaga agagcgggtt ataaatagaa cctggatctt ctgctacaac gagcgctgct
     2341 gcatgagcca tctggtttcc tttaccaatg acaaacgtat caaaggtata tttagggttc
     2401 agcatggctt tttttagcgg actaggtgcc acaacttttg gatttttttc ttctataact
     2461 tcttcttgct ctgcttcttc gccagtaata aagcgaggga taatctcatt tcctgataac
     2521 atataaccga cttcaacgat ttttgtcgcc aggttttttt cccaatattc tttatgaatc
     2581 ttgctaggaa cctcaacaac gagctggttt tgatcaaggg ttcgagggtt cgctgtttcg
     2641 atccatgtat tatagctagc aggcgataaa tccttttggt atgttgcttt taattcattc
     2701 cataaagcat cgagggatac catataaggc ctcctccttc attaaaaagt gaataactag
     2761 tactaattta gcattttttt caatagtttt ccacagaaac cgaagaaaaa aattttttaa
     2821 ttacaattat ccacacaatc gacaaaaaga aaaaagagaa aaacacagtt gtcaaaaaaa
     2881 agttttccac accactaatg cacaggtgga aaactcgtaa aaaccaatag atttcctctt
     2941 gcgtctgtgg ataaaaaata cagatatacc aatagttagg agacggaata cacaggaaat
     3001 cgcctgattg tggattactt tttttacacg tgtgttttct gtgtaaaacc agccttttat
     3061 tatctgtgga taatccctgt gcataacttt ttccacatag gattcccata gaaa
//
LOCUS       DAZKOB010000181         3099 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000181 DAZKOB010000000
VERSION     DAZKOB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.189"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(319..933)
                     /locus_tag="ACG8M6_002911"
     CDS             complement(319..933)
                     /locus_tag="ACG8M6_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0458487.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1539..1916)
                     /locus_tag="ACG8M6_002912"
     CDS             complement(1539..1916)
                     /locus_tag="ACG8M6_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458488.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2216..3097)
                     /gene="galU"
                     /locus_tag="ACG8M6_002913"
     CDS             complement(2216..3097)
                     /gene="galU"
                     /locus_tag="ACG8M6_002913"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0458489.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          939 a          648 c          478 g         1034 t
ORIGIN      
        1 gaagactctt tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt
       61 gtctcttttt ccactacttt ttatttttct gacactacat attcttataa ctcatttctc
      121 ataactgtga aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa
      181 gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata
      241 atcgttttct ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact
      301 ttgtcaacat gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa
      361 atacaaaact tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg
      421 atctagattt ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa
      481 atcagcaaat tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa
      541 atcaaatcca gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc
      601 gatgattgcc gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac
      661 agaaagctga tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc
      721 tggattttca atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc
      781 tacaccactt agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt
      841 cgcgatccaa gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa
      901 ttggtgataa tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga
      961 catgaataga aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt
     1021 aacacatgtg gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata
     1081 ccttgacata gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa
     1141 ttttcaccct agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa
     1201 tgatcggtca gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg
     1261 gtcagggagc ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta
     1321 tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa
     1381 cataaaaaaa ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt
     1441 atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat
     1501 ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt
     1561 ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct
     1621 atccgagact ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat
     1681 aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc
     1741 ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga
     1801 aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc
     1861 atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg
     1921 aacacacacc tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg
     1981 gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa
     2041 accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc
     2101 cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa
     2161 gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac
     2221 tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt
     2281 ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt
     2341 cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa
     2401 gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa
     2461 atattttagg agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca
     2521 tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag
     2581 gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg
     2641 aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca
     2701 tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa
     2761 gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag
     2821 tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca
     2881 attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca
     2941 agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat
     3001 ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta
     3061 gcccagcggc aggaatgact gcttttttga ccttcactt
//
LOCUS       DAZKOB010000182         3071 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000182 DAZKOB010000000
VERSION     DAZKOB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.190"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..1102
                     /locus_tag="ACG8M6_002914"
     CDS             287..1102
                     /locus_tag="ACG8M6_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458490.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1228..1485
                     /locus_tag="ACG8M6_002915"
     CDS             1228..1485
                     /locus_tag="ACG8M6_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458491.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1737..2309)
                     /locus_tag="ACG8M6_002916"
     CDS             complement(1737..2309)
                     /locus_tag="ACG8M6_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0458492.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2325..2930)
                     /gene="fic"
                     /locus_tag="ACG8M6_002917"
     CDS             complement(2325..2930)
                     /gene="fic"
                     /locus_tag="ACG8M6_002917"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0458493.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1018 a          539 c          473 g         1041 t
ORIGIN      
        1 aaataagaaa ttcaaaaaag aaaaaacttt ccttaaaaac aaaaaatagg agtattcagt
       61 ttcttaatag ttttgtaata gttgtatgta gaagttttct taatagtttt tgtaataatt
      121 tattaatcgt agtttatata acttatttgt aagtgactag agaattaaat aaaaaataat
      181 tactatctag tacgaaatta attacatagg ataatatatt aaggtgctgg actacaagta
      241 aaaaatactg tatgatcatg acagattcaa gaaaggagca ataagattgg ttaagatagc
      301 aggactttct aaaggtggaa aaaccgtttc acaactttat aaacaaaact acagagactg
      361 gagacagttc agaaaaaaca ttaaggattc ttactttatt ctaccaacag aacttgaaaa
      421 atatttaccg aataacaaag ctattaattt atatctgtat tactgtttta aggcaaagaa
      481 tgaaggcggg gattcctggc attcggtcaa taccatagca gagtctctag gagtgacaac
      541 taaaactata aacacctgga ataaaacctt agttgattta ggtatgattg cacgtataga
      601 cgataaccat ctcagtaaat cgacaatttt acttcctctc tctagttact ataaattagc
      661 ccttgatagt tctttaaagg atgttaccga gagcactgtc catgaaattg atggcaactt
      721 ggttgcagtt tatcatttgt ttgaatggag aaaaaatgag gctgatgaaa attataacgt
      781 tccttataac acactctgtt gtgtttttaa aagaaaaaca caaaaagata ctatctataa
      841 atttatcttg gtccaagatg agaaaataag taatttcgaa ttaagcactc cttctagtaa
      901 gatttatgac gatgcatata gatttgaatg tcaagatcta gaagccctcc tttccgcaaa
      961 taatctaaac aatatagaac atgaaaactt tgttgtaaac actagattta atttagtttc
     1021 tgaaaaggac tcagatttgt tagatttgct gatagagctt actaaaaatc tagataagaa
     1081 agaaaacatt actgtactat aactagcatc ggtccatcat taagtttgga ctattagaac
     1141 acttgctatt acagaaaaag gtatgctagc cacgcttact atttattggt atcaaacaag
     1201 tatcttaatt tgaaaggaag atgatttatg gctaatttat atatagagaa caaaggagac
     1261 ttgaatgcca ttattacttt aatcgtagtt atggtaaaat caataggtaa tatcacaata
     1321 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
     1381 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
     1441 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
     1501 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
     1561 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
     1621 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
     1681 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1741 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1801 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1861 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1921 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1981 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     2041 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     2101 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     2161 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     2221 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     2281 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     2341 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     2401 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     2461 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     2521 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     2581 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     2641 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     2701 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2761 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2821 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2881 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2941 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     3001 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     3061 gcacagtcta g
//
LOCUS       DAZKOB010000183         2977 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000183 DAZKOB010000000
VERSION     DAZKOB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.191"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..703
                     /locus_tag="ACG8M6_002918"
     CDS             <1..703
                     /locus_tag="ACG8M6_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0458494.1"
                     /translation="GWTPDVIIGRKERPVSCGMRTLYRLFSKGIFDIDTLPMKGKRKP
                     NGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVI
                     ITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFAD
                     PGTPSQRPLNENSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRTPIE
                     IFLSYVQEAFYSSLI"
     gene            complement(1569..1994)
                     /locus_tag="ACG8M6_002919"
     CDS             complement(1569..1994)
                     /locus_tag="ACG8M6_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0458495.1"
                     /translation="MHRKVRITRKSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISI
                     EGILRIQGPAFIAQGAHIDIRKNAVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDT
                     LFMDTDSHTIFELNTHRILNKNNKIVIGKHCWIGAKSVI"
     gene            complement(2096..2713)
                     /locus_tag="ACG8M6_002920"
     CDS             complement(2096..2713)
                     /locus_tag="ACG8M6_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0458496.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          979 a          571 c          369 g         1058 t
ORIGIN      
        1 tggttggacg cctgacgtca ttatcgggcg aaaagaaagg cctgtttcat gcggtatgag
       61 aacactttat cgtttatttt ctaaaggaat atttgatatt gacacactac cgatgaaagg
      121 taaaagaaaa cccaatggcc atcaggaaaa acggggaaaa caacagtatc agcgctcaat
      181 ccatgatcga cctgataatt atcctgattt caattctgag tttggtcacc ttgaaggtga
      241 tacgatcgtt ggcattcatc ataaaagtgc cgtcattact ttagttgaaa gattatctaa
      301 agtcattatc acgattaaac ccaacggccg taaggcatta gatattgaaa ctgcccttaa
      361 tcaatggttt tctcgcttcc ctaaaaactt ctttaaatct attacgtttg actgtggaaa
      421 agaattttct aactggaaag ccattagtaa ccaacatgat attgatatat attttgcgga
      481 ccctggaaca ccttctcaac gcccattaaa cgaaaattct aacgggattc tgcgtcgtaa
      541 tggactgccg aaatcaatag attttagaga agtgaatcag acatttattt ccagtgtcag
      601 caatcaacgc aatcatattc caagaaaatc attgaattac agaacaccaa tcgaaatatt
      661 tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca tataatttaa
      721 aattaacatt gattctccta tgaaaatatc ttttaccttt ctcctgtgaa aaggaaattt
      781 ccttttttat agtgttattg atataatcag aatattcatc aaactctgta tctggagtta
      841 aaaatctaca gtaacatcta taagctttgc tagtaatctt cttgggttct ctctccattg
      901 tcaagcaaca tcaaaacacc tacttactat tcgattcttt tctgccaaaa acgagtcagc
      961 atcactcaca acttatgtgt tgctgactcc gactagtcct ttgttcaata tcaacttatt
     1021 aaacagatat aatatttttc tttatttcgt atactccaag acttccatct ttctttcatc
     1081 ttatctctga aaaaaacaca gcaaagtgcc cttaataaat atacattaga atttgcaaat
     1141 tttttcatct taagatatat cctattcata ttctttacaa tcctaatggg tgattacaaa
     1201 tctatttagt tcaagttcta cattataaac acctttcttg atagtatcat ctgctgcaca
     1261 agcgatgtcg atccacttat taggggattt acgtgctcaa aagtggctat tccttttggg
     1321 aagtccatcc ataataatgg tccatggatt tttttagggt cttgtcgttc aaatttctca
     1381 aaaactttat tatacttcca tagttttata attatttggt ttcactttgg ctcgttcaaa
     1441 caccaacttg tatttctttt caacgttcga actggagccc cccctataat ctcattatta
     1501 ttttcatgac tttttactac cgtggaatta caagcaacaa caatattatc tgctaaatga
     1561 acacctttct atatcacgct ttttgctcca atccaacaat gtttacctat aactatttta
     1621 ttatttttat tcaaaatcct atgagtattc aattcaaaaa tcgtatgtga atcggtatcc
     1681 ataaataatg tatcccacga tactgtacag ccatctttga attgaatttc cttaaagcaa
     1741 attatactca tttttcctgt cgaattaaac tttgacccaa tatctaaaac tgcatttttc
     1801 cttatgtcaa tatgagcccc ttgtgcgata aacgctggcc cttgtattct tagtattcct
     1861 tcaatactta taatcgtttc ttcatttccc gcagtattta atactggaaa gcccaaataa
     1921 actatttttc cagattgtaa tactatctca attttccctc tcgatttgcg tgtaatccgt
     1981 actttacgat gcaccaaaat aggtaatctc cacgcatctg aaatatgaag aaatttataa
     2041 ttgaagtata tcgttttagg taaatttaat atattcttta ttatcaatcc gagtctcatc
     2101 tttcaccatc tctttttgta atcaaaatta gtttataaag gattacaccc aatataccgc
     2161 ccaacgtatt atatacaaaa tccgaaattt gaaacgttcc tagcctcaaa aaaagttgta
     2221 aaaattcaat tattgctgaa aaataaaatg caatttttcc tccatatact attgaggacc
     2281 atattttggg tttctttata ttttcatatt taatagacat taccatataa acatatggaa
     2341 taaaaagaat aatattttct aaacacgctg tagataactc ttttgttcca gttgttgtat
     2401 tatacttata aatccaccag tctcccataa catcagataa aggatttaac caaattgttc
     2461 gattgattaa tgttgtaaat aaaatcatcg cagtatagaa tatcaaagca caaacttttc
     2521 tatattttct atccttcttt aaatgaagat aaagttccat tagtatacgt ttatagccct
     2581 cacctgcagc aatgccatct gttgcataca taaaatagac cagtcctaaa attgtcaata
     2641 aaacagcaaa cccaaagggt tcatataatt tatataatat atctgtaata atttctgcaa
     2701 tcactattat cataattatt tctccaatat caagtataca cttatcgctt ccaattacac
     2761 atgatcatat tagagtgtat tatcacattc tctaatattc aatttatttt tcctaaaata
     2821 ttcgaaataa tcgtcttgat aacgagtaag tatttttata cgattgataa catagtaaca
     2881 atatttttaa ttatagtaaa aaatcatctg cctttttcat ccacctaatc ttatttttca
     2941 ttttattcaa catccttaaa taaacttccg ttcctaa
//
LOCUS       DAZKOB010000184         2941 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000184 DAZKOB010000000
VERSION     DAZKOB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.192"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG8M6_002921"
     CDS             14..1141
                     /locus_tag="ACG8M6_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0458497.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACG8M6_002922"
     CDS             complement(1359..2501)
                     /locus_tag="ACG8M6_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0458498.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ACG8M6_002923"
     CDS             complement(2506..2691)
                     /locus_tag="ACG8M6_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0458499.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          973 a          537 c          462 g          969 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 t
//
LOCUS       DAZKOB010000185         2935 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000185 DAZKOB010000000
VERSION     DAZKOB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.193"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..146)
                     /locus_tag="ACG8M6_002924"
     CDS             complement(<1..146)
                     /locus_tag="ACG8M6_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFQ0458500.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYE"
     gene            complement(276..1214)
                     /locus_tag="ACG8M6_002925"
     CDS             complement(276..1214)
                     /locus_tag="ACG8M6_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0458501.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1705..2922
                     /locus_tag="ACG8M6_002926"
                     /pseudo
     CDS             1705..2922
                     /locus_tag="ACG8M6_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         1033 a          490 c          456 g          956 t
ORIGIN      
        1 atttcatatc ctttcttttt tatcatatct ttaaacaaag taatatcaat ttctttagta
       61 atgctataag tagtttgctg attaaaatag tgttcaaaat attcttttct atcccaattt
      121 tctaattcaa taatattaaa agtcatatat aacttcctcc taaattttaa atttttatat
      181 ttaggaggaa taatcctctg attttttcat acgttatgtc acctcgtaaa tattaattat
      241 actgaattag caatttttat caaataaaac ttattttact tccaaaacct aaattcacgt
      301 tgccaaaaat caatctgctt ttgcaattgt ttttcgttcg cttttaaagt cgatttcatt
      361 aattccgtta aatcaattgg agatatttct ctaatcaatt ttttaaattt agtcttagta
      421 ttcttactta gctttcccca catactttct tcatgcaaca aagtataaac catagcttgc
      481 tcattaattt tttctaaagt agcccacgca ggtttcaaga tgtgtaaatc attaaaacaa
      541 tcattccagt aatcaaccat atctcttttt aattcaactt ctacacgcca taaatgttca
      601 gacacaactt caacatctgc gttatcttta cgttcttgtt ttttattata aattctaata
      661 aatctatcac tatcacggac accaaaatat tttgtttctg gcttgccatt acgaccataa
      721 aaaacagttt tcttaactgc tttatcagtc attgcatagt aatcgctcaa atcatcttca
      781 aaatcaaaag ctaagtctaa tcttgtaaaa ccgtcatctt ccatgtagtc gataatattt
      841 tgttttaacc aaatcatttc ttcatgtgtg agtttattgg gattaaattc aacacgcata
      901 ttacgtctat cccaagtatc tgcttttact ttgtcatatt cgatataaac tttttcttga
      961 agtgccttag ctttaaactt tgtttgaagt atatcccaaa gtcgtatttg tggctctaca
     1021 ctcataaagt cagatagctt tttagcatta gttttgttca aatttccaac gattgtcatg
     1081 gcatcaaaac ttaatgcggg ttgagatttt cccaaagttt gaccacttaa ccggctatta
     1141 cttaaccggc tattagagac ggccggtttt tttagtgaga tttctgcagc taatttttca
     1201 tcaattttct tcatttttta tcgctcctaa aataattttt ggaacgaaaa aaggacgagt
     1261 ggaatcgcaa aataaatttc gcctgtatcc attcgtccat tttgttttgt attcaaaatc
     1321 aaaaattgat atactaatca tcgaatgaat aacaagtgtg gtttgttatt ccgggcgaga
     1381 acgttggtag cgttgctcgt ctttttttat gtcttttttt cgttaaaact attttaaaac
     1441 taaatatatc ttaaagcaaa taaattcaag gggctaaaag cgccccttaa aaaccccttg
     1501 cctctgaaaa gttttgtgtt tcctttccgt cggtttcaaa agctaattta ctcaaaaaaa
     1561 tagatcaagg gtaaatgcac tcactgcgtt cgcccttgag ctattttatt tcgcaaattt
     1621 taacgcattt gccgacgaaa agataaaaca cataaaaata tacgcagata tatttaaaac
     1681 gtcttagaac gcaaaatttt acgtttaaaa gctatgcaag gactgtatga agcaaaatta
     1741 ttgagttatc caagaacaga tacaccattt attacagaga acgaatttgc ttatttaaaa
     1801 gcgaattttg gcaaatatag cggtttttta ggacttgatc ttgaaatggt tcaaacagag
     1861 cctagaaagc gttatgtgga cggtagtaag gtacaggaac accacgccat tatcccaaca
     1921 aaacaagtac ctaccgaatc tgcattagcg aaaatggacg atttacaacg aaaaatttat
     1981 gctttagtcg ttaaaacgac cgttgccatg tttttacctg attacttgta tgaagaaacc
     2041 aagatacaaa cgaaagtagc tgatttgctg tttcaatcga tcggaaagac accaaagcaa
     2101 gaagggtgga aaattctttt caaacaacaa accaaagaag aaaaagagga cgttcaaacg
     2161 ttaccactcg ttatcattgg cgaacgtgcc gaagttggtg ttaagagtgt tgaaaaagaa
     2221 acgcaaccgc caaaagcttt tacagagggt acattattaa ctgctatgaa aacggcgaat
     2281 aaaacggttg atgatgaaga agcaatcaag attttacaag aagttgaggg gattggaaca
     2341 gaagcgacaa gagcaagtat tattgaagcg ttaaaacaaa aagaatatat ccaagtgatt
     2401 aagaataagc ttgttgtaac tgaaaaagga aaattattgt gccaggcagt tgaaagtcag
     2461 caccttttaa cgagtgctga aatgacggct aaatgggaaa cgtatttaaa aaaaatcggt
     2521 aaaagagaag gcaatcaaga gaactttatt acgaatatca aaaaattcat tgttcattta
     2581 ctggaagctg tacctaacga tatagaaaaa ctaaattttt ctgattacca ggaacagaaa
     2641 gaaaaagaag cagaaaaaag tattgtagga aaatgtccta agtgtggcaa caatattgta
     2701 ttaaaaaaat cgttttatgg ttgttcaaat tatcctgaat gtaagtttac tttagctgaa
     2761 cattttagaa agaaaaaact aaccaaaacg aatgtaaaag aattactgga gggaaaagaa
     2821 accctggtaa aaggaatcaa aaacaaagag aaaaagccct acaatgccgt tgtaaaaatt
     2881 ggggaaaagg gatatattga ttttatctct ttttcaaaat aaacataaaa gccct
//
LOCUS       DAZKOB010000186         2929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000186 DAZKOB010000000
VERSION     DAZKOB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.194"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..981
                     /locus_tag="ACG8M6_002927"
     CDS             85..981
                     /locus_tag="ACG8M6_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0458502.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1073..1288
                     /locus_tag="ACG8M6_002928"
     CDS             1073..1288
                     /locus_tag="ACG8M6_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0458503.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1306..1578
                     /locus_tag="ACG8M6_002929"
     CDS             1306..1578
                     /locus_tag="ACG8M6_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0458504.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1580..2443
                     /locus_tag="ACG8M6_002930"
     CDS             1580..2443
                     /locus_tag="ACG8M6_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0458505.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1160 a          464 c          510 g          795 t
ORIGIN      
        1 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
       61 atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa agaatggggc
      121 gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat tttaaacaat
      181 aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg aaagtccaaa
      241 ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt aatgatcgat
      301 aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt agaattgcca
      361 cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat tgttcatttg
      421 actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc aaaattaact
      481 cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc acctttaaaa
      541 agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta tacaaataat
      601 gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga aagtacaaac
      661 aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa ccctggacta
      721 gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat aaaatcaaac
      781 ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa tattatcaag
      841 cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa aggctatgac
      901 aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg aattattcaa
      961 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     1021 atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt gagtgattgt
     1081 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
     1141 tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta taaaaattga
     1201 aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
     1261 acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc agttacgtat
     1321 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
     1381 ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact agaagtgaat
     1441 cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc atttgaagaa
     1501 ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca aattttgaca
     1561 aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc aatttgaaga
     1621 tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac
     1681 cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat cggcaatttc
     1741 cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc
     1801 taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg ttacccctta
     1861 ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt ataatttagt
     1921 gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa tgcttcaagc
     1981 caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg
     2041 aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca gggcaacacc
     2101 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     2161 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     2221 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     2281 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     2341 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct tgcagccacc
     2401 tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa aaaagtaaaa
     2461 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatttttta
     2521 aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt tggtacattt
     2581 tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc gaaaacacaa
     2641 gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt gcgttctcgt
     2701 cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa aaagaaagga
     2761 gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt cacttcctca
     2821 aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa gaggaaaaga
     2881 aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAZKOB010000187         2909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000187 DAZKOB010000000
VERSION     DAZKOB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.195"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            583..>2909
                     /gene="esp"
                     /locus_tag="ACG8M6_002931"
     CDS             583..>2909
                     /gene="esp"
                     /locus_tag="ACG8M6_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0458506.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1059 a          443 c          568 g          839 t
ORIGIN      
        1 gacaattcaa ttataaagaa aaagaatcaa taatttaatg aggctgtgac aaatgtcatt
       61 ttttgtttcg ctaaaaacga gtgaaaaaat gttgataact ccaatcattc gtttaagatt
      121 ctaggaatca ttcagaaatg ctgtgaaacc gatactttat aattataaag aaggtgattt
      181 caaattccga agaaggaatt tgaagtcacc tttttactta tgtcacagtc tcattcagta
      241 atgcgggata aggctcctta ttccgttatt tgaatactct gaagtttaat ctaagaaagg
      301 gtaatatttt gaaaacatgc ttcatcgcat agaaatgaac ttaatttcag aaagaataca
      361 tacatatctt tttatatgtt ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa
      421 aaacatgaag aaatgtactt tttttgttgt aatgttcatg aaattctcta agtaaattag
      481 ttaacataag gttagtaaga ttcctattct gtgaataata cgtatcagat ttatctttta
      541 ttcttactag gaaagtaatt attaagggag ttgatttgaa taatggttag caagaataat
      601 aagagagtat ttctagaaaa aacaaagaaa cgagtattga agtacagtat caaaaaatta
      661 agtgttgggg ttgcatctgt tttgattggt gttggtcttg tcttgggaac aactgaacta
      721 gttaaagcac aagatgaaat ttctccaagc agcccacttg aaacagcgat aagctccgta
      781 caagtaggtg acaaagtagc ttcggggaat acttttcagg agaatcctgg ttatacgaaa
      841 aattataact tttcggattt acaatttagt cctcaagaac taactggaga cacactaaaa
      901 ggcaatacaa ttggatttga agtttacgga aaacataata ttgcagcatc aactaaaaac
      961 tgggaaatcc gtcttcaatt agatgaacga ttagctaaat atgtagaaaa aattcaggtc
     1021 gatccaaaga aaggaattgg atcttctaga cggacctttg taagaattaa tgattcgctg
     1081 ggtagaccta caaatatttg gaaggtcaat tatattcgtg caagtgatgg attatttgct
     1141 ggggcagaaa caaccgatac gcaaaccgct cctaatgggg taattacgtt tgaaaagagc
     1201 ctagatgaaa tttttaaaga gataggcata gacaatctca agaccgatcg tttaatgtat
     1261 cgaatctact tggtaagtca tcaagacgat gataaaattg taccgggtat tgatagtacg
     1321 ggctactttt taacagattc agatgatttc tataatagct tagatgtatc tgaaaataat
     1381 cctgaccagt ttaaacatgg atcagtaagt gcaaaatatg agaaacctaa tactcaaaca
     1441 aaagatggtt caggatccac aggtgctaat ggagctataa tcttagatca taagttaacg
     1501 aaaaattata atttctcata ttcatcttcc gcaaagggca cgccttggta tgctaattac
     1561 aaaattgatg aacgattagt tccatatgta gcgggtatac agatgcacat ggttcaggct
     1621 gacaaagtaa catatgatgt ttcgtttgaa tccggaacaa aagtagctga tttagcaatc
     1681 gagcgacggg aaggtcatga aaattatggc ataggttcaa taactaataa tgatttaact
     1741 aaacttatcg attttgctaa tgcaagtcca cgtccagtcg ttattcgata tgttttacaa
     1801 ttgactaagc cattagatga aatcttagaa gatatgaaag caacagcaca agttgaagaa
     1861 aataaaccat ttggtgaaga tttcatcttt gattcttggt tgtcggatac gaataaaaaa
     1921 ttaatccaga acacttatgg aacaggttat tattatttgc aagatattga tggtgatgga
     1981 aaccctgacg ataaagaaga gagcggagac acgaatccat atatcgggaa acctgaatta
     2041 gaagaagtat atgatgttga cacaacagtt aaggggaaag tattcatcca cgagttagcg
     2101 ggaacaggtc acaaagccca acttgttgat aaagaaggta ctgtattagc agaaaaaact
     2161 atcgctccaa atgaaaaaga tggggctcca atttcagata ctgtagaatt tgaatttacg
     2221 ggtgtagatt caagtaaatt aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca
     2281 ggttttgata aaccagaaga aggttcaacc gttattaagg aatcaccaaa agcggttgat
     2341 aaacaaaccg tggtagttgg atttaaacca gatgctaaag aatcaattcg gaataataaa
     2401 aacttacctg aagatgcaga gtattcatgg aaaacagagc ctgatacttc taacgttact
     2461 gatagtacga aaggtattgt aactgttaag atcggaaatc gaactttcga cgtggatgta
     2521 gagtttgctg taaaagcttc tcaagctatg gaaaatgatg caacatacgt acctataaca
     2581 acaactccag aaacgacagt tcaaagtgga aaacctacat ttgataaacc agatgttcct
     2641 ttagctaaag atgccttttc aatttcagat gtctataata agtacttcgg taatgcaagt
     2701 gttgatgcaa acactggtat tgttacattc actccagcta aaggtgtagg agaatcggag
     2761 ccgattactg gaacaattcc tattaaaatt gtttaccaag atggttctgt aggcacgacc
     2821 gatttagcag taactgtaag taaagatatt tatgaaaatc caggagaaaa cattcctgca
     2881 ggctaccaca aagtaacctt caccgcagg
//
LOCUS       DAZKOB010000188         2873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000188 DAZKOB010000000
VERSION     DAZKOB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.196"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(504..788)
                     /locus_tag="ACG8M6_002932"
     CDS             complement(504..788)
                     /locus_tag="ACG8M6_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0458507.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(1307..1576)
                     /locus_tag="ACG8M6_002933"
                     /pseudo
     CDS             complement(1307..1576)
                     /locus_tag="ACG8M6_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            complement(1629..2801)
                     /locus_tag="ACG8M6_002934"
     CDS             complement(1629..2801)
                     /locus_tag="ACG8M6_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0458508.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          817 a          546 c          474 g         1036 t
ORIGIN      
        1 cttatctgat tttgtttcta agtagatatt tctcttttct aactctttct taaacgtttc
       61 tagtgtatag atatttgcta attttcttat ctccaataaa ctatttttta tataagtttt
      121 acattcatca tgattcatac aaactccacc ttctataaat gaatacaaaa aaagcaatca
      181 aacgatttcc gattgattgc ttaacaattc ttaaattcag tagcttagat acttgaaaac
      241 tctctgattt ccctatataa tgatagtacg gttatatacc gtcttcaaac aaagttaatt
      301 aaataacttc ttacgaggga agagttcatc tgactaactg ataagcgttg gtttggcaat
      361 cttatcgggc tatgcattta taaaatgtcg tcaaacattt tataaatgtg tcatggctct
      421 tttttcgttt ctattcagtt cgttgtttcg ttatatctag tataccgctt ttaaaaaaaa
      481 taagcaacga tttcgtgcat tattcacacg aagtcattgc ttttttcttc ttccatttct
      541 aaatccaatg ttacttgttc tgattctgtt tctggttctg gttctgttgg ctcatttggg
      601 attaaatcca ctactagcgt tgagttagtt aactttgcaa tttgttctag tgtttttatg
      661 gttggatctg attttcctga ttctattcgt gaataatttg atctactcat ttctaattct
      721 tgggagaatt tttcttttga tttggtacct ctcatctttt ttaagctttc tctaaatgct
      781 agttccaagt ttctcccccc tctttttgtg taataaaatt cacgctttat ttaaattata
      841 gcttttaata tcttaataaa caagtgatta atcacttgtg atttgtgatt attccaaaag
      901 taggtaattt caccaacctt tttcaaaagt ttttatcgac gattattgga ctggatcgtg
      961 tttcctaaag cgattctaag cgcatgttta attttaaagt gggtaattct ctttaaaacg
     1021 tcctacaaag attcaaaaaa agcatggctg ctagtggggg taccgtcagg aaaatgcgga
     1081 tttacaacgt taaggaaatt tattaaagag accaattcct cagcactaaa attgataatt
     1141 gggctttctt tgttactata gtgttaggta agctttttac tttatcaccg gttttcccct
     1201 cacttcacac ctacgggcga gtttccccac atcccgtttt ccatctaatc caattcattc
     1261 aatatcttta tgtgtatgaa agatcagatt gcttgattta gacatcttac acgtaattta
     1321 ttcttaaatg ggtacggtaa acgagcaata atacgagcaa aagtatacct acaaaaataa
     1381 taactctgtc taattttttt tgattcatat gcttattgct taaaccatat aatttcagtc
     1441 caagaaagcc tccaacagta tttgtaatta catctgttat gtctgtcgct ccaatagcga
     1501 agataaattg aattatttca aaagtaagac ttaaaaccag tacaaaagca aacttaggta
     1561 aaaatccgat ttctttaagt caagtccata atcctgtgta aaatactata caatgtttta
     1621 ccggtctctc attgatcaaa cttaatatat tttcttgtag aactgagcca ttcgtcatga
     1681 aggtccataa ggacagctcc aattaatcga ttggcagacg ttcggttggg aaaaatccga
     1741 ataatctttt ctcttctgcg gacttcctgg ttcagtcgtt caagaaggtt ggtgcttttt
     1801 agccgattat gactatttcc gataaccgtg tattgaaagg catcttcgaa gccattatcc
     1861 aatatttcgc aggcttttgt atatttaggc tggtcgatat attcaccgac taaggcattc
     1921 ttagctgttc gagcgagttc aatatccgta aacttaaaga tcgcttttac tgcttctcta
     1981 aacggttttg aattcttttt tggaatggaa ctgaagatgt ttcttaaaaa atggacctgg
     2041 catctctgcc aacttgcgtt ggtaaatgac ttacgaatcg cagacactag gcctttatgg
     2101 gcatcagaaa tgatgagttc tgtccccttt aggccgcgtt cttttaagta ttcaaagaag
     2161 atggaccatg tgtcatcact ttcttcattt tgaatcatga agccaatgat ttcacggtcg
     2221 ccaccttctg ttatcccgat cgcaatatgg cagcttttag aaagcactcg atggtcctca
     2281 cggactttta tgtagagaac atcagtcatc agataaggat aattcgtacc tgagagtgaa
     2341 cggttctgcc attcgttgac catcgggtct aactgctcag tcaggctaga aacaaaagat
     2401 ttcgatacag attttccaca tagctcttca acaatctttg aaactttacg tgtcgaaacg
     2461 cccgaaacat acatctcaag cattgaagcg agcagtgcct tttcatttcg ctgataacgc
     2521 tcaaacaccg tcggtgaaaa ttcaccatca cgtgttctag gcacttttaa ttcgagtgta
     2581 cccacacgag tcgtaaagtc tcgctcataa tagccatttc tttgactctg acgactttct
     2641 gaacgttcat agtcatcggc ttgaatatat tctgttcgtt gattttccat caattggttg
     2701 aaaacagtgg ttaaaatatt tttagaaaca tcatctttta ccgaatgttc aataatactt
     2761 tgaacctctt cgttgttcag tgtaaaatgt acttgggtca tgtaacgtcc tcctgggtat
     2821 gtttttgtgg ttaaaaacat tgtaccgtaa aagggctgtt acatggcctt tta
//
LOCUS       DAZKOB010000189         2821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000189 DAZKOB010000000
VERSION     DAZKOB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.197"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            188..511
                     /locus_tag="ACG8M6_002935"
     CDS             188..511
                     /locus_tag="ACG8M6_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458509.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            675..929
                     /locus_tag="ACG8M6_002936"
     CDS             675..929
                     /locus_tag="ACG8M6_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458510.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            999..1244
                     /locus_tag="ACG8M6_002937"
     CDS             999..1244
                     /locus_tag="ACG8M6_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0458511.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1320..1685
                     /locus_tag="ACG8M6_002938"
     CDS             1320..1685
                     /locus_tag="ACG8M6_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0458512.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1746..2309)
                     /locus_tag="ACG8M6_002939"
     CDS             complement(1746..2309)
                     /locus_tag="ACG8M6_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458513.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          835 a          493 c          432 g         1061 t
ORIGIN      
        1 catcataaat aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca
       61 caagtttcaa aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata
      121 actattttgt tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt
      181 attctcaatg ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc
      241 ggcagcagct tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat
      301 gatttttcaa ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa
      361 aagatatgcc cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat
      421 catcattctt tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt
      481 agatatcaat ccattgattt tttctcgata gataagacac attcagcgga acagttcctg
      541 cttatcagca gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt
      601 tcctagcatt tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt
      661 gaggagtccg ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac
      721 tggtcgcaag tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt
      781 tgactcttcc ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg
      841 atagtgtttt tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt
      901 atttgatacg tttaaataaa aaacattgag tactattgat tgcatgtgta gctttctata
      961 gtcattataa ctattctact ataatgaaag gtgattaaat gagtatcgaa gaagctattt
     1021 tactggatac tttacgttgt cgtctgaatt tcttaagaac tggccgatct ttatctcaag
     1081 tagctgtttc tagcgcgtta agtattcctt tattcaaata tcgtaagtac gaagaaggag
     1141 aagaaattcc aagcatcatt gaattaatct atatcgctga cttttacgat atttccgttg
     1201 attacctaat tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt ccagccttgg
     1261 tgctggtttt ttcatttaac gtctaaggtt aacaatctat atataaagga gacatttcta
     1321 tgcgattatg gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt ttaggacaac
     1381 atcgggaatg ctgtgcatta agaggaaatg gctggggaaa gaaacatgct actgtggatt
     1441 atgtttttca ctattctcct tatagattat tccaatatca tcagcttgtc atggatgaaa
     1501 tggaaaaacg tggttacagc ccagcaccag agtggaaaaa tcctatgtat cgtggcaaat
     1561 catgcgaagc attttttgat ttacctactg tagagcgcaa cgtgccgatt tatccggaac
     1621 acgatgaagt ttatctagca gaatgtttag caaatttgga gcaaaaagga atttatctta
     1681 cctaatataa ctagaaaagc cttctatctc ctcttacttc attaaaaagc tttaaccatc
     1741 ttagatcaag ctaaaatgtc gtaagtttta gggttatctt catcctcacg tgcaaaacta
     1801 aaagcgtact ctagttttgg ttcatcgcca acttccctag acataaatga acatctcctg
     1861 acgtggttta aaagtaccgc atcttcgacg tctgtatacg atgcacagca cagattgctt
     1921 cttttgatct ttcttttgat ttttctgttg cttcttctac accttcttcg attactacat
     1981 cgatgcttac aattgcttct gcctaatatt cccatgcatc gtcgatttaa gcctctgtag
     2041 tcactgtgat cgtttccaca ttttaactcc tctgtcgtgg cttcagttga ttttgtgatc
     2101 agttcagttg ctaagccaga gtcagaacaa cctgccattg ctagcgttaa tattgatagt
     2161 aaaaatagtt tttttatcat aaggttcccc ccctgaaaat agactaaaat aataacgctt
     2221 tctttttttg ttttcagacg aaaattaata atttctaaat attctttaaa aaagcttata
     2281 actattatgc cttttttaat atataacaca gttattttta aaaataagaa tgctaaatca
     2341 ctctacaata acggcgaaag cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag
     2401 atgaaagatc aggttttatt agatctcatt ctttcatcac ttgaagggat ttaatgaact
     2461 actttttcct ctttgtcgtt tttatatatt cgtattcctt caaacagtag gatagcctct
     2521 ttcaatgctt gatatttgaa ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc
     2581 tagaacatat tcttttttac tttttcatta attgacagac caatcgaacg aaactgtaaa
     2641 tttcgatagg ataatgattg gtcaaactca ttcattttca agacatctcg attcatattc
     2701 aagataggaa tctttttatc aacatcactt tattcgtatt ttcctctacc ataaaagctt
     2761 cacgctccac aaaattgttt tatacactac tattcagccc attcaacgac caatgaattt
     2821 t
//
LOCUS       DAZKOB010000190         2802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000190 DAZKOB010000000
VERSION     DAZKOB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.198"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG8M6_002940"
     CDS             85..972
                     /locus_tag="ACG8M6_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0458514.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8M6_002941"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG8M6_002941"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0458515.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG8M6_002942"
     CDS             2274..2720
                     /locus_tag="ACG8M6_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0458516.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          936 a          451 c          609 g          806 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt at
//
LOCUS       DAZKOB010000191         2701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000191 DAZKOB010000000
VERSION     DAZKOB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.199"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..515
                     /locus_tag="ACG8M6_002943"
     CDS             171..515
                     /locus_tag="ACG8M6_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0458517.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="ACG8M6_002944"
                     /pseudo
     CDS             516..935
                     /locus_tag="ACG8M6_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="ACG8M6_002945"
     CDS             complement(1027..1377)
                     /locus_tag="ACG8M6_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458518.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="ACG8M6_002946"
     CDS             complement(1343..2674)
                     /locus_tag="ACG8M6_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0458519.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT          999 a          489 c          522 g          691 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 a
//
LOCUS       DAZKOB010000192         2619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000192 DAZKOB010000000
VERSION     DAZKOB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.200"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..317)
                     /locus_tag="ACG8M6_002947"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="ACG8M6_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(349..672)
                     /locus_tag="ACG8M6_002948"
     CDS             complement(349..672)
                     /locus_tag="ACG8M6_002948"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0458520.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
     gene            1134..2516
                     /locus_tag="ACG8M6_002949"
                     /pseudo
     CDS             1134..2516
                     /locus_tag="ACG8M6_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          940 a          456 c          433 g          790 t
ORIGIN      
        1 tttcgtaacc agatatctaa ggttaagccg tccgcatcaa ttgcacgata aagataatgc
       61 caacgtccct taattttgat ataggtttcg tccattttcc atgaatagaa ggattgtcta
      121 tttttcttct tccaaagata atagaggact ttgctgtact cttgtaccca acgataaatc
      181 gtagtatgac aaacatttat tccacgatca tatcacaatt cctgaacttc acgatagctt
      241 agattgtaac gcaggtagta accaacagcg acaataatga cgtctttttt gaattgtttg
      301 cctttaaaat gattcattac tctgtcctct ctgtcttttt tctcaatttt acactaaaat
      361 agattttttg gaaaactttg caacagaacc gaaatgagta tagtatgaat tagtaatttg
      421 gttttcatct aagtttagct ttgatttact aaaattgata tctcgataca tgttcaatac
      481 atctaataca aatcgagaat cttctttcgg aaatggctca gaaagatcac tccaaatttc
      541 atcatcataa tgatactcgt aaccttctct caatatttcg atttttcttt cataataaga
      601 tctgttttgg tcctcaaatt tagccaataa ctcaaattga tttactagtg acaatctctg
      661 aaaactgtcc attaaatgtt tcttcctatc ttgttcatta acaaaaaatt ataagccctt
      721 acaacgaata ttatatatta aatttaattg taggtgaaca cgaattttat aaatatttat
      781 ggtgttaatg acaaatgata tccgactcct cttcatacgt tctggactat ttgctaatag
      841 attaaaattt cagcatctat ttctgaccat aaataaatga gctgccgaaa agctgccaaa
      901 tcagacttga aaataaagga tcaaaaactt ttaagtttct gaccccgtaa ctcacacttc
      961 aaaatcttac ttaaaaagga gtctgaaaga aaaaaagagt attcaaaatt tttttctaac
     1021 tagctacatc acatctatgc attgacactc aaagaaccag aataagtcat cagaaagcaa
     1081 gggggtgcgg acataatcct ggacgaaaaa ataaacttag gaggaatgtc cgtgtgtttt
     1141 cccacaaaga gcgtgtaagg gcaattcagt tatttttaaa atacgattgc tcttatgctg
     1201 caaccattcg agaattaggg tatccgtcga ttggtgcact acggaaatgg tataaagagt
     1261 atttggaatc cggagagctc catcaggagc accgaaaaaa atcaaagtat tctgaagaac
     1321 aaaaacgaat agcggtgaat cattattttg aatatggtca atgttatgca cgaaccattc
     1381 gcatgcttgg ttatcctaat agagagtcct tgcggcagcg gtgtgaagaa cttgcaccag
     1441 gagctagaaa actccgaaaa agtgcggtaa agttaacaca agatcaaaaa gaagctgtgt
     1501 tgaaaaaatt ctataaaccg caaactaata ggaaaaattt agccgaatct ggaggaatta
     1561 gtagagttac tctatatcaa tggaaaaaca cggttcttgg aaaggatttt ccattgtgta
     1621 tgactttaaa aaatcaggaa aaacaaaaag agctactttt aaaggaagta gaagacttac
     1681 aaaaacaagt acaccaatta cagctcgaaa aagcattact cgaaggagcg gctgaactgc
     1741 taaaaaaaga aaaaggcgtc aatcttcttt gtctatcaaa tcaagaaaaa acgatattga
     1801 ttgatgcctt acgaaatcag ttcaccttga aagagcttct tcaacagctc cagtttccta
     1861 aaagcagcta tttttatcaa aaacaagcac ttgagaaacc agataagtat tacaaagaac
     1921 gtcaaattaa ttatcacgat tttcaatcat aacttctgtg cgtatggtta ccgtagaatt
     1981 caccaagctt taaaaaatat gggtaaaaag ctttctgaaa aagttgtacg tagattgatg
     2041 actgaggaaa atctatttgt taaattttct agacgaaaaa agtattcttc gtatgctggg
     2101 gaaatttctc ctgcacaccc caatttatta aatcggaatt ttaaagcaaa gaagccaaat
     2161 gaaaaatggt taacagatat tactgaattt agaattctcg cagggaaaat ctacttatca
     2221 ccacttgtcg attgttacga tggtgctatt gttagttgga ctattggaac aaagccaact
     2281 tgcttcactc tatgtctaaa aagagttatt caccagataa ttcagcgtgc gaaggtttct
     2341 ttggacgatt aaaaaacgag atgttttatc aacgtgactg gaagaataca acaatcaatc
     2401 agtttataaa caaattggat gattatattc attggtataa taatcaacga attaagttat
     2461 ctttaggaag gctaagccct cttcaatatc ggaagaagca aagatatatt tgttaaaaag
     2521 gtccaaaaaa aatatccgca cccccttttt ttgtaaaaaa ctattgcaca atacaagtag
     2581 cctacggcta tcgaaatttc cataactttc gagcaagaa
//
LOCUS       DAZKOB010000193         2582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000193 DAZKOB010000000
VERSION     DAZKOB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.201"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..340
                     /gene="relB"
                     /locus_tag="ACG8M6_002950"
     CDS             119..340
                     /gene="relB"
                     /locus_tag="ACG8M6_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0458521.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            342..608
                     /locus_tag="ACG8M6_002951"
     CDS             342..608
                     /locus_tag="ACG8M6_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0458522.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            775..1260
                     /locus_tag="ACG8M6_002952"
     CDS             775..1260
                     /locus_tag="ACG8M6_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458523.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVGTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKLQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     T"
     gene            1469..1651
                     /locus_tag="ACG8M6_002953"
     CDS             1469..1651
                     /locus_tag="ACG8M6_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0458524.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            1754..2038
                     /locus_tag="ACG8M6_002954"
     CDS             1754..2038
                     /locus_tag="ACG8M6_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458525.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            2054..2290
                     /locus_tag="ACG8M6_002955"
     CDS             2054..2290
                     /locus_tag="ACG8M6_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458526.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            2312..2566
                     /locus_tag="ACG8M6_002956"
     CDS             2312..2566
                     /locus_tag="ACG8M6_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458527.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
BASE COUNT          915 a          409 c          474 g          784 t
ORIGIN      
        1 ttctacaaca taaacatata tatgtatatg tttatgttta tgtatatatt caaatatata
       61 actattgcat ttgtcataca atcgtagtac aatgtaattg aaaaggagtt gataaactat
      121 gccagtaata agtattcgtg tatctaaaga agaaaaagat tttcttgaaa aagttgctga
      181 attcaacgga gataacattt ctgaatttat gcgtagtaat tccctgcaaa cagcagaaac
      241 gttagtagac tttgccacct acaagcaact aatgaaagag catgaggaaa acgatcaaag
      301 tatttcccac gaagatatgc taaaagagtt aggcttataa gatgacttac caagtaagat
      361 atgaacgtga agctcaaaaa gcgctaaaaa aaatggataa gtttcaggca aaggttatcc
      421 taaactggat cgagaaaaat cttgtaggca ctgatgatcc aagaaagcat ggtaaaggtc
      481 taacagctaa taaatcaggc tactggcgat atagggtagg tgcataccgt ataatagctg
      541 atattagcga gcatgaagtg acaattctta tattgtctat tggtcacaga agtgaagttt
      601 ataagtaaca gacaagctga taaggcttgt ctgttttttt gttaacccaa caaaatcact
      661 gtttacgatc ccactttacc taaaaaagaa caatatcctc ttgatattat acacaccagt
      721 gtgtataata taagtgtaaa caagctaatt aactttcaaa aggagcgatt ttttatggga
      781 tggtatggtg attattcatc agcaaatgaa gtgatcgaag aaaagaaagg gtacgccaaa
      841 aatggtttgg aaatcgtagg taccaaagta acgaagacct atggaattat ccttttcaaa
      901 gatgcagaag gaacctacga tattgacagc ttcatctttc gagatgaaat gtacaaacca
      961 atctacttct taaatgacag tcctgcttac tttaagaaaa ttccgaaaaa atggttgaaa
     1021 ttctttgtcg gtgatcaaga aaaaatggtt aagctacaac tagatagaat taaagaagaa
     1081 agcaataaac caaaacttga tgacttatta gttgcaggta aaacttactc aatctgggat
     1141 ggatcgtatg tcgctgtcta tcagtataaa ctcaaacgta gctttatttt tcgactacaa
     1201 gatggatcac tcactcggtt cactcgacta tcagcaggag atgttaagga agtaacctaa
     1261 aacttaaata cataaacata aacgtgtata cgtttatgtt tatgtatatg tatatgaata
     1321 taaacgtatt aggtacgaac acttttaaat tcttttctgg gctttgtttt tagggtaggg
     1381 ctaaaaaaaa catcaccatc tgatccaaaa cagctcagaa acgcatttaa acgtatctga
     1441 taccaatatt acagaaggag tgtatcgtat gattaaccta tttcttttag tgacagctag
     1501 tacattattc tttactggaa tatcactgat aatttcctat ttgatctata agaaagccga
     1561 aaatcacaca agcaaagcaa tcggactagc cgtttcatcc tttttaactt tgctgacttt
     1621 atttgttact ttatcgtttt cttttgttta gaacagctga tccattagca cctttttctc
     1681 tttacattat acacactgtt gtgtataatg tatttgtaaa caagctaatt aacttttagg
     1741 aaggagtgac aagatgctaa cagctacttt acagcaacct actcacgttg ctattgcaga
     1801 aagttataca attaatgcag ggatcgttaa acagctgatc aaggatcgag aattcaaaaa
     1861 gtttaacagt cggcaagcgt ataacagaca gctaaagaaa gctattctaa agcacaaaga
     1921 ttccttcatc gggaacttgg aaagtgtaga tgttaaaact gtttcaacgg cattttcagt
     1981 tgttgaagtg actattgacg gtcgcttgat ttctctattt gcttttggaa ttaattgata
     2041 gggagtgtga actttggaag gtaagacttt tgcagaaatt tggactgaat ttattgaggt
     2101 accaactcgc attgatccga taacttctga aaaggtattg gatgcagact tctacaatct
     2161 caaagcaggt actagccttg aatgcgtttg gatgtattta gaagagcatt tcaaaggcgt
     2221 aacgctcggt cacgtcttat accacaatgg tgccgagtgg gatgcaattg gaaatatcca
     2281 tttttcttaa tataagaaag gagaacacaa tatgaattat accgatgaag aacaaatcgc
     2341 tttggaatac gttttcacaa atgaagaagg ggaggaaatt gaaaaagtct tgatcatttc
     2401 atcaaaagac ttggagaagg ctttggctta tgaaccatac gaatcaaccg taactgtcat
     2461 tgaattctta gatacctata cttctaatga tacttgggaa attatgaata tttgcaacgc
     2521 taataacatt caatttgctg aaaaaaccaa taaatcattt tattagtttt gcaataaaac
     2581 at
//
LOCUS       DAZKOB010000194         2579 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000194 DAZKOB010000000
VERSION     DAZKOB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.202"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..2514
                     /locus_tag="ACG8M6_002957"
     CDS             73..2514
                     /locus_tag="ACG8M6_002957"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0458528.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          861 a          460 c          610 g          648 t
ORIGIN      
        1 aaaaaataac tggaacaaga gctatttgtt tttacagcaa agaatggtat atttttgtag
       61 gaggtggctt acttgaaaga agtcgttatg atagatgctg caagaacgcc gataggaaag
      121 tatcgcggaa gtttaagtcc atttactgca gttgaattag gcacacttgt gactaaagga
      181 ttattagata aaacaaaact gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc
      241 ctgcaagctg gtaatggtca aaatgttgcc agacagattg ctttgaacag cggtctccct
      301 gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc
      361 ttagcacgcc agctgatcca attaggtgaa gcagaacttg tgatagccgg cggaacagag
      421 agtatgtctc aagcacctat gctgaaacag tatcagtcag aaacaaatga atatggtgaa
      481 ccaatttcca gtatggtcaa cgacggattg actgacgcat tttcaaatgc acatatggga
      541 ttaaccgcag agaatgttgc aacacaattt tctgtgagca gagaagagca ggatcgctat
      601 gccttgtcgt cccagttgaa agcagcacat gctgtcgaag ccggtgtatt ttctgaggag
      661 atcatcccag tcaagatttc tgatgaagac gtgttatctg aggatgaagc agttcgtgga
      721 aatagtacat tggaaaaact gggcacgtta cgtacagtat tctcagaaga aggaactgta
      781 acagcaggaa atgcttcccc gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa
      841 gaatacgcag aaaataataa tctgccttat ttagcaacca tcaaagaagt agcggaggtc
      901 ggtattgacc caagtatcat gggaatcgct ccaatcaaag ccattcaaaa attgacagat
      961 agatcaggaa tgaatttatc aacaatcgat ctttttgaga tcaatgaagc atttgcagct
     1021 tcttctatcg tagttagcca agaactgcag ttagatgaag aaaaggtcaa tatatatgga
     1081 ggagcaatcg cattaggcca tccaattggt gcaagcggtg cacgtatctt gactacatta
     1141 gcttacggat tgcttcgtga acagaagaga tacggtattg cttcattatg tattggtgga
     1201 ggtttaggct tggcagtctt gttagaagcg aatatggagc aaacacataa ggatgtgcaa
     1261 aaaaaaaagt tttatcagct tacgccatct gaacggcgca gtcaattaat agaaaaaaat
     1321 gtattgacac aagaaactgc gttgattttt caggaacaaa cactttctga agagctctcc
     1381 gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc caatggggat cgcgcagaat
     1441 tttcaaatca atggcaagaa aaaatggatt ccgatggcaa cagaggaacc atctgtcatt
     1501 gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg
     1561 ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt atcaagcagt gatcaatgca
     1621 gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg
     1681 aaacgcgggg gcggtgttca agatatctct actcgggaat tcatggggag tttccatgca
     1741 tacttatcta ttgatttttt agtggatgta aaagatgcaa tgggcgctaa catgatcaac
     1801 tcgattcttg agagtgtggc aaacaagtta cgggagtggt tccctgaaga agagattcta
     1861 ttcagtattt taagtaattt tgctacagaa tcattggctt ctgcctgttg tgaaatccca
     1921 ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag
     1981 gcaggggaat atgcgaagct tgacccttac cgtgcagcaa cgcacaacaa agggatcatg
     2041 aatggtatag aagctgtagt ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg
     2101 atccacgcct acgcggcaag aaatggatta taccaaggat taacagactg gcagatcaaa
     2161 ggggataaac tagttggaaa actaacggtg cctttggctg ttgccacagt aggcggagct
     2221 tccaatatcc tgccaaaagc aaaagcatct ttagctatgt tagatattga ttcagccaag
     2281 gaactagcac aagtaattgc tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca
     2341 ttagttacag aaggaatcca aaaaggccac atgggacttc aagctcgttc attagcgatc
     2401 tcaattgggg ctattggtga ggaaatcgag caagttgcga aaaaattacg tgaagcagaa
     2461 aagatgaacc agcagacagc tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc
     2521 aatatcttta gaagaataaa gcaagattta cagttatttt atttactgca gatcttgct
//
LOCUS       DAZKOB010000195         2577 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000195 DAZKOB010000000
VERSION     DAZKOB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.203"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG8M6_002958"
     CDS             <1..1412
                     /locus_tag="ACG8M6_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0458529.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2577
                     /locus_tag="ACG8M6_002959"
     CDS             1546..>2577
                     /locus_tag="ACG8M6_002959"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0458530.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNL"
BASE COUNT         1010 a          360 c          518 g          689 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaattta
//
LOCUS       DAZKOB010000196         2558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000196 DAZKOB010000000
VERSION     DAZKOB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.204"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..727
                     /locus_tag="ACG8M6_002960"
     CDS             92..727
                     /locus_tag="ACG8M6_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0458531.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            840..1475
                     /locus_tag="ACG8M6_002961"
     CDS             840..1475
                     /locus_tag="ACG8M6_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458532.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(1468..1602)
                     /locus_tag="ACG8M6_002962"
     CDS             complement(1468..1602)
                     /locus_tag="ACG8M6_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458533.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            1774..>2558
                     /locus_tag="ACG8M6_002963"
     CDS             1774..>2558
                     /locus_tag="ACG8M6_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0458534.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKK"
BASE COUNT         1011 a          379 c          436 g          732 t
ORIGIN      
        1 agggattatt aatcaagaag aattcgattt taagaaaaaa gaattgcttg gtttataata
       61 ttcaatatca aaagagaaag aggatctatt aatggacggt atatttggtt ttactggtat
      121 tgttttattt attataggtt taatcatgtt aattgtaaga tttatcaaaa aaacaaataa
      181 gaaaacacct atgattctcc ttattatcgg gattattttt actaccgtag gtttctcgct
      241 ttcctcatca aacgaggcaa atgacaataa caaacaggaa agctcatcat ctgatactca
      301 aactactaca tctaacagca gcaataaaag cctaactcaa tttgaagaat ttttagagac
      361 aaataatcat gactggggtg ctttcttaga tagttattat tctattactc cagctactga
      421 gcaaaataca gcttttacta aatatattag tggtaaaaca tatacttttg aaggaactgt
      481 tatcgaatca atgactacta gaatagccat tatagcagac aaagagtacg atgataaaag
      541 ttggaatgat atttctgcaa cacctaaagt ttcatatgta atttttgcga aggacttaaa
      601 caatgctgat acctttacaa agggcgataa agtaactttt acaggagaaa ttagttcaag
      661 aggatcgaac atagaaaaat cctatgctca atgggatatg attaatagta aagtaagtaa
      721 aaaataaatg aaccacgccc cactaagagt tagattgtgc acaataataa aacctataca
      781 aaagagtgcc atcaggagtc acaatgcttg taaaggaaat gacaaaatag gaggtaacta
      841 tggataatag caatgaaaaa ctaaccaaaa aagaatatct tgaattaata gttgaaggcg
      901 gtattggagc aattccttat attggtggcg ttgtgcagtc actttatttt gggtctaaaa
      961 acgagaagcg tttcaaacgc atcgagaaat tctacaaaaa tttaaacgaa tcattagaaa
     1021 acattcgcga tcaaattcca gaagatgttt tcaacttaga gaacaaggac caactcattg
     1081 gaatattcga agggataaat gacgaggtag aaaaatcaaa ggcacaaaat aaaatagata
     1141 tgtataaaaa tctttataag aattgtttac ttcgaattaa caacgcttca tgggataacg
     1201 aggagtatta tcttcttgta ctgaatcaat taacatcgat tgagattcaa ttgttagcat
     1261 gcttaatgag tcgtggaaac gaaaatttca ctggaaatat cagcatgtct ggctattcac
     1321 aagagctaat agatggtagc ttaaatagat tatctgattt tggtctgtta gaaaaaacga
     1381 ttagctccat agtattaggt gatgtcggca aacaaaatat gggctacaaa atatctcatt
     1441 taggaattca atttattaac tcaactctta cttaagtttt ctttctaata tgacatctag
     1501 acctatcagc aatgcaacaa taaaaaacca gacataccaa taatcaacaa ataatctaaa
     1561 ctctggaagt tcagttattc ttttaaaaat cactttaatc ataataaaca cctctgattg
     1621 gatgatctaa tacacatcca attataccat taaaacgaca aatagcacat ccttgcgccg
     1681 gcaagcaaaa aggatgtgca aaaaataata aaacccaaac aatgggcttc tttatagttc
     1741 ctattgtatc agagaaagag agctgattca attatggcaa gattagtcaa acgtggaaat
     1801 agttggcaat acgaaatttc atacaaaaaa gatgacggaa aatacacgaa gataagaaaa
     1861 tcaggattta agacaaaagg agaagcaaaa gatgccgcca acgaattaga atataacttg
     1921 aacaaaggcc ttaaagggga tcgcaaaaat ctattattat cagattactt tgaggattgg
     1981 atgcaacttt ataaagaagg aacagtatct cctatcactt atagaaaata cgaagatacg
     2041 ttaatgaaca taaagaaata tatgccagcg gtattgattt ctgatttaga tagagtggga
     2101 tatcaacgct ttttaaataa gtatgctaaa gaccatgtaa aatctaccgt tattaagttt
     2161 aataaccata ttagagcgtc gttgaaagat gccgtagaag aaggattaat tccgtttgat
     2221 ccaactagaa aagcagtaat caaaggaaaa gattcattga agccaaaaga agataaatat
     2281 ttagattatg atcaatttaa atctttaatg aaactcgtag aagaaaacct ttctgcgcag
     2341 tactcttctc ctatgctcgt gttagttgct ggtgccactg gaatgcgatt tgctgaactt
     2401 ctaggattaa catgggaaga tatcgatttc gaagatcaaa tcatcactat taataaaaca
     2461 tggaattata aattaaatga atggggaaaa acaaaaaacg aaacttcaaa taggaaaatt
     2521 tccattgata aacatacgat tgatctctta aaaaagtt
//
LOCUS       DAZKOB010000197         2530 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000197 DAZKOB010000000
VERSION     DAZKOB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.205"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            232..2385
                     /locus_tag="ACG8M6_002964"
     CDS             232..2385
                     /locus_tag="ACG8M6_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0458535.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          870 a          436 c          552 g          672 t
ORIGIN      
        1 tgatccatca aagtgtttca cgacttaaga aaacataatt ttcaaaaata agtttatttt
       61 ttaatgaaaa aaatagttgg atatgtaact ttaagtgatt gctgtgaaac aaaaagttga
      121 ttttatcttg aaattaggta aagaatttct tgctccgccc attttttctt ttttattaag
      181 aagtttttgg gtacaatgaa gcatgaatgt aaataggcgg aaggatagga aatgatgaaa
      241 gattttatga aaaaaatatt aaatacttct tggttagaaa aaataagaaa aggtgcacaa
      301 gaacctaaga acaaaacgtt tcgccggtcc catgttcctt ttcgcttaaa ttttcttttt
      361 ttcattatat ttacgttatt tgttgcgttg attgctagat tgggttattt gcagatcgtg
      421 aacggcgaag agatggaggc gcgagtgaag tctacttcta cgattacggt ccaagaaagt
      481 actcctcgcg gaatgatcta cgactccaca ggaaaagctt tagttacgaa taaagcgaat
      541 caggccatca catatacgag aggaacacaa gttacggcta aagatttgct tgaaatcgca
      601 acgaagctga actcgctgat cgatgttccg gtagatgaaa atctgacaga tagagataaa
      661 aaagattttt ggctggctga tcccaaacat ttgaaagaag ccacaaaaag actttcggca
      721 aaagaaaagc aattagggaa cagcgaacaa tatagtgcga tggtcaataa agtcacagct
      781 gatgagatta atttaaacga ggagcaatta aaaattgcta ccatctttaa acggatgaat
      841 agtgcatatg aattaaacac tgtctatatc aaaaactctg gtgttactga tcaagagtta
      901 gctgttgtgg cagaacgcgc ttctgaactt ccaggagttt cgactggcac agactggaca
      961 cgagaatata atgcagcaag ttcactgaaa agtattcttg gcagtgtgac gactgaaaag
     1021 caaggacttc cagcagatga agcagagaaa tacctagcaa aaggttattc tcggaatgac
     1081 cgtgtaggac aaagttattt ggaaaaacaa tatgaagatg tcctgcaagg aaccaaaacc
     1141 caatatgaag tttcccttga taatgaagga aatgtttcga atcaaaaaga aatcttctct
     1201 ggggaaaaag gttctaattt aatgttatct atgaatgcag aattccaatc aaaagtagaa
     1261 gaaattttga aaaggaatta tcaaacattg atcaacaacg gcaaagcaca gtattcgcct
     1321 ggtgcttatg cagtggctat gaatcctcag acaggagaag tgctagcaat gactggtttc
     1381 agtcatgaac aaggttccaa agaaatcacc gagaatgctt taggcacgat tactagcgcc
     1441 tttgatccag ggtctgtagt caaggctggt actttgacag caggctgggc aagtaacgcg
     1501 atcagcggaa atcaagtttt gattgatgag ccgattcgtc tgcaaggtgc ttcagaaaaa
     1561 agctctgtct ttaatcgaag cggacaagtt gctcttgatg cagtcaaagc atcggaattg
     1621 tcttctaata cgtatatgat caaagtggcg ttaaagatgt taggactaga ctatacacct
     1681 ggaatgggac tacctagttt agatgaagaa gctaaagctt atcagcaatt gagagactct
     1741 ttcaaagagt ttggtcttgg tacaacgact gggatcgatt tgccgaatga atctcctggg
     1801 atctcacgtt ctgttgatta tatgaagaag ttcaatgcgg ataatggcaa agaatggtat
     1861 gctccaggta actttaccga tttggcattt ggacagtttg acacatatac accaatccaa
     1921 ttagctcaat atgcttcaac agtcgctaat ggcgggaaac gagtacagcc gcacctagtc
     1981 aaagcgatct atggcaatga cgaaaatgga aacttgggtg aagtgaagaa agaaattggc
     2041 acaacagtag aaaatacggt caatatttct gcagaaaata tggcgatcct ccgtgaagga
     2101 ttccaccaag ttgtccatgg taccgatcct tacacaacag ccaaaccatt agccagtgcg
     2161 aaaatggacc tttccgcaaa aacagggact gcagaaacgg ttgcggaagg tcatccagat
     2221 attgcgacag tcaatagtaa tatcgtggca tacggaccaa cagacaatcc ggaaattgcc
     2281 atcagtgttg tcttgccgaa cttattagac gaacaagacc acataaactt gacgattgct
     2341 aaagaaataa tggacgcgta ttatgatatg ttcatggcaa aataaaaaag aggtcgtgaa
     2401 aaagccgctt agtttcgagt atgaagaaaa attttgtgaa aatagcttct cacatttttg
     2461 cttaaatttg acttcatacc gagaaactgg cttttgaaca ccgtttactc ggcaaaaaag
     2521 gttgtgacaa
//
LOCUS       DAZKOB010000198         2400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000198 DAZKOB010000000
VERSION     DAZKOB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.206"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG8M6_002965"
     CDS             207..2069
                     /locus_tag="ACG8M6_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0458536.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACG8M6_002966"
     CDS             2069..>2400
                     /locus_tag="ACG8M6_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0458537.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZKOB010000199         2395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000199 DAZKOB010000000
VERSION     DAZKOB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.207"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG8M6_002967"
     CDS             316..570
                     /locus_tag="ACG8M6_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0458538.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG8M6_002968"
     CDS             571..888
                     /locus_tag="ACG8M6_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0458539.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG8M6_002969"
     CDS             1447..2382
                     /locus_tag="ACG8M6_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458540.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZKOB010000200         2394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000200 DAZKOB010000000
VERSION     DAZKOB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.208"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG8M6_002970"
     CDS             complement(573..1130)
                     /locus_tag="ACG8M6_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458541.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG8M6_002971"
     CDS             complement(1309..1920)
                     /locus_tag="ACG8M6_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458542.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG8M6_002972"
     CDS             complement(2033..2290)
                     /locus_tag="ACG8M6_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458543.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
BASE COUNT          696 a          517 c          317 g          864 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aata
//
LOCUS       DAZKOB010000201         2376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000201 DAZKOB010000000
VERSION     DAZKOB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.209"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(253..621)
                     /locus_tag="ACG8M6_002973"
     CDS             complement(253..621)
                     /locus_tag="ACG8M6_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0458544.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            771..1253
                     /locus_tag="ACG8M6_002974"
     CDS             771..1253
                     /locus_tag="ACG8M6_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0458545.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1290..2300
                     /locus_tag="ACG8M6_002975"
     CDS             1290..2300
                     /locus_tag="ACG8M6_002975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0458546.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          835 a          366 c          471 g          704 t
ORIGIN      
        1 attttctcgt attttttcct tattcaaaag ttttgcagga aaattgctga aaaaataatt
       61 ttactaagta gaaaattcaa agccattcaa tcagtgattt ttttcagcac aagaaagact
      121 ccttataaaa atagcagata ctttgtcaat taagtgttta accagggatg ataaaaagga
      181 taggattgaa caaaacctgt ataaggtgat gaatagtttc ctcgccagta aaaggtttat
      241 tcgtataaag ctttatcgcc aaaatttatc atgacctttt ttatcttcaa tattaaatga
      301 aatcaattgt tcaatcaatt cccagttgat tacacttttc caaggaactt tgatgacgtt
      361 atccgtatat acgtagcctg cttcttggat atttttttca agggcacgaa tcgctgcttt
      421 ttccggtgcg atagaaaaat gatttttagc aaaactaaat gcaataataa aggtaccatg
      481 gtcagtaaac ataggttggt tccattttac agttgtgtct agctggggat acgtttctgt
      541 tatcttagca aaaaggtctt tgatcttttg ttgatgttct ttttctttga tttgattgat
      601 gatcggctga aatttttcca ttctaaaacg cctccattaa gtggtttgat ttattttacc
      661 agaaaaacaa tagttatgta taaaaaacga acaaaacaca agtaatgttg taaccgctat
      721 atttatatga taagatgtct caaataataa aatagaaaag agacataaaa atgattgact
      781 tggcaaagaa attcttacac gaaaatatat attttgagtt gtatcatcat cgagccattt
      841 atacaaatga agatgctttg gtaataaaga aagaacaagg atttacaggt acagagacca
      901 aaagcttatt cttaaaagat aaaaaagata ataactacgt atatctgact tttactacga
      961 aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa aaggctttct attgtttcat
     1021 cagaacgcat ggaagaacaa acgggacaaa agcagggcgc tgtttctccg tttggttatg
     1081 aaaaacagat tccgattatt attgatgaag agctcttatc aaatgaaaaa cttgtttttg
     1141 caccaggtag accagatcaa acgatggtca tccaaacggt tgacttaaac aaaatcatcc
     1201 agctacttga gatgccgata tttttgatgc cggcaaacga aaaaaacaat taaccaagta
     1261 aaatagtgca tacttggagg tgaagcaaca tgataagtta tccattatta gaaagaaatg
     1321 caacgattgg ggttactgca ccatcctcag gcgtatcaga ggaacaccat tcgttactcg
     1381 aatcagcttt aaagagaatg gaagaaaaat ctttccaagt agttactgga aagacgcttt
     1441 ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa
     1501 tgatgcaaga tgaaaatatc caaatgatct ttccgacttg gggaggagaa ttattgattg
     1561 agattctcga ttttgtcgat tttgatgctc tacaaccaaa atggatagta ggttattcag
     1621 atattagtac gttgctactt gctgtcacat taaaaacagg gatagccact gcacatggaa
     1681 ctaaccttat tgatttaaga ggagagtact tggatgaaac gacagcaatg tgggaaagtg
     1741 tactgaaaac aagagaagga gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag
     1801 tgtggcccca tgagcattca tcatcttctg ttttccatct cactgaacca acaaattggc
     1861 aatcagtcag cggagatgca gtaaggatga agggacgatt attaggtggt tgtattgata
     1921 cgatcaggca tttaatcggt actccttatg gagatgtcca atcttttaaa agaaagtata
     1981 atgatggaaa gccgatcttg tggtttctag aaaactgtga gctatcgtca gttgatttaa
     2041 gaagatcact tgttcagatg aaactggcag gctggtttga tggcagctcg gggatcatgt
     2101 tcggtagaag tgcggcagag gatttgttag attataaaat gaaggatgta tacgaagatc
     2161 tgtcaaagga attaaaacta ccgatcttgt atgatattga ttgtggtcat gtaccgccac
     2221 aactgacatt gattaatggt gcctatgcag aagtagcggt agaagataga aaagcgactg
     2281 ttttgcaaac gtttatttaa aaaatttttt cctgccttgg agtctatcaa agaccaataa
     2341 atctaaatag aaaagaaagt gaaagaagct gacaaa
//
LOCUS       DAZKOB010000202         2287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000202 DAZKOB010000000
VERSION     DAZKOB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.210"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..>2287
                     /gene="acm"
                     /locus_tag="ACG8M6_002976"
     CDS             172..>2287
                     /gene="acm"
                     /locus_tag="ACG8M6_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0458547.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          896 a          349 c          446 g          596 t
ORIGIN      
        1 accttgtaaa aacagttttt tggcatattt attttttata aatatttgta aaataactat
       61 caatttttta gttaaaagat tggaaatgag tgggggagga attgaagaaa ggatttttga
      121 gagatgatat agtaggtttt atttttagtt aaaatagggg agatagaaaa aatgaaaaag
      181 tgtagaaata ttattttatc aatgttattt attattacta atataacatc actgattcct
      241 gttcatgtct atgcggatgc aggcagagat atcagcagta atgtcacttc gttgaccgtg
      301 gacccaacga atattacaga tggaggaaac ataaaagtaa aattctcgtt tgacgaaaaa
      361 aaacaaaata tccaaccagg tgattatctt tggatcaatt ggccaagtga aggaaatatt
      421 cgcggtgaag gattccaaaa agaaatccca ttaatgattg aaaataaaaa tgttggaact
      481 ttaacggtca gaaaagattc tgcgcaggtt gtgtttaatg aaaatattaa aaacctagac
      541 tcagtagaag gttgggggca atttgagata caggccagaa acgtaaccga taccggtgaa
      601 gaaaatacgg gaccattcac cgtgacgagc ggtgataaaa cagctactgt aaatgtgaca
      661 aagccagctt ctggttcttc ttctagcgtt ttttattata aaacaggaga catgctacct
      721 gaagatacta agcacattcg atggtttttg aatattaaca ataacggcac ttatgttgaa
      781 caaccagtta aaatcagtga tgaaatccaa agtggacaaa gattagatcc aagtacgttt
      841 gaaattaatc aaatacactt aggtgaacag aaggtttata gaggagaaga gggaatccaa
      901 caatttttac aagatttccc cagcgctaca tttaattttt cagtaactga taattacatt
      961 gaaataacta taccaaaaaa ctttgtcaat cttaggaaaa taatggtgag ttataagacg
     1021 atcatagaaa acccagaaca aataaatttt gagaatcatt cggaagcttg gtttaaagaa
     1081 tttaataaac ctgctgttga tggagaaagt ttcaatcata cagttaaaaa tatttcagct
     1141 tctggtggag tcaacggtac agttagaggc gaattgaaga ttttcaaata tatcaatgac
     1201 acagagattg gtatcccaaa tgtgactttc gaattaagac gagcagatga acagcctatt
     1261 caaggacagt ctagtatcct gttgacttcg aatgaacagg gagaagctag tatcaaaggc
     1321 ctgcaagtag gagactatgt tgtaaaagaa aaagaagcac ccaattggat tgattttgat
     1381 ccactaagtt caaatgaact caaattctcc ataaacgaaa atgatacaga gggagtcagt
     1441 ttacctattt ataacaagaa aaaggttacg aacatcacag caactaaaat ttggaacggt
     1501 ggaaccactc caagaccgag tatttatttt aaattattca gagcaagtac aaacaattgg
     1561 gaaccagttc ctgatgcaga aacaaaacga ttagacaatg gaataacttc agtgacttgg
     1621 gaagatatcc aacagtatga tgatagtgga aacgaatata cgtttaaagt gcaagaagta
     1681 gatcaaaacg gaaatgatta tgtcccttca ggttatcaaa aaattgaaaa cgggctagtc
     1741 gttacaaatg agaataaaga agtcatttca gtctcaggac aaaaaacatg ggaagacaat
     1801 gagaaccaag acggcaaacg accgacgacg atcacagtaa atctattagc cgacggtcaa
     1861 ccgatccaac acaaagaagt aagtgaaaaa gatgattggc gctatcgttt cacaaatctg
     1921 ccaaaataca gagacgggca agaaatcatc tatactgtaa cggaagacaa cgtgcccgaa
     1981 tacagtacga cgattgacgg gtataacatc aaaaacagtt atacaccgga aaaaacaaat
     2041 atatctatac caggtagcca tataacaccg tggaaaaatt ttccagagaa gacagagaga
     2101 gagaaaatta gaaatctatt ctctcaaaaa ttgcaaatat ttgctcatac aggagaatcc
     2161 agcgaacaaa gaagcaatta tattagtaag agagctgtac ctaaacagat aacaaataaa
     2221 tcaaaaagca ttcttcctaa aacaagtagt aaaaaatcta gttttgctgt aatcattggt
     2281 ttattga
//
LOCUS       DAZKOB010000203         2232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000203 DAZKOB010000000
VERSION     DAZKOB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.211"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8M6_002977"
     CDS             <1..378
                     /locus_tag="ACG8M6_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0458548.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8M6_002978"
     CDS             426..1826
                     /locus_tag="ACG8M6_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0458549.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="ACG8M6_002979"
     CDS             1859..2026
                     /locus_tag="ACG8M6_002979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458550.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2232
                     /locus_tag="ACG8M6_002980"
     CDS             2080..>2232
                     /locus_tag="ACG8M6_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0458551.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          628 a          371 c          483 g          750 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttcgagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt
     2221 tatcgttggg tg
//
LOCUS       DAZKOB010000204         2183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000204 DAZKOB010000000
VERSION     DAZKOB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.212"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG8M6_002981"
     CDS             200..742
                     /locus_tag="ACG8M6_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458552.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="ACG8M6_002982"
     CDS             898..1347
                     /locus_tag="ACG8M6_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0458553.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>2183)
                     /locus_tag="ACG8M6_002983"
     CDS             complement(1377..>2183)
                     /locus_tag="ACG8M6_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0458554.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          765 a          356 c          419 g          643 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gtttggttat caaattcctt tccccggtct gtatgaaaaa gcttgacgtc tgttaaagaa
     1741 tacggtatcc gtccaaaggc ttcttttacc aatgaggcat ctttcttctc accacaggaa
     1801 taaccaataa tttctcgatt aaacaaatca agtattaagc agatataatg ccactttttc
     1861 cccacgcgaa cataagtaag atccgtaacg atggcttcca atggctgttc ttgtgtaaag
     1921 gtccgatcta atacattcgc tgttttcgct tcattacaag ctgttcgttg ccctttaaaa
     1981 tgagcgatcg tataggtaga tgtcaatccg cggcgtttca tgattcgacc gattcggcgc
     2041 cgactgagtt gaagcccacg ctttgctaaa cacttcttca attttcgggt accgtaagcc
     2101 tttcggtttc gaataaactc ttcctgaacg atctcttcta agtcggactc attttcgatc
     2161 ggtttcgctt gataatagta ggt
//
LOCUS       DAZKOB010000205         2160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000205 DAZKOB010000000
VERSION     DAZKOB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.213"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG8M6_002984"
     CDS             278..787
                     /locus_tag="ACG8M6_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0458555.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG8M6_002985"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG8M6_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0458556.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
BASE COUNT          754 a          375 c          417 g          614 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
//
LOCUS       DAZKOB010000206         2157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000206 DAZKOB010000000
VERSION     DAZKOB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.214"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG8M6_002986"
     CDS             complement(<1..914)
                     /locus_tag="ACG8M6_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0458557.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG8M6_002987"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG8M6_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0458558.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          732 a          381 c          416 g          628 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaa
//
LOCUS       DAZKOB010000207         2094 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000207 DAZKOB010000000
VERSION     DAZKOB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.215"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..418)
                     /locus_tag="ACG8M6_002988"
     CDS             complement(131..418)
                     /locus_tag="ACG8M6_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0458559.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(589..1941)
                     /locus_tag="ACG8M6_002989"
     CDS             complement(589..1941)
                     /locus_tag="ACG8M6_002989"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0458560.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT          576 a          418 c          359 g          741 t
ORIGIN      
        1 aaaaaaaggg gttgacaaag cttgtcataa cccctttttc tgattaaaag atagtaaaga
       61 gacagtcatc ggtaattgtg atgatttttt gtcatcactt aaggatgagc ggccttttca
      121 aaaactgtct ttaaagatca tcatcagttt ctagcacata tggctgacct tcatatgtgt
      181 actctgtatt caatgtgccg tcattttcat ctgagtagat gcgtctactg aaaggaaaac
      241 gatgagaaac attatctttg gcgtcatttg ctggcatcgt gtaatattcc tcttgcgttt
      301 cattcaactg agagagtact tgatctgcca actcatcaat attgatcgtt aatttcttcg
      361 aagcactttt ataatcttct aaaaatgcat cttgttggac aaaatattct ttattcatct
      421 tctttctcac ctttcttttc aaaatcaaat aaagctgctt gttcttttat gtaacagctt
      481 tatctttgct gcttattgtg acaaaaaatt cgaagaaaga caactaattg tacttttcac
      541 aaaaaaagac agagggtcgt gacaaaattg tgtcacaacc ctttttcttt attgtgcagt
      601 ttcgatttcg cctagcgctg cttgtgcaag taggttcaag taattccatg ggcggtcaaa
      661 atgtggttgg aagaagaaat ctgaaagtgc gagatcttct acagtcattt tattttgaac
      721 agctaaagac atggtatttg ctgattgtgt aatgtcgtat ttggacatga actgagcacc
      781 tacgatacga ttcgttcctt tttcatagac tagctccatc attacttttt cagtagttgg
      841 catgaactct ggacgataat tatcttcaaa tacagtcgct tggacttcta gatcgttcaa
      901 agttgcgctt tctttggtca cacctgtcga accgatcgtc cagccaaata ggtaaagtcc
      961 tgaagttcct tgagtaccac gatacgccat tttatgatct gtcaagttat ttcccacaag
     1021 caaaccttga cgaacggcat ttgttgccaa aggaatatag ttttgcgtgc cacttggatt
     1081 gtagttgact actgcagagt ctccagctgc aaaaatgtct ggattacttg tctgcatgta
     1141 ttcattcact ttgattgccc cgtttggcag tgtatctact tttccttcga tcagttttgt
     1201 atttggacgg aagcccacac ataaaatgac catatctgta tcaaatgtat ctgttggtgt
     1261 agctacttgt ttcacttttc cttcttcatc tgtgataaat tcagtaacat tttcgcctag
     1321 cgcaagagta acaccgcggt ctgtcaattc tttttctaag atatctgtga atggtttatc
     1381 tagatattta ttcaagatgc gatctaatcc atcaatcaat gtcacttgtt ttccagattc
     1441 gacaaaagct tctactaatt caataccgat atatccacca ccaacaatta ctacacgttt
     1501 tgcttctttc gcttgtgcaa taatttcatt tgcttgattg tagtttttgc ataggagtac
     1561 atttttcgca tcgattccac taatcggtgg gataataggc catgaaccag ttgtcatcac
     1621 taatttatca taagaaaccg tttctgtttc tcctgttttc agatcttttg ccgttactgt
     1681 tttattttct gtatcgacgt ctgttacatc gtgttccatt ttaactgttg ctcctaatga
     1741 agataattct tcaggatttg agtagaacaa gcctgctggg tcctttacta ctccgccgac
     1801 ataaagtgcg ataccgcaag ataagaaaga aatattgtca tttctttcat atactgtcac
     1861 ttctgcttct ggatgatttt ttaaaatact ttttacagct gcagtacctg catgagtaca
     1921 gccaattact actactttca tttatggtcc ctccgattaa cattttgtcc ttcgacaaaa
     1981 ctactataac agaaataaaa cggatacaac agtttaatgc ttgtgaattc acaaaggttt
     2041 gtttgtagtt tcattccttt tcaagcataa gaacccaata tctacaagat tctt
//
LOCUS       DAZKOB010000208         2083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000208 DAZKOB010000000
VERSION     DAZKOB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.216"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG8M6_002990"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG8M6_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0458561.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG8M6_002991"
     CDS             852..1040
                     /locus_tag="ACG8M6_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0458562.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG8M6_002992"
     CDS             1053..1610
                     /locus_tag="ACG8M6_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0458563.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG8M6_002993"
     CDS             1642..1884
                     /locus_tag="ACG8M6_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0458564.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          763 a          315 c          418 g          587 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     2041 tctataaaaa gaagaccgtt tattttttgt ctttatttgt gtg
//
LOCUS       DAZKOB010000209         2068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000209 DAZKOB010000000
VERSION     DAZKOB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.217"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG8M6_002994"
     CDS             <1..156
                     /locus_tag="ACG8M6_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFQ0458565.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="ACG8M6_002995"
     CDS             169..1077
                     /locus_tag="ACG8M6_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0458566.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1697
                     /locus_tag="ACG8M6_002996"
     CDS             1077..1697
                     /locus_tag="ACG8M6_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458567.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1701..>2068
                     /locus_tag="ACG8M6_002997"
     CDS             1701..>2068
                     /locus_tag="ACG8M6_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458568.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTT"
BASE COUNT          730 a          318 c          470 g          550 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaga atacagaggt tatggaagcg actatagttg aaaatggtct tccctacgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgtaggtg ggaaatatcc agtccaatta
      361 gaaacaaaaa ttgtggatgc taaaaaaggg aaaattaact atatttttga taaatattcc
      421 atgcagtgtt tacatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ttattttgtc attaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aatcgaagat ttaattgcgg atatgacgga ctttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgaacgttgg
      661 agacaagaac aggaacacag tttccaagat tggagagaag gacaagaatc agactatttg
      721 gcatggtttg aatcaatcaa ggatatttta aagacgattg atccaggtgg agtcatgtta
      781 gccgaactaa cggatgcgcg agtagatatt caaggagttc gtcatgcgtc tatttcagag
      841 cgtttgctgg cagatatgga ctatttatat cagaaattgc gagcaacgct tttcacgatt
      901 gaatacgggg agattgaagt gactgatatt ttgcaggatg atctcttttc agataatcat
      961 gaagtcgaaa aagttggaac tgtagcattc ccgattgaag aaggagcctt gatcatcgca
     1021 accgttgatg atccaaagca aaatgtgttc actcttgaga aagttggggt gatctaatgg
     1081 ctaaaactaa acgaatgatg gaaacggatg aaaaaacggg tgtacaacgc caattctttc
     1141 caatcacaca tgcttccgca gttcttgggt tagaagaaat aatagcagga gaagcaacag
     1201 ttttatctgt taatggaaaa attggagccg tcgtcattac taaagaagat ttgggattag
     1261 agaatgttct cacagaatta ccctatgcaa gtgaagaaga tgacggcatt atcacggcag
     1321 aaatgtatca aaaaatttta aacagtggag aaggtgacta cgtgttgcca gttgccacta
     1381 tcgaccgttt gggtggcata aaagttggtg aattattgac gattgatgag acagggaaag
     1441 tctctgcagt cagacaatct gatgtcaatt tttcgctgga gttaaaagaa aaactcgatt
     1501 cactgaaaaa ttatactgct ggagaaaaca tcactattga tgaagatgga acaattagtt
     1561 ctacaggtgg tagtgggact ggtggtgtca atcagtcata tgtagatcaa aaattccaag
     1621 aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt acttttgaaa
     1681 aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc aatgtacagg
     1741 tttatccaca aactcattgg aatgctatag aaggcaaacc aacaacggtg aagggagata
     1801 aaggcgatcc tggccaagca gcaacaatta ccataggaac cgtttctagt ggttcaaccg
     1861 cttctgttac gaatgtagga acatcatctg cagctagatt taattttgtt ttaccaaagg
     1921 gagataaagg tgatcctgga ataaatgcca caactacagc tgtagctaca actacagcaa
     1981 atgggttgat gtcgtccaca gataaaacta agttggatgg aattgcagct ggagcacaga
     2041 aaaatccagg taatgctaca actacgac
//
LOCUS       DAZKOB010000210         1986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000210 DAZKOB010000000
VERSION     DAZKOB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.218"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACG8M6_002998"
     CDS             <1..152
                     /locus_tag="ACG8M6_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0458569.1"
                     /translation="YRKLDGNLEHQDQVTLFLDFPFLYPGKNEIKWTKNIHSFEIMPR
                     WWRKV"
     gene            149..>1986
                     /locus_tag="ACG8M6_002999"
     CDS             149..>1986
                     /locus_tag="ACG8M6_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0458570.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          738 a          244 c          394 g          610 t
ORIGIN      
        1 cttatcgaaa attagatggt aatttagaac atcaggatca ggtcacctta tttttagatt
       61 ttccattttt atatcctgga aaaaatgaaa ttaaatggac gaaaaatatt cattcatttg
      121 aaataatgcc taggtggtgg agaaaagtat gaaaccaaga atatatagtc ctactgaaac
      181 agatttctca acgaatggct taggaatttt aaaggattgt acaagatgtg gaatatatga
      241 agtagctaat ggaaaatatg aattagagtt ggattatcct ttaggaacta gatttgatga
      301 atattttgaa aatgactatc aaataaaagc aaagccaaac gatcaagaag agtatcatat
      361 cttttttatt gatgataaag atatagatac ttttttaaat acagtaacta tttatgctca
      421 gagtcgtaca aatcgacttg gaagacgggt agtcactctt gtggaagtgg actctaaaac
      481 tggtcaagaa gcgatatcaa ttattgaaac taaaatggat aaaaaatctg acatacgact
      541 ttattctgat attacggctg tttctagcac aatctttgaa gcgagaaatg ttttaaattg
      601 tattgctggt gaacaaggat cattgcttca atattggggc ggagaaatta aacgtgaacc
      661 atttaagctt tctttgttaa agcgaagagg tcgcaataat gttggaacga ttcggtatgg
      721 aaaagatatg tctggtttga aggtcaaatt agattggaca ggtataaaaa caagaattat
      781 tccatatgct gatcctcaaa gtgaagtagg tacgactaat cgaatttatg gttcgccagt
      841 agatagtgcc tatattaata actatcctga tgtctataca gaacatgttc agtttacaga
      901 agaacaagga gtaaaagaca ttaagagctt aaataaaata gctaaaaatt actttaaaac
      961 gatcaatcct ggctgtgata agcctaaagt ttctattacg gttgaatttg ataagttgac
     1021 tgatagtgaa gaagcgaaag aatttgcgaa gattagaaat tatggtctgt ttgatacatt
     1081 taaaatatat cataaaaaat ataatattta tcttgaatca aaggttagtg gactacaata
     1141 cgattcttta tctgaaaaaa ctttgaaatt agaagcagga gacattcaag ttgcttttta
     1201 tcagcaacaa gctgtaacta ttcaggataa attaaaagat tatgcaacga ataattatat
     1261 gagtgatttt aatgattatg tttcttcaat gattacaggt caaggcagtg caggaggata
     1321 tgtagtttta tggccaaaag aaaaaccttc caacatcttt attatggata atccagattt
     1381 agaaaaggca agagaagtac taagaatgaa taaaaatggg attgcttttt ctaagaaagg
     1441 atggcggggg ccttttaatt cagcttggac attagatagt atatttaatg ccaattttat
     1501 acaaactgga ttaatcaaag cagatatctt tcaaaattct tttaacaaga ctggagatgt
     1561 attgaaatta gttaatggac tactccaaat ttggaacaac aaaaagaaaa taatggagtt
     1621 aactaaaaaa ggaatggaat tttggaattc taagggttca attggaacga ttggaacaac
     1681 tgattctgtt ggtaatcctt ttcctggggc ttctactcca acccctattg aagataattc
     1741 tttagttatt cgtacaaatg gagacggcaa atatattttg atttctccta aagtaggaaa
     1801 aggattagtt ttattaggaa atggtaaagc aatttatttt ggagacttag atgtacaagg
     1861 aaaacttaca gttaaaggaa aagaaataat tcctggacaa ggtggtggtc catcgggagg
     1921 tggagaatct actggtgggt atcctagtga attaaaaaca gatgcagaaa agagagcttg
     1981 gagaat
//
LOCUS       DAZKOB010000211         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000211 DAZKOB010000000
VERSION     DAZKOB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.219"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8M6_003000"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8M6_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8M6_003001"
     CDS             complement(728..1744)
                     /locus_tag="ACG8M6_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0458571.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKOB010000212         1955 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000212 DAZKOB010000000
VERSION     DAZKOB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.220"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..68)
                     /locus_tag="ACG8M6_003002"
     CDS             complement(<1..68)
                     /locus_tag="ACG8M6_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0458572.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYI"
     gene            423..779
                     /locus_tag="ACG8M6_003003"
     CDS             423..779
                     /locus_tag="ACG8M6_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0458573.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            789..1688
                     /locus_tag="ACG8M6_003004"
     CDS             789..1688
                     /locus_tag="ACG8M6_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0458574.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          654 a          322 c          384 g          595 t
ORIGIN      
        1 tcaatataag aaccctcgat attttgtgtt tcttcgataa aatagatgtt ttttatatat
       61 tccttcatag tgattcctcc aaagaagtct tttatttgat aagtttactc taccgaagag
      121 ttgttaataa tccgtgaaca aaaagtaatt ctttttccaa tagttgttaa aggtttttta
      181 ttttttatag tattttttgt tatttaagaa aggaataagg ttaaacaaat aacaggttgc
      241 tttttgggtc tttttccgtt caagaaactg aacgaagaaa agggaaagtt gtctcttttg
      301 tgataagaga aatcataata aacgaatgac ttactttcca ataaattgag aacagtcaga
      361 accagacaaa ttagttgcat ctgatgaata aagtgattat tctatgacat gaggtgatac
      421 agatgaaatt agaagtgaca aaagaagcac aagaagtatt gaagaataaa ttagaagcag
      481 acgatcaact tttattagat atcgaaaacg gtgacggtcc atttgcagaa agccaagtca
      541 gttgccaatt ggatacggca tttcgattga tcattgtcaa aaaagataca caaacagatc
      601 taagtcttta ttctgtagtg gctgatacac cagttggacc aatcaaaatc aaagacagtg
      661 caatacttta catggatgat ccatccacac ttgctttaga accaacttat aatagtttac
      721 aattaaaagg accaagcggc ttgagaaaag gcaacttgca agttgttcga aaagactaga
      781 gggataaaat gggaaaattg gcgatcatca gcgacctaca tgtggacatc aataaattcg
      841 gcgaatcaga gctgatgcag ctttggcaag ttttgcaaaa gcacaagatc actcggcttc
      901 atctagccgg agatacggct aataaagtag accgttgcct agctgttgtc gaatttttcg
      961 tcaataaagg attatctacg acatttcatt tcggcaatca cgagttggca gatgtgccca
     1021 atgaacagat gatggaccat tttccagacc cacatttttt aaatgaacgg tatattgctt
     1081 taaatgagca gaccgttctt ttagggatga atggctggta cgactatcag ttttccgaat
     1141 tgaaagatta cgaggcaatc gtacgaatga agcgtttgta ttggtatgat cggttcatcc
     1201 agcgtgggaa aacagatctc gaggtcaatg cagaaatgct tgcagctgca aaaaatttgc
     1261 tagaccagct ggaagcaaaa aaacttgagg tgattttatc cactcatttc gttcctaaac
     1321 gggcatttat catgtatcaa tctgctcctt atgagcgttg gaacaaatta aatgcatttt
     1381 taggatcaga atcattcgga gaattacttg accgttattc aaatatcaaa caagtagttt
     1441 ttggccatac gcatcgtcgc tttgaagata aatccatcgg tcacacgatt tacagctgca
     1501 gaccttttgg ttattattat gaatggcagc tgacccgaga ttttgtcttg aaagaacagt
     1561 tgctagacag ctatgatcct atgaaattac gtgtcctctt gcgtgcccat cacccagctt
     1621 ttcaggccta tcaaacacaa catttgcaag aggaatttga acaagcgatg acaatcgttc
     1681 cgtattgaga cagcattagt aatgtgtaaa agaaaatacc ttgtaaaaca taaggataag
     1741 taagagcagt agatccattt atcgtggata tgctgctttt tttgcggaaa ataatcagcg
     1801 aatgtttatt tgatcggatg atttgtaact cgcagagtac tagatgcaag cgtgtattct
     1861 ttttttgaac gataatcaca aaaaaaaata taatatacat tttttattta attataatta
     1921 atattgattg aagtctaggc agtcaagtcg agagt
//
LOCUS       DAZKOB010000213         1920 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000213 DAZKOB010000000
VERSION     DAZKOB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.221"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1149
                     /locus_tag="ACG8M6_003005"
                     /pseudo
     CDS             5..1149
                     /locus_tag="ACG8M6_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1320..1778
                     /locus_tag="ACG8M6_003006"
     CDS             1320..1778
                     /locus_tag="ACG8M6_003006"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0458575.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
BASE COUNT          629 a          361 c          378 g          552 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccaa aagatctagc cgatcagatc
      661 ggattttacc aaaatgctat cggagccgta cttggtcccc aagatagctg gttacttcag
      721 cgcggaatca agacactaag tgttcgtatg gaggaacatc aaaaaaatgc ttttgtggta
      781 gctgattttc tattttctca tccagcagta gaaaaggtct actatccagg attacccgat
      841 catgaactcc atggagttgc taagcagcag atgagcggct ttagcggaat gatttctttt
      901 actttaaaaa atgaagaatc tgctattcca tttgttgaat cactccaact atttacttta
      961 gcagaaagtt taggtggcgt agaaagttta gtggaaattc cttctgtcat gactcatgcg
     1021 tctattccaa aagaaaaacg agaagaggct ggcataaagg acggcttgat ccgtctttct
     1081 gtaggaattg agtatggtca ggatctcata aacgatttag ctcaggcatt tgacagaatc
     1141 aagaactaga agtcccaata aacgacttag cagaaaaatc taaaaaatat ttcagattgc
     1201 tacaccttgg aaacgataag tgttagaatc aagtagaaag acgtttttgt aaatttcact
     1261 aaataattct ttagtgaaaa gcatcaaaac gaatgaacaa acagatagga gtcttacaaa
     1321 tgagaatgaa tgtcgaaagt tttaatcttg accatacaaa agtcaaagca ccttatgtac
     1381 gtgtagctga tcgaaaaacg ggtgcgcatg gcgatgtcat cattaaatac gacgtccgct
     1441 ttaaacaacc aaataaagaa cacatggaca tgccaagtct acattcactt gagcacttga
     1501 ctgccgaact aatccgcaat cacgcagatt acatcgtaga ctggtctcca atgggatgcc
     1561 aaacaggctt ttacttgact gtattgaatc atgaaagcta cgaagacatt ttagatgttc
     1621 ttgaagcaac aatgaaagat gttcttgaag ctactgaagt acctgcaagc aatgaaacac
     1681 aatgtggctg ggctgccagc catacactag aaggtgcaca agctttggct cgtgaatttt
     1741 tagcaaaacg cgatgaatgg tctgaagtag aagcatagtt tctaaaagaa cgtaaagatg
     1801 agaagcaata tagctatctt atcttcgtat tttattaaat atttattgtc tgaaaagagt
     1861 tctaaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
//
LOCUS       DAZKOB010000214         1895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000214 DAZKOB010000000
VERSION     DAZKOB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.222"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..>1895
                     /locus_tag="ACG8M6_003007"
     CDS             134..>1895
                     /locus_tag="ACG8M6_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0458576.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLV"
BASE COUNT          739 a          259 c          333 g          564 t
ORIGIN      
        1 aatattgcag gcttttttat tgatttgaaa taatcaagaa tattagaaac ttctacatta
       61 atcaagaact ttcttctgta tgaaaataga gataataatt gtacacttaa gctgtattca
      121 ggaggtgaag ataatgggca tcgaatttag cgaacggcaa atgaaaatac tatacttttt
      181 gattgaaaat gagcacacaa cttacaaaga attatcagaa caattatatg tatctacgaa
      241 aacaattagc aaagatattt taattttgaa agaaataatc caaaaaatgc cagatgtaca
      301 aatgttggtt caacctaaaa aaggcgtttc gcttattgga aaaaaagaaa cacttcgaaa
      361 tttattatcc agtcataaaa tcaatatcgc agactctccg gaggaccgtc taacttatgt
      421 gtttgctaaa ttattgaata ctaatgattc tctaaagatt caaaatcttg ctgacgaatt
      481 atacgtgagc aggtcgacgg tagaaagcac aatgaaagat gttagaaaaa gactaaacaa
      541 gcatgggatg tccttagttt cagatcgaaa tggcgtgcgc atcaaagctg atgaaaaaag
      601 taaaagaagg ttgatgtcag aagttgtgaa ctattattgg ggtggcttta cagcatctaa
      661 cagtaaaaca gatctcttag acttgaatat caatttcatt ggtgaagcga ataatttctt
      721 aaataaggac atattgaaaa aagtagccga attgcttaat cgatacatga ataagacaca
      781 attgaaattt acagattatg aatttcaaac tttagctatc caccttgcta ttgcgataga
      841 aaggattgaa acaagcaatt tcttatcacg tagtaataat tctgaagaag aaatctcaaa
      901 gaatacactt attttaatca aaatgattga aaaagcattt tcaattattg tgccaataga
      961 tgaacaagct tatgttgaca ttcacattac ttcgattgaa aaaaatgcta tgaatcacga
     1021 cttagatcaa acgaagcttt cctatttaga tatgggtgtt gagctacaag agttagtaag
     1081 aattaaatta aatgaatttt atcctgatga agaattgatc aataatctag tattacatct
     1141 caatgcagca gttaaaagac tcaaattggg agtaaatatc tataaccctt atacggacaa
     1201 aatcaaatat tcttttaaaa ggtcatttat gataagtgta gatttattag aggaaatcga
     1261 agagagattc tgtatacatt ttaatgaaga tgaaatcgct tacgtaacac tccatattca
     1321 atcacttttg gatcgttaca aacctgacaa aacgaaagtg atattagttt gtagtagcgg
     1381 atatggaaca tcaaaattat tagagcaaag aataaataac ggttttgcag atatggttga
     1441 gatagaagat gtactaagca tcaatgaact acaagataga aaagtcactg atgaacttgt
     1501 tataagcacg attccgattg aaaatacaaa ttttcctgtt attgttgtta gtcctttgat
     1561 gttaggggca gatgtcagaa aagtaaaaca gtatctacag ttaaatgaac aaacagaaga
     1621 tgcttttcta aatttattag ataaacgatt ttgctttttt acccaccaaa taaaggataa
     1681 tcaagtgaac gttttaaata taattaccaa aaaattggta aaagaaaatt acgcgaaaga
     1741 agggatacta gagggagcaa tcaaaagaga aaaactatct tcaacagcga tgaatgattt
     1801 tgctatgccg catatcgaag tagattttat aaaaaagcca gtaatctgtg tctacgttaa
     1861 tccagaagga attgattggg atgggacact tgttc
//
LOCUS       DAZKOB010000215         1887 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000215 DAZKOB010000000
VERSION     DAZKOB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.223"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1131
                     /locus_tag="ACG8M6_003008"
     CDS             <1..1131
                     /locus_tag="ACG8M6_003008"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0458577.1"
                     /translation="FLMIVVVLFSLSAFSTGTILGWQFYNNEHSQIVAHAQSAQKKLI
                     LTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKLTQQPNDPSLYYQI
                     GSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDLLYMRSGLHRTASP
                     TIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQPYEKLLTDDIIEP
                     LGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNELGTGNISMSVYDLN
                     TLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQGNIDSFDTSAIFSK
                     DSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1398..>1887)
                     /locus_tag="ACG8M6_003009"
     CDS             complement(1398..>1887)
                     /locus_tag="ACG8M6_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458578.1"
                     /translation="ILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKK
                     DQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKN
                     ATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTS
                     HE"
BASE COUNT          652 a          327 c          289 g          619 t
ORIGIN      
        1 tttttaatga tcgttgtcgt gttgtttagc cttagtgcgt tctctacagg aactattctt
       61 ggatggcaat tttacaataa tgaacactct cagatagttg cccatgctca atcagctcaa
      121 aaaaagttga tactgactac caatgaacaa cagctcgttg aaaaaatcga tcaactatta
      181 agaaaaaatg attatattgg ctcaatttac gtaaagaaaa ataatcgcat catcatggaa
      241 aaaggctatg gctatgcgaa taaactcacg caacaaccaa atgatccttc tctttattat
      301 cagattggtt cggttcaaaa agcattgact actcttttga ttttaaaaca agtcgagcgt
      361 gggcaccttt ctttagatac taagctggct caattctatc cacaaattgc tggcagccag
      421 caaattacta tccgtgactt gctgtatatg cgctcaggct tgcaccgaac tgcttctcca
      481 accataccga tgactgatga agaagtcgtt caatttgcga ttcagcactt aaaatttaca
      541 gattatcaga cctctcatta tgattcattg aactttacca tattgactgg aattttgatc
      601 aaattaacaa atcaaccgta tgaaaaatta ttgaccgatg atatcatcga acctttagga
      661 ttacagcata caaaatttta tgaccaagtc aaaaattcat ccaatcatgc actatcctat
      721 caaatgtcta aagaggatga ttactttaaa ccactgacag aatctgaaac agagatccat
      781 aacgaattag ggactggtaa tatcagtatg tccgtctatg atttaaatac gctgtttaca
      841 caagttttat ctggaaaact agtttccaaa gacttactct tctcattatg gcgtgaaaat
      901 gcgaatggac atccatatag tggtggggta tatagcggag ataattatat cctgtcgcaa
      961 ggaaatatcg actcttttga tacatctgct atatttagca aagattcaca ggatgctatc
     1021 atcatggaaa gcaatataaa agctgacaaa actatcaaac tgcctactgc tgatctaaga
     1081 aaccaaatct atgacctgat ggaaggaact actaagttgg caaatagctg atatgatgaa
     1141 aagatagcaa atactacttc tttgaatagc atttgcttaa aaaaagtact cgactaaacg
     1201 taggaaagag tgtgggacaa atctaaaaat caatttttgc tacagacttt aattccaaat
     1261 aaacagaaca gaaagaggtg ttcttctttc tgttctgtcc aaattattct aagatttctc
     1321 tttaccaaca ttatttgaca aagggcctat taaatctaaa atagtatctt tagattgcta
     1381 aaatatgtgc tagcaaatca ttcatggctt gttcttattt ttaattttat gcaataaaaa
     1441 agaagaacaa ttagcgtgat tatagaaata aataatgaga ttttagaaaa aaatgtgtat
     1501 gaatacctga ttgttacttt atttattcca ctttcacttt ttactgtaac aaaaggacct
     1561 ccttgtttta gtactgtagc attttttgaa ttaacagtaa ccgttaatcc tttataccgg
     1621 aatacaggta aaactattgt tttgcctttt gaagatttaa acgtaaatgt aatttcatct
     1681 ttatttggag aaaagctact ataaataact tgaccatctt ctgtaacttg atggtttttc
     1741 aaaagcaatt gatctttttt atagcctata ggtacataat caaaattctc aaattcttca
     1801 agtttatttt ccatttgttc attagaataa gttccttgaa aattcatttc tttcaccatg
     1861 ttaactactg atgaataatt taaaatt
//
LOCUS       DAZKOB010000216         1781 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000216 DAZKOB010000000
VERSION     DAZKOB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.224"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..803
                     /locus_tag="ACG8M6_003010"
     CDS             207..803
                     /locus_tag="ACG8M6_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0458579.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(861..1343)
                     /locus_tag="ACG8M6_003011"
     CDS             complement(861..1343)
                     /locus_tag="ACG8M6_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458580.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1449..1646)
                     /locus_tag="ACG8M6_003012"
     CDS             complement(1449..1646)
                     /locus_tag="ACG8M6_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458581.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          602 a          362 c          270 g          547 t
ORIGIN      
        1 caaataagca aaaattccta aaacacaaca atgaaagcga taactaagaa aatccttaca
       61 taacgatttt tcttattaaa attgtttttt gaacttcgct tattttttgt gagcatatct
      121 gtttacttac tttttgtaag gtgctatact tcatttcgta atcaattaaa aaaatgcaaa
      181 acgcaaatca aaggagaaat tttattatga caacttttac tgacactttg aaaaaccgcc
      241 gttcaatcta tcatctagga cgtaatgttt cactttctaa tgaagaatta acaactttga
      301 tcaaagaagc aattaaagaa agcccaactg ctttcaatgc tcaatcaaca cgcgcagtta
      361 tccttttcgg agatgcacat gaaaaacttt gggaaatcac tgaagaagca cttcgcccat
      421 tgactccagc agaagctttc ccaaatacac aaaacaaatt agctggtttc aaaaatggtt
      481 atggtacagt attattcttc aaagatactg acgtggtaaa aggtctacaa gaacaatttg
      541 aattatatgc agacaacttc ccagattggt cagaacaatc aaacggaatc gcaacagcta
      601 atacttgggt agcattagtt gataaaggat tgggcgcaaa cttgcaacat tacaacccag
      661 ttattgacga agcagttgca aaagaatgga atatcccttc aaactggaaa ctacgttcgc
      721 aattagtatt cggttcacca gaaacaccgg ctgaggaaaa agaatacatg aacgatgccg
      781 atcgcttccg cgtatttgga taattttatt tgaaataaaa aacagaggtt gtgacaaaca
      841 tcttgtcgca acctctgttt ttattctgga tcaatcataa tatcaatctc tgcctcatcg
      901 tttaagcgtt gggacaaaaa tggaactaat tcttctaatg atgcagaaaa tttcagcatc
      961 ggtcctcgcc atccgtttcg gaaaaaattc atttttacga ttttagaacc ttttggctga
     1021 tataatacaa ttttattgat ctcagaaaaa ggaacacctt ttttgtcaaa gctattcaaa
     1081 acaagtcctt cttctgtgat tcctctactg tccaacaaaa aactaataaa aatcattgct
     1141 gacaagatcc cttttgccaa ttcatctgct gtaggatgac tgatgagtgt ataccaaatg
     1201 actagtgaca ctaagaaggg tatcaccaag tagacccatc gatttcgcac agtccgataa
     1261 ataatttctt ttcgaaccag ccaaagaaat aaaaacatca caacaacgaa taaaaacaat
     1321 agaaccgtaa agaatgttac catgacaact cgcctcgctt tttcttctcc agaaaatagg
     1381 aaaagaatac tgatttacct tctcaaagaa attggacttg cagcacgccg cacgtccaat
     1441 tgttttcttt attcaaatgt atcatatttt gcaacaacca tgcttgcttt ttcatctaat
     1501 aattcttggt cctctgcagc acattgggca actttgacca cggcgcagtt tgtcattttt
     1561 gatacaactt caccaatgac cgtttttgta aaaccaattg gtttgcatgc atattgttct
     1621 cctaaattaa tcatttcttc cgtcatcaaa atcctcctaa actgctggaa cttttctcca
     1681 cattaccact gctatttctt tggagattta actcattgat ttttatttac agttaaaaat
     1741 caaccttaca ggcattatag cgtactgttt tcgttttcac a
//
LOCUS       DAZKOB010000217         1728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000217 DAZKOB010000000
VERSION     DAZKOB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.225"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG8M6_003013"
     CDS             <1..156
                     /locus_tag="ACG8M6_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFQ0458582.1"
                     /translation="GTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAIS
                     SIVIPPK"
     gene            169..1077
                     /locus_tag="ACG8M6_003014"
     CDS             169..1077
                     /locus_tag="ACG8M6_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0458583.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1077..1694
                     /locus_tag="ACG8M6_003015"
     CDS             1077..1694
                     /locus_tag="ACG8M6_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458584.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          629 a          231 c          368 g          500 t
ORIGIN      
        1 ggaacttatg gacatactgg cgttatccga ggcttagcta atggtcgtat gcaaacttat
       61 gaacaaaaca cagagttagg tatgatttgt ggaaagttag atcgccagta ttatagtgca
      121 agtgcaattt cttccattgt cataccacca aaatagggag gtgatttaat ggcaaagtgg
      181 aatgtcatat taagtacaac agaaccctat aattacgtgg ggatgattca agttcgacaa
      241 ggcaataaaa acactgaagt tatggaagct acaattgtcg aaaatggtct tccatatgat
      301 ttatcagaat gtaaggtata ttttgaatca gttgttggcg gaaaatatcc agtccaatta
      361 gaaacaagaa ttgtggatgc taaaaaaggg aaaatcaaat atatttttga taaatattcc
      421 atgcagtgtt tgcatcgaca aacagccaat ttcattatat ttaaaggaga agacttgatt
      481 ggaacaactc aagacttctc ctattttgtc atcaatgctg tttcaaaaac agaaggagaa
      541 atgggttctt attggcaatc aattgaagat ttaattgctg atatgacaga ttttattaat
      601 gaaaataaag gcgattttac ggactggatg aatgaaagga aagaagaatt tgatcgctgg
      661 agagaagaac aggagaatag tttccaagat tggagagaag gacaagaatc agattatttg
      721 tcatggtttg aatcaatcaa ggatattttg aagtctatcg atccaggtgg agtaatgtta
      781 gccgaattaa tggatgcacg tgtagatctt caaggtgtac gccatgaatc gatttctgaa
      841 cgtttcctat ccgatctaaa ttatttgtat caaaaaatga aagcagcact tttcacgatt
      901 gaatacggtg aaattgaagt gactgatatt cttcaagacg atctcttttc agataatcac
      961 gaagtcgaaa aaattgaaac agtagaattt ccaattgaag aaggagcttt gattattgca
     1021 actgttgatg atccaaaaca gaatgttttc actcttgaga aagttggggt gatttgatgg
     1081 ttaaagtaaa gagaatgatg gagactgatg aatctggtgt taaacgtcaa tttatgccaa
     1141 ttacacatgt ttctgcagtt ttaggcttag aaaaaatgat atcaggacaa tcaaaagttc
     1201 tttcggttaa tggtaaatat ggtgcagtca ttttaacgaa agctgattta ggattagaga
     1261 atgcaattac tgaactacct tatgcaagcg agacaagcga tggtattctt actgctgaaa
     1321 tgtttcaaaa gatcgtaaat ggagaaggtg gaacatatat tttgccgatt gctactcctg
     1381 aacaattagg aggtataaaa gttggggaac tcttagaagt tactgaagaa ggtgttttat
     1441 ctgcaactaa gcaaacagac ttcaatttct ctgaagaatt aaagggaaaa ttagaatctc
     1501 taaaaatatt aaaagcaggt gcaaatatat caattgcaga agatggaaca attagttcta
     1561 caggtggtag tgggactggt ggtgttaatc agtcatatgt agatcaaaaa ttccaagaag
     1621 ctgtaaatca agcagaaaat tacacaaacg aaagaattcc aaattttact tttgaaaaaa
     1681 tcggggaggt atagaaaatg acagatatcg taaaaataaa acaaagcg
//
LOCUS       DAZKOB010000218         1698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000218 DAZKOB010000000
VERSION     DAZKOB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.226"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1018..1359)
                     /locus_tag="ACG8M6_003016"
     CDS             complement(1018..1359)
                     /locus_tag="ACG8M6_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0458585.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1359..1589)
                     /locus_tag="ACG8M6_003017"
     CDS             complement(1359..1589)
                     /locus_tag="ACG8M6_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0458586.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          564 a          278 c          236 g          620 t
ORIGIN      
        1 ataaccaaca gcaaaaaata caatgaaaaa caaacttgga actaatgtat cattaataaa
       61 tgttgaaaaa ttcagcaact cattactgta tgattttagc agcccaacca acatataccc
      121 tataaaagct aataagatgg ttaggaatcc tttagaattt ttactcattc atagacactt
      181 ccttaaataa cttttattat agaaggtatt gtaaataata atattgacaa aaagtgactc
      241 aaaataaaaa aaaccttaca ttatactgga atgaactgta ttgatagtgt acctaaaagc
      301 tctactgaca taagatcgtg taatcggtaa agtaatttta ttaattatac gggatgtaat
      361 caattagaga ataaactaat cataaaaggt acttttacat tcataattgt cgctttgaga
      421 ttctactttg gttcactatc tgacccaatt gcacattttc gatcattaca agtattggtg
      481 tcgttagaac aactaaataa aaactagcgg tagatttttc agaatctatg ctaatttctg
      541 tatggatgtt ccagtgatgg caaatttctc ttttcattgt ttttcttgtc gcatgatatg
      601 cctttttgtg ttattcagtt aaactataga aattctttcc tagacagatc ttgatcccaa
      661 attttacgtt tttgagcatc gatatagttt aaaatgacat tactcatatc taaagagttt
      721 agatttttta ggactaaaac tgtcagttgt tctttttctt ctttctgtta aagtattgtc
      781 gcgacttttc gtatacgtta agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa
      841 cgaatgactt gaggaaatca cctcaatcat tcgtttaaaa tgctatatat tctttagttt
      901 aagatacgga aattaatgcc atcgaccaca ctagtagaat gaacgacatt tacttggcct
      961 ttttttaatt tcgttatgtt tagcatataa aaatattata gagtttactt acaatgctta
     1021 aaacatatac tgtataatct gatcaatttt agccatgtct tgcaatggta agttttctat
     1081 ttttttgagt tttcgttcct taaaatcaag agattttaat tgtgtaataa ttacttgtcc
     1141 ttttatatct agctcttcag gtaatgagta acgtgtcggt aaattcttta tagtggaagt
     1201 aattggacat atgaccgaaa acattgtttt acggttaaat tcataacgag agacgactag
     1261 tcctggtcgt cttttttgaa tttcttttcc tacggaggga tcaaaatcaa tccatacaat
     1321 atcacctttt ttaggaatat aaattctttc ttctgacact ataaaatttc tctcccttca
     1381 ggagttaaat cttcccattc gtctttctca taatactcgg cctcttcccc accactaaat
     1441 ggatcttcaa tttttggaac taacgtaatt gttccatcat cagaatacac aacgatatat
     1501 tcttggtttt ctgaaggttt ctttccgtta tcagaaggta aagttaccac tacagaactt
     1561 ccttgtaatc tagattttgt aacaagcatt atcatcactc ccttaataaa agtatactat
     1621 ataacactaa gtaacgcaaa attccccttt gtaaactatc tagaaaattg ttttgtttaa
     1681 agtatatctt attggata
//
LOCUS       DAZKOB010000219         1676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000219 DAZKOB010000000
VERSION     DAZKOB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.227"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..403
                     /locus_tag="ACG8M6_003018"
     CDS             5..403
                     /locus_tag="ACG8M6_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0458587.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="ACG8M6_003019"
     CDS             387..1082
                     /locus_tag="ACG8M6_003019"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0458588.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          630 a          246 c          329 g          471 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtt
//
LOCUS       DAZKOB010000220         1664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000220 DAZKOB010000000
VERSION     DAZKOB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.228"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8M6_003020"
     CDS             131..505
                     /locus_tag="ACG8M6_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0458589.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8M6_003021"
     CDS             complement(619..1248)
                     /locus_tag="ACG8M6_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0458590.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8M6_003022"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8M6_003022"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZKOB010000221         1603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000221 DAZKOB010000000
VERSION     DAZKOB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.229"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..1554
                     /locus_tag="ACG8M6_003023"
     CDS             88..1554
                     /locus_tag="ACG8M6_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0458591.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          445 a          292 c          333 g          533 t
ORIGIN      
        1 tatatttctt aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg
       61 aaaatttaaa gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg
      121 cagccgaagg gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac
      181 ggtatgcgtg cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga
      241 ttaccacgag caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct
      301 agtatcgtcg gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc
      361 ggtggagttt tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca
      421 ttgtttgttt cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct
      481 ggcatggtcg gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt
      541 ttttacatgg gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt
      601 caagaatata actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta
      661 ttacaataca tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat
      721 cctttgacaa ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt
      781 atcgctgcag tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc
      841 aatacaatca gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact
      901 tcaaaagatg taactgctga agaaaaacca agagtatggg cttatatccc attattcctt
      961 gcagcaatca ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct
     1021 agtgaacgta cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca
     1081 ttgaatccaa ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt
     1141 ggtgaacgtc aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta
     1201 tcgtacttgc tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca
     1261 ttgtggttgt tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt
     1321 ggtttgtctg tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt
     1381 tggttcttag ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca
     1441 ccgggaacag aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc
     1501 ttgctattta ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg
     1561 aacataaaaa caggaattgc ttatttgcag cagttcctgt ttt
//
LOCUS       DAZKOB010000222         1554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000222 DAZKOB010000000
VERSION     DAZKOB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.230"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..>1554
                     /locus_tag="ACG8M6_003024"
     CDS             146..>1554
                     /locus_tag="ACG8M6_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458592.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTP"
BASE COUNT          570 a          215 c          281 g          488 t
ORIGIN      
        1 gcaatattct caaaaaaaag ataaaaaatg agaataactg atgaaaaaac aagcataatc
       61 catcataaaa tcagtatagg tgaaaagatg aactttcatc tgcatatcaa gtaattggta
      121 tgaggcattt tttgaggtgt aggatatgaa gttggattac cttatagaag aggaagatag
      181 agaaaaactg ctgctagcta gatatttaga atttgagaaa aagaattatg tagtgctgcc
      241 taagttatat gaaacattag gtttgtctaa atttaaaaca agaaattata tcatagaatt
      301 aaataaagaa tttactgaat tcaaaacaaa gccagagatc caaatttcag aagaagcaga
      361 gatccatgtc gaacgtctga atttgacgga tatcaaaaat ttaagaagaa tctactttca
      421 acgatcaaaa attgctcgac tgttaacgga gatcgtggat tcaaaagtaa cgatcgaaaa
      481 atttgcagag aatcattttt taagcatatc aagagcctat gtaaaaagaa gagaattagt
      541 ctcttttctt gaacagcatc aaataaagtt aaagaaaaac atattagttg gggaagaaat
      601 aaacatacgg aacgtattat ttggggtata tttcggggtc tttaatggtt tttctatgcc
      661 atttagagaa agcgtcatga atgaagcaaa gagtatcgtg caatattttg aatatctgtt
      721 caatttaaaa ctgtctgcaa caaaacgaat caaattcaat ttgctaacca ctattagttt
      781 gttgagggcc tatgggaatc aaccaatcgg aaaacgtttc ttttcagatg aatactttaa
      841 gagctcagaa ggaatgaaga tcatcaagga tttatcacat atttcttttc ttcatatccc
      901 taaagagcag atcattgatg agatttctta tatcatgttg tttttgatca cggaagaaga
      961 atttgaatta gatggcgcat ttggacttgc ggaagctcat tttcatgata tccacatgat
     1021 tagtcaaaaa atgtcagtgg aattgattga tcaattacca ttttcgattg aaatctctga
     1081 agaagaaaga ctatatatac gaaaagaact tcgtatgggt atgatgcatg tcaatcgaaa
     1141 aaattggata ttcgactttg agatgactag ctttacgtct aaaaagcagc tacagttttt
     1201 cttcgagacg tatccaatat tcagtacagc aatccaaaag gtcatacaag agaataaacg
     1261 ctatttcttg ggaaaagatg aacgtttcat gattcgctta ttttatgatt atctcttttt
     1321 attggtcggt atttgtccgg tatcatattt tgaagcaccg attcatgtct gtattgattt
     1381 ttcgcaagga tctgcttata ccgattacat tatttctcaa gtgaaaggat ttaaaaatta
     1441 caatattgtg attgagagtc gaatcacttc tcgtacgaat atttatttat ctgattgttt
     1501 acttgaaaaa tttaataaag aacagataat ttggaaaaat ccaccgactc caga
//
LOCUS       DAZKOB010000223         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000223 DAZKOB010000000
VERSION     DAZKOB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.231"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1536)
                     /locus_tag="ACG8M6_003025"
     rRNA            complement(<1..>1536)
                     /locus_tag="ACG8M6_003025"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAZKOB010000224         1522 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000224 DAZKOB010000000
VERSION     DAZKOB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.233"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(224..1243)
                     /locus_tag="ACG8M6_003026"
     CDS             complement(224..1243)
                     /locus_tag="ACG8M6_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0458593.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1240..1464)
                     /locus_tag="ACG8M6_003027"
     CDS             complement(1240..1464)
                     /locus_tag="ACG8M6_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0458594.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT          422 a          318 c          276 g          506 t
ORIGIN      
        1 gagccgtctg ctctgaccaa ctgagctaag ggtactggtt gttgccacat aaagccataa
       61 acaatcaacc agtagaatgt gtggcaacaa acctgttatc gcatagcttg gagtgtgact
      121 attatgggtg atagtgaaga tatgcgataa catcactatt ttatcgaatg atttttatag
      181 ttgtcaatat agttatgtaa tgctcctcaa cgaggagcta tttttatcgt ttaggaatat
      241 ttaaatacca acgtttgtca tggaaatctt gcgcaccgcc tttagtgttt ccctctggat
      301 cattcgttgc ccgcatcatg acatagactt tcttattagg gaagttacgc atgttaaaag
      361 atacatgata gccaacattt ccagaagtat tataagcttg atttacatct gatctataaa
      421 ttccatcagc tcttactcga gctaattctt tcccagtatt gtaatccata atgaagatat
      481 actcgtattt atagttagca atgtgccatc cagctacatg caaatttgca ttttcgattt
      541 ctccaaactg atcaatgtgg gcgtaattcg ttccatctgt cagtgtagga ttagctgcac
      601 cagctcgtgt tggatcaatg acaggcttgt tttcagaagt tgttggattt tcatcggtaa
      661 atccatgagc taaatcatat gctaattttt ctttacttac gcccatttca gaaagataac
      721 cgtaaggatc tgtatgatcg ccccagatgt tttgtgttac ccataaatgc gatttgattc
      781 ccggttggtt ataaggagtg tccaacgtta atggaatacc atatttcatt gctgaatctc
      841 tagccaattc aacgtatgcc ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt
      901 gtaactcaat ctgcacagga ctgttggcat tagcatacga accagcaccg tactgtacat
      961 aaccaggttg accgacttga taaacaattc cgccgtctcc cacaatataa gcagtataag
     1021 cgctagtcca tgaacgttgc atatactgcg cttcattgcg tcctgttgct gtttcattag
     1081 ccgtttcatg cagtaaaata tactgattat ttgctacttg tgagctacct tcatttgcgc
     1141 ccaaattaaa ttcattgttg atagtatagg caaatccgtt aattggcaat aaaaaaagag
     1201 ccgttaacag gctcatcgca gtaatagtaa ttttcttttt catttgtttc ctccttcttc
     1261 gctttcagcc gagaacattt tgtaggttcg atttgataca cccaacacac tccctaaaaa
     1321 cgcgccaaaa ccagtaatga tgacaacaca gatatctgtg tactgccaat tgagcgcttt
     1381 accaactaac cccacgaaag tagctagtgc gggaataatt accagtgcga accattttag
     1441 tacttcgaac gttttattat tcattttctt ctctccctaa acaaagtttt gatttgttgc
     1501 gtgtgttcca ccaatttttc tg
//
LOCUS       DAZKOB010000225         1502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000225 DAZKOB010000000
VERSION     DAZKOB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.234"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..220)
                     /locus_tag="ACG8M6_003028"
     CDS             complement(<1..220)
                     /locus_tag="ACG8M6_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0458595.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFK"
     gene            complement(373..1284)
                     /locus_tag="ACG8M6_003029"
     CDS             complement(373..1284)
                     /locus_tag="ACG8M6_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0458596.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          462 a          293 c          210 g          537 t
ORIGIN      
        1 ctttaaaatt gacattcaaa agcaagccaa atggaataaa gattataaca ttatctatca
       61 tctctctgaa attcccagta gcagtaaatg gagtcaagtt aagacttctt tgatgataat
      121 taaatactga taaaatattg tattgtaatt tgaataacac taaccagatt agtgtcacta
      181 aatataaagc tagcaatcct ctagataata tttttcccaa tataatttcc tctaattctt
      241 ttgtttaagt tgtatcatta gtatagcaat gtcctagtta acgtgggttt taaattaaaa
      301 agctaacgtt accccaaaag tgctagtaca ataatgctgt tcattaattt cgttggtttc
      361 attccgccat ccttacctcc ttgaattagt atgtgttgat ccgggaaaca ctgtcacaat
      421 tacaccgtct atattgtttc ctgatatctc ataacgaaga ttagtcggca catgaatggt
      481 ggtattttta gtaacaggat aataaaagat ctcatatttt tccccattta cactaacaga
      541 aatggttttt tcttctttta ggtaatccat atattcctcg agaacgaaat tcttttcttt
      601 cataatcgca ctatgtggta aaccaacata gcgaatatgc catggttcat attgaattcc
      661 tgttaactct gttttgtcct ctggataacg taaaatgaac ccgtatttcc aagcattttc
      721 ttctatccac tttccttcag gggctcgttc cattttcgtc aagcttgatc ctacatctag
      781 tgataaacct gaattatgct cactataacc tgctggtaag gcatactcag cccccatttc
      841 ttggtaaagc acactttgct catcaaagtc tcgatagcca ctattaataa taaaatgact
      901 aacgccaccc tttacagcat cattgaccat ctctgaaaat ttttgtgcta tttcttttga
      961 catataaata ttactatcaa gcaacccgta tccatttatt aattcgtcat gtttagataa
     1021 attcacgata tctgacttca cactttcttg gcgaacagga tatttactat tgattaatag
     1081 cagatttcct tgataaacct gttcttctgt aattgttttc tcttgggtat tttcagaagt
     1141 cccactattt tctaaatgtt ctttgggatt ttgatcataa ttttgaaatt cgactttttc
     1201 ctgagaaaac agtgcttcat taacgtagtc ataacctaag tatattaaga ataacaataa
     1261 caataaaaaa aacaacttct tcattttcag tctcctcctt aactatatag ttggaagtat
     1321 gaaatatttc tagaatcttc cccttaaacg gcacaatctc gtttgagatt gtgccgtttt
     1381 gccccgtaaa ttacagtcaa ttatttcgcc ctactggatt tactattatc accaatgtag
     1441 atgaattttc ataagtgata cccatgctga atttaacgag gctgaactgc ctgttgacaa
     1501 tg
//
LOCUS       DAZKOB010000226         1495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000226 DAZKOB010000000
VERSION     DAZKOB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.235"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..1205)
                     /locus_tag="ACG8M6_003030"
     CDS             complement(66..1205)
                     /locus_tag="ACG8M6_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0458597.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          396 a          317 c          271 g          511 t
ORIGIN      
        1 taaacagagc tggattagca aaataatgct aatctggctc tgttttttca tttattaaaa
       61 aaatcttacg ctaagtagga taaaataatt ccttgatatt tttcgatcat atccaaataa
      121 ttatcgattt caacatactc atctacttga tgaggcaaag taacgacacc aggcccaaag
      181 acgacaaagt cgaaggaatg gttagctttc gtaaattcgg ctgcatccgt tgttgctgcc
      241 gctccaacta aaggtaatgg ctggctgaat tgttgctgaa tggaatgaat caaaggagaa
      301 tctggatcag ctttgacagg tattttgttg taatcgatca tcaattccaa atgatagtct
      361 gtctcttgat tcaattcatt gacgattgat tgcagcaatg caatgatttt atcatttggg
      421 tattctggaa tcgatcgaat gttcccttgg agctgcgcat ggcttgggat gctgttgact
      481 tgattcccgc cactgatcaa tgtaacatta tggattgttt ttcctaggac aggattctcg
      541 attgtttcag cgaggtgatt catttcagca tttgctttcg taatgaattc atttaggtga
      601 ttgattgcat tataaccttg gtctggcata gagctatgcg cttcttttcc gtgcgaggtg
      661 actgtatagt tgattgatcc catgtgcgta tacatcaaac tgtaattggt tggctctccg
      721 atgatcaagg catctaagtc atctacatag cctgctttcg tgagctgttc cccacctagt
      781 tcacccactt cttctccgac agtggctaat aatttgactg ttccgttaaa tggttttcct
      841 gactctttca gttcgatcat ggcaatcacc attgctgcca agccactctt catatctgta
      901 gcaccacgtc cgtacaaacg gttcccatgg atttctgctg caaagggtgc ataggtccaa
      961 gacgattcat caccagccgc cacgacatcc atatgtccag ataggcctaa cacttttcct
     1021 gattggccct tttggtaacg agcaatcaaa ttatctctac catctctgta agaaacaatc
     1081 tctcctgtta tgtcatgcct tgccaaaagc ttattcaaat aggctgctac ttcaccttcg
     1141 tttccattca ctgatttaat tcggataatc tcttgtaaaa tcgcgatttt ttcttctttt
     1201 ttcatctaat cctctccctt gccatttctt tttattgtat ttgagtgtgc tcgaattttc
     1261 aaataggagc aaccaaagta aaaatgaaga aagagagcca ctttatgaaa aatatgctgc
     1321 tacttccctc ctattttatt tatacgagag gacttgccca tagattccta ttctactgtt
     1381 ctatccttaa aagctcttat atgatgctag ttattgttag ctttcctttt tcaaggatga
     1441 tcttttcttg taccattctt tgtaaacatc atatacagaa gctagttgca aaaaa
//
LOCUS       DAZKOB010000227         1460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000227 DAZKOB010000000
VERSION     DAZKOB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.236"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..405
                     /gene="traE"
                     /locus_tag="ACG8M6_003031"
     CDS             73..405
                     /gene="traE"
                     /locus_tag="ACG8M6_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0458598.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1024..1365
                     /locus_tag="ACG8M6_003032"
     CDS             1024..1365
                     /locus_tag="ACG8M6_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0458599.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          527 a          188 c          321 g          424 t
ORIGIN      
        1 caattctcta tttttgaaaa ataacaagaa aaacttgtta aaaatattta aagaaattgg
       61 gtggtttatt ttatgtcata tacaggaatt ttgagtctca aagatatttg tcactacggt
      121 aaacggtgta ccgctacaga gaaaatcacg aagaaactat caactggcca aagtaaaacc
      181 gttgtgcagt gtaagaaata tataatccag aaagacaaag tctcagagga aatgatttat
      241 tacattggga aacaaaagca aataatttta aaagatccta tccccttaaa agagctatat
      301 ccaacaatca aacatgttta tgaccaaaac ggtgtactga ttggtcgaag aaaaaatgga
      361 gtcttgcgtt gtaccgcaaa agggatgggg cgtttgatcg gctgaattga tcattctcat
      421 aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg tcgagacttc taaaggaact
      481 tagagtcact agaaaaataa ggagtagact tgatctatca acaattccct tcggggagtg
      541 acggggtagg ttttaacgat tggtaacgta aaaaattggt caatcgagta aatgcgcttg
      601 tttggatagt ggtgaaatga atttgtgtaa ataataagcg cagagtaaac aagagggaat
      661 ttagaagtta gacaagttag aatagcgtct agattaatca tgagcagcta ctaagggatc
      721 tagactttga cgcaaactca tggaataagg agtaggtctt ctcgtaatgg ggaggacgca
      781 caagaaattg tgtcgtgtgc tgcagtacat gtagatactt gcgaggacgt tgattattgg
      841 atcaatcgag catttaccgc tgtctactag ggtttctcgt ttcggacgcc ataaacgagt
      901 cgaaattttt tatgtatttt taagtaaacg aaaaacgata aaatatgaac aagtcctgaa
      961 taaagtgaaa aaattaatat aaagcataga gaaaatgaaa tttacgtaat ggaggaatga
     1021 caaatgaaaa attatgatcc gaatatacga ttgggcacac atactattaa ggtttctttc
     1081 caacgttggg attacaaagg atttgtgacg tttagaaggg gcggaaattg taaaggatta
     1141 gatgttttag cattagacga ggatgattta tacgatcaaa cattgactga taatccaatt
     1201 ggattcggct tgctacctga agatgatgaa ggtaatgaat ggtttaaaat gacgcttatg
     1261 aatgataatg gagacgaatt atctgttgaa gatacttggt catatttgag tgattatatt
     1321 gtcagctttg aaatcattga atttgtagcg gataaagaag aataaaaact ggaggaataa
     1381 attttacaga taaaggctta gaaggagtca cgacgattaa taaaaaggta gtattacctt
     1441 aggcattgag taaggaattt
//
LOCUS       DAZKOB010000228         1440 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000228 DAZKOB010000000
VERSION     DAZKOB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.238"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            474..725
                     /locus_tag="ACG8M6_003033"
     CDS             474..725
                     /locus_tag="ACG8M6_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458600.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="ACG8M6_003034"
     CDS             759..1037
                     /locus_tag="ACG8M6_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458601.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
BASE COUNT          513 a          197 c          267 g          463 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
//
LOCUS       DAZKOB010000229         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000229 DAZKOB010000000
VERSION     DAZKOB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.239"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8M6_003035"
     CDS             complement(150..1337)
                     /locus_tag="ACG8M6_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0458602.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKOB010000230         1376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000230 DAZKOB010000000
VERSION     DAZKOB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.240"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..340
                     /locus_tag="ACG8M6_003036"
     CDS             <1..340
                     /locus_tag="ACG8M6_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0458603.1"
                     /translation="YFSVVSARRNPYFNMVKRIENEVTIVNKSRYTSRQQTPEVFEMN
                     ASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSEL
                     QDIYNYLYRR"
     gene            345..1292
                     /locus_tag="ACG8M6_003037"
     CDS             345..1292
                     /locus_tag="ACG8M6_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0458604.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          456 a          198 c          279 g          443 t
ORIGIN      
        1 ctacttttca gttgtttctg caagacgtaa tccctatttt aatatggtaa aaagaattga
       61 aaatgaagtt actatagtta ataaatcaag atacacatct aggcaacaga cacctgaagt
      121 ttttgaaatg aatgcctcaa tgtattcttt taaacctgaa tttctaaaaa aaagtaatta
      181 tatttttgat gggcaatgtg ggataataaa aatgagagat tatctaattt tagatattga
      241 tagtgaagaa gattttaaat ggttagaatt tttgtttcct aaatttttag aagattcttc
      301 ggagcttcaa gatatctata attatttata tcggaggtaa aaggatgagc tgttatctta
      361 tcaccggtgg ggctggcttc attggttcta ccttagccaa tcatttagga gaagaacatg
      421 tggtaatcgt cgtcgatgac ttatcaatgg gaaaagtaga aaatcttgat atgacgaaaa
      481 atatcacttt tattgaggga gatgtcgctg agaaatcctt gatggaagag atcatgaaag
      541 ctaataaatt tgattatatc tttcatttag cagcagttgc tagtgtggca gattcagttg
      601 cccgaccagt agaaacacat cgtgtgaatt ttgaaagtgt attaatgatt ttagaattga
      661 tccgcaagta tcaacctgat ttaaaacgga tcgtgtttag ttcttcagcg gctgtttatg
      721 gggatgaacc gactttacca aaaaaagaag agtccgtgat tcgcccattg acgccttatg
      781 cggttgataa attcgctgca gaacaatatg tgctagatta ttgtcattta tatgatgtac
      841 ccggtagcgc agttcgtttt ttcaatgtct atggtccaaa tcaaaatccg aattctccat
      901 acagtggtgt gatttctatt ctcgtagatc gctataagaa acaaatcgta ggagaagcaa
      961 cttcatttac attatttggt gatggaagcc agtctagaga ctttgtttat atagatgatg
     1021 taatccaagc attactttta gttgcgaatg aggaagctgc actggggaaa caattcaatg
     1081 ttggaacggg taaagcaacc acacttttgg aattgattca tgcaatcaat gaaattctcg
     1141 gtgtagaatt gacacttgaa tacaaagaag agcgttcggg tgatatccat gactctctag
     1201 cagatattac taagatccag tcaattggct atcaaccgaa atatggtatt ttaagtggaa
     1261 tgactaatta tttgaaaact gaaatcaatt aaaacatact aagataagat tagagggtaa
     1321 attcaggctc tttgtcaata aggactgatg agttttgtaa aatcaaatct gggata
//
LOCUS       DAZKOB010000231         1364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000231 DAZKOB010000000
VERSION     DAZKOB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.241"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACG8M6_003038"
     CDS             82..>1364
                     /locus_tag="ACG8M6_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0458605.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAZKOB010000232         1362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000232 DAZKOB010000000
VERSION     DAZKOB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.242"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..484)
                     /locus_tag="ACG8M6_003039"
     CDS             complement(<1..484)
                     /locus_tag="ACG8M6_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0458606.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     L"
     gene            complement(474..>1362)
                     /locus_tag="ACG8M6_003040"
     CDS             complement(474..>1362)
                     /locus_tag="ACG8M6_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0458607.1"
                     /translation="IWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQ
                     TWIDLKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVT
                     GIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNAL
                     KDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNL
                     VKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTE
                     LTRQMKRDAGAVGGTLYAT"
BASE COUNT          434 a          244 c          163 g          521 t
ORIGIN      
        1 taagcggttt tgaagaatac ttctctgggt tataaatttc aaaagtttct gtttgtcgaa
       61 tagtaaatct accattgtaa ttttccttaa atggtcgaat actcactgta aattcaaaag
      121 ggacaatatt tcctgttttt cttgttcctt taaattcagg tccttctatt actacagctt
      181 gataaatata ttgttcatca tagtaaagaa taaaatcact ataagacgac atatctaacc
      241 attcagtgat tcgatcttcc cactcctgca ccatatcaat tgaaggtgct ttatagtaac
      301 actcaatctt tctagtcaca tttttgtaat agcctttatc aataatgata gaatcatttc
      361 cttctcgctc tctcaattca attactcgac tagctgaaac agaagcaggc ctgttttgaa
      421 tatacacatt aaattcagaa gaataatgtt gattaataaa aaattgtcct ctcttaagtt
      481 gcatataatg ttcctcctac tgcaccagca tctcgtttca tttgtcttgt caattctgtt
      541 ttaatttttg tggtaatctt ccgaaccatt gaatcaggta aatctccata aacatttaaa
      601 tgtaaatgaa tttcttttgt ttcactagca gatatatttt gctttgtttg tgtaattggt
      661 aggtgactta ttccatttac tacaggttga acagacggtg tttttactaa atttgtcatg
      721 gtttgatcta acttactttg ctctttatca ataccaacga taataccttg aacaatattt
      781 ttaccaacca tatcacgcat ccatcttgat ggagaatgaa tatctaaagc tcctttaatc
      841 caatttttga tattacctgc aatacctttg atagtatctt tcaaagcatt ccacttttct
      901 tttacaccat ttataagtcc atcaatgatg ttcttgccaa tttcaaataa attgacttcc
      961 cttaacgaat taaagatttc ttttacccga ttaatagcat ttgaaacacc attttttaga
     1021 tttgtccatg cattttcagc agaattaaca attccagtaa caatattcca gaaagagtcc
     1081 ttaatgttat tccatgtatt aatcatggta ttttttatag aaatccacgt attgtatgcc
     1141 gtatctttaa tatttttcca agtatctttg aaaaactgtt taatattatt ccaagtagta
     1201 atggcattat attttaaatc gatccaagtt tgaataatag aaaacttcaa ttcaatccat
     1261 ttctgaattg caaaatattt tatatcaatc caaagattaa taaaaaaata ctttatgttt
     1321 aaccaaatcg atttagcttg atttaccact tcattccaaa ta
//
LOCUS       DAZKOB010000233         1294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000233 DAZKOB010000000
VERSION     DAZKOB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.243"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1244
                     /locus_tag="ACG8M6_003041"
     CDS             <1..1244
                     /locus_tag="ACG8M6_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0458608.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAZKOB010000234         1259 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000234 DAZKOB010000000
VERSION     DAZKOB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.244"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..>1259
                     /locus_tag="ACG8M6_003042"
     CDS             73..>1259
                     /locus_tag="ACG8M6_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0458609.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LEC"
BASE COUNT          392 a          247 c          247 g          373 t
ORIGIN      
        1 aaaaaaatat agaaaagaca ccctgaaatc ctttagaatt aatttggcta ccaattcata
       61 ggaggattca agatgtctca tactcagctt atcaaagata ccctaaatat tctagaccta
      121 aatatctatt tcgaggaaaa ttgtttaact aaagaaaaat acaaagggca aatttgcttg
      181 atttacaaag gaactcttct gtacaagcca gaagagtgtt cccattgtct ttgtgtcgtc
      241 ccctctagga tcattcgttg ggggacgaca accgttcggt tgttactgaa tgatgtgtct
      301 gagtatcgca cttatctgga gctgaagaaa caacgattta aatgtaagtc ttgccaaaga
      361 acattcgtag cggatacctc tgttgctgaa aaacactgtt tcattagtca aaaagttcgt
      421 tggtctgtca ttgctcgctt aaaagagaat acttccatga cggaaatcgc tcgtcagaaa
      481 aatatttcca cttcttcggt ctatcgtgtc atgaagcgat tctaccgccc attgaatcct
      541 ttcaaacaga ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt
      601 gccagtgcaa tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc
      661 gaaaatcgac gcttgcctta tttagaacgc tatttttctc ggttttcatt agcgactcgt
      721 gaagccgttc aattgattgt tattgatatg tatgcgcctt atgtttcgtt ggtgaaaaag
      781 cttttcccga atgctcagtt gatcatcgat cgtttccaca tcgtccaaca tattggcaga
      841 actttcttga atcatcgagt gaaagaaacc acggtacgac ttaaaggaaa ttcccactct
      901 caacgagggt tagggaaaaa attgaagcgc tattggaaac tcctacaaaa agaagaagca
      961 aagttaaact atgaaaaacg ggtttggcgt gccagtttta aagattattt gactgaaagt
     1021 gagattgccg atcgactgtt agctgcttgt cccaacttac gccaaggcta tcagttatac
     1081 caagacattc tttatgcggt aaagaaaaga gatcagtcac tatttgagga ttgtttaact
     1141 agagacatta gcggtctgcc agaaacctat acaaccactt tacgaacgtt taaaaagttt
     1201 ctgccacaga tccaaaatgc gcttcactac agctattcaa acggaccatt ggaatgctt
//
LOCUS       DAZKOB010000235         1258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000235 DAZKOB010000000
VERSION     DAZKOB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.245"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACG8M6_003043"
     CDS             complement(<1..426)
                     /locus_tag="ACG8M6_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458610.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSE"
     gene            complement(443..>1258)
                     /locus_tag="ACG8M6_003044"
     CDS             complement(443..>1258)
                     /locus_tag="ACG8M6_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0458611.1"
                     /translation="LILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNY
                     DPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEK
                     LAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHL
                     SPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKIST
                     AKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          431 a          217 c          137 g          473 t
ORIGIN      
        1 ttcactcaaa tacttataat ctattaactt gtagttgttt aacttttctg aaaaagttag
       61 acccacgtaa taatcttccc agctttctct ttcaattaac tgatccaatt tttgataatc
      121 cataaattca tcatgtatca aaaagaataa ataacttgat attttagcat ctagcttaat
      181 cagattaaat aatgtttgtt taaaattgct accattaata tgactgatag attctctagc
      241 ttcttcacat aaacttttta ctttattaat tactactttg tcatattcat ttaacacact
      301 attattacca ttacttagac gctcagattc tacgaaacaa ctcaaatcag aaatttccca
      361 aaaaaatttt ttgttaaaat ccaatttcct caactcctcc aaactacgta aatgatatag
      421 tctcataata aaataaaacc tctcagctaa tgttgttgaa attgtccact aaacctaatt
      481 gatattgctt aggagataca cctgcgtact ttttgaatac tctcgaaaag tgtgaagaat
      541 ctgaaaatcc taaacctaaa gaaacttcac tgatagtatg gtttgatttc aacattattt
      601 tagccgtaga aatttttcgc ttagagatat aattataaat cgtaatatgc atttcattct
      661 taaagctttc tcttaattta gtttcactta cagaaaaata attagctatt tcctttacag
      721 taattctttt atagatattt aagccgatga attgaacgac agaagaaatt aagggagaaa
      781 gatgctctat cttagttttt ccgatttcct tagtataata aacaatccca gaatccctca
      841 cttttaagac ctcaggtaac gatacagcta attcgttttc ttgtatcaaa gcatctctac
      901 tttgatacgc ttctattata tccattccta atgttataga cgtttgtgct aacttttcaa
      961 aaacaatgat agaataattt ttttcagacc gaatcgtatc tccactaaca cttggaatta
     1021 ttccattact cagattaaaa accatgtttt ctaaatttcg aatatctcca tttttaacat
     1081 attccagtat tttattttca taaagaaaca gatatatttc tggatcgtaa ttttgtgata
     1141 acagattatc tattttgatt tgctcgatag ttttacttag attttctgta tattcaatta
     1201 acggttcatg gaatagatct tctatttttc cagtaaaaaa ataatgaact aaaatcag
//
LOCUS       DAZKOB010000236         1249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000236 DAZKOB010000000
VERSION     DAZKOB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.246"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..>1249)
                     /locus_tag="ACG8M6_003045"
     CDS             complement(108..>1249)
                     /locus_tag="ACG8M6_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0458612.1"
                     /translation="NFGYLLITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAAL
                     ISIYQFIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVL
                     ISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREV
                     LPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKE
                     VILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTD
                     VNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIV
                     LIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          492 a          206 c          149 g          402 t
ORIGIN      
        1 cagaataaat aaataaatct ccttttgatt ctcggtaaaa attttgataa acatttaata
       61 tattaatcaa atgtaaagga gtccaagcaa tgaaagctgt cttcatatta attctccttt
      121 tttaaataca agtaagaaat gcttcccaaa taataccaga aaacaattga aatgacatcg
      181 cgcttattga aaagtacatg agcctccacc atttcatcta taaaaataga tagtataaaa
      241 attaaaacaa taccaagaaa cgcataattc actttacttc taaaaaatgt tttgagtaaa
      301 ctaaagatat ttaacacata gaaagcatag atacatataa aagcaattat accacttgat
      361 acaagtactt gaagaaatcc attatgcatg tttccatgtg ctctttttag ttcattcaca
      421 tctgtagtat caagatctac ttttttcaaa aattttgcat cgctattccc atagaaatcg
      481 gtctctccat aaccaaataa caaagttggt cttataactt taagcgcagc tttccaaatc
      541 gtaaaacgac cagctgaaat ttcggagtta tcactagaat gctgtaatat tacttctttt
      601 tctggaagtg gagttttatc tgattcattt gactctataa cttctacttt tctatcaatc
      661 gatacaggaa ttgttgctaa aaaatcctta gcataattag taccaaaaga aaaatatacc
      721 aatagaacta ataatatgct taccatccat ttgattttta caggtagtac ttctctaatg
      781 ctctttctaa attttgaatt ggcaaacaac aaaagaaata atactaaaaa agaacaaatt
      841 actacctggg cacctctaga cgaagaaagt gtaatataaa atatttgtac gataaaattc
      901 atcatataaa aaacacgaat cgtttttgtt atttttgaaa ttaatacact aattaaactt
      961 aaaataatag aagtatatcc aaacatagct ccaacatttg gacttgtata gacaccaaat
     1021 aaccgatgtt caataaaacc ttgtcttgca agtaatcctg gcgttcctgt tgggacatga
     1081 taagaaatca aaaaaataaa ttgatagata gatattagcg cagctaaaaa aattatgata
     1141 atgaaaacat tattaaaata caccattttt ttcttttttt cctcttcact ttgtgaaaag
     1201 tctgcaggaa aaattactat caaagtaatc agcaaatatc caaaattat
//
LOCUS       DAZKOB010000237         1193 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000237 DAZKOB010000000
VERSION     DAZKOB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.247"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8M6_003046"
     CDS             <1..1076
                     /locus_tag="ACG8M6_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0458613.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAZKOB010000238         1160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000238 DAZKOB010000000
VERSION     DAZKOB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.248"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(489..863)
                     /locus_tag="ACG8M6_003047"
     CDS             complement(489..863)
                     /locus_tag="ACG8M6_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0458614.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(886..>1160)
                     /locus_tag="ACG8M6_003048"
     CDS             complement(886..>1160)
                     /locus_tag="ACG8M6_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFQ0458615.1"
                     /translation="NNQLTFGKTPNYPWPQMFDKGLQRPLFLEIGKEYELKLIVDDTI
                     AVLYIDNVALSTRMYDKVGTALSMTVTEGAIKVDKLDYSINYECGF"
BASE COUNT          385 a          218 c          151 g          406 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag gattacaaaa tttgaaacca
      361 aataagtaaa aaatactgtc actttgaatt aatttgaaat agtaaattct cattaaacta
      421 ttgaaatgct gataccaaaa aatgaagtac ttttcgttca taccaactta gagcacttcg
      481 ttatctattt atttcttcat ttggcttatc tctataatct catgatttgg tccgtagaaa
      541 ttaaagtaac ggattccatt ttcccagaac ggtaattcca taatattttt atctaataat
      601 tcatatccta aatcttgaat tgaattgaat acctcatcta tgtcatccgt atctaatgct
      661 agatgatcaa tggctcctct acactcagta gttttatctt cccatatttc tattatcgtt
      721 ccattcaact cgaaaaagcc gactttactt cccacattca tttcttcatt aatcagacta
      781 aaccctaagg attcataaaa gatttttgaa gctccaaaat cttttgttgg aattccaata
      841 tgttgtaaac catttaattt cacttacatc atcccttcaa ttgttttaaa atccacattc
      901 ataatttatt gaatagtcta gtttatctac cttgattgct ccctccgtaa cagtcatact
      961 taatgctgtt ccgactttat catacattct agtacttaaa gcaacattat caatatataa
     1021 aacagctatc gtatcatcaa caattaactt tagttcatat tctttgccta tttccaaaaa
     1081 tagtggtcgt tgcagcccct tatcaaacat ttgaggccaa ggatagttcg gagtttttcc
     1141 gaaagtaagc tgattattaa
//
LOCUS       DAZKOB010000239         1159 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000239 DAZKOB010000000
VERSION     DAZKOB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.249"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG8M6_003049"
     CDS             <1..969
                     /locus_tag="ACG8M6_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0458616.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          393 a          204 c          232 g          330 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccag
//
LOCUS       DAZKOB010000240         1150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000240 DAZKOB010000000
VERSION     DAZKOB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.250"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..302
                     /locus_tag="ACG8M6_003050"
     CDS             66..302
                     /locus_tag="ACG8M6_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0458617.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            321..536
                     /locus_tag="ACG8M6_003051"
     CDS             321..536
                     /locus_tag="ACG8M6_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0458618.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT          377 a          166 c          248 g          359 t
ORIGIN      
        1 gatttttact gttaatcaaa tacgtatgtt acagtagcac cgtaaaaaat attatttaaa
       61 gaagcgtgtt taaacatgca aaatgtaaag gaactaagta ttgtggaaat gcacaaactg
      121 ttggtggagg caataatgct tggggaaaat tggggcaagt tgtaggtgga cttaccacag
      181 gggcagtagg tggtgcaggt ttaggaacag caatttgcgg accagcttgc ggtgttgttg
      241 gagggttata tggagctgtc gctggaggtg ctgcagctgg ctgggatgct cgaaaaaaat
      301 aaaaaaggag aataagtagt atgcatatta aaaacacaaa aacaacattt atattatctt
      361 cagaagagtt gaaaaatatt caaggtggga gtgccgtggg agttttaggc actacttttt
      421 ctggggcaac agcaggagta aaactttgtt cagccggagg accttatgca atagctgctt
      481 gtggtgtagg tggagcttta ttaggtgctg gattcagcat gtggaccggt gcgtaaacaa
      541 ttaaaaaata tcaaacctca agattggttt gtgtcgatta cgaacatagg tatgttcttt
      601 atcttttcaa aagtttggaa tggacagaac tttttactaa agcttcttgt gctagttttt
      661 ataattatat taaattttta tcttttatta aagtcagata aaaattcttt ggaaatgtaa
      721 tggtagagat atttttacaa aaggacttgc agcgctgtag ttggtgctgc aggaggacct
      781 atttcggccg ttacaggagg tattggtaat gtacttgtcg gatgtgcatt aaagcctgtt
      841 cctgcaagtt aaatttataa aaacaggaaa gtaattatct caaatcctat caagtacaat
      901 cataggattg cacttgacag gacaaatttt ttaactaaaa aatgaaaaac actttctaaa
      961 aattagtaga atttgccaaa tctttcgcat cttccaccat acatttaatt ttaattcccc
     1021 tttatggtac ttaataaaaa caagaaaaga actattcatt tggccctttt ttggttccag
     1081 accatagaaa tattcctcca gtaactaata ttgctgttat gcacgtaact aataacagct
     1141 ataaaaattg
//
LOCUS       DAZKOB010000241         1137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000241 DAZKOB010000000
VERSION     DAZKOB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.253"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG8M6_003052"
     CDS             <1..355
                     /locus_tag="ACG8M6_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458619.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG8M6_003053"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG8M6_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="ACG8M6_003054"
     CDS             985..>1137
                     /locus_tag="ACG8M6_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0458620.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAZKOB010000242         1124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000242 DAZKOB010000000
VERSION     DAZKOB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.254"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..277)
                     /locus_tag="ACG8M6_003055"
     CDS             complement(68..277)
                     /locus_tag="ACG8M6_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458621.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(344..583)
                     /locus_tag="ACG8M6_003056"
     CDS             complement(344..583)
                     /locus_tag="ACG8M6_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458622.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            993..>1124
                     /locus_tag="ACG8M6_003057"
     CDS             993..>1124
                     /locus_tag="ACG8M6_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0458623.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          298 a          227 c          165 g          434 t
ORIGIN      
        1 tttttcatcc atctgcctcc tttcagacaa aacaaaaaaa ctttttctca tttccataga
       61 aaaagtttta aaaatgaatt ctataagttc gtctcaactg ttcaaagttt ttcattgaac
      121 ctaacaaaca agctttctct tttcctgtta actgcatatc attgatatat tgcgcgacag
      181 ataatctttt aaggtgtttt tgcttcatcg tttttagtaa cttttcttcc atttctttcg
      241 acactttttt tctgttttcg atttcttcat aattcatgag ttttcctacc ttccttgttt
      301 tttgcaaaaa taaaaaagcc tatgatcaca tacgctctac acttcagtct tgggttatct
      361 ttttctttcg tttaattact ggccgtgctg gtttcaaatc cttgattcca ttatgggttc
      421 tccaataatc gaattctttt cgcaatcttt ctttttccat atctgttcct ttagataagg
      481 ctttgaatac ttctttaggt gaataaccac gagctaacag ctgtacatac cttccttttg
      541 tcaaagtatc catatccatc gcctctaaaa ctggtcgcgc catctactat tcctcctcgt
      601 aaaatatcat ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga
      661 ataacacatt cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat
      721 gtatggaaag gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt
      781 tagtctgtta cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac
      841 tagactttca aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt
      901 aggcgaggtt gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc
      961 caaaatttat atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta
     1021 gctctgaaaa actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg
     1081 aaatctctgt tgccaatgac atcgaaggtg cttcattcat acga
//
LOCUS       DAZKOB010000243         1101 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000243 DAZKOB010000000
VERSION     DAZKOB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.255"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1101
                     /locus_tag="ACG8M6_003058"
     CDS             <1..>1101
                     /locus_tag="ACG8M6_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0458624.1"
                     /translation="LINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLS
                     LATLSLIVFLLIGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHF
                     KYLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAH
                     FFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILS
                     VIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPK
                     DYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIP
                     RLGIIAAGYTTVFTYFLLFIFHYNIAKKITNK"
BASE COUNT          338 a          139 c          179 g          445 t
ORIGIN      
        1 gtttgattaa taatttcact tcatttgtat caattttttc gattcttgtt gggctaagcc
       61 taaattcggc aattaataat gcaagttttg actataaaga tgacataaat gaatttatgt
      121 catcggtact ttctctagct acactttcgt taattgtgtt cttattaata gggaacatat
      181 attttttgtt ttcaaatgct ttttttgata tgaaccaact tgtatttaat ttgatgttat
      241 tacaaagttt tgctagtttt gtaattagtt atatccagtc ttattttacg attaacttta
      301 accattttaa atatttgatt ttatctttta tatctacttt tttgaatatt gcaatctctg
      361 tagctttgat gcagacatta tattttgatg ataaatatgt tggcagagta gtcggcggaa
      421 cactgtcatt tgtgttgata ggtttagtaa tttatttaag aataatgata aagggtgctc
      481 atttttttga taaaaaacag tggagatacg ctttaaaaat ctctttgccg ctaatcccac
      541 attccttagc aaatatagtt ttagcacaat tcgatagaat tatgattaat tcttatttag
      601 gtcctgctgc ggggggaata tattcctaca ttagtaacat aggtattatt ctaagcgtaa
      661 tatggatgtc tactaataac gcctgggttc catggttcta tggtgagatg agtaaaaata
      721 atgaaaaaaa agtgaagaaa gttagtaact attatttagt agtctttact ttgattacga
      781 tgattgtaat ggtagtttcg attgatttag caaaatttat ggctccgaaa gactattatt
      841 ctgggttatc tttagtggtt ccgataacct taggctattt ctttcagttt ttatatagtt
      901 taccagttaa tgctgaattt tatgagaaaa aaacatcgtt tattgcgatt ggtacagtag
      961 caagtgctat agttaatata ctattaaaca tgattttcat acctaggtta ggaataattg
     1021 ctgctggata cacaaccgta tttacgtatt ttttactttt tatttttcat tataatatcg
     1081 ctaaaaaaat tacgaataaa c
//
LOCUS       DAZKOB010000244         1096 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000244 DAZKOB010000000
VERSION     DAZKOB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.256"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1096
                     /locus_tag="ACG8M6_003059"
     CDS             <1..>1096
                     /locus_tag="ACG8M6_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458625.1"
                     /translation="WYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHI
                     NRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIV
                     TLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLIL
                     LGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLI
                     IKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSEL
                     LSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNII
                     QAPWRFMIFSTLFISFSVSLFISPQLEKFS"
BASE COUNT          345 a          150 c          148 g          453 t
ORIGIN      
        1 ggtggtatat gaatttgaaa aaaatttttt ctgtaaaaat atttatagtt tatgcatttg
       61 ttagtttatt atatttattt ccagcattta aaaacatgga aatatttggc ttttatgata
      121 cggcatttca cattaatcgt gcgttaagtt tagaatctat ttttagctcc cctattaatt
      181 ttgaaacgtt tagatcatat ggcatgcaag taaataattt ttacccatgg ttaactctat
      241 atcctttatt tttattgatc aagtttacta atttagcaat aggctataac ctttttcttt
      301 atattgttac gctcattact ctatttatat gtcattatgt aatgtatgaa attaccaaaa
      361 aacatgttac tagctccttt ttcgctatta tatatacaac atcttctttt aggagtgtag
      421 aaatatttct tcgaggggca atgggggaat tactcgcaat gtcaatactt cctcttattt
      481 tactcgggtt tataaagctt tacgactcaa aaaaagaatc ttgggttatg ttggcgatta
      541 gcatgacttt attaatttac actcatgttc tttctgtagc tatgactatg gtaatgatta
      601 taattgcttt aattattcaa ttttatcgga aaaagaaaat agaaatcttc ttaattatta
      661 aattaataaa agctgctata gttactttac ttctttcact agcgtttata ggtcctatga
      721 ttgagcagac attgtatcaa gatctgaata gaccttatgt ggatatatta caaaaaagag
      781 caatttatct gggtaaagag tttttgattg gaagtataga ttcagaatta ctatcatttg
      841 gtattggctt aactttgctt atttgtttaa ttatactaac atttaacttt aaaaaattag
      901 gaatcttatc taaaatcact tatataatgg gtataatatc tattctactt tgtacaaaaa
      961 tatttccatg gtttgcctta caaaatactc ctttaaatat tattcaagct ccttggcgtt
     1021 ttatgatatt ttcaacattg ttcatatctt tttcagtatc tctatttatt tctccacagt
     1081 tggaaaaatt ttctta
//
LOCUS       DAZKOB010000245         1073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000245 DAZKOB010000000
VERSION     DAZKOB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.257"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1073
                     /locus_tag="ACG8M6_003060"
     rRNA            <1..>1073
                     /locus_tag="ACG8M6_003060"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAZKOB010000246         1067 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000246 DAZKOB010000000
VERSION     DAZKOB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.258"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8M6_003061"
     CDS             complement(170..907)
                     /gene="erm(B)"
                     /locus_tag="ACG8M6_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0458626.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          311 a          171 c          187 g          398 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc ccgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta atggtactta
      241 aattgtttac tttggcgtgt ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca acaatcagat
      541 agatgtcaga cgcacggctt tcaaaaacca cttttttaat aatttgtgtg cttaaatggt
      601 aaggaatatt cccaacaatt ttatacctct gtttgttagg gaattgaaac tgtagaatat
      661 cttggtgaat taaagtgaca cgagtattca gttttaattt ttctgacgat aagttgaata
      721 gatgactgtc taattcaata gacgttacct gtttacttat tttagccagt ttcgtcgtta
      781 aatgcccttt acctgttcca atttcgtaaa cggtatcggt ttcttttaaa ttcaattgtt
      841 ttattatttg gttgagtact ttttcactcg ttaaaaagtt ttgagaatat tttatatttt
      901 tgttcatgta atctctcctg aagtgattac atctataaac aaatacagaa gttaaacgat
      961 ttgtttgtaa ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta
     1021 atctaacgta tttatttatc tgcgtaatca ctgtttttag tctgttt
//
LOCUS       DAZKOB010000247         1008 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000247 DAZKOB010000000
VERSION     DAZKOB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.259"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..988
                     /locus_tag="ACG8M6_003062"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACG8M6_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          354 a          181 c          200 g          273 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgc
//
LOCUS       DAZKOB010000248         1002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000248 DAZKOB010000000
VERSION     DAZKOB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.260"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            369..1002
                     /locus_tag="ACG8M6_003063"
                     /pseudo
     CDS             369..1002
                     /locus_tag="ACG8M6_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DAZKOB010000249          993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000249 DAZKOB010000000
VERSION     DAZKOB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.261"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..451
                     /locus_tag="ACG8M6_003064"
     CDS             41..451
                     /locus_tag="ACG8M6_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0458627.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            465..821
                     /locus_tag="ACG8M6_003065"
     CDS             465..821
                     /locus_tag="ACG8M6_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0458628.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          341 a          154 c          196 g          302 t
ORIGIN      
        1 ttactgtaga attggattat aattaaaaag gagtgagaaa atgataaaag tatatacttc
       61 gcctagctgt acttcctgta gaaaagcaag agcatggttg caagcgaatc aacttgaatt
      121 tgaagagaag aatatttttg ccgaaccgtt aacagaaagt gagatcaaaa agatcttggc
      181 agcgactgaa ggaggagttc aagatattat ttccactcgg tcgaaaattt acgaaaagct
      241 gggcatagat tttaacgaat taactttaaa gcaaatgatc gtcttattca aagaatatcc
      301 ttcattattg agacgaccta ttttgattga tgaaaaaaga atactaatcg gatttaatga
      361 agatgagatc cggtcatttg tccctcgtga aattcgatgt gttgccagaa atctgatcta
      421 tttacaaaac aataacatct taaattcata gggagggagc cttcgtggaa gtgaaagtct
      481 atggatcagc gcactgtgta acaagtctga aggcgaaaca atggttagaa caccatcaat
      541 tgccttatca atttgttgac ttagaagaac gcgatctaag tacagaagaa gcagaagaat
      601 tatgcagttt tgaagaagtg caagcagaac agctatttgc gacttggtct gaggcttttc
      661 acaacattgc gattgacctt tcctgtgtag gaaaaaaaca actgattttt ttatgcacac
      721 aacataccaa tctcctcaga cgtcctttga ttattatcga tgaagttcta tttattggat
      781 acaatgagca gttgatggaa cagcttatct taaataaata gactttaact gttttaatca
      841 agtcactgag catttcatac agttgtaaca gtcgaacact tagcatcaag cgtgttatgt
      901 gttctttttt tgactttatt tttctactat agagacatgc tcaatttatg tggtcaaaat
      961 tcaaatctag gtggggatat agagttaaaa ata
//
LOCUS       DAZKOB010000250          988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000250 DAZKOB010000000
VERSION     DAZKOB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.262"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="ACG8M6_003066"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="ACG8M6_003066"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0458629.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          347 a          128 c          175 g          338 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgtt
//
LOCUS       DAZKOB010000251          961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000251 DAZKOB010000000
VERSION     DAZKOB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.263"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG8M6_003067"
     CDS             <1..459
                     /locus_tag="ACG8M6_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458630.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG8M6_003068"
     CDS             473..904
                     /locus_tag="ACG8M6_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458631.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          362 a          140 c          203 g          256 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 a
//
LOCUS       DAZKOB010000252          931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000252 DAZKOB010000000
VERSION     DAZKOB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.264"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG8M6_003069"
     CDS             85..624
                     /locus_tag="ACG8M6_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458632.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          349 a          116 c          159 g          307 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat g
//
LOCUS       DAZKOB010000253          926 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000253 DAZKOB010000000
VERSION     DAZKOB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.265"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..144)
                     /locus_tag="ACG8M6_003070"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="ACG8M6_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..>926)
                     /locus_tag="ACG8M6_003071"
     CDS             complement(186..>926)
                     /locus_tag="ACG8M6_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0458633.1"
                     /translation="NNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSLKHVQKLM
                     KQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAADITYIPTKKNGWCY
                     LSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREV
                     EQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEG
                     FYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          258 a          161 c          166 g          341 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatt
//
LOCUS       DAZKOB010000254          904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000254 DAZKOB010000000
VERSION     DAZKOB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.266"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>904)
                     /locus_tag="ACG8M6_003072"
     CDS             complement(37..>904)
                     /locus_tag="ACG8M6_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458634.1"
                     /translation="KKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEK
                     SVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIR
                     RELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKF
                     FLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGK
                     PEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQI
                     DDFPEEIIDRIE"
BASE COUNT          291 a          156 c          139 g          318 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 cttt
//
LOCUS       DAZKOB010000255          895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000255 DAZKOB010000000
VERSION     DAZKOB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.267"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            253..876
                     /locus_tag="ACG8M6_003073"
     CDS             253..876
                     /locus_tag="ACG8M6_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0458635.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          330 a          129 c          167 g          269 t
ORIGIN      
        1 aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac ttaaaggagg
       61 tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt tgttgtttct
      121 tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt tttcagcggt
      181 ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata gattggatag
      241 gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca aagtttagct
      301 cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga agacgattta
      361 aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga gctgcaagag
      421 cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga tcgattagca
      481 agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg tgcttctatt
      541 gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag agacttgcta
      601 acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa aaaaatcaaa
      661 gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac tggtaaaaag
      721 attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga aattgttgat
      781 ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt tacaagaaaa
      841 accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag aaaga
//
LOCUS       DAZKOB010000256          837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000256 DAZKOB010000000
VERSION     DAZKOB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.268"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>837
                     /locus_tag="ACG8M6_003074"
     CDS             <1..>837
                     /locus_tag="ACG8M6_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0458636.1"
                     /translation="EVNKLLKKESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHAT
                     EDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYS
                     KNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGE
                     GSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAA
                     KLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTT
                     EQ"
BASE COUNT          326 a          102 c          159 g          250 t
ORIGIN      
        1 tagaagtgaa taagttacta aaaaaagaat ctttttctat agttcacaat caggtttatt
       61 ttggcggggg aattaattta tggttagcca aaaagaatgg aatcagacag agaattgctc
      121 atagccatgc aactgaagat ggaaaatctc aaaatattgt aatgaatgta ttgcgtaaat
      181 ttttaaccaa attgttgtta caaaatgcaa cagactacct tgccgttagt caagaagctg
      241 gtgaaagtct gtttcaaaac cacccctttg agatagttca taatggtatt gatttagagc
      301 tttatagtaa aaattcagaa gctaaggtta ataagagaaa agaattggat atctcaatga
      361 gtacttttgt tgtgggaaac attggacgat tagagaaaca aaaaaatcaa tcttatttgt
      421 tagagatttt tttagaaata ctaaaaatga attctgattc ttacttgatt ttagtaggtg
      481 aagggtcact tagaaataaa ctgaaagcca aagcagtaga attggatata gagaaaagag
      541 taaaattttt aggagaaaga aacgatattc ccgagttatt atctgtgatg gatgtttttg
      601 taatgccttc tctttatgaa ggtctgccaa tggttggatt agaagcacaa gcagcaaaat
      661 taaaattagt cttagcagat actatttcca aagatacaaa gttaacaaat aatgtggatt
      721 ttgtatcatt agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa gagccgtatg
      781 ctaataaaat aacacaagaa ctaaagagtt atgatgtttc gtacactact gaacaaa
//
LOCUS       DAZKOB010000257          824 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000257 DAZKOB010000000
VERSION     DAZKOB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.269"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACG8M6_003075"
     CDS             complement(<1..153)
                     /locus_tag="ACG8M6_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0458637.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
     gene            231..809
                     /locus_tag="ACG8M6_003076"
                     /pseudo
     CDS             231..809
                     /locus_tag="ACG8M6_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT          259 a          152 c          158 g          255 t
ORIGIN      
        1 actcgtttta gattgcttta cccatttgtc aaatgctgaa gccgttagtt catattctcg
       61 aatgatttct acacgtggct ttccagctaa gtaaagattg acgatttgtt gcttgaattc
      121 ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc
      181 tagtctacac accttaattt ttctgtctag ttaattgtag cctatccaca ggaatcaaat
      241 cgcatttatg ggtaacacaa aatatttggg gcgatcatac agatccttgc ggttatcttt
      301 ctgaaatggg tgtaagtaaa gaaaaactag cctatgattt ggctcatggt tttacggatg
      361 ataatccaac tacttcggat gataatccaa ctacttcgga ggataaacca gtaattgatc
      421 caactcgagc aggtgctgca aattctacgc tgacagatgg aacaaattac gcccacgttg
      481 atcagttcgg agaaatcgaa aacgcaaact tgcatgtagc tggatggcac attgctaatt
      541 ataaatacga gtatattttc attatggact acaatactgg aaaagaacta gctagagtaa
      601 gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta ggaaatgttg
      661 gctatcatgt atctttcaat atgcgtgatt tccctaataa gaaagtatac gtcatgatgc
      721 gtgcgacaaa cgatccagaa ggaaatacta agggtggagc acaagatttt catgacaagc
      781 gttggtattt aaatattcca caacgataaa aaattccccc ctct
//
LOCUS       DAZKOB010000258          801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000258 DAZKOB010000000
VERSION     DAZKOB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.270"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..>801
                     /locus_tag="ACG8M6_003077"
     CDS             3..>801
                     /locus_tag="ACG8M6_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0458638.1"
                     /translation="MDTKAYLLRMFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTF
                     FVELFTDVSAAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFS
                     FEGIKGYGYVVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLF
                     VSYVAHQYQVFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKK
                     YTDSLEKDQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIY"
BASE COUNT          274 a           88 c          118 g          321 t
ORIGIN      
        1 ctatcgatac aaaagcttat ctattaagaa tgttcgtggg aaactttttc ttttttctct
       61 tattttatat gactaatact gatcttaaca aaagaaaata tctttttgta tgtttattga
      121 taccactcac tttttttgtt gaattattta ctgatgtttc cgcagctata cctataatcg
      181 gaagttattt tatattaaag gaaaagaaga aaactaatat agtgctttta aataaattac
      241 tattatgtat gcttattgat gatttcagat ctattattac ttccatggtt atgacgtata
      301 tgttttcatt tgaagggata aaagggtatg gttatgtagt attccagata atgcttgatt
      361 ctttgttttt gctgatgttt atttggttat atagaaaatt agctataaat aggattgttg
      421 aacagtccag ttctaaactt actgctatat tcatgattta tttacttaca attggtttat
      481 ttgtttctta tgttgctcat caatatcaag tatttgatca ttttgtgttt ggtgtcttaa
      541 tatttttaat cattcaaatg tttgttgttc tatttttatt tattagaata actattaggc
      601 aacgagaaca atatgaacag caattgaaaa gaaaagaatt ggtttatcta aaaaaatata
      661 ctgattcact ggaaaaagat caagaaaaat ttgccagatt tagacatgac tataaaaatt
      721 tattgctgag tttaaaagaa atcgctaatc aaaaccatga ttcgatattg gaaaaagaaa
      781 taaaagaatt agagatctat t
//
LOCUS       DAZKOB010000259          756 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000259 DAZKOB010000000
VERSION     DAZKOB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.271"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..667
                     /locus_tag="ACG8M6_003078"
     CDS             29..667
                     /locus_tag="ACG8M6_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0458639.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          238 a          121 c          124 g          273 t
ORIGIN      
        1 caaaaactga atagtaggag ttattatcat gaataacatt ttaggaaaaa aaatagaact
       61 taaaaaaggg atattgggca ctattgtcgt tgtactcggt ttgttagggt tgctatttgc
      121 gccatccatt attagcattt tttctttaac tatcactgga tttatagcat ttacacttgt
      181 ttttggtaaa aaagatacga aaaaaatgtt ctcgaaacct gtcgctcctg tcaaaaatat
      241 agccaaatat ttttttatca atgtagtaat cagtgccgca gtctcggtat ttttgcaaca
      301 aattttgaaa tggggtttaa caggcaatcc aataaatgag gctttcagcc cgttattatt
      361 tattattttg cctatcatga tacttggaga agaattgttc tcagtgtatt ttctcgctat
      421 tttttctagt aagttcagca ttcctgtcgc tagtttcttg tcagcaatca tctttggatt
      481 tatccattac agtacttatg ataatggaaa tattttacac actgtagcac acatcctttt
      541 gatacaaggg gtggcaagat tgttattcaa ccaagctgcc attaaaagca actcgatcat
      601 tacaagttgg gcagttcatg tgatatttga tttcacagct atattgctag tcgttttatt
      661 ctcctaaatt tttatagaaa taatacatgc tctattgcta agaaagcata ctttatttcc
      721 tttaaacaaa aagacagatg tatcaatgga ttaaaa
//
LOCUS       DAZKOB010000260          753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000260 DAZKOB010000000
VERSION     DAZKOB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.272"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..>753
                     /locus_tag="ACG8M6_003079"
     CDS             154..>753
                     /locus_tag="ACG8M6_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0458640.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGF"
BASE COUNT          257 a          111 c          125 g          260 t
ORIGIN      
        1 ctaatttgtg tctctcaggg caattctaag aacgtttcaa gagatagcga cactatcgtt
       61 atcgaaaaag gcgaaaacct atctcatttt ctgctaatgt aagttggttt agcttttgtt
      121 aatatgactt tatgaaaatg gaggtaatta aatatgattt ttcctaaagt taatgaagca
      181 agcttgaata aaggatcctt ttttcatatc tatgaaaacg aagattttga actgtataat
      241 atcctttcac aaaaagaggt catcgaaaac caaataatct gccatccaat tatttacaaa
      301 tctattttca agtatgcatt ttttgcttcc aatgattttc aaagaatgga gattaccgac
      361 tgtatattcg aaaactgtga tttttcaggc tgtatattga caaaaagcat gttgcatcgc
      421 gtgaaatttg tcaattgtaa attaacaggt actaaattga tggaaagtta tattgggaac
      481 acgttattcg agaattgtaa aatggactat gtaaatttat ctggttcaaa cataaaagaa
      541 agtacctttg agcactctgt cttatcaagc gcagattttg tagactgttc cctcactaaa
      601 ataatgtttt ctactaatga tttagaaaat gctagctttt ttgatactga tttaaaaaat
      661 atcgatttaa gtagcaactc ctttgaaatg attgaagtac gctcggaaca tctaaaaaat
      721 tgtacgctta atcagttgca agcacttggt ttt
//
LOCUS       DAZKOB010000261          731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000261 DAZKOB010000000
VERSION     DAZKOB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.273"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(333..446)
                     /locus_tag="ACG8M6_003080"
                     /pseudo
     CDS             complement(333..446)
                     /locus_tag="ACG8M6_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          268 a          115 c          112 g          236 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa a
//
LOCUS       DAZKOB010000262          725 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000262 DAZKOB010000000
VERSION     DAZKOB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.274"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG8M6_003081"
     CDS             <1..>725
                     /locus_tag="ACG8M6_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0458641.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGG"
BASE COUNT          226 a          112 c          179 g          208 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggaca
//
LOCUS       DAZKOB010000263          724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000263 DAZKOB010000000
VERSION     DAZKOB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.275"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..631)
                     /locus_tag="ACG8M6_003082"
     CDS             complement(<1..631)
                     /locus_tag="ACG8M6_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0458642.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHS"
BASE COUNT          238 a          143 c           81 g          262 t
ORIGIN      
        1 ttgaatgttg caaatatgaa atatggaata tgatatcttc agatatataa tccctttctg
       61 acggaaactt gatattgtgc tgttgaatta tctcagatga atacagcgca tgccaaactg
      121 acattggcaa tataaaatcg actttataac tcggctcagt gccaatcatt ccaagcaaca
      181 ctttttcaat tatagcgtta tttgtaaata tcgtactatc atatcttgct ggctttgaaa
      241 taatattatt atcatcataa tattcataat atccagcata acatgtgtct gcattactcc
      301 tttttatacc atttaacatt gcttcaatat aattagctgc aacaaaatca tcagaatcaa
      361 taaaagcaac atattttcct tttacatgtt caagtcctgt atttcttgcc aatcccaagc
      421 cagcattatt tttatgaata atacgaatcc ttgaatcctt taatgcatag tcttcacata
      481 ttttattaga tgaatcctta gatccatcat ccacaattat aatatctagg ttcttatatg
      541 tttgattaca cactgattcc aaacaacttc ctacatactt ttcaacatta tatacaggta
      601 tgattacact aattagatca ctcgaattca tccttctatc ctttcactca cgttttctca
      661 attagtcaat gacggcatca acgattttct taaattctct gaaaaaaata gactctgatc
      721 ttga
//
LOCUS       DAZKOB010000264          719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000264 DAZKOB010000000
VERSION     DAZKOB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.276"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..>719)
                     /locus_tag="ACG8M6_003083"
     CDS             complement(334..>719)
                     /locus_tag="ACG8M6_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFQ0458643.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          247 a          101 c          125 g          246 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgat
//
LOCUS       DAZKOB010000265          717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000265 DAZKOB010000000
VERSION     DAZKOB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.277"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..608
                     /locus_tag="ACG8M6_003084"
     CDS             <1..608
                     /locus_tag="ACG8M6_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0458644.1"
                     /translation="IGTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGT
                     GVGGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRL
                     GKKGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFA
                     KLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          201 a          142 c          182 g          192 t
ORIGIN      
        1 tcatcggtac catgaaaaaa gaatttccga tcccgatttc ttggacaaca gatgtcaatg
       61 cggctgccta tggtgagtat gtcttcggca gtggcaaagg attgagcagt gtcgtttact
      121 atacgatcgg tacgggtgtt ggcggcggtg ccttacaaga agggcgcttt atcgaagggt
      181 ttagtcatcc agaaatgggg catatgctcg tcgtccctca tccaaaagat gactttgctg
      241 gcagctgtcc atttcatggc aactgtttag aagggatggc cgctggaccc gccatcgaaa
      301 aacgcttagg gaaaaaagga caagaggttg cagaagatga tccttattgg gaaatcgaag
      361 cctcctatat tgctcaatgt gcctacaata caaccttgat gttttctcca gatgtgatca
      421 tttttggcgg tggtgtgatg aaacaacgcc atatgctgca aaacgtccac gacgcatttg
      481 caaaattggt caatgggtat gtcgaaacac ctgagttaag caaatatatc gtgaccccag
      541 cattagaaga caacgctgga acgctcggtt gtttggcatt ggctcgagaa gcggtcttac
      601 attcataaac gaaaaaaggc tcttcgacat ttagtgttgg tagagaaaga tacaactgaa
      661 aatttagtca taaccagtta ggcagagtgc ttaactggtt tttgttttac ttggaag
//
LOCUS       DAZKOB010000266          690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000266 DAZKOB010000000
VERSION     DAZKOB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.278"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..>690)
                     /locus_tag="ACG8M6_003085"
     CDS             complement(28..>690)
                     /locus_tag="ACG8M6_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0458645.1"
                     /translation="KVFKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIEL
                     AEVIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQ
                     ERIDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRIS
                     ELEKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKES
                     TV"
BASE COUNT          212 a          124 c           82 g          272 t
ORIGIN      
        1 aatgcaatct ccctaaagaa aaaaccacta aacagtgctt tctttcaact tctccttaat
       61 tttttgtgct ttccctaaag taaagtttct ggccaactct gaatcaaaat atagtgaacg
      121 ttttttaaaa tctatttctt taacgttaaa gatatttact aaacacgctc gactaattct
      181 tgttaacata ggatatttct tttcaagctc actaattctt ccataaaatt cgtactgccc
      241 atctttagta cacaatgata atctatgtgg taattttgaa gcttctacaa aataaatgtc
      301 atttatatca aaagtaaatg tttgagaacc tatggaaaaa acaaatgctt gattcgtaac
      361 ctgttttgtt gcatctatcc tttcttgcgc taataacaat aactcaacaa tctcgtttcg
      421 atactcatct agtgtttgat cttttgttac aaatcctaac gtttcaactc ttctttttat
      481 tgttacgggt aacatctcat catgagtagt tgtaaaaatg atttttgctt gaacatcata
      541 ttttctgatt acttctgcta attctatacc gttaacttca tgatttaaat caatatctaa
      601 gaaatatatt ccttgctttg gtcggaattg tttgaggtat tttttaactt ctaacggact
      661 ttgtgttttc aatactatct taaatacttt
//
LOCUS       DAZKOB010000267          684 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000267 DAZKOB010000000
VERSION     DAZKOB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.279"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG8M6_003086"
     CDS             132..344
                     /locus_tag="ACG8M6_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458646.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>684
                     /locus_tag="ACG8M6_003087"
     CDS             506..>684
                     /locus_tag="ACG8M6_003087"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0458647.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSR"
BASE COUNT          245 a          102 c          120 g          217 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtt
//
LOCUS       DAZKOB010000268          684 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000268 DAZKOB010000000
VERSION     DAZKOB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.280"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..263
                     /locus_tag="ACG8M6_003088"
     CDS             27..263
                     /locus_tag="ACG8M6_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0458648.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            260..658
                     /locus_tag="ACG8M6_003089"
     CDS             260..658
                     /locus_tag="ACG8M6_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0458649.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT          246 a          110 c          124 g          204 t
ORIGIN      
        1 gagaatggta tagagggtgg cgaaatatga aaaaaatgaa aaagtataaa attagaaaat
       61 ctggcaatag cgatgtaaca actatcccac cagaagtaaa agaattcatg ggtgtgcaga
      121 caggtgacgc aatcagttat gtttttcaat ctgatggttc tgtacgaatg atcaaagcac
      181 aagaagaacc tgatattgat tcattagttg attcgatcat gaatcaatat gaagacgcgt
      241 taaaagattt ggttgattta tgaggtatct ttcgactaaa gaaattatca aaatcaacgc
      301 gaaagttatt ttacgtcact cgccaggcga aatgatcggt gtaaaagatg caaatgcatt
      361 agatatggcc gtcaaacagc cttcacaagc agtattcaat caagaactct atcctgaaat
      421 ctatgaaaaa gcggcgattt tagcaattaa tttagctaaa aaacatcctt ttcataatgg
      481 caataaacga actgctcttg ttgccatgtt gttgtttctt caattgaata attgtccagt
      541 tgctttttct cgtcaagaag ctgttgattt tatcataatg attaccacta gttcattgga
      601 tttcgattca ctaaagtcca agataaccag ttatttacga caaaccgcaa taaaatgaac
      661 gaattaaaaa ctttatttct caat
//
LOCUS       DAZKOB010000269          680 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000269 DAZKOB010000000
VERSION     DAZKOB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.281"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..447)
                     /locus_tag="ACG8M6_003090"
     CDS             complement(<1..447)
                     /locus_tag="ACG8M6_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458650.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          203 a          119 c           92 g          266 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg
//
LOCUS       DAZKOB010000270          670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000270 DAZKOB010000000
VERSION     DAZKOB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.282"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..417
                     /locus_tag="ACG8M6_003091"
     CDS             154..417
                     /locus_tag="ACG8M6_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0458651.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="ACG8M6_003092"
     CDS             417..>670
                     /locus_tag="ACG8M6_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0458652.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DAZKOB010000271          665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000271 DAZKOB010000000
VERSION     DAZKOB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.283"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          224 a          114 c           87 g          240 t
ORIGIN      
        1 actattcatt gatcataacg attggctatt tgattgtatc aaatctagga aatatggtat
       61 ttggtatcag taaagaattc agttggacta ctacattatg ggaatctcta ttctttttca
      121 tcttcgtatt tttgttacaa aattatcgaa aaaaataaac atgtgccatc tatctgttgg
      181 cacatgtttt aggatttatg gtacttttta ttcggcaatc cattttattt ttctacttct
      241 tcttaatacc tctaacactt ttgatttata aactaatcca atgcatctac ttgcgcttat
      301 taagctattt cagctagata gtaccacccc atgcgtcaat gcttgatttt ccttttgtat
      361 aacattgtat aagcaataaa ggacaaataa gtggtgatta caccaaacca ttgaataaga
      421 aaaattctga tacgtaaata aacatgaact tgaaaaaatt aatgaatcgg ttagctacta
      481 cgtagttgtc ttttttctca tacagttctg ttaacttaca cttctcttcg ctagataata
      541 aaaaatcctc caactgtatt gtccaatata atcccaggca tctaaactag cattatagaa
      601 gagacacaca tagaatctaa aataactggt tttaaatgtt tcttacaatc aaaaaaagct
      661 gttaa
//
LOCUS       DAZKOB010000272          656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000272 DAZKOB010000000
VERSION     DAZKOB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.284"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8M6_003093"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG8M6_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0458653.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAZKOB010000273          644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000273 DAZKOB010000000
VERSION     DAZKOB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.285"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            219..617
                     /gene="spxA"
                     /locus_tag="ACG8M6_003094"
     CDS             219..617
                     /gene="spxA"
                     /locus_tag="ACG8M6_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0458654.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          230 a           96 c          122 g          196 t
ORIGIN      
        1 tgaattagat ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta
       61 ctgtatatta accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct
      121 atttaaaagt tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac
      181 aaaaaagtga ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc
      241 caagttgtac ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg
      301 ttgaacgtaa tattttttca gaaccattga acagttctga attaaaagcg attttacaaa
      361 tgacagaaga cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga
      421 acatggattt agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg
      481 gattgcttcg ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag
      541 atgaaattcg tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat
      601 tgatggctgg attatagaag gataatacga aacttggaaa agac
//
LOCUS       DAZKOB010000274          583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000274 DAZKOB010000000
VERSION     DAZKOB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.286"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..>583)
                     /locus_tag="ACG8M6_003095"
     CDS             complement(38..>583)
                     /locus_tag="ACG8M6_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0458655.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          167 a          110 c          100 g          206 t
ORIGIN      
        1 aaatccactc cttttttcat gctctaaaat gatattttta cacaactgtt acggttgttt
       61 tacgaaattg gacgggactt tgccagttta atttctgttt tatccgttca ttattgtagt
      121 aatagatata gctatcgatg gctgatttta attcctcgaa acagttataa actttcccgt
      181 gaaaaatctc ttgttttaat aagccaaaaa aattctccat aagtgaatta tctaagcagt
      241 tccctttccg agacatgctt tgaaagattt tgtactcctt caatttgttt ctataagctt
      301 tcatttgata cgcccaacct tgatctgagt gaaacgtacg acgataggga caatcgttag
      361 tggtctggat agcttcctcc aaaccttcca ttaccgctaa tgcattgggc ttttcagaga
      421 tacgatagga taaaatctca ctattgaaca tatccataaa aggatctaga tacaatttct
      481 tttgacggat aatgcctttg gcatccactt catggtattt aaattcagtt gtatccgtcg
      541 tgattttctg atggcagata ctggtgtaga aacgacgatg gat
//
LOCUS       DAZKOB010000275          554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000275 DAZKOB010000000
VERSION     DAZKOB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.288"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8M6_003096"
     CDS             168..362
                     /locus_tag="ACG8M6_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458656.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          235 a           69 c           88 g          162 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat actt
//
LOCUS       DAZKOB010000276          543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000276 DAZKOB010000000
VERSION     DAZKOB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.289"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..492
                     /locus_tag="ACG8M6_003097"
     CDS             22..492
                     /locus_tag="ACG8M6_003097"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0458657.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          198 a           99 c           81 g          165 t
ORIGIN      
        1 aaatagaaag gagaaaactt tatgtctatt tataattttg aaactacttt agaagacggc
       61 actgtctatt cattgaaaaa atatgcaggc aaaccgataa tcattgttaa cactgcaaca
      121 aaatgcggtc tagcacctca atttaaacaa ttagaagaac tttaccaagc ttatcagaca
      181 caaggtctgg ttatcttagg ctttccttcc aatcaattta aacaagagct agattcagcg
      241 actgaagcag cagaagcttg taaaaccact tatggcgtta ccttccctat gcatcaattg
      301 attgacgtaa atggagaaaa tacagatcct ctttttactt atcttaaaaa tgaagctcct
      361 ggtactgtca ctaaagcaat aaaatggaat tttactaaat ttttgattga tagaaatggc
      421 aaagtaatcg aacgatttgc accacaaact tctccattga aaatgaccgc tgcaattgaa
      481 tccgttcttt aacttaataa aaataaaaaa agatgaacta tttctagttc atctttttgc
      541 ctt
//
LOCUS       DAZKOB010000277          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000277 DAZKOB010000000
VERSION     DAZKOB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.290"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..354
                     /locus_tag="ACG8M6_003098"
     CDS             <1..354
                     /locus_tag="ACG8M6_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0458658.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAZKOB010000278          519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000278 DAZKOB010000000
VERSION     DAZKOB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.291"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..438
                     /locus_tag="ACG8M6_003099"
     CDS             139..438
                     /locus_tag="ACG8M6_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0458659.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          199 a           63 c          102 g          155 t
ORIGIN      
        1 ttaaaataaa aatcgttgcc ataacagtgt attgaaaggt tttaacaatc cagtaatgat
       61 ttaaaaaaat ctagatgtag cgtatacttt gtttatagaa tgtagtggga agaaatatat
      121 ttttgggagg aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt actaaaatta
      181 tatgatctcg ttttaaatcc agagatcaaa gaaaaggaac gtatactttt gatagacgca
      241 aagacaggct tagaaaaagg tcagtattat cctaaagtca taaataattt ggaacgtagt
      301 ttacgtccgt tagcaatcag gggagaattg tctaaaccag tatcaccgtt ttatatggag
      361 atatcaacaa taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt
      421 acttttgggc atttataaaa acgagttatt catctcaatg aattaaaaag tatagtcaat
      481 ctcataatgc aggctaggtt catttatttg aacagagtc
//
LOCUS       DAZKOB010000279          517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000279 DAZKOB010000000
VERSION     DAZKOB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.292"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..>517
                     /locus_tag="ACG8M6_003100"
     CDS             57..>517
                     /locus_tag="ACG8M6_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0458660.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DAZKOB010000280          512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000280 DAZKOB010000000
VERSION     DAZKOB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.293"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..182
                     /locus_tag="ACG8M6_003101"
     CDS             60..182
                     /locus_tag="ACG8M6_003101"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458661.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            155..286
                     /locus_tag="ACG8M6_003102"
     CDS             155..286
                     /locus_tag="ACG8M6_003102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458662.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          190 a           78 c           91 g          153 t
ORIGIN      
        1 actagcacca cgggttaaat ctatattttg atatacaaat tacagcttca gatactaagt
       61 tgcataaacc ttttccggac cccatacaaa aggcaattga tgaattgggc ataaaaaata
      121 agaaacgatt tatattggtg attcattata tgacatgcaa agtgcgaaag cttgtgggat
      181 agcatttggt ttagctaaat ggggagccaa aaatatagaa aattttcaaa gtgtagatat
      241 aattattgat tctcctcaag aaatacttga tttgttagaa aaatagacaa agagcgtgag
      301 ataaggatag ggtctcacgc tctttgtttt tcgccattta aagattgaaa tttttgccat
      361 cgccgattgt aaaattaagc tagacaacta aaaaagtcat ttgtgctaca ctcaaaatag
      421 ttccgaccaa agaattataa ggagtgagta caaatgacct atacccatct tacaacggat
      481 gaacttgtaa taatagagtc ttatttcaaa at
//
LOCUS       DAZKOB010000281          512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000281 DAZKOB010000000
VERSION     DAZKOB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.294"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..343
                     /locus_tag="ACG8M6_003103"
     CDS             <1..343
                     /locus_tag="ACG8M6_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458663.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           69 c           93 g          144 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata at
//
LOCUS       DAZKOB010000282          495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000282 DAZKOB010000000
VERSION     DAZKOB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.295"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>495
                     /locus_tag="ACG8M6_003104"
     CDS             <1..>495
                     /locus_tag="ACG8M6_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0458664.1"
                     /translation="NDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYE
                     KYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETT
                     IIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIF
                     MDAT"
BASE COUNT          180 a           89 c           82 g          144 t
ORIGIN      
        1 gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcgatt taaatgaatt
       61 attccgttcg cacttagaaa aagcgataaa cacactccta cggactgaat taacggcttt
      121 tttagattac gaaaaatatg atcgcactgg ttttaattca ggtaattcga gaaacggttc
      181 ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca ttggaaatac ctagagatcg
      241 taatggtgag tttaaacaac aaactttacc agcctacaaa agaacaaacg atacattgga
      301 aaccactatt atccatttat tcgaaaaagg tgttacgatg tctgaaattg ctgatttgat
      361 cgaaaaaatg tacggtcatc actatactcc acaaaccatg tccaacatga ctaaagttct
      421 gactgaagaa gtaaatgcct ttaaatccag agccttaaat gataagtatg tcgctatttt
      481 tatggacgct actta
//
LOCUS       DAZKOB010000283          494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000283 DAZKOB010000000
VERSION     DAZKOB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.296"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..323
                     /locus_tag="ACG8M6_003105"
     CDS             9..323
                     /locus_tag="ACG8M6_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458665.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          164 a           58 c           76 g          196 t
ORIGIN      
        1 gatagctttt ggatttttac agtaatttta tcttaataat agcaatatta ttacttctaa
       61 atatttggtt ttttgataag agtagaaatg cagggatagg ttttagaacc aaacgaagta
      121 cgtcttctga aaaaaagtgg gtatattcac agactatttt ttacggaggg gttatttcaa
      181 tcagtttact ttcttcaaca ctttattctt ttaatgttat agacgtttcc atgtcaaatt
      241 ttatatctat tattggaatc cttatttctg caattattac acaactactt cttgtgtttg
      301 aagagaagtc aaaaaataat taaagcaatt atgcctttta gtacaggaat tattacttct
      361 tacaaatgta tagcgaatgt gaagcctgct gttttaattg ttctagaaaa acttttgagt
      421 gaacaattat tttatgttag gatgaattaa tttctctaga gtttataatt aaaaaactta
      481 tttgaaggaa catg
//
LOCUS       DAZKOB010000284          489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000284 DAZKOB010000000
VERSION     DAZKOB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.297"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            149..388
                     /locus_tag="ACG8M6_003106"
     CDS             149..388
                     /locus_tag="ACG8M6_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0458666.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT          204 a           58 c           93 g          134 t
ORIGIN      
        1 tctttctcaa aataaacata aaagcccttt aaagagggct tttatatatt aatcacaaat
       61 cacttatcac aaatcactta tcacaaatca caagtgattt gtgattgttg atgataaaat
      121 aagaataaga agaaatagaa agaagtgagt gattgtggga aatttaggcg cacaaaaaga
      181 aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata atgggagata agacggttcg
      241 tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt
      301 aaaagaagtt atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcatta
      361 tcttaaaatt ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa
      421 gaaggaggga ttcgtcatgt tggtattcca aatgcgtaat gtagataaaa catctactgt
      481 tttgaaaca
//
LOCUS       DAZKOB010000285          468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000285 DAZKOB010000000
VERSION     DAZKOB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.298"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACG8M6_003107"
     CDS             <1..>468
                     /locus_tag="ACG8M6_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0458667.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZKOB010000286          467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000286 DAZKOB010000000
VERSION     DAZKOB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.299"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..117)
                     /locus_tag="ACG8M6_003108"
     tRNA            complement(44..117)
                     /locus_tag="ACG8M6_003108"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(81..83),aa:Ile,seq:cat)
     gene            complement(284..>467)
                     /locus_tag="ACG8M6_003109"
     CDS             complement(284..>467)
                     /locus_tag="ACG8M6_003109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458668.1"
                     /translation="IKVHLKKSVRTKVLFVNLKIDFPEYVLGISKCLNVFLNAKSMQK
                     ILYFSTFLSLTKIKKR"
BASE COUNT          159 a           77 c           88 g          143 t
ORIGIN      
        1 ttcaaaaaat tctaacacag aacgattcaa ttggctacat taatgtaccc tgtaggactc
       61 gaacctacga ccggacggtt atgagccgtc tgctctgacc aactgagcta agggtagtgg
      121 ttgttgccac ataaagccat aaacaatcaa ccagtagaat gcgtggcaac aaacctgtta
      181 tcgcatatct tggagtgtga ctatttatga gtgatagtga agatatgcgt ctccatatat
      241 gtatggttga gacagataaa catgtatgaa aaacgttgtt aaatcaacgt ttttttattt
      301 tggtcaagga gagaaaagta gagaaataca aaattttttg cattgatttt gcattcaaaa
      361 aaacattcaa acatttagaa atccccaata cgtattcagg aaaatctatt ttaaggttta
      421 caaaaagaac ttttgttcgt acactctttt tgaggtgaac ttttatg
//
LOCUS       DAZKOB010000287          465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000287 DAZKOB010000000
VERSION     DAZKOB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.300"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..161
                     /locus_tag="ACG8M6_003110"
     CDS             <1..161
                     /locus_tag="ACG8M6_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0458669.1"
                     /translation="SFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANE
                     VLKIAEEK"
     gene            162..>465
                     /locus_tag="ACG8M6_003111"
     CDS             162..>465
                     /locus_tag="ACG8M6_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0458670.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERE"
BASE COUNT          180 a           55 c           96 g          134 t
ORIGIN      
        1 taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat atgtatgaga
       61 aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg aatgatttat
      121 atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt ttatttacta
      181 tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat ttggcaggaa
      241 agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa catcctgaac
      301 ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg acgactaagt
      361 taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat actgtaggga
      421 aaattgaagt gatacgtgat tcattcttac aactagaaag agaag
//
LOCUS       DAZKOB010000288          453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000288 DAZKOB010000000
VERSION     DAZKOB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.301"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..167
                     /locus_tag="ACG8M6_003112"
     CDS             <1..167
                     /locus_tag="ACG8M6_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HFQ0458671.1"
                     /translation="SQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFIL
                     KAVTGLNIET"
     gene            279..>453
                     /locus_tag="ACG8M6_003113"
     CDS             279..>453
                     /locus_tag="ACG8M6_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0458672.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELL"
BASE COUNT          186 a           65 c           64 g          138 t
ORIGIN      
        1 gtagtcaaga agatttatct ggactattat caatgttttc tcttgctata gaagaacaaa
       61 ttacattaga caaattgaaa atgttagatt atttatttca tcctagtttt agccaaccct
      121 ataattttat tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg
      181 aaatcaagaa atatactgta ttactcaatt cattgaaaat atttatcata atttttaata
      241 gaaaacacta ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg
      301 catcagcagt tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa
      361 gcgaaaattc aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt
      421 ccgatgtaac agcagagagt agtgaacttt tga
//
LOCUS       DAZKOB010000289          449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000289 DAZKOB010000000
VERSION     DAZKOB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.302"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..270)
                     /locus_tag="ACG8M6_003114"
                     /pseudo
     CDS             complement(34..270)
                     /locus_tag="ACG8M6_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          146 a           76 c           77 g          150 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaataacgc cgtttgtata attaagcgag acaaataaaa
      301 aagccattta tgttacactc caattgtaat acccgctaaa gcatacaagg agagtgaaca
      361 taaatgactt atacccatct tacaccaaac gagcttgtaa tgatagaagc atattttcat
      421 caagaaactc cggttgctat cgttgcgaa
//
LOCUS       DAZKOB010000290          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000290 DAZKOB010000000
VERSION     DAZKOB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.304"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG8M6_003115"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG8M6_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKOB010000291          420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000291 DAZKOB010000000
VERSION     DAZKOB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.305"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..403
                     /locus_tag="ACG8M6_003116"
     CDS             74..403
                     /locus_tag="ACG8M6_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0458673.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DAZKOB010000292          417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000292 DAZKOB010000000
VERSION     DAZKOB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.306"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..415)
                     /locus_tag="ACG8M6_003117"
     CDS             complement(<1..415)
                     /locus_tag="ACG8M6_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458674.1"
                     /translation="MICLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIF
                     SVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLS
                     LIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI"
BASE COUNT          178 a           49 c           65 g          125 t
ORIGIN      
        1 cgatgtagaa aataatgcct tccaatatac ctataaaata agtccagtat tccaaaggat
       61 tatataaata agtgacgtat ctatctaaag gtgtaataaa aatgataagt gataataaaa
      121 aaataccatt tataactaaa cataaactaa tggatttggg cggtttcagg ctcatcagaa
      181 aaatatagat tctggtgaac aacaaccata aaaaagtgag aatcattgca tgagagcttc
      241 tttccataaa ttttccttta agagtagtgc ttaaaagtac agaaaaaata ataaatgcaa
      301 ggataggagc aattaaagta aatttattta ataataaaca aaatttttca gcttttttat
      361 tcttggaata acgaggtatt gttttcgata aaaaaagatg gtgcagacaa attatga
//
LOCUS       DAZKOB010000293          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000293 DAZKOB010000000
VERSION     DAZKOB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.307"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>415
                     /locus_tag="ACG8M6_003118"
     CDS             <1..>415
                     /locus_tag="ACG8M6_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0458675.1"
                     /translation="LKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGII
                     LDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQG
                     LGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          154 a           55 c           75 g          131 t
ORIGIN      
        1 atttgaaaag tttgttgata gcgaagctat accaagcaaa taacaataag atttgttgta
       61 aattcgaatg tccaagtact attattgaag ttcctgttcc aatatttgac tgtattaggg
      121 tgcttggaat tattctcgat aatgcaattg aagcatctat agagagtcaa gatagagaac
      181 tgtctctagc tatttaccga gatgataaac agatagaaat ttcgatatcg aatagttgta
      241 gagagatact actctctata gatactctaa tgaaaaaagg aataacaaca aaaaagggac
      301 atcaagggtt aggtttatct agtatagagg aaatcaataa aaagaatccc aatatgtttg
      361 tgaactatca aaacgatccg tatcaattta ctactcaagt tattttgttg ttttg
//
LOCUS       DAZKOB010000294          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000294 DAZKOB010000000
VERSION     DAZKOB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.308"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG8M6_003119"
     rRNA            <1..>411
                     /locus_tag="ACG8M6_003119"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKOB010000295          389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000295 DAZKOB010000000
VERSION     DAZKOB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.309"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          171 a           52 c           65 g          101 t
ORIGIN      
        1 ttaagtagca aagcaataaa atagtatttt aaaaaacata taaaatagtt gttgacatgt
       61 tagaaatgac atgataagat aactgagttg ttaaaaaaga accacttgtg aagcttgatt
      121 ctatcgaaaa aaacttcaaa aaagttcttg acaaataaca aatgataagt tatgataaat
      181 gagttgctgt aagatttcga aaaacgaaat caaaaaaact tttaaaaaag agttgacaat
      241 tacttgtctc tctgatatga tataaaagtt gctacggcag ccaaaaacga atcggggaac
      301 actcctgatg aagtagacct ttgaaaactg aacaaagtaa gacaaaccaa atgtgtaggg
      361 cgtcttgatt caattcaaga caacaaaca
//
LOCUS       DAZKOB010000296          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000296 DAZKOB010000000
VERSION     DAZKOB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.310"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8M6_003120"
     CDS             <1..>386
                     /locus_tag="ACG8M6_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0458676.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKOB010000297          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000297 DAZKOB010000000
VERSION     DAZKOB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.311"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG8M6_003121"
     CDS             <1..>386
                     /locus_tag="ACG8M6_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0458677.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKOB010000298          380 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000298 DAZKOB010000000
VERSION     DAZKOB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.312"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..352
                     /locus_tag="ACG8M6_003122"
     CDS             <1..352
                     /locus_tag="ACG8M6_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFQ0458678.1"
                     /translation="RFYNRVIGIGNIIKIVLSASIPILIDQASEHRSLLLIVSIASAI
                     ITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITKTSPYNKTDEENFHLIVTTLQT
                     ELNDIISDFNQVNN"
BASE COUNT          152 a           50 c           51 g          127 t
ORIGIN      
        1 tcgattttat aatagagtaa ttggtatcgg aaatattata aaaatagtct tatcagctag
       61 tatcccaata ttaattgatc aggcttccga acacaggtct ttactattga ttgtttcaat
      121 tgcttcagca ataattacaa ttatacaaag tgggatgtct gcatttaatt atcaagataa
      181 agtacagact tcgacagagt tattaatgaa aattgaaaaa gaaaagttgt tatatataac
      241 gaaaacatcc ccatataata aaacagatga agaaaatttc catttaatcg ttacaaccct
      301 tcaaactgaa ttaaatgata ttatctctga ttttaatcaa gttaataact aaaaatttat
      361 ttaattagaa agtagtgcag
//
LOCUS       DAZKOB010000299          377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000299 DAZKOB010000000
VERSION     DAZKOB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.313"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          143 a           51 c           47 g          136 t
ORIGIN      
        1 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
       61 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
      121 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
      181 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
      241 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
      301 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
      361 gtactttatt taattta
//
LOCUS       DAZKOB010000300          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000300 DAZKOB010000000
VERSION     DAZKOB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.314"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG8M6_003123"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG8M6_003123"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKOB010000301          351 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000301 DAZKOB010000000
VERSION     DAZKOB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.316"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..>351
                     /locus_tag="ACG8M6_003124"
     CDS             11..>351
                     /locus_tag="ACG8M6_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458679.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFS"
BASE COUNT          114 a           52 c           43 g          142 t
ORIGIN      
        1 ggagaaaaaa atgaaaaaaa acaaaaacca aataatagat atcattttta tgttatttct
       61 aagttttgct tacgtcatcc ctctattcat gtctaaggga atctatcata gtgtcaatca
      121 ggatacttac tttcatttat ctcgaatcat aggactagat aatgtatgga gttctccagt
      181 aaacttcaat aacttcgatc atcatggaac aatgatgaat attttttatc catggcttac
      241 tctttatcca gcttttttat tttataaaat gatgggaaat cttgttttaa gttataatat
      301 ctactatttt tttatcactt ttttaacaat ggtcgtttct tatttttcta t
//
LOCUS       DAZKOB010000302          344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000302 DAZKOB010000000
VERSION     DAZKOB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.317"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>344
                     /locus_tag="ACG8M6_003125"
     CDS             <1..>344
                     /locus_tag="ACG8M6_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFQ0458680.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT          132 a           54 c           77 g           81 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caat
//
LOCUS       DAZKOB010000303          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000303 DAZKOB010000000
VERSION     DAZKOB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.318"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>341
                     /locus_tag="ACG8M6_003126"
     CDS             <1..>341
                     /locus_tag="ACG8M6_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0458681.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAZKOB010000304          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000304 DAZKOB010000000
VERSION     DAZKOB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.319"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACG8M6_003127"
     CDS             <1..>321
                     /locus_tag="ACG8M6_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFQ0458682.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DAZKOB010000305          300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000305 DAZKOB010000000
VERSION     DAZKOB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.320"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..>300)
                     /locus_tag="ACG8M6_003128"
     CDS             complement(48..>300)
                     /locus_tag="ACG8M6_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0458683.1"
                     /translation="RFVKDFYKVWDSEVFFLAIEDSLLINLYYSNKNYFKIPATKTRM
                     KKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT           95 a           59 c           45 g          101 t
ORIGIN      
        1 tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta atctttatac
       61 ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc gctccgtgcg
      121 tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat tctggttttt
      181 gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa taagctatct
      241 tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta
//
LOCUS       DAZKOB010000306          299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000306 DAZKOB010000000
VERSION     DAZKOB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.321"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..263
                     /locus_tag="ACG8M6_003129"
     CDS             63..263
                     /locus_tag="ACG8M6_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0458684.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          106 a           48 c           59 g           86 t
ORIGIN      
        1 cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat
       61 ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg
      121 gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa
      181 ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag
      241 caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaa
//
LOCUS       DAZKOB010000307          290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000307 DAZKOB010000000
VERSION     DAZKOB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.322"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          118 a           41 c           46 g           85 t
ORIGIN      
        1 ttcaaatata aaagagttta aagcgtaata cacttatggg gaagtggttt ggggtgcgct
       61 ttaaactctt ctttattatt atctcacagc atgcttaaaa ggtctatgga ttaagttatg
      121 taaaaaagtg cctctttaaa cgtcctaaaa tccaaacaag gatagcataa tatacactat
      181 cgttgatcca gaaaacaggt aaataaattt tgcaaaagca ataagttaaa aacattacaa
      241 aaaagacaag gatatcacat caagaaaaaa tgttttgaaa atatctttgc
//
LOCUS       DAZKOB010000308          287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000308 DAZKOB010000000
VERSION     DAZKOB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.323"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..287
                     /locus_tag="ACG8M6_003130"
                     /pseudo
     CDS             1..287
                     /locus_tag="ACG8M6_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          112 a           58 c           56 g           61 t
ORIGIN      
        1 aaacaacaaa tcgtcgatct ctatctcgct ggtaagcctc gcgcagaaat tattcgagag
       61 tatgagctta cgccttcttc tttcgataaa tggatgaagc aagcacaatc aacgggctca
      121 ttcaaagaaa gagacaactt aacaccagaa caagcagaat tgatcgcact aagaaagaaa
      181 aataagcaac tcgaaatgga gaatgatatt ttaaagcaag cggcgctgat attcggacga
      241 aaagacaagt aattgatgcc aacaagcata aatattccat atcagcg
//
LOCUS       DAZKOB010000309          285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000309 DAZKOB010000000
VERSION     DAZKOB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.denovo.324"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>285)
                     /locus_tag="ACG8M6_003131"
     CDS             complement(<1..>285)
                     /locus_tag="ACG8M6_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458685.1"
                     /translation="VFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFS
                     LLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSL"
BASE COUNT          106 a           48 c           36 g           95 t
ORIGIN      
        1 ataaactaac tatacttgtt agaacaaaaa aacaggcgtt gacttgttct tgattagtta
       61 aaaatatcaa taaaggaata cttacccaat atatttttct atttatttct tctcctctta
      121 agagttgaaa aaacggataa aaagttagta aactaaatgc tactggccac tgatagttca
      181 aggtggttgc tctccaaccg gtttccccca ttactgtcaa tggtattaaa cagtatatag
      241 agcaaaccaa taataaattt ttaacattta ttttctcaaa aacat
//
LOCUS       DAZKOB010000310        10893 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.guided.091, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000310 DAZKOB010000000
VERSION     DAZKOB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.guided.091"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..782
                     /locus_tag="ACG8M6_003132"
     CDS             <1..782
                     /locus_tag="ACG8M6_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0458686.1"
                     /translation="SARQQLANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFK
                     AEHEAMLKQAQDEPAIDEIYHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGA
                     TNNGRKERAESYKQEFIKRDVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELL
                     REGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPF
                     KPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(932..1873)
                     /locus_tag="ACG8M6_003133"
     CDS             complement(932..1873)
                     /locus_tag="ACG8M6_003133"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0458687.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1999..3120
                     /locus_tag="ACG8M6_003134"
     CDS             1999..3120
                     /locus_tag="ACG8M6_003134"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0458688.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3378..4880
                     /gene="eat(A)"
                     /locus_tag="ACG8M6_003135"
     CDS             3378..4880
                     /gene="eat(A)"
                     /locus_tag="ACG8M6_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0458689.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4947..5474
                     /locus_tag="ACG8M6_003136"
     CDS             4947..5474
                     /locus_tag="ACG8M6_003136"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0458690.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5685..6746
                     /locus_tag="ACG8M6_003137"
     CDS             5685..6746
                     /locus_tag="ACG8M6_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0458691.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6749..7645
                     /gene="murQ"
                     /locus_tag="ACG8M6_003138"
     CDS             6749..7645
                     /gene="murQ"
                     /locus_tag="ACG8M6_003138"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0458692.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7659..9641
                     /locus_tag="ACG8M6_003139"
     CDS             7659..9641
                     /locus_tag="ACG8M6_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0458693.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9706..10152)
                     /gene="nrdI"
                     /locus_tag="ACG8M6_003140"
     CDS             complement(9706..10152)
                     /gene="nrdI"
                     /locus_tag="ACG8M6_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0458694.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10363..>10893
                     /locus_tag="ACG8M6_003141"
     CDS             10363..>10893
                     /locus_tag="ACG8M6_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0458695.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTF"
BASE COUNT         3510 a         1914 c         2386 g         3083 t
ORIGIN      
        1 aatcagctag acaacagttg gcaaattatc agctgatcgt ttttttagat tatggatttg
       61 atatggagat ggctcgacgc gtgcgcccat ttaccaatgc aaaaattgtg ttattttttt
      121 ggaatcactt caaagcagaa cacgaggcga tgctgaagca ggcacaagat gaaccggcaa
      181 tcgatgagat ttatcatttt gattttttgg aagcaaaaga acttgggctg aaacataata
      241 gttcatttta ttcgaaacat atgaattttt cttctagcga accaaagacg gatctatttt
      301 ttggtgcaac gaataatggg agaaaagaac gagcagaaag ctataagcag gaatttataa
      361 aaagagatgt tacgaccaac tatttcattc ttccacatcg agggaatgaa caacctggat
      421 atcttactta tgaagagtac ctgaaaaaaa caggggaatc gcgcgggatt ctggaactgt
      481 tgcgggaggg tcagcaagga gtgactttgc ggacatttga atccatgtat tttcaaaaga
      541 aactagtgac ggataatcaa gcgattgctc attatcattt ttatcgtcct gaaaatattt
      601 ttttgcttcg agaacgtgat ttagatgaat taccggaatt tctgcataca ccatttaagc
      661 caatcctatc agagctattg gaattttatg atgcagaaaa ttgggcaaaa cgatttatca
      721 ctaacgattc cactatttat gagcaatacg aatatgacct ctcctctgtt gaaaacaaat
      781 aaaaaaccta cattttgaag agattatgta ctgaaacaaa caagcacaat tcttatggca
      841 tcatgagagt tgtgttttct ttttgatctt ttattgggct taaaaaaaga gaaccagctg
      901 ctgattcagc tgattctcat cagagagcct tttattctgc gccaaggatt ccttcttttg
      961 catggactaa ttgggtcaaa aaggcacaat atggtgtagc aatatcatat ttttgtcctt
     1021 ttcgccaaat ggcaccattg atgtagtcga tttcagtcaa acgatggttt ttgatcaggt
     1081 cttggtacat agatgggtaa tgcaacccga tgttgtcagg atcataagta gaagcaatat
     1141 gttggtacac ttcttcttga tctagtatga ttccttcttt tgctgcgata gaagcaaatt
     1201 cagatacgat tgttcgaacc atgctctctg ccgctttttg cgcgcctagt tcagcaatat
     1261 tacaatccaa aatcgtgcat aaaccattca atgttccatt tacacaagct tttcgccaaa
     1321 tggaataacg aacgttatgg ctatagatcg gatttaaatt tgcttcttga aagacttcta
     1381 caactttttt tgtaaatgct tcgccttctg gttccaagtt ctctagttcg atttctccat
     1441 cacctaacaa tgtgactttt ccgggaccag ctagaccagc tgtccacatc gtgatcccaa
     1501 ataagatatt tttctttggc acatattttt ccagtacatc ttcatgacct aatccattta
     1561 gtaaacacaa aacataagta ttgtcagtaa taatggattg gatcgaacaa aacatatcat
     1621 ctaattgatt ggcttttgtt aatgctacga tcaagtccac gtgttcgttg ctttcgatga
     1681 tttcttctgg tgaatagatt ggcagttttg cgaccacttc tttaccgtta aagtcagcaa
     1741 tcaacccatt tgtacgaatg gcttcaatgt gagcaggcca acgatcgatc aagaggacct
     1801 catttccgct ttgatgtagc atcaatccga ttcggctgcc cattgctccg gccccagcta
     1861 ttgcgatttt catgtttttt ctctccttta ttcaatgagt tcccttttag tatatagaaa
     1921 aatggaagaa aatagcattg ttttgtttga aaaaaatgaa aaaaagtgtc ttacttaaga
     1981 agaaagtgag ggatacagat ggatattttg gcggcaattg attcatttaa aggaagtgct
     2041 tcttcatttg aactaaatca ggcagttcta aatggctggg aagatcgtaa aggaagaaaa
     2101 atcaatgtgc cgattgcaga tggcggagaa aatactgcag aagcaatcta ttacgcgctc
     2161 aggggaacat ggcgtaccat tgatgggatg gatctcttat tccgtcagcg aaaaatcagc
     2221 tatttattga caacttggca aggaaaaaac accgcagtca tcgaatctgc acaagcattg
     2281 gggttagatc tacttgataa accaacggat caaactatcc gacttgcttc cagttatgcc
     2341 ttaggagaac ttattaaaga tgcactgctt caagaagtag aacaaattat tgtcacttta
     2401 ggaggaagcg ggtgtagcga tggtggtcta ggattactcc aatccttggg tgcttcttta
     2461 gaagggtaca cagaaggaaa tcctttatta tctactaaaa aacttcattt tgatcgaaga
     2521 aacgttcttg tttctaaagt aaaattgatt atcgctgcag atgttaccaa tccatataca
     2581 ggtcaacaag gagctgcttg gcaatttgga agacaaaaag gaggaataga tacgacgcta
     2641 gctttcttag ataaacaagc aggaaagatt gctcagcaaa taaaagattt ctgggaggtg
     2701 gatttagatg atattccagg aagtggagca gcaggcggta ttggtggagc cctttttttg
     2761 ttgggagcga agatggtttc tggtttcgat ttagtcagcc aaatcactgg attggaaaaa
     2821 aagatcgaac aagcagatgt catcattaca ggagaagggc ggatcgatca gcaaacgatc
     2881 cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa aatatcgaaa accaataatc
     2941 gctatttgcg gaagtagaga ccaagagcta gaaggacttt ctgattttct cccaattgtc
     3001 ttaagtattc atttgggtcc aataggttta gaagaagcaa tggaacacag gcaaacatta
     3061 gaaaaaacaa aaatcttagg acagacactt agcagatttt tcaccatcct cccgaagtag
     3121 ttgacaaaat cattttcttt atgctatcgt aatttcaaat ataggacaaa gtgagggatc
     3181 cgtgcattga aaaattaagt cgattgatga aagctgcatt cagactgtac cttgtggaga
     3241 caaggaagca gtgtgcagat tttcgggatt ggcttggttt tttcgatgta tgggtctttt
     3301 tgtgtgtatc caacccttgc atcctcttta gccagttctg aaaataggac tggtcttttt
     3361 ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa tctgacattc ggctacgaca
     3421 gccaaggcac attattattt gaacaagcaa atctaaattt tgacacacaa tggaaactag
     3481 gacttatcgg acgaaacggt cgaggaaaga caactttact gaatattcta caaaacaaac
     3541 taccttatca agggcaagta atccatcagc aagaatttgc ctatttcccg caacagacaa
     3601 aagataaaga acgtttaacc tattacgtgt taaatgatat tacggatttt gagatatggg
     3661 aaatcgaaag agagctccaa ttgatgcaaa cagatcctga aatcttatgg agagaattca
     3721 gcacactatc ggggggagag aagacaaaag tcctactggc acttttattt gtggatgaca
     3781 ctcatttccc gttaatcgat gaaccaacga atcatttgga tatctctggt agaaaacaag
     3841 tagcggctta tttgaaaaag aaaaaacaag gcttcatcgt ggtcagccat gaccggggat
     3901 ttatcgatga agtagtggac catgttttag caatcgaaaa aagtcaactg gaactttatc
     3961 aagggaattt ctctatctat gaagaacaga aaaaacttcg tgatgaattt gaaatggctc
     4021 aaaatgaaaa attgaaaaaa gaagtcagta ggctaaagaa aacagcagct gaaaaagccg
     4081 aatggtctcg ttcccgagaa ggagataaaa caaagaaaca agtcggattc atcgatactg
     4141 aatctagacg agtgaataaa ggagcagtgg gtgctgatgc tgcacggacg atgaaacgat
     4201 ccaaagcaat cgtgaatcgg atggagaccc agatcagcga gaaagaaaaa ctattaaaag
     4261 atatcgaata tatcgattct ttgacgatga atagccaagc gtctcaccat aagcgacttt
     4321 taagcgtaga agatcttcaa ttagggtatg aaaatctgtt attcgagcca attcatttta
     4381 caatcgagcc tcatcagcgg gtggcgattt caggtcctaa cggtgcagga aagtcatcca
     4441 ttatccatta tcttctgggg gcattcaacg gcaaggttat aggagaaaaa taccagccaa
     4501 aacatctgag cattagttat gcaagccaaa attatgaaga caatcgagga acgttggcgg
     4561 aatttgcaga gaaaaaccaa gtagactacc aagcattttt gaacaacctc cgaaagcttg
     4621 ggatggaaag agatgttttt cataacaaga tcgagcagat gagtatgggc caacggaaaa
     4681 aagtggaatt ggctaaatct ttatcacagc cagctgaact atatacatgg gatgaaccat
     4741 tgaattattt ggatgtcttc aatcaagaac aattagaaca actgatcttg aacgtgaaac
     4801 ctgccatgtt actagtggaa catgatcaaa ccttcctgga taaagtatct actgagatta
     4861 tttctcttga gagaatctaa cccttgtaat ctcaagaaca acaaggtatg gttaagggga
     4921 actttgccaa cgaaggagag aatcacatgc tttatgtaac gagacacgga gaaacaactt
     4981 ggaacgcaca aggattagtt tgcggacggg cagatgtgcc gctgactgaa aaaggacaga
     5041 tgcaggcaca gaaactagca gaaaaggtag tagatttgcc tgttccaatc acgaaaatca
     5101 tccattcccc tcttcaacga gcaagagata cagcacaagc agttgctgat cgtctcagct
     5161 tgccgctgac cgttgatgaa cgattggtag aaatggattt tggggattat gatggactgc
     5221 caagcaaaga tgagaacttt caaaaagcac gtttagcgtt cgctgtacga ttcccaaatg
     5281 gggagtcagt cttagatgtg tatgcacgga tcgttccttt attgaaagaa tgcatcgaag
     5341 acgaagaaaa tgtctatctg ttagtttgcc ataatgcctt gataagagtg atcaatgctt
     5401 attttcatcc gatgcctaat gaggggttct ttacattcat ggtagataat acagaattgg
     5461 tttcttatga gtaattatgt ttttgtgtta tttaaataaa atatttcaag gcactgcttt
     5521 tgcttagcag tgcctttttt aggttatgac aattcttttg ataatataaa ctcaaccttt
     5581 agaaatgaaa tttaattatt ttaataaaag tattgaaatt ttgtttcttt agatatatac
     5641 tctagtcata gaaatgaaga cggatacaga aaaagaggga taatatgttt ggcttttcaa
     5701 tatttatgaa cgattctttg tcagaagaga agaaacagta tatccgccaa atggcatcca
     5761 atggctttgt cggtattttc acttccatgc atataccaga agatgatgtt tcggcatatc
     5821 aaaagcgatt gatcgaacta ggaagcgaag cgaaaaagaa taaactagaa ttgatggtcg
     5881 atatttcagg agaggcattg gaacgagcgg gtttttcatt agaagatcta acgcctttga
     5941 aagcaattgg tgtaacaggt ttaagaatgg attatcatat ttccaatcag cagattgccg
     6001 actggtcaca tcaattgaaa atcagtttga atgctagcac gatcacgcct aaagatatcg
     6061 atgaattgaa agaagcagaa gctgatttct cacagattga agcttggcat aattattatc
     6121 cacgtcccga aacagggctg gacaaagaat ggtatcagaa aaaaaatcaa tggctcaaaa
     6181 ttcaaggtct ccttgtccaa ggttttgtac caggagatac agagttgaga ggcccactat
     6241 atcaaggatt gccaactttg gaagaacatc gagggatcca tcctcttgca ggcgctctag
     6301 atctattagc aagtaacacg gatattgttt acatcggaga tgcaggtctg tcagaaaacg
     6361 ttcaggagca atttgcttct ttccaaaaag aacagacagt cttgctccat acggaacctg
     6421 tagatgaaga attttacgaa tatatattag ggaagcatac gaatcggcaa gacgatgcaa
     6481 gagatgtcat acgaagcgta gatgcacgtt ttcgagagat tccgcctatc ccagcaagaa
     6541 atacagctac gcgtatgaaa gggtctatta ctttagataa cgaaaagtat ttgcgctata
     6601 tgggtgagat ccaattaacc aaatatgatt tgccagcgga tgaaaaagtg aatgtagtag
     6661 ctaaagttat caaagaagag ctaccattga tcaaccaaat caaagctggt atgaactatc
     6721 aatttataag aaaagaagga agataaaaat gagcaaaatc aatttagaga acctaacaac
     6781 ggaacgcaga aaccaagaaa cttttggatt agatgagatg agtatttctc aagccgttca
     6841 actaatgaac aaagaagatc agaaagtggc cgctgcggtt gaaaaagaac tgacaagtat
     6901 cgaaccagtg attgctaaca cgattgagtc ctttaaaaaa ggtggacgtc tgatttatat
     6961 gggagccgga acaagtggaa gactaggtgt gttagatgct gctgaatgcg taccgacatt
     7021 cggtgtagaa ccggaaatgg tcataggtct aattgctggt ggagaagaag caatgactgt
     7081 agctgtcgaa ggggccgaag attcttatga gctagggcaa gaagatctaa tcaagctgaa
     7141 tttgacaaaa aatgatatag ttattggtat cgcagctagc ggtcgtacac catatgtgat
     7201 cggtggatta gattacgcaa aatcgatcgg tgctgtcaca ggaacgattt cttgcaataa
     7261 acaagccgaa atcagtaaac acgcggattt cccaatcgaa gtcgatgcag gtccagaatt
     7321 tttgacaggc tctactcgat tgaagtcagg aacagcacaa aaacttatct tgaatatgat
     7381 ttctactatc agtatgattg gtataggtaa agtattcaat aacttgatgg tcgatgtcaa
     7441 accaacaaat gaaaaactaa tcgaacgctc aaaacgaatc atcatgcaag cgacagaagc
     7501 tgattacgaa acagctgaaa agtactttga agaagcagat caaaatgtta agctggctat
     7561 cgtcatgatt ttgacaaata gtgataaaga ggaagctcaa gcaaaattag ctgaagcaaa
     7621 cggatttgta aaagggacct tatgaggggg aaaagaccat ggcaaaaaac gaactgactt
     7681 tggaagaact ggcaaaacaa atttataaag gggcaggcgg catgggcaat gtagaaagca
     7741 ttgtccactg catgactcgt gtgcgtatga agatcatcaa tgatgatctt gtaaatgtct
     7801 ctgaattaaa agcgattcca ggagtgcttg gtgtagtaga agatgaacag cttcaagtca
     7861 tcatcggacc tgggaaagtg aacaaagtag cacagcaaat ggttgatttg gcaggcgtga
     7921 aattaggaga aaaactttct tctgtaagta cagctacagc aaattcatca gcatctggaa
     7981 aagaccgtgt aaacgaacgg gcacaagaaa tgaaagcggc acaaaaatcg aaacaaaaac
     8041 catctaaaat caaatcggta ttaaaagaca tttctaatat ctttgtgcca atgattcctg
     8101 catttgtcgg tactgggatc gtggcaggga ttgccgcagt tttagcaaac ttagtcacag
     8161 ccggaacact tgatgcagca acatggcaac aatatatcga tgtgatgaac atcttgaaaa
     8221 acgggatgtt tagctaccta gttatctata caggtatcaa tgctgcacaa gtttttggtg
     8281 caacaccaac tcttggtgga gttatcgggg ccgttgtgat gctgacaggg atgaatccgg
     8341 aagcaccact gaaaaactta tttactggtg aagctcttgc agctggacaa ggtgggatcc
     8401 taggtgtcat cttcgctgtt tggctattgt caatcgttga gaaaaaactg cataaagtag
     8461 tacctgatgc ggtagatatc atcgttacac caactctttc attgattgcc atcggtctga
     8521 ttgaaatttt cttcatcatg cctttagcag gtttcatttc tgatggcatg gtcggtggga
     8581 tcaactgggt acttggcgtt ggtggagcat ttgccggctt cgttctaggc acattattct
     8641 tgccgatggt tatgttcgga ctgcatcaaa ttttgactcc aatccatgtg caaatgattg
     8701 atgaaactgg tcgtacgcta ttgcttccaa ttcttgcgat ggctggtgca ggacaagtgg
     8761 gtgcagcatt ggcattatgg attcgctgca aaaaagataa agaattaaca gaaatgatca
     8821 aaggagcttt accagtcggg atcctaggaa tcggcgaacc attgatttac ggagtaacat
     8881 tacctctagg acgcccattt attactgctt gtatcggtgg aggtatcggc ggggcagtga
     8941 tcggaatgat cggaaatgtc ggggctatcg ctattggacc ttcaggagcc gctttgatcc
     9001 cattgatctc tgacggtaaa tggttaggct acatccttgg attattggca gcttatgctg
     9061 gtggcttcgt tgctacattc ttcttcggta ttcctaaaga ccgtttagaa aaagaagaat
     9121 tagcgactga aacagccaca gtaaatgaag tgcctgaaaa agtagtacct caaacttcag
     9181 ctgaaagtgt gactatcctc tcatcagttg ctgacggaac agttgtttct ttagataaag
     9241 caagtgatcc agtttttgca caaaagatga tgggtgaagg ctactttgta gaaccagaaa
     9301 acggacatat ctattctcca gttactgggg tagtcagctc tgtcttccca acgaaacacg
     9361 ctattgggat cacaacgcca aatggtttag aaatcttgct tcatatggga atcaacacag
     9421 ttgacttagg cggtacgcca tttgatgtga aagtaacaga agggcagcaa gtgacacctg
     9481 atacgcttgt agcagaagct gacttagaag cgatcaaagc agcaggcaaa gaaacatcca
     9541 tgatcgtctt ggtaaccaat atggatcgtg taaaaaactt tgtcttggaa aaaacaggta
     9601 aagcaaaagc aaaagcacct gtgatggatg tcaaagctta atatgataga aacaatagaa
     9661 acgaaagaag aaactgaaat ctatttttca gcttcttctt ttttattatt tattgttttc
     9721 tgctaaaatc tgataaattc gtgtcacatc agatggtgtt ccccgtaatt cataatctgc
     9781 caagaaagga aagccaaatg tttgtgcata ttgtttggct gttagacaat actgattatt
     9841 gaagttttta tttccgctac cgactacacc aagacaaagt ttatgattgt cttcatgttc
     9901 cagatattca cgcatcgttt cggtcaagat ttctgtatca ccgttatcta aaccatttcc
     9961 gccgtctaaa taagtaggaa cgaaagtaaa aaatggttct gtttctttcg taaaatcact
    10021 attttcatgg atttctttta aagtaacttc aggattgaca ggatcttctg catgcatttt
    10081 ttcagcatat tctgctagat gtttggcaaa ggcacgcgta tttcctgaaa tcgatatata
    10141 taaaatgttc atggatcatc ctccaattct ttccttttag tatagcatct tttttaagtt
    10201 ttttggtggt gtaatttgga aagggactag gcgctttgct tgtataatga gaaaaaagcc
    10261 ggtagaatta tatttctatt aaagttttta ttaattaagt aaaaataaaa aaggtttgtt
    10321 atactttttg ataaaagcgg ttttattttg gaggatgctt acatggatac agagcaacag
    10381 aaaaaaggaa ttggtttaat tccacttgct gccttagtaa ttggttcagc gattggaggt
    10441 ggcgtatttg gtattatgac ggacatctcc ggatcggctg gcggcccagc tattttcagt
    10501 tgggtactcg taggaacagc aatgttgatg ctctccttgt cgatcaataa tatcaataaa
    10561 aaacgtccag atttaaaagg cgggatcttt agttacgcag aagaaggttt tggccgtttc
    10621 gctggtttta tcagcggttg gggttactgg ctcacatgct ggttaggcaa cgtagctttt
    10681 gcaacattgt tgatgagcgc attaggatat ttcttcccag tatttgaagg tgggcaaaat
    10741 ctggcatcga tcgtgatgag tacagtcttt ttatggggat atgcatggct agtgaatcgg
    10801 ggagtcgaaa atgctagttt cgtcaatgct atcgtcacat tctgtaaatt agtgccatta
    10861 tttttattta ttgtagtagc tatcagcacg ttt
//
LOCUS       DAZKOB010000311        10892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.guided.092, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000311 DAZKOB010000000
VERSION     DAZKOB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.guided.092"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..782
                     /locus_tag="ACG8M6_003142"
     CDS             <1..782
                     /locus_tag="ACG8M6_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0458696.1"
                     /translation="SARQQLANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFK
                     AEHEAMLKQAQDEPAIDEIYHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGA
                     TNNGRKERAESYKQEFIKRDVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELL
                     REGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPF
                     KPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLSSVENK"
     gene            complement(932..1873)
                     /locus_tag="ACG8M6_003143"
     CDS             complement(932..1873)
                     /locus_tag="ACG8M6_003143"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0458697.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1999..3120
                     /locus_tag="ACG8M6_003144"
     CDS             1999..3120
                     /locus_tag="ACG8M6_003144"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0458698.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3378..4879
                     /gene="eat(A)"
                     /locus_tag="ACG8M6_003145"
                     /pseudo
     CDS             3378..4879
                     /gene="eat(A)"
                     /locus_tag="ACG8M6_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
     gene            4946..5473
                     /locus_tag="ACG8M6_003146"
     CDS             4946..5473
                     /locus_tag="ACG8M6_003146"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0458699.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5684..6745
                     /locus_tag="ACG8M6_003147"
     CDS             5684..6745
                     /locus_tag="ACG8M6_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0458700.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6748..7644
                     /gene="murQ"
                     /locus_tag="ACG8M6_003148"
     CDS             6748..7644
                     /gene="murQ"
                     /locus_tag="ACG8M6_003148"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0458701.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7658..9640
                     /locus_tag="ACG8M6_003149"
     CDS             7658..9640
                     /locus_tag="ACG8M6_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0458702.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9705..10151)
                     /gene="nrdI"
                     /locus_tag="ACG8M6_003150"
     CDS             complement(9705..10151)
                     /gene="nrdI"
                     /locus_tag="ACG8M6_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0458703.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10362..>10892
                     /locus_tag="ACG8M6_003151"
     CDS             10362..>10892
                     /locus_tag="ACG8M6_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0458704.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTF"
BASE COUNT         3510 a         1914 c         2385 g         3083 t
ORIGIN      
        1 aatcagctag acaacagttg gcaaattatc agctgatcgt ttttttagat tatggatttg
       61 atatggagat ggctcgacgc gtgcgcccat ttaccaatgc aaaaattgtg ttattttttt
      121 ggaatcactt caaagcagaa cacgaggcga tgctgaagca ggcacaagat gaaccggcaa
      181 tcgatgagat ttatcatttt gattttttgg aagcaaaaga acttgggctg aaacataata
      241 gttcatttta ttcgaaacat atgaattttt cttctagcga accaaagacg gatctatttt
      301 ttggtgcaac gaataatggg agaaaagaac gagcagaaag ctataagcag gaatttataa
      361 aaagagatgt tacgaccaac tatttcattc ttccacatcg agggaatgaa caacctggat
      421 atcttactta tgaagagtac ctgaaaaaaa caggggaatc gcgcgggatt ctggaactgt
      481 tgcgggaggg tcagcaagga gtgactttgc ggacatttga atccatgtat tttcaaaaga
      541 aactagtgac ggataatcaa gcgattgctc attatcattt ttatcgtcct gaaaatattt
      601 ttttgcttcg agaacgtgat ttagatgaat taccggaatt tctgcataca ccatttaagc
      661 caatcctatc agagctattg gaattttatg atgcagaaaa ttgggcaaaa cgatttatca
      721 ctaacgattc cactatttat gagcaatacg aatatgacct ctcctctgtt gaaaacaaat
      781 aaaaaaccta cattttgaag agattatgta ctgaaacaaa caagcacaat tcttatggca
      841 tcatgagagt tgtgttttct ttttgatctt ttattgggct taaaaaaaga gaaccagctg
      901 ctgattcagc tgattctcat cagagagcct tttattctgc gccaaggatt ccttcttttg
      961 catggactaa ttgggtcaaa aaggcacaat atggtgtagc aatatcatat ttttgtcctt
     1021 ttcgccaaat ggcaccattg atgtagtcga tttcagtcaa acgatggttt ttgatcaggt
     1081 cttggtacat agatgggtaa tgcaacccga tgttgtcagg atcataagta gaagcaatat
     1141 gttggtacac ttcttcttga tctagtatga ttccttcttt tgctgcgata gaagcaaatt
     1201 cagatacgat tgttcgaacc atgctctctg ccgctttttg cgcgcctagt tcagcaatat
     1261 tacaatccaa aatcgtgcat aaaccattca atgttccatt tacacaagct tttcgccaaa
     1321 tggaataacg aacgttatgg ctatagatcg gatttaaatt tgcttcttga aagacttcta
     1381 caactttttt tgtaaatgct tcgccttctg gttccaagtt ctctagttcg atttctccat
     1441 cacctaacaa tgtgactttt ccgggaccag ctagaccagc tgtccacatc gtgatcccaa
     1501 ataagatatt tttctttggc acatattttt ccagtacatc ttcatgacct aatccattta
     1561 gtaaacacaa aacataagta ttgtcagtaa taatggattg gatcgaacaa aacatatcat
     1621 ctaattgatt ggcttttgtt aatgctacga tcaagtccac gtgttcgttg ctttcgatga
     1681 tttcttctgg tgaatagatt ggcagttttg cgaccacttc tttaccgtta aagtcagcaa
     1741 tcaacccatt tgtacgaatg gcttcaatgt gagcaggcca acgatcgatc aagaggacct
     1801 catttccgct ttgatgtagc atcaatccga ttcggctgcc cattgctccg gccccagcta
     1861 ttgcgatttt catgtttttt ctctccttta ttcaatgagt tcccttttag tatatagaaa
     1921 aatggaagaa aatagcattg ttttgtttga aaaaaatgaa aaaaagtgtc ttacttaaga
     1981 agaaagtgag ggatacagat ggatattttg gcggcaattg attcatttaa aggaagtgct
     2041 tcttcatttg aactaaatca ggcagttcta aatggctggg aagatcgtaa aggaagaaaa
     2101 atcaatgtgc cgattgcaga tggcggagaa aatactgcag aagcaatcta ttacgcgctc
     2161 aggggaacat ggcgtaccat tgatgggatg gatctcttat tccgtcagcg aaaaatcagc
     2221 tatttattga caacttggca aggaaaaaac accgcagtca tcgaatctgc acaagcattg
     2281 gggttagatc tacttgataa accaacggat caaactatcc gacttgcttc cagttatgcc
     2341 ttaggagaac ttattaaaga tgcactgctt caagaagtag aacaaattat tgtcacttta
     2401 ggaggaagcg ggtgtagcga tggtggtcta ggattactcc aatccttggg tgcttcttta
     2461 gaagggtaca cagaaggaaa tcctttatta tctactaaaa aacttcattt tgatcgaaga
     2521 aacgttcttg tttctaaagt aaaattgatt atcgctgcag atgttaccaa tccatataca
     2581 ggtcaacaag gagctgcttg gcaatttgga agacaaaaag gaggaataga tacgacgcta
     2641 gctttcttag ataaacaagc aggaaagatt gctcagcaaa taaaagattt ctgggaggtg
     2701 gatttagatg atattccagg aagtggagca gcaggcggta ttggtggagc cctttttttg
     2761 ttgggagcga agatggtttc tggtttcgat ttagtcagcc aaatcactgg attggaaaaa
     2821 aagatcgaac aagcagatgt catcattaca ggagaagggc ggatcgatca gcaaacgatc
     2881 cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa aatatcgaaa accaataatc
     2941 gctatttgcg gaagtagaga ccaagagcta gaaggacttt ctgattttct cccaattgtc
     3001 ttaagtattc atttgggtcc aataggttta gaagaagcaa tggaacacag gcaaacatta
     3061 gaaaaaacaa aaatcttagg acagacactt agcagatttt tcaccatcct cccgaagtag
     3121 ttgacaaaat cattttcttt atgctatcgt aatttcaaat ataggacaaa gtgagggatc
     3181 cgtgcattga aaaattaagt cgattgatga aagctgcatt cagactgtac cttgtggaga
     3241 caaggaagca gtgtgcagat tttcgggatt ggcttggttt tttcgatgta tgggtctttt
     3301 tgtgtgtatc caacccttgc atcctcttta gccagttctg aaaataggac tggtcttttt
     3361 ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa tctgacattc ggctacgaca
     3421 gccaaggcac attattattt gaacaagcaa atctaaattt tgacacacaa tggaaactag
     3481 gacttatcgg acgaaacggt cgaggaaaga caactttact gaatattcta caaaacaaac
     3541 taccttatca agggcaagta atccatcagc aagaatttgc ctatttcccg caacagacaa
     3601 aagataaaga acgtttaacc tattacgtgt taaatgatat tacggatttt gagatatggg
     3661 aaatcgaaag agagctccaa ttgatgcaaa cagatcctga aatcttatgg agagaattca
     3721 gcacactatc gggggagaga agacaaaagt cctactggca cttttatttg tggatgacac
     3781 tcatttcccg ttaatcgatg aaccaacgaa tcatttggat atctctggta gaaaacaagt
     3841 agcggcttat ttgaaaaaga aaaaacaagg cttcatcgtg gtcagccatg accggggatt
     3901 tatcgatgaa gtagtggacc atgttttagc aatcgaaaaa agtcaactgg aactttatca
     3961 agggaatttc tctatctatg aagaacagaa aaaacttcgt gatgaatttg aaatggctca
     4021 aaatgaaaaa ttgaaaaaag aagtcagtag gctaaagaaa acagcagctg aaaaagccga
     4081 atggtctcgt tcccgagaag gagataaaac aaagaaacaa gtcggattca tcgatactga
     4141 atctagacga gtgaataaag gagcagtggg tgctgatgct gcacggacga tgaaacgatc
     4201 caaagcaatc gtgaatcgga tggagaccca gatcagcgag aaagaaaaac tattaaaaga
     4261 tatcgaatat atcgattctt tgacgatgaa tagccaagcg tctcaccata agcgactttt
     4321 aagcgtagaa gatcttcaat tagggtatga aaatctgtta ttcgagccaa ttcattttac
     4381 aatcgagcct catcagcggg tggcgatttc aggtcctaac ggtgcaggaa agtcatccat
     4441 tatccattat cttctggggg cattcaacgg caaggttata ggagaaaaat accagccaaa
     4501 acatctgagc attagttatg caagccaaaa ttatgaagac aatcgaggaa cgttggcgga
     4561 atttgcagag aaaaaccaag tagactacca agcatttttg aacaacctcc gaaagcttgg
     4621 gatggaaaga gatgtttttc ataacaagat cgagcagatg agtatgggcc aacggaaaaa
     4681 agtggaattg gctaaatctt tatcacagcc agctgaacta tatacatggg atgaaccatt
     4741 gaattatttg gatgtcttca atcaagaaca attagaacaa ctgatcttga acgtgaaacc
     4801 tgccatgtta ctagtggaac atgatcaaac cttcctggat aaagtatcta ctgagattat
     4861 ttctcttgag agaatctaac ccttgtaatc tcaagaacaa caaggtatgg ttaaggggaa
     4921 ctttgccaac gaaggagaga atcacatgct ttatgtaacg agacacggag aaacaacttg
     4981 gaacgcacaa ggattagttt gcggacgggc agatgtgccg ctgactgaaa aaggacagat
     5041 gcaggcacag aaactagcag aaaaggtagt agatttgcct gttccaatca cgaaaatcat
     5101 ccattcccct cttcaacgag caagagatac agcacaagca gttgctgatc gtctcagctt
     5161 gccgctgacc gttgatgaac gattggtaga aatggatttt ggggattatg atggactgcc
     5221 aagcaaagat gagaactttc aaaaagcacg tttagcgttc gctgtacgat tcccaaatgg
     5281 ggagtcagtc ttagatgtgt atgcacggat cgttccttta ttgaaagaat gcatcgaaga
     5341 cgaagaaaat gtctatctgt tagtttgcca taatgccttg ataagagtga tcaatgctta
     5401 ttttcatccg atgcctaatg aggggttctt tacattcatg gtagataata cagaattggt
     5461 ttcttatgag taattatgtt tttgtgttat ttaaataaaa tatttcaagg cactgctttt
     5521 gcttagcagt gcctttttta ggttatgaca attcttttga taatataaac tcaaccttta
     5581 gaaatgaaat ttaattattt taataaaagt attgaaattt tgtttcttta gatatatact
     5641 ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg gcttttcaat
     5701 atttatgaac gattctttgt cagaagagaa gaaacagtat atccgccaaa tggcatccaa
     5761 tggctttgtc ggtattttca cttccatgca tataccagaa gatgatgttt cggcatatca
     5821 aaagcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagaat tgatggtcga
     5881 tatttcagga gaggcattgg aacgagcggg tttttcatta gaagatctaa cgcctttgaa
     5941 agcaattggt gtaacaggtt taagaatgga ttatcatatt tccaatcagc agattgccga
     6001 ctggtcacat caattgaaaa tcagtttgaa tgctagcacg atcacgccta aagatatcga
     6061 tgaattgaaa gaagcagaag ctgatttctc acagattgaa gcttggcata attattatcc
     6121 acgtcccgaa acagggctgg acaaagaatg gtatcagaaa aaaaatcaat ggctcaaaat
     6181 tcaaggtctc cttgtccaag gttttgtacc aggagataca gagttgagag gcccactata
     6241 tcaaggattg ccaactttgg aagaacatcg agggatccat cctcttgcag gcgctctaga
     6301 tctattagca agtaacacgg atattgttta catcggagat gcaggtctgt cagaaaacgt
     6361 tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata cggaacctgt
     6421 agatgaagaa ttttacgaat atatattagg gaagcatacg aatcggcaag acgatgcaag
     6481 agatgtcata cgaagcgtag atgcacgttt tcgagagatt ccgcctatcc cagcaagaaa
     6541 tacagctacg cgtatgaaag ggtctattac tttagataac gaaaagtatt tgcgctatat
     6601 gggtgagatc caattaacca aatatgattt gccagcggat gaaaaagtga atgtagtagc
     6661 taaagttatc aaagaagagc taccattgat caaccaaatc aaagctggta tgaactatca
     6721 atttataaga aaagaaggaa gataaaaatg agcaaaatca atttagagaa cctaacaacg
     6781 gaacgcagaa accaagaaac ttttggatta gatgagatga gtatttctca agccgttcaa
     6841 ctaatgaaca aagaagatca gaaagtggcc gctgcggttg aaaaagaact gacaagtatc
     6901 gaaccagtga ttgctaacac gattgagtcc tttaaaaaag gtggacgtct gatttatatg
     6961 ggagccggaa caagtggaag actaggtgtg ttagatgctg ctgaatgcgt accgacattc
     7021 ggtgtagaac cggaaatggt cataggtcta attgctggtg gagaagaagc aatgactgta
     7081 gctgtcgaag gggccgaaga ttcttatgag ctagggcaag aagatctaat caagctgaat
     7141 ttgacaaaaa atgatatagt tattggtatc gcagctagcg gtcgtacacc atatgtgatc
     7201 ggtggattag attacgcaaa atcgatcggt gctgtcacag gaacgatttc ttgcaataaa
     7261 caagccgaaa tcagtaaaca cgcggatttc ccaatcgaag tcgatgcagg tccagaattt
     7321 ttgacaggct ctactcgatt gaagtcagga acagcacaaa aacttatctt gaatatgatt
     7381 tctactatca gtatgattgg tataggtaaa gtattcaata acttgatggt cgatgtcaaa
     7441 ccaacaaatg aaaaactaat cgaacgctca aaacgaatca tcatgcaagc gacagaagct
     7501 gattacgaaa cagctgaaaa gtactttgaa gaagcagatc aaaatgttaa gctggctatc
     7561 gtcatgattt tgacaaatag tgataaagag gaagctcaag caaaattagc tgaagcaaac
     7621 ggatttgtaa aagggacctt atgaggggga aaagaccatg gcaaaaaacg aactgacttt
     7681 ggaagaactg gcaaaacaaa tttataaagg ggcaggcggc atgggcaatg tagaaagcat
     7741 tgtccactgc atgactcgtg tgcgtatgaa gatcatcaat gatgatcttg taaatgtctc
     7801 tgaattaaaa gcgattccag gagtgcttgg tgtagtagaa gatgaacagc ttcaagtcat
     7861 catcggacct gggaaagtga acaaagtagc acagcaaatg gttgatttgg caggcgtgaa
     7921 attaggagaa aaactttctt ctgtaagtac agctacagca aattcatcag catctggaaa
     7981 agaccgtgta aacgaacggg cacaagaaat gaaagcggca caaaaatcga aacaaaaacc
     8041 atctaaaatc aaatcggtat taaaagacat ttctaatatc tttgtgccaa tgattcctgc
     8101 atttgtcggt actgggatcg tggcagggat tgccgcagtt ttagcaaact tagtcacagc
     8161 cggaacactt gatgcagcaa catggcaaca atatatcgat gtgatgaaca tcttgaaaaa
     8221 cgggatgttt agctacctag ttatctatac aggtatcaat gctgcacaag tttttggtgc
     8281 aacaccaact cttggtggag ttatcggggc cgttgtgatg ctgacaggga tgaatccgga
     8341 agcaccactg aaaaacttat ttactggtga agctcttgca gctggacaag gtgggatcct
     8401 aggtgtcatc ttcgctgttt ggctattgtc aatcgttgag aaaaaactgc ataaagtagt
     8461 acctgatgcg gtagatatca tcgttacacc aactctttca ttgattgcca tcggtctgat
     8521 tgaaattttc ttcatcatgc ctttagcagg tttcatttct gatggcatgg tcggtgggat
     8581 caactgggta cttggcgttg gtggagcatt tgccggcttc gttctaggca cattattctt
     8641 gccgatggtt atgttcggac tgcatcaaat tttgactcca atccatgtgc aaatgattga
     8701 tgaaactggt cgtacgctat tgcttccaat tcttgcgatg gctggtgcag gacaagtggg
     8761 tgcagcattg gcattatgga ttcgctgcaa aaaagataaa gaattaacag aaatgatcaa
     8821 aggagcttta ccagtcggga tcctaggaat cggcgaacca ttgatttacg gagtaacatt
     8881 acctctagga cgcccattta ttactgcttg tatcggtgga ggtatcggcg gggcagtgat
     8941 cggaatgatc ggaaatgtcg gggctatcgc tattggacct tcaggagccg ctttgatccc
     9001 attgatctct gacggtaaat ggttaggcta catccttgga ttattggcag cttatgctgg
     9061 tggcttcgtt gctacattct tcttcggtat tcctaaagac cgtttagaaa aagaagaatt
     9121 agcgactgaa acagccacag taaatgaagt gcctgaaaaa gtagtacctc aaacttcagc
     9181 tgaaagtgtg actatcctct catcagttgc tgacggaaca gttgtttctt tagataaagc
     9241 aagtgatcca gtttttgcac aaaagatgat gggtgaaggc tactttgtag aaccagaaaa
     9301 cggacatatc tattctccag ttactggggt agtcagctct gtcttcccaa cgaaacacgc
     9361 tattgggatc acaacgccaa atggtttaga aatcttgctt catatgggaa tcaacacagt
     9421 tgacttaggc ggtacgccat ttgatgtgaa agtaacagaa gggcagcaag tgacacctga
     9481 tacgcttgta gcagaagctg acttagaagc gatcaaagca gcaggcaaag aaacatccat
     9541 gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa aaacaggtaa
     9601 agcaaaagca aaagcacctg tgatggatgt caaagcttaa tatgatagaa acaatagaaa
     9661 cgaaagaaga aactgaaatc tatttttcag cttcttcttt tttattattt attgttttct
     9721 gctaaaatct gataaattcg tgtcacatca gatggtgttc cccgtaattc ataatctgcc
     9781 aagaaaggaa agccaaatgt ttgtgcatat tgtttggctg ttagacaata ctgattattg
     9841 aagtttttat ttccgctacc gactacacca agacaaagtt tatgattgtc ttcatgttcc
     9901 agatattcac gcatcgtttc ggtcaagatt tctgtatcac cgttatctaa accatttccg
     9961 ccgtctaaat aagtaggaac gaaagtaaaa aatggttctg tttctttcgt aaaatcacta
    10021 ttttcatgga tttcttttaa agtaacttca ggattgacag gatcttctgc atgcattttt
    10081 tcagcatatt ctgctagatg tttggcaaag gcacgcgtat ttcctgaaat cgatatatat
    10141 aaaatgttca tggatcatcc tccaattctt tccttttagt atagcatctt ttttaagttt
    10201 tttggtggtg taatttggaa agggactagg cgctttgctt gtataatgag aaaaaagccg
    10261 gtagaattat atttctatta aagtttttat taattaagta aaaataaaaa aggtttgtta
    10321 tactttttga taaaagcggt tttattttgg aggatgctta catggataca gagcaacaga
    10381 aaaaaggaat tggtttaatt ccacttgctg ccttagtaat tggttcagcg attggaggtg
    10441 gcgtatttgg tattatgacg gacatctccg gatcggctgg cggcccagct attttcagtt
    10501 gggtactcgt aggaacagca atgttgatgc tctccttgtc gatcaataat atcaataaaa
    10561 aacgtccaga tttaaaaggc gggatcttta gttacgcaga agaaggtttt ggccgtttcg
    10621 ctggttttat cagcggttgg ggttactggc tcacatgctg gttaggcaac gtagcttttg
    10681 caacattgtt gatgagcgca ttaggatatt tcttcccagt atttgaaggt gggcaaaatc
    10741 tggcatcgat cgtgatgagt acagtctttt tatggggata tgcatggcta gtgaatcggg
    10801 gagtcgaaaa tgctagtttc gtcaatgcta tcgtcacatt ctgtaaatta gtgccattat
    10861 ttttatttat tgtagtagct atcagcacgt tt
//
LOCUS       DAZKOB010000312         5638 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.guided.141, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000312 DAZKOB010000000
VERSION     DAZKOB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.guided.141"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..801
                     /locus_tag="ACG8M6_003152"
     CDS             109..801
                     /locus_tag="ACG8M6_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0458705.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1128..1625
                     /gene="dfrG"
                     /locus_tag="ACG8M6_003153"
     CDS             1128..1625
                     /gene="dfrG"
                     /locus_tag="ACG8M6_003153"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0458706.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1697..3649)
                     /locus_tag="ACG8M6_003154"
     CDS             complement(1697..3649)
                     /locus_tag="ACG8M6_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0458707.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3656..3892)
                     /locus_tag="ACG8M6_003155"
     CDS             complement(3656..3892)
                     /locus_tag="ACG8M6_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458708.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4294..4584
                     /locus_tag="ACG8M6_003156"
     CDS             4294..4584
                     /locus_tag="ACG8M6_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458709.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(4856..5205)
                     /locus_tag="ACG8M6_003157"
                     /pseudo
     CDS             complement(4856..5205)
                     /locus_tag="ACG8M6_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            5298..5600
                     /locus_tag="ACG8M6_003158"
                     /pseudo
     CDS             5298..5600
                     /locus_tag="ACG8M6_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1914 a          905 c          980 g         1839 t
ORIGIN      
        1 tgatagtatt tatattaaaa ttacatatgt aatttaaaaa cgcttgacat tgtgtttgag
       61 atcacttact tttagcatgt aaaagtattg gattaaggag ctatgcaaat gaaaaagaaa
      121 tttattactg gtgggattgt cgttgcattt ctatgtctaa ttggctacac aggcacgcgg
      181 tacgcaaatg aaattatttt gtggggtgga gagacagata ttgatacaat caatgagaat
      241 ctgacacagc ttgatcatac attgacagat aaagagcaag cgattacaga tcttaccgct
      301 cgactagcac aaaaagaaca gacattgcaa gattcaaagg cagatgtcca agaataccaa
      361 acaaaagtaa atgaattaga aaaccaaaaa aatcaatcgg cagcagaaaa acaaaactta
      421 gaaagtcaat tagtcagcaa ggatgcacaa ctacaaagca aacaaaatga aatcaatgtc
      481 aaactagatg agatcaacca aaaaaatgat gaaatcaatc atctgaatgc agattggaca
      541 gcaagactac aagaagcaca agcaaaactt tcagaattac aaaatactgt aaatggtttg
      601 aatcaacaaa tcgcaacact gacacaagaa aaaacaaatg tagaaactca gttaacagat
      661 acacaaaaga aattagacga agcaactgct gaaaactcaa gcttgaaaca atatattcaa
      721 aagttagaaa aagcaaaaga tgaagtggaa gatacagctg ataaatctca gcaaattgtt
      781 gaagaacatt tgcgtaaata atctttctta acaagtaaac ctaaattagt aaagaagcaa
      841 cagtaatcta tactaagtag ttcagttttg gagtacaaaa tgacaaaatg tacttcaaaa
      901 tggactactt ttttcatttt aacttacata ccaaattaca tacaaaatga tttataaaat
      961 gcaattgaaa atgataattg aatgaaaatt tgtgattttg tggtattatg ataatataac
     1021 ttatagaaaa aattgcttat tcaactaacg ggcaggataa tgagaaaagg ttttttgata
     1081 gaaataagaa attagatggt aaattagtta aaaaggctgt gttataaatg aaagtttctt
     1141 tgattgctgc gatggataag aatagagtga ttggcaaaga gaatgacatt ccttggagga
     1201 ttcccaagga ctgggaatat gttaaaaata ctacaaaggg acatccgata atattaggta
     1261 ggaagaacct tgaatcaatc ggaagagcct tacctgacag aagaaatatt attctgacga
     1321 gagataaggg gtttaccttt aatggttgtg aaattgttca ttcaatagaa gatgtttttg
     1381 agttatgtaa aaacgaagaa gaaattttta ttttcggagg agaacagatt tataatttgt
     1441 ttttccctta tgttgagaaa atgtacatca caaaaataca tcatgaattc gaaggagata
     1501 ctttttttcc agaagtgaat tatgaggaat ggaatgaggt atttgcccaa aaagggataa
     1561 agaatgataa aaatccgtat aactactatt ttcatgtata tgaaagaaaa aacttattga
     1621 gttaacgggt gcgttaattg aagaagaaca gtaaagataa agcacaagag cttgggatta
     1681 ctccaagctc tttttgtcat ttgaaataaa ttatatcttt ccaatcaaat tgtttatctg
     1741 taatccctaa tctttgagca ggtgtcaaaa cctttttatc cgctgattta aaagggcgac
     1801 aaaagttgta gtaagttctt agaattgtaa tcgcaaattg agcatacttc ggattgaagt
     1861 tagcgtaaat atagctcttc ttatcaccac gagctgttgt taatggtctt tcaagagatg
     1921 atatataacg tcttatctgt tgaataaagc tattggtcga atggtcatta acatttaata
     1981 gcatctttgc gatttcgttt ggttccagag cagataaatc cgttctacaa tcaactgaat
     2041 aaaatccttt atccttagtg gctaatggat gtttgatagg gttatcggca tatttgcgat
     2101 atctttctcc atctttacta atagcctctt cgtggaattg atgacttgtt agtaattcag
     2161 ttagataatg agatgccaaa gtccgtagaa actttgtttt gaatcctctg atatctcccc
     2221 aatcgagcag ttctgctttt gcttgtccga attcttttaa gcattgtttt ctgcttttcg
     2281 tcttgttaac ttggcaaata aaatgatgtg catcgctcaa ccttaattct ttcgtgaata
     2341 ctctataaaa ggcgtttcta atagagaagt catcatcact aatcattcgc cattcgtcgg
     2401 cattaaccat ttgtttaatt aaccaatagt gagcagttgt agtataagga gcatcaacat
     2461 ttaaaccatc gacaaatcta ctcctattag taattttccc taattcgtga agataagcat
     2521 tcctattttc gctatcgtta gcggatggtt cttgtggata ataagaccaa tctaacctat
     2581 catttttacg actaaaagta ttgagatggt cttctttgaa ttttcttgta tcttcattta
     2641 attcatccat tgaaatattc caatcatagg caacatcaga acggaacaca tatcttgata
     2701 agacctcagc agatacaact atatacgttt gcatattaag gtcttcaaac cccgtatatt
     2761 tctttgaacc ttgtcctttc ttacggacgt tatttaggta gtagtgcatc ttgtccgtat
     2821 taagccacat ctcgttaaat ttctttgttt gaaggggttg tgtctcatat cgttctaaaa
     2881 actccaaaca acgcctatac agccactcca gcttatgata gtaggttcca actccaattc
     2941 ctaaaatatc acaagtacga ctgactgaaa cccttccaac aaccatctta gcgaacattg
     3001 gaagaatggt gttcttttgt tggtgataag tagtggtttc ttctcttttt ggtagaacat
     3061 tagtgaactt cttacacgcc ttacattgat agcgttgaga tttgccccta cttttccccc
     3121 ttttgtaaaa ttgcttcggc tcattgaatg gggttgattc ttcttccgag cagccttctt
     3181 tatgaaattg ataatctggc tcaacatctt ggatgctatt gatttctata aggcgtttaa
     3241 tttcttcaac aacagaccaa ttagataaag gggttactgt acagttttgc gatacacctc
     3301 tgccaatagg attgtcattg cagtacatac ctttatgacc tttatctttg gaactaccag
     3361 tcattttata tcgacttggt ttccctttta catctttgaa tttgtgctgt tctttcccaa
     3421 aattacaaca aaacggattg atgcagtaat tatattgaat gggatattca gaaccgttcc
     3481 atttaaaaga cgttggcaag aacaacattt tgttatacct tgttgcaaat cgttttggag
     3541 ttaataaatc atattgttta gccctatctt ttaattcttc gtttgaaatc ggaattttaa
     3601 catctacaat ttcatcttcc ttcgtggcta atcttttaag cttggtcatt gtctatcaac
     3661 ccactctatc tttgataccc attttttcaa caatccgctt attacttcgc ttgttggcaa
     3721 tccattcaat aaacttctta tcgtctaaaa tgttcaataa gaattcatcc actgcttcgc
     3781 tttcagtacg ctctgcatat tcagcgtatt gcttaactat cacccttact tgctctgata
     3841 tttcccaatc cacagactta gcgttcttat tttttggctc aagaaattgc ataggaaaat
     3901 gcaccccttt ttgcttagta aattgtaacc attttaacac accaatttgc attcctacaa
     3961 taattatttc acgttcgcat ttttatatac attttaactt acattttgtg gtgtaaatta
     4021 acaacaaacg ttgatttaac aataaaaaac gagtatcaaa ttcagttgca atttgatact
     4081 catttaggtt tacattttca ctttttgtac tccaaaactg aactacttaa atctatactg
     4141 ctgcctcttt tttttagtac atgtccttca tcttcgcagg aagtgccaat atgattttaa
     4201 gaaaggtaat agacaaaaag tcaaaaagat aaaacagagt ttcttcttta caaagggaat
     4261 gtatgttcgt ataatggtat tgaggtgaag gttatggaga aaaaatatgg aacagtagat
     4321 aagatccgta ctttgcaagt tagtgccaga ccgttgatcc gtttcagttt aaatggggtc
     4381 aattgtttga ttgcttccca tagtttgaac ttcctagctg aagtagacga agggatgaag
     4441 ttagtcgtta caggttatta taataataga aagcaatttg ttgtgagatc ctatcatgtg
     4501 ttagggaaac ccaaaattgt ggtagaatat gagaaaagtc tatacccaag aaaaaaggtc
     4561 cgacaagatg aaagaataaa ctagaaaaca agcggctgag acaaaaatgt cctagccgct
     4621 tgttttggaa agatggatgc agacgatggg atttgaaccc acacgagcat acgctcacaa
     4681 gaacctcaat ctatatataa ctacatgtat ttaagtattt gtgatgaatc actaaacttt
     4741 ccaaactcat ttgagggata ttgcttaaag attgaacaga tcaattcgaa gccctattag
     4801 tattcgctca atggggggat cagaggccat aaaaagagac tagtcattac tagtctcagg
     4861 tagtggaatt gtaggatttt aaacgttcct ggagagtacc ggtatgtaac tcgaagaggt
     4921 gattatcata gtcgtaaaag taaattgaag aagcctcgcc tttcacacga gaacgtcctg
     4981 gttcgacagt taaacctaac atttggattt ttgagacaaa cgaatctata tcttgttcgt
     5041 caattttaaa agcaatatga ttgtatgtct ttggcagttt attcgacgaa ttctgcatta
     5101 ctgcaatcca taggtcctta actaagaaaa atttttctgg atataatgaa aatttttttg
     5161 ttgactatcg tagatttctt ttgcatcaaa taaggtattt aacaattaag tatttaaaag
     5221 ataactttta agttatcttt aagtaatgct taaagctatg ttatactcta ttaggaaata
     5281 taagaaggag tataaaaatg aacaaacata atagaaaaat aacgaataac aaagaactga
     5341 tcttgttact aatagggatt tggggtatct ttttactctt taatatgtat atgccgacta
     5401 tgagagcgga tgacgtagtt tatgcaagta gattagatga acttggctat ttgggtgctt
     5461 cgatagaaca ttataagaca tggagttcaa gaattattat agaactattc ttaatgttct
     5521 tttctaaaca ctttatgcta tggaaattat taaattctac gattatgtta ggaattgtag
     5581 tattactttg caagtatgtt gagagtataa aatattttgt gtaaatgaaa aaatccat
//
LOCUS       DAZKOB010000313         4919 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.guided.150, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000313 DAZKOB010000000
VERSION     DAZKOB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.guided.150"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..515
                     /locus_tag="ACG8M6_003159"
     CDS             <1..515
                     /locus_tag="ACG8M6_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0458710.1"
                     /translation="TAPSSVNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVM
                     IFGDMQCYEYGEEIYNQAYESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSL
                     AAMQFMLVARDHGYETNPIGGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVR
                     LAPEKITMFE"
     gene            869..2347
                     /gene="msr(C)"
                     /locus_tag="ACG8M6_003160"
     CDS             869..2347
                     /gene="msr(C)"
                     /locus_tag="ACG8M6_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0458711.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2394..2840
                     /locus_tag="ACG8M6_003161"
     CDS             2394..2840
                     /locus_tag="ACG8M6_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0458712.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3039..3554)
                     /locus_tag="ACG8M6_003162"
     CDS             complement(3039..3554)
                     /locus_tag="ACG8M6_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0458713.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3671..4873
                     /locus_tag="ACG8M6_003163"
     CDS             3671..4873
                     /locus_tag="ACG8M6_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0458714.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1488 a          825 c         1070 g         1536 t
ORIGIN      
        1 cgactgcacc ttcttctgtc aatatgaaac catggcgctt tgtggtcgct gaaagcgagg
       61 aagcaaaaga aatcttgcgt cctttgatcc gtttcaatac gagacaaaat gatacttctt
      121 ctgcaatggt catgattttt ggcgatatgc aatgttacga atatggcgaa gaaatctaca
      181 accaagctta cgaatcaggg aagatgccaa aagaagtcag agatcaacaa ttagctgcga
      241 tcattcctta ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc aaagtcgatt
      301 ctagcttagc ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat gaaacaaatc
      361 caattggtgg attcgaagca gatcagttgg cagaggcatt tggtttggat aaagaccgtt
      421 atgttccagt tattatttta tcaataggaa aagcggtaga agaaggttac gagtctgttc
      481 gtttagcacc agaaaagatc actatgttcg aataaaaggt tttcagttac tattcagtag
      541 ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac
      601 tgcatcgatg aaattacgtt tcgaactttt gaataacaac taaataaatt caaaagacat
      661 gtataaacaa caatttcatg gatggagtat gtccattttt tcgaacgaca gtagaatagg
      721 tgaaagctat gacagatttg gtgatttagt catagttatt tcgttcagag taggaaaaga
      781 tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg
      841 ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct
      901 acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca
      961 agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat
     1021 agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct
     1081 gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt
     1141 ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat
     1201 cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac
     1261 ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac
     1321 gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga
     1381 agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa
     1441 ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg
     1501 attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag
     1561 taaaaagaaa aagcaacaaa tcagactgga tcggttgtct tcctctaaac aaaaagacag
     1621 tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg
     1681 agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga
     1741 actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac
     1801 attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga
     1861 aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct
     1921 tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga
     1981 agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag
     2041 tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag
     2101 tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt
     2161 gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct
     2221 aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg
     2281 tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa
     2341 tttttaaatg tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta
     2401 caaataaaaa agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga
     2461 tgagcgcgta taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc
     2521 tagtgcctgg attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga
     2581 aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta
     2641 tttcatgctt gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg
     2701 aacaaggaat acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga
     2761 ttgccgcttt accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag
     2821 caattcaaag taatgaatga tggaacactg ttatttgaaa aaaggccttg gcatgctttt
     2881 tgtcatagtc tcttgaatgc agttctagaa aaatagtgat ccgaacgata ggttttggcc
     2941 tgtcgttcgg atcgcttttt cttttccaac tgtattgctc ttttattgaa gataatttgc
     3001 tcttcaatag atatttctag aaaggttctg tttatccctt actcaaaaaa ctgctgcatt
     3061 tgttctctta cttgctgaca ccattggagt tcctgctgac aaagcataat tctgcgctgg
     3121 atcacaacat aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt caattttttt
     3181 tgattttttt gcatgatttt gttcagctgt tgttctctag tgaatagcag tccagtagct
     3241 gttgttttat cgagcgtcga aaaagcatag atttttgcta gaaattcatc tttttgcgta
     3301 ggagcgggag tttcctcttt gatccattcc tctacaagag cacgtccttc atcagtcaag
     3361 tggtagatct ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata accctgctgg
     3421 tctaacttgg caagcgttgt atagatctga ctgtgatggg cttcccaaaa aattgtgatc
     3481 agctgcttca attcataccc agaacgtggt ttggataaaa gcacgcataa taatatatag
     3541 gctaaagtat tcataaaaat cctacctctc gtcttatttt ctttattata tcacagtaaa
     3601 gtttccacgt tttacatgtg catattgaca tgtaaaataa atcatgtatg atgctaatag
     3661 agagaaaggg atggaaatat tggagaatca aatagatata caagaacaaa caaatgaaga
     3721 aacaaagggg aaaagttcag cgctagttgt agcagctacc tgtatgatcg cttttatggg
     3781 agtgggtcta gtagacccaa tcttaaaaac gattgctgca cagttaaacg ctacgccagc
     3841 tgaaacgacc ctgttattta ctagttacat gttagtcact ggtgtggtga tgctatttac
     3901 aggtttctta tctagtcgga tcggtttgaa aaaaacattg atgacaggat tgtttatcat
     3961 cgttgtattc gctggtcttg gcggattatc tgggaatatc ggaatgctga ttgggttacg
     4021 tgccggctgg ggtgtcggga atgctttatt tgtctctact gctttagcaa cgatcgtcag
     4081 tattttagtt ggtaacacag aaaaagcaat catgatgtat gaagcagcac ttggctgcgg
     4141 aatggctgtt ggccctctag tcggtggggt cttgggcagt ggttcatggc gttatccatt
     4201 ttttggggta gcatttttga tgttttgcgg ttttatagcc ctagcggtac ttttacctga
     4261 aactgagaaa ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt taagcaatca
     4321 taaacttcgt tcagttggac taatcgcatt gttatataat ttcggattct ttactttact
     4381 tgcgtatgcg ccatttttat tgattggcta ttctgaatta gaagtcggtt ttgtattttt
     4441 tggctggggt gtgcttctgg ctattgcttc gattttcgtt gcaccggctc ttgagcggaa
     4501 gtttacgact tatcggacaa ttttagcagc atttgtctta ttcattattt gtttgctgtt
     4561 attaggagct ggaacttcta tgccagtcct tgtaccgatc agcattgtgt tagcaggatt
     4621 tttccaagga attatcaata cactgttgac gacgattgct atggaaatac caggtctgca
     4681 acgaaacgtt gcttcgtcca gctatagttt tgtccgcttt ttcggtggag ctttagcacc
     4741 atttattgca ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt attttgcagg
     4801 tgccattgtt cttatcagtg tatgttgtat catttatcac cgtaattatt ttgctacaaa
     4861 tgaagaagaa taagaaatca gaggttgtga aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DAZKOB010000314         4802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.guided.151, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000314 DAZKOB010000000
VERSION     DAZKOB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.guided.151"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8M6_003164"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG8M6_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0458715.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACG8M6_003165"
     CDS             763..1917
                     /locus_tag="ACG8M6_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0458716.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..3100
                     /locus_tag="ACG8M6_003166"
     CDS             2132..3100
                     /locus_tag="ACG8M6_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0458717.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8M6_003167"
     CDS             3093..4124
                     /gene="vanA"
                     /locus_tag="ACG8M6_003167"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0458718.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4130..4738
                     /locus_tag="ACG8M6_003168"
     CDS             4130..4738
                     /locus_tag="ACG8M6_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0458719.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1481 a          921 c         1119 g         1281 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcgaccg tggcaaggta ctcagcgaca tgacagttgg
     2581 tgtggtggga acgggccaga taggcaaagc ggttattgag cggctgcgag gatttggatg
     2641 taaagtgttg gcttatagtc gcagccgaag tatagaggta aactatgtac cgtttgatga
     2701 gttgctgcaa aatagcgata tcgttacgct tcatgtgccg ctcaatacgg atacgcacta
     2761 tattatcagc cacgaacaaa tacagagaat gaagcaagga gcatttctta tcaatactgg
     2821 gcgcggtcca cttgtagata cctatgagtt ggttaaagca ttagaaaacg ggaaactggg
     2881 cggtgccgca ttggatgtat tggaaggaga ggaagagttt ttctactctg attgcaccca
     2941 aaaaccaatt gataatcaat ttttacttaa acttcaaaga atgcctaacg tgataatcac
     3001 accgcatacg gcctattata ccgagcaagc gttgcgtgat accgttgaaa aaaccattaa
     3061 aaactgtttg gattttgaaa ggagacagga gcatgaatag aataaaagtt gcaatactgt
     3121 ttgggggttg ctcagaggag catgacgtat cggtaaaatc tgcaatagag atagccgcta
     3181 acattaataa agaaaaatac gagccgttat acattggaat tacgaaatct ggtgtatgga
     3241 aaatgtgcga aaaaccttgc gcggaatggg aaaacgacaa ttgctattca gctgtactct
     3301 cgccggataa aaaaatgcac ggattacttg ttaaaaagaa ccatgaatat gaaatcaacc
     3361 atgttgatgt agcattttca gctttgcatg gcaagtcagg tgaagatgga tccatacaag
     3421 gtctgtttga attgtccggt atcccttttg taggctgcga tattcaaagc tcagcaattt
     3481 gtatggacaa atcgttgaca tacatcgttg cgaaaaatgc tgggatagct actcccgcct
     3541 tttgggttat taataaagat gataggccgg tggcagctac gtttacctat cctgtttttg
     3601 ttaagccggc gcgttcaggc tcatccttcg gtgtgaaaaa agtcaatagc gcggacgaat
     3661 tggactacgc aattgaatcg gcaagacaat atgacagcaa aatcttaatt gagcaggctg
     3721 tttcgggctg tgaggtcggt tgtgcggtat tgggaaacag tgccgcgtta gttgttggcg
     3781 aggtggacca aatcaggctg cagtacggaa tctttcgtat tcatcaggaa gtcgagccgg
     3841 aaaaaggctc tgaaaacgca gttataaccg ttcccgcaga cctttcagca gaggagcgag
     3901 gacggataca ggaaacggca aaaaaaatat ataaagcgct cggctgtaga ggtctagccc
     3961 gtgtggatat gtttttacaa gataacggcc gcattgtact gaacgaagtc aatactctgc
     4021 ccggtttcac gtcatacagt cgttatcccc gtatgatggc cgctgcaggt attgcacttc
     4081 ccgaactgat tgaccgcttg atcgtattag cgttaaaggg gtgataagca tggaaatagg
     4141 atttactttt ttagatgaaa tagtacacgg tgttcgttgg gacgctaaat atgccacttg
     4201 ggataatttc accggaaaac cggttgacgg ttatgaagta aatcgcattg tagggacata
     4261 cgagttggct gaatcgcttt tgaaggcaaa agaactggct gctacccaag ggtacggatt
     4321 gcttctatgg gacggttacc gtcctaagcg tgctgtaaac tgttttatgc aatgggctgc
     4381 acagccggaa aataacctga caaaggaaag ttattatccc aatattgacc gaactgagat
     4441 gatttcaaaa ggatacgtgg cttcaaaatc aagccatagc cgcggcagtg ccattgatct
     4501 tacgctttat cgattagaca cgggtgagct tgtaccaatg gggagccgat ttgattttat
     4561 ggatgaacgc tctcatcatg cggcaaatgg aatatcatgc aatgaagcgc aaaatcgcag
     4621 acgtttgcgc tccatcatgg aaaacagtgg gtttgaagca tatagcctcg aatggtggca
     4681 ctatgtatta agagacgaac cataccccaa tagctatttt gatttccccg ttaaataaac
     4741 ttttaaccgt tgcacggaca aacggttctg ttgcaaagtt ttaaataaag aataaaatcc
     4801 ct
//
LOCUS       DAZKOB010000315         4599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.guided.155, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000315 DAZKOB010000000
VERSION     DAZKOB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.guided.155"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..737
                     /locus_tag="ACG8M6_003169"
     CDS             <1..737
                     /locus_tag="ACG8M6_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0458720.1"
                     /translation="SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVED
                     AIIRLAQMARAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNG
                     AEKLLGRGDMLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEV
                     ETTGAPEQPQDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGP
                     SEGSKPRKVFIEQEETSETLPDDNDM"
     gene            967..2034
                     /gene="rlmN"
                     /locus_tag="ACG8M6_003170"
     CDS             967..2034
                     /gene="rlmN"
                     /locus_tag="ACG8M6_003170"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0458721.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            2024..2230
                     /locus_tag="ACG8M6_003171"
     CDS             2024..2230
                     /locus_tag="ACG8M6_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0458722.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(2227..3330)
                     /locus_tag="ACG8M6_003172"
     CDS             complement(2227..3330)
                     /locus_tag="ACG8M6_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0458723.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3883..4431
                     /gene="aac(6')"
                     /locus_tag="ACG8M6_003173"
     CDS             3883..4431
                     /gene="aac(6')"
                     /locus_tag="ACG8M6_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0458724.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1510 a          787 c          961 g         1341 t
ORIGIN      
        1 tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa
       61 ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca
      121 atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata
      181 tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg
      241 taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt
      301 ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga
      361 ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc
      421 aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag
      481 ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac
      541 ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt
      601 acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag
      661 aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg
      721 acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa
      781 gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta
      841 tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt
      901 attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa
      961 aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt
     1021 atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa
     1081 cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg
     1141 gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca
     1201 gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa
     1261 gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt
     1321 tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag
     1381 atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc
     1441 gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat
     1501 atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt
     1561 ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt
     1621 tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc
     1681 ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc
     1741 acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta
     1801 gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca
     1861 gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat
     1921 gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat
     1981 gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat
     2041 cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa
     2101 cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac
     2161 cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc
     2221 aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc
     2281 tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat
     2341 atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt
     2401 catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa
     2461 caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc
     2521 gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat
     2581 agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc
     2641 cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg
     2701 cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc
     2761 cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc
     2821 atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat
     2881 gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt
     2941 atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa
     3001 gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc
     3061 gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt
     3121 tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa
     3181 agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac
     3241 ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt
     3301 ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg
     3361 gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct
     3421 aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc
     3481 taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag
     3541 tatttctacc gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt
     3601 attatatgaa aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat
     3661 aatctatatc atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt
     3721 tcctattttg aataaagtgt ttctgtataa tttttttatt ttataagtaa gataaataat
     3781 gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt
     3841 caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt
     3901 gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg
     3961 gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc
     4021 gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac
     4081 ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa
     4141 ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg
     4201 atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat
     4261 gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc
     4321 tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa
     4381 ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag
     4441 gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttacttcttg
     4501 aatttttgtg aaattccaac ttatttccga agaagctact tttggaacac cgtgtaccag
     4561 aggttgtgaa aaagtcgctt agtttcgagt agtaagaaa
//
LOCUS       DAZKOB010000316         3278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.guided.185, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000316 DAZKOB010000000
VERSION     DAZKOB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.guided.185"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..188
                     /locus_tag="ACG8M6_003174"
     CDS             3..188
                     /locus_tag="ACG8M6_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458725.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="ACG8M6_003175"
     CDS             203..1072
                     /locus_tag="ACG8M6_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0458726.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ACG8M6_003176"
     CDS             1053..1787
                     /locus_tag="ACG8M6_003176"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0458727.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ACG8M6_003177"
     CDS             1820..2728
                     /locus_tag="ACG8M6_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0458728.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="ACG8M6_003178"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="ACG8M6_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAZKOB010000317         3240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.guided.186, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000317 DAZKOB010000000
VERSION     DAZKOB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.guided.186"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..244
                     /locus_tag="ACG8M6_003179"
                     /pseudo
     CDS             197..244
                     /locus_tag="ACG8M6_003179"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            260..2179
                     /gene="tet(M)"
                     /locus_tag="ACG8M6_003180"
     CDS             260..2179
                     /gene="tet(M)"
                     /locus_tag="ACG8M6_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0458729.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2298..2465
                     /locus_tag="ACG8M6_003181"
     CDS             2298..2465
                     /locus_tag="ACG8M6_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0458730.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2525..2878)
                     /locus_tag="ACG8M6_003182"
     CDS             complement(2525..2878)
                     /locus_tag="ACG8M6_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0458731.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT         1102 a          494 c          626 g         1018 t
ORIGIN      
        1 gagttaataa atatgcggca aggtattctt aaataaactg tcaatttgat agtgggaaca
       61 aataattgga tgtccttttt taggagggct tagttttttg tacccagttt aagaatacct
      121 ttatcatgtg attctaaagt atccggagaa tatctgtatg ctttgtatgc ctatggttat
      181 gcataaaaat cccagtgata aaagtattta tcactgggat ttttatgccc ttttgggttt
      241 ttgaatggag gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc
      301 aggaaaaact accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg
      361 aagcgtggac aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat
      421 tacaattcag acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga
      481 cacgccagga catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg
      541 ggcaattcta ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca
      601 tgcacttagg aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg
      661 aattgattta tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat
      721 caaacagaag gtagaactgt atcctaatat gtgtgtgacg aactttaccg aatctgaaca
      781 atgggatacg gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc
      841 attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt gttctctgtt
      901 ccctctttat catggaagtg caaaaagtaa tatagggatt gataacctta tagaagttat
      961 tactaataaa ttttattcat caacacatcg aggtccgtct gaactttgcg gaaatgtttt
     1021 caaaattgaa tatacaaaaa aaagacaacg tcttgcatat atacgccttt atagtggagt
     1081 actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag ttacagaaat
     1141 gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg gagaaattgt
     1201 tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa aactattgcc
     1261 acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg aaccgagtaa
     1321 acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata gtgatccgct
     1381 tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt
     1441 acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa
     1501 agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat
     1561 cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt
     1621 gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcgtttca
     1681 aaatgcagtt atggagggga tacgctatgg ctgtgaacaa ggattgtatg gttggaatgt
     1741 gacggactgt aaaatctgtt ttaagtatgg cttatactat agccctgtta gtaccccagc
     1801 agattttcgg atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga
     1861 attgttagag ccatatctta gttttaaaat ttatgcgcca caggaatatc tttcacgagc
     1921 atacaacgat gctcctaaat attgtgcgaa catcgtagac actcaattga aaaataatga
     1981 ggtcattctt agtggagaaa tccctgctcg gtgtattcaa gaatatcgta gtgatttaac
     2041 tttctttaca aatggacgta gtgtttgttt aacagagtta aaagggtacc atgttactac
     2101 cggtgaacct gtttgccagc cccgtcgtcc aaatagtcgg atagataaag tacgatatat
     2161 gttcaataaa ataacttagt gtattttatg ttgttatata aatatggttt cttgttaaat
     2221 aagatgaaat attttttaat aaagatttga attaaagtgt aaaggaggag atagttatta
     2281 taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta aagataaggg
     2341 aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa gaaaatttaa
     2401 ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag acgcagagcc
     2461 gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt atggattctt
     2521 ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat tccattaagg
     2581 atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt cgggtgcttt
     2641 ttaccaagtt attagcaggt aagaaaaatt catcaacgga aacatgaagt aacgatacaa
     2701 ggtcataaag aacttgtatg ctggggtgtt gccctttatt ttcaatatta gttaagtacc
     2761 gtgggtcaat ttcaatcaat gctcccactt gttcacgagt taaacctcgt ttcaatcgag
     2821 cttctttaat ggctaaacca aaggctctaa aatcatattt atcttctttt ttacgcatag
     2881 tagaccacct ctatacattt tattgttcct actgaattaa aaacaggtat agaaaaacgt
     2941 gttatatggt ttataggttt atatttaata aaaagcacta ctaaacgcca ataaaaaaaa
     3001 ccgttatatg gtagtgctat ttacgctgtt aaaatattgt atattacttc caaatggcgg
     3061 tttgttggag gtcaacgtcg ccatgaagta catcatatac aataaatttc cttacattgg
     3121 gttcttgtca aaaaaagtcg tctatctgca atagataagt acgtccacca atgtggtttt
     3181 ataaatcata tagatagaat aacagaagca tgtaaacaga gaaataaatc tgtttatatg
//
LOCUS       DAZKOB010000318         1527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.guided.232, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000318 DAZKOB010000000
VERSION     DAZKOB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.guided.232"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..305
                     /locus_tag="ACG8M6_003183"
                     /pseudo
     CDS             36..305
                     /locus_tag="ACG8M6_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            824..1108
                     /locus_tag="ACG8M6_003184"
     CDS             824..1108
                     /locus_tag="ACG8M6_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0458732.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
BASE COUNT          576 a          220 c          271 g          460 t
ORIGIN      
        1 gtatagtatt ttacacagga ttatggactt gacttaaaga aatcggattt ttacctaagt
       61 ttgcttttgt actggtttta agtcttactt ttgaaataat tcaatttatc ttcgctattg
      121 gagcgacaga cataacagat gtaattacaa atactgttgg aggctttctt ggactgaaat
      181 tatatggttt aagcaataag catatgaatc aaaaaaaatt agacagagtt attatttttg
      241 taggtatact tttgctcgta ttattgctcg tttaccgtac ccatttaaga ataaattacg
      301 tgtaagatgt ctaaatcaag caatctgatc tttcatacac ataaagatat tgaatgaatt
      361 ggattagatg gaaaacggga tgtggggaaa ctcgcccgta ggtgtgaagt gaggggaaaa
      421 ccggtgataa agtaaaaagc ttacctaaca ctatagtaac aaagaaagcc caattatcaa
      481 ttttagtgct gaggaattgg tctctttaat aaatttcctt aacgttgtaa atccgcattt
      541 tcctgacggt acccccacta gcagccatgc tttttttgaa tctttgtagg acgttttaaa
      601 gagaattacc cactttaaaa ttaaacatgc gcttagaatc gctttaggaa acacgatcca
      661 gtccaataat cgtcgataaa aacttttgaa aaaggttggt gaaattacct acttttggaa
      721 taatcacaaa tcacaagtga ttaatcactt gtttattaag atattaaaag ctataattta
      781 aataaagcgt gaattttatt acacaaaaag aggggggaga aacttggaac tagcatttag
      841 agaaagctta aaaaagatga gaggtaccaa atcaaaagaa aaattctccc aagaattaga
      901 aatgagtaga tcaaattatt cacgaataga atcaggaaaa tcagatccaa ccataaaaac
      961 actagaacaa attgcaaagt taactaactc aacgctagta gtggatttaa tcccaaatga
     1021 gccaacagaa ccagaaccag aaacagaatc agaacaagta acattggatt tagaaatgga
     1081 agaagaaaaa agcaatgact tcgtgtgaat aatgcacgaa atcgttgctt atttttttta
     1141 aaagcggtat actagatata acgaaacaac gaactgaata gaaacgaaaa aagagccatg
     1201 acacatttat aaaatgtttg acgacatttt ataaatgcat agcccgataa gattgccaaa
     1261 ccaacgctta tcagttagtc agatgaactc ttccctcgta agaagttatt taattaactt
     1321 tgtttgaaga cggtatataa ccgtactatc attatatagg gaaatcagag agttttcaag
     1381 tatctaagct actgaattta agaattgtta agcaatcaat cggaaatcgt ttgattgctt
     1441 tttttgtatt catttataga aggtggagtt tgtatgaatc atgatgaatg taaaacttat
     1501 ataaaaaata gtttattgga gataaga
//
LOCUS       DAZKOB010000319         1453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.guided.237, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000319 DAZKOB010000000
VERSION     DAZKOB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.guided.237"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..1040
                     /locus_tag="ACG8M6_003185"
     CDS             129..1040
                     /locus_tag="ACG8M6_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0458733.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1193..1414
                     /locus_tag="ACG8M6_003186"
                     /pseudo
     CDS             1193..1414
                     /locus_tag="ACG8M6_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
BASE COUNT          529 a          197 c          284 g          443 t
ORIGIN      
        1 agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa acgagattgt
       61 gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag ttaaggagga
      121 gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata tacttaggtt
      181 atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa aattatgatc
      241 aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa gagaaaacaa
      301 ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa tatcctgttc
      361 gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa ttaataaatg
      421 gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa aaattttcag
      481 agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat agtggctatc
      541 gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat gccttaccag
      601 caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc ttgacgaaaa
      661 tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac gggttcattt
      721 tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca tggcatattc
      781 gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt ctcgaggaat
      841 atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg gaaaaatatg
      901 agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact aatcttcgtt
      961 atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc ggatcaacac
     1021 atactaattc aaggaggtaa ggatggcgga atgaaaccaa cgaaattaat gaacagcatt
     1081 attgtactag cacttttggg gtaacgttag ctttttaatt taaaacccac gttaactagg
     1141 acattgctat actaatgata caacttaaac aaaagaatta gaggaaatta tattgggaaa
     1201 aatattatct agaggattgc tagctttata tttagtgaca ctaatctggt tagtgttatt
     1261 caaattacaa tacaatattt tatcagtatt taattatcat caaagaagtc ttaacttgac
     1321 tccatttact gctactggga atttcagaga gatgatagat aatgttataa tctttattcc
     1381 atttggcttg cttttgaatg tcaattttaa agaaagataa agtccatata attgtgtaaa
     1441 agataaaagg cca
//
LOCUS       DAZKOB010000320         1149 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.guided.251, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000320 DAZKOB010000000
VERSION     DAZKOB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.guided.251"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..350
                     /locus_tag="ACG8M6_003187"
     CDS             <1..350
                     /locus_tag="ACG8M6_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HFQ0458734.1"
                     /translation="WTESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMI
                     PWIDFSSFNLNIGNNSSFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMN
                     EFQDIIHRVDDWI"
     gene            complement(1043..1149)
                     /locus_tag="ACG8M6_003188"
                     /pseudo
     CDS             complement(1043..1149)
                     /locus_tag="ACG8M6_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
BASE COUNT          385 a          146 c          188 g          430 t
ORIGIN      
        1 tttggactga atctgataaa aacttcattt ctttttataa taattataaa aatgacttgc
       61 ttgaatataa agataaagaa gaaatgtttc ctaaaaaacc gatacctgaa aacaccatac
      121 cgatttcaat gattccttgg attgatttta gttcatttaa tttaaatatt ggtaacaata
      181 gcagcttttt attgcctatt attacgatag gtaaatttta tagtgagaat aataaaattt
      241 atataccagt tgctctgcaa cttcatcatt ctgtatgtga tggttaccat gcttcactat
      301 ttatgaatga atttcaagat ataattcata gggtagatga ttggatttag tttttagatt
      361 ttgaaagtga atttaatttt atacacgtaa gtgatcataa aatttatgaa cgtataacaa
      421 ccacattttt tggttgcttg tggttttgat tttgaatttg gttttgaact tatggactga
      481 tttattcagt ccattttttg tgcttgcaca aaaactagcc tcgcagagca cacgcattaa
      541 tgacttatga aacgtagtaa ataagtctag tgtgttatac tttacttgga agatgcaccg
      601 aataaaaaat attgaagaac aactagcaaa agattttaaa gagttatttt attttaagtc
      661 tttataacat gagtgaagcg aatttttaaa tttcgataga aatttttaca tcaaaaagcc
      721 ccctgtcaaa attgacgaag ggggtttttt ggcgcacgct tttcgttaga aatatacaag
      781 attgaaaatc gtgtataagt gcgccctttg ttttgaactt agcacgttac atcaattttt
      841 taaaatgatg tataagtgcg cccttttaaa ttttgagtga ttatattttt tgagttagaa
      901 aaagggattg ggaaaatttc ccaaaataat ttaaaaaata agcaaaaatt ttcgatagag
      961 aatgtgctat tttttgtcaa aggtgtatac cttgactgtg cttgctgtta cattaagttt
     1021 atttttaagt tattaaaaaa gaaatagctt ttaaagtttg gctcgctgtc gctttataaa
     1081 gctgattgac ttttgattgc aaactactta aagaaaacaa actcggacta ttcgttttct
     1141 tctctttgg
//
LOCUS       DAZKOB010000321         1148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3469
            SAMEA115706741-rid22569983.guided.252, whole genome shotgun
            sequence.
ACCESSION   DAZKOB010000321 DAZKOB010000000
VERSION     DAZKOB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706741
            Sequence Read Archive: ERR13196475
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287755.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287755.1
            Genome Coverage       :: 116x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 07:06:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,190
            CDSs (total)                      :: 3,129
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 61
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 40 of 98
            Pseudo Genes (incomplete)         :: 54 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 16 of 98
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706741-rid22569983.guided.252"
                     /isolate="VRE3469"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..76
                     /locus_tag="ACG8M6_003189"
     CDS             26..76
                     /locus_tag="ACG8M6_003189"
                     /inference="COORDINATES: protein motif:HMM:NF018061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0458735.1"
                     /translation="MWTLSKQTKNSDYADK"
     gene            201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8M6_003190"
     CDS             201..938
                     /gene="erm(B)"
                     /locus_tag="ACG8M6_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0458736.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          199 c          185 g          343 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcg aaacagacta aaaacagtga
       61 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
      121 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      181 atcacttcag gagagattac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      241 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      301 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      361 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      421 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      481 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      541 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      601 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      661 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      721 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      781 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      841 caatgaaaca cgccaaagta aacaatttaa gtaccattac ttatgagcaa gtattgtcta
      901 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttttaaa
      961 tttggaaagt tacacgttac taaagggaat ggagataaat tattagatat actactgaca
     1021 gcttccaaga agctaaagag gtccctagcg cctacgggga atttgtatcg ataaggggta
     1081 caaattccca ctaagcgctc gggacccctt gtaggaaaat gtcctaagtg tggcaacaat
     1141 attgtatt
//
